LOCUS       DATCBB010000001         2293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_129771, whole genome shotgun sequence.
ACCESSION   DATCBB010000001 DATCBB010000000
VERSION     DATCBB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129771"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>2293
                     /locus_tag="VII37_00005"
     CDS             12..>2293
                     /locus_tag="VII37_00005"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5068125.1"
                     /translation="MTDHIRTLSQLTDAYVSCYPNAGLPDEDGKYLETPTTLAAQLEK
                     FVDHGWLNFIGGCCGTTEKHIRAISQMVEGKKPRRRPSETHRAIYSGIETIEADDQTR
                     PLLVGERTNVIGSRLFKNLVAEEKWEEASEVARRQVRGGAHIVDVCLQSTERDEKKDI
                     EPFYEKLIRKVKTPVMIDTTDPSAIEIALTYCQGKSIINSINLEDGEEKFERVVPLAH
                     DFGAAVVVGCIDEDKEQAQAFTRERKLAIAQRSYKLLTEKYGLGPEDIIFDPLVFPCA
                     TGDENYIGGAVETVEGIRLIKQALPDTRTILGISNVSFGLPPGAREVVNSVFLYYCTK
                     AGLDLAIVNTEKLERFASIPEHERRLAENLLFSHPPTNVPAEHPQAALLANAPKDWRQ
                     QSKEQKAAVNQYYIAAIAEHFRTATKKQKISAADLPLDERLANYIIEGTRDGLVADLE
                     RKRGEGVAPLDIVNGPLMAGMAEVGRLFNANELIVAEVLQSAEAMKAAVSHLEQFMEK
                     ADTAKRGKVVLATVKGDVHDIGKNLVEIIFKNNGYDVVNLGIKVPPEELIKAYKQHHP
                     DAIGLSGLLVKSAQQMVITASDLKDAGIMVPLLVGGAALSAKYTQNKIGPTYGQAVFY
                     AKDAMTGLRLMNELMDPGTREEVMHQHTFSGNGVSVSTTVVVNAAPAVARSPKVRVDL
                     PIPEVATLDRKVRLVPDLREIWSYINPYMLYGRHLGFRGDFVKRYAERDAKAVELYES
                     MEEVKAEAAQFMKVRAVWQF"
BASE COUNT          567 a          506 c          738 g          482 t
ORIGIN      
        1 ggccagagtt catgaccgat cacattcgta cgctgagcca actgacggat gcgtacgtgt
       61 cgtgctaccc caatgccggc ttgccagacg aagatggtaa atatttggag acgccgacaa
      121 cgctggccgc gcaactggaa aaatttgttg accatggatg gttgaatttt attggtgggt
      181 gctgcggaac gacggaaaag catattcgag cgatttcgca gatggtggaa ggcaagaagc
      241 cgcgacggcg gccgtctgaa actcatcgcg ctatttattc tgggattgaa acgatcgaag
      301 cggatgacca aacgcgacca ttgctggtgg gcgaacgcac caacgtgatc gggtcgcgat
      361 tatttaaaaa tctggttgcc gaagaaaagt gggaagaggc cagtgaagtg gcgcggcggc
      421 aggttcgcgg cggcgcgcac attgtggacg tatgcctgca aagcacggag cgtgacgaga
      481 agaaagatat tgagccgttt tatgaaaaat tgattcgcaa ggtgaagacg ccggtgatga
      541 tcgacactac cgatccttcg gcgattgaga ttgcgctgac gtattgccag ggcaagtcga
      601 tcattaactc catcaacctg gaggatgggg aagagaaatt cgaacgggta gtgccgctgg
      661 cgcacgattt cggggcggcc gtggtggtgg gctgcattga tgaagataaa gagcaggcgc
      721 aggcgtttac ccgcgagcgg aaattggcga ttgcgcagcg cagctacaaa ctgctcaccg
      781 agaaatatgg cttagggccg gaagacatta tttttgatcc actggtgttt ccgtgcgcga
      841 ccggagacga aaattatatt ggcggggcgg ttgaaacggt tgagggcatt cgattaatca
      901 agcaggcgtt gccggatacc cggacgatct tgggaatatc gaatgtgtct tttggattgc
      961 cgcctggcgc gcgcgaagtc gttaattcgg tttttcttta ttactgcacc aaggctgggc
     1021 tcgatctggc aatcgtgaat acggaaaagc tggagcggtt tgcctcgatt ccggagcacg
     1081 agagacggct ggctgaaaat ttattgttct cgcatccgcc gacaaatgtc ccagcggagc
     1141 atccacaagc cgcgctgcta gcgaatgcgc cgaaagattg gcgccagcag agtaaggaac
     1201 aaaaagccgc ggtgaaccaa tactacattg cggccatcgc ggagcatttt cggacggcta
     1261 ccaagaaaca aaaaatcagc gcggcggact tgccgctgga tgaacggctg gccaactaca
     1321 ttatcgaagg cacgcgtgac gggttggttg ctgatctgga gcgtaagcgc ggcgaaggcg
     1381 tcgcgccgct ggatattgtg aatgggccgc taatggccgg aatggcggaa gtgggacgat
     1441 tgtttaacgc caacgagctg atcgtcgcgg aggtgttgca atcggcggaa gcaatgaaag
     1501 cggcggtcag ccatcttgag caattcatgg aaaaggcgga caccgcgaag cgcggcaagg
     1561 tggtgctggc cacggtaaag ggagatgtgc acgacatcgg gaaaaatctg gtggagatta
     1621 tcttcaagaa caacggatac gatgttgtga atctggggat caaagtccca cccgaagaat
     1681 tgatcaaggc ttacaaacag catcatcctg atgcgattgg gctgtctgga ttgctggtga
     1741 agagcgcgca acagatggtc atcactgcga gcgatttgaa agatgcgggg atcatggtgc
     1801 cgttattggt gggaggcgcg gcgctttccg caaagtacac gcaaaacaag atcgggccaa
     1861 cgtacggcca ggcggttttt tacgctaagg atgcgatgac cggattgcgg ctgatgaacg
     1921 agctgatgga tccgggcact cgcgaagagg tgatgcacca gcacacgttc tctgggaatg
     1981 gcgtcagtgt gtcgacaacg gtggtggtca acgcagcgcc tgccgtcgcg cgctcaccaa
     2041 aagttcgcgt ggatctgccg atacctgagg tggcaacgct cgatcgcaag gtgcgattgg
     2101 ttcctgattt gcgagaaatc tggagctata tcaatcctta catgttgtat gggcggcatt
     2161 tgggttttcg cggagatttt gtgaagcgct atgcggagcg tgacgcgaag gctgtggagt
     2221 tgtacgaaag catggaagaa gtgaaagcgg aagcggcaca gtttatgaaa gtgcgcgcgg
     2281 tgtggcaatt ttt
//
LOCUS       DATCBB010000002         1501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_356885, whole genome shotgun sequence.
ACCESSION   DATCBB010000002 DATCBB010000000
VERSION     DATCBB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356885"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="VII37_00010"
     CDS             complement(<1..>1501)
                     /locus_tag="VII37_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY5068126.1"
                     /translation="KSKVAAKPNVILITIDTLRADHVGCYGAQMVKTPTLDAMAHDGI
                     VFERAISQVPLTWPSHAVILTGTYPFQNGVQDFTGQPLAPQFRSVAQAFKQADYATGA
                     VVSAFVLDRSWGLARGFDFYDDAFAAETFETKDAGLVDRRAGESVAHAIAWLNKTPRR
                     PFFLWLHLYDPHSPYDPPEPYRSEYRNHLYDGEIAYADHELGKLIAWLKQNHVYDSSL
                     IVALSDHGESLGEHGEDEHGFFVYNSTVHVPLIVKPPAESGIVAGRRREPVETASVAP
                     TLLKLSRLKPSSPSGDSIYAQFQSKALLPDNPAEGDAKPDTPSASSYSETLYPFSSFG
                     WSPLHALESLRFHFIEAPRPELFDLETDPGETRNIASEQPATVGVLREKLQAVLSHNP
                     FSRDADAGTGNLSPDAQEKLRALGYFGFRAAISPDALKHGLADPKDKLWEFNFILKAQ
                     DAFQRREDDQAEALLTEVQQKDPQIYVIPFLLGESALRRQNWEKAAEQL"
BASE COUNT          351 a          413 c          406 g          331 t
ORIGIN      
        1 tgcaattgct cagccgcttt ttcccagttc tgtctccgca atgccgactc gcccagcaga
       61 aatggaatca cgtaaatttg cggatctttt tgctggactt cagtaagcaa tgcttccgcc
      121 tgatcgtcct ccctacgctg aaaagcgtcc tgcgctttaa gaataaagtt aaattcccac
      181 agtttgtctt ttggatcggc aagcccgtgc tttaaagcgt cgggagaaat cgctgcgcga
      241 aatccgaaat atcccagcgc gcgcaacttt tcttgcgcgt ctggactgag attcccagta
      301 cccgcatccg catcgcgact gaaagggttg tgcgacaaaa ccgcctgcag cttttcccgc
      361 agtacgccaa ctgtcgcggg ttgctccgat gcaatgttcc gagtctcacc ggggtcagtt
      421 tccaggtcaa acagttccgg cctgggtgct tcgatgaaat gaaatcgcag actttccagt
      481 gcatgcaagg ggctccagcc gaacgaactg aaagggtaaa gggtctcgct gtaggaagag
      541 gcgctggggg tatctggttt cgcgtcgcct tccgccggat tatccggcag caaagctttg
      601 gactgaaact gagcataaat cgaatcgccc gaaggactcg acggtttcag tctagaaagt
      661 ttcaaaagcg tcggcgcgac cgaggcggtc tccaccggtt cacgacgccg gccggccaca
      721 atgccgcttt cagcgggcgg tttaacgatc agcggaacat gcacggttga gttgtacaca
      781 aaaaaaccgt gctcatcctc accatgctcg ccaagagatt cgccatgatc gctcagcgcg
      841 acaatcagcg aagaatcata aacatgattc tgcttgagcc atgcaattag cttgccgagt
      901 tcgtgatccg cataggcgat ctcgccatcg taaaggtggt tgcgatattc actacggtaa
      961 ggttcgggag gatcgtaagg gctgtgcgga tcataaaggt gcagccacaa aaaaaatggg
     1021 cgccgcggag tcttgttcaa ccatgcgatc gcgtgcgcta cgctttctcc ggcgcgacgg
     1081 tcgaccaggc ccgcatcttt tgtctcgaaa gtttctgcag caaacgcgtc gtcataaaag
     1141 tcaaagccgc gggccaatcc ccagctgcgg tcgagaacaa atgcgctaac tacagctccg
     1201 gtggcgtaat cggcctgctt gaaggcctgc gccaccgaac gaaattgcgg agcgagcggt
     1261 tgcccagtga aatcctgcac tccgttctga aagggatagg tgccggtcag tattaccgcg
     1321 tgcgaaggcc aggtgagcgg aacctgcgag atggcccgct caaacacgat gccatcatga
     1381 gccatcgcat ccagagtggg agtcttcacc atttgcgcgc cgtaacatcc cacgtggtcc
     1441 gcgcgaagcg tatcgatagt aatcaatata acattaggtt ttgcggcgac cttggacttt
     1501 a
//
LOCUS       DATCBB010000003         1907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_454243, whole genome shotgun sequence.
ACCESSION   DATCBB010000003 DATCBB010000000
VERSION     DATCBB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454243"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VII37_00015"
     CDS             complement(<1..746)
                     /locus_tag="VII37_00015"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEY5068127.1"
                     /translation="MLLVDNAFSVWRMSRSIKTVLSLGAVLLALSVAKTSSAQAAHDA
                     APSPQASQTFVLTDAKDLTEQGVKAEAVEYRGRKAIRLTAPAGVEDEFAFVNGTQFRD
                     GTIEVDIATKITTPPGVRMPGFTGIAFRARGDASHYELFYLRPGNAHAEDQAMRNHSA
                     QYTAEPGYSWEKLRRRWPFIYEAYADLQLDEWTKVKIEVHGRQARLYVNGSENPTLIV
                     DGLKGEDLQGAVALWGYAGEEAYFSNLRIS"
     gene            883..1758
                     /locus_tag="VII37_00020"
     CDS             883..1758
                     /locus_tag="VII37_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5068128.1"
                     /translation="MSSKTKTEVTVRVAKPEDSLMCGQICYDAFSTINATHNFPCDFP
                     GPEVAIGILSMMFSSPDFYCVVAESDGKIVGSNCLDERGAILGVGPITIDPSAQNLGV
                     GRKLMQAVMDRGHERGAPGIRLVQAAFHNRSLSLYMSLGFDIREPLSCLQGRTIERSI
                     PGCTVRPAAPGDEDACNALARRVHGFDRSLELAQAIGQGTARVVKRGERITGYTTDLA
                     FFGHTTAETNVDMQALIASVESFGGSGILVPSRNNVLIRWCLANGLRVVQPMTLMSTG
                     LYNEPSGAWLPSVSF"
BASE COUNT          386 a          555 c          554 g          412 t
ORIGIN      
        1 ttgctgattc tgaggtttga aaagtaggct tcctctcctg cataacccca cagcgcgacg
       61 gcgccttgca ggtcttcgcc cttcaatccg tccacgataa gagtggggtt ttctgacccg
      121 ttcacataca gcctcgcttg ccggccatgc acttcgatct tcaccttggt ccattcgtcc
      181 agttgcagat ccgcataagc ttcgtagata aaaggccacc gtcgccgcag cttttcccag
      241 ctataaccgg gctcggctgt atattgcgcc gagtggttgc gcatcgcctg gtcttccgcg
      301 tgagcgttgc ccggccgcag gtaaaacagc tcatagtgcg aggcatcacc acgggcgcgg
      361 aacgcaattc ctgtgaagcc tggcatcctc acgcccggtg gcgtggtgat cttggtggcg
      421 atatctacct cgatcgtgcc atcgcgaaac tgcgtgccgt tgacgaacgc aaattcgtcc
      481 tcgacccccg ccggcgcagt caagcggatc gctttgcggc cccgatattc gactgcctcg
      541 gcctttacac cttgttcggt caggtccttg gcatccgtga gcacaaaggt ctgcgatgcc
      601 tggggcgacg gagccgcatc atgagccgct tgtgcgctgc ttgtcttggc taccgataac
      661 gccagcagca cggctcccag agaaaggaca gtcttgatcg atcgggacat gcgccaaaca
      721 ctaaaggcgt tgtcaaccag cagcaagcgt cgtgtgacga atggaccaaa attgatgtga
      781 aatggatttc ggatcgacgc aaatacgcgc cactggagtt ttgagagagc ggcgcaaacg
      841 gctgctatac tcccattcga aaaaccagga gggtttgaaa ccatgtcgtc gaagacaaag
      901 acggaagtga ccgttcgggt cgcgaaaccg gaagacagcc tcatgtgcgg acagatttgc
      961 tacgacgctt tttctaccat caacgccact cacaatttcc cctgcgattt tcccggaccg
     1021 gaagttgcga tcggcatact ttcgatgatg ttttcgagtc cggattttta ttgcgtggtt
     1081 gcggaaagcg acggcaaaat cgtgggcagc aattgcctcg atgagcgtgg cgcgattctg
     1141 ggcgtaggcc cgatcactat cgatcccagc gcacagaatc tgggagtagg ccgcaagctc
     1201 atgcaggccg tgatggaccg aggccatgag cgtggcgcac ccggaatccg gctggtgcaa
     1261 gccgcgtttc acaatcgctc gctctcgctg tacatgtcgc taggcttcga tattcgcgag
     1321 ccactttcct gcttgcaggg acgaacgatt gagcggagta ttcccggatg cacggtgcga
     1381 ccggccgcac ccggcgatga ggacgcctgc aatgccctgg cgcgccgggt gcatggattc
     1441 gatcgaagtt tggagttggc acaggccatc gggcagggta cggcgcgcgt cgtgaagcgc
     1501 ggtgaacgca tcaccggata cacgacggac ctcgcgtttt tcggccacac caccgcggag
     1561 accaacgtgg acatgcaggc gttgatagct tcggtggaat cctttggtgg atcgggaatt
     1621 ctcgtgcctt cacgaaacaa cgttctgatc cgctggtgcc tggccaacgg attgcgcgtg
     1681 gtacagccga tgacgctgat gagtacgggc ctgtataacg agccctccgg agcctggctg
     1741 ccgtcagttt ctttttaggg ccagttcacg ccctaaaaca acgtgtcttt gggcagatag
     1801 tctttccatt cgcggctttc ggcgatgcgc ttgagcagcg caaaacgcct gcgcatcagg
     1861 ccgcgaatgt gcggcgccag cacccatcgt cccaccaggt cgtccag
//
LOCUS       DATCBB010000004         5473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_292033, whole genome shotgun sequence.
ACCESSION   DATCBB010000004 DATCBB010000000
VERSION     DATCBB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292033"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="glmS"
                     /locus_tag="VII37_00025"
     CDS             complement(<1..1472)
                     /gene="glmS"
                     /locus_tag="VII37_00025"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY5068129.1"
                     /translation="MCGIVGYIGPKKVVPVIIEGLRKLEYRGYDSAGIAVVTKDGKLE
                     IRRAAGKLRNLEEAIAKSPIEGTYGIGHTRWATHGRPTEENAHPHRDCSGQIVVVHNG
                     IIENYLELKEQLQREGHKFVTETDTEIVAHLVEKHTANNTPLEEAVRKSLKELRGIYA
                     LVFLSAKEPMKIVAARLGPPSVIGIGDGEYFVASDIPALLEHTRDMFFLADGDIAVLT
                     RDGVRVMDHDGRTVERPTQHVTWDPIMVEKGGYKHFMQKEIFEQPRAVRDTLLGRISQ
                     DTGKIFLDEMKISEEQFRTFENVRIVACGTSWHAGLAAKFMIERLARIPVEVDYGSEF
                     RYRDPILDSRTLTVCISQSGETADTLAAQREAKSKGSPTVAICNVMGSMITREAAGTI
                     PTHAGPEIGVASTKAFTAQLTALLLLAGYLGQVRGKVSPQCAIKLMQEITRIPHKMER
                     VLQTDEKGVYENLARQLFRYSDFLYLGRGIHYPIALEGAL"
     gene            complement(1601..1954)
                     /gene="purS"
                     /locus_tag="VII37_00030"
     CDS             complement(1601..1954)
                     /gene="purS"
                     /locus_tag="VII37_00030"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY5068130.1"
                     /translation="MRAPRWRCRALGKAHRHSEISRNLGRFRHRPKCYNRRMKAHVWV
                     MPKRTVLDPQGQTIQHALAGLGYDQVKDVRQGKFFVLDLAGWTQEEAQGQLERISKDV
                     LTNPVIEEYRFEIID"
     gene            complement(1899..2939)
                     /gene="add"
                     /locus_tag="VII37_00035"
     CDS             complement(1899..2939)
                     /gene="add"
                     /locus_tag="VII37_00035"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY5068131.1"
                     /translation="MNLGAQSNSLSADFLALPKAELHLHLEGSLQPETVCALAAKYGV
                     PASKQEVLERYAYGDFLEFIECFKWVSSLLRAPEDFTLALRDLGEQLLTQNVVYAEVT
                     LSIGVMLLRHQSAEKNFEAIVAAAESFRSRGLQLNFVFDAVRQFGTDAAMKVVEAAER
                     CASKSIVAFGIGGDELSVPAKEFRPVYEKAGAVGLRKLMHAGEVGGPEEIREAIELLG
                     AERIGHGIAAVRDPSLMDLLAERHIPLEICPQSNIRTGALAKLLGVDAAQIEQHPLPQ
                     FFRHGIPVVLSTDDPAMFHTNLRSEYENAARMGLQEAELTRMAKMSFEHAFIDESAKV
                     ALQGLGKSTSAF"
     gene            complement(2936..3733)
                     /locus_tag="VII37_00040"
     CDS             complement(2936..3733)
                     /locus_tag="VII37_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068132.1"
                     /translation="MIFVVLPHSARAWGRNGNKLVVNKAIETLPADTRAFFESNRALL
                     SQHVTDPLDTIATKAAERHNHFLYLDKYGRFPFDALPRNYKAAVNKYGKTKLQSNGLL
                     PWQIGVYSQKLTEDMKAGHWDEAKLDAAILANYVAEAHDPFNTTEDFDGHLTAQIGIN
                     ERFGTTLIDRYSSFFPMRPNDAAYISDPTDHAFEACLNSHSWLETILLADRNSKRGEN
                     SYNDEYFDHFYNAAAVILIRQLSDAATDVGSYWLTAWINAGRPQLPH"
     gene            complement(3825..>5473)
                     /locus_tag="VII37_00045"
     CDS             complement(3825..>5473)
                     /locus_tag="VII37_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5068133.1"
                     /translation="GLARYIEAQTAFHPRCANWIQFDPVEPGEPKSDWSVCVASDLFR
                     CKGIDQLLSDSENAEVHLKKSLGWVSLTALGIGAVIGSGIFTVIGTAIAGQHLQASSI
                     LNAPLLEYLIYHSASFGRPGAGPAIALSFVLVAIVCGFASVCYAELAAMIPIAGSAYT
                     YTYATMGELIAWIIGWDLILEYAVSNMAVCVGFSAHIVDLLDFFGIHPNPRWISPAYL
                     PTGMSDFQGNMLLDPGWHFGFNFPAFLIVMLLTVILIRGIRESAGANNIMVLLKITAI
                     LVFIGFASKLVHPANWHPFAPNGWPGILTGGSIVFFTYIGFDSVSTAAEECKRPQRDL
                     PIGIIATLIVCTLLYIGVVVVLTGLVPWQTLLDDAAPVVNTLKKLHFTGVRLVVLIGA
                     LLGMISSLLVFQLGQARVWFAMSRDGLLPRIFSRVHPRFQTPDFSTLMAGIVVGIPAG
                     LLDIGTLADLSNIGTLFAFALVAGGVLILRYREPERRRAFRAPGGPIAPILTILTCLL
                     LMAGLPITNWIRFFVWMIIGLVIYYNYGRKRSTLRAVARP"
BASE COUNT         1285 a         1567 c         1402 g         1219 t
ORIGIN      
        1 ttcagtgcgc cttcgagagc gatagggtaa tgaatgccgc ggccaaggta cagaaagtcg
       61 ctgtagcgaa ataattggcg agccagattt tcataaactc ctttttcgtc cgtttgcagc
      121 acgcgttcca ttttgtgcgg aatccgagtg atctcctgca tcaatttaat cgcgcactgc
      181 gggctgactt ttccgcgcac ctgcccaaga taaccggcca gcagcaacag cgcggtgagt
      241 tgcgctgtga acgctttggt ggacgccact ccaatctccg ggcccgcatg ggtgggaatc
      301 gttccggccg cttcacgggt gatcatcgat cccatcacgt tgcagatggc taccgttggc
      361 gaaccttttg acttggcttc gcgttgcgcg gcgagcgtat ccgccgtctc tcccgattgg
      421 ctgatgcaca ccgttaacgt cctggagtcc agtatcgggt cgcggtaacg aaactcgctg
      481 ccataatcga cttccactgg aatgcgcgcc agccgctcga tcatgaactt ggccgccagg
      541 ccggcgtgcc aactcgttcc gcaagcgacg atgcgcacat tctcaaaggt gcgaaattgc
      601 tcctcggaga ttttcatttc atcgaggaat attttgccgg tgtcttgcga aattcgaccc
      661 agcagcgtgt cgcgtacggc gcgcggctgc tcgaaaatct ctttctgcat gaaatgtttg
      721 tagccgcctt tttccaccat gatggggtcc caggtgacgt gctgcgtggg gcgttccacg
      781 gtgcggccat catggtccat cactctgacg ccgtcgcgag tgagcaccgc gatgtcgccg
      841 tcggcaagga agaacatgtc ccgagtatgt tccagcaaag cgggaatatc gctagccaca
      901 aaatattcgc cgtcgccaat cccaatcacc gaaggtggac ccagtcgtgc cgccacgatt
      961 ttcattggct ccttggcgga caaaaacacc aaagcgtaaa tgccgcgcag ttccttaaga
     1021 gacttgcgaa ccgcttcttc caatggcgta ttgttggcag tatgtttttc caccaaatgc
     1081 gcgacgattt ccgtatccgt ttcggtcacg aatttgtggc cttcgcgctg cagttgttct
     1141 ttcaattcca gataattttc gatgatcccg ttgtgaacga ccacgatctg cccgctgcaa
     1201 tcgcggtgcg gatgggcgtt ttcttccgtg ggacgtccat gggtcgccca acgcgtgtgc
     1261 ccaatcccat aagttccttc aatgggcgac ttggcgatcg cttcttccag gttgcgtaat
     1321 tttcctgcgg cacgcctgat ttccaacttg ccatctttgg tgactaccgc gattcctgcc
     1381 gagtcatagc cgcggtattc caatttccgc aacccctcga tgatcaccgg aacaaccttt
     1441 ttaggaccga tatacccgac aatgccgcac atgaaagagg ctcctattga tacttcaagg
     1501 ttaacatggg gaaaaaattg cctgcaaaat ccaacagaac atgtcgcgaa tgaggttatg
     1561 cgggttgcgg caacgctttc agaacctatg actttgtgat tcagtcgatg atttcgaacc
     1621 ggtattcttc gatgaccggg ttggttagta catccttcga gatccgctca agctggcctt
     1681 gagcctcctc ctgcgtccaa cccgcgagat ccaacacaaa aaatttcccc tgccgcacat
     1741 ccttcacctg gtcataaccc aatccagcga gtgcgtgttg aatcgtttgc ccttggggat
     1801 cgagcacggt gcgttttggc ataacccaga cgtgtgcttt cattcgacga ttataacatt
     1861 taggtctatg acggaaccga cctaggttgc gcgaaatctc agaatgccga tgtgcttttc
     1921 ccaaggccct gcagcgccac cttggcgctc tcatcaatga atgcgtgctc gaagctcatt
     1981 ttcgccatac gcgtcaattc cgcttcctgc aatcccatgc gagccgcgtt ttcatattcg
     2041 gatcgcagat tggtgtgaaa cattgctgga tcatcagtag aaagcaccac tggaattcca
     2101 tgccgaaaaa actgcggcag cgggtgctgc tcgatttgtg ccgcatcaac gcctagaagt
     2161 ttcgctagcg cccccgtcct gatgttgctc tgtgggcaaa tctccagagg gatgtgtcgc
     2221 tccgccagta aatccatcaa agacgggtcc cgaacggccg ctattccgtg gccgatgcgc
     2281 tccgccccaa gcagctcgat cgcttcgcga atttcttccg gcccgccgac ttcgccggcg
     2341 tgcatgagtt tgcgcaaccc gacggccccg gccttttcat acacgggccg gaattccttg
     2401 gccggcacac tcaattcgtc gcccccgatc ccgaacgcca caatcgattt tgaagcgcat
     2461 cgttccgcgg cctcgacaac tttcatggcc gcgtcggttc cgaattggcg cacggcatcg
     2521 aacacaaagt taagttgcag tccgcggcta cgaaaagact ccgcggccgc cacgatagct
     2581 tcgaagttct tttctgccga ttggtgccgt aaaagcatga ccccgatgga cagagtcact
     2641 tcggcgtaaa cgacattttg cgtcagcaac tgttccccaa gatcgcgcag cgccaaggtg
     2701 aagtcctccg gagcgcgaag gagcgaggaa acccatttga agcattcaat aaattccaga
     2761 aaatcgccgt atgcgtagcg ttccagaact tcttgcttgg atgccggcac tccatacttt
     2821 gcggccaaag cgcagaccgt ttctggttgc agcgaaccct ccaggtgcaa gtgcagctca
     2881 gcctttggca gcgccagaaa atcagcggaa agggaattgg attgtgctcc gaggttcaat
     2941 ggggaagttg cgggcgaccc gcgttaatcc aggcggttag ccagtaggac cctacatccg
     3001 tcgccgcatc ggagagctgt cgtatcaaaa tcaccgctgc cgcgttatag aaatgatcga
     3061 agtactcatc attataagaa ttttcgccac gtttggaatt tcggtccgcc agcaaaattg
     3121 tttccagcca actgtgcgag ttgaggcacg cctcaaaggc gtggtccgtt gggtcactga
     3181 tgtacgccgc gtcgtttggg cgcatgggaa aaaacgacga gtagcggtct atcaaagtag
     3241 tgccgaaccg ttcattgata ccgatttggg cagtgagatg gccgtcgaaa tcttcagtgg
     3301 tattgaaagg gtcgtgggct tccgccacat aattcgcaag gatcgcagcg tcgagtttgg
     3361 cttcgtccca atgcccggcc ttcatgtctt ccgttaattt ctgactgtag acgccgatct
     3421 gccaaggcag caagccgttg gattgaagct tggtttttcc atatttattg actgctgctt
     3481 tgtagtttcg cggcagcgcg tcgaaaggga accgtccgta tttatcgagg tacagaaaat
     3541 gattgtgacg ctccgctgcc ttggtggcga tggtatcgag cgggtcggtg acatgttgag
     3601 acaaaagcgc gcggttggac tcgaagaaag ctcgagtgtc tgccggtaag gtttcgatcg
     3661 ccttattaac caccaacttg ttgccgttac ggccccaggc ccgggcgctg tgcggtagga
     3721 ctacaaagat catcgctacg ctgatgaacc gcgtgcgacg aaatgttgtt ctatggcgcg
     3781 ccagtgacat gacggtaggt ctcagtctgc cttgctgctt actcttatgg ccgggccaca
     3841 gctcgcaagg tgctgcgctt ccgcccgtag ttgtagtaaa tcacaagtcc gataatcatc
     3901 cacacaaaga aacgaatcca gttggtgatg ggcaggccag ccatcaatag caggcacgtc
     3961 aagatcgtaa ggatcggcgc gatcgggcct cctggcgcgc gaaatgcccg ccgccgctcc
     4021 ggttcgcgat aacgcagaat caaaactccc cccgccacca gcgcgaacgc aaacaaagtt
     4081 ccaatgttcg agaggtccgc cagcgttcca atatccaaca aacccgctgg aatgccgacg
     4141 acgatacccg ccatcaacgt tgaaaaatcc ggagtctgaa atcgcggatg cacccggctg
     4201 aagatacgcg gcaacaatcc atcgcgagac atggcgaacc acacacgcgc ttgccccaat
     4261 tgaaaaacga gcagggaaga aatcatgccg aggagtgcgc cgatcaacac taccaaccgc
     4321 actccagtga aatgcaactt tttcaaagtg tttactaccg gagctgcgtc atcgagcagc
     4381 gtctgccacg gcaccaatcc cgtcagcacc accaccacac cgatataaag cagcgtgcac
     4441 acgatcagcg tcgcaataat tccaatcggc aagtcacgct gcggccgctt gcactcctct
     4501 gcagctgtcg aaaccgaatc gaatccaata tacgtaaaaa aaacaatcga gccacccgtc
     4561 aaaatacctg gccatccatt aggtgcgaaa gggtgccagt tagcggggtg cactagtttt
     4621 gacgcaaagc cgatgaacac cagaatggcg gtgatcttta gcaacaccat gatgttgttt
     4681 gcgccggccg attcgcgaat gccgcgaatt aaaattaccg taagcagcat cacaatcagg
     4741 aatgccggaa agttaaatcc aaagtgccac cccggatcaa gcagcatgtt cccttgaaag
     4801 tctgacattc cagttggcag gtaagccggc gagatccacc tgggattcgg atggatgcca
     4861 aaaaaatcaa gcaggtccac aatgtgggca gaaaatccca cacaaactgc catattgctg
     4921 accgcatatt ccagaatcaa atcccaaccg ataatccacg caattaactc gcccatagtg
     4981 gcgtacgtgt atgtatacgc gctcccggcg atgggaatca tcgctgccag ttccgcgtaa
     5041 cataccgacg caaacccgca aacaatcgcc accagcacga acgacagcgc aatcgccgga
     5101 cccgcaccag gccgtccgaa cgacgccgaa tggtaaatca aatattcaag aaggggagcg
     5161 ttcaggatcg aagaagcttg caggtgctgc ccggcgatcg ccgtaccaat caccgtgaaa
     5221 attcccgagc cgataaccgc gccgatgccc agcgccgtca aacttaccca accaagggat
     5281 tttttcaggt ggacttcggc attctcagaa tctgacaaca gttgatcgat acccttgcag
     5341 cggaagagat ctgacgccac gcacacactc caatcgctct tgggttcccc gggctcgact
     5401 ggatcaaatt gaatccaatt cgcgcaacga ggatggaagg ctgtttgcgc ctcaatgtac
     5461 cttgccaagc cag
//
LOCUS       DATCBB010000005         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_64922, whole genome shotgun sequence.
ACCESSION   DATCBB010000005 DATCBB010000000
VERSION     DATCBB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64922"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VII37_00050"
     CDS             <1..910
                     /locus_tag="VII37_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5068134.1"
                     /translation="FFLLSAPFAVIVLLAPVRTGAFAAGPHATEAGAGLGLLGGVLVA
                     MWNYMGWDNASTIAQEVERPQRTYPKAMIAAVILVSLTYVLPFAAVYFMGVPATAFAE
                     DGSWAMVAGLVGGKIMGFEWLRFLVVLGGMMSAFGMFNALVMSYSRLPLAMARDGMLP
                     KIFANTSARTQAPWVAIIACATCWALCLGLGFKRLVTLDIMLYGAALMLEFVTLVALR
                     IREPQLKREFRVPGGLAGAILAGVFPLALLMIALVQSESQSVLGINGLVFGVLIMAAG
                     FALYASTRKLRKRLPAEVNAEIAEPA"
     gene            1050..>2400
                     /locus_tag="VII37_00055"
     CDS             1050..>2400
                     /locus_tag="VII37_00055"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068135.1"
                     /translation="MKIPCLKRRILTYLSIVTATFSALPSSPAQEHSTPSRIDEKFFQ
                     ELHWRSIGPHRGGRALAVTGVRGQPELFYFGSVDGGVWRSNDAGRTWKPIFDSQPIGS
                     IGAIAVAPSNPNVIYVGSGEADMRSNIGNGDGIYKSTDAGKSWTHIGLTDSRQIARIL
                     VDPADSNKVFVAALGHAYGPNAERGVFRSKDGGATWQRILFHDENTGAIDLAFGPGNT
                     NLIYAALLQTRRPPWNVYPPSKGPGSGLYRSSDGGDHWEAVNGRGLPAEEVGRIGIAF
                     APSNPQRIYLIVDAKAGGLYRSDDSGKNWQLMSQDERIWQRGWYFGEVSVDPKDADTV
                     YIPNTTVYRSRDGGKTFTAFKGAPGGDDYHQLWIDPEEPRRMILGSDQGTIVTRNGGE
                     TWSSWYNQPTGQMYHVSTDNRFPYWVYGAQQDSGSIATPSRGNYRTLDFHDWRPIEAG
                     "
BASE COUNT          510 a          630 c          703 g          557 t
ORIGIN      
        1 gttctttttg ctttccgctc cgttcgcggt gattgtgtta ctggctccgg tgcgaactgg
       61 cgcgtttgca gctggaccgc acgccaccga agctggcgcc gggctcggtt tacttggggg
      121 agtgttagtg gccatgtgga attacatggg ctgggataat gcctccacca tcgcgcagga
      181 agtcgaaagg ccccagcgca cctacccaaa ggcgatgatt gccgcggtta ttctggtttc
      241 gctcacgtac gtgcttccat ttgccgcggt ttattttatg ggtgtccccg ccacggcctt
      301 cgcagaagac ggatcatggg cgatggtcgc cggcctcgtg ggcgggaaaa taatgggatt
      361 cgaatggcta cgcttcctgg tggtgctcgg cggcatgatg agcgccttcg gaatgttcaa
      421 tgcgttggtg atgagctatt cgcgattacc tctggcgatg gcgcgcgatg ggatgctgcc
      481 caagattttc gcaaacacca gcgctagaac ccaggcgccc tgggtggcca tcattgcgtg
      541 cgctacttgc tgggctctgt gcctggggct agggttcaaa cgattggtga cactcgacat
      601 catgctttac ggcgccgctt tgatgcttga gtttgtgacc ctggtggcac tgcgtattcg
      661 tgagccgcaa ttgaagcgcg agtttcgagt tcccggcggg ttagccggag cgatcctcgc
      721 gggtgtattc cccttggcgt tgctgatgat cgccctagtg cagagtgaaa gccagtcagt
      781 gctgggaata aacgggctgg tttttggtgt gctgattatg gccgcgggat tcgcgctgta
      841 tgcctcgacg cgcaagcttc gcaagcgact gccagcggaa gttaatgcgg aaattgcgga
      901 accagcgtga tggctcttct ctctaggact accttcatcg actgcacagc ttaaaattct
      961 ttgcgtgcaa aacttcaagg attggccctt cgtccgctga gatatacttc cggctcgttc
     1021 aactagaatt tgccttcgga gaatagcccg tgaaaattcc gtgcttgaag cggcggatac
     1081 tcacatatct gtccattgtt acagcgacct tctctgcatt gccaagctcg cctgctcaag
     1141 agcacagcac cccgtcacgc atcgacgaaa aattttttca ggaactgcac tggcgctcca
     1201 tcggaccgca tcgcggtgga cgcgcgctcg ccgttactgg tgtacgagga cagccggaat
     1261 tattttattt tggctccgtg gacggcggcg tatggcgctc gaatgacgcg gggcgaacat
     1321 ggaaacctat tttcgattct cagccgattg gttccatcgg ggcaattgcc gtcgcgcctt
     1381 caaatcctaa tgtgatctac gttggatcgg gcgaagccga tatgcgctca aatattggca
     1441 atggggacgg catatataag tctactgacg caggcaaatc ctggacacat attggattga
     1501 ctgactcccg ccagattgcg cggatcctgg tggatcctgc agattccaat aaagtatttg
     1561 tcgctgcgct agggcacgct tacggaccta acgccgagcg aggcgtgttt cgatccaaag
     1621 acggaggggc gacgtggcag cgcatacttt ttcacgatga aaacaccggc gccatcgatc
     1681 tagcattcgg gcccggaaac acaaacttga tttacgccgc actgctccaa acccgacgcc
     1741 caccctggaa tgtgtatcca ccttccaagg gtcccggcag cggcttgtac cgttccagcg
     1801 acggtggcga tcattgggaa gcagtgaacg gccgaggatt acccgcggaa gaagttggcc
     1861 gcatcggaat tgctttcgca ccaagtaatc cacagcggat ttatcttatt gttgacgcca
     1921 aagcgggggg actttatcgc tcggatgaca gcggaaaaaa ttggcagttg atgtcgcaag
     1981 atgagcgcat atggcaacgc ggatggtatt tcggtgaagt tagtgtcgat cctaaagatg
     2041 cagataccgt gtacatcccg aatacaacgg tgtatcgctc acgcgatggc ggaaaaacat
     2101 tcacggcgtt caaaggtgct ccgggcggcg acgactacca tcagctgtgg atcgacccgg
     2161 aggaaccgag acgaatgatc ttgggaagcg atcaagggac tattgtcaca cgcaacggcg
     2221 gtgaaacctg gagctcctgg tacaaccaac cgaccgggca gatgtatcac gtcagcaccg
     2281 acaatcgatt cccgtattgg gtttatggcg cgcagcaaga tagcggctcc atcgcgactc
     2341 ctagccgggg aaattatcgc acgttggatt ttcacgactg gcgaccaatc gaagcggggg
//
LOCUS       DATCBB010000006         1366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_454276, whole genome shotgun sequence.
ACCESSION   DATCBB010000006 DATCBB010000000
VERSION     DATCBB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454276"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VII37_00060"
     CDS             complement(<1..243)
                     /locus_tag="VII37_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068136.1"
                     /translation="MMETEAFREFKTGLNLLRDNYAEKALPHMRRAVDLDRNNPYYMS
                     YLGVVLARSERKWGEAERLCDSAVRMKRNQAQLYLNL"
     gene            complement(578..>1366)
                     /locus_tag="VII37_00065"
     CDS             complement(578..>1366)
                     /locus_tag="VII37_00065"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY5068137.1"
                     /translation="KMIVAGASAYSRIIDFERMSKIAKAAGALFFVDMAHIAGLVAAG
                     FHPSPVPHADFVSTTTHKTLRGPRGGMVLCKTAFAKELDKLTFPGTQGGPLVHIISAK
                     AVCLKEAMEPSFKEYQKQVALNAKAMAAAIAKRGFRIVTGGTDNHLFLVEVHSRGITG
                     TDAEKALDRAGITVNKNSIPFDPLPPMKGGGIRLGSPSVTTRGLREPEMQQVGDWVAD
                     VLEHIGDATVEQRVRKQVAELAGRFPVYAARMNIHAASRAQVTA"
BASE COUNT          302 a          392 c          350 g          322 t
ORIGIN      
        1 gagattcaga tataactgcg cctggttgcg cttcattcgc accgcggaat cgcacaacct
       61 ttcggcttct ccccattttc tttcagatcg cgccaggact accccgagat aggacatgta
      121 gtatggattg ttacggtcga ggtccacggc acggcgcata tgcggcaaag ctttttccgc
      181 ataattgtcg cgcagcaggt tcagcccggt cttgaattcc cggaacgctt cggtctccat
      241 catagattcc cctcgttagt gatatttgct gcgaattact ggaaatttct gcgcgcagca
      301 agcttcaaag ccaccctcag agcaggcttt tcacttattg cgacaggcaa aacctgaacg
      361 gtcttaacat gaataaacaa acgggtgagg acgaaaagac cgagcagagt tgcataaaag
      421 attcccatta ggattgtgcg agattactcg cctgatattt tggaagctac tggccgaacg
      481 gccagtcttg ggacagccga actagtttac taaggagttg ttctggaaat tactgcgacc
      541 gcgctttgag aggcgatcac tggctttttt tgcaatatca ggcggtcact tgcgcgcgac
      601 tggcggcatg aatgttcatg cgcgccgcat acacaggaaa acgtcccgcg agttcggcca
      661 cttgtttacg tacacgttgt tccacagtgg catcaccgat gtgctcgagc acgtcagcga
      721 cccagtcgcc gacctgctgc atctccggtt cccgcaaccc tcgcgtggtc actgaaggac
      781 tgcccagccg tattcctcca cctttcatcg gcggcaatgg atcaaacgga atggagtttt
      841 tattgacggt aattcccgcg cgatccaatg ctttctccgc atccgtaccg gtaatcccgc
      901 gcgaatgcac ttccaccaga aataaatgat tgtccgtgcc accggtaaca atgcgaaaac
      961 ctcgtttggc gatggctgcg gccatggcct tggcgttcag cgccacctgc ttctgatact
     1021 ctttgaagga aggttccatc gcttccttca aacacaccgc tttggcggaa atgatgtgca
     1081 ccagcggccc gccctgggtc ccgggaaacg tcaatttatc caattccttc gcgaatgctg
     1141 tcttgcaaag caccattccg ccgcgcggcc ctcgcaacgt tttatgcgtg gtggtggaga
     1201 caaaatccgc gtgcgggacc gggctcggat ggaagcccgc ggcaaccagt ccggcgatgt
     1261 gcgccatatc cacaaaaaac agcgcgcccg cagccttggc gattttgctc atgcgctcga
     1321 aatcgatgat gcgcgagtac gcgctggctc ccgcgacgat catttt
//
LOCUS       DATCBB010000007         3583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_65003, whole genome shotgun sequence.
ACCESSION   DATCBB010000007 DATCBB010000000
VERSION     DATCBB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65003"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VII37_00070"
     CDS             <1..393
                     /locus_tag="VII37_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5068138.1"
                     /translation="AEATVPKITVITRKAYGGAYCVMASKHLRTDANFAWPTAEIAVM
                     GPEGAVNIVYKRELDKASESNREILRQEKIEEFRERFANPFVAAERGYIDAVIEPAET
                     RTRIITALRALENKRDTNPRKKHGNIPL"
     gene            complement(448..2391)
                     /locus_tag="VII37_00075"
     CDS             complement(448..2391)
                     /locus_tag="VII37_00075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5068139.1"
                     /translation="MSKRILMMVAAILLAGVAEASAQDAVVAPAENLVVDGVPKIPAS
                     LAETAGRYGSYRFAVVSDWHPTRREMLIRTRFADTPQVHLVKMPGGERQQLTFYPDSI
                     AEAQFHPNGGDYFVFMKDIGGGEWFQLYRYDMDNGNVTLLTDGKSRNLLGSWSSGGDQ
                     IAYVSTRRTGQDTDLWVMNPANPKSDHLLTQLKGGGWEPIDWSADDKNILLQEQISIN
                     ETYLWLVNTATGEKTAITPRDTKEKVSYGSARFTKDGKGIYVTTDQDSEFQRLALLDM
                     SGKNPKFLTSDIPWDVESFDLSRDGKRLAFVTNEEGLSVLHVMDTATQKEVKLPKLPA
                     GVMQGLRWHRDGHDLAFSLTNAQGAGDCYSLDVASNKLERWTMSETAVKTDAFPAAEL
                     VHWKSFDGKMISGFLYKPPAKFTGKRPVLVMIHGGPEGQSQPVFLGRANYFLNELGIA
                     LIYPNVRGSTGYGKTFSLMDNGFKREDTYKDISALFDWVGSRADLDADKIAVTGGSYG
                     GHMTLAVSTFYSDRIRCSVDVVGMSNLVTFLEHTEGYRRDLRRVEYGDERDPKMHEFL
                     EKIAPMNNIEKIKKPMLVVAGKNDPRVPVTESEQIVGALKKQGTPVWYLMAKDEGHGF
                     RKKQNQDFQFYATVEFLEEYLLK"
     gene            2504..>3583
                     /locus_tag="VII37_00080"
     CDS             2504..>3583
                     /locus_tag="VII37_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5068140.1"
                     /translation="MFRKILIANRGEIAVRILRACRELGIRSAAVFSEVDRKSLHVRL
                     ADEAYPIGPAASAESYLRIDKLMDVARRAGCDALHPGYGFLAENAALPRACADAGVTF
                     IGPSAEAMEALGSKTAGRQLARRSDVPSVPGTNDPIQTLQQAQALIENIGLPVLLKAV
                     AGGGGKGMRVVNRLSDLAAAFRDASSEAQNSFGDGRLYLEKYLLKPRHVEIQILADSH
                     GRVVSLGERECSVQRRHQKVIEEAPSPIMTPDLRRKMGDAAVRLARAGGYTNAGTVEF
                     LVDANLDFYFLEVNTRLQVEHPVTEQVTGLDLVKLQIAIAAGHRLPFAWETITPRGHA
                     MEVRLYAEDPDNNFFPSPGKILSRHA"
BASE COUNT          822 a         1073 c          874 g          814 t
ORIGIN      
        1 gccgaagcta ccgtgccaaa gatcacggtg attacccgca aggcttacgg cggtgcatat
       61 tgcgtgatgg ccagcaaaca cctgcgcacc gacgcaaact tcgcgtggcc aaccgctgaa
      121 atcgcggtta tgggtcccga aggcgcggtc aacatcgtct ataaacgtga actggataaa
      181 gcttcggaat caaaccgcga gatactgcgc caggaaaaaa tcgaggaatt cagggagcgc
      241 ttcgccaacc cattcgtggc tgccgaacgc ggctacatcg atgccgtcat agagcccgcg
      301 gaaaccagga cgcgcatcat cactgccttg cgagcattgg aaaacaagcg cgataccaat
      361 ccgcgcaaga aacacggcaa cattccactg tagctaagtc gctgctctgg caacgaaagt
      421 caaaggtcgg ccttcaatgc aacttcgtca cttgagaagg tattcctcta aaaactccac
      481 tgtcgcgtaa aactgaaaat cctgattctg cttcttgcga aagccatgcc cttcgtcttt
      541 ggccatcaaa taccacactg gagtcccctg tttttttaac gcgcctacta tttgttcgga
      601 ttcggtcact ggcacgcgcg gatcgttctt cccggccacc accagcatgg gtttcttgat
      661 tttctcaatg ttattcatgg gcgcaatttt ttctagaaat tcgtgcattt tcgggtcacg
      721 ctcgtcgccg tattccacgc ggcgtaaatc gcggcgatag ccttcggtgt gctccaaaaa
      781 tgtaactaaa ttggacattc ccacaacgtc taccgagcac cgtatgcgat cgctataaaa
      841 agtggaaact gccagcgtca tgtggccacc gtagcttccg cccgtcacgg cgattttgtc
      901 ggcatcgaga tccgctcggc tccccaccca gtcgaagagg gcgctgatgt ctttgtaagt
      961 gtcctcacgc ttgaatccgt tatccatcaa tgaaaaagtc ttgccataac ccgtcgaccc
     1021 tcgtacgttc ggataaatta aggcgatgcc cagctcgttc aaaaaataat tcgcacgccc
     1081 caggaaaacc ggctgagact ggccttccgg accgccatga atcatcacca gcactggtct
     1141 cttgccggta aactttgcag gcggcttgta caaaaatcct gaaatcattt ttccatcaaa
     1201 gctcttccag tgcaccaact ccgccgccgg aaatgcatcc gtctttaccg ccgtttcgct
     1261 catcgtccag cgctccagct tgttactcgc gacatccaac gaatagcaat ctcccgcccc
     1321 ttgagcattc gtgagtgaaa aggccaaatc atgcccatcg cgatgccaac ggagcccctg
     1381 cattaccccg gcgggcagtt ttggcaattt cacttctttc tgcgtcgctg tatccatcac
     1441 gtgcaaaaca ctgaggcctt cttcgttggt gacaaaagcc aaccgctttc catcgcggga
     1501 aagatcgaag gactccacat cccagggaat gtcgctagtc aaaaacttcg gattctttcc
     1561 agacatatcc aggagcgcca gtcgctgaaa ttccgaatcc tggtcagtcg tcacataaat
     1621 tcctttgcca tctttagtga atcgcgctga gccgtaggac actttttctt tggtatcgcg
     1681 aggcgttatg gcggtcttct cgccagtcgc ggtgttcacc agccacagat atgtttcgtt
     1741 gatagaaatc tgctcttgca gcaaaatatt tttatcgtca gcagaccagt caatgggctc
     1801 ccaaccgccg cctttcagct gtgttaacaa atgatcgctc ttcggattgg caggattcat
     1861 cacccataaa tctgtatcct gtccggtgcg cctggtggaa acataggcaa tctggtcacc
     1921 gccggaagac cacgagccca gcaaattccg cgatttgcca tcggtcagca gcgtgacatt
     1981 cccgttatcc atatcgtacc ggtacagctg aaaccattcc ccgccgccaa tatctttcat
     2041 aaaaacaaaa tagtcgccgc cattggggtg aaactgcgcc tccgctatcg aatcgggata
     2101 aaacgtcaat tgctgccgtt cgcctccggg cattttcacc aaatgcactt gcggggtatc
     2161 cgcaaaacgc gtgcggatca gcatctcgcg gcgcgtagga tgccaatccg aaactaccgc
     2221 gaagcggtaa gatccgtaac gcccggcggt ctctgccagc gaagccggaa ttttgggcac
     2281 gccatcgacc accaggttct ccgcgggagc aaccaccgcg tcctgtgcgc ttgcctccgc
     2341 aacgccagcc aataaaatcg cggccaccat catcaaaatt cgcttgctca ccccaacctc
     2401 cttgaatcac tcattcgtct agaaacgatt ggacacctta ctccaacgcg gggctttggc
     2461 caagcgcgcc gcgtgttaaa atttcggcga accaccgcca gccatgttca gaaaaattct
     2521 gatcgccaat cgcggagaaa ttgccgtgcg catcctgcgc gcttgccgag agctgggcat
     2581 ccgctctgcc gcggtattca gcgaggtgga tcgtaaatcc ctgcatgtcc ggttggccga
     2641 tgaagcttac ccgataggtc cggcggcgtc agccgagagc tatcttcgta tcgataaact
     2701 gatggatgtt gcgcgccgcg cgggctgcga cgctttgcat ccgggttacg gttttctggc
     2761 ggaaaatgcc gcgcttcctc gcgcctgcgc agacgccgga gtcaccttta tagggccgtc
     2821 cgcggaagcc atggaagcgc ttggcagcaa aacggccggc cgccagctcg cccgacgctc
     2881 cgatgtcccc agcgttcccg gtacaaacga ccccatacaa actctgcagc aggcccaagc
     2941 cttaatcgaa aatattggct tgcctgtttt gcttaaagcc gtcgctggtg gtggcggaaa
     3001 gggtatgcgg gttgtaaacc gccttagcga tctggcagcc gctttccgcg atgcgtcctc
     3061 ggaagcccag aatagctttg gcgacgggcg cctttatctt gaaaagtatt tgctcaagcc
     3121 acgtcacgtg gaaattcaaa ttctcgccga ttctcacggc cgtgtcgttt cgctcggcga
     3181 acgcgaatgc tccgtacagc gccgccatca aaaagttatt gaagaagctc cttcgccgat
     3241 catgaccccc gatctacgca gaaaaatggg tgacgccgcg gtgcgtctcg cgcgcgccgg
     3301 tgggtatacc aatgcgggca cggttgaatt tctcgtcgat gccaacctcg atttttattt
     3361 tctggaagta aacactcgct tgcaggttga gcaccccgtt accgagcaag taaccgggct
     3421 ggatctcgtc aaattgcaga tcgccatagc cgccggacat cgccttccat tcgcgtggga
     3481 aaccatcact ccgcgcggtc atgccatgga agttcggctg tacgccgagg atccagacaa
     3541 caatttcttt ccctcaccgg gaaaaattct ttctcgccac gcg
//
LOCUS       DATCBB010000008         1027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_357020, whole genome shotgun sequence.
ACCESSION   DATCBB010000008 DATCBB010000000
VERSION     DATCBB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357020"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..>1027
                     /locus_tag="VII37_00085"
     CDS             384..>1027
                     /locus_tag="VII37_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5068141.1"
                     /translation="MWIVALALRRPYTFVVMALLIIILSPIVIFRTPTDIFPDINIPV
                     ISVVWNYAGLSPQDMANRITTNTERGLTTLVNDIEHIESQSLNGVAVVKIFFRPTANI
                     PTALAQVTAISQTALRFLPPGANAPLVIQYSASSVPILQIGLSSKTLPEQELNDIALN
                     FLRTQLITVPGAAIPYPFGGKTRVVSVDLDTPALQSKHLSAVDVVNAISAQNLI"
BASE COUNT          218 a          268 c          267 g          274 t
ORIGIN      
        1 catgggctgc cgcgatcagt tcttcaggat tggtgccggg ggcgttctcg aacctcgtgg
       61 taaaagagta gggagtgtgt gataaaacac cgcttggcgc cgacacttcc cctttgccat
      121 ccttaagtcc gccggtccaa actgcactcg cgttacgctg cattcgggaa tctcctcgat
      181 tgagtagtca cggtttcatt gtagatgcgc gcggggagtt gcgccagatt ggcggtgaga
      241 tgtgtcaaac atttagaaag cgcggcaaac ttccgcctct cagtattact tttgtggctt
      301 ttaaatctat aagcgtttag atttcatcta agttagttct gatccggcct tgtacgggat
      361 ctaattaagg caaaaaatac tccatgtgga ttgttgcgtt agccctgcgc cggccataca
      421 cgtttgtggt catggcgctg ctcataatta ttttgagccc cattgttatt tttcgcaccc
      481 ccactgacat ttttcctgac atcaatattc cggtcattag cgtggtatgg aactatgcgg
      541 gattgtcgcc gcaggatatg gcgaatcgca tcaccacaaa cacggagcgc ggattaacca
      601 cgctggtgaa cgatatcgag catattgaat cgcagtctct caacggcgtt gccgtagtta
      661 agatattttt ccgtcccacg gccaatattc cgaccgcgct tgcccaggtt acggccattt
      721 cacagacggc gcttcgcttt ttgcccccgg gtgcgaacgc accactggtc attcaatact
      781 cggcttcgag cgtgccgatt ctgcaaattg gcctgagcag caagacgctg cctgagcagg
      841 aactgaacga tatcgcgttg aattttctgc gcacgcagct cattacggtg ccgggtgcgg
      901 ccattccgta cccgttcggc ggtaaaacgc gcgtggtttc ggtggatttg gacacccccg
      961 cattgcagtc caaacatctc agcgcggttg atgtggtgaa cgcgatcagc gcgcagaatt
     1021 taatttt
//
LOCUS       DATCBB010000009         3223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_421964, whole genome shotgun sequence.
ACCESSION   DATCBB010000009 DATCBB010000000
VERSION     DATCBB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421964"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VII37_00090"
     CDS             complement(<1..1072)
                     /locus_tag="VII37_00090"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5068142.1"
                     /translation="MRRNLFAKLGLTFVALLISALLAVDYFAERALRRDYERTAYSQL
                     TAVAGIAQARASALPALPPARPEDVARLNEWVSQIAASGSRVTLIASDGQVLAESQSD
                     SQTMENHAGRPEVREALAKGSGRAIRHSVTLNRDLLYYAVRHDMATGPPIIMRFALPI
                     ETVDEVLQSFRRSLWLSSFLILLIAAVAALQISRVFTDRVERLKDFSRRVAAGDFTAI
                     AADSSGDALDDLGASMNRTAAQLDETIRTLTEERNLSTAILGSMVEGVAVVNGSERLL
                     FANQGFAEILNLKFPPKSGSALVEIVGQTELIEAVRSVLHGESRVEAEIVTGTLRQKY
                     FAATVASVRAGDTLGAVVVLHDI"
     gene            complement(1062..1862)
                     /locus_tag="VII37_00095"
     CDS             complement(1062..1862)
                     /locus_tag="VII37_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5068143.1"
                     /translation="MSIEIPSHHDPAHVYRNAPLYDTLPPRHRKARSISMKRILIIED
                     DRDIVELVRYNLANEGFQVSAASDGNSGLNSLKKTPPDLLLLDLMLPKLSGLDICREI
                     RRDDSLNRLPILMLTARGDEADRVVGLEMGADDYVTKPFSPRELIARVKALLRRAEPP
                     TDSPRLIEIGKLAIDPASYRVSHSGKAVPLSTLEFRLLYYLASRPNRVFTRDQLLDAV
                     WGTDRFVTPRSVDVYVRRLREKIEPDPENPMHLKTVRGAGYLFETRAA"
     gene            complement(1967..>3223)
                     /locus_tag="VII37_00100"
     CDS             complement(1967..>3223)
                     /locus_tag="VII37_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068144.1"
                     /translation="ELKKLPHLRRLPDYWSPYKRGRYDAALEVDTGVPLEKLAEITDA
                     LCRVPQGFHVHPKIVKLLEQRVEMGHGKRAVDYGFAEALAFGSILLEGNPVRLTGQDT
                     QRGTFNQRHAVLVDTQTEEEYLSLAHLSPEGAPDRPFCEIANSPLSEAACVGFEYGFS
                     RDYPEALVAWEAQFGDFVNGAQVIIDQFISAGEDKWDLPSGLVMLLPHGYEGQGPEHS
                     SARIERFLQLAGEDNIQICQPSTAAQYFHMLRRQVRRPWRKPLIVFTPKSMLRHPDAS
                     SPIQDFAEPRFLPLVPDREIQDAKRILIASGKVGHELRAERKRRNDTETAIFFLEQFY
                     PLPRLDIAAAITAHPNAREVIWVQEEPRNMGGFFYVVPRLESLAKGLGLRLRSVKRSS
                     SASPATGSAKAHELEQKTLLALAFTT"
BASE COUNT          716 a          928 c          895 g          684 t
ORIGIN      
        1 tgatgtcgtg cagcaccact actgcgccga gagtatcgcc tgcccgcacg gacgctactg
       61 tcgcggcgaa atacttttga cgcagtgtcc cagtgacaat ttctgcctcc acccgcgatt
      121 ccccatgcaa tacactacgc accgcctcaa tgagttccgt ctggccaaca atttccacca
      181 gcgcgctccc cgatttcggc ggaaacttca ggttaagtat ttctgcaaat ccctgattgg
      241 cgaaaagtag gcgttcagat ccgttcacga ccgcaacgcc ctccaccatg cttcccagaa
      301 tagccgtgga gagatttcgt tcttccgtca acgtgcgaat ggtctcgtcg agttgcgccg
      361 cggtgcgatt cattgaagcg cctaaatcgt caagggcgtc gccggaactg tcggccgcaa
      421 tggccgtgaa gtcaccggca gccacgcgtc gagaaaagtc tttcaagcgc tcgacgcgat
      481 ccgtgaagac ccgggaaatc tgtaaagctg ccactgcggc aatgagcaaa atcaaaaatg
      541 aggaaagcca caggcttcgg cggaacgatt gcagcacctc gtccacggtt tcaatcggca
      601 gagcgaagcg cataataatc ggtggcccgg ttgccatgtc gtgccgaacc gcgtagtaca
      661 gcaggtcgcg gttcagagtg acgctgtgac gaatggcgcg cccgctgccc ttcgccagtg
      721 cttcgcgaac ttccggacga ccggcgtgat tctccatcgt ctgtgaatca gactgcgact
      781 cagccagtac ctgtccgtcg ctagcaatca atgttacgcg cgacccgctt gccgctattt
      841 gcgaaaccca ttcattcagc ctggcgacat cttccggccg agcgggcggc aacgctggca
      901 gggccgatgc gcgggcctgc gcgattccag cgacggcagt aagttgggaa taagcggtgc
      961 gttcgtaatc gcgacgcaga gcgcgttccg cgaagtaatc taccgctagc aaagcgctga
     1021 tgagcagcgc cacgaaggta aggccgagtt ttgcgaaaag gttacgccgc acgggtttcg
     1081 aacaagtacc ccgctccgcg caccgttttc aaatgcatcg gattttccgg gtctggttcg
     1141 atcttttcgc gcaggcggcg gacataaaca tcaacgcttc gaggggtgac gaaacgatcc
     1201 gttccccaga cagcgtcaag caattgatcc ctggtgaaca cgcgattcgg gcgcgaagca
     1261 agataataca acaaacgaaa ctcaagcgtg ctgagcggaa cggcttttcc agaatgactt
     1321 acgcgataag aagcggggtc aatcgccaat ttcccaattt caataaggcg cggcgaatcc
     1381 gtaggcggtt ccgcgcgacg caacagcgct ttcactcgcg caatcaattc gcgtgggctg
     1441 aatggcttgg tgacatagtc gtcagcgccc atctcgaggc ccacaacgcg atccgcctcg
     1501 tccccacgcg cggtaagcat gagaattggc aaacgattca gtgaatcatc gcggcgaatt
     1561 tctctgcaaa tatccaggcc cgaaagcttg ggcagcatca ggtccagaag cagcagatcg
     1621 ggcggagttt ttttcaggga gttcaagccg ctgtttccgt ccgaagcggc gctaacttgg
     1681 aagccttcat tggccaagtt gtagcgcacc agttcaacga tgtcgcggtc gtcctctatg
     1741 atcaggatgc gtttcatgct gatggagcgg gccttccggt gtctgggcgg cagtgtatcg
     1801 tacagcggcg cgttgcgata gacatgtgcg ggatcgtgat ggctaggaat ctcaatggac
     1861 acggctcatt gtggcaaagc ccgtatgaag tggatatgac gcccagatga acgtcgcgtg
     1921 aacgtttggt ttgcgctctg ggtgagaaag aattgcttca attaagttag gtagtaaaag
     1981 ccagcgccaa cagcgttttt tgctctagct cgtgtgcttt tgcggagccg gtggccgggc
     2041 tggcgctgct cgagcgcttc accgagcgca gtcgcaatcc caatcctttg gcaaggctct
     2101 cgagacgcgg caccacatag aagaacccgc ccatattccg cggctcttcc tgaacccata
     2161 taacttcgcg tgcgttgggg tgcgcggtga tcgcagcagc aatatccagt cgcggcaaag
     2221 gataaaactg ctccaggaaa aagatcgccg tctccgtatc atttcggcgc ttacgttccg
     2281 cgcgcaattc gtggccaact ttgccactgg cgatgagaat gcgctttgcg tcctgtattt
     2341 cgcggtctgg gactaacggc aaaaagcgcg gctcggcaaa atcctgaatc ggagaactgg
     2401 cgtcgggatg acgcagcatg ctcttgggcg taaacacgat cagcggtttg cgccacggcc
     2461 ggcgcacttg tcttcgcaac atgtgaaaat attgcgcggc cgtcgagggc tggcagatct
     2521 gaatattgtc ttcgccagcc agctgcaaaa atcgttcgat gcgtgcgctg gagtgctcag
     2581 gaccctgccc ctcgtaacca tgcggcagca gcatcaccaa tcccgaaggc aaatcccatt
     2641 tatcttcccc ggcactgatg aattggtcga tgatcacttg cgcgccattc acaaaatcgc
     2701 cgaattgtgc ttcccacgcc accagcgctt ccgggtaatc ccggctgaag ccgtattcaa
     2761 aaccgacgca cgcggcttcc gacaacggag aattagcgat ttcgcaaaaa gggcggtccg
     2821 gagccccttc cggcgaaaga tgcgcgagcg acagatattc ctcttcggtt tgtgtatcca
     2881 cgagaaccgc atgccgctga ttaaatgtgc cgcgctgcgt atcctgtccg gtcaggcgaa
     2941 ccggattccc ttccagcaaa atggatccga acgccagcgc ttccgcgaaa ccataatcca
     3001 ccgcgcgttt gccgtgcccc atctctacgc gctgctcaag cagtttcaca attttgggat
     3061 gaacgtgaaa gccttgcgga acgcggcaaa gcgcatcggt aatttctgcc agtttttcga
     3121 gaggcacacc ggtatcgact tctagcgcgg cgtcatagcg cccccgttta tacggtgacc
     3181 agtaatctgg aagtctgcgc aggtgcggta atttcttcag ttc
//
LOCUS       DATCBB010000010         6708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_259845, whole genome shotgun sequence.
ACCESSION   DATCBB010000010 DATCBB010000000
VERSION     DATCBB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259845"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VII37_00105"
     CDS             <1..973
                     /locus_tag="VII37_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068145.1"
                     /translation="AVRTYPLSSRKSKVNVADFAKPPARNVSISKFMDSLPNFLAAED
                     LRQLLAAVQLARSGKKTILWGIGGHVIKVGLGPILIDLIERGFVSGIAMNGAALIHDF
                     EIAIAGNTSEDVDAGIHAGQFGMARETGEQLNEIAKNAARSGTGYGEAAGQFLTSTAA
                     KARHASSSVVAAAYRRRIPVTVHLAIGADIPHMHPAADGAALGAVTYQDFRLFCALVK
                     SMHPGGVYMNWGSAVLLPEIFLKAVSVVRNLGVPLRPITTANFDFIQHYRPLQNVVKR
                     PTLSVSNRRGIESKGYAITGHHELLMPLVAAALASGWPKRGPKKNRT"
     gene            970..1920
                     /locus_tag="VII37_00110"
     CDS             970..1920
                     /locus_tag="VII37_00110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5068146.1"
                     /translation="MNDHDENFANGTPEDAVNPPTETPAQLAPPVTEPVPESQPSQVL
                     SGDPMAAAIPAFQAKRILPEDLRVSWSWPHLIIFIFFGFASLMIVQLGFVFYVAANRH
                     LSAVQVQRVFENSPQLLVETNVLWYALLFLFLYVTLAVLRDVPFWRSLGWRKLSANPA
                     RGTGSGWMYFFSGCGLAIFVALASYNMKDTDHLPIQELFKNRSGALLLMSMAVFVAPL
                     VEETVFRGYLYPLFAKSLGVIPGILITGVLFGLMHGSQLGWTFRLVLLLTVVGVIFTF
                     ARARTGTVMASFLLHLGYNSMIALTSIIATRGFQHMPVSP"
     gene            2022..3092
                     /gene="mtnA"
                     /locus_tag="VII37_00115"
     CDS             2022..3092
                     /gene="mtnA"
                     /locus_tag="VII37_00115"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY5068147.1"
                     /translation="MYVAQPIEWTEQGVVMLDQRRLPAEEISYTYTDYREVAKAIREM
                     IIRGAPAIGVAAAMGVALGVLHSTATSVDTLKSEFAEICDTLAKTRPTAVDLFWALER
                     MKRRFAELSATSKPLVEIKRAMIEEAKLVHLEKKATDQAIGEYGAEFMPADGQVMTQC
                     NAGALATGGIGTALGVIRVAYQQGKKLHVLVPETRPYLQGSRLTAWELHKGGIPLTLI
                     TDNMVGHFLKTGKVRAIVTGADRIAANGDTANKIGTYQIAVLAKENNVPFYIAAPVST
                     FDLSIPDGDHIPIEERAASEVTHLQGLRIAPDVHAAHPAFDVTPARYIAAIFTERGVA
                     RAPYITALRDLAAAPQPAVAST"
     gene            complement(3177..3893)
                     /locus_tag="VII37_00120"
     CDS             complement(3177..3893)
                     /locus_tag="VII37_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068148.1"
                     /translation="MTNYRLLALIIVGWLSWMMKPTAAASLTFATSSNAASLAASAVA
                     APAESAKLTFRRVFKSSSPEFIEIVVPEDARPATYDIRQLDEDPSASPFQISGGLRAH
                     MFELATQLNHFQGQDLDVHRKIANLGEKTFRWQKGEDVHEVKFNYTLNSAAAQLLQIF
                     EGLARQQEHVMLLTRRMRYDRLGINDALLQFETDLNHKLLPEPQCALPMLDEIGNDSR
                     FVDIARQRARTLAERIRHPS"
     gene            complement(3890..4633)
                     /gene="ruvC"
                     /locus_tag="VII37_00125"
     CDS             complement(3890..4633)
                     /gene="ruvC"
                     /locus_tag="VII37_00125"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY5068149.1"
                     /translation="MRKQIFWPGVVEGRRLVAVSGNNGRNQKDGFLRVLGVDPAAAGP
                     TGYGVVESDGRKSRMIHCGALSITAKRRKESCGAALQDVHELLSRLIEEYEPDAVAVE
                     AVFAALNVRTALRLAEVRGVVLLAAAQHNIVVHSYSPREVKSSVAGYGHADKRQVQEM
                     VRVLLTMQEIPEPSHAADALAVALCHLQAEQTRRRFGVPAVSKQARRSHGPALTHSPD
                     AATTVTGTVTGATRTYATLGAFPRIVPTR"
     gene            complement(4739..5494)
                     /locus_tag="VII37_00130"
     CDS             complement(4739..5494)
                     /locus_tag="VII37_00130"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5068150.1"
                     /translation="MSGHSKWATIKHKKAATDAKRGRVFTRVIREISIAARIGGGDPN
                     TNPRLRTAILAAKNENMPGDNIERAILRGTGQLEGEQYEEAVFEGYGPGGVGMLIQVV
                     TTNRNRIVGEFRHLMSKNGGNMAEAGAVGWMFSRKGEITVAKEAASEDKLLDIVLEAG
                     AEDMKDDGSIWYIVTPPEAMDKVKEALSKAGIAPASAEVGMVPQNYIKLTGAQATQMV
                     RLVEALEEHDDVQHVYANFDIDESEIQAAVGAN"
     gene            complement(5696..6376)
                     /locus_tag="VII37_00135"
     CDS             complement(5696..6376)
                     /locus_tag="VII37_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5068151.1"
                     /translation="MTLKEQRLQAAESSGGISSEVIYDRILKVIGDYHLRGRALDYGA
                     GIGNFTLKLSASGRFEQIDGADILPRPENLPSAILWHRLDLNDADAIPEASFDVVIAA
                     EVIEHLENPRQVAREWFRILKPGGTLILSTPNNESIRSLMALLVRGHYVAFLDSCYPA
                     HITALLRKDLMRILSEAGFSDIRFHFTDHGGLPKLPAVSWQQVSLGLLKGCRFSDNVI
                     ATARAPAG"
     gene            complement(6471..>6708)
                     /locus_tag="VII37_00140"
     CDS             complement(6471..>6708)
                     /locus_tag="VII37_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068152.1"
                     /translation="EEGSEIAAPITEAGVVLVPEELVPEGFGPALVRVLSDQNYRASL
                     AERSRDAQSRYFSWSVIARQYAQALRSLGTPSRK"
BASE COUNT         1441 a         2046 c         1683 g         1538 t
ORIGIN      
        1 cgccgtgcgc acctatccgc tttcttcgcg caaaagcaaa gtaaacgttg cagactttgc
       61 aaagccaccc gccaggaatg tctccatctc aaaattcatg gactcgctcc ccaattttct
      121 ggctgccgag gatttgcgcc aattgctcgc cgctgtccaa ctcgcgcgct ctggcaaaaa
      181 aacaattttg tggggcatcg gcggccacgt catcaaggtc ggcctcggtc ccatcctgat
      241 cgatctaatc gaacgcggat ttgtgtccgg catcgcgatg aacggtgccg cgctgatcca
      301 cgatttcgaa atcgctatag caggaaacac ttccgaggac gttgacgccg gcatccatgc
      361 aggtcaattt ggcatggctc gcgaaactgg agagcagctc aacgaaatcg ccaaaaacgc
      421 cgcgcgttcc ggcaccggtt atggcgaagc tgccggccaa ttcttgacca gcaccgccgc
      481 aaaagcccgc cacgcctctt ccagcgttgt ggccgcggcc tatcgccggc gcattccggt
      541 caccgttcat ctcgcgatcg gcgctgacat tcctcacatg catccagccg ccgatggtgc
      601 cgccctcggc gccgtgactt accaggactt ccggctcttc tgcgcgctgg tgaaatcaat
      661 gcatcctggc ggcgtatata tgaattgggg ctcggccgtg cttctgccgg agattttctt
      721 gaaagcggtc tcggtggtgc gaaatttggg cgtgccgttg cgccccatca ccaccgccaa
      781 ttttgatttc atccagcatt atcgtccgct gcaaaacgta gtgaagcgcc caacgttatc
      841 agtgagcaac cgccgcggga tagaatcgaa agggtacgcc atcaccggac atcacgaact
      901 acttatgcca ctggtagccg cggcgctggc ctcgggctgg ccaaaacgcg gcccaaagaa
      961 aaatagaaca tgaacgatca cgacgaaaac ttcgccaatg gaacgccgga ggacgccgtg
     1021 aacccgccga cggagactcc agcccagctc gctccgcccg taaccgaacc agttccggag
     1081 tctcaaccct cccaagtttt atctggcgat ccaatggccg ccgcgatccc tgccttccag
     1141 gctaagcgca ttctgccgga agatttgcga gtgtcctggt cttggccaca tctgattatt
     1201 tttatttttt ttgggtttgc cagcctgatg atcgttcaac tcggctttgt cttttatgtt
     1261 gccgcaaatc gtcacttaag tgccgtgcaa gtccagcgtg tatttgaaaa tagcccacag
     1321 ctgctggtcg aaaccaatgt cttgtggtac gccctgctgt ttctttttct ttatgtaact
     1381 ctagcggttt tacgcgacgt acctttctgg cgctcgctag gctggagaaa gttgagcgcc
     1441 aatccggcgc gcggcacagg cagcggctgg atgtatttct tctccggctg cggtttggcc
     1501 attttcgtcg ctctggccag ctacaacatg aaagataccg atcacctgcc gatccaggaa
     1561 cttttcaaga atcgttccgg cgcgctgctg cttatgtcca tggcggtttt tgtcgcgccc
     1621 ttggtggagg aaaccgtctt tcgtggctac ctctacccgc tcttcgccaa atcccttggc
     1681 gtgattccgg gaattttaat caccggcgta ttattcggtt tgatgcacgg ttcccagttg
     1741 ggctggactt tccggttggt attgctgctc acggtagtcg gcgtgatttt taccttcgcg
     1801 cgcgcccgta ctggcacagt tatggccagc ttcttgctgc atttaggcta taactcgatg
     1861 atcgctctga cgtcgattat cgccacgagg ggctttcagc atatgcccgt gtcgccttga
     1921 cttctcgtca acaaaacgat gtgcggcaat tcgttttgtt taacagcatt cgctacaatt
     1981 tattaaggcg cagctaaacg cgttcggaga ccttgggatt tatgtacgta gcacaaccga
     2041 ttgaatggac cgagcaaggc gtggtgatgc tggaccagcg ccgtctgccc gcggaagaaa
     2101 tcagctacac ctacactgat tatcgcgagg tggccaaggc cattcgcgaa atgatcatcc
     2161 gcggagctcc agccatcggc gtggccgccg ctatgggtgt ggcactagga gttttgcact
     2221 ccacggcaac atcggtcgat acattgaaat cggaatttgc tgaaatctgc gacacgctcg
     2281 cgaaaacgcg ccccacagcc gtcgatcttt tctgggcact cgaacgcatg aagcgtagat
     2341 ttgccgaatt gagcgccacc tccaagccac tcgtggaaat aaaacgggcc atgattgaag
     2401 aagcgaaatt agtgcaccta gaaaaaaagg ccaccgatca ggccatcggc gaatacggcg
     2461 ccgaattcat gcctgcggat ggccaagtaa tgacgcaatg caatgcaggc gcgctggcca
     2521 cgggcggaat cggaacggcc ttgggcgtga tccgtgtcgc ctatcagcaa ggcaagaagc
     2581 tgcacgtgct cgtcccagaa acccgtccgt atcttcaagg ctcgcgtcta accgcctggg
     2641 aactccacaa gggcggcatt ccactcacat taattaccga caacatggtt ggacatttct
     2701 tgaaaaccgg taaagttcgc gcgattgtga ccggcgctga ccgcatcgcc gccaatggcg
     2761 ataccgccaa taaaatcggc acatatcaaa ttgcggtact ggccaaggaa aacaacgtcc
     2821 cgttttatat cgccgcgcct gtttctactt ttgatttgtc cattcccgat ggcgaccaca
     2881 tccccatcga agaacgtgcc gcatcagaag tcacgcactt acagggcctc aggattgctc
     2941 ccgacgttca cgccgcgcat cccgcctttg acgtaacccc ggcccgttat atagctgcga
     3001 ttttcaccga acgcggcgtg gctcgcgcgc cgtacatcac cgcactccgc gacttggctg
     3061 ccgcgcctca gcccgccgtt gcttccacat gagattaata gctgcaactt tattattttg
     3121 atttttatcg atgtgcaaca ggaaaagcag ccgttgaggt ggcttcgtgc ttaacgctag
     3181 cttggatgtc gaattcgttc cgccaaagtc cgcgctcgct gccgcgcaat atctacaaag
     3241 cgcgaatcat tcccaatctc atccagcatc ggcagcgcac attgcggttc cggcagcaat
     3301 ttatgattca aatctgtttc gaattgcagc agcgcatcat tgatacccag gcggtcgtat
     3361 ctcatgcggc gcgtaagcag catcacgtgc tcctgctgcc gcgccaaccc ttcgaaaatt
     3421 tgcagtaact gcgccgccgc ggaattcaac gtgtaattga attttacctc gtgaacgtcc
     3481 tcgccctttt gccagcgaaa agttttttca ccgagattcg cgatttttct gtggacgtcc
     3541 aaatcctgtc cctggaaatg gttcaattgc gtcgccaatt cgaacatgtg cgctctaagt
     3601 ccgccgctga tttgaaacgg tgatgcgctg ggatcttcat ctaactgccg aatgtcatat
     3661 gtggccgggc gagcatcttc aggcactaca atctcgataa attccggaga gctggattta
     3721 aagacgcgcc tgaatgtgag tttcgcgctt tcggcgggtg ccgcaactgc agaggcagcc
     3781 agagaagcag cgttgctgga agtcgcaaag gtcaatgaag ctgctgccgt cggcttcatc
     3841 atccagctaa gccagccaac aataatcagc gccagcaaac ggtaattcgt catcgtgtcg
     3901 gtacgatacg cggaaaagcg cctagcgttg cgtatgtcct ggttgcaccc gttacggtgc
     3961 ccgttacagt tgtcgcggcg tccgggctat gcgtcagcgc aggcccgtgt gaacggcggg
     4021 cctgtttgct gaccgccggc acaccaaacc tccgtcgcgt ttgttccgct tgcaaatggc
     4081 acagcgctac cgccagcgca tcagccgcgt gcgacggctc gggaatttct tgcatcgtta
     4141 acagcactcg caccatttcc tgcacttgcc gcttgtccgc gtggccatat cccgccaccg
     4201 aggatttcac ttctcgcgga gaataactat gcaccacaat gttatgctgc gccgccgcca
     4261 aaagtaccac cccgcgcacc tccgccagtc gtagcgcagt cctcacgttc aacgccgcga
     4321 acaccgcctc taccgccacc gcatcaggtt catattcttc gatgagccgc gacagcagtt
     4381 cgtgtacatc ctgcagcgcc gctccacacg attctttgcg ccgtttagcc gtaatggaaa
     4441 gcgccccgca atgaatcatc cgcgacttgc gcccatcgct ttcaaccaca ccataacccg
     4501 tggggcccgc agccgccggg tcgactccca gaacacgaag gaaaccatct ttttgattgc
     4561 gtccgttgtt gccgctcacg gccacaagtc tacggccttc cacaactccc ggccagaaaa
     4621 tttgtttccg cattgcgcaa taaggttgcg caatcagaag gcccaaaaaa aattggccag
     4681 cgatggtatt cgccggccaa tgagtttatt tccaataaca aaagattccg cagaccggtt
     4741 aatttgctcc taccgctgct tgaatttccg actcatcgat atcgaagttt gcatacacat
     4801 gctgcacgtc atcgtgctct tcgagcgctt ccaccaatcg gaccatctgc gtagcctgcg
     4861 cgccagtcaa tttaatgtaa ttctgcggca ccatcccgac ctctgccgaa gccggagcaa
     4921 ttccggcctt gctcagcgct tccttaactt tgtccatcgc ttccggcggc gtcacgatgt
     4981 accaaatcga tccatcgtcc ttcatatctt ccgccccagc ttccaaaact atatccagca
     5041 acttatcttc gctcgccgct tccttcgcca cggtaatctc acccttgcgg ctgaacatcc
     5101 atcccaccgc tccggcttcc gccatgttgc cgccattttt gctcattaaa tgccggaact
     5161 cgcccacaat gcgattgcga ttcgtggtca ccacctgaat cagcatcccg actccgcccg
     5221 gtccgtatcc ttcaaacacc gcttcttcgt actgctcgcc ttccagttga ccggtaccgc
     5281 gcaaaatcgc gcgctcaata ttgtcccccg gcatattctc gtttttggcg gcgagaatgg
     5341 cggtgcgcag ccgcggattg gtgttagggt cgccgcctcc gatgcgcgca gcaatggaga
     5401 tttcgcgaat cactcgagta aaaacacggc cacgcttggc gtcagtagcg gccttcttgt
     5461 gcttgatggt tgcccatttt gaatgcccgg acatgacaca tcctctttat tcgttaatat
     5521 ttccgcagca atctgcggcc gtcaatgctg ggttagtgac agaccacaga catagtaaac
     5581 atgccaaatg gcccgctcga tgcacggcgg agactacggt gaaccgctac ccctgcgaaa
     5641 cctaggcagt ttagcacagg cgtataaccg tgggaacaca cacgtaccct gcttactacc
     5701 cggcaggggc cctggcggtc gcgataacgt tgtcgctgaa ccggcaaccc ttaagcagtc
     5761 ccaaagaaac ttgttgccag ctcaccgctg gcaattttgg caagcctccg tggtcggtga
     5821 agtggaagcg tatgtcggaa aacccggctt cgctcagaat gcgcatcaga tccttgcgaa
     5881 gcagagcggt gatatgcgct gggtaacaac tgtccaaaaa ggccacgtag tggccccgca
     5941 ccaacagcgc catcaacgac cggatacttt cgttatttgg cgtggataaa atgagcgtgc
     6001 cgcccggctt caaaatccga aaccattctc gcgcaacctg gcgtgggttc tccagatgct
     6061 cgatcacctc ggcagcgatc accacatcga agctggcctc tggaatcgca tccgcatcgt
     6121 tcaaatccaa ccggtgccag aggatggcgc tcggcaaatt ctcaggccgt ggcaaaatgt
     6181 ccgcgccatc aatttgctcg aaacggcccg acgccgacag cttaagcgtg aaattgccga
     6241 ttcctgcccc atagtcaagc gcgcgcccgc gcagatggta gtcgccaata accttcagga
     6301 tgcggtcgta gatgacctcg gaactaatcc cgccgcttga ttcggcggcc tgcaagcgtt
     6361 gctctttcag ggtcaccaag ggcgtccgta aatgttcatc agagttgtcg ctttgtgcgc
     6421 ccagtatact agcggtcgcc tcttactgct ggcagtacct tctgcagatc ttactttcga
     6481 gaaggagtgc cgagcgaacg caatgcttgg gcatattgcc gggcgatcac gctccaggaa
     6541 aaataacggc tttgcgcgtc gcgactgcgc tccgccaaag acgcccggta attctgatcg
     6601 ctcaagacgc gcaccagggc cggcccgaac ccctcaggca ccaattcttc cggcactaaa
     6661 accactcccg cttctgttat cggcgccgcg atctccgacc cttcttcg
//
LOCUS       DATCBB010000011         1476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_389541, whole genome shotgun sequence.
ACCESSION   DATCBB010000011 DATCBB010000000
VERSION     DATCBB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389541"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..522
                     /locus_tag="VII37_00145"
     CDS             208..522
                     /locus_tag="VII37_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY5068153.1"
                     /translation="MTPNAVTKVKEIMAQQNPVPSGLRVGVVGGGCSGFSYSMSFENT
                     PGMMDKVFDMGSLKVYVDATSVMYLNGCVVDYVETLEGAGFKFENPNVKSTCGCGSSF
                     SV"
     gene            complement(577..>1476)
                     /locus_tag="VII37_00150"
     CDS             complement(577..>1476)
                     /locus_tag="VII37_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068154.1"
                     /translation="AEGGYILLVLLLFVTLLAIAAAAVAPSIAFQVRRDREEEMIHRG
                     VQYSRAVRHYYKKFNRYPSKIEDLEDTNNYRSLRKRYKDPITGADFKVLHLSEVQMAL
                     GGGTIGGLTPPGSAPGAGANSQFGQPASGFGSGNFGGAATSPGAQSSSTDGTDAAGTG
                     AAGISAQSAFGSTGAGGKQPGGSSSNTGSDKLAGQTFGGGPIAGVVSTSKAQSIREFN
                     KKDHYNEWLFIYDPNSDRGGLLSTPSQPPLQGAGGLPGMPGAPSTSGSAFGNQGLGNQ
                     GLGNQGTPGMQPAPQSPQQPQQQ"
BASE COUNT          298 a          437 c          401 g          340 t
ORIGIN      
        1 cgcgattgaa atcccgagcc acgcgccttt tccgctcatt agcatcgttg aacttacctg
       61 cgcaatgact caaccccacc gacgcaaaaa actcgggcta ttcgtatcca gtttgctata
      121 attaagggtt cccaaggaga gagccctatg gctacaacga ctgtcccaga agttacccct
      181 gaggtgaaga aaacgccgcc gatttaactg actccgaacg ctgtaacaaa ggtgaaagaa
      241 atcatggcgc agcagaatcc cgtgcccagc ggtttgcggg ttggcgtcgt tggcggaggc
      301 tgctccggtt tttcctattc gatgtcgttc gagaacaccc ccggcatgat ggataaggtg
      361 ttcgatatgg ggagtctgaa ggtttacgtc gatgctacgt cggtgatgta tctaaacggc
      421 tgcgtagtcg attacgtcga aaccctcgaa ggcgcgggat tcaaatttga gaatccgaat
      481 gtgaagagca cctgtggctg tggatcttcg tttagcgttt aattcaagtt tgagcgaaca
      541 ggaacccgcc ttccggcggg ttttttattg ccgctattac tgttgctgcg gctgctgcgg
      601 gctctgaggg gccggctgca tgcccggagt tccctgattt cccaatccct ggttccccaa
      661 gccttggttc ccgaatgcag agccggatgt gcttggtgcg cctggcatac cgggcagccc
      721 gccagcacct tgcaatggcg gctggctggg ggtgctcagc aagcctcccc gatccgaatt
      781 cggatcgtag atgaatagcc actcgttgta atgatccttc ttattgaatt cgcggatgct
      841 ctgcgccttg ctggtgctca ccacaccggc aatcggtccg ccgccaaaag tctgccccgc
      901 cagcttatcg ctgcccgtat tggaactcga gccgccgggc tgtttcccgc ccgcacccgt
      961 gctcccaaat gcggattgcg ctgaaatgcc cgccgcacct gtaccggccg catccgtgcc
     1021 gtccgtggaa gaggactgcg cgcctggtga cgtcgctgcg ccgccaaaat tcccactacc
     1081 gaatccactt gccggttgcc cgaattgact gtttgccccg gctccagggg ccgaccccgg
     1141 cggcgtaagc cctccaatgg taccgccccc cagcgccatc tgcacctcac tgaggtggag
     1201 caccttaaaa tccgcgccgg taattggatc tttgtaacgt tttcgcaggg accggtagtt
     1261 atttgtatcc tccagatcct cgatcttgga tggatagcga ttaaatttct tgtagtaatg
     1321 ccgcaccgca cgcgaatatt gcacgccccg atgaatcatt tcttcttccc ggtctcgccg
     1381 cacctgaaat gcaatgcttg gcgcaaccgc cgcggctgct attgccagga gagtgacgaa
     1441 gaggagcagt acgagaagaa tgtaaccgcc ctcggc
//
LOCUS       DATCBB010000012         1527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_324732, whole genome shotgun sequence.
ACCESSION   DATCBB010000012 DATCBB010000000
VERSION     DATCBB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324732"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VII37_00155"
     CDS             complement(<1..652)
                     /locus_tag="VII37_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY5068155.1"
                     /translation="MAGVGFPFLMGASLYAQAPAEATTERITVTGSLIPTAEEVTAAP
                     LDTISTSDIAVSGGTADVLTILQKRNPDFVGGGNLGGSNANIASGSTQGGSIISLRGL
                     PTLVLLEGRRIADSAAIAAGGFQFTDVSLFPTSLISRIEVLKDGASALYGSEAVGGVV
                     NIFLKSDFNGVEVGARYGFTISSAVAERKAYAIAGVGNDTTHVTAGFQYYEIDPLFN"
     gene            1313..>1527
                     /locus_tag="VII37_00160"
     CDS             1313..>1527
                     /locus_tag="VII37_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068156.1"
                     /translation="MFTGLIEEVGSVRWIRATERGTQLQLAAPLITPGLRAGDSVSVN
                     GCCLTATAIREGNLTFDLLEETLARTNL"
BASE COUNT          321 a          436 c          432 g          338 t
ORIGIN      
        1 gattaaagag cggatcgatt tcataatact ggaagccggc cgtcacgtgc gtggtgtcgt
       61 tcccgacgcc cgcaatggca taggctttgc gctccgccac cgcactcgag atggtgaagc
      121 cgtagcgcgc accaacctca acgccgttga agtcgctctt caggaagatg ttgacgacgc
      181 cgccgaccgc ctccgaacca tagagcgcgg aagcaccgtc tttcaggact tcaattctac
      241 tgatgagcga agtcgggaaa agactgacgt cggtgaattg gaatccaccc gcagcgatgg
      301 ccgccgagtc cgcaatgcgc cggccctcaa gcaagaccag ggtcggcaag ccacgcagcg
      361 agatgatgga tccgccctgc gtcgagccac tcgcaatatt ggcgttcgag ccgccaaggt
      421 tgccgccacc gacgaagtcg gggttgcgct tttgcaggat ggtcaaaaca tcggcggtac
      481 cgccagagac ggcgatgtcc gaggtgctga tcgtatcgag cggcgctgcg gtcacttcct
      541 cggctgtcgg aatcagcgaa ccggtgacgg tgatacgctc ggtggtcgct tcggcgggcg
      601 cttgtgcata caggctcgcg cccatcagaa aaggaaatcc gacgccggcc acgagggccg
      661 cacgaagcac tcctatcgtt tttgttacgt tctttatcat tggtttctcc atttggtttg
      721 gttaggtttg gtggtgagga ttaaagaaga atttcacccc tgacaagcgt ttttttcatt
      781 ttttttgaaa tatttttgaa cgggttccgc ccaccgcgtg tcgcattctg ctgcccgggt
      841 cgccggaaag tcgctccgcc tgtctcatcc taacgaacaa tttcagcgcc atcgcggtga
      901 attttcttgg ggggaagccg gccgcgggtc gccgcatcca gcgaatgagg accagttctt
      961 cgtcgtgcgc tggagaacaa cgatcgcgcc taacaaggtc ccgccgcgat accaaggatg
     1021 cgccgtcctt ttttccagtc tgggactaat cttgtgggcg tgttgctcaa atcggcttta
     1081 gccacaaatg ttgctaagtg agggggttca aatcaccctg ccgcagaatc aaaatgcgct
     1141 acaggtcatt ctgtgacttc gtcttttacc ctcattacag aaagggcgtt tgagcaacac
     1201 gcacttgttc ttttggtgcg aaaatgagtt cggctgatcc cgcacggact ggcacgtgcg
     1261 caaaaatctc tccactcaat ctgcgaaaat ccgcgtaatc tgcggacgta atatgtttac
     1321 cggattaatc gaagaagtgg gcagcgtgcg ctggattcgc gccaccgaac gcggcacgca
     1381 attgcaactc gccgcaccgt tgatcacccc gggcctgcgt gcgggcgaca gcgtctccgt
     1441 gaatggttgc tgcctcacgg cgaccgcgat tcgcgagggt aacctgacct tcgacctgct
     1501 cgaagaaacg ctcgcacgaa ccaatct
//
LOCUS       DATCBB010000013         1163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_162586, whole genome shotgun sequence.
ACCESSION   DATCBB010000013 DATCBB010000000
VERSION     DATCBB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162586"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VII37_00165"
     CDS             <1..280
                     /locus_tag="VII37_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068157.1"
                     /translation="DVCVEVRIATQRPPAETLNVCSGDSRDKPVANERYLGLENPNSL
                     ALSVHLIVVSVCTIRHIHRVLDVRPLAILHNAVVPKFSEIGAARIRGG"
     gene            complement(609..683)
                     /locus_tag="VII37_00170"
     tRNA            complement(609..683)
                     /locus_tag="VII37_00170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Val,seq:cac)
     gene            738..>1163
                     /locus_tag="VII37_00175"
     CDS             738..>1163
                     /locus_tag="VII37_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY5068158.1"
                     /translation="MGSGLWLCIARLPWDHCRMTVITRFAPSPTGLLHVGGVRTALFS
                     WLYARHRGGKFILRIEDTDRERSTDAAVRVILDGMKWLGLDADAGPYFQTQRYDRYRE
                     VIARMLRDGSAYHCYCTKLELEELRAQQLASKEKPRYSGL"
BASE COUNT          239 a          309 c          349 g          266 t
ORIGIN      
        1 cgatgtttgc gtggaggtgc gaatcgccac gcaacggcct cctgccgaaa cattgaacgt
       61 ctgtagcggt gattcacgcg acaagcctgt cgcgaacgag cgctatttag gtctggaaaa
      121 tcctaattcc ctggcactca gcgtccactt aattgttgta tctgtatgta cgataaggca
      181 catacaccgg gttttggatg tgcgcccgtt ggcaattttg cataacgccg ttgtgccgaa
      241 attcagcgag attggtgcag cgcgcatccg cggtggctag cgcgtgtatt tgcccgtact
      301 ctcattgaca actgttgatg ctgaccaact gaatcgatag ttgacgcaaa gttggggttg
      361 tgcaatcgcg ccaaccctat atggacgttt aagtcgcatg tggatggcag cgcttaagaa
      421 cttctgcaag gccgaaaccg ctgccgcaat atcgctattc acttctggat cattaatagc
      481 tcaaggtgcc cctgcggaat tagccagtgg cgctcgggtc ggagagcttg gatgggactt
      541 gggacgtaga agttccgcct gcgcttgccg tacgacgtga gatgatcgtc gtaagatatt
      601 gatttttatg gtgggcggta cagggatcga acctgtggcc cctgccgtgt gaaagcagtg
      661 ctctaccgct gagctaaccg cccgccgtca gtcgcagcga agtgtacggg ccgcagcctg
      721 cgtgaatcaa tgactgcgtg ggaagtggtc tttggctgtg catcgcgcgc ttgccgtggg
      781 accattgtcg catgacggtt atcacgcgat ttgctccgag cccaacgggc ctactccatg
      841 tcggcggcgt acgcacggcg ctgttcagct ggctctatgc gcgccaccgc gggggtaagt
      901 tcatcttgcg aattgaagat accgatcgcg aaagatcgac cgatgcggcg gtgcgggtca
      961 ttcttgatgg catgaaatgg ctcggcttag acgctgatgc gggaccgtat tttcagacgc
     1021 agcgctacga ccgataccgt gaggtcatcg cgcgaatgct gcgcgatggc agcgcctacc
     1081 actgctactg cacgaaactg gagctcgagg aactgcgcgc ccagcagctc gccagcaaag
     1141 agaagccccg ctattcgggc ctg
//
LOCUS       DATCBB010000014         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_195045, whole genome shotgun sequence.
ACCESSION   DATCBB010000014 DATCBB010000000
VERSION     DATCBB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195045"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VII37_00180"
     CDS             <1..992
                     /locus_tag="VII37_00180"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY5068159.1"
                     /translation="KDRLHLPGPDFIDRIWAISDRNIRVLTNLQRMFGRGRLANTGYL
                     SILPVDQGIEHSAGASFAKNPDYFDAENIVKLAMEGQCNAVASTFGVLGAVARKYAHK
                     IPFIVKMNHNELLTYPNKADQILFGTVKEAYDMGAAAVGATIYFGSEQSGRQIVEISQ
                     AFATAHELGMATVLWCYLRNNAFKKDKDYHLSADLTGQANHLGVTIQSDIIKQKLPEN
                     NGGYAALNMGDSSYGKFDKRIYTDLSSDHPIDLCRYQVANCFMGRSGLINSGGASGAN
                     DFEEAVTTAVINKRAGGTGLISGRKAFQRPMKEGVQLLNAIQDVYLSSEVTVP"
     gene            1146..1919
                     /locus_tag="VII37_00185"
     CDS             1146..1919
                     /locus_tag="VII37_00185"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5068160.1"
                     /translation="MSNRLQGKVVAITGGNQGIGLGIAQRFVQEGAAVSICYLSDKPG
                     TEKVSESLRANGAKAVAIQADVSKLADGQRFVAETAQQLGEIDVLINNAGVEKRAEFW
                     EVTEADYDFVLNVNLKGLFFITQAFVKHQMQAKAGGKIINISSVHEELPFPHFATYCA
                     SKGGVKMLTRDLSIELAPLGITINSIAPGAIETPINSALLHDPAKLKALLENIPLKRL
                     GQPGDVASVAVFLASDESSYITGTTVFVDGGLLWNYQEQ"
     gene            1936..>2164
                     /locus_tag="VII37_00190"
     CDS             1936..>2164
                     /locus_tag="VII37_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY5068161.1"
                     /translation="MTTANPQKPVRLVIADVDGTLVTQEKILTQRAIQSVMALRGAGI
                     KFAVTSGRPPRGMAMLIDPLRIDEPLAAFNGG"
BASE COUNT          502 a          555 c          611 g          496 t
ORIGIN      
        1 cgaaagatcg tttgcatttg ccggggcccg attttatcga tcgcatctgg gctatttcgg
       61 atcgcaatat tcgcgtactt actaatttgc agcgcatgtt tggccgcggg cggttggcga
      121 acaccggata tctgtccatt ctgccggtcg accagggcat cgaacattcc gccggggcga
      181 gcttcgcgaa aaatccagat tatttcgacg cggaaaacat cgtcaagctg gccatggagg
      241 gccaatgcaa cgcggtggct tccactttcg gcgttttggg cgctgtggca cgcaagtatg
      301 cgcacaaaat tcccttcatc gtaaaaatga atcataacga gttgctcacc tacccgaata
      361 aagccgatca gattttgttt ggcacggtga aggaagctta cgacatgggc gccgcagcag
      421 taggcgccac gatttatttt gggtcagaac agagtggacg acagattgtg gagatcagcc
      481 aggcgttcgc aaccgcgcat gagctcggca tggctacggt tttgtggtgc tacctgcgca
      541 acaacgcttt taagaaggat aaggattatc acctctccgc cgatctcacg ggtcaggcca
      601 accacctggg cgtgactatt cagtcggaca ttatcaagca aaagctgccc gaaaataatg
      661 ggggctacgc agccctgaac atgggcgatt ccagttacgg gaaattcgat aagcgcatct
      721 atacagatct ttccagcgat catcctatcg atctctgccg ctaccaagtg gctaattgct
      781 ttatgggacg ctctggactg atcaacagcg gcggcgcatc gggcgcaaat gatttcgaag
      841 aagcggtgac caccgcagtg atcaataagc gtgcgggcgg caccgggctc atctctgggc
      901 gaaaagcgtt ccaacgtccg atgaaagaag gcgtgcagtt gttgaacgcc attcaggacg
      961 tttatctgag ctcggaagtt accgtcccgt aggcaatcgg cgcttggcgt attgtttgtt
     1021 gaaaaataag tgatcgttcg gtcgctgctg atcatcaaag gttccgggac cgccggagcg
     1081 attattccgt agcttgcttt cgataattag ccctttccac attcgcgaaa atgaccggag
     1141 ctgaaatgtc caatcgtttg caaggcaagg ttgtagccat caccggaggc aatcaaggta
     1201 ttggacttgg aatcgcgcaa cggtttgtgc aggagggcgc tgctgtctcg atttgctatc
     1261 tctcggacaa gccggggacg gaaaaagttt ctgagagtct tcgcgcaaat ggggcgaagg
     1321 ccgttgcgat ccaggcggat gtctccaaac ttgcagacgg gcagcgcttt gtggcggaaa
     1381 cggcgcagca gcttggcgaa atcgatgtgc ttatcaacaa tgcgggcgtg gaaaagcggg
     1441 ctgaattctg ggaagttacc gaagccgatt acgattttgt tttgaacgta aatctaaaag
     1501 ggctgttctt catcacgcaa gcgtttgtca aacatcagat gcaggccaag gccggcggca
     1561 aaatcattaa catcagttcc gttcacgaag agctgccctt tcctcatttc gccacatatt
     1621 gcgccagcaa aggcggcgtg aaaatgttga cgcgcgacct ttcgattgag ctcgcgccgt
     1681 tgggcatcac tataaattcc atcgcgcccg gagcgattga gacgcctatc aactctgcgc
     1741 tgttgcacga tccggccaag ctgaaggctc tgcttgaaaa cattcccctc aagcgccttg
     1801 ggcagccggg cgatgtagcc tccgtcgcgg tttttctggc ttccgacgaa tcgagctata
     1861 tcaccggcac tacagttttt gttgatggcg gcttgctctg gaattatcag gagcagtgac
     1921 gctcgcgagg accgcatgac cactgcaaat cctcaaaaac cagtgcggct ggttatcgct
     1981 gatgttgacg gcacgctggt gacgcaggaa aaaatactga cgcagcgcgc cattcaatcg
     2041 gttatggcgt tgcggggcgc gggcatcaag ttcgcagtca ccagcggccg tccaccgcgc
     2101 ggcatggcca tgctgatcga tccgctgaga atcgatgagc cgcttgccgc attcaatggc
     2161 ggtg
//
LOCUS       DATCBB010000015         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_259924, whole genome shotgun sequence.
ACCESSION   DATCBB010000015 DATCBB010000000
VERSION     DATCBB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259924"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VII37_00195"
     CDS             <1..1173
                     /locus_tag="VII37_00195"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEY5068162.1"
                     /translation="YVATDAQGQWLALLVFNAPAKHLKFRDEWIGWNAAQRERRLALV
                     VNLSRYCLLPDKTFPNLGTRVLRLTLDRLSADWQSHYGHPVLIAETFVDPQQFCGTVF
                     TANGWIELGQTDGWGRHQRDYYVKHDKPKRLFARQLCPNARRSLQAEHLKPDLAGVET
                     KVTPRPRRTVKEIASMAEEFKKIPDFRVHFESYPLWSLLTIVLLAVLCESSRGSKALA
                     KFARGFSQPQRRALGIRRNPKGRYPAPSQPTFCRCLQKVDGRKLEETILAIQEKMRGQ
                     PPKGDLIALDGKEPKHGGGQGVLTAVSLPGQFYLGSEIVEEKTNEIPVAPVLLERLDL
                     QGRFVSLDALNTQDQTARAIVLEGGGDFLLTAKGNQPTAEANIKKLVPAPKADFSP"
     gene            1253..1669
                     /locus_tag="VII37_00200"
     CDS             1253..1669
                     /locus_tag="VII37_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068163.1"
                     /translation="MSPEQAAFPLVAQAARLLRQTSGRKEEEVCLLTSAPADKLDAQE
                     WLHLNRNYWGIENGLHQRLDVSHDDDRCRIRKTPGLWVMGMFRRLSNSFCLEWCSCQT
                     HPEHKTTTDFQSAMAEDQRRTALRLVFSKNPKFRAG"
     gene            complement(1808..>2128)
                     /locus_tag="VII37_00205"
     CDS             complement(1808..>2128)
                     /locus_tag="VII37_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5068164.1"
                     /translation="AKEDYEYKRAGTRNLFVAVEPKGCRREVEVTARRTKSDFVQFIG
                     HLVETVYAAAKKIHLVLDNLNTHFPSSFVEVMGADRANSWRRRQAWRWRYECRGEILR
                     GSNS"
BASE COUNT          464 a          682 c          557 g          425 t
ORIGIN      
        1 tacgtggcca ccgacgccca gggccaatgg ctggccctgt tggttttcaa tgcccccgcc
       61 aaacacttga agttccgcga tgagtggatt ggctggaacg ccgcccaacg cgagcgccgg
      121 ctggccctgg tggtcaatct cagccgctat tgtctattac ccgacaaaac ctttcccaat
      181 ctaggcaccc gggttttgcg tctgacactg gatcgcttga gcgctgattg gcaatcccac
      241 tacggccacc ccgtcttgat cgccgagact tttgttgatc cccaacaatt ctgcggcacc
      301 gttttcaccg ccaacgggtg gattgaactg ggccaaaccg atggctgggg acgccatcag
      361 cgcgattact atgtcaagca tgacaaaccc aaaagactct ttgcgcgcca actctgcccc
      421 aacgcccggc gcagcctcca agccgagcat ctcaagccgg acctggcggg ggtggaaacc
      481 aaagtcactc cgcggcccag acgcactgtc aaagaaatcg cttcgatggc cgaggaattc
      541 aaaaagatcc ccgactttcg agttcacttc gaatcctatc cgctctggtc gctgctgacc
      601 attgtgctcc tggcggtgct ctgcgaatcc tcgcgcggtt ccaaagcgtt ggcgaagttt
      661 gcccgcggat tcagtcaacc ccaacgacgg gccttgggca ttcgccgcaa tcccaaaggc
      721 cgctatccgg ctcccagcca acccaccttc tgccggtgcc ttcagaaagt cgatggccgc
      781 aagctcgaag aaacgatttt ggcgattcaa gaaaaaatgc gcggccagcc gcccaaaggg
      841 gacctcattg cgctggacgg caaagaaccc aaacatggcg gcggccaggg cgtgctcacg
      901 gcggtgagcc tgcccggcca gttctatctg ggcagcgaaa tcgtcgagga aaaaaccaac
      961 gaaatcccgg tggctcccgt attgctggaa aggctggatt tgcaagggcg ttttgtcagc
     1021 ttggacgccc tgaacactca ggaccagacc gccagggcta ttgtgctgga gggcgggggt
     1081 gattttctgc tgaccgccaa aggcaatcag cccaccgcgg aagccaacat caaaaaatta
     1141 gtgcccgctc ccaaagcgga tttttccccc tgagaatccc acgcccaccc aatgccgcac
     1201 ggccgaaaag aacaagggcc gcgccgaaag ccgctccatt gtgactcaag ccatgtcgcc
     1261 cgaacaagcg gcctttccct tggtcgccca agccgcgcgc ctgctgcgtc aaacttccgg
     1321 ccgcaaggag gaggaagtct gcttgttgac cagcgccccg gccgacaaac tcgacgccca
     1381 ggaatggctc catctaaatc gaaactactg gggcattgaa aatggcttgc accaacgatt
     1441 ggacgtttcg cacgacgacg atcgttgccg catccgcaag actccgggct tgtgggtcat
     1501 gggcatgttt cgccgtttga gcaacagttt ttgcctggag tggtgctcct gccagaccca
     1561 ccccgagcac aagaccacga ccgatttcca atccgccatg gccgaggatc agcgccggac
     1621 ggctctgcgc ctggtttttt ccaagaaccc gaaatttaga gccggatagc ggcggcgcca
     1681 aaccgcgcac aatccgccaa gagttcagtc cgcgatgaac ttcaagccag cgttttgtct
     1741 tgaagccctt ccaactccta attgccaccc ccacatcgcc attgtttgag ggcgcgttga
     1801 gtccacctca agaatttgaa cccctcagaa tttcaccgcg gcactcgtac cgccatctcc
     1861 aggcctgccg cctcctccat gaattggccc tgtccgctcc catcacttcc acaaaggagc
     1921 ttggaaaatg ggtgttcagg ttgtccaata ccagatgaat tttcttggct gcggcataga
     1981 ccgtttcaac cagatggcca ataaattgca caaaatcgga tttggtccgt cgtgcggtca
     2041 cttccacctc ccggcggcac cccttgggtt cgacggcgac gaaaagattg cgcgttccgg
     2101 cccgcttgta ttcgtagtct tccttggc
//
LOCUS       DATCBB010000016         1443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_65280, whole genome shotgun sequence.
ACCESSION   DATCBB010000016 DATCBB010000000
VERSION     DATCBB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65280"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VII37_00210"
     CDS             complement(<1..1192)
                     /locus_tag="VII37_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5068165.1"
                     /translation="MRLLSPLLYTAYLWAFLLAIPTAVASDAPPQPADIIITNARIYT
                     VNPQQQWAEAIAVRGDRIVAVGSSEEIARYRGASTLAIDAKHHLVLPGFVDCHIHFLD
                     GSLSLLQVNLDQARAVAEIQQQVKAYASAHPELPWVLGRGWQYTAFRDGLPTKEILDQ
                     IIPDRPVYLESFDGHSWWANSKALKLAGITRETPDPAGGKFVRDAGGEPTGAVKEDAA
                     DAVVRRAIPPPTHQGVLDALRGGMHEANKVGLVRGISPGGVSVQGGDFYALDAYDELR
                     RQKQLTMRFYIAARMEPPTVGKEQIDKIEDARRRYHDEWLAAGAAKFFLDGVIESHTA
                     AMLAPYSDDPSQSGKLLWDPDIYKQAVADLDRHGVQVYTHAIGDRAVRLALDSYAEAQ
                     SKNGK"
     gene            complement(1210..>1443)
                     /locus_tag="VII37_00215"
     CDS             complement(1210..>1443)
                     /locus_tag="VII37_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5068166.1"
                     /translation="PHLFRIAPDAGSGESYSHEGEEFLFVLRGELQIALDGEEYHLKR
                     GDSFYFESATPHHWKNPGRNETWLLWVNTPPTF"
BASE COUNT          294 a          406 c          415 g          328 t
ORIGIN      
        1 gtttgccatt ttttgactgc gcttcggcgt atgaatccag agccagacgc accgctcggt
       61 cgccgatggc gtgggtataa acctgaacgc catggcggtc gaggtccgct accgcttgct
      121 tgtatatgtc aggatcccaa agaagtttgc cgctttggct cggatcatcc gaatagggag
      181 ccagcatggc tgcggtgtga gattcgatca cgccatcgag aaagaacttt gccgcgcccg
      241 ctgccagcca ttcgtcgtgg tagcgtcgcc gtgcatcctc aatcttgtcg atctgttctt
      301 tgccgacggt gggtggttcc attcgcgctg cgatgtaaaa gcgcatggtc aactgcttct
      361 gacgtcgcaa ctcgtcatag gcgtcgagag cgtaaaaatc gccaccctga acgctgaccc
      421 cgcccggact gatgccacgc accaacccaa ctttgttcgc ttcgtgcatt ccgccacgca
      481 atgcgtcgag cactccctga tgcgtcggcg gcggaatggc gcgccgaacg accgcgtcgg
      541 cggcatcttc tttaaccgcg ccggtaggtt cgccaccggc gtcgcggaca aactttcctc
      601 ctgcagggtc tggtgtctct cgtgtaattc cggccagctt cagcgctttg ctattcgccc
      661 accaagagtg gccatcgaaa ctttccagat agacgggacg gtcgggaatt atctgatcga
      721 gaatttcctt ggtcggcagt ccatcgcgga atgccgtata ttgccatccc cgccccaaga
      781 cccacggcaa ttcgggatgt gcacttgcat atgcttttac ttgttgttga atctctgcga
      841 cggcacgtgc ctggtccaaa ttgacctgta acaggctgag cgatccgtca agaaaatgga
      901 tgtggcagtc gacgaagccg ggaagaacga gatgatgttt ggcatcgatc gccaacgttg
      961 aagcgccacg gtaccgcgca atttcctcac tgcttcctac agccacaatc ctgtcgccgc
     1021 gaaccgcgat cgcctccgcc cattgctgct gcggattgac ggtgtaaatg cgggcattgg
     1081 taatgatgat gtcggcaggt tgcgggggcg cgtctgacgc gacagcggta ggaatcgcaa
     1141 gcaggaatgc ccacaggtat gcggtatata ggagcggcga gagaaggcgc atagtggtga
     1201 ttcctgtact tagaacgttg gcggagtatt gacccacagc agccaggttt cattgcggcc
     1261 gggatttttc cagtgatgcg gcgtggcgct ctcaaaataa aagctgtcgc cgcgtttcag
     1321 atggtactcc tcgccatcga gagcgatttg caactctcct cgaagcacga ataagaattc
     1381 ctcaccttcg tgggaatatg actcgccgct gcctgcatcg ggtgcgatgc ggaaaagatg
     1441 ggg
//
LOCUS       DATCBB010000017         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_389867, whole genome shotgun sequence.
ACCESSION   DATCBB010000017 DATCBB010000000
VERSION     DATCBB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389867"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="VII37_00220"
     CDS             complement(<1..1595)
                     /locus_tag="VII37_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5068167.1"
                     /translation="MASLFSPQLKRLLAYVRPYSLRLVAGILLLAFVALAEGGVALMV
                     APAVDRVLNPAAVGSLLALVKVPWSGRTIYLNDFFPPRIHNVWTVFAFALLILYFSKA
                     VAEYLGSTEIQYVGQAAVTDLRNQVYARLIRQPMGFFQHNSTGRLNSTVINDVERTRV
                     ALSEYLADLFQKGFTFLVFVCVLLVVNWKMALGAALLLPLVILPVNKFGRKIRRSVET
                     SQSRLGDLSQILQETVSGNRVVKAFGMEPFEIRKFREASRRLLRENMRWVRAGVITSP
                     LMDLLGAIVIPLLLLYARGAIQHRVMTTGQFFTFIYAMFNAYMPLKRMGNVYQQFQAA
                     RGASTQVFAYLDLEPENIEQPGAQILPAFAREIAFEHVSFSYTPGSTMILQNVSLQAR
                     KGDVIALVGSSGAGKTTLVNLLPRFYEVTAGTIRIDGKDIREVTIRSLRDQIAMVAQE
                     NILFYDTVWNNICYGLTGVAEERVLAAARAALAHDFSQELPLGYNTLVGERGTRLSGG
                     QRQRIAIARAILKDSPILILDEAT"
     gene            complement(1713..2591)
                     /gene="rapZ"
                     /locus_tag="VII37_00225"
     CDS             complement(1713..2591)
                     /gene="rapZ"
                     /locus_tag="VII37_00225"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY5068168.1"
                     /translation="MKRPPTRHDARHLVVLTGLSGSGKSTVLKSFEDMGFYCVDNLPV
                     ELMPIFAELHAAGEGDFARAALMIDAREGLQLQKLPPLLKHLRKDHPITLVFIEAHDD
                     ALLRRYSETRRPHPLGKDYSVRESLRHERDLMAPIRKLADVVIDTTQFNVHELRHFVT
                     ERFKNPDKRPLMVSVVSFGYRYGVPTDADLVFDVRFLPNPHFVPRLREFTGKDPKVRR
                     YIRSFPQTAEFLRRVEGLLSYLIPHYAREGKSYLTIAFGCTGGRHRSVMMSEAVKKYL
                     EKRGTTAKVVHRDIEK"
     gene            complement(2605..>3247)
                     /locus_tag="VII37_00230"
     CDS             complement(2605..>3247)
                     /locus_tag="VII37_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068169.1"
                     /translation="KTEPRLVEPDVYFRKVDDQWVAFLNEDDMPQLRLSPTYRRLLVK
                     DASDRDVRNYVKERFTAAIQLMKNIEQRKHTILRVCQSIIARQGEFLDHGQDALKPMM
                     IKEVAEEVGVHPSTVSRAVASKYAHTPQGVLELRSFFSESVNGPEGGAMSLLTLKRVV
                     KKMIEEEDGSKPLTDEQIAKKLDDAGIHVTRRTVAKYREDMRIPSTHQRRVKS"
BASE COUNT          749 a          971 c          803 g          724 t
ORIGIN      
        1 gtcgcttcat cgaggattaa aatcggcgaa tccttcagaa tcgctcgtgc gatagcgatg
       61 cgctgccttt gtccgccgct cagtctggtt ccgcgttcgc ccaccagcgt gttgtaaccc
      121 agcggcaact cctggctgaa atcatgagcg agtgccgccc gcgcggccgc cagcacccgc
      181 tcttcagcaa ccccagtcag cccataacaa atgttgttcc acaccgtatc gtagaaaaga
      241 atattttcct gtgccaccat ggcgatctga tcccgcaggg aacggatcgt aacttcgcga
      301 atatccttgc catcgattcg tatcgtgccc gcggtcactt cataaaaacg cggcagcaga
      361 ttcacaagcg tggtttttcc ggctccgctc gagcctacca aggcaatcac gtcgcccttt
      421 cgtgcttgca acgatacatt ttgcaaaatc atcgttgagc ccggggtgta actgaaactg
      481 acatgctcaa atgcaatctc acgtgcgaac gctggaagga tctgggcgcc aggctgttcg
      541 atattttccg gttccaggtc aaggtaagcg aaaacctgcg tactcgctcc tcgtgcggcc
      601 tgaaattgtt gatacacatt tcccatgcgc ttcagcggca tataggcatt gaacatcgcg
      661 taaataaacg taaaaaattg tcccgtggtc atcacacgat gctgtatcgc cccgcgtgca
      721 tacagcagca acaaaggaat gacgatggca ccgaggagat ccatcagcgg cgacgtgatg
      781 actccagcgc gcacccatcg catattttcg cgcaacaacc tcctcgatgc ttcgcggaat
      841 ttgcgaattt caaatggctc cattccaaac gctttcacaa cccggtttcc gctcaccgtt
      901 tcctgcaata tttggcttaa gtctcccagc cgcgactggc tcgtttccac agatcgccga
      961 atcttccggc caaatttatt aaccggcagg atcaccagcg gcaaaagaag cgccgctccc
     1021 agcgccatct tccaattcac gaccaacaac acgcaaacaa aaaccagaaa cgtaaatcct
     1081 ttttgaaaca ggtccgctag atattcacta agcgcgacgc gggtgcgctc cacatcatta
     1141 atcaccgtgg agttcagacg cccggtggag ttgtgttgaa agaaacccat cggttgccgg
     1201 atcaatcgcg catacacctg gttgcgcaga tcagttaccg cggcctgccc cacgtactgg
     1261 atttccgtgc tccccaggta ttccgcaacg gccttcgaga aataaagaat caacagggcg
     1321 aacgcgaaaa cggtccacac gttatgaatt cttggcggaa aaaaatcgtt gagataaatc
     1381 gttcgtcccg accagggtac cttaaccagc gccaacagcg atcccaccgc cgcaggattc
     1441 aatacgcgat ccaccgcggg agccaccatt agcgccacgc cgccctcggc cagcgccaca
     1501 aacgcgagca gcaatatgcc ggccaccagg cgtaacgagt acggccgcac gtaagccaat
     1561 agccgcttca attgtggtga gaacaacgac gccacgcttc agactccccg aacgcttctc
     1621 ttggtacgct attcgccgct agagggtgtg tcaagttatc gcccggtctg gcagcgagct
     1681 tccaggatcc tcaagaaacc ggcgacccaa gctcattttt ctatgtcgcg gtgcacaact
     1741 tttgcggtag tcccgcgctt ttccaggtat ttttttaccg cttccgacat catcacggag
     1801 cggtgccttc ctccagtgca gccaaaagca atcgtcaaat aacttttgcc ttcgcgcgcg
     1861 taatgaggaa tcaaatacga cagtagccct tcgacgcgcc gcaaaaactc cgccgtctgc
     1921 ggaaacgaac gaatatacct gcgcaccttg gggtctttcc cggtaaattc ccgcagccgc
     1981 ggaacgaagt gtggattcgg caggaagcgc acatcaaaaa ccaggtccgc atccgtcggc
     2041 actccgtaac gatatccaaa actcaccacc gataccatca aaggccgctt gtcaggattt
     2101 ttgaaccgtt cggtaacaaa gtgccgcaac tcgtgcacat taaactgcgt ggtgtcgatt
     2161 accacgtccg cgagtttccg gatcggcgcc atcaagtcgc gttcgtgccg cagactctcg
     2221 cgcaccgaat aatctttgcc cagcgggtgc ggccgccgcg tttcgctata acggcgcagt
     2281 aacgcgtcat cgtgtgcttc gataaacacc agcgtaatcg ggtgatcctt gcgcaaatgt
     2341 ttgagcaagg gagggagctt ctgcagttgt agcccctcgc gcgcatcgat catcaaagcc
     2401 gcgcgcgcaa agtcgccttc tcccgcggca tgcagctccg cgaaaatcgg catcagctcg
     2461 acaggtagat tgtcgacgca ataaaagccc atatcttcga aggattttag gactgtgctt
     2521 tttccggagc cggaaagtcc agtgagaacg acgaggtgtc gcgcgtcatg tctcgtcggc
     2581 ggccgtttca ccggggcgcg cgccttaact cttcactcgg cgctggtgcg tgctaggaat
     2641 tctcatatct tcacgatatt tcgctacagt gcggcgcgta acgtggatgc ccgcgtcatc
     2701 cagcttcttc gcgatctgct catccgtcag cggcttcgaa ccatcttctt cttcgatcat
     2761 ctttttcact acacgcttca gggtcagcag tgacatcgcg ccgccctctg gcccgtttac
     2821 agattcgctg aaaaatgacc gcaactccag tacgccttgg ggagtgtgag catacttgct
     2881 ggctaccgcc cggctgacgg tggaaggatg cacgccaact tcttccgcca cttctttaat
     2941 catcatgggc ttcagggcat cctggccgtg atccagaaat tcgccctgtc gggcgataat
     3001 ggactggcac acgcgaagga tggtgtgttt tctctgctcg atgtttttca tcaactggat
     3061 cgcggcggta aaacgctctt tgacgtaatt gcgtacatcg cgatcagagg cgtcttttac
     3121 cagtaaacgg cgataggtcg ggctcaggcg gagctgaggc atgtcgtctt cgttcaggaa
     3181 agcgacccat tgatcgtcta cttttcgaaa atagacatca ggctcaacca gacgaggctc
     3241 ggtcttg
//
LOCUS       DATCBB010000018         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_65483, whole genome shotgun sequence.
ACCESSION   DATCBB010000018 DATCBB010000000
VERSION     DATCBB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65483"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VII37_00235"
     CDS             complement(<1..1337)
                     /locus_tag="VII37_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5068170.1"
                     /translation="MKSAANQSYRVLLADDQSDIRDALRLLLKREGYETHGAASPADA
                     LAAIESREFDAVLMDLNYARDTTSGQEGLDLLTRIQMLDSTLPVVVMTAWSSVEIAVE
                     AMRRGARDFVQKPWENNRLLSIVRTQIELRQALRQASRLEAENRLLRAESRPTFLAKS
                     PAMAPVLDLVARIGPSDANVLITGEHGTGKEVVARTLHALSNRASKPMVTVNAGGLSE
                     GIFESELFGHVKGAFTDARSDRVGRFELADGGTLFLDEIANVPLNLQAKLLRVLETGE
                     LERVGSSATRRVDVRVLAATNVDVNSEAAAGRFRQDLLFRLNTIEIFIPPLRERREDI
                     DLLAHHFVHGYSQRYRKNIGGFDSAATAALHEHLWPGNVRELDHAVERGVLLSAGTLV
                     RAADLGLRAQASTAAARLEEMPLDEVERHLIRRTLARHDGNVSQAAKALGLSR"
     gene            complement(1438..2130)
                     /locus_tag="VII37_00240"
     CDS             complement(1438..2130)
                     /locus_tag="VII37_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5068171.1"
                     /translation="MIQFRSLEKSYDAGGSKFFVLRRINLEIQQGEFVTIMGPSGAGK
                     STLLSIIGMLDSAWTGEYFFLGEAVHHLNAKKRNELHKANIGFVFQSYHLIDNLTVFE
                     NLEVPLSYKNVPHKERVSLVCDALDRFHIVGKKDLFPNQLSGGQQQLVGVARAVILHP
                     KLLLADEPTGNLHSAQGDEIMQLFKKLNDAGTTIIQVTHSERNAAYGNRIVQLKDGWV
                     VSENAIATVQSA"
BASE COUNT          537 a          710 c          634 g          500 t
ORIGIN      
        1 ctgcgactca gccccagcgc tttggccgcc tggctcacgt taccatcgtg gcgtgccagc
       61 gtgcgccgga taaggtggcg ctccacttca tcaagcggca tttcctccag ccgcgccgcc
      121 gccgtcgatg cttgagctcg caaacccaga tcggcggcgc gtaccagcgt accggccgag
      181 agcagcaccc cgcgttcgac cgcgtggtca agttctcgca cgttaccggg ccacagatgt
      241 tcgtgcagcg cggcggtggc cgcggagtcg aacccgccta tatttttgcg atagcgctga
      301 gaatatccgt gcacgaaatg gtgtgcaagc aggtcaatat cttcgcggcg ttctcgcagt
      361 ggcggaataa aaatctcaat agtgttcaag cggaacaaca ggtcctgccg aaaacgcccg
      421 gcggcggctt cggagtttac gtccacattg gtcgccgcaa gaacacgcac atcgacccgt
      481 cgagtggccg atgagcccac tcgttcgagc tcccccgttt ccagcacgcg caacaatttg
      541 gcctgcagat tgagcggtac gttggcaatt tcgtccagaa aaagagttcc gccgtcagca
      601 agttcgaatc gccctacgcg atctgatcgc gcatccgtaa acgcgccttt gacatgccca
      661 aaaagctcgc tttcgaagat tccttcagaa aggcctccag cgttcaccgt gaccatgggc
      721 ttggaagccc ggttagatag cgcatgcagc gtgcgcgcca caacttcttt gccggtaccg
      781 tgctcgccgg taatcagtac gtttgcgtcc gatgggccga tccgcgcgac cagatctagc
      841 accggtgcca tcgctggaga tttagcgagg aacgtgggac gtgattcggc gcgcagcagc
      901 cggttttctg cttccaaccg cgaagcttgc ctcaaagcct ggcgcaattc aatttgtgtg
      961 cgaacaatcg acagcagtcg attattctcc cagggctttt gcacgaagtc gcgcgccccg
     1021 cgccgcatgg cttccaccgc aatctccaca ctgctccacg cggtcatcac caccaccggc
     1081 aacgtactat ccagcatttg tatccgtgtg agcaggtcaa gcccttcctg cccggaagtg
     1141 gtgtcgcgcg cgtaattcaa atccatcagc acggcatcaa attcacgcga ctctatagcc
     1201 gcgagggcgt ccgcgggcga agccgcgccg tgcgtttcgt agccttcgcg cttcagcaat
     1261 aatcgcagag cgtcgcgaat gtcggattga tcgtcggcca gcaagacgcg ataggactgg
     1321 ttggcggcag atttcatttg atgaagaagt ttattccaat ttcgctattg agtgccaaga
     1381 acgcgaaggc gaaattgctg ccggcctgcg ccgggctatt gaaaactcat gcaaaagtta
     1441 ggccgattga accgttgcga tggcattttc gctgaccacc cagccatctt tcagttgcac
     1501 gatgcgattg ccgtaggcag cgttgcgctc cgagtgcgtg acctgaataa tagtcgtgcc
     1561 tgcatcgttc aattttttaa agagctgcat gatttcatcg ccctgcgcgg aatgaagatt
     1621 accagtaggc tcgtccgcaa gcagcaactt cggatgcaaa ataactgccc gcgcgacgcc
     1681 cacaagctgt tgctggcctc ccgaaagctg gttgggaaat aaatcctttt tccctacgat
     1741 gtgaaagcga tccagcgcat cgcacaccag gctcacccgt tccttatgag gaacattttt
     1801 atacgagagt ggcacttcga gattttcaaa aacggtcaaa ttgtcgatca aatgatagct
     1861 ctggaaaaca aacccaatgt tcgccttgtg cagttcattt cgcttcttgg cgtttaggtg
     1921 gtggacagcc tcgcccagga aaaagtactc gccagtccag gcgctgtcga gcatcccaat
     1981 aatgctaagc aacgtggatt ttccggcgcc ggaaggcccc atgatggtta caaattcgcc
     2041 ctgctgaatt tccaggttga tgcgccgcaa cacaaaaaac ttgctgccac cggcgtcgta
     2101 ggatttttcc aggcttctaa attggatcaa aggttccacg tcgctcccct tatcgaacgt
     2161 ctaggccgcg atcgcccaca acagccattt cgcgccacaa tcaaaccaaa attgtttcac
     2221 tcatagcgaa gcgccgtcaa cggatcgacg cgcacggcgc gccgagcggg gaggtaagac
     2281 gccaaggccg caattgcgcc gagaatcagc ggcagcccaa tgaaagaaaa gggatccgta
     2341 gccgacactc cgaaaagcaa gctcgacagc gcacgcgcca g
//
LOCUS       DATCBB010000019         1194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_454864, whole genome shotgun sequence.
ACCESSION   DATCBB010000019 DATCBB010000000
VERSION     DATCBB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454864"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>1194
                     /locus_tag="VII37_00245"
     CDS             70..>1194
                     /locus_tag="VII37_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEY5068172.1"
                     /translation="MEWLSSATRWLFGSEDGAADRLIPRWLFLRALGCIYFSAFFSLA
                     FQIRGLIGPNGILPANEYLQAVAHTFGHIRGVWFAPSVLWFSSGPHTLMALCWVGIGA
                     AVLLVMNLWPRRMLAICFVCFLSFVAAAQDFSGYQSDGMLLEAGFISLFFAPFGMRPG
                     FGRRSPPMRASLLLLQWEWFRIYFESGVAKIMSGDPEWRHFTAMDEYYQNGPLPTWIG
                     WYMQHLPHWFHASTAFATLALELVLVWMLFLPRRWRIVCFFIVTPWELGVIFTANYTF
                     LNYLVLALGVLLLDDEFLMRFVPQRWKGSVSGEATSEQRRTETPGRFRLHFKIAKRSV
                     VGVLFTWIFYATVAEMIWMVAPLHLPTSPVVALEPFRIANR"
BASE COUNT          199 a          288 c          356 g          351 t
ORIGIN      
        1 gtggaatcac ctgaacgatg attgccttgc aggtctcaac tcgcacgtat catataattg
       61 ctgacgatca tggaatggct ctcgtccgcc actcgatggt tattcggctc ggaagatggc
      121 gctgctgacc gcctgattcc gcgatggctg tttttgcggg cattgggctg catttatttt
      181 tctgcgttct tctctcttgc gtttcaaatc cgtgggctca tcgggccaaa tggaatactt
      241 cctgcgaacg aatacctgca agcagtagca cataccttcg gacacatacg aggcgtatgg
      301 ttcgcgcctt cggtgctgtg gttttcaagt ggcccgcaca ccttgatggc attgtgctgg
      361 gttggcattg gcgctgcagt gctattggtt atgaatctgt ggccgcgccg gatgctggcg
      421 atttgttttg tgtgcttctt gtctttcgtg gctgcagcgc aggatttttc aggttatcag
      481 tccgacggca tgctactgga agcgggcttt atctcgctgt tcttcgcgcc gttcggaatg
      541 cggccgggat ttgggcgcag gtcgccaccg atgcgcgcta gcctgttgct gctgcaatgg
      601 gaatggtttc gcatctactt cgagtcgggc gtcgcgaaga ttatgagtgg ggatccggag
      661 tggcggcact tcacggcgat ggacgaatat tatcagaacg gcccgctgcc cacgtggatc
      721 ggctggtaca tgcagcattt gccgcactgg tttcatgctt cgacggcgtt tgccacgctt
      781 gccctcgagt tggttctggt gtggatgtta tttttgccgc gccgctggcg gatagtttgt
      841 ttctttattg tgactccttg ggagctcggc gttattttca cggcgaatta cacttttttg
      901 aattacctgg tgctcgcgtt gggggtcttg ctgctggatg atgaattttt aatgcgattt
      961 gtgccgcagc gatggaaggg atctgtatct ggggaagcta catccgagca gaggcgaacg
     1021 gaaacccctg gacgttttcg acttcacttt aagatcgcca agcgatctgt ggtcggtgtt
     1081 ctattcacgt ggatttttta tgccacggtg gcggaaatga tctggatggt cgctccgcta
     1141 catctgccga cttcgccggt ggtggcgctt gagccatttc gcatcgcaaa tcgg
//
LOCUS       DATCBB010000020         3080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_422399, whole genome shotgun sequence.
ACCESSION   DATCBB010000020 DATCBB010000000
VERSION     DATCBB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422399"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1662)
                     /gene="guaA"
                     /locus_tag="VII37_00250"
     CDS             complement(43..1662)
                     /gene="guaA"
                     /locus_tag="VII37_00250"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY5068173.1"
                     /translation="MASTAKPVQDQAGRMEAELGGVVVLDFGGQYTQLIARRIREQQV
                     FSSILPCTTSMEELMKLEPVGVVLSGGPSSVYDPEAPKCDPKLLALGVPVLGICYGMQ
                     WITHTLGGKVERAERREYGRAQLNIENASGDGASDLFNGVAPSLRVWNSHGDHVMMLP
                     TGFRTVGRTANAVAAVEDRARKIYAVEFHPEVNHTEQGTEILRNFLFRVCKAEKKWSG
                     AAFIEETVEAIRKKAGPTSRVICALSGGVDSTVAAALVHRAIGDRLTNIFVNTGMLRK
                     NEFAETLEMLRERLGLRVIGIDASDRFLAKLEGVADPEVKRKRIGAEFISVFAEEAQR
                     LQREEAHGEIGFLVQGTLYPDVIESISVRGPSATIKTHHNVGGLPANMPFALIEPLRD
                     LFKDEVRRIGRELGLPEEILVKHPFPGPGLAVRLLGEVTRAHLLTLQEADAVVVEEIR
                     RAGLYEKVWQAFAVLLPVRSVGVMGDGRTYGLTVAVRVVQSDDAMTADWVRLPGDVLE
                     RISTRIVNEVPGVTRVVYDISSKPPSTIEWE"
     gene            complement(1663..>3080)
                     /locus_tag="VII37_00255"
     CDS             complement(1663..>3080)
                     /locus_tag="VII37_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5068174.1"
                     /translation="VQPRDGEALNNEKLKEAFTEYGKSVNSGPYSGLTTEEALDRMSA
                     EAEAKGFGKRETVFRLKDWGISRQRYWGTPIPVIYCPKDGMVAVADADLPVLLPENVK
                     LTGEGESPLAATPEFVNVKCPKCRGDARRETDTMDTFVDSSWYFYRYCDPHNDQAAFD
                     KAKVEYWFQIDQYIGGITHAILHLLYSRFWCKVMRDMGLVTHSEPIARLFTQGMVQKG
                     GVAMSKSKGNVVGAEEMADKYGADTGRLYTLFAAPPEKDLEWSEESIEGSWRFLNRVY
                     RLVERHAEAVRAVKSESSGAGTKAIAPSSAKEKQLLRKTHQTLRRVTQDFETRWHFNS
                     AIALIMDLTNEIYALEPLGEGVRPEVQKDVLELLTLMLAPMTPHIAAEMWEMLGHEDG
                     QWTAAWPVFNADLARETEVEIVVQVNGRVRGKLKVAAGMPEEEVVALAKSEPNVAPHL
                     DGKRVVKHIFVPDKLVNLVVA"
BASE COUNT          628 a         1090 c          650 g          712 t
ORIGIN      
        1 ttaattttct gaattaattt cccacgaggt gcttccaaga ctttattccc actcaatcgt
       61 gcttgggggt tttgagctaa tgtcgtacac cacccgcgtt actcccggaa cctcattgac
      121 gattcgcgtc gagattcgct ccagcacgtc cccgggcaat ctcacccaat ccgccgtcat
      181 ggcatcgtca ctttgcacca ctcgaaccgc aacagtcagg ccgtatgtcc gtccgtcgcc
      241 catcactccc acgctgcgta ccggcaaaag caccgcgaag gcctgccaca ctttctcgta
      301 caatcctgct cgccggatct cttccaccac caccgcgtct gcttcctgaa gagtcaacaa
      361 atgcgcccgg gtcacttctc ctagtaaccg aactgccaat ccaggacccg gaaacgggtg
      421 tttcactaaa atctcttccg gcaaccccag ttccctgcca attcgccgca cctcatcctt
      481 aaacaggtcc cgcagcggct caatcaacgc aaatggcata ttggccggca ggccacccac
      541 attgtgatgg gtcttgatcg tcgccgaagg cccccgcacc gagatcgatt caatcacgtc
      601 gggataaagc gtcccctgca ccaggaaacc aatctcccca tgggcctctt cccgctgcag
      661 ccgttgcgct tcttccgcga atacagaaat aaattcggct ccgatccgct tgcgctttac
      721 ctctgggtcc gcaacaccct caagcttcgc cagaaatctg tccgacgcat cgatgccaat
      781 cactcgcagg cccagccgct ctcgcagcat ctccagagtt tccgcgaact cgttcttccg
      841 cagcatgccg gtgttcacaa aaatatttgt cagccggtcg ccaatcgccc gatgcaccag
      901 cgccgccgcc actgtcgagt ccacgccgcc gctcagcgca caaattactc tgctcgtcgg
      961 ccccgctttc ttgcgaatag cctccaccgt ctcttcgata aacgccgcac cgctccactt
     1021 cttctccgct ttacacacgc gaaacaaaaa gttgcgaagt atctccgtgc cctgttcggt
     1081 gtgattcact tccggatgaa actccaccgc ataaatcttg cgcgcacggt cttccaccgc
     1141 cgccaccgca ttggcggtcc gccccaccgt gcgaaatccc gtcggcagca tcattacgtg
     1201 atcgccgtga ctgttccata ccctcagaga gggcgccacg ccgttaaaca aatccgacgc
     1261 gccgtcgccg ctcgcattct caatattcaa ttgcgcgcgc ccatactctc gccgttctgc
     1321 acgctccacc ttaccgccca acgtgtgcgt aatccactgc atcccgtagc aaatccccaa
     1381 caccggcacg cccagcgcca ataatttagg gtcgcacttc ggagcttccg ggtcatacac
     1441 ggagctcggc ccgcccgaca aaaccacgcc gacaggctcc agcttcatca gctcttccat
     1501 ggatgtggtg cacggcaaaa tcgacgaaaa cacttgttgc tcgcgaatcc gccgcgcaat
     1561 caactgcgtg tactgcccgc caaaatcaag caccaccacg ccgccaagct ccgcttccat
     1621 cctgcctgcc tgatcctgta ctggttttgc tgtgcttgcc atttacgcca ccaccaggtt
     1681 tacgagtttg tccggcacga aaatatgttt taccacgcgt tttccatcga ggtgcggcgc
     1741 cacgttgggt tcagacttcg ccagtgcaac cacttcttct tccggcatcc ccgcggctac
     1801 ctttaatttt ccacgcacgc gcccgttcac ttgcaccaca atctcgactt ccgtctcacg
     1861 cgccaggtcc gcattaaaca ccggccacgc ggcggtccat tggccatctt catggcccag
     1921 catttcccac atttccgccg cgatatgcgg cgtcatcggc gccagcatca gcgtcaataa
     1981 ttccagcacg tctttctgca cttccggtcg cacgccctca ccgagtggtt ccagggcata
     2041 aatctcgttg gtcaaatcca tgatcaacgc aattgcagag ttaaaatgcc agcgcgtctc
     2101 gaaatcctgc gtaacgcgcc ttaatgtctg atgtgtcttg cgcagcaatt gcttttcttt
     2161 cgcggaactc ggcgcgatcg cctttgtgcc cgccccgctg ctctcgctct tcaccgcgcg
     2221 cactgcttct gcatgccgtt ccaccaggcg atatacacga ttcagaaatc gccatgatcc
     2281 ttcaatactt tcttcgctcc actccaaatc cttttccgga ggtgccgcga ataacgtgta
     2341 cagcctcccg gtatccgcac cgtatttgtc tgccatctct tcggcgccca ctacattgcc
     2401 ctttgacttg gacatggcca cgccgccctt ttgcaccatg ccttgcgtaa aaagcctcgc
     2461 aattggttca ctgtgcgtga ccaatcccat atcgcgcatc accttgcacc agaatcgcga
     2521 atacagcagg tgcaaaatcg cgtgcgtaat cccgccaata tactggtcga tctgaaacca
     2581 gtactccacc ttggccttat caaacgccgc ttggtcattg tgcggatcgc aataacgata
     2641 gaaataccac gacgaatcca cgaacgtatc catcgtgtcc gtttcccgcc gcgcatcgcc
     2701 gcggcacttc gggcatttca cattcacgaa ttccggcgta gccgccagcg gcgactcccc
     2761 ttcgcctgtc agtttcacat tttcgggcag caacaccggc aaatccgcat ccgccaccgc
     2821 caccattcca tctttcggac agtaaattac cggaatcggc gtgccccaat atctctgccg
     2881 cgatattccc cagtccttca accgaaagac tgtctcgcgt ttgccaaatc ccttcgcttc
     2941 cgcctccgcc gacattcgat cgagggcctc ttccgtcgtc aatccgctgt agggccctga
     3001 attcacgctc ttgccgtatt cagtaaaggc ttcctttaat ttctcgttgt ttagtgcctc
     3061 accatctctc ggttgcacca
//
LOCUS       DATCBB010000021         1740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_390030, whole genome shotgun sequence.
ACCESSION   DATCBB010000021 DATCBB010000000
VERSION     DATCBB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390030"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VII37_00260"
     CDS             <1..1726
                     /locus_tag="VII37_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY5068175.1"
                     /translation="EKQLKQSAALDFDDLLLRTVRLLRESPQVREKWQARFQYILVDE
                     YQDTNRVQYELLRLLTGPKHNLCVVGDEDQSIYRWRGADVSILLSFSRDFPDAKVIKL
                     ERNYRSTQQILNAAGAVVANNPERLGKTLRAENGEGSNLRYFEARDAQGEAEFVAGEL
                     ERIIGDDPSQTCAVEYRTNFQSRSFEEAFRRRGLRYKLVGGFSFYNRAEVKDTLAYVR
                     LAMHPDDDVSLLRVLNVPPRGIGKTTLDALRETARADGSSLWTAIGKLISGANGGRAV
                     TPLRVFQEMIGKLQSALSEQDHADFLRTVLDETGYMDMLKDRNTPDDVARIENLEELT
                     RAVAESMDAGETFADFLDAAALVSDADSFEGKPGVTLITLHSTKGLEFDHVFLTGLEE
                     GICPHSRSLNEEKGIEEERRLCYVGMTRARKSLTLSRAVYRRVFGNEQQMRASIQSRF
                     LTEIPSELVNTVRGSMSEIGEKRRYELDPEYSYSSEEFLQRVRSGMKPVRSPLRQSAP
                     SSFTRPPLKRGADANPMLGRKVRHPTYGTGTVVGVEGDDDDRRVSVSFPGRGTKKFIE
                     RYAQLELA"
BASE COUNT          407 a          408 c          551 g          374 t
ORIGIN      
        1 cgagaaacag ttgaaacaaa gcgcggctct ggacttcgac gacctgctgc tgcggacggt
       61 aaggctgctg cgcgaatctc cgcaggtgcg tgagaagtgg caggcgcggt tccaatacat
      121 tcttgtcgac gagtatcaag acaccaatcg cgtgcaatat gagttgctga ggctgctgac
      181 cgggccgaaa cacaatctgt gcgtggtggg cgacgaagat cagtcgattt atcgttggcg
      241 tggggcggac gtttcgattc tgttgagttt ttcgcgcgat tttccggacg ccaaggtcat
      301 caagctggaa cggaattatc gctcaacgca gcaaatcctg aatgccgctg gcgcggtggt
      361 tgccaacaat cctgaacggc tgggcaaaac gcttcgcgcc gagaatggcg aaggctccaa
      421 cctgcgctat ttcgaagcgc gtgacgcgca gggcgaagcg gaatttgtgg ctggcgaact
      481 ggagcggatt ataggcgatg acccttccca gacgtgtgcg gtcgaatacc gaaccaattt
      541 tcaatcacga tcgtttgaag aagcgtttcg acggcgggga ttgcgataca aattagtggg
      601 aggattcagt ttttataatc gcgccgaggt gaaggatacg ctcgcgtatg tgcggctggc
      661 gatgcatccg gatgacgatg tgtcgctgct tcgggtacta aatgttccgc cgcgcggtat
      721 tgggaaaaca actttagacg ctttgcgcga aacagcccgg gcggacgggt cgtcgttgtg
      781 gacagcgatc ggcaagctta tttcgggtgc caacggcggt cgcgcggtta cgccattgcg
      841 tgtatttcag gaaatgatcg gcaaattgca atctgcgcta tcggaacaag atcatgcgga
      901 ttttcttcgt actgttctgg atgaaactgg ttacatggac atgctgaagg atcgcaatac
      961 tcctgacgat gtggcgcgca ttgagaacct cgaggaactg actcgcgcgg tcgccgaaag
     1021 catggatgcg ggcgaaactt ttgcggattt tctggatgcc gcggcattgg tgagcgacgc
     1081 tgattctttc gaaggcaagc cgggcgtcac attaattacg ctacatagca cgaaggggct
     1141 ggagttcgat catgtgtttt tgacggggct ggaagaaggc atatgcccac acagccgctc
     1201 gttaaacgaa gagaaaggta tcgaagaaga gcggcgactc tgttacgtgg gaatgacgcg
     1261 tgcgcggaaa tcgctgacgc ttagccgcgc agtgtaccgg cgcgtttttg ggaatgagca
     1321 acaaatgcga gcgtcgatac agtcgaggtt tctaacggaa attcccagcg agctcgtaaa
     1381 taccgttcgc gggtcaatgt cggaaatcgg cgagaaaagg cgttacgagc tggacccgga
     1441 atattcttac tcgtcggaag aattcttgca gcgagtacgt agcgggatga aaccagtgcg
     1501 atcgccgctg cggcaaagtg ctccgagctc ttttacacgg ccaccgctaa agcggggagc
     1561 ggatgcaaat ccgatgctag gacggaaagt gcggcatccg acgtacggta ccggaacagt
     1621 tgtgggcgtg gaaggcgacg atgacgatag gcgggtgtca gtgagtttcc cgggccgcgg
     1681 cacgaaaaag tttattgagc gctacgcgca actcgaactg gcataagatt tacgcccagg
//
LOCUS       DATCBB010000022         1248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_33234, whole genome shotgun sequence.
ACCESSION   DATCBB010000022 DATCBB010000000
VERSION     DATCBB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33234"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VII37_00265"
     CDS             complement(<1..761)
                     /locus_tag="VII37_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5068176.1"
                     /translation="MSTEVSVPLANGMEGDRPHLRRVLGRWDLVLLFVVAVFNLNVVP
                     SIAANGGVTVWLWLISLVLFFWPQGIAVIELAHRFPGEGGVYLWAKEVFGDFHGFLSG
                     WCYWTNNMMYVPTVMLYFVGVSVYALGAGHEALADNKTFALSASLLLLIILVVMNIVG
                     LGVGKWINNVGAIGTGIAAVVLIGLGVVVWLRFGTTVTSSDFAIPANPRFVLNSFGVI
                     CFGLVGLELASVMGDEIENPERTLPGAVVWGGVLSG"
     gene            complement(791..>1248)
                     /locus_tag="VII37_00270"
     CDS             complement(791..>1248)
                     /locus_tag="VII37_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068177.1"
                     /translation="FHGGTYNTNRISMAAGLATFREVLTRENYEHVGKLSQKLFEGYR
                     KTVAKVGLQAYVVSAGVNGALMLYPQEIRNYRDWTAIDVDLWRHYWFAMVNRGVLAQP
                     YWWDEQWTISVQHTEADIDKHLAVFEEIAPALAKAQQERGVAFVGAAGH"
BASE COUNT          295 a          455 c          279 g          219 t
ORIGIN      
        1 ccggaaagca cgccgcccca caccaccgct cccggcaaag ttctctccgg attctcgatt
       61 tcgtcgccca tcaccgacgc cagctccagc cccaccagcc caaagcagat caccccaaac
      121 gaattcagca cgaatcgcgg attcgccgga atcgcaaaat cgctggaggt caccgtcgtt
      181 ccaaatcgca accacaccac cacgcccaag ccgatcagca ccacggcggc aatccccgtg
      241 ccgatggcgc cgacattgtt gatccacttg cctacgccca gccccacgat attcatcacc
      301 accagaataa tcagcagcag cagcgacgcc gacagcgcaa aagttttatt gtccgccagt
      361 gcttcgtgcc ccgcgcccag cgcgtatacc gacacgccca cgaaatacag catcaccgtg
      421 ggcacgtaca tcatgttgtt cgtccaataa caccaccccg acaaaaatcc gtggaagtcc
      481 ccgaaaactt ccttggccca caaatacacg ccgccttccc cgggaaagcg atgcgccagc
      541 tcgatcaccg cgatgccctg cggccagaaa aacagcacca gcgaaatcag ccacagccat
      601 accgtcactc cgccgttggc tgcaatggat ggaactacgt tgagattgaa aaccgcgacg
      661 acaaaaagta aaacgaggtc ccatcgcccc agtacacgcc gcaggtgcgg cctgtcgcct
      721 tccatcccat tcgcaagcgg cacgctgact tccgtcgaca tagtttcccc gccgcgcgat
      781 cgctgaaaac ttagtgcccc gcagcgccca caaacgccac gccgcgttcc tgctgcgctt
      841 tcgccagagc gggagcgatt tcttcaaaca ccgcgaggtg cttatctata tccgcttcgg
      901 tgtgctgcac cgaaatggtc cactgctcgt cccaccaata aggctgcgcc agcactccgc
      961 gattcaccat cgcaaaccag tagtgccgcc acagatccac atcaatcgcc gtccagtcgc
     1021 gataattgcg aatctcctgc ggataaagca tcagcgcgcc attcactccc gcgctcacta
     1081 cgtaggcctg caagcccacc tttgccaccg tcttgcggta gccctcgaaa agtttttggc
     1141 tcaacttccc gacgtgctcg taattctcgc gcgtcaacac ttcgcgaaaa gtcgccagcc
     1201 ccgcggccat cgagatgcgg ttggtgttgt acgtccctcc gtgaaaaa
//
LOCUS       DATCBB010000023         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_227865, whole genome shotgun sequence.
ACCESSION   DATCBB010000023 DATCBB010000000
VERSION     DATCBB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227865"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>2321
                     /locus_tag="VII37_00275"
     CDS             15..>2321
                     /locus_tag="VII37_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY5068178.1"
                     /translation="MSWDGKIAGPSGKPTFSGHVRGENVQYSSIILDSLDGDLVYSPG
                     ELLLSRGHARRGSTSSSLDVNLELTDWSFLPHNSWSADASLEKTSLEGLQQLAGWSYP
                     VRGNLTGQFHGRGTRNDPTITGLFDLADGNVYGSSFNRLRGQLTASTQEVRIANAELR
                     FFATGNEKGRGAGIVTGTAAYRFTDETISADLVGAALPLESFDKLQSSIALGGQVNFR
                     LKAEGPVQAPTANGTFRVTDLRVGQEVIGSFDTDLVSDGKTARLTLGSAMSTGQISGG
                     YTLGLANPFPVEGKVSIQNIDLDPFLLAALHLKQFSGHGQADGDISLKGALKDPRGIV
                     VEANFSRLVLNYANVRLENNGPVRFRSSREALEIEPVIMHGTDTNVKIDGSVQFTGRR
                     NLALHLDGAVDLRLLNVSVPNMDVHGGAQINAAVEGTIDHPRITGRVHIDNASVRAAD
                     FPTGLSAIKGDLIFDTTRLFFSDVTAEVGGGNLQLSGSVNYGEGGMRYDLNTSTDRVR
                     IRYPEGMSWLLGGNLRLAGTTNSGVVSGKVTVQRVLLTQGLESAGALVSSKEGISGPT
                     TDSSFLRNLQFDIEAASTPDARMEWPGAELEADANLRVRGTWEHPILLGHIHLLSGDL
                     YFAGNRYRVARGDINFANPFRLDPVVNVEASTTVQQYEVTLNFNGPSSKLALAYRSDP
                     PLPSNDIVALLAMGQRSAEGTARSGGTAQNATSGATALLSEAVSNQLGGRLQRLFGIT
                     RLRVDPGLTPIASSSDQNAGARVTVEQQI"
BASE COUNT          497 a          660 c          662 g          502 t
ORIGIN      
        1 ctctatcagg aaaagtgagc tgggacggaa aaatcgccgg cccgtccggg aagccgacat
       61 tctctggaca tgtgcgcggc gagaacgtgc agtacagttc gatcattctg gattcgctcg
      121 atggcgactt ggtgtattcg cccggggagt tgttgctctc gcgagggcac gcgcgccgcg
      181 ggtccacaag cagttccctc gatgtcaatt tggaactcac tgactggagt tttcttcctc
      241 ataattcctg gtctgccgac gccagcctcg agaaaacttc gctcgaaggt ctgcagcagc
      301 ttgccggctg gtcctatcca gtgcgaggaa atttaacggg ccaattccac ggccgtggca
      361 caaggaacga cccgaccatt accggccttt tcgatttggc cgacggaaat gtttacggct
      421 catctttcaa tcggctgcgc gggcaactta ctgccagcac tcaggaagtt cgcattgcca
      481 acgccgaact acggtttttc gcgacaggaa acgaaaaggg acgcggagcc ggcattgtca
      541 ccggaactgc agcctaccgt tttacggacg aaacaatttc cgccgattta gtcggcgcgg
      601 cgctcccact cgaaagtttc gacaagttgc aatcttcgat tgccttgggc ggtcaagtga
      661 attttcggtt gaaggctgag gggccagtcc aggcgccgac tgcaaatggc acattccgcg
      721 tgacggatct gcgtgtgggg caggaagtga tcggcagttt tgacaccgat ctggtctccg
      781 acggaaaaac agcacggttg acgctgggtt ctgcgatgag caccggacag atttctggcg
      841 gttacacctt ggggctcgcg aacccatttc cggtagaagg aaaagtctcc atccagaata
      901 tcgatctcga cccgttcctg ctggctgcgt tgcatttaaa gcaattcagc gggcatggac
      961 aggccgacgg cgatatctcg ctgaaaggcg cgttgaaaga tcctcgcgga atcgtggtgg
     1021 aggccaattt ctcgagactg gttctcaact acgcgaatgt gcggcttgag aataacgggc
     1081 cggtgcgttt ccgctcctcg cgcgaagcgc tcgagattga gcctgtaatc atgcatggta
     1141 ccgacaccaa cgtgaagatc gatggatccg tacaatttac cggccggcga aatcttgcgc
     1201 tccatctcga cggcgcggtc gatctgcgcc tcctgaacgt ttccgtgcca aacatggatg
     1261 tgcatggcgg tgcgcaaatt aatgccgccg tggaaggcac catcgaccat ccgcgcatca
     1321 ctggacgagt gcacattgat aatgcctcgg tgcgcgccgc ggattttcct accggcctga
     1381 gcgccatcaa aggcgacttg atttttgata ccactcgcct tttcttcagc gacgtaaccg
     1441 ccgaagtggg aggcggcaac ctgcagctct ccggcagcgt aaattacggt gaaggcggaa
     1501 tgcgctatga cctcaacacc agcaccgacc gcgtgcgcat tcgctacccg gaaggtatga
     1561 gctggctact gggtgggaat ttacgactgg ctggcacgac caactctggc gtcgtttccg
     1621 gaaaagtcac cgtgcaacgg gtgctgttga cgcagggcct cgaaagtgcc ggagcattgg
     1681 tttcgtcgaa agaaggcatc tccgggccta caacagattc ttcgttcttg cggaatttgc
     1741 agttcgacat tgaagccgca tccactcccg acgcgcgcat ggaatggccc ggcgcggaat
     1801 tggaagcgga cgccaatttg cgagtgcgcg ggacctggga gcatcccatt ttactgggac
     1861 acatacacct gctttcaggt gatttgtact ttgcggggaa tcgctatcgc gtagcacgcg
     1921 gcgatattaa tttcgcaaac cctttccggc tggaccctgt ggtcaacgtg gaagcctcca
     1981 ctaccgttca acaatatgaa gtgactctca acttcaacgg gccatccagt aaacttgcgc
     2041 tcgcctatcg ctccgatcct ccgttgccct ccaatgacat cgtggcgctt ttggcgatgg
     2101 gacaacgcag cgcggaaggc accgcgcgca gcggcggcac ggcacaaaat gccacttccg
     2161 gcgcgacggc acttttgtca gaagcggtgt ccaatcaact cggtggccgg ctgcaacgtt
     2221 tattcggaat taccaggttg cgcgtggatc ctggcctaac ccccattgcc tccagttccg
     2281 atcaaaatgc cggcgcgcgc gtcaccgtgg aacaacaaat c
//
LOCUS       DATCBB010000024         5289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_130617, whole genome shotgun sequence.
ACCESSION   DATCBB010000024 DATCBB010000000
VERSION     DATCBB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130617"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VII37_00280"
     CDS             <1..715
                     /locus_tag="VII37_00280"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY5068179.1"
                     /translation="LLQFRAWRPTQHGLETHLLRIHDLVDQFNPQVVIVDPIINLISA
                     NLNEVHAMLMRLLDFLKDRQITAMFTTLTANRGAEEQTEVGISSLTDTWILLRDLELN
                     RERNRCLYVLKSRGMAHSNQIREFILSRNGVRLLPAYVGSGTVYTGSARLAQEARERA
                     EALREQQQLEERRKEFSGKRSVLESQIAALRSELSTGEAEFARLTRQRNERHQRLALD
                     REEMGKRRGVTPANQGLNS"
     gene            712..1053
                     /gene="kaiB"
                     /locus_tag="VII37_00285"
     CDS             712..1053
                     /gene="kaiB"
                     /locus_tag="VII37_00285"
                     /inference="COORDINATES: protein motif:HMM:NF006798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiB"
                     /protein_id="HEY5068180.1"
                     /translation="MRTSKVKSKPLKTEATGNATNAILELRLYVAGQTPKSLVALKNL
                     KKICEDHLQGRYCLHVIDLVKTPQLAQDDQILAIPTLVRNLPQPIRKIIGDLSDTQRV
                     LVGLDIRSEAK"
     gene            1064..1465
                     /locus_tag="VII37_00290"
     CDS             1064..1465
                     /locus_tag="VII37_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEY5068181.1"
                     /translation="MTAKAKRDSKIKRNKSAIKARKRSASAAQKKAAKRSGSRYALRL
                     YITGQTPRSLQSVENLRALCDKYIPNKFDLEVVDIYQQPAMAAAGQIIAAPTLIKSMP
                     LPLRRLVGDFSDQNRVILGLDIRVDEEQGSA"
     gene            1458..3143
                     /locus_tag="VII37_00295"
     CDS             1458..3143
                     /locus_tag="VII37_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5068182.1"
                     /translation="MPKRKKRPASSIQVTELLARIAELEETLRAIRMGEVDAVLVSGP
                     QGDQVFTLQGAEHPYRLMVETIDEGAATLAEDGTVLYANRSFAEIFDVPLEKFIGAPL
                     NDFVVGEDRELLAVLIADANLNIVRGEIRLDSHRQRPRTIRLTLSPVREQGVHTICVV
                     ATELTELIETNEALRVSELSLRQLSARLLKLQDEERRRIARDLHDTTGQKIAVLSMTL
                     DRLAKLVDTRKVDVTDALTESREVVGKIGEEIRTLSYLLHPPLLDECGLASAVLWYAE
                     GFKKRSGIHLNVSIQEDLVRLPTDAETALFRVLQESLTNVHRYSGSPSAEIHIFQSAN
                     KVHLEIVDHGKGVRAGTEPSSFAGAPTLGVGIPGMRERIRQLGGQLEVEFSNEGTRVH
                     ASLPMAGAAEEPVAQSSDELFRDKENFQANPRQRPDVRKRILIADDHEVMRRGVRGLV
                     ESQEEWSVCGEAIEGKEAITKTRELRPDLLILDVSMPGMSGIEAALQILKDDPGTKIL
                     FFTMHDSPQMMREISNVGAWGYVAKARAGNDLVDAVRIILDGKKFFPRIASAS"
     gene            3393..3962
                     /locus_tag="VII37_00300"
     CDS             3393..3962
                     /locus_tag="VII37_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY5068183.1"
                     /translation="MTDKQLNGLRVAILATDGFEEAELLEPRKALDAAGAKTTVIAPK
                     SGKIQGMKHDEKSEKVAVDAIFDNANPADFDAVLLPGGALNADALRVVRPAQQFVQEA
                     NNARKPIAFICHAPWLLVSAGLTKGRTLTSYHTIQDDLRNAGAKWVDKEVVRDGNFVS
                     SRQPTDIPAFNREMISLFSEPKAKVRSAS"
     gene            4123..5115
                     /locus_tag="VII37_00305"
     CDS             4123..5115
                     /locus_tag="VII37_00305"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY5068184.1"
                     /translation="MSHRIAAKITGVAGCVPPKILTNADLEKMVATNDEWIRTRTGIR
                     ERHIVEKGTPTSHLAVQAAKTLLAQTKTDPAEIDLIVLATVTPDMMFPATACLVQNQL
                     GATKAWGFDLSAACAGFAYALTVGSQFAAGAHKKVLVIGSDTMSSIIDYKDRGTAILF
                     GDGAGAVLLEPAGDNEGILDFSHDVDGSGGNFLYMPGGGSLNPPTHDTVDKNMHVVHQ
                     EGSQVFKYAVRRMAELAVNLLERNGFKSDDLALVVPHQANLRIIRAMQERLGVDDSKV
                     MVNIDRYGNTTAGTIPLGLRDAVAEGRLKKGDLVLLVAVGAGYTTGGVLMRWAY"
BASE COUNT         1239 a         1614 c         1317 g         1119 t
ORIGIN      
        1 tctattgcag tttcgcgcct ggcgtcccac gcaacatggt ctcgaaacgc atttgctgcg
       61 cattcacgat ctggtcgacc aattcaaccc tcaagtggtt atcgttgatc ccatcatcaa
      121 tctcatcagc gccaatctca acgaagtcca tgccatgctc atgcgcctcc tggactttct
      181 gaaagatcgc cagatcaccg ccatgtttac caccctcacc gccaaccgcg gcgctgaaga
      241 acaaaccgaa gtaggcattt cctccctcac cgatacctgg atacttcttc gcgacctgga
      301 gctaaaccgc gaacgcaatc gctgcctcta cgttttaaaa tctcgaggaa tggcgcactc
      361 taaccaaatc cgcgaattta tcctaagccg caacggagtt cgcctgcttc ccgcgtacgt
      421 cggatcgggt accgtctaca ccggttccgc gcgtcttgca caggaagccc gcgaaagagc
      481 tgaggcgctc cgcgagcagc agcagttgga ggagcggcgc aaggaatttt ccggcaaacg
      541 tagtgttctg gagtcgcaga tcgccgcgtt gcgttccgaa ttgtccactg gcgaagccga
      601 atttgcgcgc ctcacccggc aacgcaatga acgccaccaa aggttagccc ttgatcgcga
      661 ggaaatgggc aagagacgcg gcgtcactcc agccaatcag ggcttgaact catgaggacc
      721 tccaaggtga aatcgaaacc tctaaaaaca gaagccactg gcaacgctac aaacgccatc
      781 ctcgagctgc gcctttacgt tgccggtcaa acgccaaaat ctttggtcgc cctgaagaat
      841 ctaaagaaaa tttgtgagga tcatctgcaa ggccgttatt gcctgcacgt catcgacctg
      901 gtgaaaacac cgcaactcgc ccaggacgat cagatactcg ctattcctac cctcgtgcgc
      961 aatcttccgc agcccatccg caaaattatc ggcgatcttt ctgataccca gcgtgtcctt
     1021 gtcggcctcg atattcgttc ggaagcgaaa tagtgcaacc gccatgacag ccaaagcaaa
     1081 acgcgattca aaaattaagc gaaataaatc agcgattaag gcaagaaagc gatccgcgtc
     1141 cgccgcgcaa aagaaagccg caaagcgctc cggttcccgc tacgctctgc gtttgtacat
     1201 caccggccaa actccccgct cgctgcagtc cgttgaaaat cttcgcgccc tgtgcgacaa
     1261 atacattccc aacaagtttg accttgaagt cgtagatatc tatcagcaac cagccatggc
     1321 tgcggcgggc cagatcatcg ccgcgcctac cctgattaag tccatgccct tgccattgcg
     1381 tcgcctggtc ggcgatttct ccgatcagaa tcgtgtgatt ctgggcctgg atatcagagt
     1441 ggatgaggag cagggtagtg cctaagcgga aaaaacggcc ggccagctct attcaggtta
     1501 ccgagctgct tgctcgcatc gcggaactgg aagaaaccct tcgtgccatc cgcatgggcg
     1561 aggttgacgc tgtgctcgtc tccggcccgc aaggcgatca agtcttcact ctccagggtg
     1621 ccgaacatcc ctaccgcttg atggttgaaa ccatcgacga aggcgctgca accctggccg
     1681 aggacggcac cgttctgtat gccaaccgca gtttcgccga aatttttgac gttcccctcg
     1741 aaaagttcat cggcgccccg ctgaatgatt ttgtggtcgg cgaagatcgc gaactgctgg
     1801 ccgttctcat cgcggatgct aatttaaata ttgtgcgcgg cgaaattcgc ctcgatagcc
     1861 accggcagcg cccccgcacc attcgtttaa ctttaagccc ggttcgcgag cagggcgtac
     1921 acaccatctg cgttgtcgcc acagaactca cggagttgat cgaaaccaac gaagccttgc
     1981 gcgtcagcga actttccctc cgtcaactct ccgcgcgcct cttgaagctc caggacgaag
     2041 agcgccgccg catcgcccgc gatctgcacg acaccaccgg tcaaaaaatt gccgtgctct
     2101 ccatgaccct cgatcgtctc gcgaaactcg tcgacacccg caaagtcgat gtcacggacg
     2161 ccttgaccga gtctcgtgag gtggtcggca aaatcggcga ggaaattcgc actctctcgt
     2221 atctcttgca tccgccgctc ctcgatgaat gcggtctcgc ttccgcggtg ctctggtacg
     2281 ccgaaggttt caaaaagcgc agtggcatcc atctgaatgt atccatacag gaagatctgg
     2341 taaggctccc caccgatgca gagaccgctc tgtttcgtgt gttgcaggaa agcctgacca
     2401 acgtgcaccg gtactctggc agccccagcg ccgaaataca tattttccaa tcagccaaca
     2461 aggtgcacct ggaaattgtc gatcacggaa aaggcgtgag ggccggcacg gagccctctt
     2521 cctttgccgg cgcgccaact ctcggtgtcg ggattcctgg catgcgcgaa cgcatccgac
     2581 agttgggtgg ccagctcgaa gtggaattta gcaatgaagg aacgcgcgtg cacgcttcgc
     2641 tacccatggc aggcgcagca gaagaacccg tcgcgcaatc atcagacgaa ctttttcgcg
     2701 ataaggaaaa ttttcaagcc aatcctcgcc agcgccccga tgttcgcaaa cgcatcctca
     2761 tcgcggacga ccacgaagtt atgcgtcgcg gcgtgcgcgg cctggtggaa agtcaggagg
     2821 aatggtcggt ttgcggcgaa gccatcgaag gaaaggaggc catcaccaag accagggaac
     2881 tgcgccccga cctcttgatc ctggacgtca gcatgcccgg aatgagcggc atcgaagcag
     2941 ccctgcaaat ccttaaggat gatcccggca caaaaattct cttcttcacc atgcatgatt
     3001 ctcctcagat gatgcgtgag atttccaatg taggcgcctg gggttacgtc gccaaagcgc
     3061 gcgccggcaa tgacctggtt gacgccgtcc gcatcattct cgacggaaaa aaattctttc
     3121 cccgtatcgc ctccgcatcc tgaaactgga aagccattca ctcgaacggc gccgaaatcc
     3181 tcttttcgca ttagtatttg cgaaatagac tcacgttttt ctcaattgtt aacgatgtta
     3241 agaatctttt tatttttcgt atttttttcc tacagccttt gcccgattgc accgtcgtcg
     3301 ttgtagacca accataacag tggagcgagt cttgctaagt tgccgcatga actcacgaaa
     3361 ttgctccaat ctaaaataag tcgaggaacg ttatgactga taaacaatta aacggattac
     3421 gcgtagccat tctggccacg gatggatttg aagaagccga attgctggaa ccccgcaaag
     3481 ccctggacgc cgcgggcgca aagaccaccg tgatcgctcc caagagcggc aagattcaag
     3541 gcatgaagca cgacgaaaaa tcggaaaaag ttgccgtaga cgctatcttt gacaatgcca
     3601 accccgccga tttcgacgcc gtgctgctcc ccgggggtgc cctgaatgcc gacgcgcttc
     3661 gtgtggtgcg acccgcgcag caattcgtac aggaagctaa caacgcccgc aagcccatcg
     3721 cttttatctg tcacgccccg tggttgctgg tctctgccgg cctgaccaaa ggccgcacgc
     3781 taaccagcta ccacaccatt caggatgacc tgcgcaatgc gggcgccaag tgggtggaca
     3841 aagaagttgt gcgtgacggc aatttcgtca gcagccgtca acccaccgac atccccgcct
     3901 tcaatcgtga aatgatctcg ctcttcagcg aacccaaagc taaggtgcgc tccgcatcct
     3961 aaaacttcag ccgctaaaaa ccccgtggtt cattagcccc ggggttttct ttttccatca
     4021 acaaatgaaa ccgcgggttt tctgcgctca ctccttgtaa ctccgctccg ctatcgcata
     4081 aaattcataa tcttctgacc gggtcattcg tgaaggagtt tcatgtctca tcgcatcgcc
     4141 gcaaaaatta cgggtgtggc aggttgcgtt cctcccaaaa tcctcaccaa tgccgacttg
     4201 gaaaaaatgg tggccaccaa cgatgagtgg atccgcaccc gcactggcat ccgcgaacgc
     4261 cacatcgtag aaaagggcac acccacttcg catctcgcgg tgcaagccgc caagaccctt
     4321 ctcgcacaga ccaaaacaga tcccgctgaa atcgatttga tcgtcctcgc caccgtcact
     4381 ccggacatga tgtttcccgc caccgcctgc ctcgttcaaa atcagcttgg cgccacgaaa
     4441 gcctggggct tcgacctctc cgccgcctgc gcaggtttcg cctacgccct caccgtcggt
     4501 tcccagttcg ccgccggcgc gcacaaaaaa gttctagtca tcggcagcga caccatgagc
     4561 tccatcatcg attacaaaga tcgtggcaca gccattctct tcggcgacgg cgctggcgca
     4621 gttttactcg agcccgccgg cgacaacgag ggcattctcg atttctctca cgacgtggac
     4681 ggttccggcg gaaattttct gtacatgccc ggcggcggct cgctgaatcc tcccactcac
     4741 gataccgtcg ataaaaatat gcacgtagtc caccaggaag gttcgcaggt tttcaaatac
     4801 gctgtacgcc gcatggctga gctagccgta aatttgctcg aacgcaatgg tttcaagagc
     4861 gacgacctcg ccctggtcgt cccgcatcaa gccaacctcc gcatcattcg cgccatgcag
     4921 gagcgcctgg gcgtcgacga ttcaaaagta atggtgaaca tcgatcgcta cggcaacacc
     4981 accgcaggca ccatcccctt gggcctccgc gacgccgtag ccgaaggccg cctcaaaaag
     5041 ggcgacctcg tgctgctcgt tgccgtaggc gcgggctaca ccaccggcgg cgtcctgatg
     5101 cgctgggcct attaaaattt atttggctgt aatttctgtt tggcgtgttt ctaatgagcg
     5161 ccaggtcttc gtcggaagcg aatggcgctg aagcaaatgt cgtagctggt cagaaggaaa
     5221 gacgaaaact ctcaaacagt tgagaagaat gtctcagatt gagtattccc aggcgccata
     5281 tttgcggct
//
LOCUS       DATCBB010000025         5411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_260503, whole genome shotgun sequence.
ACCESSION   DATCBB010000025 DATCBB010000000
VERSION     DATCBB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260503"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VII37_00310"
     CDS             <1..1066
                     /locus_tag="VII37_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068185.1"
                     /translation="IGAHLHNLQDVDLTLPLGILTVVTGVSGSGKSTLVHDVLYKALA
                     AKRNGSSVKEFCERIEGDAQIREVVIVDQSPIGRTPRSNPATYLKAFDAIREVFADTP
                     DAKRRGYTAGHFSFNVPGGRCETCQGDGTVTVEMQFLADVELICEECRGTRFKSGVLE
                     VLYHGKSIHDVLQLTVREALVFFINVPKAISKLRVLNEIGLGYLRLGQSATTLSGGEA
                     QRLKLASHLSRTENQGILYILDEPTTGLHFDDIAKLLAAFRKLLESGASLLVIEHNLD
                     VIKSADWLVDLGPEGGDQGGKIIATGTPEQVARNAQSHTGRYLARVLNENGQAKGVQN
                     GASKIAGAVGMVNGSKVTSV"
     gene            1187..1816
                     /locus_tag="VII37_00315"
     CDS             1187..1816
                     /locus_tag="VII37_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068186.1"
                     /translation="MLATTMSQYADGQSGGQNSPDKTQGGSQGTSQGTVAGVRRERVA
                     NPLNDLLQEAQRDIEKKDFAAAIDPLQKFIAAQPDVAYGHFELGYVFTALQKADEARG
                     EYEKAIAIDPKMAEAYLNLGILFIERNPAVAVPNLRKAVELLPAQSRPRYLLGVAQEN
                     TKDYSAAAESFEGAYRLDPHNLEPILHLANLYIRLKRPADAEAKFRSIL"
     gene            1841..2524
                     /locus_tag="VII37_00320"
     CDS             1841..2524
                     /locus_tag="VII37_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068187.1"
                     /translation="MLGVAECLAAEKKPEAADAYRNYLRVQPADSAARARLVHLLVEQ
                     QQYDAALEELNRAEADQGTNVAANLDSLRMRADIQIAQKKWDDSIATLNKAIALAPGD
                     AQLHGGLGRIYLQKRDFPDALKELKTALQLDAKNVAYWKDLSTSYYLSGDCVATLATL
                     DQISKMETPGAGPWFIRALCYDKLNQPKPALEAYQQFLSLDQGKNSDQEWQAQERSKV
                     LRKMLEHKR"
     gene            2527..3081
                     /locus_tag="VII37_00325"
     CDS             2527..3081
                     /locus_tag="VII37_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068188.1"
                     /translation="MTVRRASCGLWVGAAMMCCVVSGGAAVATDKLGEMQARFDHEKN
                     PVHRAKLLEKLGDAEFDEARRAFKANDLSAVDTVLEKYRDNVRVAFEGLKKKSSDAEK
                     NSNGYRQLEIHVRRGIRETDEIILRVPEEYQPPLQLVHRDLDAMDKELIHMLFHHRDE
                     QPAAPKGADPATGPEHPASPAEDE"
     gene            3081..3602
                     /locus_tag="VII37_00330"
     CDS             3081..3602
                     /locus_tag="VII37_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068189.1"
                     /translation="MKVSRKIIGAVFCLMLFAGGSARAQEQKDYLSTLEADKIRDAET
                     TNDRVKLFLTFADDRLKKFQYELEHPSGTQHIDMLNSLLNGYGGCVDDAADLIQLGME
                     RQENIRAAIDLMASQSKSFLAVLEKLSAAGGPDLEAYKSNLDDAIEGTRDAMKEAGKA
                     TKKVAPPPIRRKN"
     gene            3627..4340
                     /locus_tag="VII37_00335"
     CDS             3627..4340
                     /locus_tag="VII37_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068190.1"
                     /translation="MKSYEVLQVPGGSALFQRVFTRLGCNGRPPRFRVEFYPYSSLVL
                     TIRRREEAMYVRFSDLLQRAPLPVLEGGAALLLSRVYRRKAPRDVVEPYLEYARSHKT
                     RNRISRMRSGRVRVRQSDPQGRHFDLSALFDELNTKYFDGALQRPHIGWSVRSWRRQF
                     GCYDPGPNQILLNRRMDRPGVPACAVEYVLFHEMLHVKHPTRRSGCSLVSHSREFREE
                     EKRFVGFERARRVLDRLAR"
     gene            complement(4329..4874)
                     /locus_tag="VII37_00340"
     CDS             complement(4329..4874)
                     /locus_tag="VII37_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068191.1"
                     /translation="MPIVTHQMQCPTARRGAIARWLLPAAAFLLLTAPASHAQDSQGE
                     SSSTAPKPLPPGPPDSSHPKSSNKKPKDTRTDSAPDQPTWDPLRAEKDLEVGQFYLKK
                     GDLDAAIDRFQDAAAAKPGFAIPYRYLGEAQEKKGDKREALKSYRRYLDLYPHAEDAK
                     KIQKKIDKLWSEVGQKDKSAG"
     gene            5037..>5411
                     /locus_tag="VII37_00345"
     CDS             5037..>5411
                     /locus_tag="VII37_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY5068192.1"
                     /translation="MEPREKAALLPEAPGVYLFRDAGGTVLYVGKAISLRSRVRSYFL
                     ESSWVNAKTGSLAREIADLETILVANAREALALENNLIKQYRPKFNVLLRDDKTYPYI
                     KLTASEKFPRVYFTRRVKKDGSL"
BASE COUNT         1204 a         1202 c         1767 g         1238 t
ORIGIN      
        1 aattggcgcg catttgcata atttgcagga tgtggatttg acgttgccgc tcggtatttt
       61 aacggtcgtt accggagtga gcgggtcggg aaaatccacg ctggtgcatg acgtgcttta
      121 taaagcgctg gcggcgaagc ggaatggttc gagcgtcaag gaattttgcg aacgcatcga
      181 gggggatgca cagattcggg aagtggtgat cgtggatcaa tcgccgatcg ggcgtacgcc
      241 gcgatccaat ccggcgactt atttgaaagc gtttgatgcg attcgggagg tttttgcgga
      301 tacgccggat gcgaaacggc gcggttatac ggcggggcat ttttcgttta acgtcccggg
      361 cgggcgttgc gagacatgcc agggcgacgg gaccgttact gtggagatgc aatttctcgc
      421 ggatgtggaa ttgatttgcg aggagtgccg cgggacgcgt ttcaagagtg gcgtgctgga
      481 ggtgctatat cacgggaaga gtatccatga cgtgttgcaa ctgacggttc gcgaggcgct
      541 ggtatttttt attaatgtgc cgaaggcaat ttccaagctg cgagtgctga atgaaattgg
      601 attgggttat ttgcggctgg ggcaatcggc gacgacgctt tccggtggag aagcgcagcg
      661 gttaaaactg gcgtcgcatt tatcccgaac cgagaatcag ggcatccttt atatactcga
      721 tgaaccaacg acgggactgc attttgacga tattgcgaaa ctgttggcgg cgttccggaa
      781 attgttggag tcgggggcgt cgctgctggt gattgagcac aatttagacg tgattaaatc
      841 ggcggactgg ctagttgatc tcgggccgga gggcggggat cagggcggga agattattgc
      901 taccgggacg ccggagcagg tggcgcgaaa tgcacaatcg cacacggggc gttatctagc
      961 gcgggtgttg aacgagaatg gacaggcaaa gggtgtgcag aacggagcat ccaagattgc
     1021 tggtgcggtg ggtatggtga acggatcgaa ggttacatcc gtttagtttg ttggcaacgc
     1081 gttaagcctt acaatttcat ctcaaatgcc aagctttgtg ttcgccagcc gggtcaacaa
     1141 aaacttactc aggctcgcat tttgtggttt ggaatttgct gcgattttgc tcgcgacgac
     1201 aatgtcgcag tatgctgacg ggcaaagcgg cggacagaat tctcccgaca aaactcaggg
     1261 tggatctcag ggcacatctc agggcaccgt tgcgggggtg cggcgggaga gggttgccaa
     1321 tcctctgaat gacctgctgc aggaagcgca gcgcgatatc gagaagaaag attttgcggc
     1381 ggcgattgat ccgctgcaga aatttattgc tgcgcagcct gatgttgcct atgggcattt
     1441 tgagcttggc tacgttttca ccgcactaca gaaggctgac gaggcgcggg gtgaatacga
     1501 gaaggccatc gcgattgatc caaagatggc cgaggcgtac ttaaatcttg ggattttatt
     1561 tattgaacgg aatccggcgg tggcagtccc gaatctacgt aaagcggtgg aattgttgcc
     1621 ggcgcaaagc cggccgcggt atttattggg tgtggcgcag gagaatacga aagattattc
     1681 cgcggcggcg gaatcgtttg agggcgccta ccggctggac ccacacaacc tggaaccgat
     1741 tctgcatctg gcgaatttgt atatccggct gaaacggcca gcggatgcgg aagcgaaatt
     1801 tcgcagcata ttataagttc agccgagtga tgcgcaggcg ttgctgggcg tcgccgaatg
     1861 cctggccgcg gagaagaagc ccgaggcagc ggacgcgtat cgcaactatc tgcgcgtgca
     1921 gcccgcggat tcagcggcgc gagcgcggct ggtacacctg ttggtggagc aacaacaata
     1981 tgatgcggcg ctcgaggagc tgaatcgagc cgaagccgat caaggtacaa atgtggcggc
     2041 gaatctggat tcgttgagga tgcgcgcgga tattcaaatc gcgcagaaaa aatgggatga
     2101 ctccatcgct acgctaaaca aggcgattgc gctggcaccg ggagatgcgc aattgcacgg
     2161 ggggttgggg cgcatttatc tgcagaaacg cgactttcca gatgcgttaa aagagctgaa
     2221 gacggctttg cagttggacg cgaagaacgt ggcgtattgg aaggatttga gcacgtctta
     2281 ctatctatcc ggggattgcg tcgccacgct ggcaacgctg gatcaaattt caaaaatgga
     2341 aacgcctggc gctggaccat ggtttattcg ggccctctgc tacgataagc tgaatcagcc
     2401 gaagcccgcg ctggaggctt accagcaatt tttaagtctg gatcagggga aaaactcgga
     2461 ccaggagtgg caagcgcagg agcgcagcaa agtgctgcga aaaatgctgg agcataaaag
     2521 gtaagaatga cggtgcggcg agcgagctgc gggttgtggg tgggtgcggc aatgatgtgt
     2581 tgcgtggtgt ccggtggcgc cgccgttgcc accgacaaac ttggcgagat gcaggcgcgt
     2641 tttgaccacg agaaaaatcc agtgcaccgc gccaagttgc ttgaaaaact gggagacgcg
     2701 gaatttgatg aggcgcgccg agcttttaaa gcgaatgatc tgagcgcggt ggacactgtg
     2761 cttgaaaaat atcgcgacaa tgtccgagtg gcgttcgagg gcttgaaaaa gaagagcagc
     2821 gacgcggaga aaaattcaaa cggttatcgc caactggaga ttcacgtgcg acgtggaatt
     2881 cgcgagacgg atgaaattat tttgagggtt ccagaagagt atcagccgcc gttgcagctt
     2941 gttcaccgcg atcttgatgc catggataag gaattgatcc acatgctttt tcatcatcgc
     3001 gatgaacagc cggcggcgcc gaaaggggcg gacccggcta caggtccgga gcatccggcg
     3061 agcccagctg aggacgagtg atgaaagttt caaggaaaat tattggtgcg gtgttttgtt
     3121 tgatgctgtt cgccggagga tccgcgcgtg cgcaggaaca aaaggattat ctctcgacgc
     3181 tggaggcgga caagattcgg gatgcggaaa ccaccaacga ccgcgtgaaa ctgtttttga
     3241 ctttcgcgga tgaccggctg aaaaagtttc aatatgagct ggagcatcca tcgggcacgc
     3301 agcatattga tatgctgaat tcgctgttaa acggatatgg cggatgtgtc gacgacgcgg
     3361 cggatttgat tcaattgggt atggagaggc aggaaaatat cagggccgcg atcgacctga
     3421 tggcctcgca gtccaaatca tttttggcgg tgctggagaa gttatcggct gcgggcgggc
     3481 cggatctgga agcgtataaa agtaatctcg atgatgcgat tgagggaaca cgggacgcca
     3541 tgaaggaggc tgggaaggct accaagaagg tggcgcctcc accgattcgt cgcaagaact
     3601 aaagaggaat cacagcgcga gatactatga agagttacga agtcttgcag gtgcccggag
     3661 gcagtgcgct gtttcagcga gtgtttacgc ggcttggatg taatgggcgg ccgccgcgtt
     3721 ttcgcgtgga attctaccct tattccagcc tggtgttgac gattcggcgt cgagaagagg
     3781 cgatgtacgt gcgattttca gatttgctgc aacgcgcgcc gttgccggta ttggaaggtg
     3841 gggctgcgtt gctattgtcg cgggtgtacc ggcgaaaggc gccgcgcgat gtggttgagc
     3901 cgtacctgga atatgcgcgt tcgcacaaga cgcgcaaccg tatcagccgg atgcgcagcg
     3961 ggcgagtgcg cgttcgacag agcgatccgc agggccggca cttcgatctt tccgcgttat
     4021 tcgatgagct gaacacaaaa tattttgacg gcgcgttgca gaggccgcac atcggctgga
     4081 gcgtgcgaag ctggcggcgg cagtttggct gttacgatcc agggccgaat cagattttgt
     4141 tgaacaggcg aatggatcgt ccaggagttc cggcttgcgc ggtggaatat gttttgtttc
     4201 acgagatgct gcacgtgaaa catcctacgc gacgatctgg ttgcagcctg gtatcgcact
     4261 cgcgggaatt tcgcgaagag gaaaagcgtt ttgtgggatt tgaacgcgcg cgtcgagtgt
     4321 tggaccgctt agcccgctga tttgtctttc tgtcctactt cactccagag tttgtcgatt
     4381 ttcttctgaa tcttttttgc gtcttcggcg tgcggataga gatcgagata gcggcgatag
     4441 gatttcaaag cttcgcgttt atcgcctttt ttttcctgag cttcgccgag atagcgatag
     4501 ggaatggcga agccgggctt ggcggccgcg gcgtcctgga agcggtcgat cgcggcgtcc
     4561 agatcgcctt tctttagata aaattgcccg acttcaagat ctttttccgc gcgcagcggg
     4621 tcccaggttg gttgatcggg cgcggagtcg gttcgagtat ctttaggttt tttgtttgag
     4681 gatttcgggt gggatgaatc tggcggtccg ggcggaagcg gtttgggagc ggtggacgag
     4741 gattcgccct gcgaatcctg ggcgtgggat gcgggcgcgg ttagaagaag gaaggcggct
     4801 gcgggcaaga gccatcgggc gattgcgccg cggcgcgctg tcgggcattg catctggtga
     4861 gtgactatag gcatctaatg aagaagagcg gatggcattc aatttatcct gcggtgatga
     4921 ctggtctcct cgtccttcca ctctctccga ttcactttag tatcatagta gttgtatgac
     4981 ccgtttgcat gccgcgttgc gcgatgcagg gaattgcggg acgctgacta aatagtatgg
     5041 aaccacgtga aaaagcggcg ctgttgcctg aagcgccggg agtgtatctg ttccgggatg
     5101 ccggcgggac tgtgctgtat gttggcaaag cgatttcgct acgcagccgg gtgcgctcat
     5161 attttctgga atcaagctgg gttaatgcca agaccgggtc gttggcgcgg gaaattgccg
     5221 atcttgagac cattctggtg gccaacgcgc gggaagcttt ggcgctggaa aacaacctta
     5281 tcaagcagta ccggccgaaa tttaatgtgc tgctgcgcga tgataagact tatccgtaca
     5341 taaaattaac ggcctcggaa aagttcccac gggtttattt tacgcggcga gtgaagaagg
     5401 acgggtcgct t
//
LOCUS       DATCBB010000026         1311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_325303, whole genome shotgun sequence.
ACCESSION   DATCBB010000026 DATCBB010000000
VERSION     DATCBB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325303"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VII37_00350"
     CDS             complement(<1..351)
                     /locus_tag="VII37_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068193.1"
                     /translation="MNSSTAFRQGIYKNELVRRITRWLDATPHPPLALEITPERVAAA
                     RFGRTGALDGYAVETLPQGAIVPSAVESNIGNPAQVKMAVSNACARLKAKDEDIALIL
                     PDPVIRVFVQHFDEF"
     gene            complement(344..>1311)
                     /locus_tag="VII37_00355"
     CDS             complement(344..>1311)
                     /locus_tag="VII37_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5068194.1"
                     /translation="RVPQDGRFRVKYKGRYIDLRVSIMPASHGEDAVLRVLDKETLSE
                     KFQSLSLDVVGFSADEMRRFRRYIREPYGMVLVTGPTGSGKTTTLYAAINEIKSDEDK
                     IITIEDPVEYQLKGITQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAI
                     QSALTGHLVFTTVHANNVTDVIGRFINMGVEPYNFVSALNCIMAQRLVRIICMNCRRP
                     KKFSPTELKEAGLDPGAWGGVTLMEGAGCLECSGTGYHGRTAICELLDLTDRIREMIV
                     DRRPTSEIKRIARDEGMITLRESGLAKIRDGITSVKEINKVTFVE"
BASE COUNT          272 a          370 c          359 g          310 t
ORIGIN      
        1 aaattcgtca aagtgctgca cgaatactct gatcaccgga tcgggaagga tgagcgcaat
       61 atcttcatcc ttggctttca acctggcgca ggcattactg actgccattt tgacctgcgc
      121 aggatttccg atgttggatt ccaccgctga tggcacaatc gcgccctgag gcagcgtctc
      181 aacggcatag ccgtccagcg ctcccgtcct gccaaagcgt gccgcagcca cacgttccgg
      241 agttatttcc agcgccagcg gcggatgagg agtggcatcc agccagcggg tgatgcggcg
      301 caccagctcg ttcttataaa ttccctggcg gaatgcagtc gagctattca acgaaggtca
      361 ccttgttgat ttctttcacg ctggtaatgc cgtcgcgaat tttggcgagg ccgctttcgc
      421 gcagcgtgat catgccttcg tcacgagcga tgcgtttgat ttccgaagta ggccggcggt
      481 ccacaatcat ttcacgaatg cgatcagtga gatcaagcag ttcgcagatg gccgtgcgcc
      541 cgtgataacc ggtgcccgag cattccaagc atcccgcgcc ttccatcagc gtcacgccgc
      601 cccatgcccc gggatcgaga cccgcttctt ttaattcggt gggtgaaaac ttcttcggcc
      661 gcctgcaatt catgcagata atgcgcacca gccgctgcgc catgatgcag ttgagagccg
      721 agacgaagtt gtaaggctcg acgcccatgt tgatgaaccg accgattacg tcggtgacgt
      781 tattggcgtg caccgtggtg aacactaagt gaccagtcaa ggcggattga atagcaattt
      841 gcgcggtttc ctgatcgcga atttcgccga ccatgatttt gtccgggtcg tggcgcaaaa
      901 tcgagcgcaa gccacgcgcg aacgtcaggc cttttttctc gttgaccggt atctgcgtaa
      961 ttcctttaag ttgatattcg acgggatctt caatggtaat gatcttgtct tcgtcggatt
     1021 taatttcgtt gatggccgcg tacagcgtgg tggtttttcc ggaaccggtc ggaccagtaa
     1081 ccagcaccat gccgtagggc tcgcgaatat acctgcggaa gcggcgcatc tcatcggcgg
     1141 aaaatccgac cacgtccagg ctcaagcttt gaaatttttc tgaaagcgtt tctttgtcca
     1201 ggacgcgaag cacggcgtct tctccgtggc tcgccggcat aatcgaaacg cgcaaatcga
     1261 tatagcgccc cttgtacttc acgcggaaac ggccgtcctg cgggacgcgg c
//
LOCUS       DATCBB010000027         1757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_325319, whole genome shotgun sequence.
ACCESSION   DATCBB010000027 DATCBB010000000
VERSION     DATCBB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325319"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..>1757
                     /locus_tag="VII37_00360"
     CDS             99..>1757
                     /locus_tag="VII37_00360"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY5068195.1"
                     /translation="MSKSKFTGKSRLGMLLCLTGLLSFNTATAVRAQDDGRPADDTAV
                     RPDQTIAQGPGDDPRYANSQADQDGGSPDAQDPPSRVARLSYLDGSVSFQAGGSGDWG
                     NAAKNRPVTIGDKIWVDKDARAELQAGQASIHLGAMTALSFLNLDQNITQMRLAEGSL
                     NFRVRELREGDTYEVDTPNVAFTVREAGAFRIDVNENGDGTIVTVIRGEGEVTAGGKS
                     HLVRTGELAEFNGTDSDIDYHTDRAPQPDDFDRWAADRDRREENSVSARYVSRDTVGY
                     SDLDDNGSWSQEPNYGPVWYPNSVAVGWAPYSNGYWGYVGPWGWTWIGYEPWGFAPYH
                     YGRWNYFGARWGWCPGPIGYSPVYGPAFVGFLGGGFGFGVGIGVGWFPLGFGEVYHPW
                     YGYRRGGAYIRNINIHNTYIRNVNNVNFNSANRNYAYAHNPAAVTATTRAGFANGQAV
                     NRGATQVSAASLRGAQVTNHLNVSPTRASYTGAANARGGRVATPPASVQNRAVIARTA
                     PAAAASHMAVRTVGGSSYNGGRSNVYAGNRSSGNSTAYNGRASAN"
BASE COUNT          374 a          465 c          545 g          373 t
ORIGIN      
        1 acttatttcc cgcagtgggg ttatacccat tggagactac ctgaggtaga ctccggccag
       61 ccggaagcaa gttccggcgt agaacagggg taataaatat gtcgaagagc aaattcaccg
      121 gcaagagcag acttggcatg ctgctgtgtc tgaccggtct tctttccttc aacacggcta
      181 cggcggtgcg agcacaggac gatggccgtc cagcggacga cacagccgtg cgtcccgacc
      241 agactattgc gcaaggccct ggcgacgatc cgcgctatgc caattctcag gcagaccaag
      301 acggcggttc accggatgcg caggatcctc cgtcccgcgt tgcccggttg agctaccttg
      361 acggctctgt ttcgtttcaa gcaggcggaa gcggtgattg gggcaatgca gccaagaacc
      421 ggccggttac catcggcgac aaaatctggg tagataagga tgcgcgcgcg gagttgcaag
      481 ccggccaagc ttcgattcat ctgggcgcga tgacggccct atcgtttttg aatctagacc
      541 aaaatattac gcaaatgcgc ttggcggaag gcagccttaa tttccgcgtt cgcgaacttc
      601 gcgaaggcga cacctacgaa gtagacacgc cgaacgttgc gtttacggtg cgcgaagctg
      661 gagcctttcg tatcgatgta aacgaaaatg gcgatggcac gatcgtgacg gtgattcgcg
      721 gagaaggtga agttaccgca ggcgggaaga gccacctcgt acgaaccggt gagctcgccg
      781 agttcaatgg tacggattcc gacatcgatt accacacgga ccgtgctccg cagcctgatg
      841 atttcgatcg ctgggctgca gatcgtgacc gccgggaaga aaattcagtt tctgcgcgct
      901 atgtttcgcg cgacacggtt ggttattccg atctcgacga taacggatcg tggtcgcagg
      961 agccgaacta cggaccggtt tggtatccca acagcgtcgc ggttggctgg gctccttaca
     1021 gtaatggata ttggggctac gttgggccat ggggctggac atggattggc tatgagcctt
     1081 ggggatttgc gccgtaccac tacggacgct ggaattactt cggcgcacgg tggggctggt
     1141 gcccgggacc gattggctac tctcccgtgt acgggcctgc tttcgttgga ttccttggcg
     1201 gaggctttgg cttcggtgta ggtattggag tgggttggtt tccactgggc tttggtgagg
     1261 tttatcaccc gtggtatggc taccgtcgag gtggcgcgta cattcgcaac ataaatattc
     1321 acaacacgta tatccgcaac gtgaacaacg tcaacttcaa cagcgcgaat agaaactacg
     1381 catacgcgca caatccggca gcggtaacgg cgactactcg agctggcttt gcgaacggtc
     1441 aggcagtgaa ccgcggtgcg acacaggtct cagcggcctc cttgcgtggc gcgcaagtca
     1501 ctaatcactt gaatgtgtct ccaacgagag ccagttatac gggggcggcc aatgcccgtg
     1561 gcggtcgcgt tgcaacacct ccggcttcag tgcagaatcg cgcggtgatt gcgcgaacgg
     1621 ctccagcagc agctgcgtcg cacatggcgg tgcgcactgt tggaggttct agttataacg
     1681 gcgggcgcag caacgtgtat gcgggcaatc gtagctcggg aaattccact gcatataacg
     1741 gcagggcgag cgcgaat
//
LOCUS       DATCBB010000028         1092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_260716, whole genome shotgun sequence.
ACCESSION   DATCBB010000028 DATCBB010000000
VERSION     DATCBB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260716"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..>1092
                     /locus_tag="VII37_00365"
     CDS             232..>1092
                     /locus_tag="VII37_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY5068196.1"
                     /translation="MKWTPGGESPDIEDRRDDSGGGGGGFQFGGMHIGIGGAIILLIL
                     SFVFKTNFFALLNGGGGGSMAPAPSATSDPNRPHPTSARNSARDQAEKPLVQFVSFVL
                     DDTQKTWTNVLPQQTNRPYRHAKLVLFRDAIQSGCGGAESATGPFYCPRDEKVYIDLG
                     FYDELKRRFGVPGEFAQAYVLAHEIGHHVQKILGIEAQEQQAVQQNPRLRNVMSVKLE
                     LQADCFAGIWAQNTQQRGLLEQGDIDSALSAAAAVGDDRLQKQATGHVSPDSFTHGTS
                     EQRMSWFRRGF"
BASE COUNT          240 a          258 c          380 g          214 t
ORIGIN      
        1 ctccacttcg cggtgcgact ccttcgcagg agcgaaggag aagaaaatgc gtcgcgccgc
       61 tccggtcgga atgacaggtt tgaggggatg gcgggagtac tgccaagatc aggtgagcga
      121 ccgcacacta aagagaaagc cgtgaagtac ggcggatgga acaaagtcgc gtgatgagcg
      181 gttgcggtgc ggcgtgctac tatttgcggg ctcggttgaa ggagaatcgc gatgaaatgg
      241 actccaggcg gggaaagtcc ggatattgaa gaccggcgcg atgacagcgg cggtgggggc
      301 ggcgggtttc aatttggcgg gatgcatatc gggattggcg gcgcgattat tttgctgatc
      361 ctgagttttg tgttcaagac gaattttttc gcgctgctga acggcggcgg aggcggctcc
      421 atggcgccgg ctccttcggc gacgagcgat ccgaacaggc cgcaccccac ttcggcacga
      481 aactcagcac gggatcaggc agaaaaaccg ctggtgcaat ttgtttcgtt cgtgctggac
      541 gacacgcaaa aaacgtggac gaatgttttg ccacaacaga cgaacaggcc gtaccggcac
      601 gccaagctgg tgttgtttcg cgacgctatt caatccgggt gcggcggcgc ggagtctgcg
      661 accgggccgt tttattgccc gcgggatgaa aaagtttata tcgacctggg attttacgac
      721 gaattgaaac ggcggttcgg ggtgcccggt gaatttgcgc aggcttatgt gctggcgcat
      781 gaaatcgggc atcacgtgca gaaaattctg ggaattgaag cgcaggagca gcaggcggtg
      841 cagcaaaatc cgcgattgag aaacgtcatg tcggtgaaac tggaactgca ggccgattgc
      901 tttgcgggca tctgggcgca gaatacgcag cagcgcggct tgctggaaca gggagatatt
      961 gattcggcgc tgagcgcggc cgcggcggtg ggcgacgatc ggttgcaaaa acaggcgacc
     1021 gggcatgtga gcccggattc gttcacccat ggcacgtcgg agcagaggat gtcgtggttt
     1081 cgcagaggat tc
//
LOCUS       DATCBB010000029         1747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_422871, whole genome shotgun sequence.
ACCESSION   DATCBB010000029 DATCBB010000000
VERSION     DATCBB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422871"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VII37_00370"
     CDS             complement(<1..247)
                     /locus_tag="VII37_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068197.1"
                     /translation="MKVIRYGKYVGEPADAVDLDELVKRMGDFFLQSGFESQYYGVSE
                     MDNQRSMEALKDAILRALQEGDLLPEDAMSEELRKMLQ"
     gene            complement(292..>1747)
                     /locus_tag="VII37_00375"
     CDS             complement(292..>1747)
                     /locus_tag="VII37_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068198.1"
                     /translation="ELRTSPFSEVRLRSRHVKDELRDNLVARVRQGGPIFPGIVGYDD
                     TVVPQIVNAILSRHNFILLGLRGQAKSRILRGLINLLDAEIPVVVGCEINDDPLKPIC
                     RACREKIAAEGDNTPIAWMPRDSRYVEKLATPDVTMADIIGDVDPIRAARGGRDLSDE
                     LTIHYGLLPRANRGIFGINELPDLAGKIQVGLFNIMQEGDIQIKGYPLRLPLDVLLVF
                     TANPEDYTARGKIITPLKDRIGAEIRTHYPASVKEGMVITAQEAWVRRSAGRAIEVPA
                     YLRETVEEIAFQAREDKRVDRRSGVSQRLPITTLESVVSSAEQRAARTGENSSYASVA
                     DIYAALPAITGKMELEYEGELKGADTIARELIRTAVGRVFTKYFGEVNFQPVIQWFEL
                     GGELKLPELAPTDERYRQLSTIQGLSEHVGRIGATDTKNHAAVAAASEMILEGLWAHR
                     RIGRSEERGYYAEKQKPQEPREPQSSRGPRRQFN"
BASE COUNT          346 a          510 c          494 g          397 t
ORIGIN      
        1 tctgcagcat cttgcgcaac tcttcgctca tggcgtcttc gggcagcagg tcgccttcct
       61 gcagagcacg caagatagcg tccttaagcg cttccatgga gcgctggttg tccatttccg
      121 acacgccata atattgggac tcaaagccgc tctgcaggaa gaagtcgccc atgcgcttca
      181 cgagctcgtc aaggtcgacg gcgtccgccg gctcaccaac atattttccg taacgaatta
      241 ctttcataat attccccggg cccgactgac tttattcgtt gcgcgcttct gttaattgaa
      301 ctggcggcgc ggcccgcgcg atgactgagg ttcacgcggc tcctgcggct tttgtttctc
      361 cgcgtaataa ccgcgctctt cactgcggcc aatgcggcga tgggcccaca gcccttctag
      421 gatcatttct gaagcagcag ctaccgcggc gtgattctta gtatccgtgg cgccgatgcg
      481 gccaacatgc tcgctcaggc cctgaattgt cgataattgg cggtagcgtt catcggtggg
      541 ggccagctca ggaagtttca actcaccgcc cagttcgaac cactgaatca ccggctgaaa
      601 atttacctcg ccaaaatact tggtaaaaac gcggccgaca gcggtgcgaa tcaattcacg
      661 cgcgatggtg tcggcgcctt tcaattcgcc ttcgtattcc aactccattt ttccggtgat
      721 ggcgggtagt gcggcataaa tgtctgcgac gctcgcgtag gaactatttt ctccggtgcg
      781 ggcggcacgc tgctccgcgc tgctgacgac gctttcgagc gtggtgatcg gcaagcgctg
      841 gctcactccc gagcgccgat cgacgcgctt gtcttcgcga gcctggaagg cgatttcttc
      901 cacggtctcg cgaaggtaag cgggcacttc aatggcacgc ccggctgagc ggcgcaccca
      961 cgcttcttgc gccgtgatga ccatgccttc cttcacgctg gcagggtagt gggtacgaat
     1021 ttccgcgccg atgcggtctt tcaatggagt aataattttt ccgcgcgcgg tgtaatcctc
     1081 tggattggcg gtaaagacga ggagcacgtc aagcggcaag cgcagcgggt accctttaat
     1141 ctggatatcc ccctcctgca tgatgttgaa aagcccgacc tgaatttttc ccgccaaatc
     1201 gggcagttcg ttaatgccaa atattccgcg attggcgcgg ggaagcaaac cgtagtgaat
     1261 ggtcaactca tcggatagat cccggcctcc ccgagccgcc cgaatgggat caacgtcacc
     1321 gatgatgtcg gccatggtga cgtcaggggt ggctagtttt tctacatagc ggctatcgcg
     1381 cggcatccag gcgatgggcg tgttatcgcc ttcagcggcg attttttccc ggcacgcgcg
     1441 gcagatgggt ttcagcggat cgtcgtttat ttcgcaacca accaccacag gaatttccgc
     1501 gtcgagcaaa ttaatcaggc cgcgcaaaat gcggcttttg gcctggccgc gcagtcccaa
     1561 caaaatgaaa ttgtggcgcg acaagatggc gttgacgatc tgagggacga ccgtatcgtc
     1621 gtatcccacg ataccgggaa aaatcgggcc gccctggcga accctggcga ccaggttatc
     1681 gcgcagttcg tccttgacgt gccgtgaccg gagacgtact tcagagaaag gactggtgcg
     1741 cagctcg
//
LOCUS       DATCBB010000030         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_33679, whole genome shotgun sequence.
ACCESSION   DATCBB010000030 DATCBB010000000
VERSION     DATCBB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33679"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VII37_00380"
     CDS             <1..637
                     /locus_tag="VII37_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5068199.1"
                     /translation="GFTKDQDKLLTEIKRTELDTEPAVAPPSAPMNEGPVINGHSAIG
                     DSPNVAPTVNIIKGQTTKCLDDAVYSAAQVLKDRGRERRKMIFLISDGANGIRANTHD
                     YKSTVKELLRYGVSVYSVAVSSAYFERRFSRLVDYAHDSGGDVYFAAKRSTMEELYSR
                     VTEEARNQYTLAYSPAGTNRALDYHTIEIRVKREGLTVDAREGYYAGAAPH"
     gene            complement(1075..>2249)
                     /locus_tag="VII37_00385"
     CDS             complement(1075..>2249)
                     /locus_tag="VII37_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5068200.1"
                     /translation="KGLEFPHVFLLRLNNKAFPASERSRVFEFPAELMKEGAPAEQFH
                     IQEERRLFYVALTRARERLTITTLTDKKGKVPTFVEDIVMDPLVKRRDVVQMAPRVAP
                     RVASDEVPSGAPVAASLFPAPIDPPRIFSRIASWAETFHPPCPEPLKLSPSALDNYRR
                     CPQQYLFGRLWALEEGPRGTLTFGRVVHDTIRRTLAELRKGNMLPFEEVQRIFETEWK
                     SAGFEDDYQENEYKKDGLEQLKVFHAAMLESLPEILEQEKTFELPMENNVIIAGRIDQ
                     VNALGRNDVEIVDYKTGKPKRELDARRDLQLSIYALAAVEIFEWNPVRLVFHYLQNNQ
                     IQATTRDTKQLQEAQKIVQEVAADIRAGQFPAKPGYQCRNCAYRAICPSHEEALCS"
BASE COUNT          510 a          748 c          499 g          492 t
ORIGIN      
        1 aggcttcacc aaggatcagg acaaattact caccgaaata aaacgcaccg agctggatac
       61 cgagcccgcg gtagcgccgc catccgcgcc catgaatgaa ggtccagtaa tcaacggcca
      121 ctccgctatc ggagattccc ccaacgtcgc ccccaccgta aacatcatca aaggccagac
      181 caccaagtgc ctggatgatg cggtctacag tgccgcgcaa gtcctaaaag atcgcggccg
      241 cgaacgccgc aagatgattt ttctgatttc cgatggcgcc aacggaataa gagccaacac
      301 ccacgactac aaatccaccg taaaagaatt gctgcgttac ggcgtctccg tttatagcgt
      361 cgccgtcagc agcgcctatt tcgaacgccg cttcagccgc ctggtcgatt acgcccatga
      421 ctccggaggc gatgtctact ttgctgccaa gcgcagcacc atggaagaac tctattcccg
      481 cgtcaccgaa gaagcccgca atcaatacac cctggcctat tcccccgccg gaacgaatcg
      541 cgctctcgac tatcacacca tcgaaatccg cgtgaagcgc gaaggcctca ccgtagacgc
      601 ccgcgaaggc tactacgccg gcgccgctcc ccattaatta ccgccgacgt cccattaatt
      661 gatcgccaaa atgaaatcca tgcaccgggt ggcctagcct ttgccagttt tgcaaaggga
      721 ggcatttttc tccgattgct ttcctcagcc acgcattgcc cagcgtcctc ggcccttcat
      781 ttcgtgaccg cttcataaaa attttatttt ttgctgctag tgtgcgcgac ctatttgtga
      841 gacaactata tcgagccgag cgtttttttc cggccattga aaattgcgga ggtgtttgtg
      901 aagaagaagc cagcaaagaa gaccgccaca aaaaaaggta agtagcacct caatcagatt
      961 gaaaaaaagg gcgccgcggt tttttttcgc ggcgcccttt cttattttcc cctactcgtt
     1021 catctatttc taaatttcga atcgcggtct aacagctccc tctaccggct tcgtctacga
     1081 gcacaacgct tcttcatggc tcgggcagat tgcccggtaa gcgcaattgc ggcactggta
     1141 ccccggctta gccggaaact gccccgcgcg aatatccgcc gccacctcct gcacaatctt
     1201 ttgcgcctcc tgcagttgct tggtatcccg cgtcgtcgct tgaatctgat tgttctgcag
     1261 gtaatgaaac accagccgca ccgggttcca ttcaaaaatc tccaccgccg ccagcgcata
     1321 aatgctcaac tgcaaatccc gccgcgcatc cagctctctc ttcggcttcc cggttttgta
     1381 atccacgatt tccacgtcgt tgcgccccaa cgcattcacc tggtcaatac gcccggcgat
     1441 aatcacatta ttttccatcg gcaattcgaa cgttttttcc tgctccagaa tttccggcag
     1501 gctctccagc atcgccgcgt gaaacacctt cagctgctcc agcccatcct tcttgtactc
     1561 gttctcttgg taatcatcct caaatcccgc cgatttccat tccgtctcaa atatccgttg
     1621 cacttcctcg aacggcaaca tgtttccctt acgcaattcc gccagcgttc ttcgaatcgt
     1681 gtcgtgcacc acacgcccaa atgtcaacgt cccgcgcggc ccttcttcca gcgcccacag
     1741 ccgcccaaac aaatattgtt gcgggcatcg gcgataatta tccagcgccg aagggctcag
     1801 cttcagcggc tccgggcaag ggggatgaaa tgtctccgcc cacgaagcaa tgcgcgaaaa
     1861 aatccgcggt ggatcaatcg gcgccggaaa cagcgaagcc gccaccggcg cgccgctcgg
     1921 aacctcatca ctcgcgaccc gcggagccac tcgcggcgcc atctgcacca cgtcccgccg
     1981 cttcaccagc ggatccatca caatatcttc gacaaacgtc ggcactttgc cttttttgtc
     2041 cgtcagcgtg gtaatcgtca gccgctcccg cgcccgcgtc agcgccacgt aaaacagccg
     2101 ccgctcttcc tgaatatgaa attgttccgc aggcgcccct tctttcatca gctcggcagg
     2161 gaattcaaac acgcgggacc gctccgaagc cggaaacgcc ttgttattca accgcagcaa
     2221 aaacacatga ggaaactcca gccccttcg
//
LOCUS       DATCBB010000031         4168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_98588, whole genome shotgun sequence.
ACCESSION   DATCBB010000031 DATCBB010000000
VERSION     DATCBB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98588"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      45..258
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            319..2622
                     /locus_tag="VII37_00390"
     CDS             319..2622
                     /locus_tag="VII37_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5068201.1"
                     /translation="MKLRGLLRFSILLLVLLSCKTSSLAQNKSGNAAISGTIVDATGG
                     AISGVMVTATFEENRKVAASVTSKADGSYTLEVSAGRYRVRFMRAPFSPVEELVDVGA
                     GESRVLESRLTLERLSSSVVVTAQAEPALLEQATAPVSIISRAEIDARQAVSVTDALQ
                     FAPGIAIGRTGAEGGSASVFLNGGNSNFTKVLVDGAPINPPGGAVDFSSLTLDNIDKV
                     EIVRGAESAIYGTDAISGVIQLFTHRGETRVPEVDVFAEGGSFSTARGGAEVGGLVGN
                     FDYSAAGSYLETDGQGPHDNFINRTVSGNFGYRFSETNQLRLTLRNNTSNAGIPGQTV
                     FEPPSLHQRYDQRIFSANARWDFSTGTHWHHEISGSEMYTRQHNYNPIQSFFAADPNV
                     FCPQANPASVPTAEFCDSTNDSRIQYNRADVSAQSSYVRSNFGATAGYEYEVENAFLS
                     SLDVNHARRNNQGGFLDFRYRPIRRVSLNFGGRAEANANFGTRVVPRIGGSVVARYGT
                     GLVGDTLFRAFYGQGIKEPRFDQTLGDNFGDFGNPALKPESSKNWSGGIEQKLLNDRV
                     RVTADYFYYSFYNIVSFAFCLPDAVNPAGNSCGVIIPGAPPTGFGFFFNTDRALARGI
                     SVTGESRFTRWLNLAGNYTYDDTRVLQAPNAFDPSELPGNHLIRRPVNSGSLTLNAQW
                     RRVGLTIGGYFTGVRTDSDFLGLGLSRNPGYARFDIAGHFDLGHGVFLYARAQNIFDK
                     QYQDVLGYAALGRDARIGVRYTFKGRD"
     gene            2622..3332
                     /locus_tag="VII37_00395"
     CDS             2622..3332
                     /locus_tag="VII37_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068202.1"
                     /translation="MGQREPVLFCWSGGKDSAMALHALIQDPWVRVVALLTTVTEGYE
                     RVSMHGVRRELLRRQVESIGLPLHEVLIPPECVNLIYEARMEAALRKHFENGIRKVAF
                     GDIFLEDLRVYREKNLARIGMTALFPIWKRDSRELIGSFHAHGFRSIAVCVNPKLLDR
                     SFAGRELDHSFFADLPPQVDCCGENGEFHTFAFDGPIFKDAVRFQVGERVDRDSFVFC
                     DLLPEKECQQYESASNKA"
     gene            3307..3858
                     /locus_tag="VII37_00400"
     CDS             3307..3858
                     /locus_tag="VII37_00400"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEY5068203.1"
                     /translation="MNPQATKREETIFRTMLIVAMILFAAIIRIVPHPWNLAPVGAMA
                     LFSGAVVRNRVMAFVFPLLGMLAGDFFVGFHILMPVVYVSFVVSTAIGFWLRERRGVA
                     RLGGAVLLGAIQFFLITNLGVWMFLNIYPKTFAGLMACYAAGVPFFWNTLAGDACYSA
                     LLFGGLFLAERTYPALRPNVSAV"
BASE COUNT          817 a         1000 c         1323 g         1028 t
ORIGIN      
        1 agttgccgcg gtaaaacatt taacccatag cgggcctagc cccccacaat caccgcggat
       61 ttcgcgctcg ggaagacggt tagttgatgg aattttttca ttagctaaat aagccgtcgc
      121 cgccgcgcga ctgtaaggcg ggaattgggt ttccggccac tgcagcgagt tgctcactca
      181 ggtgggcgcc gttgcgggaa ggcctggagc cggcagtgcc gccaagccag gagaccggcg
      241 aaatccgcag cctgcgagag aggtttatcg cgcaggacaa ccttcagagc tcccgcgcga
      301 gagggagtgg aggatgaaat gaaactacgt ggacttcttc ggttttctat tctgctattg
      361 gttcttctga gctgcaaaac ttcctcactg gcacaaaata aatcagggaa tgctgcgatt
      421 tccggaacga ttgtggacgc tactggtggt gcaattagtg gcgtgatggt gactgcgacg
      481 ttcgaggaaa accgcaaggt tgcggcgtcg gtgacttcga aggcggacgg tagttacact
      541 ttggaagttt cagcgggacg gtatcgcgtg cggttcatgc gagcgccgtt tagtccagtg
      601 gaagagcttg tcgatgtggg cgcgggagaa tcgcgcgtct tagagtcgcg tcttacgctg
      661 gagcgcctat cttccagcgt ggtggtcacg gcgcaagcgg agccagcgct gctggagcaa
      721 gctacagcgc cggtctcgat tatttcgcgg gcggaaattg acgcgcgcca ggcggtctct
      781 gtgactgatg cgctgcaatt cgcgcccggc attgcaatcg ggcgcactgg cgcagaggga
      841 ggtagcgctt cggtttttct gaatggtggt aattccaact ttacaaaagt gctggttgat
      901 ggcgcaccga tcaatccgcc gggcggtgct gttgatttct ccagcctgac gctggacaac
      961 atcgataaag tcgaaatcgt tcgcggcgcg gaaagcgcga tttacgggac tgatgcaatt
     1021 tccggcgtga ttcagctgtt tacccatcgt ggggagacgc gcgttccgga agtagatgtt
     1081 tttgcagaag gcgggagttt ttctactgca cgcgggggag ctgaggttgg cgggttagtg
     1141 ggtaacttcg attactcggc ggccggttcc tatttggaga ccgacggaca agggccgcac
     1201 gataacttca tcaatcgcac ggtctcgggc aatttcggtt atcgcttcag cgagaccaat
     1261 cagttgcgct tgaccttgcg caataacacc agcaatgcgg gcattcccgg gcaaacagtc
     1321 tttgaaccgc cgagcttgca ccagcgttac gaccagcgca tttttagcgc gaacgcgcgc
     1381 tgggattttt ctaccggcac gcactggcat cacgaaatca gcggtagtga aatgtacacg
     1441 cgacagcaca actacaaccc gattcagagt ttctttgccg ccgatcctaa tgtgttctgc
     1501 ccgcaagcga atcctgcgtc ggtgcccacg gcggaattct gcgactccac caacgacagc
     1561 cgcattcaat acaaccgcgc tgacgtgagc gcgcagagca gctatgtacg ttccaatttt
     1621 ggggcgacgg cgggttacga atatgaggtg gagaacgctt ttctgtcgag tctggacgtt
     1681 aaccatgcgc ggcggaataa tcagggcggg tttttggatt ttcgttaccg gccgattcgg
     1741 cgcgtgtcgt tgaatttcgg tgggcgtgcg gaggcgaacg ccaactttgg cacgcgggtg
     1801 gttccgcgca ttggtggttc ggtggtggcg cgttacggga cggggttggt tggggacact
     1861 ttgttccgtg cgttttatgg gcaagggatc aaagaaccgc gctttgacca gacattgggg
     1921 gacaatttcg gcgattttgg caatccggcg ttgaaaccgg aatcgagcaa gaactggagc
     1981 gggggcatcg aacaaaagct gctgaacgat cgggtgcggg tgacggcgga ttatttttat
     2041 tacagcttct acaacattgt gagctttgcg ttttgtctgc cggatgcggt gaatccggct
     2101 gggaatagtt gcggagtgat tattccgggt gcgcccccga cgggatttgg atttttcttt
     2161 aatacggacc gggcgctggc gcggggaatc agcgtaaccg gagaatccag attcactcgc
     2221 tggctcaacc tggcgggcaa ttacacctat gacgatacgc gggtgctgca ggcaccgaac
     2281 gcattcgatc cttcggaact gccgggaaat catcttatac ggcggccggt gaattctgga
     2341 tcgcttactc tgaacgcgca gtggcgtcgc gttggtttga cgattggcgg gtacttcacg
     2401 ggagtgcgca cggattcgga ttttcttggg ctcgggctct cgagaaatcc ggggtacgcg
     2461 cggtttgata ttgccgggca cttcgacctt ggtcacgggg ttttcttgta tgcccgggcg
     2521 caaaatatct tcgataagca atatcaagat gtgctgggct atgcggcgct ggggcgcgat
     2581 gcgcgcatcg gtgtgcgtta tacgttcaag ggtagagact agtgggccag cgtgagccgg
     2641 ttcttttttg ctggagcggc gggaaagaca gcgcgatggc gctgcacgcg ctgatccagg
     2701 atccgtgggt gcgggttgtg gcgctgctga cgacggttac cgaggggtat gagcgcgtca
     2761 gcatgcatgg cgtacggcgg gaactgttgc ggcggcaggt ggagagtatt gggttgccgt
     2821 tgcacgaagt gttgattccg ccagaatgcg tgaatttgat ttatgaagcg cgcatggaag
     2881 cggcgctgcg gaaacatttt gaaaatggga ttcgcaaggt cgcgttcggc gatatttttc
     2941 tggaagattt acgcgtttac cgggagaaga acctcgcgcg tatcggcatg acggcgctgt
     3001 ttccgatctg gaagcgggac agccgcgagc taatcggttc atttcatgcg catgggtttc
     3061 gttcgatcgc ggtgtgcgtg aatcccaaat tattggatcg cagtttcgcg gggcgtgagc
     3121 ttgatcattc gtttttcgcg gatttgccgc cacaagtgga ttgttgtggc gagaatggcg
     3181 aatttcatac cttcgctttt gatgggccga tctttaaaga tgcggtccgc tttcaagtag
     3241 gcgagcgcgt agatcgggat tcgtttgttt tttgtgatct gttgccagaa aaggagtgcc
     3301 agcagtatga atccgcaagc aacaaagcgt gaagaaacta tattccgcac gatgttgatc
     3361 gtggcaatga ttctcttcgc agcgataatt cgcatcgttc cccatccctg gaatctcgct
     3421 ccagtcgggg ccatggcgct tttctcggga gcagtggtgc ggaaccgcgt gatggcgttt
     3481 gtgtttccgc tgctggggat gctggcaggc gacttttttg ttggctttca cattctaatg
     3541 ccggtggttt acgtcagttt tgtggtcagc acagctatcg gtttctggct gcgagagcgg
     3601 cgcggcgttg cgcgtctcgg cggagctgtg ttgctaggcg cgatacagtt ttttctgatc
     3661 acgaatctgg gcgtgtggat gtttttaaac atctatccca agacttttgc gggcttgatg
     3721 gcttgttatg cggccggagt gccatttttt tggaatacgc ttgccggaga tgcgtgctac
     3781 tctgcgctgc tgttcggcgg attgttcctg gcagagcgca cctacccggc gttgaggccc
     3841 aacgtttccg cagtttagca ggttgccgat tgggcaatgg gtcagtttcg gataatttgc
     3901 gttaatcggg cattttgcag aacaaccttc cctttcgtga tcggacggaa ggctagctgt
     3961 tttcgagaaa ctcaaaagtc aatacgactt tgactacggt cacgccgctg tcctgagccc
     4021 gcgtagtagc atcatcgtta gtatttcctt gcaacgcggt ggcgctggat tggcggaggc
     4081 tcccatgaag aaagaatttg cgctcgaccc gggcggtccg aagcgcctca ctgtaacgta
     4141 ccccggaaac cttgctaatg ccgaggtc
//
LOCUS       DATCBB010000032         6753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_1280, whole genome shotgun sequence.
ACCESSION   DATCBB010000032 DATCBB010000000
VERSION     DATCBB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VII37_00405"
     CDS             complement(<1..229)
                     /locus_tag="VII37_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY5068204.1"
                     /translation="MPDETEIKLKIRDLKAFQRILKRLGAKPVGSTTGRVHEENLIFD
                     TPDGGLAKHGQLLRLRTETAAPPSKKSTKTKK"
     gene            344..1087
                     /gene="map"
                     /locus_tag="VII37_00410"
     CDS             344..1087
                     /gene="map"
                     /locus_tag="VII37_00410"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY5068205.1"
                     /translation="MSIKTAEELAGMRAAGLVVRRTLEAMKRAVRPGVTTAEVDDAGA
                     KVIREHGARPAPALVYGFPGVNLISVNNEVVHGIPGARKIREGDLVKLDVTIEKDGFM
                     ADAAVTIGVGKISEQKSRLMACAERAFHHAMKFARAGVRVAEIGGAIEREVRRSGFSV
                     IRELGGHGIGRTIHEPPSVPNYLERNSKDILTKGLVITVEPIIASGSGKVFVAADGWT
                     VETNDGGMAAHYEHTLVITKQQPILLTAA"
     gene            1260..3506
                     /locus_tag="VII37_00415"
     CDS             1260..3506
                     /locus_tag="VII37_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5068206.1"
                     /translation="MNNDDSKTTATTLPVTETNGEGVPQQNAGTAAKAGGGVNGVSPP
                     IARREPEAMVLHDDRRVDHYAWLRNKESAEVIDYLKAENAYTDAVLLPTEALQEKLYQ
                     EMLGRILQTDLSVPYRLRGYSYFARTEEGKQYPVRCRRRAADGAPEELLLDLNALAEG
                     HSFLGLGAFEVSDNNDLLAYATDTTGFRQYALQVKKLASGETLGERFERVTSVAWAAD
                     NRTLFFTEEDETTKRSHRLYRHVLGSGEAPILLYEEKDERFRIGVERTRSGSFLLLTS
                     ASHTTSEVRFLSAKHAGGNFSLIEPREDNHEYYVEHHPGDEDSSDAGVFYIRTNSGGR
                     TFRLMEVSTKEPSRELWREVIPNRPEVMLAGADAFENHLVLFEREGGLPYLRIIDLEN
                     IGDESEDRYGDLLNDSHRIEFTEPAYNASLGTNPEFVTNFVRFQYESFVTPRSVFDYN
                     VPTRERLLLKQQPVLGGYDAQRYVTERLHATAADGTQIPISVVYRRDTFAREPKWPTD
                     RSTDAPLLLYGYGSYGISVPVTFSSNRLSLLDRGVVFAIAHIRGGGELGKPWHDAGRM
                     RQKMNTFTDFIAAAEFLIAQRYTSPEKLVIEGGSAGGLLIGAVVNLRPYLFHAAISHV
                     PFVDVLNTMLDASLPLTVGEYEEWGNPQVAEDYWVMKSYCPYTNVTRCAYPAMLVKTG
                     LNDSQVMYWEPAKYVAKLRAMKTDTNPLLFKVNMGAGHGGASGRYDYLREVALDYAFV
                     LTQSGIRE"
     gene            3592..6276
                     /gene="mutS"
                     /locus_tag="VII37_00420"
     CDS             3592..6276
                     /gene="mutS"
                     /locus_tag="VII37_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY5068207.1"
                     /translation="MWLTSVIEATTPLMQQYQGIKSRYPHALLLFRLGDFYELFYEDA
                     IIAARELQITLTSRNREKGQPIPMCGVPYHAADGYIARLIRAGFKIAICDQMELPGPG
                     RKIVRREVVRVITPGTATDGAVLEARENNFLAAVAKDAGGTLGMAYVDLSTGEFQATE
                     FSGPGAAEALRDELQLLRPRETLLPRPQQLFETAKTSLLDGTGGVESRLDEWIFQHDY
                     AERILREQFGVAELTGFGLDGHQQSMAAAGAIIHYLRENAARGDDAHSVEALRHLDGV
                     RYYEQHNALVLDPVSVRNLELINPIFTEESGRAGPTTLIAALDVTVTGMGARLLRSWM
                     MRPLVDPVAIEARLNAVAHLVQQTMVRGEIRKELKGILDLERITSRITLGLATPRELV
                     ALRKSLARIPLLKNFLTPPSAGGSELLRRIFTEIDELSDVRERLERGISDEPPALATT
                     PGMIRSGFDGELDELRNLSQHSKEIIAAMEERERKRTGIASLKIRFNSIFGYYIEISK
                     SNLHLAPADYERKQTLVNAERFTSAELKEYERQILAADERIAEIENRLFVELRSGVAL
                     HAARLRVTASAIAQLDVLTSFAKLAAERGYCRPEFNATGELLIVDGRHPVIEELLRQR
                     GERFVPNDLYLESQRQQLLLITGPNMGGKSTYLRQAALIVLMAQMGCFVPARQAKLPV
                     TDRIFTRIGASDNLARGRSTFLVEMSEVATILHHATPSSLVLLDEVGRGTSTFDGLSI
                     AWAVVEHLQKHTGARTLFATHYHELTELAELLSAVKNVHVSVKETPNEIVFLRRVEPG
                     SADKSYGIEVARLAGLPRSVIERAREVLLKHEQNEHELSETLSPAVNGNGSHNGSQAV
                     LFTALDREVIEKLRGADLDQLKPLDALNLLAELKKQIS"
     gene            6273..>6753
                     /locus_tag="VII37_00425"
     CDS             6273..>6753
                     /locus_tag="VII37_00425"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY5068208.1"
                     /translation="MSTGATKGGMLVLGLMSGTSADGIDIALASVSGAPPRVEARLLN
                     HTAVKFPARVRAEILRVAEQRAITAGELSQLNFRLGGLFADAAKTACKRFRVALSSVD
                     LIGSHGQTIFHQGVPANYLGAPTASTLQIGEAAVIAAGTGVTTVADFRPADMAVGGQG
                     "
BASE COUNT         1460 a         1571 c         2203 g         1519 t
ORIGIN      
        1 attttttggt cttggtggat tttttggaag ggggtgcagc tgtttcggtg cggagcctta
       61 ggagttggcc atgtttggcg agacctccgt cgggcgtatc gaaaattaga ttttcttcat
      121 gaactcgccc agtggtagaa cccacgggct tggctcccaa gcgtttcagg atgcgttgga
      181 aggctttgag atcgcgaatt ttaagtttga tttcggtttc gtccggcatg gttcttccgt
      241 cataagcgaa aatggcgagt aagatggtgc cagttaagca gtaagaaatc gagttctatc
      301 taggcgtggg ttcgcacagc ggcgaacggc cgggaggtcg gcaatgtcga tcaaaaccgc
      361 agaagaattg gcagggatgc gtgctgctgg cttggtggtt cgccgcactt tggaagctat
      421 gaaacgcgcg gtgcggcccg gagttactac cgctgaggtg gatgacgctg gagcgaaagt
      481 gatccgcgag cacggagctc gaccggcgcc cgcgttggtt tatggctttc ctggcgtgaa
      541 tctaatcagt gtgaataatg aagtggtgca tgggattcct ggtgcgagga aaattcgcga
      601 gggcgatctg gtgaaattgg acgtcaccat agaaaaagac gggtttatgg cggacgcggc
      661 cgtaactatc ggcgtgggga aaatttctga gcagaaatct cgcttgatgg cgtgtgcgga
      721 gcgcgcattt caccatgcca tgaagtttgc gcgggctggc gtgcgtgtgg cggagatcgg
      781 aggtgcgatc gagcgagaag tgcggcgaag cgggttctcg gtgataaggg aactcggagg
      841 acacgggatc gggcgcacga ttcatgaacc gcccagcgtt cccaactatc tggagcgaaa
      901 ttccaaggac atattgacta aaggtttggt cattacggtg gaaccgatca ttgcgagtgg
      961 atcgggaaag gtatttgtgg ccgccgacgg ctggacggta gaaaccaacg acggcggaat
     1021 ggccgcgcat tacgaacata ctttggtgat tacgaagcag caaccaattc tgctgacagc
     1081 agcttgagtt tagatgatcg gaaatctcga gaattagttt gcgggatggc ggctttctac
     1141 gcgtgcgaag ttccgctagt ataacgagcg aagccaagtt ttatccgaac cacacggtat
     1201 cgacgcgtcg cgctcgctct tctggcgatg cgcctgcgca aaattaccaa ccggggcaaa
     1261 tgaacaacga cgactcaaaa acgactgcca ctactcttcc agttaccgag acgaacggcg
     1321 aaggggtacc tcagcaaaac gcggggaccg ctgcaaaagc cggcggaggc gtcaatgggg
     1381 tgagccctcc cattgcgcga cgagaaccgg aagcaatggt cctgcacgat gaccggcgcg
     1441 tggatcatta tgcgtggctg cgaaataagg agagtgcgga ggtcattgat tatttgaagg
     1501 cggagaacgc gtataccgac gctgttctgc tgccaacgga ggcgctgcaa gaaaaactgt
     1561 atcaggaaat gctggggcgg attttgcaga cggacttgtc cgtaccgtac cggctgcgcg
     1621 gctactctta tttcgcccgc actgaagaag gcaagcagta tccagtgcgc tgccggcggc
     1681 gtgctgcgga cggcgcgccg gaagaattgt tgttggattt gaatgcgctg gcggaaggcc
     1741 actcgttttt ggggctcggt gcttttgagg tgagcgacaa taacgacctg ctggcctatg
     1801 cgacggatac tacaggattt cggcagtacg ccctgcaagt aaagaaattg gcgagtggcg
     1861 agacgctcgg cgaacgattc gagcgcgtga ccagcgtggc ctgggctgcg gacaaccgta
     1921 cgctgttttt tactgaggaa gatgagacca ccaagcggtc acatcgtttg taccggcatg
     1981 ttttgggttc gggcgaagcg ccgattttgc tttacgaaga aaaagatgag cgattccgca
     2041 tcggtgtgga acgcactcgc agcggaagct ttctactgtt gacgagcgcg agtcacacca
     2101 cgagcgaagt gcggtttcta tctgcgaagc atgcgggcgg caatttttct ctgatcgaac
     2161 cgcgcgaaga taaccacgaa tattatgtcg agcatcatcc gggcgatgag gactcctccg
     2221 acgccggagt attttatatc cgcactaatt ctggcgggcg gacattccgg ttgatggaag
     2281 tttcgaccaa ggagccgtcc agggaattgt ggcgcgaggt gattcctaac cggccagaag
     2341 taatgctggc gggcgcagat gcgtttgaaa atcatctggt gctatttgag cgcgagggcg
     2401 ggctgcctta tctgcgcatc atcgatcttg aaaatattgg cgacgaaagc gaagaccgct
     2461 acggtgattt gctgaacgat tcgcatcgca ttgaatttac ggagccggcg tacaacgctt
     2521 cgctgggcac gaatcctgag tttgttacca attttgtgag gtttcaatac gagtcatttg
     2581 taacaccgcg ttccgtgttt gattacaacg tgcccacgcg tgagcgactt ttgctgaaac
     2641 agcagccggt gcttggtggg tacgatgcgc aacgatacgt cacagagagg ttgcatgcta
     2701 ccgccgcgga cggaacacag ataccaattt cagtcgtata ccggcgggac acttttgctc
     2761 gcgagcctaa atggccgacc gaccgcagca cggacgcgcc gttgctgctt tatgggtatg
     2821 gaagttacgg tatctccgtg cccgttacgt ttagttccaa tcggttgagc ttgctggatc
     2881 gcggcgtcgt ttttgctatt gcgcatatac ggggcggtgg cgaattgggg aaaccctggc
     2941 atgatgccgg gcggatgcgc cagaagatga ataccttcac ggattttatt gccgcggcgg
     3001 aatttctaat cgcgcaacga tatacgtcac cggaaaaatt agtgatcgag ggcggcagcg
     3061 ccggcggact gttaattggc gcggttgtga atttgcgtcc gtatctattt cacgctgcga
     3121 tttcacatgt gccgttcgtg gacgtgttga acacgatgct ggacgcttcc ctgccgctga
     3181 ctgtgggcga atatgaagag tggggcaatc cgcaggtggc cgaagattat tgggttatga
     3241 aatcgtattg tccgtatacg aatgtgacgc gctgtgcata tccggcgatg ctagtgaaaa
     3301 cgggattgaa tgacagccag gtgatgtact gggagcccgc gaagtatgtg gcgaagttgc
     3361 gcgccatgaa gacggacacg aatccattgt tgttcaaggt gaatatggga gccggacatg
     3421 gcggcgcttc gggaagatac gattatctgc gcgaggtggc gctggactat gcgttcgtgc
     3481 tgacgcaatc gggaatccgc gagtgaatcg gagcgaggga ttggcgcata gttcctctgc
     3541 gttagcgcgg cggggcgccg cagaggcgcg tgtgcggatg cctcgcacaa gatgtggctt
     3601 acttccgtga tcgaagctac tactccgctc atgcagcagt atcaggggat taagtcacgg
     3661 tatccgcacg cgctactgct tttccggctc ggcgatttct acgaactgtt ttacgaagac
     3721 gcgattatcg ctgcgcgcga actgcaaatc acacttactt ccagaaacag ggaaaagggc
     3781 cagcccattc cgatgtgtgg cgttccttac catgccgccg acgggtatat cgctcggctg
     3841 attcgcgcgg gcttcaaaat tgccatttgc gaccagatgg agttgccggg cccgggcagg
     3901 aaaattgtgc ggcgggaagt ggtgcgtgtc atcacgcccg gcacggccac agatggcgca
     3961 gtgttggaag cgcgtgaaaa taattttctg gcggcggtag ccaaggatgc cggcggcacg
     4021 ttgggaatgg cgtatgtgga cttgtccacc ggagaatttc aggccacgga gtttagcggg
     4081 cctggcgccg ccgaagcgtt gcgcgatgaa ctgcaattgc tgaggccacg cgaaacgttg
     4141 ctgccccgtc cgcaacaatt gtttgagact gcgaagactt ccctgctaga tgggaccggc
     4201 ggtgttgaat cgcggctcga tgaatggatt tttcagcacg attacgcgga gcgaattctg
     4261 cgcgaacagt ttggcgtggc ggagttgacc ggctttgggc tggatgggca ccagcaatcg
     4321 atggcggcgg cgggcgcgat cattcattac ctgcgcgaaa acgcagcgcg gggggatgat
     4381 gcgcatagcg tggaggcgct gcgtcatttg gatggcgtgc gttattacga gcagcacaat
     4441 gctctggtgc tcgatcctgt ctcggtgcgc aatctggaat tgatcaaccc aatttttact
     4501 gaggagagcg gccgtgcggg gccgactacg ctgattgccg cgctggatgt gacggttacg
     4561 ggaatggggg cgcggttgtt gcggtcctgg atgatgcggc cgctggtcga ccccgtggcg
     4621 attgaggcgc gattaaatgc agtggcgcat ctggtgcagc agaccatggt gcgtggagag
     4681 attcgcaagg aactaaaagg catcctcgat ctggagagga ttacgagccg cattacgctt
     4741 ggtctagcta ctccccgtga attagtagcg ttgcgcaaat cgctggcccg gatccccttg
     4801 cttaagaatt ttttgactcc tccctctgcg ggcggcagcg aattactgcg gcggattttt
     4861 actgagattg atgagctgag cgacgtgcgt gaacgattgg agcgcgggat ttccgatgag
     4921 ccgccggcgc tggcaactac gccgggaatg attcgcagcg gttttgatgg cgagctggat
     4981 gaactgcgca acttgagcca gcacagcaaa gaaattatcg ctgcgatgga agaacgggag
     5041 agaaagcgaa ctggtatcgc ctcgctgaag attcgcttta attctatttt tgggtactac
     5101 atcgagattt ccaaatcgaa tctgcatctt gcgccagcgg attacgaacg caagcagacg
     5161 ctggtgaatg ccgaacggtt tacttcggcg gaactaaaag agtacgagcg gcaaattctg
     5221 gcggcggacg aacgcatcgc ggagatcgag aaccggctat ttgtggaatt gcgctcggga
     5281 gtggcgctgc atgcggcgcg gctgcgcgtg acggccagtg cgattgcgca actggacgtg
     5341 ttgacttcct ttgccaagtt ggctgcggaa cgcggatatt gtcgaccgga atttaatgct
     5401 accggagagc tgctgattgt tgatgggcgt catccggtga ttgaggagtt gctgcggcaa
     5461 cgtggcgagc gatttgtgcc gaatgatttg tatcttgaat cgcagcggca acaattgtta
     5521 ctgatcaccg ggccgaacat gggaggcaag tcgacgtact tgcggcaagc ggcgctgatt
     5581 gtattgatgg cgcagatggg atgcttcgtg ccggcgcggc aggcgaagtt gccggtgacg
     5641 gacagaattt ttacgcggat tggagccagc gataatctgg cgcgcgggcg gtcaaccttt
     5701 ttggtggaga tgagcgaagt ggcgactatt ttgcatcacg caacgccgtc gagtctggta
     5761 ctgctggatg aagtggggcg cgggacttca acatttgatg gattgtccat cgcctgggcg
     5821 gtggtggagc atttgcagaa gcacaccgga gcgagaacac ttttcgctac tcattatcat
     5881 gaattgacgg aattggcgga gctgctttcg gcggtgaaaa acgtacatgt gtcggtgaaa
     5941 gaaacgccga atgaaattgt ttttttgcgg cgagtggagc ctggaagtgc ggataagagt
     6001 tatggaatcg aagtggcgcg acttgcgggt ctgccgcgca gcgtgattga acgcgcacgc
     6061 gaggtacttt tgaagcacga gcagaatgag catgaactaa gtgagacgct atcgcctgcg
     6121 gtgaacggga atgggagtca caacgggagc caggcggtgt tgttcacggc gttagatcgc
     6181 gaagttattg agaaattgcg cggagcggac ctggatcaac tgaagccgct ggatgcactg
     6241 aatctgctgg cggaattgaa gaagcagatt tcatgagcac cggcgcgaca aaaggcggaa
     6301 tgctcgtgct gggactgatg tccgggacgt cggcggacgg aattgatatc gcgctggcga
     6361 gcgtttcggg agcaccgccg cgagtggagg cgcgactcct gaatcacacc gctgtgaaat
     6421 ttccggcgcg ggtgcgcgct gagattctgc gtgtggcgga gcagcgtgcg attaccgcgg
     6481 gagaattgag ccagcttaac tttcggctgg gagggttatt tgccgatgcg gcgaagactg
     6541 cttgcaaacg ctttcgcgtg gcgctttcct cggtggattt aattggcagc cacgggcaga
     6601 cgattttcca tcagggggtt ccggcgaatt acctcggcgc accgactgct tccactttgc
     6661 agattggtga agcggcggtg attgcggcgg ggactggcgt tactactgtg gctgattttc
     6721 gtcccgcgga tatggctgtt ggcgggcaag gcg
//
LOCUS       DATCBB010000033         1244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_33789, whole genome shotgun sequence.
ACCESSION   DATCBB010000033 DATCBB010000000
VERSION     DATCBB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33789"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VII37_00430"
     CDS             complement(<1..893)
                     /locus_tag="VII37_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068209.1"
                     /translation="MNEELIYDWNLASPPAIPPGTRVLLNDETLRDGLQNPSVFDPSI
                     EEKIAILHLMEALGIDSVNIGLPGAGARAVADTEALAREIASKRMKIKPNCAARTVEA
                     DILPIVEISQRVGIAIEAATFLGSSPIRRLVEDWTVDHLERTTEKAVRFAVEHGLPSM
                     YVTEDTIRTDADSVKRLYSCAIRNGARAIVLCDTVGHATPSGAYNLVKFAVDEVVKPS
                     GENIRVDWHGHNDRGLAVANSLAAIAGGANQVHAAANSLGERVGNTAMELMLVNLRLM
                     GLIGQDLSRLKEYCERVALAT"
BASE COUNT          296 a          407 c          275 g          266 t
ORIGIN      
        1 tgcgtcgcca acgccactcg ctcgcaatat tccttcaagc gcgaaagatc ctgcccaatt
       61 aatcccatca accgcaaatt aaccagcatc aactccatcg ccgtattccc gactcgctcg
      121 ccaagcgaat tcgccgccgc gtgcacctgg ttcgcgccac ccgcaatagc agccagcgaa
      181 ttggccaccg ccaatccgcg atcgttatgc ccgtgccaat ccacgcgaat attttcgccc
      241 gaaggtttca caacttcgtc caccgcaaat ttcaccaggt tgtaagctcc gcttggtgta
      301 gcatgcccca ccgtgtcgca cagcacgatt gctcgcgcac cgttgcgaat cgcgcagcta
      361 tacagccgct tcaccgaatc ggcatccgtg cgaatggtgt cttcggtcac gtacattgac
      421 ggcagcccat gctccacggc aaatcgcacc gctttctccg tggtgcgttc cagatgatcg
      481 acggtccaat cctccaccag cctgcggatc ggactcgacc ccaaaaacgt cgccgcctca
      541 atcgcaattc ccacccgctg cgatatctcc acgatcggca aaatatccgc ttccacggta
      601 cgcgccgcgc aattcggctt gatcttcatc cgcttactgg caatttcgcg cgccaaagcc
      661 tcagtgtccg ccacagcccg cgcacccgcg ccaggcaatc caatatttac ggaatcaatt
      721 cccagcgctt ccatcaaatg cagaattgca attttttctt cgatgctagg atcaaaaaca
      781 gaaggattct gcagaccatc tcgcaacgtc tcatcgttca acaacacccg cgtgcccggc
      841 ggaatcgccg gtggcgaagc caaattccag tcgtaaatca attcctcatt cattctcgtt
      901 tactctaccc gatttcgagc ggtttcgaat cgcacaacgc aatgtaacac gcgtgacaat
      961 caagtacaga tcatgctcgt tccagtcggc aacccaatgc gcgagccgag gggtctctcc
     1021 acagtgggaa gcaaatttta cgaataccct taactcgctc gggcatgaga cagcaaaatt
     1081 gaccacttct tcaggttcta gacatcacgt tcgcgagggt gtatgaagat tcacggtgcg
     1141 attagcgctg acagcgctca accaagatta gggagaggaa atcaggccca ggcttggcgc
     1201 accattccga tcgtggtctg cagaacgtat ctagcgaata cctg
//
LOCUS       DATCBB010000034         1091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_228291, whole genome shotgun sequence.
ACCESSION   DATCBB010000034 DATCBB010000000
VERSION     DATCBB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228291"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..>1091
                     /locus_tag="VII37_00435"
     CDS             80..>1091
                     /locus_tag="VII37_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068210.1"
                     /translation="MYLLQCAWLVRVQAMNGSLPDSDQALRMYQGLEQWQGGAIAGTP
                     ESLRAEAATGVPSAVRSGHLRVRDGYDQDRSPLYYLVAAVPLLVPLSTKLPEVFVPFL
                     AAAPQMFFGVMLGASLWYVARRLYGNAGGYIALGLYCFSPAMIVNAAGAQSLGEMGAV
                     WGAFGMIFTAIAVAHTLYAPREVVLWNWRRILLLGLSIALAVSNQFSLVVLVLVILPL
                     MLWVAPVRPRAVLVIWIVAIAVAAVLMLAAYCFEPMLFWQGLLHARWMDFEPIAFGMP
                     ISYRKVAQNIIGGSPPLILALPVAVIIYFGWKRARYFGNTAPLAGVGMFVILAVGAFG
                     FPG"
BASE COUNT          160 a          281 c          363 g          287 t
ORIGIN      
        1 tcggtctgcc ttgttgcgcc gaattcttca ttccgctgct tttcgcgtcc cgcaatttgc
       61 ggccgctgcg ctgttgctga tgtatctgct gcagtgcgcc tggctggtcc gcgtgcaggc
      121 gatgaatggg tcgctgccgg attccgatca agcgttgcgc atgtaccagg ggctcgaaca
      181 atggcaggga ggcgccattg ccggaactcc ggaatcgttg cgggcagaag ctgctaccgg
      241 cgtgccatcc gcggttaggt ccggccattt acgggtgcga gacggttacg atcaagaccg
      301 gtcgccgctt tattatctgg tggccgcggt gccgctcttg gtgccgctaa gtacgaaact
      361 gccggaagtt tttgtgccat tcctggctgc ggccccgcaa atgtttttcg gcgtgatgct
      421 gggagcatcg ctgtggtatg ttgcgcgccg actttacggc aatgctggag gctacatcgc
      481 gcttggactc tactgttttt cgccggcaat gatcgtgaat gcggcgggcg cgcaaagtct
      541 gggggaaatg ggggcggtgt ggggagcgtt cgggatgatc tttactgcaa tcgcggtggc
      601 gcacacgctt tatgctcctc gcgaagtcgt gttgtggaac tggcggcgga ttctgctgct
      661 gggattatcg attgcgttgg ctgtgagcaa ccagttttcg ctggtcgtgc tggtgttggt
      721 aatcttgccg ctcatgctgt gggtggcgcc ggtccggcct cgcgcggtgc tagtaatctg
      781 gatcgtcgca attgcggttg ccgctgtgct gatgctggcc gcctactgtt ttgagcccat
      841 gctgttttgg caagggttac tgcacgcgcg gtggatggac ttcgaaccga tcgcatttgg
      901 catgccgatc tcataccgta aagttgcgca aaatatcatc ggaggcagtc cgccgctgat
      961 actggccttg ccagtcgcgg taatcatata tttcgggtgg aagcgcgcgc gctattttgg
     1021 caacaccgcg ccgcttgccg gggttggcat gtttgtgatt ttggcggttg gtgcattcgg
     1081 ctttccggga c
//
LOCUS       DATCBB010000035         1596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_423101, whole genome shotgun sequence.
ACCESSION   DATCBB010000035 DATCBB010000000
VERSION     DATCBB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423101"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VII37_00440"
     CDS             <1..233
                     /locus_tag="VII37_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY5068211.1"
                     /translation="EAGNLALKVLALGGVFVAGGIAVKILDKIKDGSFFQSFQDKWKF
                     EPLMNNIPVSVILNESAPLIGAAYEALAAMQK"
     gene            256..807
                     /locus_tag="VII37_00445"
     CDS             256..807
                     /locus_tag="VII37_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068212.1"
                     /translation="MAFLEEHIGPWKAVSRGAAIGWLVFYLIFFLYAAFNHSGFLFLD
                     YVNLIIHEGGHFFFSWFGYTITILGGTLGELIVPLLCGLYFFRQREVTAVTFCSFWFF
                     ENFLYIGTYMADARALALPLVGSGDHDWEILFGQWGLLQQDQRIGGITREIGWLGMLL
                     TVVWLVWRTRLLSSEQTSSQLVL"
     gene            926..>1596
                     /locus_tag="VII37_00450"
     CDS             926..>1596
                     /locus_tag="VII37_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5068213.1"
                     /translation="MSSTVTTQAPDLQNYVHGGWRRSATREYVEVLNPATAELLARTP
                     LSTAADVDAAVQAAADAFAAWRRTPPGERVQYLFKLKNLLEEHIGELARLITEENGKT
                     LAEAKAELRRGIENVEVACGIPMMMQGYNLEDVTPGVDEMLIRQPLGVVAAIVPFNFP
                     AMISFWFLPYAIACGNTFVLKPSERVPLTMRRIIELVEATGLPRGVVNLVNGGREVVD
                     ALCDHP"
BASE COUNT          332 a          378 c          468 g          418 t
ORIGIN      
        1 cggaagcggg aaatttggcg ttaaaagttt tggcgctcgg cggtgtgttc gtggccgggg
       61 gaattgccgt aaaaatactg gacaagatta aagatggttc gttttttcag tccttccagg
      121 acaaatggaa atttgaaccg ctgatgaaca atattcccgt gtccgtaatt cttaacgaat
      181 cggcgccgct aattggcgcg gcctacgaag cgcttgcggc catgcagaag taacggtcgc
      241 taagtaaaag gtcccgtggc atttctggaa gagcacatcg gcccctggaa agcggttagc
      301 cgtggggcgg ctatcggctg gctggtgttc tacctaattt tctttttgta tgcagcgttc
      361 aatcattctg gtttcctgtt tctcgactac gtcaacctga tcattcatga gggcgggcat
      421 tttttcttta gctggtttgg ttacacgatc accatccttg gcgggaccct tggcgaattg
      481 atcgtgccac tattgtgtgg gctctatttt ttcaggcagc gcgaggtgac tgcggtaact
      541 ttttgtagct tctggttctt tgagaatttt ctgtacattg gcacgtatat ggcggatgcg
      601 agagctttgg cgttgccgct ggtcggctcc ggcgatcacg actgggaaat tttgtttggc
      661 cagtggggtt tgctgcagca ggatcagcgt atcggcggaa ttacgaggga gataggttgg
      721 ttgggaatgc tcttgactgt cgtttggtta gtgtggcgaa cgagattatt atcctccgaa
      781 cagacctcgt cacaacttgt gctctagtcc gtggcgcggc tgcctcttgc agttttttgg
      841 tagactacca tcgtccgttc tagcgcgctc cgctccgcga ttgacgtaag tattgtcagt
      901 tactgtaaac attaaggaaa acaacatgag tagcacagtc acaacacaag ctcccgattt
      961 gcaaaactat gttcacggtg gatggcgccg ttccgccact cgagaatatg tggaggtatt
     1021 aaatcccgcg accgctgaat tgctggcgcg cactccgcta tccacggctg cggacgtaga
     1081 cgcggctgtg caggctgcgg cggatgcatt tgcggcatgg cgacgcacgc cacctggtga
     1141 acgcgtgcag tatctgttta agttaaaaaa tctgcttgaa gaacacatcg gcgaactcgc
     1201 gcgcctgatc accgaggaga acggcaaaac tctggcggaa gcgaaggcgg aactacgccg
     1261 aggcatcgaa aatgtcgaag tggcctgcgg aatccccatg atgatgcagg gctacaatct
     1321 tgaggatgtt acgccgggcg tggatgaaat gttgattcgc cagcccctgg gagtcgtcgc
     1381 cgcaatcgta ccgtttaatt ttccggcgat gatttcgttt tggtttctgc cgtacgccat
     1441 cgcctgcggc aacacatttg ttctaaagcc gtccgaacgt gtgccgctga cgatgcgtcg
     1501 cattattgag ctggttgaag ccacgggctt gccgcgcgga gtcgtgaact tggtgaatgg
     1561 cgggcgcgaa gtggtggatg cactgtgcga tcaccc
//
LOCUS       DATCBB010000036         1628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_163766, whole genome shotgun sequence.
ACCESSION   DATCBB010000036 DATCBB010000000
VERSION     DATCBB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163766"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="galE"
                     /locus_tag="VII37_00455"
     CDS             <1..1076
                     /gene="galE"
                     /locus_tag="VII37_00455"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY5068214.1"
                     /translation="VLHQHFSNSIDVAAVGYSCCSYAQEYSLNVLVTGAAGYIGSICS
                     EVLLARGINVIALDNLQEGHREAVPRQAVFCHADLGVRSQIEEVFAQHKIDAVMHFAG
                     EALVAKSVREPSTFYAANVAGGVNLLDAMIRHGIKKLIFSSTAATYGEPEMVPIPENH
                     PKSPINPYGKSKLVFEQILADYRAYTKLAYVTLRYFNAAGASEQRGEAHRVETHLIPR
                     VLDAASGAIPHVDVLGTDYPTPDGTCVRDYIHVLDIADSHLRALQEIERVAGESFNVG
                     NSRGYSILEVIEVAERVTGRKIPRQLSPRRPGDPAVLVASKEKLKTMLGWEAAHSSLE
                     EIISSAWNWKQKHPRGYAEAVTA"
     gene            1164..>1628
                     /locus_tag="VII37_00460"
     CDS             1164..>1628
                     /locus_tag="VII37_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068215.1"
                     /translation="MPTFYIEQFGCRATQADGAALEVQLRERGYTPAAKAAADIVVVN
                     TCTVTAAADAQARDAIKKISARNHAARLIVTGCYAQRAPEEVAQLPGVAWVVGNSHKP
                     ELPAIIENLSIAPTSKDFVPVLSLHMAHRALHQPAQILTGGFDRKPLLSAPVF"
BASE COUNT          370 a          482 c          416 g          360 t
ORIGIN      
        1 gcgttttaca tcagcatttt tccaattcaa tcgatgtcgc agccgttggc tattcgtgct
       61 gctcatacgc tcaggagtat tcattgaacg ttctggtcac cggggctgca ggctacatag
      121 gtagcatttg cagcgaagtg ctgctggcgc gcggaataaa cgtaattgcg ctggataatc
      181 tgcaagaggg ccatcgcgaa gctgttccgc gtcaggccgt cttttgccat gcggatctcg
      241 gcgtacgctc gcaaattgaa gaagtgttcg cccaacataa aattgacgcg gtgatgcact
      301 ttgccggcga agccttggtc gccaagtccg tccgagaacc cagcaccttt tacgccgcaa
      361 acgtagccgg tggcgtgaat cttctcgacg ccatgattcg tcacggaatc aaaaagctta
      421 ttttttcgtc cactgcggcc acctacggcg aacctgagat ggtgcccatc ccggaaaatc
      481 accccaaaag tcccatcaat ccctatggca aatccaagct ggtattcgag caaatcctcg
      541 ccgattatcg tgcgtacacc aagctggctt acgttacgct gcgctatttc aatgccgctg
      601 gcgcatccga acagcgcggc gaagcgcatc gtgtcgaaac ccatcttatt ccccgagtgc
      661 tggacgcggc gagtggcgcg atcccccatg ttgatgtcct tggcactgat taccccaccc
      721 ccgatggaac ttgcgtccgc gactatattc acgtcctcga tatcgcggat tcccacctgc
      781 gcgcgcttca ggagattgag cgcgtcgccg gtgaatcctt taatgtcggc aatagtcgag
      841 gctattccat tttggaagtt atcgaagttg ctgagcgcgt caccggtaga aaaatcccac
      901 ggcaactttc tccgcgcagg ccgggtgacc ccgccgtcct ggtcgccagc aaggaaaagc
      961 tgaaaaccat gctgggatgg gaagccgccc actcgtcgtt ggaagagatt atttcctcag
     1021 cgtggaattg gaagcagaag catcctcgcg gttacgccga agcggtcacc gcataaggaa
     1081 tcagcagata aaacgtggcg ccggcactga ctacttcgcc gtgagaccgt ctgttaaaat
     1141 tcccgtgtca taaattctaa atcatgccga ctttttacat tgagcagttc ggatgtcgcg
     1201 ctacgcaagc cgatggcgcc gcgcttgaag tccaattgcg ggaacgaggt tacacgcctg
     1261 cagccaaagc tgcagccgac atcgtggtcg taaacacctg caccgttacc gccgctgcgg
     1321 acgcacaagc ccgagacgcc atcaaaaaaa tcagcgcgcg aaatcatgcc gcgcgcctaa
     1381 ttgtcaccgg atgctacgcc cagcgcgctc ccgaagaagt ggcccaactc ccaggcgttg
     1441 cctgggtagt agggaattcg cacaagccag aattgcccgc aatcattgaa aatctttcca
     1501 tcgcaccgac ctccaaagat ttcgttcccg ttctttcctt gcacatggct caccgcgctc
     1561 tgcatcaacc ggcgcaaatt ttaacgggcg ggtttgaccg caaaccgctc ttaagtgctc
     1621 cggtattc
//
LOCUS       DATCBB010000037         1602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_423171, whole genome shotgun sequence.
ACCESSION   DATCBB010000037 DATCBB010000000
VERSION     DATCBB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423171"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1602
                     /locus_tag="VII37_00465"
     CDS             <1..>1602
                     /locus_tag="VII37_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5068216.1"
                     /translation="KAGAKLMDFGLAKPSVAMTAASDLLTPSGPTMSVAALTAATSPL
                     TQKGMIVGTFQYIAPEVLQGKEADARSDIFSLGCVLFEMATGKRAFEGKSQLSVFTAI
                     LENEPPLVSSQQPVAPKALDEIVKNCLAKDPEERISTAHDVKLQLRALEPRAGSGVIA
                     APAKTGIDRRVQNILLGAGTLALLAAALLGYALVGRKSDGGALRLQIAPPEKIKFVDT
                     GDYGGMPVLSPDGSKLAFSAEGENSPNALWVRSLDSFAAQRLEGTDEAAHPFWSPDGR
                     YIGFFAKGKLNKVLASGGPVIALATAYNPRGGTWCSSDVIVYADNFQSGLMKVNANSG
                     APSGATKLDATKHSTHRWPWCLPDGKHFVYLATNHQGGTPAENGVYYAGLDGKDVHQV
                     VPSDAGAEVADGYLLYHSQATLMAQPFDEANGTVRGEPVAVLNKVKFDTGVWRALFTV
                     AQNGRIAYFTGGDAAVGTQLVKYDRSGKVLKMLGELGSYMDPTYSPDGKLLAVLTGDP
                     LWNVWIMDLERGSRTRVTFDPTVKT"
BASE COUNT          358 a          380 c          572 g          292 t
ORIGIN      
        1 gaaggccggg gccaagctga tggatttcgg gctggccaag ccatccgtgg cgatgactgc
       61 ggcgagcgac ctgttgacac cgagcggtcc gacgatgagc gtggcggcgc tgacggcggc
      121 gacgtctccg ttgacgcaaa aaggaatgat tgtcgggaca tttcagtaca tcgctccgga
      181 ggtactgcaa ggcaaggagg cggacgcgcg gagcgacatc tttagtttgg gatgtgtgct
      241 ctttgagatg gcaaccggga agcgagcgtt cgaaggaaag agtcagttga gcgtgttcac
      301 ggcgatcctg gagaacgagc cgccgctggt gagcagccaa cagccagtgg cacctaaagc
      361 attggatgaa attgtaaaaa actgcctggc gaaggatccg gaggaacgga tttcgacggc
      421 gcacgatgtg aagctgcaat tgcgcgcttt ggagccgcgg gcgggaagcg gagtgatagc
      481 cgcgccggcg aagacgggga tcgatcgccg ggttcaaaat attctgcttg gggcggggac
      541 cttggcgttg ctggctgcag cactgttggg atatgcgctc gttggaagga agagcgacgg
      601 cggagcattg cggctacaaa ttgcgccgcc ggagaagatt aagtttgtgg acaccggcga
      661 ttatggagga atgccggtat tgtcgccgga cggaagcaag ctggcgtttt ccgcggaggg
      721 cgaaaacagc cccaatgcgc tgtgggtgcg ttcattagat agcttcgcgg cgcagcgact
      781 ggaaggaacg gatgaggcgg cgcacccgtt ctggtcaccg gacggacggt acatcggatt
      841 tttcgcgaag gggaaattga acaaagtgct agcgagcgga ggcccagtga tcgcgttggc
      901 gactgcttac aatccgcggg ggggaacgtg gtgcagcagc gatgtaatcg tttatgcgga
      961 caatttccag tccggattga tgaaggtgaa cgccaatagc ggtgcgccga gcggagcgac
     1021 aaagttggac gccacgaagc actccactca ccggtggccg tggtgcctgc cggacgggaa
     1081 gcatttcgtt tatcttgcca ccaatcacca agggggaacg ccggcagaga acggagttta
     1141 ctacgcgggg cttgatggaa aagacgtgca tcaagtggtg cccagcgacg ctggggcaga
     1201 ggttgccgac ggctatctgt tatatcactc gcaggcaacg ctgatggcgc agccgtttga
     1261 cgaggccaac ggaacggtga gaggggagcc ggtggccgta ctgaacaaag tgaaattcga
     1321 cacgggggta tggcgggctt tgtttacggt cgcgcagaat gggcgcatcg cctatttcac
     1381 gggcggggac gccgcagtgg gaacgcaact ggtgaaatac gatcgcagcg gcaaagtgct
     1441 gaagatgctc ggagagctgg gctcgtacat ggatccgacc tattcgccgg atggaaaact
     1501 gctggcggtc ctgaccgggg atccgctgtg gaacgtgtgg atcatggatt tggagagagg
     1561 cagccggaca cgcgttactt tcgacccgac ggtaaagacc ag
//
LOCUS       DATCBB010000038         1083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_98936, whole genome shotgun sequence.
ACCESSION   DATCBB010000038 DATCBB010000000
VERSION     DATCBB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98936"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VII37_00470"
     CDS             <1..855
                     /locus_tag="VII37_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068217.1"
                     /translation="IRLAPNQSANVKFSIPLVASTKSANGIAQRFKWPHQIVFEAQRA
                     SGKAAWQTIGESIPVVLSYADARVAPNLRVGYIRGFDFSLPNALAALGVESKELSVDE
                     VKTADLSKYTSIIVDNRVYESQPELIAANQKLLDYAKDGGTLIVFYHKSNEWNPDERR
                     KRPQLAPYKLILGDERITDETAPITFIEPDHPLLNFPNKMGQEDFANWIPERGLYYPK
                     DWDAQYHALLQSNDPGEAPLKGGLLVADYGRGHYIYTSMVWYRELRGGVPGAYRMLAN
                     MISYGHSK"
BASE COUNT          250 a          299 c          302 g          232 t
ORIGIN      
        1 atcagactcg cacctaatca gagcgcgaac gttaagttct caattccctt agttgcctca
       61 acaaagagcg caaacggcat cgcgcaaaga ttcaagtggc cacatcaaat tgtttttgaa
      121 gcgcagagag cgtccggtaa ggcagcgtgg caaacaatcg gagaatctat ccctgtcgta
      181 cttagttacg ccgacgcccg cgtcgctccc aacctccgcg ttggttacat ccgcggcttt
      241 gatttcagtt tgccgaatgc actggcagcc ctcggagtgg aatcgaaaga actaagcgtg
      301 gatgaagtga agacggcgga cttgtcaaaa tacacttcga tcatcgtcga caatcgcgtc
      361 tatgaatcgc agccggaatt gattgccgcg aatcagaagc ttttggatta tgcgaaagat
      421 ggcgggacgc tgatcgtttt ctatcacaag tcgaacgagt ggaatccgga cgagcgccgc
      481 aagcggccgc aactcgctcc ttataaactt attctgggcg atgaacgcat cacggatgaa
      541 acggcgccga taactttcat cgagccggat catccgctgc tcaactttcc caacaagatg
      601 ggtcaggaag acttcgcgaa ctggattccg gagcgcgggc tttactatcc gaaagattgg
      661 gacgcgcagt atcacgcgct gttgcaatca aatgatcccg gcgaggcgcc gctaaagggt
      721 gggttgctcg tggccgatta cgggcgcggg cattacatct acaccagcat ggtttggtat
      781 cgcgagttgc gcggcggcgt gcccggcgcg taccgcatgc tggcgaatat gattagttat
      841 gggcactcga aatgaattgg agttcttgcg gctctgccgc ctgatgtgcg gaaagcctac
      901 gactttccga ccgcacgcct tccctgtttg ctcattgcgg cgaggcagag ccttaccgca
      961 catcgggcgg caaagccgca aaccatttcg cgcttcgcgc tcaaatgcgg gcgagggcgc
     1021 ccgcgctccc agtatcaagt tcagtgctca gaccaggcag ccgcgttccc agcatccgag
     1081 gtt
//
LOCUS       DATCBB010000039         1267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_261217, whole genome shotgun sequence.
ACCESSION   DATCBB010000039 DATCBB010000000
VERSION     DATCBB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261217"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1267)
                     /locus_tag="VII37_00475"
     CDS             complement(<1..>1267)
                     /locus_tag="VII37_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5068218.1"
                     /translation="PGAVVFPTTTHEVSRVVRFAAEHDIPIIGRGAGTGLSGGALART
                     GGIMIVFARMNQILELDLENQRAVVQPGVVNYELTRAAESAGLYFAPDPSSWKSCTIG
                     GNVSENAGGPHTLAYGVTSNHVTALELVLPDGEICRVGSRHGDSLGYDLAGLFVGSEG
                     TLALVTEITVKLLRKPESVKTLLGIFDRVDDASETVAEITARAITPAACEMLDGWTLR
                     AVEDFIHAGFPLDSAAVLLIELEGLAEVVAAQVSAVEEVCRTHHAREVRVARDEQERE
                     LLWKGRKNAFGALGRIAPSNYVLDGVIPRSKIPQALRRIQEIGERYNFKIGNIFHAGD
                     GNLHPIVLFDPRDAGQYQRAIEASAEIIRYCVELGGALTGEHGVGMEKSELMPLLFSD
                     DDFSLMRRIHDAFNPRSALNPGKIFPLNKG"
BASE COUNT          281 a          407 c          324 g          255 t
ORIGIN      
        1 acccttattc aacggaaaaa tcttcccggg attcagcgcg gatcgtggat taaacgcgtc
       61 atgaattcgc cgcatcaacg aaaagtcatc atccgaaaaa agcagcggca tcaattcgct
      121 tttttccatt ccgacgccgt gctccccggt aagcgcgccg ccgagttcca cgcaatagcg
      181 aataatctcg gcggaagcct caatggcccg ctgatattga cccgcatcgc gaggatcaaa
      241 caaaacgatg ggatgtaaat ttccatcgcc ggcgtgaaaa atgttgccaa tcttgaaatt
      301 atatcgttca ccgatttcct gaatgcgccg cagcgcttga ggaatttttg atcttggtat
      361 gacgccatcg agcacgtaat tactgggcgc gattcggccc agcgcgccga aggcattctt
      421 gcgccctttc cagagcagtt cgcgctcctg ctcgtcccgg gccacgcgca cctcgcgcgc
      481 atggtgcgtc cggcacacct cttccacggc tgaaacctgc gcggccacta cctccgccag
      541 gccctcaagc tcgatcagca gcaccgcggc gctgtccagc gggaaaccgg cgtggatgaa
      601 atcttccacc gctcgcagtg tccacccgtc gagcatttcg cacgccgcgg gcgtaatcgc
      661 gcgcgcggta atttccgcca cagtctcgga agcgtcgtcg acgcggtcaa aaatgcccag
      721 caaagttttc acgctttcgg gtttgcgcaa aagcttcaca gtaatttcag taaccagtgc
      781 aagtgtccct tctgagccta caaagagacc agccagatcg tagcctaggg aatcgccgtg
      841 gcggctgccc acgcgacaaa tctcgccatc cggcagaacc aactccagcg cggtaacgtg
      901 attgctcgtc actccatacg cgagcgtgtg cggcccacca gcattttccg aaacgttacc
      961 gccaatcgtg catgatttcc aactggatgg atccggcgcg aaataaagcc cggcgctctc
     1021 cgccgcgcgc gtcaattcat aattgaccac gcccggttga actaccgcgc gttgattttc
     1081 caggtcgagt tccagaattt gattcatgcg cgcaaagacg atcatgattc cgccggtacg
     1141 cgccagcgcg cctcctgaaa gccccgtgcc cgccccgcgc ccgatgatgg gaatatcgtg
     1201 ttccgcagcg aaccgcacaa ctcgcgacac ctcgtgagtc gtggtgggaa aaactactgc
     1261 acccggc
//
LOCUS       DATCBB010000040         1142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_1586, whole genome shotgun sequence.
ACCESSION   DATCBB010000040 DATCBB010000000
VERSION     DATCBB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VII37_00480"
     CDS             complement(<1..637)
                     /locus_tag="VII37_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068219.1"
                     /translation="MPLALTHPSRFQSGNLMIVSRTDPSLDSTRKIKPVRTSKHRERS
                     ADELVMEIESTMGESWLPGIYCDRILKLRTRSYVFPSIAKPSVPQIQHTLLGIELKVG
                     RKRMLCPDLATARYLAVFARAGCAAIAIPYDITKISHLADELESSWHRMLLLADSLAG
                     NRSEAFATRVRRLLIGKVRTAIAMAGAGPRIPEFTQSTRQGERPPESTESKK"
     gene            complement(594..>1142)
                     /locus_tag="VII37_00485"
     CDS             complement(594..>1142)
                     /locus_tag="VII37_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5068220.1"
                     /translation="ALVGFVAAGAATDAEQRSAALTSVAFYLLTYAVMNMGAFAIVTL
                     IARGNDRRTAVEDYNGIGFLSPVLAFSLSLFLLSLLGMPLTAGFMGKVMVFTTAVRQG
                     YLWLVVIGVLNTAVSAYYYLRLIIVMFFRERNAPWDAPRLPVSVALALVITIAGVFYL
                     GLFPGRVLNAFGAHPSISLSIR"
BASE COUNT          275 a          318 c          317 g          232 t
ORIGIN      
        1 tttttttgct ctcggtgctt tctggtggcc gctctccttg cctggtgctc tgtgtgaatt
       61 ccggaatccg cgggcccgcg ccggccatag cgattgcagt tcgtacttta ccaattaaca
      121 gtctgcgaac gcgagtggcg aaagcctcac tgcgatttcc cgccagactg tcggccagca
      181 gcaacatgcg gtgccatgaa ctttccagct catcagccag atgtgaaatc ttcgtgatgt
      241 cgtagggaat cgcgatcgct gcgcaaccgg cgcgggcgaa aaccgcgagg taacgcgccg
      301 tggccaggtc gggacaaagc atccgtttgc gaccaacctt caactcaatc ccgagcagtg
      361 tgtgttgaat ctggggtact gatggtttag ctatcgaagg gaatacgtat gaacgcgtgc
      421 gcaactttaa gatccgatcg caatagattc ccggcaacca cgattcgccc atcgtgcttt
      481 caatttccat taccagctcg tcggcagagc gttcccggtg ctttgatgtt cttactggtt
      541 ttatttttct ggttgaatca agggacgggt cggtgcggga gacaatcatg aggttaccgg
      601 attgaaagcg agatggatgg gtgagcgcca aaggcattga gcacgcgccc cgggaaaagg
      661 cccagataga aaactccggc aatggtaatt accaacgcga gcgcgacact gactggaagc
      721 cgcggcgcgt cccacggcgc gttccgctcg cggaagaaca tgacaatgat tagccgcaga
      781 taatagtacg ccgacacggc agtgttcaga acgccaatca cgaccagcca gagataaccc
      841 tgacgcacgg ctgtggtgaa caccattacc tttcccataa atcccgcagt caaaggcatc
      901 cccaaaaggc tcaacaagaa caacgaaagc gaaaaggcca gtactggaga cagaaagccg
      961 attccgttgt agtcttcgac tgcagtacgg cgatcgttgc cacgggcaat cagggtgacg
     1021 atagcaaagg cgcccatatt catcactgcg taggtcagca aataaaaggc taccgaggtc
     1081 agcgcggcac tccgctgttc cgcgtcggtg gccgcgcccg cagccacgaa tccaaccagg
     1141 gc
//
LOCUS       DATCBB010000041         1956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_325827, whole genome shotgun sequence.
ACCESSION   DATCBB010000041 DATCBB010000000
VERSION     DATCBB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325827"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VII37_00490"
     CDS             complement(<1..374)
                     /locus_tag="VII37_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY5068221.1"
                     /translation="MFLSFIITNFFFGFAWTEETRWWDYPGFELWKFLNLAIFVLALV
                     FILTRKAKLGEVFKARRESIKRELARAEQERDAALQKLKEVEARLGLLDKEVASIKEQ
                     SVREAAEERERIARSTEAEIVKL"
     gene            complement(377..856)
                     /locus_tag="VII37_00495"
     CDS             complement(377..856)
                     /locus_tag="VII37_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY5068222.1"
                     /translation="MVFPLGLAENSIQLVPDGTLLLHVLIILVMVYVLNATLFKPINR
                     ILAAREKRTRGRLTEAEEILRNVSEKLAEYERALRAARIEAYAFTERERAGELQDRQR
                     KLDEMRAKLAASTALEKEAIQHQAQAARGTLEVDARRIAVEIGALVLSRPITHADMN"
     gene            1124..1270
                     /gene="rpmH"
                     /locus_tag="VII37_00500"
     CDS             1124..1270
                     /gene="rpmH"
                     /locus_tag="VII37_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY5068223.1"
                     /translation="MPKRTFQPNNRRRAKTHGFRARMATKNGRLVIKRRRAKGRKRLT
                     PAHY"
     gene            1276..1680
                     /gene="rnpA"
                     /locus_tag="VII37_00505"
     CDS             1276..1680
                     /gene="rnpA"
                     /locus_tag="VII37_00505"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY5068224.1"
                     /translation="MAPSFDIAPVKVSEALPKRRRLHARAEFQKVYAEGQRYDGRLLA
                     AFLRKNELPEHRLGVTASTKAIGKSVDRNRAKRLLRELFRRSTNDLNGLQQAYDWVLN
                     AKRSLLVADEEHRFREFRKIIAQVTQAERRGV"
     gene            1819..>1956
                     /gene="yidD"
                     /locus_tag="VII37_00510"
     CDS             1819..>1956
                     /gene="yidD"
                     /locus_tag="VII37_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY5068225.1"
                     /translation="MRAILISLLKLYQAVISPWLPPSCRFVPTCSEYAREAIERHGAL
                     RG"
BASE COUNT          417 a          568 c          545 g          426 t
ORIGIN      
        1 agtttgacga tctcggcttc agttgaacgc gcaatgcgtt cgcgctcttc cgccgcttcg
       61 cggaccgact gttccttgat gctcgccact tccttatcga gcaagcccag gcgcgcttca
      121 acttctttca gtttctgaag cgccgcgtcg cgctcctgct cggcacgggc caattcgcgc
      181 ttgatgctct cgcgccgcgc cttgaaaact tcgcctaact ttgccttccg ggtgagaatg
      241 aagaccaggg cgagaacaaa gatcgccagg ttgaggaact tccacagctc aaagccagga
      301 tagtcccacc agcgcgtttc ttccgtccag gcgaaaccga aaaagaagtt ggtaatgata
      361 aaagacagaa acatcgttag ttcatatcgg cgtgcgtgat cgggcgacta agaacgaggg
      421 cgccaatctc gacggcgatg cggcgcgcat caacttccag cgtgccgcgc gctgcctgcg
      481 cctggtgctg aatggcttcc ttttccaacg ctgtagatgc ggccagcttc gctcgcattt
      541 cgtccagctt tctctgacga tcctgcaact ctccagcgcg ctcgcgctca gtgaacgcgt
      601 aggcctctat gcgcgccgcc cgtaaggcac gttcgtactc tgccagtttt tcgctcacgt
      661 tgcgcaggat ttcctcggcc tcagtcagac gaccgcgggt gcgtttctcg cgcgctgcca
      721 aaatgcgatt gatgggtttg aaaagagtcg cgttcagcac atagaccatc accaggatga
      781 tcagaacgtg caaaagcagc gtgccatcag gcacaagttg aattgagttt tcggccaagc
      841 cgagcggaaa caccatagga ttgttctaag ctggggaatt aagtttttcg atagtcgatg
      901 tagcgcaagc tgccagcttg cgcagactgg cagtctgcgc tacttaggcc gaaatcaaaa
      961 cgctaccgtt tcgtcagcta cagtgaccca actgcacgtg tcaaaagctt tgaaggctaa
     1021 cacgcgattt tataacgcgt caaggagcgt tgcggccttc cttgacatcg cattgcagcg
     1081 ccgttacgct aagtcttttc cctgaacgga ggaattattc tctatgccga agcgaacctt
     1141 tcaaccgaac aatcgccgcc gcgccaagac gcacggcttt cgcgcgcgca tggcgaccaa
     1201 gaacggccgc ctggtgatca aacgccgccg cgccaagggc cgcaagcggc tcactccggc
     1261 gcattactaa agccattggc cccgagcttt gatatcgcgc cggtgaaagt cagcgaggcg
     1321 ttgccaaagc ggcgccggct gcacgctcgg gccgagttcc aaaaagtata tgccgaaggg
     1381 caacgctatg acggccggct gctggccgct tttttgcgca agaatgaatt acctgagcat
     1441 cgtttgggag taacggcctc gaccaaggcg attggaaagt cggtcgatcg caaccgggcc
     1501 aagcgcttgc tgcgtgagct gtttcgccgc agcaccaacg acctgaacgg cttgcagcaa
     1561 gcgtacgact gggtcttgaa tgccaaacga tcgttgcttg tcgcagacga ggaacatcgc
     1621 tttcgcgagt ttcgtaagat catcgctcag gtaactcagg ccgaacggcg cggcgtttag
     1681 ttgtggagtg cgccggcaga gcgtagcggc gacggcgctt tggctaacag ttttggcaag
     1741 tggacaaggc aaataactgc ggatgaatcc aaagcggtgt cgcgctgcgc ttgccaccgc
     1801 actccaaagt cttagccgat gcgcgccatt cttatttcgc ttttgaagct ctaccaagca
     1861 gtaatctcgc cgtggctgcc gccgtcttgt cgttttgttc cgacttgctc tgaatatgcg
     1921 cgtgaagcaa ttgagcgcca cggcgcgcta cgcggg
//
LOCUS       DATCBB010000042         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_293718, whole genome shotgun sequence.
ACCESSION   DATCBB010000042 DATCBB010000000
VERSION     DATCBB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293718"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..869)
                     /locus_tag="VII37_00515"
     CDS             complement(51..869)
                     /locus_tag="VII37_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HEY5068226.1"
                     /translation="MANPEAEQGAHSESGEVPSSPSRPEAEPVNLHYDTRLPQLPWSR
                     RAQIPIIAAAVYSVIRTLGPTLRFEALGEQQPKRVYASGQRCIFAFWHRVIIPVAWWA
                     RNRGIVVMNTTAFDGQWTRKVIEWLGFGTAQGSSSRGGLRGLAVMAKRLEEGLDCGFT
                     IDGPRGPRYVAKPGPVMLARKSGCPILVFHIGVDRGKTFMQTWDHFLLPAPFARAVIL
                     CAPPIYVPADADADLMQAKHAEMQRELERVRDIAEGWFQLSEEERQRYRAEFLK"
     gene            936..1994
                     /locus_tag="VII37_00520"
     CDS             936..1994
                     /locus_tag="VII37_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068227.1"
                     /translation="MAFLLQQQNARRNVALFCSAATLAFFGIFAAAARPDNPSNGDSV
                     GSIEGEAIAVTGPMSVENVNGSVVTVLHSGSDVRVKSGSARINLVEGGQIAVCGPAHF
                     SVLKSGNSLTIALDTGTIHAHIEHKPALTMYTPQIQAQPVAIGDAPQDTIVGFDANGA
                     MCIRTNRGALRLEQQLTGQNVLVPQGGSVFLANGQIDSLRTDSGQHCACQLQIADAAP
                     PPPEVSQLASADEVRHKVEDVKPNAPSVEPAKPVAPKEQPVYQVFVPPLIFNANSKVQ
                     PEIDPKMIMLIRRVRVRPTLIFQGKVEGDVVPVKAPVAAPPRPATPPPATPPPVTKTA
                     SLMERVRTFFRKLWPFGS"
BASE COUNT          465 a          669 c          560 g          447 t
ORIGIN      
        1 aaaataaaat acaaaaggat ccggcccacg gcgttgaagc ggttcatgcc ctattttaaa
       61 aattcagccc gataacgctg ccgttcttct tcgctaagct gaaaccagcc ctcagctata
      121 tcgcgaacgc gctccaattc ccgctgcatt tccgcgtgct tcgcttgcat caaatccgcg
      181 tccgcatccg ctggcacata aattggcggc gcgcacagta tcaccgcgcg cgcaaatggc
      241 gctggcaaca aaaaatgatc ccaggtctgc atgaaagttt tgccgcgatc cacgccaatg
      301 tgaaatacca ggatcgggca tccactcttt cgcgccagca taaccggccc tggcttagct
      361 acgtagcgcg gcccccgcgg gccatcaatc gtaaagccgc agtcgagtcc ttcttccagc
      421 ctcttggcca ttacggccaa cccgcgcaat ccaccgcgcg acgagcttcc ctgtgcggtg
      481 ccgaatccca gccactcgat aaccttgcgc gtccactggc cgtcgaacgc cgtggtattc
      541 atcactacga tgccgcgatt cctggcccac cacgccaccg gaatgatgac gcggtgccag
      601 aacgcaaaaa tgcatcgctg gccggaagcg taaacgcgtt tcggttgttg ttcccccagc
      661 gcttcaaagc gcagcgtggg ccccagggtg cgaattacgc tgtaaaccgc ggcggcgatg
      721 ataggaattt gtgcgcggcg tgaccatgga agctgcggca accgcgtgtc gtaatgtaaa
      781 tttaccggct cggcttcggg tcgcgaaggc gatgagggca cctcgccgga ttcactgtga
      841 gcaccttgct ctgcttcagg gttagccatc gtcgtatagt ataggctgag agcctttcgc
      901 gagagtttcc gcggctcatt cgataggagc gaagcttggc gtttttgttg caacagcaaa
      961 atgcgcgtcg caatgtggcc ctgttctgca gcgccgccac ccttgcgttc tttggaatct
     1021 ttgcggcggc cgcacgtcct gataatcctt caaatggaga ttccgttggc tctatcgaag
     1081 gcgaagccat cgccgtcact ggccccatga gtgtggaaaa tgtcaacggc agcgtagtga
     1141 cggtgttgca cagcggcagc gacgtgcgcg tcaaatccgg cagtgcgcgt ataaatctcg
     1201 tggaaggtgg tcagatcgcc gtatgcggcc cagcgcattt ttctgtgctc aaatccggca
     1261 actcgctgac gatcgctctc gacaccggaa cgattcacgc gcacatcgaa cataagccgg
     1321 cgctgactat gtacacgccg caaattcaag cccaaccagt agctattgga gacgccccgc
     1381 aagataccat cgtaggattc gatgctaatg gtgcgatgtg catccgcacc aatcgcggcg
     1441 ccttacgcct cgagcagcaa ttaaccggtc aaaatgtctt ggtgccccaa ggcggaagcg
     1501 tttttctcgc aaatgggcaa atcgattcgt tacgcaccga ctccggtcaa cactgcgcct
     1561 gccagttgca gatcgcggat gcggcgccac cgccgcctga agtcagccag ctagcgagcg
     1621 ccgacgaagt gcgccataaa gtggaagacg tcaaaccaaa tgcacccagc gtcgagcccg
     1681 ctaagcccgt cgcaccgaag gaacagccgg tctaccaggt gttcgtaccg ccgttaatat
     1741 tcaacgcgaa ctccaaggtg cagccagaaa tcgatccgaa gatgatcatg ctgattcgac
     1801 gtgtgcgcgt gcgacccacg ctcatcttcc agggtaaggt cgaaggcgac gtcgtcccgg
     1861 tgaaggctcc cgtcgcggca cctccgcgtc ctgccacgcc gccccctgca actcctccac
     1921 ccgtaacaaa gaccgcttcc ttaatggagc gcgtgcgcac cttcttccgc aaattgtggc
     1981 ccttcggttc ttgagtccag gcccacccaa acctttactt tttgcatcaa accctttagt
     2041 cttaagaagt ccctgatgtc cccgcccagg gtcttctgta ccgtaaatcc tggcttttct
     2101 ccctctaagg ggaaccaagt catctgatca ggggaatttc c
//
LOCUS       DATCBB010000043         1282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_455786, whole genome shotgun sequence.
ACCESSION   DATCBB010000043 DATCBB010000000
VERSION     DATCBB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455786"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VII37_00525"
     CDS             complement(<1..604)
                     /locus_tag="VII37_00525"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEY5068228.1"
                     /translation="MTARWVRLVLGGLFGAVIIAGLYFPILKQRVKQTAKIQPQSEEQ
                     ARRELTQSLTATPTEARVKAKLFWASNAHDGSLTPVTIELPLSGDPALRAKQVLNTLL
                     AGPADLELRTLPPDAVLLAFYLLPDGTGIADFSEAMASSIPSGIESEQRAVDSITRTL
                     AANIPQIMRLKILIHGQEVETLAGHLDLTGSFVVNPRATQA"
     gene            complement(601..>1282)
                     /locus_tag="VII37_00530"
     CDS             complement(601..>1282)
                     /locus_tag="VII37_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5068229.1"
                     /translation="VTPQTPPPAISEPQQPRPGLNIVVLDPAHGGTDTGARGTGGIRE
                     SEIVLQFALQVRRALEAQGFQVIQTRMANENPSFDDRSAMANAQIGAVFVSLHVSSTG
                     LPGTVRVYVNSDLPASPGGRQAGGLISWDRAQAPFLLLSHKLGDFVQGNLAVRFRGSP
                     GTAQASAIRQLRTTAAPAIAVEISSVSVNDRVDLDRMAPGVADAIARGVTDFRPSYVV
                     AGAPGGLR"
BASE COUNT          289 a          359 c          365 g          269 t
ORIGIN      
        1 ctgcttgcgt cgctcgaggg ttgaccacaa aagaaccggt aaggtctaga tggcctgcca
       61 gcgtttcaac ttcctgtcca tgaatcaaaa ttttaagccg catgatttgt ggaatgttag
      121 cggccagtgt gcgtgtaatg gaatccaccg ctcgttgttc gctttcaatc cccgatggaa
      181 tggaactggc catcgcttcg gaaaaatccg cgatgccggt tccgtctggc aaaaggtaga
      241 acgccaataa caccgcgtcg ggcggcagcg tacgtaactc gagatcggcg ggaccggcta
      301 acaaagtatt gagcacttgt ttggcgcgca acgcgggatc gcccgacaac ggcaactcga
      361 tggttactgg agtgagtgag ccgtcatgcg cgtttgaagc ccaaaacagt tttgccttta
      421 cgcgcgcttc cgtcggagtg gctgttaatg attgcgtcaa ctctctgcgt gcctgttcct
      481 cagattgcgg ctgaattttg gcggtctgct ttacgcgttg cttcaagatc ggaaagtaca
      541 gtccggcgat aatcaccgct ccaaataatc ctcccagtac caatcgaacc caccgtgcgg
      601 tcaacgtagt cccccggggg cgccggctac cacataagac gggcgaaaat cagtgacgcc
      661 acgagcgatg gcgtcggcta ctcccggagc catgcgatct aaatcaacgc ggtcattcac
      721 ggaaacgctg gaaatttcca cggcaatcgc tggagcggcg gtggttcgta actgacgaat
      781 agcggaagct tgcgcggtgc caggcgagcc cctgaatcgc accgccaagt tgccctgcac
      841 aaaatccccc agcttgtgac ttagaaggag gaacggcgct tgagcgcgat cccaggaaat
      901 tagcccaccc gcttgacgtc cgccggggct agccggcaaa tctgaattca cgtagacacg
      961 tacggtcccc ggcagcccag tagaggacac gtgcaagctc acaaatactg cgccaatttg
     1021 tgcgttggcc attgctgagc ggtcatcaaa cgaagggttc tcattggcca tccgtgtctg
     1081 tatcacttga aaaccttgcg cttcaagagc gcgacgaacc tgaagcgcga attgcagaac
     1141 aatttcactc tcgcggatac caccggtgcc ccgtgcacct gtgtccgtgc cgccgtgcgc
     1201 agggtcgagc acgacgatat tcagtccagg ccggggctgc tggggctcgg atatagcagg
     1261 aggtggagtt tgtggagtaa ct
//
LOCUS       DATCBB010000044         5863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_325885, whole genome shotgun sequence.
ACCESSION   DATCBB010000044 DATCBB010000000
VERSION     DATCBB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325885"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VII37_00535"
     CDS             complement(<1..277)
                     /locus_tag="VII37_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068230.1"
                     /translation="MKRRQFVAGASGLALTGVFAPRHVFGAPSAPLGFSFTDVTTAAG
                     IQFQHNSGAFGGKFLPETLGSGCAFLDYDNDGWQDILLINGRDWPGHK"
     gene            complement(336..965)
                     /locus_tag="VII37_00540"
     CDS             complement(336..965)
                     /locus_tag="VII37_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5068231.1"
                     /translation="MARKITVLLVDDHALVRRGFRRMLEDDAEISVVAEASDGPEAVR
                     LALELRPQVIVMDCALPEFNGIEATRRIRKEFPTVEILMLSMHSEDTLVRQALDAGAR
                     GYVLKNAMDLDLVNAVKAVALGQAVLDPQVSKPASLKGERGSGLTTRELEILQHIVAG
                     KSNKEIAVDLGLSSNTVAVHRANIMDALGIHKTAELVVYAIRNGLVSLP"
     gene            complement(955..2310)
                     /locus_tag="VII37_00545"
     CDS             complement(955..2310)
                     /locus_tag="VII37_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5068232.1"
                     /translation="MQKLSSQRSPTTGLLFGLIITLAAVLAYASYITVQLSGLRRLQS
                     DMVDRNRKDSLQLLRIQNDLNSIALAMRDMLDATEPYPLTAWAAQFERIHADLDAALK
                     LEEQFAGANRTPEQRQYLDQSLAQFWDAMNRMFALASASKENEAREQIRLTLQPRQQA
                     LSTAVSRLLVENNEGEEQAAGRIEHIYDSVQRQLYLFLAATLIAIVLTSLYLIRANRQ
                     IFARLAELSEQRSDLAQKLISTQESTLRHISRELHDEFGQVLTAIGAMLGRAGNQAPE
                     GSPLRADLQEVREIAQSTLNNIRSLSQALHPVLLEEAGLESTLDWYIPTVEHQTGIRL
                     HYEKSGEPFSIDTGAGVHLYRIIQEALNNVSRHAGVTEAWIRLKFLSHALELEVEDHG
                     KGFATGSNPNGNMTRGIGLVGMRERAELIGGALEISHPAEGGTLVRLKIPQDKLQTHG
                     A"
     gene            complement(2334..3524)
                     /locus_tag="VII37_00550"
     CDS             complement(2334..3524)
                     /locus_tag="VII37_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5068233.1"
                     /translation="MTTRMLPASNASEAHHHKLVHAALFAGFVVTGIIATILGPSLPT
                     FIARWTLDDTQAGLFFTTQFAGSLLGVLLSSAILSTRGYRDALVLGFFLMAVGVAGLN
                     SSSHYIALLATALYGFGFGVVIPATNLCMAEISGVRRAAVLNLLNMAWGVGAIGCPAL
                     LLAGLRANHFSAVLLAIAASALLLSILSVGMKFEGLAPKPASGVAAEQKTNRAHPVHV
                     PVALGLLFFLYIGTENGVSGWAAEQAHRIGAGNAAVVMPMFFWAGLLSGRGISALILT
                     RVKESWLVITGLVLSALGTTCLLLASSRSQVIVGVVAAGFGLASVYPIFISWLSKWYG
                     ERARRLGGVMFSVAALGGATMPWTVGFISQRANSLRVGLLVPLCGCVAMIALIAVLRR
                     RIAA"
     gene            3630..4670
                     /locus_tag="VII37_00555"
     CDS             3630..4670
                     /locus_tag="VII37_00555"
                     /inference="COORDINATES: protein motif:HMM:NF008607.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY5068234.1"
                     /translation="MIDVGLIGFGLAGRAFHAQVIRATPGLRLAAILQRQGSEAAQKY
                     PDVRVVRSVEELLGIADIRLVVIATPNETHAPIARQCLEAGRDVVVDKPFATTLKEAA
                     ELVDLARRCGRLITVYQNRRFDGDFQAIRQIVDDGTLGRIIRFETNYDRFRPDLKPGA
                     WRERPGPGTGIFFDIAPHLIDHALVLFGLPEAVTADIRLERAVAVIDDAFDVMLHYPA
                     GMRAVLRSTMLAIATRPRFILHGTHGSFFKQTYDPQEINLRRGYIPETGPWGGEPQKD
                     WGVLTTVENDVVTKRSVPSSTSDYRDYYANVRDAILGRATLAVSPEYALALMRVLELA
                     QESSRKRCTIAW"
     gene            4712..5395
                     /gene="rpe"
                     /locus_tag="VII37_00560"
     CDS             4712..5395
                     /gene="rpe"
                     /locus_tag="VII37_00560"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY5068235.1"
                     /translation="MAKIELVPSILSANFAQLAVDAKAALDAGGTVLHVDVMDGHFVP
                     NITIGPPVVKSLRAAIPDAIFDCHLMIENPDELIPAFAEAGATWISVHQEACVHLDRT
                     LHLIDSHGCQPGVVINPATPVSMLDEVLDIVHHVLVMSVNPGFGGQKFIRGALRKIES
                     LASTRARRGLNFRIEVDGGIAMETVADVVRAGAELLVAGNAVFGSGNIADNVRNLLQA
                     AKAATLTRA"
     gene            complement(5451..>5863)
                     /locus_tag="VII37_00565"
     CDS             complement(5451..>5863)
                     /locus_tag="VII37_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY5068236.1"
                     /translation="VQGFDDHLENVAKVVGVSDDLEAVQRCEKDTQEACGNRASAARS
                     QPYYLDVTHPKANKGEVVLAMSDLLQTPVDQIATIGDMPNDVLMFRKSGISIAMGNAS
                     PEVQKSATYVTASNEQEGFAEAMEKFVLQQRAAT"
BASE COUNT         1362 a         1691 c         1595 g         1215 t
ORIGIN      
        1 gcttgtggcc cggccaatcc ctaccattga tcaacaaaat gtcttgccac ccgtcgttgt
       61 cgtagtccag aaaagcacaa ccagatccca acgtttcggg caaaaatttc cccccgaagg
      121 caccgctatt gtgctgaaac tgaatccccg ccgcagtggt gacatcagtg aaactgaacc
      181 ccagcggcgc tgatggggca ccaaaaacat ggcgaggagc gaacacaccg gtaagcgcta
      241 agccactcgc gccagcaaca aactgccggc gcttcatgaa agctcacaaa acaggaaagc
      301 ccacatcaag gtccacacct catggaaggc gcgcatcacg gaagactgac cagtccgttg
      361 cgaatggcat acaccaccaa ctcggcggtt ttgtgaatcc ctagcgcgtc catgatgttc
      421 gcgcgatgca ccgccacggt gttcgaactg aggcccaaat cgacggcaat ttccttattg
      481 gatttccccg ccacgatgtg ttgcagaatt tccagctcgc gagtggtaag cccggagccg
      541 cgctcgccct taagcgatgc cggcttggaa acctgcggat ccaaaacagc ttggcccaac
      601 gcaactgctt tcacagcatt gacgagatca agatccatcg cattttttaa aacatatccg
      661 cgcgcgcccg cgtccagagc ctggcgcacc agggtgtctt cggagtgcat gctcaacatt
      721 aaaatttcta ccgtcggaaa ttccttgcgg atacgccgcg tcgcctcaat gccattgaat
      781 tcgggaagcg cgcagtccat tacgattacc tgcgggcgca attcgagcgc gaggcggacg
      841 gcttcgggtc catcgctggc ttccgcaacc acggagattt ccgcatcatc ttccaacatc
      901 cgccggaaac cgcggcgcac cagcgcatga tcatccacga gcaaaacggt aattttacgc
      961 gccatgagtt tgcaatttgt cttggggaat ttttaagcga accaatgttc ctccctcagc
     1021 tgggtgcgaa atctccaaag ctccgccgat caattcagcg cgctcgcgca tgcccaccag
     1081 gccgataccc cgagtcatat ttccatttgg gttcgagcca gtggcaaatc ctttgccgtg
     1141 gtcttccact tccaactcca aagcatgcga caaaaatttg agccgaatcc aagcctcggt
     1201 aacgccggcg tggcggctaa cgttattaag agcttcctga ataatgcgat agagatgaac
     1261 cccagctccc gtgtctattg aaaaaggctc tccggatttt tcgtagtgca aacgaatacc
     1321 cgtttggtgc tccaccgtag gaatatacca gtccagcgtg ctctccagcc cagcttcttc
     1381 cagcaaaaca ggatgcagcg cctgtgaaag gctgcgaata ttattcagcg tggattgcgc
     1441 gatttcccgt acttcctgaa gatccgcacg aagtggtgag ccttcgggcg cctgatttcc
     1501 ggcacgtcca agcatcgcgc cgatggccgt caggacctgt ccaaactcgt catgcaattc
     1561 tcgcgaaatg tgccgcagcg tggattcctg cgtggagatt aatttttgcg cgagatcgct
     1621 gcgctgctcg gacagttcag cgagccttgc aaaaatctgc cggttggcgc gaatgagata
     1681 aaggctagtg agcacgatgg cgatgagcgt cgcggcaaga aaaagataca gttgccgttg
     1741 cacgctgtcg taaatgtgct cgatgcgtcc cgcagcttgc tcctcgcctt cgttgttttc
     1801 aaccaacagg cgcgataccg cggtgcttaa agcctgctgg cggggttgca gcgtcaggcg
     1861 gatttgctcc cgcgcctcgt tttctttgga agcactagct aacgcgaaca tccgattcat
     1921 cgcatcccag aattgcgcta gcgactggtc cagatattga cgttgctccg gagtgcgatt
     1981 cgcgccggca aattgttctt cgagtttcag tgcggcatcc agatcggcat gaatgcgttc
     2041 gaactgcgcc gcccacgcag tcaggggata aggctcggta gcgtcaagca tgtcgcgcat
     2101 agccagcgcg atcgaattca aatcattctg aatgcgcaga agctgcagcg aatctttgcg
     2161 attgcgatcg accatatcgc tctgcagccg gcgcaatccg gaaagctgca cggtaatgta
     2221 ggacgcgtaa gccagcaccg ccgcgagcgt aatgatcaaa ccgaaaagca ggccggtagt
     2281 cggcgagcgc tgggaagaga gcttctgcac gggctgcacc gggaaagaat acctcaggcc
     2341 gcgatgcggc ggcgcaacac tgcgatcaac gcgatcatag cgacgcatcc gcaaagcgga
     2401 accagcaagc ctactcgcag actgttggcc cgttgcgaaa taaatcctac cgtccagggc
     2461 atcgtcgccc cgcccagtgc cgccaccgaa aacatcacac caccgagtcg gcgagctcgt
     2521 tcgccatacc atttggaaag ccaggaaatg aaaatcggat aaacgctggc gagcccaaaa
     2581 ccagccgcta ccaccccgac aatcacctgc gagcgcgacg aagcgagaag caggcaggtt
     2641 gttcccaatg ccgacagcac caatccagta atcaccagcc aactttcttt gactctcgtg
     2701 agtatcaacg cagagatgcc gcgcccggac aacagtccag cccagaaaaa catcggcatg
     2761 actacggcag cgtttccggc gccaattctg tgggcttgct cggcggccca accggagacg
     2821 ccattctcgg tgccgatgta caagaaaaag agcagcccta gcgctaccgg aacgtggacg
     2881 ggatgcgccc tgttagtttt ttgctcagct gccacgccgc ttgctggctt tggagccaat
     2941 ccctcgaatt tcatgcccac agataaaatg ctcagaagca gtgcgctggc ggcaatagca
     3001 agcaaaaccg cgctgaagtg attagctcga aggccggcca acagtaaagc cggacagccg
     3061 atggccccaa ctccccaagc catatttaat aaatttagca ctgcagcgcg tcgtaccccg
     3121 gaaatttccg ccatgcacag gtttgtggcc ggaatgacca ccccgaaacc gaagccataa
     3181 agcgcggtcg ctaacaaagc gatgtaatgg ctgctgctat tcagcccggc aactcccacg
     3241 gccataagaa aaaagcccag caccagcgcg tctcgatatc cgcgcgtcga gagaatcgcg
     3301 ctcgagagca atacccccaa cagtgatcct gcgaattgcg tggtaaaaaa taaccccgct
     3361 tgcgtatcgt caagagtcca gcgcgcgata aaagtgggaa ggctcgggcc aagaattgtg
     3421 gctattatgc cggtgaccac gaatcctgcg aatagtgcag cgtggactaa tttatgatgg
     3481 tgagcttcgc tggcgttgga ggcgggcaac atgcgcgttg tcattttgcg cgaggtgaac
     3541 gccgcaggca atgagacagt agccttcgca ggctaacgat gacatggatt gactttaatg
     3601 aattcaaatt cgtctcagga tgaaaagtaa tgattgatgt cggtttgatc ggatttggtt
     3661 tagcgggccg cgcatttcat gctcaagtaa ttcgcgcaac gccggggttg cgtttggctg
     3721 ccattctgca acgtcaagga agcgaagcag cgcaaaagta ccctgacgtg cgagtggtgc
     3781 gcagtgttga ggaactttta ggtattgccg acattcgact ggtggtcatc gccacgccga
     3841 atgagacaca tgcgcccatt gcacggcaat gcctggaagc cggacgcgat gtagtcgttg
     3901 acaagccatt cgccaccact ctgaaagaag ccgcggaact cgttgacttg gcgcgaagat
     3961 gcggccgatt gatcaccgtc tatcaaaacc gtcgattcga cggagatttt caagccatac
     4021 gccagatcgt cgacgacggc acgctggggc gaattatccg cttcgaaaca aattatgatc
     4081 gttttcgacc tgacctaaag ccgggagcgt ggcgcgaacg gcccggtcca ggcactggaa
     4141 ttttcttcga catcgcgcca cacctgatcg atcacgcgct ggtgctcttc ggtctgccgg
     4201 aagcggtcac agcggacatt cgtctggaac gcgcagtcgc ggtgatcgat gatgctttcg
     4261 acgtgatgct ccactatcct gcgggtatgc gcgcagtgct gcgttcgacc atgctcgcga
     4321 ttgcaacgcg cccaaggttt atattgcatg gcacgcacgg atcatttttc aagcagacct
     4381 acgatcccca agaaataaat ttgcgccgtg gatatatccc tgaaactggc ccctggggtg
     4441 gcgagcctca gaaagattgg ggcgtcctca ccactgtcga gaacgacgtc gttacaaagc
     4501 gcagtgtgcc atcttccacc agcgactatc gcgattacta cgccaacgtg cgtgacgcga
     4561 ttttaggccg cgcaacgctg gcggtcagtc ccgaatacgc cttggccctg atgcgcgtgt
     4621 tggagctggc ccaggaaagc agccgcaagc gttgcacgat agcttggtag agttgcgtat
     4681 aatcttgtga gcactgccgc aggagcgtgc gttggctaaa atagagttag ttccatcgat
     4741 attgtccgcg aatttcgcgc agctcgctgt tgatgccaaa gctgcgctgg atgctggcgg
     4801 cacggtgttg cacgtcgacg tgatggacgg ccactttgtc ccgaatatca ccatcggccc
     4861 gccggtcgtt aaatcgctgc gcgcggcgat tcccgacgca atcttcgact gccacctgat
     4921 gattgaaaat ccggatgaac tgattccggc cttcgcggaa gcgggcgcca cttggatctc
     4981 cgtgcaccag gaagcttgcg tgcatctgga tcgcaccttg catctcatcg actcgcacgg
     5041 ctgccagcct ggcgtggtga tcaaccccgc cacaccggtg tcgatgctgg atgaggtgct
     5101 ggacatcgtt caccacgtgc tggtgatgtc ggtgaatcca ggtttcggcg gacagaaatt
     5161 cattcgcgga gcgctccgca agatagagtc actggcaagc acgagggcaa ggcgcggcct
     5221 gaatttcaga atcgaagttg acggcggcat cgcgatggaa acggtggcgg acgtggtgcg
     5281 ggccggagct gaactgctgg tagccgggaa cgccgttttt ggaagcggga acatcgccga
     5341 caatgtcaga aatcttctgc aggccgccaa agccgcgaca ctcactcgtg cttaggggtg
     5401 ggactgtact tctccaatca cagaaaacta tgcggaacta actagcagaa ctaggtagca
     5461 gcacgttgtt gcagtacgaa tttctccatc gcctcggcaa agccttcctg ttcattcgac
     5521 gccgtaacgt acgtcgcgga tttctgcacc tccgggctgg cattgcccat ggcaatgctg
     5581 atgccgcttt tgcgaaacat caacacatcg ttgggcatgt cgccgatcgt agcgatctga
     5641 tccacgggcg tctgcaaaag atccgacatt gccagcacca cttcgccttt attagctttg
     5701 ggatgcgtca cgtcgagata gtacggctgc gagcgcgccg ccgaggctcg atttccgcag
     5761 gcttcctgcg tgtctttctc gcagcgttgc acggcctcaa ggtcgtcgct aacgcccaca
     5821 actttcgcaa cattttccaa atggtcgtca aagccttgca cca
//
LOCUS       DATCBB010000045         1015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_455853, whole genome shotgun sequence.
ACCESSION   DATCBB010000045 DATCBB010000000
VERSION     DATCBB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455853"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VII37_00570"
     CDS             complement(<1..712)
                     /locus_tag="VII37_00570"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5068237.1"
                     /translation="MTTLQGRYEFIETLGTGATSRVDKARDTLIGRTVALKTFLHGFG
                     SSDVQKQFLREAQIVGRLAHPYIVGLHDVGTDSNGVPYFVMEYVEGKTLERFLDAGPL
                     PLEKAVMWTADLAAALARAHRAKVIHGDVKPANILVTKDGQVKLGDFGIARFATQVST
                     SGSLMGTPAYLSPEQIQGHTQGTRSDLFSLGIILYQMTTGVRPFNGSSVSAVCAQIVS
                     ITPPPPSYHNPSLPAAFDR"
BASE COUNT          208 a          311 c          279 g          217 t
ORIGIN      
        1 cgcgatcgaa cgccgccggc aagctcggat tgtgatacga aggcggaggc ggcgtgatcg
       61 aaacgatttg cgcgcacacc gcgctgactg agcttccgtt gaacggccgc acgccggtgg
      121 tcatctgata cagaatgatg cccagcgaaa ataaatccga gcgcgtgccc tgcgtatgtc
      181 cctgaatctg ctccggagac aggtaagccg gcgtgcccat cagacttccg gaagtagaca
      241 cctgcgttgc aaatctcgcg attccaaaat cacccagctt cacctgacca tccttggtta
      301 ccaggatgtt cgccggtttg acgtccccgt gaatcacctt ggcccggtgc gcccgcgcca
      361 gcgctgcggc gagatctgcc gtccacatta ccgccttttc cagcggcagc ggacccgcgt
      421 ccagaaacct ctccagcgtc tttccctcca cgtattccat cacgaaatac ggcacgccat
      481 tgctgtccgt gcccacgtcg tggagcccca cgatataggg atgcgccagc cgcccgacga
      541 tctgggcttc gcgcaggaat tgcttttgca cgtcgctgga tccgaatcca tggagaaatg
      601 ttttcagcgc gacggtacgg ccaatcagcg tgtcacgagc tttgtccacg cggctggtgg
      661 cgccggtacc taacgtttca ataaattcgt agcgaccctg cagagttgtc attattcttt
      721 cgccaggcga gcgagcgtta cgcctttgct ataccaacat tgtcaatctg cggtttgcgg
      781 ctggaaatga cccgatggtc ctgtttttga aggactcttg tacctgtccc tgcgcactaa
      841 gtggattccc gtagcggaaa gatggagaag aaaaattatc tcggtattac gccgaacgca
      901 gcagcttggc cgcgcgcgag cccaggctta acggctgtac gatcacctgc gtggtcacct
      961 tgcgtcgcgc gaagggatac agatcgttcg ccagcgtctc cgccgacgaa taacg
//
LOCUS       DATCBB010000046         1284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_196256, whole genome shotgun sequence.
ACCESSION   DATCBB010000046 DATCBB010000000
VERSION     DATCBB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196256"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VII37_00575"
     CDS             <1..1136
                     /locus_tag="VII37_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5068238.1"
                     /translation="WRTFFALFFLLSAFLTQSNLTNAENTRFDLAQLKAPTGFHISVF
                     AQEIDGPRMMVFTPGGVLLVSESGEGRVKALPDPKHSGKSAQPVTVLEGLNEPHGLAF
                     YDGKLYVAENDKLRRYDWDETKLRASNPKKLTDLPGGGGHSTRTVVFHGGKMYVSAGS
                     SCNACVEKDQRRGAVMEFNPDGTGQKIFAKGLRNAVGLAVNPKTDTVWATVNGRDMSG
                     DDLPPEVIVDLGKNGGDFGWPYCYGDHVPDPNFTKAGDNRCQNVIGPKVQMQAHSAPL
                     GLAFYERSEFPAEYQNSIFVAFHGSWNRSIPTGYKIVRVKLDDHGQPQTGAEDFITGW
                     LAPGEKKHGRWMGRPVGIVFGGDGAMYVSDDAAGVIYRVTYGK"
BASE COUNT          276 a          322 c          387 g          299 t
ORIGIN      
        1 gttggcgcac gttttttgct ctgtttttcc ttctatcggc atttttaacg cagtccaact
       61 tgacgaatgc cgaaaacacc cgcttcgatc tcgcgcaact gaaggcgcct accggattcc
      121 acatctcagt cttcgcccag gaaattgacg gcccgcgcat gatggttttc actcccggcg
      181 gagttctgct ggtgagcgaa tcgggggaag gcagggtgaa agcgcttccc gaccctaagc
      241 actccggtaa atcagctcag ccggtaacgg tgctggaagg gctgaatgag ccgcatgggc
      301 tcgcgtttta tgacggcaag ctctacgttg cggaaaatga caaactgcga cgttatgact
      361 gggatgagac aaaattgcgg gcgtcgaatc cgaaaaaact cacagatctg cccggcggcg
      421 gcggacattc gacccgcacc gtcgtctttc atggcggcaa gatgtacgtc tcagccggtt
      481 cgagctgcaa tgcctgcgtt gaaaaagatc agcgacgcgg ggctgtgatg gaatttaatc
      541 cggacggcac cggccagaaa atcttcgcca aaggcctgcg taatgccgtc gggctggcgg
      601 tcaatcccaa gacagatacg gtgtgggcca ctgtgaatgg ccgcgatatg tcgggtgacg
      661 atttgccgcc cgaagtaatc gttgatctgg gtaagaacgg cggtgatttt ggttggccct
      721 attgttacgg cgatcatgtt ccagatccaa atttcacgaa ggctggtgac aatcgctgcc
      781 agaacgtgat cggcccgaag gtgcagatgc aggcgcactc cgctccgctg gggcttgcgt
      841 tttatgagcg ctcggaattt cccgccgaat atcagaacag catcttcgtc gcattccatg
      901 gatcgtggaa tcgcagcatt cccaccggat acaaaatcgt ccgcgtgaag ctcgatgacc
      961 atggccagcc gcagactggt gctgaagatt tcattacggg atggcttgcc cccggcgaaa
     1021 aaaagcatgg ccgttggatg gggcggccgg tagggattgt gttcggtggt gatggcgcga
     1081 tgtatgttag cgatgatgct gcgggcgtca tttaccgggt gacatatggg aagtagttga
     1141 gttatgaggt ggaggcgcat cttgcttgtc cggcagttgg gtggccgggc ggggacgccc
     1201 ggcgctccat tattcaatta ttcaatttct tcattagctg cagcaatatt tctcgtactg
     1261 ctggtaaata aacgggtcgg tcat
//
LOCUS       DATCBB010000047         1562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_325946, whole genome shotgun sequence.
ACCESSION   DATCBB010000047 DATCBB010000000
VERSION     DATCBB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325946"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VII37_00580"
     CDS             complement(<1..695)
                     /locus_tag="VII37_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5068239.1"
                     /translation="MALHDKFGRQITDLRISVTDRCNFRCVYCRSADPENYRDHDELL
                     SWAELSRLARIFKTLGIRKMRVTGGEPLVRPGVEEFISHLRDLGVEDISMTTNGHLLS
                     ERSARLVSAGLQRINISLDSLDAAKFERITRTKSFASVMKGIDAVQASGLAPAKINAV
                     LVRGINDDEVEDFASFARERGVVMRFIEFMPLDADHHWKRSSVVPAAEVYGRIHARWP
                     LEEIPHHYSETAR"
     regulatory      complement(764..936)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            993..>1562
                     /locus_tag="VII37_00585"
     CDS             993..>1562
                     /locus_tag="VII37_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY5068240.1"
                     /translation="MRIFVLGAGATGSLLAQLLERQGHDVWCGDRDLARARRFLGKKS
                     TIPVTEVNARNLLGIVRAARGCQLIVNASASVCNQIVMRAALRLRAHYLDLSSHLTRN
                     PFKSEQFAFAKRFEAKNRIALINTGAAPGLTNLLVKRAAEALHEVESVQIRLYESTES
                     HDPISQWSPEVSFDEAISNPRVYRNGKFLM"
BASE COUNT          357 a          482 c          392 g          331 t
ORIGIN      
        1 tttcgcgccg tctcgctgta atggtgagga atttcttcca gcggccagcg cgcgtgaatg
       61 cggccgtaaa cttctgcagc aggcaccacc gagctgcgct tccagtgatg atcggcatct
      121 agcggcataa attcaatgaa acgcatcacc acgcctcgct cgcgcgcgaa gctcgcaaaa
      181 tcttccacct cgtcgtcgtt aatccctcgc acgagaacag catttatttt tgccggcgcc
      241 agtcccgacg cctgcaccgc atcgataccc ttcatcaccg aggcaaacga tttggtgcgc
      301 gtgatgcgct cgaatttcgc ggcgtccagc gaatccaggc tgatattgat gcgctgcaag
      361 cccgccgaaa ccagccgcgc gctgcgttcc gagagcaaat gaccgttagt ggtcatggaa
      421 atatcttcga cacccaggtc tcgaagatga gaaatgaact cttccactcc cggtcgcacc
      481 aacggctcgc caccggtaac acgcattttg cgaatgccta gtgtcttgaa aatccgcgca
      541 aggcgagaaa gctccgccca cgacaaaagt tcatcatggt cgcgatagtt ttccggatcc
      601 gccgagcggc agtacacgca acgaaaattg caacgatccg tgaccgagat acggagatcg
      661 gtgatttgcc gtccgaattt gtcgtgaagc gccacgttta tccaagcgac ctcaaaaaac
      721 aaaaagcccg atccagtaat ggaatcgggc gccatccgaa atcaggtttc ggagaagtgc
      781 tcctttccaa attaccggga ggcccgagcg tttccacccg aaccctgagc gatcagctcg
      841 cccaggtcaa tccattcccg ggggctgatc acgtcgtccc tggcgccatc ggttttatct
      901 ccgtttaggc ggcaggaatc ggagttacag cactagtcta atcctctccc gggcacatcg
      961 caattcgggt gtggtagagt tttgcccaca ccatgcgcat cttcgttttg ggagccggag
     1021 ccaccggctc actccttgca cagctgctcg agcgtcaggg acatgacgtt tggtgtggtg
     1081 accgcgatct cgctcgcgcg cgcagattcc tcggcaagaa aagcaccatt cctgttactg
     1141 aagtgaacgc ccgtaattta ctgggaatag tccgtgcagc ccgcggctgc cagctcatcg
     1201 tcaatgcttc agcatccgtt tgtaaccaga tcgtcatgcg agccgcgctg cgcttgcgcg
     1261 cgcactacct cgatttaagc tcacacctaa cgcgcaatcc cttcaagtcc gaacaattcg
     1321 cattcgccaa acgctttgaa gccaaaaatc gcatcgcgct gatcaacaca ggagccgccc
     1381 cagggctcac caatttgctg gtgaaacgcg ccgccgaagc cctccatgaa gtcgaatccg
     1441 tacagattcg cttgtacgaa agcaccgaaa gccatgaccc tatttcgcaa tggtcgccgg
     1501 aggtctcatt tgacgaggcc atctccaacc cgcgcgttta tcgcaatggt aaattcctga
     1561 tg
//
LOCUS       DATCBB010000048         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_455960, whole genome shotgun sequence.
ACCESSION   DATCBB010000048 DATCBB010000000
VERSION     DATCBB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455960"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VII37_00590"
     CDS             complement(<1..366)
                     /locus_tag="VII37_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068241.1"
                     /translation="MAWQGLKLNLRVSQKQILTPGLVQMVSVLALNRLELREMINQEM
                     IANPVLEELSEEPTQTDNYSDENFLKAETEKVPEKPEANPFDEFDVGSFFNQYLDTGG
                     DGGQSQEREVSERPSFEKFL"
     gene            complement(395..1120)
                     /gene="lptB"
                     /locus_tag="VII37_00595"
     CDS             complement(395..1120)
                     /gene="lptB"
                     /locus_tag="VII37_00595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY5068242.1"
                     /translation="MLNLQAVELSKTYRGRKVVDDVQLEIGQGEVVGLLGPNGAGKTT
                     TFYIMVGLTRPDSGRVLLNGEEITDLPMYLRARSGISYLPQEPSVFRQLTSEENLLAV
                     LETLPLTPEQQRDRLEELIVQMGLETVRSSKAYVLSGGERRRLEIARSLTLSPSFILL
                     DEPFSGIDPLTVKDLQETIRDLSRSGIGVLITDHNVRETLNVTDRAYIVNHGKIMAQG
                     TPESLSTDAEVRRVYLGDHFRLN"
     gene            complement(1127..>2790)
                     /locus_tag="VII37_00600"
     CDS             complement(1127..>2790)
                     /locus_tag="VII37_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY5068243.1"
                     /translation="DAGEMKLNLDEDFRARKLLATAGALGKRPHAVSSAASGPMELTA
                     NVLTAWFNADGGLERLDASGKVQGSRKAGAEDEEFTAANSSVELWPEITQPKLINMDG
                     GVILKTSATTGQARILETNKARMEFTEGSDEKPSQPKRAETLAAGTIQWTDARANGQA
                     SNGADAAKDIATRTKLSAEKFVLDFAGGKATQLAASGNVQAQRDIAGAPPQITTAKRG
                     VAHLLANGGWSQIELNENVKFKEGERSGQADHAVAESASQITTLTGQAIVRDASTETR
                     AANIVFMQASGDIRAEGGVRSRTVSVRAATSQVSGTTPQTSGAIPQFSNGPANITSDK
                     MDANAKSGRALYSGHARLWQGDSVLEADSIELLQKSKQLNAVGKVRSVFPQAAGHDAS
                     NSSSTSKSLKKPSLWHIASDTLTYLDLENRAHLSGNVVVQSEVQRMRSDSLDLFFTYA
                     NTAGTAEKGSNGVAGATGAQQISRAVGTGRVVVDEQTRKATAERAEYTATDGKFVMSG
                     GNPTLFDGTQGTTTGRQLTFFLADDTIIVDSEKGSRTLTKHQVQK"
BASE COUNT          544 a          860 c          677 g          709 t
ORIGIN      
        1 caaaaatttt tcaaaggaag gacgctcgga aacttcgcgc tcctgcgact gcccgccatc
       61 gccgccggtg tccagatact ggttgaaaaa actgccgaca tcgaattcat cgaacggatt
      121 ggcttcgggt ttttcgggga ctttttcggt ttcggctttc aggaaatttt cgtcgctata
      181 gttgtcagtt tgggtgggct cttcgcttaa ttcttccaga acggggttgg caatcatttc
      241 ctggttgatc atttcgcgca gttccaggcg gttcagcgcc agaacgctga ccatctgcac
      301 caaaccgggc gtcagaatct gcttctgcga caccctgagg tttagtttta gtccttgcca
      361 cgccattccg tcttgatcct ctgtttctgg cccgttaatt cagtcgaaaa tgatcgccca
      421 gataaacccg ccgaacttcc gcatcggtgc tcaaactctc gggtgtgccc tgcgccatta
      481 ttttcccatg attcacgatg tacgcgcgat ccgttacgtt cagtgtttcc ctcacgttat
      541 gatccgtaat caaaacgcca atccccgacc ggctcaggtc gcgaatcgtt tcctgcaaat
      601 ctttcactgt cagcgggtcg atccccgaga acggttcatc cagcaaaata aatgaaggcg
      661 acagtgtaag cgatcgcgcg atttccagcc gtcggcgttc gccaccggac agcacataag
      721 ctttgctgct tcgcaccgtc tccagcccca tctgcacgat cagttcttcc agccggtcgc
      781 gctgttgctc gggtgtaagc ggcagcgtct caagcaccgc cagaagattt tcttcactgg
      841 taagttgtcg aaaaacggaa ggttcctgag ggaggtagct aattccgctg cgggcccgca
      901 gatacatggg taaatctgta atttcttcgc cgttcaacaa cacacgcccg gaatcgggcc
      961 gcgtcagccc taccattata taaaatgtag tggttttccc tgcaccattg gggccgagca
     1021 gccccacaac ttcgccttgg ccgatctcca gctgcacatc gtcgacgacc ttgcggccgc
     1081 ggtacgtctt acttagctca acagcctgga gattcagcat acctcttcat ttctggactt
     1141 gatgcttcgt caatgttcgc gagccttttt ctgaatcgac gatgatcgta tcatccgcta
     1201 aaaagaaggt caattgacgc cccgtggtgg tgccttgcgt gccgtcaaaa agcgtcggat
     1261 ttcccccgct catcacaaat tttccgtccg tggcggtata ctccgcgcgc tcggcagtcg
     1321 ctttgcgcgt ctgctcatcc acgacaactc tgccggtacc caccgcccgg ctgatttgtt
     1381 gcgcaccagt agcaccggcc acgccattcg aacccttttc cgcagtgccc gcggtattcg
     1441 cgtacgtaaa aaacaaatca agcgagtcac ttcgcatgcg ctgcacctcc gattgcacca
     1501 cgacgttccc cgacaggtgc gcacggtttt ccaaatccag ataggtcaac gtgtccgaag
     1561 cgatgtgcca taagctgggt ttcttcaagg atttgctggt cgaagaacta ttgctcgcgt
     1621 catgcccagc cgcctgcgga aacaccgagc gaaccttgcc cacagcgttc agttgtttgc
     1681 tcttctgcag cagttcgatg gaatcggcct ccaatacaga atcaccttgc caaagtcgcg
     1741 catgcccgct gtaaagtgca cggccactct tggcattcgc atccattttg tcggaagtga
     1801 tattcgcggg tccgttggaa aattgcggta tcgctccgga agtttgcggt gttgtccctg
     1861 aaacttggga agtcgctgcg cgcaccgaaa ctgttctgga gcgcaccccg ccctctgcgc
     1921 gaatatcgcc actagcctgc ataaaaacaa tgttggctgc gcgggtttcc gtcgaagcat
     1981 ctcgcacaat cgcttggccg gtcagcgtcg tgatctggct ggcgctttcc gccacagcat
     2041 gatctgcctg cccgcttcgc tccccttctt tgaactttac attttcgttc agctcgattt
     2101 gcgaccaccc gccgttggcc agtaaatgcg ctacaccacg cttcgccgta gtgatctgcg
     2161 gtggagcacc agcaatgtca cgctgcgctt gtacatttcc actcgccgcc agctgggttg
     2221 ctttaccccc cgcaaaatcg agcacaaatt tctcggcact taacttcgtc cgcgtcgcta
     2281 tatctttagc ggcatccgcc ccattcgagg cctgtccatt cgctcgcgca tccgtccatt
     2341 gaatggtgcc ggcggccaac gtctcggctc tctttggctg gctgggcttt tcgtcgctgc
     2401 cctccgtaaa ttccatacgc gccttattgg tttccagtat tcgtgcttgc ccggtcgtgg
     2461 cgctcgtttt aagtattacg ccgccatcca tatttattaa tttcggctgg gtaatttccg
     2521 gccacagttc cacggaagaa ttggcggccg tgaattcttc atcttcggcg ccggcttttc
     2581 gtgaaccctg aaccttgcca gaggcatcga gtctctcgag tcctccgtcc gcgttgaacc
     2641 acgccgtcaa cacattggcg gtcaactcca tcggcccgga agccgccgac gataccgcgt
     2701 gtggcctttt accgagcgct ccggccgtcg ctaaaagttt ccgcgcgcga aaatcctcat
     2761 ccaaattcaa cttcatttcc ccggcatcca
//
LOCUS       DATCBB010000049         3785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_99141, whole genome shotgun sequence.
ACCESSION   DATCBB010000049 DATCBB010000000
VERSION     DATCBB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99141"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1052)
                     /locus_tag="VII37_00605"
     CDS             complement(42..1052)
                     /locus_tag="VII37_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068244.1"
                     /translation="MALTFFPKPAQADEIRLKDGQKLYGVIVAYEDNMFKVKTDFGFV
                     LVEKDKIASIVPNVTSSHNAAKSEPPAAEKPVAAKPEAASTKPAASAAQKETRPSSRA
                     NPVTIAQPEPVASVSEPLPEKKTISFTGASTPIVPPARSTSGAASIANAPALRTTTKP
                     PSAVLVTASQPAKPKEIVPPGNRETLQGNMYINYTHGFQMYKPPSWKLLDDANKEIPN
                     AIVAMGTANESTLMVVGSEKTKGSLDVTAADVEKRLRETYGNYRRISQRKTVAGGFPA
                     VEIHYRGLADEHDWSGTLLVVSRGDDTFSVLGMTYSDSDLIQIQENVIGRAISSLEFN
                     KN"
     gene            1331..1648
                     /locus_tag="VII37_00610"
     CDS             1331..1648
                     /locus_tag="VII37_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5068245.1"
                     /translation="MSETKETKTFDTVDHIAVAVSDVRGTVDWYLKNFKCQVTYQDDS
                     WALLEFANIRVAFVLPQQHPPHFALLRDPALFGEPKTHRDGTRSVYLHDPSGNSVEVL
                     ALK"
     gene            complement(1713..2636)
                     /locus_tag="VII37_00615"
     CDS             complement(1713..2636)
                     /locus_tag="VII37_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5068246.1"
                     /translation="MTAPRSATNPWPYLALAAGVLCIAWSAIFVRWTDMPGPASAFYR
                     MLIPAVLLIPTWFIGPNSPRVSPKTLMVIAVGGGFFALDLAFFNTSILQTSAANATLL
                     GNNTPVFVGLLSWLIFRQRPSRDFWLGLMLAIAGCTVIVSGDLAKHLKLGWGDAMALA
                     ASACFAVYMLATERVRITTSTLVFLRLAMISSAIFLFIINLALGISLQVPAGRSWAAL
                     FGLGLVSQLGGYLALTYALGHLPATAASISLLSQGPLTAVLAAIILLEPLSTPQIIGG
                     ALVLAGVGLANRRIRPTGEVPANLLETSQHK"
     gene            2805..>3785
                     /locus_tag="VII37_00620"
     CDS             2805..>3785
                     /locus_tag="VII37_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5068247.1"
                     /translation="MHKITRRDFLNGVSVGVGGVLVGASGISTMFGAETLLAAGALDE
                     LSAQDATGYYPPAKMGMRGNHDGSFTFAHLMRDGQPWNELGRAAAIEENYDLVVVGAG
                     ISGLAAAYFYRQRFGKNARILILDNHDDFGGHAKRNEFHAGGRTVLSYGGTQSIESPG
                     KYSDVAKALIRDIGVRVEKLEQAYDQKLYSKMGTAAFFDKGTFGEDRLVTGMNATPWP
                     EFLAKAPLSEELRRDIARVYTEKKDYLAGMSVEEKAVLLTKISYSEYLTKYCKLTPKA
                     LPFFQTFTHDLFCIGIEAVAAMECYKAGDDYNSFTYAGFDGLGFPDSEKEE"
BASE COUNT          856 a          874 c         1191 g          864 t
ORIGIN      
        1 cggcgtgaca ttcctgcggc gtcacaactt ttataccacg tttaattttt gttgaactcc
       61 aggctggaga tggcgcgacc aattacgttt tcctggatct ggatcaagtc gctgtccgag
      121 taagtcattc ccaacacaga aaaggtgtcg tcgcctcggg agaccactaa taaagttccg
      181 gaccagtcat gctcatctgc cagcccgcgg taatgaatct ccacggcagg aaagccgccg
      241 gcgacggttt ttcgctgaga aatgcggcga taattcccgt aggtttcgcg caagcgtttt
      301 tctacatcag cagcagtcac atccagcgag cctttggtct tctcgctacc cacgaccatc
      361 agggttgatt cgtttgcggt gcccatggcg acgatggcat tgggaatttc cttgtttgcg
      421 tcgtcgagca atttccagct tgggggctta tacatctgga atccgtgcgt gtaattgatg
      481 tacatgttgc cttgtagagt ctcgcggtta ccgggaggca ctatttcttt tggcttggcg
      541 ggttggctcg cggttacaag tacggcggaa ggtggcttgg ttgtagttct gagagccggc
      601 gcgttcgcga tggatgcagc gccgctcgtt gatcttgccg gcggaacaat tggcgtggag
      661 gctccagtga aggaaatggt ttttttctcg gggagcggtt cggacacgga agcgacgggc
      721 tctggttgtg cgatggtcac tggatttgcg cgcgatgaag gccgagtctc cttttgcgct
      781 gcgctggctg cgggttttgt tgaagcggct tccggtttgg ctgctacggg cttctccgca
      841 gcgggaggct ctgattttgc agcattgtga ctggacgtga cattagggac gatggaagca
      901 atcttgtctt tttcaaccag cacaaatccg aagtcggtct tcactttgaa catgttgtct
      961 tcgtaggcca cgatcacgcc ataaagcttt tggccatctt ttaggcgaat ctcatccgct
     1021 tgcgcaggct tggggaagaa agtgagcgcc aagacagcgc tgcccaggaa taatgagctg
     1081 cgtgggacgg ccatgtctct gagtatgcta ccgaccgcaa agtggtggca accactgagc
     1141 gaaatggcgt tgtctgtgag gttacatgcg gcgaggggac ggccatcagc tcaggagggt
     1201 aggtcgcggg tggacgaatt tgcgtccgtt tatgtcacgt ggagcttggg gtaagcttat
     1261 ttgtgaatcg gcaaccttga ttgtgatgaa gcaggaacag ggtttcagcc aacgaaatcg
     1321 agaggcatta atgtccgaga ctaaggaaac gaaaacattt gacactgtgg accacattgc
     1381 cgtggcggtc agcgacgtga gagggacggt cgactggtat cttaaaaatt ttaaatgcca
     1441 ggttacctat caggacgata gctgggcgtt gctggaattc gccaatatac gtgtcgcgtt
     1501 tgtgctgccg cagcagcacc cgccgcattt tgcgttgctg cgggacccgg cgttgtttgg
     1561 cgagccgaag acgcaccgcg atgggacgcg atctgtttat ctgcacgatc cgtctggaaa
     1621 ttctgtcgaa gtgctggcgc tgaaataaat gcgctgaagt ttacgttagc ttaattccgc
     1681 agataggcgg ttgtgcggcc gtgtttcccg atttatttgt gctgcgaggt ttctagcagg
     1741 ttggccggga cttcgcctgt ggggcgaatg cggcgattgg ctagaccaac gcccgcgagt
     1801 accagtgcgc cgccgatgat ctgcggcgtg gatagcggct ctaacagaat gatcgcggcc
     1861 agaacagcgg tgagtggccc ctgcgacaat aaactgatcg aggctgctgt ggcgggcaga
     1921 tggccgagcg cgtaagttag cgcaaggtag ccgccaagtt gcgacaccaa gccaaggccg
     1981 aagagcgcgg cccaggaacg gccggcgggc acctgcaacg aaataccaag cgcaaggttg
     2041 atgatgaaaa gaaaaatcgc gctggaaatc atcgccaggc gcagaaaaac aagcgtgctg
     2101 gtggtgatac gcactcgttc ggtggcgagc atatacaccg cgaagcacgc ggatgctgcc
     2161 aaggccatag cgtcgcccca gcccaacttg agatgtttgg cgaggtcgcc ggaaacaatc
     2221 accgtgcaac cggcgatggc cagcattagt cctagccaaa aatcgcgact ggggcgttgg
     2281 cggaagatca gccaactgag cagtcctacg aagacgggag tgttgttgcc gagtaacgtg
     2341 gcattagcgg ctgacgtttg caggatagaa gtgttgaaaa atgcgagatc gagagcgaag
     2401 aaccctcctc cgacggcgat gaccatcagt gttttagggc ttacgcgcgg tgaattgggg
     2461 ccgatgaacc acgtgggaat aagtaaaacc gcgggaatga gcatgcgata gaatgcggac
     2521 gccggaccgg gcatgtctgt ccagcgcaca aagattgctg accacgcgat acacaaaacg
     2581 ccagctgcca gcgcgaggta aggccatgga tttgttgcgc tgcgcggagc ggtcatgaac
     2641 ggagtgagcg cgggttagag ttgcgtggag ttctcaatta acggagtatc gcacacgaag
     2701 gtataggaat tggcggtttt ggattcggat gagatgacat gttgagacaa tttggatgta
     2761 ttcgcgagcc tttgtggata cgggtcagga cgtaatcgaa agatatgcat aagataacgc
     2821 gtcgcgattt tttgaatggc gttagcgtcg gtgtcggcgg ggtgttagtt ggcgccagcg
     2881 gcatttccac gatgtttggc gccgagactt tgttggctgc cggcgcattg gatgaattgt
     2941 cagcgcaaga cgcgactggg tattatccgc ctgcgaaaat gggcatgcgc gggaatcacg
     3001 acggaagctt tacgtttgcg cacctcatgc gcgacgggca accctggaat gagctcggaa
     3061 gagcggcggc gatcgaagaa aactacgatc tggtagtggt cggagcggga atcagtgggc
     3121 tcgcagcggc atatttttat cgccagcgat ttgggaagaa cgcgcgcatt ttaatcttgg
     3181 acaaccacga tgattttggc gggcacgcta aacgaaatga atttcatgcc ggtggtcgca
     3241 cggtacttag ttacggcgga acgcagtcga ttgagagtcc tggaaaatat agcgatgtcg
     3301 cgaaagcatt gattcgagac atcggcgtgc gcgttgaaaa attagaacaa gcgtacgacc
     3361 agaaactgta ttcgaagatg gggaccgcgg cgttttttga caaggggact tttggcgaag
     3421 atcggctggt caccggaatg aatgccacgc cgtggccgga atttctggcc aaggctccgc
     3481 tgtccgaaga attgcgccgc gacatcgcgc gggtgtacac ggagaaaaaa gattatctgg
     3541 cggggatgtc ggtggaagag aaggccgtgc tactgacaaa aatcagctat tcggaatatc
     3601 tgacgaaata ttgcaagctg acgcccaagg cgttgccgtt ttttcagacc ttcacgcatg
     3661 acctgttttg cattgggatt gaagcggtgg cggcgatgga gtgttataag gccggcgatg
     3721 actacaactc cttcacgtat gcgggattcg atgggcttgg ttttcctgat tctgaaaaag
     3781 aggag
//
LOCUS       DATCBB010000050         1349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_326062, whole genome shotgun sequence.
ACCESSION   DATCBB010000050 DATCBB010000000
VERSION     DATCBB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326062"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /gene="ligA"
                     /locus_tag="VII37_00625"
     CDS             complement(<1..1339)
                     /gene="ligA"
                     /locus_tag="VII37_00625"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY5068248.1"
                     /translation="MTRGDGRTGEDVTPNVKTIRSIPLRIDPAALKKVKLLPNFEVRG
                     EIVMPKKAFAELNRQQELSGGKIFANPRNAAAGGVRVLDASITASRRLEFFSYYLLVD
                     GRSPFTKLSESLQALKEMRFRASSDWKLCDGIEDVLAYCDHWEDKREKLPYEIDGVVV
                     KVNSIPIQNELGYTAKAPRWAIAYKYPARQETTVVKDIQVNVGRTGTLTPVAFLEPVQ
                     VGGVTVSRSTLHNMDEIERLGVQIGDTVLVERAGEVIPHVLKVVKEAPDRKPFRMPEK
                     CPECGSTIHKAPDTVAYRCVNAACPAKRRESLLHFAGRHAMNIDGLGEKIVDQLIEQG
                     LVRDVADLYSLKLDKLVALERFAEKSAQNLLDEIEASKKNSLARLIYALGISFVGERT
                     GELLAGHFSSVEDLASASQEQLEEVAEVGPKVAAAIAEFFSEPANRRLIKKLHK"
BASE COUNT          270 a          379 c          371 g          329 t
ORIGIN      
        1 ccttgtgcag ctttttgatg agccggcgat tggcgggctc ggagaaaaat tcggcgatag
       61 cggcggcaac ttttggtccc acttcggcta cttcttcgag ctgttcctga ctggcggagg
      121 caagatcctc tactgaggaa aaatggccgg cgagtaattc gccggtgcgc tcgcctacaa
      181 aactaatgcc cagcgcgtag attaggcgcg ccaggctgtt ctttttgctg gcttcgattt
      241 cgtccaacaa attttgcgcg gatttttcgg cgaagcgttc cagtgcgacc agcttgtcca
      301 gcttcagcga gtacaaatcg gcaacgtcgc gcactaaccc ttgttcaatg agttgatcga
      361 cgattttttc ccccaagcca tcgatattca tggcgtggcg tccggcaaag tggagcagcg
      421 attcccttct cttcgccggg caggccgcgt tgacgcaacg ataggccacc gtgtctggag
      481 ctttgtgaat ggtgctgccg cattctgggc acttttccgg catgcggaac ggcttgcgat
      541 ccggggcttc tttgaccact tttaaaacgt gcggaattac ttctccggcg cgttccacca
      601 acacggtgtc accaatctgc acgcccagcc gctcaatttc atccatgttg tgcagggtgg
      661 agcggctcac ggtgacgcct ccaacttgca ccggctccaa aaacgccacg ggagtaagcg
      721 ttccggtgcg gcctacgttc acctgaatat ctttgaccac ggtggtttcc tggcgtgcgg
      781 gatatttgta ggcgatggcc cagcggggcg ctttcgcggt gtatcccaat tcgttttgta
      841 tggggatgga attcactttc acgacaacgc catcgatttc gtaggggagc ttctcgcgtt
      901 tgtcttccca gtggtcgcaa taggccagca cgtcttcaat tccatcgcac aatttccagt
      961 ccgacgaagc gcggaatcgc atttccttca acgcctgcaa ggattccgaa agtttcgtga
     1021 acggcgatcg accatcgacc agcaaatagt aagaaaagaa ctccaaccga cgcgacgcgg
     1081 tgatcgaagc atccagcacg cgcactccgc cggcagcggc gttacgcgga ttggcaaaaa
     1141 tcttgccgcc cgaaagctct tgttggcgat tcaattcggc gaaagctttt ttgggcatga
     1201 caatttcgcc gcgcacttcg aaattcggca gtagctttac ttttttgagc gcggccggat
     1261 cgattcgcag aggtatggag cgaattgttt ttacattggg cgtgacgtct tcgccggtgc
     1321 gcccgtcgcc acgcgtcaca ccgcgaacg
//
LOCUS       DATCBB010000051         1236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_1843, whole genome shotgun sequence.
ACCESSION   DATCBB010000051 DATCBB010000000
VERSION     DATCBB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VII37_00630"
     CDS             complement(<1..622)
                     /locus_tag="VII37_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5068249.1"
                     /translation="MDHTSQLTLLPATEMAKHIRHRQISAVELVQAHLAQIERLNSQL
                     NAFIQVRSEDALADAHAADATGERNGKLGGNLGPLHGVPVSIKSSIDVAGMRCEAGTK
                     LRKGYIAAQDAPLVARLREAGAIILGVTNTPDLLMAWETDNLLYGRTNNPWDLSRTPG
                     GSSGGEAAAIASGCSAGGIGSDGGGSIRVPAHFSGICGLKPTPGRIP"
     gene            complement(743..>1236)
                     /locus_tag="VII37_00635"
     CDS             complement(743..>1236)
                     /locus_tag="VII37_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY5068250.1"
                     /translation="RKALDEAGAKTTLISIKPGKLQAMKHDEKSSKYDVGATFDDVDP
                     KDFDAVLLPGGGLNADALRVVSAAQEFVRAIEQAGKPLAFICHAPWLLISAKLVEGRT
                     LTSYHTIQDDVRNAGGAWVDKEVVRDGNWVSSRQPSDIPAFNREMITLFSERAPKKSK
                     SAA"
BASE COUNT          232 a          358 c          341 g          305 t
ORIGIN      
        1 cgggaattcg gccaggtgtc ggtttcaatc cgcaaatccc gctaaagtga gcggggacgc
       61 ggatggagcc tccgccatcg ctgccgatgc ctcccgcgga acatccggaa gcaatagccg
      121 cagcctctcc tccgcttgaa ccacctggcg tgcgcgaaag gtcccacgga ttattcgtgc
      181 gcccataaag aaggttgtcc gtctcccacg ccattagcaa atctggcgtg ttggtgacgc
      241 ctaaaatgat cgctccagcc tctcggagcc ttgcgactaa cggcgcatct tgagccgcaa
      301 tatatccctt gcgcaacttg gttccggcct cgcaccgcat tccggcgaca tcaatcgaac
      361 tcttgatgct taccgggact ccgtgcagcg ggccgaggtt cccgccgagt ttgccattgc
      421 gctctcctgt ggcatcagcg gcatgcgcgt ccgccagggc gtcttcactt ctaacctgaa
      481 tgaacgcgtt tagctgtgag ttcaatcgtt cgatttgcgc tagatgtgcc tggaccagtt
      541 caaccgccga gatttgtctg tgacggatat gtttcgccat ttctgtggcg ggaagaagcg
      601 ttagctgaga agtgtggtcc atgaaataac gtgcgagtct agcacgcgcc gttgcggcac
      661 tgtctcgatt gcgtcggagt cggcgcgaaa aagccgccgc aaagggattg cgagcggcta
      721 atcgttgcat ttcatttcca tctcaggcgg cgctttttga ttttttcggc gcccgctcac
      781 tgaaaagggt gatcatttcg cgattgaatg cgggaatatc ggaaggctgc cggctgctga
      841 cccagttgcc gtcgcgaaca acttccttgt caacccatgc gcctccggca ttgcggacat
      901 cgtcttgaat agtgtgatag ctggtcaagg tgcggccttc taccagcttg gctgaaatca
      961 atagccaggg tgcgtgacag ataaaggcca gcggctttcc ggcctgttca atcgctcgga
     1021 caaattcctg ggccgcgctt accactctga gcgcgtcggc attcagcccg cctcccggca
     1081 acagtacggc gtcgaaatct tttgggtcta cgtcatcgaa tgtcgcgccc acgtcgtact
     1141 tcgacgattt ttcgtcgtgc ttcatggctt ggagttttcc cggttttatt gaaatcagcg
     1201 tggtcttcgc acctgcttcg tccaatgctt ttcttg
//
LOCUS       DATCBB010000052         1414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_294017, whole genome shotgun sequence.
ACCESSION   DATCBB010000052 DATCBB010000000
VERSION     DATCBB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294017"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VII37_00640"
     CDS             complement(<1..425)
                     /locus_tag="VII37_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068251.1"
                     /translation="MEELLMRTWFENLCSLARGPLKMSAGMAALSMLACLSASAQEVQ
                     EAGGEANLNLPNLSQVSFLGIDGHKILLFGLIFCVFGLIFGLAIYSKLKNLPVHKSMR
                     DISELIYETCKTYLITQGKFILLLEIFIAIVIVLYFGVLL"
     gene            878..>1414
                     /locus_tag="VII37_00645"
     CDS             878..>1414
                     /locus_tag="VII37_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5068252.1"
                     /translation="MSTNPSVSSQAGAAVSFGADHATTPPVRSNWSREEVAAIYRTPL
                     PELIFRAQTTHRRFHAPDRVQTCQLISIKTGGCPEDCAYCPQSAHYDADVERQGLLDP
                     QHVISVAREAAERGVTRFCMGAAWRQAPEGKDFDNVLEMVRGVSALGMEVCCTLGMLT
                     DSQATRLKEAGLSAYNHNL"
BASE COUNT          369 a          414 c          351 g          280 t
ORIGIN      
        1 agcagcacgc caaaataaag aacgatcacg atggcaataa aaatctccag cagcaaaatg
       61 aatttgccct gcgtgatcaa ataggttttg caggtttcat aaatcagctc ggaaatatcc
      121 cgcatcgatt tgtgcaccgg cagattcttg agcttgctat aaatggccaa accgaagatc
      181 aacccgaaca cgcagaaaat cagtccaaac aggagaattt tgtgcccgtc aataccgagg
      241 aaggagacct gcgacagatt cggaaggttc aggtttgcct cgccgccagc ctcctgaacc
      301 tcctgagcgc tcgcggatag gcacgcgagc atcgatagcg ccgccatgcc ggcgctcatt
      361 ttcagtggcc cgcgagcgag cgaacacaaa ttttcaaacc aggtgcgcat caacaattcc
      421 tccataaatt accaataatt tcagagccct gggccctgtc taacggattc gctcaacact
      481 tttcagcatg cgtacaacgg caccggcggc gcacccgccg atttcaaaca gggccctgcg
      541 ccaaaattac ggcacggcct aacctgtact gtttttctta gcctgaccat ccccggaacc
      601 cctctcaggg acagcgaaaa ggctttgcgc gggctggcaa caaagcgttg atttataaca
      661 tttagcaacg atagcggtca aaccgtccgt cgatggcagg attggagcga aaccaaccgt
      721 aaccacagat cacgccttta gcaacaccgg acacttggcc cagagaaatg aatcacctgg
      781 aatggcaaga cgctgccgcc tagcgatggt aacatgccat tctaaaattc ggcccacagg
      841 tccgggctag tcatacaatt aaagtttgac ggggattatg agcacaaacc catcggtatc
      901 ttcacaggcc ggcgccgctg tatctttcgg tgccgatcac gccaccactc caccggttcg
      961 cagcaactgg agccgcgaag aagtggccgc gatttatcgc actccactcc ctgaattaat
     1021 ttttcgcgcg caaaccacgc atcgcagatt tcacgctccg gaccgcgtgc agacctgcca
     1081 attaatttcc atcaaaaccg gcgggtgccc ggaagactgc gcgtattgcc cgcaaagcgc
     1141 gcattacgat gccgacgtgg aacgccaggg attgctcgac ccgcaacacg taatctccgt
     1201 ggcgcgcgaa gccgccgaac gcggagtgac acgcttctgt atgggcgcgg cgtggcgcca
     1261 agcccctgaa ggcaaggact tcgataatgt gctcgaaatg gtccgtggcg tttcagctct
     1321 tggcatggaa gtctgctgca ctctgggaat gttgaccgat agccaagcca ccagacttaa
     1381 agaggcgggc ctctcggcat ataaccacaa ttta
//
LOCUS       DATCBB010000053         4185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_326213, whole genome shotgun sequence.
ACCESSION   DATCBB010000053 DATCBB010000000
VERSION     DATCBB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326213"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VII37_00650"
     CDS             <1..220
                     /locus_tag="VII37_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068253.1"
                     /translation="GQAGFGERCKLEDGAIIGGQSGVLGGKIIRSGEAVWGTPARSLE
                     KFKEQFAWLARLPELAARIKNLESDVRK"
     gene            253..945
                     /locus_tag="VII37_00655"
     CDS             253..945
                     /locus_tag="VII37_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068254.1"
                     /translation="MQTLASPQKQNWRLIVVGGHTRSIGKTQLVCDVIRAFPQENWIA
                     GKITQYGHGVCAKNGENCGCAPDEHSYAISWEKSADTGTDSSRFLAAGAQRSFWLRTK
                     QGFLAEGLPLLREALAQLHSTTATEPPTLILESNSVLQFVQPSLYFAVIDPSRDDFKD
                     SARIVLDRADALVLRGESDYGSDSEAPWMKLPRQLLNQTPSVRQSEGEPLPLPLQALI
                     RRTLEAPADVRL"
     gene            1170..3185
                     /gene="shc"
                     /locus_tag="VII37_00660"
     CDS             1170..3185
                     /gene="shc"
                     /locus_tag="VII37_00660"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY5068255.1"
                     /translation="MASSVRMEDEMAVAAAPLPWRQQNWAERLESSIERGANHLLSLQ
                     TEEGYWLGELEADTTLESDYIYYLFVLGKADPERIAKLATYVRRRQLADGGWSIYPGG
                     PSELNATCKAYFALKLAGDPIDSPHMTLARASVHALGGLELTNSYTRFYLALVGAVGW
                     ELVPAIPPELMLLPNWFYINIYEFSSWTRAIVIPMMILSALRPNWQLPERAQVDELFK
                     DPTRKRAAFDWSKQLISWRNFFLALDRGLKFYERLPWKPLRQRALREAKSWVLEHMER
                     SEGLGTIYPAMMNCIFSLVAMGYGPDDPLTCRETREFARFEIEDEETIRLQPCVSPVW
                     DTAIAMVALEEAGVAPDDPALVQAADWILSKQILGPGDWQVKNKDAEPGGWAFEFRND
                     FYPDVDDTAFVLMALQRVKFPDAKRMEAATRRGVQWLLSMQNRDGGWGAFDRDNDRRF
                     LCNIPFADHNAMIDPSTADVTARVLECLGRFGWPADHPAIQKAVKFLLKDQCGDGSWF
                     GRWGVNYVYGTSGVLRALETVSLTAQEYCQRAVAWLKSVQQDDGSFGESLKTYSDVSS
                     KGQGASTPSQTAWGLIGLLAGSNTTDPAITRAVSYLVGNQNEDGSWSEHDFTGTGFPC
                     VFYLKYHLYRNSFPVYALSRYHNQARRADEFCALKFQPNEFRLRSGI"
     gene            3191..4036
                     /locus_tag="VII37_00665"
     CDS             3191..4036
                     /locus_tag="VII37_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5068256.1"
                     /translation="MMRFPPRLLWDLTRVRIAQKLFGTARNPWALRLDLAQLRPNTDA
                     AHTDTSPDGIHSSSEISSRDSQVLSRVRASSAPVVWIGGDAPLQHARIGHLVREIVNC
                     GRTVFVEMDGTLLRRRIHEFRPVPRLYLVLPLNGLENTHDSRARHTGNFRATMEALRT
                     AKLSGFHICIETTIFPGSDRIELIALANFISTLGVDGWIHKRPPAAANDEAVSETLQP
                     ARDLIPSRHWRTFSALLDLISVPAQPGLEKKRSAHPVAQKVGKVPTHETREGHDAHEQ
                     GLRAL"
BASE COUNT          906 a         1316 c         1068 g          895 t
ORIGIN      
        1 cggccaggcc ggtttcggcg aacgctgcaa gttggaagat ggcgcgatca taggtggcca
       61 atcgggagtg ctgggaggca aaatcattcg tagcggcgag gccgtctggg gcacacctgc
      121 ccgttctcta gaaaaattca aggaacagtt tgcgtggctg gcccgtctgc ctgaattagc
      181 agccagaatc aaaaatctcg agagcgatgt gagaaaataa gaactgcatc agtcgacgga
      241 aagcaggccc agttgcaaac actggcgtca ccacaaaaac aaaattggcg cctcatcgtc
      301 gtcggtggcc acacccgcag cattggcaaa acccaattag tctgcgacgt aattcgcgca
      361 tttccgcaag aaaactggat cgcgggcaag atcacccaat acggccacgg cgtatgcgca
      421 aaaaatggcg agaattgtgg ctgtgctccc gacgagcaca gttacgccat aagctgggaa
      481 aaaagcgccg acacgggtac cgatagttca cgcttcctgg ccgcgggcgc acaacgctct
      541 ttctggctca gaaccaagca aggcttcctg gcagaagggc tcccgctctt acgcgaggca
      601 ctagcccagt tgcactccac aaccgccacg gaaccgccta ccttaatcct tgagagcaac
      661 tccgtcctcc aattcgtcca acccagtttg tatttcgcgg ttatagaccc cagccgcgat
      721 gattttaaag attccgcccg catagttctg gaccgcgccg acgcgttggt attgcggggc
      781 gaaagcgatt acggttcgga cagcgaagcg ccctggatga aactcccgcg ccaactcctg
      841 aaccagactc cctcggttcg ccaaagcgaa ggcgagcctt tgcctctccc acttcaagcg
      901 ctgatccgac gcactttgga agcccccgca gacgtcaggt tgtaaccttc ccgcgcccag
      961 tttcatctcc aaatgagaca atatcgttga ccgctggggc taatcccggc gataatctac
     1021 tagtactagt gtaggcgttg taagtaggga agcggtatct cccaatccac agccggttcg
     1081 ggcaacggga atagtagttc cgcttttcaa ggtattgtct aagttgctca tcgttctcgc
     1141 gcgatgagcc aatgggggat caccaactga tggcgtcttc tgtccgcatg gaagatgaaa
     1201 tggctgttgc tgcagcgccg ctaccctggc gccagcagaa ctgggctgaa cgcctcgaaa
     1261 gctccataga gcggggcgcc aatcatctct tatccctgca gactgaggaa ggctactggc
     1321 tcggcgaact cgaagccgac actaccctcg aatccgacta tatctattac ctcttcgtac
     1381 taggaaaagc tgaccccgag cgtatcgcta agcttgccac ctacgttcgc cgccgccaac
     1441 tcgcggacgg gggctggagc atttatcccg gcggcccctc tgaactgaac gctacctgca
     1501 aggcctactt tgctctgaaa ttggccggcg acccgatcga ctcgccgcac atgactctcg
     1561 cgcgagcctc agtgcacgcc ttgggcggcc tggaactaac caattcttat actcgcttct
     1621 acctcgctct cgtcggcgcc gttggctggg aactagtccc cgccattccg cccgagttga
     1681 tgctcctgcc caactggttt tacatcaata tttacgaatt ttcttcctgg acgcgcgcca
     1741 tcgtcattcc catgatgatt ctctccgcct tgcgtccgaa ctggcagctt cccgagcgcg
     1801 ctcaagttga tgaacttttc aaagatccaa cccgcaaaag agccgccttc gactggagca
     1861 agcagcttat ctcctggcgc aattttttcc tggccctcga ccgcggcctg aagttttacg
     1921 aacgcctccc ctggaagcct ctacgccaac gcgccttacg cgaagcaaaa tcctgggtcc
     1981 tcgaacacat ggagcgctcc gaaggattgg gcaccatcta cccggcgatg atgaattgca
     2041 tcttttcgct ggtagccatg ggttacggcc ccgacgatcc tctgacctgc cgcgaaacca
     2101 gggaatttgc ccgcttcgaa attgaagacg aggaaaccat tcgtctgcaa ccctgcgttt
     2161 ctcccgtatg ggataccgct atcgctatgg tcgccctcga ggaagctggc gtagctccgg
     2221 acgatcccgc gctagttcag gctgctgact ggattttgtc caaacaaatt ctcggccccg
     2281 gcgactggca agttaaaaac aaagacgccg agccaggtgg ctgggccttc gaatttcgca
     2341 acgattttta tcccgacgtg gacgacaccg cgttcgtcct gatggctttg cagcgcgtga
     2401 aatttcccga cgccaagcgc atggaagccg caactcgccg cggcgttcaa tggcttttga
     2461 gtatgcagaa tcgcgacggt ggttggggcg ctttcgaccg cgacaatgat cgccgcttcc
     2521 tgtgcaatat tccgtttgcc gaccacaacg ccatgatcga tccctccacc gccgacgtca
     2581 ccgctcgcgt actcgagtgc ctcggccgat ttggctggcc cgcagatcat ccggccatcc
     2641 aaaaagcagt taaattttta ctcaaagatc aatgcggcga tggttcctgg ttcggtcgct
     2701 ggggcgttaa ctatgtctac ggcaccagcg gagttctccg agcgctggaa acagtttcgc
     2761 taaccgcgca ggaatactgc cagcgcgctg tagcgtggct gaagtcggtc caacaggacg
     2821 acggcagctt cggcgaatcg ctcaaaacgt actccgacgt ttcttccaag ggccaaggcg
     2881 ccagcacccc ttcgcaaacc gcttggggct tgatcggttt actcgccgga tcaaacacca
     2941 ccgacccagc catcacgcgc gcggtttcct atttggtcgg caaccaaaac gaagacggtt
     3001 cctggagtga gcacgatttc accggcaccg gttttccttg cgtcttttac ctgaaatatc
     3061 atctctatcg caattctttc ccggtatacg ccttgtcgcg ttaccacaac caggcccgca
     3121 gggccgacga attctgcgcc ttgaagttcc agcccaacga attccggctg cgctccggaa
     3181 tctagcgcgc atgatgcgct ttcctccgcg actcctctgg gacctcacca gggttcgcat
     3241 cgcacaaaaa ctttttggca ctgcgcgtaa tccgtgggcc ttgcgcctcg atttagccca
     3301 attgcgacct aacaccgatg ccgcacacac tgacacttcg ccagacggca tccattcgtc
     3361 ttcggaaatt tcttcgagag attcgcaggt tctgtcgcga gtgcgtgcaa gctccgcgcc
     3421 cgtagtatgg atcggtggcg acgctccgct gcaacacgcg cgcatcggcc acttggtgcg
     3481 cgaaattgtc aattgcggcc gaacggtatt cgtcgaaatg gatggcaccc ttttgcgccg
     3541 tcgcatccac gaattccgcc ccgttccgcg cctttactta gttttgccgc tcaacggcct
     3601 ggaaaacact cacgattccc gcgcccggca caccggaaat tttcgtgcca cgatggaagc
     3661 cctccgcacc gccaaacttt ccggctttca catttgcatc gaaaccacaa ttttccccgg
     3721 ctccgacagg atcgaactca ttgcgttagc aaatttcatt tccactttgg gggtcgatgg
     3781 ctggatccac aaacgccctc cagccgccgc taacgacgaa gctgtcagcg agacgctgca
     3841 gcccgctcgc gatttgatac cgagccgcca ttggcgaacc ttttcagcgc ttctcgattt
     3901 aatttctgta ccagcacaac cagggcttga aaaaaaacgc tccgcccacc cggtagctca
     3961 aaaagttgga aaagtgccca cccacgaaac tcgcgagggt catgacgctc atgagcaagg
     4021 actccgcgct ctatgaccac tctcgtcacc ggtgcagcag gattcctggg cagccacgta
     4081 gcccgtcaac tcgtcgcccg gggagaagac gtccgcgtcc tcgtccgtgc ctccagctcc
     4141 aaccgcgcca tagccgactt gtcgctcgaa tacgtcactg gtgat
//
LOCUS       DATCBB010000054         1810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_326236, whole genome shotgun sequence.
ACCESSION   DATCBB010000054 DATCBB010000000
VERSION     DATCBB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326236"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VII37_00670"
     CDS             complement(<1..936)
                     /locus_tag="VII37_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5068257.1"
                     /translation="MPKNSSAQPENSEAKLRNETLGDFTTTRRVIPISALAMVIGLLC
                     AFVALALLRLIGLFTNLFYFGRWNTSLVSPAGNHLGIFSVLVPVAGALIIGVMARYGS
                     ERIRGHGIPEAIESILLNGSRIEPKIALLKPISSAISIGSGGPFGAEGPIIMTGGAVG
                     SMVAQLFHLTSAERKTLLVAGAAGGMSATFAAPVAAVLLAVELLLFEWKPRSLIPVAL
                     ASAVAAVARRYLLGFGPLFPVPTHPLFIGPMGLLGCALVGLLAGGLSALLTVSVYAAE
                     DAFQRLPIHWMWWPALGGLTIGLGGLIFPQALGVGY"
     gene            complement(929..1474)
                     /locus_tag="VII37_00675"
     CDS             complement(929..1474)
                     /locus_tag="VII37_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5068258.1"
                     /translation="MKNAISDTEYRALAELRYRIRHFIQEGDLAAQRSNLEPQQYLML
                     LAIRGLPLGAVATIRTLAERMALKHHSAVELIDRLESHGLVRRSRSERDKREVRVSLL
                     PQGSKLLDRVASERISELKASGVALADAITALVRRKHPSPIVAEINEMTEVLHRPAIS
                     RRGTPRKQNKKPMSHGRKRRA"
BASE COUNT          426 a          497 c          483 g          404 t
ORIGIN      
        1 gtagcctacg cccagcgcct gcggaaaaat taaaccaccc aggccaattg ttagtccgcc
       61 aagggcaggc caccacatcc agtgaatggg caagcgttgg aaggcgtctt ctgccgcgta
      121 gactgaaacg gtgagtaaag cggataatcc gcccgccaaa agtccgacta acgcacaacc
      181 caagagtccc atcgggccga taaaaagtgg atgggtgggg accggaaata acggcccaaa
      241 gcccagcagg taacgccgtg caacggcagc gacggcactc gccaaggcta ccgggatcaa
      301 gcttctgggc ttccattcaa aaagtaataa ctccacggcg agcagtactg ccgctactgg
      361 agcggcaaaa gttgcggaca tgcctccagc tgcgccggcg accaataatg ttttgcgttc
      421 cgcgctggtt aagtggaata actgggcgac catcgaacct accgcgccgc ccgtcatgat
      481 gattggtcct tccgctccga aaggtccgcc ggacccgatg gaaatggccg aggagattgg
      541 ttttagcaac gcgatcttcg gctcgatacg gctgccattt agcagaattg attcgatcgc
      601 ctccggtatt ccatgcccgc gaatgcgctc cgagccatac cgcgccatca caccgataat
      661 cagcgcgccg gcgacgggca ccaacacact aaaaattccc aggtgattgc cggcagggga
      721 aacgagtgat gtattccacc gtccaaagta aaataaattc gtgaagagac cgatcaggcg
      781 gagtaaggcc agcgctacga aggcgcagag taaaccgatc accatggcga gcgctgaaat
      841 ggggatcacg cggcgggtgg tggtgaagtc gcctagcgtc tcgttcctca acttagcttc
      901 ggagttctca ggttgcgcgg atgaattctt aggcacgcct ttttctccca tgtgacatcg
      961 gcttcttgtt ttgtttccgc ggtgtccctc tgcgcgagat cgctggtcga tgtagtacct
     1021 cggtcatctc gtttatttcc gcgactattg gtgaagggtg tttgcgtctc acaagtgcgg
     1081 tgatggcgtc ggcgagcgct acaccacttg ctttcaattc gctgatgcgc tcgcttgcga
     1141 cgcggtccaa caactttgag ccttgcggca gcagcgacac gcgtacttct cgcttgtcgc
     1201 gttcgctgcg gcttctgcgc accaatccat gcgattccaa gcggtcgatt aattcgacgg
     1261 cgctgtgatg cttgagcgcc atgcgctctg ccaatgtacg tatagtcgcc accgcgccca
     1321 gaggcaaacc gcgaatggcc aggagcatca ggtattgttg tggttccaga tttgaccgtt
     1381 gagccgccaa atccccttcc tgaatgaaat gccgtatgcg gtagcgcagc tcagctaggg
     1441 ctcgatattc ggtatcggaa atagcatttt tcatctgcct ctaaaagtat atcgtaatac
     1501 gatatttaaa ccaatgggat tggattgtac ggtgacgttg ccgctactaa ccgcgaatga
     1561 ccctcgctgc aaaaccaaaa aactcttcca agattccggc ttaatcttta cgacaattcc
     1621 ccaagaactg aggcggccga ctaagagaat gggacaagtg gcgcgtctcc actcgggaaa
     1681 tcaacatgtc gccgcacgac ctacatcact aggtgagtcg aaggttgaga ttccgttatc
     1741 ctttcccatt cattacggcg taaattatta tgcgattcag tattattccg tgtatgaatt
     1801 tgcgaatgtt
//
LOCUS       DATCBB010000055         1390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_99390, whole genome shotgun sequence.
ACCESSION   DATCBB010000055 DATCBB010000000
VERSION     DATCBB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99390"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VII37_00680"
     CDS             complement(<1..1268)
                     /locus_tag="VII37_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEY5068259.1"
                     /translation="MLPLSEVHPGMQGYAYTIFAGDQVEKFDLEVLGIMPNFLGPRQS
                     IILVQLKGPKVEHTGVVAGMSGSPVYLDGKLAGALSLKLGVFTKEPIAGVTPIADVLH
                     PPVQTSPSQPSQPAQSSQNSTQQFSPAYDASSNPSNNFAASQITLPTGSALEPIETPL
                     VFSGFQASALRQFSSQLQGYGFVAAQGGTAAPRPDDSQLKPGDMAGMVLVQGDASINS
                     ACTVTAVQADRVFLCGHPFLSLGDVQLPMARSRVVTTLSSDMASTKIVNMGGSIGTIT
                     GDHITAVTGKLGAPPAMIPLELTLSVAGAEKQLHFQLVNHPRLTPILVALTTFSGLTQ
                     NSLYGEGTTLHLSGEIQLKNHAPVQIENTFAPGDALSPDGLPIALTMQNIFARLFTNN
                     FESTSVERVSLRVESVPGRHSFTIESAWLE"
BASE COUNT          308 a          292 c          501 g          289 t
ORIGIN      
        1 ttttcgagcc aggcgctttc gatggtgaag gaatggcggc caggcacact ttcgacgcgc
       61 agagaaacgc gctcgacgga ggtggattca aaattattgg tgaacaaacg ggcaaaaatg
      121 ttttgcatgg tgagcgcgat aggcaggcca tcgggggaaa gagcgtcgcc aggagcgaag
      181 gtgttttcaa tttgaactgg ggcgtgattt ttcagttgga tttcgccgga gagatgtagg
      241 gtggtgcctt cgccgtagag cgaattctga gtgaggccgc tgaaggtggt gagcgcaacc
      301 aagatggggg ttaagcgcgg atgattgacc agctggaagt gcagttgttt ttccgcgcct
      361 gcgacggaaa gcgtgagctc cagcggaatc atggcgggcg gcgcgcccag ttttccagtg
      421 acagcggtga tgtgatcgcc agtaatggtg ccgatggaac cgcccatgtt tacgattttg
      481 gtggaggcca tgtcggagga gagagtggtg acgacgcggc tgcgtgccat gggcagttgt
      541 acgtcgccga ggcttaagaa gggatggccg cataagaaaa cgcggtcggc ttgcacggct
      601 gtgacggtgc aggcggaatt gatggacgcg tcgccttgca cgagaaccat tccggccatg
      661 tcgccaggtt tgagttgcga atcatcgggg cgcggcgcgg cggtgccacc ctgcgcagcg
      721 acgaagccgt agccctggag ttgcgaagaa aattggcgta atgcggatgc ttgaaaaccg
      781 gaaaagacca gtggggtttc gatgggttcc aaggcggagc ccgttggcaa agtgatttgt
      841 gaagccgcga aattatttga tgggttcgag gaggcgtcgt aggcgggcga gaattgctga
      901 gttgaatttt gtgaagactg ggcgggctgt gagggttgtg acgggcttgt ctgtactgga
      961 ggatgcaaaa catcggcgat gggcgttacg ccggcgatgg gttccttggt gaaaacgccg
     1021 agctttaggg aaagggcgcc tgcgagcttg ccgtcgagat aaacgggact gccactcatg
     1081 ccagccacga cgccggtgtg ctcgaccttg ggacctttta gttgaacgag gatgatggat
     1141 tgtctggggc ccaggaaatt gggcatgatg ccgaggactt cgaggtcaaa tttttcaacc
     1201 tggtcaccgg caaaaatggt gtacgcgtag ccttgcatgc cgggatggac ttccgagagg
     1261 ggcagcatct cggttgattc ttgagcccgt gaagcagggg ctacgagaaa gaaaaatgtg
     1321 agcgcgacga tggctagcaa gaggtgtagt tgaagcgagc ggggaatgga gtgatcaaaa
     1381 cgcgccggga
//
LOCUS       DATCBB010000056         1154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_294114, whole genome shotgun sequence.
ACCESSION   DATCBB010000056 DATCBB010000000
VERSION     DATCBB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294114"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VII37_00685"
     CDS             complement(<1..404)
                     /locus_tag="VII37_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5068260.1"
                     /translation="MLRWDQVEFATGALHVRRVKQGTPSTHPILGNELRALRRLQREQ
                     EPKSPFVFTSERGAPFSTAGFARMMERAGTAAKLNFKAHPHMLRHACGYALANKGHDT
                     RALQAYLGHRNIQHTVRYAELSPTRFRDFWRE"
     gene            complement(413..925)
                     /locus_tag="VII37_00690"
     CDS             complement(413..925)
                     /locus_tag="VII37_00690"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068261.1"
                     /translation="MMRKLPFLCTFGVLFSTIYWSNGAPVTDCDILAAAPIDSHRRIS
                     GISLDQIDVNRAVPACEAAVRRYPNDAHFRFQLSRAYYEAQNFQAAVAQLRKAAEQGY
                     ALAQYNLGVMYEYGKGVSEDYGEALVWYRKAADQGDEMAKNKLDRLTSIDPDTTTKSF
                     GREPKQYFQS"
     gene            complement(961..>1154)
                     /locus_tag="VII37_00695"
     CDS             complement(961..>1154)
                     /locus_tag="VII37_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068262.1"
                     /translation="PVCRNCLFVPLCRGGCRCDWSLVETPFGSMDYLADPTNIDGAES
                     GAASARASSPPALSSVAAA"
BASE COUNT          254 a          317 c          325 g          258 t
ORIGIN      
        1 tattcgcgcc agaaatccct aaatcgcgtt ggcgagagtt cagcgtagcg cactgtgtgc
       61 tgaatgttgc ggtggccgag gtatgcttgg agggcgcgcg tgtcgtgccc cttattggca
      121 agggcataac cgcaagcgtg ccgcaacatg tgcgggtgcg ccttgaagtt tagcttggcc
      181 gcggtgcccg cgcgctccat catgcgggcg aaccctgccg tgctgaacgg cgccccacgc
      241 tccgaggtga acacgaatgg cgattttggt tcctgttcgc gctggagccg ccgcagcgcc
      301 cgcaattcgt tcccgaggat tggatgggta ctcggggtgc cttgtttcac tcggcgcacg
      361 tggagagccc ctgtggcgaa ctcgacctga tcccagcgta gcactaccgc ttttatgatt
      421 gaaaatactg tttcggttcg cgcccaaaag atttagtcgt tgtgtctgga tcaattgaag
      481 taaggcggtc aagtttgttc ttcgccattt catctccttg gtccgcagcc ttgcgatacc
      541 aaacaagagc ttcaccgtag tcttcgctga cacccttacc gtattcatac ataacgccaa
      601 gattatactg cgccaacgca tatccctgtt cggcagcctt tctgagttga gcaactgcag
      661 cttggaaatt ttgagcttca taataagctc gactaagttg aaagcgaaag tgagcgtcat
      721 ttgggtatcg ccgcacggcg gcttcacacg cggggacagc cctattcaca tcaatttgat
      781 ccagcgaaat accagagatt cggcgatgtg aatcgatggg agcagcagcc aaaatatcac
      841 agtcggtgac gggcgcacca tttgaccaat agatggtgct aaaaagaacc ccaaaagtgc
      901 aaagaaaggg tagctttcgc atcattgtcg cctttgttgg gtaactatcc tcggtcctcg
      961 tcatgctgcc gcaacggagg atagcgcagg cggagacgac gcgcgggcgc tggcggcccc
     1021 tgattcggca ccgtcaatat tcgtgggatc ggcaagataa tccatgcttc cgaagggcgt
     1081 ctccaccaac gaccaatcgc accgacaccc tccgcggcac agtggaacga ataggcagtt
     1141 gcgacagacg ggcg
//
LOCUS       DATCBB010000057         3257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_228921, whole genome shotgun sequence.
ACCESSION   DATCBB010000057 DATCBB010000000
VERSION     DATCBB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228921"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /locus_tag="VII37_00700"
     CDS             <1..2177
                     /locus_tag="VII37_00700"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5068263.1"
                     /translation="FVCASTAGSAMSQDTRALEETPVVSGYEQQLAGEIRSSIKGFTP
                     QTDSLGNVYVTIGSGAPRRMIVAPIDQPGYVVSEIALDGYLRVQRLPQRAPNAVFDLL
                     HAAQPVWVMTREGKKVAGVFSGLSVHLQPGRQNAPTMAHPDEMYVDIGAASAEEARAS
                     GVDVLDPISLSQYPEVIGTGEMAGPAVGDGFGRSVLLELLGLLANHKVNLPGTLTIAF
                     ATQQWTGGRGLDRLLNELHPDELIYVGRIMPPHIDESKPAPATTREAMALGSGVLIGV
                     SDASAPPAGLAAELKKIGDSHNVRIQPVTAALPALTSYAKSTPWPARFAHLSVASLYP
                     VTPAETISTADISALESILYQYLGLQGGIAGGQVGGAFGYRPSALRVLTETYGASGHE
                     EAVREQVKKSLPSWAKPETDAAGNLVLKMGSAKADSKARKLVFIAHMDEIGYQVRSIE
                     PDGRLLMDVLGGGYTEYFLGHVMLLHKRDGSTVGGVLELLAKWDQPGFEWPHGPKAMD
                     EPAHMYVGTHSAEETQKLGIKVGDWATVPKKYRQLIGTRANARSFDDRVGCAALIEAV
                     NTLGPNLGDRQVTFIWSTEEEVGLKGAATAAERMAKEGDSPDFVFAIDTFVSSDSPIE
                     SKRFADAEIGKGFVIRAVDNSNITPREYVDRVVKLANDNKIPVQYGVTGGGNDGAVLV
                     RYGAVDVPLSWPLRYSHSPAEVIDTRDYDALAKIVAVLAKQW"
     gene            complement(2365..>3257)
                     /locus_tag="VII37_00705"
     CDS             complement(2365..>3257)
                     /locus_tag="VII37_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068264.1"
                     /translation="IQRNALKSWKLLHPDVEVILFGDDAGSAEIARELGLRHEPFVER
                     NSFGTKRLDYIFGRAQELARHDFLCYCNCDIILLPEFCQALKRVADTHPRFLMVGRRW
                     DTDITEAIDSDVPDWAQRAKTLALDHGVQQPGYSVDYFAFRRGLYASMPPPLVIGRIW
                     WNHWLVWKARQLGAAVVDASDQVTAIHQNHNYGYHPAGARGVWTDEQAAENYRLAGGR
                     WNLFTIDDATHILRDRGECTSLARTLWCPRIAAWDTASKMTRKDWLPARFEWKPFQWM
                     NMCVNPARRISSNVMWRAQK"
BASE COUNT          761 a          875 c          915 g          706 t
ORIGIN      
        1 gatttgtatg tgccagtacc gccggctcgg ccatgtccca agacacccgc gccctggaag
       61 aaacgcccgt ggtaagcggg tatgaacagc agttggccgg cgaaatacga agctcgatca
      121 aaggctttac accacagacc gacagtcttg gcaatgttta cgtcacaatc ggcagcggcg
      181 ctccgcgccg catgattgtc gcgcctatcg atcaaccggg atatgtagtt agtgaaatcg
      241 ctctggacgg ctatcttcgc gtgcagcgtc ttcctcagcg tgcgccgaat gccgtgtttg
      301 atttattgca tgccgcccaa ccggtttggg tgatgacacg cgaggggaaa aaagttgctg
      361 gagtattctc tggtctctcg gtgcacttgc aacctggccg ccagaacgcg ccgacgatgg
      421 cgcaccctga cgagatgtat gtcgacatcg gtgccgcgag cgctgaggaa gctcgcgcct
      481 caggtgtgga cgtactggat ccgatttcac tttcgcaata tccggaggtt attggcacag
      541 gcgaaatggc cgggccggca gtcggcgatg gctttggacg atcggtgctc ctcgaattgt
      601 tgggcctgct tgcaaaccac aaagttaatc ttcctggaac gctcacaatc gcgtttgcaa
      661 cccagcagtg gaccggaggt cgcggactcg accgcttgct gaatgaattg catcccgatg
      721 agctgatcta cgtgggccgc atcatgccgc cacacattga tgaatcaaaa ccggctcccg
      781 ctaccacgcg cgaagcaatg gcactcggta gcggtgtttt gatcggtgtt tccgatgcct
      841 ctgccccgcc agcgggcctc gcagcagaac tgaaaaaaat cggagatagc cataatgtga
      901 gaatccagcc ggtgactgcg gccctgcccg cgctgacaag ttatgcgaag tccacgccct
      961 ggcctgcgcg ctttgcgcac ttgagtgttg ccagtctcta tcctgttacg ccggccgaaa
     1021 ccattagcac ggctgacatc tcagcgctcg aatcgattct ttatcaatac ctcggattac
     1081 agggtggcat agccggcggt caagtcggcg gagcatttgg atatcgtccc agcgcccttc
     1141 gagtactgac agaaacatat ggtgctagtg gacatgaaga ggccgttcgc gagcaggtca
     1201 agaaatcatt gccaagttgg gccaagccgg aaacagatgc ggctggcaat ttagttttga
     1261 aaatgggaag cgcgaaggcc gattcgaaag cgcggaaact cgttttcatc gcgcatatgg
     1321 atgaaatcgg ctaccaggtg cgttccatcg aacctgatgg gcgtttgctc atggacgtgc
     1381 tcggaggcgg ttacacggaa tattttctcg gccacgtgat gttgctgcac aaaagggatg
     1441 gcagcaccgt tggcggagta ttggagctgc tcgcgaaatg ggaccagccc ggattcgaat
     1501 ggccgcacgg accaaaagcg atggacgaac cggcgcatat gtacgttggc acgcattcgg
     1561 ctgaagaaac gcagaagctc ggaattaaag taggcgattg ggccacggtc cccaaaaaat
     1621 accgccaact cattggaacg cgagcgaacg ctagaagttt tgatgatcgt gttggctgcg
     1681 ccgcattgat tgaagcagta aatacgctgg gcccgaattt gggcgaccgg caagtcacat
     1741 tcatctggtc gacggaagaa gaagtgggat tgaagggcgc tgcaactgcc gcggaaagaa
     1801 tggccaagga aggcgatagc ccagattttg ttttcgccat cgatactttc gtcagttccg
     1861 attcgccaat cgagtcaaaa cgttttgcgg atgccgaaat cggcaaagga ttcgtgattc
     1921 gcgctgtgga caactccaac attactccgc gcgagtacgt ggaccgcgtc gtgaaactgg
     1981 ccaacgacaa taaaatcccg gtgcaatatg gcgtaaccgg tggcgggaat gatggcgcag
     2041 ttttagtgcg ttatggtgcg gtggatgtgc cgttaagctg gccgctgcgc tattcgcatt
     2101 cgcctgcgga agtcatcgac actcgcgatt atgacgcgct ggctaaaatt gtggccgtcc
     2161 tggcgaaaca gtggtgagtt aaagcggcgc taaagaatta tttcgagcgc tgagcgagaa
     2221 tatgattatc gccgcatggt tcacagcggt aaccaagatc tgcaaatgac ttcagcagcg
     2281 ttcgccggtt ttcgtcgcca tgcatctcgc atagaataag cggcggtctt tcgtgtaaga
     2341 gtctttctgc gccgcgaaat acttctactt ctgcgccctc cacatcacat ttgatgaaat
     2401 ccggcgcgcc ggatttacac acatattcat ccactgaaat ggcttccact cgaatcgtgc
     2461 cgggagccaa tctttcctcg tcatttttga tgccgtatcc caagccgcga tccggggaca
     2521 ccacagcgtc cgcgcgagcg aagtacattc tccgcgatca cgcaagatgt gagtggcatc
     2581 atcgatggta aacaagttcc accgtccgcc cgccagtcga taattttcgg ccgcttgctc
     2641 gtcagtccaa acaccacggg ctccagcggg atggtaaccg tagttatgat tttgatgaat
     2701 ggcggtcact tggtcggagg catcgactac tgccgctccc agttgacgag ccttccaaac
     2761 cagccagtga ttccaccaga tgcggcctat caccagtggt ggcggcatgg acgcataaag
     2821 cccgcggcga aaagcgaaat aatccacaga ataaccaggt tgctgaacac cgtgatcgag
     2881 cgcgagagtc ttagcacgtt gcgcccagtc cggcacatca gaatcaatcg cttcggtgat
     2941 gtccgtgtcc caacggcgcc ccaccatcaa gaatcgcggg tgcgtatccg ccacgcgctt
     3001 cagagcctgg caaaattctg gcaacagaat aatgtcgcaa ttgcagtaac aaagaaaatc
     3061 gtgccgggcc aattcttgcg cgcgaccgaa aatgtaatca agtcgcttgg tgccaaaact
     3121 gttgcgttct acaaaaggtt cgtgacgaag gccgagttcc cttgcaatct ccgcgctgcc
     3181 cgcgtcgtcg ccaaagagaa taacttcgac atctggatgc agcagcttcc agcttttgag
     3241 cgcgttgcgt tgaataa
//
LOCUS       DATCBB010000058         1244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_196565, whole genome shotgun sequence.
ACCESSION   DATCBB010000058 DATCBB010000000
VERSION     DATCBB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196565"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1244
                     /locus_tag="VII37_00710"
     CDS             <1..>1244
                     /locus_tag="VII37_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5068265.1"
                     /translation="AVNRSFADLVATPYENIVGKPLSDFVQEAAGAGHDLVKRAMPRF
                     LERRSWSGVVNVHLKNQQGIFYFDCVANAMMRDDKVHGVTVLARDVSALRRNEARFTE
                     LFETLQEGIYIINLNGDILDANPALVRMLGYDSKEELLARRVPDVFHDRNERKSLKNE
                     VERQPILQGREITLTRKDGTSLICLNTAAAVRDSAGSVIRYQGALMDVTASRVMERRL
                     HQQQEFARRLIDSFPDLIFVLDNAASYTFVSPRVLEVLGFEPEEIRETELGSRTHTED
                     RGALRAMYDEILAGRETFASVEARVRNKKGDWRRLRFHFSPLFDEMDKIDGVIISGRD
                     VTDLKRLEEQLIQAEKLAAMGQMLAGVAHELNNPLTAILGVTELLRERSGADETMQRQ
                     LELTHRQARRAARIVQNLLEFS"
BASE COUNT          272 a          353 c          345 g          274 t
ORIGIN      
        1 gcgcggtcaa tcgcagcttt gccgatcttg tcgctactcc ctacgaaaac attgtaggca
       61 aacctctgtc cgatttcgtt caggaagcag ctggcgcagg ccatgatctc gtgaagcgcg
      121 ccatgcccag atttctggag cgccgcagct ggtctggcgt ggtgaacgtg cacctcaaaa
      181 atcagcaggg cattttttat ttcgattgcg tcgcgaacgc catgatgcgc gacgataagg
      241 tccatggtgt caccgtgctc gcgcgcgacg tcagtgcttt acgcagaaac gaagcgcgtt
      301 tcacggagct ctttgaaact ttgcaagagg gcatctatat catcaatctg aacggcgaca
      361 tccttgacgc aaatcctgcc ttggtgcgca tgttgggtta tgactccaag gaagaactgc
      421 tcgcccgccg ggtcccggac gtctttcatg atcgcaatga acgcaagagc ctgaaaaacg
      481 aagtcgagcg ccagcctatc ttgcaaggcc gggaaattac tctgacccgc aaagacggca
      541 cttccctcat ctgcctgaac accgctgctg cagtgcgcga cagtgctggc agcgtgatcc
      601 gttatcaagg cgcgctcatg gatgttaccg ccagccgtgt gatggagcgc cggctccatc
      661 agcagcagga atttgcgcgc cgcctgatcg atagttttcc agatttgatc ttcgtgctgg
      721 ataacgccgc tagctacacc ttcgtcagtc ctcgcgtcct ggaagtattg ggttttgagc
      781 cggaggaaat acgcgagacc gaactaggtt cgcgcaccca tactgaagat cgaggcgcgc
      841 tgcgcgccat gtatgacgaa attcttgctg gccgtgaaac atttgcctcc gtcgaagcgc
      901 gcgtgcgcaa caaaaaaggc gactggcgcc gtctgcgctt tcatttcagc ccactcttcg
      961 atgaaatgga caagatcgat ggcgtgatta tttccgggcg cgatgtaact gacctcaagc
     1021 gacttgagga acaattgatt caggcggaaa agcttgcggc catgggccag atgcttgccg
     1081 gcgtggccca cgaactaaac aatccgctca ccgctatcct cggtgttacc gagctactcc
     1141 gcgagcgctc tggcgccgat gagactatgc agcgtcagtt ggaattgaca caccggcaag
     1201 cccggcgcgc cgcgcgcatc gtgcagaatt tgttggaatt ttcc
//
LOCUS       DATCBB010000059         1166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_131865, whole genome shotgun sequence.
ACCESSION   DATCBB010000059 DATCBB010000000
VERSION     DATCBB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131865"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VII37_00715"
     CDS             <1..971
                     /locus_tag="VII37_00715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5068266.1"
                     /translation="IVAEGFNSTVSINRDGKFMAFERTSLTMPAEVFVATGDGTNAQQ
                     VTHHNDALLAKLEMNAPETFWFEGAGGSRVQAMLIRPPNFKATKKYPLLVLLHGGPQT
                     MWSNAWGYRWNAQVFSAAGYVTLMINRHGSTGYGQKFTDEITNDWGGQAYTDVMKGVD
                     YALANNKFIDGSRMAAAGGSYGGFMADWIATHSGRFKAIISHAGVYDQTSMYATEELW
                     FAEHDMQGTPWSNPESYKKWSASTFAGELGKFKTPTLVIAGENDFRVPYTQSLEFFNT
                     LQREGVPSKLVIFPDEGHWVLKPQNSQFWYKTFLEWLAMYLKKEAQ"
     gene            complement(983..>1166)
                     /locus_tag="VII37_00720"
     CDS             complement(983..>1166)
                     /locus_tag="VII37_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEY5068267.1"
                     /translation="FSNWILSFGEDLLAVWLTWMARAHPLTTAIVVGALLILSAFLIF
                     HLFRFARRAFRRSSTA"
BASE COUNT          304 a          280 c          353 g          229 t
ORIGIN      
        1 aaatcgtggc cgaaggattc aacagcacgg tgtcgattaa tcgggatgga aagttcatgg
       61 cgttcgagcg caccagcctg acgatgccgg ccgaggtatt cgtggctacc ggagatggca
      121 cgaacgccca gcaagtgacg catcacaatg atgccttgct ggccaagctg gaaatgaatg
      181 cgccggaaac attctggttt gagggagcgg gtggctcgcg cgtgcaagcg atgctcattc
      241 gtccgccgaa ttttaaggcc acgaaaaagt atccgctgct ggtgctgctg catggcgggc
      301 cacaaacgat gtggtcgaac gcctggggtt acagatggaa cgcgcaggta ttttcagccg
      361 ccggttacgt gacgctgatg atcaaccgcc acgggtccac cggctatggc cagaaattca
      421 ccgatgaaat caccaatgat tggggcggcc aagcgtatac cgacgtgatg aagggcgtgg
      481 attacgcgct ggccaataat aaatttatcg atggttcgcg aatggccgcg gcgggtggtt
      541 cgtacggggg atttatggct gactggatcg cgacgcactc cgggcgattc aaagccatca
      601 tcagccacgc gggcgtttac gaccagacct cgatgtacgc tactgaagag ttgtggttcg
      661 cggagcacga catgcaagga actccgtgga gcaatccaga aagctacaag aaatggtcgg
      721 catccacgtt cgcgggagag ttagggaaat ttaaaacgcc aacgctggtg atcgccggag
      781 aaaatgactt tagggtgccg tacacgcaat cactggagtt ttttaacacg ctgcagcgcg
      841 aaggggtgcc gtccaagttg gtaattttcc cggacgaagg ccactgggtg ttgaagccgc
      901 agaattcgca attctggtac aaaacttttc tggagtggct ggcaatgtat ttaaagaaag
      961 aagcgcaata agctgcggcg agtcaggctg tcgacgagcg acgaaatgcg cgacgcgcga
     1021 acctgaataa atggaagatc agaaaagcgg agagaatcag tagtgcgccg accacgattg
     1081 cggtggtcag cggatgagcg cgagccatcc aggtaagcca caccgcgagt aaatcttctc
     1141 caaaacttaa aatccaatta ctaaac
//
LOCUS       DATCBB010000060         1449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_164335, whole genome shotgun sequence.
ACCESSION   DATCBB010000060 DATCBB010000000
VERSION     DATCBB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164335"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..>1449
                     /locus_tag="VII37_00725"
     CDS             111..>1449
                     /locus_tag="VII37_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068268.1"
                     /translation="MMEALLQKSAVVESKTAQRKVARPAATPNLAGIQAESVRLPLCF
                     VLAGVLSLLVSAGFVLGQPDLLAMYHYTPHLIAITHLFVLGFICSIVMGAMYQLVPVT
                     LETQLFSERLMKWQFAAHVIGFTGMVWAFWNFNMALVGAFGSLLTLDVGLFVFNLACT
                     LAKVKKWNTIKFGIASALFWLSSTVAAGLFLTASKCWNFSSFQPLAAMHAHAHLGVVG
                     VFVMMIVTVSYKLVPMFTLSELQNERRAWWSVALLNVGLLGTFVAVLLQSPLKPVFAL
                     MIIAGFALYGLEIKAILRARNRRTLDWGMRYFLTAIALLVPLSILALVLSRSGLPLTA
                     FTMQLENVYGLLALGGLVGLAILGMLYKIVPFLVWFHSYSRHIGRSKVPALAEMYSVP
                     LQIAGYWTFLVGLAVLCVATALSREAGIRWGGCLLALSLAIFAVNMGKILSHFV"
BASE COUNT          270 a          386 c          408 g          385 t
ORIGIN      
        1 tgcggtcttg cctgctggtg cggaactgcg cgcccacacg gaacgccgcc cgatgcacct
       61 ttacgcccaa ctcgaagaac gcggatttgt cggtgaaacc gaggaacaaa atgatggaag
      121 ctttattaca aaaatccgcc gtcgttgaat caaaaactgc gcagcgaaaa gtagcccgcc
      181 cggcagcaac gccgaatctc gccggcattc aagcagaatc ggttcgcttg ccgctgtgtt
      241 ttgttctggc cggtgtgttg tcattgctgg tcagcgcagg atttgtattg ggacaaccgg
      301 atttgctggc gatgtatcat tacacgccgc acctcatcgc aatcacgcat ttgttcgtgc
      361 tgggtttcat ctgttccatt gtcatgggcg cgatgtatca actggttccg gtgactttgg
      421 aaacgcagct attcagcgaa cggctgatga aatggcagtt tgccgcgcac gtcatcggtt
      481 ttaccggcat ggtctgggcg ttctggaatt tcaacatggc tctggttggc gcgtttggct
      541 cgttgctgac attggacgtt ggactattcg tcttcaactt ggcttgcact ctggcgaaag
      601 ttaaaaaatg gaacacaatc aagttcggta tcgcctcggc gctgttctgg ctgtcgtcca
      661 ctgtcgccgc cggacttttt ttaacagcat caaaatgctg gaacttcagt tcgtttcagc
      721 cgctggccgc catgcacgcg cacgcacacc ttggcgtcgt tggcgtgttc gtgatgatga
      781 ttgtcacggt gagctacaag ctcgtgccga tgttcacctt gagcgaactg caaaacgaac
      841 gccgcgcgtg gtggtcggtc gcattactta atgtcggctt gctcggcaca tttgtcgccg
      901 tgcttttaca aagtccatta aagcccgtgt ttgccttgat gataattgct gggttcgcgc
      961 tttatggctt ggaaattaaa gcgattctgc gcgcgcgcaa ccgccggacg ctggattggg
     1021 gaatgagata ttttctgacg gccatcgcgc tgctcgtccc attgtccatt ttggcgctcg
     1081 tgctgtcgcg gtcggggctg ccgttgaccg cgttcacgat gcaattggaa aatgtctatg
     1141 gcctgctcgc gctgggcgga ctcgtgggac tggcaattct aggaatgctt tacaaaatcg
     1201 tgccgttcct cgtttggttt cacagttaca gccgtcacat cggccgctcc aaagtgccgg
     1261 cactggctga gatgtattcc gtgccgctcc aaatcgcggg ctactggacc tttctggtcg
     1321 gactcgccgt tttatgcgtg gcgaccgcgc tgtcgcgcga agcgggaatt cgctggggcg
     1381 gctgcctact tgcgttgagc ctcgcgattt tcgccgtcaa catgggcaaa atcctttcgc
     1441 attttgtcc
//
LOCUS       DATCBB010000061         1216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_326390, whole genome shotgun sequence.
ACCESSION   DATCBB010000061 DATCBB010000000
VERSION     DATCBB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326390"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..643
                     /locus_tag="VII37_00730"
     CDS             398..643
                     /locus_tag="VII37_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068269.1"
                     /translation="MTGLAMTLIKKRDIEKHFAARRRARTHPFTPTSQPDATGFSGPA
                     PDSIPVKQKGFAKDFLGEHSVPVKPIVPGAIEGPGAL"
     gene            783..>1216
                     /locus_tag="VII37_00735"
     CDS             783..>1216
                     /locus_tag="VII37_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068270.1"
                     /translation="MAIPETMYPEYPASGPASAACHSAALKEFTLPLPTRIAFVGNYL
                     PRECGIATFTTDLCSALGTEYGEGRLFAIPVNDPDSSYEYPKQVRLELEQEDIASYER
                     AADFLNFNGNDLVCLQHEYGIYGGTAGRHILALLRRLKMPLVT"
BASE COUNT          264 a          345 c          357 g          250 t
ORIGIN      
        1 gagttcttca acattctcaa ccacaccaac ttctccacgc caaacgaggt ggtgtacacc
       61 tcggcgacgt cggggatttc acccacggca ggcgtagtta ccgcgacttc cacgacgtcg
      121 cggcagattc aattcggagc caagatccag ttttagagca atattgaaca gtaggaaggg
      181 ccgctcgcgg ggtaggttcg aggagcggcc ttttcttttg cggctgctcg ttattggcgc
      241 gccagacacg atttagggtg cgatagggga acttgtggat gcgcaggcac gcaatctgca
      301 aagcgcgtaa gctcaaggta gtagttaaag tctcccccgc tcgcctccgg cattccaggc
      361 ttcgcttcag acgagcaccg ctcaagtact caaccgaatg actggacttg ccatgaccct
      421 catcaagaag cgcgatatag agaagcattt tgctgcgcgg cggcgcgcac gcacacatcc
      481 ttttaccccc acaagtcagc ccgatgcgac cggcttttca gggccggcgc cggactctat
      541 tcccgtgaag caaaagggct ttgccaaaga cttccttggt gagcattcag ttccggtaaa
      601 gcccatcgtg ccgggcgcaa tcgaaggtcc tggggctctg tagagcctgg gggctcgaat
      661 cgcgcgccag ccggattcgg cagcggactt cgagggagaa gtctggaaag cgataacggc
      721 gtggacgtct cgcgcaactg gccgattgct cgcgacacag aaatagttga aagcaggaca
      781 caatggcgat ccccgagacg atgtatcccg agtatccggc aagcgggccc gcttccgcgg
      841 cttgccattc cgcggcattg aaggagttca ctttgccttt gcccacgcgc attgcgtttg
      901 ttggaaatta cctgccgcgc gagtgcggta ttgctacgtt taccaccgac ctgtgcagcg
      961 cgctggggac agaatacggg gagggcaggc tgtttgcgat tcccgtcaac gaccccgatt
     1021 cgagctacga gtatccgaaa caagtgcgat tggaattgga gcaggaagac attgcatcgt
     1081 acgagcgggc cgcggatttt ctcaacttca atggtaacga cctggtgtgc ctgcaacatg
     1141 aatacggcat ttacggcgga accgcaggcc ggcacattct cgccttgctg cgccggctga
     1201 agatgccgct ggtgac
//
LOCUS       DATCBB010000062         1059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_196721, whole genome shotgun sequence.
ACCESSION   DATCBB010000062 DATCBB010000000
VERSION     DATCBB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196721"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1059
                     /locus_tag="VII37_00740"
     CDS             <1..>1059
                     /locus_tag="VII37_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEY5068271.1"
                     /translation="QIRFGTDGWRGIIADDFTFENVRRVAAAISSYVLKHEDPRRGLI
                     VGYDTRFASKPAAYIAAEVIASAGIPVILASDYMPTPAISLAVKQRGAAGGVIITSSH
                     NPWNWNGVKFKGNFGGSATPAIMKIIEDELHTGVMPNSQKAKIDEVDLKESYIEAISG
                     FVDLKLVAKANFKFAVDSMYGSGRGVLAKIFEERGISHVAIHQELNPLFPGINPEPIE
                     PHVALLQQTVVKQGCHAGFATDGDADRIGAVAEDGSFVDSHKIFSVILRWLLERKKWP
                     GEVVRAFNTTRMLDRIAARYGRKLNECPIGFKYIVDLMMEREILIGGEESGGIGYSRF
                     LPERDGILNSLVLANVMA"
BASE COUNT          250 a          272 c          297 g          240 t
ORIGIN      
        1 acaaatcagg ttcggtacgg acggctggcg cggcattatt gccgatgatt ttacgtttga
       61 gaacgtgcgg cgcgtcgctg ctgcgatttc atcctacgtg ttaaagcacg aagacccgcg
      121 ccgaggattg atcgtcggtt acgacacgcg ctttgcgtcc aagcccgcag cgtatattgc
      181 tgccgaggtg atcgccagcg ccggaatccc agtgatcctt gccagtgact acatgccaac
      241 ccccgccatt tcgctggcag tgaagcaacg cggagcagcc ggcggcgtga ttatcacgtc
      301 cagccacaat ccctggaatt ggaatggagt caagttcaaa ggcaattttg gcggctccgc
      361 cactccagca atcatgaaga tcatcgagga tgaacttcat accggcgtga tgccgaactc
      421 tcaaaaagcc aaaatcgacg aagtggattt gaaagagtcg tacatcgaag ccatttcagg
      481 gtttgtggac ctcaaacttg tagccaaagc gaattttaag tttgccgtgg attctatgta
      541 tggctcggga cgcggcgtgc tggccaagat atttgaggag cgcggcattt cccatgtggc
      601 gattcaccag gaactgaatc cgttgttccc cggcattaat ccagagccaa tcgaaccgca
      661 cgttgctttg cttcagcaaa cagtcgtcaa acagggctgt catgctggtt tcgcaaccga
      721 tggagacgcg gatcgcatcg gcgctgtcgc cgaagatggc agctttgttg actcgcacaa
      781 aatattttcc gttatcttgc gttggttgct ggagcgcaag aaatggcccg gcgaggtagt
      841 ccgcgcattc aacacgacgc gaatgttgga tcgcattgcc gcccgctatg gccgcaaatt
      901 gaatgagtgc cccataggct ttaaatatat tgtcgacctg atgatggaac gagaaatact
      961 gataggcggc gaggagtcgg gaggaatagg gtactcacgc tttctaccgg aacgcgatgg
     1021 catcctaaat tcgctggtgc tggccaacgt catggccga
//
LOCUS       DATCBB010000063         6327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_261829, whole genome shotgun sequence.
ACCESSION   DATCBB010000063 DATCBB010000000
VERSION     DATCBB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261829"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VII37_00745"
     CDS             <1..1100
                     /locus_tag="VII37_00745"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY5068272.1"
                     /translation="APRYHGAWSHFMDGHTGQTLPVFDMVDDGGDLVETAFLMEGLLT
                     ARQYFATHHGHETNGHAGENDSKGPELFSRITKLWEEVEWDWYKKSPQGEALFWHWSP
                     DFAWHISNRLTGFNETMIVYLLAMASPTHAISPELYYTGWANQKHAAVGSSIDDPRPG
                     PRYTNGRKYFGIKLDVGWGTGGPLFFTHYSYMGFDPHAATDRFTNYFENNRNTALINR
                     AYCIANPKHFEGYSAESWGLTASDGPEGYNPSAPDEDDDHGLMAPTGALSSFPYTPEA
                     SMAALKHFYRDEGSWLWGVYGPRDAFDAHGHWVSPIYMGLNQAPITVMIENYRTGLIW
                     KWFMSNPELEPMLAKAGLQMHTHGTKRDSTQ"
     gene            1207..2532
                     /locus_tag="VII37_00750"
     CDS             1207..2532
                     /locus_tag="VII37_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5068273.1"
                     /translation="MSEIPSVTVAGSALQELLPPLSEQEAVTESARCLMCFDAPCTHA
                     CPTHIDIPKFIKKISTGNLLGSARTILESNLLGATCARVCPVQELCEGACVLGSEHKP
                     IAIGRLQRHAMDYVYERKIDVFHAGKSIGKKVAVVGAGPAGLSCAGELSKLGVSVTLY
                     EKRELPGGLSTYGIIVLREPVEVALAEVAMIQRLGSRIEPGKEVGVDPGVLDLQKNFN
                     AVFIGVGLGNSPELGIPGEEYILDGLRYIEDSKLQPAQLQVGRNVIVIGAGNTAIDCA
                     TVARRLGAERVTMLYRRTEKEMTAYPHEYAFVKLEGVSVSFLFKSVKVHHENGVVKAL
                     ECVRVALGEVDASGRAAPKEIPGSNVLLPADQIVKAIGQNKLSLASQLDLKTERGFIR
                     VDSNFETSSPRIFAGGDCIRARGSASTVMAVQDGKLAARAIYQRLKNHG"
     gene            2525..3910
                     /gene="preA"
                     /locus_tag="VII37_00755"
     CDS             2525..3910
                     /gene="preA"
                     /locus_tag="VII37_00755"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEY5068274.1"
                     /translation="MADLRINFAGIRSPNPFWLASAPPTNSGAQIHRAFEAGWGGAVW
                     KTIGAPVLNVSNRYGSWRYGNEKMLAINNVELISDRPMEVNLREIAEVKRAWPDRAVI
                     VSAMVESKPESWHDVIRRIQDTGADGIELNYGCPHGMSERGMGSAVGQVPEYCEEITG
                     WVMEVAKIPVIVKLTPNISDIVMPARAAVAAGANAISLINTINSIMGVDLDTLDITPS
                     IGGKGGHGGYAGPAVKPVALNMLAALGRDSVVHGSGLPISGMGGISTWRDAAEFLLLG
                     ASSLQVCTAVMHYGFRIIEDLCDGLSNWMDAKQLATIPDVVGHSLAQVSDFKDFDLSF
                     KVVARIDQQKCIRCDLCYVACNDAAHQCIDLIDKQGHVTGPTAYEPSSNGRVEAVSTR
                     PNVVVREPDCVGCRLCFNICPVENCIHMVEVRPEQPSITWDELTAKQPEVAEDWDQME
                     RYRQKMGIRIH"
     gene            3931..5310
                     /gene="hydA"
                     /locus_tag="VII37_00760"
     CDS             3931..5310
                     /gene="hydA"
                     /locus_tag="VII37_00760"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY5068275.1"
                     /translation="MGLLIRNGEIVTVGSRTRADIYAENETVTRIGKNLDVPHGTEII
                     DAAGKFVFPGFIDPHVHIYLPFMATFAKDTHETASIAALIGGTTTYIEMCCPNRTDDA
                     LAGYELWKSKARGKSACDYAFHMSVTKFGEETKSQLREIVADGTSSFKIFLAYKNFFG
                     VDDGEMYQTLTLAKKLGVIVTAHCENAELVARLQAQLIAEGKTGPEWHEPSRPESVEA
                     EGTGRFATFLENTGATGYVVHLSCERALNAALAAKERGVHLSVEAVLPHLLLDKSFAE
                     RPGVDGMKHVMSPPLRAKQNQDVLWDALARGFVDTVGTDHCPFDVEQKLLGKDAFTNI
                     PNGIPGIEDRVNLLYSYGVQKGRIDLDRFVNIASTRAAKLFGLFPRKGTIAVGSDADL
                     VVFDTNYRGIVSAKTQHVNNDYNGFEGMAIEGRPAVVTVRGKVQVREGEFVGERGRGR
                     LLKREPANF"
     gene            5322..>6327
                     /locus_tag="VII37_00765"
     CDS             5322..>6327
                     /locus_tag="VII37_00765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEY5068276.1"
                     /translation="MSLDPKRTVAELKELRSLTGDENGAQRVAWTNTWLRAREWFAKK
                     LAELPVEHHYDAAGNNWATLAGESKKSLLLGGHLDSVPNGGWLDGALNVLAALAVLRR
                     FASEFNGRPPVTIRLVDWADEEGARFGRSLLGSSAFAGTHSISADRVRTDRDGIKMEE
                     ALRRCGVEMDRFAEAGKERQDAAAYLELHIEQGPVLEQLGLPLAVVLGTKGVERHSII
                     FHGQEAHSGSTPMNARHDALAAAAKLALQIRNIASKHPDAVCTMGSLKTFPGIVTAVV
                     GRCEATLDQRDLDARILAQMFEEAQRASRRFAEEEKCTVEWSRIWNIEPVPFHPELLD
                     L"
BASE COUNT         1396 a         1604 c         1902 g         1425 t
ORIGIN      
        1 aggcgccacg ctatcacggg gcttggtctc attttatgga cggccacacc gggcagacgt
       61 tgccggtttt cgacatggtc gacgacggtg gcgacttggt ggaaaccgcc tttctgatgg
      121 aaggattact aaccgcgcgg cagtattttg cgactcatca cgggcatgaa acgaacggcc
      181 atgcgggcga gaacgattcc aaaggaccgg agcttttttc gcgcataacc aaactgtggg
      241 aggaagttga gtgggactgg tacaagaaaa gccctcaggg ggaagcactg ttctggcatt
      301 ggtcgccgga cttcgcatgg catatcagca accggttgac gggctttaat gaaacgatga
      361 ttgtgtattt gctggcgatg gcttcgccga cgcacgccat ttctccagag ttgtattaca
      421 ccggttgggc gaaccaaaag catgcggcgg tgggttcaag tatcgacgat ccgcgtccgg
      481 gacctcgcta caccaacggg agaaaatatt tcgggatcaa gctggacgtt ggctggggaa
      541 cgggaggccc gctctttttt acgcactatt cttatatggg gtttgatccc catgcggcta
      601 ccgaccgctt cacgaactat tttgaaaata accggaacac cgcgctgatc aatcgggcct
      661 attgcatcgc caacccaaaa catttcgagg gatattcggc ggagtcatgg ggactgacgg
      721 ctagcgatgg tccggagggt tacaatccca gcgcgccgga tgaagatgac gatcatggtc
      781 tcatggcgcc gaccggagcg ttgtcttcgt ttccgtacac tccggaagcg tcgatggccg
      841 ctttgaaaca tttttatcgc gatgagggca gctggctctg gggcgtttat ggaccgcgag
      901 atgcgtttga tgcgcacggg cattgggtat cgccgattta catgggactg aaccaggctc
      961 cgattacggt gatgattgaa aattaccgga ctggcctaat ttggaagtgg ttcatgtcca
     1021 atccagagct tgaaccgatg ctcgcgaagg cgggattaca aatgcacacg catgggacta
     1081 agcgagacag cacgcaatga ttgcgtggat tgcgcaagat ttttgcttct tttgagttgt
     1141 tgttcgcgta atagttgtct cctttcgctc gccgcatcta tggtatgaat gtgctttgga
     1201 ggcttgttga gcgaaattcc ctcagtgact gtagcgggtt cggcacttca ggaactactt
     1261 cctcccctca gcgagcaaga agcggtcacg gaatcggcgc gctgcttgat gtgcttcgac
     1321 gcaccctgta cgcacgcttg tcctacgcat atagacattc ccaaattcat taagaaaata
     1381 tccaccggga atttactggg gtccgctcgc actattctgg aatctaattt gcttggggct
     1441 acctgcgcgc gggtgtgtcc ggttcaagaa ttgtgcgaag gcgcttgcgt gctcggttcg
     1501 gagcacaaac caattgcgat cggccggctg cagcggcacg cgatggatta cgtgtatgaa
     1561 cggaagattg acgttttcca cgctgggaaa tcgattggca agaaggtagc cgttgttggc
     1621 gctggtcctg cgggcttatc ttgcgcgggt gagctcagca agttgggcgt ttcggtgacg
     1681 ctgtatgaaa aacgtgagct acccggtgga ctttctactt acggcatcat cgtgctgcgc
     1741 gaaccggtgg aagttgcgct tgcggaagtg gcgatgattc aacgtctcgg ttcacggatt
     1801 gaacccggca aggaagtggg ggtcgatccc ggtgtcttgg acttgcaaaa aaattttaat
     1861 gcggtattca ttggcgttgg cctcggcaac tccccagaac tgggaatccc cggcgaagag
     1921 tacatacttg acggcctcag atatatagag gacagcaaac ttcagcccgc gcaattacag
     1981 gtcggccgca atgtaatcgt gatcggcgcc ggtaacactg cgattgactg tgcgacagtg
     2041 gctcgccgtc ttggggccga acgcgtcact atgctttatc gccgcactga gaaagaaatg
     2101 actgcatacc cgcatgaata tgcgttcgtg aaactcgaag gagtcagcgt ctcgtttcta
     2161 ttcaagtctg tcaaagttca tcatgagaat ggcgtagtga aggcgctgga gtgcgttcgc
     2221 gtcgctttgg gcgaggtgga cgcgtcgggg cgcgctgctc ccaaagagat tcctggttcg
     2281 aatgtgcttt tgcctgccga ccagattgtc aaagctatcg gccagaataa attgtcactg
     2341 gcttcgcagc ttgatctcaa aacggagagg ggttttatcc gagttgattc aaattttgaa
     2401 actagttctc cgcgaatttt tgcggggggc gattgcattc gcgcgcgcgg gtcggcttct
     2461 acggtgatgg cggtgcagga cggtaagctc gcagcccgcg cgatttacca aaggctcaaa
     2521 aatcatggct gatctcagaa tcaactttgc gggcattcgc tcgcctaatc cgttctggct
     2581 ggcgtccgcg ccgccgacca attctggcgc gcaaattcac cgtgcgttcg aagcgggctg
     2641 gggcggtgcg gtttggaaaa cgattggcgc gccggtgctg aatgtgtcca atcgctatgg
     2701 ttcgtggcgt tacggcaacg aaaaaatgct ggccataaat aacgtcgagc tgatttccga
     2761 tcggcccatg gaggttaatc ttcgggaaat tgcggaagta aagcgggcgt ggcccgatcg
     2821 cgcggtgatt gtgtccgcga tggtggagtc caagccggag tcgtggcacg acgttattcg
     2881 ccgcattcag gacactgggg ctgatggcat tgagctgaat tatggctgcc cgcacgggat
     2941 gagcgagcgc ggaatgggta gcgcggtcgg gcaagttccg gaatattgcg aagagattac
     3001 tggctgggtc atggaagtcg cgaagattcc ggtaatcgta aagttgaccc caaacatttc
     3061 tgatatcgtc atgccggcgc gcgccgcggt tgcagcgggg gcaaatgcga tttctctgat
     3121 caacaccatc aactcgatca tgggcgttga tttggatacg ttggacatta cgccgagcat
     3181 tggtggaaaa ggaggccatg gcggttacgc tggcccggcg gttaaaccgg tcgcgctgaa
     3241 tatgcttgcc gctctgggcc gtgattcggt cgtgcatgga tctggcttgc cgatctccgg
     3301 catgggcggt atttctacat ggcgggacgc cgcggaattt ctgcttctgg gggcgagcag
     3361 cctgcaagtc tgcaccgctg tcatgcacta cgggtttcgc atcatcgagg atctttgcga
     3421 tggattgtcg aactggatgg acgccaagca gctggccacc attcctgatg ttgtcggcca
     3481 tagcctggcg caggtctcgg acttcaagga tttcgatctc tcatttaaag ttgtggcgcg
     3541 cattgaccag caaaagtgta ttcgctgcga cctgtgctac gtggcttgca atgacgcggc
     3601 acaccagtgc atcgacctga ttgataaaca ggggcacgta actggtccta cggcttatga
     3661 gccatcgtct aacggcagag tcgaagccgt gtcgacgcga cccaacgtgg tggttcggga
     3721 gcctgattgt gtcggctgcc gcctgtgttt caatatttgc ccggtggaaa attgcataca
     3781 catggtggaa gtgcgccccg agcaaccgtc aatcacttgg gacgagttga ccgcgaagca
     3841 gcctgaagta gccgaagatt gggatcagat ggagcgctat cgccagaaaa tgggcatccg
     3901 catccactaa aattgcggaa ggagcaagca atggggctgc tgattcgaaa tggagagatc
     3961 gtgaccgtcg gctcgcggac acgagccgat atttacgccg agaacgaaac ggtcacgcgc
     4021 atcggcaaaa atctcgacgt gccgcatggc acagaaatca tcgatgctgc cggaaaattt
     4081 gttttcccag gtttcatcga tccgcacgtg catatctatc ttccctttat ggccactttc
     4141 gcgaaggaca cgcatgaaac cgccagcatt gctgcgctta ttggcggcac aactacctac
     4201 attgaaatgt gctgtccgaa ccggactgac gacgcattgg cgggctatga actttggaaa
     4261 tcgaaggccc gggggaagag tgcctgcgat tacgcgttcc acatgtcggt cacaaaattc
     4321 ggcgaagaaa caaaatcgca gcttcgggaa attgtggctg acggcacttc ttccttcaaa
     4381 atctttctcg cttataaaaa cttctttggc gtcgatgacg gggaaatgta tcagacactg
     4441 acgctcgcga agaagctcgg ggtgatcgtc actgcgcact gcgaaaatgc ggagctggtc
     4501 gcgcggcttc aggcgcaact catcgccgag ggcaagaccg ggccggagtg gcatgagcct
     4561 agccggcccg agtctgttga agcggaaggc accggacggt ttgcgacgtt tcttgaaaat
     4621 accggtgcga ccggatacgt cgttcacctg tcttgcgaaa gggctctcaa tgccgctctt
     4681 gctgccaaag aacgcggcgt gcaccttagc gtggaagcgg ttctccctca tttgctgctc
     4741 gacaaatcgt tcgccgagcg ccccggcgtc gacggcatga agcatgtgat gtcgccgccg
     4801 ctgcgcgcca aacaaaatca ggacgtgctt tgggatgcgc tggcacgcgg tttcgtggat
     4861 accgtcggca cggaccattg tccattcgac gtcgaacaga agctcctagg taaagatgcc
     4921 ttcacaaata ttcccaatgg cattccgggc atcgaagacc gtgtcaatct tctttattct
     4981 tatggggttc agaaagggcg catcgatctg gatcgctttg taaatattgc cagcactcgc
     5041 gcggcaaaac tgttcggatt gtttccgcgc aaaggcacga tcgcggttgg aagcgacgcc
     5101 gatctggtgg tctttgacac caactatcgt ggaatcgtga gcgccaagac gcaacacgtc
     5161 aataacgact acaacgggtt tgaaggaatg gccatcgaag ggcggcctgc agtggttacc
     5221 gtacgtggca aggtgcaggt gcgagaggga gaatttgtgg gcgaacgcgg gcgcggacgt
     5281 ttgctgaagc gcgagcctgc gaatttctga gcgaatacac catgtctctt gatcccaaac
     5341 gcaccgttgc cgaactgaaa gaacttcgct cgctgaccgg tgatgaaaac ggggcgcaac
     5401 gagtagcctg gactaataca tggttgcgcg cccgtgagtg gtttgcgaaa aaactcgccg
     5461 aacttccagt cgaacatcat tacgatgcag ccgggaataa ttgggcgaca ttagcaggcg
     5521 aatcgaaaaa atcgctgcta ctcggcgggc atcttgattc ggtgccgaac ggcggttggc
     5581 ttgacggcgc cctgaatgtt cttgccgcgc tcgcagtgct tcgccgtttc gcttcagaat
     5641 tcaatgggcg gccaccggta acgatacggc tggtggactg ggctgacgaa gaaggtgcgc
     5701 gcttcggacg cagcttgttg ggatcttcag cgtttgcggg cacgcattcc atttccgcgg
     5761 accgcgttcg caccgaccgt gacggaataa aaatggaaga agcgctgcga cgttgcggcg
     5821 ttgaaatgga tcgcttcgcc gaagcgggaa aagaacgcca ggatgccgct gcgtatctcg
     5881 agctacatat tgaacaaggc cccgttcttg aacaactcgg gctcccactg gccgtggttc
     5941 tgggcaccaa gggagtggag cgccacagca taatttttca cggacaagaa gctcactccg
     6001 gttcgacacc gatgaacgcg cgccacgacg cgcttgcagc cgccgctaaa ctcgcgctcc
     6061 agattcgcaa tatcgcgagc aagcatccag atgcggtttg caccatgggg agcctcaaaa
     6121 catttcccgg aatagtgacg gcggtggtgg gccgctgcga agctacgctg gatcaacgcg
     6181 atctcgatgc gcgcatcttg gcacagatgt ttgaagaagc gcagcgggca agccgacgct
     6241 tcgccgaaga ggaaaaatgc acggtggagt ggtcgcgcat ctggaacata gagcctgtgc
     6301 ccttccatcc ggagctgctg gatcttt
//
LOCUS       DATCBB010000064         1354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_294388, whole genome shotgun sequence.
ACCESSION   DATCBB010000064 DATCBB010000000
VERSION     DATCBB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294388"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VII37_00770"
     CDS             complement(<1..1171)
                     /locus_tag="VII37_00770"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5068277.1"
                     /translation="MSLASVMRSEQELLLPTYDRHKILFEKGRGVYLWDSLGNRYLDF
                     LSGIGVNALGHGHPAIRAVLTRQAAKLIHVSNLFFHEYQAELAKRLTKISGLDRAFFC
                     NSGTEAWEGALKLARAHARAQNNNGHKAKWRVLALENSFHGRTFGSLATTGQEKYRRP
                     FMPLMPGVNFVAFNDVEDLNRQFDGSVCAICVETIQGEGGIHPLNSEFLQAARQLSER
                     TGALLLVDEIQSGLGRTGRYFAYQHYGIKPDIVTVAKPLAAGLPLGAILTTNLAASGM
                     HPGMHGTTFGGGPLACAVAIEFLRILDGMMGHIRELGNYFHAGLDTLKSKHSAIREVR
                     GMGLMLGAELDSSETAKAVVAQLLQRGVLINRTHETVLRFLPPYIIEKKHVDQVIR"
     gene            complement(1168..>1354)
                     /locus_tag="VII37_00775"
     CDS             complement(1168..>1354)
                     /locus_tag="VII37_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068278.1"
                     /translation="EMAALVAASVIRGGMLPKLEACTQALKKGVGRVRILPAAKADIL
                     PQFYFSKLECGTEVIGA"
BASE COUNT          313 a          410 c          333 g          298 t
ORIGIN      
        1 cgcggatcac ctgatccacg tgtttcttct cgatgatgta aggcggcaaa aaacgcagca
       61 ccgtttcgtg agtgcgattg ataaggacgc ctcgctgcaa cagttgcgcg accacagctt
      121 tggctgtttc ggaagaatcc agttccgcgc caagcatcag gcccatccct cttacttccc
      181 ggattgcgga gtgcttgctt ttcagagtgt ccaatcctgc gtggaaatag ttgcccagtt
      241 cacgaatgtg ccccatcatc ccgtcaagaa tgcgcaagaa ttcgatcgcg acggcgcagg
      301 ccaatgggcc tccgccaaac gtcgtgccgt gcatccccgg atgcatgccg cttgcggcca
      361 gattggttgt aagaatcgcg cccagcggca atcccgcagc cagtggtttc gccaccgtca
      421 caatgtccgg ctttattccg taatgctgat atgcgaaata acggcctgtg cgtccgaggc
      481 cgctttgaat ctcatccacc agcagcagag ctccggtacg ctcactcaat tgccgtgccg
      541 cctgcaaaaa ctccgagttc aacggatgaa ttccaccttc accctgaatc gtctcaacac
      601 aaatggcgca aacgctgcca tcgaattggc ggtttaagtc ctccacatcg ttaaaagcaa
      661 caaaattaac cccaggcatg agaggcatga aaggacgccg gtatttttcc tgtccagtcg
      721 ttgccaacga gccgaaagta cggccatgaa atgaattttc cagggccagc acacgccact
      781 ttgccttgtg accgttatta ttttgcgctc gcgcatgcgc gcgtgcgagc ttcagcgccc
      841 cctcccaagc ctctgttccg ctgttgcaaa aaaatgcccg gtccaagccc gaaattttcg
      901 tcaagcgttt ggcaagttcg gcctgatatt cgtgaaagaa aagattcgaa acgtggatga
      961 gctttgcagc ctggcgcgtc agaacagccc taatcgcagg atgcccgtgc cccagcgcgt
     1021 tcacgccgat gccgctcaga aaatccagat acctgttccc gagcgaatcc catagataaa
     1081 cgccgcgccc cttctcgaaa agaattttgt ggcggtcgta cgtcggcaat agcaactctt
     1141 gttcggaacg cattacggaa gcgagactca tgccccaatc acctccgtgc cacattccag
     1201 cttggaaaaa taaaattgcg gcaggatgtc agctttcgcc gccggcaaaa tacggacgcg
     1261 gccgacacct ttcttgagcg cctgagtgca ggcttccagt ttcggcagca tacctcctct
     1321 aatgaccgat gcggcgacca gtgccgccat ttct
//
LOCUS       DATCBB010000065         1640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_2212, whole genome shotgun sequence.
ACCESSION   DATCBB010000065 DATCBB010000000
VERSION     DATCBB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VII37_00780"
     CDS             <1..436
                     /locus_tag="VII37_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5068279.1"
                     /translation="EYTHFLETTAGDILSRGPAFGVPSESVDGQDVSAVYQVASRLVK
                     RARDGEGPSFLMCNTYRYTGHHVGDISREYYRSKQEEHHWKTERDPLKLFAEKLIARK
                     LTDAPSLVKLEEEVRAEMKVAVDFAIAAPYPNADKVEQDVYA"
     gene            429..1436
                     /locus_tag="VII37_00785"
     CDS             429..1436
                     /locus_tag="VII37_00785"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5068280.1"
                     /translation="MPELVERAVERELTFAQAVREALAEEMRRDPTVCIIGEDVAEAG
                     TPFKVLSGLVEEFGKERVIDTPISEAGFTGVGVGAAMTGMRPVVDIMFGDFLTLTMDQ
                     MVNQAAKVHYMSGGKWKVPMVLRTTLGATRRSAAQHSQSLHAWFSHVPGLKVVLPSTP
                     YDAKGLLKTAIRDDNPVIFFEDKMMYKTKGPVPEGDYTIPFGVADVKREGSDITIVGT
                     SSMVQVALGAAALLDEIGISAEVIDPRTTWPLDEATLIASVKKTSRAIVVDEGYGRYG
                     VTAEIASVIAEGAFFNLEAPIKRMGAMHVPIPFSPPLEDVTVPTEQTVFEAARKLCGK
                     A"
     gene            1459..>1640
                     /locus_tag="VII37_00790"
     CDS             1459..>1640
                     /locus_tag="VII37_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068281.1"
                     /translation="MALKTYGAMAVDWENRIDFERLRTERLARAKALLAKSEMGALLC
                     FDMNNVRYLTATHIGT"
BASE COUNT          378 a          406 c          515 g          341 t
ORIGIN      
        1 cgaatatacg catttcctcg agactaccgc gggcgacatt ttgagccgcg ggccggcgtt
       61 tggagtgcct tcagaaagcg tggacggaca agatgtaagc gctgtgtacc aggtggctag
      121 ccgcctggtg aagcgcgcac gcgatggcga aggcccgtcc ttcttgatgt gcaacaccta
      181 tcgctacact ggccatcacg tcggcgacat cagccgcgag tattaccgct ccaagcagga
      241 ggaacatcac tggaagaccg agcgcgatcc gttgaaacta ttcgcggaaa aattaattgc
      301 gcgaaaactt actgatgcgc cgtcgctggt gaaactcgaa gaggaagtac gtgccgagat
      361 gaaggtggct gtagatttcg cgattgccgc gccatatccg aatgccgaca aagtggagca
      421 agacgtttat gcctgagcta gtggaacgcg cggtggagcg ggaattgacg tttgcgcagg
      481 cggtgcgcga agcgctcgcc gaagaaatgc gccgtgaccc tacggtgtgc atcatcggcg
      541 aagacgtagc ggaggctggc acgccattta aagtgctatc aggactggtg gaagaattcg
      601 gcaaggagcg ggtgattgat acgccgattt ccgaagctgg attcacgggc gtgggcgtgg
      661 gcgcggcgat gactggcatg cgtcccgtgg tcgacataat gttcggcgat tttttgacgt
      721 tgacgatgga tcagatggtc aatcaggccg ccaaagttca ctacatgtcc ggcgggaaat
      781 ggaaagttcc catggtgttg cggaccacat tgggtgcgac gcgacggtcg gcggcgcaac
      841 actcgcaatc attacatgcg tggtttagcc acgtgccggg cttgaaagtg gtgttgcctt
      901 cgactccgta cgacgcgaag ggtttgttga agacggcgat tcgagacgat aacccggtga
      961 ttttttttga agacaagatg atgtacaaaa cgaaagggcc agtcccggag ggggattaca
     1021 ccattccttt cggcgtcgcg gacgtcaagc gcgaaggctc ggacattact attgtgggca
     1081 ccagcagcat ggtgcaagtg gcgcttggcg cagcggccct gctggatgaa atcggcatca
     1141 gcgctgaagt gattgatcct cggacgacgt ggccgctcga tgaggcgaca ttgatcgcgt
     1201 cggtcaaaaa aacgtctcgc gcgattgtgg tcgacgaggg ctatggacgt tacggtgtga
     1261 ccgcagaaat cgcttcggtg attgccgagg gcgcattttt taatctcgaa gcgcctataa
     1321 aacgcatggg cgcgatgcac gttcctattc ctttttctcc tccgcttgaa gatgtaaccg
     1381 ttcccaccga acaaacggta ttcgaagccg cacgcaaact ttgcggcaaa gcctgaacag
     1441 cagcatagga gggatatcgt ggcgctgaaa acttacggag cgatggcggt cgattgggaa
     1501 aatcgaatcg atttcgaacg attgcgcacg gaacgcctgg cacgagccaa ggcgttactg
     1561 gcgaaatccg agatgggcgc gctgctgtgc tttgacatga acaatgtgcg ttatctcacg
     1621 gccacgcaca tcggcacctg
//
LOCUS       DATCBB010000066         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_424178, whole genome shotgun sequence.
ACCESSION   DATCBB010000066 DATCBB010000000
VERSION     DATCBB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424178"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1447
                     /gene="uraD"
                     /locus_tag="VII37_00795"
     CDS             62..1447
                     /gene="uraD"
                     /locus_tag="VII37_00795"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY5068282.1"
                     /translation="MRSHPAEYLLVTPNNLPSVLSLLAQQPGTWLPIAGGTDLMVQYA
                     AGHLATRKLVSIWNLPELRRIVILPGEIRVGAGCTYTDLIEHADVNREFPLLTTAARW
                     TGGVANQNRGTLGGNIVNASPAADSLPALLVYGAELILVSVRGERRVLYENFHTGYRK
                     TQCAADEVIQFVCLPSRFSDYLAYTRKIGARNAQAISKVCLAALGRVAGGVIEDIRIA
                     VGSVAPIPFRLTETETALRGKRIDPTLILLAKKIVADEVQPIDDIRSSARYRRAVTAN
                     LVAEFLNGFNEAGNKKSNVLTRWNSLPLLQAAVEIFPCCGSNLWARQIAAVRPLKDED
                     SLLAASDRIWTSLTEDHWMEAFAHHPRIGHSVAPSTAPVRSAQWSTEEQQKVGAAEDN
                     VLAALAEGNRAYEERFGRIFIVCAAGKSAHTILDILQRRLRNDEQTELEESAEQQRQI
                     TSLRLKKWMSE"
     gene            1444..1788
                     /gene="uraH"
                     /locus_tag="VII37_00800"
     CDS             1444..1788
                     /gene="uraH"
                     /locus_tag="VII37_00800"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY5068283.1"
                     /translation="MSRISTHVLDVSLGKPAKDVSVRLERREHSGEWAVLFSSRTGAD
                     GRCAQLLPPNETLVAGVYRLNFDTAGYFQAQDIATLYPLIEVAFQVLEGESEFHIPLL
                     LSPYGYTTYRGS"
     gene            1793..2641
                     /gene="pucL"
                     /locus_tag="VII37_00805"
     CDS             1793..2641
                     /gene="pucL"
                     /locus_tag="VII37_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEY5068284.1"
                     /translation="MIALAENKYGKSRVRLVQVKRRGDRHDFREWTVEILFTGDFESC
                     FVEGDNRNILPTDTMKNTVYSLARESSQTCLEEFAEELADFFLDNNPQLSASQVSISE
                     KTWERVMVEGKPHPTTFVQSSAELQTAEISRARDGASSVWSGLDNLIIMKTAGSEFAG
                     FLKDSLTTLPESTDRLLGTALRARWKYSAMDVSFRTLRKSIRQILLGVFAGHSSKSVQ
                     HTLYAMGEAVLQQIPEVDEIDLNMPNRHCLLVDLARFGQDNPNEIFVPVDEPHGYIEA
                     RIRRQG"
BASE COUNT          620 a          732 c          752 g          638 t
ORIGIN      
        1 gctgcaccgg ttacatgcaa atttttgaag ctgtcgcgga tgccgcccgc cggtgtaggc
       61 catgaggtcc caccctgccg aatacctgct cgtaactccc aacaatttgc cgtccgtgct
      121 atcgctgctc gctcaacagc caggcacctg gctccctatc gcgggcggca ccgatttgat
      181 ggtgcagtat gccgcgggac atcttgctac gcgaaagttg gtcagcattt ggaatttgcc
      241 ggaacttcgg cgcattgtaa ttcttcccgg cgaaatccgc gtcggagccg gatgcacgta
      301 taccgatttg atagaacatg ccgatgtaaa ccgtgaattt ccgctgttga ccaccgccgc
      361 gcgttggacc ggcggagtgg cgaatcagaa tcgtggaacg ctcggtggca atatcgtcaa
      421 cgcttcgccg gctgcggatt ctctaccggc gttgctcgtg tacggagcgg agttaattct
      481 agtttccgtt cgaggcgagc ggcgcgtact ttacgaaaac tttcacaccg gttaccgcaa
      541 aacgcagtgc gccgcagatg aagtgattca gttcgtatgc cttccgagtc ggttttcgga
      601 ttatcttgcg tatacgcgta agattggggc gcgcaatgca caggctatct cgaaagtgtg
      661 tttggcggcg ttgggccggg ttgctggagg cgtgattgaa gatatccgca tcgctgtggg
      721 aagtgttgcg ccaattccgt tccgtctgac agaaactgaa acagctctca gaggcaagag
      781 aattgacccc acacttattc tgctggcaaa gaaaatagtg gctgacgaag ttcagcccat
      841 cgacgatatt cgatcctccg ccagatacag gcgcgccgtg acagcgaatc ttgtggctga
      901 atttctgaat ggttttaacg aagccggaaa taaaaagtcg aacgtgctga cgcgttggaa
      961 ttctctgccc ctactacaag cggccgttga aattttcccg tgctgcggtt caaacctctg
     1021 ggcacgacag atagctgctg tcagaccgct aaaggatgaa gatagtctgc tggctgcgtc
     1081 cgacagaata tggacaagtc tcacggaaga tcattggatg gaagccttcg ctcatcaccc
     1141 gcgcattgga cattcggttg cgccttcaac ggctcccgtg cgctcggccc agtggtccac
     1201 cgaagagcag caaaaagttg gcgcggctga ggacaacgtg cttgccgcgc ttgcggaagg
     1261 caatcgagcc tacgaagagc gcttcggacg tatttttatt gtgtgtgccg cgggcaagtc
     1321 cgcccatacg atcctggata ttctgcagcg acgcctgcgc aacgacgaac agaccgaact
     1381 tgaggaatcg gccgagcaac agcgacaaat taccagcctg cgcttgaaaa aatggatgtc
     1441 tgaatgagcc ggatttccac gcacgtcctt gacgtttcgc tcggcaaacc cgccaaagat
     1501 gtttccgtgc ggctggagcg acgcgaacac tctggcgagt gggccgtgct attttcgtcg
     1561 cgcaccggtg ctgacggacg gtgcgcacaa ctgctgccgc caaacgaaac tcttgtcgcc
     1621 ggcgtttacc gcttgaattt cgataccgcg ggttattttc aggctcagga catcgcaact
     1681 ctctatcctc ttatagaggt cgcatttcaa gtgctcgaag gcgaatcaga gtttcatatt
     1741 ccgctgcttt tgagcccgta cgggtacacg acttatcgag ggagctgata gcgtgatcgc
     1801 tttggccgag aataaatatg gaaagtcgcg cgtccgcctg gttcaggtga agcggcgtgg
     1861 cgatcgtcac gattttcgtg aatggacggt agagattctt tttacaggag attttgaatc
     1921 ctgcttcgtc gaaggcgaca accgcaacat ccttcccacc gacaccatga aaaacacggt
     1981 gtactcgctg gcccgcgaat cttcgcagac atgcctggaa gaatttgcgg aagaattagc
     2041 ggactttttc ctggacaata atccgcaact ttccgcgtcc caggtttcta tctctgaaaa
     2101 aacctgggaa cgcgttatgg tcgaaggaaa acctcatccc acaacatttg tgcaatcgag
     2161 cgctgaactc cagaccgcgg aaatatcgcg agcgcgcgat ggcgcatcgt cggtctggtc
     2221 gggcctcgac aatctgataa ttatgaagac cgccggatcg gaatttgccg gtttcctgaa
     2281 agattcgctg actactttgc ccgaatcgac ggatcgttta ctgggcacgg cacttcgcgc
     2341 gcgctggaaa tattctgcga tggatgtttc ctttcgtact ttgcgaaaga gtatccgcca
     2401 aattctgctt ggcgtatttg cgggacattc cagcaaatcc gtgcagcaca ccttgtacgc
     2461 catgggtgaa gctgttctcc agcagattcc cgaagtagac gaaatcgatt tgaacatgcc
     2521 gaaccgccac tgtcttcttg tagatcttgc gcgctttgga caggacaatc ccaatgagat
     2581 atttgttccc gttgacgaac cgcacggcta catcgaggcg cgcattcgcc gccagggtta
     2641 agagttaatg gcaagctctg aggccatcgt ggatcgcgcc gaaaaagtaa tcgctcgatg
     2701 caagcggtta gcgtctatta ccgaaacacc gggcagcacc tg
//
LOCUS       DATCBB010000067         1750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_358933, whole genome shotgun sequence.
ACCESSION   DATCBB010000067 DATCBB010000000
VERSION     DATCBB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358933"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VII37_00810"
     CDS             <1..486
                     /locus_tag="VII37_00810"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY5068285.1"
                     /translation="RNARGVEKSAGYFLGRFFFGCANQYADAICVAGGRSGVLAVRFD
                     GEALSRAAITRMERTLTEVEKTSGISRQDVGAFATHQPNPRLVTLLAKQCNVPVGVFP
                     EVARKSGNLGSSTCGVALQVALESAARVPVAARKPIFLASLGPGLLYGGGWLRPAADV
                     A"
     gene            complement(536..1354)
                     /locus_tag="VII37_00815"
     CDS             complement(536..1354)
                     /locus_tag="VII37_00815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5068286.1"
                     /translation="MPENYLQAAIDIAREAGQILREEFARPAKISYKGDEVDLVTQAD
                     KRSEAAIVARITKLFPDHAIAAEEGGGHETAAASGYRWHVDPLDGTTNFAHGYPCFCV
                     SLALAQHDSLIAAVVYNPIYEELFAAARGQGATLNGNKITASKIATLSTSLLCTGFPT
                     HKRTDSPNLRYYGDFTMRSHGVRRDGSAALDLASVAAGRFEGFWEFGLKPWDTAAGVL
                     LVEEAGGKVTDFSGQPYRLGGLVIMATNGLIHDEMRAVAEKIGSRPSLQLQRTE"
     gene            complement(1468..>1750)
                     /locus_tag="VII37_00820"
     CDS             complement(1468..>1750)
                     /locus_tag="VII37_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068287.1"
                     /translation="IVRPVKNESGRNTAGEHAIETITDTTGGAIFFPDTPQDLDIIFN
                     RIDRELRTQYRLCYYPNPPGPANTYRSIQVKVLDNYKVRHRKTYLTGPK"
BASE COUNT          345 a          434 c          563 g          408 t
ORIGIN      
        1 cgcaatgctc gcggagttga aaaatccgcc ggctatttct tgggaagatt tttctttggg
       61 tgcgccaatc aatacgccga tgcaatttgc gtggcgggcg gcaggagcgg cgtactggca
      121 gtgagattcg atggggaggc attgtcgcgc gcggcgataa cgcgcatgga gcgcacgctg
      181 acggaggttg agaaaacgag tgggatttct cggcaggatg ttggtgcgtt tgcgacgcac
      241 caaccgaatc cacggcttgt gacgttgctg gccaagcaat gcaacgtgcc ggtgggagtt
      301 tttccggaag tagcgcgcaa gtctgggaat ctgggatcgt ccacctgcgg cgttgcgctt
      361 caagttgcgc ttgagtctgc tgcaagagta ccggttgctg cgcgcaagcc gatatttctg
      421 gcatcgctag ggccgggact tttgtatgga ggaggttggc tgaggcccgc tgcggatgtt
      481 gcgtgacctt tgacctgcgc ttcggaaatt tcagatgacg atactcacac tttcgctatt
      541 cggtacgctg tagttgcaat gatggacgag acccgatttt ttccgccacc gctcgcattt
      601 catcgtgaat aaggccgttc gtcgccatga tgaccagccc acccaggcgg tatggctgcc
      661 cagaaaaatc ggtaactttg ccgccggctt cttctactag caagacgcct gcagcggtat
      721 cccaaggctt caatccaaat tcccagaaac cttcgaagcg gccagctgcg acgctggcta
      781 agtcaagcgc cgcggaacca tctcgccgga ctccatgcga acgcatggtg aaatcgccgt
      841 agtacctcag attggggctg tcggtgcgct tgtgcgtcgg gaagccggta caaagcaggc
      901 tggtagaaag cgtggcaatt ttcgaggcag tgattttatt gccattgagg gttgcgcctt
      961 ggccgcgagc ggcagcgaag agttcctcat agatggggtt gtaaaccacc gcggcgatca
     1021 gcgaatcatg ttgcgcgaga gccagagaga cgcagaagca gggatagccg tgcgcgaaat
     1081 tggtggtgcc atcgagcgga tcgacgtgcc atcggtagcc ggaagcggcg gccgtctcgt
     1141 ggccaccgcc ttcttcggcg gcgatggcat ggtcggggaa tagctttgtg atgcgcgcga
     1201 cgatagcggc ttccgagcgc ttgtcggctt gggtgaccag gtcgacttcg tctcctttgt
     1261 aggaaatttt tgcagggcgc gcaaattctt cgcggaggat ttggccggct tcgcgggcaa
     1321 tgtcgatagc agcttgcaga taattttcag gcatcaagaa ttttgaaaga accccgaaga
     1381 taatttcagc attgctagtc tatcaggcgg ctactctgcg agcgtgattt tagccagcgc
     1441 taccttatgt gccgccaact tttccgccta cttcggaccg gtgaggtacg tttttcggtg
     1501 gcgaaccttg taattgtcga gcactttgac ctggatgctg cggtaggtgt tggcggggcc
     1561 cggcggattg ggataatagc aaagccgata ttgggtgcgc agctcgcgat caatgcgatt
     1621 gaagatgatg tcgaggtcct gaggagtatc ggggaagaaa atggcgccac cggtggtgtc
     1681 ggtgatggtt tcaatggcgt gctcgcctgc ggtgttgcgg ccgctttcgt tcttcaccgg
     1741 gcgcacgatg
//
LOCUS       DATCBB010000068         1876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_359002, whole genome shotgun sequence.
ACCESSION   DATCBB010000068 DATCBB010000000
VERSION     DATCBB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359002"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VII37_00825"
     CDS             complement(<1..212)
                     /locus_tag="VII37_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5068288.1"
                     /translation="MANPLQLEVNGKRYSVKYPGDTPLLYVLRDELGLTGTKYGCGEG
                     QCGACTVLLGGAPRRSCQIPVSAAAGR"
     gene            complement(228..872)
                     /locus_tag="VII37_00830"
     CDS             complement(228..872)
                     /locus_tag="VII37_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5068289.1"
                     /translation="MEKKRFTALLLAALTLFALLCIAMRSAAQQAPAGQAPGRKTSSA
                     SAQKPAKFKVPDNPSPHPAPEQPIPYSHKQHLAFGLKCQECHVNPEPGKLMTFPDTAK
                     CMQCHVTIAKDKPSIQKIAQYAASNQQIPWVRVYKVIPGVSWTHRAHLAAGVKCETCH
                     GEVSQISAMSEVTSVVTMYTCLTCHEMNHAKTGCETCHVRPSFEHSSVADVRKN"
     gene            complement(932..>1876)
                     /locus_tag="VII37_00835"
     CDS             complement(932..>1876)
                     /locus_tag="VII37_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5068290.1"
                     /translation="YATARQQHSGRPIPKGGSGQLTNSLAAFIEAHNGVLLTNKWVER
                     LIIENGKCVGVECSDGSSYRADKAVVSTIHIKHLVDMAPRELWGQDFVDGVNTWQAET
                     AMFVTHYATTEAPKYKVAGGTISPCEAGVLASSERALRFGYDDASGVVNLKDPPLQII
                     CCSVADPTRAPAGIHSFKVVGWQPYQLKEGPQHWDNIKKEVSDANLKYLRRFAPNLTD
                     DKILAHFIESPLDMERMNPHFWHGSAHAGAQTAAQTGPMRPMPGWAHHNMPIPGLYQT
                     GATTHPGGSVTAGPGRNCATVMLKDFGTSIEEVVNKKA"
BASE COUNT          383 a          534 c          498 g          461 t
ORIGIN      
        1 cggccggccg ccgcactcac aggaatctgg caggagcggc gtggcgcgcc accaagcagc
       61 accgtgcatg cgccgcactg cccttcaccg catccatatt tggtgccagt aagtcccaat
      121 tcatcgcgaa gtacgtaaag caatggcgta tcgcccgggt acttcaccga atagcgtttg
      181 ccgtttactt ccagctgtaa gggattagcc ataacttgtc cccgatttta atttttgcgc
      241 acatccgcaa cggaggaatg ctcgaaactt gggcgcacgt ggcacgtttc gcacccggtc
      301 ttcgcatgat tcatctcgtg gcatgtcaaa caggtataca tagttactac gctggtaacc
      361 tctgacatgg ccgaaatctg cgacacctct ccgtggcatg tctcacattt tacgcctgcc
      421 gcaagatgag cgcgatgggt ccagcttact cccgggatca ctttgtaaac gcgcacccac
      481 gggatctgtt ggttcgatgc cgcgtactga gcgatctttt gtatggaggg tttatccttc
      541 gcgatggtga catggcactg catgcacttg gcggtgtcag gaaaagtcat gagcttgccc
      601 ggctcgggat tgacgtgaca ctcctggcac ttcaaaccga aagcgagatg ttgcttgtga
      661 ctatacggga tgggttgttc tggagcgggg tgcggactcg gattgtccgg caccttaaat
      721 tttgcgggct tctgagcgct ggcgctggag gtcttgcgcc ctggcgcttg gcctgcgggc
      781 gcctgctgcg cggcgctacg catagcaata caaagcagcg caaatagtgt caaggcagcg
      841 agcaataacg ctgtgaatct ttttttctcc atgaacttcg actcccctat agatgaactg
      901 tccgtgcttt ttgcgctgcc gtattccact actaagcttt cttgttcacc acttcttcaa
      961 tactcgtgcc gaaatccttg agcataacgg tggcgcaatt tcttcccggg cccgcggtca
     1021 ccgagccccc tggatgtgta gtagcgccag tctgatagag accaggaatg ggcatattgt
     1081 gatgcgccca tccgggcatg ggcctcatag gcccggtctg cgccgccgtt tgcgcgcctg
     1141 catgggcact cccgtgccag aaatgcggat tcatgcgctc catatccaag ggactttcta
     1201 tgaaatgcgc gagaattttg tcgtcagtta aattcggggc gaatcggcgc aggtatttca
     1261 gattggcatc ggacacttct tttttgatgt tgtcccagtg ctgcggccct tcctttaact
     1321 ggtaaggctg ccagcccacc accttaaagc tatgaattcc cgcgggcgca cgcgtggggt
     1381 ccgcaacgct gcaacaaatt atttgcaacg gtggatcctt aagatttact acgccgctgg
     1441 catcgtcgta gccgaagcgt agggcgcgct ctgaactagc cagcaccccc gcttcacacg
     1501 gcgatattgt gccgcccgca accttatatt taggagcttc ggtggtggca tagtgcgtga
     1561 cgaacatggc cgtttctgcc tgccatgtat tgacgccatc tacgaaatcc tggccccaca
     1621 attcccgtgg cgccatgtcc accaaatgct tgatgtgaat cgtggacact accgccttat
     1681 ccgcgcgata ggaactgcca tcgctgcatt ccacgccgac gcattttcca ttttcaataa
     1741 tcaatcgctc aacccacttg ttggtcagca acacaccgtt gtgcgcttcg ataaatgcag
     1801 cgagggaatt agttaactgc cctgaaccac cctttggaat tggccggccg gagtgttgct
     1861 gacgagcagt ggcata
//
LOCUS       DATCBB010000069         1155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_34888, whole genome shotgun sequence.
ACCESSION   DATCBB010000069 DATCBB010000000
VERSION     DATCBB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34888"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VII37_00840"
     CDS             complement(<1..561)
                     /locus_tag="VII37_00840"
                     /inference="COORDINATES: protein motif:HMM:NF004855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5068291.1"
                     /translation="MSGSPIRVPPTSAPHTSAPNVLQPPKFATAAEERLHRRRMLAAA
                     FRLFSRFGFDEGVAGHITARDPERLDHFWVNPFGMHFGQIRASDLILVNHHGQVVEGK
                     YPVNGAAFAIHSQVHAARPDVVAAAHAHSMYGKSWATLGRLLDPITQDACAFYEDHGL
                     FADFTGVVYETSEGERIAKALGPHKAV"
     gene            complement(701..>1155)
                     /locus_tag="VII37_00845"
     CDS             complement(701..>1155)
                     /locus_tag="VII37_00845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5068292.1"
                     /translation="AEALTVAHRQGIVHRDLKPGNIMLTRGGAKLMDFGLAKSAGAGV
                     PAGTSSAPLLSAAQTMSEASPFSPLTTAGAMIGTIQYMSPEQIQGKEADARSDLFALG
                     AVLYEMITGARPLEFFAERTGAQNYSSAAAANSGLNTFILAACLQIRH"
BASE COUNT          247 a          337 c          326 g          245 t
ORIGIN      
        1 cacggctttg tgcggaccaa gcgctttcgc gatccgctcg ccctcggacg tctcatacac
       61 cacaccggta aagtccgcga acaatccatg atcttcataa aacgcgcaag cgtcctgcgt
      121 gatgggatcg agcagccgcc cgagtgtcgc ccaggatttg ccgtacatcg aatgcgcgtg
      181 cgcagcggcc accacgtcag ggcgtgcggc gtgcacttgc gagtgaatcg caaacgctgc
      241 tccatttacc ggatatttgc cctcaacgac ctggccatga tgattcacca gaatcaagtc
      301 cgaagcgcgg atttggccga agtgcatccc gaacggattg acccagaaat ggtctagtcg
      361 ttccggatcg cgcgccgtga tgtgaccagc tactccttca tcgaatccga agcgcgaaaa
      421 cagccgaaac gcagcagcaa gcatgcgcct gcgatgcaag cgctcctcgg ccgcggtggc
      481 aaatttaggc ggttgcaaaa cgttcggcgc ggaagtgtgc ggtgcgcttg tgggcggtac
      541 gcgtatgggc gagcctgaca tgtttccctc cagtgcgtag aaactaacag tttagctacc
      601 ttgcgacaaa tgcggcgtgt tgctcgccgg atagtctttt gcctgtcacg cggaggctca
      661 cataacgata gtcctcttgt gctaaagggg caggatgtgc tcaatgccgt atttgtagac
      721 aggcagccag aatgaaggta ttaaggccag aatttgcagc ggcggcgcta ctgtaatttt
      781 gcgccccagt tctttcagcg aagaattcca acggtcgtgc gccggtgatc atttcataca
      841 agacggcccc caacgcaaag aggtccgatc ttgcatccgc ctctttgcct tgaatttgct
      901 ccggcgacat gtactggatg gttccgatca tggcgccggc ggtcgtcaga ggagagaacg
      961 gactagcttc gctcatcgtc tgagcggccg acaaaagagg tgcgctcgat gttcccgctg
     1021 gcacgccggc cccagctgac ttggccaagc caaaatccat cagctttgcg ccgcctcttg
     1081 tcagcatgat gttccctggc ttcaaatcac ggtggacaat cccttgacgg tgcgcgactg
     1141 taagcgcctc cgcaa
//
LOCUS       DATCBB010000070         1299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_391673, whole genome shotgun sequence.
ACCESSION   DATCBB010000070 DATCBB010000000
VERSION     DATCBB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391673"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VII37_00850"
     CDS             complement(<1..516)
                     /locus_tag="VII37_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY5068293.1"
                     /translation="MAQQFRQEKDSIGIKEIPADVYYGVQTARAVENYPISGMRANPT
                     LIRAIGMVKYAAAEANKELGLVDDKRADAIMQAAKEVVDGKWDASFVVDVFQAGAGVS
                     FHMNSNEVIANRATELMGGKLGDYTVHPNDHVNYGQSTNDVFPTAMRVATLLELEKLY
                     PVLDSLAAAFEA"
     gene            714..1055
                     /locus_tag="VII37_00855"
     CDS             714..1055
                     /locus_tag="VII37_00855"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEY5068294.1"
                     /translation="MFEGIRSILSPGPKRSEADEKLDEALIHATSLDHYFFFSDQSNA
                     DQAAERLQDRGWTIESVSLNAELEKVQLQARQPGPIENLNTLQTDLDLFADEFNGEYD
                     GWQVPGVTENI"
     gene            complement(1052..>1299)
                     /locus_tag="VII37_00860"
     CDS             complement(1052..>1299)
                     /locus_tag="VII37_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068295.1"
                     /translation="VYARVLRVFVVGHDGDCKAKPWRATASEVAEMLVQWRRAAGGAA
                     AKDAGAKDLGANDFRRKRFSAQTIFGAKDLRCERSSA"
BASE COUNT          298 a          379 c          338 g          284 t
ORIGIN      
        1 agcttcaaac gccgcggcca gcgaatcgag caccgggtaa agtttttcca actccagcag
       61 cgtagccacc cgcatggcgg tggggaagac gtcattcgtg gattgtccgt agttcacgtg
      121 gtcattggga tggaccgtat agtcacccag tttgccgccc atcagttcag tagctcggtt
      181 ggcgataacc tcattcgaat tcatgtggaa gctcacgcca gcgccagcct gaaacacgtc
      241 gaccacaaac gacgcgtccc acttgccatc cacaacttct tttgctgcct gcatgatcgc
      301 atcggcgcgt ttgtcatcga ccaggccaag ttccttgttg gcctcggcag cggcgtattt
      361 caccatgccg atagcgcgaa tcagcgtggg attggcgcgc atgccggaga tgggataatt
      421 ttccacggcg cgcgcggtct gcacaccgta ataaacgtcg gcgggaattt ctttgatgcc
      481 gatagaatct ttttcctggc gaaattgctg ggccatattt gtccctcttt ataagtgcgg
      541 aatgcgtatg cgttcgcgga gcaccgcaaa actagtgatt ataacttgag cgtgggaacg
      601 ttctgaaatg cagcagacgc cgtgtgtcaa cgaattcggc agcgccagcc gatgtcctgc
      661 agtaacgccc gcagtagcgc ccgcagtcgc cgttgtatga ttgaaaaact accatgtttg
      721 aaggcattcg atccatcctg agcccaggcc caaaacgttc tgaggctgac gagaagcttg
      781 acgaagcgct gatccacgcc acttcgctcg atcattactt ctttttctct gaccagtcca
      841 atgctgacca ggctgcagag cgcctgcaag atcgcggctg gacgattgaa tcggtcagcc
      901 tcaacgccga gttggagaag gtgcaactgc aagcgcggca gccgggacct atagaaaatc
      961 tcaatacatt gcagaccgac cttgacctct ttgccgacga gttcaacggc gaatacgatg
     1021 gctggcaggt cccaggcgtg accgaaaata tttaagccga agatctttcg caccgaagat
     1081 ctttcgcacc gaaaatcgtt tgcgccgaaa atcgtttgcg ccgaaaatcg tttgcgccga
     1141 gatctttcgc gccagcatct ttagccgccg caccgcccgc cgcccgccgc cactgcacca
     1201 gcatctcagc aacttcgctc gctgtcgcgc gccatggctt ggctttacaa tctccatcat
     1261 ggccaacaac aaagacgcga agaacacggg cgtaaacaa
//
LOCUS       DATCBB010000071         1035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_326659, whole genome shotgun sequence.
ACCESSION   DATCBB010000071 DATCBB010000000
VERSION     DATCBB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326659"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VII37_00865"
     CDS             complement(<1..868)
                     /locus_tag="VII37_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068296.1"
                     /translation="MKRIFRTRTAVLFVATMSSICSSQSATDVRLTAHAVRLKGQNPF
                     SLMLPADFTIVPAAEGLKRVRFMARSPDGRIFVTTMHDLSDNSEGAVYILDGFDEEHG
                     RFARVIPYLTNLRNPNSMAFHQDAQGRDWFYLALTDRLVRYKFTRGEERPVSQPEVLA
                     TYPAYGLSYKYGGWHLTRTIAFGQGSSNLLYVTVGSSCNACDEKEEVRATLSVMDEDG
                     KNQRIVARGLRNAVGMRFVGGALFLTNMGADHLGDDAPDDPLFSVNPRALQDGGLDYG
                     WPHCYYSGGKAKA"
BASE COUNT          250 a          320 c          242 g          223 t
ORIGIN      
        1 ccgccttggc ttttcccccc gagtaatagc aatgcggcca accgtaatcc agtccgccat
       61 cctgcagcgc tcgtggattc acagaaaaca gtggatcgtc aggcgcgtcg tcgcccaaat
      121 ggtccgcccc catattcgtc agaaacaacg cgccgcccac aaaacgcatt cctaccgcat
      181 tgcgcaagcc tcttgcgacg atgcgttgat tcttcccgtc ctcgtccatc accgataacg
      241 ttgcgcgaac ctcttctttt tcatcgcagg cattgcagct actgcccaca gttacgtaca
      301 acaaattgct actgccttgc ccaaacgcga tcgtgcgcgt caggtgccat ccgccatatt
      361 tgtagctcag cccataagcc ggataagtag ccagcacttc cggctgcgag acaggccgct
      421 cttcgccacg cgtgaattta tatcgcacca gccggtcggt aagcgccagg taaaaccaat
      481 ctcggccctg cgcgtcctga tgaaaggcca tgctgtttgg attgcgcaaa ttggtaaggt
      541 acggaatcac gcgcgcgaac ctgccgtgct cctcatcaaa gccgtccaga atgtagactg
      601 ctccctcgga gttgtccgaa agatcgtgca tggtggttac aaaaatccgt ccgtccggcg
      661 atctagccat aaaccgcacg cgcttcaatc cctcggcggc gggcacaatc gtaaagtccg
      721 caggcagcat caacgaaaac ggattctgcc ccttcaaacg cactgcgtgc gcagtcaacc
      781 tcacatctgt tgcagactgg gaagaacaaa tcgacgacat cgtagcgaca aacaaaaccg
      841 ccgtgcgcgt gcggaagatc ctcttcatgg acaaaatagt gccatcaaat ccgcgagctg
      901 ttgaggtaaa actactgtcg agataagact ggctggtcga ataaaattgc aacgatactt
      961 ttaatttttc ttgttccggt cagcctgatc ctgaaccctc ttcatgtgcc gcgacaaaac
     1021 caggtccgct acgtt
//
LOCUS       DATCBB010000072         1215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_229226, whole genome shotgun sequence.
ACCESSION   DATCBB010000072 DATCBB010000000
VERSION     DATCBB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229226"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..282
                     /locus_tag="VII37_00870"
     tRNA            196..282
                     /locus_tag="VII37_00870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:230..232,aa:Leu,seq:tag)
     gene            351..1190
                     /locus_tag="VII37_00875"
     CDS             351..1190
                     /locus_tag="VII37_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEY5068297.1"
                     /translation="MILYEQDNEVVIIRQTDHALLSGFFAREWGNELFSQPRPFESFR
                     LAAAEHDNGWQEWELLPKVDPATFLPYSFMSIPTEAHIALYQNGLERILKVDHYAALL
                     VSMHCALLYDRARATMPGYSAKYVRSSEAHQVADFVQRLRLQQLRLKVDLRTNPATSA
                     FTEEKFLQANLQRLEALDRLSLYFCLPTLGDSTIEGVPVDDQGSEADWELRREGETIT
                     LAPYPFRREPLSVSILARRVPKRRYADDREFQNTLARAPYSPVKFTLSRGKASIQSLL
                     AGA"
BASE COUNT          265 a          351 c          346 g          253 t
ORIGIN      
        1 ggtccgtggg acgtgagtcc gcgaaccgtc cttagttcat atcgcgaaaa cgagcagcag
       61 ggaagtaaaa agctccagtt ctgggcggcg tgcagccatt ccttagcaag gaattggcag
      121 cacatcacct cagcacaaaa aatgaaccgt tcatccacaa ttcaccggca tgttaaactt
      181 tcatttgcaa gctctgcggg aatggcggaa ctggcagacg cagcaggctt aggacctgcc
      241 gggcgcaagc tcttgggggt tcaaatccct cttcccgcac cattctttgc ggtatcatca
      301 agcccacgta atcagatgct acagcggaat tccaccgtca tgaacgcgcg atgattctct
      361 acgaacaaga caatgaagtc gtgatcatcc ggcaaacgga tcacgcgctg ctctcgggat
      421 tttttgcgcg cgaatgggga aacgaactgt tttcgcagcc gcggccgttt gaatcttttc
      481 gcctggcggc cgccgagcac gataacggct ggcaggagtg ggaactcctg ccgaaagtcg
      541 atccggccac ttttctgcct tacagcttca tgtcgattcc caccgaagcg cacatcgcgc
      601 tgtaccagaa cggactggaa cgcatcctca aggtggacca ctacgcggct ctgctggtga
      661 gcatgcattg cgcgctgctt tacgaccgcg cgcgcgcgac gatgccgggc tattcggcga
      721 aatatgtgcg ttcgagtgaa gcgcaccagg tcgccgattt tgtgcagcgc ctgaggctgc
      781 aacagttgcg gctgaaagtg gatctgcgca ccaatccagc gacttcggct ttcaccgagg
      841 aaaagttttt gcaggcgaac ctgcagcgac tcgaagcgct ggatagactc tcgctctatt
      901 tctgcctgcc gactctgggc gactccacca tcgagggtgt tccagtcgat gaccagggta
      961 gtgaagcgga ctgggaactt cggcgggagg gcgaaacgat tacgctggcc ccgtatccct
     1021 tccggcgtga accgttgtcg gtgagtattc tggcacgccg agtgccgaag cgccgttacg
     1081 cggacgaccg tgaatttcaa aatacactgg cgcgcgcgcc ctattctccc gtgaaattca
     1141 cgttgagccg gggcaaggct tccatccaat cgctgctcgc aggagcatga tcaactgagc
     1201 gcgcggccaa ccgtt
//
LOCUS       DATCBB010000073         1073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_229258, whole genome shotgun sequence.
ACCESSION   DATCBB010000073 DATCBB010000000
VERSION     DATCBB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229258"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..955
                     /locus_tag="VII37_00880"
     CDS             206..955
                     /locus_tag="VII37_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068298.1"
                     /translation="MGTAVATSTVKVRAGVPGRKYDHVFFSAMALLLLGSVFLGFAHS
                     YYLAGMFHAPLPSAIIHVHGALFSSWILFLLAQTTLVAAGRTDVHRRIGIAGFLLACL
                     MVIVGVWAGTNALARNFAPPGVDAKTFYVIPITDMLVFAVLVFFAYRARFDSAAHKRI
                     IIIATVALMIAAIARWPFPLLLGNPLAAALVSYLFLLMLMAYDLWSTHKIHRATIWAS
                     AFLIFVQQVRFPIAQTAAWHAFATWAQDLKL"
BASE COUNT          199 a          256 c          325 g          293 t
ORIGIN      
        1 cctgcgatgc agcaggtggt tggcgtggat tgcgaggtgg agttttccgc catgtccgaa
       61 cctcggcaag agtttgccac agatttaaac gtgccgggtt gcaacgaacg ctggctcctg
      121 gagagggtga atcgggtata caatgcaggc aatctcttct gaagttccca gttagttctg
      181 aactattgcg caatctggag acacgatggg tacggctgta gcaactagca cagtaaaggt
      241 gcgtgccgga gttccggggc gtaaatacga ccatgtgttt ttttcggcga tggcgttatt
      301 gcttctggga tcggtgtttt tgggctttgc gcatagctat tacctggcgg ggatgttcca
      361 tgcgccgttg ccgagcgcga ttattcatgt tcatggagca ctattttcga gttggatttt
      421 gtttctgctg gcgcagacta cactcgtcgc ggcgggacga acagatgttc accggcgcat
      481 tggaatagct ggatttttgt tggcatgcct gatggtgatt gttggagtgt gggcgggtac
      541 caatgcgttg gcacggaact tcgctcctcc aggcgtcgat gcgaaaacgt tctatgtgat
      601 cccgattacg gacatgctgg tttttgcggt actggtgttt tttgcctacc gtgcgcggtt
      661 tgactccgcg gcacacaaac gaattattat aatcgcgaca gtagcgttga tgatcgcggc
      721 gatcgcgcgt tggccatttc cgctgctgct cggcaaccca ttggcggcgg cgctagtgtc
      781 ctacctattt ctgttaatgc tgatggccta tgatctgtgg tccacccaca agattcaccg
      841 ggcgacgatt tgggcgagcg cgtttttgat atttgtgcag caggtgcgat ttccgattgc
      901 gcagacggca gcgtggcacg cctttgcaac ttgggcgcaa gacttgaaac tctgagcggt
      961 ccaggcgcgc agaacgcccc gccggcttaa ggttcctttc taccgaacag cttggttccc
     1021 taaatagttt gaggtttagt tttgcccggc atgcggtcaa gttggccgcg cca
//
LOCUS       DATCBB010000074         1696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_229277, whole genome shotgun sequence.
ACCESSION   DATCBB010000074 DATCBB010000000
VERSION     DATCBB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229277"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1258)
                     /locus_tag="VII37_00885"
     CDS             complement(110..1258)
                     /locus_tag="VII37_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5068299.1"
                     /translation="MDFGLTEEQKQLRRSVREFAEGEIAPHVMEWDEVSHFPLEIMPK
                     LAEMGLLGVIFPEELGGAGLGYIEYAIAIEELSRVDGSVGIIVAAHNSLCSNHIFKFG
                     NDAQKKKYLVPLAQGKKIGAWSLTEPEAGSDAGGTRTTAVREGNFWVLNGAKTFTTNG
                     HYADICVAMAKTDKSKGSHGISAFILEKGMSGFKAGKKENKLGLRASDTSEVIFTDCR
                     VPQENLLGPEGEGFTGSLKILDGGRISIAALALGMAQGALDAATKYAKQRKQFGQAIS
                     EFQAIQFKLADMATDVEAARLLVYQAAWLADKKDVRFTRESSMAKLFASEVAVRVANE
                     CVQIHGGYGFIKDYPAEKFYRDVKLCTIGEGTSEIQKLIIARQLLGKK"
     gene            complement(1271..>1696)
                     /locus_tag="VII37_00890"
     CDS             complement(1271..>1696)
                     /locus_tag="VII37_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEY5068300.1"
                     /translation="VKPEEYNKAWWGLVRKYQGVAPPMERTEADFDPGAKYHVPGNVP
                     YMRYFLARILQFQFHRALCREAGYSGPLHRCSIYGNKAAGDRLNKMLSMGASKPWPDA
                     LEVLTGEKQLDASALADYFAPLKTWLDEQNKKNNYAVGW"
BASE COUNT          335 a          554 c          382 g          425 t
ORIGIN      
        1 cgcgacagcg cccgcacgtc cccggcacgc agctcttccg cccatttttc aactctcggt
       61 gccatttttt cctttggagc ttttcacaaa acgccgcgag ccttcgatat tatttcttcc
      121 ctagcagttg ccgggcaatt atcagcttct ggatttcgct tgtaccttcc ccaatggtgc
      181 acagcttcac gtcacgataa aatttttccg ccggataatc cttgatgaat ccgtagccgc
      241 cgtgaatttg cacgcactcg ttggccacac gcactgcaac ttcgctggca aataatttcg
      301 ccatgctgga ttcgcgcgtg aagcgcacat cttttttgtc cgccaaccaa gccgcctgat
      361 acaccagcaa tcgcgcagct tccacatccg tagccatatc cgccagcttg aactggatcg
      421 cttgaaactc gctgattgcc tgcccaaatt gtttacgctg cttggcgtac ttcgttgccg
      481 cgtccagcgc tccttgcgcc attcccaagg ccaaggccgc aattgaaatc cgcccgccgt
      541 cgaggatctt caggctaccg gtgaagccct cgccctcggg tcccagcagg ttttcctgcg
      601 gcacgcggca atccgtaaaa attacttccg aggtgtcact cgcgcgcagt cccaatttgt
      661 tttctttttt tccggcttta aacccggaca tgcccttctc taaaatgaac gcgctgatgc
      721 cgtgcgaccc tttggacttg tcggtcttgg ccatggccac gcaaatgtcc gcatagtgcc
      781 cgttggtggt aaaagtcttg gcgccgttca aaacccagaa atttccttcg cgcaccgccg
      841 tggttcgagt ccctccggca tctgaaccgg cttccggctc cgtcagtgac caagccccga
      901 ttttcttgcc ttgcgccagg ggaaccagat actttttctt ctgcgcgtca ttcccaaatt
      961 taaaaatgtg attgctgcac agcgaattgt gcgccgcgac aatgatcccc accgacccat
     1021 ccacgcgcga caattcctca atcgcgatgg catattcgat gtaccccagc cccgcgcctc
     1081 caagctcttc gggaaaaatc accccaagca aacccatctc cgccaacttg ggcatgattt
     1141 ccaggggaaa gtgcgacact tcatcccatt ccatgacgtg cggcgcgatt tcgccctcgg
     1201 caaattcacg cacgctccgc cgcagctgtt tttgttcttc agttaatccg aaatccacgc
     1261 gttctcctgc ttaccatccc accgcataat tgtttttctt gttttgttca tccagccagg
     1321 tctttagcgg ggcgaaataa tcggccagcg cgctggcgtc cagttgcttc tccccggtca
     1381 gaacttccag cgcgtccggc cagggcttgc ttgcgcccat cgagagcatc ttgttcaatc
     1441 tgtcgcccgc cgccttgttg ccataaatcg agcaacgatg cagcggtcct gagtatcccg
     1501 cctcgcggca aagcgctcgg tgaaactgga attgcaaaat ccgtgccaga aaataacgca
     1561 tataaggcac attgccgggc acgtgatatt ttgcgcccgg atcaaagtcc gcttccgtac
     1621 gctccatcgg aggagcgacc ccttggtatt tccgcaccaa tccccaccac gctttgttgt
     1681 attcctcggg cttcac
//
LOCUS       DATCBB010000075         2539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_164719, whole genome shotgun sequence.
ACCESSION   DATCBB010000075 DATCBB010000000
VERSION     DATCBB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164719"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VII37_00895"
     CDS             complement(<1..297)
                     /locus_tag="VII37_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY5068301.1"
                     /translation="MKTNEVDLDHSMPTGEPVSFIITANPDGSVAYRNGVRAVGTTRL
                     GLSCGDFAGQLVLGDSAVVDNAWRGKILDLIVYERELTPSEIASKYADWNDTQAV"
     gene            827..1597
                     /locus_tag="VII37_00900"
     CDS             827..1597
                     /locus_tag="VII37_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068302.1"
                     /translation="MLLIDCLQATGYSCALALGVGDMQYLRSVVAAFLVIAMGSTPGW
                     GATGPAFGTVVAAEGANVGGGSLSAGTTVFGGDRLFTVSTGSVQLRAGAARLLLSGDS
                     AAVLARDEVSPAASLTRGTAIFSTATSKAFVMHVGSMVIRPGTDNPTVAQVTVVGPKQ
                     LMVRSTRGSVSVAVDDDVRVIPEGMAYRIVLDPSDSELASADAADQDPQGVGARSRGG
                     RPRRAGRNRFVWFVIGAGAVITYFALSEAMESPDRPNH"
     gene            1929..2447
                     /locus_tag="VII37_00905"
     CDS             1929..2447
                     /locus_tag="VII37_00905"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY5068303.1"
                     /translation="MRRLPHSLEHAHIFSARIYKQWIMRCVDVPRDVSKSLRQLAGDN
                     RKHIPVVGQVEGLPLKSTLSPRGGGSYRLHLHSNIWRKLRIDEGATVEVMLLLDGEPR
                     DPVLPHELAGGLADAPRALATFNSLTPAFRRQIVRYVEQVKHASTREKRIRLIVKRML
                     ERAAKQKKKKTA"
BASE COUNT          548 a          703 c          687 g          601 t
ORIGIN      
        1 aaccgcttga gtgtcgttcc agtcggcgta tttgctcgcg atttcgctgg gggtcagttc
       61 acgctcatag acaatcaagt ccagaatctt gccgcgccag gcgttgtcca ctaccgccga
      121 atccccgagc accaattgac cagcaaagtc cccgcaagag agtcccagtc gagttgtccc
      181 caccgcccgt acgccattac ggtaagcgac gcttccatcg ggatttgcag taataatgaa
      241 ggagacgggt tcccccgttg gcatggagtg gtcaaggtcg acttcattgg ttttcaagtg
      301 gcttttgacg tcgcggtagt cttttctgat taaaagcgta tcgcgccatt gcattaacct
      361 gaattgagat ggattttcgg gcgtataaaa ttctagtggc gtgcccccgc taggacccgg
      421 ttgcagtcga attgcgagag tacagaagga gtcccgattc agcccagagg cctttagtga
      481 gtccctactg aaaagcactc cgtgatcgcc aaattggagt ccgccgccgg cacgccacgc
      541 cacttgattt cttggaaatg gattgaaggg ccacaaaccc gctgtaaata atatgcttac
      601 gaccaatatg cacgcgagtg gcagcgccag gtctgcggcg gataagctct tttgtttgct
      661 catctgcggg ttacgggact tttgaggcca tgcaattcag tatacgacgc ttggcgcaat
      721 cattggctcg ctgcgttccg ctgatctcat tccaactata ggacaacaaa gtattaaggc
      781 acgccgatct caaaaagcat ccggccaaat aaaatagttt tgccggatgt tgctgattga
      841 ctgtttgcaa gcgactgggt actcttgtgc cctggccctg ggagtcggcg acatgcaata
      901 tttgcgctct gtggttgcag cttttctggt catagcgatg ggcagcactc ctggttgggg
      961 tgcaactggg cctgcgttcg gtaccgtcgt ggccgctgag ggcgccaatg taggcggcgg
     1021 cagtttgtcg gctggcacca ccgtcttcgg tggcgataga ttatttaccg ttagcaccgg
     1081 cagtgtgcag cttcgcgcag gggcagcccg ccttcttctt tccggcgaca gcgccgcggt
     1141 tttggcgagg gatgaagtct ctcccgcagc cagcttaact cgcggaaccg ccatcttttc
     1201 gaccgccacc tccaaagcat tcgtaatgca tgtgggcagc atggtgattc gtccgggaac
     1261 ggacaatccc accgttgcgc aggttaccgt agtcggcccc aaacagctta tggtgagaag
     1321 tacgcgcggc tccgtttcgg tcgcggtgga tgatgatgtc cgcgttattc cagaaggcat
     1381 ggcctaccgc atcgtactgg atccgtcgga ttcggaactc gcttccgctg atgccgctga
     1441 tcaagacccg caaggcgttg gtgcccggag ccgcggcggg cggccccgaa gagctggccg
     1501 taaccgcttt gtctggtttg tcattggagc cggcgcagtg atcacgtact ttgcgttgtc
     1561 ggaagccatg gaaagtcccg atcgccccaa ccattagttg agattcgtgt agcgaaagcc
     1621 gccatgccca tctacgaata catttgcgac gattgtgaaa cgccgttcga acgaattgtc
     1681 ttaaacaaaa accaggaaat ttcctgccca aagtgtgctg gaaagaaaaa taccattcaa
     1741 ctttccgtat ttagcgcgcc gaacgggaac ggaaatggcg cttccgcgac gtcatccgcg
     1801 caaatgcctt ccggtggctc ttgttgtggc ggcggttgcg gttgccattg aatctcaaga
     1861 cattgacgct cggtaggtaa aaccgcactt tctttgcaac tatagttttt ctttgcctcg
     1921 actgctcttt gcgacgcctt cctcactccc tcgagcacgc acacatattt tccgcgcgaa
     1981 tttacaaaca gtggatcatg cgctgcgtcg atgtgccccg cgacgtctca aagtcattgc
     2041 gtcaattggc tggtgataac cgcaaacata ttcctgtggt cggccaggta gaaggcttgc
     2101 cgctgaaaag cacgctctct cctcgcggtg gcggctctta ccgcctgcat ctacacagca
     2161 atatctggcg aaaacttcga atcgacgagg gtgccaccgt ggaagttatg ctgctcctag
     2221 acggcgaacc gcgggatcca gttttgccgc atgagctcgc gggcggcctt gccgatgcgc
     2281 cgcgcgctct ggccaccttt aattcattaa caccggcatt tcgcaggcaa atcgtccgct
     2341 atgtggagca ggtaaaacac gccagcactc gcgaaaaacg catccgctta atcgtcaaac
     2401 gcatgctcga acgcgccgct aaacagaaga agaagaaaac agcctgattt attttggctc
     2461 agcaatagaa gccatgcgtt tctgtgaagc tagccaagga actagctgcg gttccgcgaa
     2521 cgcttcattc catacttca
//
LOCUS       DATCBB010000076         1473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_2409, whole genome shotgun sequence.
ACCESSION   DATCBB010000076 DATCBB010000000
VERSION     DATCBB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..572)
                     /locus_tag="VII37_00910"
     CDS             complement(183..572)
                     /locus_tag="VII37_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068304.1"
                     /translation="MPADTQKIAMQLDADRRLSAAPGGVGRYFADAAGLANSAVSGLQ
                     SAIVAACNREFELIGDSRQRLDVTLTRTPDRIEVVMRRRATSDGAKKDLAEQGAIPGV
                     DQVQHETRAEMAITRLTKYVGEEASAG"
     gene            980..>1473
                     /locus_tag="VII37_00915"
     CDS             980..>1473
                     /locus_tag="VII37_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068305.1"
                     /translation="MVNFGGWDMPVEYQSSGGLVAEHKAVRASVGVFDVSHMGDIRIA
                     TARAHGGALAAVQHITMNDAAKLAIGQAQYSALLYPSGTFVDDVIVHRLGDDDFLLVI
                     NAGTREKDINWVRENTASHDCKVEELSDAYTQLAIQGPRAIEMLQKLTDVNLHPIKNY
                     RFTHG"
BASE COUNT          325 a          439 c          380 g          329 t
ORIGIN      
        1 ggctcagcag gataatcctg aaatggccca gcttctcgat tcggtgcgcg tcactccggc
       61 gggcgaccga gtcgtcttgc gcatgaccct aagcgacgat caagttactt ctctaatccg
      121 caaaaacacc ttcgcgttca aaatgtaact ttcgctacgt tggtcgaagc cgtgcgaaag
      181 cactaaccgg cggaggcttc ttcgcctaca tacttcgtca agcgcgtaat cgccatttcc
      241 gctcgagttt cgtgctgcac ttggtctacc ccaggaatcg caccctgctc tgccaaatct
      301 ttcttcgcac catcgctcgt agcgcgcctt ctcattacta cctcaatgcg gtcgggcgtt
      361 ctcgtcagcg tcacgtccaa gcgttgtctc gaatccccga taagttcaaa ttcccggttg
      421 cacgctgcga cgatcgcgga ttgcaatcct gaaacagcgc tatttgcgag tcccgccgca
      481 tccgcgaagt agcgtccaac tccgccagga gcggcgctca agcgccggtc agcgtccagt
      541 tgcatggcaa tcttttgggt gtcagcgggc acggttatca gctaaagaca catcgcaggc
      601 ttcccggcaa tccgggcaat agtacagccc atccatgcag agtcgcacgc cgacctggcc
      661 ttggcaaatg gtgcagtact tttcgcacgg acaatgctct tcggttttat cacactggac
      721 acaaacgacc tgtgcgggca tggaagcctc cgaaaaaact ctacctgagg actgactgcc
      781 cggcaaggcg ccggcgcagg tcaatgcgcg aatcaaatag ctgtacttcc agacttcctt
      841 tgtgcttttt gacccactag aaggtgcatg ctagtatcaa tttccgctga attttgtgga
      901 gtctaccgtc gatccgtccg tcattaccga gcttcgtaaa acgccattaa atgccgttca
      961 ccgccgtttg ggcgcgaaaa tggtgaattt tggcggctgg gatatgcctg tcgaatatca
     1021 gtccagcggc gggctggtcg ccgaacacaa agccgtgcga gccagcgttg gcgtttttga
     1081 cgtgagccac atgggcgaca ttcgcatcgc caccgcgcgt gctcacggcg gggccttggc
     1141 cgccgtgcag cacatcacga tgaatgacgc tgcaaagttg gccataggcc aggcgcaata
     1201 ttccgccctg ctttacccgt cgggaacttt tgtggatgac gtcatcgttc accggctcgg
     1261 agatgacgat tttcttttgg tgatcaacgc cggaacccgc gaaaaggaca tcaactgggt
     1321 tcgtgaaaat accgcgtcac acgactgcaa ggtcgaagag ttgagcgacg cttacacgca
     1381 actggccatc cagggaccac gcgccatcga aatgctgcaa aaacttaccg acgtaaattt
     1441 gcatccgatc aaaaactacc ggttcaccca cgg
//
LOCUS       DATCBB010000077         1368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_326790, whole genome shotgun sequence.
ACCESSION   DATCBB010000077 DATCBB010000000
VERSION     DATCBB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326790"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VII37_00920"
     CDS             <1..527
                     /locus_tag="VII37_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068306.1"
                     /translation="RLAQRETEVVIQFRRTPFVLFRNTSVRNLETNRLVVHIQPDEGI
                     SLSFGAKVPGSIMKLGLVNMDFDYATYFGTEHSTGYERLLRDCMVGDATLFQRADMVE
                     AGWSVIQPILDVWHALPPRDFPNYAAGSWGPPEAKELLERDGREWHNAGDEGLGRRNR
                     TNDRVNAADRVGIA"
     gene            527..1300
                     /gene="pgl"
                     /locus_tag="VII37_00925"
     CDS             527..1300
                     /gene="pgl"
                     /locus_tag="VII37_00925"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY5068307.1"
                     /translation="MPAAAKQREVYILKDNAAIARRAAEEFVKLAQSAVQQHGSFSVA
                     LSGGSTPKLLYSLLATDADLRAKVPWDKMQLFFGDERHVGPDDPQSNFKMAAESMFNK
                     APLKPEQIHRMKGEYPDAEKAAQEYEQDLRTHFHLKDKELPRFDLVLAGMGDEGHTLS
                     LFPGTKALYPEGRAVVRNWVGKLDTDRITITAPTANNAAMILFMVAGKDKAPALKSVL
                     EGPYEPEQLPAQLLQPTNGRLLWLVDEAAGSMLSNAIRE"
BASE COUNT          333 a          357 c          390 g          288 t
ORIGIN      
        1 aacggctggc acagcgtgaa actgaagtcg tgattcaatt ccgacgcacg ccgtttgttt
       61 tatttcgcaa caccagcgtg cgaaatcttg aaacgaatcg tctggtcgtt cacattcaac
      121 ctgacgaagg catttctctg agcttcggcg cgaaagttcc ggggtccata atgaagctgg
      181 gcctggtaaa tatggatttt gattacgcga cttatttcgg aacggagcac agcactggtt
      241 acgagcgtct gttgcgcgat tgcatggtgg gcgatgccac gcttttccag cgcgccgaca
      301 tggtggaagc tggctggagt gtcattcagc ccatactgga cgtctggcat gctttgcccc
      361 cacgtgattt cccgaactac gccgcggggt catgggggcc tcccgaagct aaggaattgc
      421 tggaacggga tggccgcgaa tggcacaacg ccggcgacga aggtcttggg cgccgcaacc
      481 gtacgaacga ccgagttaat gcagcggatc gtgtgggaat cgcgtgatgc ctgcggccgc
      541 aaaacagcgc gaagtttata ttctgaagga taacgctgca attgcgaggc gcgcggctga
      601 agagtttgta aagctagcgc aatccgcggt tcaacagcat ggatctttct cagtagcgtt
      661 gtctggcggc tctactccga aattgcttta ctccttgttg gccacggacg cggatctgcg
      721 cgccaaagtt ccctgggata aaatgcagtt gttcttcggc gacgaacgcc acgtgggacc
      781 ggatgacccg cagagcaatt tcaaaatggc tgcggaaagc atgttcaaca aagcgccgct
      841 aaaaccagag cagattcacc gcatgaaggg cgagtatccg gacgccgaaa aagccgccca
      901 ggaatacgag caagacttgc gcactcattt ccatctaaaa gataaggaac tgccgcgctt
      961 tgacctggtt ttagcgggca tgggcgacga agggcacacg ttgtcgcttt ttccgggaac
     1021 gaaagctctg taccctgagg gtcgcgccgt agttcgcaac tgggtcggta aacttgatac
     1081 tgaccgcatc acgatcaccg cgccaacggc caacaatgct gccatgattc tttttatggt
     1141 cgccggaaaa gacaaagcgc ccgctctaaa atcggtgctc gaaggtcctt acgagccgga
     1201 acagttgccg gcacaattgc tgcagcccac caatggaaga ttactgtggc tggtcgacga
     1261 agccgcaggc agtatgcttt ctaacgccat tcgagaataa acaggagaac tatatggcca
     1321 ccagtacaag caccggaaag atcgaaacct atttgggagc caaggcgg
//
LOCUS       DATCBB010000078         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_359283, whole genome shotgun sequence.
ACCESSION   DATCBB010000078 DATCBB010000000
VERSION     DATCBB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359283"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VII37_00930"
     CDS             complement(<1..1258)
                     /locus_tag="VII37_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068308.1"
                     /translation="MGTVLRNLRYAARALRANPGFAVIAVLTLALGIGANAAIFSVVY
                     TALLRPLPYYEPQRLMSLGESREQLANLQFSQVSYPDYLDWRKQAKSFSSIAVYSGDG
                     FTLRIADEPKNVFATQVSANFFSTLGVKPLLGRDFVDGDVQADGLHVAMLSYRFWQSD
                     FGGDKNVLGRVVQVDNMPATIVGVLPRNFEFFNSTPMWVPIHQSGEFMARRNLRWLNV
                     VGRLVPGATAEQARAEMDGVTGRLVAAYPKENGSTIFVMKTLRENFVGQVRTLLLILL
                     GAVGFVLLIACANVANLLMTRSIGRRKEFAVRVALGANRGNILSQLLTESLLLAAMGA
                     ALGLAGAQWGVSLLLAAIPEPILQSMPYLRDAGINVPVLGFLSGVTVLTAIIFGLAPG
                     LTIARGSVNAALKDETRGGTSTAQARL"
     gene            complement(1752..2195)
                     /locus_tag="VII37_00935"
     CDS             complement(1752..2195)
                     /locus_tag="VII37_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5068309.1"
                     /translation="MRGLKIPEELNTNPCRRRGARRFGFTLIELMIVITIIFILLGLA
                     AGRYDRSVVRAREATLKQDLQVMRQAIDNYTLDKQSAPQSIEALQEAGYLRIVPTDPM
                     TQAKDWQPQFDEVVLSPDQTGTGMVDVHSNSDKSSPFDQTPYKEW"
     gene            complement(2205..>2585)
                     /locus_tag="VII37_00940"
     CDS             complement(2205..>2585)
                     /locus_tag="VII37_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068310.1"
                     /translation="ALPIAKFTVIRTKESDLHRDLRDMRDAIDRYKDAADRNQIRVEL
                     GSEGYPPDLDTLVKGVQLGAASDKKIRFLRKIPVDPMTGQAEWGLRAVQDDPDSTSWG
                     GKNVFDVYSKSQGVAQDGTKYSDW"
BASE COUNT          609 a          832 c          601 g          543 t
ORIGIN      
        1 gcaggcgcgc ttgagccgtg ctggttcctc ctcgcgtttc atccttcagg gctgcattca
       61 ccgagccccg cgctatcgtc aatcccggcg caagtccaaa aataatggcc gtcaagaccg
      121 ttactccgct caagaatcca aggacaggca cgttaattcc cgcatcccgc aaatacggca
      181 tcgattgcaa tatcggttcg gggattgccg ccagcaacaa cgacacgccc cactgcgccc
      241 cggccaaacc cagtgccgcg cccatcgctg ccagcagcag gctttccgtc aacaattgcg
      301 ataaaatatt tccgcggttc gctcccaagg ccacccgaac cgcaaactcc ttgcgccgcc
      361 cgatcgatct cgtcatcaaa agattcgcta cgtttgcgca cgcaatcagc agcacaaatc
      421 ccaccgcccc cagcaagatc aacagcagcg tccgtacctg accaacaaaa ttctcccgca
      481 gcgttttcat tacaaaaatg gtcgagccgt tttcttttgg atacgccgcc accaatcgcc
      541 ccgtcactcc gtccatctcc gcgcgggcct gctccgccgt tgcccccggg accagccggc
      601 ccacgacgtt cagccatcgt aagtttcgcc gtgccataaa ttcgccgctc tgatgaatcg
      661 gcacccacat cggcgtagag ttgaaaaact caaaattccg cggcagcact cccacgatcg
      721 tcgcgggcat gttgtccacc tgcaccacgc ggcccagtac gtttttatcc ccgccaaaat
      781 cgctttgcca gaatcgataa ctcagcatgg ccacgtgcaa tccgtcggcc tgcacgtccc
      841 cgtcgacaaa atcgcgtccc agcaaaggct ttacgcctag ggtggagaaa aagttggccg
      901 acacctgggt cgcgaacacg ttttttggtt catccgcaat ccgtaacgta aacccatccc
      961 cgctgtacac tgcgatcgac gaaaaactct tcgcctgctt ccgccagtcg aggtaatcgg
     1021 gatacgacac ttgcgaaaat tggagattcg ccagttgctc ccgcgactcc cccaacgaca
     1081 tcaagcgctg cggctcatag tagggaagcg gtcttagcag cgccgtatac accaccgaaa
     1141 agatcgccgc attcgcacca atccccaacg ccagggtcag caccgcgatc accgcaaagc
     1201 ccggattcgc gcgtagcgca cgcgccgcat aacgcaagtt ccgcaaaacg gttcccatct
     1261 gagcctccgg ctatacctcg cacccgagct tgcacgccga tcaccgcaca aaaactagca
     1321 gcgctattta ccggtgcttc aatccacacc gtttagcaaa ctcactgcac caccagtccc
     1381 gcccgaaccg cccgataatg ggaaactgca caacaaaagc gcacacttcc tgaggatcgg
     1441 tcccacggta ggttacgtcg aatcgcttat tgtttcccgt caaacaggca agtctcaaac
     1501 tgacgaggcg gcgaattttc cggatttgta tcgacgccgt ctgagtaggg cggaatcggc
     1561 agtcatggga gaatgtccgg caaaaaaaca gcggtctggt aggcttagtg actagagtac
     1621 ggatctcttg aattagctca ggcctccagc tcgacccagc cgaccgcgca aataatccct
     1681 gattttaatt gagtgatttt tggtgccgcg cgccgataaa cgagtgtctt tcgaaaaaca
     1741 aacccgatca gttaccactc tttatagggg gtctgatcaa acggcgacga tttatctgaa
     1801 ttggaatgca catcaaccat tccggttccg gtctggtccg gactcaacac cacttcatcg
     1861 aactgcggtt gccaatcttt tgcttgggtc atcggatcgg tcggcacgat gcgcaggtac
     1921 ccagcttcct gcaaagcctc gatggactgc ggagcggatt gcttatcgag agtgtaatta
     1981 tcgatggcct ggcgcatcac ctgcaagtcc tgcttcaagg tagcctcgcg agcgcgaacc
     2041 actgagcggt catagcgtcc cgctgcaagc cccagcaaaa taaaaataat tgtaattaca
     2101 atcatcaact cgatcagcgt aaagccaaag cgacgcgcac cccgccgccg acaaggattc
     2161 gtgttcagct cttccggtat tttcaatcca cgcatactcg caaatcacca atctgaatat
     2221 tttgtgccgt cctgcgcaac accttgcgac ttcgaataca catcgaatac attttttccg
     2281 ccccaactcg ttgagtcggg atcatcctgc accgcacgca aaccccattc ggcctgcccg
     2341 gtcatgggat cgactggaat tttgcgcagg aagcggatct tcttatcgct cgcagcgccc
     2401 aattgcacgc ccttcaccag tgtgtccaaa tccggtggat agccttcact gccgagttct
     2461 actctgatct ggttgcgatc ggcggcatcc ttgtagcgat caatggcgtc ccgcatatcg
     2521 cgcaaatctc ggtgaagatc actttcctta gttcgaataa ccgtgaactt ggcgatcggc
     2581 agggc
//
LOCUS       DATCBB010000079         1385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_100071, whole genome shotgun sequence.
ACCESSION   DATCBB010000079 DATCBB010000000
VERSION     DATCBB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100071"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..959
                     /locus_tag="VII37_00945"
     CDS             120..959
                     /locus_tag="VII37_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY5068311.1"
                     /translation="MLPIEKSRLSMGQAGLICGWGIFTTIRIARGEAFAYERHWRRLD
                     KHAAIIKLPMPYAPAKVRMHIHEVIRANKVTEGCARVYLVYNSVGFWQSDEPRPQVDL
                     LICSAGLPQYGESARLGLRAHGRHAAAALSGIKSISWLNNVWAVAEAAKEGFDEVVLL
                     NERGEVSECTSANIFAVKNGKVLTPPLNSGCLEGVTRGILMEIAPEAGVSVAEQSLRP
                     EDLYSADEVFISSTNRNVIGVGEIAGHKIAASPGRVTQRLNELFDAYIDDYVTRRMST
                     SAR"
     gene            1111..>1385
                     /locus_tag="VII37_00950"
     CDS             1111..>1385
                     /locus_tag="VII37_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068312.1"
                     /translation="MMESLGSDLRFGARTLWKSRAFSAIAIITLALGVGASTAIFSVI
                     DNILLEPFPYPHAQRLMSLEIHNTDESNPGGRGAFSGAEFLDYSTQN"
BASE COUNT          312 a          392 c          358 g          323 t
ORIGIN      
        1 ctcctctgtt accgagaagc ggcaactgga aatttgcgcc attgaatcgg ttacaatgag
       61 catgcggccc tcaccgcgag gatcccttga ttcatcgcca catcttccac aatgaaagct
      121 tgttacccat tgagaagtcc agactttcca tgggccaggc cgggctcatc tgcggctggg
      181 gcattttcac aactatacgt atcgcgcgtg gcgaagcttt cgcatacgaa cgccactggc
      241 ggcggctgga caagcacgcc gcaattatta aactaccgat gccctacgca cccgccaaag
      301 tgcgcatgca tattcatgaa gtaatacgcg ccaacaaagt taccgaaggc tgcgccagag
      361 tctatcttgt ttataacagc gtaggattct ggcaaagcga cgaaccgcgc cctcaagtcg
      421 atctcctgat ttgttcggcc gggcttcccc agtatggaga gagtgcccgt ctggggctgc
      481 gtgcccacgg tcggcacgcc gctgcggcgc tttcgggaat caagagtatt tcctggctga
      541 ataatgtctg ggcagttgcg gaagctgcaa aggaaggctt cgatgaggtt gtgctactga
      601 acgaacgcgg cgaagtgtcg gaatgtacct ccgctaatat tttcgcggtg aaaaacggca
      661 aagtgcttac gccgccacta aactccggct gcctggaagg cgtcacgcga ggaattttga
      721 tggaaatcgc ccccgaagcc ggtgtatccg ttgcggagca atctttacgc cctgaagatt
      781 tgtattccgc ggacgaagtt ttcatttctt cgacgaatcg caatgtcatt ggagtcggcg
      841 agattgccgg tcacaaaatc gccgcttccc cgggccgtgt cactcagcgg cttaatgagc
      901 tcttcgacgc gtatatcgac gactacgtaa cccgtcgcat gtccaccagc gcgcgctaac
      961 ttcttccgca aaaacttgcc tttcgggaac tttccatcat ttctccacgt taatgagaga
     1021 gatgatattt gttcgtacca accttttgat gggttattcg tctcaaacat agtcaacacc
     1081 gctggacggc gtccggaagg aaaaattgtc atgatggaat ccctgggttc cgatctccgc
     1141 tttggagccc gcacgctgtg gaaaagccgt gccttctccg ccatcgccat catcacgctg
     1201 gcactgggtg tgggcgcttc gacggcgatt ttcagcgtga tcgacaacat tctgctggaa
     1261 ccatttccct atccacacgc acaacgcttg atgtcccttg agattcataa caccgacgaa
     1321 agcaatccgg gcgggcgcgg cgccttttcg ggcgccgaat tcctggatta ttccacgcaa
     1381 aacca
//
LOCUS       DATCBB010000080         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_132396, whole genome shotgun sequence.
ACCESSION   DATCBB010000080 DATCBB010000000
VERSION     DATCBB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132396"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2192)
                     /locus_tag="VII37_00955"
     CDS             complement(<1..2192)
                     /locus_tag="VII37_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY5068313.1"
                     /translation="MGVEDNFFELGGDSILSIQIISAARREGLKLTPKLLFANQTIAS
                     LAGVAGVTESDNTAAVEVAGDVPLTPIAKWFFEQNLDDLHHYNQAFLFTVTENLDRVV
                     LEQALAELGRHHDALRLRAALDNGTWKIFYSGSSEAAPLEWIDISKRSESEQAATIES
                     ISAKQQASLNIQRGPIWRVVYFNLGNRRPGRLLFAVHHLAVDGISWRPLLEDLETAYQ
                     QIKAKQKVVLPAKTASYKTWAEHLQALAKSESLKQELAYWTTVTDPSAVADAMKPLAM
                     DASASAKNTEGNANKMTVALDADLTRALLQTVPTVYNTQINDVLLTALARGWVKWTGS
                     RTLYTNLEGHGRENLFEDVDVSRTVGWFTSIFPVRLELNDSRENWKPGEALKAVKEQL
                     RRIPKRGVGYGILRYLSGDGALTSRPEPAMVFNYLGQFDRAVESSKLLRFANESSGPW
                     HSPQQKRRHVLEINSLVMNDRMEFEFTYDPGLHDAKRIQQFADEFLNALKEVIAHCEL
                     PDAGGRTPSDFPLARLDQPSLDRLLAEQPGIEDIYPLSPMQTLFFSANQGSAQAAFDQ
                     WHSTLRGDLGVDAFKRAWNETIRRHTILRSTVVSAGLREPVQAVHREVRPPWIMEDWR
                     GTTAEQQRERWNALLKQDRGEALDLTQAPVMRFKLLRFNDTTWRFLWSVPSMLMDGWS
                     WPLVFRDASRLYEAYARDVAPQLEAPRPYRDYLEWLGKQPAAESAEFW"
BASE COUNT          507 a          691 c          571 g          540 t
ORIGIN      
        1 tgccagaatt ccgctgattc agctgccggc tgcttgccta gccactccag ataatcgcgg
       61 tacggccggg gcgcctccag ttgcggagca acatcccggg cataggcttc ataaagtcta
      121 ctggcgtcgc gaaaaacgag cggccatgac cagccatcca tgagcatgct tggcacgctc
      181 cacaaaaatc gccaggtggt gtcgttaaac cgtagcagct tgaatcgcat caccggcgcc
      241 tgcgtcaaat ccagcgcttc gccgcgatcc tgcttcagca gcgcgttcca cctctcccgt
      301 tgttgttcgg ctgtagtacc tcgccaatcc tccattatcc agggcgggcg tacctcgcga
      361 tgcacagctt gcaccggttc gcgcaacccg gcactcacga ccgttgagcg caaaatcgtg
      421 tgccgccgga tcgtctcatt ccaagcgcgt ttgaacgcgt ccacacctaa atccccacgc
      481 agagtggaat gccactgatc gaaagcagct tgcgcggagc cttggtttgc agaaaagaac
      541 agcgtctgca tcggcgatag cgggtaaata tcttcgattc ccggctgctc cgctaacaaa
      601 cgatcgagcg aaggttgatc gagccgcgcc agcggaaaat ctgaaggagt gcgtccacca
      661 gcatcaggca attcacaatg cgcgatcact tcttttagtg catttaaaaa ctcatctgca
      721 aactgctgga tgcgcttcgc atcatgaagt cctgggtcgt acgtaaattc gaattccatc
      781 cggtcattca tgaccaggct gtttatttcc agaacgtgcc gccgtttctg ctgggggctg
      841 tgccatggtc cgctggattc gttagcaaat cgcagcagtt tcgagctctc aacggcgcga
      901 tcgaattgcc ccaggtagtt gaacaccatc gctggctccg gtcgcgatgt gagcgcgccg
      961 tcaccgctca aataacgaag aattccgtaa cctacgccgc gcttcgggat acggcgtaat
     1021 tgttctttga cagccttcaa ggcctcgccg ggcttccaat tctctctgga atcattcaac
     1081 tccaaacgta ccgggaagat cgatgtgaac catcccacgg ttctgctgac gtccacatcc
     1141 tcaaataaat tttcccggcc atgcccttcg agatttgtgt acaaagtacg gctgccggtc
     1201 catttcaccc acccgcgagc cagcgccgtc agcaacacat cgttaatctg cgtgttgtag
     1261 accgtcggaa ccgtttgcag caaagctcgc gttaagtctg cgtccagcgc cactgtcatt
     1321 ttgttcgcat taccttcggt gtttttcgcg gatgccgaag catccatcgc caaaggcttc
     1381 attgcatcag cgaccgcgct ggggtcggtg accgtggtcc aataggccaa ttcctgtttc
     1441 aacgactccg atttcgccag tgcctgcaag tgttcagccc acgtcttgta cgacgcagtc
     1501 ttggcgggca aaacgacttt ttgtttcgcc ttgatctgtt ggtacgccgt ctccaaatcc
     1561 tctaacagcg gccgccacga aatcccgtct accgccaagt ggtgcactgc aaagagcagt
     1621 cgtcccggcc gtctattgcc caaattgaaa tacaccacac gccaaatcgg cccacgttga
     1681 atatttaggc tggcttgctg tttcgccgag atcgattcga tggtcgcggc ttgttccgac
     1741 tctgagcgct tagagatatc tatccattcg agcggcgccg cctccgaaga gccggagtaa
     1801 aatattttcc atgtgccgtt atccagcgct gctcgcaatc gcagcgcatc atgatgccgg
     1861 cccaattccg caagcgcctg ttccagaacc acgcgatcca gattttcggt caccgtaaaa
     1921 agaaaagctt ggttgtaatg gtgcaaatcg tccaggtttt gttcgaagaa ccacttcgcg
     1981 ataggagtga gaggtacgtc acctgcgact tctacagcag cagtgttgtc actttccgtc
     2041 acacccgcaa ccccggcaag agacgcgatg gtttgattcg caaacagtaa ttttggcgtg
     2101 agcttcaacc cttcgcgtcg agcagcagaa ataatctgaa tactcagaat ggaatcgccg
     2161 cccagctcaa aaaaattatc ttcgactccc acattttcta cgcggagcgc ttttgaccac
     2221 actgccgata atgttttttc cgctgccgtt cgcggcgcga tatatcgtct ggtcaaatcg
     2281 ggccgctggc tttccggaac cggcaaagc
//
LOCUS       DATCBB010000081         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_326940, whole genome shotgun sequence.
ACCESSION   DATCBB010000081 DATCBB010000000
VERSION     DATCBB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326940"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /gene="pnp"
                     /locus_tag="VII37_00960"
     CDS             complement(<1..1717)
                     /gene="pnp"
                     /locus_tag="VII37_00960"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY5068314.1"
                     /translation="MSEQSHIPAPHQVTVEVGGRPLILETGKVAKQANGAILARYGDT
                     IVLTTACMAPTANDRDFLPLTVDYRENTYSAGKIPGGFFKREGRPSEKEILTSRLIDR
                     PMRPLFPDSWRNETQIVSMVLSADSDNDPDVIAVTGASAACYCSDLPFEKPVACVRVG
                     LLDGQLVANPTVADQKKSLMNIVIAGTEDAIVMVESGALEVSEEAVVDALEFGHAQIK
                     KIVGAIKELHAQLKIKKVTVPPLPFDESLYKEVEKKYGARLHDAVNTEKHPKKESYLL
                     IDAIKDELKVSIPEEEEEKRVLAKRAFDRLRENIFRDDVLNKRRRPDGRGFDQIRHIS
                     CEVGLLPRVHGSALFTRGETQALATLTLGTKEDMQRQDLLFAQDQFKRFMLHYNFPPF
                     SVGEVKFLRGPGRREIGHGALAERALLSLLPPEADFPYAMRLVSDILESNGSSSMATV
                     CGGSLALMDAGVPLKAPCAGIAMGLVVGDDMKYSILTDIAGAEDHYGDMDFKVAGTRD
                     GITALQMDIKVLGISIAMMREALAQAKKARLQILDTMEKTISEGRTQISAFAPRLFKL
                     KIPQDK"
     gene            complement(2066..2308)
                     /gene="rpsO"
                     /locus_tag="VII37_00965"
     CDS             complement(2066..2308)
                     /gene="rpsO"
                     /locus_tag="VII37_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY5068315.1"
                     /translation="MLIEQYRTHIKDTGSPEVQIALLSERINYLTTHLKSHVKDHASR
                     RGLIMMVNKRRRLLDYLNRRDPDRYREIVERLSLRK"
BASE COUNT          486 a          669 c          616 g          538 t
ORIGIN      
        1 tcttgtcctg cggaatcttc agcttgaaca gacgcggcgc aaaagccgaa atctgcgtgc
       61 ggccttcgct gatcgtcttt tccatggtgt cgagaatctg caagcgggct ttcttggctt
      121 gggccagcgc ttcacgcatc atggcgatgc tgatgcccag aaccttgatg tccatctgca
      181 gcgcggtaat tccgtcccgc gttccagcta ccttgaagtc catatcgccg taatgatctt
      241 ccgcaccggc gatatccgtc aggatcgaat acttcatatc atcgccgacc accaagccca
      301 tggcgattcc cgcgcatggg gccttcagcg gtacacccgc atccatcaaa gctagcgagc
      361 ctccgcaaac agtggccata gatgacgaac cattcgattc cagaatgtcc gataccaggc
      421 gcatggcgta ggggaaatcc gcctcaggtg gcagcaagct taaaagcgcg cgttccgcta
      481 acgcgccgtg accgatttca cggcgtcccg ggccacgcag aaacttaacc tcgcctacgc
      541 tgaatggcgg aaaattgtag tgcagcataa aacgcttgaa ctgatcctgc gcgaagagca
      601 ggtcctggcg ctgcatatct tccttggtgc cgagggtcag agtggccagc gcttgcgtct
      661 caccgcgagt aaacaacgcg gagccgtgca cgcgtggcag caaacccact tcgcacgaaa
      721 tgtgacgaat ctgatcgaag ccgcgcccat ccggacgccg gcgcttgttc agcacgtcat
      781 cgcggaagat attctcgcgc agacggtcaa aggcccgctt ggccaaaact cgcttctctt
      841 cctcttcttc cgggatcgaa accttgagct cgtccttgat ggcgtcgatc aacaggtagc
      901 tttctttctt ggggtgcttc tcggtgttca ccgcgtcatg caatcgcgcg ccatacttct
      961 tttcgacttc cttatagagc gattcatcga acggcagcgg cggcacggtg accttcttga
     1021 tcttcagttg cgcgtgcagt tccttgatgg ctcccacgat ctttttgatt tgcgcgtgtc
     1081 caaattcgag cgcatccacg accgcttctt cagagacttc cagggcgccg gactccacca
     1141 tgacaatggc gtcttccgtt ccggcgatca caatgttcat cagacttttt ttctgatccg
     1201 ctactgtggg gttggcgacc agttggccgt ccagcaatcc aacgcgaacg caggcgacgg
     1261 gcttctcaaa cggcaggtcc gagcaatagc aggctgcgga agctccggta accgcaatca
     1321 catcgggatc gttgtcgctg tcggcagaca acaccatgct gacaatttgt gtttcgttgc
     1381 gccaggaatc ggggaacaaa gggcgcatgg gccgatcgat caaacgtgaa gtcaaaattt
     1441 ctttttccga ggggcgccct tcgcgcttaa aaaatccacc aggaatcttt cccgctgaat
     1501 aggtgttttc ccggtaatcc acggtcaatg gcaaaaagtc gcgatcattc gcagtgggtg
     1561 ccatacaagc ggtggtcaat acaattgtgt cgccgtagcg tgccagaatc gcgccattgg
     1621 cttgcttggc tactttgccg gtttccagga tcagcggacg cccgccgact tcaacggtta
     1681 cctggtgtgg tgctggtatg tgtgactgtt cagacattta ttctttcctt tagcttcgcg
     1741 ttcgcgcaaa gcattgcaaa aagcgtcagc cgttgcacgg ctgcgcgtaa aactttctct
     1801 gctggcagtt ggccggccat cgaggcgacc ggttaaagaa gacaagaggt ggttgaacgc
     1861 tttgtgggac tgcaaaactc caacggatca caccgcgaaa aacgcctaga ttgcagcctt
     1921 atcgtcggcg caagtcgaca acgtacacga aacgcgtgcc ttcgagccgg agctcacact
     1981 accggcagac agactcccag tggaatttgt cacttcgcga ttgcgttgcg gattccgccc
     2041 caatttcccc gtgcttccag ggcggttact tgcgcaggct aagacgttca acaatctcgc
     2101 ggtaccgatc cgggtcgcgg cggttgagat agtccagcag ccgccggcgt ttgttcacca
     2161 tcataatcaa accgcgacgt gatgcgtggt ctttgacgtg cgacttgaga tgcgtcgtca
     2221 gatagttgat ccgctcgctc agcagcgcaa tttgcacttc cggtgatcct gtgtccttaa
     2281 tgtgagtgcg atattgctcg atcaacact
//
LOCUS       DATCBB010000082         1058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_392050, whole genome shotgun sequence.
ACCESSION   DATCBB010000082 DATCBB010000000
VERSION     DATCBB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392050"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VII37_00970"
     CDS             <1..237
                     /locus_tag="VII37_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068316.1"
                     /translation="FASAQVLTRDLDTETKVVVNTFWKNLASIEAFAGSDRDAALVAP
                     DAAALLTSYDKCVRHYLVAVSDSSESVSLFDDRD"
     gene            complement(234..>1058)
                     /locus_tag="VII37_00975"
     CDS             complement(234..>1058)
                     /locus_tag="VII37_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5068317.1"
                     /translation="LADPEIEAIYNPLPNHLHVPWSIKAMEAGKHVLCEKPIALNAAE
                     ARTLLPVRDRAGVKIGEAFMVRTHPQWRRTKELIDKGRIGELRAVVGVFSYFNRDAKN
                     VRSKPEWGGGGLMDIGCYPITTSRFMFGAEPLRVSGVLERDPEFGTDRLASAILEFSG
                     GQVVFTCSTQMAPYQRMQFLGTKGRIEIEIPFNAPPNKATRIFLDDGSDVGGAGIKIE
                     TIPACDQYTIQGDVFSKAILENGQVPVPLEDAIANMAVIDAIFRSAESGRWEKPRG"
BASE COUNT          238 a          342 c          240 g          238 t
ORIGIN      
        1 ttcgcgtccg cgcaggtgct gacgcgcgat ctggatactg aaacgaaagt agtagtgaac
       61 acgttctgga aaaatttggc ctccattgag gccttcgcag gatcagatcg cgatgctgcg
      121 ctggtggccc ctgacgcggc cgccttgctg accagctatg ataagtgcgt aaggcattac
      181 ctggtagccg tctccgacag ttccgaatcg gtctcgctgt ttgacgaccg tgactagccc
      241 ctcggcttct cccacctccc ggattccgcc gaacgaaaaa tcgcatcaat caccgccatg
      301 ttggcgatag catcttccaa cggtactggc acctgcccat tttcaagaat tgccttcgag
      361 aaaacatctc cctgtatcgt gtactgatca caagcaggaa tcgtctcaat cttgattccc
      421 gcgcctccca catcactgcc atcatccaaa aaaatccgcg tggccttgtt gggcggcgca
      481 ttgaacggaa tctcgatctc aattcttccc ttcgtgccaa gaaactgcat acgctggtaa
      541 ggtgccatct gcgtgctgca tgtgaacacc acctgcccgc cgctgaattc cagaatcgcg
      601 gaagccaacc tgtccgtgcc aaactctgga tcgcgttcca aaacgccgct cacccgcaga
      661 ggctccgcac caaacataaa acgcgacgtg gtaatcggat aacatccgat gtccatcagc
      721 cccccgccgc cccactccgg cttgctccgt acatttttcg cgtcacgatt aaagtaactg
      781 aacactccca caaccgcgcg tagttcccca atgcgtccct tatcaatcaa ttccttcgtg
      841 cgccgccatt gcgggtgtgt ccgcaccata aatgcttcgc caatttttac gcccgcgcga
      901 tcccgcacag gcaacaaagt cctcgcctcc gccgcgttca acgcaatcgg cttttcgcaa
      961 agaacgtgct ttcccgcttc catggctttg attgaccacg gcacgtgcaa atgattgggc
     1021 aacggattat aaatagcctc aatctcggga tcagcgag
//
LOCUS       DATCBB010000083         1248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_294916, whole genome shotgun sequence.
ACCESSION   DATCBB010000083 DATCBB010000000
VERSION     DATCBB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294916"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VII37_00980"
     CDS             <1..665
                     /locus_tag="VII37_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5068318.1"
                     /translation="SGTERVLDIATGPGYIAEAFAKNSREVVGMDLTEAMLGIARART
                     AEHGVKNISFRTGDAQNLPFDAGEFDVVVCRLALHHVQQPAKVVSGMARVCRRDGIVL
                     VEDIYASEHSARAAYQDRWEILRDSSHVRALPISELLHLFRDAGLETETVSTADDLCP
                     EIERWMATTKVPAERATEIRALLEDDLQHDLSGTRPFRDAAGQMFFHARTVILTGRKT
                     GA"
     gene            961..>1248
                     /locus_tag="VII37_00985"
     CDS             961..>1248
                     /locus_tag="VII37_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068319.1"
                     /translation="MKELVKATVATVLAVLCAMPLPAAPGALKSTRTRRAPARYHGHG
                     VPTFADSSNGDITAFDDPVVRQAAVEGLGRYNGAVVAVDPNTGRVLTVVNQK"
BASE COUNT          286 a          300 c          389 g          273 t
ORIGIN      
        1 taagcgggac agaacgcgtg ctggatattg ctactggtcc gggatacatc gcggaagctt
       61 ttgcaaaaaa ttcgcgggaa gttgtgggaa tggatctgac ggaagcgatg ctgggcattg
      121 cccgcgcgcg caccgcagaa cacggcgtca agaatatttc ttttcgtact ggcgatgcgc
      181 aaaatctgcc cttcgatgca ggcgagtttg acgttgtggt gtgccggctt gctttgcatc
      241 atgtgcagca gcccgccaaa gtggttagtg gaatggctcg ggtatgccgt cgcgacggca
      301 tcgtgctagt ggaagacatt tatgccagcg aacattccgc gcgggccgct tatcaagacc
      361 gctgggaaat actgcgtgat tcctcgcatg tgcgggcgtt gcccattagc gaactgttgc
      421 atctttttcg cgacgcggga ttggaaacgg agacggttag cactgccgat gatctttgcc
      481 cggaaataga gcgatggatg gcgactacta aagtgcccgc ggagcgagcc acggaaatac
      541 gcgcgttgct ggaagacgat ttacagcatg atcttagcgg aacgcggcca tttcgcgacg
      601 ctgcagggca gatgtttttt cacgcccgca cggtgattct gacaggccgc aagacgggcg
      661 cctgaccttg tggtcagcta aaggccagct gatacaaagc aaagcacata cgttcgtact
      721 aagcctgata ggctattgcg gagcggattg cgtcaccata atatgtctca agtaagcggg
      781 agccgagttc gggcccgact tcagctgggt ggcatttcag taacaagccg catctgattg
      841 taaggaaaag gcgtaggctg gaacgacacc aagaatggta gttgaccggc gtgacagctt
      901 cgatgagaat caacgtcccg catagtttgt aacggaagat cgcactctga ggtggttgaa
      961 gtgaaggaac ttgtaaaagc gacagttgca actgtgctcg cagtgttatg cgcgatgccg
     1021 ctacctgcag cgccgggagc tttgaaatca acgcgcacac gcagagcgcc agcgcgttat
     1081 catgggcacg gagtacccac ctttgcggat tcttccaacg gggacattac ggcttttgat
     1141 gatccagttg ttcgtcaggc tgcggtcgaa ggattggggc gctataacgg cgcggtcgtg
     1201 gcggtggatc cgaataccgg acgagtcttg acggttgtga accagaag
//
LOCUS       DATCBB010000084         1578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_132478, whole genome shotgun sequence.
ACCESSION   DATCBB010000084 DATCBB010000000
VERSION     DATCBB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132478"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VII37_00990"
     CDS             complement(<1..204)
                     /locus_tag="VII37_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5068320.1"
                     /translation="MASIQKGTQSSLSEINMVPFVDVVLVLLIIFMITAPILQSGIEL
                     NVPKTHTVKESPEQRIVVSVDKQQ"
     gene            complement(253..918)
                     /locus_tag="VII37_00995"
     CDS             complement(253..918)
                     /locus_tag="VII37_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5068321.1"
                     /translation="MTDIGSILEQTGWVARGVLALLLIFSIVSWAMIFQKLGFFGRVK
                     REGDQFLRIFRATHGIANPQALAQAGSPYATVYAFGYRELQSQVSPNSANPSAGGRLK
                     SLSAVTVNMQLAAAEEVRRAEKGMSWLATTGSVSPFIGLFGTVWGIIDAFSGLGTAGA
                     ASLRAVAPGISEALFTTAAGLVTAIPAVIFYNQFLANIRNLAQRLDTFTLEVAAQIEK
                     TFT"
     gene            1091..>1578
                     /locus_tag="VII37_01000"
     CDS             1091..>1578
                     /locus_tag="VII37_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068322.1"
                     /translation="MSKAFDIGVESLSRSGGAENVGLVLNPSAHTGHIIHSSKFDVGG
                     RINVADGVVSGDATAPARSFGDTANGGTFFLETFGCQMNDHDSEKVAGLLLSRGYRQV
                     ETPEAARMILYNTCSIREKAAQKVFSRLGEYRERGADKIIGVLGCVAQQEGQDIFKRA
                     PWV"
BASE COUNT          354 a          348 c          536 g          340 t
ORIGIN      
        1 ttgctgcttg tcgaccgata cgacgatgcg ctgctccgga ctttctttaa cggtgtgtgt
       61 ttttggcaca ttcagttcga tgccggactg caggatgggg gcggtgatca tgaagatgat
      121 caggagcacc aacacgacgt ccacgaaggg gaccatgttg atctcggaga gcgaggattg
      181 ggtgcctttt tgaatggatg ccatgttcgt ccagattatg cgaggccagc gatgctgcgg
      241 cctggttgca atctacgtga aagttttttc aatttgcgcg gcgacttcta gtgtaaaggt
      301 atcgaggcgc tgcgccaggt tgcgaatatt cgccaagaat tgattataaa agattacggc
      361 tgggatggca gtgaccaaac cggcggcggt ggtaaaaagc gcttcggaaa tgccgggtgc
      421 gacggcgcgg aggctggcgg cgccggcggt gcctaggccg gagaaagcgt cgatgatgcc
      481 ccatacggtg ccgaataggc cgatgaacgg ggaaacggag ccggtggtgg cgagccagga
      541 catgcctttt tcggcgcggc gaacttcttc agcggcggct aattgcatat ttacagtgac
      601 ggccgagagg cttttcagtc tgccgccagc actaggattc gcggagttgg gcgaaacctg
      661 gctttggagt tcgcgataac cgaaggcgta tacggtggcg tagggagagc cggcttgcgc
      721 caaggcttgg ggattggcga ttccgtgcgt ggcgcggaag atgcggagga actggtcgcc
      781 ttcgcgtttg acgcggccga agaaacccag tttctggaag atcatagccc acgagacgat
      841 ggagaagatc aggagaagcg cgagaactcc acgggcgacc catccggttt gttcgagaat
      901 gctgccgatg tcggtcaaga agaccgtgct gaacagaaaa atggttaacc cctcagtgaa
      961 agcgaatttt tagtgactta agaaccgtag catacgcata gatgggacgc aacgaaacgt
     1021 aacactaatg gttacgagcg tttcgtgagc ggaaatgagc gaatcgagct atggtagact
     1081 gcgaattcag atgagtaagg ccttcgatat cggcgtggag tcgttaagca ggagcggcgg
     1141 cgccgagaat gtcgggctgg tactcaatcc ctcagcccac actggccaca ttattcattc
     1201 cagtaaattt gatgttggcg gcaggattaa tgttgctgac ggcgtggtgt ctggggatgc
     1261 gactgcgccg gctagaagtt ttggcgatac ggcgaatggc gggacgtttt ttctggagac
     1321 gtttgggtgc cagatgaacg accacgattc tgagaaagtg gcgggattgc tgttgtcgag
     1381 ggggtaccgg caagtggaga cgcctgaagc ggcgcgcatg attctttaca acacctgcag
     1441 cattcgcgag aaggcggcgc agaaggtatt ttcgcggttg ggagagtatc gcgagcgcgg
     1501 cgcggacaaa attattggcg tgctggggtg cgtggcgcag caggaagggc aggatatttt
     1561 taagcgcgcg ccgtgggt
//
LOCUS       DATCBB010000085         1060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_35270, whole genome shotgun sequence.
ACCESSION   DATCBB010000085 DATCBB010000000
VERSION     DATCBB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35270"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..758
                     /locus_tag="VII37_01005"
     CDS             213..758
                     /locus_tag="VII37_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068323.1"
                     /translation="MISVAGLAFASQSSNPAGQSTSEPAKPAQSPTASDPGSAESGKK
                     KPKKVWTNDEISKVGGEGSISVVGNADANAKGSPKGNPSKSTRASSAKENQPGSYRSR
                     LRQLHNQLETTEKQISDLRNFNGESTGTSGGVDMNRGYARTSVEDQIKILEEKKKQIQ
                     AQIGDIEDEARKDGIEPGDLR"
BASE COUNT          253 a          290 c          300 g          217 t
ORIGIN      
        1 gttttattcg aagtaccggc tggtcggaag tcccacggaa acgttggcgc tggtgcgttc
       61 tgaattgaag cagttgaagt aaggcacgcc ggacctgcgc gtgatagcaa attcggcata
      121 gcaaattaca ggttaagcct gccctacttc agatcgctta caatgaaaga aatgggggat
      181 ttatgaggcg cttccttttc ttatccgctc tcttgatttc cgtagcaggc cttgcattcg
      241 cttcgcaatc gtccaatcca gccgggcaat cgacatccga gcccgccaag ccggcgcagt
      301 cgccgacagc gtctgatcct ggttccgccg aaagcggcaa gaaaaagccc aagaaagtgt
      361 ggaccaacga cgaaatttca aaggtgggcg gcgaagggag catatcggtc gtcggtaacg
      421 cggatgccaa cgccaagggt tcgccgaagg gcaacccgtc gaaatctacg cgagcatctt
      481 ccgcaaaaga aaatcaaccc ggcagctatc gcagccggct tcggcaattg cacaaccagc
      541 tggagaccac ggaaaagcag atttccgacc tgcgcaattt caatggcgag agcacgggga
      601 cgtccggcgg cgtggatatg aatcgcggat atgcgagaac gtccgtggaa gatcagatca
      661 aaattctgga agagaaaaag aagcaaattc aggcgcagat cggcgacatt gaagacgagg
      721 cgcgcaagga tggaatcgag cccggcgacc tgcgctaatc gcaattaaac ggcgttccgc
      781 gggcgggcca ccatcgggca caagtgtcgt cacattcggt gacaatttct tcttcgattc
      841 cggcctttcc cccgatcgcg cactttccta taattagaag gcctcgcccg ggtgcggtcg
      901 cgtagttgcg acgcttaacc agctgttccc agcgaacagc ggagaaccct gcatgcgtcc
      961 ggttgtggtt gcccttatga gctttcgctg gaatgaattt gccactcgac caaggctggc
     1021 attttcttgc ctcctgctgt gcctgctgtc cggcatgccg
//
LOCUS       DATCBB010000086         1797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_67043, whole genome shotgun sequence.
ACCESSION   DATCBB010000086 DATCBB010000000
VERSION     DATCBB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67043"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VII37_01010"
     CDS             <1..657
                     /locus_tag="VII37_01010"
                     /inference="COORDINATES: protein motif:HMM:NF044723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068324.1"
                     /translation="DRNPATGGTIEIDCANLGSGAYSIPRSVEHLTFRTDARFVLAIE
                     TGGMFQRLNNHRFWETARCIIVEMGGVPTRATRRFIRLLSDKQNLPVYCFVDCDPYGF
                     ANIYRTLKVGSGNAAHINRFLCVPRARFLGVTPQDIIDFGLEDATHPLETTDIKRAQD
                     ALQNDPFIMAHPEWIAAIKQLLQMGVRAEQQALAKWGLNYVIDDYLPRKLANESSFLP
                     "
     gene            complement(1406..>1797)
                     /locus_tag="VII37_01015"
     CDS             complement(1406..>1797)
                     /locus_tag="VII37_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068325.1"
                     /translation="ATALRLLRPLAEQGDAKAQISLGAMYAGGQGVAQDYREAVKWYR
                     SAAEQGIASAQIVLGGMYADGRGVTQDYLRAHMWFNLAALKLSGDAGKWATESRDRIA
                     KSLTPAQVVQAQEMAKRCEQSYYKGCD"
BASE COUNT          401 a          528 c          462 g          406 t
ORIGIN      
        1 gacagaaatc ccgcgacggg agggacaatc gagatcgact gcgccaattt ggggagcggg
       61 gcctacagca tcccgcgctc ggtcgagcat ctgactttcc gcactgatgc acgattcgtg
      121 ctcgccattg aaaccggcgg catgtttcag cgcctcaata atcaccggtt ttgggaaaca
      181 gcgcgatgca tcatcgtcga gatgggcggg gtcccgaccc gcgccacgcg gcgcttcatc
      241 cgcctgcttt ccgacaagca gaacctgccg gtctactgct ttgtcgattg cgatccctat
      301 ggctttgcaa acatctaccg cacgctcaag gtgggttcgg gcaatgcggc gcatataaac
      361 cgcttcttat gcgtgccgcg ggcgcgcttc ttaggcgtca cgccacagga cattatcgat
      421 tttggtcttg aggacgcgac ccacccgctg gaaacgactg acatcaagcg cgctcaagat
      481 gcgctgcaaa acgatccatt cattatggcc catcccgaat ggatcgccgc catcaagcaa
      541 ttgcttcaaa tgggggttcg cgccgagcag caggcgcttg ccaaatgggg ccttaattac
      601 gttattgacg attatctgcc acgcaagctc gccaacgaga gcagtttcct gccatagaac
      661 cgcggaggct tgcagaccac gatgccgcga cgtgacgtgg caccaatcgc tcgtgcgaat
      721 ttgccagaat gtagcgttac tggcgtctct cggcacatca gcgccagcag cggcaaccgg
      781 taactccaac acgcctatgc tcacaaaaac aaattgtcag aaatgggtca tcaacggaca
      841 tcccgcgatg tccgcatcac gtccgctcta ccgccgataa ctgacatgcg ttgaatggaa
      901 tggcactgca gtttgccaga gccgaatgtg cggcaatcaa tcgccctacg attgtcaggc
      961 ttagctgatg ttcaggcgct gggcgctttc cgaccgacta tttccgcaag cggcgcgagc
     1021 gagtctgaaa ataattctgg agctgagcaa cctcgcatcg tacgaaaatc tggcacgcgt
     1081 cagaaaaagc gatcgtgcaa gtcgatgtcg tgactaagcg gcaaagtacg caaaaaaaca
     1141 atataacgag cagtacaaca tggagttttt ggtctccatc taagtaccta gaatcaatta
     1201 cagattcccg ctcagtcggc cttctccatc gtgggtgcaa tagcagattt tctaacgatt
     1261 tcagacattt aagctactat ttatggcgtc gttgagtccc agtggacgtc cggatcggcg
     1321 ccgatttttg tccctctggg ggcgcttctg ctctgccccc ttatagcgac catggatatg
     1381 attttcggaa tggacagcca cgttctcagt cacacccttt gtagtatgac tgctcacacc
     1441 gctttgccat ttcctgggcc tgcacgactt gcgctggggt cagacttttc gcaattctgt
     1501 cgcgactttc agtcgcccac ttacccgcat caccactaag ttttaatgcg gcaagattga
     1561 accacatatg ggcgcgcagg taatcttggg tgacacctcg gccgtccgca tacatgccac
     1621 cgaggacaat ctgcgcggat gcgattcctt gttcagccgc agaccgatac cacttcaccg
     1681 cttcccggta gtcctgggcg acaccttgtc cgcccgcgta catggcaccg agacttattt
     1741 gcgctttcgc gtctccttgt tcagcaagcg gccttaacag tcgcagagcg gtcgcat
//
LOCUS       DATCBB010000087         2900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_132555, whole genome shotgun sequence.
ACCESSION   DATCBB010000087 DATCBB010000000
VERSION     DATCBB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132555"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VII37_01020"
     CDS             <1..1030
                     /locus_tag="VII37_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5068326.1"
                     /translation="DLDKMLHHALPDGWDKDMPTFPADAKGVATRDSSGKVLNVLAKN
                     VPWLMGGAADLYPSTKTRLTFDGAGEFEGATRGGRNMHFGIREHAMAAISNGMALCKI
                     RTFASTFFIFTDYCKPAIRLSAIMELPVIYIFTHDSIGVGEDGPTHQPIEQLITLRAV
                     PGLVVLRPADANEVIEAWRVIMQLKKEPAVLVLTRQALPTVDRSKYASASGVAKGAYI
                     LAEAQGGKPEAILMGTGSEVSLCLAAYETLTSQGVKVRVVSMPSWDIFEHQSREYRDS
                     VLPPDVTARVSVEQASILGWDRYVGSKGKIIGMHTFGASAPLKELQKKFGFEPNAVVA
                     AVKEVIGK"
     gene            1037..1504
                     /gene="rpiB"
                     /locus_tag="VII37_01025"
     CDS             1037..1504
                     /gene="rpiB"
                     /locus_tag="VII37_01025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY5068327.1"
                     /translation="MKIVIGSDHAGFQLKAAMGDLLRSLNQEVLDVGAFNENPSDYPD
                     FAEAVGRAILDRKAERGVLICGSGVGASVAANKLTGIRAAVCHDSYSAHQGVEHDDMN
                     ILVLGSRIIGVKLAEDLVKAYLAAKFTNEDRHVRRLAKIKALESKFAVSSSAR"
     gene            1573..2745
                     /gene="tal"
                     /locus_tag="VII37_01030"
     CDS             1573..2745
                     /gene="tal"
                     /locus_tag="VII37_01030"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY5068328.1"
                     /translation="MTTSVKTSDTEVNKSEKNPLKGLLDYGQSPWMDYIRRDLLTGGE
                     LKRLIHDDGLRGMTSNPSIFAKAITGSKDYSDILESADAKKLNATQLYEKIAVRDVQD
                     AADIFRPVYDESRRRDGYVSLEVSPALALEADKTLEEARRLWKMVNRPNVMIKVPGTK
                     ECVPVIQQLLEEGININITLLFAQSAYEKVAEAFIAALEARAAKNQDIAHIASVASFF
                     VSRIDTLADSLIDEKLKSASDGGQKALLNGLKGKVAIANAKVTYKKYQELFSGPRWNA
                     LKAKGAQTQRLLWASTSTKNKAYRDVIYVEELIGADTVDTIPPATFDAFRDHGKLRPS
                     LTENVDQAVKTMSDLEKAGISMKEITDKLLVDGIKLFADAFNELLEATGKTAGVRA"
BASE COUNT          699 a          704 c          883 g          614 t
ORIGIN      
        1 agatctggac aaaatgctgc accacgcttt gccggatggc tgggataagg atatgccgac
       61 ttttccggcg gacgccaaag gcgtggccac gcgcgattct tccggcaaag ttctgaatgt
      121 gcttgctaaa aatgtgccct ggctgatggg cggagctgcc gatctctatc cttccaccaa
      181 aacgcgcctg acgtttgacg gcgcaggaga attcgaaggt gccactcgcg ggggccgcaa
      241 catgcatttt ggtattcgcg agcacgccat ggcggcgatc tcgaatggaa tggcgctttg
      301 caagattcgt acgtttgcgt cgacgttttt tatttttacg gattactgca aaccggcgat
      361 tcggctcagc gcgattatgg aattgccggt gatttatatt tttacgcacg attccattgg
      421 tgtgggcgaa gacggcccca cgcaccagcc gatcgaacaa ttaattacac tgcgtgcggt
      481 gccggggctg gtagtgctcc gtcccgcaga cgccaatgaa gttatcgagg cctggcgggt
      541 aatcatgcaa ttgaagaaag agccggcggt gctggtgctg actcgccagg cgctgcccac
      601 cgtcgaccgc agcaaatatg cttcagcgtc tggagttgcg aagggcgcgt acatcctggc
      661 tgaggcgcag ggcggcaaac cggaagcgat tctgatgggc accggaagcg aagtttcctt
      721 gtgcttggcc gcttatgaaa cactcaccag tcagggagta aaagtgcggg ttgtaagcat
      781 gccctcgtgg gatatttttg agcatcaatc gcgcgaatat cgcgatagcg tgctgccacc
      841 cgacgtcact gcgcgggttt cggtggaaca agcatccatt ctgggctggg atcggtacgt
      901 gggatcgaag ggcaaaatca tcggcatgca tacttttgga gcgtccgcgc ctttgaagga
      961 gctgcaaaag aaatttggat tcgaaccgaa cgcggtggtg gcagcagtga aggaagtaat
     1021 cggaaagtga gcggcaatga aaattgtcat tggttctgac cacgcgggat tccagttaaa
     1081 agccgccatg ggagatttgc tgcgatcttt gaaccaggag gttctggatg tcggggcgtt
     1141 taatgaaaac ccttccgatt atccggactt tgcggaggcg gtaggccggg caatactcga
     1201 ccgcaaagcg gaacgcgggg tgctgatctg cggcagcggt gtgggcgcaa gcgtagcggc
     1261 gaataaatta acaggcatac gtgcggcggt gtgtcacgac agttattcag cccatcaggg
     1321 cgtcgagcac gacgacatga atatcctggt actcggctcg cgcatcatcg gcgtgaagtt
     1381 ggcggaggat ctcgtgaagg cctatctcgc cgccaagttt acgaatgaag atcgccatgt
     1441 ccggcgcttg gccaaaataa aagctttgga atcgaagttt gctgtgagca gttcggcccg
     1501 gtaatagcgc gaaaagcgca tgaagcggtg caggcgtaca ataagagtca gggttagcaa
     1561 ggagagttac aaatgacgac ctcagtgaaa accagcgata cggaagttaa caagagcgag
     1621 aagaatccct tgaagggact cctcgattat ggccagtcgc cgtggatgga ctatatccgc
     1681 agagatttgc tgaccggcgg cgagctgaaa aggctgatac atgatgacgg gctgcggggc
     1741 atgacctcca acccttcgat attcgcgaaa gccattactg gcagcaaaga ttattccgat
     1801 attttggaat cggctgatgc caagaagctg aatgctacac agctgtacga aaaaatcgcg
     1861 gtgcgcgacg ttcaggatgc tgccgatatt ttccgtccgg tgtatgacga aagccgccgt
     1921 cgcgacggct atgtgagtct ggaagtatca ccggcattgg ctctggaagc agataagaca
     1981 cttgaggaag cgcggcgttt gtggaagatg gtgaatcgtc ccaacgtgat gatcaaagtg
     2041 cctggcacga aagaatgcgt cccggtgatt cagcaacttc tggaagaagg catcaatatc
     2101 aacattacgc tgctcttcgc gcaatccgct tatgagaagg ttgccgaggc attcattgcc
     2161 gcgctggaag cgcgtgccgc caagaatcag gacatcgcgc acatcgccag cgtggcgagt
     2221 ttctttgtga gccgtatcga tactttggcg gacagtctga ttgacgaaaa attgaagtcc
     2281 gcttccgatg gtggtcagaa agctttgctg aatggcctga aaggcaaagt ggccatcgcc
     2341 aacgccaaag tcacttataa aaagtatcag gagcttttca gcgggccgcg atggaacgct
     2401 ttgaaagcca agggcgcgca gacgcagcgg ctgctgtggg cgagcaccag cacgaagaac
     2461 aaggcctacc gggatgtgat ctatgtagag gaattgattg gcgccgacac ggtggacact
     2521 attcctcccg cgacgttcga tgcgtttcgc gaccacggca aattgcggcc gagcctgacg
     2581 gaaaatgttg accaggcggt caagaccatg tccgacctgg aaaaggccgg catctccatg
     2641 aaggaaatta ccgacaagct gttggtcgac ggcataaaac ttttcgctga cgcatttaat
     2701 gagcttctgg aggccaccgg caagaccgct ggcgtgcgcg cgtgagtcta aatcggcaga
     2761 cctacaaact gccggcttct ctttctggac cctttgccgc cgcgcgagat gattggaaaa
     2821 aaaacgaaaa ggttcggcgg ctgtggcagt gcgacgcttc gctttggagc ggcacagacg
     2881 aaggcaagtg gctggcttgg
//
LOCUS       DATCBB010000088         5126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_262546, whole genome shotgun sequence.
ACCESSION   DATCBB010000088 DATCBB010000000
VERSION     DATCBB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262546"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VII37_01035"
     CDS             <1..924
                     /locus_tag="VII37_01035"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY5068329.1"
                     /translation="KLQIAKRFLVKRQREATGLQEKNLSFTDDGLLHIIRHYTQEAGV
                     RNLEREIQNISRKMARKVVTEGPKAEVKAEIVPVNVNDYLGVLKYREFWLEKQNEIGL
                     TTGLAWTEVGGTVLTTEATLMEGKGRLTLTGKLGDVMQESAQAAMSYIRSRSASFGLP
                     KDFYRNIDIHVHVPEGAIPKDGPSAGITICNSIVSALTRIPVRRDVTMTGEITLRGKV
                     LPIGGVKEKLLAAHRMGLRTVLLPKDNEKDLAEIPQEILSALTIRFVETMDEVLQVAL
                     ERLIVPIEHPAVAPVAEPFVAGAEKDTSLTN"
     gene            1154..2407
                     /gene="rho"
                     /locus_tag="VII37_01040"
     CDS             1154..2407
                     /gene="rho"
                     /locus_tag="VII37_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5068330.1"
                     /translation="MSLAELKEKNITDLAKIAKELNIPGASGMRKQELIFQILRAQTE
                     KNGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQVR
                     PPKDGERYFALIKVEAVNFEDPEVARNKIFFDNLTPLYPQGRLKMETTKENMTGRVLD
                     LLCPVGKGQRGLIVAPPRTGKTMMLQSMANSITVNHPEVALIVLLIDERPEEVTDMQR
                     TVKGEVISSTFDEAPQRHIQVAEMVLEKAKRLVEHKRDVVVLLDSVTRLARAYNAVTP
                     PSGKVLSGGIDANALQRPRRFFAAARHVEEGGSLTIIATALIDTGSRMDDVIFEEFKG
                     TGNMEINLDRRLSDKRVFPAIDIQRSGTRKDEILLPPDELSRIWVLRKVLSPLSTVEA
                     MELLLSRLTKTKSNSEFLGSMSAPT"
     gene            complement(2476..4326)
                     /locus_tag="VII37_01045"
     CDS             complement(2476..4326)
                     /locus_tag="VII37_01045"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5068331.1"
                     /translation="MMRMLTLIVLNLGSGLLCMPMMLLAQGAPNQKSAVESSTTHNDA
                     TRKFRAYLEEDWKRWMEEYPEFATGTGFPGQNRRWSDDSPAGIEARKKHLQESAAALK
                     AFSRDSLTPAEQLNFDLYSDLLSTAEGGLQYGDDPLPFRNVVPQNNWMPINQMSGVQQ
                     GAAETISSMPNRSVADYEDILARLEALPASIAQQQELLQEGLKRGYTPPKLMLRDVPK
                     QIADLIPADPLASALLEPFKTFPAEIPESERTPLTNRAKEIYSARVLPAFQKLHDYIE
                     STYLPACRESIAATALPKGAEAYAYHVRWQTTTNLTPQQIHEIGLSEVKRIRAEMDKV
                     IASANFKGTFHEFTEFLRNDPRFYFEKPDDLVNGYRIIAKKIDPELAHLFGKLPRLPY
                     GVTPIPDFKAPSQTTAYYQAGAPSVGRPGYYFVNTYNLHARPKWEMEALSLHESVPGH
                     HLQISLAQEMENEPEFRKHVGYSAFVEGWALYCESLGEELGMYKDPYSKFGQLSYEMW
                     RAVRLVVDTGMHSQGWTREQAIQFFKDNTGKTDQDITVEVDRYIVWPGQALAYKIGQL
                     KIRELRTQAEKRQGAKFSVRAFHDAVLENGAVPLNVLSTHMEGWMATAHN"
     gene            complement(4323..>5126)
                     /gene="cmk"
                     /locus_tag="VII37_01050"
     CDS             complement(4323..>5126)
                     /gene="cmk"
                     /locus_tag="VII37_01050"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY5068332.1"
                     /translation="QLRARRANVHNHWPSSEARHYSARASNHMQKLTIAIDGPAGSGK
                     SSLARRVATMLGYLYMDSGAMYRAVALKALRRKLPLNDERALSAIARETHVELKRPSA
                     AQEAAGAKNCVFLDGLEVTDEIRTEEVAQAASRLATIRDVREVLVAEQQRTGAGGGIV
                     MEGRDIGTVVFPAAQLKIFLDASPEVRAERRWKEHQEKGHQVSLQEVVEEVRERDRRD
                     RERKVSPLVRAADAVLVDNTAMGIEETARLIVMLVKERETKTAKAGRAE"
BASE COUNT         1112 a         1409 c         1397 g         1208 t
ORIGIN      
        1 aagctgcaga tcgccaagcg gtttctagtt aagcgccaac gcgaggccac tggtcttcaa
       61 gaaaagaatc tgtcgtttac ggacgatggc ctgctgcaca tcatccggca ctacacccag
      121 gaagcgggag tgcgcaatct ggaacgcgag attcaaaaca tttcacgcaa gatggcgcgc
      181 aaagtggtca ccgagggtcc gaaggcagag gtaaaagcgg aaatcgtgcc tgtcaacgtt
      241 aacgactact tgggtgtttt gaaataccgc gaattctggc tggagaaaca gaatgaaatc
      301 ggcctgacta ccggactggc gtggaccgaa gtgggcggta cagttctgac cactgaagct
      361 accttgatgg aaggcaaagg ccggctcacc ctcaccggca aacttggcga cgttatgcag
      421 gaatccgcgc aagcggcgat gagttatatt cgttcacgct cggcttcttt tgggctgccg
      481 aaggattttt atcgcaacat cgatattcac gtgcacgtgc ccgaaggcgc gatacccaag
      541 gacgggcctt ccgcgggcat cacgatttgc aattcgatcg tgagcgcctt gacacgtatt
      601 ccagtgcgcc gcgatgtcac catgaccggt gaaatcactc tgcgtggaaa agttcttccc
      661 atcggcgggg tcaaagaaaa acttctggcc gctcatcgaa tgggcttgcg tacggtactg
      721 cttccgaagg acaatgagaa agacctggcg gaaataccgc aggagatcct ctccgctttg
      781 acgatccgat ttgttgaaac catggatgaa gttttgcagg tggctcttga acggctgatt
      841 gtgccgattg agcatcccgc agttgcaccc gtcgctgaac cgttcgtggc cggtgctgag
      901 aaagacacaa gtttgaccaa ctaaaagcgc tgatggagga cggttcgcgt tcgggctttg
      961 gcgaactgta atcctggtta agtagcgctt ccggttgaag atagtctggt ttgcctccct
     1021 ggccgagttc cgtgctttcg ccagtctgcg cgccacctcg tacacaatcc ggatttctct
     1081 aaggtcaatt ttcacccata aaagttaagg acatatgaaa gaaacaagcg tcgaaggaag
     1141 caaccagatg agcattagcc ttgccgagct caaagaaaaa aatattaccg atctcgccaa
     1201 gatcgccaag gaactcaata ttcccggcgc cagcggcatg cgcaaacagg agctgatttt
     1261 ccagatcctg cgcgcacaaa cagaaaagaa cgggctgatc ttctccgaag gcgtgctcga
     1321 atgcctgcct gacggattcg gctttctgcg cgcgcctgag tacaactatc ttcctggccc
     1381 tgacgatatt tacgtttcgc cctcgcagat tcggaaattc gatcttcgca ctggagacac
     1441 cgtctccggc caggttcggc cgcccaagga cggcgagcgc tacttcgcat tgatcaaagt
     1501 ggaagccgta aattttgaag atccggaagt cgcgcgcaac aaaatcttct tcgacaacct
     1561 cactccgctc tatccgcagg gccggctgaa gatggaaact accaaggaaa atatgaccgg
     1621 gcgggtgctg gacctgttgt gcccggtagg caagggccag cgcggtttga ttgtggctcc
     1681 tccgcgcact ggaaagacca tgatgctgca atccatggct aattccatca ctgtcaacca
     1741 cccggaagtt gcgcttatcg tgctgctcat cgatgagcgt cccgaagaag ttaccgacat
     1801 gcagcgtacc gttaagggcg aagtcatcag ctcgactttc gacgaagcgc cgcagcggca
     1861 tattcaagtt gcggaaatgg ttttggagaa agccaagcgt ctggtggagc acaagcgcga
     1921 cgtcgttgtt ctgctggaca gcgttacgcg ccttgctcgc gcttacaacg cggtaacacc
     1981 gccatcgggc aaggtgttat ccggcggtat tgatgctaac gccctgcagc gtccgcgtcg
     2041 cttctttgcc gctgcgcgcc acgtcgaaga gggcggctcg ctgaccatca ttgccactgc
     2101 gttgattgat acaggcagcc gcatggatga tgtcatcttc gaagagttca agggcaccgg
     2161 caacatggaa atcaatcttg accgacgttt gtccgataag cgcgtcttcc ctgctatcga
     2221 tattcagcgt tccggcacac gtaaggatga aatcctgctt ccaccagacg aactgagccg
     2281 gatttgggtg ctacgcaaag tgctcagccc gctatccacg gtggaagcga tggagttgtt
     2341 gctgagccgg ctcaccaaga ccaagtccaa ctccgaattc ctcggctcta tgtccgcacc
     2401 tacgtagatt tgtcggaaaa ataaacagag aattgctgct ttagaattac tccagccgcg
     2461 gtttggcctg cgcggttaat tatgcgcagt tgccatccag ccttccatgt gagtgctcaa
     2521 tacattgagg ggtactgcgc cgttttccag aacggcgtcg tggaatgcgc gtacgctgaa
     2581 ttttgctcct tggcgcttct ccgcttgggt acgtaattcg cggattttta gctgtccgat
     2641 cttgtatgcc agcgcctggc ccggccacac aatgtagcga tcaacttcga cggtaatatc
     2701 ctgatcagtc ttgccggtat tgtctttgaa aaattggatg gcctgttcgc gcgtccagcc
     2761 ttgcgagtgc ataccggtgt ccaccaccag ccgcactgcg cgccacattt cgtaactgag
     2821 ctgcccgaac tttgaatagg gatctttgta catgcccaat tcttcgccga ggctttcgca
     2881 gtagagtgcc cagccctcca caaatgcgga ataacctacg tgcttgcgaa attccggctc
     2941 gttttccatt tcttgcgcca aggaaatctg cagatgatga ccgggaacgg attcatgcag
     3001 cgaaagcgct tccatctccc atttcgggcg tgcgtgcaga ttgtaggtgt tcacgaagta
     3061 gtagcccggc cgtcccacac tcggcgctcc cgcttggtaa tacgcagtgg tttgcgaggg
     3121 tgccttgaaa tccgggatgg gcgttacgcc gtagggcaat cgcggaagct tgccaaacaa
     3181 gtgtgcgagt tctgggtcga tttttttggc gatgatgcga taaccgttga cgagatcatc
     3241 gggcttctca aaataaaaac gcggatcgtt gcgtagaaat tcggtgaact cgtggaaggt
     3301 ccctttgaaa ttggcggaag caattacttt atccatctcc gcgcggatgc gcttcacttc
     3361 cgacaatcca atttcgtgaa tctgttgcgg agtgagattt gtcgtggttt gccagcgcac
     3421 gtggtaagcg taagcttccg ctcctttagg aagcgccgtg gctgctatgg attcgcgaca
     3481 agcaggcaaa taggtggatt cgatgtaatc gtgcaatttc tgaaaagcgg gcaacacgcg
     3541 cgcgctgtaa atttctttcg cgcggttggt gagtggcgtg cgctcggatt cggggatttc
     3601 agcaggaaaa gtcttgaatg gctccagcaa agcgctcgcc agcgggtctg caggaatcaa
     3661 atcagcaatt tgtttcggga catcacgcag catcagcttc ggcggcgtgt atccgcgttt
     3721 cagaccctct tgcaacaatt cttgttgctg cgcgatggat gccggaaggg cttcgagccg
     3781 tgccaaaatg tcctcataat cggccactga cctgtttggc attgaggaaa tggtttcggc
     3841 ggcgccttgc tggacgccgc tcatctgatt tatgggcatc cagttgttct gtggcactac
     3901 attgcgaaat ggtagtgggt catcaccata ctgcaggcct ccttccgcgg tgctcagcaa
     3961 atcggaatac aaatcgaaat taagctgctc ggcgggcgtc agggagtcgc gcgaaaatgc
     4021 ttttaacgcg gccgcgctct cttgcaaatg tttcttgcgt gcttcaatcc ccgcgggtga
     4081 gtcatcgctc caccgtcggt tctgacctgg aaaacccgta ccggttgcaa attccggata
     4141 ctcttccatc cagcgcttcc agtcttcttc gagataagcg cggaatttcc gagtggcgtc
     4201 gttgtgagtg gtgctgcttt ccacggcgct tttttggttt ggcgcacctt gcgcgagcaa
     4261 catcatcggc atgcagagca agccgctacc gagattcagc actatcaagg tgagcatgcg
     4321 catcattcgg ccctcccggc ttttgcggtc ttggtttcgc gttcctttac cagcatcacg
     4381 atgagccgcg cggtttcttc gatacccata gcggtgttgt ctaccaacac cgcatccgca
     4441 gcgcgaacca gtggcgacac ttttctctcc cgatcacggc gatcgcgttc gcgaacttct
     4501 tccaccactt cctgcaacga aacttggtgg cctttttcct gatgctcttt ccagcgccgt
     4561 tctgcgcgga cttccggaga agcgtcgaga aaaatcttca gctgcgctgc tgggaatacc
     4621 accgtgccaa tgtcgcggcc ctccattaca attccgccgc cggctccggt acgctgctgc
     4681 tcagcaacca gtacttcacg cacgtcccgg atggtggcca aacgcgaagc cgcctgcgcg
     4741 acttcctcgg tgcgaatctc atccgtcact tcgaggccgt ccagaaaaac gcagttcttt
     4801 gcaccggctg cttcctgcgc tgcgctcggc cgcttcaact caacatgagt ttcgcgcgcg
     4861 atggcgctga gagctcgttc atcgttaagc ggcaatttcc tgcgcaatgc tttcagcgcc
     4921 accgcgcggt acattgcgcc gctatccatg tataaatagc ccaacatggt ggccacgcgg
     4981 cgggccaagc tacttttgcc cgagccggcc gggccatcaa tggcgatggt tagtttttgc
     5041 atgtggttgg aagcgcgtgc actataatgc cgcgcttcag atgaaggcca atggttatga
     5101 acatttgcgc gccgcgctcg caattg
//
LOCUS       DATCBB010000089         1634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_132570, whole genome shotgun sequence.
ACCESSION   DATCBB010000089 DATCBB010000000
VERSION     DATCBB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132570"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..909)
                     /locus_tag="VII37_01055"
     CDS             complement(118..909)
                     /locus_tag="VII37_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5068333.1"
                     /translation="MNFSGKVAFITGAGSPLGIGFATARLLAQQGAKIAITSTTDRIK
                     ERARELATENADVFALPADLRDHESTQMLVREVLARYGRIDILVNNAGMTQISNPDET
                     LSRSLVELTEADWDYSIGLNLKTTFNVTKAVLPSMLAANYGRIVNVSSVTGPLVSNPR
                     ATAYSTSKAGMIGLTRSLAMEVARNGITVNAVAPGWIETASSTAEEIIAGKNTPLGRP
                     GRPDEVAAAIAFLACESASYITGQMIVVDGGNTIQEYKGPSDSYY"
     gene            988..>1634
                     /locus_tag="VII37_01060"
     CDS             988..>1634
                     /locus_tag="VII37_01060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5068334.1"
                     /translation="MPLPPGTKLGPYEISAVLGAGAMGEVYRAKDTRLGRDVAIKVLP
                     GALAQNPERLQRFEQEARVLSAMNHPNLLAIFDVGSEDGLQYLVSELLEGKSLRERLN
                     DGALSQRKVVEYCTKIASGLAAAHEKGIVHRDLKPENVFVTNDEHVKILDFGLAKYAN
                     EAALTGATVTMTSAGTSPGTVMGTVGYMSPEQVRGEVADSRSDIFSLGAVLFEMAT"
BASE COUNT          358 a          465 c          470 g          341 t
ORIGIN      
        1 cgacctaaac gaagctaccc aactcgccgc gcaaatccca ccagctcgcg tgggcagcat
       61 cgaggtgcgc ccagtcaggg aactcacaag cacaagtgaa cgaagacagt tgaactctca
      121 gtagtaagaa tcggacggcc ctttgtattc ctggatagta ttgccgccat cgacaacgat
      181 catctgtccg gtgatgtagg acgcggattc gcacgcgaga aacgcgatag cagcggcgac
      241 ttcgtcagga cggccaggtc ggccgagcgg cgtattttta ccagcgatga tttcctcagc
      301 ggtagaggac gcggtttcaa tccagccggg agccacagcg ttcacggtga ttccgttgcg
      361 cgccacctcc attgccaggc tgcgggtcag cccgatcatg ccagctttgg acgtactgta
      421 agcggttgct cgcggattgc taaccagcgg accggtgacc gaggaaacat tcacgatgcg
      481 gccatagttt gcagccagca tggacggtag tacggctttc gtgacgttaa aagtggtctt
      541 caggttcagc ccgatgctgt aatcccagtc cgcttcggta agttccacca gagaacgcga
      601 gagggtctca tcaggattgc tgatttgcgt catgccggca ttgtttacca ggatatcgat
      661 gcggccatag cgcgcgagga cttcccgaac cagcatttga gtgctctcgt gatcgcgcag
      721 gtccgcgggc aaggcaaaaa catcggcgtt ctccgtggcg agttcgcgag ccctctcttt
      781 gatcctatcg gtggtggaag tgatggcgat ttttgcccct tgttgggcca gcagacgagc
      841 agtagcgaaa cctattccta gcggactgcc cgcaccagtg atgaacgcga cttttccgga
      901 aaaattcatt ttctccactc cctcatggat ctgcctgtca gcaacgctac cgctgaaact
      961 tttgctcaga gtataatccc cgcatagatg ccgcttcctc ccggaacaaa actgggccct
     1021 tatgaaattt ccgctgtgct cggcgcgggc gccatgggcg aagtgtatcg cgctaaggac
     1081 acgcgcctcg gccgcgacgt agccatcaag gtattgcccg gcgcactggc acagaacccc
     1141 gagcgcttgc aaaggtttga gcaggaagct cgcgtactga gcgcgatgaa tcatcccaac
     1201 ctcctcgcaa tttttgatgt ggggagtgag gacggcctgc agtatctggt gtccgagttg
     1261 ctcgaaggaa aaagcttgcg cgagcgcctg aacgacggcg ctctgtcaca gcgcaaagtg
     1321 gtggagtatt gcaccaagat cgccagcgga ctggccgccg cgcatgaaaa aggcatcgtc
     1381 catcgcgatc tgaagcccga aaatgttttt gtcactaacg acgagcatgt aaaaattctc
     1441 gattttggcc tggcgaaata cgccaatgaa gcggcactca ccggcgccac cgtaaccatg
     1501 acttccgcgg gaacttcgcc cggcacggtc atgggcaccg tgggctacat gtcgccggaa
     1561 caagtgcgtg gcgaagtggc cgactcgcgc tccgacattt tcagtttggg cgcggtgctc
     1621 tttgagatgg ccac
//
LOCUS       DATCBB010000090         2735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_132579, whole genome shotgun sequence.
ACCESSION   DATCBB010000090 DATCBB010000000
VERSION     DATCBB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132579"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..2329)
                     /locus_tag="VII37_01065"
     CDS             complement(197..2329)
                     /locus_tag="VII37_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY5068335.1"
                     /translation="MSTQPENRLKDSASPYLRSAAHQPIDWYEWGDAAFERAKADDKP
                     MLLDIGAVWCHWCHVIDRESYENPVIAKIISDNFVAVKVDRDERPDVDSRYQSAISSI
                     SGQGGWPLTGFLLPDGRPFFGGTYFPPEDQMGRPGFRRVLLAVAESYRSKRAELERAA
                     SSLAEAMAQAEVFTGARAAFNPAVVETQIASITQLFDIKNGGFGSAPKFPHASAMDLV
                     LERYQQTGEKHLLAMAESTLEKMARGGVYDQLAGGFHRYSVDERWLVPHFEKMSYDNS
                     ELLRNYLHGWQVTQNSFLRQIAEGIIAWVNEVLSDQENGGFYASQDADYSLDDDGDYF
                     TWTLEELRGALSPEEARIMEVHYDVEEHGEMHHNPAKNVLWIARDAEDIAAQLGLEEA
                     AVVATIAEAKAKMLAARMLRPTPYIDQTMYVSWNAMFVSAYLEAARVLDGLVAESCRR
                     FALKTLDRMLKETWSEARGFAHRVGGPALDGSLDDQIFGVLALLGAYETTLEPRYFEA
                     AKKTMDLAISRYGDPDGGGFFDRPSDAAPMGGLDVRRKPFQDSPTPGTNSMAAIALIR
                     LQAFSGEEIYRVSVQKTLEAFAGIAPRYGLFAATYGLAATLFVHHPLQIVITGAADDW
                     VAQSLELAAHRVFRFGKAVLRVTPGTKLAALAGALKETLPHLPPDKPVAMVCAGQSCL
                     PPATDAGQLTALLQNVHQSGAAGVAAPA"
     gene            complement(2397..>2735)
                     /locus_tag="VII37_01070"
     CDS             complement(2397..>2735)
                     /locus_tag="VII37_01070"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY5068336.1"
                     /translation="GLESDLSAVSSRIAMMGGVVVPYEKLAAVRLQHDQLAETFNSAL
                     TQIIEMGCVIKDLDVGLLDFPAIIDNQDVYLCWKLGEDRIRFYHRQDEGFAGRKPLDP
                     RDLGSSDIVQ"
BASE COUNT          630 a          835 c          684 g          586 t
ORIGIN      
        1 tggcgtatgg catcatcgag cattccggcg agcactatgg acaattggtg gtgtattacc
       61 gcttgtctgg attggtgccg ccagaatccc gtcccaagaa gtagtcttat cccaggaagt
      121 acagccaaaa actttggaaa caaatctaga cggactcgac aatgtgtcgg gtccgtttat
      181 tttttaagct gctattctac gcgggcgccg cgacgcccgc cgcaccgctc tggtgaacgt
      241 tctgcagcaa ggctgttaac tgcccggcgt ccgtcgccgg cggtaaacaa ctttgcccag
      301 cgcacaccat cgcaaccggt ttgtccggtg gaagatgcgg aagagtttct ttcagcgcgc
      361 ctgccagagc tgcgagtttt gttcccggag ttacgcgcag aacagctttg ccaaagcgaa
      421 atacccggtg cgcggccaat tcgagcgatt gtgccaccca atcgtcagcc gcgccggtaa
      481 taacaatttg cagcgggtga tgcacaaaaa gcgtggctgc aagtccgtac gttgccgcaa
      541 acagcccgta acgcggtgcg atacccgcaa aagcttccag tgttttttgc acggacacgc
      601 gataaatttc ttcgccgctg aatgcctgaa gacgaatcag agcaatagcc gccatggaat
      661 tagtaccggg cgtgggcgaa tcctgaaaag gttttcgccg cacatccagc ccacccatcg
      721 gcgctgcatc gctgggacga tcaaaaaaac cgccgccatc gggatcacca tagcgtgaga
      781 tggccaggtc catagttttt ttcgcagctt cgaaatagcg cggttccagc gtcgtttcat
      841 acgctcctag caacgccaac acgccaaaaa tctgatcgtc gagtgagcca tccaacgccg
      901 ggccgccgac gcggtgagca aaaccgcgcg cttcgctcca ggtttccttg agcatgcggt
      961 ccagagtttt cagtgcaaac cggcggcaac tttcagctac cagcccgtcc aaaacccgag
     1021 ctgcctccag ataagcggat acaaacatcg cgttccacga aacgtacatg gtttgatcta
     1081 tgtaaggtgt cggccgcagc atccgcgccg ccagcatttt tgcttttgcc tcagcgatgg
     1141 tcgcgactac cgccgcttct tccagcccca actgcgcggc tatatcctcc gcatcgcggg
     1201 caatccacaa aacatttttt gcgggattgt ggtgcatctc cccgtgctct tcgacgtcgt
     1261 aatgcacctc catgatgcga gcttcctcgg ggctcaacgc gccacgcagc tcctccaggg
     1321 tccacgtaaa ataatcgcca tcgtcatcca gcgagtaatc ggcgtcctgg ctcgcgtaaa
     1381 acccgccatt ttcctggtca gacaacactt cgttaaccca cgcaatgatg ccttccgcga
     1441 tctgtcgcaa aaatgaattt tgcgtgacct gccagccatg caaataattt cgcaacagct
     1501 cggaattgtc gtaactcatc ttttcgaaat gcggcaccaa ccaccgttcg tccaccgagt
     1561 agcgatgaaa gcccccagca agctgatcgt aaacgccgcc gcgcgccatc ttctccagcg
     1621 tgctttccgc catggctagc aaatgtttct cgccagtttg ttgatagcgt tccaggacca
     1681 gatccattgc cgaagcgtga ggaaatttcg gcgcgctgcc gaatccgcca ttcttgatgt
     1741 cgaagagctg cgtgatcgac gcgatttgtg tttccaccac cgccggatta aatgccgcgc
     1801 gagcgccggt gaatacttcc gcctgcgcca tcgcctcggc taatgaactc gcagcccgct
     1861 ccaactcagc gcgtttgctg cgatacgatt cggccaccgc aagcagaaca cgccgaaatc
     1921 ccgggcgccc catctgatct tccgggggaa aataagtacc gccaaaaaac ggacggccat
     1981 cgggcagcag aaatcccgtt agcggccaac cgccctgacc cgagatggaa ctgatagccg
     2041 actgataacg cgaatccaca tccggccgct catcgcggtc cacttttacc gcgacaaaat
     2101 tgtcactaat gattttggca atgacgggat tttcgtacga ttcgcggtcg atgacatggc
     2161 accagtggca ccacaccgcc ccaatatcca gcagcatcgg tttgtcatcg gccttggcgc
     2221 gttcgaatgc cgcatcgccc cactcgtacc aatcgatagg ctgatgcgcg gcagagcgca
     2281 gataagggct ggcggaatct ttcagacgat tttcaggttg cgtcgacaag aagttccttt
     2341 tatcgaggat tggagcgttt ttttagcgtt tgtgccgggc gatttagccc gaccgcctac
     2401 tgaacgatgt cactcgaacc aagatcacgc gggtccagag gctttcgccc agcaaagcct
     2461 tcgtcctggc ggtgataaaa acgaatgcga tcctcgccga gcttccagca cagataaaca
     2521 tcctgattat cgataatcgc cggaaagtcc aataacccca catccagatc cttaatcaca
     2581 catcccattt caataatctg cgtgagtgcg gaattaaagg tctccgccaa ttggtcatgt
     2641 tgcaagcgca ccgccgcgag cttttcgtac ggcacgacta cgccgcccat catcgcgata
     2701 cgcgaagaaa ccgcggacaa atcactctcc aggcc
//
LOCUS       DATCBB010000091         1561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_2735, whole genome shotgun sequence.
ACCESSION   DATCBB010000091 DATCBB010000000
VERSION     DATCBB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..>1561
                     /locus_tag="VII37_01075"
     CDS             436..>1561
                     /locus_tag="VII37_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068337.1"
                     /translation="MGTFRFRRCSNAVFAALIATLCMAPGARSQGGEPPYFAIRGAKV
                     VTVSGRTTENSTIIISRGIIAAVGADAKIPDEAWIVDGKGLTVYPGLIDSYTDVGLVA
                     TPATATPGATDTASRPPQTQIVARSPEDRPGTTPWRNAADEVNLSDKRIETWRNGGFT
                     TVITAPKGGFFPGLAAVLDLAGDRPGDFVVKSPAAIPLSLRPTGGFNSGFPDSLMGVL
                     AYVRQVLLDTDWSTRAQAAYENNSRVDRPHYDRTEASLAAALQKHVVVLIPANNSVEI
                     RRSLELADRWKVSGVIYGAQMAYEDAAEISAKKLPVLVNAKWPEAEKDADPEDKPSLR
                     ELRFRDRAPSTPAALAKANVKFAFYSGGLTAPKDVLKAVKK"
BASE COUNT          336 a          428 c          442 g          355 t
ORIGIN      
        1 gctcgagttt cagcaatgcg ggatatagcg tgccgtagtt caccgccagg aggtcaccgc
       61 tggtctgctc gatgcgccgc gcgatcccgt aaccgtgcaa ggggccaagc gcttcgagcg
      121 tcttgaggat catcagtgcc agcgtgcctt gccaaacgtc aactttttcc cccattaacg
      181 cgtgtccgcc ttttttgacc ctatgggatg cccatagtaa acccgctgcc ctcctatggg
      241 aagccaatag taaagacgct cgagatgcca gtttgtttct gcgtgcgact cgttattttt
      301 aatttttgac gatatccaat ctcaaggctt tcttgactct tcggcaactt gccgttagcc
      361 tcgtgcgttc aattagaaat ggcgacggtt atctctcgct atcgaaaaat tcttcctcac
      421 ggcaaggagc gtttcatggg cacctttcgg tttcgtcgat gctccaacgc agtattcgca
      481 gcgctaatcg ctacgttatg catggcgccg ggggcccgta gtcagggagg ggaaccgcct
      541 tacttcgcga ttcgcggagc gaaggtcgtt acggtgtcag ggcgaacgac ggaaaattcc
      601 accatcataa tttcgcgcgg aattattgct gccgttggag cggacgccaa aatccccgat
      661 gaagcgtgga tcgtcgatgg aaaagggcta accgtatatc ccggactgat cgattcgtat
      721 acagatgtgg gcttggtggc gacaccggcc actgccacac ctggcgccac agataccgcg
      781 tcgcgacctc cacaaacgca aattgttgcg cgcagtcctg aggatcggcc gggcacaaca
      841 ccttggcgca acgctgcgga cgaagtgaat ctcagcgaca agcgtattga gacctggcgc
      901 aatggaggtt ttacaaccgt catcaccgcg cctaagggcg gtttcttccc cggcttggcc
      961 gcggtgcttg atcttgcagg agatcgtcct ggagacttcg tcgtgaaatc tcctgcggcg
     1021 attccgctaa gtctgcgccc taccggcgga ttcaacagtg gcttcccgga ttcgctgatg
     1081 ggggtgctcg cgtacgtgcg tcaggtgctc ctggacaccg attggagcac ccgggcgcaa
     1141 gccgcttacg aaaataattc gcgtgtagat cggccgcatt atgaccgcac cgaagcgtcc
     1201 ctcgccgctg cactgcaaaa gcatgttgtg gtgctcatcc cggcaaacaa tagcgtggag
     1261 attcgacgtt cattagagct ggcggataga tggaaggtca gcggggtgat ttacggggcc
     1321 cagatggctt atgaagacgc tgcggaaatc agcgcaaaaa aacttcctgt gttggtgaac
     1381 gccaaatggc cggaagcgga gaaagacgcc gaccctgaag acaagccctc tttgcgcgag
     1441 ttgcgttttc gcgatcgcgc tccatccaca ccggcggcgc ttgccaaggc taatgtaaaa
     1501 ttcgcatttt attccggcgg gttaactgcg ccgaaggatg ttttgaaagc tgtgaaaaaa
     1561 t
//
LOCUS       DATCBB010000092         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_35405, whole genome shotgun sequence.
ACCESSION   DATCBB010000092 DATCBB010000000
VERSION     DATCBB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35405"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VII37_01080"
     CDS             complement(<1..485)
                     /locus_tag="VII37_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5068338.1"
                     /translation="MAEENSARSAPRAAAGSTVSSPPSVENTPPGRPYRAPKNRNKTV
                     ILVVVILIVLVGGFFLWRYFSTYESTDDAQVDVHLYPVSARISGYVIKVNVNDNQWVQ
                     QGTVLAEIDPKDFEVAVAQAQANLANAEATAQSLNITVPITSTNASSQLRSTASGIEN
                     S"
     gene            complement(488..1753)
                     /locus_tag="VII37_01085"
     CDS             complement(488..1753)
                     /locus_tag="VII37_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5068339.1"
                     /translation="MPLSLQEAIDRGLKQNLGLLLSNTDVRSARGQRWEQLSALLPHA
                     TLSPYVEVSKINLGELGLTSLPGAAALPASVGPFSYFDSRATVTQSVFDWKFINAARA
                     ASQNVKSAEYSYKDARDLVVLAVGFVYLQAIADEARIETSEAQVKTAQALFDQATDQV
                     NSGTSPAIDGLRAKVELQTRQQQLIQARNNFAIQKLTVARVIGLAPGQEIELTDKSPY
                     QPIESITVDEALKRAYDSRSDYQGAMAEVRAAEFYRRAAKAGYLPSLSFNADYGVAGA
                     HPSTATQVYDARGTLTIPIFQGGAVHGDVLQADARLEQSRDRLDNLRAQIDSDVRTAL
                     LNLQSSQEQVNVARSNVDLSEETLVQSRDRFVAGVADTVEVVQSQEAVAAAHEQYIAS
                     LYSYNYAKISLARALGMAEAGVKEYFKGK"
BASE COUNT          500 a          668 c          595 g          536 t
ORIGIN      
        1 tggctgtttt caatacccga tgcggtggac cgcagttgac tggaagcgtt cgtcgaagta
       61 atcggcaccg taatattcaa cgattgcgcg gtggcttccg cattggcgag attggcttgc
      121 gcctgtgcaa ctgcaacctc aaagtccttg gggtcgatct ctgcgagcac cgtaccctgc
      181 tgcacccatt ggttgtcgtt cacgttgacc tttattacgt agccggaaat tcgggcgcta
      241 acagggtaaa ggtgcacatc tacctgcgca tcgtcagtag attcgtaagt gctgaagtag
      301 cgccacagaa aaaagcctcc caccaaaaca atcaagatca ccactaccag aattacagtt
      361 ttgttgcgat tctttggcgc cctgtatgga cgacctggag gtgtattctc taccgaaggg
      421 ggcgaactaa cggtagaccc ggctgccgct cgcggcgccg aacgagctga attctcttcc
      481 gccatattta ttttcctttg aaatattctt ttacgcctgc ctcggccatt ccgagagctc
      541 gtgccagaga aattttggcg taattgtagc tgtagagact ggcgatgtat tgctcgtgcg
      601 cggcggccac ggcttcttgc gattgcacca cttccacggt atcggcaacg cccgccacga
      661 agcgatcacg cgactgcacc agcgtctctt ccgataaatc aacgttgctg cgtgccacat
      721 tgacttgctc ttgtgacgac tgcaggttca gcaacgccgt gcgaacatcg gagtcaatct
      781 gcgcgcgtaa attatccagg cggtcacggc tttgctctag cctggcatcg gcctgcaaaa
      841 catcaccgtg aaccgcgccg ccctgaaaaa ttggaatcgt gagcgttcca cgcgcgtcgt
      901 atacctgtgt agctgtcgag ggatgcgctc ccgccacgcc ataatcagca ttgaacgaga
      961 gcgacggcag ataccctgct ttggcagcgc ggcgataaaa ttctgccgct cgcacctccg
     1021 ccatggcccc ttgataatca gagcgcgaat cgtaagcgcg cttcagggct tcatccacag
     1081 tgatgctttc gatcggctgg tatggagatt tatccgttag ctcgatttcc tgtccaggcg
     1141 ccagcccaat cactctggcg acggtaagtt tctgtatggc gaagttgttc ctagcttgaa
     1201 tgagttgctg ctgccgcgtc tgcagttcta ccttggctcg caagccgtca atggccggcg
     1261 aggtgccgga attcacttga tccgtcgctt gatcgaatag cgcttgcgcc gtcttgacct
     1321 gcgcctccga tgtttcgatg cgcgcttcat ccgcgatggc ttgcagatat acaaatccaa
     1381 cagcgagcac aaccagatcg cgagcatcct tgtaggagta ttccgctgat ttaacattct
     1441 ggctggctgc gcgcgcggcg ttgatgaatt tccagtcgaa caccgactgc gtcaccgtgg
     1501 cacgcgaatc aaaatatgaa aacgggccca ctgacgccgg gagagcagcg gcgccaggta
     1561 aacttgtaag acccagttcg ccgagattga ttttcgatac ctctacgtac ggcgacaatg
     1621 tcgcgtgcgg taacagcgcg cttaattgtt cccaacgctg tccgcgagcg gaccgaacat
     1681 cggtattcga gagcaacagc ccaagattct gtttcaatcc gcgatcgatg gcttcctgca
     1741 aagagagcgg caaaacgcct ggaaccagct tcgcagggac cgacccagag aacgagctac
     1801 taccagccga cccgggtata gccgtgcttg gcgccgttgc actgggaacg gacgaggttt
     1861 gcgtttgcgc cctcaacgaa tcggacagca aaactccggt cagcaggaat gcgcagccca
     1921 ggttgcaaat catcctcaag gccacctccc ttgcgcaaag cttttcgaca gcgcggggcc
     1981 aacatcctat tcccacttgc cattcctaac aatggctatc tgcatcaaca cggaagaact
     2041 ggtcgtccac agtcacaaca gcacacaatc gatatcgacc gcgggggtgc ttctggatac
     2101 ttcgatgact ctcgacgaca tcgcaaaatc gtggcctaca atccgattcc tgatgctcgc
     2161 gtctcgcgtc gctccacgtg tagaatggca gcgattctga ggagatattt atgaatctta
     2221 attacccttc tctcgtggtt tttgcttttc tctgcgcttc cagtgtctgc agttcgccca
     2281 ccgcatatgc tgccccacc
//
LOCUS       DATCBB010000093         1289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_295167, whole genome shotgun sequence.
ACCESSION   DATCBB010000093 DATCBB010000000
VERSION     DATCBB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295167"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VII37_01090"
     CDS             complement(<1..1287)
                     /locus_tag="VII37_01090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5068340.1"
                     /translation="MRILPELVLSLFGILVMVVDPLLDPDSSHESLGTIALTGALAAL
                     AATFWMARYPGVAFYNMVRVEAFSVYFHVLVIAIAVVAILSSFEYLKVQRIRAGEYYG
                     LILFGTVGMCLMSSAIELVLIFIALEISSISTYILAGFRRRDASSSESSLKYFLLGSF
                     ATAFFLYGVALMFGATGSTNIDAISQVLRSGNVVVLAYVATALIFVGLGFKVASAPFH
                     VWTPDVYEGAPAPVVGLMSTGPKAAAFAVLLRVMFEANAPGRFWLIWVSAALSMTLGN
                     VGALVQNNVKRLLAYSSIAHAGYLLVAFAAEPQLGTSAAMFYAASYAAMNVGAFAVVG
                     HFANFGEKYVALEDYAGLGRRSPVLAATLTFFLMSLIGIPITGGFFAKFYVLSAALKS
                     NLVGLAIILVLNSAVGAYYYLRIIVMMYMREPRGDVP"
BASE COUNT          353 a          374 c          336 g          226 t
ORIGIN      
        1 cggcacgtct ccacgtggct cgcgcatgta catcatcacg ataattcgca ggtagtaata
       61 tgcgccgacc gcactattca gcaccagaat aattgccagg ccaaccagat tcgatttcaa
      121 agccgcgctc aatacgtaga acttcgcaaa gaacccgcca gtaataggaa ttccaatcaa
      181 ggacatcagg aagaatgtca gggtcgctgc cagtacgggc gaccgcctgc ccagtcccgc
      241 gtaatcctcg agcgcaacat acttttctcc aaagttggcg aaatggccta ccacggcgaa
      301 tgctcccacg ttcatggccg cataggaggc ggcataaaac atcgcggccg acgtgcccag
      361 ttgcggttcc gccgcgaatg ccaccagcaa atatccagcg tgtgcgatgg atgaatatgc
      421 gagcaggcgt ttaacgttat tctgcacgag cgcgcccaca tttccgaggg tcatggaaag
      481 ggcggcagac acccagatca gccagaagcg tcccggcgcg ttcgcttcaa acatgacgcg
      541 caacaacacc gcgaaggccg cagccttggg ccccgtcgac atcagtccta ctaccggggc
      601 cggcgctcct tcatagacat cgggagtcca tacatgaaat ggcgcggatg cgaccttaaa
      661 tcccaagcca acaaagatga gcgcggtcgc cacataggcc agaacaacga cgtttcctga
      721 gcgcagtacc tggctgatcg cgtcgatgtt cgtggagccg gttgcgccga acatcagcgc
      781 cacgccatag agaaaaaatg cggtggcaaa cgatcccagc aaaaaatatt tcaatgacga
      841 ttcgctactg gaggcgtcgc gccggcgaaa gcccgcgaga atgtaagtgg aaatcgacga
      901 aatttccagt gcgataaaga tgagtacaag ttcgatagcc gacgacatca ggcacattcc
      961 gactgtgcca aacaaaatca ggccgtaata ttcaccggcg cgaatgcgct gcaccttcaa
     1021 atattcaaag gagctgagaa tcgcgaccac ggcaattgct atcaccagca cgtgaaaata
     1081 gacgctgaat gcctcaacgc gaaccatgtt ataaaacgca actcctggat atcgcgccat
     1141 ccagaacgtc gcggccagag cggccaatgc ccctgtcaat gcgattgtgc ccagggattc
     1201 gtgactgctg tcaggatcaa ggagcggatc gacgaccatg accagtatgc cgaaaaggga
     1261 cagcaccaac tccggcagaa tgcgcatgt
//
LOCUS       DATCBB010000094         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_100413, whole genome shotgun sequence.
ACCESSION   DATCBB010000094 DATCBB010000000
VERSION     DATCBB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100413"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VII37_01095"
     CDS             <1..274
                     /locus_tag="VII37_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068341.1"
                     /translation="PWVEIDLVRINPIGSHMDADPETVVSVLREMRTAGKAIVGMKIL
                     GQGDLSGRQDEALKYALSLGLLDAFTIGAESKKHQDDLIRRIGAAA"
     gene            327..842
                     /locus_tag="VII37_01100"
     CDS             327..842
                     /locus_tag="VII37_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068342.1"
                     /translation="MVLSKEELISSLKNEVRLLLHLAGKIDKAKLNYRPTPRQRSTLE
                     LLQYMAIMAPTQVVAIKGGDFSRPTMMAIWGPAESASKAMNFEQTIAAIEQQGAQFDE
                     VFSGWTEADFRNELELFGQKSSRGAMLVNLVLGGFAAYRMQLFCYLKSCGREELNTVN
                     LWMGADSFTAP"
     gene            complement(908..1870)
                     /locus_tag="VII37_01105"
     CDS             complement(908..1870)
                     /locus_tag="VII37_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEY5068343.1"
                     /translation="MRAIRVSQFGGPEVLKLEEIPTPTPAAGQVLVHVRAAGVNPYEA
                     AQRAGTYAIKPPLPYTPGSDAAGVVESVGAAVTRVKAGDRVYTARTLSGSYAEYTLAQ
                     ENQVQPLPDDVEFAQGAGIWVPYGTAYHALFHSAQAHAGESVLIHGASGGVGTAAVQM
                     ARAIGLTIFGTAGTEPGLELIKREGASHAFNHTKAGYEDEILKATDGKGVDIILEMLA
                     NVNLAKDLKLLALNGRVIVIGNRGEITINPRELMTRRGSIRAFTLWAITEQETKEIYA
                     GLNAGLSNGTLLPVVGKEIPLAEAARAHKEILEPGSAGKIVLIP"
BASE COUNT          439 a          517 c          598 g          458 t
ORIGIN      
        1 accttgggtg gaaatcgatc tggtgcgtat caatccgatt ggctcgcaca tggacgccga
       61 tcctgaaacc gtcgtcagcg tattgcggga gatgcgcacg gcagggaagg ccatcgtggg
      121 gatgaaaatt ctggggcaag gagatttaag cggccgccag gatgaagcgt tgaaatacgc
      181 gttgtcgctg ggattgttgg atgcgtttac gattggcgcg gaaagtaaga agcaccagga
      241 tgatttgatc cgccgaatcg gcgcggcggc ctgacgagga aatgtagggc tgttactcca
      301 ttcttgatcc taaaatccga ggcgttatgg tcctgagcaa agaagaactg atctcctcac
      361 taaaaaacga ggtgcgcctg ctactacacc tggcaggaaa aatcgacaaa gcaaaactga
      421 attaccggcc aacgccgcgg cagcgcagca ccctggaact gctgcaatac atggccatca
      481 tggcgcccac acaggtggtg gctatcaagg gtggagattt ttcgcggcct acgatgatgg
      541 cgatttgggg gccagcggaa agcgcctcga aggcgatgaa ttttgagcag acgatcgcgg
      601 cgatcgaaca gcaaggggcg caattcgatg aggtattcag tggttggacg gaagctgatt
      661 ttcggaacga actggaattg ttcggtcaaa aatccagtcg tggagcgatg ctggtgaacc
      721 ttgtcttggg tggttttgcc gcgtaccgga tgcaactgtt ttgttatctc aagtcctgcg
      781 ggcgcgaaga gctgaacacc gtgaatttat ggatgggcgc ggactccttc accgcaccat
      841 aaacggaatt tccccgactg gctaagcgat cagaaatttc gaaaacctct tgctgcttac
      901 tgagcgatca gggaatcaaa acgatttttc ccgctgagcc gggctcgaga atttctttgt
      961 gggcgcgtgc ggcttccgcg agagggattt ctttgcccac cactgggaga agtgtgccgt
     1021 tggaaaggcc ggcgttcagc ccggcgtaaa tttctttggt ttcctgctcg gtaatggccc
     1081 acaaagtaaa ggcgcgaatg gatcctcgcc tggtcataag ctcacgaggg ttgatggtaa
     1141 tttcgccgcg attgccgatc acaatgacgc ggccgttgag ggccagcaac ttcaagtctt
     1201 tggcaagatt cacgttggcg agcatttcca gaatgatgtc aacgcctttg ccatcggtgg
     1261 ctttcaggat ttcgtcctcg taacctgcct tggtgtgatt gaatgcgtgg ctcgcgcctt
     1321 cacgtttgat gagctcaagg cccggctccg tgccggccgt cccgaaaatg gttagaccga
     1381 tcgcgcgtgc catttgcaca gcggcggtgc caacgcctcc acttgcgcca tgaatgagta
     1441 ccgattcgcc ggcgtgtgcc tgcgcggagt gaaaaagcgc gtgataggcg gtgccataag
     1501 gcacccatat tcctgcaccc tgcgcgaatt caacgtcgtc gggcagcggt tgaacctggt
     1561 tttcctgggc gagagtgtat tcggcatagg atccagacag cgtgcgagcg gtgtaaacgc
     1621 gatccccggc tttaacccta gtcacggcgg cacccacaga ctccaccact ccagcagcat
     1681 ccgagcctgg tgtgtacggc aatggcggct tgatcgcata ggttcccgcg cgctgggcag
     1741 cttcgtaagg gttgacgccg gcggcgcgca cgtgaacaag cacttgtccg gccgcgggag
     1801 tgggcgtcgg gatttcttcc agcttcaaaa cttccgggcc gccaaattga ctgacgcgaa
     1861 tcgctctcat accaggcctc caatgatttt gaacaataaa tttggtcgat tagaaatgtt
     1921 cgtcggcgac tacaggggtt ccgtcttttt tatcgagcaa tccggattcg cgagcccacg
     1981 cagtggtctg ccaattgtct ttgccttttt tc
//
LOCUS       DATCBB010000095         2427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_2862, whole genome shotgun sequence.
ACCESSION   DATCBB010000095 DATCBB010000000
VERSION     DATCBB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2862"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1028)
                     /gene="pgeF"
                     /locus_tag="VII37_01110"
     CDS             complement(93..1028)
                     /gene="pgeF"
                     /locus_tag="VII37_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY5068344.1"
                     /translation="MTPTKTKSVSRARTAKHRTLASHDGWKVRTSADGVGFLQVPAFA
                     AFPWLIHGFSTSQGGVSSLNGAKVLNLGFTEWDARENVQENRRAFQSALAADAFSLVS
                     LKQFHSDVVCGFSSAPKEPCSGDAAISNAPDLLLGIQTADCVPILLLDPKKRAVAAVH
                     AGWRGTLQRIVEKTIGRMKMEFKSNPGDLLAAIGPAIGGCCYEVGTEVAAAFHSQFTN
                     APEWFDELRTGDEPNPLQWLNQFPPGHQPPPKNVRLDLRKANRAQLLAAGLPPQNIFV
                     SDLCTACRPDLLFSYRKQGSESGRMMSVIGIRAEK"
     gene            1082..1459
                     /gene="acpS"
                     /locus_tag="VII37_01115"
     CDS             1082..1459
                     /gene="acpS"
                     /locus_tag="VII37_01115"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY5068345.1"
                     /translation="MIVGMGVDIAEVARIRGAIERRGEAFLRRIYTPGEIAYCERFKN
                     KFERYAGRFAVKEAAMKALGTGWSHGVRWVDLEVIREKSGRPTLVIRGEAGKIAARLG
                     VKNIVVSITHTEEQALAQVIFEG"
     gene            complement(1472..>2427)
                     /locus_tag="VII37_01120"
     CDS             complement(1472..>2427)
                     /locus_tag="VII37_01120"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEY5068346.1"
                     /translation="TRPTLKIQDGCDSHCAYCVIPQVRGKSRSLPLETVLQQVNQLYD
                     SGVKEIVLSGINIGTYGRDLNPRLSFHDLLRRILNETPLERLRVSSIEPMDVTTDLIE
                     FFAANDRMAPHFHMPLQSGADRILAAMHRWYRAEHYARRANLIREILPHAAIGADVIA
                     GFPHESDADHAATLAFIESLPLTYLHVFSFSKRPGTQAATLGHEVPARVIRNRARELR
                     ALAQRKSDAFRKDQIGRQLRVLTLRSAESRHDSASVDPLPHQESDYTPALSNNYLRVR
                     LRGHWPPNQWMNVRVQSVEKSDLIGEPVERISAELSFRAFA"
BASE COUNT          560 a          579 c          735 g          553 t
ORIGIN      
        1 cacaagttta atcacgctgt aaaaaaccca gcgattcgct cgagatttta gtttcaacga
       61 ttatttttcc gatgaattcc caatgctcaa acttattttt ctgctcgaat gcctatcact
      121 gacatcatgc ggccgctttc gctgccctgc ttgcgataac tgaaaagaag atcggggcgg
      181 caggcagtgc aaagatcact tacaaaaata ttttgcggag gcaggcctgc cgccagtaat
      241 tgtgcgcgat tggctttgcg caaatcgagg cgaacattct ttggcggcgg ttgatggccg
      301 ggaggaaact ggttgagcca ctgcagcgga ttgggctcgt caccggtgcg caattcgtcg
      361 aaccattccg gggcattggt aaattgggaa tggaacgccg cagccacttc cgtgccgact
      421 tcatagcagc atccaccaat ggctgggccg atggccgcca ataagtcgcc cggatttgat
      481 ttgaattcca ttttcatacg gccgatggtt ttttccacga tgcgctgaag cgtgccgcgc
      541 caaccggcgt gcacggcggc gacggcgcgt ttctttggat cgagcaataa aataggcacg
      601 cagtcggcgg tttgaattcc caggagaaga tctggagcat tggaaatagc ggcatccccg
      661 ctgcagggct ccttcggcgc ggaagaaaaa ccgcacacca cgtcggaatg aaattgcttt
      721 agcgaaacca gactaaaggc atcggcagca agggcggatt gaaaggctct gcggttttcc
      781 tgcacatttt cgcgagcatc ccattcggtg aatccgagat tcagcacttt ggcgccgttg
      841 agagaactga ctcctccttg agaggtgctg aagccgtgga ttaaccacgg aaacgcggcg
      901 aaggctggaa cttgaagaaa gcctacaccg tctgcagaag tccggacttt ccatccatcg
      961 tgcgacgcaa gcgtcctgtg tttggcagtt cgcgctctcg atacggattt tgtcttcgtg
     1021 ggcgtcatct taatggtccc agtataatcg tggtggaagt gaactgcttg cgggaggtat
     1081 tttgatcgtt gggatgggag tggacatcgc ggaggtggca cggatacgtg gcgctataga
     1141 aagacgcggc gaggcatttc tgcgacgtat ttacacgcct ggagaaattg cgtattgcga
     1201 gcggtttaaa aataaatttg agcgctatgc cgggcggttc gcggtgaagg aagctgccat
     1261 gaaggcgtta gggaccggct ggtcgcatgg agtgcgctgg gtagatttgg aagtgatccg
     1321 cgaaaaaagt gggcggccga cattagtgat cagaggcgaa gcgggaaaga tcgcggcgcg
     1381 cttaggcgtg aagaatattg tggtcagcat cacgcatacg gaagagcagg cgctggccca
     1441 agtaattttt gaaggctgag gaatttttgt gttaagcaaa tgcgcgaaag cttagctcgg
     1501 cgctaatccg ttccacaggc tctccgatta aatcgctctt ctcgacagac tggacgcgaa
     1561 cattcatcca ttgattgggg ggccaatggc cgcgcaatcg tacacgcaaa taattgttgg
     1621 agagcgcagg agtatagtca gactcttggt gcggcagtgg atccaccgat gccgagtcat
     1681 gccgtgactc tgccgaacgc agcgtaagca cgcgcaattg acggccgatc tgatctttgc
     1741 gaaatgcgtc ggactttctt tgagccagag cgcgtagttc ccgggcgcga tttcttatga
     1801 cacgcgctgg aacttcatgg ccaagcgttg cagcctgcgt accaggccgc ttggagaacg
     1861 agaatacgtg caggtaagtg agcggcaagc tttcgatgaa tgccagcgta gcggcgtggt
     1921 ccgcgtcgga ctcatggggg aatccggcga tcacatcggc tccaatggct gcgtgaggta
     1981 aaatttcgcg gattaaatta gcgcggcggg catagtgttc cgcgcgatac cagcgatgca
     2041 tggcggcgag gatgcgatcc gctccggact gcaagggcat atgaaagtgc ggcgccatgc
     2101 ggtcatttgc ggcaaaaaat tcgatgagat cagtggtcac gtccatgggc tcgatggaac
     2161 taacgcgcaa acgttcgagt ggcgtttcgt tcagaatacg gcgcagcaaa tcgtgaaaag
     2221 agaggcgagg gttgagatct cgtccatagg ttccgatatt tattccgctg agaacgattt
     2281 ctttcacccc tgaatcataa agttggttga cctgctgcag cactgtttcg agaggcaggc
     2341 tgcggctttt tccgcggact tgcgggatca cgcagtacgc gcagtgagag tcgcagccat
     2401 cctgaatttt gagtgtggga cgggtgt
//
LOCUS       DATCBB010000096         1760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_2877, whole genome shotgun sequence.
ACCESSION   DATCBB010000096 DATCBB010000000
VERSION     DATCBB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VII37_01125"
     CDS             complement(<1..472)
                     /locus_tag="VII37_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEY5068347.1"
                     /translation="MVLQKARVAFQGEAGAFSEAAAIQLLGDSITTVPRATFDATFAA
                     IKDGAADVLLVPVENSLAGSVVRVFDLLLESSLQIVAETIMPIEHHLIGCPGASLADI
                     RAVASHPMALAQCERLFNDHPSWKRVPAEDTAGSVREVLARQDKSLAAIAGQRAA"
     gene            602..1150
                     /locus_tag="VII37_01130"
     CDS             602..1150
                     /locus_tag="VII37_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY5068348.1"
                     /translation="MSYVEKNLVPGETLLYQTRHHWIVLIGPLLIALLVGLPGLFLIF
                     EVAAKRPEIYDYVTRMPGGAETLIAAGGVMVIIGLGILAWGMTKRNATEMAVTNRRVL
                     IKTGLGNRRTLDLMLSRVESIAVHETMWGRTLGYGDVVVHGTGGTPESFVLISHPQEF
                     RRQVQQQIGAHEVGGINETKQA"
     gene            1131..1664
                     /locus_tag="VII37_01135"
     CDS             1131..1664
                     /locus_tag="VII37_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5068349.1"
                     /translation="MKPSRPEADEYAPFYARYIALVPGDDVMAALEAQRLHMMQVLSA
                     RSEREGNFRYAPGKWRVKETIGHMSDTERVFTYRAMRIARGDQTPLPGFEQDDYVKNG
                     GFGERRLSDLAEEFAAVRSGSIALFRSLSEAAWVRRGTASGKEVSVRALAFMVAGHEL
                     HHQRILEERYFAAIPRA"
BASE COUNT          390 a          470 c          544 g          356 t
ORIGIN      
        1 tggcagcacg ttgcccggca atggcggcaa gagatttgtc ctggcgtgct agaacttcac
       61 gtacgctgcc cgcggtatct tccgccggca cccgtttcca cgaggggtgg tcgttaaata
      121 atcgctcaca ctgtgcaagc gccatgggat gagaagctac cgcgcgaatg tccgccaacg
      181 aagcgcctgg gcaaccgata agatgatgtt cgatgggcat aattgtttca gctacgatct
      241 gcaacgaact ttcgagaagt aaatcgaaga cgcgaactac cgagcccgcc agggaatttt
      301 cgacgggaac cagtagtacg tcagcagccc catcttttat cgccgcaaac gtcgcatcaa
      361 aagtcgcccg cggaactgtg gtgatggagt ccccgagaag ttgaatggct gcggcctcgc
      421 taaaggcacc cgcctcgccc tgaaatgcta cgcgcgcttt ctggagtacc atgtccctgc
      481 cgtcccaagt gtcaaaattg cgaagccgct agggtgcgct tcgctttgat gcgccttaga
      541 gtatcaagta tgatttcgcg cagcgcagaa aaacgacatg gcgctgctgg gcgaggacta
      601 gatgagctac gtagaaaaaa acttggtgcc tggcgagacg ttgctatatc aaacgcgtca
      661 tcattggatc gtgctaattg gaccgctgtt gattgccttg ctggtgggct tgcctggatt
      721 attcctgatc tttgaagtcg ccgcaaaacg cccggaaatc tatgactacg ttacgcgcat
      781 gccgggcggt gccgagaccc tgatcgccgc cggaggcgtg atggtgatta tcgggctggg
      841 gattctggct tgggggatga ccaagcgcaa cgccacggaa atggcggtga ccaaccgccg
      901 cgtactgata aaaactggat tgggcaaccg caggacgctg gacttaatgt tgtcgcgagt
      961 ggaaagtatc gcggtccatg aaaccatgtg ggggcgcacg ctgggttatg gcgatgtagt
     1021 ggtgcacggc accggtggaa ccccggaatc gtttgtgctc atttcgcatc cgcaggaatt
     1081 ccggcgccaa gtgcagcaac aaatcggagc gcatgaggtt ggtgggataa atgaaaccaa
     1141 gcaggcctga agcagacgag tacgcaccgt tttacgcacg ttacattgcg ctggtacccg
     1201 gcgacgatgt gatggccgca ctggaagcgc agcgcctgca tatgatgcag gtgctatcgg
     1261 cacgcagcga acgcgaaggt aacttcaggt atgcgcccgg caaatggagg gtcaaggaaa
     1321 ccatcggaca tatgtcggac accgagcggg tttttaccta ccgcgccatg cgtattgctc
     1381 gcggcgatca aaccccactg ccagggttcg aacaggacga ttacgtcaag aacggcggct
     1441 ttggcgaacg acgcctttcc gatctcgcgg aagaattcgc ggcggtgcgc agcggcagca
     1501 tcgcgctatt ccgatcgcta agtgaggcgg cctgggtgcg tcgcggcacg gccagcggaa
     1561 aagaagtcag cgtgcgggcg ttggcattca tggtcgccgg gcacgaactg catcaccagc
     1621 gcatattgga agaacgctat ttcgcggcga ttccgcgcgc atagttgatc tgcaccgttc
     1681 aattcacggt tgctcgccgg caccagcggt gaggtgggca tcactcctgg cgagcgtgct
     1741 ggtgtgtgca acggctcgta
//
LOCUS       DATCBB010000097         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_457316, whole genome shotgun sequence.
ACCESSION   DATCBB010000097 DATCBB010000000
VERSION     DATCBB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457316"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            979..2112
                     /locus_tag="VII37_01140"
     CDS             979..2112
                     /locus_tag="VII37_01140"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEY5068350.1"
                     /translation="MAHRFTIGVEEEFQIIDPATCELRSHVMQLVSEASPSIVEQVKH
                     EMHQSIVETGTNICASVHELRIEMHRTRGELVAAAERAGLQVAAAGTHPFSSWIDQVI
                     SPGERYQNIIEELQQLARSLLIFGMHVHVAMPDKQTTIDMMNMVRYFLPHLLALSTSS
                     PFWMGRNTGLKSFRTTVFRRFPRTGVPEQFESWSAYENFVNLLIKLNCIDNGKKIWWD
                     VRPHPTFGTLEFRMCDVATKVEEAVAIAALTQAIVVKLHRLYKGNMSFRLYRRALIEE
                     NKWRAARYGIEGKLVDFGKETEVPMRELIPELLEFVDDVVDELGSRSAVEYVHTIMNE
                     GTSAERQLRVYQETGDLKAVVQHLVNETRGGVTQSSSSARAVN"
     gene            2222..3364
                     /locus_tag="VII37_01145"
     CDS             2222..3364
                     /locus_tag="VII37_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068351.1"
                     /translation="MKKVGLLCGREFSFPPAFIERVKTLGAKDGVTAEFVKLSGTRMD
                     EPAKYRVIVDRISHEVEYYRGYLKHAVLQGSYVINNPFWWTADDKYFNYCVAAKLGVA
                     IPKTVLLPQKGYPKDVDINAESLHNLEYPIDWDGLLDYVGRPAILKPFSGGGWKHVYK
                     VHTKEELWAAYDQTSPYCMTLQEFIDFTQYVRCFTFGKTDILPVHYDPKGRRYIVDHK
                     YLSAEIGERVVRDAQTINQALGYEMNTIEFAIQDGVPYAIDFLNPAPDFERDRITEFY
                     FEQVVDKMARLVIDRALGGEVSNSWPRWEEMVGINAGTQEGGVGGFTGAPKLKTQAAA
                     APSQSTPAQTAQKPSAPAAARTPSAAGQTVSPSTTTPASPSKSKTK"
     gene            3361..>3914
                     /locus_tag="VII37_01150"
     CDS             3361..>3914
                     /locus_tag="VII37_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068352.1"
                     /translation="MSAASPRPVDGSAAEYWHALLHPALKKDDALAENFLKELRSGKL
                     TFGDRVHCPFLRPFFLSPQDEDRVRPVAETIAELGERVALAALADEKLFAQFHLRPEE
                     ARLARLPGGNGRVSTASRLDAFLLPESLKFAEYNGESPAGAGYSETLGEIFSELPLMR
                     QFSKRYEVHGYPLSAKLLDALVLS"
BASE COUNT          921 a          970 c         1162 g          861 t
ORIGIN      
        1 cgcttcaagc gcaaagtgca gcaggaagac atcatcaagg aaataaagaa gcacagcttc
       61 tacatgaaac ccggcgagaa gcgccggacc aaagaagcgc tttcgcggaa gcgactgcgt
      121 aagaagcagc gccgggaagc ggattaactt ccgtttcgcg gctcggctcg gcgcggcact
      181 tccgagaatc aggccccagt caggtaagtg agttgggttt ctctttcggc ttcacgcaag
      241 caggcccttg ggaaccgcgc ccggcggccc cggatatcgg cataccctcg cagtgggatg
      301 ttccacgaag tcaggcctct cggtgcaagt tgacccatcc ccttcgcctc cctggggtta
      361 aggggagcgc ctcatggaat atcacgatcg ggtcctgaag tgttcggagt gcagcgccga
      421 gtttgtattt actgccggcg aacaaatgtt tttcgccgat aagggtttca agaacgaacc
      481 gaagcgatgt aaagggtgca aagcaagacg cgcggagggt gctggtccgg gcgttgcggg
      541 tggattgcaa atgcgctcgg aaacaaaaac caattgttcg cagtgcggca aggagactac
      601 tgttcctttc cgcccgaccc agggacgccc ggtattttgc cgggaatgct tccagcagcg
      661 caggtcaatg ggggcggctt agttcgcttt agcggctcgc aataagtgca tcgggggcag
      721 cactgcacat ctgtccccaa ttttcctcgc aagtcctcca gacgcaaacc cggaatgcac
      781 tgctgtggtg ctacgatttg cactatttta cgccaatctt tttgacagtt gctccctgca
      841 tccagccaag gcttagaatt ctcgcaacat agcaattgaa atttacctgg gcagggttcc
      901 gatataaatt aagggacgag gggcgtatca tacgcacctt aagtcgcacg aatcgtcagg
      961 gtcaaataga gggccatcat ggctcaccgt ttcaccatcg gtgtcgaaga agaatttcag
     1021 attatcgatc cggccacgtg tgagctgcgt tcgcacgtga tgcagctggt ttccgaggct
     1081 tctccgagca tcgtggagca ggtgaagcat gaaatgcacc agtcgatcgt ggagacgggc
     1141 acaaatattt gcgccagcgt gcacgagctg cggatcgaga tgcaccgcac gcgaggcgaa
     1201 ctggtggccg cggcggaacg cgcgggacta caggtggcgg cggcggggac gcacccattt
     1261 tccagttgga tcgatcaggt aatttctccc ggggaacgct atcaaaatat aattgaagag
     1321 ctgcagcaat tggcgcgttc gctgctgatt tttgggatgc atgtgcacgt ggccatgccc
     1381 gataaacaaa ccaccatcga catgatgaac atggtgcgtt acttcctgcc gcatcttctg
     1441 gcgttgtcga ccagctcgcc attctggatg ggacggaata ccgggctcaa atcgtttcgc
     1501 accaccgttt tccgcagatt tccgcgaacc ggggtgccgg aacaattcga atcgtggagc
     1561 gcgtacgaaa attttgtgaa tttgctgata aagctgaatt gcatcgataa cggcaaaaag
     1621 atctggtggg atgtgcggcc gcacccgaca ttcggcacgt tggaatttcg catgtgcgac
     1681 gtcgccacaa aagtggaaga ggcggtggcc attgcagcgc ttacgcaagc tatcgtcgta
     1741 aaactccatc gactgtataa aggcaacatg agcttccggc tgtaccggcg tgcgctaatc
     1801 gaagaaaata aatggcgagc cgcgcgctat gggattgaag ggaagctggt ggacttcggc
     1861 aaggaaacgg aagtgcccat gcgcgagttg attcccgagc tgctggagtt tgtggatgat
     1921 gtggttgatg agctaggcag ccgcagcgct gtggagtacg tgcatacgat catgaacgaa
     1981 gggaccagcg ccgagcggca gttgcgggta tatcaggaga cgggcgacct taaagcagtg
     2041 gtgcagcatc tggtcaatga aacccgcggc ggagtgaccc agtcgtcgtc atcggcgcgg
     2101 gctgtaaatt aatttgcggg tttgttatcg gctttagttt ccgattttct tctgatctta
     2161 aaccactagc ccgttacgaa tagttagaat gatgctgagt tttttttgag gagcaagagc
     2221 aatgaagaag gtggggctat tatgcgggcg ggaattcagc tttcccccgg cattcattga
     2281 acgggtaaag actttaggtg caaaagatgg cgtcactgct gagtttgtaa aactgagcgg
     2341 gacgcgcatg gatgagcctg cgaaataccg ggtaatcgtg gaccgcattt cgcacgaagt
     2401 ggaatattat cgcggctatt tgaaacacgc ggttctgcaa ggaagttacg tcatcaataa
     2461 tccgttctgg tggacggcgg acgacaagta tttcaattat tgcgtggcgg caaaactcgg
     2521 cgtggccatt cccaagacgg tgttattgcc gcaaaaaggc tatccgaaag atgtggatat
     2581 taatgcggaa tccttgcaca acctggagta tccgatcgac tgggacggtt tgcttgatta
     2641 cgttgggcga ccggcaattc tgaaaccttt ttcggggggc ggctggaaac acgtatataa
     2701 agttcacacc aaggaagaat tgtgggcagc ctacgatcag acttcgccgt attgcatgac
     2761 gctgcaggaa tttattgatt tcacgcaata cgtgcgctgc ttcacattcg gcaaaacgga
     2821 tattttgccg gtgcactacg accccaaggg ccgccggtac atcgtagatc acaaatatct
     2881 ttccgcagag attggcgagc gggtggttcg agatgcgcaa acaattaatc aggcgctggg
     2941 atatgagatg aacaccatcg aatttgccat tcaggatggc gtaccgtacg ccatcgattt
     3001 tctgaatcca gcgccggatt tcgagcgcga ccgtattacc gaattttatt ttgagcaagt
     3061 ggtggacaag atggcgcgat tggtgatcga tcgcgcgctt ggtggagagg tttccaattc
     3121 gtggccgcgt tgggaagaga tggtcggtat taatgcgggg actcaggaag gcggcgttgg
     3181 tgggttcacg ggcgcaccga aactgaaaac gcaggctgcc gcggcgccgt cgcagtcaac
     3241 gccagcgcaa acagcgcaaa agccctcggc gcctgcagca gcgcgaacac cgtctgcagc
     3301 agggcagact gtttccccat cgacgacgac gcctgccagc ccaagcaaat cgaaaacaaa
     3361 atgagtgcgg cttcgccaag acccgtggat ggcagtgcag cggaatactg gcacgctttg
     3421 ctacatcccg cgctaaagaa agatgacgcc ttggcggaga atttcctgaa agaactgcgc
     3481 tccggcaaac tgaccttcgg cgatcgtgtg cactgccctt ttcttcgacc cttctttctt
     3541 tctcctcaag atgaggatcg cgtgcgcccg gtggccgaga ccattgcgga acttggagag
     3601 cgggtagccc ttgcggcgtt ggcggacgaa aaattgtttg cgcaatttca tttgcgccca
     3661 gaggaagcac ggctggcgcg tttgcctggc ggaaacggca gggtcagcac ggcctcacgc
     3721 ctggatgcgt ttttgctgcc cgaatcgctg aaattcgcgg aatacaacgg cgaatcaccg
     3781 gcgggtgcag gttattcaga gacgctgggc gagattttca gcgaacttcc gttgatgagg
     3841 caattttcaa agcgctatga agtgcacggt tatcccctga gcgcgaaact gttggacgcg
     3901 ttggtactga gtta
//
LOCUS       DATCBB010000098         1921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_67302, whole genome shotgun sequence.
ACCESSION   DATCBB010000098 DATCBB010000000
VERSION     DATCBB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67302"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VII37_01155"
     CDS             <1..1337
                     /locus_tag="VII37_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY5068353.1"
                     /translation="PDEVRPVLESALDALTYLHAQGLAHTRIKPGNILAAGDQLKLSS
                     DTLSEIGTAPTATPHAGIYAAPESATASISAAADVWSLGVTLVEALTQRAPVVRGQME
                     VELPPETIPSPYLEIVRRSLQVEPSRRASLKEISALLNPTSTTPYREPILHSDIHTPV
                     AQHPEIPAPTPEHHDRPKALPVAETVAAVEPPKKSLASQQVSLLPHTLLPASPVDDTR
                     TKPARRKSRVAIPLVAVVLVLAAILLAPRVLNRFSQLKPQAATVEPSIAPAPSSLTPP
                     KTEAPVLPPAMQTKAEKRAAEKQAAREKTAAERETVSSSEAVNPVSSRSVDAAQPRPV
                     AKSAKVAPTNAARGAVLYQVVPDISQRARDTIQGTVRVGAKVHVDDTGKVVGAELDNF
                     SSKFFGDQAVQVARRWTFTPPQVDGRNAPSEWVLRFEFTQQATKVFPTQTNP"
     gene            complement(1394..>1921)
                     /locus_tag="VII37_01160"
     CDS             complement(1394..>1921)
                     /locus_tag="VII37_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5068354.1"
                     /translation="GALPGDLVAVQGIGGLGHLGVQFANKFGYRTVAVGRGAENAGLA
                     KKLGAADYIDSKATNAAQALKKMGGARVILATAPSSKAMSELIDGLGPNGTLVVVGAT
                     MDPIEVTPIQLIMGRRGIQGWASGIATDSEDTLRFAELTGVRPMIEKYPLAKAAEGYA
                     RMMSGKAEFRAVLTM"
BASE COUNT          411 a          589 c          542 g          379 t
ORIGIN      
        1 agccggatga agtacggccg gtgctggaat cagcacttga tgcgctgacg tatctgcacg
       61 cacaagggct ggcacatacg cgcattaagc cgggaaatat tcttgcggca ggcgatcaac
      121 tgaagttatc gagcgatacg ctgagcgaaa tcgggaccgc gcccacggcg acgccacacg
      181 ctggcattta cgcggcaccg gaatccgcga ccgcgtcaat ctccgcggcg gcggacgtgt
      241 ggtcgctggg agtgacatta gtggaagcgt taacgcaacg cgcgccggta gtccgcggcc
      301 aaatggaagt tgagctgcca cccgagacga taccatcgcc ctatctggaa attgtgcggc
      361 gtagcttgca ggttgaacct tcgcggcggg ctagcttgaa ggaaatttcg gccctgctga
      421 atccaacttc gacaactcct tatcgtgagc cgattctaca tagcgacatt cacactcctg
      481 tggcgcaaca tcccgagatt ccggccccca caccggaaca tcacgacagg ccaaaagcgc
      541 taccggtcgc cgagacggtt gctgcggtcg aacccccgaa aaaaagtctg gcgtcgcagc
      601 aagtttcttt gctcccacac acattacttc ccgcttcgcc ggttgacgat acaaggacga
      661 agccagcgcg acgcaaatct cgagtagcta ttccgctggt ggcggtggtg ctggtgctgg
      721 ccgcgatact gctggcgccg cgcgtactca atcgcttttc tcagttgaag ccgcaagccg
      781 caacggtgga gccgtcaatt gctcccgcgc catcgtcttt aacgcctccc aagactgagg
      841 cgccagtttt gcctccggct atgcaaacca aggccgaaaa acgcgcggca gaaaaacaag
      901 ctgcgcggga gaagactgcc gctgagcgtg aaacagtttc gtcaagtgag gctgtaaacc
      961 cggtctcaag ccgctccgtt gacgcagccc aacccagacc ggtggccaag tctgccaagg
     1021 tagcgccaac aaatgcagct cgcggggcag tgctgtacca agtggtgccg gacatctccc
     1081 agcgcgcgcg cgacaccata caaggaaccg ttcgcgttgg cgctaaagtt catgtcgatg
     1141 acaccggtaa ggtggttggc gcggaattag acaatttttc cagcaagttt tttggggatc
     1201 aagcggtgca ggtagcgcgc cgctggactt tcacgccgcc tcaagtggat gggcgcaacg
     1261 cgccgagcga atgggtgctg cgcttcgagt tcacacagca ggccaccaag gttttcccga
     1321 cgcaaaccaa cccgtaacga tattgccagt aagatcgggc gatttcgcag cggcctcgct
     1381 aaacttccgt tttttacatg gtcaacactg cgcgaaattc cgctttacca ctcatcattc
     1441 gcgcgtagcc ctccgccgcc ttcgcgagcg gatatttctc aatcatcgga cgcacgccgg
     1501 ttaactctgc gaaacgcagg gtgtcttccg aatcggtagc tatgccggaa gcccatccct
     1561 gaatgccccg gcgtcccatg attaattgaa tcggtgtcac ttcaatcggg tccatcgtag
     1621 cgcccacaac taccagcgta ccgttgggac ctaacccgtc gataagttcc gacatcgctt
     1681 tggagctggg agcggttgcg agaatcaccc ttgcgccacc catttttttc agcgcctgcg
     1741 cggcattggt ggccttgcta tcgatgtagt ccgcggcgcc gagttttttg gcgagccccg
     1801 cgttttccgc gccgcgacca acggccacgg tgcgatatcc aaatttgttg gcgaactgta
     1861 ccccaagatg gccgagcccg ccgattccct gcaccgcgac gagatcgccc gggagggcac
     1921 c
//
LOCUS       DATCBB010000099         1483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_392447, whole genome shotgun sequence.
ACCESSION   DATCBB010000099 DATCBB010000000
VERSION     DATCBB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392447"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..>1483
                     /locus_tag="VII37_01165"
     CDS             267..>1483
                     /locus_tag="VII37_01165"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY5068355.1"
                     /translation="MSKEAKLELDGKVFTLPTVTGSEGEKAVDISALRAETGYITLDD
                     GYGNTGSCTSKITFIDGDKGILRYRGIAIEALAEKSTFIETAYLLINGDLPNRAQLKQ
                     FSDLLTEYQFIHEDMKYHFEGFPTGAHPMAILSAMINAASCFDEDLMRWQWGKTKGFE
                     VYVAKLISQVRTIAAYSYRKSHGLPLIYPKKAYKYTANFLHMMFSSPYEDFEIKPEVV
                     KALDLIFLLHADHEQNCSTSTVRMVASSQANLFASASAGVCALWGPLHGGANQAVIEM
                     LEEIHRSGDDGSRFIADAKVKGSGKKLMGFGHRVYKNYDPRAKIIKKACDEVLSQLKI
                     NDPLLDIAKHLEEAALKDAYFVERKLYPNVDFYSGIIMRAIGIPVEMFPVIFAIGRMP
                     GWIANYKEIMEDS"
BASE COUNT          352 a          375 c          432 g          324 t
ORIGIN      
        1 gcggtccgta gtggctttca ttcttgcccc agttgccaaa ggccatgagg gccaccggca
       61 gggtgagcag caccagcagg agcgcgtaaa gcaagacggt gaggacagcc cagcgtttca
      121 tgacatgttg atttggccac gtaatggaat cctgcgccag cgaaatccgg gagaactttt
      181 cgcaaaagcc ccctttcgcg tcttgctaat tttttatttc atcagcagat ttcttaaaat
      241 tcgaaataat caacgtaaca agggatatga gcaaagaggc caaactggaa ctggacggaa
      301 aagttttcac gctgccgacg gtcaccggga gcgagggtga aaaggcggtg gacatttccg
      361 cgctgcgcgc ggaaaccggc tacatcacgc tcgatgacgg ctatggcaac accggctcct
      421 gcacgagcaa gattaccttc attgacggcg acaaggggat tttgcgctat cgtggcatcg
      481 cgatcgaggc gctggcggaa aaatccacct tcatcgagac ggcctacctg ctcatcaacg
      541 gcgacctgcc gaaccgcgcg cagctcaaac aattttcgga tctgctgacg gagtatcagt
      601 tcatccacga ggacatgaaa tatcatttcg agggttttcc caccggcgcg cacccgatgg
      661 cgatcctctc ggcgatgatc aacgcggcca gttgctttga cgaggatctg atgcggtggc
      721 agtggggcaa gacgaagggc tttgaggtgt atgtcgccaa attgatttcg caggtgcgga
      781 ccatcgcggc gtattcgtat cgcaaatcgc acgggctgcc attgatttat ccgaagaagg
      841 cctacaaata tacggcgaac tttctgcaca tgatgttctc gtcgccgtat gaggattttg
      901 aaatcaagcc cgaggtggtg aaagcgctgg acttgatatt tttgttacat gcggatcacg
      961 agcagaattg ttccacttcg acggtgcgca tggtggcgtc gagccaggcg aatctatttg
     1021 ccagcgcatc cgccggggtc tgcgcgttgt gggggccgct gcatggcggc gcgaaccagg
     1081 cggtgattga aatgctggag gagatccacc gttccggcga cgacggctcg cggttcatcg
     1141 cggatgcgaa ggtgaagggc agcgggaaaa aactcatggg tttcggccat cgcgtctata
     1201 aaaattacga cccgcgcgcc aaaatcatca agaaggcctg cgacgaagtt ttgtcccagt
     1261 tgaaaatcaa tgacccgttg ctggacatcg ccaagcatct ggaggaggcc gcgttgaaag
     1321 atgcctattt tgttgagcgc aaactctatc cgaacgtgga tttttacagc ggcatcatca
     1381 tgcgcgccat tggcatcccg gtggaaatgt ttccggtgat cttcgcgatt ggccggatgc
     1441 ccggctggat tgcgaattac aaggaaatca tggaagattc caa
//
LOCUS       DATCBB010000100         1125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_229928, whole genome shotgun sequence.
ACCESSION   DATCBB010000100 DATCBB010000000
VERSION     DATCBB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229928"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VII37_01170"
     CDS             <1..355
                     /locus_tag="VII37_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY5068356.1"
                     /translation="AANSNADSANRELGSDDAQLGIVQRVMPQVSPGARRTIQGKIKV
                     RVRVGADASGNVSSATFESAGSSKYFSRISMEAAREWKFVPADAAETAGRREWKIQFA
                     FTRARTEASAVRVKR"
     gene            complement(475..>1125)
                     /locus_tag="VII37_01175"
     CDS             complement(475..>1125)
                     /locus_tag="VII37_01175"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY5068357.1"
                     /translation="PVYTRVLHWSVAVFFFLALFTGFGIYLPWLFRWFTPIFGGGQLS
                     RILHPYFGVAFVFFFALQALNWLQPMLWTNADSRWMRNIKKVVTGEEKLEPPDTGFFN
                     AGQKLQFWEIVGGCIVYLITGIVLWAGAKTFGRITVAVSYVLHDISAIIMLGGIFVHI
                     YLSTIGEPGTFQSMIRGAVSEAWAWTFHPAWYKQITGRDQKQAYEEALRQSNSNKP"
BASE COUNT          252 a          268 c          367 g          238 t
ORIGIN      
        1 ggcagctaac tccaatgctg attctgctaa tcgagagttg ggcagcgacg atgcacagtt
       61 gggaatagtg cagcgcgtga tgccgcaagt ttcgccgggc gcgcggcgca cgattcaagg
      121 gaagatcaag gttcgagtca gggtcggcgc ggacgcatcc ggcaatgtgt cgagcgcgac
      181 gttcgaatcg gccggttcga gcaagtattt ttcgcgcatt tcgatggagg cggcacggga
      241 gtggaagttc gttccggcgg acgcggcgga gacagcgggg cgtcgggaat ggaagattca
      301 gttcgccttc acgcgggcac ggacggaagc ttcggcggtg cgggtcaagc gctagggctg
      361 ttgagggagt aggggtcctt cgactccgca tgatcttcac ttcgtgaaga tcatgctgcg
      421 ctcaggatga caatttgttt gggttgcaaa ctggttggat tgcaatgtgt tggattaagg
      481 tttgttgcta tttgactgac gcagcgcctc ttcgtaggcc tgtttttgat cgcgtccggt
      541 gatctgctta taccatgcgg gatggaatgt ccacgcccat gcttcgctta cggcgccgcg
      601 aatcatggac tggaaagtgc cgggctcgcc gatggtgctg agatatatgt gcacgaagat
      661 tccgccgagc atgatgatgg cagagatgtc gtgcagaacg tagcttacgg ctactgtgat
      721 gcggccaaaa gtcttcgcac ccgcccataa aacaatgccg gtaatcaagt agacgatgca
      781 gccgcctacg atctcccaga actggagttt ttgtccggcg ttgaagaagc cggtgtctgg
      841 cggctcaagc ttttcctcac cggttacgac tttctttata ttgcgcatcc agcgggaatc
      901 ggcgttggtc cagagcatgg gctggagcca gttcagggct tgcaaagcga aaaagaaaac
      961 gaaggctacg ccgaaataag ggtgcaggat gcggctcaat tgtccgccgc cgaagatggg
     1021 cgtgaaccag cggaaaagcc aaggcaggta aatgccgaag ccggtgaaga gtgccagaaa
     1081 gaaaaacacc gcaactgacc agtggagcac acgcgtgtag acggg
//
LOCUS       DATCBB010000101         1746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_67362, whole genome shotgun sequence.
ACCESSION   DATCBB010000101 DATCBB010000000
VERSION     DATCBB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67362"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VII37_01180"
     CDS             <1..758
                     /locus_tag="VII37_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068358.1"
                     /translation="NSAGQKAGYFYVWMAAVCALIAFGAFAPTYWLQLSAGTFVGPPL
                     LHIHGALFFAWTLLLLSQTALAANGRLESHGAWGLGGITLATAMVVVGIAASIHTLTT
                     GLAAGYGDASRAFFILPLSAIVLFAGFFVAAVSNIRRPEVHKRFMLLATISLLQAAMG
                     RIFFILATGGGPGMRPGLGPPPPLFIGLVPSLILELMIVAGIVYDWRTRGQPHPVWLM
                     GAVVMTAVILLRGPFAGTRAWLAFADALAHIAS"
     gene            complement(1377..>1746)
                     /locus_tag="VII37_01185"
     CDS             complement(1377..>1746)
                     /locus_tag="VII37_01185"
                     /inference="COORDINATES: protein motif:HMM:NF045160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068359.1"
                     /translation="ANVHAALSFLVEWPAHERAAQLVLSRNKEIDGDRYELLDPAARV
                     IEGKYPLAAVLLRRALIEVTLRKGRATRYKHAARHVREIDSLNSQIRDYKNFETHDQF
                     MERLRKTHLRKTGFWPLLRD"
BASE COUNT          334 a          486 c          499 g          427 t
ORIGIN      
        1 tcaattcggc aggacagaag gcgggctatt tctacgtctg gatggcggcg gtttgcgctc
       61 tgattgcttt tggagcattt gcgccgacct actggctgca gttgtccgcc ggaacctttg
      121 tgggcccacc actccttcac attcacggcg cgctgttctt tgcctggacg ctgctcctgc
      181 tttcgcagac agcgctcgcc gccaacggtc ggttggaaag ccacggtgct tggggtttag
      241 gaggtatcac gctcgccacc gctatggtcg tagtcggaat agcggcgtcg atccataccc
      301 taacgacggg actggcggcc ggttacggag atgcctcgcg cgccttcttt attttgccgc
      361 tcagtgcgat cgttcttttt gccggtttct ttgtcgccgc ggttagcaat atccgacgcc
      421 cggaggtgca caagcgtttc atgctgcttg caaccatctc tttgcttcaa gcggcaatgg
      481 gacgcatctt ttttatattg gccaccggtg gcgggccggg catgcggcca ggactgggcc
      541 caccaccacc tttatttata ggacttgtcc caagtctaat ccttgagctt atgatcgtcg
      601 cgggaattgt atatgattgg cgcacgcgcg gtcagccgca tccggtttgg ttgatgggcg
      661 cggtcgtgat gaccgccgtg atcctgctga gggggccgtt cgctggcacg cgggcatggc
      721 tggcttttgc cgatgccttg gcgcacattg ccagctgagg agaaaagcgt cgccttgaga
      781 tttagggagc cgaatgatca gttcatacgc gcgttcccat gcgttctcgc tgtcacatga
      841 ccgttgccga atgtagttcc tcacggtgtc gtagctgccg gcaaaaccct cctggcgtcg
      901 taggtgctca aaaatatccc gcatggttag atcggccttc ggcgggaata aaatgatgtc
      961 gtgcaaaagg taatcgatcg tgtggatgta tggacccaat ttcgggtacc ggcgcggtct
     1021 agggtggtag ccaggaggaa actcgcgggc cagcatcctg ttgatcgttt gacggctgat
     1081 tcccgtctcg cgagagacct gtttttttgg tgttcctttc accacgatac ggcgacgaat
     1141 gtgccgccac tgccggacat cgcaatacat gaaatgctcc tccacgcgga tcgatcagac
     1201 gtccacaagg aagaattgct ttcgaaaaaa gagtattgaa tctagctagt tgcgcatgac
     1261 cgggcggcct tcggtatcga gactttggct aggacgaccg ctttgggtcg aaatgcgaaa
     1321 tcctcaatac gagcaaatag gtcagacccc ccataaccgg tcctcccgtg cgagtttcaa
     1381 tcgcgcagca gcggccaaaa tcctgtcttc cgcagatgcg tctttcgtag cctctccatg
     1441 aattggtcgt gcgtctcaaa attcttgtag tccctaattt gcgaattcaa actatcaatc
     1501 tcacggacat gccgcgcagc gtgcttgtag cgtgtggcac ggcccttccg cagcgtgacc
     1561 tcgatgagcg cgcgccggag caaaaccgca gccaatgggt attttccttc aataacccta
     1621 gccgctggat caagcagttc gtagcggtcg ccgtcgattt ccttattccg tgacagcacc
     1681 agttgagcgg cacgctcgtg ggccggccat tccactagga aggacagcgc ggcatgaacg
     1741 ttggca
//
LOCUS       DATCBB010000102         3269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_197726, whole genome shotgun sequence.
ACCESSION   DATCBB010000102 DATCBB010000000
VERSION     DATCBB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197726"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VII37_01190"
     CDS             <1..512
                     /locus_tag="VII37_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5068360.1"
                     /translation="VWNNICYGLTGVAEERVLAAARAALAHDFIQELPLGYNTLVGER
                     GTRLSGGQRQRIAIARAILKDSPILILDEATSELDAESEMFVQKALANLMVGRTTFVI
                     AHRLATIRRADKIMVLEDGQIRESGTHTELMAKSGTYARLHDIQFADDDMLAPARLSP
                     ETSVKSQLL"
     gene            554..1444
                     /locus_tag="VII37_01195"
     CDS             554..1444
                     /locus_tag="VII37_01195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY5068361.1"
                     /translation="MTGYAQARADTTDLALRVSIKSVNHRFLDLKMRLADGLELYEPR
                     LRQMVRERIHRGHVELQVSAEPRTAATIQVNQELVRAYMRAAEALRQETRAAADVDVV
                     ALLRLPGVISGLGIATPQTEESQEKLGRALEDCLGEALSKLDDMRRAEGSHLIEELNK
                     RLTKIEGEADQVRVLAETLRPAFARRLEARLAELLSGVSLEPARLAQEAALLAERSDI
                     TEELERLRSHVAQFANLLRGAGEMGKKLDFLLQEMHREANTLLSKTPGVDAEALAITG
                     LALEIKADIEKLREQVQNIE"
     gene            1441..2076
                     /gene="gmk"
                     /locus_tag="VII37_01200"
     CDS             1441..2076
                     /gene="gmk"
                     /locus_tag="VII37_01200"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY5068362.1"
                     /translation="MMAEAKIAPLVIIVSGPSGSGKSTLVQKVLEMPGTMPSISCTTR
                     GRRATESSGKCYDFVTEAEFEAMIARGEFLEYARVFGRHSYGSPKKWLEESQRLGLDL
                     VLEIDVQGASQVKRQLPESVAIFILPPSRQELERRLRSRGQNSDEDIARRLQKARDEI
                     AAFGKYYDYCVVNEDVERAGREVQAIVTALRCTKARRQDLVEKLLASFGGE"
     gene            2073..2309
                     /gene="rpoZ"
                     /locus_tag="VII37_01205"
     CDS             2073..2309
                     /gene="rpoZ"
                     /locus_tag="VII37_01205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY5068363.1"
                     /translation="MTEMTVLTKAPESQFAYVVLAARRARQIMAGAPPLVENPRTQKA
                     TRIAVEELDHSLLEYDFVEPDSIDEDKDGNKRRK"
     gene            2331..>3269
                     /gene="coaBC"
                     /locus_tag="VII37_01210"
     CDS             2331..>3269
                     /gene="coaBC"
                     /locus_tag="VII37_01210"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY5068364.1"
                     /translation="MRITMGVTGGVAAYKAAELVRLLQQDGFTVEVVMTRGAREFITP
                     LTFAALTGQKVITDLFGNSQDEANLESAIEHIAVAQRTDLLLVAPATADIIAKFARGI
                     ADDFLTTLYLASTAPVVVAPAMNVNMWNHPATQENIAKLRERGVNVVQPDEGYLACGM
                     VGAGRLAGHQEILAAVRATLHSNKDLAGETLLVTAGPTCEDLDPVRYLTNRSSGKMGY
                     AVAEAAARRGAKVILVSGPTALETPLGVRRVDVRTAEQMHEAVTKEFAACSIGIFAAA
                     VADYRPAEKHAEKIKKNDGNLTIHLEPTVDILQDVAN"
BASE COUNT          761 a          765 c         1092 g          651 t
ORIGIN      
        1 cggtgtggaa caacatctgt tatgggctga ctggagttgc tgaagagcgg gtgctggcgg
       61 ccgcgcgggc ggcactcgct catgatttca tccaggagtt gccgctgggt tacaacacgc
      121 tggtgggcga acgcggaacc agattgagcg gcggacaaag gcagcgcatc gctatcgcac
      181 gagcgattct gaaggattcg ccgattttaa tcctcgatga agcgacgtcc gagttggatg
      241 ctgaatcgga aatgttcgtg cagaaagcgc tggcaaactt gatggtgggc cgcacgacgt
      301 tcgtaattgc acatcgtttg gccaccattc ggcgcgccga caaaattatg gtgttggaag
      361 acggacagat tcgtgaaagc ggcacgcata cggagttgat ggccaaaagc ggaacgtacg
      421 cgaggctgca cgacattcaa ttcgcggatg acgacatgct ggcgccggca aggttgtctc
      481 ccgagacgag cgtcaaatca caacttctat gagccaacct cccgctgcga ccaggaaacc
      541 cgaatgcctt tcgatgactg gatacgcgca agcgcgcgcg gacaccaccg atctggcatt
      601 gcgcgtcagc atcaagagcg tcaatcaccg ttttctcgat ttgaaaatga ggctggcgga
      661 cggattggag ctttacgagc cgcggttacg gcagatggtg cgagaacgca tccatcgagg
      721 tcacgtagaa ctgcaagtaa gcgccgagcc ccgcaccgct gcgacgatac aagtgaatca
      781 ggaattggta agagcttata tgcgtgcggc ggaagcgttg cgacaggaaa ctcgcgcggc
      841 tgcggacgtg gatgtggtgg ctttactgcg tttgcctggc gtgatttcgg gattgggtat
      901 cgccacgccg caaacggaag agagtcagga aaaactcggg cgagcgctcg aggattgcct
      961 gggggaagca ctgtcgaagc tcgatgatat gcgccgcgcg gagggcagcc atcttatcga
     1021 ggaacttaat aaacggctca caaagatcga aggagaagcg gatcaggttc gcgtgctggc
     1081 ggagacgttg cgtcccgcgt ttgcgcggcg gcttgaggca cggcttgcag aattgttgag
     1141 cggagtttca ctggagccgg cgcgactcgc acaggaggcc gcgctgcttg cggagcgcag
     1201 cgacattacc gaagaactgg aaagattgcg gagtcacgtg gcgcaatttg caaacctgct
     1261 gcgcggcgcc ggtgagatgg ggaaaaaact cgattttctt ttgcaagaga tgcatcgcga
     1321 agcaaacacg ctattgtcga aaaccccggg agtggacgcg gaggcgctgg caattaccgg
     1381 gctggcgctg gagattaagg cggacataga aaaattgcga gaacaggttc agaacatcga
     1441 atgatggcgg aagcaaagat tgcgccgtta gtgatcatcg tgtccgggcc gtcgggttcc
     1501 ggaaaatcga cgttggtgca aaaagtattg gaaatgcctg ggacgatgcc ctcgatatca
     1561 tgtactacca gggggcgtcg cgcgaccgag tcgagtggga agtgctatga tttcgtgacg
     1621 gaagcggaat tcgaagccat gatcgcccgc ggcgagttcc tcgaatacgc gcgagtgttt
     1681 ggcaggcact cgtatggttc gccgaagaaa tggttggagg aatcgcaaag gttaggactc
     1741 gacctggtat tggagatcga tgtccaaggg gcttcgcagg tgaagcggca gttgccggag
     1801 tcggtggcga tttttatttt gccgccttcg cggcaagagt tggaaaggcg gctgcgctcg
     1861 cgaggacaga attcggacga ggatatcgcg cggcgattac aaaaagcgcg cgatgaaatc
     1921 gcggcgtttg gaaaatatta cgattactgc gtcgtgaatg aagatgtgga acgtgcagga
     1981 cgcgaagtgc aagcaattgt aacggcgctg cgttgcacca aagcgcggcg tcaggacctg
     2041 gtagaaaaac ttctggcatc gtttgggggg gaatgactga gatgacggta ctgacaaaag
     2101 ctccggagag ccagttcgcg tatgtggtgc tggcggctcg gcgcgcgagg cagatcatgg
     2161 ccggcgcacc accgctggtg gaaaatccaa gaacgcaaaa agctacgcga atcgcggtgg
     2221 aagaactcga tcatagtttg ctcgagtacg atttcgtgga gccggattcg atcgacgagg
     2281 ataaggacgg caacaagcgc cgtaagtagc gcggcgctgc cagggacgcc atgcgaatca
     2341 cgatgggtgt gaccggggga gtcgcggctt acaaggctgc ggaactggtg cgcctgttgc
     2401 aacaagatgg gtttaccgtg gaagtggtga tgacccgggg cgcgcgggaa tttattacgc
     2461 cgttgacctt tgcggctttg accggacaaa aagtaattac cgacctgttt ggcaattccc
     2521 aagatgaagc gaatctggaa agcgccatcg agcatatcgc cgtggcgcag cgtacggatc
     2581 tgttgctggt ggctcctgcg actgcggaca ttattgctaa attcgcgcgc gggattgctg
     2641 acgattttct gactacgttg tatctggcct cgaccgcgcc agtcgtggtg gctccggcga
     2701 tgaacgtgaa tatgtggaat catccggcga cgcaagaaaa tattgcgaag ctgcgggaac
     2761 gcggcgtgaa tgtcgtgcag cccgatgaag gttatctggc ttgcggcatg gtgggggcgg
     2821 gaaggttagc tgggcatcag gagattttgg cagcggtgcg cgcgaccttg cattcgaata
     2881 aagatttagc cggcgagacg ttgttggtta ccgcggggcc aacctgcgaa gaccttgacc
     2941 cggtccgcta tctgacgaat cgctcttccg ggaagatggg atatgcggtg gcggaagcgg
     3001 cggcgcggcg tggcgcaaaa gtgattctgg tcagtgggcc tacagcgctg gaaacgccgc
     3061 ttggcgttcg acgcgttgat gtgcgtaccg ccgagcagat gcatgaagcg gtgacgaaag
     3121 agtttgcagc atgctcgatt ggaatttttg cggctgccgt ggcggattac cggcccgcgg
     3181 aaaagcatgc agagaaaatc aaaaagaacg atgggaattt gacgatacat ttggaaccga
     3241 ccgtggatat tttgcaggat gtcgccaat
//
LOCUS       DATCBB010000103         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_132909, whole genome shotgun sequence.
ACCESSION   DATCBB010000103 DATCBB010000000
VERSION     DATCBB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132909"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VII37_01215"
     CDS             <1..688
                     /locus_tag="VII37_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5068365.1"
                     /translation="EDLSAKAEKLVSKLGVEVMKGVMVTNIDGNGVTFRRGDKTESLA
                     AHTVLWAGGVETTTFGKKLAQRTHAETDRSGRIKVNSDLTIPNYPDIFIVGDLAHAVE
                     KDGKPMPGVAQVAIQGGAYAAKTIKARIQNKKAEAAFHYFNKGDMAVIGRAAAVANIF
                     GFHLSGFIAWFTWLFVHLMYIVEFQNRVMVFIQWGFEYLTFSRGARLITGAAATDSIK
                     QARSISGDEN"
     gene            1039..2208
                     /locus_tag="VII37_01220"
     CDS             1039..2208
                     /locus_tag="VII37_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068366.1"
                     /translation="MPVTKKTFATTKNSITKKFATKKNLLASLATLFLLSVASPATFS
                     ATVNSQVCFLSSGTWANTSLAQAQSGTFRITFDAVPSASIVDAVHGLSYGPASAYANM
                     FVAVRFNASGYLDARNGSTYSAASAIPYAGRVSYHFILDVNVATHSYNAYVMIGSVQT
                     TIGRNLAFRNQLALTSSLNDIAAMTTTGSSTMCNITLSNSSMAPSITTQPASQSVTAG
                     QMATFSVATTGSALTYQWMKNGAVIAGANSSNYSTPVTTIADNGAQFSVSVSNGSGTA
                     SSNSALLTVTAPIIAPAITLQPMAQSVTAGQTANFSVATTGTAPMTYQWSRNGSPIIG
                     ATSSLYTTALTVVSDNGSQFTVLVSNSAGNVVSIPATLTVKAVSLPPGCLLSAGT"
     gene            2230..3195
                     /locus_tag="VII37_01225"
     CDS             2230..3195
                     /locus_tag="VII37_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEY5068367.1"
                     /translation="MQTSSFRIQFDVTPVAAIEDGVTGLSAAPAATYNDLAAAVRFNP
                     TGTIDARNGGSFVAATAIPYTAGTAYHFILDVNVGTHTYNAYVMIGSVQATIGTNLAF
                     RTQQASVSSLAYLNGLTVTGSDTICNAVTTTSTAATLLLNSSVSSLNFGNVNVSSSTT
                     QNITMTNAGNSSVTIAQVMVAGAGFNSTGASGITLALGQTTTLTSTFAPSANGAATGK
                     ITVSSNATNSPSSISLSGMGVTATTHSVALAWDPAVTGVTGFKTYVSVVHGGPYVEVG
                     AVLASTPVYNDSSVQSGHTYFYVVTAVNSTNQESGYSNEVAAIVP"
     gene            3332..3556
                     /locus_tag="VII37_01230"
     CDS             3332..3556
                     /locus_tag="VII37_01230"
                     /inference="COORDINATES: protein motif:HMM:NF039402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HEY5068368.1"
                     /translation="MLAIRLPQSIEKRLENLARRTGRTKTYYVREAILEHLEDIEDLY
                     LAERALERVRNDEEKAIPLKEVMKRRGSQR"
BASE COUNT          902 a         1123 c          856 g          721 t
ORIGIN      
        1 ggaagatctt tccgccaaag ccgaaaagct cgtctccaaa ctcggcgtcg aagtcatgaa
       61 aggcgtaatg gtcaccaaca tcgacggcaa cggcgtcacc ttccgccgcg gcgacaaaac
      121 cgaaagcctc gcagcacaca ccgtcctctg ggctggtgga gtcgagacca ccaccttcgg
      181 gaaaaaacta gcccagcgca ctcacgccga aacagacaga agcggccgca tcaaagtaaa
      241 ctctgatctc accatcccca attatcccga catattcatc gtcggcgatt tagcccacgc
      301 cgtggaaaaa gacggcaagc ccatgcccgg cgtcgcgcaa gtcgctatcc agggcggagc
      361 ttacgcggcc aaaaccatca aggcccgtat ccaaaacaaa aaagccgagg ccgccttcca
      421 ctatttcaac aaaggcgaca tggccgtaat cggccgcgcc gccgccgtgg ccaacatttt
      481 tggtttccat ctctcaggat tcatagcctg gttcacgtgg ctattcgtcc atctcatgta
      541 catcgtggaa ttccaaaatc gcgtaatggt cttcattcag tggggatttg aatacctgac
      601 tttcagccgc ggcgcacgcc taatcaccgg cgccgccgcc accgattcca tcaagcaggc
      661 ccgctcaatc agcggagacg agaattaaat ccgcggccaa gccaaacctc agtccgcaaa
      721 aaccgctcca caaaaaaatc cacgcaacaa aaagtttaca gaacaaaaag ttacatcaga
      781 aaaaaagcgg cgtcagcgaa atccaactgt acaagaaaac ctgcccactg gtctcagcct
      841 gtccctcagc ccacgaatct taataactct ccaatctgtt tttcactgaa cgcattctga
      901 ctccgccacc agtagttgcg aacaacacca aaccccattc cgcatttgct tcggcccccc
      961 ctctgcacct ccgagcaaac aaatcaggcc ggcttcccaa ggaagcccgg cacaaacggg
     1021 atcggaatgg ggactatcgt gccagtcaca aaaaagactt tcgccacaac aaagaattcg
     1081 atcacaaaaa aattcgccac aaaaaagaat ctactcgcat cgctcgctac cttgtttctg
     1141 ctatctgtcg cctcgcctgc taccttcagc gccacagtta attcgcaggt ttgcttcctg
     1201 tcgtccggca catgggctaa cacctcgttg gcgcaagccc aatccggaac cttccgcatt
     1261 acctttgacg ccgtaccttc cgcttccatc gtggatgcgg tgcacggcct gtcttacggc
     1321 cctgcttcgg cgtacgccaa catgtttgta gcggtgcgct tcaatgccag cggctacctt
     1381 gatgcgcgca acggttccac atactcagcg gcgtcagcaa ttccttacgc cgggcgcgtc
     1441 agctaccatt tcattctcga cgtgaacgtc gcgactcaca gttacaacgc ctacgtaatg
     1501 atcggctcgg tgcaaaccac catcggcaga aatcttgcgt tccgcaatca actcgccctg
     1561 acgtcatctc ttaacgacat cgccgcgatg accaccacag gcagcagcac catgtgcaac
     1621 attactttga gcaactcttc gatggctcca tccatcacca cgcagcctgc gagccaatcg
     1681 gtcactgctg gtcagatggc tacgttctct gtcgccacca ccggatcagc tctgacgtat
     1741 caatggatga agaacggtgc ggtaatcgct ggcgccaact cttctaacta ctccacaccc
     1801 gtaacaacca tcgccgacaa cggcgcgcag ttcagcgtct cagtcagcaa tggctccgga
     1861 acagcatcga gcaactcggc gctactcacc gtcactgcgc caatcattgc gccggcaatt
     1921 acattgcaac cgatggccca gtccgtgact gcaggacaaa cagcaaattt ctccgtggcc
     1981 accaccggca cggcaccgat gacgtatcaa tggagcagaa acggctcacc catcatcgga
     2041 gcgacttcct ctctgtacac gaccgcgttg acggttgttt ctgacaatgg atcgcagttt
     2101 accgtccttg ttagcaacag cgcaggcaac gtggtcagca tcccggcaac tctgaccgtg
     2161 aaagcggttt cactaccgcc gggatgcttg ctgtcagcgg gcacgtgagt ttctacaccg
     2221 ctaacacaag tgcagaccag cagcttccgc atacagttcg acgttacgcc agtggctgcc
     2281 atcgaagacg gcgtcaccgg cttgtccgcc gcgccagccg ccacatataa cgatttggcc
     2341 gccgcagtgc gcttcaatcc aaccggaacg atcgacgcca ggaacggtgg cagcttcgtg
     2401 gccgccacag caattcctta caccgcaggc actgcgtatc atttcattct cgatgtgaat
     2461 gtcggaacac acacctacaa cgcgtatgtc atgatcggtt cagtgcaagc caccatcggc
     2521 acgaacctgg cattccgcac acaacaagct tccgtttctt cgcttgctta tctgaatgga
     2581 ttgacggtta cgggcagcga tacgatttgc aacgcagtga cgacgacttc gaccgcggcg
     2641 actctgttgc tcaactccag cgtaagcagc ttgaacttcg gaaacgtgaa tgtgtccagc
     2701 agcactaccc agaacataac catgaccaac gctggaaatt ccagcgtgac gattgcgcaa
     2761 gtaatggttg cgggcgccgg cttcaactcc accggagcga gcggcatcac gcttgcactg
     2821 ggtcaaacta caactctgac ttccacgttc gcaccttccg cgaacggagc agccaccggc
     2881 aaaatcacgg taagcagcaa cgccaccaat tctccttcca gcatctcgct gtcgggaatg
     2941 ggcgtcaccg ctaccactca cagtgttgcg ctcgcgtggg atcctgcggt tactggcgtg
     3001 acgggattca agacttacgt aagcgtggtt cacggcgggc cgtacgttga agtaggagca
     3061 gtgctagcaa gcactccggt gtacaacgat agcagtgtgc agtcgggaca cacttacttc
     3121 tacgtagtca cggcggtaaa ttccaccaac caggaaagcg gctactccaa cgaagtggcc
     3181 gcgatcgtgc cgtaaaaaat agtacagcta gatataaaag ccggaggcac gcgaaagcgt
     3241 gtttccggca tctcctttat ataagcttca aaagttatgt atttgtgacg caggcttcac
     3301 caatttgcta tacttgtata gcgaggtagt tatgctggcc attcgacttc cgcagtccat
     3361 cgaaaaacgc ctggagaatc tcgcccggcg caccggtcgc actaagactt actatgtccg
     3421 tgaagccatc ctcgaacacc tagaggatat cgaggacctg tatctggccg aacgcgcgct
     3481 ggaacgggtc cgcaacgatg aagagaaagc tattccattg aaggaagtca tgaagcggcg
     3541 tggctcacaa cgttgagctg tcagagtcag cggaccggga actcggcaag ctcgacgtgc
     3601 ag
//
LOCUS       DATCBB010000104         1671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_425034, whole genome shotgun sequence.
ACCESSION   DATCBB010000104 DATCBB010000000
VERSION     DATCBB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425034"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1671)
                     /locus_tag="VII37_01235"
     CDS             complement(<1..>1671)
                     /locus_tag="VII37_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068369.1"
                     /translation="TRTQNVQLDLGAVNQTVEVTSEGSVTLNTTDSTIGNNFDLRAVA
                     SLPIEFRDDPSALLRLQPGVVNAQSEQTGANSASDPSGSRDGSVAGARADQNNITVDG
                     IDATDFTAGQSFRTQAAIPVESVQEFSTQVADITPAYGGRGGAQTIITTKSGTNDWHG
                     SAHEYNRTALTEANTFFNNAAGVPRTNLVRNQFGADLGGPVKHDKLFFFFDYEGRRDA
                     QQINQLQLVPLPHVKTGQIAYINATSTTTGNPCSTNSRLTSQDVSTDFVTILPAAQVA
                     ALDPCSQVGGCPNAPGFAAPGVAPALQTLFQSRYPTPNDFSSGDGINTAGFRFNAPNP
                     LTENSYLGRFDFNISQKHKLFGRVNLRNQDSINQFIQFPGDPLTSPNTIRDYGWVIGE
                     TWAISPRTVNQLIIGETRSNLDQPILFNPSGGLVELSFFGGALSNPFVRQSQIGHISP
                     VPTFRDDLTLLRGRHTIQLGVEWNPTKVRSTLTNNFDFIQLGIGGSISSLPTNLRPAN
                     LLQDPGGIAAANWDNFFVGALGIINNDQSAITYNRDGSVVPQGTETHRR"
BASE COUNT          352 a          371 c          570 g          378 t
ORIGIN      
        1 gcggcggtgc gtttccgtgc cttgtggaac gacgctgccg tcgcggttat aggtgatggc
       61 gctctggtcg ttgtttatga tgccgagcgc gccgacgaag aagttgtccc agttggcggc
      121 cgcgatgccg ccgggatcct gaagaagatt ggcgggacgc agattggtgg ggagactgga
      181 gatgctgccg ccgataccta actggataaa gtcaaaattg ttggtcaaag tgctgcggac
      241 ttttgtggga ttccactcca caccgagctg gatggtgtga cggccgcgaa gcaaagtgag
      301 gtcgtcgcgg aaggtgggaa ccggactgat gtggccgatc tggctctggc gcacaaaagg
      361 gttggacagc gcgccgccaa agaaggatag ctccaccaaa ccgccggagg gattaaagag
      421 aatcggttga tcaagattcg agcgcgtctc tccgatgatc aattgattga cggttctggg
      481 actgatggcc caggtttcgc cgatgaccca gccgtaatcg cggatagtgt taggactagt
      541 gagagggtcg ccgggaaatt gtatgaactg attaatagaa tcctggttgc gcaaattcac
      601 gcggccgaac aatttgtgtt tttgacttat gttgaaatcg aagcggccta gataggaatt
      661 ttcggtgagc gggttagggg cgttgaagcg gaaaccggca gtgttgattc cgtctcccga
      721 ggagaaatcg ttaggagtgg ggtagcgact ctgaaacaag gtctgcaggg ctggagcaac
      781 gcccggagca gcgaagcctg gggcgttagg gcaaccgccg acctgtgagc agggatcgag
      841 ggcggcgacc tgagcagcgg gaagaatagt tacgaaatcg gtgctgacgt cttgcgaggt
      901 cagccggctg ttagtggagc aaggatttcc ggtggtggtg ctagtggcat tgatataagc
      961 gatctgaccg gtctttacgt gaggcaaggg caccagctgg agctggttta tctgttgcgc
     1021 atcgcgacgg ccttcatagt caaagaagaa aaacaatttg tcatgtttta cggggccgcc
     1081 tagatcggcg ccgaactggt tgcggactaa atttgtgcgc gggacgccag cggcgttgtt
     1141 gaaaaaagta ttggcttcgg taagtgcggt gcgattgtat tcgtgcgcgg aaccgtgcca
     1201 gtcgtttgtt ccactttttg tggtgatgat ggtttgggcg ccaccgcgac cgccgtaagc
     1261 gggggtaatg tctgctactt gggtactgaa ttcctgaacg gattcgacgg gaattgcggc
     1321 ttgggttctg aaggactggc cagcggtgaa gtccgtggcg tcgataccat ccacggtgat
     1381 gttattttga tcggcacggg cgccggcgac agaaccatcg cggctgccgc tgggatcact
     1441 ggcgctgttg gcgcccgttt gttcactttg cgcgttaacc acgccaggct gcaaacggag
     1501 caaagcagag gggtcatcac ggaattcgat aggcagggag gcgacggcgc gcagatcgaa
     1561 attgttgccg atggtgctgt cagtggtgtt cagcgtgact gagccttcgg aggtgacttc
     1621 gacagtttgg ttgacggcgc caagatccag ttgaacattt tgcgtacggg t
//
LOCUS       DATCBB010000105         1580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_262969, whole genome shotgun sequence.
ACCESSION   DATCBB010000105 DATCBB010000000
VERSION     DATCBB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262969"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>1580
                     /gene="lptD"
                     /locus_tag="VII37_01240"
     CDS             53..>1580
                     /gene="lptD"
                     /locus_tag="VII37_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY5068370.1"
                     /translation="MADLSSRGPQSHRGDLFIADDDVDIHYGDSRLRADHVEYNEKTS
                     EAFARGHVQFDFENQHIEAEEAHYNVKTGHGVFHHVRGTVKIERRPNPNVLLSDNPLY
                     FEARDVERFAKDVYVIDQAWITICDAEHPTWQFFAPHARVQLEKTVAMVNANFRLFRV
                     PLIWLPYATAPAGRKVRESGFLIPDIGESSRKGFTLGDAFYWAPKPWFDATLGAQYMS
                     SRGVMERGTLRVKPFEGTSINYTYFGVDDRGLIVGGVRQPQGGEEQRLEMQSQLPRGW
                     RFVTDYNQLSSLTFRLAFADTYGDAINSEVRSAVFLSNNFRGFSLNFAGLNDKSFLQI
                     NPTSSVSIRNFPEARFDSVEQAPWRNLPIYFGFDSFVDALHRADSEINTPSFVGRTEF
                     APRVTVPIHVGEWLGITTTAAFRTTRYGASLTAADEVSNQAIVRNTGEFTVDLRPPTL
                     ERFFERPKSKRRYKHSVEPIITYRYVTGVNNFADFIRFDSDATITDTNEVEFGITQRF
                     F"
BASE COUNT          363 a          401 c          458 g          358 t
ORIGIN      
        1 gccaagaacg gccagcgcgc agcaagcgca tcttccattt aaaaccggcg gcgtggctga
       61 tctttcgtct cgtggtccgc aaagccatcg cggggatttg tttatcgcgg acgatgatgt
      121 ggatattcac tacggggatt cgcgattacg cgcggatcat gtggaataca acgagaagac
      181 ctctgaggcc tttgcgcggg gccacgtgca atttgatttt gagaaccagc atattgaggc
      241 cgaggaagcg cattacaacg taaaaacggg tcacggtgtt ttccatcatg tccgaggcac
      301 cgttaaaatc gagcggcggc cgaatcccaa cgttttgctg tcagacaatc cgctttactt
      361 cgaagcgcgg gacgtagagc gcttcgcaaa ggatgtttat gtaattgacc aggcgtggat
      421 caccatctgc gatgcggaac atccgacgtg gcaattcttt gcgccgcacg cgcgagtgca
      481 actggaaaaa actgttgcca tggtgaacgc aaattttcga ctgttccgcg tcccactcat
      541 ttggctgccg tatgccacgg cgcctgcggg ccgcaaagtg cgcgaatccg gatttctgat
      601 cccggatata ggggagagtt cgcggaaggg atttacgctc ggcgatgctt tttactgggc
      661 gcccaagccg tggtttgacg cgacgctggg agcgcaatat atgagttcac gcggagtgat
      721 ggaacgaggc acgctgcgcg tcaagccctt tgaaggcacg tcgatcaact acacatattt
      781 tggcgtcgat gatcgcggtc taatcgtcgg cggagtgcgg cagccacagg gaggcgagga
      841 gcagcggctc gaaatgcaat cacagttgcc acgcgggtgg cgattcgtca cggactataa
      901 ccagctttcg tcgctgacgt tccggctagc gttcgcggat acttacggag acgctatcaa
      961 ctccgaagtg cgcagcgcag tttttctcag caacaatttc cgcgggttca gcctgaactt
     1021 cgccggtttg aacgacaaaa gttttttgca gataaacccg accagcagcg tcagcattcg
     1081 caattttccg gaagcgcgct ttgattccgt agagcaagcc ccatggcgca atttgccgat
     1141 ttattttggg tttgacagct ttgtggatgc gctacatcgt gccgacagcg aaatcaatac
     1201 gccaagtttt gtgggccgca cggaatttgc gccacgagtg acggtgccga ttcatgtggg
     1261 agaatggcta ggcatcacaa ctaccgcggc gtttcgaacg acgcgttacg gggcttctct
     1321 gacggcggcg gacgaggtga gcaatcaagc gattgtgcgc aacaccgggg agtttacggt
     1381 ggacctgcga ccgccaacgc tagaacgttt ctttgagcgt ccaaaatcaa agagaagata
     1441 taagcactcg gttgagccga taattaccta tcgctacgtg acgggcgtga acaattttgc
     1501 ggatttcatt cgattcgatt ccgacgccac gattacggac acgaacgaag tagagttcgg
     1561 aatcacgcaa cgtttttttg
//
LOCUS       DATCBB010000106         4097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_425100, whole genome shotgun sequence.
ACCESSION   DATCBB010000106 DATCBB010000000
VERSION     DATCBB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425100"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VII37_01245"
     CDS             <1..1757
                     /locus_tag="VII37_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5068371.1"
                     /translation="SVWSIAWTGGEPKKLDAGDSPQISPTRTVAYIKDHQLWVAKLDA
                     SDKPFQIVVRGNSADPQWSPDGTKLAFVSSRTDHAFIAVYDPSNKSLRYLSPSVDSDQ
                     TPRWSPDGKFIAFIRRPAALRDKPEGFFIAPDVPELWAVWIADVSSSSAHEIWRSGKE
                     LTDSFPAMAADTGGGVLNWGSNDRLIIASAKDGWQHLYSLAADAKKQEKLTLLTPGNC
                     EVEQWSFSPDKQTIVFNSNCGRAKEDIDRRHLSRVDVAGGSEPVRITTGESIEWSPVV
                     LGDGKSIAYLGSDARHPGRPFVSPLAFDFSPRWLDSGTWPADFPADHLVAPQQMVFHS
                     GDGLELHAQLFLPPNLKPGEKRPALVFLHGGPMRQMLLGWHYMYYYSNCYAMNQYLAS
                     RGYIVMALNYRSGIGYGHSFFEAPGRAGRGASEYQDVVAAGKFLAARSDVDPKHIGLW
                     GGSYGGYLTALGLGRNSDLFAAGVDLHGVHDWPTDNWDGKNIPPELTKLAHTSSPVTA
                     VDTWRSPVLFIHGDDDRNVIFTQTVDLIARLRAKNVEIEQLIFPDEIHDFLLHRSWLA
                     AYHAASDFFDRRFGGPWK"
     gene            1815..2795
                     /locus_tag="VII37_01250"
     CDS             1815..2795
                     /locus_tag="VII37_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068372.1"
                     /translation="MLILATLFSTAEVKHSVAAAPIARATLFPRLHAGQTFTYFVQYR
                     TKKNVKTESRVVTPTGPQDAETDAQWLLHVDILDVHPQGERAAIHARSQFQSVASAMA
                     QNNAGAQPTSSGSPAQNVETKSVDFTILPDGRVDAVKGLAELFPDQRQVWQVWLRQFA
                     IAGVFPRDGVRPGQSWKSNEPEQAPSPIVKLEWEKRATYVRDEPCTPIQFSETGIGTP
                     KDSPSESCAVVLTTAVLKQKSSPKDTTPGDFRLHDLRTTGNATGKNETISYISLRSGL
                     IVRVTEDAQQFMDVVVAKTDASNQIHYNVEATGHTEVLLVADAPASKPKP"
     gene            2966..>4097
                     /locus_tag="VII37_01255"
     CDS             2966..>4097
                     /locus_tag="VII37_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY5068373.1"
                     /translation="MKFKLHSNYQPRGDQPEAIDQLYRGLEAGDKEQVLLGVTGSGKT
                     FTMAKLIEAANRPALVLAHNKTLAAQLYHEFKSFFPENAVEYFVSYYDFYQPEAYVPS
                     ADIYIDKEATINDELDKLRMSATRSLFERTDVIVVASVSCIYGIGSPEAYFGQIVMLE
                     KGQQISREAMLHRFVDIQYERSEDLRRGTFRVRGDTIEIYPPYQDQGYRIEMWGQQIE
                     ALRQIDPLTGEIRAGQEDLSRVSIYPKTHYVVPQDQKERAIQSIYAELEWWKKELEKQ
                     GKFVEAQRVTQRTLFDIEMMRTVGYCHGIENYSRHLSGRLPGEAPPTLLDYVPQDFLM
                     FIDESHQTVSQVRGMFKGDRSRKQTLVDYGFRMPSALDNRPLT"
BASE COUNT          925 a         1223 c         1044 g          905 t
ORIGIN      
        1 aatccgtttg gtcgatcgca tggaccggtg gcgagccaaa aaaactggac gccggtgatt
       61 ctccgcagat ttcccctacg agaaccgtag cctatattaa agaccaccaa ttatgggtgg
      121 ccaaactcga cgcatccgac aagccgtttc aaattgtcgt gcgcggcaac agcgccgatc
      181 cgcagtggtc gccagacggc accaaacttg ccttcgtttc atcgcgcacc gatcacgcct
      241 tcattgccgt ctatgaccct tcaaataaat cgttgcgtta tttatctccc tccgtcgatt
      301 ccgaccagac tcctcgctgg tcacctgacg ggaaattcat cgcgtttatc cgtcgaccgg
      361 ccgcgctgcg cgacaaaccc gaagggtttt tcatcgcgcc ggatgtgcca gagctctggg
      421 ctgtttggat cgccgatgtt tccagctcct ccgcgcatga aatctggcgc agcggaaaag
      481 aactgaccga ctcctttccc gcgatggctg cagacaccgg aggcggagtg ctcaattggg
      541 gatccaacga ccgccttatc atcgcgagcg ctaaagatgg ctggcagcat ctatactcgc
      601 tggccgcgga tgcaaaaaaa caggaaaaac taactcttct cacgcccggg aattgtgaag
      661 tggaacaatg gtcgttctct cccgataagc aaaccatcgt gttcaattcc aattgtggca
      721 gggccaaaga agatatcgat cgccgccacc tttcgcgtgt tgacgtcgcc gggggcagcg
      781 aacccgtccg catcactacc ggcgagagta ttgaatggag tccagtcgtt ctcggcgatg
      841 gcaaatccat cgcttattta ggctccgacg cgaggcaccc cggtcgccct tttgtttcac
      901 cgctcgcctt cgatttttcg ccgcgctggc tggattctgg tacgtggcca gctgactttc
      961 ccgccgacca tctcgtcgct ccgcagcaaa tggtttttca ttctggagac ggtctcgagc
     1021 tacacgctca attatttttg ccgccgaatc tcaaacctgg tgagaagcgt ccagccctcg
     1081 ttttccttca tggcggcccc atgcgccaaa tgctcctggg ctggcattac atgtactact
     1141 actcgaattg ctacgccatg aaccagtact tggccagccg cggttatatt gttatggccc
     1201 tgaattaccg cagcggcatc ggctatggcc actcattttt tgaagcgccg ggccgcgctg
     1261 gacgtggcgc ttcggaatat caggacgttg tggctgctgg gaaatttctt gccgcgcgca
     1321 gtgacgtcga tcccaagcac attggcttat ggggcgggag ctacggcggt tatcttaccg
     1381 cgctcggcct cggccgcaat tccgatttgt tcgccgcggg cgttgatttg cacggcgtgc
     1441 acgactggcc cacggataat tgggacggga aaaacattcc acccgaactc accaaactgg
     1501 cccacacctc ttcgccagtc accgctgtcg acacctggcg gtcgccggtg ttattcattc
     1561 acggcgatga tgatcgcaac gtgatcttca cccaaactgt ggacctcatc gcgcgcttac
     1621 gagcgaaaaa cgtcgaaatt gagcagctga ttttccctga cgagattcac gattttcttt
     1681 tgcatcgaag ttggttggct gcttaccacg ccgccagcga tttctttgac cgccgcttcg
     1741 gcggtccgtg gaagtgattt ctgaaaagta gaatctcgag aatcggatgc gcggatcgcc
     1801 tcatcatttg ttttttgctg atattggcga cattatttag cactgctgag gttaagcaca
     1861 gcgtcgccgc cgcgccaatc gcacgcgcca cactgtttcc ccgcctgcat gccggccaaa
     1921 cgttcaccta cttcgttcaa tatcgcacca aaaaaaacgt caagaccgaa agtcgcgtcg
     1981 ttactcctac tggaccacaa gacgccgaga cggacgccca gtggctcttg cacgtagata
     2041 ttttggatgt gcatcctcaa ggcgaacgcg ccgccattca cgcccgctcc cagtttcaaa
     2101 gtgtcgcgtc cgctatggcc caaaataatg ccggcgccca accaacctcg tccgggtcgc
     2161 ctgcgcagaa cgtggaaacg aaatccgtgg acttcaccat tctgcccgac ggccgcgtgg
     2221 atgcagtgaa aggtctcgcc gaactttttc ccgaccagcg ccaggtttgg caagtttggc
     2281 tacgacaatt cgccattgct ggtgtttttc cgcgcgacgg tgtgagacca ggccagtcct
     2341 ggaaatcgaa cgaaccagag caagctccgt cgccgatcgt aaaactcgag tgggaaaaac
     2401 gggccaccta cgttcgagat gaaccctgca ctcccattca attctccgaa acgggcatcg
     2461 gcactccaaa agattctccc agcgaatcat gcgcggtggt cttgaccacc gccgtcttga
     2521 aacagaaatc ttctccgaaa gatactaccc ccggcgactt caggcttcat gaccttcgca
     2581 ccaccggcaa cgccaccggc aaaaacgaaa ccatctcgta catctcgctg cggtccggcc
     2641 ttatcgtccg cgtcacggaa gacgcgcagc agttcatgga tgtggtcgta gccaaaaccg
     2701 acgcatccaa tcaaattcac tacaatgtgg aggccaccgg ccacactgag gtcctgctgg
     2761 tcgcggatgc gccggcgtca aaaccaaaac cctgaagcgc gaagtacgtc gcgctgtcgt
     2821 caccccgcct ggaacaagaa atccctgttc tgaaaaggtt tgcgtgatcg cgtataatgg
     2881 aggtttcggc agtccccaag aaagttatcc ggtgcacata gggtgactgc acttaattca
     2941 gttccacact aaggaacgcc tgcaaatgaa gttcaaatta cacagcaatt atcagccgcg
     3001 gggcgaccag cctgaagcga tcgaccaact ttatcgcggt ctggaagccg gcgacaaaga
     3061 acaggtgttg ctgggcgtca ctggctccgg caaaaccttc accatggcca agctcatcga
     3121 ggccgcaaat cggcccgctc tggtgttggc gcacaataaa actctcgcgg cccagctcta
     3181 ccacgagttc aaaagttttt ttccggaaaa cgccgtcgaa tatttcgtca gctattacga
     3241 tttttaccag ccggaagctt acgtgccctc cgccgacatc tacatcgata aagaagccac
     3301 catcaacgac gaattggaca agttgcgcat gagcgccacg cgctcgctct tcgaacgcac
     3361 tgacgtgatc gtggtggcct ccgtctcctg catctatggc attggttcgc cggaagcgta
     3421 tttcggccag atcgtcatgc tggaaaaagg ccagcaaatc tctcgcgaag ccatgttgca
     3481 tcgcttcgta gatattcagt acgaacgctc cgaagatctt cgccgcggta cctttcgcgt
     3541 gcgtggcgac accatcgaaa tctatccgcc ttatcaggac cagggctatc gcatcgaaat
     3601 gtggggtcag cagattgaag cgctgcgcca gatcgatcct ctgaccgggg agattcgtgc
     3661 tggtcaggaa gatttatcgc gcgtttccat ttatcccaaa acccattacg tcgtgcccca
     3721 ggaccaaaaa gagcgcgcca tccaaagcat ttatgccgag ctggaatggt ggaaaaagga
     3781 gctggaaaag caggggaaat ttgtagaggc ccagcgcgtc acacaacgca ctcttttcga
     3841 tatcgaaatg atgcgcaccg tcggctattg tcacggaatt gaaaattatt ctcgccattt
     3901 gagcggccgc ttgccgggcg aggctccgcc aacgctgctc gattacgttc cgcaagattt
     3961 tttgatgttc atcgatgaat cgcatcaaac cgtcagccag gtgcgcggaa tgttcaaagg
     4021 cgaccgctcg cgcaaacaga ccttggtgga ctacggattc cgcatgccca gcgcgctcga
     4081 caatcgcccg ctcacct
//
LOCUS       DATCBB010000107         1004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_425108, whole genome shotgun sequence.
ACCESSION   DATCBB010000107 DATCBB010000000
VERSION     DATCBB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425108"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VII37_01260"
     CDS             complement(<1..403)
                     /locus_tag="VII37_01260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5068374.1"
                     /translation="MIGQTISHYRIVEKLGGGGMGVVYKAEDTELGRFVALKFLPDDV
                     ARDPQSLERFRREARAASALNHPNICTIYEIGKDGEQSFIAMEFLDGVTLKHQIDGRP
                     LDIEKLLPIAIEIADALDAAHGQGIVHRDIKP"
     gene            608..>1004
                     /locus_tag="VII37_01265"
     CDS             608..>1004
                     /locus_tag="VII37_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068375.1"
                     /translation="MMRLSGRVNNPGYRVATAKLAVAGAILLAVAAAAPGARAEDVTK
                     SFTVNGRAIVRVDTNDGSVRVTSGDNKEVQFRVEYSGYELNRNLRVDTRQDGDKVELV
                     ARVNGHWGFWGGHSRNLRIEVRMPKSGDLQ"
BASE COUNT          206 a          233 c          341 g          224 t
ORIGIN      
        1 cgggcttaat gtcgcgatga acaattccct gcccgtgggc ggcgtcgagc gcgtcggcga
       61 tttctatggc gataggaagg agcttttcga tgtccagcgg acggccatcg atttgatgct
      121 tgagagtgac gccatccaga aattccatgg cgatgaagct ttgctcgcca tcttttccga
      181 tttcataaat ggtgcaaatg ttgggatggt tcaatgcgga ggcggcgcgg gcttcacggc
      241 gaaaacgctc gagggattgc gggtcgcgcg caacatcgtc gggcaggaat tttagggcga
      301 cgaagcggcc cagctcggtg tcttcagctt tatagactac gcccatgccg ccaccgccta
      361 gtttttcgac gatgcggtag tgggagatgg tctggccaat catgtggtgg gcgtggtttg
      421 cgcagccatg ttacgggtgc ggagcggctt cgtcaacgcg ggttcgaaat cgtggttacc
      481 agacttaggc tggactttgc cattccaggg aacttatgcc ggatagctca cgtatcctaa
      541 tatggcaggg caataccggc gtagacccag atgcgtgagc gagaatttgg tccacggagg
      601 actacaaatg atgcgacttt ctggacgagt taataacccg ggttatcggg tggcaacggc
      661 caagctagcg gtggctgggg cgattctatt ggccgttgct gcggctgcgc ctggcgcacg
      721 ggccgaagat gtgacgaagt cgtttaccgt gaacgggcgc gcgattgtac gcgtggacac
      781 caatgatggc agcgtgcggg tgacgagcgg ggacaataaa gaggtgcaat tccgcgtcga
      841 atattcgggt tacgagctca acaggaattt gcgcgtggat acgcggcagg acggcgataa
      901 ggttgagctc gtggcgcgtg ttaacgggca ttggggattt tggggtgggc acagcagaaa
      961 cttgcgcatc gaagtgcgca tgcctaagtc tggcgatttg cagg
//
LOCUS       DATCBB010000108         1169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_457528, whole genome shotgun sequence.
ACCESSION   DATCBB010000108 DATCBB010000000
VERSION     DATCBB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457528"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VII37_01270"
     CDS             complement(<1..933)
                     /locus_tag="VII37_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5068376.1"
                     /translation="MSEKMHEGLKKEIATYEKRTPKSAEAHKRALQRIPLGVASNYRA
                     YDPYPIFVKKGEGSKLWDLDGNEYIDHNLCFGALMAGHCHPAVVKAIESALHIGTTFG
                     MPHDMEWELAEEICARYPVEMVRFGSSGTEVTMHACRMARAATGRDKILKFEGAYHGL
                     HDTALVSVKPKAEEYGDPNAPTSVPGGKGVPKASVDNVVIAAFNNLASVENRFKQFPG
                     QIAAIILEPILMNVGLCMPQPRFLQGLRDIATKNGSLLIFDEVKTGAKLAWGGASEFF
                     GVKPDMICLAKSIGGGAPLGAFGSSRAVMDLISQH"
BASE COUNT          269 a          351 c          279 g          270 t
ORIGIN      
        1 atgctgcgaa atcagatcca tgaccgcgcg gctggatccg aacgctccaa gtggcgcgcc
       61 accgccaatg gactttgcca ggcagatcat gtccggcttc acgccaaaaa actccgaagc
      121 tccgccccat gccaatttcg ccccggtttt cacttcgtca aaaatcagca gtgagccatt
      181 tttcgtggcg atatcgcgca ggccctgcaa aaagcgcggt tgcggcatgc acaatcccac
      241 gttcatcaaa atgggctcaa gaataatcgc cgcgatctgg ccgggaaatt gtttaaaccg
      301 gttttccacg ctggccaaat tattgaaagc cgcgatcacc acattgtcca cggaggcttt
      361 tggaacaccc ttgccaccag gcacggacgt tggagcgttc ggatcgccat actcttccgc
      421 tttgggtttt acgctgacga gcgcggtgtc atgcaaaccg tgatacgcgc cttcaaattt
      481 caaaattttg tcgcgcccgg tcgctgcacg cgccatgcgg caagcgtgca tggtaacttc
      541 cgtgccgctg gagccgaagc gcaccatctc caccgggtag cgtgcgcaaa tctcttccgc
      601 aagctcccat tccatgtcgt gcggcatgcc aaaagtcgtg ccgatatgca gcgcactctc
      661 gatggccttc actaccgcgg gatggcagtg cccggccatc aacgccccaa agcacaaatt
      721 gtgatcgata tattcattgc catcgagatc ccacaacttg ctaccttcgc cttttttcac
      781 aaaaatagga taaggatcgt aagcgcgata attgctagct actccaagcg gaatacgttg
      841 cagagcgcgt ttgtgggctt ccgcagactt aggcgtgcgc ttttcataag tggcaatttc
      901 tttcttcagt ccttcatgca ttttttccga catacattct ccgaacctgg cggcatcact
      961 gccaccaaca ggtctcttcc ctgcagtcag ttttccgtcg tgtaaaaaaa ttagaatcgg
     1021 ctgcgcgacc gaaactacct acagtctacc agcggggcat gcgctcgccg cggactggtc
     1081 tttagcatgt ctgcaaatcc tcagaacaga aacttcagcg caaactggaa caatcgtggc
     1141 ggtcgcacct tctgcgcaaa tccgaagtt
//
LOCUS       DATCBB010000109         1327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_295614, whole genome shotgun sequence.
ACCESSION   DATCBB010000109 DATCBB010000000
VERSION     DATCBB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295614"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VII37_01275"
     CDS             complement(<1..394)
                     /locus_tag="VII37_01275"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY5068377.1"
                     /translation="MYPILFHIRNFPINTYGVFLALAFLGAIFVTVKLAGRDGLPRER
                     IYDLCLWLLLSSLIGSKLLMFFTEPEYREHPLQLFSLDFLRSGGVFYGGLLGALITGY
                     FLMRRWKLPWWKTADACAPGIALGNVLGR"
     gene            complement(406..957)
                     /gene="lspA"
                     /locus_tag="VII37_01280"
     CDS             complement(406..957)
                     /gene="lspA"
                     /locus_tag="VII37_01280"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5068378.1"
                     /translation="MTDSESQSSRKTRAAWRVGYLFAALGIYLMDQSSKAWAVRHLRF
                     GDRRIISGMVDFVYAENPGIAFGQLQEGGAFGRWFFVVLAAAAAVAVLVYFVRTSRTD
                     DRVLGACALLLAGILGNLTDRVRLGFVIDFILVHAGQYHWPTFNVADASICVGALLLA
                     YDLIFASRKAKETASPRPLTTDN"
BASE COUNT          350 a          389 c          350 g          238 t
ORIGIN      
        1 gccggcccag tacgttaccc agcgcgatgc ccggcgcgca ggcgtcggcg gttttccacc
       61 acggcaactt ccatcgtcgc atcaggaaat agccggtgat aagcgccccc agcagtccgc
      121 cataaaaaac tccgccggag cgcaggaaat ccagggagaa gagctgcagc ggatgctcac
      181 gatattcggg ctcagtgaag aacatcaaaa gctttgagcc gatcagtgaa gacagcaaca
      241 gccacagaca gagatcgtag atccgctcgc gtggcaatcc gtcgcgtccg gctagtttca
      301 ccgtgacgaa gatcgcgccc agaaaagcga gagcgagaaa cactccgtag gtgttaatgg
      361 ggaagttacg gatgtgaaag agaatgggat acatgaatgc agaaatcagt tgtcagtagt
      421 taacggccgg ggactcgcag tttcttttgc ctttctactt gcgaaaatca gatcgtaggc
      481 caaaagcaat gccccgacgc agatactcgc gtcagcaaca ttgaaggtgg gccagtgata
      541 ttgccccgcg tgcaccaaaa taaaatcgat gacaaatccc agccgcactc gatccgtcag
      601 gttccccaaa atcccggcca gcaacaaggc gcaggcgccg aggacgcgat cgtcggtgcg
      661 cgacgtgcgc acaaagtaaa cgagcaccgc cactgctgcc gctgccgcca gcaccacaaa
      721 aaaccagcgg ccgaacgcgc cgccttcctg taactggcca aacgcgatcc cgggattctc
      781 ggcgtaaaca aagtcgacca tcccgctaat gattctgcga tctccaaaac gcaaatggcg
      841 cacggcccag gccttgctcg actggtccat cagatagatg cccagcgcag cgaagaggta
      901 gccgacgcgc caggcggccc gagttttgcg agacgattgt gattcgctat cggtcattcc
      961 aatgatttat aagtgcgctt ggttgcaacg agcagccgcg tagcggcgaa atatttatgg
     1021 aacccaatgc ccaaaatgat cccagctccg gaggagcgaa acatttctgt cgcccataaa
     1081 tgggctccgg taaataaaag gggctctggt agctataaac attccgtgcc taacggcact
     1141 gagaacgaaa gccctcaggt tgcctcaatt tcggccagcg cttcaacaca acgttcacac
     1201 accgtcgaat agcgcgatga ttcgccgaca cgggtcgaat aattccagca acgatcgcat
     1261 ttctggccat cagcacggcg caccttgatg ctcacgtcac cagtctctgc ttgcgtcaac
     1321 gaaacct
//
LOCUS       DATCBB010000110         1094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_133167, whole genome shotgun sequence.
ACCESSION   DATCBB010000110 DATCBB010000000
VERSION     DATCBB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133167"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VII37_01285"
     CDS             <1..230
                     /locus_tag="VII37_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068379.1"
                     /translation="YAAIASEIFPGEIRAAAMGLSYNIGRGLSAAAPFAVGALALHYG
                     IGPAFSLLAAAFFIAALLALLLPETLGRALT"
     gene            complement(266..352)
                     /locus_tag="VII37_01290"
     tRNA            complement(266..352)
                     /locus_tag="VII37_01290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Leu,seq:tag)
     gene            423..>1094
                     /locus_tag="VII37_01295"
     CDS             423..>1094
                     /locus_tag="VII37_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5068380.1"
                     /translation="MSRDVRSLLQLYNPNNPLDHASTIPSPWYFDESIAELENSAVFC
                     KTWQAVGRTDQVQKPGQFLTADIAGDPIVVVRGEDSQLRAFYNVCRHHAAAVVTEAQG
                     CAKQFRCPYHGWTYGIDGALKGMVEFDGVCDFERSDNGLVPVQVDTWENFVFVNLDPD
                     AGSLGNFLGSIPPIVAPLELTKKLHYFDRRIYHLQCNWKVYVDNYLDGGYHVPHAHKG
                     LSSVIE"
BASE COUNT          225 a          265 c          327 g          277 t
ORIGIN      
        1 gctatgccgc catagcgagt gaaatatttc ccggggaaat ccgcgcggca gccatggggc
       61 tcagttataa catcggccgc ggactttcgg ccgctgcgcc atttgctgtt ggggcattgg
      121 cgctacatta cgggatcggg ccagcgttct cgttgctagc cgcggcattt tttatcgctg
      181 cgcttttagc gttgcttctt cctgaaactt taggacgggc gctgacctga tcgaagccag
      241 tgtagaactg attggatgga gttgatggtg cgggaagagg gatttgaacc cccaagggct
      301 tgcgcccggc aggtcctaag cctgctgcgt ctgccagttc cgccattccc gcgaagtgtg
      361 cagagaaagt ttatcatgct aatttatagc taaagaatag cgttgtgtgt ctggagccgt
      421 ttatgtcgcg agatgtgcgc agccttttac aactctataa tccgaataat ccgttggacc
      481 atgcgtccac cattccttcg ccttggtatt tcgatgaaag tatcgcagaa ctagagaatt
      541 cggctgtttt ctgcaaaacg tggcaggcag tggggcgcac cgaccaggtt cagaagcccg
      601 ggcaatttct gactgccgat atcgcgggcg atcccatcgt ggtggtgcgc ggtgaagact
      661 cgcaattacg cgctttctat aacgtgtgcc gtcatcatgc cgcggcggtg gtgacggaag
      721 cgcaaggctg cgcgaaacag tttcgctgtc cttatcacgg gtggacgtat gggatcgatg
      781 gcgcgttgaa agggatggtt gaattcgacg gtgtgtgcga ttttgagcgc tccgataacg
      841 gactggtgcc cgttcaggtg gacacctggg agaatttcgt atttgtgaac ctcgatccgg
      901 atgctggttc gctgggcaat tttttgggga gcattccacc gattgtggct ccgctggaat
      961 tgacaaagaa gctgcactac tttgaccgcc gtatttacca tttgcagtgc aactggaaag
     1021 tgtatgtcga taactatctg gatggcggct accacgttcc gcacgcgcac aaaggactaa
     1081 gcagcgtgat cgag
//
LOCUS       DATCBB010000111         1192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_165680, whole genome shotgun sequence.
ACCESSION   DATCBB010000111 DATCBB010000000
VERSION     DATCBB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165680"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VII37_01300"
     CDS             <1..528
                     /locus_tag="VII37_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068381.1"
                     /translation="RKILVSFAMALAFPAVAICSPNGQQQSSKPADALALTGLPGVKD
                     NAKGKLTVEHGKLLFSYGKKSSDVNVAAIDDVVTGEDSQATVGKTVSTLSMAAPYGSG
                     RFLALFRTKFDTLTLRYRDENGALHGVIFALPAGGAEPIKKELLAQGAHTSVGEEQAA
                     TAPASANSSSKEQKQ"
     gene            564..1037
                     /locus_tag="VII37_01305"
     CDS             564..1037
                     /locus_tag="VII37_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HEY5068382.1"
                     /translation="MALAFAALPLSAQPVKTKTSAIQVEMIRSDEIKLPAEFQISLYE
                     NLVRQLEKQGGFAHVYRDGDRRAADAPELVVLHGTVRGFKQGSERARHVTTVAGATSI
                     TIHCEFKNTDGKLLLERDVNGKVWFFGGNLRATYDFAKKTARVAHDNFNFSVNKS"
     gene            complement(1001..>1192)
                     /locus_tag="VII37_01310"
     CDS             complement(1001..>1192)
                     /locus_tag="VII37_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068383.1"
                     /translation="NRWFFFVVHTGASPRCKCSIQLQIQAAAQGPGLPQAANLGHSTL
                     AARNAFGIYDLFTEKLKLS"
BASE COUNT          284 a          341 c          321 g          246 t
ORIGIN      
        1 agaaaaatcc ttgtttcgtt cgcgatggcc ctggcattcc ccgcagttgc gatttgcagc
       61 cctaacgggc agcaacagag ttccaagcca gccgatgctc tcgcacttac cggtctgccc
      121 ggcgtcaaag acaacgccaa aggcaagtta accgtcgagc atggaaaact gcttttcagc
      181 tacggcaaaa aaagttcgga cgtgaacgtg gccgccattg acgacgtggt caccggcgaa
      241 gactcgcagg ccacagtagg aaagacggtc agcaccctca gcatggccgc accatatgga
      301 tcgggccgct ttctggcctt gtttcgcacc aagtttgaca cgctgacgct tcgctatcgc
      361 gatgaaaatg gcgccttgca cggcgtcatc tttgcgctcc cggccggcgg cgccgagccc
      421 ataaagaagg agctgctggc gcaaggagcg catacctcgg tcggcgagga gcaagctgca
      481 acagcgccag cctccgccaa ttcatccagc aaggagcaaa aacaatgagg cggtattcga
      541 atgcaatttt gggcttagca gttgtggctc tggctttcgc ggcccttcct ttatcggctc
      601 agccagtcaa aaccaagacc tctgcgattc aggtggaaat gatccgctcc gatgagatca
      661 agctccctgc ggagttccag atttcgcttt atgaaaatct ggttcgccag ctcgagaagc
      721 aaggcggctt tgcgcatgtt taccgcgacg gcgatcgccg agctgcagac gctcccgagc
      781 tcgttgttct gcatggcacc gttcgcggtt ttaagcaagg cagcgaacgc gcccgccacg
      841 tgactactgt agccggcgcc acctccatca ccattcattg cgagttcaaa aatacggatg
      901 gaaaactgtt attggagaga gatgtcaatg gcaaggtctg gttcttcgga ggtaatttgc
      961 gagcgaccta cgacttcgcc aaaaaaaccg ctcgagtagc tcacgacaac ttcaacttct
     1021 ccgtaaacaa atcgtaaatt ccgaacgcgt ttcgcgccgc caatgtagag tggcccaggt
     1081 tcgcagcttg aggtaaacct gggccttgcg ctgcggcttg aatttgcaat tgaatcgagc
     1141 acttacaccg cggagatgcg ccggtatgca ccacgaaaaa aaaccagcgg tt
//
LOCUS       DATCBB010000112         1426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_360113, whole genome shotgun sequence.
ACCESSION   DATCBB010000112 DATCBB010000000
VERSION     DATCBB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360113"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VII37_01315"
     CDS             <1..209
                     /locus_tag="VII37_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068384.1"
                     /translation="IEAITDPDGDAWWNNLDVGLFGEEIGDECSFVLPPQYFFDPAII
                     RVQGHQYALQPEYDNSVHGCCAYQ"
     gene            complement(414..>1426)
                     /locus_tag="VII37_01320"
     CDS             complement(414..>1426)
                     /locus_tag="VII37_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY5068385.1"
                     /translation="MNWRSAQRGISVDRATALSSGSAWSTGSMWQSHSLLPFWSPRTH
                     VVGLDTLKAPPSGQTGGSMSGVIGGVPRNVRLAADYNSNSDATGETFERQVIRTGSLE
                     IIASDPLQTAEQLRNLAVSFSGFVVSSNVTGSDEGTRSAQVSIRIPSQYFDEVRAQVR
                     KIAKTVEQDTIEAHDVTRESVNQEAALRNARAEEVQYLSILKRAAAVRDVLEVSSRVA
                     EVRGRIDQLESGLRLLHHQVEMSLLAISIHGVAEAQAFGLHWRPLYEAKVSLRGALAG
                     MADYADDMVALFLNVPVIAIWVLTIVALIKIGWMLLRGTVRVFFPGSTIWLRRVVQPR
                     AT"
BASE COUNT          323 a          428 c          366 g          309 t
ORIGIN      
        1 tgatcgaggc catcaccgat cccgacggcg acgcttggtg gaacaattta gacgtcggtc
       61 tcttcggcga ggaaatcgga gacgagtgtt ccttcgtgct tcctccacaa tatttctttg
      121 accccgccat catcagggtg caagggcatc aatacgcttt gcagccggaa tacgacaaca
      181 gcgtccacgg ttgctgcgcc taccagtaaa aacaaaagag gcagcgagcc gagcagcttt
      241 cgacatttcc aaatggcgca attggcgcct gcgagatcgt cgggagccct tggccgccgc
      301 caatatccaa agtaggaaaa tgcaaatgca gtgcagccgt tgcatagtgg cggcaggatg
      361 atcggcgctt cgcttgcgcg cgtgtgacca aagcaaagtg gcagctgctt aacttatgtt
      421 gctctgggtt gaactacgcg ccgtagccat attgttgaac cgggaaaaaa cacgcgcacg
      481 gtgccccgta acaacatcca tccaatcttg atgagcgcga caatggtgag tacccatatc
      541 gctatgaccg gaacattcaa aaaaagcgcg accatatcgt ccgcgtagtc agccattccg
      601 gcgagtgcgc cccgcagcga tactttggcc tcatacagcg gccgccaatg caatccgaat
      661 gcttgcgcct cggccacacc atggatgcta atggcgagca aagacatttc cacttgatgg
      721 tgaagcaacc gcaggccgct ttccaactga tcgatgcggc ccctcacctc agctaccctg
      781 gagctgacct ccaagacatc tcttaccgcc gcggcgcgct tcagaatgga gaggtattgc
      841 acttcttcag ctcgcgcatt gcgcagggcc gcttcctgat tgacggattc tcgtgtcacg
      901 tcatgcgctt caatcgtgtc ttgctccaca gtcttcgcta tctttctcac ctgtgcccgc
      961 acctcgtcaa agtattggga cggaatgcgt atgctaacct gcgccgacct tgttccttcg
     1021 tcgctcccgg taacgttcga actcaccaca aatccggaaa aacttaccgc gagattgcgt
     1081 aactgttccg cggtttgcaa agggtcgcta gcaataattt cgagcgaccc agtgcgaatg
     1141 acttgccgct caaacgtttc tcctgttgca tctgagttgg aattgtagtc cgcagcaagg
     1201 cgaacatttc gaggcacccc accaatcacg ccgctcatcg aaccgccagt ctggccgctc
     1261 ggcggcgcct tcagggtatc tagaccaacg acatgtgttc tcggcgacca aaaaggcagc
     1321 agggaatgac tttgccacat cgaccccgtg ctccaggcgc tgcctgagga aagcgctgtt
     1381 gccctgtcta cagagatgcc gcgttgcgcg cttcgccagt tcataa
//
LOCUS       DATCBB010000113         1110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_457775, whole genome shotgun sequence.
ACCESSION   DATCBB010000113 DATCBB010000000
VERSION     DATCBB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457775"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VII37_01325"
     CDS             complement(<1..521)
                     /locus_tag="VII37_01325"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY5068386.1"
                     /translation="MARKSGSTISQEELYPHPDEPVREELDDARLLDLDAEQESPFLR
                     GQKRVSVRRGTIPKKTATGLAWTALAICVAFFCMAGVAALYHYGEHSWRFRIESSDDI
                     DVTGIHNVTRSQVMEIMGGDIGRNIFFIPLSRRKAQLEQIPWVGSASVMRFAPNRIAV
                     EIVERTPVAFARIG"
     gene            complement(640..>1110)
                     /locus_tag="VII37_01330"
     CDS             complement(640..>1110)
                     /locus_tag="VII37_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY5068387.1"
                     /translation="RFQLRGKVAGISVVDDYGHHPTEIRATLAAARQCKYGKIHVVFQ
                     PHRYTRTQLLMEDFANSFNDCDSLFLLDIYPASENPIDGVTSAVLARKIAAGGDLPAS
                     YISTFEDAIVSVAAIAEPGDMILTLGAGNVGQLAPMILEKLQIMKNSASAARSI"
BASE COUNT          259 a          295 c          303 g          253 t
ORIGIN      
        1 ccgatacggg caaatgcgac cggagtacgt tcaacgattt ctaccgcgat gcgattaggg
       61 gcaaatctca tcacactggc ggagcccacc catggaatct gctccagttg cgccttccgg
      121 cgcgatagcg ggatgaaaaa tatgttgcgg ccaatgtcgc cgcccataat ttccatgact
      181 tgtgaccggg tgacgttgtg gataccagtt acatcaatgt catcactgga ctcgatacga
      241 aatcgccatg agtgttcgcc ataatggtaa agagcagcga ctcccgccat gcaaaagaaa
      301 gcgacgcata ttgctagagc cgtccacgcc aaaccggtcg ctgttttctt tgggatcgta
      361 ccccggcgga cggagacacg cttttgtccg cgcaagaatg gcgattcctg ttccgcatcg
      421 aggtcaagca gccgcgcgtc atccaattct tcgcgcactg gctcgtcggg atggggatac
      481 aactcctcct gcgagatggt ggaaccgctt ttccttgcca tcaactcagg tcagttgtcg
      541 tcagcgaatc gcgagcaaaa ttactataac ggctttttgc cgactgacat agagaaagtt
      601 tggttcgctt tactcagaag gtcttgcgta ggggcgcggt tagatcgacc tggcggcgct
      661 cgcggaattc ttcattattt gaagtttctc caaaatcatg ggcgcgagct gtcccacgtt
      721 gccggcgccc agagtgagaa tcatgtcgcc tggttcggcg atggccgcga ccgagacgat
      781 ggcatcctca aaagtactga tataacttgc gggaaggtcg ccgccagcgg caattttgcg
      841 ggctaacacc gcgctggtga ctccatcaat gggattttcg ctggcagggt aaatatcgag
      901 cagaaaaagc gaatcgcaat cgttgaagga attagcgaag tcctccatca gcaattgagt
      961 tcgcgtgtaa cgatgcggct ggaacactac gtgaatcttt ccgtacttgc attgacgcgc
     1021 cgcagctagc gtggcgcgaa tttccgtcgg atgatgaccg taatcgtcca cgaccgagat
     1081 tccagcaact ttgccgcgca attgaaaacg
//
LOCUS       DATCBB010000114         1153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_101040, whole genome shotgun sequence.
ACCESSION   DATCBB010000114 DATCBB010000000
VERSION     DATCBB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101040"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..>1153
                     /locus_tag="VII37_01335"
     CDS             398..>1153
                     /locus_tag="VII37_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5068388.1"
                     /translation="MTTLATALLQGLKDHGAREIFGIPGDFVLPLYKVIEESKILPHF
                     TLSHEPAVGFAADAAARYHQGLGVAVVTYGAGAFNLVNAIAGAYAERSPVVVIAGAPG
                     ARERASGFLLHHQARSIDTQLAVYREITCDQAVLSDPASAPDAIARVLRSARELSLPV
                     YIEIPRDLIAAKTLAVPILPRRPADSGALAECADEILYKLAQARAPVMIIDVEIRRYG
                     IEEQTAKLARQLALPVVTTFMGRGLLEDASDVLA"
BASE COUNT          227 a          346 c          326 g          254 t
ORIGIN      
        1 atttcgaacc cgacctctga ccattccagc gcgcagaaga gtcaaccacg aaatggcgcg
       61 agagagattt tcgcgtccgc tgccggcgag cgcccggctt gcggcagcca ggattcactc
      121 tgctatccgt cctcaactca gcgcctcggc agatttgcta cgccctccgt tttgcgtcgt
      181 ttcaattttt tgaccaaacg ggatcgagga aacgtcggca tcattcgaag cccggtccgc
      241 gcctcgatcc tacccgacca ttccgggcat ccattttgtt ccaaaggacg tcaattttct
      301 gcgttctatc tttttgtttt gagagggggc tgcgggcgtt tttcccaaag cgcggcataa
      361 acatcccctc cgtttcataa cacgggtatt atgcggcatg accacgctcg cgacagcgct
      421 cttgcaaggc ctgaaggacc acggcgcgcg ggagattttt ggtatccccg gagacttcgt
      481 actgccactc tacaaggtta tcgaagagtc gaagattctt ccgcatttca cgctcagcca
      541 cgagccagcg gtcggcttcg cggcagatgc cgcggcgagg tatcaccaag ggctcggtgt
      601 tgccgttgtc acctatggtg ctggtgcctt caatctggtc aatgcaatcg ctggagctta
      661 tgctgagcgt tcgccagtgg tcgtgatagc cggggcgcct ggagcgcgag agcgcgccag
      721 cggcttcctt ctccaccatc aggcgcgaag catagatacc cagcttgcgg tctatcgcga
      781 gatcacctgc gatcaggcag tgctcagcga tccggcgagc gcacccgacg ctatcgcgcg
      841 tgtgttgcgt agtgcgcgcg agctatcgtt accggtctac atcgaaattc cgcgtgactt
      901 aatcgcggct aaaacgcttg cggtgccgat tttgccacga cggccagccg attcgggcgc
      961 gctggctgaa tgcgcggacg agatcctcta caaacttgcg caggcgcgcg cgccagtgat
     1021 gatcatagac gtcgaaatcc gtcgctacgg tattgaggaa cagacggcaa aactagcacg
     1081 ccaacttgca ttaccagtag tgaccacatt tatgggacgc ggcctgctcg aagacgcttc
     1141 ggacgtgctg gcc
//
LOCUS       DATCBB010000115         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_360153, whole genome shotgun sequence.
ACCESSION   DATCBB010000115 DATCBB010000000
VERSION     DATCBB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360153"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VII37_01340"
     CDS             <1..276
                     /locus_tag="VII37_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068389.1"
                     /translation="SVVRTLVEQTTAAAEIISAESILVSGGVAANSELRAVFEKRAAS
                     SGMEVFFPSRALSTDNAAMIAAAGYSRFRAGIVADAFLNPDPALVLK"
     gene            442..1344
                     /locus_tag="VII37_01345"
     CDS             442..1344
                     /locus_tag="VII37_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY5068390.1"
                     /translation="MAQSSSVRAAPAVLTSRDNRWLKQFRMALRGGIPTESGFVGVEG
                     VRLVEEALRSGCRIGAVLFSETGARYRERLAPFIGRPEIAFRVLTTTDRLFEGLADTE
                     HPQGVAALVEPRVTTFDDLVRAPEGVCSPLVVVLAGVQDPGNVGTILRTAAAFGATGA
                     AISASGQSGTASPFSPKALRASAGAALHLAVLSGTPLPILMTQLKVAGFRMLASSVHE
                     PETGEQPLLAPWEVNWCEASALLVGNEGSGLPVELERSADARIRIPMASGVESLNAAA
                     AAAVLFYEAARQRNSICTRNIQEP"
     gene            1341..2717
                     /locus_tag="VII37_01350"
     CDS             1341..2717
                     /locus_tag="VII37_01350"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY5068391.1"
                     /translation="MSLFNQMPLGMGGAGGNSLESEVPAAHQPLAERMRPRTLDEFIG
                     QEKLLGPGKPLRTQIENDDIGSMLLWGPPGCGKTTLARLIARLTSSEFVSFSAVLAGI
                     KEIKEVMAVAERKSRSGHRTIVFVDEVHRFNKAQQDAFLPYVEAGHITFIGATTENPS
                     FEVISPLLSRTKVYILAALPTPLIAELLRRALLDKERGLGNEEFEASDDVLMRIASFA
                     NGDARSAYNTLEPAARSAIRDRDGKLAITPLLLEDILQRKLLRYDKSGEEHFNLISAL
                     HKSVRNSDPDAALYWLTRMLESGEDALYLARRLVRMASEDIGLAEPGALAVTLAAKDA
                     FDFLGAPEGHLALAQAAVYLSLAPKSNALYTAHGEAMEDVAKTEADPVPLHLRNAVTG
                     LMKNVGYGAGYKYAHNFEEKVTDMTCLPENLAGRSYYKPTDQGFEGRLRQRLEEIRKI
                     KIKAATEK"
BASE COUNT          526 a          601 c         1004 g          598 t
ORIGIN      
        1 tcggtggtgc ggactttggt ggagcaaact actgctgccg ctgaaattat ctccgcggag
       61 tcgatactgg tgtcgggtgg agtggcggcg aacagcgagt tgcgtgcggt gtttgaaaaa
      121 cgcgcggcct cttctggcat ggaagtattt ttccctagcc gggcgctttc tactgacaat
      181 gccgcgatga ttgcggccgc ggggtattca cgcttccggg ctggaattgt ggccgacgct
      241 ttcctgaatc ccgatcccgc gctagttctc aaataacaac gaaaaacgag actgaaacgg
      301 ctctcaggtg attggtgaag tgcgtttggc gtgggaagga tgctacgctt ttgggcgatg
      361 agtacacctc caggagcatc gggagcgccg ccgggtgcga cgcgaaaagc gcggacgcca
      421 cgttcgtcaa gtgggccgac aatggcgcag tcttcttccg tgcgcgctgc gcctgcggtg
      481 cttaccagcc gtgataatcg gtggctgaaa caatttcgca tggcgctgcg tggaggaatt
      541 ccgacggagt cgggattcgt tggcgtggag ggcgtgcggc tggtggaaga ggcattgcgt
      601 tctggatgcc gcatcggtgc ggttttattt agcgagactg gcgcgagata tcgggagcga
      661 ctggcaccat ttattggacg gccggaaatt gcttttaggg ttttgacgac gacggaccgg
      721 ctgtttgaag ggctggcgga tacggagcac ccgcagggag tggcggcgct ggtagagccg
      781 cgcgttacta cgtttgatga tttggtgcgc gcgccggaag gagtttgttc gccactggtg
      841 gtggtgttgg ctggagtgca ggatcctgga aatgtgggga cgatcttgcg gacggcggcg
      901 gcctttggcg cgaccggggc ggcgatttct gcgtcgggac aaagtgggac ggcgagtcca
      961 ttttcaccaa aggcgttgcg ggcgtcggcg ggcgcggcgc tgcaccttgc agttttgtct
     1021 gggacaccgc ttccaatttt aatgacgcaa ttgaaggtgg cgggattccg aatgctggcg
     1081 tcaagtgtgc atgagcctga gacgggcgag cagccgctcc tggccccgtg ggaagtgaat
     1141 tggtgcgagg cttcggcgtt gctggttgga aatgaagggt cggggctgcc ggtggaattg
     1201 gaacgcagcg cggatgcgcg gattcgcata ccgatggcaa gtggcgtgga gtcgctaaac
     1261 gcggcggctg cggcggcggt gttgttttat gaagcggcgc ggcagcgcaa ctcgatctgc
     1321 acgcggaaca tccaagagcc gtgagcttat tcaaccagat gccgttgggt atgggcgggg
     1381 cgggaggaaa ttcgctggag tccgaggtgc ctgcggcgca ccagcctttg gcggagcgca
     1441 tgcggccgcg cacgctggat gaatttattg ggcaagagaa attgttaggg ccggggaaac
     1501 cgcttcgcac acaaattgaa aatgacgata ttgggtcgat gctgctgtgg ggtccgccgg
     1561 ggtgtgggaa gaccacgctg gcgcgattga ttgcgcgact gacgagttcg gaatttgtgt
     1621 cgttcagcgc ggtgctggcg gggattaaag aaattaagga agtgatggcg gttgcggagc
     1681 ggaagtccag atcggggcat cgcacgattg tgtttgtgga tgaggtgcac cgatttaata
     1741 aagcgcagca ggatgcgttt ctgccgtatg tggaggcggg acacattacg tttatcgggg
     1801 cgaccacgga gaatccttcg tttgaggtga tttcgccgct attgtcgcgg acgaaggtgt
     1861 acattctggc ggcgctgccg acgcctttga ttgcggaatt gctgcggcgg gcgctgctgg
     1921 ataaagagcg cgggttggga aatgaggagt ttgaagccag cgacgatgtg ttgatgcgga
     1981 ttgcttcgtt tgcgaatggc gatgcgcggt cggcgtacaa caccctggag ccggcggcac
     2041 gcagcgcgat acgagaccgt gatgggaagc tggcgattac gccgctgctg ctggaagata
     2101 ttttgcagcg taagttgctg cggtacgaca agagcggtga agagcatttt aatttaattt
     2161 ctgcgctgca taagtcggtg cgcaattcgg atccggatgc ggcgctgtat tggctgacgc
     2221 gcatgctgga atcgggtgag gatgcgttgt acctggcgcg gcggctggtg cgcatggcta
     2281 gcgaggatat tggattggcg gagcctgggg cgctggcggt gacgctggcg gcgaaagatg
     2341 cgtttgattt tttgggcgcg ccggaggggc acctggcttt ggctcaggcg gcggtttatc
     2401 tgtcgttggc gccgaagtcg aatgcactgt ataccgcgca tggcgaggct atggaggatg
     2461 tggcgaagac ggaagcggat cctgtgccac tgcatttgcg aaacgcggtg acgggactta
     2521 tgaaaaatgt ggggtacggg gctggataca agtacgcgca caatttcgag gaaaaagtta
     2581 cggacatgac ttgcttgccg gagaatttgg cggggcggag ttattacaag ccgacggatc
     2641 agggatttga ggggcgattg cggcaacggt tagaggagat tcggaaaatt aaaataaagg
     2701 cggcgacgga gaagtgatac ggcttcgcg
//
LOCUS       DATCBB010000116         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_133353, whole genome shotgun sequence.
ACCESSION   DATCBB010000116 DATCBB010000000
VERSION     DATCBB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133353"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VII37_01355"
     CDS             complement(<1..416)
                     /locus_tag="VII37_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5068392.1"
                     /translation="MNPFLHLEPLSADESTGSLLEALGPTEERRPQSQYCVFRSGRER
                     LCLPVLDVEEVLDWPVLTKVPLSPAYLVGIFNLRGTIVPLIDIAMTEGRRPGLLPRHV
                     VVASLAADGHHEILRVGIAADEVIGTYSVNSDEVLE"
     gene            complement(446..808)
                     /locus_tag="VII37_01360"
     CDS             complement(446..808)
                     /locus_tag="VII37_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5068393.1"
                     /translation="MQKKVLIVDDSPAEVRLMHGVLEREGYRLVDLHDSMRVEETIAF
                     EQPNVILLDVVMPGKNGFQVCRELKGHDQYKTIPVILVTSRDTASDKFWGEQQGADGY
                     VTKPFTSEELLRAVKRFA"
     gene            complement(940..1650)
                     /locus_tag="VII37_01365"
     CDS             complement(940..1650)
                     /locus_tag="VII37_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068394.1"
                     /translation="MNTSGMDCPLPNASVLILTDETEFATIAAGCWQAQRKSLDITVL
                     NSKLWRNYDATAHDLIVVGPISGRKHHEVLRSLDRQSAVILCIPADAREADELRARYP
                     RVLHVQQREDWSHTLLLLAAETLRRVEALGLAKQAEREAAKYQRLATLGRYMTDMKHS
                     VNNALTSMLGNAELLLLEPGQLSSQSLAQIRTIHSMAMRMNEIMLRFSSLANEMREAE
                     NASQAETEVVDSPTPSPR"
     gene            complement(1681..>2022)
                     /locus_tag="VII37_01370"
     CDS             complement(1681..>2022)
                     /locus_tag="VII37_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068395.1"
                     /translation="LHGRSMSTVKLGDTRILLSSWEVAAFVSETGVAAEDLRRAVAAR
                     AMVSTATTNAKEKGHTFALDKALAVGRVEASRLQDRVDAAKQAKDTEAAVNLGISAKR
                     LLSALDEAEKL"
BASE COUNT          446 a          577 c          552 g          447 t
ORIGIN      
        1 tgttccaaca cctcgtcgct gtttaccgaa taagtcccga ttacttcatc agctgctatt
       61 cccacacgaa gtatttcgtg gtggccgtcc gcggccaacg aagccaccac tacgtgacgc
      121 ggcagcaggc cggggcggcg accttcggtc atggcaatgt cgatcaaagg cacgatcgtg
      181 ccacgcaaat taaaaatgcc gactaaatag gcggggctca gcggtacctt cgtgagaacc
      241 ggccaatcca atacttcctc cacgtccaga accggaaggc ataaccgttc acggccgctt
      301 cgaaacacgc agtattgcga ttgcggccga cgctcttccg tgggaccgag agcttccagc
      361 aaactgccgg tgctctcgtc ggccgatagc ggttcgaggt gaagaaatgg attcatcgcg
      421 atttagtaaa tctccacaaa aattttcatg cgaaccgctt caccgctcgt agcaattcct
      481 cagacgtaaa aggtttggtg acataaccgt cggcgccttg ctgctcaccc caaaatttgt
      541 cgctggcggt gtcgcgggaa gtgaccaaaa tgactgggat cgttttgtat tggtcgtggc
      601 cttttaattc acggcaaacc tgaaacccgt ttttgccggg catgaccacg tccagcaaaa
      661 tcacgttggg ttgctcgaag gcgatggttt cctctacgcg catggagtca tgcagatcaa
      721 caagccggta accttcgcgt tccaataccc cgtgcatcaa ccggacttcc gccggtgaat
      781 cgtcgacgat gagcactttt ttctgcacgg ctaaaatccc tcggactaat ccaggaaaca
      841 aaggtgcacg ccataggcca gctttgcgga ccgctaagag aatgttacgt attaggtaaa
      901 ggcccgataa aacaaggact tagaacgagg tctacggctc tatctaggag acggcgtggg
      961 gctgtcaact acttctgtct cagcttgaga agcattctca gcctcgcgca tctcgttggc
     1021 cagcgatgag aatcgcagca tgatttcgtt catgcgcata gccatgctgt ggatggttcg
     1081 gatctgcgcc agcgattgcg acgaaagctg tccgggttcc agcaataaca attcggcatt
     1141 ccccagcatt gaggtcagtg cattattcac gctgtgcttc atatccgtca tgtaacgtcc
     1201 gagcgtggcc agtcgttgat acttggcggc ttcgcgttcc gcctgtttcg ccaagcccag
     1261 ggcttcaaca cggcgcagtg tctcggcagc cagcaacaat aaggtgtgtg accagtcttc
     1321 ccgctgctgc acgtgaagga ctcgcgggta gcgagcgcgc aattcgtctg cttcgcgagc
     1381 gtccgcgggg atgcaaagaa tgaccgcgga ctggcggtcg agcgaacgaa gaacctcatg
     1441 atgtttccgg ccgctgatgg ggccgacgac tatgaggtcg tgcgccgtcg cgtcgtagtt
     1501 acgccagagc ttgctgttca aaacggtaat gtcgagtgat ttgcgttggg cctgccaaca
     1561 tccggccgca attgttgcga attcggtttc atcagtcaga atcaggacgc tggcgttcgg
     1621 caaaggacaa tccatcccag aagtattcac ccgcaatggc cggggaaatt atgttgcacg
     1681 ctacaatttt tctgcttcat ccagtgcgga cagcaggcgc ttggcgctga tccccagatt
     1741 gacggccgcc tcggtatctt tcgcttgctt ggcagcgtcc actcgatcct ggagcctcga
     1801 agcttccacg cgtccaaccg cgagggcctt gtccagggca aaagtgtggc ctttctcttt
     1861 cgcattagtg gtggcagtgg aaaccatggc gcgcgcggcc acagcgcggc gcaaatcttc
     1921 cgcagcaacc ccggtctcac tcacgaacgc ggccacttcc caggaggaca acagaatgcg
     1981 cgtatcgcca agcttcaccg tggacatgga gcgcccgtgc aa
//
LOCUS       DATCBB010000117         1711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_295899, whole genome shotgun sequence.
ACCESSION   DATCBB010000117 DATCBB010000000
VERSION     DATCBB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295899"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VII37_01375"
     CDS             complement(<1..747)
                     /locus_tag="VII37_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5068396.1"
                     /translation="MAVLQNLEVREKKRAALLSVGSAVVLLSLKTFLVVRTGSLGVLS
                     EALHSGLDFLAAIITYLSVRMSDQPADEGHPYGHGKFENFSAFVETALLLITAVFIIY
                     EGIDRLFFHHVHIQPSVIAVLILLVALSIDITRAKALGHVARKYHSEAIEADALHFST
                     DVWSTIVVIAGIGLVWAGETWHLPWLVYADAIAGLAVAGVILWVGSQLGRRTLDALVD
                     AAPEGLRQEIARAVGRMEGVLDVDRVRVRRA"
     gene            complement(844..1428)
                     /gene="msrB"
                     /locus_tag="VII37_01380"
     CDS             complement(844..1428)
                     /gene="msrB"
                     /locus_tag="VII37_01380"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY5068397.1"
                     /translation="MKEFTRRGFVYAAATLAGYGVLIGVERVCSSAFADGVEPTSGPG
                     PVKIIKVADDGTSMGALTTPKVQKSTAQWKAQLTALQFDVTRRAATEWAFTGALDNEH
                     RAGLYRCIDCDNALFDSRAKFDSGTGWPSFFEPIAKENVREQKDISYGVVRTEVKCTL
                     CEAHLGHVFTDGPRPTGLRYCMNSAALRFIPRKG"
     gene            complement(1477..>1711)
                     /locus_tag="VII37_01385"
     CDS             complement(1477..>1711)
                     /locus_tag="VII37_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068398.1"
                     /translation="IRNIAGIGNDNYVYNYMQSQINGFAGTALGAFGSAQNGLPASIV
                     APSLLFEEVEVRGARGEPKRLPLVAPPPMTASK"
BASE COUNT          405 a          497 c          472 g          337 t
ORIGIN      
        1 agcgcggcgg acgcgtacgc gatcgacgtc caagacgcct tccatgcgac ccacggcccg
       61 agcgatctct tggcgaaggc cttctggcgc ggcatccacg agagcgtcga gagtgcgcct
      121 gccaagctgc gatccgaccc acaggatgac gcctgccacg gccaaaccag caatggcatc
      181 ggcatacacc agccagggca gatgccaggt ttcgccggcc cacaccagcc ctatgcccgc
      241 gatgactacg atggtgctcc agacatccgt cgaaaaatgc agcgcgtccg cttcgattgc
      301 ttcgctatgg tatttccggg ctacatgtcc cagcgctttc gcgcgggtta tatcgatcga
      361 taaagctacc aacaggatca gcacagcaat aacgctcggc tgaatatgaa catgatggaa
      421 gaacagccgg tcgatgcctt cgtagatgat gaacacggcg gtaataagca gcaacgcggt
      481 ttccacgaag gccgaaaaat tttcaaactt accgtggccg tacgggtgac cctcatcggc
      541 aggctgatcc gacatgcgca cggaaagata agtgataatc gcggccagaa aatcgaggcc
      601 agaatgcagc gcttcggaca acacgccaag gctaccagtg cgaaccacca agaaagtttt
      661 aagcgacagc aacaccaccg ccgagcctac gcttaacagc gcggcgcgct tcttttcccg
      721 tacttccagg ttttgcagga cagccatcag gtttgcctag actatcctaa cttcaagcgg
      781 tttatgggca gagcttgcgc aatgcgtgct ttggaaggtg aggaaacgct aagaaccgag
      841 gcgctaacct ttgcgcggga taaatcgcaa tgcggccgaa ttcatgcaat aacgcagccc
      901 tgttggccgc ggtccatcgg taaaaacgtg accaagatgg gcctcgcaga gagtgcactt
      961 tacctccgtg cgcactaccc cgtaggaaat atctttttgt tcacggacgt tttccttggc
     1021 gataggctcg aaaaaactgg gccatccggt gccagagtca aacttggcac gcgaatcaaa
     1081 tagcgcgtta tcgcaatcga tacagcgata aaggcctgcg cggtgttcgt tatccagcgc
     1141 gcctgtgaaa gcccattccg tggccgcgcg ccgggtgacg tcaaattgca aagcggtaag
     1201 ttgggctttc cactgcgcgg tgctcttttg tactttgggg gtcgtcaagg cgcccatgga
     1261 agtgccgtcg tccgcaactt ttataatttt caccggcccg ggaccgctag ttggttcaac
     1321 gccatcggca aaagcggagg aacaaacccg ctcgacgcct atgagcacac catagccggc
     1381 tagcgtagcc gctgcgtaca cgaaaccgcg tctcgtaaat tctttcatgg gtgctgactc
     1441 aaaaactccg acaaggcggc gaaatcacca ccacacttac ttcgatgcgg tcatgggcgg
     1501 aggagctacg agcggcaagc gcttgggctc gccgcgggct ccacgcactt ccacttcttc
     1561 aaagaggagc gatggcgcga caatcgatgc cggcaaaccg ttttgcgcgg agccaaaagc
     1621 gccgagcgcg gtgcctgcga atccgtttat ctggctctgc atgtagttgt agacataatt
     1681 gtcgttgccg atgcccgcga tattgcgaat c
//
LOCUS       DATCBB010000118         1601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_3614, whole genome shotgun sequence.
ACCESSION   DATCBB010000118 DATCBB010000000
VERSION     DATCBB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VII37_01390"
     CDS             <1..1243
                     /locus_tag="VII37_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068399.1"
                     /translation="STVWLHMGAFGPKRVVTLDDSHTPAAPYSIVNNEYSLLDVSDLV
                     FVDAPGTGFSRIIGKDREKAFYGVDQDAQAFADFITQFLGKYGRWNSPKYMFGESYGT
                     TRAAVLANVLERERDVDFNGVILLSQILNFNFSADEPEFNAGVDLPYQLVLPTYAASA
                     WYHHKLPGAHQELAALLSEVEHFSMSDYAQALGAGATLAPEQRRAIVSKLHEYTGLPA
                     EYIEKANLRISAGEFEKTLQDDSDTTTGRLDTRFSGPTLDPLSKEAEYDPQSAAISSA
                     YVSAFNDYVRKELKFGENRTFKPMIGIWRTWNFLHQAPGASLPLPQATNVMTDLALAM
                     KYNPELKVMLNAGYFDLATPFYEGVYEMQHLPIPAKLQKNIEYQFYESGHMVYAHEAS
                     LKQLHDNVAAFIAKTQTSKSK"
     gene            1340..>1601
                     /locus_tag="VII37_01395"
     CDS             1340..>1601
                     /locus_tag="VII37_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068400.1"
                     /translation="MDKLASDFWAWRATYRPFTGDDVPRMEHSGGVRDWSAAALAKQR
                     VDLGEFERRWKDLHTAGWPVARMVDYRLMGSAFARVRWELDVN"
BASE COUNT          356 a          405 c          492 g          348 t
ORIGIN      
        1 atcgacggtg tggctgcaca tgggggcgtt tgggccgaaa cgcgtagtta cgttggatga
       61 ttcgcacacg ccggctgcgc cgtattcgat cgtcaataac gagtacagcc tcttggatgt
      121 gagtgacctg gtatttgtgg atgcacccgg aacgggattc agccgcatca tcggaaaaga
      181 tcgggaaaaa gctttctacg gcgtcgatca ggatgcgcag gccttcgccg atttcatcac
      241 gcaattcctg gggaagtatg gccgctggaa ttcgccaaaa tatatgttcg gggaaagcta
      301 cggcaccact cgagctgcag tgctggcgaa cgtgcttgag agagaacggg acgtggactt
      361 caacggcgtg attttgttgt cgcaaattct aaatttcaat ttcagcgctg acgagccaga
      421 gttcaatgcc ggcgtcgatt taccctatca actggttctg cccacctatg ccgcatccgc
      481 gtggtatcac cataaactgc cgggcgccca tcaggaattg gcggcgttgc tgtcagaggt
      541 ggagcacttt tcaatgagcg attacgcgca ggcactgggg gcgggagcta cgttggcgcc
      601 cgagcagcga cgcgcgattg tctcaaagct ccatgagtac acggggttgc cggccgaata
      661 tatcgagaag gcgaatctgc gaatcagcgc aggagaattc gagaaaacgt tgcaggacga
      721 cagcgatacg acgaccggcc gcctggatac gcgtttttcg gggcccacgt tagatccgct
      781 cagcaaagag gcggagtacg atccgcaatc ggcagcgatc agctcggcgt atgtttcagc
      841 gttcaacgac tacgtgcgta aggaactgaa gtttggcgaa aatagaacgt tcaagcccat
      901 gattggaatc tggaggactt ggaacttttt gcatcaggcc cccggggcct cgctcccgct
      961 gccgcaagct accaacgtca tgacggacct ggcgcttgca atgaagtaca acccggagct
     1021 caaagtgatg ttgaatgcgg ggtatttcga tctggcgacg ccgttttacg aaggcgtgta
     1081 tgaaatgcag catctgccga ttcccgccaa attgcagaag aacatcgaat atcagtttta
     1141 cgaatcgggg cacatggtct atgcgcatga ggcttcactg aagcagctgc atgacaatgt
     1201 ggcggcgttt atcgccaaga cgcagacttc caagagcaag taaaaccagc ggggaactga
     1261 gggcgctcct atgtggcggg tgtttgtcgc attgttatta ctgtcttccg tctcgtcagc
     1321 accagcgaga gcggattcgt tggacaagtt ggcgtcggat ttctgggcct ggcgcgcgac
     1381 ttatcgcccg tttacaggtg acgacgttcc gcgcatggag cattccggtg gagtgcgcga
     1441 ttggtctgcc gcagcgctag ccaaacagcg cgtggatctt ggcgaattcg aacggcggtg
     1501 gaaagacctg cacaccgccg gctggccggt cgcgcggatg gttgactacc ggctgatggg
     1561 ctcggccttt gcgcgggtac gctgggaatt ggatgtaaat c
//
LOCUS       DATCBB010000119         1338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_425582, whole genome shotgun sequence.
ACCESSION   DATCBB010000119 DATCBB010000000
VERSION     DATCBB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425582"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VII37_01400"
     CDS             complement(<1..665)
                     /locus_tag="VII37_01400"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5068401.1"
                     /translation="MARAPKNPETLNMRPVWADVSLSALIHNFESIRSYVNPRNEKRR
                     TPRQVLCIVKGNAYGHGGPPVTKALEKAGADWFGVTCTEEGIALREAGGRKPILILTS
                     FVPGEEKRLLEHNLTAVIHRCEQLSQLEAAAARRGKKKVLFHLKIDSGMNRLGIATSS
                     VDCFARQLTKSRHLQLSGVMTHFASSEAFTDTVAGRQTREQEERFFAALERLRALGID
                     PGI"
     gene            complement(971..>1338)
                     /locus_tag="VII37_01405"
     CDS             complement(971..>1338)
                     /locus_tag="VII37_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5068402.1"
                     /translation="RNEEVASISRALKGLAKELQIPVLVLSQLTRAPERDDRGPQLSD
                     LRESGAIEQDADVVMFIYRPHFFKQGATPEEREETELKIAKQRNGPTESVKFVFRSRL
                     TRFEEAAPDAFSQFAADDM"
BASE COUNT          289 a          424 c          335 g          290 t
ORIGIN      
        1 ataatgcccg gatcgattcc cagcgcgcgt aatcgctcca gcgccgcaaa aaaccgttct
       61 tcttgctcgc gtgtctgccg tcccgccacc gtatcggtaa atgcttccga ggacgcaaaa
      121 tgcgtcatca cgccgctcaa ctgcaggtgc cgcgatttcg tgagttgccg cgcaaagcaa
      181 tccacactcg acgtcgcgat gcccagccga ttcatccccg aatcgatttt cagatgaaac
      241 aaaacctttt tcttcccgcg tcgtgccgcg gcagcttcga gttgtgacaa ttgctcgcag
      301 cggtgaatca ccgccgtcaa attgtgttcc agcaatcgct tttcttcacc gggcacaaag
      361 ctggtcagga tcaggattgg cttgcgccca ccagcctcgc gtagcgcaat gccttcttcc
      421 gtgcaagtca cgccaaacca gtccgccccg gctttctcca gcgctttggt gaccggcggg
      481 ccgccatggc catacgcgtt gcccttcacg atgcacagca cttgccgcgg cgtacgccgc
      541 ttttcatttc gcggattcac gtaggagcga atcgattcaa aattgtgaat gagcgctgag
      601 agcgaaacat ccgcccaaac gggccgcata ttcagcgttt cgggattttt cggtgctcgc
      661 gccatggtta ttccaggaaa aataagttta cgtcatccac gcggcagggg tggttttacg
      721 cctgaaaact gccgcgtgaa tgacaacagc gggcaactgc acccacgtac aggcacgaca
      781 cgtaccgaag gttgtgacaa gccgaaggca ggcagaaatt tagctaacgc tatatgcggg
      841 aaggacttgc tacttcagac cacgaaggat tgctcgggca ctcggatcgc aaccggtccg
      901 ctcgcgtggc ccacttgcca cgccgtgtca ctagccacat aacgccggct aaaccacgcc
      961 acgctaacct ctacatatca tcagcagcga attgtgaaaa cgcatctgga gccgcttctt
     1021 caaagcgcgt caaccggctg cggaatacaa atttgacgct ttccgtcggt ccatttcgct
     1081 gcttggctat cttgagctcg gtctcttccc gttcttccgg cgtagctccc tgcttaaaaa
     1141 aatgtggtcg ataaatgaac atgaccacgt ccgcgtcctg ctcaatggcg cccgactcac
     1201 gcaaatccga aagctgcggc ccgcggtcat cgcgttccgg cgcgcgtgtc aactggctaa
     1261 gcaccagcac cgggatctgc aactctttcg ccaggccctt cagcgcccgc gaaatactgg
     1321 ccacttcctc attgcgat
//
LOCUS       DATCBB010000120         4434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_263480, whole genome shotgun sequence.
ACCESSION   DATCBB010000120 DATCBB010000000
VERSION     DATCBB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263480"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VII37_01410"
     CDS             <1..603
                     /locus_tag="VII37_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HEY5068403.1"
                     /translation="ASRDRRPTFLEIIRKVAAELAIPLTAGGGIRTLDDARAVVGAGA
                     DKVTVNTAAVLRPELISELSEEFGSQAVVLAIDAKRDGESWRVMTTGGRVSTELDAVS
                     WATKGAELGAGEILLTSVDRDGTQSGFDVALTAAISKQVMVPVIASGGARWPQHFAEI
                     FGEGAADAALAASIFHDGIQSIRSLKEFLAGEGIRVRLPC"
     gene            597..1286
                     /locus_tag="VII37_01415"
     CDS             597..1286
                     /locus_tag="VII37_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY5068404.1"
                     /translation="MLIPCIDLQNGQAVQLVHGRKRELAISDVLGVLERFKNYEWLHV
                     IDLDAAMGKTSNAKLVRQLCFTARTTYGMKVRVGGGIRTVARAVKVAGWKPEQIIIGS
                     AVFRDGKVNLAFLERLAKQVEPARIVIALDTAKGRITTQGWRRTVKHRAEDVMAQLQP
                     YCAAFLCTDVDREGTMNGANLKWFRSLRDATDQPVIAAGGIKARREVSALEKIGMDAA
                     VGMAMYKNMLR"
     gene            complement(1376..3148)
                     /gene="glgP"
                     /locus_tag="VII37_01420"
     CDS             complement(1376..3148)
                     /gene="glgP"
                     /locus_tag="VII37_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY5068405.1"
                     /translation="MECIKDRLVAYFSMEIALENSMPSYSGGLGVLAGDTIRAAADLR
                     LPMVAVSLLYRKGYFSQHLAEDGTQTEEPVEWRVEDFLHEESPRVTIPLEDRRVELRC
                     WRYDAVGVRGYVVPIYFLDADLPSNAPADRALTGTLYGGDPYYRLCQEVILGIGGVRM
                     LRALGHDGLTRYHMNEGHAALLSLELLEAEAAKAGRTTIRAEDIEKVRCKCVFTTHTP
                     VPAGHDRFPMEYLTRAFPSQTELFDMKDASAADLVKRVLQAEQAFPDLQQAARAGASL
                     NMTYLALSLSKYVNGVAKQHGETSRQMFPGVPIEAITNGVHVGTWTAPSFREIFDRYI
                     PTWREDNYSLRGALGLPPEEIWTAHLIAKHELLEVVREKTGIKFDPGKFTIGFARRAT
                     GYKRADLILADLDRLRQIAKNSGPFQIVYAGKAHPHDAGGKSIIKHIFQAKKALRKAV
                     PVVFLDDYNMDLGGKMTAGVDLWLNTPQFPLEASGTSGMKAALNGVPSLSILDGWWSE
                     GHIEGVTGWSIGEVRRATPDLVTDNRADAESLYSKLESIIIPMFYNERYRYLSVMQHA
                     IAINGSFFNTQRMVQQYITDAYMR"
     gene            complement(3329..>4434)
                     /locus_tag="VII37_01425"
     CDS             complement(3329..>4434)
                     /locus_tag="VII37_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5068406.1"
                     /translation="ADVIKVERPGIGDQARGWGPPFVGELSAYFLSTNRNKRSIVLDY
                     NQANGLEVLHKLIANADIFITNQPSLESLRKRQLDAPTLCEHHPKLIYCGISGYGFSG
                     PKAGSPGYDILAQAEAGLMSFTGDENSGPMRYPIAIADMTCGIYAAMGILAALFAREK
                     TGQGQYLDMALFDSQLTWLANAGSSFLNAGVTPRRWGNAHPNIVPYEMFSGSDGTYFV
                     IGVGTEALWQNFTGIINTTIDLRRDPRFQNNAERIKHRLELIPILQEGFNKLSAAEWL
                     ARFATAGIPAAKINGASEALNDIQAKARGLVVQLEHPMLGIVRTIANPIRLSDTPVTY
                     RFAPPFLGEHTKEILEALGYSELPTQGLTGSPS"
BASE COUNT          976 a         1039 c         1396 g         1023 t
ORIGIN      
        1 gcatcgcggg atcggcggcc tacatttctg gaaataattc gcaaggtggc ggccgaattg
       61 gctatcccgc tcacggcggg cgggggcatt cgaacgttgg acgacgcgcg cgcggtggta
      121 ggggccgggg cggacaaagt caccgtgaac actgccgcgg tgttgcggcc tgaattgatt
      181 tcagaattgt cggaggaatt tgggtcgcag gcggtggttt tggcgattga tgcaaaacgg
      241 gatggcgaga gctggcgcgt aatgacgaca ggcgggcggg tgtccacgga attggatgct
      301 gtttcgtggg ctacgaaggg ggcggagctt ggagctggag aaattcttct gacctccgtg
      361 gaccgcgacg gaacacaaag tgggttcgat gttgctctca ctgcggcgat ctcgaaacaa
      421 gttatggtgc cggtgattgc ttccggcggg gccagatggc cacaacattt tgcagagatt
      481 tttggcgagg gcgcggcgga tgcggcgttg gcggcttcca tttttcatga cgggattcaa
      541 agcatacgtt cgctcaagga gttcctggcc ggcgagggca ttcgggttcg gctgccatgc
      601 tgattccttg catcgaccta caaaatggac aggcggtgca gttggttcat gggcgcaaac
      661 gcgaactggc gatttctgat gtacttggag tgctggagag atttaaaaac tacgagtggc
      721 tgcatgtcat cgatctggac gcggcgatgg gtaaaacttc caacgcaaag ctggttcggc
      781 aattgtgttt taccgcgcgg acgacttacg ggatgaaagt tcgggtcggt ggaggaatcc
      841 gcacggtggc gcgcgcggtg aaggtcgcgg ggtggaagcc ggaacaaatc atcattggga
      901 gcgcggtgtt ccgtgatgga aaagtgaatc tagcgtttct ggagcggttg gcaaagcaag
      961 ttgagcctgc gcggattgta atcgccttgg atacagctaa aggtcgcata acgactcagg
     1021 gttggcggcg cacggtcaaa catcgcgcgg aagatgttat ggctcagttg cagccatatt
     1081 gtgcggcgtt tctttgcacg gatgtggatc gggaaggaac tatgaacgga gccaatttga
     1141 aatggtttcg gagtttgcgt gacgctacgg atcaaccggt gattgccgcg ggcggaatta
     1201 aggccaggcg tgaagtgtcg gcgttagaaa aaataggtat ggacgcggcg gtgggaatgg
     1261 cgatgtataa aaatatgctg cgctagaaaa aaacacggcg cggatccgaa tttatttagc
     1321 atggaatttt cggtcaacgg aacagcaatt tgcctaaggt tcgttgggaa aaaaactagc
     1381 gcatgtatgc gtcggtaatg tattgctgca ccatgcgctg cgtgttgaag aaagatccgt
     1441 tgatggcgat ggcgtgttgc atgacgctga gataacggta gcgctcgttg taaaacatcg
     1501 gaattattat gctttctaac ttggagtaaa gtgattcggc gtcggctctg ttatcggtga
     1561 ccaaatccgg cgtggcgcga cgaacttccc cgatggacca accggttacg ccttcgatgt
     1621 gaccttcgga ccaccagcca tccagaatgc tgaggctggg cacgccgttt agagcggcct
     1681 tcatgccgct ggttccggaa gcttccagag gaaattgcgg agtgttgagc cagagatcca
     1741 cgccagcggt catctttcca cccaaatcca tgttgtagtc atcgaggaag acgaccggca
     1801 cggctttgcg caatgctttt ttggcctgga aaatatgctt gatgatggat tttccgccgg
     1861 cgtcgtgggg atgagctttg ccggcgtaga caatttgaaa tggtccggaa ttttttgcga
     1921 tctgacgcag gcgatccagg tcggcgagaa tcaaatcggc gcgcttataa ccggtggcgc
     1981 gacgggcaaa tccgatagtg aattttccgg ggtcaaactt gatgccggtt ttctcgcgca
     2041 caacttcaag gagctcatgc ttggcgatga gatgagcagt ccagatttct tccggcggaa
     2101 gacccagcgc gccacggagg ctgtagttgt cttcgcgcca ggtgggaatg tagcgatcga
     2161 aaatttcgcg gaacgacgga gcggtccagg tgcctacgtg gactccgttg gtgattgctt
     2221 cgatgggtac gccggggaac atttggcgcg aggtttcgcc atgttgcttg gcgacaccgt
     2281 tgacgtattt gctgaggcta agtgcgagat acgtcatgtt tagggaggca ccggcgcgcg
     2341 cggcttgctg caagtctgga aaggcttgtt cggcctgaag cacgcgcttc acgaggtccg
     2401 ctgcggaagc atctttcata tcaaaaagct cggtctgact gggaaatgcg cgagtcaggt
     2461 actccattgg aaatcgatca tgaccggcgg ggacgggcgt gtgggtggtg aaaacacatt
     2521 tgcagcgaac tttttcaatg tcctcggcgc gaatggtggt gcgtccggct ttggcggcct
     2581 cggcttcaag aagttccagg gagagaaggg cggcgtgacc ttcgttcatg tggtagcggg
     2641 taagtccatc gtggcctaaa gctcgaagca tgcgaacgcc accaatgcca agaatgactt
     2701 cctgacaaag acggtagtag ggatcgccgc cgtagagagt tccggtgagc gcgcgatctg
     2761 caggcgcgtt ggaaggcaaa tcggcatcca gaaaataaat cgggacgaca tatccgcgca
     2821 cgccgacagc gtcataacgc caacagcgaa gttccacgcg gcggtcttca agcgggatgg
     2881 ttacgcgcgg tgattcttcg tgcagaaagt cctcgacgcg ccattcgacg ggttcttcgg
     2941 tttgggttcc gtcttcggcg aggtgctgag aaaaatatcc tttacggtaa agcagcgaaa
     3001 ccgcgaccat cggcaagcga agatcagcag cggcgcgaat ggtgtctcca gcaaggacac
     3061 ctagtccgcc gctgtagctg ggcatggaat tctcaagcgc aatctccatg gagaaatacg
     3121 cgaccaatcg atctttaatg cactccattc ggttccttct tcactattcc ttcttccccg
     3181 ttgggggatt aacgattatt tatgtagggg gagcttgcgt catcgacgct actcattgtc
     3241 ggggaatccg tgcgagcagg caagcggttg cgggataaat tcgcgcgggt gttgcatgga
     3301 acactgtccc cgagttaagt aattctaatt aactcggtga acctgttagc ccttgcgttg
     3361 gcagttcgga atagccaaga gcttcaagga tctctttggt gtgctcgccc aaaaagggcg
     3421 gggcgaagcg gtaggttacg ggagtgtcag ataaacggat gggattggcg atggtccgaa
     3481 caattccaag catggggtgt tccagctgca cgacgaggcc gcgtgccttg gcctgaatat
     3541 cgttgagggc ttccgatgca ccattgattt tcgcggcggg aatgccggcg gttgcaaaac
     3601 gcgctagcca ttctgcggct gataatttgt tgaacccttc ctggaggatg ggaatcagtt
     3661 ccagtctgtg tttgatgcgc tcggcattat tttgaaatct tgggtcgcgg cgcagatcga
     3721 tagtggtatt gatgatgccg gtgaagtttt gccacagagc ttcggtgccc actccgataa
     3781 caaaataagt gccgtcgctg ccactgaaca tctcgtacgg gacgatgttc gggtgtgcat
     3841 ttccccaccg gcgtggtgtc acgccggcgt tcaagaaact gctgccggca ttggccagcc
     3901 aggtcagttg agaatcaaaa agtgccatat ccagatactg gccttgcccc gttttctcgc
     3961 gggcaaaaag cgcagccaga attcccatgg cggcgtaaat gccgcaggtc atatcggcga
     4021 tggcgatggg ataacgcatc ggtccagaat tttcatcacc ggtgaaactc atgaggcctg
     4081 cttctgcctg ggcgaggatg tcataccccg gtgagccggc tttgggaccc gaaaaaccgt
     4141 atccgctgat gccgcaataa atcagtttgg gatgatgctc acacagagtt ggagcgtcga
     4201 gctgtcgttt tcgcaatgat tccaaggatg gctgattggt gatgaaaata tccgcgttag
     4261 caattagttt gtgaagcact tcgaggccat tcgcttggtt gtaatcgagc acgatggaac
     4321 gcttgttgcg attggtagac aaaaaatagg ccgacagttc gcctacgaat ggcgggcccc
     4381 atccgcgggc ctgatcgccg atgcctggac gctccacttt aattacatca gctc
//
LOCUS       DATCBB010000121         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_457962, whole genome shotgun sequence.
ACCESSION   DATCBB010000121 DATCBB010000000
VERSION     DATCBB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457962"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /gene="dnaX"
                     /locus_tag="VII37_01430"
     CDS             complement(<1..1440)
                     /gene="dnaX"
                     /locus_tag="VII37_01430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY5068407.1"
                     /translation="MSYQVIARKWRPQKFSDLVGQEHVTETLGNAIKNDRVAHAYIFS
                     GARGVGKTTAARILAKALNCVHGPTPTPCGECDSCREIAAGTSLDVIEIDAASNRGID
                     QIRELREMVRYAPASSRSKVVILDEAHMLTGEASNALLKTLEEPPDRVIFVMATTQPE
                     DLVDTIRSRSQHFHFRALTFGEITARLEEIAQKENLKIDAGALAVISRMAEGSMRDAL
                     SLLEQTRAYCGEAITNDEVRALLGVVPDDALATLVSAIAEGSADQALGLIHTFQKEGR
                     NLQHFCREAIRHMRNLLIAKVCGANSDLIAATADQRPALAEAAALFSEEDLTRFFQIL
                     LQTDDDLRRKPDPRVHLEMGLLRLINAARLAPLEELLTEIKSGTGGSTVGSGSPIPAR
                     RAATASASGAGASATSSLKTMSAIPTATSTGAPSSPASYEAAENEPKTFAAADVIARN
                     DLAVMSARDSSTPAQILPGFRDSAGSTRAL"
     gene            complement(1506..1604)
                     /gene="ffs"
                     /locus_tag="VII37_01435"
     ncRNA           complement(1506..1604)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VII37_01435"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1721..2260)
                     /locus_tag="VII37_01440"
     CDS             complement(1721..2260)
                     /locus_tag="VII37_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5068408.1"
                     /translation="MSHHFSKVLTMVMCAVLSIALTKAQETAKKPAPTKTETPAATKA
                     ATAEAFIGTPDKIKWVQFAPGVEYGPVHGNCDKPGAPCVFQLRFAAGGKIPAHWHPVD
                     ENVTVISGTFMAGMGDSNDESKMMTLPVGTYVFMPRRMHHFAGTKDGALVQVHGVGPF
                     KINYVNPADDPSKAAKASD"
BASE COUNT          493 a          707 c          628 g          531 t
ORIGIN      
        1 gagagcacga gtcgaacccg ccgaatctcg gaaaccgggg agaatctgag ccggcgtgct
       61 tgaatcacga gcgctcatta ccgccaaatc attccttgca attacgtctg ccgctgcgaa
      121 agtcttcggc tcgttctccg cagcttcgta cgacgccggc gaagacggtg cgccagtcga
      181 agtcgcggtc gggatggcgg acatcgtttt cagggaagat gtcgcgctcg ctccagcacc
      241 tgacgcagaa gcagtcgccg cgcgccgagc gggaatgggc gatccacttc caacggtgct
      301 gccgccagtt ccacttttta tttccgtcag taattcttcc agaggcgcca gtcgcgccgc
      361 attgatcaag cgcaacaagc ccatctccaa atgcactcgt gggtccggtt tgcgtcgcaa
      421 gtcatcgtcg gtctgcaata aaatctgaaa aaaccgtgtc agatcctctt cgctgaacag
      481 cgcagcagct tccgccagag cggggcgctg gtctgcggtc gccgcaatca ggtccgaatt
      541 cgccccgcac accttggcaa tcagcagatt ccgcatatga cgaatcgctt cacggcagaa
      601 atgttgcagg ttgcggcctt ctttttgaaa tgtgtggatc agtcctaggg cctggtcagc
      661 agaaccttct gcaatcgcgc taactagcgt ggccaaggca tcgtcgggaa ccacgccgag
      721 aagcgcgcgc acttcatcat tcgtgattgc ttcaccgcag tacgcccgcg tctgctccag
      781 cagtgacagc gcatcgcgca tgctgccttc cgccatgcgc gaaatgaccg ctagcgctcc
      841 tgcatcgatc ttcaggtttt ctttctgcgc gatttcttcg aggcgcgcgg taatttcgcc
      901 aaacgtcagc gcgcgaaaat gaaaatgctg cgagcgcgac cggatggtgt ctaccagatc
      961 ttctggctgg gtggtcgcca ttacaaaaat cacgcgatcc ggtggctctt caagagtctt
     1021 cagcaacgcg ttcgaagcct cgccggtcag catgtgcgct tcgtccagga tcaccacctt
     1081 gcttcgcgat gacgccggag cataacgaac catctcgcgc aactcgcgga tttgatcgat
     1141 gcctcggtta gaagctgcgt caatctcaat tacatccagg gaagtccccg cagcaatctc
     1201 gcggcaagaa tcgcattctc cacacggagt aggagttgga ccgtgcacac agttcagcgc
     1261 tttggcgaga atgcgcgccg cggtagtttt gccaaccccg cgcgcgccgg aaaaaatgta
     1321 ggcatgcgcc acgcgatcgt tcttaatggc attcccgagc gtttcggtga cgtgctcttg
     1381 tccaactaga tcgctaaatt tttgtgggcg ccacttgcgg gctataacct ggtagctcat
     1441 ttgcgggaat cactccggtt gcgggaacag cgcgcttcac ctaacttgca aaatcttgaa
     1501 aagaggaact tcgggtgatc tgcggcacaa gaggcgttcc cggtaccgtt gctcccttcc
     1561 gggcctggcg gggttcgcgg gacctcgttg cacagagccc gaagttccac gtttcaacag
     1621 ccgcaagaat ttcttgcgtc tgctgaacga aagtctagca cagcatcgtg agcgcagcca
     1681 gattgcctgc gcccgccgcg attttcttct gtgcaaaaaa ttagtcggaa gctttggcag
     1741 ccttgctcgg atcgtcggcc ggattcacat aatttatttt gaaggggcct acgccgtgaa
     1801 cctgcaccag tgcgccatcc ttggtccccg cgaagtgatg catgcgtctc ggcataaaca
     1861 catacgttcc cacgggaaga gtcatcatct tcgactcatc attggaatcc cccatgccgg
     1921 ccataaacgt ccccgatatc accgttacat tttcgtccac aggatgccag tgcgcgggta
     1981 tttttccgcc cgcggcgaac cgcaattgaa aaacacacgg cgcgccaggc ttgtcgcaat
     2041 tgccgtgcac cggcccgtac tcgacgcccg gcgcgaactg cacccatttg attttgtctg
     2101 gtgttccgat gaacgcttcc gcggttgccg ccttggtagc cgccggcgtt tccgttttag
     2161 tcggcgcagg ttttttggcg gtttcctggg cctttgtcaa agcaatactc aacactgcgc
     2221 acattaccat cgtcaaaact tttgaaaaat gatgggacat gaatcctccg taaaaacgaa
     2281 aattggcggc acgatatcac acccccgtgt tcgggtaaac atttcggcca gccgtaactt
     2341 ttccgctcgc gtactcgca
//
LOCUS       DATCBB010000122         1397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_36301, whole genome shotgun sequence.
ACCESSION   DATCBB010000122 DATCBB010000000
VERSION     DATCBB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36301"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VII37_01445"
     CDS             <1..545
                     /locus_tag="VII37_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY5068409.1"
                     /translation="DMEASNYVDATLQIYRRALGAFPNVGVCLQAYLHRTKDDLTSLL
                     PLRPSIRLVKGAYSEPPEIAFPEKSRVNENYFLLAKEMLLAKKFYPKIRGAFGTHDTV
                     LLRRISGFAAASDLPKSDVEVQMLYGIQSGEQERLAREGYRSKVLVSYGSHWYTWFMR
                     RLAERPANLWFVARNLFHFR"
     gene            603..1190
                     /locus_tag="VII37_01450"
     CDS             603..1190
                     /locus_tag="VII37_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068410.1"
                     /translation="MTTKSKTPREVMPSVPAAAVMSFLKDTRGLLAWTTRDLAETLSI
                     PISEAAKVITIMEMQGYVKAAQGAREWFTTPAGQTVSGSKLPHYTPERVATALAGLKE
                     RIATPRQDFKSPFKISAAVAFGDFLSDRPRVQVPDVGIELVKRKSDAGSRTSATEAKA
                     SRAFLKELRGKNTPLNVQPYQEWMSKRRHQKLLRP"
BASE COUNT          355 a          377 c          382 g          283 t
ORIGIN      
        1 tcgacatgga agccagcaat tacgtggacg ccacgctgca gatttaccgc cgcgcgcttg
       61 gagcgttccc caacgtcggt gtgtgcctgc aggcttactt gcatcgcacc aaagacgatc
      121 taacaagcct gctgcccttg cgaccttcga ttcgcctggt aaaaggggct tacagcgagc
      181 ctcctgaaat cgcgttcccc gaaaaatcgc gcgtgaatga aaattatttc ttgctcgcca
      241 aagaaatgct cctcgcaaaa aagttttacc cgaaaatccg cggcgcgttt ggaacgcatg
      301 acacggtatt gttgcggcgc atttcaggat ttgcggcagc cagcgacctt ccgaagagcg
      361 acgtcgaagt gcagatgctc tatggcatcc agagcggtga gcaggagcgt ttggcgcgcg
      421 agggctatcg ttccaaagtt ttggtgtcct atggcagcca ttggtatacc tggtttatgc
      481 gccgattggc ggaacggccg gcaaatttgt ggtttgtggc gcgaaacctt tttcatttcc
      541 gctgaagatt tacgtatcgc caattatttg agttgcggtg cgcaaaagcg gttgcaatat
      601 atatgaccac caaatcaaaa acgccgcgcg aagtcatgcc gtcagttcca gcagcggcgg
      661 tgatgagttt tctgaaagac acacgaggcc tgctggcctg gacgacccgc gacctggcgg
      721 aaacgctcag tattccgata agtgaagccg ccaaagtaat caccattatg gaaatgcaag
      781 ggtacgtaaa agccgcacaa ggcgcgcgcg aatggttcac tacgcctgcg gggcaaactg
      841 tttcaggatc gaaattgccg cactataccc ccgagcgtgt cgccaccgcg ttagctggac
      901 tgaaggaacg cattgcaacc cctcgccagg attttaaatc gccatttaaa atctccgcag
      961 ccgtggcttt tggcgatttt ctcagcgatc gcccgcgcgt acaggtgccc gatgtgggca
     1021 tcgaacttgt aaagcgcaag tccgacgcag gctcaagaac atcagcgacc gaagccaaag
     1081 caagccgcgc attcctcaaa gagttgcgcg gcaaaaatac tccgctcaac gtgcagcctt
     1141 accaggagtg gatgagcaaa cgcaggcacc aaaaactgct ccggccatag ttcccttggg
     1201 agaaagaggg ccaaaattgg gggcttcacg gatggcgatg cagccttagg ccgaagcagt
     1261 attgcctgaa tgggggccag gctaatgtgc cgaacagagc cggaaatgct cgtttgcgcc
     1321 ccaaaaacgg catgctaaga tatcggcaag ttcggaacag cccgaggcca aatgactaaa
     1381 aaaccgaaga ccaccaa
//
LOCUS       DATCBB010000123         1725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_36395, whole genome shotgun sequence.
ACCESSION   DATCBB010000123 DATCBB010000000
VERSION     DATCBB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36395"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="VII37_01455"
     CDS             <1..1582
                     /locus_tag="VII37_01455"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5068411.1"
                     /translation="VRVVQTETGGAFGGKEDYPSTLASHAALLAMKSGHPVKMVYDRM
                     EDLAATTKRHPSRTRHRTALDKDGKLLAMDIELATDGGAYATLSSTVLSRATLHAPGP
                     YSCPNVRVRAKSWATNTVPYGAFRGFGAPQAIFALERHMDEVAAALGIDPVEFRRRNF
                     LHDGDTTATEQVMREPVIMDMLLERALRESDYHARRARFARENPSSAVKRGLGIACFY
                     HGSGFTGSGERILNSLAGMDVTPEGKVRVLVSNTEFGQGTNTVLTQIAAESIGIDYED
                     IIMAAADTGMVPNSGPTVASRTVMVVGRLIERAGLQLVEMLREHAGLGAKFTREEFFA
                     ACERYRAAKGEVASLVRYEAPPGIFWDDEKYRGEAYPSYAWSIHVAQVAVDTVTFSAE
                     VEEFWAVQEVGRVLNPVLATGQIEGGIAQGIGYALYEKVILQNGHMANNQMTNYIIPT
                     AEDVPPIHVFFEEIPFRYGGYGAKGIGELPHDGPAPAIVNAIKDATGISFHAIPLLPE
                     DLFAGLAAHQQVEEQATL"
BASE COUNT          365 a          475 c          542 g          343 t
ORIGIN      
        1 agtgcgggtg gtgcagacgg agaccggcgg cgcgtttggc ggaaaggagg actatccttc
       61 gacgctcgcg tctcatgcgg ctttgctggc catgaagagc gggcatccgg tcaagatggt
      121 ttacgaccgc atggaggatc tggccgcaac tacaaagcgc catccgtcgc gcacgcggca
      181 tcgcaccgcg ctcgacaaag acggaaaact gctggcgatg gatatagaac ttgcgacgga
      241 cggcggggct tacgcgacgc tctcctccac tgtgctgtcg cgcgcaactc tgcatgcacc
      301 gggtccttat tcgtgcccca acgtgcgggt acgcgcgaaa tcatgggcaa ccaatacagt
      361 tccctacggg gcgtttcgcg gattcggagc gccacaggcc atcttcgcgc tcgagcggca
      421 catggatgag gtcgccgctg cactgggcat cgatccggtg gaatttcggc gcagaaattt
      481 tctacacgac ggagacacaa cggcaacaga gcaggttatg cgcgagccgg tgatcatgga
      541 tatgttgctc gaacgggctc tgcgggagag cgactatcac gccaggcgtg cacggtttgc
      601 gcgtgagaat ccatccagtg cggtcaagcg cggcctcggc attgcctgct tctatcacgg
      661 ctccggtttt accggctccg gtgaacgcat tttgaattcg cttgcgggaa tggatgttac
      721 gcctgaggga aaagtgcgcg tgctggtgtc gaatactgaa ttcgggcagg gcacgaatac
      781 tgtgctcacg cagatcgcgg ccgagtccat tggcattgac tacgaagaca tcattatggc
      841 ggctgcggat accggcatgg tcccaaacag cggccccact gttgcgtcgc gcacggtgat
      901 ggtggtgggc cggctgattg aacgcgccgg gctgcaattg gttgaaatgc tccgcgagca
      961 tgccggcctc ggcgcaaagt tcacgcgcga agaattcttt gcggcttgcg agcgctatcg
     1021 tgcagcaaag ggtgaagtcg cttcactcgt ccgctacgaa gcaccgccag gcattttctg
     1081 ggacgatgag aaatatcgcg gcgaggcgta tccctcttat gcgtggtcca ttcacgtggc
     1141 gcaagtggcc gtggatacgg ttacgttttc tgctgaagtg gaggagttct gggcggtgca
     1201 ggaggtgggg cgagttctca atccggtgtt ggcgacaggg caaatcgaag gaggcattgc
     1261 gcagggcatt ggctatgcgc tctacgaaaa agtcatcctg cagaacgggc acatggccaa
     1321 caaccaaatg acgaattaca ttattccaac cgctgaagat gtcccaccga tccacgtgtt
     1381 tttcgaagag attccgtttc ggtatggcgg ttacggcgca aaaggcatcg gtgaactacc
     1441 tcacgacggg cccgcgcccg caatagtgaa cgcgatcaag gacgccacgg gcatcagctt
     1501 ccacgcgatc cctctgctgc ctgaggatct gttcgcggga ctcgccgctc accagcaagt
     1561 cgaggagcag gcaaccctat gagtacgtcc tggcctacaa ttcatttcac catcaacggc
     1621 aagagttgcg aagttcaagc gccgccgatg aagcgcctgc tcgatgtgct gcgagaagac
     1681 cttcatctca ccggaaccaa ggaaggttgc ggcgaaggag agtgc
//
LOCUS       DATCBB010000124         1952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_68152, whole genome shotgun sequence.
ACCESSION   DATCBB010000124 DATCBB010000000
VERSION     DATCBB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68152"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1952
                     /locus_tag="VII37_01460"
     CDS             102..>1952
                     /locus_tag="VII37_01460"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068412.1"
                     /translation="MAVSDNITKRPKVVESESIVVRGARVHNLKNIDFDIPHNALTVV
                     TGVSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLERIEKPDVDLIDGIAPAIAIRQ
                     KNTTRNPRSTVATATEIYDYLRLLYARVGRTFCRNCGQEVKKDTVDEVAQTILSLDEG
                     TRLNVLFPVEALTPPAAKPEKKTRGRKSAKAPAVSETVVNALRERLFDLRKKGFGRLF
                     QNGRIFEFATPESLLDIDFGQPVYVLIDRIVVGPDQRTRIVDALETGYREAGETVFEL
                     APPPQPEIDAASDADFDDLVMNVTLMQWRFSHRFECKNCHIKYEEPEPPLFSFNNPFG
                     ACPRCQGFGNTIDFDLNLVIPDVTKTLSEGAIEPFTKPKYRPYQAELRKFARQSGVPF
                     DVAWQDLTAEQQQLVMEGESKGKYPGIQGFFAMLERKKYKLHVRVFLSRYRGYAVCPE
                     CGGGRLRADALQVKIDGRNIRELTQLTVEAAAEFFKTLTLTPEKAGIAGKILEQIQER
                     LKFLNDVGLEYLTLDRMASTLSGGEAQRIQLATSLGSRLVGALYVLDEPSIGLHSRDT
                     ARLVTILHELRNLGNTILVVEHDPEVMRAADLILDLGPGAGENGGRVMASA"
BASE COUNT          439 a          449 c          653 g          411 t
ORIGIN      
        1 caccttcgca caaaggtccg cgcatgatcg aaagcggtga tttgtgctta gttcgggacg
       61 gcacatcgtg agggatggga gtcgaattag ggttcgaaaa aatggctgta tctgacaaca
      121 tcacgaaaag accaaaggta gtggaaagcg agagcattgt tgtgcgcgga gcgcgggtgc
      181 acaatctcaa gaacattgat tttgatattc cgcacaatgc gctgacggtg gtgacgggcg
      241 tttccgggag cggcaagagt tccctggcct ttgacacgat ttatgccgag ggccagcgac
      301 gctatgtgga gtcgctatcg gcgtatgcgc ggcagtttct ggagcgcatt gagaagcccg
      361 acgttgacct gattgatggc attgcaccgg cgattgcgat ccggcagaag aatacgacgc
      421 gcaatccgcg gtcgacggtg gcgacggcca cggagattta cgactacctg cgcttgctct
      481 atgcgcgcgt gggccgcacg ttttgccgga actgcggaca ggaagtaaag aaagataccg
      541 tggacgaagt ggcgcagacg attctgtcat tagacgaagg cacgcggttg aatgtgctct
      601 tccctgtgga ggcattgacg ccgccagcgg cgaagccgga aaagaaaacg cgtggacgga
      661 aatccgcaaa agctcccgca gtttccgaga cggtggtgaa tgcgctgcgc gagcgcttgt
      721 tcgatttgcg aaagaaaggt tttggccggc tcttccagaa tggacggatc tttgagtttg
      781 caacgccgga gtcgctgctg gatatcgatt tcggccagcc ggtgtatgtg ctgattgatc
      841 gtatcgtcgt ggggccggat cagcggacaa gaattgtgga cgcgctggag acgggatatc
      901 gagaagcggg agagactgtg ttcgagctgg cgccaccgcc acagcccgaa atagatgccg
      961 cgtccgacgc tgattttgat gatttggtaa tgaacgtgac attgatgcaa tggcgcttca
     1021 gtcatcgttt tgaatgcaag aactgccaca ttaaatatga agagccggag ccgccgctgt
     1081 tttcatttaa caatcctttt ggcgcgtgcc ccaggtgcca gggatttggc aacaccattg
     1141 attttgattt gaacctggtg attccggacg tgacgaagac gctgagtgaa ggcgcgattg
     1201 agccttttac caagccgaag tatcgtccgt accaggcgga gttgcggaag tttgcgcggc
     1261 agagcggcgt gccgtttgat gtcgcgtggc aggacctgac ggcggagcag cagcagctgg
     1321 tgatggaagg cgaaagcaag ggtaaatatc ctggcataca gggatttttt gccatgctgg
     1381 aacgcaagaa atacaagctg catgtgcgcg tgtttttgag caggtatcgc gggtacgcgg
     1441 tgtgtccgga gtgcggcgga ggcaggctgc gcgcggatgc gctgcaggtg aagattgatg
     1501 gcaggaatat ccgcgagctg acgcagttga cggtggaagc tgccgcggaa tttttcaaga
     1561 cgctgacgct tacaccggag aaggcgggga ttgcaggaaa aattctagag cagattcagg
     1621 aacgcctgaa attcctgaac gatgttggat tggaatatct gacgcttgac cgcatggctt
     1681 caacgctgag tggcggcgag gcgcagagaa ttcagctggc gacgtcgctc ggctcgcggc
     1741 tggttggcgc gctgtacgtg ctggatgagc cgtcgattgg gctgcacagc cgtgacaccg
     1801 cgaggctggt gacgatcctg cacgaactga gaaatctggg caatacaatt ttggtagtag
     1861 aacacgatcc tgaagtcatg cgcgcggcgg acttgatcct ggacctgggg ccgggcgcgg
     1921 gcgaaaatgg cgggcgcgtg atggcgtcgg ca
//
LOCUS       DATCBB010000125         1126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_230703, whole genome shotgun sequence.
ACCESSION   DATCBB010000125 DATCBB010000000
VERSION     DATCBB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230703"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..503)
                     /locus_tag="VII37_01465"
     CDS             complement(213..503)
                     /locus_tag="VII37_01465"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY5068413.1"
                     /translation="MPAKKLSDAEVTERMGQIPGWSLAGGKLHRAFECNDFVSAFGNM
                     TRVALVAESMNHHPEWFNVWNKVVIDLNTHSVGGISDLDFKLAGKISEIFGG"
     gene            759..>1126
                     /locus_tag="VII37_01470"
     CDS             759..>1126
                     /locus_tag="VII37_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068414.1"
                     /translation="MVRSRSKSKKKKNLPPPPPAQRGGVELVDPRVQAQLKVYDEALS
                     LFRQQKFARAKQELEKVVEGPSKELADRARMHVKIAEQRMKPSHDQNPRTADEHYQRG
                     VAMMNVGRWDDARESLDKAR"
BASE COUNT          266 a          372 c          265 g          223 t
ORIGIN      
        1 agcgctggga ctataagttt tttgcaggta gtgcgcgtcg gaagccgcca gaacagcatt
       61 atcgcactgc ggcgaatagc cgctggaact cattgtggcg cactgccggt aggagtcaag
      121 cgtcaagggg gccccatttc cgccacggta ccctgcgatt tgtgggtcgt gcgtttaggc
      181 gcgacatcag caaatctgga gcaaccatat ccttacccgc caaatatctc gctaattttc
      241 cccgccaatt taaaatctag gtcactgatc ccgccaacgc tatgcgtatt caaatcaatc
      301 actactttat tccacacgtt aaaccattca ggatggtgat tcatcgactc ggcaaccagc
      361 gcaacacgag tcatgttccc gaaagcgctc acaaaatcgt tgcactcaaa cgcgcgatgc
      421 agcttcccac cggccaaact ccaccccggt atctgcccca tccgctccgt aacttcagca
      481 tcgctaagct tcttcgccgg catcgccatc ctccattccc ttgcgccatc gattatgcgg
      541 cggccaccgc gcagcagcaa ccctcagata cgcgggttcc gtgaatcagt actcatcgtc
      601 aacagtttca gcggtttaca aactaccttg ccgccgccgt gcacaagttc ctgtgccact
      661 aattgacccc tacattgacg cgccatagta cctctgttag catgccctcc atggcaaaag
      721 ctcgtaagcc aactcgcgct tcctcccact ccaagagcgt ggtccgttct cgctccaagt
      781 caaagaagaa aaagaactta ccgccccctc ctccggcgca gcgcggcgga gtcgagctcg
      841 tcgacccgcg cgtccaggcc cagctcaaag tctacgacga ggcgctgtca ctatttcggc
      901 agcagaaatt tgcccgtgcc aaacaggaac tcgaaaaagt cgtcgaaggc cccagcaaag
      961 aactcgccga ccgcgcccgc atgcacgtga aaattgccga gcagcgcatg aagccctcgc
     1021 acgatcagaa tccccgcact gccgatgaac attatcagcg cggcgtggcc atgatgaacg
     1081 tgggccgctg ggatgacgcc cgcgaaagtc tcgacaaagc gcgcca
//
LOCUS       DATCBB010000126         1426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_3814, whole genome shotgun sequence.
ACCESSION   DATCBB010000126 DATCBB010000000
VERSION     DATCBB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3814"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VII37_01475"
     CDS             complement(<1..287)
                     /locus_tag="VII37_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068415.1"
                     /translation="MPDHISRKELKHDKIKETLEHGAKAVYSHSELTLAVLLVVLAAA
                     AVYGGWRIYHDRKTAEANAAFDAAQKVYGARVGPPAPGEPVDPSAPTFPDEA"
     gene            347..1126
                     /locus_tag="VII37_01480"
     CDS             347..1126
                     /locus_tag="VII37_01480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5068416.1"
                     /translation="MNRISACLITYNEEHNLPRALASLAGVVDEIVIVDSGSIDHTEE
                     VAREHGATIHFREWSSYGEQKNFAAECAQNEWIFSMDADEELSSALQTSLLAWKKSEP
                     KFPVYEVARKTWYLGAWINHSGWYPDFQRRLYNRDEARFSGIIHESVRFVGPPGRLHG
                     DLLHYTVRTFKEHEAKVEKYADLSARQMHWEGKRQWRFAMLLVAPWRWFYTFILRGGF
                     LDGYRGALIARMAARSVRLKYAKLGKLIQTKKLERERRPSS"
     gene            1123..>1426
                     /locus_tag="VII37_01485"
     CDS             1123..>1426
                     /locus_tag="VII37_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068417.1"
                     /translation="MNILLVDLETEWRGGQNQALLLLKALNARGDCAELVTVRGSELG
                     KRAARRRMKVHFVMARAARVSAALKIRSLTNRGSFDVVHANEAHAVTAAWLAGAHRR"
BASE COUNT          309 a          362 c          438 g          317 t
ORIGIN      
        1 gcttcgtcgg ggaacgtagg cgcgctgggg tcgacgggtt cgccaggggc gggcggaccg
       61 acacgcgcgc cataaacttt ctgggccgcg tcgaaggccg cgttcgcttc cgcggtttta
      121 cggtcgtggt aaattcgcca accaccataa acggcggcag ccgccaggac aaccagcaag
      181 actgctagtg tgagctcact gtgggaatat acggctttcg caccatgttc cagggtctct
      241 ttgattttgt catgcttcaa ttctttgcgt gaaatatgat ccggcacgaa cgttttcctc
      301 gcttgtctgg aattggtaat cgtagcacag taggggctgc ccgctcatga accgcatctc
      361 tgcttgcctg attacttata acgaggagca taatctgcct cgcgcgctgg cgtcgctggc
      421 gggggtggtg gacgaaattg tgatcgtgga ttccggtagc atcgaccaca cggaagaagt
      481 tgctcgagaa catggcgcca cgattcactt ccgggaatgg tcgagttatg gggagcaaaa
      541 gaattttgcc gcggagtgcg cgcaaaatga gtggatcttt tctatggacg ccgacgaaga
      601 gttaagcagc gcgctgcaaa catctttgct ggcctggaaa aaatccgaac ccaaatttcc
      661 ggtttatgaa gtggccagaa aaacatggta cctcggcgcg tggatcaacc attccggctg
      721 gtatccggat tttcaacggc gcctttacaa tcgcgacgaa gcgcgattct cgggaatcat
      781 tcacgaatcg gtgcgctttg tcggaccgcc cgggcggctc catggtgatt tgctgcatta
      841 cacggtgcgc actttcaagg agcacgaagc gaaggtagag aagtacgcgg acctctcagc
      901 ccggcaaatg cattgggaag gcaagcggca gtggcgattt gcaatgctat tggtggctcc
      961 ctggcgctgg ttttacactt ttatcctgcg cggtggtttt ctcgatggtt atcgcggagc
     1021 gctaatcgcc agaatggcgg cgcgctcggt acgcctgaaa tacgcgaaac tgggaaaatt
     1081 aatccaaacc aaaaaactcg aacgtgagcg caggcccagt tcgtgaatat tctgcttgtg
     1141 gatctcgaaa cggaatggcg cggggggcaa aaccaggcac tgctattgct gaaagccttg
     1201 aacgcgcgag gtgactgcgc agagctggta accgtgcgcg gctccgagct gggcaagcga
     1261 gctgctcgca ggcgcatgaa agtgcatttc gttatggcgc gcgcggcgcg ggtttcggca
     1321 gcgttgaaaa ttcgttcgtt gacaaaccgt ggttcattcg atgtggtgca tgccaatgaa
     1381 gcacatgctg taacggccgc ctggctggcg ggagcgcacc gtcgat
//
LOCUS       DATCBB010000127         1798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_36524, whole genome shotgun sequence.
ACCESSION   DATCBB010000127 DATCBB010000000
VERSION     DATCBB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36524"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1270..1608
                     /locus_tag="VII37_01490"
     CDS             1270..1608
                     /locus_tag="VII37_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HEY5068418.1"
                     /translation="MKRDLYAEVSTRIVAELERGAAPWIKPWSATAGMNTPCNAATNR
                     PYSESNVVLLWMAQTAGYRTPRYLTFKQALDLGGNVRNSEPGKLAISAPWQLLWRPPS
                     PLSSRYPRTL"
BASE COUNT          398 a          506 c          515 g          379 t
ORIGIN      
        1 gagggtgaaa ttactgacgc cgctagtcgt aggggtgccg ctgatattgc ctgtcgaagt
       61 gtcgagtgtg aggccgttgg gcagcgcccc ggaagtgacg gcgaacgtgt agggcgcgct
      121 accgccattg gccataacgg cttgatcgta acccacgccc acaccaccgc cgggcaagct
      181 cgctggagaa agcgttatcg cggccgacgc gccattcgca cccaacacaa tgaatatggc
      241 taatatgaac ccacaacggc gaagtagaag atcgcgccga aaagcgttgc caattttcca
      301 tgtaaacatt tttggcagcc aaggccggga cttcggccaa aatcgcgcgc caaatttgga
      361 ttattgtggt taacggttgg ttaaatggtt aataggcggc aaattgcctg cgctaccgtt
      421 tccagtcgcg atcgcagttt gccctctagg ttttcggatg cgccagctcg aacgaggaac
      481 cgaccaccat atttgcgaaa tgcgaccgcg ttcgcggcct gataggactt gtagcctgcc
      541 gcatcactca catcgacgtg tgcgatccaa tatcctttag ccaacctcgc gcgcgctcgt
      601 gagatgctcg tcgtcgcaaa atcccaaagc gtgccgcccc gtgcattgag ttcaaagact
      661 acgacaccgc gctcgcctgt tatcgctcgc ccgaatacgc tgctgcgata gaaaagcgga
      721 aagggaaggg cgaaatagat ctagccattg tcgatggcta tgacagcgtg caacctggat
      781 agaactgtct taatacgaac tgccgttatg gccacttttg gcacatgacc gcgttcaccg
      841 cgacgcacca tttcggacgc gattggacaa caacggacaa acgtcaattt tggcccgcga
      901 cggctatgac gtgaacgatg cgctggacgg cgcccgctct gcggcatcag agtgccagag
      961 ggtggttgcg ctgaagcaag ccacatttag gggagccgtc cagacgctcg cggagatgca
     1021 gagagcaacc gcgaagttta aattttatta acgatttggt ttttggttcc tacaaaattt
     1081 tgaccctagt gtttgggcca cccgtaccac ccccgcctgg ccagcatacc ggggggtggc
     1141 gccctgctcc cctgagaccg tcagcctcgc atcacgctgc aaaacttaga aattttgtcc
     1201 gacgcggcaa tggtgtgcgg ctgttcgcaa aagacggtcg cgcgtggctg taactgcgag
     1261 ggtggtgcta tgaaacgtga tctctatgca gaggtatcga cgcgcattgt cgcggagttg
     1321 gagcgtgggg cggcgccatg gatcaagcct tggtcggcaa cggccggcat gaatacgccg
     1381 tgcaacgccg caaccaatcg gccttactca gaaagcaacg tcgtgctgtt gtggatggcg
     1441 caaacggccg gctatcgcac gccgcgctat ctcactttca agcaggcctt ggatttgggc
     1501 ggcaacgtgc gaaatagcga acctggaaag cttgctatat cagcaccttg gcagttatta
     1561 tggcgtcctc cctctccgct cagcagccgt taccctcgca cgctgtaacg gagcgtagcg
     1621 agttcagcac gtggtggtcc ggtgggggtg aagcccagaa acgccgccga ttgcttctgc
     1681 ggcgaggctt aaaccgatct tacgcccttt gccgttttag tcggtttttg accgctgcag
     1741 gcggagaacg atctcgacga tgaattgcta gccgagccat cataaactcc cacagccc
//
LOCUS       DATCBB010000128         2406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_166259, whole genome shotgun sequence.
ACCESSION   DATCBB010000128 DATCBB010000000
VERSION     DATCBB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166259"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VII37_01495"
     CDS             <1..323
                     /locus_tag="VII37_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5068419.1"
                     /translation="LAEYARRRRRIVDGLRAIPGITCEDPGGAFYAFPNISAHLQGNN
                     PLAKSCTEITKLLLDKVQVAVVPGEAFGAPGYLRLSYATSIERIDEGLRRLGRFFSRA
                     EAAT"
     gene            362..1381
                     /locus_tag="VII37_01500"
     CDS             362..1381
                     /locus_tag="VII37_01500"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEY5068420.1"
                     /translation="MLRIWGARSLAPIYPDKINLAESIGEAWLTGPECKIETGPLAGP
                     LHQAWQKMPSEWRGTDFASQQEPREFPILVKFLFPTDQLSIQVHPDDAYAKNNEPPGA
                     VGKTEMWHAVSANPGATLLLGLVPEVDREKFQSAIKQGGLESFFERLPVSPGDTFFVP
                     SGTPHTIGQGMILCEVQQNSDLTYRLYDFHRVDAKGNPRELHIEKAMAVIDFDHRKGG
                     KVAPVALSTTDGAKQYLLAACPYFASERWDLASQHQANSEPTRFELLVILSGRGNIKW
                     RGDSCSYQQGECWFIPANLGEFSVVPERPTSILRTYVPVISKLLNNLSRGSVPNSAIE
                     QVLFT"
     gene            1389..>2406
                     /locus_tag="VII37_01505"
     CDS             1389..>2406
                     /locus_tag="VII37_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEY5068421.1"
                     /translation="MAAEKLTAHAVILAGGRGTRFWPRSRTRTPKQLLNIIGADSMLQ
                     QTVARLAPFIPADRIWTVTNAEQSASVRKHLPASARKHILAEPVGRSTAAAIALAALH
                     IRHTESGDALMAVLPADLYIAQPKKYAKIAHLALKVAREPGRMVVLGIPPTRPETGFG
                     YIQCASKPASSSPFPVFPVRAFKEKPDLPVAKKYVASGHYRWNAGMFFWRVSTFLDAL
                     KKYLPKTYAALDSLAATIGTRKYENKLRSIYPRLENIAVDYAILEPATCEKGPSRVFM
                     IPADIGWSDIGSWTAVHEQSSSATHQNVLAGAGHMLEAEGNFIWSPTKFVAAIGIRDL
                     VVVET"
BASE COUNT          537 a          806 c          553 g          510 t
ORIGIN      
        1 tgctcgccga atatgcgcgc cgccgcaggc gcatagtcga cggcctgcgc gctattcccg
       61 gtattacctg cgaagatccc ggcggcgcgt tctacgcctt tccaaatatc tccgcgcacc
      121 tccaaggaaa taacccgctg gccaaatcct gcaccgagat caccaagcta ctgctcgata
      181 aagttcaggt cgccgtcgtt cctggagaag ccttcggcgc acccggctac ctccgccttt
      241 cttacgccac ttccatcgag cgcatcgacg aagggttgcg ccgcctgggc cgttttttct
      301 cccgcgctga agccgccact taatcgtgcg gccccagccc acacgcattg agcccaaatt
      361 catgctccgt atctggggcg cgcgttcgct cgctcccatt tacccggata agataaatct
      421 agccgaatcc ataggcgaag cctggctcac cggaccagaa tgtaaaatcg aaacaggccc
      481 gttagcaggc cctcttcatc aggcgtggca aaaaatgccg tccgaatggc gtggcaccga
      541 tttcgcatct caacaagagc cgcgcgagtt ccccattttg gttaaatttc ttttccccac
      601 cgatcaactt tccatccagg ttcaccctga cgacgcctat gccaaaaata acgagccccc
      661 gggcgcggta ggaaaaacgg aaatgtggca cgctgtctcc gccaatccgg gcgccacgct
      721 tttgctcggc ttggtccccg aagtagatcg agaaaaattt cagtcagcga taaaacaggg
      781 cgggctggaa tctttttttg agcgcctgcc ggtctcaccc ggcgatacct ttttcgttcc
      841 ctccggaacc ccgcacacca tcggccaggg catgatcctc tgcgaagtgc agcagaactc
      901 cgacctcact tatcgcctct acgacttcca tcgcgtcgac gcaaaaggca atccccgcga
      961 actgcacatc gaaaaggcaa tggccgtcat tgattttgat catcgcaaag gcggaaaagt
     1021 cgcgccagtt gcattatcta caaccgatgg cgcgaaacaa tatttactcg cagcctgccc
     1081 atatttcgcc agcgaacgat gggaccttgc gtcgcagcac caagcaaatt ccgagccaac
     1141 ccgcttcgaa ttattggtaa ttcttagcgg tcgcggcaat attaaatggc gcggcgattc
     1201 ttgttcctac cagcaaggcg agtgctggtt tattcccgca aatcttggcg aattttccgt
     1261 cgttcccgag aggcccacgt ccattttgcg cacttacgtg ccggtcatca gcaaattact
     1321 taacaattta tctcgcggaa gcgtgccaaa ctccgccatc gaacaagtgt tattcacctg
     1381 atccaatcat ggccgcagaa aaactcaccg cacacgctgt aatcctcgcc ggtggccgcg
     1441 gcacccgctt ctggccgcgc agccgcacac gcacacccaa gcaactactc aacatcatcg
     1501 gcgcggactc catgctgcag caaaccgtcg ctcgcctcgc accattcatt cccgctgacc
     1561 gcatctggac cgtcaccaac gccgaacaat ccgcatccgt tcgcaagcac ttgcccgcat
     1621 ccgcgcgcaa acacatcctc gccgaacctg tcggccgcag cactgccgca gccatcgccc
     1681 ttgctgcact gcacattcgt cacaccgaaa gcggcgacgc cttaatggcg gtgctccctg
     1741 ccgatctcta tatcgcccaa cccaaaaaat acgccaagat cgctcatctc gcgctcaagg
     1801 ttgcacgcga acccggccgc atggtcgtcc tcggcatccc acccactcgg ccagagaccg
     1861 ggtttggcta tattcagtgc gcttctaaac ccgcttcatc ctcgcctttt ccagtcttcc
     1921 ctgttcgagc cttcaaggag aagccggatt tgcctgtggc aaaaaagtac gtcgcctcag
     1981 gccattaccg ctggaacgcc ggaatgtttt tctggcgtgt ttccaccttt ctcgatgcgc
     2041 taaaaaaata tttgcccaaa acttatgcag cgctggactc tctcgccgcc accatcggca
     2101 cgcgtaaata cgaaaacaaa cttcgctcca tctatccgcg cctggaaaac attgccgtcg
     2161 actacgccat tctcgaaccc gccacttgcg aaaaaggccc gtcccgtgtc ttcatgattc
     2221 ccgccgacat cggctggagc gatatcggtt cctggaccgc cgttcacgaa cagtcctcca
     2281 gtgccactca tcaaaatgtc ctggccggcg ccggccacat gctcgaagca gaaggcaatt
     2341 ttatttggag tcccacaaaa ttcgtcgccg ctatcggtat acgcgatcta gtagtggtcg
     2401 aaacgc
//
LOCUS       DATCBB010000129         1320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_133771, whole genome shotgun sequence.
ACCESSION   DATCBB010000129 DATCBB010000000
VERSION     DATCBB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133771"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1098)
                     /locus_tag="VII37_01510"
     CDS             complement(76..1098)
                     /locus_tag="VII37_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEY5068422.1"
                     /translation="MVKLNYFRAHSRRTLVAATALVLLAVGAVAAFAPPFGKWTRLST
                     EPVLSPQGNGFESAGTFNPSVVKKDGQFVMLYRAQDHQGTSSLGYATSEDGIHFNRRP
                     TPVLTAEASYEKGGGVEDPRLQKFGDTYYLTYTGYNNVDGAAADKKDAQLCLATSKDL
                     IRWERHGIIIPAYKGKWNVKWTKSGAMVPEKINGKYWMYYLADAKGKDSQMGVAFSED
                     LLHWTEALDHPVLASRPGSFDSQVVEPGPPPVITSEGIFLIYNGAADNLVYSTGWALF
                     DKKDPTKVLARSDQPMFGPELEWEKVGQVPNVVFVEGAVHDGKRWLFYYGGADKTVGV
                     ATAPVR"
BASE COUNT          297 a          365 c          348 g          310 t
ORIGIN      
        1 aaaaataaca gaattcacga cgctaaaagc tgtcgcctga cgtgatttga caatgcgtcg
       61 attaagaagg gcgggtcagc gcaccggagc ggttgctacg ccaacggttt tgtccgcgcc
      121 gccatagtaa aagagccagc gcttgccgtc gtgcaccgca ccttccacga acacaacatt
      181 cggaacctgc ccgacttttt cccattctag ctccgggcca aacatgggct gatcagagcg
      241 tgccagaact ttggtcggat cttttttgtc aaacaacgcc cagccggtgg aataaacaag
      301 attgtcggcg gcgccgttgt agatcaaaaa tatgccttcg ctggtgatta ccggtggcgg
      361 ccctggctcc acgacttgtg aatcgaatga tccggggcgg ctggccagca cgggatggtc
      421 caatgcttcc gtccagtgca acaaatcctc agaaaacgcc acgcccattt ggctgtcctt
      481 gcctttggcg tccgccagat aatacatcca atacttgcca ttgatttttt ccggaaccat
      541 cgcgccagac ttagtccact tcacattcca tttgcccttg tacgcaggaa tgatgatccc
      601 gtggcgctcc caacgaatca gatccttgga agttgccagg cagagctgtg cgtctttttt
      661 gtcggcggct gcgccgtcga cgttgttgta tcccgtgtac gtcaaataat aggtgtcgcc
      721 aaatttttgc aatctgggat cttccacgcc gccgcccttt tcataggaag cttccgccgt
      781 cagcactgga gttggccggc gattgaaatg aatgccgtct tcgctggtgg cgtatcccag
      841 cgaagaggtg ccctgatgat cctgagcgcg atagagcatc acgaactggc catctttttt
      901 gaccactgag ggattaaatg ttccggccga ttcgaatccg ttcccttgcg gggaaaggac
      961 gggctcggta gaaaggcgtg tccattttcc aaatggcggt gcgaaggcgg ccacggcgcc
     1021 gaccgccagc aataccagtg ctgtcgcggc gaccaaagtt cgtcgcgagt gtgctcgaaa
     1081 ataattaagt ttcaccattt gccaaaattc ctttccttac cattgatcgg aaattccaag
     1141 ctttgtctcg acgcaaaata aaaataactt gtggatcaga attcggcctg caattgcagg
     1201 aaggctttgc tcgcaatctg caaacgcgca tcaacaggct tgccattggc agtcaagcgc
     1261 gcggttcgct ctcgcgccgc cggaatcgtg atcaccgcag atcgggtggc aggcgtggga
//
LOCUS       DATCBB010000130         1455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_198592, whole genome shotgun sequence.
ACCESSION   DATCBB010000130 DATCBB010000000
VERSION     DATCBB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198592"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..>1455
                     /locus_tag="VII37_01515"
     CDS             77..>1455
                     /locus_tag="VII37_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY5068423.1"
                     /translation="MSVFSLVAALFFARHFIGAEQGTPDMKKIAGAIKEGAEIFLKRQ
                     YKAVAALLCLLLPTFAYAQPEQAGGGEANLVLPDLSTVSFFGMNGHALLMIGLLFCAG
                     GLLFGLAIYVQLKNMPVHRSMLEISELIYETCKTYLVTQGKFILLLWAFIAVIISLYF
                     GWLAPVPNKPILVTLPIILLFSLVGIAGSYGVAWFGIRVNTFANSRTAFAGLRGKPYA
                     ISEIPLKAGMSIGMLLISVELLIMLFILLFIPRDYAGPCFIGFAIGESLGAAALRIAG
                     GIFTKIADIGADLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGV
                     ALITFILLAVKSPETQAQLLVWIFVMRVAMLISSSGAYFLNGAIARAKYANADEMDFE
                     APLTSLVWMTSIVSIILTYIVTYLMIPNLGDGTLWWKLASIISCGTLAGALIPELVKV
                     FTSTKSTHV"
BASE COUNT          280 a          392 c          416 g          367 t
ORIGIN      
        1 gtgggtgata cggcttcaaa ccataacttg ggaggattta gtgatagcac agggttgggt
       61 gtactttttt ctggccgtga gcgttttttc gctcgttgcg gccttgtttt tcgcgcgcca
      121 ttttattggc gcagagcagg gaacaccgga tatgaagaaa atagccgggg ccatcaaaga
      181 aggcgctgag atattcctca agcgccaata caaggccgtg gcggctcttt tgtgcctgct
      241 gcttccgaca ttcgcctacg cgcagccgga acaggccggc ggaggcgaag ccaacctggt
      301 actgccggat ctgtcgaccg tgagtttttt cggcatgaac ggacatgccc ttctgatgat
      361 cgggttgcta ttctgcgccg gcggcctgtt gttcggcctg gcgatctatg tgcagttgaa
      421 gaacatgccg gttcatcgct cgatgctcga aatttccgaa ctgatttatg agacatgcaa
      481 aacgtacctg gtgacgcagg gcaagttcat tcttctgctc tgggcattca tcgcggtcat
      541 tatctcgctg tactttggct ggctggcgcc ggtgccgaac aaaccgattt tggttacgct
      601 gccgatcatt ttgttatttt cgctggtcgg catcgcgggc agctacgggg tggcgtggtt
      661 cggaattcgc gtcaatacat ttgcgaactc acgaacggca ttcgcaggat tgcgcggcaa
      721 gccgtatgca atctctgaga ttccgctgaa ggccggcatg agcatcggca tgttgctgat
      781 ctcggtcgag ctgttgatca tgctcttcat ccttttgttc atcccgcgcg attatgcggg
      841 cccatgcttc attggcttcg cgattggcga gtcactgggt gccgcggctc tgcgtattgc
      901 cggcggcatt ttcaccaaga tcgccgacat tggcgccgat ctgatgaaga tcgtattcaa
      961 gatcaaggaa gacgatgcgc gcaacccggg tgtaatcgcc gactgcacgg gtgataacgc
     1021 gggcgactca gtaggcccca gcgccgacgg gtttgaaacc tacggcgtca ccggcgtggc
     1081 cttgatcacc ttcattttgc tggcggtcaa gagtcccgag acgcaagcgc aactgctggt
     1141 atggatcttc gtcatgcgcg tggccatgct gatctccagt tctggcgcct acttcctcaa
     1201 tggcgcaatc gctcgcgcga aatacgccaa cgccgacgaa atggattttg aggcgccgtt
     1261 aacctcgctg gtttggatga cgtccatcgt ttccatcatc ctgacctaca tcgtcacgta
     1321 cctgatgatc cccaatctgg gggacggaac gttgtggtgg aagctggctt caatcatctc
     1381 gtgcggaacc ctggcgggcg cgctcatccc cgaattggtt aaggttttca cttcaaccaa
     1441 atccacccac gtcaa
//
LOCUS       DATCBB010000131         1735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_263853, whole genome shotgun sequence.
ACCESSION   DATCBB010000131 DATCBB010000000
VERSION     DATCBB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263853"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VII37_01520"
     CDS             complement(<1..941)
                     /locus_tag="VII37_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5068424.1"
                     /translation="MQTRKLGNSDLNITRVGFGAWAIGGGGWEFGWGNQEDNDSIAAI
                     KRALELGVNWIDTAAVYGTGHSEEVVAKALKEWKGARPYIFTKCSMVWDEQRKVRNEY
                     SADSIRRECEASLRRLQVSVIDLYQMHWPPADNGAGLEEAWQTMATLQKEGKVRWIGV
                     SNFNVEQLKRAEKIAPVTSLQPPYSLIRRQIEAEILPYCEKQGIGVISYSPMASGLLT
                     GAMTRERAAALPANDFRSRGAEFKEPRLSKNIELVERLRQVGQRLGRSPGEIAIAWVL
                     RHPAITGVIVGARNAKQVEGVVGAADVTLSKQDIAEI"
     gene            complement(1013..>1735)
                     /locus_tag="VII37_01525"
     CDS             complement(1013..>1735)
                     /locus_tag="VII37_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY5068425.1"
                     /translation="ANSPGEFIVSATKIPVRDIASTVDGIAFAVQTATATEIRNSGMY
                     VTAVPSAAELNQVPGRVAVPGLAMHPTGALIYQPFLTGAAGAANVKGGVDISDARSGE
                     LRMRIILPQQLMTDVDALHGDFLTIDENGQRLFAITSLDGSPQSAALTVVQLASVPLA
                     IGSVSSPNVSVAGGTSLTIPGSGFQSGIKLGIGGKPAMATLVDMNTLNVVSPAVTAGP
                     QQLTLTNANGETVSLDAAVIAN"
BASE COUNT          387 a          530 c          429 g          389 t
ORIGIN      
        1 tcgatttcag cgatgtcctg cttggacagc gtcacatcgg cggcgcccac tacgccttcc
       61 acttgcttcg cgttccgcgc gccgacaatc acaccggtga tcgccgggtg tcgcaacacc
      121 cacgcgattg caatttcacc cggtgagcgg ccaagacgtt gcccaacctg tcgcagcctc
      181 tccaccagtt cgatattctt tgaaagtcgc ggctccttaa actcagcgcc acggctccga
      241 aaatcattcg cgggcagtgc cgccgcccgc tcgcgcgtca tcgcgccggt taataaaccg
      301 gaagccatag gagagtagga aatcacgccg attccttgtt tctcgcaata cggaaggatt
      361 tcagcttcaa tctgacgccg aatcaatgag tatggcggct gcagcgaagt gaccggagcg
      421 attttttcag cgcgtttcaa ttgctcgacg ttgaaattcg aaacgccgat ccagcggact
      481 ttgccttcct tctgcaacgt agccatcgtt tgccacgctt cttccaaccc cgcaccatta
      541 tccgccggcg gccaatgcat ctgatagaga tcgatcacgc tcacctgcag ccggcgcaaa
      601 ctggcttcgc attcgcggcg aatggaatca gcgctgtatt cattgcgaac tttgcgctgc
      661 tcatcccaaa ccatcgaaca tttcgtgaaa atgtagggcc gcgccccctt ccattctttg
      721 agggctttcg ccacaacttc ttcggaatga ccggtgccgt aaactgcagc cgtatctatc
      781 caattgacgc ccaattccaa cgctcgtttg atagccgcaa tggaatcgtt atcttcctga
      841 tttccccagc cgaattccca gccgcctccg ccgatggccc atgcgccaaa acccacgcga
      901 gttatattca ggtcggaatt accaagtttg cgagtctgca tcgtgtcgct ccaaactgaa
      961 tgagttgaga tagtcgttag tttggatgag gactagcggg aaaagttaga aactaattgg
     1021 caatcaccgc agcatccaat gaaacagtct cgccgttcgc gttcgtgagc gttagctgtt
     1081 gcggaccggc cgtcactgca ggcgaaacca cgttcagtgt attcatatcc accaaggttg
     1141 ccatcgctgg cttgccgcca attccgagct ttattcccga ttgaaacccg ctgcctggaa
     1201 tcgtcaacga tgtgcctccg gcaacagaga catttggtga cgaaacgcta ccgatggcta
     1261 gcggtacaga agccaattgc accaccgtga gggccgcgct ttgtggtgat ccgtccagcg
     1321 aagtgatggc aaacagccgt tggccatttt catcaatagt caaaaaatcg ccatgcaaag
     1381 catccacatc ggtcatcaat tgttgcggca aaattatccg catgcgcagt tcaccggaac
     1441 gcgcgtcaga aatatccacc ccgcccttta cattcgccgc acctgccgcc ccagttagaa
     1501 agggctggta gatgagcgcc ccggtcggat gcatcgccag tcccggcaca gcgacgcgcc
     1561 ccggcacttg atttagttct gcagcgctag gtacggctgt gacatacatt ccggaattac
     1621 gaatctcggt ggccgtggca gtctgcaccg caaacgcgat tccatcgacc gtactagcaa
     1681 tgtcccgcac gggaattttc gtcgcggaaa cgatgaactc tcccggcgag ttggc
//
LOCUS       DATCBB010000132         1644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_296296, whole genome shotgun sequence.
ACCESSION   DATCBB010000132 DATCBB010000000
VERSION     DATCBB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296296"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..586
                     /locus_tag="VII37_01530"
     CDS             218..586
                     /locus_tag="VII37_01530"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5068426.1"
                     /translation="MLQLSTSISEQRTDRRLAVRLPLTVSGRDSRGVAFREETSSENL
                     CRSGAAFVSRFDLPIGSDVEIHIPIAQSSVRRKEAEADFATRGRVVHVKSAETRGEKI
                     IGVRFTGPHFRRMFLSESAA"
     gene            complement(611..1567)
                     /gene="rsmI"
                     /locus_tag="VII37_01535"
     CDS             complement(611..1567)
                     /gene="rsmI"
                     /locus_tag="VII37_01535"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY5068427.1"
                     /translation="MSGKKFRRSSKADKLNKAEEAPANGEIPSAPASTAGCLYLVGTP
                     IGNLEDLTFRALRILKEVDQIACEDTRHTSKLLNHYQINKPLVSYHEHNEMTRAPELV
                     VALEQGARIALVSDAGMPLVSDPGHRLVAMCLRHHIQVVPIPGPSALLAALSASGLPN
                     EEFLFVGFLPSRSGERRRALERLRIENRTIIFYEAPHRVEECIADAREILGDRPACLA
                     REVTKLHEEFRRGKLSELTASLEARPARGEITLLIGPEDPAEARAHLDSTQSLSERVD
                     ELIRQAKLDRKDALKLAAKERGLTRRAAYGQLLEALGKTEVE"
BASE COUNT          327 a          403 c          526 g          388 t
ORIGIN      
        1 cgtttacttt ccgggtacta ccgacagatt agttctgcca atttacctga cgcggtgggc
       61 aaaatgaagc agttcgcatg ctggttcagc catggtgtgg ggaatggcag cgagctacgc
      121 cgtatcgtac atgcggcgcg aacgacccgc gagatattgg acagtgtgga gggtttcttc
      181 gcggcgcgct cgaaatcagc gggggaggac ccagtccatg ctccagctca gtacgtccat
      241 ttcggagcaa cgaaccgatc gtaggctcgc ggtgcgctta ccgctaacgg tgagcgggcg
      301 ggacagccgg ggcgtggctt ttcgagaaga aacttccagc gaaaatttgt gccgcagcgg
      361 tgcggcgttt gtctcgaggt tcgatctgcc catcggcagc gacgtggaaa tccatattcc
      421 cattgcgcaa agttcggttc ggcgcaaaga agcggaagcg gattttgcca cgcgcgggcg
      481 agttgttcat gtgaagagcg ctgaaacccg gggagagaaa attatcggtg tgcggtttac
      541 cgggccgcat ttccggcgga tgtttctttc agaatcggcg gcctaggcgg ggctgtttca
      601 ttccactttt ttattccact tcggtttttc ccaaagcttc aaggagttga ccgtaagcgg
      661 ctcggcgagt caagcctcgt tctttagcgg cgagctttaa ggcgtccttg cgatccagtt
      721 ttgcctggcg gatcaattcg tcgacgcgct cggataaact ttgcgttgaa tccagatggg
      781 cgcgcgcttc ggcgggatct tccggtccga ttaacaaagt aatctcgcct cgggcggggc
      841 gggcttccag agaagcggtg agttcggaga gtttgccgcg cctgaattct tcgtgaagtt
      901 tggtgacttc gcgggcgaga caggcggggc ggtctcccaa tatttcgcgg gcgtccgcaa
      961 tgcattcctc cacgcgatgg ggggcttcgt aaaatatgat ggtgcgattt tcgatgcgca
     1021 ggcgttccag ggcacggcga cgttcgccgc tgcgtgaggg caagaagccg acaaataaaa
     1081 attcttcgtt gggtaaaccg gaagctgaaa gagcggctaa cagtgccgag gggccgggaa
     1141 tgggcaccac ttgaatgtga tggcgcaaac acatggccac gaggcggtgg cctggatctg
     1201 agacgagggg cattccggca tcggagacca gagcgatgcg ggcgccttgt tcgagggcga
     1261 caaccagctc gggtgcgcga gtcatttcgt tgtgctcgtg atagctgact agcggcttat
     1321 tgatttggta gtggttcagt agcttggaag tgtggcgggt atcttcgcag gcgatctgat
     1381 caacttcttt taggattcgg agagcacgga aggtcaggtc ttcgagattg ccgatgggcg
     1441 taccgaccaa ataaaggcaa ccggcggtgc ttgcaggcgc gctaggtatt tcgccgttcg
     1501 cgggtgcttc ttccgcttta ttcagtttgt ctgctttgga agatcgacgg aatttcttac
     1561 cgctcatgac ttcgagtcta ccacgcgctc cggaagctgg ctgtcacgca tgttataatc
     1621 agatttctac ttcagtaagg agac
//
LOCUS       DATCBB010000133         1817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_296320, whole genome shotgun sequence.
ACCESSION   DATCBB010000133 DATCBB010000000
VERSION     DATCBB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296320"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VII37_01540"
     CDS             complement(<1..1455)
                     /locus_tag="VII37_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY5068428.1"
                     /translation="MASEETGKSRSRAWIVVLCVVLLVFIIPAVILYQRMRDVDDVTR
                     DWVVRALSERFASRVELESIHVTAFPEMSVTGEKLAIYYHNRTDVPPMIQIQQFTFRL
                     GFMGILAVPRHIRGVHLDNMVITVPPRGQPLGEPAPPPPTKIKKPLPRITIDQVVCDN
                     TTLLILPKQSGKQPLDFDIHDLVLSSVGAGKPFSFRGNLTNAKPVGEIRTNGSFGPWQ
                     VDDPGGTAVSGSYTFKDADLGPLPGIGGILSSSGKYSGVLQSIDVEGVTDTPDFSIDP
                     VGRAVPLRTEFSATVDGTNGDTYLHPVRATLIHSQITAKGSVVRVPEQHGHLILLDVV
                     APKARLEDLLQLATKSTQPMMSGLVNLRTKMVLPPGKVKVLDKLTLDGEFDVSDARFA
                     SSDVRNKLASFSRHALGQPDDPDAGSAISDLRGKFNLKNGVIKFDRLTFSIPGTVIKL
                     NGTYKLRGEDLDFTGELRMEAKLSQLVTGKKSLFL"
     gene            complement(1481..>1817)
                     /locus_tag="VII37_01545"
     CDS             complement(1481..>1817)
                     /locus_tag="VII37_01545"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY5068429.1"
                     /translation="ADYLIATDLRSEVPVGFKAGGVSYPKGELHTFSFSKTPLNVYQG
                     TVTLRMPITALANAPVGPQQIPLKLRYQACSTEICLPPVTIDLQATINIVASAAAAKP
                     AHAEIFPAP"
BASE COUNT          428 a          547 c          441 g          401 t
ORIGIN      
        1 cagaaaaagc gactttttgc cagtcaccaa ttgcgaaagc ttcgcctcca tgcgcaactc
       61 cccggtgaaa tccagatcct cgccgcgcaa tttgtaagtc ccattcaact tgatcactgt
      121 gcccggaata ctaaaagtca atcgatcgaa tttgatcact ccatttttca gattaaattt
      181 tcctcgcaaa tccgaaatcg cgctaccagc gtcaggatcg tccggttgac ctagggcatg
      241 gcggctgaaa gacgccagct tattgcgcac atcggagctg gcaaagcgcg catcactaac
      301 atcaaactcc ccgtccagcg tcagtttatc cagcactttc actttgccgg gcggcaaaac
      361 catttttgtt cttaaattaa ccaacccgct catcatcggc tgcgtcgact tcgtagccaa
      421 ctgcagcaag tcctcaagtc gcgctttggg tgccacgaca tcaagcaata tcaagtgccc
      481 gtgctgctcc ggcacgcgca ctacgctccc ttttgcggta atttgcgagt ggatcagcgt
      541 tgcgcggact ggatgcaaat acgtatcacc gttggtgccg tccaccgtgg cggaaaattc
      601 cgtgcgcagc ggcaccgcgc gtcccaccgg atcaatcgaa aaatccggcg tgtcggtaac
      661 accttccacg tcgatactct gcagcacccc cgaatatttg ccgctcgaag acaatatccc
      721 gccaataccg ggcagcggcc cgaggtccgc atccttgaac gtatacgaac ccgaaaccgc
      781 cgtgcctccc gggtcgtcca cttgccacgg cccaaagctt ccgttcgttc ggatttcccc
      841 gacgggcttc gcatttgtca ggttgccgcg aaatgaaaat ggtttgcccg cgcccacaga
      901 gctcaacact aaatcgtgaa tatcaaaatc aagcggctgc ttgccgctct gtttcggaag
      961 aatgagcagt gtggtgttat cgcacaccac ctgatcaatc gtaattcttg gaagcggctt
     1021 ttttatttta gttggcggcg gtggtgccgg ctcgcccagt ggctgcccgc gcggtggaac
     1081 agtgatcacc atattatcca ggtgcacgcc ccgaatatgc cgcggaacgg ccaaaatccc
     1141 cataaatccc agacgaaacg tgaattgttg gatctgaatc atcggcggaa cgtcagtgcg
     1201 gttgtgataa tagatcgcca gcttctcgcc ggtaacgctc atctccggaa aagccgtcac
     1261 atgaatggat tccagttcca cccggctcgc gaatcgttcc gaaagcgcgc gtaccaccca
     1321 gtcgcgcgtt acgtcatcca cgtctcgcat tcgctgatac aagatcaccg caggaataat
     1381 gaacaccagc agaacaacgc acagcaccac aatccacgcg cgtgaacgcg attttccggt
     1441 ctcttcggac gccatctcgg gagaataccg cagtgtagct ttacggtgct gggaatattt
     1501 ccgcgtgtgc cggcttggcg gcagcagcgc tggccacaat atttattgtc gcctgtaaat
     1561 caatggtcac aggcggcaaa cagatttccg tgctgcatgc ttgataccga agcttcagcg
     1621 ggatctgctg cggtcccacc ggcgcgttcg ccaatgcggt gatcggcata cgcaaagtta
     1681 ccgttccctg gtaaacgttg agcggagtct tcgaaaagga aaaagtatgc agctcacctt
     1741 tcggatatga cacaccaccc gctttaaacc caacaggtac ctccgagcgc aaatccgtgg
     1801 cgatcaaata atccgcc
//
LOCUS       DATCBB010000134         2409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_296392, whole genome shotgun sequence.
ACCESSION   DATCBB010000134 DATCBB010000000
VERSION     DATCBB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296392"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..688)
                     /locus_tag="VII37_01550"
     CDS             complement(122..688)
                     /locus_tag="VII37_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY5068430.1"
                     /translation="MRKTAVLTGVLFLGTALSGVPLISQAAGAHKLQQNEPKSRSNLI
                     REVRHQLVLLPYYSVFDNLAFKVEGDTVILEGQVVRPTLKSDAEGVVKNIEGIGRVQN
                     NIEVLPASPMDDQIRRAVYRTIFSEPGLSRYAESAVPSIHIIVKNGHVTLVGVVDSEG
                     DKNLVNIRTNGVPNVFSVKNELVVTPSK"
     gene            886..1617
                     /locus_tag="VII37_01555"
     CDS             886..1617
                     /locus_tag="VII37_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY5068431.1"
                     /translation="MLLIAHRGASGHAPENTLASFKKAVTLGATFIETDLQLTRDARL
                     VAIHDDTVNRTTNGKGAVHDMTLADLRRLDAGSWFGSEFAGERIPTLDEVLDFAKKYD
                     VIFYLEMKPGGSWGGEHALIGALRLSGEIARTIVISFDPAILANVRKIEPTVMTALLY
                     DGHLEKPLERALEVGARQLAIRGDLVSPSLLAAAREKDLQVVCWTINHSAHMRVLMAA
                     GVDGIMSDYPDRLAATAKTANVKNS"
     gene            complement(1662..>2409)
                     /gene="rsmA"
                     /locus_tag="VII37_01560"
     CDS             complement(1662..>2409)
                     /gene="rsmA"
                     /locus_tag="VII37_01560"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY5068432.1"
                     /translation="ADYCWVEIGPGHGEMTEYLVASGVPVHVVEMDQFLIGNLNRLKK
                     KNSNLEVICGDILETDLAAVAHGRRMRIYGSLPYYITSPILHHLFQFAELIDEIHVVV
                     QTEVAERMAAEPGSKAYGYLSVATQLNSRPELVLEIPRAAFSPPPEVGSTLVTLRLPG
                     GWEQLRSGGLLEGENKSAQDKTEGEENFLEFVKLCFSKKRKTLVNNLRELASPEAVRN
                     ALGDVGLRPDARAEQISISNFAALYTALRK"
BASE COUNT          524 a          730 c          583 g          572 t
ORIGIN      
        1 ataacgtgag acattccatg gccgaaagta acagtgtgag cagcgcgccg cccggcgccc
       61 taaaagaaaa cggctcgcac cggcgagccg tgatcctaat tgatatgcag atcggttggt
      121 tttacttgga cggcgtcacc accaactcat tttttaccga gaaaacgttc ggtacgccgt
      181 tcgtccgtat gttgacgagg ttcttgtctc cctcgctatc taccactccg accaaagtga
      241 catgtccgtt tttcacgata atgtgaattg acggtaccgc ggattcagca tatcgcgata
      301 gtcccggctc gctaaaaatc gtacggtaca ccgctctccg gatctggtca tccatcggcg
      361 atgcgggcag cacctcgatg ttgttctgga ccctgccgat tccttcaatg ttttttacca
      421 ctccttcggc atccgacttc agcgttggcc gaaccacctg cccttccagg attaccgtgt
      481 caccttccac cttgaaggcg aggttatcga acaccgagta gtagggcagc aacaccagtt
      541 ggtggcgcac ttcgcggatc agattcgacc gcgattttgg ttcattttgt tgcaatttgt
      601 gggcacctgc tgcctgcgaa atcaagggca cgccgctgag tgccgttccg aggaataaca
      661 ccccagttaa aacagccgtt ttcctcataa tagtctccag ttctatttat tctcttaaca
      721 cgatagttag attcaagttg cgtgccaatg ggtgcgcgtg gcgctgcagg cggccgatgt
      781 tacgatagag ccagtggatc catcttttct cacgttgtcc atgaagccct ggctcttcta
      841 cttcggcatt ttgcctacta ttcattcttc tgcgagtgcc tggccgtgct gttgatcgcc
      901 catcgcgggg cctccggtca cgcgcccgaa aataccctgg cctcgttcaa gaaagcggta
      961 accttaggcg ccacatttat cgaaaccgat ctgcaattga ctcgcgacgc gcgcctggtg
     1021 gctattcacg atgacactgt gaatcgcacc accaatggaa agggcgctgt tcacgacatg
     1081 accctcgccg atttgcgccg tctggatgcc ggttcctggt tcggcagcga attcgctggc
     1141 gaacgcattc ccaccctcga tgaagttctc gattttgcca agaaatacga cgtgatcttc
     1201 tacttagaaa tgaagcccgg cggctcctgg ggcggcgagc acgcactcat cggcgccttg
     1261 cgattgtctg gggaaatcgc gcggaccatc gttatttcct ttgaccccgc gattctagcc
     1321 aacgtccgaa aaattgaacc aaccgtgatg accgctttgc tctacgacgg ccaccttgaa
     1381 aaacccctcg agcgcgccct cgaagtgggg gcgcggcagt tggccatccg cggtgatctc
     1441 gtgagtcctt cgctgctcgc cgcggcgcga gaaaaagatt tgcaagtcgt atgctggacc
     1501 ataaatcact ccgcgcacat gcgcgtgctt atggctgcgg gcgtcgacgg catcatgagc
     1561 gattatcccg accgcctggc ggccaccgca aaaactgcca atgtaaaaaa ctcctagcag
     1621 tcgcgtgctt ctctgcttgc cgccgtgctt tttgaacggt tttacttcct caacgccgta
     1681 tacaacgccg caaaattgga aatcgaaatc tgctcagcac gcgcatccgg gcgcaggccg
     1741 acgtccccta gcgcattgcg caccgcttct ggcgacgcca actcccgcaa attattaacc
     1801 aatgtttttc gcttcttcga aaaacacaac ttaacaaact ccagaaaatt ctcttcgcct
     1861 tccgtcttgt cctgcgcgga tttattttct ccctctaaca agccgccgct tcgcaactgc
     1921 tcccatccac caggcaaccg cagcgtcacc aaggtcgacc caacttcagg cggtggtgaa
     1981 aaggccgccc gtggaatctc aagcaccaat tccggccgcg aattcaattg cgtcgccacc
     2041 gaaagatatc cgtaggcttt actgccaggc tccgccgcca tgcgctctgc gacttccgtt
     2101 tgcactacga catgaatctc gtcaattaat tccgcaaact gaaacaaatg gtggaggatc
     2161 ggcgaagtga tgtaatacgg cagactccca taaatccgca tgcgtctccc gtgggcaaca
     2221 gccgccaggt cggtctcaag aatgtctccg caaatcacct cgagattcga atttttcttc
     2281 tttagtcggt tgagattccc gattaaaaat tggtccatct cgacgacatg caccggcacg
     2341 ccactggcca cgaggtattc ggtcatctcc ccgtggccgg ggccaatctc aacccagcaa
     2401 taatccgct
//
LOCUS       DATCBB010000135         2114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_458482, whole genome shotgun sequence.
ACCESSION   DATCBB010000135 DATCBB010000000
VERSION     DATCBB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458482"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VII37_01565"
     CDS             complement(<1..468)
                     /locus_tag="VII37_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY5068433.1"
                     /translation="MKYAIFGATGTVGKALAAELAKRGSSFRVVGRSNETLRRHFHAY
                     EPLVEYCEADLSDPLAAARAAAGVDTIFYTVGVPYTQFALHPKLIKIALEAATSAHVS
                     RFIHVSTVYPYGIPETDFVNESHPRNPIAFKGKMRKEQEDLVFAADKPGGMRTT"
     gene            651..1181
                     /locus_tag="VII37_01570"
     CDS             651..1181
                     /locus_tag="VII37_01570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5068434.1"
                     /translation="MSRDAGTYAIFETSQGDIVIRLLEKEAPKTVENFIGLAEGTKEF
                     TNEKTGKKEKRNFYDGLNFHRVIPQFMIQGGCPEGTGRGGPGYKFADEFHPSLKHSKA
                     GKLSMANAGPGTNGSQFFITVAATPWLDNKHTIFGEVVEGQEVADKISNAPRDSGDRP
                     KTPITLKKVRIERVAA"
     gene            1240..1614
                     /locus_tag="VII37_01575"
     CDS             1240..1614
                     /locus_tag="VII37_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068435.1"
                     /translation="MLTPERLLRVVIEIIFVLLGLLVVWLGLTGHIFFDRHKLPWLVL
                     SVALILWGIRGIYRKPGSKPPQPEDYIRAVSLTALGIVLLAISRVPFAWVGPLLACAG
                     VVLAARGIAGSVLLLRPAGARR"
     gene            1636..2070
                     /gene="mscL"
                     /locus_tag="VII37_01580"
     CDS             1636..2070
                     /gene="mscL"
                     /locus_tag="VII37_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY5068436.1"
                     /translation="MFKEFKTFAMRGNVIDLAVGVIIGLAFGKVVSSLVDDIIMPPIG
                     KLLGHIDFSNLFVSLNGMHYDTLAAAKAAAAPTINYGLFLNTIINFLIVAFAVFLLVQ
                     QVNRWGKKSAPAAAPTTRDCPQCAMPIPLAAKRCPHCTSQLA"
BASE COUNT          448 a          478 c          672 g          516 t
ORIGIN      
        1 ggtggtgcgc attccgccgg gtttgtcggc tgcaaagacc aaatcttcct gttccttgcg
       61 catcttgcct ttgaaggcga ttggatttct cggatgggat tcgttgacaa agtctgtctc
      121 tggaatgccg tagggatata cggtgctgac atggatgaaa cgcgaaacgt gcgctgaggt
      181 agcggcttcg agtgcgatct tgatcagttt cggatgcagc gcgaattgtg tgtaggggac
      241 tcccacggta taaaaaatcg tgtccacacc cgccgcggcc cgagcagcag ctaacgggtc
      301 tgaaagatcg gcttcacagt attcgacgag cggttcgtag gcgtggaaat ggcggcgcag
      361 ggtctcgtta gaacgcccga ctactcggaa actggaacct ctttttgcta gctctgccgc
      421 cagagccttc cctacggttc ctgtggctcc aaaaattgcg tatttcaacg gtcgttaccc
      481 ttcctctgat ctttgcgtac gggaggaact gatggcctaa tagatgcgcg acgattgtct
      541 ttgttataag cggatgtgaa gactgccagc gctttacagg gtgtgctggt tgggtggcgg
      601 cggcggcgaa tggtcgtgta cgataaatac gaacttggga gaggaaacta atgagcagag
      661 acgcgggaac gtacgcaatt tttgagactt cgcagggcga tattgtgatt cgtttgctgg
      721 aaaaagaagc gccgaagacg gtggagaatt ttatcgggct cgcggaaggc accaaagaat
      781 ttacgaatga gaagactggg aagaaggaaa aacgaaattt ttatgatggc ctgaattttc
      841 accgggtgat tccgcaattc atgattcagg gaggatgccc ggagggaacc gggcgcggtg
      901 ggccgggtta caaattcgca gacgagttcc atccttcgct gaagcattcg aaagcgggca
      961 agctgtcgat ggccaatgcc gggccgggga ctaatggcag ccaatttttt attaccgtgg
     1021 cggcgacgcc gtggctagac aacaagcaca cgatattcgg tgaggtggtg gaagggcagg
     1081 aagtcgcgga caagatttcc aacgcgccgc gcgattctgg cgaccggccg aaaactccga
     1141 ttactttgaa gaaggtgcgg atcgagcggg tggcggcgta gcgctattgt ttgtaccgcg
     1201 cttgtcgggc ctgacgtttt tttcatgtga taaaccgcga tgctcactcc tgagcggcta
     1261 cttcgagtag tgatcgaaat tatttttgtg ttgctggggc tgctggtggt gtggctgggg
     1321 ctcaccgggc acattttttt tgaccggcac aaattgccat ggctagtgct gagtgtggcg
     1381 ttgatcctgt ggggtattcg cggaatttat agaaaaccgg gcagcaagcc gccacagccg
     1441 gaagattata tccgcgcggt ttcgctgacg gctttgggta tagtactgct ggcgatttcg
     1501 cgagtgccgt ttgcatgggt ggggccattg ctggcgtgcg cgggggtggt gctggcggcg
     1561 cgaggcatcg caggatccgt gttattgttg cggcccgctg gtgctcggcg gtgaggcagc
     1621 gggaaaggaa ctgacatgtt caaagaattc aagacttttg cgatgcgcgg gaatgtgatc
     1681 gatcttgcgg tgggcgtgat catcggccta gctttcggaa aagttgtgtc ctcgttggtg
     1741 gatgacatca tcatgccgcc aattggcaaa ttgctgggac atattgattt ttcgaacttg
     1801 tttgtgagcc tgaacgggat gcattacgac acactcgcgg cggcgaaagc ggcggcggca
     1861 cccacgatta attacggatt gtttttgaat acgatcatta actttttgat cgtggcgttc
     1921 gcggtgtttt tgctagtgca gcaagtgaat cggtggggga agaaatcggc gccagcagcg
     1981 gcaccgacga ctcgcgactg tccacagtgc gcgatgccaa ttccattggc ggctaagcgt
     2041 tgtccgcact gcacgtcgca gctcgcctag cggctaactc gcgcgaggtt caacttcaac
     2101 ttagcgtaac gttt
//
LOCUS       DATCBB010000136         4764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_166551, whole genome shotgun sequence.
ACCESSION   DATCBB010000136 DATCBB010000000
VERSION     DATCBB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166551"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1425)
                     /gene="murA"
                     /locus_tag="VII37_01585"
     CDS             complement(127..1425)
                     /gene="murA"
                     /locus_tag="VII37_01585"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5068437.1"
                     /translation="MDKFLIKGGKPLHGTVAISGAKNSALPAMAAALLTKEPVTLHNI
                     PKVRDLITMSKLLTFMGAKVSITDIPASDYVIQAVKLSDAVAPYELVKTMRASILTLG
                     PLMARMGVARVSLPGGCAIGARPVDLHLAALEKMGAEITTDHGYIQAKVPGNGNDGHN
                     GRLKGANITFEKITVTGTENILMAAVLAEGETVLNNAAREPEVTDLVDMLRKMGAQIE
                     GDCSSTLRIRGVEKLRGTEHAVIPDRIEAGTFLVAGAITDGDLTLTNCAPEHLVSIIA
                     KLEQTGVRIEALDKTTLRVRGAKKLIAADVTTEEYPGFATDMQAQYMALATQAAGASV
                     ITETIFENRYLHASEMLRMGANIAIDGRRAVVRGPSPLTGTTVIASDLRASASLVLAG
                     LVASGETTIDRVYHIDRGYEDIVKKLRGVGAQIVRLHIKD"
     gene            complement(1514..2416)
                     /gene="murQ"
                     /locus_tag="VII37_01590"
     CDS             complement(1514..2416)
                     /gene="murQ"
                     /locus_tag="VII37_01590"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEY5068438.1"
                     /translation="MPPKPRENRRSELRNSASKNLDRLSALQIVRLMNREDRKVAVAV
                     GRTLPAIARAVDLIVEAVRGGGRLIYVGAGSSGRIAVLDAAECPPTFGTSPQLVQTLI
                     AGGGRAITRAVEGAEDSPGNAERDLRKIKLTRNDVVAGICASGTTPYVLGALRFASKT
                     GAATIAITVNRNTPVARLPDVLIATEVGPEILTGSTRLKSGTSQKMVLNMLTTAAMAR
                     LGHVYENLMIDVKASNRKVSQRMVRILADASGKGLSASEHALRQAGHNLRVALVMLKL
                     QINARDAARKLRDAKGHLRAALGE"
     gene            2613..4040
                     /locus_tag="VII37_01595"
     CDS             2613..4040
                     /locus_tag="VII37_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY5068439.1"
                     /translation="MNILNRLFEERFRSPVTHVHPLQGQLGGSGRQIIRLSNEANTAI
                     GIVYGVREENVAFLEFSKHFRKHGLPVPEIYGEDLDHGVYLEEDLGNATLYEFVSKNR
                     DAATIAPPVVEAYRKVVALLPRFQVEAGHDVNYEVCYPIGSFDRQSVAWDLNYFKYYF
                     LRLAGVPFSEQALEDDFGRLTDFLMTADRDYFLYRDFQSRNIVLRNGQPYFVDYQGGR
                     KGALQYDIASLLFDAKADLPPELRQELLNHYLDALSGYIKLDREAFMKHYYAYVYVRI
                     LQALGAYGFRGFYERKQHFLQSVPFALKNLRWLLHHVKLPIELPTLMAAFQSMLASEK
                     LQGLAGEAEHLKLRIFSFSFHQGAPKDETGHGGGFVFDGRSLPNPGREERFKPLTGKD
                     TPVIEYLNQQESVHQFLASVMSLVDASVASYNERGFKNLMVSFGCTGGQHRSVYLAEQ
                     LAKHFRARNGVEVAVRHLQLEKMGK"
     gene            4125..>4764
                     /locus_tag="VII37_01600"
     CDS             4125..>4764
                     /locus_tag="VII37_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5068440.1"
                     /translation="MKAMVLAAGLGTRLRPLTDDRPKALVQVAGRTLLELTLARLRAF
                     GIHDVIVNVYHFADKVTSFLKEHENFGMHIEVSREDMLLDTGGGLKKAGWFFLEQSDL
                     AQEPFILHNVDVVSTIDFQRMVQFHKDNQALATLAVQHRETSRYLLFDEQFQLCGRRV
                     GREGNPQIVRAAPKMEALAFSGIHVISPRFLTMMQEEGVFSIIDTYLRLAGSG"
BASE COUNT         1013 a         1357 c         1305 g         1089 t
ORIGIN      
        1 atatggattt gcgttgtggt tgcgctttat ccactctgca aatggtatgc agggttgagg
       61 cagcggagca gcaatccgtg gctcagctat ctctgaagcc gcactcgcca tatcgcgaaa
      121 ttgaaatcaa tctttgatgt gtaggcgcac aatttgcgcg ccgactccgc gcaatttttt
      181 gacaatgtct tcgtagcctc ggtcgatgtg ataaacgcga tcgatagtag tctcgccact
      241 ggccaccaat ccggcgagca ccagggaagc gctggcgcgc agatcgctgg cgattacggt
      301 agtaccggtc aacggcgaag gcccgcggac caccgcgcgg cggccatcaa tagctatatt
      361 tgcgcccatg cgcagcattt cgctggcgtg aaggtaacga ttttcgaaaa ttgtttcggt
      421 gattacggag gcgcctgccg cctgggtagc cagagccatg tattgcgcct gcatgtccgt
      481 tgcgaagccc ggatattcct cagtggtgac atcggcggcg ataagtttct ttgcgcctcg
      541 cactcgcaag gtggttttgt ccaaggcctc gatgcgcact ccggtttgct ccaatttggc
      601 gatgatggag actagatgtt ccggcgcaca attggtcagc gttaaatcac catcggtgat
      661 ggctccggcc acgagaaagg tacccgcctc gatgcggtcg ggaatcacgg catgttcggt
      721 gccgcgcagt ttttcaactc cgcgaatgcg cagcgttgag ctgcaatcgc cttcgatttg
      781 cgcgcccatt tttcgcagca tgtccaccag gtcggtcact tccggttcac gtgccgcgtt
      841 gtttagaacc gtttctccct cggcaagcac cgcagccatc aaaatatttt cggtgccggt
      901 gacggtaatt ttttcaaagg tgatatttgc gcctttgagc cggccattgt gcccatcgtt
      961 gccgttgccg ggaactttgg cctgaatgta gccgtgatcc gtggtgatct ccgcgcccat
     1021 tttttcaagc gccgcaagat gcaggtccac cggacgcgcg ccaatggcgc agccgccggg
     1081 aagcgaaacg cgcgccacgc ccatgcgtgc catcagcgga cccagcgtaa gaatcgacgc
     1141 acgcatagtc ttcacgagtt cgtacggcgc gaccgcatcg cttaacttga cggcttggat
     1201 gacgtaatcg ctggcaggaa tatccgtaat ggaaactttg gcgcccatga acgtcagcaa
     1261 cttgctcatg gtgatgaggt cgcggacctt gggaatattg tggagagtga cgggttcctt
     1321 cgtcagtagc gcggccgcca tcgccggcag cgcggaattt ttcgcgccac tgatggccac
     1381 cgttccgtga agtggcttac cgcccttgat tagaaattta tccatgagag ggaatcaacc
     1441 aaaatttgaa atgcgaaccg ttagctcgcg tatgtggtcc aagtcgagca cgcacgtgta
     1501 ttgcgcttca cggctactca cccagtgctg cacgcaaatg tcctttcgca tcgcgcaatt
     1561 tccgagctgc gtccctagcg tttatctgca acttcagcat caccagcgcc acgcgcaagt
     1621 tgtgtccggc ttgacgcaaa gcgtgttccg aagccgacag gcccttgccg ctagcgtccg
     1681 ccaggatgcg caccatacgt tgcgaaacct tgcgattcga tgctttcaca tcgatcatca
     1741 agttttcata aacgtgtccc agacgagcca tcgctgctgt agtcagcatg ttgagaacca
     1801 ttttctgcga agtccctgat ttcagacgcg tcgacccagt gagtatctct ggccccacct
     1861 cggtagcgat gagcacgtcc ggcaagcgcg ccaccggggt gttgcgattc acggtaatcg
     1921 cgatagtcgc ggcgccagtc ttcgacgcga agcgcagcgc acccagcaca tacggggtag
     1981 tgccgctggc gcaaatcccc gcgaccacat cattccgcgt caattttatt tttcgcagat
     2041 cgcgctcggc gtttcccggt gaatcctcgg cgccttcgac tgcgcgcgta atcgcgcggc
     2101 cgcccccggc aatcaatgtc tgcaccagtt gcggcgacgt tccaaatgtc ggcgggcact
     2161 ccgcggcatc cagcaccgcg atgcgtccac tggaaccagc acccacgtag atgagccgcc
     2221 ccccgccgcg aacggcttcc acgatcaggt caaccgcgcg cgcgatagcg ggaagtgtcc
     2281 ggcccaccgc tacggccact ttgcggtctt cccgattcat cagccgcacg atttgtaacg
     2341 cgcttaaacg gtcgagattt ttcgacgctg aatttcgcag ctcactgcgt ctattttccc
     2401 tgggcttcgg aggcacagtc gcattatagc tttgggctgc cgcaatccgg gcgcacgcaa
     2461 tgaaaaacgc cgctgccttg ccaacaaatt gctaataact tcaggaaact agacctttcc
     2521 tatttgcgga gaccaagtag cgattcggct atcatgaaac gacggactgg caacaggttg
     2581 tcaacagggc ggactccttg gaaatcgcac taatgaacat tctgaaccgg ctgtttgagg
     2641 agcgattccg ttcgcccgtc acgcatgtgc atccgctaca aggccaactc ggcggctctg
     2701 ggcgccagat catccggctt tccaacgaag cgaacaccgc catcggcatt gtgtacggcg
     2761 tacgcgaaga aaatgtcgca tttctcgaat tttccaaaca tttccgcaaa catggcttgc
     2821 cggttcccga aatttacggc gaagacctcg accacggcgt atatctggaa gaagacctcg
     2881 gcaatgccac gctgtatgag tttgtatcga aaaaccgtga tgccgcgacg attgccccac
     2941 cggtggtgga agcctatcga aaagttgtgg ccctcttgcc ccgctttcaa gtcgaagctg
     3001 gtcacgacgt gaattatgaa gtgtgttatc ccatcggcag ttttgatcgt cagtccgtcg
     3061 cctgggattt gaactacttc aaatactatt ttctccgtct cgccggcgtt cccttcagcg
     3121 agcaagcctt ggaagacgat ttcgggcgct tgaccgattt cctgatgacc gcggaccgcg
     3181 actatttcct gtaccgcgac tttcaatcga gaaacatcgt gctgcgtaac ggccaaccgt
     3241 attttgtgga ctaccagggc gggcgcaagg gcgccttgca gtatgacatc gcgtctctct
     3301 tgttcgacgc caaggctgac ttgccgcccg agctgcggca ggaacttctc aatcattatc
     3361 tggatgctct aagcggttac ataaaactcg atcgcgaagc gttcatgaag cattactacg
     3421 cctacgtgta cgtgcgcatt ctgcaggcgc tgggagcgta cggatttcgc ggattttatg
     3481 aacgcaaaca gcattttttg caaagcgtgc cgtttgccct gaagaacctg cgctggctct
     3541 tgcatcacgt caagctgccc atcgagctgc ccacattgat ggccgcgttc caaagcatgc
     3601 tggcctcgga gaaattgcag ggattggccg gcgaagcgga acatctcaaa ctgcgcattt
     3661 ttagtttctc ttttcatcag ggagcgccca aggatgaaac cggccacggc ggcggatttg
     3721 ttttcgatgg acgaagtctg ccgaatcccg gacgtgaaga gcgcttcaaa ccgctcactg
     3781 gcaaggacac tccggtgatc gaatatctca accagcagga aagcgttcat cagtttctgg
     3841 ccagcgtgat gtctttagtc gatgccagcg tcgccagtta caacgagcga ggattcaaaa
     3901 acctcatggt ttctttcggc tgcaccggtg ggcagcaccg ctccgtctat cttgcggagc
     3961 aattggcgaa acattttcgt gctagaaatg gcgtggaagt ggcggtgcgg catctgcaac
     4021 tcgaaaagat gggcaaatga atgtgcccgc gatgcagggc ctcgtgatcg tggcctcccc
     4081 tatgcaaagt catttgatga aagcctctca atgaaagccc gccaatgaaa gcgatggtgc
     4141 ttgctgccgg gctgggtaca cggttgaggc cgctcaccga tgaccgtcca aaagcgctag
     4201 tgcaggtcgc ggggagaaca ctgctggaac tgacgctggc tcgcctgcgc gccttcggga
     4261 ttcacgacgt gatcgtaaac gtgtaccact tcgcagacaa agtgacttcg tttctgaagg
     4321 agcatgaaaa tttcggcatg cacattgaag tgtcgcgcga agacatgttg ctcgataccg
     4381 gcggcggcct aaaaaaggcc ggctggtttt ttcttgagca gtctgactta gcccaggaac
     4441 cgttcattct ccataacgta gacgtggtga gcacgattga ctttcagcgc atggtgcagt
     4501 ttcacaaaga caatcaagcg ctcgccacac ttgccgtgca gcaccgtgaa accagccgct
     4561 acctgctatt tgacgagcag tttcagctct gcgggcggcg agttgggcgc gaagggaatc
     4621 cacagattgt gcgagccgcg ccaaagatgg aggccttggc gttctccggc attcacgtca
     4681 tttccccacg cttcttaacg atgatgcagg aagaaggtgt tttctccatc atcgatactt
     4741 atctacgcct ggccggaagc ggag
//
LOCUS       DATCBB010000137         1077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_231142, whole genome shotgun sequence.
ACCESSION   DATCBB010000137 DATCBB010000000
VERSION     DATCBB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231142"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VII37_01605"
     CDS             <1..584
                     /locus_tag="VII37_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068441.1"
                     /translation="LFLSAAALSCAILFSSLTVIARQQDNSQQTGDPVADAARRAREA
                     KQNAPKPRKVYTDDDVSRPKPAPAAATATSTTPDSTAQPGGTSANTPAPADQDAKTEA
                     AWRQRFKEQRDKISRAEKELDILQRETQKAEIQYYPDPQKAMNEQYTRKEINDKDAKI
                     EAKRKEIADLKQGLSDLEDALRKAGGDSGWSRE"
     gene            617..763
                     /locus_tag="VII37_01610"
     CDS             617..763
                     /locus_tag="VII37_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068442.1"
                     /translation="MATESKLDEAADILAKIVKEQIKSLPPAVVVSKRKTLLELATSF
                     QRSR"
     gene            complement(770..>1077)
                     /locus_tag="VII37_01615"
     CDS             complement(770..>1077)
                     /locus_tag="VII37_01615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5068443.1"
                     /translation="EEGLREMMRVLKPGGTLAILEFTEPPEGLLGNLYRWYFRGVLPR
                     IGGMLSGDHDAYTYLPRSVSRFFRPAQLKALMESVGYTTVQYRVWTMGTVAFHTAQR"
BASE COUNT          309 a          343 c          247 g          178 t
ORIGIN      
        1 aattatttct ctcagcagca gcgctaagct gcgccattct gttctcttcg ctcacggtga
       61 tcgcgcgcca gcaagacaac tcgcagcaaa ccggcgaccc cgtcgccgac gccgcccgcc
      121 gcgctcgcga agccaagcaa aacgccccga aacctagaaa agtttatacc gacgacgacg
      181 taagcaggcc caagccagca ccagcggcag ctactgccac ctccacaacg ccggattcca
      241 ccgcgcaacc tggtggcact tccgcgaata ctccagctcc cgcagatcag gacgccaaaa
      301 ccgaagccgc ctggcgccag cgcttcaaag aacaacgcga caaaatttcc cgcgcggaaa
      361 aagaactcga catcctgcaa cgcgaaacgc aaaaagccga aattcagtat tatcccgacc
      421 cgcaaaaagc catgaacgag cagtacaccc gcaaagagat caacgacaag gacgccaaaa
      481 tcgaagctaa gcgcaaagaa atcgcagatt taaaacaagg tttgagcgac ttggaagacg
      541 ccttgcgcaa agccggcggc gactccggct ggtcccgcga atagcgcgtt cgcgttttcg
      601 gtattttgtc aatccaatgg caacagaatc aaagctggac gaagccgcgg atatcctggc
      661 gaaaatcgtc aaagagcaaa ttaaaagctt gcctccagcg gtagttgtat cgaaacgcaa
      721 aacgctgctt gagttggcga caagctttca gcgatcgcgg tgaacgtaat caccgctgcg
      781 cggtgtgaaa agccaccgta cccatcgtcc acactcgata ctgcaccgtc gtataaccca
      841 ccgactccat cagcgctttc agttgtgcag gccgaaagaa tcgtgacacc gatctcggca
      901 gatacgtata cgcatcgtga tcacccgaaa gcatcccacc aatccgcggc agcacgccgc
      961 gaaaatacca tcgatataaa ttgccaagaa gcccttccgg cggctcggta aattcaagaa
     1021 tcgccaaagt cccacccggt ttcaaaacgc gcatcatctc tcgcaaacct tcttcat
//
LOCUS       DATCBB010000138         1204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_393591, whole genome shotgun sequence.
ACCESSION   DATCBB010000138 DATCBB010000000
VERSION     DATCBB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393591"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VII37_01620"
     CDS             complement(<1..1197)
                     /locus_tag="VII37_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5068444.1"
                     /translation="MSDPLTGLPNRLLFIDRLNRLIKYSKRRKDHSFAVVFMDLDGFK
                     MINDSMGHMVGDQLLIGVADRLEKCLRASDTLARLRDTYTVARLGGDEFTVLLDDLKD
                     PNDAKIAADRLMKALKPPFLLAGKEVYTSVSIGIAQSNPVYDGPDEMLRDADTAMYHA
                     KSLGKGRYEVFDAKMRASVMARLQLETDLRHALERGEFRNFYQPIVNLASGEIAGFEA
                     LLRWQHPTRGLLGPIEFIGVVEETGLIRELGWWNLSEACRQISEWRAASKANRDLIIS
                     VNLSAKQFLQPNLVADISSLLREINLPPEALKLEITESTVMKDPSAAVEMLQQIKSLG
                     VRLAIDDFGTGYSSLSYLHRFPLDTLKIDRSFISSMDDDGDGMEIARTILPMAKNLRL
                     DVVAEGV"
BASE COUNT          271 a          331 c          324 g          278 t
ORIGIN      
        1 gacgccttcc gctaccacat ctagccgcag gtttttggcc atcggcaaaa tggtgcgcgc
       61 tatttccatg ccgtcgccat cgtcatccat gctgctgatg aaagagcggt cgattttcaa
      121 cgtgtccagg ggaaaacgat gcaggtaact gagggaagaa tatccggttc cgaaatcgtc
      181 tatggccaga cgcactccca gcgatttaat ttgctgcaac atctcgactg cggcggaggg
      241 atctttcatc accgtgcttt cggtgatttc cagctttaaa gcttccggcg gaagatttat
      301 ttcacgcaat aagcttgaaa tgtccgcgac aagattgggc tgcaagaatt gtttcgcgga
      361 gagattcaca ctaatgatca gatcgcgatt ggccttggaa gccgcgcgcc attcgctgat
      421 ttgccggcac gcttcgctca aattccacca gcctaattca cgtatgaggc cggtctcttc
      481 cacgacaccg atgaattcaa tgggccccag aaggccgcgc gtaggatgct gccagcgcag
      541 caaagcttca aatccggcaa tttctccgga agcgagattc acgataggtt gataaaaatt
      601 gcggaattcg ccgcgttcca aggcgtgacg caagtcagtc tcgagctgca aacgggccat
      661 gacactggcg cgcattttcg cgtcgaatac ctcatagcgc ccttttccaa gagacttagc
      721 gtgatacatg gcggtgtccg catcgcgcaa catctcgtct ggtccgtcgt acaccggatt
      781 gctctgcgca attccgatgc tgacagaggt gtatacctct ttacctgcga gaagaaatgg
      841 gggcttgagc gccttcatca ggcggtcggc cgcgatcttc gcgtcgttgg gatctttgag
      901 gtcgtcgagc aacacggtga attcatcgcc gcccagtcgc gccacggtgt aagtgtccct
      961 caagcgcgcg agggtgtcgg aagcacgcaa acatttctcc agccggtccg ccacgccaat
     1021 cagcaactga tcgccgacca tgtgacccat gctgtcgttg atcattttga agccgtcaag
     1081 atccatgaac acgaccgcga aggaatggtc tttgcgccgc ttggaatatt tgatgagccg
     1141 gttcagccgg tcaatgaata aaagccggtt tggcaagccg gtcaggggat cggacacttt
     1201 tgcc
//
LOCUS       DATCBB010000139         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_134072, whole genome shotgun sequence.
ACCESSION   DATCBB010000139 DATCBB010000000
VERSION     DATCBB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134072"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VII37_01625"
     CDS             <1..1254
                     /locus_tag="VII37_01625"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY5068445.1"
                     /translation="DLTPIGGANSEQGGLVTFLFGPRLNLRKFDHFVPFAEALIGGAH
                     AGSAVTGDRSQYTFAAALGGGVDIVLWKNLAWRFAQIDYLMTNFAGPALHVNGQNNLR
                     AATGVVFRFGIPNPPPPPNHPPVAACSANPTSVYAGSGDSVAVHITASDPDNDNLTYS
                     YTATGGTVEGTGPDGRFNTNGLNEGNYTVNVKVDDGRGGTATCSADVKVEPRPNRPPT
                     ISCAADRTPIQAGERTGITANASDPDNDPLTYSYSASAGQVTGNGPKAEFDSTGLSAG
                     SYTVKCSVSDGRGGNADASTTVDVQQPPPPPQATKVGDCNYDKPGASRVDNVCKRVLD
                     DVSLRLKNDPNAKLVITGYADSREPRAAKLADTRADLARKFLGEKGIDASRITTRTGT
                     ASKEKGAEKENRRVEFIFVPEGATY"
     gene            1577..2041
                     /locus_tag="VII37_01630"
     CDS             1577..2041
                     /locus_tag="VII37_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5068446.1"
                     /translation="MKPWLGNVADPSPNLQHHANRILVVEDEPTVARLIADALEDEGF
                     QVDVQLDGREARQQADRESYDLVICDMKMPGLDGQHFYQALAQAGNPLSKRFLFVTGD
                     IVAQHTQQFLEHHQLPHVAKPFRMEELKDCVRGLLSRDFSGEPKTQEARNNG"
BASE COUNT          551 a          627 c          608 g          443 t
ORIGIN      
        1 gatttaactc cgattggcgg cgcaaattct gaacagggcg gattggtaac cttccttttt
       61 ggcccacgct tgaatctccg caagtttgat cactttgtgc cattcgccga agcgttgatt
      121 ggtggcgccc acgccggatc ggcggtcacc ggtgaccgat cgcaatatac ctttgccgcg
      181 gcgctcggcg gcggtgtgga tatcgtgtta tggaaaaacc tggcttggag attcgcacag
      241 attgattacc tgatgaccaa tttcgccggc ccggcactgc acgtgaacgg tcaaaataat
      301 ttgcgcgctg ctacaggcgt ggtgttccgc tttggaattc ccaatccgcc tccgccacca
      361 aatcatccac cagtggcagc ttgctcggca aatccaacgt cagtgtacgc cggctcaggt
      421 gacagcgtag cagtgcacat taccgccagc gatcccgata acgacaacct cacctatagc
      481 tatacggcaa ctggtggaac cgtagaagga acgggcccag acggtcgctt caataccaac
      541 ggactcaacg aaggaaatta cacagtgaac gttaaagtcg acgacggccg gggcggcacc
      601 gctacctgct cggccgatgt gaaggtcgaa ccgcgaccca accgcccacc gacgatctct
      661 tgcgccgctg accgcacccc gattcaagcg ggtgagcgca caggcatcac tgcaaatgcc
      721 agcgatcctg ataacgatcc gctaacctac agctatagcg cctcggccgg gcaggtaaca
      781 ggtaatggcc cgaaggccga gtttgattcc acgggcttgt cggccgggag ttacaccgtg
      841 aagtgctcgg ttagcgacgg ccgcggaggt aatgcggatg catcgaccac cgtcgatgtg
      901 cagcaaccgc cgccaccacc gcaagccacc aaggtcggcg attgcaacta cgacaagccg
      961 ggcgcctcgc gagtagacaa tgtctgcaag cgcgtgctcg acgacgtatc attgcgcctc
     1021 aaaaacgacc ccaacgccaa gctggtgatt accggttacg cggattcgcg tgaaccacgt
     1081 gcagccaaac tggccgacac gcgagccgat ctcgccagaa aattcctggg cgaaaagggc
     1141 atagacgcgt cgcgcatcac cacccgcacg ggtaccgctt cgaaggaaaa aggcgcggag
     1201 aaagaaaacc gccgcgtcga attcatcttc gtgccggaag gtgcaaccta ctaaactcgt
     1261 aatttgccaa tgatgccttc gagcgtcgtt ggttcaaacc cacacgggcc ttcctcgaaa
     1321 cgggaaggcc cgtttttttg gccgtccatt gccatttaaa atgacataaa tgcgaaattg
     1381 agaaaaatca gcctgccagc agttcccgta atgggtagca caaatcgttt cgggaaacgc
     1441 cgcaaagcga acaattgcca gcgccgcacc gcagaatcga taaattgggc ctggtgaatt
     1501 tccccgaaat gatatgcagg aaatgataat attataggta ttgtattgat tagtgaatag
     1561 ttggccgttt cctgaaatga aaccctggct gggtaatgtc gcagacccgt cccctaacct
     1621 gcaacaccat gccaatcgta ttttggttgt ggaagacgag cccacggtag cacggctgat
     1681 cgcagatgct ctggaggatg aaggctttca agtagacgtc caactcgacg gacgcgaggc
     1741 ccggcaacag gctgatcgag agtcatatga cctggtcatt tgtgacatga aaatgccagg
     1801 gcttgatgga cagcatttct accaggcgct agctcaagca ggaaatcctc taagcaagcg
     1861 gtttctcttc gttaccggcg acatagtggc ccagcatacc cagcagttcc tggaacacca
     1921 ccaattgccg cacgtagcca agccttttcg catggaggaa ttgaaagatt gtgtgcgcgg
     1981 tttgttgagc cgcgattttt ccggggagcc caaaactcaa gaagctagaa acaatggatg
     2041 aggtgcaatt aacgtgagat ccgatgtacg acaaaccatg ctggtagtaa gcaacgacac
     2101 cttgctctgc gctgcggcgc gccgcgaact cgaatcccgc agactggatg tgcgcgtgtc
     2161 agctgtaagc agcgtggaaa ccgcgctgca aatcattgaa gaagccgcgc ccggtgtcgt
     2221 gctgcttgc
//
LOCUS       DATCBB010000140         3440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_393603, whole genome shotgun sequence.
ACCESSION   DATCBB010000140 DATCBB010000000
VERSION     DATCBB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393603"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VII37_01635"
     CDS             complement(<1..1194)
                     /locus_tag="VII37_01635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5068447.1"
                     /translation="MTVSDSRIGQILSHYKIARTLGSGGMGVVYEAEDTLLGRRVAVK
                     FLPEAMAQDNQLIERFQREARAASALNHPNICTIHAIEQYGGQHFIVMELLEGQTLGQ
                     LMTRQPFAMDKLLPLATQIADALESAHAKGIVHRDIKPANLFVTARGQVKVLDFGLAQ
                     IGTAEIFPPGAATDSKMDTMAGGGLTSLGSTVGTVLYMSPEQARGQLVDARTDLFSLG
                     TVLYQMASGKTPFQGDTAAVIFDAILNREPTSLTVENPAAPAQFGNILAKLLEKDRTL
                     RCQSATELKTDLNRLKRDLESGSKQAASSSDSKNGSAKSAPGTKSVAVLYFENLSGVR
                     EDEYLRDGITEDIITELSKIRGINTYSRPTVLTFRDKQVTPAQIGQQLKAAYVLTGSL
                     RRSGTR"
     gene            complement(1254..2051)
                     /gene="xth"
                     /locus_tag="VII37_01640"
     CDS             complement(1254..2051)
                     /gene="xth"
                     /locus_tag="VII37_01640"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY5068448.1"
                     /translation="MIFILTEWEYALKIASWNVNSIRARIEHVTAWLTANAPDVLLLQ
                     ELKGAEFPDAAFKQLGYESVAVTQKAYNGVATLSRLPIETINKALAGDEADSHARFLE
                     TTIADTRIVNIYLPNGNPPSSVNFAYKLAWMDRLLNEMIAWKNKNVPVIIGGDFNVIP
                     EDIDCHKPSSWMHDALFQPEPRARYRAMLQLGYTDAFRSLHPGETGQFTFWDYFRQAF
                     ANNRGIRIDHFLLSPQLAKKLVSCQIDKGPRGKEKPSDHAPIILELR"
     gene            2066..2299
                     /locus_tag="VII37_01645"
     CDS             2066..2299
                     /locus_tag="VII37_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068449.1"
                     /translation="MSEICVHCYSGYRADQRPSRFIIRDREYLVEELDDQWYSPDATY
                     FRVRADDGNYYVLRHNEAQHSWTLDGYRQARAQ"
     gene            complement(2455..>3440)
                     /locus_tag="VII37_01650"
     CDS             complement(2455..>3440)
                     /locus_tag="VII37_01650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5068450.1"
                     /translation="VASAVALLLQRGNSTMRGRSLEEGSLCGTCYDTGTTKCEKDQSL
                     LTLVPTPRMLAQRYRLEKRLGQGGMGKVYRATDVSLDRTVAVKMIRDELFANQSAMEK
                     FRQESRVTASLAHPNVVTVHDFGVDTNQHVFLVMELLEGITLREEFSQKTRVTPQRTL
                     ELFAGICAGMRAAHAQGLVHRDLKPENIFVSKPKVLEVVKITDFGIAKTLDEFANDAN
                     ATATGVLVGTMRYMSPEQLQGKSISARWDVWALGVIAYEALCGTAPFTGTDYATLRGA
                     IIGTAFPKVATLVPEAPDKWQDFFVRVFAPLEEQRPQSVEVFWQELKACLG"
BASE COUNT          714 a          995 c          928 g          803 t
ORIGIN      
        1 gcgggttccc gaacgccgta aacttccggt caagacgtaa gccgctttaa gttgttggcc
       61 gatctgtgcg ggagtcactt gcttgtcgcg aaaggtcagc accgtgggcc gcgaataggt
      121 attgattccg cgaatcttgg ataattccgt gatgatgtct tcggtaatcc catcgcgcag
      181 gtactcgtct tccctgacgc cgctcaagtt ttcaaaataa agcaccgcca ccgatttcgt
      241 gccgggagcc gacttcgccg aaccgttctt actatcgctc gagctggcag cctgtttact
      301 tcctgattcc agatctcgct tgaggcgatt caggtcggtc tttaactctg tcgctgactg
      361 gcaacgcagc gtgcgatctt tttccaggag cttcgctaaa atatttccaa attgcgcagg
      421 cgccgcagga ttctccacag ttaacgaagt cggctcgcga ttcagaatgg catcgaaaat
      481 gacggccgca gtgtcgccct gaaaaggagt tttcccgctg gccatttggt aaagcaccgt
      541 ccctagcgaa aacagatcgg tcctggcgtc caccaactgt ccgcgcgcct gttccggtga
      601 catgtacaga accgtgccca ccgtagaacc tagtgacgtc agccctccac cggccatggt
      661 gtccattttt gagtcggtcg ctgctcccgg cggaaagatc tccgcggttc caatctgcgc
      721 tagcccgaaa tcgaggacct tcacctgacc gcgcgcagtg acaaagaggt tggcgggctt
      781 aatatcccga tgcacaattc ctttggcgtg cgcggactcc agcgcatcgg caatctgggt
      841 ggccagcggc agcaatttat ccatggcaaa cggctggcgc gtcatcagct gccctagggt
      901 ctgcccttcc agcaactcca tgacaatgaa gtgctgccca ccgtactgtt caatggcgtg
      961 gatggtgcaa atattcggat gattcagcgc cgaggccgcg cgagcctcgc gctggaagcg
     1021 ttcaatgagc tgattgtcct gggccatcgc ttcgggcagg aatttcacgg ccacacgacg
     1081 accgagcaga gtgtcctcgg cttcataaac cacacccatc ccgccactgc ctagcgttcg
     1141 tgcgatttta tagtgcgaaa ggatttgccc aatgcgggaa tcggaaacag tcatggctgg
     1201 cgataatatt ctgctgctat ccggcggtca agcgggctgc tctcggctgt gcgtcaacgc
     1261 aattcgagga tgatgggggc gtgatcggag ggcttttctt tgccgcgcgg gcccttgtcg
     1321 atctggcagg agactaattt ttttgcgagt tgtggcgaca gcaggaaatg atcgatgcgg
     1381 atgccgcgat tattcgcgaa ggcctgccgg aaataatccc agaaggtgaa ctggccggtt
     1441 tcgccgggat gtagcgagcg gaaggcatct gtgtatccaa gttgtagcat ggcgcggtac
     1501 cgcgcgcgcg gctccggttg aaacagcgcg tcgtgcatcc atgacgaagg tttgtggcag
     1561 tcgatgtcct cgggaatcac gttgaagtcg ccgccgatga tcaccggaac atttttattt
     1621 ttccaggcga tcatttcgtt gagcaaacga tccatccagg ccagcttgta ggcgaaattg
     1681 acgctgctgg gaggattgcc gttgggaaga tagatattca cgatgcgcgt gtcggcgatg
     1741 gtggtttcca gaaagcgcgc gtgactgtcg gcctcatcgc cggccagcgc cttgttgatg
     1801 gtctcgatgg gcaggcgcga aagggtggcg acgccgttgt aggctttctg cgtaaccgcg
     1861 acgctttcgt agcccaactg cttgaacgcg gcgtccggaa attccgctcc tttcaattcc
     1921 tgcaacagca ggacgtccgg cgcgttggcc gtgagccatg ccgtaacatg ctcgatccgc
     1981 gcgcgtattg aattaacgtt ccaactggcg attttcaggg catactccca ttcagtcagg
     2041 atgaaaatga ttccggaggc ggcttatgag cgaaatttgc gtgcactgtt attcaggata
     2101 tcgcgctgac cagcgtccct ctcgctttat catccgcgat agagaatacc tcgtcgagga
     2161 actcgacgac caatggtact cgcccgacgc gacttatttc cgagtgcgcg ccgacgacgg
     2221 aaactactac gtgctgcgcc ataatgaagc ccagcattcc tggacgctcg acggctatcg
     2281 ccaagcaaga gcgcaatagc gccagcaaag tttattcccc agcccgtcgc catgagccct
     2341 gcgactgacg gcgcgtacaa gtacggacaa ctctgaagcg aaaagtaatt tttgtcggga
     2401 tcttggcaac gccagaaaaa ttctcccagt tcttgcttca aaatccgctg tctttcatcc
     2461 caagcaggct ttaagctcct gccaaaacac ttccacgctt tgcggtcttt gctcttccaa
     2521 cggcgcaaag acacgtacaa aaaagtcttg ccatttatca ggcgcctccg ggaccagcgt
     2581 agcgacctta ggaaaagccg tcccgatgat agccccgcgc agcgttgcgt aatccgttcc
     2641 cgtaaacggc gcggttccgc acaacgcttc gtaggcaatt acgcccaacg cccagacgtc
     2701 ccatcttgcg gatatcgact tcccttgtag ctgttccggg gacatataac gcatggtgcc
     2761 gaccaaaacg ccggtagccg tggcattcgc gtcgttcgcg aattcgtcca gtgtttttgc
     2821 aatgccaaag tcggtgatct ttaccacttc gagtaccttc ggtttcgata caaatatatt
     2881 ctccggtttc agatcgcgat gcaccaggcc ttgagcgtgc gccgctctca ttcccgcaca
     2941 aatgcctgca aacaattcca gcgttcgctg gggtgttact cgtgttttct ggctaaactc
     3001 ctcgcgcagc gtaatcccct ccaacaattc catgaccagg aatacatgtt ggttggtatc
     3061 cactccaaaa tcgtggacgg ttactacatt cggatgcgcc aggctggcag tcacccgcga
     3121 ttcttgccgg aacttttcca tggcgctctg gttcgcgaag agttcgtcgc ggatcatttt
     3181 tactgccacg gtgcggtcca gtgagacgtc cgtggcccgg taaactttcc ccattcctcc
     3241 ctggccaagc cgcttctcca ggcggtatcg ttgcgccagc atccgcgggg taggcaccag
     3301 cgtaagtaac gactgatctt tttcacattt tgtagttccc gtgtcgtagc acgttccgca
     3361 caacgaaccc tcttccagcg agcggccccg catggtgcta ttgccccgtt gcagcaatag
     3421 cgcgaccgca ctcgcaacat
//
LOCUS       DATCBB010000141         1022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_264118, whole genome shotgun sequence.
ACCESSION   DATCBB010000141 DATCBB010000000
VERSION     DATCBB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264118"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1022
                     /locus_tag="VII37_01655"
     CDS             <1..>1022
                     /locus_tag="VII37_01655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY5068451.1"
                     /translation="DLGSPGFSSLNVRNPEFFQKLNGILDSESLDALTIYASWHVLKL
                     AAPWLSDSFVQADFQFRHNLTGQAEIQPRWKRCVSATDDALGEALGERYVDLTFGADG
                     KQRMLKMVLALEQALGEDIDGLPWMSAETKKQAHVKLAAINNKIGYPDQWRDYSKLTI
                     ARSDLMGNYLRANEFESRRDVNKIEKPVDRSEWGMTPPTVNAYYDPSLNEIVFPAGIL
                     QPPFFDKQMDDAINFGGIGLVIGHELTHGFDDQGRKFDPQGNLRDWWTPGDGTEFEKR
                     ASCVANEYGSFVSVDDLKLNGKLTLGENTADNGGARIALMALHHEMAQDPTAKTAKQI
                     DDYTPD"
BASE COUNT          243 a          255 c          306 g          218 t
ORIGIN      
        1 agatctcggg tcgccgggct tcagttccct caacgtgcgc aatccggaat tctttcagaa
       61 gctaaatgga attttggact ccgagtctct tgatgccctg acaatttacg ccagctggca
      121 tgttttgaag ctggccgctc cctggctgtc agattcgttt gtgcaagcgg actttcagtt
      181 ccggcacaat ttgacaggcc aggcggaaat ccagccgcgc tggaagcgtt gcgtgagcgc
      241 cactgacgat gccctcggcg aagctttggg cgaacggtac gtcgacctga cgtttggcgc
      301 ggatggaaag caacgaatgc tgaagatggt gctcgcgcta gaacaggcgc tgggtgagga
      361 cattgatggc cttccgtgga tgtctgcgga gaccaagaag caggcgcatg taaagctcgc
      421 agcgattaac aataagatcg ggtatcccga tcaatggcgg gattacagca aactcacaat
      481 tgcgcgcagc gacttgatgg ggaattatct tcgggccaac gaattcgaat ccaggcgcga
      541 tgtgaataag atcgaaaaac ccgtggaccg aagtgaatgg ggcatgaccc cgccgacggt
      601 gaatgcctat tacgatccgt cattgaacga gattgttttt cctgcgggaa ttttgcagcc
      661 gccatttttc gacaagcaga tggacgatgc catcaatttc ggcggcatcg ggctggttat
      721 cggtcacgaa ctgacgcacg gctttgacga tcaaggccgc aagtttgatc cgcaaggcaa
      781 tctgcgtgac tggtggacgc ccggggacgg cacggagttt gaaaagcggg caagttgcgt
      841 cgcaaatgaa tatggcagct ttgtctcggt cgatgacctc aagctgaatg gcaagctgac
      901 gctgggggaa aacacggccg ataatggcgg cgcgcgcatt gctttgatgg cgctgcatca
      961 tgaaatggcg caggacccca ctgcaaagac cgcgaaacag attgatgact acactcctga
     1021 cc
//
LOCUS       DATCBB010000142         1016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_134126, whole genome shotgun sequence.
ACCESSION   DATCBB010000142 DATCBB010000000
VERSION     DATCBB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134126"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..>1016
                     /locus_tag="VII37_01660"
     CDS             155..>1016
                     /locus_tag="VII37_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5068452.1"
                     /translation="MSEPVAKLSFWGVRGSTPTVDPGTWRYGGNTPCLELVTPDGIQI
                     ILDCGTGLRMLGSRWVAPSAGEARETHILVTHYHWDHIQGIPFFAPLYDEKNHFHFYS
                     FRSKYLGRDSLKQVFETQMALPYFPVNMSAMNAKRKFQEVDGGDSFRIGKNKVTARWL
                     NHPQGCLGFRIETSAGTVVYATDNEPGDAKLEKSLRELATGADIFINDAQFTPEQLAT
                     TKKGWGHSSWKEGVKVAREVGATTLILFHHDPDSADRAVDTILRQAREEFDSVFAASE
                     GMVITLGAPGD"
BASE COUNT          230 a          238 c          331 g          217 t
ORIGIN      
        1 cacccgcgct acgtgaattg attgacggtc tgaccagcga ctgccacgat gacaacagct
       61 tctgagagca cctgaacatt ttacgtagtt cttgcattga ggttggttgc gggatattac
      121 ggtgtccgca aggggcgata gccggattat ctaaatgagt gagcccgtag ctaaattgag
      181 tttttggggt gtgcgcggat ccacgccaac ggtggacccg ggcacgtggc ggtatggcgg
      241 gaacacgcct tgccttgagc tggtgactcc ggacggaata caaatcattc tggattgcgg
      301 gacgggattg cggatgctgg gcagccgctg ggttgcgcca agcgcgggcg aggcgcggga
      361 gacacatatt ctggtgacgc attatcactg ggatcacatc caggggattc cgttttttgc
      421 gccgctgtat gacgaaaaaa atcattttca cttttacagt tttcgctcga aatatctggg
      481 gcgcgacagt ttgaagcaag tgttcgagac gcagatggcc ttgccgtatt ttccggtgaa
      541 tatgtcggcg atgaatgcca aacggaaatt tcaggaagtg gatggcggcg acagctttcg
      601 catcggcaag aataaggtca cggcgcggtg gttgaatcac ccgcagggct gcctgggctt
      661 tcgcatcgag acttcggcag gaacagtggt gtacgccacg gacaacgagc cgggagatgc
      721 caagctggaa aagagcctca gggaattagc taccggagcg gacattttta ttaatgacgc
      781 gcaattcacg ccggaacaat tggcgaccac gaagaagggc tggggacact cgtcgtggaa
      841 ggaaggcgtg aaggtggcgc gggaagtggg cgcgaccacg ctgatacttt tccaccatga
      901 tccggattca gcggaccgcg cggtggatac gattttgcgg caagcgcgcg aagaatttga
      961 ttcggttttc gcggccagcg agggcatggt gatcacgctc ggagcgccag gagacc
//
LOCUS       DATCBB010000143         1785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_101999, whole genome shotgun sequence.
ACCESSION   DATCBB010000143 DATCBB010000000
VERSION     DATCBB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101999"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VII37_01665"
     CDS             complement(<1..316)
                     /locus_tag="VII37_01665"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068453.1"
                     /translation="MPTQTLTTDVRMIKGVGPQRAELLAKRGIHTLEDLLTYLPFRYE
                     DRIHFSNIKDIQPNGVYTIRVQVMSGQAVRTMRGRDAVFHLLVRDATGQLPCKFFHGG
                     YLE"
     gene            645..>1785
                     /locus_tag="VII37_01670"
     CDS             645..>1785
                     /locus_tag="VII37_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068454.1"
                     /translation="MSTDRLTSANKLALLLWVIAGIAGALFAYKYYFRAFPEASVNFK
                     VSRAEALARAKEFLASEGQNAASYQSAIVFDVDDNAKTYLERETGLEQANRLMSSELN
                     IWYWDVRFFKPQQEEEFHVRVSPAGQVVGYEHHVEEARAGATLQRAAAQYAAENFLES
                     KLGIQSGWDLLPEEINSVKRPNRLDWSFTWEKHGFRAKDAPYRLNVVLQGDQIGNSEE
                     YLKVPEAWERSYKQLRSTNLLYNQIAIIPYILLMGSALWLGISLWRKGQADWMGAIKL
                     GGLVAVLFFFMQLNQWQSTRANYDTHASYGSFVALQILLNILLAIGTALTVTLALPGA
                     EPLYRASQPDRLRLSKAFTLRGLQSKEFFSAAVVGLSLAAAHIGFI"
BASE COUNT          406 a          441 c          515 g          423 t
ORIGIN      
        1 cctccagata gccgccgtga aaaaatttgc agggcagttg gccggtggcg tcgcgaacca
       61 gcagatgaaa aactgcatca cgcccgcgca tagtgcgcac cgcctggccg ctcatgacct
      121 gcacgcgaat ggtgtaaacg ccgttgggct gaatgtcttt gatatttgaa aaatggatgc
      181 ggtcttcgta acggaaaggc agataggtca gcaggtcttc gagggtatga attccgcgct
      241 tggccagcaa ctccgctcgt tgcggaccaa cgcccttgat catgcgtacg tcagtagtga
      301 gggtttgcgt gggcacgatg aaaagcgttc gatttgcgaa cgccgattga gattctgctc
      361 cagccgttcg cacagtaacg gcaaaataac tccatctttt ataccattat ggacatgcgg
      421 cactgtgata agcggctgtg caggcgaaac ttacaccgat tttgtgcgta ttctagagtg
      481 atggcgccgc actctcattt aaggatacgt cttagtttgt agctttccaa agtcccacgc
      541 gcaaccgcgg tgtgtggggt accctctttc atgcgaagct taaattgctg ctgctgaatt
      601 aaattgatgc atagatgtag attggagtgc tttgtggagg ttccttgtct actgaccgcc
      661 tgacatctgc gaacaaactt gcgttactgc tgtgggtgat cgcaggaatt gccggagcat
      721 tattcgcgta caagtattat tttcgcgcgt ttccggaagc gtcggtgaat tttaaggtgt
      781 cgcgtgcgga agctctagcg cgtgcgaaag agtttttggc gagtgaaggt cagaacgccg
      841 cgagttacca gtccgcaatc gttttcgatg tcgacgacaa cgcgaaaaca tatctggaac
      901 gtgagaccgg cctcgagcaa gctaaccggc tgatgtcttc tgaactgaat atctggtatt
      961 gggatgtgcg ttttttcaag ccgcaacagg aggaagagtt tcacgtacgg gtgagtcccg
     1021 cgggccaggt ggtgggctac gaacatcatg tcgaagaagc ccgagccgga gcaacgctgc
     1081 agcgcgccgc cgcccaatat gcggccgaga attttcttga aagcaaactg ggcatccagt
     1141 cgggctggga tcttttaccg gaagaaataa attcagtgaa aagaccgaac cgcctggatt
     1201 ggtcgttcac gtgggaaaaa cacggatttc gcgccaaaga cgcgccgtac cggctgaatg
     1261 tcgtgctgca aggcgaccag atcggaaaca gcgaagaata tttgaaagtg ccggaagcgt
     1321 gggagcgcag ctacaagcaa ttgcgctcca ccaatctgtt gtacaaccag atcgccatta
     1381 tcccgtacat cctgctgatg ggcagcgcgc tgtggctggg catttcgctg tggcgcaaag
     1441 ggcaagccga ctggatggga gctataaagc tgggcggcct tgtagccgtg ttgttttttt
     1501 tcatgcaact caatcagtgg caatccacgc gagcaaatta cgatactcac gcctcgtacg
     1561 gaagttttgt cgcgctgcaa atccttttaa acatattgct ggctattggc acggcgctca
     1621 ctgtcacttt ggcgctgccg ggtgccgagc cgctgtatcg cgcgtcgcaa ccagaccgct
     1681 tgcggctcag caaggcattt acgttgcgcg gactgcaatc caaggaattt ttctctgccg
     1741 cggtggtggg cttgtcgctg gcggcagcgc acatcgggtt tatcg
//
LOCUS       DATCBB010000144         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_102023, whole genome shotgun sequence.
ACCESSION   DATCBB010000144 DATCBB010000000
VERSION     DATCBB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102023"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VII37_01675"
     CDS             <1..773
                     /locus_tag="VII37_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068455.1"
                     /translation="AFFGSVLIVRRYFDGWTFPSWVHFLANPAWVWPFLILFSAQVRE
                     IMVQYVGDVAIYVRPNKLDRFDAVRTEIKDAARSVASALFTSYELQTDPGAAPKFQYD
                     KIALVGHSLGSVIAYDTLNRLMLDDWLCDGALKVAERTATMVTFGSPLNKTAFLFTIQ
                     GKDTLHIRERLAATVQPLIMSYPKFRKFKWINVYSRNDIVSELLKFYDLPGYQNPPVP
                     AAAVQNVKDRDAAVPLMAHVAYWKNKTVWTQLLEQIAP"
     gene            complement(808..>2412)
                     /locus_tag="VII37_01680"
     CDS             complement(808..>2412)
                     /locus_tag="VII37_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HEY5068456.1"
                     /translation="AAQTSLGHYWQKITDRTFSGVYHANGFDLAMMIPYFIVLFILAL
                     YGLHRYWLVYDYYAYRKNVPAQPPAVTEWPRVTIQLPIYNERYVIERLVEAISRFDYP
                     TELLDVQVLDDSTDETQQVARNCVERFAAQGMAITYIHRSNRAGYKAGALENGLKSSK
                     GEFVAIFDADFVPASDFLQRTIPYFKNPNGGAGIGMLQTRWTYLNTDYSLLTQVETIL
                     LDGHFVVEHGARSRRGTFFNFNGTAGVWRRKAIDQAGGWQHDTLTEDTDLSYRAQLVG
                     WKFLYLPHIECASELPVDMNGFKAQQARWAKGLMQTAKKILPKVFKANVPWHVKSEAF
                     FHLTANISYPLMVLLSTMLLPAMIVRFYQGWIQMLVIDLPLFLASTCSISSFYLVAQK
                     ELHPKTWYRTFLYMPFVMATGIGISVRNAQAVIEAIAGRKSEFARTPKYNIAGKQGSY
                     AKKSYKNKAGWMPYAEIFLGVYFALTVVYAVANENYATVPFLLLFVWGYLYTGFMSLG
                     QTYLANLRFEVETPEMRPAASGAPGF"
BASE COUNT          574 a          597 c          687 g          554 t
ORIGIN      
        1 tggcgttttt tggttctgtt ttgatagtgc gccgatattt tgatggctgg actttcccaa
       61 gttgggtgca ttttcttgcg aaccctgcgt gggtttggcc gtttctcata ttgttcagcg
      121 cccaggtgcg cgaaataatg gtgcaatacg ttggcgacgt ggcgatttat gtgcggccca
      181 acaagctgga tcgctttgac gcggtgcgaa cagaaatcaa ggacgcggcg cgctccgtgg
      241 cgtcagcgct gttcacttct tatgagctgc aaaccgatcc gggcgctgcg ccgaaattcc
      301 agtacgacaa aatcgcgttg gtggggcatt cgcttgggtc ggtgattgcg tatgacaccc
      361 tgaaccggtt gatgctggat gactggctgt gcgatggcgc tttaaaggtg gcggaacgca
      421 ccgcgacgat ggtgacgttc ggctctccgc tgaacaagac ggcttttctt tttacgattc
      481 aggggaaaga tactctgcac atccgcgagc gattggctgc cacggtgcag ccgttaatta
      541 tgagttatcc aaaatttcgc aaatttaaat ggatcaatgt gtattcgcgc aacgatatcg
      601 tttccgagct cctgaaattt tatgacttac ctggatacca gaatccgcct gttccggctg
      661 cggcggtcca aaatgtgaaa gatcgcgacg cggcggttcc tttgatggcg catgtggcct
      721 attggaaaaa caaaacggtg tggacgcaac tgttggaaca aatcgcgccg tagcggcccc
      781 tagaagttcg ctaagttgcc gaaaggttta gaagccggga gccccggagg ccgcggggcg
      841 catttccgga gtttcgacct cgaagcgaag attggccagg taggtttggc ccagcgacat
      901 gaacccggtg tacaagtagc cccatacaaa aagaagcagg aacggcaccg tagcgtaatt
      961 ttcattggcc acggcgtaaa caacagtgag cgcgaaatat acgcccaaga aaatttccgc
     1021 gtacggcatc cacccagctt tatttttata cgacttcttg gcgtaggaac cttgcttgcc
     1081 ggcaatgttg tatttaggcg tgcgcgcaaa ttcgcttttc cttcctgcaa tggcttcaat
     1141 caccgcttgc gcgttgcgca cggaaatacc aattccagta gccataacga aaggcatata
     1201 aagaaaggtt cgataccagg ttttggggtg cagctctttt tgcgccacca ggtaaaagct
     1261 cgaaatcgag catgtggaag ccagaaatag cggcaggtcg ataaccagca tctggatcca
     1321 gccctgatag aaacgcacaa tcatggcggg cagcagcatg gtggagagca acaccatcaa
     1381 cgggtagctg atgttagccg tgagatgaaa aaacgcttcg gacttgacgt gccatggcac
     1441 gttggcttta aaaactttag gcaaaatctt cttggctgtt tgcatcaagc ccttggccca
     1501 gcgcgcttgc tgagctttga agccgttcat atcgacggga agctcggagg cgcactcgat
     1561 gtgcggcaaa tataaaaatt tccagccgac aagttgcgcg cgataggaaa ggtcagtgtc
     1621 ttccgtgagt gtgtcgtgct gccagccacc agcctgatcg atggcttttc ggcgccacac
     1681 tccagcggtg ccattaaaat taaaaaacgt tccgcggcgc gaacgtgcgc cgtgctccac
     1741 gacaaagtgg ccatcgagca aaatggtttc cacctgcgtg agcagcgaat agtcggtatt
     1801 gagatatgtc cagcgcgtct gcagcatgcc gataccggcg ccgccgttgg ggttcttgaa
     1861 atagggaatg gttcgttgca aaaagtccga cgcggggacg aaatcggcgt caaagatggc
     1921 cacaaactcg cccttggaac tcttaaggcc gttttccagc gctccggctt tgtagcccgc
     1981 gcgattgctg cgatgaatat aagtgatggc catgccctga gcagcgaaac gctcgacgca
     2041 gttacgcgcg acttgctgcg tttcatccgt ggaatcgtct aaaacttgca catcgagaag
     2101 ttcggtgggg taatcgaacc gcgagatggc ctcgacgagg cgctcgataa catagcgctc
     2161 gttgtaaatg ggcaactgaa tggtgacgcg cggccactcg gtcaccgccg gcggctgtgc
     2221 aggaacattt ttacggtatg cgtagtagtc gtaaacaagc caatagcggt gcagcccgta
     2281 cagagccaga ataaacagca cgatgaagta cggaatcatc atcgccaaat cgaagccatt
     2341 ggcatggtaa acgcccgaaa aggtgcgatc ggtgattttt tgccagtagt ggccgagaga
     2401 tgtttgcgcg gc
//
LOCUS       DATCBB010000145         1010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_231348, whole genome shotgun sequence.
ACCESSION   DATCBB010000145 DATCBB010000000
VERSION     DATCBB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231348"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VII37_01685"
     CDS             complement(<1..323)
                     /locus_tag="VII37_01685"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5068457.1"
                     /translation="MFKLHGILPPIATPFDHRGELYKVKVQHNVEKWNRTGLTGYVVC
                     GSTGESVYLTTEEKLQMWDWVAEYAASDKVLVCGTGMESVRETVALTNAAAERGYKAA
                     MVRTP"
     gene            complement(373..666)
                     /locus_tag="VII37_01690"
     CDS             complement(373..666)
                     /locus_tag="VII37_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068458.1"
                     /translation="MMLLLVVLFLLAACPIFAQTPGASGMAPGEVKFFGAMLGLGLAG
                     GLCGIGQGKAVAGAAEAIARNPAAAAGIRFALILGLVFIETLTIYALAIVLKG"
     gene            complement(781..>1010)
                     /locus_tag="VII37_01695"
     CDS             complement(781..>1010)
                     /locus_tag="VII37_01695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5068459.1"
                     /translation="VIEIIGHFARMLSLTVRLYGNMFAGEQITNVFISLTYLVVPVIF
                     MGLHVFVSFLQAYVFTLLTMIYVSIGTAHEH"
BASE COUNT          252 a          295 c          267 g          196 t
ORIGIN      
        1 tgcggcgtgc gcaccatcgc cgccttatac ccacgctccg ccgcagcatt cgtgagagcg
       61 acggtctcgc gcacgctttc catgcccgtc ccgcacacga gaactttgtc ggaagcggcg
      121 tattccgcca cccaatccca catttgcaac ttctcttcgg tggttaagta aacgctctcg
      181 cccgtagacc cgcagaccac gtagccggtg agcccggtgc gattccactt ctcgacattg
      241 tgctggactt taaccttgta aagctcacca cgatggtcga aaggagtcgc gataggagga
      301 aggatgccgt gcagcttaaa cataggagag ccctgcaggg cgggtggtga gacccgcccc
      361 aaggaatacg ggctaacctt tcaaaacgat cgcgagtgcg tagatggtca gcgtttcaat
      421 aaacaccaga ccgagaatca atgcaaatcg aattcccgcg gctgccgcag ggttacgtgc
      481 aatcgcttcc gcggcgccgg caaccgcctt gccctggccg attccgcaaa gtcctccggc
      541 aagtcccagg cccagcatgg cgccgaaaaa cttcacttcc ccgggagcca tgcccgacgc
      601 gcctggagtc tgcgcaaaaa ttgggcaggc tgccagcagg aataaaacca ccagcagcaa
      661 catcattttg tttcgcattt ccgtgtacct tccttttcgt gtattaaggt tgcgccgagg
      721 aggtggttct atccgaaccg gggggaacgg tagctcacgc tcgagcgcga taatgccaga
      781 ttaatgttcg tgcgcagttc cgatgctcac atagatcatc gtgagcagag taaaaacgta
      841 agcctgcaag aaggaaacaa acacatgcaa gcccataaaa atcaccggga ccactaagta
      901 agtcagactg ataaaaacgt tggtaatttg ctcgcccgcg aacatgttgc cgtaaagacg
      961 aaccgtcagc gagagcatgc gggcaaagtg accgatgatc tcgatgacaa
//
LOCUS       DATCBB010000146         1530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_166854, whole genome shotgun sequence.
ACCESSION   DATCBB010000146 DATCBB010000000
VERSION     DATCBB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166854"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VII37_01700"
     CDS             complement(<1..865)
                     /locus_tag="VII37_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY5068460.1"
                     /translation="MTFGKWFLRFAVLAILVLSVHCPSYAQQKRKVIIDQDCAGPGGT
                     DMQAILALVNSPETEVLGITVLTGDAWRDEEVQHTLRLLEIIGRTDIPVVPGATFPLV
                     NSKEYIAKWEKLYGKVIYQGAWNYAKHNPVHGPYDIPPMPEGAPSTKASTEDAARFMV
                     RLVNKYPHEVTIYAAGPMTDLALAIALDAHFAELTKELIVMGGSINPRTDDPEFSLTP
                     RREFNFWMDPEASQAVLHAHWPRIVLTTVDISVQTRMEKGLIAEVAKSQTPAAQYVVK
                     DAEANYLWDELA"
     gene            complement(911..>1530)
                     /locus_tag="VII37_01705"
     CDS             complement(911..>1530)
                     /locus_tag="VII37_01705"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEY5068461.1"
                     /translation="ELDDIFAGNMKMPMLDEKLAVLHQIGAVLTEKYDGRFHKFVQAC
                     SPRLYDGGNGVIERMVKEFPRFNDVSEFEGHEIKFYKLAQLGVWMLYSTLHKSGKFRL
                     DDAEKMTAFADYIVPVALRLLGITWYSPELENVINAHQLIARDSRREIEIRAHCLYAT
                     ALLAEEVNKHRGPKEQVIIPQIDARLWTHYHTTSWPHHLTQTIMY"
BASE COUNT          346 a          468 c          349 g          367 t
ORIGIN      
        1 ccgctaactc gtcccacaga taatttgctt ccgcatcctt caccacatac tgcgctgcgg
       61 gcgtctgact tttcgccacc tccgcgatca agcccttttc catccgcgtt tgaacggaaa
      121 tatctaccgt cgtaagcacg atgcgcggcc agtgtgcatg aagaaccgct tgcgacgcct
      181 ctggatccat ccagaaatta aattcccgtc gcggcgtcag gctaaactcc ggatcgtccg
      241 tgcgcggatt aatactgccg cccatcacga tcaattcttt cgtcaattcc gcaaaatgcg
      301 catcaagcgc gatggccagt gccaggtccg tcattggtcc cgccgcgtaa atggtcactt
      361 catgcggata tttgttcacc aggcgcacca tgaaccgcgc tgcatcttcc gtagacgctt
      421 ttgtactggg cgcgccctca ggcatgggag gaatgtcata aggtccatgc actggattgt
      481 gtttcgcgta attccatgca ccctggtaga tcaccttgcc ataaagtttt tcccacttgg
      541 cgatgtactc tttgctgttc accagcggaa aagtcgctcc aggcaccact ggaatatccg
      601 tgcgcccaat tatttccagc agccgcaaag tgtgctgcac ttcctcatcg cgccaggcgt
      661 cgccagtgag caccgtgatt cccagcacct ccgtctcggg agaattcacc agagccagaa
      721 tcgcctgcat atccgtgcca cccggcccgg cacagtcttg atcgatgatg actttgcgtt
      781 tttgttgcgc gtaagaaggg cagtgcacac tcaacaccag gatcgcaagc acggcaaagc
      841 gtaagaacca tttgccaaat gtcatgaaat atttcctttc aagcaggcat agcacatttt
      901 cagtacagcc ttaatacatg atcgtctgcg tcaaatggtg aggccacgac gtcgtgtggt
      961 agtgggtcca aagccgcgca tcgatttgcg gaatgatcac ctgctccttc ggaccacgat
     1021 gcttgttgac ctcttccgcc agcagtgccg tggcgtagag acagtgcgcc cgaatttcaa
     1081 tttcacgccg cgaatcccgc gctatcaatt gatgggcgtt aataacgttt tccagttccg
     1141 gcgaatacca cgtaatgccc agcaaccgca acgctaccgg aacgatataa tccgcgaacg
     1201 ctgtcatctt ttccgcatca tccaaacgga atttccccga cttgtgcagc gtcgagtaca
     1261 acatccacac ccccagctgc gccaatttgt aaaattttat ttcgtggcct tcaaattcgc
     1321 tcacatcatt aaaacgcgga aactccttta ccattcgttc gatgaccccg ttccccccgt
     1381 catatagccg cggcgagcac gcctgcacga acttgtgaaa gcgcccatca tatttctcgg
     1441 tcaacaccgc gccgatttga tgcagcaccg caagcttttc atccagcatg ggcatcttca
     1501 tgttgccggc gaaaatgtca tcgagctcag
//
LOCUS       DATCBB010000147         1224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_426463, whole genome shotgun sequence.
ACCESSION   DATCBB010000147 DATCBB010000000
VERSION     DATCBB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426463"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VII37_01710"
     CDS             <1..185
                     /locus_tag="VII37_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HEY5068462.1"
                     /translation="EDKKVLSGSHAAKAKVTGKIVQQGRSKKVTVLKFKKTNQYKISR
                     GHRQNYTAVQVSDISL"
     gene            240..530
                     /gene="rpmA"
                     /locus_tag="VII37_01715"
     CDS             240..530
                     /gene="rpmA"
                     /locus_tag="VII37_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5068463.1"
                     /translation="MAHKKGGGSSTNGRDSHGQRLGVKRFGGEFVNAGTILIRQRGTK
                     HQAGVNVGQGKDDTLFALVTGIVKFQDKGRNGRFISVQPADEAKAPAKSVAN"
     gene            650..>1224
                     /gene="obgE"
                     /locus_tag="VII37_01720"
     CDS             650..>1224
                     /gene="obgE"
                     /locus_tag="VII37_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY5068464.1"
                     /translation="MFADEAKIFVKGGHGGNGCVAFRREKYVPRGGPSGGDGGHGASI
                     CLEANINDNTLLRYRYNREFKADRGRHGEGSNCSGQSGEDMVLLVPVGTVVFDAQTNE
                     TIADLATPGQRVLVAHGGRGGRGNQHFAKPWHQAPREHEEGQDGEERHLRLELKLLAD
                     VGLVGFPNAGKSTLISVISAARPKIANYPFTT"
BASE COUNT          291 a          328 c          355 g          250 t
ORIGIN      
        1 cggaagataa gaaggtgctt tccggatcgc acgccgccaa agcgaaggtc accgggaaga
       61 tcgtgcaaca gggacgttcc aagaaagtca ctgtgctgaa attcaagaag accaaccagt
      121 acaagatttc tcgcggccat cgccagaatt acacggcggt acaggtcagc gatatctcgc
      181 tgtaaaaatt aaaatcactc actgggattt aatgcaggtt tcccgaaaag gatttcatca
      241 tggcacataa gaaaggcggc ggctcctcta ccaacggccg cgattcacac ggtcagaggc
      301 tgggcgtgaa gcgctttggc ggagaatttg tcaacgctgg aaccatcctg attcgccagc
      361 gcggcaccaa gcatcaggct ggcgtcaatg ttgggcaagg caaggacgac actcttttcg
      421 cgctggtgac tggcatcgtc aagttccagg acaagggccg caacgggcgc tttatcagcg
      481 tgcaacctgc tgacgaagca aaagctcccg cgaaatctgt ggcgaactga acggtatcgg
      541 cactatgaat tcacgcggac ttcggtctgc gatttttttg cggtcccggt catccgggac
      601 cgttttgttt ctgcaacaat tcctccgtta aaagctaaaa ggacgtttcg tgttcgctga
      661 tgaagcaaaa atattcgtaa agggaggcca cggcggcaac ggctgcgtgg cgtttcgccg
      721 cgaaaagtat gttccgcgtg gcggcccatc cggcggcgac ggcggccacg gcgcgagcat
      781 ttgtctggaa gcgaatatta atgacaacac gctgctgcgg tatcgctata accgtgagtt
      841 taaagccgac cgtggccgtc acggcgaagg ttccaattgc agcggccaat ccggcgaaga
      901 catggtgctg ctcgttccgg taggcacggt ggtcttcgat gcgcaaacga atgaaaccat
      961 tgcggacctg gcaacgcctg gccagcgggt tctcgtcgcg catggtggcc gcggtgggcg
     1021 tggcaatcag catttcgcca aaccctggca ccaggctcct cgcgagcatg aggaaggtca
     1081 ggacggcgaa gagcgtcacc tgcgtttgga attgaaatta ctcgccgacg ttgggttagt
     1141 gggattcccg aatgcaggca aatccacatt gatctccgtg atttccgcgg cgcgccccaa
     1201 aatcgcgaat tatcccttca ccac
//
LOCUS       DATCBB010000148         1077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_134386, whole genome shotgun sequence.
ACCESSION   DATCBB010000148 DATCBB010000000
VERSION     DATCBB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134386"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..536)
                     /locus_tag="VII37_01725"
     CDS             complement(252..536)
                     /locus_tag="VII37_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068465.1"
                     /translation="MGLLVRKYPRKEPWRRRCTGQKLISRRRMGYEIAPCVLLMTFLS
                     PLASAQNAGADLSELSLDTLSNIEITSVARKAEKLSKAAAAIFVITQEER"
     gene            complement(836..>1077)
                     /locus_tag="VII37_01730"
     CDS             complement(836..>1077)
                     /locus_tag="VII37_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068466.1"
                     /translation="PSIIPITFIRILAVVGGVFLLMGQNWARWLLVAWLAFHVVVSAF
                     HSLPEMAAHAVLLIVAAYFLFTPPDTNYFRPSRSG"
BASE COUNT          237 a          317 c          296 g          227 t
ORIGIN      
        1 tgatcttccc gcagtgaatg gcgcgtgatc agcgctgcgt gagccagcca gctttttcgg
       61 ctcggttcct aacgcggtca aaatcgcatc cagcaattcg gcctgccgca ccggcttggt
      121 gagatatgcg gccacgccca attaacggca acggcgcgcg tcgccgcgct ggcccgcgga
      181 actgagcatc atgatcgtgg cggtttccca ttcgtacgtg agttccaggt ctttctggtg
      241 ggcgcgcgag ctcagcgctc ttcctgggtg atcacgaaga tggctgcggc ggctttcgag
      301 agtttctcgg ccttccttgc tacggaagta atctcgatat tggaaagcgt gtcgagactg
      361 agttcgctca aatcggcgcc ggcgttttgc gcggaagcca gtggagataa gaaagtcatg
      421 agcaatacac agggcgcgat ctcataaccc atccgccgcc gagaaattaa tttctggccc
      481 gtgcatcttc gccgccatgg ctctttgcgt ggatatttcc tgaccaaaag tcccattcga
      541 acgcctccgc ccgcttgaag gccggcggaa accgctttgg cctgcctgga aacgctgtta
      601 cgcgtcgacc caactgcatc gcgcgaaagc gtaagggttc aggcgtaccg atccaccgat
      661 tcaagttgtt tcgtgaatgc tcccagcgga ggggtaaaac tactggggca gtaatctcgg
      721 ttactttgac acatgctcag cgcacggagc tgtgcggaac atccgatgtt tccataaatg
      781 caaatccatt tcgtggcgcc gcttgtgacg ctctttcgcc cgcaaatcca gcctctcaac
      841 ctgaccgaga cggtcgaaaa tagttagtgt ccggcggcgt gaacaggaaa taggctgcaa
      901 caatcaacag caccgcatgc gcggccattt ccggcagcga gtgaaatgcg ctcaccacca
      961 cgtgaaatgc cagccacgcg accagcaacc agcgggccca gttctggccc atcagcagga
     1021 aaactcctcc cacaaccgca agtatccgta tgaatgtaat cggaataatc gacggat
//
LOCUS       DATCBB010000149         1206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_199091, whole genome shotgun sequence.
ACCESSION   DATCBB010000149 DATCBB010000000
VERSION     DATCBB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199091"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1206)
                     /locus_tag="VII37_01735"
     CDS             complement(<1..>1206)
                     /locus_tag="VII37_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068467.1"
                     /translation="RRMFADKKQNEFALQMDLAGEKNPQSQAVLEFWAALYNELNRES
                     KYFEIMTKLFEVYVRAANIPKAAETLEKLVDIDPYDQRNQERLEMLRNRVDDSLFKRI
                     SARLARSTSLSGGSPSTLHHSDTGEIVSASQGSSEAGREKQTLEDLIVQTEIFLQYSL
                     HNKALDRLQKIGVMFPGEEKRNARLSNLYQLANWWPPGAHRQQAGASPAGTTATPAAP
                     PAPAPMVMAPVPQAEPAGASAAGKSGVYTAETLRDLSKISEVNQKIFRQQTPRAMLNT
                     AVNEVGTYLRATRSLAVVGGAGRPPELTAEFCATDVKSAQLAQVVLLLALMEKAEPDE
                     LGGLMVESENGSVLAELGLESALGVIITDKETQTPAGMLIVGHARRHKWKPNEAYFLQ
                     AIGDQMLMSV"
BASE COUNT          226 a          404 c          289 g          287 t
ORIGIN      
        1 aacactcatt aacatctgat cgccgatggc ttgcaaaaag tacgcttcgt ttggcttcca
       61 cttgtgtcgc cgcgcgtggc ccacgatcag cattcccgcg ggcgtctgcg tttctttatc
      121 tgtaatgatc acacccagcg cgctctccag tcccaactcc gccagtaccg atccattctc
      181 agactccacc atcaagccgc caagctcgtc cggctcggct ttttccatca gcgccaataa
      241 aagcaccacc tgcgcgagct gcgccgactt cacgtctgtc gcgcaaaatt ccgccgtcaa
      301 ctccggtggg cggcccgcgc ctccaacgac cgccagcgat cgtgtcgcgc gcaggtacgt
      361 cccaacttca tttaccgccg tgttcaacat cgctcgcggc gtttgttgcc gaaaaatctt
      421 ttgatttacc tccgatattt tagacagatc ccgaagcgtc tccgccgtgt acaccccgct
      481 cttgcccgct gcactcgcac ccgcaggttc cgcttgaggc acaggcgcca tcaccatcgg
      541 cgcaggcgcc ggtggcgccg ctggtgttgc agtagttcct gcaggcgaag ccccagcctg
      601 ttgtcggtgt gcgccgggag gccaccaatt cgccagttgg tacaaattcg aaagccgcgc
      661 attgcgtttt tcctcgccag gaaacatcac cccgattttt tgcagccggt ccagcgcttt
      721 gttatgcagc gaatattgca aaaatatttc cgtctgcaca atcaggtctt ccagcgtttg
      781 cttctcacgc cccgcctcac tactgccttg cgatgcgcta acgatctcgc cggtatccga
      841 atggtgcagc gtcgaaggcg aacccccgct caggctcgtt gatcgcgcca gccgcgcgga
      901 aatccgcttg aacaacgaat cgtccacgcg gttacgcagc atctccagcc gctcctggtt
      961 gcgctgatca taggggtcaa tatccacaag tttttcgagc gtttccgcgg ccttcggtat
     1021 atttgcggcg cgcacatata cttcgaacaa cttggtcatg atttcgaagt atttcgattc
     1081 ccggttcagc tcgttgtaaa gcgccgccca gaattccagc accgcctgcg attgcggatt
     1141 cttctcccct gccagatcca tttgcagggc gaattcattc tgcttcttgt ccgcaaacat
     1201 ccgccg
//
LOCUS       DATCBB010000150         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_393971, whole genome shotgun sequence.
ACCESSION   DATCBB010000150 DATCBB010000000
VERSION     DATCBB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393971"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VII37_01740"
     CDS             complement(<1..1331)
                     /locus_tag="VII37_01740"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEY5068468.1"
                     /translation="MKNAILIADGAMGSMLHETVGTQRCFDELNTTEPEAVFRVHQAY
                     IQAGAQIIETNTFGANRFKLAPLGLGDEVQRLNSRGVKIAREARDSASSEVLVAGSMG
                     PLGLGVQSRHPEPDEIKEIFYEQALALEERGVDLYILETFTYIEELLIAVDAIRSFSG
                     LPIIAQLTYSEEGTTYGDLRPAAAAEQIKQKNVQVIGANCTLGPQALLPILQELAGVA
                     DLRVSGMPNAGIPKREGDRIVYPKSSPEYFALFAREAAAMGVRILGGCCGTTPAHIHA
                     MAEAVKSLRPVKAATVTHVAAPQPAAVAEREPESKFWRKLQNKEFGVCVEIDPPKGIS
                     LERVYEQVDKIMASGRVDAIDVNSGAMARVGMDALIVSGALEARGVETVPHLTTRDYN
                     IIGLQAMLLGAWTVGGVRNVLAITGDPPSVGDYPETSGVYEVDSVGLVKVL"
     gene            complement(1403..>2110)
                     /locus_tag="VII37_01745"
     CDS             complement(1403..>2110)
                     /locus_tag="VII37_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5068469.1"
                     /translation="VEGIGEDFFPSTMNLKILDDVLQVSDEECFVVARRLVKMEGLFT
                     GGSGGGCISATLRLAKDLGPEAFVVALLPDTGMRYLSKVYNDEWMRERGYVDAAVAIT
                     AAEVVHAKHATRKARELIIARPYQTVFHALKTMQEQDISQIPVFEENLPIGTIYEDQI
                     LNLALQGKDLRKLVVREMMSKPLPQIPGSAPVERITHILSHENPAVFVEMGNSRFEIL
                     TKYDLMSTVASLMEQKR"
BASE COUNT          445 a          725 c          480 g          460 t
ORIGIN      
        1 tgcaaaactt tcaccagccc cacggaatcg acctcataca ccccgctagt ctccggatag
       61 tcgccaaccg atggcgggtc gccggtgatc gccagcacgt tgcgtacacc gccaaccgtc
      121 cacgccccca gcagcatcgc ctgcaatcca ataatgttgt aatcccgggt ggtcaggtgc
      181 ggcacggttt ccacgccgcg agcctccagc gccccagata caatcaacgc atccatgcct
      241 acgcgcgcca tcgctccact gtttacatca atggcatcca cgcgccccga cgccatgatt
      301 ttgtccacct gctcgtaaac gcgctccagc gaaattccct ttggcggatc gatttccacg
      361 cacacgccga attctttgtt ctgcagtttc cgccagaact tgctctccgg ctcacgttcc
      421 gccaccgccg caggttgcgg cgccgccacg tgtgtaacgg ttgcggcctt caccgggcgc
      481 agcgacttta ccgcctcagc catcgcatga atgtgtgccg gcgtcgtccc gcagcatccg
      541 cccagaatcc gcacacccat cgccgcggcc tcgcgtgcaa acagcgcaaa atattccggc
      601 gaagatttgg gataaacaat gcggtcgcct tcccgcttgg gaatccccgc gttaggcatc
      661 ccgctgacgc gtaaatctgc aacccccgcc aattcctgca aaatcggcag caacgcctga
      721 ggacccagcg tgcaatttgc cccgatcacc tgaacgtttt tctgtttgat ctgctcggcc
      781 gccgccgccg gacgcaaatc cccatacgta gtgccttctt ccgagtacgt aagttgcgca
      841 atgatcggca aacctgaaaa agaccgtatc gcgtccaccg caatcaacag ctcttcgata
      901 taagtaaagg tttccagtat gtacaaatcg acgccacgct cttccaacgc cagcgcctgc
      961 tcgtaaaaaa tctccttgat ctcgtcaggt tccgggtgcc gcgattgcac ccccaacccc
     1021 agcggcccca tggaccccgc caccagcact tcactgctcg ccgaatctcg cgcttcgcgt
     1081 gcaatcttta cgccccggct gttcagccgc tgcacttcat cgccaagtcc cagcggcgcc
     1141 aacttgaatc ggttggctcc aaatgtattc gtctcgataa tttgcgcgcc ggcctgaatg
     1201 taagcctggt gcacgcgaaa aaccgcttcc ggctcggtcg tattcagctc atcgaaacag
     1261 cgctgcgttc ccaccgtttc atgcagcatc gaacccatcg ctccgtcagc aatgagaatc
     1321 gcgttcttca accgttcatt gaattcctga atccgcaatt agtttttcca gtgtctagaa
     1381 ttttaggaag agaaagacgc catcaacgct tttgttccat caggctggcg accgtgctca
     1441 ttaaatcgta cttcgtgagg atttcaaacc gcgagttacc catctccacg aacaccgccg
     1501 gattttcgtg gctcaaaatg tgcgtgatgc gttctactgg cgcgctccct ggaatctgcg
     1561 gcagcggctt gctcatcatt tcgcgcacca ccagcttgcg caaatccttg ccttgcagcg
     1621 ccaggtttaa aatttgatcc tcgtaaatcg tgccgatggg caaattctct tcgaacaccg
     1681 ggatctgcga aatatcctgc tcctgcatcg tcttcaacgc gtgaaacacc gtttgatacg
     1741 gccgcgcgat aatcagttcg cgcgctttgc gtgtcgcgtg cttggcgtgc accacttctg
     1801 ccgcggtgat ggccaccgcc gcgtccacat atccgcgctc gcgcatccat tcatcgttat
     1861 aaaccttgct caagtagcgc atgcccgtat ccggcaaaag cgctaccacg aaagcctccg
     1921 gccccaaatc cttggccagc cgcagcgtcg ccgaaatgca tccgccaccc gacccgccgg
     1981 taaacaatcc ctccatcttc accagccgcc gcgcgacaac aaagcattcc tcgtcgctca
     2041 cctgcagcac atcgtccaga atttttagat tcatagtgct agggaaaaaa tcctcgccaa
     2101 tgccttccac
//
LOCUS       DATCBB010000151         1074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_458916, whole genome shotgun sequence.
ACCESSION   DATCBB010000151 DATCBB010000000
VERSION     DATCBB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458916"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VII37_01750"
     CDS             complement(<1..293)
                     /locus_tag="VII37_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068470.1"
                     /translation="MAESSVSTGSSSSKSLLWMMIAMFVGIGGLLCGGLFMASRMVHS
                     VGLSAATNKDTIRTPAGSFRLEKQNEAGPTLAIYPNAILVLPGEKAAVIALQQR"
     gene            complement(431..>1074)
                     /locus_tag="VII37_01755"
     CDS             complement(431..>1074)
                     /locus_tag="VII37_01755"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEY5068471.1"
                     /translation="GGLLHAPDLYMEKIVVGPSCKGAMDLDAPVADNLKAIAKRLERD
                     VEDLVVIVLDRPRHEKLIADIRAAGARIRLIGDGDLSAGISAAVVGTGVHAVMGIGGA
                     PEGVLTAAALRCLNGEIVARLMVTKPEHAERLEKMGVKDPKRIYHTEDLAPGKKMIFA
                     CTGVTDGNLLRGVRFFGEGVRTQSMILTLDDRQVRFVDSVHLEKRSDIKVRFN"
BASE COUNT          211 a          356 c          282 g          225 t
ORIGIN      
        1 cgctgctgca gcgcaatcac cgcggctttc tcgcccggca gcaccagaat cgcgttgggg
       61 taaatcgcca acgtgggtcc cgcctcgttc tgtttttcca ggcgaaatga tccggcggga
      121 gttcgaatcg tatccttgtt cgtggctgcg gaaagcccta cgctgtgaac catccggctg
      181 gccatgaaga ggccgccaca cagcagtccg ccgattccca caaacatcgc gatcatcatc
      241 caaagtaatg atttggatga cgacgagccc gtcgataccg atgattccgc catcacattc
      301 tccgctggga aggcaatttg cccgattact attttggcag aaacagcctg tgccgacagg
      361 agtatgcccg gaagtgtgct ctaaacgtgc ctgttctttg gtgcaggccc ggttctggca
      421 agaacggcgc ctagttaaaa cgtactttaa tgtcggaacg cttttcgaga tgcacggagt
      481 ccacaaagcg cacctgccgg tcgtccagtg tcaggatcat cgactgggtg cgcacgcctt
      541 caccgaaaaa gcgcacgccg cgcagcaggt ttccgtcggt caccccggta cacgcgaaaa
      601 tcattttctt tccgggcgcc aggtcttccg tatggtaaat gcgcttggga tctttcacgc
      661 ccattttttc caggcgctcc gcgtgttccg gcttggtgac catcagccgc gccacaatct
      721 cgccgttcaa acagcgcaac gccgcggcgg tcaacactcc ttccggcgcg ccaccgatgc
      781 ccatcaccgc atgcaccccg gttcccacta ccgccgctga aatgcccgcc gacaagtcgc
      841 cgtcaccgat caagcggatg cgcgctcccg cggcgcgaat atccgcgatg agtttttcgt
      901 gccgcgggcg gtccagcacg attaccacca ggtcctcgac gtcccgctcc agtcgctttg
      961 caatcgcttt caggttgtcg gccaccggcg catccagatc cattgcaccc ttgcacgagg
     1021 gccccaccac aattttttcc atgtacagat cgggcgcatg cagcaagccg ccgc
//
LOCUS       DATCBB010000152         1044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_296822, whole genome shotgun sequence.
ACCESSION   DATCBB010000152 DATCBB010000000
VERSION     DATCBB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296822"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VII37_01760"
     CDS             <1..190
                     /locus_tag="VII37_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068472.1"
                     /translation="KKVMCNIVLTIVHEMTRTGNWMLRQSRASISQPLAWMRGSDPKA
                     ACEPARSREEQCFAGHVF"
     gene            236..970
                     /locus_tag="VII37_01765"
     CDS             236..970
                     /locus_tag="VII37_01765"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEY5068473.1"
                     /translation="MIDIHCHILPEVDDGPKSWEVAVEMCRMAMADGITHTVATPHAN
                     DRYAYDRAYLSGLLDQLREKVDPALQFSLGCDFHLSFENLERVLEHPHTYTIGETNYL
                     LVELSNYSVPAQISECFTRLGDRGLTPILTHPERNPILQQTPQRILEWAEQGCLIQVT
                     ASALTGFWGERPEIIARWLLDRSAVHFLSSDAHETKRRIPNLSAGRAVAEKIVGAEYA
                     TALVEGNPRAVVKGMPIPYSPRPVLD"
BASE COUNT          229 a          295 c          289 g          231 t
ORIGIN      
        1 gaaaaaagtt atgtgtaata tcgtgttaac tatcgtgcac gagatgacga gaactggcaa
       61 ctggatgctg cgccaatctc gagcctcgat ttcgcaacct ctcgcttgga tgcgtggaag
      121 cgaccccaag gctgcgtgcg aaccggcgcg aagccgcgag gaacaatgtt ttgcggggca
      181 tgtgttctaa ttgatagtgc tgaaaaaatg actattcgcg attaaagatt acaccttgat
      241 cgatatccac tgccacattc tgcctgaagt tgacgatggt cccaaatcct gggaagtagc
      301 cgtcgaaatg tgccgcatgg cgatggctga cggcatcacg cacaccgtcg ctactcccca
      361 cgccaacgat cgctacgcct atgatcgtgc ctacctttcc ggcctgctcg atcagttgcg
      421 ggaaaaagtt gatcccgcgc tccagttcag cctggggtgc gactttcatt tgtcgtttga
      481 aaacctggaa cgcgtgctgg agcatcccca cacctatacc attggcgaga cgaattatct
      541 gctggttgaa ctcagtaatt acagtgttcc ggcgcagatt tccgagtgtt tcacccgctt
      601 gggagaccgc ggtcttacgc cgatcctgac ccatccagaa cgcaatccga ttctgcaaca
      661 gacgccgcag cgcattttgg aatgggcgga gcagggctgt ttgattcagg tcacggcctc
      721 ggcgttgacc ggattctggg gagagcgtcc cgagatcatc gcccgctggc tgctcgaccg
      781 ctctgcggtg catttcctat cgtcggatgc gcatgagacg aagcgccgca ttccgaatct
      841 ttcagccggg cgagccgtgg cagaaaaaat tgttggagcc gaatatgcaa ccgcactggt
      901 tgagggtaac ccgcgcgccg tcgtgaaagg gatgccaatt ccctacagcc cacggccggt
      961 gttggattaa gcctaagccg ccgcaactcg caacgccttc gctggcgggc tagctaagca
     1021 gctacggcgc cagcagctat ttcc
//
LOCUS       DATCBB010000153         1267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_264509, whole genome shotgun sequence.
ACCESSION   DATCBB010000153 DATCBB010000000
VERSION     DATCBB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264509"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..274
                     /locus_tag="VII37_01770"
     CDS             14..274
                     /locus_tag="VII37_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HEY5068474.1"
                     /translation="MQKSALAFFILAAIASMSATPARAAIEYPWCAQYSGGEEGGGRN
                     CSFSTLEQCRATISGMGGFCEPNLFYPGSASDTSQRKRKRQH"
     gene            572..1195
                     /locus_tag="VII37_01775"
     CDS             572..1195
                     /locus_tag="VII37_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068475.1"
                     /translation="MTPEIKTFAVCTAIAAAIFTSGPAHASATCRTMAEARSAFPGAH
                     LYWHGNGHCWDNIGRTSGRASATQARGNIATTELPPPDVAPIVPNGNDVAPNPPPAIG
                     PIDPPPLIAMADGRLTVGAGIDDRIPTSKSLAPPLQEIIYSEDEYNFLDAQAAIQLAT
                     MQQKDHRMTIITALMLTFFIVFILSSVLIRRPFVRMNIENGPPIHRA"
BASE COUNT          300 a          377 c          330 g          260 t
ORIGIN      
        1 cggagcgtaa gccatgcaga aatcagcgtt agcgtttttc atcctggccg ccatcgcatc
       61 tatgtccgcc actccggcga gagcggcgat cgaatatccc tggtgcgccc aatatagtgg
      121 cggcgaagag ggcggcggca ggaattgcag cttctccacc ctcgagcaat gccgcgcgac
      181 catcagtggc atgggcggat tctgcgagcc gaacctgttc tacccagggt cggccagcga
      241 cacttcacag cgcaagcgca aacgtcagca ctagcatcgt acaggattgt gcggcaacac
      301 cacacggcga gcgtgcgcac gccgttggcg cgcatgttgg cgagggtcat gggggttgga
      361 gtttgcatcg agcaataagc ttagcagtgc ttgacgaccc gcgcatggtt atgaaagagg
      421 cgagcaagtg cgttaactcg tttttgccaa aatagtcaga attgatgggt ctaatgttct
      481 gatctgatgg aataactgcc ctaacttcaa aaaagcgaac tcacaattcc ttcacaccat
      541 tgcctgattt gctctattca cttcacgggc cgtgacaccc gaaataaaaa cgtttgctgt
      601 ctgcaccgcc atcgcggccg ccatcttcac cagcggcccg gcgcatgcat ccgccacctg
      661 ccgaaccatg gctgaggccc gcagcgcgtt ccccggcgcg catctctatt ggcacggcaa
      721 cggacactgc tgggacaaca ttggccgcac cagcggtcgt gctagcgcca cgcaggcaag
      781 gggtaatatc gcgaccacag aactaccgcc acctgatgtg gcgccgattg ttccaaatgg
      841 taatgacgta gcgccaaacc cgcccccagc cattggaccc atcgacccgc caccattgat
      901 tgccatggct gatggccggt taacggttgg ggcgggcatc gatgatcgga ttcctaccag
      961 caaatcgcta gcgccgccac tacaggaaat aatttacagc gaagacgaat acaatttttt
     1021 ggacgcgcag gccgctatcc aactggccac tatgcagcaa aaggaccatc gcatgacaat
     1081 tatcaccgcg ctgatgctaa cattctttat tgtctttatt ttaagcagtg tcctgatccg
     1141 tcgtcccttc gtccgaatga acatagaaaa cggcccgcca attcaccgcg cttaatcgag
     1201 ctataaaagt aagaggccgc caactgaggc gccctctcaa gcttggggtg ttttccaaac
     1261 catcaac
//
LOCUS       DATCBB010000154         1550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_199295, whole genome shotgun sequence.
ACCESSION   DATCBB010000154 DATCBB010000000
VERSION     DATCBB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199295"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1383)
                     /locus_tag="VII37_01780"
     CDS             complement(40..1383)
                     /locus_tag="VII37_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY5068476.1"
                     /translation="MATLDQKFSRLAALKPARPLAVRATTLREPCEEDTMARLLGAGV
                     STNRFGEHLAVRNWFSTPEFVAPHSTCLQLLCQQRDEVISRRTHSALKNPEKWLFLDT
                     ETTGVAGGTGTYAFLIGLAWWDAGGLQVEQLFMRDFAEEHSVLCQLAARLAERPVLVT
                     FNGKTFDWPLLENRFRMTRSIPVPSLAAHLDLLHPARALWKFRLGTVRLTDLEREVLN
                     PTLLGWCRQGDVSSKFIPQFYFDYLRGGPPEPLLGVARHNQMDLRGLGALFGKINALL
                     AEETNSDQEIHGLDLFGLSKFLHRRGDKGRAQVACAQAVDLGLPEEFHSRAQCDLALM
                     AKRKGEHQLAAELWNEILTYEIPPDSSDAMHACEQLAIHYEQRVKDYERALEFAHRAR
                     KLLRARATHASVSARGGFVRREDLSTKRIKRLEARLTKCAGGLLEVAEARATVAD"
BASE COUNT          349 a          467 c          418 g          316 t
ORIGIN      
        1 cccgcagaca caaccttgac cagccttccc ggtcgggatt caatccgcga cagtggcgcg
       61 ggcttcggcc acctccagta gtcctccggc gcactttgtt aatcgcgctt ccaggcgctt
      121 gattcgtttc gtcgacaaat cctcacgccg cacaaatccg cccctcgcgg acacgctcgc
      181 gtgggtagcc cgcgcgcgta gcagcttgcg agcgcggtgc gcaaattcca gcgctcgttc
      241 gtagtccttg acacgttgct catagtgaat ggccaattgc tcgcaggcgt gcatggcatc
      301 tgaggagtcg ggcggaatct cataggtcag aatttcattc caaagttccg ccgctagttg
      361 atgctcgcct tttcttttgg ccataagcgc caagtcgcat tgggctcgcg aatgaaactc
      421 ctccggcaga cccagatcga cggcttgagc gcaggcaacc tgcgcgcgtc ctttatcccc
      481 gcggcgatgc agaaactttg acaagccaaa aagatccaga ccgtgtatct cctgatcaga
      541 gttggtttcc tcagccagca acgcgttaat ttttccaaac agcgcgccca gtcctcgcag
      601 atccatttga ttgtggcgtg cgacccccaa caatggttcc ggcgggccgc cgcgaaggta
      661 atcgaaataa aattgcggaa taaatttcga ggagacgtca ccttgtctgc accatccaag
      721 cagcgttgga ttcaaaactt cgcgctccag atcggtcagg cgaacggttc caaggcgaaa
      781 tttccaaagc gcacgcgcag gatgaagcaa atccaagtga gcagccaggc tcggcacggg
      841 aatcgaacga gtcatcctga agcgattttc cagcaacggc caatcaaatg ttttcccgtt
      901 gaaggtcacc aacaccggtc gctcagcgag tcgtgcggca agctggcata aaaccgaatg
      961 ctcctcggca aaatcgcgca tgaataattg ctcgacctgc aaacctccgg catcccacca
     1021 agccaggcca atcaggaaag cgtaagttcc ggtcccgcca gcgactccgg tggtctcggt
     1081 gtctagaaaa agccacttct caggattttt cagcgccgag tgcgtgcggc gcgaaatcac
     1141 ttcgtcgcgt tgttggcaaa gaagctgcaa acaagtcgag tgaggagcaa caaattccgg
     1201 ggtagaaaac cagtttcgca cggcaagatg ttcgccaaaa cggttggtgg atactccggc
     1261 gccaaggagt cgcgccatag tgtcttcttc gcacggttct cggagcgtcg tagctcgtac
     1321 tgccaaaggg cgcgcaggct ttagtgctgc caggcgcgag aatttctgat cgagtgtggc
     1381 catctatcgg gcctcgccgt tcgaattgca caggcgctcc aaaatcgcca gcgcagcttc
     1441 cttggctcgc ggagtcaagt cccccgcggg ccccacacac gagggacagc ctgctgcgca
     1501 tgtacatgcg acgatcaact ctcgcgtccg cagcatcaga atgtcatgcg
//
LOCUS       DATCBB010000155         4587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_394142, whole genome shotgun sequence.
ACCESSION   DATCBB010000155 DATCBB010000000
VERSION     DATCBB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394142"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2358
                     /locus_tag="VII37_01785"
     CDS             <1..2358
                     /locus_tag="VII37_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY5068477.1"
                     /translation="HQLYDALNALRLDPASVYQIKPEHRIELRRGDAKISFDQGELIF
                     FAPFDNEITGAVFSGRGHILALPRDTIEKQQMARFLDSPILDQDFASAYIRFTDTSAA
                     DLLRQLQTAGLKPESDTASASRWDPVLSRLNATHSLRIFCGILLATPRPYFYASLDGI
                     ATGPFDVVLDSMRAEPFVLGQTRTVEGSTFYDVWASYKIPDTLPMAPDFRALHYSIET
                     SILPDHSLDATATVDLRALGAGQRFLIFELSHALNIESVSSGDGQKLEFFQNEDISPQ
                     ERSVRANDFLFVVLPRPAPRDSEFSIRFHYRGSIITDAGNDVFFVGARESWYPHFGDS
                     ADFAAYDLTMRWPKKLRLVATGAKIDEHEEADFRVGHWKTEKPVAVAGFNLGEYASSS
                     ISSANYSIDVYANHQVEQQLAKRLAANPDSELQHLGPRHPILDMNQDLQPLPANPAIA
                     LKQLGKDIDASIRFYETYSGPFPFHNLSVSQIPGTFGQGWPGLLYISTYSFLSADVQR
                     RAGLSSSGQEHFSELVPFHEVAHQWWGNIVGWSSYRDQWIDEAIANYLALLFADMHKN
                     PDHTLRVWLQRYRQMLIEKKPDADQPAGDIGALALGSRLNSSKSPNAYDDVIYAKGAW
                     VIHMLREMLRQPGANPDARFNALLRTLVTKYAYRALPTADLRREIEAVMIPSMDLEGG
                     HSMEWFFEQWVQGTGIPHYRADFTVHKSDNGAGYSMRGKLFQENVPQSFIARVPLYAA
                     SPGGHPVLLGTVVAAGPETQFNFTSSTSPHKLLIDAKMTLLCTTE"
     gene            complement(2452..3342)
                     /locus_tag="VII37_01790"
     CDS             complement(2452..3342)
                     /locus_tag="VII37_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068478.1"
                     /translation="MNLKSYIALLLLVPVGFLGVWRLQQRIDAQQSAVTSEDDQVMLR
                     SSKLVKALSLEYAPLVADIYWTRVVQYFGNKHAGKQVDLRLLWPLLDVTSVLDPHLMP
                     VYHFGSMFLSDAPPRGAGRPDLAAELIERGIRANPDQWRLYYDLGFVYYFDMKDYAKA
                     SQAFYEGSKNPQSLIWMKILAAKIAAEGNSAQTSIFLWKDVYDTATDPSIKENALNHL
                     QLLQAQEDCEHLDALVTEYEKRFGRRPARINELVQAGLLQGAPVDALGYPYTLSEEGK
                     AEINLDSPLLEKQIMAPKDK"
     gene            complement(3339..4115)
                     /locus_tag="VII37_01795"
     CDS             complement(3339..4115)
                     /locus_tag="VII37_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068479.1"
                     /translation="MTRVGTVALNTFREAVRDRVLYNLVFFALAMMAAAILVGQISID
                     IEQIVIVSLGLSAISIIGLLISVFIGVGLVSKEIDKRTLYALLAKPLRRWEFILGKFA
                     GLALTLTVNTAAMAAGLFLALFYVKHSLERGDAVVLVAAYFILLKLALVVALAMLFSC
                     FTTPLLAILFTAGLYVAGLFVHEMRELQAGLINPALSGLLRWISYVLPNFENFDVMGA
                     AAHGRAIPATLIAENTVYAALYCAIVLAVAMAIFSQRNLK"
     gene            complement(4112..>4587)
                     /locus_tag="VII37_01800"
     CDS             complement(4112..>4587)
                     /locus_tag="VII37_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068480.1"
                     /translation="VVLDEPMSGLDPVGRREVRDIILELKKQGKTVLFSTHILSDAEM
                     LCDRVGVIVGGKLQGVGAPSELVGLKSQGMEILFELPAGAPSGGELLAQATRTGDRYR
                     LQIEESGLYAAMERLGAAGTKILSVTQVKPSLEEYFMHLIEADRAQSAAVEVSAK"
BASE COUNT         1048 a         1504 c         1005 g         1030 t
ORIGIN      
        1 catcagctct acgatgccct caatgctctg cgcctcgacc ccgcttcggt gtatcaaatt
       61 aagcctgagc atcgcatcga gctccgtcgc ggtgatgcga aaatctcctt cgatcaaggt
      121 gagctgattt ttttcgctcc cttcgataac gaaatcaccg gcgcggtctt ttccggtcgc
      181 ggtcacattc tcgccctccc gcgcgacacc atcgaaaagc agcaaatggc tcgcttcctc
      241 gattccccta tcctcgacca ggatttcgcc agcgcctata tacgcttcac cgacacatct
      301 gctgccgacc tactgcgcca gcttcaaacc gcaggactca agccggaatc cgacaccgcc
      361 tccgcttccc gttgggaccc agttctctct cgcctcaacg ccactcactc cctgcgcatt
      421 ttttgcggta ttctgctcgc cacaccgcgg ccgtatttct acgcaagtct cgacggcatc
      481 gctaccggcc ctttcgacgt ggtcctggat tccatgcgcg ctgagccctt cgtcttaggg
      541 cagacgcgca ccgttgaggg ctcaactttc tacgatgttt gggcttccta caaaatcccg
      601 gacacccttc cgatggcgcc ggattttcgc gccctgcact actccatcga aacctccatc
      661 ctccccgatc actcgctcga cgctaccgcc accgtagatc tccgcgctct aggcgcaggc
      721 cagcgttttt taattttcga gttatcccac gcgttgaaca tcgaatccgt ttccagcgga
      781 gatggccaga agctggaatt ttttcaaaat gaagacatct ccccgcaaga gcgcagcgtg
      841 cgtgccaacg attttctgtt tgtcgtactt ccccgccccg cgccacgcga tagtgaattc
      901 tctattcgct ttcattatcg cggcagcatc atcaccgacg caggcaatga cgttttcttc
      961 gtcggcgctc gcgaaagctg gtacccacac tttggcgact ccgccgattt cgctgcgtac
     1021 gacttgacca tgcgctggcc aaaaaaattg cggctcgtcg ccaccggcgc aaaaatcgac
     1081 gagcacgaag aagctgactt ccgcgtggga cattggaaaa ctgagaaacc cgttgccgtc
     1141 gccggtttta acctcggcga atacgcctcc tcttccatca gctccgcgaa ttattccatc
     1201 gacgtctacg ccaatcacca agtggaacaa cagttggcta aacgtttagc cgccaatcct
     1261 gactctgaat tacaacactt gggacctcgt cacccaatcc tggatatgaa tcaagaccta
     1321 caacccttgc ccgctaaccc cgccatcgct ctcaaacaac ttggaaaaga catcgacgct
     1381 tccattcgct tttacgaaac ctacagtggc cctttccctt ttcacaatct cagcgtctcg
     1441 caaattccag gaacttttgg gcaaggctgg cccggcttac tctacatttc cacttattcg
     1501 tttttgtctg cggatgtgca gcgccgtgcc ggtctgtcca gcagtggtca agaacatttc
     1561 agcgagctcg tgcccttcca tgaagtcgct caccaatggt ggggaaacat tgtcggttgg
     1621 tcgagttatc gcgatcagtg gatcgacgaa gctatcgcca attatctcgc tctacttttc
     1681 gcggatatgc acaaaaatcc cgatcacact ttgcgcgtat ggctgcagcg ttaccgccag
     1741 atgcttatcg aaaaaaaacc tgatgccgat cagcccgccg gcgacatcgg cgcgctagcc
     1801 cttggctccc gtctcaattc ctcaaaatct cccaacgcct acgacgatgt catctacgct
     1861 aaaggcgcct gggtaattca catgcttcgc gaaatgctac gccaacccgg cgcgaatccc
     1921 gacgcgcgct tcaatgccct gctccgcacc ttagtcacaa aatatgccta ccgcgctctc
     1981 cccactgcgg atttgcgccg cgaaattgag gcagtgatga ttccgtcgat ggacctcgaa
     2041 ggcggccatt ccatggagtg gtttttcgag caatgggtcc aaggtaccgg cattcctcac
     2101 taccgcgcgg acttcaccgt tcacaaaagc gacaatggcg ccggctactc catgcgcggg
     2161 aaactctttc aagaaaacgt gccgcaatct tttatcgcca gagtcccgct atatgcagcc
     2221 agccccggcg ggcatccagt tctactaggc acagtagtcg ccgccggccc tgaaactcaa
     2281 tttaacttca cttcatcaac gtcaccgcac aaactcctca tcgacgcaaa aatgactctc
     2341 ctgtgcacca cagaatagta ggtgctcgcc gcatctgtct ccggcaactg ttggtgtgct
     2401 tatttgcgaa tttagtgtgg attttgcgaa tgctgcacta gggcttacgc actacttatc
     2461 ctttggggcc ataatctgct tctccaacag cggactatcc aaatttatct ccgcctttcc
     2521 ctcttccgac aaagtatacg gataacccag ggcatcaacg ggcgcgccct gcaacagccc
     2581 ggcttgcaca agttcattaa tccgcgccgg ccgccgccca aatcgctttt catactccgt
     2641 cacgagcgca tcaaggtgtt cgcaatcttc ctgggcctgc aacaactgta aatgattcaa
     2701 cgcattttct tttatcgacg ggtcagtggc tgtgtcgtac acgtctttcc acagaaaaat
     2761 agaagtctgc gccgaattac cttccgcggc gatcttcgca gccaatattt tcatccaaat
     2821 tagtgactgg ggatttttgc tgccttcgta gaacgcctgc gacgctttcg catagtcctt
     2881 catgtcgaag tagtagacga atcccaagtc gtaatacagg cgccattggt ccgggttggc
     2941 gcgaattccg cgctcgatca actccgcggc caaatcgggc cgcccagccc cacgtggcgg
     3001 agcatcactc aagaacatcg acccgaagtg ataaacgggc atcagatgtg gatcgagcac
     3061 actcgtcaca tccagcaacg gccaaagtag ccgcaaatcc acttgcttcc ctgcatgctt
     3121 gtttccgaaa tattgcacta cccgcgtcca ataaatatcc gccaccagcg gcgcatattc
     3181 cagactcaag gccttcacca gtttgctgga gcgcaacata acctggtcgt cttcgctcgt
     3241 aaccgcgctt tgttgcgcat caatgcgctg ctgcaagcgc cacacaccca ggaagcccac
     3301 aggcaccagc agtagtagcg cgatgtagct ctttaaattc atttcaggtt gcgctgcgaa
     3361 aagatcgcca tcgctaccgc caacacaatg gcgcaataaa gcgcggcata aaccgtattc
     3421 tcggcaatca gcgtcgccgg tattgcccgg ccgtgtgccg ccgcgcccat cacatcgaag
     3481 ttctcgaaat tcggcagcac ataagaaatc caccgcagca aaccgctcaa cgccggatta
     3541 atcaagcctg cctgcagttc acgcatctcg tgaacgaata atcccgcgac gtagagtccc
     3601 gccgtaaaca gaatcgccag caacggggtg gtaaaacacg aaaacagcat cgccagcgcc
     3661 accactaacg ccagcttcag caaaatgaaa tacgccgcca ctagcaccac cgcgtctccg
     3721 cgctccagcg aatgcttcac ataaaacaaa gccagaaaca ggccggcagc catcgcagcg
     3781 gtattcaccg tcaacgtcag cgccaacccc gcgaattttc ctaaaataaa ttcccatcgt
     3841 cgcaatggct tggccagcaa ggcatacaga gtccgcttgt cgatctcctt tgataccagg
     3901 cccacaccga taaataccga aatcagcaag ccaataatgg aaatcgcgct caatcccaga
     3961 ctgacaatca cgatttgttc tatgtcgatg gaaatctgcc cgacaagaat agccgccgcc
     4021 atcattgcca gcgcgaaaaa caccaggttg tacagcacgc gatcgcgcac cgcttcccga
     4081 aacgtattta gcgctactgt tcccacgcgt gtcactttgc gctcacttcc actgccgcgc
     4141 tttgtgcgcg gtcagcctct atcaaatgca tgaaatattc ttccagcgat ggctttacct
     4201 gcgtcaccga cagtatcttc gtgccggccg cgcccagccg ttccatcgcc gcatacaatc
     4261 cgctctcctc gatttgcagc cgatagcggt ccccggttcg cgtagcttgc gccagcaact
     4321 cccctccgct cggcgcgccc gccggcaact caaagagaat ttccattcct tgcgatttca
     4381 atcccacaag ttcactcggc gctccaactc cttgcaactt ccccccgaca atcacgccca
     4441 ctcgatcgca caacatttcc gcatccgaca aaatgtgcgt ggaaaataaa accgttttcc
     4501 cctgcttttt taattccagg ataatgtcgc gcacttcccg ccgccccacc gggtccagac
     4561 cggacatcgg ctcatccagc accacca
//
LOCUS       DATCBB010000156         1027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_426816, whole genome shotgun sequence.
ACCESSION   DATCBB010000156 DATCBB010000000
VERSION     DATCBB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426816"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1027
                     /locus_tag="VII37_01805"
     CDS             <1..>1027
                     /locus_tag="VII37_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY5068481.1"
                     /translation="EPKTIIANYTAMVGRTPLPAFWTLGFQQSRYSYYPEARAREIVQ
                     TYRQKKIPLDAIYFDIDYQLGNAPFTINRTYFPTFEKMIADFRGQGVRTVLITDLHVK
                     KDPNHNYKPYDSGTQRDVFVKNPDGSVFVGAVWPGESVFPDFTLAKARDWWGEQYKDF
                     TAMGIAGFWNDMNEPSVFLRADKTMPLDVLHRLDDGTTRDHRAIHNVFGKENVRATYE
                     GLRKLQPNERPFVLTRAAYSGTERYAATWTGDNSSTWNHLSMSTPMLLSLGICGYSLV
                     GDDIGGFAGTPTADLLTRWIEVGAFNPIYRDHTAKGTADQEPWVHGPEHEMIRRRYIE
                     LRYQLLP"
BASE COUNT          231 a          282 c          297 g          217 t
ORIGIN      
        1 cggagcccaa gacgatcatc gcgaattaca ccgcgatggt gggccgcacg ccgctgcccg
       61 cgttctggac gctgggcttt cagcaaagcc gctacagcta ctacccagaa gcgcgtgcga
      121 gagaaatcgt gcagacctac cggcagaaga aaataccgct ggacgcgatt tatttcgaca
      181 tcgactacca gctgggcaac gctcccttca ccattaaccg cacttatttc ccgacgtttg
      241 aaaaaatgat tgcggatttt cgcgggcagg gtgtgcgcac cgtactgatc accgacctgc
      301 acgtcaaaaa agatcccaat cacaactaca agccatacga ttccgggacg cagcgcgatg
      361 tattcgtcaa gaatcccgac ggcagcgtgt ttgttggtgc ggtgtggccg ggcgagagcg
      421 tgttcccgga ttttacgctt gcgaaagcgc gggactggtg gggcgagcaa tacaaagatt
      481 tcaccgcgat gggaatcgcg ggtttctgga acgacatgaa tgagccttcc gtttttctgc
      541 gcgcggacaa gaccatgccg ctcgatgtgc tgcatcgcct ggatgacggc actacgcgcg
      601 accatcgagc gattcacaac gttttcggca aggaaaatgt tcgcgccacg tatgagggct
      661 tgcgcaagtt acagccaaat gagcggccat ttgtcctgac gcgcgcggct tattccggca
      721 cggagcgcta tgcggcgacc tggacgggag ataacagcag cacgtggaat catttgtcga
      781 tgagcacgcc gatgcttctc agcttgggga tttgcggcta ttcgctggtg ggagacgaca
      841 tcggcggttt tgcgggaact cctacagccg atcttctgac gcggtggatc gaagttggcg
      901 cgtttaatcc tatttatcgt gaccatacgg caaagggcac cgccgaccag gaaccctggg
      961 ttcatggtcc ggagcacgaa atgattcgcc gccgctatat cgagctgcga tatcaattac
     1021 ttcctta
//
LOCUS       DATCBB010000157         1766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_69333, whole genome shotgun sequence.
ACCESSION   DATCBB010000157 DATCBB010000000
VERSION     DATCBB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69333"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VII37_01810"
     CDS             <1..526
                     /locus_tag="VII37_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY5068482.1"
                     /translation="QLCAGQGTVEGSRVQHGNPRGSEITNKRQTMVLITRRIEFSASH
                     VYHNAQLSPEENRRIFGKCNNPHGHGHNYTLEVTVAGEPDPVTGMVLDLKELKEILER
                     EVMQRMDHRHLNHEVPELAAQIPTCENIARVIWNLLEPKIKQGALHHVRLYESPDLFA
                     DCFRNGNAGHRGDL"
     gene            526..1008
                     /locus_tag="VII37_01815"
     CDS             526..1008
                     /locus_tag="VII37_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY5068483.1"
                     /translation="MNGSPNSAELKIELGRRYHFSASHRLHSVHLSEEENCRVFGKCN
                     NPFGHGHNYVLEVSISGRVGPATGMIANLGDLDAFVEKAVLQVFDHKSLNEDVAVFQE
                     IVPTTENLCIDIYERLKSFPHAKLERVRIEETRNNSFEYAGEELARVPVASRAEKERR
                     "
     gene            1032..1619
                     /gene="folE"
                     /locus_tag="VII37_01820"
     CDS             1032..1619
                     /gene="folE"
                     /locus_tag="VII37_01820"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY5068484.1"
                     /translation="MHEPKANAKEENVAALVRKMISIIGEDPNREGLRKTPERFEKAL
                     KFLTSGYHQNVDHILNGATFSVAYDQMVIVKDIEFFSLCEHHLLPFFGKCHVAYLPNK
                     KVIGLSKVARLVNMFARRLQIQERLTSQIAQAIEEKISPEGVGVIIEARHLCMQMRGV
                     EKQHGQAVTSAMLGAFRHNKETRDEFLALTRKRQS"
BASE COUNT          478 a          405 c          527 g          356 t
ORIGIN      
        1 gcaattatgc gcggggcaag gtacagtgga agggtcgcga gtacagcacg gaaacccgcg
       61 agggtcagaa ataacaaaca aaagacaaac tatggtgctg attacacgca gaatcgaatt
      121 ttcagcttcg cacgtttatc ataatgcaca gctttcgccg gaagagaatc gacgcatttt
      181 tgggaaatgc aataatccgc acggccacgg acacaattac acgctggaag ttacggtggc
      241 gggcgaacca gacccggtga ccgggatggt gttggatttg aaggaactga aagagattct
      301 ggagcgcgaa gtaatgcagc gcatggatca ccggcacctg aatcacgagg ttcctgaact
      361 agccgcgcag attcccactt gcgaaaatat cgctcgagtg atctggaatt tattggagcc
      421 aaagataaaa caaggcgcgt tgcaccacgt gcgactgtat gagtcgccgg atttgttcgc
      481 ggactgcttt cgaaatggga atgcggggca tcggggagat ttgtgatgaa tgggtcgccc
      541 aattcggcgg aactgaagat cgaattgggg cggcgctacc atttttcggc gtcccatcgg
      601 ctgcacagcg tgcatctcag tgaagaggag aattgccggg tcttcggcaa gtgtaataac
      661 ccgtttgggc acggacataa ttacgtgctg gaagtcagca tttcaggcag agtgggtccc
      721 gccacgggaa tgattgctaa cctgggcgat ctggacgcgt ttgtggagaa agcggtgctg
      781 caagtgttcg atcataaatc gctgaacgaa gacgtggcgg tttttcagga aatagtgccg
      841 accacggaaa atttgtgcat cgatatttac gaacggttga agagtttccc gcacgccaag
      901 ctggaacgcg tgcgcatcga agagacccgc aacaatagtt ttgagtatgc gggcgaagag
      961 ttggcgcgag tccccgtcgc gagtcgtgcg gaaaaggagc gaaggtgagc gaacctggcg
     1021 aagagctatt gctgcacgaa ccgaaagcca acgccaaaga agaaaatgtc gccgcgctgg
     1081 tgcgcaagat gatctccatc attggagaag accccaaccg cgagggcctg cgtaagacgc
     1141 cagagcgatt tgagaaggcg ctgaaatttt tgactagcgg ctatcaccag aacgttgacc
     1201 acattttgaa tggcgccacc ttcagcgtgg cctacgacca gatggtcatc gtgaaggaca
     1261 ttgaattttt tagcttgtgc gagcatcact tgctgccgtt tttcgggaaa tgtcacgtgg
     1321 cgtacctgcc gaataaaaaa gtcattgggt tgagcaaagt ggcgcggctc gtaaatatgt
     1381 ttgcgcgaag attacagatt caagagcgcc tgaccagcca gatcgcgcag gccatcgaag
     1441 aaaaaatcag tcccgaaggc gtgggagtga tcatcgaggc ccgccactta tgcatgcaga
     1501 tgcgcggcgt ggaaaaacag catggccagg cagtaaccag cgcgatgctg ggtgcgtttc
     1561 gtcacaacaa ggaaacgcga gacgagtttt tggcgctgac gcgaaaacgc caatcctgac
     1621 cttaaccaat aagaatatcc ggacagcatt actgccgcgc ccaggcacgg cagagcaaat
     1681 tgatttattc gaaaggaacg gctgatggct gaacaaaata agggcaagat cgcgctggtg
     1741 acgggtggaa atcgcggaat tggact
//
LOCUS       DATCBB010000158         1072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_102505, whole genome shotgun sequence.
ACCESSION   DATCBB010000158 DATCBB010000000
VERSION     DATCBB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102505"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1072)
                     /locus_tag="VII37_01825"
     CDS             complement(<1..>1072)
                     /locus_tag="VII37_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068485.1"
                     /translation="LPGLLATLVNVFRKPADLPWALHLRSVAGSGGRQLGQIILTLAF
                     LPYDAFISLDAIGRTLVRLLVTRKRLLEWQTSGDSERTTRADLAGFYATMCIAPMVAL
                     AMGIFLVIMLPSQLSLALPILALWLAAPLIAWWISQPIESPASDLTTGQLTFLRHTAR
                     KTWHFFETFVTAQENWLPPDNFQETPSPTIASRTSPTNMGLALLANLAARDFGYLPVS
                     GVIRRTQDAFATMQKLEKYNGHFYNWYETRTLRPLLPLYVSSVDSGNLAGHLLTLGSG
                     LRELADEKIFTPQIFAGLRDTVGILKTLAVENVALSQLDAALEQAPSTLRPAHALLER
                     ATGLATQIAASFADKADELKTWS"
BASE COUNT          235 a          316 c          321 g          200 t
ORIGIN      
        1 actccacgtt ttcagttcgt ccgctttgtc tgcgaacgaa gcggcaatct gggtcgccag
       61 gcccgtcgcg cgttccaaca aagcatgcgc cgggcgcagc gttgacggcg cttgctccaa
      121 tgctgcgtcg agctgcgaca atgccacgtt ttcgacagcc agtgttttca agatgcccac
      181 cgtgtcgcgc agcccggcga atatctgcgg tgtgaaaatt ttctcatccg ccaattcccg
      241 caacccagag ccgagcgtca gcaaatgtcc cgcaagattt ccgctgtcca cactggaaac
      301 gtagagcggg agcagtggtc gcagtgtgcg cgtttcatac caattgtaga aatgcccgtt
      361 atacttttcg agtttttgca tcgtggcgaa ggcgtcttgc gtgcggcgaa tcacgccgct
      421 gaccggcaga taaccaaaat cacgcgcggc cagattggcg agcaacgcca gacccatgtt
      481 cgtcggcgag gtgcgcgaag cgatcgtcgg cgagggtgtt tcctgaaaat tatccggcgg
      541 cagccagttc tcctgtgcgg tgacaaaggt ctcaaaaaaa tgccaggtct tccgcgcggt
      601 gtggcggaga aacgtcaatt gcccggtcgt caaatccgac gcaggcgatt cgatgggctg
      661 gctgatccac cacgcgatca acggagcggc gagccagaga gcaaggattg gcagggccag
      721 agacaattgg gacggcagca tgatgacgag gaaaattccc atcgccagcg cgaccatcgg
      781 cgcgatgcac atcgtggcat aaaagccggc gaggtcggcg cgcgttgtcc gttcggaatc
      841 acccgacgtt tgccattcga gaagtcgttt gcgcgtcacc agcaaacgca ccagcgtccg
      901 gccgatggcg tccaagctga tgaaagcgtc gtagggtaaa aaagccagcg tgagaatgat
      961 ctggccgagc tgccgtccgc ccgatccggc cacgctccgc aggtgcaacg cccacggcaa
     1021 atccgctggc ttgcgaaaca cattgaccag cgtcgccagc aatccgggca gt
//
LOCUS       DATCBB010000159         2092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_297060, whole genome shotgun sequence.
ACCESSION   DATCBB010000159 DATCBB010000000
VERSION     DATCBB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297060"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VII37_01830"
     CDS             <1..801
                     /locus_tag="VII37_01830"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5068486.1"
                     /translation="LEGHRQRDEEEGQTFTKMVGQEVLPKFLSVTDDPTTKQIGDVKL
                     SGSYDFDNEGTPAQRVDVIENGVLKNFLMSRMPIKNFGVSNGHGRNQPGLMPTGRQGN
                     LIVTSSQTVPEKEMRQKLIDEVKKQGKPYGLYFDDIQGGFTLTTRSLPQAFQVLPVIV
                     YKVYVDGRPDELVRGVDIVGTPLAALTRIVATGDQEHVFNGICGAESGSVPVAAVAPA
                     IIFSEMEVQKRAHAHERPPILPPPGFEDAAPAVAAQSVAPVAGAGARP"
     gene            798..>2092
                     /locus_tag="VII37_01835"
     CDS             798..>2092
                     /locus_tag="VII37_01835"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5068487.1"
                     /translation="MKIICLALLSGILFFVGPTFGAPVTGSGDTANNSPTAESARPLN
                     SSSMAAAVAAAKGDALLEALLIELSRSYTHLKMDQVAAPYYIEYRVNDVDDYTAEAAF
                     GALVQSQRMHVRILRVVVRIGDYKQDSYFSQGVGETNILPLDNDPIALRHQIWTATDE
                     AYKSAGEAFAEKQAALKQFSADPNAVDDFAKEPEVIALEPVVTLKVDQKEWKKTLEDV
                     TELYKQYPDVQSSTASARFTGVNEYFVNSEGTLTRDGRATYSVQLNSAAQAEDGMRVT
                     RNPAWMVARAEELPSRVALVSDAKKTLETVVALRNAPIVEEEYRGPVLFAPDAADDIV
                     ASLIGQNVVGRKPQLGKPNRTTGSFASSYKTRVLPSFLSVVDDPTLKEFQGKSTVGSY
                     DVDSEGVKAQRVTVIDNGTLTNYLVGRQPIRDFTASNGHG"
BASE COUNT          498 a          444 c          713 g          437 t
ORIGIN      
        1 ttagaaggac accggcagcg agatgaggaa gaagggcaga catttacgaa gatggtggga
       61 caggaagtac tgccgaaatt tcttagcgtg acggacgatc cgacgacgaa gcagatcggg
      121 gatgtgaaac tttcggggtc gtatgatttt gacaatgagg ggacaccggc acagcgtgtc
      181 gatgtaattg aaaatggcgt tttgaagaat tttttgatgt cgcgcatgcc gatcaagaat
      241 ttcggagttt ccaacgggca tggacgaaac cagccggggt tgatgcccac tgggcgacaa
      301 ggaaatttga ttgtgacatc cagccagacg gttcctgaga aggagatgcg gcagaagttg
      361 atcgatgaag tgaagaagca ggggaagcct tacggactgt acttcgatga catacaaggc
      421 ggatttacac tgacgacgcg gtctttgcca caagcctttc aagtgcttcc ggtgattgtt
      481 tacaaggttt atgtcgatgg gcggccggat gagttggtgc gcggagtgga tattgtgggc
      541 acgccgctgg cggcgctgac gcgcattgtg gctaccgggg accaggaaca tgtgttcaac
      601 gggatctgcg gcgcggaaag tgggagcgtg ccagtagcgg cggtggctcc cgcgattatt
      661 ttttcggaga tggaagtgca gaagcgagcg catgcgcatg agcggccgcc cattctgccg
      721 ccaccgggat ttgaagacgc ggcacctgct gttgcggcgc aaagcgtggc acccgttgct
      781 ggcgcggggg cgagaccatg aagataattt gtctagcttt gctcagcggg attttatttt
      841 ttgtggggcc aacctttggg gcaccggtta ccggaagtgg agacacagcg aataattcac
      901 cgactgccga aagtgcacgg cctctaaaca gcagcagcat ggcggcggct gtagcagcgg
      961 caaagggcga tgcgctgctt gaggcgctgc ttatcgaact gtcgcggtcg tacacccatt
     1021 tgaaaatgga tcaggtggcg gcgccttact acatcgaata tcgcgtgaac gatgtagatg
     1081 attatacggc ggaagcggct tttggggcgc tggtacagag ccagcggatg cacgtgcgga
     1141 tattgcgggt ggtggtgcgc atcggagatt acaaacagga tagctatttc agccagggcg
     1201 ttggagagac gaatattttg cctctggata acgaccccat cgctttgcgt caccagatct
     1261 ggacggcgac ggatgaggcg tataaaagcg cgggagaggc gttcgcggaa aagcaggcgg
     1321 cgctgaaaca gttcagcgcg gatccgaacg ccgtcgatga ttttgccaaa gagccggaag
     1381 tgattgcgct ggaaccggtg gtgacattaa aggtcgatca aaaggagtgg aagaagacgc
     1441 tcgaagatgt gacggagcta tataaacagt atccggatgt gcagagttcg acggcgtcgg
     1501 cgcggttcac gggagtgaac gaatattttg tgaattcgga agggaccttg acgcgggatg
     1561 gtcgggcgac gtatagcgtg caattgaaca gtgctgcgca ggcagaagat gggatgcgcg
     1621 tgacgcgcaa tccggcgtgg atggtggcac gtgcggagga gttaccgagc cgcgttgcct
     1681 tagtgagcga tgcgaaaaaa acattggaga cggtggtggc gttgaggaat gcgccgatcg
     1741 tggaagagga ataccgtggt ccggttttgt ttgcgccgga cgccgcggat gacattgtag
     1801 cttcgctgat tgggcagaac gttgtgggac gcaagccgca acttggtaag ccaaatcgta
     1861 caaccgggtc gtttgcgtcg agttataaga cacgggtgct accaagcttt ttgagtgtgg
     1921 ttgacgatcc gacgctgaag gaatttcagg gcaagagcac ggtgggaagc tacgacgtgg
     1981 acagcgaagg cgtgaaagca cagcgcgtga cggtgattga taacggaaca ttgaccaatt
     2041 atttggtggg ccgccaaccg atacgtgatt ttacggcttc gaatgggcat gg
//
LOCUS       DATCBB010000160         1276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_297065, whole genome shotgun sequence.
ACCESSION   DATCBB010000160 DATCBB010000000
VERSION     DATCBB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297065"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VII37_01840"
     CDS             <1..314
                     /locus_tag="VII37_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068488.1"
                     /translation="CPDSGEADTFEMRNLEALSTNDEIKFVLSTRRDYEFAREFIVQH
                     NLRERVHQVLFSPVFEGPEGKWPGLEARKLVEWMLEDSLQVRLGLQLHKFVWDPATKG
                     V"
     gene            318..1043
                     /gene="queC"
                     /locus_tag="VII37_01845"
     CDS             318..1043
                     /gene="queC"
                     /locus_tag="VII37_01845"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY5068489.1"
                     /translation="MPDSKAQNVKAVVLLSGGMDSCVCTAIAVQAHGAERVALLHAGY
                     GQRTQKRERQAFESIADFYHVQQRLVVQLDHFRAIGGSALTDGSIAVPEHQEISDSTS
                     TGEIPVTYVPFRNAHFLSVAVSWAEVIGANSIYIGAVAEDSSGYPDCRPEYYRVFQEL
                     IRVGTRPKTHIEIATPVIALKKSEIIRKGIELGAPLHLTWSCYQSENVACGVCDSCLL
                     RARAFAEAGASDPISQRGAAAHS"
BASE COUNT          274 a          332 c          382 g          288 t
ORIGIN      
        1 aatgcccgga ttcaggcgag gcagatacct ttgagatgcg taacctagaa gctctgagca
       61 caaacgacga gataaaattc gtgctttcca cacggcgcga ttatgagttc gcgcgcgagt
      121 ttattgtgca gcacaatttg cgtgaacgcg tccaccaagt tttgttctca ccggttttcg
      181 aaggtccgga aggcaaatgg ccgggcctgg aggctcgcaa attagtggag tggatgctcg
      241 aggattctct gcaagtgcga ttgggattac agttgcacaa gttcgtatgg gacccggcaa
      301 cgaaaggcgt ttgaaatatg cctgactcaa aggctcaaaa tgtgaaagca gtggtgctgc
      361 tgagcggtgg catggattcg tgcgtgtgca ctgcgatagc ggtacaggca catggggcgg
      421 agcgtgtggc gctgctgcac gccggttacg gacagcggac gcaaaagcgt gaacggcagg
      481 cattcgaatc catcgcggat ttttatcatg tgcagcaacg gctggttgtt caattggatc
      541 attttcgagc cattggcggc tcggccctta ccgatggcag catcgccgtc ccggagcatc
      601 aggagatttc tgacagcact agcaccggcg aaatacccgt cacttacgtc ccttttcgca
      661 acgcgcattt cctttccgta gcggtaagct gggctgaagt tatcggcgcg aactccatct
      721 acatcggtgc ggtcgcggaa gatagctccg gctatcccga ttgccgcccc gaatattatc
      781 gcgtctttca ggaactgatt cgcgtgggta ctcgtcccaa gacgcatatt gaaatcgcaa
      841 cgcctgtgat cgccttgaag aaaagcgaaa tcattcgcaa gggcatcgag ctcggcgctc
      901 cgctgcatct tacctggtcg tgttaccaaa gcgagaatgt cgcgtgcggg gtatgcgata
      961 gttgcctgct gcgtgcgcgt gcgttcgcgg aggccggcgc aagcgacccc atttctcagc
     1021 gtggtgcggc agcccattcc tgacctttga atccagcacc gaatccatga taatggtgcg
     1081 cgcctcgccc gggccgtatg cagttgcaaa ggagaatcaa ccatgaagag atacgcgagt
     1141 tcgtggctgt tcagcgtctg cagcgtagcg ctgttactgg cagtgttagt tccgcgcgcc
     1201 gctgcgcaaa gcgatggctc ggttcgtgga caaatcctgg atgtcactgg caagccttgg
     1261 gctgacatgg gcatgc
//
LOCUS       DATCBB010000161         1939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_361679, whole genome shotgun sequence.
ACCESSION   DATCBB010000161 DATCBB010000000
VERSION     DATCBB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361679"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..>1939
                     /locus_tag="VII37_01850"
     CDS             247..>1939
                     /locus_tag="VII37_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY5068490.1"
                     /translation="MRTTLKIVLPLIISVAVISLLFAAYQVRTERRILRNDLSRRAEI
                     LGESLQESVEPLLDLGREKNLQRLVDRFGQREHLKGVAVYNTSGSALAITPALAPVFK
                     SRPATATHAAEKDSGYGQFLKIDGQPMHIYALPLHSSAKPAGVLALYHDTTYIDVQLS
                     HTLRDALLTALIQTLLITALAMVLVRWTFTNPLTRTAKWLRTLRTGQSHAPPTLPEGE
                     IFDQLHHEVTHLARDLNAARASAEKEARLRDTNASQWTSERLRVSLRNKLKGQTLFVV
                     SNREPYMHVINENRSVSVIVPASGLVTALEPVLLACDGTWIAHGAGSGDRDTVDSHDR
                     LRVPPDRPSYNLRRVWLTVEEEKGYYQGFANEGLWPLCHIAHTRPIFRPEDWLYYQQA
                     NRDFANAVLDEMEGTESPILLAQDYHFALLPRMVKEARPDARVAIFWHIPWPNPEVFG
                     ICPWQRELVDGLLGADLIGFHTQSHCNNFLSTVDRAVEALTEWDRFAVNRQGHVTRVR
                     PYPISVAFPEHTRAINESRNAGEERAALCAELGIEASLLGVGVDRVDYTKGIIERF"
BASE COUNT          411 a          529 c          581 g          418 t
ORIGIN      
        1 ggcgcggtaa tggcgttaga ggcaaacgcg aaagcctcct gaggaatagc gccccgctgt
       61 ggaaaaattt ggcgctcggt gaccaactga cacacatgaa ttgcgcacgg gcccgacgat
      121 gacgccgtgc accatgcctc gcttttggat taagatactt gtcccagcgc tggatgatgc
      181 cgtggccgcg attgttgcgg ccaaccagat cggcgcgcgc atttcgcacc cgggtgagga
      241 gccagtttgc gcacgacgct gaagattgtc ctgcctttaa ttatcagcgt agctgtcatt
      301 tccctgctgt ttgccgctta ccaggtgcgc accgaacggc gcatcttacg caacgatctt
      361 tcccgccgcg cggaaatcct tggcgagagc ctgcaagaaa gcgtcgagcc tttgctggac
      421 ctcgggcggg agaaaaatct gcaacggctg gtggaccgct tcggccagcg cgaacatttg
      481 aagggcgtgg ctgtctataa cacgagcggt agcgccctgg ccatcacccc ggcgttggcg
      541 ccagttttta aatcgcggcc ggcgaccgct acacatgcgg cagaaaaaga ttctggttat
      601 ggacagtttt taaaaattga tgggcagccg atgcatattt acgcgctgcc tttgcatagc
      661 agtgctaaac cggcgggtgt tttggcgctg tatcacgaca ccacctacat cgacgtacag
      721 ctttcgcaca cgctacgaga cgccctgctc acagcgctaa ttcaaacgct gctgattact
      781 gcgctggcga tggtgctggt gcggtggacg ttcaccaatc cattgacacg taccgcgaag
      841 tggttgcgta cgctgcgcac cgggcaatcg cacgcgccgc caacgttgcc ggaaggtgaa
      901 atattcgacc aactgcacca cgaagtaacg cacctagcac gtgatttaaa tgccgcgcgc
      961 gcttccgccg aaaaagaggc gcgattacgc gacacgaatg cgtcgcagtg gacttctgag
     1021 cgcctgcggg taagtttacg aaacaaattg aagggccaaa cgcttttcgt ggtgtctaat
     1081 cgcgaaccat atatgcacgt gatcaacgaa aatcgttccg tgagcgtaat cgttccggcc
     1141 agtggattgg ttaccgcgtt ggaacccgtg ctgttggcgt gtgatggaac gtggatcgcg
     1201 cacggggcag gcagcggaga tcgcgacacc gtggattcgc atgatcgctt gcgcgtcccg
     1261 ccggaccgtc cgagttataa cctgcggcgc gtatggctga cggtggaaga agagaaaggt
     1321 tactaccagg gcttcgccaa tgaagggctg tggcctcttt gccatatcgc gcatactcgc
     1381 ccgatttttc ggcctgaaga ctggctgtac taccagcagg ccaatcggga ctttgctaat
     1441 gcggtgctcg atgaaatgga aggcacggaa tcaccaatcc tgctagcaca ggattatcac
     1501 ttcgcgttgc tgccgcgaat ggtgaaggag gcgcgaccgg atgcgcgtgt ggcaattttc
     1561 tggcatatcc catggccgaa tccggaagtg ttcggcattt gtccctggca gcgggagctg
     1621 gtcgatggct tactgggcgc ggatctcatc ggttttcaca cccaatcgca ttgcaataat
     1681 tttctttcga cggttgatcg cgcggtggaa gcgctgaccg aatgggaccg ctttgcggtg
     1741 aaccgccagg gtcatgttac gcgcgtgcgc ccgtatccca tcagcgtggc ctttcctgag
     1801 catacccggg ccatcaatga atcgcgcaat gccggtgaag aacgcgcggc actgtgcgcg
     1861 gagctgggca ttgaagcaag tttgctcggg gtgggtgtag atcgcgtgga ttacaccaaa
     1921 ggaattatcg agcgctttc
//
LOCUS       DATCBB010000162         1532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_102607, whole genome shotgun sequence.
ACCESSION   DATCBB010000162 DATCBB010000000
VERSION     DATCBB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102607"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VII37_01855"
     CDS             complement(<1..1521)
                     /locus_tag="VII37_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5068491.1"
                     /translation="MAKAGKKVLVLERRHVLGGAAVTEEIIPGFLFSECSYVVSLLRP
                     EIIRELDLPRHGLEILPLDGTFSPMPDGNHLWRMNDHAKSIREIRRHSRVDAEAYDEF
                     SKMMTPMCRFVKPMLSMVPPDPTTLNPRDLKQLHFLMQRFRELSSDERYTLIQLMTMS
                     SADFLDQWFETDVLKATMSASGIIGTFLGIRSPGTAYVLLHHYMGEIDGAFRSWGFSR
                     GGTGAISNAIAAAAREAGVEIRTKAPVGKILVKEGRAAGVVLQSGEEITANVISSSVD
                     PHLTFEKFLEPAELPADFLEGVRRYKFRGSSGKVNIALDALPEFKSLPGDGAHLRGAI
                     SISPSMEYMERAYDDAKYGHYSRRPYIDMVIPSLTDPSVAPPGKHVLSCFVQYAPYKL
                     AEGTWDDQREAFGDNVIDTISEYAPNIKDIIVGRQILTPLDLEREFGLTQGNIFQGEL
                     SLEQLFFLRPVAGWAYYRTPVDNLYMCGSATHPGGGIMGANGRIASQVILREWKKAA"
BASE COUNT          373 a          407 c          422 g          330 t
ORIGIN      
        1 ggcggccttt ttccattccc taagaatgac ctggctggcg atgcggccgt tagcgcccat
       61 gatgccgccg cccggatgcg ttgccgatcc acacatataa agattatcga ccggagtgcg
      121 atagtaagcc caacccgcga cgggacgcag aaaaaataat tgctccagtg aaagttcgcc
      181 ctgaaaaata ttcccttggg tcagaccaaa ttcgcgttcc agatcgaggg gcgtaagaat
      241 ctgccgccca acgataatgt ctttaatgtt gggcgcatat tctgagatcg tgtcgatcac
      301 attgtctccg aaagcttcgc gttgatcgtc ccacgtgccc tccgcaagct tgtaaggagc
      361 gtattgcacg aaacacgaaa gaacatgttt gccaggaggc gcaacgctcg gatcggtcaa
      421 gcttggaatc accatgtcaa tgtagggacg gcgcgaatag tgaccgtact tggcgtcgtc
      481 gtaagcgcgc tccatgtatt ccatactggg agaaatagaa atcgcgccgc gaagatgcgc
      541 tccgtcgccg ggcagagatt tgaattcggg cagcgcatcg agcgcaatat tcactttgcc
      601 agatgagcca cggaatttgt agcgccgcac gccttccaga aaatctgccg gtaactccgc
      661 aggttcaaga aatttttcga aggtgagatg cggatcaacg ctggaagata ttacgttggc
      721 ggtaatctct tcgccggatt gcagaacgac tcccgccgcg cgcccttctt tcaccaatat
      781 tttccctact ggcgctttgg tgcgaatttc gaccccagct tcacgtgcgg ctgcagcgat
      841 ggcgttggaa atcgctccgg ttcctccacg gctgaatccc caggaacgaa acgctccatc
      901 aatttcgccc atatagtggt gcagaagcac gtacgcagtt ccaggcgagc ggatgcccag
      961 aaaagtgccg atgattccgg acgccgacat ggtggctttg agtacgtcgg tttcaaacca
     1021 ctggtccaga aaatccgcgg aactcatggt catgagctga atgagcgtat agcgttcgtc
     1081 ggaagatagt tcgcggaagc gttgcatcaa aaaatgcagc tgcttcagat cgcgcggatt
     1141 caacgtcgtg ggatcgggcg gcaccataga aagcatgggc ttcacgaaac ggcacatcgg
     1201 cgtcatcatc ttcgagaatt cgtcgtacgc ttcggcgtca acacgcgagt gccggcgaat
     1261 ttcgcggatg gatttggcgt gatcgttcat gcgccacaga tgattgccat caggcatggg
     1321 cgagaatgtg ccatccagcg gaagaatttc aagaccgtgg cgaggaagat caagttcacg
     1381 aatgatttcg ggccgcaaca gggagaccac ataggagcac tcggagaaaa gaaagcctgg
     1441 aataatttct tcagtcaccg cggcgccgcc cagcacgtgg cgacgttcga ggaccaaaac
     1501 tttttttcct gccttcgcca gataagcagc gt
//
LOCUS       DATCBB010000163         1059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_199521, whole genome shotgun sequence.
ACCESSION   DATCBB010000163 DATCBB010000000
VERSION     DATCBB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199521"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..>1059
                     /locus_tag="VII37_01860"
     CDS             94..>1059
                     /locus_tag="VII37_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068492.1"
                     /translation="MEIMKVIRYGKYVGEPADAVDLDELVKRMGDFFLQSGFESQYYG
                     VSEMDNQRSMEALKEAILRALQEGDLLPEDAMSEELRKMLQSPQAQNSQAVKDLLEKL
                     MDRLAQEGFINPQQPPQVTPPHQNTARGSLGQPQERQTEARFEITDKTIDFLGFKTLQ
                     DLMGSVGRSSFGRHDTRDLSTGVESGGVSRPYEFGDTLNMDVSETLFNAVRREGVKVP
                     IEMTYSDLMVHQCEYHSSCATVLLLDCSHSMILYGEDRFTPAKRVAMALSHLIRTQYP
                     GDTLSLVLFHDSAKEVPLGELARVQVGPYYTNTREGLRMAQRILLR"
BASE COUNT          246 a          314 c          295 g          204 t
ORIGIN      
        1 aaccgcagga gcagcgtgaa actccgtcgt cgcgtggacc tcgccgccag ttcaattagt
       61 agaagcgcgc acaaatgtag ctagtcggac cccgtggaaa ttatgaaagt aattcgttac
      121 ggaaaatatg ttggtgagcc ggcggacgcc gtcgaccttg acgagcttgt gaagcgaatg
      181 ggcgactttt ttctgcagag cggctttgag tcccaatatt atggcgtctc ggaaatggac
      241 aaccaacgct ccatggaagc gcttaaagag gctatcttgc gggcgctgca ggaaggcgac
      301 ttgctgcccg aagacgccat gagcgaagag ttacgcaaga tgctgcagag cccccaagcg
      361 cagaatagcc aagcggtgaa agatttgctg gaaaaattaa tggaccggct tgcgcaagag
      421 ggtttcatca atccgcagca gcctccgcaa gtcacgcccc cgcatcagaa tacggcgcgc
      481 ggcagcctcg gtcagccgca agagcgacaa accgaagcgc gcttcgaaat caccgacaag
      541 accatcgact ttctcggttt caaaactttg caggatttga tgggctccgt cgggcgcagc
      601 agctttggcc gccacgacac ccgtgaccta tcgactggcg tcgaatccgg cggcgtctcg
      661 cgcccgtacg aattcggcga cacgttaaat atggacgtga gcgaaacgct ctttaacgcg
      721 gtgcgccgcg aaggcgtcaa agtacccatc gagatgacct actccgatct gatggtccac
      781 cagtgcgaat atcacagctc gtgcgctacc gtgctgctgc tcgattgcag ccatagcatg
      841 attctttacg gcgaagatcg cttcacgccc gccaagcgcg tggccatggc tctctcgcat
      901 ttgattcgca cgcaatatcc gggagacacg ctgagcctgg tccttttcca cgacagcgcc
      961 aaagaagttc ccctcggcga actcgcccgc gtgcaggtcg gcccttatta caccaacacc
     1021 cgcgaaggtc tgcgcatggc ccagcgcatc ctgctgcgc
//
LOCUS       DATCBB010000164         1030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_394486, whole genome shotgun sequence.
ACCESSION   DATCBB010000164 DATCBB010000000
VERSION     DATCBB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394486"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VII37_01865"
     CDS             <1..850
                     /locus_tag="VII37_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEY5068493.1"
                     /translation="RIPQPVTRIVSLAPSLTETVYALGLQDRLVGDTDYCDYPPEAQK
                     KQKVGGAINPSLEEIAHLRPDVVLVTKHLNTLDTVHSLDALGIASYATDPRNVDEIVA
                     SAKRLGDVLGAAEAGAALAEDLQHRLDVLQQKIGALPPRRVLFVVWADPLISIGKDTF
                     IADALRRAGAVSIVDSKQNWPQVNLEEVARLQPEVLVFADSHSEMTPQHLDVFATRPA
                     WRILNAVRDRNFAVISEAVNRPAPRIVTAIEELAQKLHPEAFGERPVSDKLSGSADDA
                     KCLCAR"
     gene            838..>1030
                     /locus_tag="VII37_01870"
     CDS             838..>1030
                     /locus_tag="VII37_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068494.1"
                     /translation="MRPLTIQRLFAQCAILAAVLFVVVIIALKAGAVEVSLFALGRDL
                     IRVIVHHGGEIKSDYGLIVL"
BASE COUNT          213 a          239 c          343 g          235 t
ORIGIN      
        1 gcggattcca caaccggtga cgcgcatcgt ttcgctggca ccgagcttga cggaaacggt
       61 ttacgcgctg ggattgcagg accggttggt gggggatacc gattattgcg attatccgcc
      121 tgaggcgcag aagaaacaga aagttggggg cgcgatcaac ccgagcctgg aagagatcgc
      181 gcatttgagg ccggacgtgg tgctggtgac caaacacctt aatacactgg acacggtgca
      241 ctcgctggat gcgctgggta tcgcctctta cgctactgat ccgcgaaacg tggatgagat
      301 tgtggcttcg gcgaaacggc ttggagatgt gcttggagct gcggaggctg gggctgcgct
      361 ggctgaagat ttgcaacata gactggatgt tttgcagcag aagattggag cgctgccgcc
      421 gcgccgggtg ctatttgtag tgtgggccga cccactcatt tcaattggca aagatacatt
      481 tatcgctgac gcgttgcgac gggctggagc agtttcgatc gtggattcca agcagaactg
      541 gccgcaggta aatttggagg aagtggcacg cttgcagccg gaagttctgg tttttgccga
      601 ttcacattct gaaatgacac cacaacattt ggatgtgttc gcaacgcggc cggcgtggcg
      661 gattctaaat gcggtgcgcg atcgaaactt cgcggtgatt agtgaggcgg tgaaccggcc
      721 ggcgccgcgc atcgtgacgg cgatcgaaga gttggcgcag aaactgcatc cagaggcatt
      781 tggggagcga ccggtttcgg acaaactgag tggcagtgcc gatgatgcga aatgtctatg
      841 cgcccgctaa ccatccagcg actctttgcg cagtgcgcca tactcgcggc ggttttgttt
      901 gtggtggtga tcattgcgtt gaaggccggg gccgtcgagg tttcgttatt tgcgctggga
      961 cgcgatttga tccgggtcat tgtgcaccat ggcggtgaaa taaagagcga ttacggattg
     1021 atcgtgctga
//
LOCUS       DATCBB010000165         1262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_394500, whole genome shotgun sequence.
ACCESSION   DATCBB010000165 DATCBB010000000
VERSION     DATCBB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394500"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1262
                     /gene="murJ"
                     /locus_tag="VII37_01875"
     CDS             <1..>1262
                     /gene="murJ"
                     /locus_tag="VII37_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY5068495.1"
                     /translation="HIADSFILAYRIPNLFRRLVGEGSMSASFIPVFAGYMREKPTKE
                     VWKFANNLFWTLALVLAIITVLGMVFSPTVIHTFIPASAQHLNWDEAVALNRIIFPYL
                     FFIGLAALAMGILNCFHIFGLPAATPVLLNVAIILFSVGAVWHYFKSPAISLAVGVLV
                     GGSLQFLVQVPSLVQKGMHFDFGISFSHPGIRNVARLMIPRFFGIGIGQINFFVDTYF
                     VNSAKMPQGSLTALYLADRVMELVLGGYAIAVATAILPMMSHQAAAKDYQSLKKTLSF
                     AVRIVAFITIPAALGLMILREPIIRVLFQHGQFVAESTRLTARALLCYAVGLPALASV
                     KLIVPAFYSTHDTRTPVAVASISLVLNIVLNIVFLNYFFNYVQNGGPALATAIACFVD
                     FFALFFIFRMRYGKIGGRQILNSFLKIAL"
BASE COUNT          201 a          388 c          336 g          337 t
ORIGIN      
        1 catattgccg actccttcat cctggcctac cgcatcccca atcttttccg tcgtttggtg
       61 ggcgaaggct cgatgagcgc ctcgtttatc ccggttttcg cgggctacat gcgcgaaaaa
      121 ccgaccaagg aagtctggaa gtttgccaac aacctgttct ggactctggc gctggtgctg
      181 gccatcatca ccgtgctggg tatggtcttc tcgcccaccg tcattcacac ttttatccct
      241 gcttctgccc aacatctgaa ttgggacgag gccgtcgctc tcaatcgcat catcttcccg
      301 tacctgtttt tcatcgggct ggcggcgctg gccatgggca tcctgaactg ctttcatatt
      361 ttcggattgc ctgccgcgac tccggtgctg ctgaacgtcg ccatcatttt gttttccgtc
      421 ggagcggtct ggcattattt caaaagtccg gccatctcgc tggccgtcgg cgtgctcgtc
      481 ggaggttcgc tgcaatttct ggtgcaggtg ccgtcgctgg tgcaaaaggg catgcacttc
      541 gatttcggca tctccttttc gcatccgggg attcgcaatg tggcgcggct gatgattccg
      601 cgcttttttg gcatcggcat cgggcagatc aactttttcg tcgacaccta cttcgtgaac
      661 tcggccaaaa tgccgcaggg cagcttgacg gcgctgtatc tcgccgatcg cgtgatggag
      721 ctggttctgg gcggctacgc catcgcggtg gccaccgcca tccttcccat gatgtcgcat
      781 caggccgcgg ccaaggacta ccagtcgctc aagaaaacgc tgtcattcgc cgtgcgcatc
      841 gtggcgttca tcaccattcc tgcggcgctc ggactgatga ttctgcgcga gccgatcatt
      901 cgcgtgcttt tccagcacgg ccagttcgtg gccgaatcca cacggctgac cgcccgggcg
      961 ttgttatgtt atgcagtggg attgccggcg ctggcctccg tgaaactgat cgttccagcg
     1021 ttttattcta cgcacgacac ccggactccc gttgcggtag cctcgatttc cctggtcctc
     1081 aacatcgttc tgaacatcgt cttcctgaat tatttcttca actacgtgca aaacggcggg
     1141 ccggcgctgg ccaccgcgat cgcctgtttc gtggacttct tcgcgctgtt ttttatcttc
     1201 cgcatgcgct atggcaagat cggcgggcgg cagatcctca actcttttct caagatcgcg
     1261 ct
//
LOCUS       DATCBB010000166         1972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_232058, whole genome shotgun sequence.
ACCESSION   DATCBB010000166 DATCBB010000000
VERSION     DATCBB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232058"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VII37_01880"
     CDS             complement(<1..888)
                     /locus_tag="VII37_01880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5068496.1"
                     /translation="MATPSSTNRRTPEHIFNTLFAYQQTYALRAAIDLDIFSAISGGA
                     TDIPSLAKKSGVAERGLQALCNYLVPLGFLTKQGTHYGLTQDSAIFLNKQSPAYMGAI
                     SQFFTNDHIVGNFAKLSEAVKKGGTADAEGHNQAPNDEMWVTFARTMGPTTAPAAAFV
                     AELTGMSQGKTCKVLDIAAGHGMYGITLARKNPNAQVTALDWPNVLAVAKENAQAAGV
                     GERFETISGNAFEANLGSGYDYVLLTNIFHHFDPPTCEKLMKRVHAALKPGGQAITVE
                     FVPNEDRVSPAMPAMFSLVM"
     gene            1057..1785
                     /locus_tag="VII37_01885"
     CDS             1057..1785
                     /locus_tag="VII37_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY5068497.1"
                     /translation="MKREYHRWYSSRLGIDLSVVVYGHWGPPLMGFPTSAGDEWELEN
                     QGMIGALGDFIEAGRIKFFSVNSVNHHGLYNRGAHPFHRSYMQRMFDEYLREEVVPFI
                     WDSCKSSVAISTMGASFGAYHAANTLFKHPDVFKRCFAMSGVYDVRNFMDGMYDDNFY
                     FNNPVDYLANLSDPWLLGQLASCDIHIITGTGPWEKSGASYRLSEILSSKGIRHSLDD
                     WGPQGGHDWPYWKNEMREYVSRLF"
BASE COUNT          410 a          464 c          599 g          499 t
ORIGIN      
        1 catcaccagg ctgaacattg ccggcatcgc ggggctcacc cgatcttcgt tgggcacaaa
       61 ctccacggtg atggcttggc cgcccggttt caacgccgcg tgcacgcgct tcatcaattt
      121 ttcgcaggtg ggcgggtcga aatgatgaaa aatatttgtg agcaaaacgt aatcgtagcc
      181 gctgcccaag tttgcttcaa acgcattgcc gcttatcgtt tcgaatcgtt cgccgactcc
      241 cgcagcttgg gcgttttcct tggctactgc caacacgttt ggccagtcca gcgcggttac
      301 ctgggcgttg ggatttttgc gcgccagggt gatgccatac atgccatggc cagcggcaat
      361 gtccaacacc ttgcaagttt ttccttggga catgccggtc agctcggcga cgaacgccgc
      421 agcaggagca gttgtggggc ccatggtgcg cgcgaaggta acccacattt cgtcattggg
      481 ggcctgattg tggccctccg cgtctgcggt gccgcctttc ttcaccgcct cggatagttt
      541 ggcgaagttg ccgacgatgt ggtcgttggt aaaaaactgc gaaatcgcac ccatgtacgc
      601 gggtgattgt ttgttcagaa aaatcgcgga gtcctgggtg aggccgtagt gcgttccttg
      661 ttttgtcaga aaaccaaggg gcacgaggta attgcacagc gcttgaagac cgcgctctgc
      721 aacgccggat tttttcgcca gtgaagggat gtccgtggcg ccaccggaga tggccgaaaa
      781 aatatccagg tcgatcgcgg ctcgcaaggc gtaagtctgt tggtatgcga ataatgtatt
      841 gaaaatatgt tccggggtac ggcgattggt cgaagatgga gtagccatta ttaaaattgc
      901 tccttttagc ggatggagga cgccaattgg cagcgaagtg tagcaggaat taaaaagcaa
      961 ttcacttcgg gcgaatgggt cggtagttgg tcagattgct tcgggttcag gcggctggca
     1021 taattctgct cccttcgttg cgcatagagg cgattcatga aacgtgagta tcaccgctgg
     1081 tattcttcac gactgggcat tgacctgagc gtggtggtgt acgggcattg gggaccgccg
     1141 ttgatggggt ttcctaccag cgcgggggat gagtgggaac tggaaaacca ggggatgatc
     1201 ggggcgctgg gcgattttat tgaggcgggc agaattaagt ttttttctgt gaactctgtt
     1261 aatcatcacg ggctttacaa ccggggtgcg cacccgtttc atcgcagcta catgcaaagg
     1321 atgtttgacg aatatctgcg tgaggaagtt gttcctttta tctgggacag ttgcaaatct
     1381 tcggtggcga tttccacgat gggggcttct ttcggggctt atcacgcggc gaatactttg
     1441 ttcaagcatc cggacgtgtt caagcggtgt tttgcgatgt ctggcgtgta cgatgttcgg
     1501 aattttatgg atggcatgta cgacgacaat ttttacttta acaatcccgt ggattatctg
     1561 gcgaatttga gtgatccgtg gctgctgggg cagctggctt cgtgcgacat tcacatcatt
     1621 actgggaccg ggccgtggga aaagagcggc gcgagctacc gtttgtcgga aattttatcg
     1681 agcaaaggta ttcgccactc gctcgatgat tgggggccgc agggcgggca cgattggcca
     1741 tactggaaga acgagatgcg cgaatacgtt tcgcggcttt tctagcgcgt gaatcggcgc
     1801 gacctaacga ttgagaagag ccgcgaagtg gtgcatatgc gtctaaatca gcgaaggctc
     1861 aaccagcggt gttttgaatt tttcccaaac gcgcacacgt gcgcgctctt atttaaagag
     1921 cgaagtgcct gggaatctgg cggctccgcc tagttcttct tcgatgcgca ag
//
LOCUS       DATCBB010000167         9194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_167504, whole genome shotgun sequence.
ACCESSION   DATCBB010000167 DATCBB010000000
VERSION     DATCBB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167504"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VII37_01890"
     CDS             <1..362
                     /locus_tag="VII37_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5068498.1"
                     /translation="NESVIALDLLIDSAEIHAPDRVCREIFQIYREALNNIKKHAKAS
                     HVVVKLSQDDSALVLVVDDNGEGFSFAGRFTGDELDRLRLGPISIKERARTVGGILTV
                     ESNPGHGARLIIEIPLG"
     gene            364..1029
                     /locus_tag="VII37_01895"
     CDS             364..1029
                     /locus_tag="VII37_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5068499.1"
                     /translation="MAKTKQQIRVLLADDHAIFRDGLRKLLDSDEDMSIVGEAQNGVE
                     CIKLLAKLKPDILLLDLRMPDKDGLAVLEEVNFDTLPTRVIVLTAAEDDRDVVRAMRL
                     GARGVVLKQSATDLLVKSIQRVFAGEIWLDNRMTAEVMKAFSKSSESGPRREKPLLSD
                     REKEIVQLVAQGFRNKEIGEKLFISEQTVKNHLHNIFDKLGVSDRLELALYAIHHRLI
                     DQT"
     gene            1172..1507
                     /locus_tag="VII37_01900"
     CDS             1172..1507
                     /locus_tag="VII37_01900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5068500.1"
                     /translation="MTGSERRTTQRFSMRLPLTVRWTTGAAVGETSTESRDVSSRGVY
                     FFLQKDVKEGSAVEILLTLPNEITMAGPVRVRCLGRVQRTEPREEGAVGVVAAIERYE
                     FLRGEEERA"
     gene            1890..4460
                     /locus_tag="VII37_01905"
     CDS             1890..4460
                     /locus_tag="VII37_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY5068501.1"
                     /translation="MGRKMSIANEPATKTETLREIQELDKTETSQSEIDKLEMLAALG
                     EPAKLVAVEVSVAADRVPAANQSAEAQSPDVHLSDVQSSEVPAAHAPDLTTEVAATSN
                     IPLIELAQILDEHKMWLESGGDAGAKADLTAANLAGADLTGVNLQDAILTKANLAGAD
                     LSMANLRGTNLVHADLRNATLLGTELRGANLMGATLYGAEGLWVGRLGGTNLFDALLP
                     ESVAEFSSTNPIAEASKVARFFYFTTLAMSAVCCLMILFTTDVRLLLNTSALPLASLG
                     NALPMTGFYLGAPLVLFALYLRFHFLLLRLWGSIAALPAVFPDGQTPERDGPWFLMGL
                     IRKHFRWFRDGRSPLAVLETAISMALGYWVVPATLLLFWVRYLVRQDFRGTLLQVLLI
                     TVSVATATGLPDIVSRVLRSGEIRITKSKTFFRLTLLTMRAALIIGGVLLMISLGVIR
                     GLPADRDIASDHSSASMRRWASELFQCVGYRPYADLTEAVISTPPAANNWTEAGMAEV
                     PGARLNQMNLRFARAYRAFLMNAKFWRADLEGAYLSEADLRGANMREAILPSANFNRA
                     QVNRGILVSANAVGAQFVSTDLRGADLSYGNFDNADLSMAKLAGSSLYAISLRGARLL
                     RTDLSRTDLRDTKLDHAVLSFANLQDADMSSAKLGGASLSGAQMKNTILLDADLKNAD
                     LRGAFLSGALLRGAEIDGANLAGTEMHGATGLSAGQICSAHWQDALLDAELLSETQAR
                     CGTSGSSANPSRPAPQVNAAPAGTSPVAAASAADVSKLGSAQQGAPSQGDAAAQTSLN
                     PVVEKSVAIKAKHSDTVKAKEASAAQTGSSAKTKQKSSAPENPKTKAGTTAATASVPQ
                     "
     gene            complement(4509..6668)
                     /gene="acs"
                     /locus_tag="VII37_01910"
     CDS             complement(4509..6668)
                     /gene="acs"
                     /locus_tag="VII37_01910"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5068502.1"
                     /translation="MGVRNVAKARVKPQDKQKHSKKNENHDERKLTEAQIAVHWKEEE
                     YFQPSAKFIGQANLNDPSMVEKFSEKHFPECFREYAELLHWDQYWHSTLDTSEPPFWK
                     WFVGGKLNACYNCVDRHLEKYRNKAALIFVPEPEQESATVITYQELYRRVNEVAAMLR
                     DFAKLKTGDRVTIHMPMIPELPITMLACARLGIVHSVVFGGFSGEACGLRAADSGSRV
                     LIYADGYFRNGKWVDHKGNADIAVQTAEKEGQHIDKVLVWRRYSGKYNSESPMIMKRD
                     YFVDEVLRDFRGHIVEPVSMDAEAPLFLMYTSGTTGKPKGCQHRTGGYLAYVTGTSKY
                     YQDIQPNDVYWCMADIGWITGHSYIVYGPMALAATSVLYEGVPQFPDAGRPWRIAERL
                     DVNIFHTSPTAIRMLRKAGGDEPKKYNYHFKHMTTVGEPIEPEVWKWYHQVVGKGEAV
                     IVDTWWQTETGGFLCTTKPALDAMKPGSAGPAALGIYPVIWDENGNEVTAGSGNAGNI
                     CIRNPWPGIMQTIWGDPQRFVDQYYKKYNKHPHSKDWRDWPYYAADGAVLPADGYYRI
                     LGRVDDVINVAGHRLGTKELESACLTISEIAEAAVVPVVDEVKGRVPEVYISLKPGIP
                     PSKAVQDKVMFTIETMIGKIARPKKIHMVPDMPKTRSGKIMRRVLAAISNNLDTGDIT
                     TLANPDVVAQIRELVQGRGVGPLKDGPEDIKRFGEEA"
     gene            complement(6813..8300)
                     /locus_tag="VII37_01915"
     CDS             complement(6813..8300)
                     /locus_tag="VII37_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5068503.1"
                     /translation="MASRTNVVTGGVAEYSIWRVILASSVGTMIEWYDFYIFGSLTAV
                     LALKFYPPGNDTFAYIAYLATFAVGFLVRPFGALFFGRIGDIVGRKYAFLVTLSIMGG
                     ATAVIGLLPTYKTAGWLAPIILIIIRVLQGLALGGEYGGAAVYVAEHVPDHKRGFYTS
                     FIQITATLGLFVSLIVILLTQQSMTKEAFQDWGWRIPFLISIILVTISLYIRLKMKES
                     PIFTQIKTAGMTSAQPLKDAFGKWENLKRVLISLFGATAGQGVVWYTGQFYALFYLQT
                     ILKVPAKTANIIIAIALLIAMPFFTLFGALSDRVGRKWLMMAACLLAVLTYIPIYKSM
                     QGAAGNNVVGVSSAKSKVTGATTLTAMTVDPVTGNRVPAKDATNANVPMLVFLVFLQV
                     LYVCMIYGPIAAYLVEAFPARIRYTSLSLPYHIGNGVFGGLLPLIGLWSCAATGNIYA
                     GLYYPMIVASITFVIGSLLLRETHGTKIWSEVGTTPHVAAGDLTP"
     gene            8559..>9194
                     /locus_tag="VII37_01920"
     CDS             8559..>9194
                     /locus_tag="VII37_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5068504.1"
                     /translation="MTIEQAIFLFFAAAVGGAINAVAGGGSFVAFPALLFTGASPVPA
                     NATNTLALWVGTTASGGAYRQKLDLPRRVMIPLIVTSVIGGVAGALLLIKTPAQTFLK
                     VLPWLMLGATLLFAFGKRLTGSISTGISGETSRRSMVGATIFELAVALYGGYFGGGIG
                     ILNLAMLSVLGMTDIHAMNKLKVILGSIINGVAAVAFVVTGFILWPQAGVMT"
BASE COUNT         2032 a         2320 c         2696 g         2146 t
ORIGIN      
        1 gcaacgagtc ggtcatagcc ctggatttgc tgatcgattc tgcggaaatt cacgctccag
       61 accgtgtttg ccgggagatt ttccagatat atcgtgaagc actcaacaat ataaaaaagc
      121 atgctaaggc ttcccatgtc gtggtaaaac tgtcacagga tgacagcgcc ctggtgttag
      181 tcgtcgacga caatggcgaa ggcttcagct tcgcaggacg attcaccgga gacgaactgg
      241 accgcctgcg gttgggaccc atctccatca aggagcgtgc gcgtactgtc gggggaatac
      301 tgacggtgga atcgaaccct ggacatggag cgcggctaat catcgaaatt ccgctcggtt
      361 gacatggcaa aaactaaaca acaaatacga gtcctccttg cggatgacca cgcgattttt
      421 cgcgacggcc tccgcaaact gctggattcc gatgaggata tgtccatcgt cggagaagcg
      481 cagaacggcg tcgagtgcat caagctgctc gcaaaattaa agccggacat attactactg
      541 gacttgcgca tgccggataa agacggttta gcggtgctcg aagaagtgaa cttcgacacg
      601 ctcccgacgc gcgttatcgt tttgaccgct gcggaagacg atcgcgacgt agtgcgtgcg
      661 atgcgactgg gcgctcgcgg tgtcgttttg aaacaatccg ctacggatct gctggtgaaa
      721 agcattcaac gcgtttttgc gggagaaatc tggcttgata accgcatgac cgctgaggtg
      781 atgaaggcgt tctccaagtc ttcggagtcc gggcctcggc gagagaagcc gctactgagc
      841 gaccgtgaaa aagagattgt gcagttggtg gcacaggggt ttcgtaataa agagattggc
      901 gagaagctgt tcatcagcga gcaaacggtg aagaaccacc tgcacaatat cttcgacaag
      961 ctgggcgtgt cggatcgcct ggaattggct ttgtacgcca tacatcaccg gctaatcgac
     1021 caaacttaag tgtaatagcg gtgtttcgcg cttcgggttg acccgtccta gtacattggt
     1081 acccacaaag ttgttaattc ttttgtaccc tgagtcctat tgttcccaat gtgatacccg
     1141 gatacactgc atttgatcag gaggttccgc tttgaccggc tcagaacgcc gcactaccca
     1201 gcgcttttcc atgcgccttc cgctcaccgt tcgttggacc acaggtgcgg ccgttggtga
     1261 aacaagcaca gaatcccgcg acgtcagctc ccgtggggtg tatttcttcc ttcaaaaaga
     1321 tgtaaaagag ggttctgctg tagaaatact cctgacgctt ccaaacgaaa ttacaatggc
     1381 aggtccggtg cgcgtgcggt gtctcgggcg cgtgcaacgt accgaacctc gtgaagaggg
     1441 cgccgtaggc gtagtggctg caatagaacg ctacgaattt ctacgcggtg aagaagaacg
     1501 cgcctaatta ggtgttttaa cattttcccg atgggaatgc ggcaagcaag ctgcgcaatt
     1561 tctcttcggc gtgacgaaaa attcggcgag cagatgttgt cccccaactg ctgcgtcctc
     1621 cagacttgaa gtttttacgc ggtaacaggt ttcttgctcg cacactgcgg aggcgggcag
     1681 gataagccgg ggctccttgg cccctcagcg aagcgccccg gctctatttt tttgtccgat
     1741 gcgtacctct gtttaactcc cacctcgatt tccagcctaa tcaccggcag ttcactgtga
     1801 ggttgttatc tcggcgcccc tgtcctttgg gtcaggcgac atagacggta ttcataagcg
     1861 atgatgaaaa cagtcggaag ggaaaacgaa tggggagaaa gatgtctatt gcgaacgagc
     1921 ctgccacgaa gaccgaaacg cttcgtgaaa ttcaggagct ggataaaacg gaaacgtctc
     1981 agagcgaaat cgataaattg gaaatgttgg ctgcgctagg agagcctgcg aaactggttg
     2041 cagtagaagt ttctgtcgct gcagatcgcg tgccggcggc gaatcaatct gctgaagcgc
     2101 aatctcctga cgtgcatttg tcggatgtgc aatcttcaga agtgcctgct gcgcacgctc
     2161 cggatttaac taccgaggtg gccgcgactt cgaacattcc actgattgaa ttggctcaaa
     2221 ttctggatga acacaaaatg tggttagaat ccggaggtga cgccggtgca aaggccgatt
     2281 taacggcagc gaatctcgca ggcgccgatt tgaccggcgt gaacctgcaa gacgcgattt
     2341 taaccaaggc gaatttagcg ggcgcggatt tgtcgatggc caatctgcga ggaacgaatc
     2401 tagttcacgc cgatttgcgt aacgcgacac tgttaggcac cgaattgcgg ggcgccaacc
     2461 tgatgggcgc aactttatac ggcgccgaag gcctgtgggt tggcaggtta ggcggcacga
     2521 atcttttcga cgcgctattg ccggagtcgg tggcggaatt cagcagcacg aatccgatcg
     2581 cggaagccag taaagtggcg agattctttt atttcacgac tctggcgatg agcgccgttt
     2641 gctgcttgat gattcttttt actacggatg tgcgcctgct tctcaatacg tctgcgttgc
     2701 cgctcgcatc tctaggcaat gcgctgccta tgactggatt ttatctcggg gcaccgctgg
     2761 tgctatttgc gttgtacttg cggtttcatt ttttgctgtt gcggttgtgg gggagtatcg
     2821 ccgcgcttcc agctgttttt ccggatggac aaacgccgga acgagacggt ccatggtttt
     2881 tgatgggatt gatacgtaag cattttcgat ggtttcgtga cgggcgctcg cccctggcgg
     2941 tgctagagac tgcaatttcc atggcgctag ggtattgggt agtgcccgcg acgttgctgc
     3001 ttttctgggt gcgttacctg gtgcgccagg actttcgcgg aacgctgctg caagtcttat
     3061 tgatcacagt ttcagtagcg accgccacgg gcttgccaga cattgtctct cgcgtgttgc
     3121 gatcggggga aatccgcatt acaaaatcaa agactttctt tcgactgacg ctgttgacga
     3181 tgcgcgcggc gctaatcatc ggcggtgttc tgctgatgat ttcgcttggg gtgattcgag
     3241 gattgcctgc cgaccgagac attgcttccg accattcttc ggcgagcatg cggcgctggg
     3301 cctcggaact atttcagtgc gtcggctatc gtccatatgc ggacctgacg gaggcagtta
     3361 tctccacgcc gccagccgcc aacaattgga cggaagcggg aatggcagaa gtgccgggcg
     3421 cgcgacttaa tcagatgaat ctgcgttttg cacgcgctta tcgcgctttt ctgatgaatg
     3481 caaaattttg gcgggccgat ctggaaggcg catatctttc ggaagcggac ctgcgcggcg
     3541 cgaacatgcg tgaagcgata ctgccctcgg ccaatttcaa tcgggcgcaa gttaaccgcg
     3601 ggattctggt gtcggccaat gctgttggcg ctcaattcgt ttccacagac cttcgcggtg
     3661 ccgatctttc ctatggaaat ttcgacaatg cggacttgtc gatggccaag ctggcgggat
     3721 cgtcattgta tgcaattagt ttgcgcggag cgcgattgtt gcgaacagac ctgtcgcgga
     3781 cggacttgcg cgataccaaa ctcgatcatg cggtcctttc gtttgccaat ctacaggatg
     3841 cggatatgtc atcggcaaaa cttggcggag cgagtctgtc gggcgcgcag atgaagaaca
     3901 cgattcttct ggatgcggac ttgaagaatg ccgatttgcg cggagcgttt ctgagcgggg
     3961 cactcttgcg aggcgcggaa attgacggtg caaatcttgc gggaacggag atgcacgggg
     4021 cgaccggact gtcggcgggg caaatatgct cggcgcactg gcaagatgca ttgcttgatg
     4081 cggaattgct cagcgaaact caggctcggt gtggaacgtc cgggtcaagt gcaaatcctt
     4141 ccagaccagc gccgcaggtt aatgccgcac cggctggtac ctcgccggtc gccgctgcgt
     4201 ctgctgcgga cgtatctaaa cttggttcag cgcaacaggg cgcgccctcg caaggagatg
     4261 cggcggcgca gacaagtttg aaccctgtcg tggagaagtc agtcgctatc aaagctaaac
     4321 acagcgacac agtaaaggcc aaagaagcgt cggctgcgca aactggctcc agcgcgaaaa
     4381 caaaacaaaa atcttcagcg cctgaaaatc cgaagacaaa ggccggtaca acggcggcca
     4441 ccgcctccgt accacaataa acgaaggtaa aagccttggt atttaagcgg gacttatgtt
     4501 aacggaaatc aggcttcctc gccgaaacgc ttgatgtcct cgggaccatc tttcagcgga
     4561 ccgactcccc ggccctgcac cagttcacga atttgcgcca cgacgtcagg attggcgagg
     4621 gtggtgatgt cgccggtatc caagttgttg gagatggcag ccaatacacg gcgcatgatt
     4681 ttgccggagc gggtcttggg catatcggga accatgtgga tttttttcgg gcgggcgatt
     4741 ttgccaatca tggtctcgat tgtaaacatg accttgtcct gcaccgcttt gctgggagga
     4801 atgcccggct tcagtgaaat atacacttcg ggtacgcggc ctttcacttc gtcgaccacc
     4861 ggtacaactg cggcttccgc aatttctgaa atagtaaggc acgcggactc cagttccttg
     4921 gtgcctaaac ggtgtccagc aacattgatg acgtcatcga cgcggccaag aatgcggtaa
     4981 tagccgtcag cgggcaacac cgcgccgtcg gctgcatagt atggccagtc ccgccagtct
     5041 ttgctatgcg gatgcttgtt atattttttg tagtattgat caacgaatcg ttgtggatcg
     5101 ccccagatgg tttgcatgat gccgggccaa ggattgcgga tgcaaatgtt tccggcattt
     5161 ccggaaccgg cggtgacttc attgccgttc tcgtcccaaa ttactggata aattcccagc
     5221 gcagcagggc ctgcgctgcc cggcttcatc gcatccaagg cgggtttagt ggtgcacaga
     5281 aaaccgccgg tttcggtttg ccaccaggta tcaacgatga cagcttcgcc tttaccgacg
     5341 acctggtgat accacttcca gacctccggt tcgatgggct cgcccacggt ggtcatgtgc
     5401 ttgaagtggt agttgtattt tttgggttcg tcgccgccag ccttgcggag catgcgaatg
     5461 gcggtaggcg aagtgtgaaa aatgttgacg tcgaggcgct cggcgattcg ccaagggcgg
     5521 ccagcgtcgg ggaattgcgg gacgccttcg tacaaaacgc tcgtggcggc cagcgccatg
     5581 gggccataga cgatgtagga atggccggtg atccatccaa tgtcggccat gcaccagtaa
     5641 acgtcattag gctgaatgtc ctggtaatac tttgaggtgc ccgtcacgta cgcgaggtag
     5701 ccgccggtgc gatgttggca gcccttgggc ttgccggtgg tgccactggt gtacatgagg
     5761 aagaggggag cttcggcatc catggaaacg ggctccacga tgtgtccgcg aaaatccctc
     5821 aaaacttcat cgacaaaata atcacgcttc atgatcatgg gcgattcgga attgtatttt
     5881 ccgctataac gccgccagac tagaactttg tcgatgtgct gcccctcttt ttcggctgtt
     5941 tgcacagcga tgtcggcatt gcctttgtga tcgacccact tgccgtttcg gaagtagcca
     6001 tcggcgtaaa tcaacacgcg actgccggaa tccgcggcgc ggaggccgca ggcttctccg
     6061 ctaaagcctc caaacaccac ggaatgaaca attcctagcc gcgcgcaggc gagcatggtg
     6121 atgggcagct cgggaatcat gggcatgtga atggtgacgc gatcgccagt ttttaatttg
     6181 gcgaaatcgc gcagcatagc ggcgacttca ttcacacggc gatacagttc ctggtaggtg
     6241 attaccgtgg cggattcttg ttctggttcg ggaacaaaga tgagcgcagc tttatttctg
     6301 tatttctcta gatggcgatc tacgcagttg taacaagcgt tgagtttgcc gccgacaaac
     6361 catttccaga aaggtggctc actggtgtca agggtggagt gccaatactg gtcccagtgc
     6421 agcagttcgg cgtattcgcg gaagcattcg ggaaaatgct tctcgctgaa cttctccacc
     6481 atggaaggat cattgagatt ggcctggcct atgaattttg cggagggctg gaagtactct
     6541 tcttccttcc aatgcacggc aatctgagct tcggtcagct tgcgttcgtc gtggttttca
     6601 tttttctttg aatgcttttg tttgtcttga ggttttacgc gagctttggc cacgttacgg
     6661 actcccatga agaagatgta attgtgccgt caccgagcat aacgccactt ggcgttgcgc
     6721 ctcaaggcag agaccggaga tcgcgcgtga ggcaacgctg ttagctgccg gtcatgcgcg
     6781 cctcatcgtt tgaatgacaa gcgcctcgtg aactacggtg tcaaatcacc tgcggcgacg
     6841 tgcggtgtgg tgccgacttc gctccagatt ttcgtgccgt gagtttcacg cagcaacaag
     6901 ctgccgatta cgaaggtaat cgaggcaacg atcatcgggt aatacaagcc ggcatagata
     6961 tttcctgtag cggcgcatga ccataatcca atgagcggca gcaagccgcc gaacacgcca
     7021 ttgccgatgt ggtagggaag cgatagcgac gtatagcgaa tcctggcggg gaaagcctcc
     7081 acaaggtaag ccgcgatggg cccgtagatc atgcagacat aaaggacctg caggaaaaca
     7141 agaaatacca gcatgggaac gttggcgtta gtggcgtctt tcgctgggac gcgattcccg
     7201 gtgacgggat ctaccgtcat ggcggtgagg gtggttgcac cggtaacttt gctctttgca
     7261 gagcttacgc ccaccacatt atttccagcg gctccttgca tacttttata tatagggatg
     7321 taagtaagga cggctagcaa gcaggccgcc atcattaacc atttacggcc gacacgatca
     7381 gagagtgcgc cgaaaagtgt gaagaaaggc atcgcgatca gcaatgcaat cgcgatgatg
     7441 atattcgcgg ttttagcggg aactttcaaa atggtttgta gataaaaaag tgcatagaac
     7501 tggccggtgt accagaccac accttggcca gcggtcgctc caaacaagga aatcagaacg
     7561 cgcttcagat tttcccattt gccgaaggcg tctttcagag gttgcgcgga ggtcatcccg
     7621 gcagtcttga tctgcgtgaa gatgggcgat tccttcatct tcaagcggat gtaaagcgaa
     7681 atggtcacta ggatgatgga aatgaggaag gggatgcgcc agccccaatc ctggaaagct
     7741 tctttggtca tggattgttg agtgagaaga ataacgatca gtgagacgaa tagtccgagg
     7801 gtggcggtga tctgaatgaa gctggtgtag aagccgcgct tatgatcggg tacatgctcg
     7861 gcgacgtaca ccgctgcgcc gccatattcg ccgcccagag ccaggccctg caaaacgcgg
     7921 atgatgatca aaataatggg ggccaaccag ccggccgttt tgtaggtggg caataaaccg
     7981 atcacggccg tggccccacc catgatgctt agcgtgacca ggaaggcgta tttgcggccg
     8041 acgatgtcgc cgatgcgccc aaagaaaagt gcgccgaaag ggcgtaccag aaagcccacc
     8101 gcgaaagttg ccaggtatgc gatgtacgcg aaggtgtcgt tgccgggggg atagaatttc
     8161 agcgccagga cggcggtgag gcttccaaaa atgtagaagt cgtaccactc gatcatggtg
     8221 ccgactgagg aagcgaggat tacccgccag atgctgtatt ccgcaacccc gcctgttacg
     8281 acatttgtcc ggctggccat acttcctccc gttaccgcga cctgaataca ggcccgacgc
     8341 tgacgacaac gctgcgccag ctcaggcttg cgaaccctac tccttacctt cgtaattgcc
     8401 tccgttagcc tcgcaacgct ggagacgagc ctggagttag aaaaggtagg agaatcgcag
     8461 tgttcatata tgggctgagt cgagttgtca agaatgtact ttgtggtgct tgaagagtgc
     8521 ggttaggggg ctaaaggcat tcgtgttaaa acacagtcgt gacaatcgag caggcaattt
     8581 ttctcttttt tgcggcagcg gtcggcggcg caattaatgc agttgcgggc ggcggaagct
     8641 ttgtggcgtt tccggcgctg ctattcacag gcgcatcgcc tgttccagcg aacgctacga
     8701 acaccctggc gttatgggtg ggcaccaccg cgagtggcgg ggcttaccgc cagaagctgg
     8761 atcttccgcg ccgggtcatg attccgttga tcgtgaccag cgtgattggc ggggtcgcgg
     8821 gcgctcttct gctgatcaag acaccggcgc agacgttcct gaaggttttg ccgtggctaa
     8881 tgctgggggc gacgttgctt ttcgcgtttg gcaagcgctt gaccgggagt atatccacag
     8941 gaatctcagg cgagacgagc cggcgctcca tggtgggggc tacaattttc gaactggcgg
     9001 tggcacttta tggcgggtac tttggcggag gtattgggat attgaatctt gccatgcttt
     9061 ctgtgctggg tatgacggat atccacgcga tgaataaatt gaaggtgatt ctggggtcga
     9121 tcattaacgg agtcgcggcc gtcgcttttg ttgttaccgg atttattttg tggccgcagg
     9181 cgggcgtcat gacc
//
LOCUS       DATCBB010000168         1703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_264827, whole genome shotgun sequence.
ACCESSION   DATCBB010000168 DATCBB010000000
VERSION     DATCBB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264827"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VII37_01925"
     CDS             complement(<1..1200)
                     /locus_tag="VII37_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY5068505.1"
                     /translation="MRAPLHHLLIALLAALCLAASAVHAQRVRGELRIEVHDSDGAAV
                     AADAELLSEANQLHRTFKIAQDGHYTAQDLPFGVYRLTINAEGFAPWSKLVDIRSEVP
                     VRIPVTLGVAPVATQIQVTDSATLVDPSRTGSIYSVGRQTIDQEMAAQPGRNLSDLVN
                     QQPGWLYEANGVLHPRGSEYDVQYIFDGLPLTQNRSPAFAPEFDADDVESMRVLTATF
                     PAEYGRKLGGVVEVTTVKDVPAGLHGQLDINGGSFSSISGSAAISYAVGENRLSVSGN
                     GFHTDRYLDPPVLENFTNRGNAAGFSASYEREFSGSDRLRFTIIHNAVRFLVPNELVQ
                     QQAGQRQDITNLETSGQVFFQHTISPNLFLSLSGSVRDASADLTSNPLATPVIVSQSR
                     GYREGYVR"
     gene            1478..>1703
                     /locus_tag="VII37_01930"
     CDS             1478..>1703
                     /locus_tag="VII37_01930"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY5068506.1"
                     /translation="MRRLLCTIGFTGKSAEGFFALLRDAKVQSVIDVRQNRTGQLSAF
                     AKHPDLEFFLRQIGNIGYRHEPLLAPTPELR"
BASE COUNT          368 a          430 c          543 g          362 t
ORIGIN      
        1 gcgcacgtag ccctcgcgat agccccgcga ttgggaaacg atgaccggag tggctagcgg
       61 atttgaagtg agatcggcgc tggcatcgcg cacgcttcct gagaggctga gaaataaatt
      121 gggagaaata gtgtgctgga agaaaacctg accgctggtt tccagattgg tgatgtcctg
      181 acgctggccg gcctgctgtt gaactaactc atttggcacg aggaagcgca ccgcattgtg
      241 aattatggtg aagcgcagac gatcactgcc ggaaaattcg cgctcgtaag aagctgagaa
      301 gccggctgcg tttccgcggt ttgtaaaatt ttcgagcacc gggggatcga gatagcgatc
      361 ggtatgaaac ccgttaccgc taactgacaa gcggttttcg ccaacagcgt aggaaattgc
      421 tgcggagccg ctaatgctgg aaaaacttcc gccgtttatg tccaattgcc catgcagacc
      481 ggcgggaaca tctttcacgg tggttacttc gactacgccg ccgagcttgc ggccatattc
      541 cgcggggaat gtggccgtca gcacgcgcat ggattccacg tcgtccgcat cgaactctgg
      601 ggcgaaggcg ggagagcgat tctgggttag cgggagtcca tcgaaaatat attgcacgtc
      661 gtactcagac ccgcgagggt gaagaacgcc attggcctcg tagagccagc caggttgttg
      721 attgacgaga tcgctgaggt tgcggccagg ctgcgcggcc atttcttgat cgatggtttg
      781 cctgccgacg gagtaaatgg agccggtgcg gctgggatcg acgagcgtgg cggagtcggt
      841 cacttgaatc tgggtggcaa ctggggctac gccgagagta acggggatgc gcactggaac
      901 ctcggagcga atgtcgacaa gtttgctcca aggcgcgaat ccttcggcgt tgatggttag
      961 gcgatagacc ccgaagggca aatcctgcgc ggtgtagtgg ccgtcttgcg cgatcttgaa
     1021 ggtgcgatgt agttggttgg cttcgctgag caactcggcg tcggcggcga ctgctgcgcc
     1081 gtctgaatcg tggacctcga tgcgcaattc tccgcggacg cgctgggcgt ggacggcgct
     1141 cgcggcaaga caaagagctg cgaggagagc aatgagcaga tgatggagtg gagcgcgcat
     1201 cttgatcgtc agtggtaaat acgttttcga gctttctcga aggccgcgag ctgccaatgc
     1261 gattagaact ggatcatcgg gtcgcactgg tgcctggcgc cgcgacggac ttttctctgc
     1321 agcgaaccct cgagacgctt ttttacgtta acacgaagga caccgcggct aagggagccc
     1381 gggggacacg cagcaactaa acagcaacaa ttaaaatacg taacggtttg ctaatatttc
     1441 agctttcggg gctcgcgccg cgaacctgtt gggaattatg cgtcgtctgc tgtgcaccat
     1501 tggctttacc gggaaaagcg ctgagggatt tttcgccttg cttcgcgacg cgaaggtcca
     1561 aagcgtgatc gatgtgcggc agaatcgcac tgggcaactt tccgctttcg ctaaacaccc
     1621 ggacctggag tttttcctgc gtcagatcgg gaacatcgga tacaggcacg aaccgttgct
     1681 ggcgcctacg ccagagctaa gaa
//
LOCUS       DATCBB010000169         1262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_394620, whole genome shotgun sequence.
ACCESSION   DATCBB010000169 DATCBB010000000
VERSION     DATCBB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394620"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="hemC"
                     /locus_tag="VII37_01935"
     CDS             complement(<1..569)
                     /gene="hemC"
                     /locus_tag="VII37_01935"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5068507.1"
                     /translation="MSKTTHPGPARLVIASRESALAMWQAEHIRDRLRALYPQTEVSI
                     LGMTTQGDQILNVTLSKIGGKGLFVKELEAALEDGRADLAVHSLKDVPMNLPDGFVLA
                     AIGEREDPHDAFVSNKFENLAALPAGSVVGTSSLRRESQLRARFPNLKIEPLRGNVQT
                     RLRKLDEGQYAAVILAAAGLKRLGLGSRIR"
     gene            671..>1262
                     /locus_tag="VII37_01940"
     CDS             671..>1262
                     /locus_tag="VII37_01940"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY5068508.1"
                     /translation="MNLIAAPTDISSKDLPLRDDVRLLGRILGDTLREQEGEASYQLI
                     ENVRRAAVRFRKTQDDRDRVQLEQTLDALSPSDTLVVVRAFSYFSQLSNIAEDLHHNR
                     RHRAHLKAGSPPKDGSLTLALERIEEKHISKETLQAFLNSALISPVLTAHPTEVQRKS
                     ILDCQLIISRLLSDRDRVDMTPDDLADNEETLHRFVL"
BASE COUNT          258 a          380 c          354 g          270 t
ORIGIN      
        1 cggatgcggc taccgaggcc cagacgtttc agcccggcgg cggcgaggat gacggcggca
       61 tattgccctt cgtccagttt gcgcaaacgg gtttgcacat taccgcgcag cggctcgatt
      121 tttaaattcg ggaaacgcgc gcgtaactgg ctctcgcggc gcaggctgga ggtaccgacc
      181 acgctgcctg ccggtaatgc cgccagattt tcaaatttat tcgagacaaa agcatcatgc
      241 gggtcttcgc gttcgccaat ggcggccagc acgaaaccat ccggcagatt catcggcacg
      301 tctttcagcg aatgcacggc gaggtcggcg cgaccgtctt ccagcgcggc ttccagttct
      361 ttgacgaaca atcccttgcc tccgatcttg gacagcgtga cattcagaat ctggtcgccc
      421 tgagtggtca tgcccaatat gctgacctcg gtttgcggat ataatgcgcg cagcctgtct
      481 ctaatatgtt ctgcctgcca catcgccagc gcactttcac gcgaagctat cactaatcgg
      541 gccgggcctg ggtgggttgt cttgctcata atattgcgcg ggattttgtg cctgtaatta
      601 ttttaaataa acgaacgatc ttcggcgcat tctaacataa ggtgcgccgt gcttttgtcc
      661 cgagaacgtg atgaatttga ttgccgcccc taccgatatt tccagcaagg atttgccgct
      721 gcgcgatgat gtgcgcctgc ttggccgcat tctcggcgac acgctgcgcg aacaagaggg
      781 cgaagcatcc taccagttga tcgagaacgt gcgccgcgcc gcggtgcgtt tccgcaagac
      841 gcaggacgac cgcgaccgcg tgcaacttga gcaaacgctg gacgcgctgt cgcccagtga
      901 tacgctggtg gtggtgcgcg ctttcagcta cttttcgcag ctgtccaaca tcgccgaaga
      961 cctgcatcac aaccgccgcc accgcgccca cctcaaggcc ggcagcccgc ccaaagacgg
     1021 cagcctgacg ctggcactgg aacgcatcga agaaaagcat atcagcaagg aaactctgca
     1081 agcctttttg aacagcgcgc tgatctcgcc ggtgttgacc gcgcatccca ccgaagtgca
     1141 gcgcaagagc atcctggatt gccagttgat tatttcgcgt ctgctgtccg atcgcgatcg
     1201 cgtggacatg acgccggatg acctggcaga caacgaggag accttgcacc gcttcgtgct
     1261 ga
//
LOCUS       DATCBB010000170         2907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_69721, whole genome shotgun sequence.
ACCESSION   DATCBB010000170 DATCBB010000000
VERSION     DATCBB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69721"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="VII37_01945"
     CDS             <1..2154
                     /locus_tag="VII37_01945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5068509.1"
                     /translation="DLPPGPDLIFEGDGDVWHFIDTPKHGRRSFTYDPSTDLFTGEKF
                     EIYPLSYHIDQLGAAKKKLAITFDDGPDTRWTPKILDVLKAKNVPATFFIVGVAGSQW
                     PELLKREYAEGHEVGNHTYTHPNFDDISNTQLRWELNLTQRLIESTLGVKSILFRPPY
                     GIDHEPEYAEEVAKLPVAQDMGYVIVGQKIDPHDWKSPGGKQIPASDIADNVVTQASE
                     GNIILFHDGGGDRTQTVAALPQIIDRLRAQGYQFVSVPDLLHKTRASMMLPLSPQERF
                     EARADGFIFSLYRWFRVGFATIFIVGIILVSGRAVVIGILALIEKLRPDHAVISDRPP
                     DVTVLIPAHNEENVIVQTVSSVLATNTKNLQIIVVNDGSTDRTGALLDEHFGADSRVH
                     IIHQVNRGKSAALSLAMSQAKTKIVVTIDADTEIEPDAISKLLRHFSDPRVGAVAGNV
                     KVGNRTRWLTRWQALEYITSQNMEKRAFDLLNCITVVPGALGAWRTEAIDAAGGITSD
                     TVAEDADLTIAIRRLGWRISYDEEAIAWTEAPETPGALIRQRFRWTFGTLQSFWKHRD
                     TLLRPKYGTLGWIALPNIFVFQLVLPLISPIIDLMFLGSIALWALGQLHIARLPQLWT
                     TADVQRSLFFFVGFLLIDVLTCMVAFALERKEDWTLLIPVLLQRFYYRQLMYVVLFRS
                     VKEAVSGRPVGWRGVEPDSPRAPRAPEAPPRTVPA"
     gene            2278..>2907
                     /locus_tag="VII37_01950"
     CDS             2278..>2907
                     /locus_tag="VII37_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HEY5068510.1"
                     /translation="MNLFVIGLLASGTLRAQDTPAPKAQTTPAPKSQTTPAAKTPGTT
                     AAKPHTATGARTPLTLKTDKDKVSYAIGVNIGKSMRKDTVDVDPAIFSRGMKDALAGG
                     KLLLTDDEMKVTLTKLQTELRARQEVAMQKAGDINKKAGEDFLAQNKTKEGVVALPDG
                     LQYKILKAGNGPKPAPTDSVVCNYRGTLLDGTEFDSSYKRGQPATFPVSG"
BASE COUNT          678 a          833 c          755 g          641 t
ORIGIN      
        1 gaccttccac cggggcccga cctgattttc gaaggcgatg gtgacgtgtg gcacttcatc
       61 gataccccga aacatggtcg tcgcagtttt acatacgatc cctcaacgga tctgtttacc
      121 ggggaaaagt tcgagattta cccgctttcg tatcacattg atcagctcgg cgcggccaaa
      181 aagaaattgg ccatcacctt tgacgacggc ccggacacgc gctggacccc aaagattctc
      241 gacgtgctga aagcaaaaaa cgtgcccgct acatttttta ttgtcggtgt cgccggaagc
      301 cagtggccgg aattgctcaa gcgtgaatac gccgaaggcc acgaggtggg gaaccatacc
      361 tatacccatc caaactttga cgatatttcg aatactcagt tgcgttggga actgaatttg
      421 acccagcgat taatcgaaag cacgctcggg gtcaaatcca ttttgttccg cccgccttac
      481 ggcatcgatc atgagccgga atacgccgag gaagtcgcca aattaccggt ggcccaggat
      541 atgggttacg tgatcgtcgg acaaaaaatc gatccgcacg attggaagtc gcccggaggc
      601 aaacaaattc cagcttccga cattgccgat aacgtcgtga cgcaggcgag cgaaggcaac
      661 attattcttt ttcatgacgg cggcggtgac cgcacgcaaa cagtagcggc gttgccccaa
      721 atcatcgatc gccttcgcgc gcaaggctac cagttcgtat ctgttccgga tttgctgcac
      781 aaaacgcgcg catcaatgat gctgccgctg tctccgcagg agcgcttcga agcgcgcgcg
      841 gatggattca tcttcagttt gtaccgttgg ttccgcgtgg gattcgccac catcttcatt
      901 gtgggaataa ttttggtaag tggccgagcg gtggtcatcg gtattctggc gttgatagaa
      961 aaacttcggc ccgatcacgc cgtgatttcc gatcggcctc cggatgtcac tgtgttgatc
     1021 ccggcccata acgaagaaaa cgtgatcgta cagaccgttt cttccgtctt ggctaccaac
     1081 acaaaaaatc tgcagatcat agtggtgaat gacggctcta ccgaccgtac cggcgcgcta
     1141 ctcgacgaac acttcggtgc ggactcgcgc gtgcatatca ttcatcaggt aaaccgcggc
     1201 aagtccgccg ccctcagcct ggccatgtcg caagcaaaga cgaaaatcgt ggtaaccata
     1261 gatgccgata cggaaatcga acccgacgcc atctccaaat tgttgcgtca tttctccgac
     1321 ccgcgcgtcg gcgcagtagc cggtaacgta aaggttggca atcgcactcg ctggctcacc
     1381 cgctggcagg ctctggagta catcactagt cagaacatgg agaagcgcgc tttcgacttg
     1441 ctcaactgca tcacagttgt gcccggcgcc ctcggagcgt ggcgcaccga ggccatcgac
     1501 gctgcgggtg gaattacgtc agacaccgtt gcggaagacg cggacctcac tatcgccatt
     1561 cggcgcctag gctggcgcat cagctatgac gaagaagcga ttgcttggac cgaagctcca
     1621 gaaacccctg gagcgctgat tcgccagcga tttcgctgga ctttcggtac gctgcaatct
     1681 ttctggaagc accgcgacac cttgctgcgt cccaaatacg gcacgctggg ctggatcgcg
     1741 ctgccaaata tttttgtatt tcagctggtg cttccactca tttcgccgat catcgatttg
     1801 atgtttctcg gctccatcgc actgtgggcg ctaggccaac tgcatatcgc gcgactgccg
     1861 cagctttgga ccactgccga cgtgcagcga tctttatttt tcttcgttgg atttttgctc
     1921 atcgatgtac tcacctgcat ggtggccttt gcgctggaac gcaaggaaga ttggaccttg
     1981 ctcattcctg tgcttttgca gcgcttttac taccgccagt tgatgtacgt agtacttttc
     2041 cggtccgtga aggaagcggt cagcggccgt cccgtaggtt ggcgtggtgt agagccagat
     2101 tctcctcgag cgccgcgtgc tcctgaagcg ccgccaagaa cagttcccgc atagaattcc
     2161 ttacgatcgc caaactccga atatctattt accacttggg cttttcgtcc cattacattt
     2221 gcacagcttg cagcccaatg tgacaatact gctatgcaaa aaacattaat cttagctttg
     2281 aatttgtttg taatcggttt gcttgcgtcc ggcaccctgc gtgcccagga cactcccgcg
     2341 cctaaagctc aaacaacgcc ggcgcctaaa tctcaaacta ctccggctgc aaaaactccg
     2401 gggactacgg cggccaagcc gcacaccgcg accggcgcga gaaccccgct gacacttaaa
     2461 accgacaaag ataaggtcag ctacgccatc ggcgtaaaca tcggcaagag catgcgcaaa
     2521 gatactgtag acgtggatcc cgctattttc agtcgcggaa tgaaagacgc gctcgccggc
     2581 ggaaaattgc ttttaaccga tgatgaaatg aaggtcactc taaccaagct gcaaacggag
     2641 ctgcgagcca ggcaggaggt agccatgcaa aaagcggggg acatcaacaa aaaggccggc
     2701 gaagacttcc tggcacaaaa caaaaccaag gaaggcgtcg tcgctttgcc ggatggtttg
     2761 caatataaaa tcttaaaggc cggcaacggc ccgaagcccg ccccaaccga ttcggtcgtt
     2821 tgcaactacc gcggcacgct cctagacggc accgaattcg acagttccta taagcggggc
     2881 caaccggcca cgtttcccgt tagtggc
//
LOCUS       DATCBB010000171         2432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_199792, whole genome shotgun sequence.
ACCESSION   DATCBB010000171 DATCBB010000000
VERSION     DATCBB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199792"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VII37_01955"
     CDS             complement(<1..728)
                     /locus_tag="VII37_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY5068511.1"
                     /translation="MSNVSLNKSSQSLTVIPLGGLGEFGMNMMALRFGEDIIVIDAGM
                     MFPESELLGVDLVIPDITYLKQNRAHVRAIVLTHGHEDHIGALPYILRDLNVPVYGTR
                     FTLALVKKRLEEAGLLQSTTLREVLPGRRVEIAPYEVEFISVTHSTIDCVALAIRTPV
                     GVIIHTGDFKIDQTPVGGVPFDLHAFARYGNEGVLALFSDSTNVERPGFTPSERAIVP
                     RIEELCRSAPRRVILSCFASSIHR"
     gene            complement(903..1211)
                     /locus_tag="VII37_01960"
     CDS             complement(903..1211)
                     /locus_tag="VII37_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY5068512.1"
                     /translation="MIATMLATFLESRVVVVEYQRSILGWIIIGLIAGWLAGKIARGE
                     GYGCITDIVLGLVGSLLGGWIFTRLGILGGGFIYSLAAATLGAFILVSIVHLIAGGPR
                     "
     gene            complement(1334..>2432)
                     /locus_tag="VII37_01965"
     CDS             complement(1334..>2432)
                     /locus_tag="VII37_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY5068513.1"
                     /translation="RGIMPQRRTNNSIPFDLAYYMPAEWEPHEATWLGWPHEVTDWPG
                     KFATIPWAFAEIVRLLSLVERVFLLVENRAAELRVRSILKKAGANLGAVNFLRVPTDR
                     GWMRDSGPICVRNSNGDVAYNHFSFNGWAKYPNHKKDAMGVEKANRQLKHRLWQPIHK
                     GRRVVLEGGSIDVNGRGTLLTTEECLLSKTQERNPGFTREDYAEIFREYLGVKKVLWL
                     NRGIAGDDTHGHVDDLARFVNPTTVVTVVEQDKTDENYAPLQENLSRLKEMVDQDGTT
                     LRVETLPMPRPVYFDGQRLPANYANFYIVNGLVLVPTFNDANDRVALHKLAELFPDRK
                     VVGVACRDLVLGLGTIHCMTQQQPAKNLEHH"
BASE COUNT          561 a          709 c          614 g          548 t
ORIGIN      
        1 atccgatgaa tcgaggacgc gaaacacgac agaatcaccc ggcgcggagc tgagcggcaa
       61 agctcttcaa tgcgcggcac gatggcgcgc tccgacggcg tgaaccctgg ccgctccacg
      121 tttgtggagt cgctgaagag cgccagtacg ccttcgtttc catagcgggc aaacgcgtga
      181 agatcaaatg gcacgccgcc cacgggcgtc tgatctattt tgaagtcgcc agtatggatg
      241 atgactccga ccggtgtacg tatcgcgagg gctacgcaat cgatggtgct gtgcgttacg
      301 gaaataaatt cgacttcgta tggcgcgatt tctacccttc gccccggcaa aacctcccgt
      361 agcgtcgtcg attgcaacag gcccgcttct tccaggcgct tttttaccag cgcaagtgta
      421 aatcgcgtgc catacacggg cacgttcagg tcgcgcaaaa tatatggcag cgcgccgatg
      481 tgatcctcgt ggccgtgtgt cagcacaata gcgcggacat gtgcgcggtt ctgctttaaa
      541 tacgtaatgt ccggaatgac caggtccacg cccagcagtt ccgactccgg aaacatcatg
      601 cccgcgtcga tgacgataat gtcctcgcca aaccgcaggg ccatcatgtt cattccgaat
      661 tcgcccaaac cgccgagtgg aattaccgtg agggattgcg aacttttatt caatgaaacg
      721 ttactcaagt catctccaag acgcgttgct gaatcgtatc atagagcagt gacgagcaat
      781 ggacgcgcgc gacgcataaa acgcttggtg aggcgaatga aaacacaagt gcgacgtcga
      841 gtttaggtac ttcctgtggt acggcgcatt ttacggacag ccgaactagc gtttagcgtc
      901 gatcaccgag gaccaccggc aatcagatgc acaatggaaa cgagaatgaa tgcacccaga
      961 gttgccgcag cgagcgaata aataaatccg ccgcccaaga tacctaagcg cgtaaatatc
     1021 cagccgccca gtagcgatcc caccagtccc agcacgatgt cagtgatgca gccatatcct
     1081 tcgccgcggg caatctttcc cgccagccat ccggcgatca agccaatgat gatccagccc
     1141 agtatgctgc gttggtattc caccaccacc acccgcgatt ccaaaaacgt cgccaacatt
     1201 gtcgcaatca tggtgcctcc gcaaaccctg tctctctggt cgcaataatg gcggctagtc
     1261 tatcaaaagt cagacgcggc cctataactt ttcgtcaaat tccggcaaac atcatcgagc
     1321 ggttcgaatc gtctcagtga tgttctaaat ttttggcagg ctgctgctgc gtcatgcaat
     1381 gtatcgtccc aagccccagc acaagatcgc ggcaagcaac gcccacaact tttctgtcag
     1441 ggaagagttc ggcgagttta tgtagcgcca cgcgatcgtt tgcgtcatta aacgtcggaa
     1501 ccagcaccag cccgttcacg atataaaaat ttgcgtagtt tgccggaagg cgctggccat
     1561 cgaaatagac cggtctgggc attggcaacg tttccacacg cagcgttgtc ccgtcctgat
     1621 ccaccatttc ttttagacgt gacagatttt cctgcaaagg cgcgtagttc tcgtcggtct
     1681 tgtcttgttc cacgacggtt acgactgtcg ttgggttcac gaaacgcgcc aggtcatcca
     1741 catgcccgtg tgtgtcatcg ccggcaatgc cacgatttag ccaaagtacc tttttaaccc
     1801 ccagatattc acggaatatt tccgcgtaat cttcgcgcgt gaatcccggg ttgcgctcct
     1861 gcgttttgct gagcaggcat tcttccgtgg ttagtagcgt tccgcgcccg tttacgtcga
     1921 tacttccgcc ttccaatacc acccgcctgc ccttatgaat cggttgccag agccggtgtt
     1981 tcagttggcg atttgccttt tcgaccccca ttgcatcctt tttgtgattc ggatactttg
     2041 cccagccgtt aaatgaaaag tgattgtaag ccacgtcgcc gttggagttt cgaacgcaga
     2101 tcggcccgga atcgcgcatc cacccgcgat cggtgggtac gcgcaaaaaa tttacggcgc
     2161 caagattggc ccccgccttt ttcaggatcg atcgaacgcg caattcggct gcgcgatttt
     2221 caaccaacaa aaaaacgcgc tccaccaatg acagcagacg cacgatttcc gcaaaggccc
     2281 acggaattgt cgcgaatttt cccggccagt ccgtcacttc atgcggccac ccgagccaag
     2341 tcgcttcgtg cggctcccac tccgcaggca tgtaatacgc aagatcaaaa ggaattgaat
     2401 tgttggtgcg gcgctggggc attattccgc ga
//
LOCUS       DATCBB010000172         1074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_102906, whole genome shotgun sequence.
ACCESSION   DATCBB010000172 DATCBB010000000
VERSION     DATCBB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102906"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VII37_01970"
     CDS             complement(<1..288)
                     /locus_tag="VII37_01970"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HEY5068514.1"
                     /translation="MQNLRDRPIRQKITYVIMLISGVVLLVAFAALFFVQAYALKQQS
                     AHELAVVGEITAHNCAAAVMFKDEGAAAEILAGLKTMPQIVSARLELMDQQR"
     gene            572..>1074
                     /locus_tag="VII37_01975"
     CDS             572..>1074
                     /locus_tag="VII37_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068515.1"
                     /translation="MNFYTDEIEEKSAKLGPEMRNQFAGTASSLDSSRKGKRVLTTAV
                     LMAVALAMGFGIWSLSRQLAPELISVSKSPEAAAMAVIPEKGIAVLPFENLSEDQQNA
                     FLADGIQDDIRTALAKVADLKVIDRTSVNTYAAGTRRNLREIAQTLGVAYVLEGSVRQ
                     TGEKVSIT"
BASE COUNT          250 a          293 c          313 g          218 t
ORIGIN      
        1 ccgctgctgg tccatgagct caaggcgtgc actgacgatt tgcggcattg tcttcaatcc
       61 cgccaggatt tcggcggcgg cgccctcatc cttaaacata accgctgcgg cacaattgtg
      121 ggccgtgatt tcacccacga ccgcgagctc atgagcggac tgctgtttga gggcgtaggc
      181 ctggacgaaa aacagcgctg caaatgcgac gagcagcacc acgcccgaaa ttagcatgat
      241 cacgtaggtg attttttgtc ggatagggcg atcgcgcaga ttttgcatgg cactgtcagt
      301 tcacgactga ggattaggga gtggtcgatg catgatttga gccaagcgtg gcggaccgca
      361 gcggaattcg cctcccggct tgcccgcaac gccccagctt cgaaaggaaa gcaacaatcg
      421 tgctacttcc cgttacccga tgagatgggg cgactctgga acacattctg ctcccaaggg
      481 cgggaccgac ggcccgcgca tcccggcacc tgcagtctca ctcacacgaa cctggagaat
      541 aaaaggtcag caggaggttg aggactgttt aatgaatttc tacacagacg agatcgagga
      601 aaagtccgca aagctcggac cggaaatgcg gaaccaattt gcagggacgg ccagttcttt
      661 ggattccagt cgcaagggta agcgcgtttt gactaccgcc gtcctgatgg cggtggcatt
      721 agcaatgggt ttcgggattt ggagtttgtc gcgccagcta gcgccggagc taatcagtgt
      781 gtcaaaatcg ccagaggccg ccgccatggc cgtcatcccg gaaaaaggta tcgcggtgct
      841 cccgtttgag aatctaagcg aagatcaaca gaacgcgttt ttggctgatg gcatccagga
      901 tgacattcgc accgcccttg ccaaagtggc ggacctcaag gttatcgacc gcacttccgt
      961 taatacttac gcggcaggaa cgcgacgcaa cttgcgcgag attgcccaga ccttaggcgt
     1021 tgcctacgtc ctggaaggga gtgtccgcca aaccggtgaa aaagtgagca tcac
//
LOCUS       DATCBB010000173         1194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_265087, whole genome shotgun sequence.
ACCESSION   DATCBB010000173 DATCBB010000000
VERSION     DATCBB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265087"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /gene="uraH"
                     /locus_tag="VII37_01980"
     CDS             complement(<1..301)
                     /gene="uraH"
                     /locus_tag="VII37_01980"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY5068516.1"
                     /translation="MASRMMQVSTHVLDLVQGRPAQDVAVRLEQREADTNWRLLASAR
                     TDQNGRCAQLMPENEVLTPGIYRLVFDTESYFGSRKVETLYPSVEITFYVREGEQH"
     gene            complement(286..789)
                     /gene="uraD"
                     /locus_tag="VII37_01985"
     CDS             complement(286..789)
                     /gene="uraD"
                     /locus_tag="VII37_01985"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY5068517.1"
                     /translation="MVKVLARWNFLSSAAAVNEILPCCGSRAWAAAMVARRPLPEESA
                     LLAASDEIWRSLAEADWLEAFRSHPRIGESRPGESQSVAWSTQEQRGVVDADDGARVA
                     LADGNRTYERRFNRIFIVCATGKSPSEILAILRRRMTNDALTELQEAAEQQRQITQIR
                     LRKWLHG"
     gene            complement(789..>1194)
                     /locus_tag="VII37_01990"
     CDS             complement(789..>1194)
                     /locus_tag="VII37_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068518.1"
                     /translation="ELIRAICLRRAFSGYLHYTRKAGARNAQAISKVCIAAVGRLRGG
                     MTGSAIGGSVIDDVRIALGSVAPVPLRLTEVERVVAGKPVDPELVKAAKAATMAAIQP
                     IDDIRSSARYRTAVAGNLVAEFFNQLRGSENR"
BASE COUNT          269 a          388 c          283 g          254 t
ORIGIN      
        1 aatgctgctc tccctcgcgc acataaaacg taatctccac tgagggatac agtgtttcga
       61 ctttccgcga gccgaaataa ctttctgtgt caaacaccaa gcgataaatt ccgggagtaa
      121 gaacttcgtt ctcgggcatc aactgagcgc atcgcccgtt ctgatcggta cgcgcggacg
      181 ccagcagtcg ccaattggta tcggcctcgc gctgctccaa tcgcacagca acatcctggg
      241 caggacggcc ttgaaccaga tcgagaacat gagtcgagac ttgcatcatc cgtgaagcca
      301 ttttctcagg cgaatctgcg taatctgtct ttgttgctcg gccgcttcct gtaattcggt
      361 tagcgcgtca tttgtcattc ttcgccgcaa gatcgccagt atttccgacg gcgacttgcc
      421 cgtcgcgcac acgataaaaa ttcggttgaa gcggcgttca tacgtccgat tgccgtcagc
      481 cagcgcaact ctagcaccat cgtccgcatc cacgactcca cgctgctctt gtgttgacca
      541 cgcaactgac tgagattctc caggtcgcga ttcaccaatt cgaggatggc tgcgaaaagc
      601 ctccagccag tccgcttccg ccaaactacg ccagatttcg tccgacgcag cgagcagtgc
      661 gctttcttca ggcaacggcc ttcgtgccac catcgccgca gcccaagctc tcgaaccaca
      721 gcacggcagt atctcattca ccgctgccgc ggaggaaaga aaattccatc tggcaagaac
      781 cttaaccatt accggttctc actgccgcgc aattgattga aaaattccgc aaccagattc
      841 ccagccactg ccgtacggta tctcgcgctg gagcgtatat cgtcaatcgg ctggatcgcg
      901 gccatggtcg ccgcttttgc tgccttcaca agctctggat cgaccggctt tccggcaacc
      961 acccgctcca cctccgtcaa tcggagggga actggcgcca cgctacccag cgcaatgcga
     1021 acatcgtcaa tcacacttcc accaatcgca ctcccagtca tgccgccgcg aagccggcct
     1081 acagcggcaa tacaaacttt ggaaattgcc tgcgcgttgc gagcccccgc tttgcgcgtg
     1141 tagtgaagat aaccagaaaa cgcccgccgc aagcagatcg cgcgaatcag ttca
//
LOCUS       DATCBB010000174         1859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_329802, whole genome shotgun sequence.
ACCESSION   DATCBB010000174 DATCBB010000000
VERSION     DATCBB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329802"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VII37_01995"
     CDS             complement(<1..469)
                     /locus_tag="VII37_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5068519.1"
                     /translation="MTEKRLELVRGLGAWASAAIVIGTMIGTGIFLKPAEMAREGRTV
                     SVVFAAWIVGAVLSIFGALSFAELGAAIPEAGGEYAYLRRAFGPVYGFLFGWMHSIVG
                     RPSSLASISAGMMRFVGFLIPAVATPIFTVHAPALGTWIAPYDFTFTWAQPLAV"
     gene            complement(509..>1859)
                     /locus_tag="VII37_02000"
     CDS             complement(509..>1859)
                     /locus_tag="VII37_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY5068520.1"
                     /translation="ALAFSIVATETSTLTIIGTPAIAYGGNLTFLQLIFGYLIGRVLI
                     VFVLLPGYFRGDFFTAYALIEKRFGQRMRAVAASTFLITRALAEGVRVSAIAIVLSVV
                     LGTTEHLSVVIVIALTLLYTLEGGMKAVIWTDVAQLFIYLAGSAATFFVLLHRVPGGW
                     NEVVQVAATHGHKLQILHFSFNLATKYTFWSGLIGGAFLTMATHGTDQTIVQRLLAAR
                     NQRDSRRALLASGVIVLFQFSVFLLIGVLLFVFAQHTPLLSSGERTDRILPLFLLREM
                     PTGLAGLLLASIIAVAMSNASGSLNSLAASSVLDFSALGLSHRRGREPANDAGFLRLS
                     RRMTLFWGLVLMGFGLMTWGPVLEAGLTVASLPFGSLLGLFLLGTLNRRSNAPGALAG
                     MFAGLITILCVWRLTSVAFTWYVLIGSITTFLVGSLVSLLTQNDKTANQRAAVDAAQ"
BASE COUNT          520 a          537 c          510 g          292 t
ORIGIN      
        1 acactgcgag cggctgcgcc caggtaaaag taaaatcata aggcgcgatc catgtaccga
       61 gtgcgggggc gtgcacggta aaaataggtg tggccactgc gggaatgaga aagccgacga
      121 agcgcatcat gcccgccgag atcgaagcca gtgaactggg cctgcccacg atggagtgca
      181 tccagccaaa aagaaatcca tacactgggc cgaatgcgcg acgcaggtac gcgtattcgc
      241 cgcctgcttc cggaatggcc gcgcccagct cagcaaaaga cagcgcgccg aaaatagata
      301 ggaccgcgcc gacgatccac gcggcgaaaa ccacggaaac ggtgcggcct tcgcgagcca
      361 tttcggcagg ctttaaaaag atgccggtac cgatcatggt accaatgacg atggcagccg
      421 aagcccacgc gccaaggccg cgaaccagtt cgaggcgttt ctcagtcatt gccatttgtc
      481 atagttgtga acagtccgat cacaaaattc attgagcagc atccacggcg gcgcgttgat
      541 tcgccgtttt gtcattctgc gtcaagagac tgaccagcga gccaaccaaa aaagtcgtaa
      601 tagaaccgat caacacatac cacgtgaacg cgacgcttgt caggcgccat acgcagagaa
      661 tggtgatgag tcccgcaaac ataccggcca gcgctccagg agcattcgaa cggcgattga
      721 gtgtgcccag aagaaaaagc cccagcaagc ttccgaaagg cagtgacgca acggtcagtc
      781 ccgcttcaag caccggcccc caggtcatca ggccgaagcc catcagcaca agtccccaga
      841 acagcgtcat gcgccgcgac agccgcagaa agccggcatc atttgcgggt tcacgtccgc
      901 gtctgtggct aaggcccagc gcggaaaaat cgagcacact gctggccgcc agagagttca
      961 gcgaaccgct ggcgttggac atggccaccg caatgatgga agcaagcaaa agtcccgcaa
     1021 ggcccgttgg catttcgcga agaagaaaaa gtggcagtat gcggtccgtg cgttcgccag
     1081 acgaaagtaa aggtgtgtgc tgcgcgaaaa cgaagagaag caccccgatc aacaggaaaa
     1141 ctgagaactg aaacagcacg atgacgccac tggccaaaag cgcgcggcgg ctatcgcgtt
     1201 gattgcgcgc cgcgagcagg cgctgaacga tggtttggtc cgtgccatga gtagccatgg
     1261 taagaaatgc gccgccaatc agtccggacc agaatgtata cttcgtcgct aaattaaacg
     1321 aaaaatgcaa aatctgcagt ttatgtccgt gcgtagcggc cacctgcact acttcgttcc
     1381 aacctccggg aacgcgatgc aacaacacaa aaaacgtggc agcgctgccg gcaagataaa
     1441 taaacagttg cgccacgtca gtccagatca ccgctttcat cccgccttcc aatgtataga
     1501 gcagcgttag cgcaatgaca atcacaacag agagatgctc ggtggtacca agcaccacgc
     1561 tcagtacaat cgcgatggcc gagacgcgca caccctcggc cagcgcccgc gtgatcaaaa
     1621 acgtgctggc cgccaccgcg cgcatgcgtt gaccaaaacg cttttcgata agcgcgtaag
     1681 cggtgaagaa atccccacga aaatatcccg gcaaaagcac gaagacaatc agcacgcgcc
     1741 cgatcaaata gccgaaaata agctgcagaa aagtcagatt gccgccataa gcgatagctg
     1801 gagtcccgat gatcgtaagc gtggaagttt ccgtagccac gatggaaaac gccagcgcc
//
LOCUS       DATCBB010000175         1227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_329815, whole genome shotgun sequence.
ACCESSION   DATCBB010000175 DATCBB010000000
VERSION     DATCBB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329815"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VII37_02005"
     CDS             complement(<1..1142)
                     /locus_tag="VII37_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068521.1"
                     /translation="MDNAARLAPGNQDLRIAAGIARLQDQVENHSNPAQTLKWLRAET
                     VPPQQAVDIRLHLAEILSRANLFSEAVIDFAAASALEPDRGDLLYDLALAYFRTGGLD
                     DALASAERAKSVADSGSIESLMGDIQEKRGDPLAAVHSYQAAVTLEPSEERYHLALGL
                     ELLRHQTFDAALVVFKQASALFPQSVRVKILLGLTYFFVDRSAEASQALQDAAKIDPR
                     NETVARYMGEIILQDTSTPAPEAIAQVCTFADEHPQSKTADAFCGGILLKVARDGGDI
                     SRRPEILRRLRHAAHIAPTEAIARCQLGKAFEWSEMWSQARPETETCVRLDPESPDGH
                     YQLSRIYRRLGLTALADQQTALQQGAALRQSEASIRRTETVTKFLVL"
BASE COUNT          273 a          326 c          342 g          286 t
ORIGIN      
        1 agcacgagga attttgttac ggtttctgtg cgacggatgc ttgcttcact ttggcgcagc
       61 gcggcacctt gttgcagggc ggtctgctgg tcggctagag cagttaagcc caaacgacgg
      121 taaatgcgcg atagttgata atgaccatca ggagactcgg gatctaatcg gacacacgtt
      181 tccgtttcag gtctggcctg actccacatt tcggaccatt cgaaagcttt acctaactga
      241 catcgcgcga tcgcttccgt gggcgcaatg tgagcagcgt gccgcagacg acgaagaatc
      301 tcggggcgac gtgaaatgtc cccgccgtca cgggcgacct tcaataaaat acccccgcag
      361 aaggcatcgg cggtcttgct ctgcgggtgt tcatccgcga aggtacatac ttgggcaatc
      421 gcttcggggg caggcgtgga agtatcctgc aatataatct cacccatgta acgcgctaca
      481 gtttcgtttc gcggatctat tttagctgcg tcctgcagcg cttgactggc ctccgcggag
      541 cggtcaacga agaaatatgt caaccctaaa aggattttta cgcgaacaga ttgcgggaaa
      601 agtgcggatg cctgcttaaa caccaccaga gcagcgtcaa atgtctgatg gcgcaatagt
      661 tccaaaccca gcgcaagatg gtagcgttct tcagacggtt ccagagtgac cgcagcttga
      721 tagctgtgaa ctgccgcaag tgggtctcct cgcttttcct gaatatcacc catgaggctt
      781 tctatagagc cgctatcggc cacacttttg gcgcgttcgg cgctggccaa tgcgtcgtcg
      841 aggccaccgg tacggaaata cgccaaggca agatcataga gtaaatcccc gcggtctggt
      901 tcgagagcac tcgctgccgc aaaatcaatc actgcctcgg aaaacagatt ggcgcggctg
      961 agaatttccg ccaaatgcag acggatatcc acagcttgtt gtggtggcac agtttcggcg
     1021 cgcaaccact tgagtgtttg cgccgggttc gagtgatttt cgacttgatc ctggagacgc
     1081 gcaatacccg ccgcgatgcg caaatcttga tttccgggtg ccaacctggc tgcgttgtcc
     1141 atattttccg ctgctgcggc gaaatggcca agtcctgacg caatggcggc ttttaatcgg
     1201 aagaaggcta ttttttggtc aggatcg
//
LOCUS       DATCBB010000176         1106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_329821, whole genome shotgun sequence.
ACCESSION   DATCBB010000176 DATCBB010000000
VERSION     DATCBB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329821"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VII37_02010"
     CDS             <1..888
                     /locus_tag="VII37_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEY5068522.1"
                     /translation="QRLMDKFPDQAAHISVSLHCELAEILNAYTKIVEREGKLTGLHA
                     YSAARPPHSEGLAIWIASYLANETNCLNINLLHLSSRKAVDAAWTMQQVFPHIAFRRE
                     VTVGHLLLDTDCECAVHAKVNPPIRPREDVEALWQAVLDRKIDWIVSDHACCSAEQKW
                     SKDDPNNIWLAKSGFGGTEYLLSGVLSEGSKRGMSYNHMAELLSWKPAQRFGLPSKGD
                     IAPGYDADIVIVDPHESFVVHAAESESVQGYSPFEGVELSGRVKSTFLRGALVYDSGN
                     IVGPARGKYLRRSAVARTQ"
BASE COUNT          245 a          294 c          329 g          238 t
ORIGIN      
        1 cagcgcttga tggacaaatt ccccgatcaa gccgcccaca tcagcgttag tctgcattgc
       61 gaactcgcag agattcttaa tgcatataca aagatcgtgg aacgagaagg caagctgacc
      121 ggcctccatg cttatagcgc agcgcgccct ccacattccg agggtcttgc catctggatt
      181 gcatcttatc tggcaaatga aaccaattgt ctgaacatca atctgttgca cttgagctca
      241 cgcaaagcag tggatgccgc gtggaccatg cagcaggttt ttccgcacat cgctttccgc
      301 cgcgaagtga cagttggtca tcttcttctc gataccgatt gcgaatgcgc agtgcacgcc
      361 aaagtgaatc cgccaatccg cccgcgcgag gacgtcgagg cgctgtggca ggctgtgctt
      421 gaccgaaaaa tagactggat cgtcagcgat catgcgtgct gttcagcgga gcagaagtgg
      481 tcaaaagacg acccaaacaa tatttggcta gccaaatctg gatttggcgg caccgaatat
      541 ctcctttcag gcgttttgag cgagggcagc aagcgtggca tgtcatacaa tcacatggcc
      601 gagcttttga gctggaagcc agcgcaacgt ttcgggctgc caagcaaggg ggatatcgct
      661 ccaggctatg acgcggatat tgtgatcgtc gatccgcacg aaagtttcgt ggtgcatgcc
      721 gccgaatctg aatccgtgca aggctactca ccgttcgaag gtgtcgaact cagcggtcgg
      781 gtgaagagta cgtttttgcg aggcgctctt gtttatgaca gcgggaacat cgtcgggcca
      841 gcgcgcggca aatatttgcg gaggtccgcc gtcgctcgta cgcaatgaac tgttttaatg
      901 cgcgatgatg cttgactttg aggcataatg agcacggtga cgattacagc ggacgaagaa
      961 ttcaccggcg aaacaccgct gctgatcgtc ggtgccggcg ctgctgggct gtgcgcggcg
     1021 cttgccgcca aggaagccgg agtcgatgcg gtgctgatcg agcgcgacgc cgtgccggcg
     1081 ggatcgacgg cgctgtcggc cgggct
//
LOCUS       DATCBB010000177         1513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_38309, whole genome shotgun sequence.
ACCESSION   DATCBB010000177 DATCBB010000000
VERSION     DATCBB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38309"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1513
                     /locus_tag="VII37_02015"
     CDS             <1..>1513
                     /locus_tag="VII37_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068523.1"
                     /translation="RFQSARDLGFALAALSGTGTGSAAALSKFGDKSRSVPASWLWIA
                     AAIALASLMGLGLALRTGKEHVSQQEFAIPLRGEVNHLAVSADGSLLAYTTPDDKSGK
                     NVINVQKIGSRAVTTLSGTEGASYPFFSPDNQFVGFFADGKLKKIAVSGGSPQVLTLA
                     SNGRGGSWGSKNVILYAPNTGRSIWKINSDGSHAEDLTVKYFSQKESTHRWPLFLPDG
                     ENFLFWAGNFKNVDDDPDSGIYLASINGGDRKRIVLAHSNPAYANGQLVFLEWHRGLV
                     AVKFDPSAGKIEGEKKLIAEGIGFQPSTYYAAFAAGTNGTLVYNPSAGATTSVLAWYD
                     RTGKELSRLGEEGVIANPSLSPDNNYVAADRTDIKASNVDIWIHDVKRGTASRFTFDP
                     AEEVTPIWSPDGQKVVYRLVKVTTIVEMKNLKGTEAEKAIFTSPPPAAFENIPNSWSP
                     DGTQILCTSETADESTYLGLLSTGGAGIKPFLKGNAHYSNGQFSPDGKWVAYAS"
BASE COUNT          365 a          419 c          428 g          301 t
ORIGIN      
        1 agcgtttcca gtccgcgcgc gatctgggct tcgcactggc cgcgctttct ggaactggca
       61 ctggatccgc ggccgcgctc tcgaaattcg gagacaagtc ccgcagcgtt ccggcgagtt
      121 ggttatggat cgccgcagcc atcgcgctgg cgtcgttaat gggactcgga ctagctttgc
      181 gcaccggcaa agaacacgtc agccagcagg agtttgccat tccactgcgc ggagaagtga
      241 atcatctggc agtctccgca gacggatcgc tactggcgta caccacgccc gacgacaaat
      301 ccgggaagaa cgttattaac gtgcagaaaa taggatccag agcggtaact acactgtcag
      361 gcactgaggg agcgagctac ccgttcttct ctcccgacaa tcagtttgtc ggtttctttg
      421 ctgatggaaa actaaagaaa atcgcggtga gcggtggctc gccgcaagtg ttgacgctgg
      481 caagcaacgg gcgcggcggg agctggggca gcaaaaacgt gatcttgtac gcgccgaaca
      541 ccggccgctc catctggaaa atcaactccg acggaagcca tgccgaagac ctcaccgtga
      601 aatatttctc acagaaggaa agcacccatc gctggccgct gtttcttccg gacggagaga
      661 attttttatt ctgggccggc aattttaaaa atgtagacga tgatccggac agcggcatct
      721 acctggcctc catcaacgga ggcgacagaa aaagaattgt actggctcat tccaacccgg
      781 catacgcgaa cgggcaattg gtttttctgg aatggcaccg ggggctggtg gcggtaaagt
      841 ttgatccttc tgcggggaaa attgaaggag agaaaaaact gatcgcggaa ggaattggct
      901 ttcaaccttc cacctattac gctgcgttcg ccgcgggaac taacggaacg ctggtgtaca
      961 accccagtgc cggcgccacc acctcggtgc tggcgtggta cgaccgcact ggaaaagaat
     1021 tgtcacggct cggcgaagag ggtgtgatag cgaatccttc actctctccg gacaacaact
     1081 atgtcgccgc ggaccggacc gacatcaagg ccagcaacgt ggacatctgg attcatgacg
     1141 tgaagcgtgg caccgcctcg cgctttacgt ttgatccagc ggaagaagtc acgccaatct
     1201 ggtcgcccga tggccagaag gtggtgtacc ggctcgtgaa agtgacgact attgtagaga
     1261 tgaaaaatct caaaggcact gaggcggaga aagcaatatt tacctcgcca ccgcccgcgg
     1321 ccttcgaaaa tattccgaat tcctggagtc ccgacggcac gcaaatcttg tgtacctcgg
     1381 agaccgcgga cgaatcaacc tatcttggac tactgtccac cggcggagcc ggcatcaagc
     1441 ctttcctgaa aggaaacgcg cattacagca acggccagtt ttctcccgac ggcaaatggg
     1501 tcgcttatgc ctc
//
LOCUS       DATCBB010000178         1168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_265302, whole genome shotgun sequence.
ACCESSION   DATCBB010000178 DATCBB010000000
VERSION     DATCBB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265302"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..>1168
                     /locus_tag="VII37_02020"
     CDS             48..>1168
                     /locus_tag="VII37_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5068524.1"
                     /translation="MKRHGVAGWLAVFSGISLCVIAALVAAQAQAPPTQPGSTPAAGG
                     AAAAQQPSHSGPKLAEEQFKNIQVLKGIPADQLIPAMQFIAASLGVECDYCHNREKMD
                     SDEKKPKKIARGMMTMMFNIDKTNFEGRREVTCYSCHRGAAMPVGIPEIKEEEAADPT
                     VAGKKPGENVALPKPEELLDKYLAAVGGAVALDKITSRVQTGQLLAFGGQTFPAEVYS
                     KAPDKRISTMHLKGGDSVTAFDGQHGWLGFPGRPAHMMSASENDAARLDADLHFAEHV
                     KTMYSKFNVSAGEKIDGHDTYLVECRTEERTPLRLYMDKESGLLLRLVRYAQSPLGLN
                     PTQIDYADYREADGVKVPFRWTVARPGNRFTIQVAEMKQ"
BASE COUNT          267 a          291 c          395 g          215 t
ORIGIN      
        1 agcagctgct ttgctacaat cctgcgtgtt tgcacgggag atggaatatg aaacggcatg
       61 gtgtggcggg ctggctggcg gttttttcag gcatttcatt gtgcgtgatt gcagcgttgg
      121 tggctgcgca ggcgcaagca ccgccgacgc aacccggctc aactccggca gcgggtggcg
      181 ccgcagcagc acagcagccg agtcactcag ggccgaagct ggccgaagaa caattcaaga
      241 atattcaagt gttgaagggc attccggccg accagttgat tcctgcgatg caattcatcg
      301 ccgcttcgct gggggtggag tgcgattact gccacaaccg cgaaaagatg gacagcgacg
      361 aaaaaaagcc caagaaaatc gcgcgcggga tgatgaccat gatgttcaat atcgacaaga
      421 cgaattttga ggggcggcgg gaagtgacct gctactcgtg ccaccgcggc gcggcgatgc
      481 cggtgggcat tccggaaatc aaggaagaag aagcggcgga ccccaccgtt gcggggaaaa
      541 aaccgggcga gaatgtggcg ctgcccaagc cggaggagtt gctggataaa tacctggcgg
      601 cggtgggcgg cgcggtggcg ctggataaaa ttaccagccg ggtgcaaacc ggccaactgc
      661 tggcttttgg cgggcaaacg tttccggcgg aggtttattc gaaagcgccg gacaaacgaa
      721 tttcgacgat gcatttgaaa ggcggagata gcgtgacggc gtttgacggg cagcacggct
      781 ggctcggctt cccggggcgc ccggcgcaca tgatgtccgc ttcggaaaac gatgcggcgc
      841 ggctggatgc cgatttgcat tttgcggagc acgtgaaaac gatgtattcg aaatttaacg
      901 tgtcggccgg cgagaaaatc gacgggcacg acacttatct ggtggaatgc cgcaccgaag
      961 aacgcacgcc gctgcgtttg tatatggaca aggaatcggg gctgctgcta cggctggtgc
     1021 gttacgcgca aagtccgctg ggactgaatc ccacgcagat cgactatgcg gattatcgcg
     1081 aagcggacgg cgtgaaagtg ccgtttcgct ggacggtggc gcggccggga aatcgcttta
     1141 ccattcaggt ggcagagatg aagcagaa
//
LOCUS       DATCBB010000179         3719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_362417, whole genome shotgun sequence.
ACCESSION   DATCBB010000179 DATCBB010000000
VERSION     DATCBB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362417"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /gene="pruA"
                     /locus_tag="VII37_02025"
     CDS             complement(<1..1542)
                     /gene="pruA"
                     /locus_tag="VII37_02025"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEY5068525.1"
                     /translation="MSEFTNEPFVDFSKPENQRAMEAALKKVEAEFGREYPMYIGGEK
                     IFTTAKMTSTNPSHPKQVIGVFQAATAEHAKQAVEASAKAFETWKRVPAEKRVEIVFR
                     AAEIIRERKFEISALICAEVGKSWIEADADTAETIDFLEFYGREMLRFAGQHPVTPMQ
                     GEKNYLVYIPLGVGVVIPPWNFPAAIAAGMTVASLVTGNTVILKPSEESPTVAAKVVE
                     ILYEAGIPKEALNFLTGAGEIVGNALVKHPKTRFIAFTGSKEVGLLISEAAGKKVPGQ
                     IWIKRAVLEMGGKDATIVDEEADIDAAVEGVALAAFGYQGQKCSACSRAIVSEKIYDT
                     FVEKLVERTKKITVGPSEDPGNYMGPVVSSMAMEGILGYIDVGKKEGKVVLGGERAPG
                     DGYFIQPTIIANVPPKARVAQEEIFGPVLAVIKARDFDHALEIANDTEFGLTGAVYSR
                     NPEKIEKAQEAFHVGNLYLNRKCTGAMVGAHPFGGFNMSGTDSKTGGKDYLLHFLQAK
                     SIAEKV"
     gene            complement(1712..2536)
                     /locus_tag="VII37_02030"
     CDS             complement(1712..2536)
                     /locus_tag="VII37_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5068526.1"
                     /translation="MAPEILADRSLSCRLERAEGLTNAAFVEARARLMPESGAKWISV
                     AGVLAMFDGVESPLTQTFGLGLFQMPTSAELTTLEDFFKERGARIFHEVSPLADKAIV
                     GMLVERGYRPLEMTTVLYLPLRDWTARRDERAGSANVRIVEREEGDIWARTSAEGWSE
                     YKEFAHLMLEMSRVIAAAEGNVLFLAELNGQAAGAAGLSIHEGVALFAGASTIPAWRR
                     QGVQRALLEARFRYAREAAGCDLAMMCTAPGSSSQRNAERQGFRIAYTRTKWTLAG"
     gene            complement(2595..2828)
                     /locus_tag="VII37_02035"
     CDS             complement(2595..2828)
                     /locus_tag="VII37_02035"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY5068527.1"
                     /translation="MDPLRELGRGLLLLGGIAVIVGAFLYFSGRLPLRLGRLPGDIVH
                     RGEHTTFYFPIVTCVLLSIGLSLIFWIFSHFRR"
     gene            complement(2936..3355)
                     /gene="ndk"
                     /locus_tag="VII37_02040"
     CDS             complement(2936..3355)
                     /gene="ndk"
                     /locus_tag="VII37_02040"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY5068528.1"
                     /translation="MALERTFAIIKPDAVSKGQQGEILSRIHKAGFKIVALKTMRLTK
                     EEAGGFYAVHKERPFFGELTDFMSSGKIVALVLEADGAIKKWRDAMGATDPKKAEPGT
                     IRRDLGTSIGNNCTHGSDAPETAAFEIGYFFAGLELI"
     gene            complement(3397..>3719)
                     /locus_tag="VII37_02045"
     CDS             complement(3397..>3719)
                     /locus_tag="VII37_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY5068529.1"
                     /translation="IGTTFLDAIRLFNEDADTHAIIMIGEIGGDAEERASQWIKTNVK
                     KPVVGFIAGQTAPPGRRMGHAGAIISGGQGTAKDKYAAMSAAGIRCVETPADLGSTVA
                     ATLK"
BASE COUNT          867 a         1242 c          781 g          829 t
ORIGIN      
        1 aactttctcc gcgatcgatt tcgcctgcaa gaaatgcagt aaataatctt tcccgccggt
       61 cttggaatcc gtccccgaca tattgaatcc gccaaacgga tgcgccccca ccatcgcgcc
      121 cgtgcatttg cggttcaaat aaagattgcc gacgtgaaag gcctcttggg ccttctcgat
      181 tttctccgga ttccgcgaat acaccgcgcc agtcagtcca aactccgtat cattcgcaat
      241 ttccagcgca tgatcaaagt cccgcgcttt aatcaccgcc aaaaccggtc caaatatttc
      301 ctcttgcgca acgcgcgcct taggcggcac gttcgcaata attgtcggct ggataaaata
      361 cccatctcca ggagcgcgct cgcctcccag caccactttc ccttctttct tccccacgtc
      421 gatataaccg agaatccctt ccatcgccat gctgctcacc accggcccca tataattccc
      481 gggatcctcg ctcggtccta cggtaatttt cttggtgcgt tccaccaact tctccacaaa
      541 cgtgtcgtaa attttctccg acacaatcgc tcgcgaacac gccgaacatt tctgtccctg
      601 gtagccaaac gccgctagcg caacaccctc cactgccgcg tcaatatccg cttcctcgtc
      661 cacaatcgtc gcgtccttgc cgcccatctc caagaccgcg cgcttgatcc agatctgccc
      721 tggaactttc ttccccgccg cctcactgat cagcaacccg acttccttcg agcccgtaaa
      781 ggcaatgaag cgcgtcttcg gatgcttcac cagcgcattg ccaacaattt ctcccgcgcc
      841 agtcagaaaa tttaaagcct ccttgggaat tcccgcttca taaagaatct ctacaacttt
      901 cgccgccacc gtcggtgact cttccgaagg tttcagaatc accgtgtttc cggtcaccag
      961 cgacgccacg gtcatccccg ccgcgatcgc cgccgggaag ttccatggcg gaataaccac
     1021 gcccactccc agcgggatat agaccaggta attcttttcg ccctgcatgg gggtgaccgg
     1081 atgctgcccc gcaaaccgca gcatctcccg cccgtaaaat tccagaaaat cgatcgtttc
     1141 cgccgtgtcc gcatccgctt caatccacga cttccccacc tcggcgcaaa tcagcgcgga
     1201 aatttcaaat ttgcgttcgc gaataatttc cgcggcgcga aatacaatct cgacgcgttt
     1261 ttccgcaggc acccgcttcc acgtttcaaa cgccttggcc gacgcttcca ccgcctgttt
     1321 tgcatgctct gccgtcgccg cctgaaacac cccaatcacc tgcttcggat gagaaggatt
     1381 cgtcgacgtc atcttcgctg tggtaaaaat cttttccccg ccgatataca tcgggtattc
     1441 gcgcccaaac tccgcctcga cctttttcag cgccgcttcc atcgctcgct gattttccgg
     1501 cttggaaaaa tctacaaacg gctcgttagt gaactcggac atgcgtactt ttgactcagc
     1561 ggtagccatt agaaaaaatc tcctcgcgct tcaaccattc attctactgt gttgggaaac
     1621 cgccggacaa ccacacacaa tcacagatgt agtaccactt caatcacgaa aatgtcatta
     1681 gagccgaaga gcctgccaaa caattcatcg tctatccagc aagagtccac ttcgtgcgcg
     1741 tgtacgcaat ccgaaacccc tgccgctcgg cgttacgttg cgacgaactc cccggcgccg
     1801 tacacatcat cgccaggtcg catccagcag cctctcgtgc gtatcgaaat ctcgcttcca
     1861 acagcgcgcg ttgcacaccc tgcctccgcc acgccggaat ggtgctggcc cccgcaaaca
     1921 gcgccacgcc ctcatgaatc gacaggcccg cggccccagc cgcttgcccg ttcagttccg
     1981 ccaaaaacaa tacgtttcct tccgccgccg ctatcacgcg agacatttcc agcatcaggt
     2041 gcgcgaattc cttgtactca ctccaacctt cggcggaggt gcgtgcccaa atatcccctt
     2101 cttctctttc aacgatgcgc acatttgccg agccagcgcg ttcatcgcgc cgagccgtcc
     2161 agtcacgcaa cggcagatac agcaccgtgg tcatttccag cggtctgtaa ccccgctcaa
     2221 caagcatccc gacaatcgcc ttgtccgcca gcgggctcac ttcgtggaaa atgcgcgctc
     2281 cgcgctcttt aaaaaaatcc tcgagcgttg tcaactccgc gctggtgggc atctgaaaca
     2341 atccaagtcc aaatgtctgc gtcaagggcg actccacccc atcaaacatc gccaaaaccc
     2401 cggcaaccga aatccactta gcccccgact ccggcattaa gcgcgcacgg gcttccacaa
     2461 atgccgcgtt agtcagccct tcggcgcgct ccagccggca agacagagag cgatctgcaa
     2521 gaatttcggg agccatcgaa gtttccgaac gttatcacaa aggcttagcg gattgatcta
     2581 tcaagcgaaa ttatttagcg tcgaaagtga gaaaaaatcc aaaagatcaa cgacagtccg
     2641 atgctcaaaa gcacgcaggt aacaatagga aaataaaatg tagtgtgttc accgcgatga
     2701 actatatcgc caggcaggcg ccccagccgc aacggcaaac gtccgctgaa atacaaaaac
     2761 gcaccgacaa tcactgcaat cccgccaagc aataaaagcc ctcgtccgag ttcacgcagc
     2821 ggatccataa ctcaaattct acgccgaggg tcccggcagg caataccatg acggcgggag
     2881 aacttcagta gtagaagcac tagactaacg gcgtaccgcc cctgataaaa ttccactaaa
     2941 tcaattcaag tcccgcaaaa aaatatccaa tttcaaacgc tgcggtctcc ggagcatccg
     3001 atccgtgagt acaattattc ccgatacttg tcccgagatc gcgtcgaatc gttcccggct
     3061 cggccttctt gggatcagtc gcacccatcg catcccgcca tttcttaatc gccccgtcag
     3121 cttccaaaac cagagctaca attttccctg aactcataaa atccgttaac tcgccaaaaa
     3181 atggccgctc cttatgcacg gcataaaatc cgccggcctc ttcctttgtc aggcgcatcg
     3241 tcttaagcgc cacgattttg aatcccgctt tatgaatgcg tgaaagtatt tcgccctgct
     3301 gccctttact aactgcgtcc ggcttgataa tcgcaaatgt gcgttccagt gccacgaaat
     3361 tcctcccaat tacagattaa agtttgttcc gtatcgttac ttcagtgtgg ccgcaaccgt
     3421 tgaacccaga tccgctggcg tttccacaca gcgaattccc gcggcgctca tagcagcgta
     3481 cttatccttc gctgtgccct gtccgccgct gattatcgcc ccagcgtgac ccatgcgcct
     3541 tccgggcggc gcggtttggc ccgcgatgaa tcctacaacc ggcttcttca cattcgtttt
     3601 gatccactgc gaggcgcgtt cttcagcatc gccgccaatt tccccgatca tgataatcgc
     3661 gtgcgtatcg gcatcttcat tgaaaagtcg aatcgcatcc agaaaagtcg tcccgataa
//
LOCUS       DATCBB010000180         1388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_200291, whole genome shotgun sequence.
ACCESSION   DATCBB010000180 DATCBB010000000
VERSION     DATCBB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200291"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VII37_02050"
     CDS             <1..1069
                     /locus_tag="VII37_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5068530.1"
                     /translation="DKTRAQQPDTLREILSQPQAWNESLSDFKSSAVLQTLLDKTASR
                     SEWLFVGCGTSFYLAEAAAASWHILTGQPARAVPASEIMLFPALNLARRADLQAVVIS
                     RSGSTSEAVRAAALLRQEHRVPTIGLTCSPKSPLESACEFTIQLSSAEEKSMVMTRSF
                     TAMLLVMQYFAGVKSGAKELCSSLESLAGRFDSSLRSFSEQVAAFTAQRSFEDYIFLA
                     QGPFVGIAHESSLKITEMSCSYSQPYHTLEFRHGPKAIVGPETCLTFFVSETGKDAEC
                     EVLAEMKELGGVIIAVCNHANKTIRASSDLVFELGFDVPEIAALAPYVVPAQLLGCYT
                     GMKKGLNPDAPKNLSRVVILD"
     gene            1071..>1388
                     /locus_tag="VII37_02055"
     CDS             1071..>1388
                     /locus_tag="VII37_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY5068531.1"
                     /translation="MERSPAKFDVSVVGELNLDIILSGLPEKLQLDHEHLAAGLNVTL
                     GSSSAIFAHNFALLGNQVGFSSAVGSDSFADTCLQRLGESGVDISGVRRVPDRQTGLT
                     VILP"
BASE COUNT          274 a          389 c          405 g          320 t
ORIGIN      
        1 cgacaagacg agagcgcaac aacccgacac actgcgggaa attctgtctc aaccgcaggc
       61 ctggaatgaa tcgctgagcg atttcaagtc cagcgctgtg ctgcagaccc tgcttgataa
      121 aacggcgtcg cgcagtgaat ggttgttcgt cggctgcggc accagctttt acctggcgga
      181 agcggcggct gcgtcgtggc atattcttac cggtcaaccg gctcgcgctg ttccggcttc
      241 cgagatcatg ctgtttcccg cgctgaatct ggcgcgccgc gccgatttgc aggcggtagt
      301 gatttcccgc tcgggcagca catcggaggc cgtgcgcgcc gccgctttgc tgcggcaaga
      361 acaccgcgtt cccacgatcg gtttgacttg ttcgccgaaa tctcctctcg aatccgcgtg
      421 cgaattcacc attcaactgt cttcggcgga agagaaaagc atggtgatga cgcgctcgtt
      481 caccgccatg ctgctggtga tgcagtactt tgcgggagtc aagagtggcg cgaaggaact
      541 ctgctcatcg ctggaaagcc tggcgggccg ctttgattct tcgctgcggt ctttctctga
      601 acaggtggcc gcatttaccg cgcagcgaag ctttgaggat tacatttttc tggcgcaggg
      661 accttttgtg ggaattgctc acgagtcttc gttgaaaatt acggagatgt cctgctcgta
      721 tagccagcca tatcacacgc tggaatttcg ccacggtcct aaagcgattg tggggccgga
      781 aacttgcctg acgttttttg tgagcgaaac gggaaaagat gcggaatgcg aagtgctcgc
      841 cgaaatgaaa gaactcggcg gcgtgattat tgcggtctgc aatcacgcca acaaaacgat
      901 tcgcgcttcg agcgatctgg tgttcgaact gggattcgac gttccggaga tcgccgcgct
      961 cgcgccttac gttgtgccgg cgcagctcct gggttgctac accggcatga aaaaaggact
     1021 caatcccgat gcgcctaaaa atctaagccg cgtggtcatt ctcgattaac gtggagcgct
     1081 cgccggctaa attcgatgtt tccgtcgtcg gcgagctgaa cctcgacatc attctgtccg
     1141 gcctgccaga gaagttgcag ctcgaccacg aacatctggc cgcgggtcta aatgtcacgc
     1201 tgggaagttc ttcggcgatc ttcgcgcaca atttcgcttt gcttggcaat caggtcggct
     1261 tcagctccgc ggtgggaagc gactcgttcg cagacacctg cctccagcga ttgggagagt
     1321 ctggagtgga tatttccggc gtgcggcgtg tcccggaccg acaaaccggc cttaccgtaa
     1381 ttcttccg
//
LOCUS       DATCBB010000181         1366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_135765, whole genome shotgun sequence.
ACCESSION   DATCBB010000181 DATCBB010000000
VERSION     DATCBB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135765"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..>1366)
                     /locus_tag="VII37_02060"
     CDS             complement(116..>1366)
                     /locus_tag="VII37_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY5068532.1"
                     /translation="PAISILEHWALFVFMAWALGASFAMLRLAAGFWRLHRLRQSCTP
                     IDIADLNPTLHNTWLQSTSLRNTLATIRSSNAAGFFKTSKPITIATSEDLRVPAAIGF
                     WSRTIVLPAWAMRELPPEDLNVILLHEFAHLRRGDDWTNLIQKIVRSLFFFHPAVWWI
                     ENRLSVEREMACDDAVLAQTANPHAYATCLVSLLEKSLAHRGWTMAQAAVHRAHEASL
                     RLAQILNKNRPAATKVWSPALGIAGVFSVLCLVAAPQAPRLVAFDHPPNVLAPQTTAA
                     LTNGGPGVPARQASAQIVRATVSAKTVPTTSLAVVHQGDHQDAHRSAARPHNFTERQL
                     APAVVVARLNADAPQLVEARATDQEPGPQIQTQFQTLIFIETTPFTPDSPSMGQAVMV
                     RVWQITWISTMPQTGARVPVANSI"
BASE COUNT          296 a          361 c          399 g          310 t
ORIGIN      
        1 ttgcaccggc tttacaattt tctatccacc tatacatttt tgtttctcct tattagactt
       61 gcttaaagtg tgtgtaacta attatctagt tgatccagcc aaaaaaattt tactgctaaa
      121 tcgaatttgc cacaggcact ctcgcccccg tttgcggcat tgtgctgatc cacgttattt
      181 gccagaccct caccattact gcctgtccca tcgatggtga atctggagtg aacggagtgg
      241 tttcaataaa gatcaaggtt tgaaattgag tttgaatttg cggacctggt tcctgatctg
      301 ttgcgcgcgc ttcaaccaat tgcggcgcgt ccgcattcag tcgggccacg acgacagcgg
      361 gcgcgagctg acgttcggta aaattatgcg ggcgcgcagc ggagcgatga gcgtcttgat
      421 ggtctccttg gtggactacc gcgagggaag tcgtagggac tgtcttggcg ctaaccgtcg
      481 ctctaactat ctgggccgat gcctgccggg cggggacgcc cggccctcca ttcgttaatg
      541 cggcggtagt ctgcggggcg aggacgtttg gcgggtgatc gaatgccacc aatctcggcg
      601 cttgcggcgc ggccacgaga cagagcacag aaaaaacgcc cgctatgcct aaagccggag
      661 accaaacctt cgttgctgcc ggacggtttt tgttgagaat ctgcgccagc cgaagggaag
      721 cttcgtgggc gcgatggacg gcggcctgag ccatcgtcca tccgcgatgc gccaggcttt
      781 tttccaagag agaaaccaga cacgttgcgt aggcatgagg atttgcggtt tgggccagca
      841 cagcatcatc gcaagccatc tcgcgttcta ccgagagccg gttttcaatc caccacactg
      901 cgggatgaaa gaaaaataaa gagcggacaa ttttctggat gagattcgtc cagtcgtcgc
      961 cgcgccgtag gtgggcgaac tcgtggagca ggattacgtt gagatcttcc ggaggaagtt
     1021 cgcgcattgc ccaggctggc agcacgatgg tccggctcca aaacccgatt gccgccggca
     1081 cgcgcaagtc ttcggacgtg gcgatcgtga ttggctttga ggttttgaaa aatcctgctg
     1141 catttgagga gcggatcgtg gccaaggtgt tccgtagcga tgtgctctgt agccacgtgt
     1201 tgtgcagcgt cggattcaga tccgcaatgt cgatcggcgt gcaactctgg cgcaggcgat
     1261 gcaggcgcca aaatccggcg gctaaacgca gcatcgcaaa cgatgctcca agagcccacg
     1321 ccatgaagac gaagagcgcc caatgctcca agatggaaat cgccgg
//
LOCUS       DATCBB010000182         1346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_395416, whole genome shotgun sequence.
ACCESSION   DATCBB010000182 DATCBB010000000
VERSION     DATCBB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395416"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..>1346)
                     /locus_tag="VII37_02065"
     CDS             complement(355..>1346)
                     /locus_tag="VII37_02065"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5068533.1"
                     /translation="GDYVIQNFRFRSGETLPELKIHYRTIGTPKMNSSGVVLNAVLIG
                     HGTGGAGTQFLSPQFANVLFGPGQLLDATRYFIILADGIGHGGSSKPSDGLHARFPHY
                     TYDDMVAAHYELLTKKLGVSHMRLVMGTSMGGMQTWVWAETYPDFMDALMPLASLPVE
                     IAGRNRIWRRMAMDAIRDDPEWLGGEYKTEPMRALTSAENLLLFVGSAPLFWQKQWPT
                     RDAADKSYEDRVKVAVARLDANDLLYQLDASREYNPAPKLETIKAPLVAINSADDVIN
                     PPELGIIEREIKRVKRGRFILLPITDLTRGHGTHTLPAIWQRYLKELLAESEK"
BASE COUNT          282 a          373 c          384 g          307 t
ORIGIN      
        1 gaatcgggcg attatgtaat ccgcgtgttc atgatgcgat cggcggccag aagaaaaggc
       61 acggcggcaa atttcacttt gagcatttcg attcgatagg gaattggtcc ttgccgctcc
      121 gcgggtttgt tcaagcgcca aaggcgcgaa atgtaaaagc ctgggccaac ggcccaggtt
      181 aggtcatctt gtaaaagaca atgagcgctg aaggcgcgga atagaattga gttctcctca
      241 caaattattt cgcgccttca gcgctcagac ttgttttgcg gtgattgatc caggggcgac
      301 gcttcgctag cccctggcta ttacatctca cgccttcagc gtgaacatct ctctttactt
      361 ttccgactca gccagcaact ccttcaaata cctttgccag attgccggca gcgtgtgcgt
      421 gccgtggccg cgcgttagat cggtgatcgg cagcagaata aagcggccgc gcttgacgcg
      481 tttgatttcc cgctcgatta ttcccaactc cggcgggttg atcacgtcgt cggcggaatt
      541 gatcgccacg agcggcgctt tgatcgtctc gagtttcggc gccggattgt attctcgcga
      601 agcgtcgagc tgataaagca gatcgttggc atccagacgc gcgactgcca ctttcacgcg
      661 gtcttcgtag gacttgtcgg cggcgtcacg tgtgggccac tgcttctgcc agaataaagg
      721 cgcgctgccg acaaataaga ggagattttc agctgaagtc agcgcccgca tcggctcggt
      781 tttgtattcg ccgccgagcc actcgggatc gtcgcgaata gcatccatgg ccatgcggcg
      841 ccagatgcga ttgcggcccg cgatctcgac cggcagactc gccagaggca tcagggcatc
      901 catgaagtcc ggataggtct cggcccaaac ccacgtctgc atgccaccca tcgatgtgcc
      961 catgacgaga cgcatgtgac ttacgcccag cttctttgtc agcagttcat aatgcgcggc
     1021 caccatgtcg tcgtacgtgt aatgcggaaa gcgagcatgc aggccgtcgc tgggtttgct
     1081 cgaaccgcca tggccgatgc cgtcagcgag aatgatgaag tagcgggtcg cgtcaagtaa
     1141 ctgtcccgga ccaaacagga cattggcgaa ctgtggcgag agaaactgcg ttcccgcgcc
     1201 gcccgtgcca tgaccaatca gcacggcatt cagtacgaca cctgagctat tcattttggg
     1261 tgtgccgatg gtgcggtaat gaattttcag ttctggcagt gtttcgccgc tgcgaaaacg
     1321 gaagttttga atgacgtaat cgccct
//
LOCUS       DATCBB010000183         1529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_395424, whole genome shotgun sequence.
ACCESSION   DATCBB010000183 DATCBB010000000
VERSION     DATCBB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395424"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VII37_02070"
     CDS             complement(<1..190)
                     /locus_tag="VII37_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068534.1"
                     /translation="MRIGICVLLLMASIVSGGRSAAQSQTLEVKKSVFGKTKDGREAH
                     LYTLANKSGMQVVISDFGG"
     gene            complement(309..>1529)
                     /locus_tag="VII37_02075"
     CDS             complement(309..>1529)
                     /locus_tag="VII37_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5068535.1"
                     /translation="VFVNYLDRVNISVSQQALHDAFGVSTIIFGYLLSAYSWTYAAMQ
                     LPSGVLLDRFGVRKVGCISTFLWSVASFCSAAASGIGSFFVARLLLGIGEAPTFPGNS
                     KAVGYWFPDRERSLATAFFDSAAKLGPAIGVPFIGILLLHFGWRWSFAATALISLVYL
                     ALFYWLYRNPSEDAKLSDAEREFIVRGGAQPEDAGKSGKGASILYLLSQTKVVGLVLG
                     SAAYNYTFYLLLTWLPSYLSTALHVNLRDSVLYSSVPWIFAAFTDFVIGGWMVNELIR
                     RGFDSSRVRQIVLVLGTALGLGILGAAQAHTPAVAIFWISISIGGLAAAAPVGWSIPS
                     LIAPAGSVGRLGGILNFGNQLSAIAAPIVTGYVASATRSFAWAFAAAAFFLLIGICGY
                     VFLLGRIEPIPDPA"
BASE COUNT          428 a          468 c          351 g          282 t
ORIGIN      
        1 tgccgccaaa atcacttatg accacctgca tgcccgactt gttcgcgagc gtatagaggt
       61 gcgcttcacg gccatctttg gtcttcccga acacgctttt cttcacctca agagtttgcg
      121 attgcgccgc tgagcggcca ccactaacta tcgatgccat caacagtagc acgcagattc
      181 caattctcat ttgccctctc tttaacgacg attacacctt ctgcctaatc tgcagtctat
      241 aagtgccctc aaagttgtac gccactccac gattcttttc cacgtgcagc gagaagttta
      301 ttctgtattc aagcaggatc gggaataggt tcaatccgcc ccaacaaaaa gacatagcca
      361 cagatcccga tcaacaagaa aaacgctgcc gcagcaaaag cccacgcaaa agaacgtgtg
      421 gcggaagcga catagccagt gacgatcggt gcggcaatag ccgacaattg atttccaaaa
      481 ttgagaattc ctcccaatct tccaacgctg cccgccggcg caatcaagga cggaatagac
      541 caacccaccg gggcagccgc cgccagcccg ccaatggaaa tgctgatcca aaatattgcc
      601 accgcaggcg tgtgcgcttg cgctgcgcct aaaatgccca gtccaagcgc ggtcccgagc
      661 accaagacga tctgacgcac gcgcgaggaa tcgaacccgc gccgaattag ttcattcacc
      721 atccaaccac cgataacaaa atcggtgaac gccgcgaata tccatggaac gctggagtac
      781 aacaccgaat cgcgcagatt cacatgcagt gcggtagaca aatagctggg caaccaggtc
      841 aacagcaaat aaaaggtgta gttgtatgcc gcggagccca acaccaggcc gacgacctta
      901 gtttggctca acaaatataa aattgacgct ccttttccac tcttgcccgc atcttcaggc
      961 tgcgcgcctc cgcgcacgat aaattcacgc tcggcatcgg aaagttttgc gtcttcactg
     1021 ggatttctgt acagccagta aaacagtgcg agatagacca ggctaatcag cgccgtggct
     1081 gcgaaactcc accgccatcc aaagtgcaaa agaaggatac cgataaaggg cacgccgatc
     1141 gcaggaccca gcttggcggc cgagtcaaaa aaagctgtag ccaaacttcg ctcacggtcc
     1201 ggaaaccagt agcccaccgc tttggaattt cccggaaacg tcggcgcctc accgataccc
     1261 agcaatagcc gcgccacaaa aaagcttcca atcccagaag ccgcggccga acagaatgaa
     1321 gccacgctcc acaaaaaggt gctgatgcac ccgactttgc gcacaccaaa ccgatcgagc
     1381 aaaactccgg atggaagctg catcgccgca tacgtccagc tgtaggcgct gagcaaatac
     1441 ccaaagataa tcgtgctcac gccaaacgcg tcgtgcagtg cctgctgcga aacggaaata
     1501 ttgacgcggt ccagataatt cacgaacac
//
LOCUS       DATCBB010000184         1047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_460466, whole genome shotgun sequence.
ACCESSION   DATCBB010000184 DATCBB010000000
VERSION     DATCBB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460466"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VII37_02080"
     CDS             <1..479
                     /locus_tag="VII37_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY5068536.1"
                     /translation="TVYTDFSLRVLMFLALKGDGLATIAEIAKAYGISKNHLMKVAHQ
                     LGIAGYVETVRGKGGGLRLARRPQDIVIGEVVRRTEPDMALVPCFAPVDASCTIFSSC
                     ALRGVLSEARDAFLSALDGHTLLDLVRPRASLRKLLSFNPNGRTAAAQTSRSTRRA"
     gene            560..940
                     /locus_tag="VII37_02085"
     CDS             560..940
                     /locus_tag="VII37_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068537.1"
                     /translation="MLRWKTGRSRIVKFAAKFFTAEIRAWHGEEPLWKVFWVYGAATS
                     VTIVTLYIVAFYDGHMALRQVLLPCFAAYTAWILVSVWRCASNTNEKLWSTLARFLTV
                     AWAGNTILVLIFLQLNLLRIYLQH"
BASE COUNT          204 a          293 c          300 g          250 t
ORIGIN      
        1 taacggtcta tacggacttc tcgttgcgcg tgctcatgtt ccttgcgctc aagggcgacg
       61 ggctcgccac catcgcggag atcgccaagg cttatggaat ctcgaaaaac cacctgatga
      121 aggtggcgca tcaacttggt attgcgggat acgtggagac ggtgcgcggc aagggcggcg
      181 gcctgcgcct tgcgcggcgg ccgcaggaca tcgtcatcgg cgaggtggtg cgacgcaccg
      241 aacccgacat ggcgctggta ccttgctttg ctccagttga tgcatcgtgc acgatttttt
      301 cgagctgcgc gctgcgcggt gttctttcgg aagcgcgcga cgctttcctt tcggcgcttg
      361 acgggcacac cttgttggac cttgtccgtc cgcgtgcgtc gctccggaaa ttattatcct
      421 ttaatccaaa cggcaggacc gcagcagcac aaacctcgcg atccacccgg cgggcatagt
      481 tatgttacgc atgctcgctc gaatacttgg aggacaagct cgcaaccgcc ccgtaattag
      541 atcggagcct tcagcagcca tgttgcgttg gaaaacgggc agatcgagaa ttgttaaatt
      601 cgcggccaaa tttttcaccg cggagattcg tgcgtggcac ggcgaggagc cgctctggaa
      661 agtgttctgg gtctacgggg ctgccaccag cgtcacgatt gtcaccctct acatcgtcgc
      721 gttttatgac ggtcacatgg cgctgcggca ggttctcctg ccttgcttcg ccgcctacac
      781 agcctggatc ctggtctcgg tgtggcgatg cgcaagcaac accaacgaaa aactttggag
      841 tacgctggcg cgcttcctga cggtggcctg ggccggaaat acgattcttg tcctgatctt
      901 cctccaactc aaccttttga gaatatattt acagcactag gcaaatgaca taccgcacgt
      961 gcgccaggat tgacgggata tcaccggcat gaccgaattg ttgacgggtt ggaatgagct
     1021 aatttttgca tttgtcctgt tcttttt
//
LOCUS       DATCBB010000185         1487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_298125, whole genome shotgun sequence.
ACCESSION   DATCBB010000185 DATCBB010000000
VERSION     DATCBB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298125"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VII37_02090"
     CDS             <1..824
                     /locus_tag="VII37_02090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5068538.1"
                     /translation="KVNLEASDGEVEDKFTELKSPFTEDEFQRQLKERGVSVDDLKRD
                     LRRQLSIQKLLNREVVAKISITDQDVAEFYNANKAQFNVAEPQFRIAQIVVTPRKEPQ
                     IRNRKNDDATNEAEAQRKVKMLMDRLNSGADFAQLAMDYSEDLNSAATGGDMGYVPES
                     ALNQSDPALKKLVLGMKPGQVSQPIQLKEGYRILKLITRESPGLRGLAEPQVQQTIRD
                     TLRNRKEQLLRAAYLAIARDEARSTNYLAQQVIETAGKLPDGGSADAAVKPVGQQ"
     gene            1096..>1487
                     /locus_tag="VII37_02095"
     CDS             1096..>1487
                     /locus_tag="VII37_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5068539.1"
                     /translation="MSTSADVLIIGAGLAGLCCAKRLTEAGITFEIVEGSDGIGGRVR
                     TDEVDGFLLDRGFQVLLTAYPEARNILDYSRLDLKSFSPGALSWFAGRMNPIVDPWRM
                     PGMWREALRSEFGTLGDKLRMARLRSRL"
BASE COUNT          351 a          351 c          459 g          326 t
ORIGIN      
        1 agaaagtgaa tctggaagcc agcgatggcg aagtggaaga taagttcacg gagttgaaaa
       61 gcccgtttac ggaggatgaa ttccaacggc aattgaaaga gcgcggcgtg tccgtcgacg
      121 acttgaagcg ggatctgcgg cggcagcttt cgatacagaa gcttttgaac cgcgaagtgg
      181 tggccaagat ttcgattacc gaccaggacg tagcggaatt ttacaacgcc aataaagcgc
      241 aatttaatgt ggcggagccg caattccgga tcgcacagat tgtggttact ccccgaaaag
      301 aaccgcaaat tcgcaaccgt aagaatgatg atgcgacaaa tgaggcggaa gcgcagcgta
      361 aagtaaagat gctgatggac cggctgaaca gcggtgcaga tttcgcgcaa ctggcaatgg
      421 attattcgga agacctgaat tcggcggcca ccggcgggga tatggggtat gtgccagagt
      481 cggctttgaa tcagtctgac ccggctctta agaagttggt gctgggcatg aagccgggac
      541 aggttagcca acccattcag ttaaaagaag gatatagaat tttgaaattg attacgcgcg
      601 agtctcccgg actgcgcggg ctggcggagc cgcaagtgca gcaaacgatc agggatacgc
      661 tgcgaaatcg caaagaacaa ttgttgcgcg cggcttatct ggcgattgcg cgggatgaag
      721 cgcggtccac gaattacttg gcgcagcagg tgattgagac ggcgggtaaa ctgccggatg
      781 gtggcagcgc cgatgcggct gtgaagcccg tgggccagca gtagacttct agttactttt
      841 ggcgagcttt tcaagcgaag cccgcgacca tatggcataa cgagtctccc aatcttgcga
      901 gtaccttcaa atcgtcaatt cagacggtca cttcctccag ccaggtgacc cggccttcca
      961 acaaacctcg caacgcacgt tgcctgatta cttcagaccc gctcagactt gcgcgaaatc
     1021 ccgccgatgc ctcaggatta gggttcactt tttgtcaatt tgattgcgat tgggctccag
     1081 ggggaatctg agagcatgtc gacgagtgcg gacgttttaa ttattggcgc cgggctggct
     1141 ggattgtgtt gcgcgaagcg cctcaccgaa gcaggtatca catttgagat tgtcgaaggg
     1201 tcggacggga ttggcggccg ggtgcgcacc gacgaagtgg acggattttt gctggaccgc
     1261 ggatttcagg ttttgctcac cgcgtatcct gaagcgcgca acatcctgga ttactcccgg
     1321 cttgatctca aatctttttc tcctggagca ttgtcgtggt ttgccgggcg catgaacccc
     1381 attgttgatc catggcggat gcctgggatg tggcgagagg cgttgcgctc ggaatttggc
     1441 acgctgggag acaaattgcg gatggcgagg ctgcggagtc gtctgaa
//
LOCUS       DATCBB010000186         4413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_265686, whole genome shotgun sequence.
ACCESSION   DATCBB010000186 DATCBB010000000
VERSION     DATCBB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265686"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VII37_02100"
     CDS             <1..1252
                     /locus_tag="VII37_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068540.1"
                     /translation="GAKPQYTMAEYNSYQAAAADKNPASQVKALDDFVAKYPNSALLI
                     YIYPLYYQAYSQLKNYPKVIEYADKVAGLGDKMDPGARYQALYARAFAYNAMIVDPAN
                     AKTAATDPALAKSALDGAVAGLKALDDVKKPDSMSDDAFATQKKQIQIYFHGTAAQAA
                     MVLKDNNAAVEHYKAVLTLNPDDAITSYRLGLAYMAMAPPQQMNAFWSIARAVTAKGA
                     TQAQSAKVKDYLRKLIVNYQQAACDNLTDAELNELLQLAGSSPDRPDSYKLPSADDLT
                     AARKDMTMRSVMEDLKAGGDKAKVTWLAACGLEFPEVPGKVIEVTPGTDAVQLKVAFV
                     TSEAEFESASTPNMDVKVVAQPEAGKLEKNGAVHFTGTLLSFDPDPAFMLHFDKAKVK
                     EEDLPKEGTKKPAARRPAAKKPAQ"
     gene            complement(1344..1571)
                     /locus_tag="VII37_02105"
     CDS             complement(1344..1571)
                     /locus_tag="VII37_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5068541.1"
                     /translation="MKDQLEGLVNQMVERGILFDEAVGEFEKRFIKRVLDGAKGNQSR
                     AAVALGIHRNTLSRKIGEYKLDHNGHSKSRY"
     gene            complement(1648..2544)
                     /locus_tag="VII37_02110"
     CDS             complement(1648..2544)
                     /locus_tag="VII37_02110"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY5068542.1"
                     /translation="MPAIPASRILRETHFAGLEPSARGKVRDIYDLGDKLLIVATDRL
                     SAFDVILPTPIPDKGRVLTQLSLFWFDLFKDVIPNHVLSADHFPAPFNAYRDELAGRS
                     MIVRKTEPLPVECVVRGYVSGSGWKDYAATGKICGIELPPGLRESDRLPAPIFTPSTK
                     AAAGHDQNISFEQVVSLIGQELATQVKAVSIDLYQRAAAYAEPRGVILADTKFEFGLV
                     DGELMWIDEALTPDSSRFWPAAQYKPGGAQPSFDKQFVRDYLERIRWAKVPPAPELPP
                     EVVAATSEKYLEAFRILTGHAL"
     gene            complement(2801..>4413)
                     /locus_tag="VII37_02115"
     CDS             complement(2801..>4413)
                     /locus_tag="VII37_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068543.1"
                     /translation="VTGGRADEAGPLGMKRELRALDAEVMQLEHETTARQATLEALTA
                     DLQLAQKALEDVDQQQREADRNVFASKHRHQQMQSELARLGLELTVCQNELRRIRQDV
                     EQARTRADRAKNEHATATANRAHAEAERSRLTEQLSELRGSIESDQNELAAGRAELAA
                     MNERLSAAEALATRLAEEHSELVRREASLHQQLNSVTEELASLTQQSEELALQLEGLR
                     AEKLRLETRQHEMEQEWDGGRNRVAQMEDHLRMGRQSLQELREQRSHAEIARARNDSD
                     RQHLRETCMAEVNAQPEDLLATETAFMSGEELAAAEISYREMKERIDSMGAVNMMALE
                     EFNECDQRFTFLTRERDDLLKSIQDTQQAIAELDAATKEKFEHAFHAINKSFSEAFHT
                     IFGGGMAEMRLTEADSSGDAGIDIVASPPGKRLQNILLLSGGEKAMTALALLIAIFRY
                     QPSPFCILDEVDAPLDEANVGRFTRLIGDMSDQTQFIIVTHNRKTMEMGSVLYGVTMQ
                     EPGVSKLVSVRWEGDAEPKQRAAAASAA"
BASE COUNT          964 a         1145 c         1307 g          997 t
ORIGIN      
        1 gggcgccaag ccgcagtaca ccatggcgga gtacaactcc taccaggcag ccgctgcgga
       61 caaaaatcct gcgtcgcaag tgaaggcgct agatgacttt gtggccaagt atccgaactc
      121 cgcgctgctg atttatatct atccgttgta ttaccaggct tacagccagc taaaaaatta
      181 tccgaaggtg attgagtacg ccgacaaggt tgcagggtta ggcgataaga tggatccggg
      241 tgcgcgctac caggcactgt atgcgcgcgc gtttgcttac aacgcgatga tcgtcgaccc
      301 ggccaacgcc aaaacggccg cgaccgaccc ggcgctggcc aagagcgcgc tggatggcgc
      361 ggttgccgga ttgaaagctc tggacgatgt gaaaaagcct gactccatga gtgacgatgc
      421 atttgctacg cagaaaaaac agattcaaat ttatttccat ggaacggcgg cgcaggccgc
      481 gatggtatta aaggataaca atgccgcggt ggagcattac aaggcggtgc tgaccttgaa
      541 tccggatgat gccatcacga gctaccgctt gggcttggcg tatatggcca tggcgcctcc
      601 gcagcaaatg aatgcgttct ggtccatcgc tcgcgcggta actgctaaag gcgcaacgca
      661 agcgcaatcc gccaaggtga aagattatct gcgaaaactc atcgtaaatt atcagcaagc
      721 cgcttgcgac aatctgaccg atgcggaact gaacgaattg ctgcaattgg ctggcagttc
      781 gccagaccgt ccagacagtt ataaattgcc gagcgcggat gaccttacgg cagcacgcaa
      841 ggatatgacc atgaggtctg tcatggaaga tctcaaagct ggcggcgaca aagcgaaggt
      901 tacttggctg gcggcttgcg gcctggaatt cccagaggtg cccggcaaag tcatcgaggt
      961 tacgccgggg accgatgctg tgcagctgaa agttgcgttc gtcaccagtg aagctgaatt
     1021 cgaatcggcc agcacaccta acatggacgt gaaagtcgtt gcgcagcctg aagcgggcaa
     1081 actggaaaag aacggcgctg tgcactttac cggcacgcta ctgtcgtttg atcctgatcc
     1141 tgcgttcatg ttgcactttg acaaggctaa ggtgaaggaa gaggatctgc cgaaagaagg
     1201 aaccaagaag cccgcggccc gacgtccggc ggctaagaaa ccggcgcagt aattaacgtc
     1261 tagcgtttta aaacggctct ccttaactgg ggagccgttt tttattggcg gagtatattt
     1321 ttcgtcgggc ttgagatttg cgtttagtac cgggatttgc tgtggccgtt gtggtccagt
     1381 ttgtattcgc cgatttttcg gctgagggtg ttgcgatgga tgcctagcgc gaccgcggcg
     1441 cgagattgat tacctttggc accatccaga acccgtttga taaaacgctt ttcgaattcg
     1501 cccactgctt catcgaataa aataccgcgt tccaccatct gattgacgag cccttccagt
     1561 tgatctttca cgttggacgc tccatgtgaa aaaactcaag tttgctaaag tctttgctcc
     1621 gcctgggtcg ttgctccagg cgatccgcta tagggcatgg cccgtgagaa tgcggaaagc
     1681 ttccaggtac ttttcgctgg tggcggccac aacttcgggc ggtaattcgg gcgctggggg
     1741 aactttggcc cagcggatgc gttcgagata atcgcgcacg aattgtttgt cgaaagaggg
     1801 ctgggcgccg cctggcttat attgggctgc gggccagaag cgcgaagaat cgggcgtaag
     1861 agcttcatcg atccacatca actcgccatc gaccagaccg aattcaaatt tagtgtcggc
     1921 aagaatgacg ccgcgcggct cggcgtaggc tgcggcccgt tggtaaaggt cgatgctgac
     1981 cgctttgact tgagtagcta gctcctggcc gattaaagaa actacctgtt cgaacgaaat
     2041 gttctggtcg tggcccgcgg cggcttttgt agagggcgtg aagattgggg cgggtagccg
     2101 gtctgattct cgcaggccgg ggggcaactc aatgccgcat attttcccgg tagctgcgta
     2161 gtccttccag ccagagccag agacgtagcc gcggacaacg cattcgaccg gcagaggctc
     2221 ggtcttgcga acgatcatag agcgtcccgc caactcgtcg cgataagcgt taaaaggagc
     2281 agggaaatga tccgcgctca gcacatgatt gggtatgacg tccttgaaaa gatcgaacca
     2341 gaagagcgat agctgggtca gcacccggcc tttgtcggga atcggcgtcg gcaaaatgac
     2401 gtcgaaagcg gacaggcgat cggtcgcgac gataagtaac ttatcaccga gatcgtaaat
     2461 gtcgcggacc ttgccgcgag cggaaggctc gaggccggca aaatgggttt cacgcaggat
     2521 gcggctcgca ggaatggcag gcaaaaaaac tttcccccga caaagtaaaa acattttagc
     2581 gcacagcgag tcgatgtcaa acgcacaaca gtggaaaact aaggaaagat tagtgacaga
     2641 aaatttcttc ggccaacaaa aaatttatca tctcagagtt tttggtcgta acagcgatgt
     2701 tgcgcgcgag cgaactggtt gagtgagaca aaacaacatt ttgaggaagc cggcaagccg
     2761 tcaggctcgc cggcgctcaa ccagatattt gaaaaaaagt ttaggctgcg gaagcggcgg
     2821 cagcgcgttg tttgggctcg gcatcgcctt cccaacgaac gctgacaagt tttgaaacgc
     2881 cgggttcctg catggtcacg ccgtaaagaa cgctgcccat ctccatggtt ttgcggttat
     2941 gggtgacgat gatgaattgg gtttgatcgc tcatgtcgcc gatgaggcgc gtgaaccgcc
     3001 cgacattggc ttcatccagc ggcgcgtcca cttcgtccag aatacagaac gggctgggct
     3061 ggtagcggaa aatggcaatt agcagcgcca gcgcggtcat agccttttcg ccgccgctca
     3121 gcagcaaaat gttttgcaga cgcttgcctg gaggcgaggc aacaatatcg ataccggcat
     3181 ccccggaact gtctgcttcc gtcaggcgca tttccgccat gcccccgccg aagatcgtgt
     3241 ggaaggcttc ggagaagctt ttgttgatgg cgtggaaagc gtgttcgaac ttttccttgg
     3301 tcgcggcgtc gagttcagcg atggcttgct gggtgtcttg aatggatttc agcaaatcat
     3361 cgcgttcccg ggtgaggaac gtgaagcgct ggtcgcactc gttaaattcc tcgagggcca
     3421 tcatgtttac ggcgcccatg gagtcgatgc gctccttcat ttcgcggtag ctgatttcgg
     3481 cggcggcgag ttcttcgccg ctcatgaaag ccgtctccgt cgcgaggagg tcttcaggct
     3541 gggcgtttac ttccgccatg caagtttcgc gcagatgttg gcggtcggaa tcgttgcgcg
     3601 cgcgagcaat ttcggcgtgg ctgcgttgtt cacgcaactc ttgtagcgac tggcggccca
     3661 tgcgcaggtg atcttccatt tgcgcgacac ggttgcgtcc gccatcccac tcctgttcca
     3721 tttcgtgctg gcgtgtttcg agacgcaatt tctcagcgcg taggccttca agttggagag
     3781 ctaattcctc gctttgctgg gtgaggctgg ctagttcctc agttacggaa ttaagttgct
     3841 ggtgcaggga tgcttcgcga cgcacgagtt cggaatgttc ttcagcaaga cgtgtggcga
     3901 gagcctcggc ggcactgagg cgttcattca tagctgctag ctccgcgcgc ccggccgcca
     3961 attcattctg gtccgattcg atggagccgc gcagttcgct taactgttcg gtgagccgtg
     4021 atctttcggc ctccgcgtga gcgcgatttg cagtagcggt ggcgtgttca tttttggcac
     4081 ggtccgcacg agtgcgcgct tgttccacgt cttgacggat acggcgcaat tcgttctggc
     4141 aaaccgtcag ttcgagaccc agccgagcca gttcgctttg catctgctgg tgacggtgtt
     4201 tggaagcaaa aacattccga tcggcttcac gctgttgctg atcgacgtct tccagagcct
     4261 tctgtgcgag ctgcaaatcg gcggtgagag cttctaaagt agcctgtcgc gctgtggttt
     4321 cgtgctcaag ctgcatcact tcggcatcca gtgcgcgaag ttcgcgtttc atacccagag
     4381 ggccggcttc atcagcgcgt ccgccggtaa cca
//
LOCUS       DATCBB010000187         1167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_168261, whole genome shotgun sequence.
ACCESSION   DATCBB010000187 DATCBB010000000
VERSION     DATCBB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168261"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VII37_02120"
     CDS             <1..750
                     /locus_tag="VII37_02120"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY5068544.1"
                     /translation="PIYLLHRYQVEAATKFIGGMDYTFALRGDGQTPLQIVAPAEQRR
                     ALTAVLATLKPDVLALPESLLNIIPPRVPDYERGREHFKIRTSPAFDALAPAEAAAEH
                     TLQLLFNAERASRLVEFHSRDAKNPGLDEVLDAVLNATWKASRDPGYFGEISRVVDSV
                     ALYDLMALAVSERASAQARAIASLKLEELRNWAANARNGVKDSEERAHLYFAATQIAQ
                     FQKDPELIPVPTPAQPPDGPPIGDDDDAWLP"
     gene            824..>1167
                     /locus_tag="VII37_02125"
     CDS             824..>1167
                     /locus_tag="VII37_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068545.1"
                     /translation="MSLKISVAGLFVAGVLSGPIAASPVSSHHHSISMSGGEHGPVAD
                     CAGLRIHLNDRAAEMRFEERTITRPDGPLRVESEVNGGLQVQGWDKDTYSVTVCKAAD
                     ASMSNVEQIFSQ"
BASE COUNT          251 a          331 c          331 g          254 t
ORIGIN      
        1 cccatctatt tgctgcatcg ctaccaagtg gaagcggcga ccaaattcat tggaggcatg
       61 gactacacct tcgcattgcg tggcgatgga cagactccct tgcagatcgt ggctcccgcg
      121 gaacaacggc gcgcgcttac cgccgtcctt gcgacattga agcccgatgt gctggccttg
      181 ccggagtcac tgctgaacat tattccgcct cgcgtaccag actacgaacg cgggcgtgag
      241 cattttaaga ttcgcaccag tccggcgttt gatgcattgg cgcccgccga ggctgctgct
      301 gaacatacgc tccagctcct tttcaatgcc gagcgcgcat cgcggttggt ggaatttcat
      361 tcgcgcgacg caaaaaatcc aggcttggac gaagtgctgg acgcggttct caatgccacg
      421 tggaaggctt cgcgcgatcc tggatatttt ggggaaatct cacgggtggt agacagtgtt
      481 gctctctacg atttgatggc actcgccgtc agtgagcgag cctcggcaca agcgcgcgcc
      541 attgcttcgc tgaaattaga agagctacga aactgggccg ctaacgcaag gaatggagta
      601 aaagatagcg aagagcgcgc ccacctttat ttcgctgcca cacagatcgc gcaattccaa
      661 aaagaccccg agctgatccc ggttccgacg ccagcgcagc cgcccgacgg gcctccgata
      721 ggagatgacg atgatgcttg gctcccctga ccgttctaag cggctgtccg gctgaacgga
      781 acttttcagc tcagccttcg tctatacagc tggagggatg tgtatgtctc taaaaatctc
      841 tgtggctggt ttatttgttg ccggcgtttt gagcggcccg attgccgcga gtccagtatc
      901 ttcccatcac cattccatca gtatgtccgg aggcgaacac ggcccggtcg ccgactgcgc
      961 cggtcttcgc attcacttga acgaccgcgc agccgaaatg cgttttgaag aacgaaccat
     1021 taccaggccc gacgggccat tgcgcgtcga gtcagaagtg aatggaggac tgcaagtgca
     1081 gggctgggac aaagatactt actcggtgac ggtctgcaaa gcggcggacg cttcaatgag
     1141 taacgtcgag cagatctttt cgcagat
//
LOCUS       DATCBB010000188         3488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_168309, whole genome shotgun sequence.
ACCESSION   DATCBB010000188 DATCBB010000000
VERSION     DATCBB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168309"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VII37_02130"
     CDS             <1..200
                     /locus_tag="VII37_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068546.1"
                     /translation="QETPRETVARCYPLLIAARRKSYETLAHCSVLLSELRGTHSSDK
                     EKNSGDVERFLQKIREQVEHT"
     gene            235..1536
                     /gene="thrC"
                     /locus_tag="VII37_02135"
     CDS             235..1536
                     /gene="thrC"
                     /locus_tag="VII37_02135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5068547.1"
                     /translation="MRYRSTSREAPLTSLRGAVLRGLAPDGGLYMPVEIARRSPEELE
                     EFRRLPFTEVCFRVARPFAGAEVPEEVMWQVVSEAINFPVELVSLSPALHILELFHGP
                     TLAFKDFGARFMARLMAYFVRDETRTLTVLVATSGDTGSAVAHGFLGVPGIRVVILYP
                     SKRISEAQEKQFTTLGENITALEVQGTFDDCQRLVKQAFSDVELNKRAFLTSANSINI
                     GRLLPQMFYHVAAYRQLPVASVPLIVSVPSGNFGNLTAGLFAKRIGLPVTRFVAATNA
                     NDVVPEYLRSGKFAPHPAFATLSNAMDVGNPNNFPRLLDLCRNRLEYVQKEIWGHGAT
                     DAETLAAMKLVYERYQYIADPHTAVGILGWEAYRKAHPEPAQGLVLATAHPAKFAEVV
                     TRAIGIAPRLPARLAAYLKREKLSLPMSNGYDEFKQFLLAH"
     gene            complement(1629..2657)
                     /locus_tag="VII37_02140"
     CDS             complement(1629..2657)
                     /locus_tag="VII37_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY5068548.1"
                     /translation="MALLAALSVAGHKAATAQEGSAAGKSGYRLIKKVKLGGEGGWDY
                     LTVDPASRRLFISRGTHVMVVNADEGKVVGDIPNTQGVHGIALVPDMNKGFTSNGKSN
                     TSTVFDVKTLQPTGEATTEKNPDAIIYDPSSKRVFTFNGGSDSATAIDAATGKTVGTI
                     ALGGGPEFAASDGKGHVFVNLEDKSQLVKFDPVTLKVEKTWPLAPCESPSGLAIDAEH
                     EILVVGCHNKMMAFVDGSSGNVIGTVPICQGVDANRFDSETGFAFASCGDGTVTVAHE
                     DSPDKFSVVDTITTQKGARTMELDTSNHNLYLVSADFGATPAATPEHPRPRPAIVPDT
                     FTLLIYGR"
     gene            complement(2823..>3488)
                     /locus_tag="VII37_02145"
     CDS             complement(2823..>3488)
                     /locus_tag="VII37_02145"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY5068549.1"
                     /translation="HRYFSHRAFKTSRVFQFIIAFMAMSSAQKGVLWWAAHHRHHHRH
                     SDQELDLHSPTLFGFFWSHVGWIMSDKYNDTRLNYIGDFAKFPELRWLNKYHMVPPVA
                     LGAVIWLIGGWHLFIWGFCLSTVLLWHDTFTINSLSHLFGTRRYETTDTSKNNWFLAL
                     LTLGEGWHNNHHHFMASARQGFFWWEVDITYYTLKVLSWFGIVWDLRKVPVHLLSEQK
                     LAA"
BASE COUNT          772 a          920 c         1011 g          785 t
ORIGIN      
        1 atcaggaaac gccgcgcgaa acggtggcgc gctgttaccc gctgttgatc gcggcgcgtc
       61 ggaagagtta tgaaacgctg gcacattgct cggtgctttt gtccgagctg cgcggaacgc
      121 acagcagtga caaggagaaa aattcggggg atgtagaacg gttcctgcaa aagatccgcg
      181 agcaggttga acacacctga tgtttttaac acacaacaaa agtgcagctc tgcgatgaga
      241 taccgcagca catcccggga agcgccactg acgtcgctgc gcggcgcggt gttgcgcggc
      301 ctggcgccgg acggcgggtt gtacatgccg gtggaaatcg cgcgtcgctc tccggaggag
      361 ctggaggaat tccggcggct gcccttcacg gaagtatgtt ttcgcgtggc gcggccgttt
      421 gccggcgcgg aagtgccgga agaagtgatg tggcaagtgg tgagtgaggc gattaatttt
      481 ccggtggagc tggtgtcgct gtcgcccgca ttgcacattc ttgaactgtt tcacgggcca
      541 acactggcgt tcaaagattt tggcgcgcga ttcatggccc ggctcatggc ttatttcgtg
      601 cgtgacgaaa cacgcacgct gaccgtgctg gtggctactt ctggtgatac cggcagcgcc
      661 gtcgcgcatg gttttctggg tgtgccggga attcgcgtgg tgattcttta cccttcgaag
      721 cgcattagcg aagcgcagga aaaacaattt acaacgctgg gagagaacat taccgcgctg
      781 gaggtgcaag gaacctttga cgattgccag cgactggtga agcaggcgtt ttcggatgtg
      841 gaactgaaca agcgcgcgtt tttaacttcg gcgaattcca tcaatatcgg gcggctgttg
      901 ccgcagatgt tttatcacgt ggccgcgtac cggcaattgc cggtggcttc cgtgccgctg
      961 attgtttctg tgcccagcgg gaatttcggc aacttgacgg cggggctttt tgccaagcgc
     1021 atcggattgc cggtgacgag atttgtggct gcgacaaatg ccaacgatgt ggtgccggaa
     1081 tatttgcgca gcgggaaatt tgcgccgcat ccagcgtttg cgaccttgtc gaacgccatg
     1141 gacgtgggga atccgaacaa ttttccgagg ttgctggatt tgtgccgcaa ccggctagag
     1201 tacgtgcaga aagaaatttg gggacacggc gcgacggatg cggagacgct ggcagcgatg
     1261 aagttggtgt atgaacggta tcaatacatt gctgatccgc acacggcggt ggggatttta
     1321 ggttgggagg cgtaccgcaa ggcacacccg gaacccgcgc agggattggt gctggctacc
     1381 gcacatccgg caaaatttgc ggaagtggtc acgcgcgcga ttggtattgc tccacgattg
     1441 ccagccaggc ttgcggcata tctgaagcgg gagaaactgt cgttaccgat gtcgaatgga
     1501 tatgacgagt tcaaacagtt tcttttggca cactgaggcg ctttttatct tccacgaatt
     1561 ttatcgggtc gattttatta gcgcgaattc accttccgaa ttttacctac cgcatttacc
     1621 tgccgcattt acctgccata gatcagcagc gtgaaggtgt ccggcacaat cgcgggacgc
     1681 ggcctggggt gctctggtgt ggccgccggc gtcgctccga aatccgcgct caccaaatat
     1741 aaattgtgat ttgaggtgtc cagttccatg gtgcgcgcgc ctttttgcgt ggtgatggtg
     1801 tccaccaccg aaaatttgtc cggcgaatct tcatgcgcaa cagtaaccgt tccgtcgccg
     1861 cacgacgcga aagcgaaacc tgtctcagag tcgaagcgat tggcatccac gccttgacag
     1921 ataggcaccg tgccgatcac attcccggaa ctgccgtcca cgaaagccat cattttgtta
     1981 tggcagccga cgaccagaat ctcgtgttcg gcgtcaatgg ccaggccgga aggtgattca
     2041 caaggggcca gcggccaggt cttttccacc ttcaatgtga cgggatcgaa ttttacgagc
     2101 tggctcttgt cttccagatt tacgaacaca tgacccttgc catcggaagc cgcgaattca
     2161 ggcccgccgc ccagcgcgat ggtgccaacc gttttgcccg tcgcggcgtc gatggcggtg
     2221 gccgagtcag agccaccgtt aaaagtaaac acacgcttgg acgagggatc ataaataatg
     2281 gcgtccggat ttttttctgt cgtggcttcc ccggtgggct gcaaggtctt cacgtcaaat
     2341 actgttgacg tgttggattt cccgttgctc gtgaatccct tattcatgtc cgggacgagc
     2401 gcgatgccat gcacgccctg cgtattggga atgtcgccga caactttgcc ttcgtcggcg
     2461 ttcactacca tcacgtgggt cccgcgcgaa atgaacaagc gccgactggc cggatccacg
     2521 gtgagataat cccagccgcc ctcgccgcca agcttcactt tttttatcag gcgatatcca
     2581 gacttgcccg ccgcactgcc ttcctgggcc gtagccgcct tgtgaccagc tacactcaac
     2641 gcagcaagca gagccaaaag tcctaaatat gtcagccaag ttttccagta atgtcgcacg
     2701 gttcctcctc aaaaaaatca gacctactat acacgcccca agaacactct tggcactcgg
     2761 aataaaaaaa ggacaggctg gaggcctgtc ccttggtctt cttgtgacgg aaatttaaga
     2821 aattaggcag cgagtttttg ttcgctcagc agatgcacgg ggaccttacg caaatcccac
     2881 acgatgccaa accatgacag cactttcagc gtgtaatagg taatgtcgac ttcccaccag
     2941 aaaaatccct gacgggctga ggccataaag tgatggtgat tgttgtgcca gccttcgccg
     3001 agggtcagca gcgccaaaaa ccaattgttt ttgctggtgt cggtcgtctc atagcggcgc
     3061 gtgccgaaca agtgtgacag cgaattgatg gtgaaggtgt cgtgccacaa cagtaccgtg
     3121 ctcaggcaga agccccagat aaacaagtgc cagccgccaa tgagccaaat caccgcgccc
     3181 agcgctacgg gaggaaccat gtgatattta ttcagccagc gcagttcggg aaatttcgcg
     3241 aaatcgccga tatagttcag gcgcgtgtcg ttgtatttat cggacatgat ccagcccacg
     3301 tgcgaccaga aaaatccaaa tagcgtcggt gaatgaaggt ccagttcctg atcggaatgg
     3361 cggtgatgat ggcgatgatg cgccgcccac cacagcacgc ccttttgcgc tgagctcatg
     3421 gccataaacg cgataataaa ttggaagacg cggctggttt taaacgcgcg atgcgaaaaa
     3481 tagcggtg
//
LOCUS       DATCBB010000189         1618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_265777, whole genome shotgun sequence.
ACCESSION   DATCBB010000189 DATCBB010000000
VERSION     DATCBB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265777"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..1306
                     /locus_tag="VII37_02150"
     CDS             503..1306
                     /locus_tag="VII37_02150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5068550.1"
                     /translation="MGPASRTETMSERSAAPVAVVFAGRRLAARLEPFDSYWQAPADV
                     ESGYSSFSAYYRANYLSHLPADRNARILIVSCGPGYLVNLLAQEGYRNVLGIDSDADK
                     IAHAKRRGLACEVAEAFPFLMERQSPQFDVIIPEQELNHLTVEETIDFLRLCRGALLP
                     GGRVIVYAMNGANPLVGSENIAHNIDHFYNVTEHSLQQILKLGGFTHVSVFPLRLYVF
                     WKNPLNYVGLAVTATFELLLRVVFVLYGKKVRVLSKKIAAVAFERDVVR"
     gene            complement(1315..>1618)
                     /locus_tag="VII37_02155"
     CDS             complement(1315..>1618)
                     /locus_tag="VII37_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068551.1"
                     /translation="GAKTVTTNAMVSAISAALGSSRRIFHAPMWPFVAAAYVLESTMT
                     PLGLKPPLHRRRLDFFRKSFRFSTAKAQKLLGFRADTDFIEGARRTAEWYRANNYL"
BASE COUNT          318 a          466 c          480 g          354 t
ORIGIN      
        1 gcccgttcca gtccgtcttc tacggacgga tcttgctccc tagccattga gtctcctgca
       61 actgcagcaa agccatcagc acgcgaactc atctcgggaa gccaataatt gcgcggcaga
      121 tcccgataca gacggggtgc cgctcacaat atcgggcaac cgcagggtag cgcattgtga
      181 agaattgaaa gcctagctcg gccgtaacgc gcttgccgcg acgcggttcc gcatcacctt
      241 caggtcgcgc ataaacgcgc ctggcgggga gggccgaagg gcacaatgag tcggcgcgga
      301 ggcctgctgt ccacagctcg ctgcgcgacc gtgggaacgt aggctctctg ggcgtaccgc
      361 ggccaagctt gtaggggccc gcagcgccag cgcggtgcat gtgacattat atcggtgggt
      421 cgcgcttcgc tggccgtggt ccgcgcatcc tctatactcg cggggccgaa taggaggccg
      481 catgtctgga agtccgccgc ttttgggtcc tgcttctcgc accgagacga tgtcggagcg
      541 cagcgccgcc ccagtggctg tcgttttcgc cggtcgcagg cttgccgcgc ggctcgaacc
      601 tttcgactcc tattggcaag cacccgcaga tgtcgagagc gggtacagct ccttcagcgc
      661 ttactatcgt gcaaattacc tctcgcacct accagcggat cgcaacgcgc gcatcctcat
      721 cgtcagttgc ggtcccgggt atttggtaaa tctcctggcg caggagggct accgcaatgt
      781 gctcggcatt gattctgacg cagacaagat tgctcacgct aagcgtagag gcttggcttg
      841 cgaggtagcc gaggcatttc ctttccttat ggagcgtcag tccccgcaat ttgacgtgat
      901 catccccgag caggagctga atcatcttac ggtcgaggaa acaattgatt ttctgaggtt
      961 atgccgcggc gcacttctgc cgggcggccg ggtaatcgtg tatgcaatga atggcgcaaa
     1021 tccgctcgtc ggttcggaga acattgcgca caacattgat catttttata atgtgaccga
     1081 gcatagtctg cagcagatcc tcaagctcgg gggcttcacg cacgtcagcg ttttcccgct
     1141 taggctgtac gtcttttgga aaaatccgct caactatgtc gggctcgcgg tcaccgcgac
     1201 atttgagctg ctgctgcgcg tggtattcgt gctttacggc aagaaggtgc gcgtgctgag
     1261 caaaaagatt gctgcggtcg cattcgagcg tgacgttgtg cgctgagccg atcgtcaaag
     1321 ataattgttt gcccgatacc attctgccgt ccgacgcgcg ccctctatga agtctgtgtc
     1381 agcgcggaac ccgagtaatt tctgtgcttt ggcggtggaa aacctgaagg atttgcgaaa
     1441 gaaatccagc cggcgccgat gcagcggggg cttcaatcct aatggcgtca tggtggattc
     1501 caatacataa gcagcggcca caaacggcca catgggcgcg tgaaaaatgc gccgactact
     1561 accgagcgcg gcgctaatcg cgctaaccat tgcgttcgtt gtgacagtct tggcgccg
//
LOCUS       DATCBB010000190         1551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_136046, whole genome shotgun sequence.
ACCESSION   DATCBB010000190 DATCBB010000000
VERSION     DATCBB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136046"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VII37_02160"
     CDS             <1..540
                     /locus_tag="VII37_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5068552.1"
                     /translation="AILLDVRLPGISGIEVCRELRQRGIESPVMMLTARTLVEQRVEG
                     LDAGADDYLTKPFVLAELLARVRALVRRGFHKGNARLHYADLELDRHRRRAMRGKEII
                     PLTSKEFALLELFLLHAPDLITRSAIVEHVWDYHFDSETNLVEVYINRLRQKIDVSGA
                     PKLIHTVRGVGYRLGMPES"
     gene            complement(599..1012)
                     /locus_tag="VII37_02165"
     CDS             complement(599..1012)
                     /locus_tag="VII37_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5068553.1"
                     /translation="MKILTSEMRGCRKGPQDWFTGTVWIDEIVAAAAPARFAVNRVSF
                     EPGTRTAWHTHPMGQALHVLTGAGRVQLAGKGPQIITAGDSVWIEAGERHWHGADASH
                     TMVHLAIQETDQNGVNVVWLEHVSDEEYALPVERS"
     gene            complement(1059..>1551)
                     /locus_tag="VII37_02170"
     CDS             complement(1059..>1551)
                     /locus_tag="VII37_02170"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY5068554.1"
                     /translation="PDGTISGDYEKYCAGYEAAIQSAGGIDLQILGIGRNGHIGFNEP
                     TSSFASKTRLKVLSKATVEDNRKFFGAGETVPRLAITMGIGTILASRRVLMLATGAAK
                     AGAVANAIEGPITASVTASALQLHADATAIVDEEAATQLKQKDYYRDVMEMTALFTPG
                     RLS"
BASE COUNT          335 a          508 c          357 g          351 t
ORIGIN      
        1 gctatccttc tggatgtgcg cctaccaggc ataagcggaa tcgaagtctg ccgcgaattg
       61 cgccagcgcg gaatcgaatc tccggtaatg atgctcactg cgcgaactct cgtcgagcaa
      121 cgcgtcgagg gcctggatgc cggcgccgac gattatttaa ccaagccctt cgtgctcgcc
      181 gaattactcg cgcgcgtgag agctctcgtt cgccgcggct tccacaaagg taatgcccga
      241 ctgcactatg ctgatttaga actcgatcgt catcgccgcc gcgccatgcg tggtaaggaa
      301 ataattcccc taacctccaa ggaatttgcg cttctggagc ttttcttgtt gcacgctcca
      361 gacttgatca cccgcagcgc cattgtcgaa cacgtgtggg actaccattt cgacagcgag
      421 accaatctcg tcgaggtata tatcaatcgc ttgcgccaga aaatcgacgt cagcggcgcg
      481 ccgaaattaa tccacaccgt gcgaggcgtc ggctaccgct taggcatgcc cgaaagctag
      541 acccgttcta ccctcactaa gaaaacttca aatcccgcag caacaagcct aagtagcatc
      601 aagaacgttc gaccggcaat gcatactcct cgtcgctcac atgttccagc cacaccacgt
      661 taactccatt ttggtccgtt tcttgaatcg ccagatgaac catcgtgtgg ctcgcatcag
      721 ctccatgcca gtgccgttct ccggcctcaa tccaaacaga atcgcccgcc gtgataattt
      781 gcggcccctt accagccagt tgcacccggc ccgctcccgt taacacatgc aaagcctgcc
      841 ccatggggtg tgtatgccac gcggttcgcg tcccgggctc gaacgaaacg cgattcaccg
      901 caaatctcgc aggcgcggca gcggccacga tctcgtcaat ccacaccgtc cccgtaaacc
      961 aatcctgcgg ccctttgcgg caaccacgca tctcgctcgt aagtattttc atttttcaat
     1021 ccccaccact tgtcctcgtc attccccatc acgcggaatt acgacaacct tcccggcgta
     1081 aacagcgccg tcatctccat cacatcccga taataatctt tttgcttcaa ttgcgtggcc
     1141 gcttcttcat caactattgc ggtggcgtcc gcatgaagtt gtaatgctga cgccgtgacc
     1201 gatgcggtga taggtccctc gatagcattc gcaacagccc cagctttcgc cgcaccggta
     1261 gccaacataa gcacgcgcct gcttgccagt atcgtgccga tccccatagt gatcgcgagg
     1321 cgcggcacgg tttccccagc gccaaagaat ttccgattgt cctctacggt cgctttgcta
     1381 agcaccttca ggcgcgtttt cgacgcaaaa ctagaagtcg gttcgttgaa cccgatgtgc
     1441 ccattgcgcc cgatgcccag gatttgcaga tcaatccctc cagcactttg aattgcagct
     1501 tcatacccag cgcaatattt ttcatagtcg ccgctgatcg tcccatccgg g
//
LOCUS       DATCBB010000191         1013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_232947, whole genome shotgun sequence.
ACCESSION   DATCBB010000191 DATCBB010000000
VERSION     DATCBB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232947"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..>1013
                     /locus_tag="VII37_02175"
     CDS             52..>1013
                     /locus_tag="VII37_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5068555.1"
                     /translation="MAPFWHFNQASYDSRANDFPVATTWHQKSDYAGAQADVRADVGA
                     NNFSGGMYSFYQGENDLFGLAINDKSFAANSVPNTAATSRAGLVEFHVSDHLRVGEYI
                     TLMGGMRFSIYRAGFNESAAYPRIGATVRIPRLNWVLRGFYGHFFQPAPVETVSNSFL
                     NYVTSQQGQNAFVPLPSERNEEHQFGIAIPYRGWMLDVDTFKSRVNNFLDHANVGESN
                     VYFPIAVDGALVRAWEMTLRSPSLARLGQFHLTYSNQIAEQRGNVIGGFACSDPADPA
                     CNMGFDYTPVDHDQRNALNAGFTANLPAHTWFATNVYYGSGFTNG"
BASE COUNT          203 a          246 c          327 g          237 t
ORIGIN      
        1 tttgcgattc ttgattggac gcatacggtc tcagcgaagg ctgagttttc agtggcgccg
       61 ttctggcact tcaaccaggc gagctacgat tcacgcgcaa acgacttccc ggtggcgact
      121 acgtggcacc agaaatcgga ctacgcgggt gcgcaggcgg atgtgcgcgc agatgtaggg
      181 gcgaacaatt tctccggcgg gatgtacagc ttttaccagg gcgagaacga tttgtttgga
      241 ctggcgatca acgacaagag ctttgccgct aacagcgttc ccaacactgc ggctacatcg
      301 agggcggggc tggttgagtt tcatgtttcg gatcatctgc gcgtgggtga atacattacg
      361 ctgatgggcg ggatgcggtt ttcgatttat cgagcgggat tcaacgagag cgcggcttat
      421 ccgcgaattg gggcgacggt gaggattccg cggttgaact gggttttgcg cggattttac
      481 ggacactttt ttcagcctgc gcccgtcgag acggtttcga attcttttct gaattacgtg
      541 accagccagc aggggcagaa tgcgtttgtg ccgctgcctt cagaacgcaa tgaggagcac
      601 cagttcggga ttgcgattcc gtaccgggga tggatgctgg atgtggacac gttcaagagc
      661 cgcgtgaaca attttctgga tcacgcaaat gttggcgagt cgaatgtcta tttcccgatc
      721 gccgtggatg gagcgctggt gagggcatgg gagatgacgc tgcgttcgcc aagcctggcg
      781 cggctggggc agtttcacct gacttactcg aaccagatcg cggagcagcg cggcaatgtg
      841 atcggcgggt ttgcgtgcag tgatcctgca gacccggcat gcaacatggg gttcgactac
      901 actccggtgg atcacgatca acgcaatgcg ctgaacgcgg ggttcacggc gaatctgccg
      961 gcacacacgt ggtttgctac caatgtttac tacggatcgg gttttacgaa tgg
//
LOCUS       DATCBB010000192         1599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_265866, whole genome shotgun sequence.
ACCESSION   DATCBB010000192 DATCBB010000000
VERSION     DATCBB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265866"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VII37_02180"
     CDS             complement(<1..232)
                     /locus_tag="VII37_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068556.1"
                     /translation="MLTAAFFAVKMNQASALPLQSIGTATYELLVPVACKYGSGKCAD
                     VKPQENLPSTKKLGPEDPTVDPDCAHYGNCRGG"
     gene            complement(356..928)
                     /locus_tag="VII37_02185"
     CDS             complement(356..928)
                     /locus_tag="VII37_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068557.1"
                     /translation="MRKFRKIQLLGVGILTPSLVGQPQIAEARSADFTEPLKLEAANA
                     STATLVEMFKRELFALTPAEKISIAGDRIRLSAQQLNSKKAPVVSGDEHGITYQDGCG
                     IPSPKVNKQNPNRQNLRVSVDGDGCTANCAGSRLKMNNNLPLNRQNLRVSVDGDGCTA
                     NCAGSRVKMNNNLPTANCAGSRMKSGTQIK"
     gene            1099..>1599
                     /locus_tag="VII37_02190"
     CDS             1099..>1599
                     /locus_tag="VII37_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068558.1"
                     /translation="MTRWQAGMTLAERLAARGQQQLRHRTDPPNAAGQRKRWRAERRA
                     DIARASEPAAIRLDPPLWLRTFQAAYARAEEPKLPINQADDFLLAVEPVIAMARDRID
                     AALRGPISSMAPAAREDRAALVDSLELGLRRRLFDVISKTLVLELAVASRRKILAGNS
                     SEDRFAF"
BASE COUNT          336 a          462 c          421 g          380 t
ORIGIN      
        1 tacccccacg gcagttgcca taatgtgcgc agtcgggatc gaccgtggga tcctcaggcc
       61 ctagcttttt ggtcgatggc aaattttcct ggggcttaac atccgcacat ttgccactgc
      121 cgtatttaca ggcgaccggg actaagagct cataagttgc ggtgccaatg gattgcaacg
      181 gcaacgcgga ggcctgattc attttcactg caaagaaagc ggcggtgagc atgaatgcgt
      241 acgcgcttct cgataccaat ttcatacatc ctcccaattt tatgggtttc aattcgtgtt
      301 ttgaggctcc tagctatcgc cgtccaacaa cttggctaga ctcgatcgcc gattatcatt
      361 ttatttgcgt acccgacttc atacggctcc ctgcgcaatt cgccgtcgga agattattgt
      421 tcatcttcac acggcttcct gcgcaattag ccgtacatcc atcgccatcg acggacaccc
      481 tcagattctg cctgttcaat gggagattat tgttcatctt cagacggctt cctgcgcaat
      541 tcgccgtaca gccatcacca tcgacagaaa ccctcagatt ctgcctgttc ggattctgct
      601 tgttcacctt cggcgaaggt atgccgcagc cgtcctgata agtgatcccg tgttcatcgc
      661 cgcttacgac aggtgccttt tttgaattaa gctgctgggc gctcaggcgg atgcggtcac
      721 cggcgatgct gatcttttcc gcgggggtaa gcgcgaaaag ctcccgtttg aacatttcca
      781 ccagcgttgc agtcgaggca ttcgcagcct ccagcttcag tggctcggtg aaatcggcgg
      841 agcgagcctc cgcgatctgc ggctgaccca ccagcgaagg ggtcaaaata ccaacgccca
      901 ataattgaat tttccgaaat tttctcatgt agacctctcc gagaaaatcg acctctttga
      961 agaaaacatt ctagaatgac acaataatac tgataacatc gacctgtggt gtcaatcctt
     1021 tacggaacgg cgcacgctat atcagtacga ccctggttga tttgtctgat cgatgacgct
     1081 gaaccctgca gccgcgcgat gacccgttgg caagccggca tgacgttggc ggagcgcctc
     1141 gccgcaagag gccagcaaca gcttcgccat cgaacagatc cgccaaatgc tgcgggccaa
     1201 aggaaacggt ggcgcgcgga gcggcgtgcc gatattgccc gagccagcga acctgccgcc
     1261 atccggcttg atccaccctt gtggctgcgg acctttcagg ccgcctacgc cagggcggag
     1321 gagccgaagc tgccgatcaa ccaggccgac gactttctgc tggctgttga acctgtcatc
     1381 gcgatggcgc gcgaccgcat cgatgcggcg ttgcgaggac ctatttcaag catggctcct
     1441 gcggccaggg aagatcgcgc cgcgttggtt gattcgcttg aacttggcct tcgcaggcgg
     1501 ctgttcgatg ttatttcaaa aacgctggtt cttgaactgg cggtcgccag ccggcgcaag
     1561 attctcgccg gaaacagctc tgaggatcgt ttcgcgttt
//
LOCUS       DATCBB010000193         1154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_460698, whole genome shotgun sequence.
ACCESSION   DATCBB010000193 DATCBB010000000
VERSION     DATCBB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460698"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VII37_02195"
     CDS             complement(<1..201)
                     /locus_tag="VII37_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068559.1"
                     /translation="MGKLRGVSAAACNFACVSAVLLAWTLSAAAQGGIPSPTATATAA
                     NDPITNPEYNALFKRMFANPTDL"
     gene            complement(672..914)
                     /locus_tag="VII37_02200"
     CDS             complement(672..914)
                     /locus_tag="VII37_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068560.1"
                     /translation="MTVKFVRERPHVGGDKHDQNWGTDMGKLANTLLAWLIAIMFVAA
                     PVAALARPKDNPNYGFCKSGKQVTDIKKCKENGGKL"
BASE COUNT          254 a          335 c          289 g          276 t
ORIGIN      
        1 gagatcagtc ggattggcga acatccgctt aaacagcgcg ttgtattccg gattggtgat
       61 ggggtcgttc gctgccgtcg ctgtggcagt cggggacggg attccacctt gtgccgccgc
      121 acttaaggtc cacgcgagca ataccgcgct cacgcatgcg aaattgcatg cagcagcact
      181 taccccccgc aacttaccca cgataccccc cgtcgcaccc taatacccta ataccaaaac
      241 ttagcatttt gcagccggct ggcgagtcaa aaatggtgca caaccggcct cagacggcag
      301 gctgtcgcgg gcctcactct gatctgggat gcttttccga acgtttccag atgcatcagg
      361 gcacacaagc ggtttggcaa ttattcctgt ataatccagg cgccaattcc ttttgagtgc
      421 acgattccaa gcagcgattt tggcaaaagt cattagccgt ttgcgcaaaa gcggagatag
      481 gtctgagtac ttgaagaagt gcaacggcta ttatgagtgc tctcagcaca ttttttgtcc
      541 ccatgcgcgc gttgatgcaa aaactttcgc gccatttcag tcgataggtc aaacgcccgc
      601 acctgccgcc tcgcgcagcg gtctctccga aatcagatta ggtgcgctgc gtggtgacgc
      661 ctgatggcag cttacagttt gccgccattt tccttgcact tcttaatatc ggtaacttgc
      721 ttgccgcttt tacaaaatcc gtaatttgga ttgtccttag gcctagccaa agctgccacc
      781 ggtgcggcaa caaacattat agcgatgagc caagccaaaa gcgtatttgc aagcttcccc
      841 atatccgtcc cccaattttg atcatgttta tcgcccccca catgcggcct ttcacggacg
      901 aatttaaccg tcaattaaaa gttgcaccgt tgtttacgcg atcattgtgc gattagagcc
      961 tactcgaagg cattgcatcg gggctggctc ctttttcaac tcacccacgt atcgtgggag
     1021 ctgcataggg gctaccgcgg tgcagctatt ccgctccgca acgtcgctca tgggtgtgcg
     1081 ccgtgaaaag gcggcgcttt ccagtgggtg cgagccccac ccggcaaccg ctccagccgg
     1141 aagcaatcgg agca
//
LOCUS       DATCBB010000194         1001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_38904, whole genome shotgun sequence.
ACCESSION   DATCBB010000194 DATCBB010000000
VERSION     DATCBB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38904"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VII37_02205"
     CDS             complement(<1..982)
                     /locus_tag="VII37_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068561.1"
                     /translation="MSRWMEALLPLFEEKLSWPSCATSWAQFAAGTVRDLQDKEATVA
                     EAAGHRGLRAYVKESSQIWKQSGDNFELMTLADVLWPSLLYLRLHPSPSFEVECKDLL
                     SDLPGFYNPATRSISNDFKRPADERADSWYPFENGLIKYPMIGSLSGSKEITGHFLEA
                     FGTAQKMAQQYNYLFPIYYQVGTLRAEGAGTNYAVGGLYAWAAILAHKLTGDGHYLEE
                     ARRAVRVLYTVPADRLFHEPQELAYGALAAAELGMHTECKYLLYEQLRMFYWFSDPSQ
                     KSHEIRGMVQAAASILYPAFKENVEAILPWTGIMKRGIVFDGLLRFMDQQR"
BASE COUNT          222 a          270 c          286 g          223 t
ORIGIN      
        1 ggcgttgttg atccatgaaa cgtaacagcc cgtcgaacac gatgccgcgc ttcataattc
       61 cggtccacgg taggatggct tcgacgtttt ctttgaacgc ggggtagaga atggacgccg
      121 cggcttgaac cattccgcga atctcgtgcg atttttggct gggatcagaa aaccaataaa
      181 acattcgcag ctgctcatag agcagatact tgcactccgt atgcatgcca agttctgcgg
      241 cagctagcgc accataggca agttcctgcg gctcatgaaa gagtctgtcc gcgggcacgg
      301 tgtacaaaac gcgaacggcg cggcgtgcct cttccaggta atgaccgtca ccggttaatt
      361 tgtgcgccag aattgcggcc cacgcgtaca agccgccgac ggcgtagttt gtgccagctc
      421 cttcggcgcg aagagtcccg acctggtaat aaatcgggaa gagatagttg tattgctgag
      481 ccattttttg cgcggtgccg aaggcctcga gaaaatgacc ggtaatttcc ttcgagcctg
      541 atagcgaacc gatcatcgga tacttgatga gcccgttttc gaacgggtac caggagtcgg
      601 ctctctcgtc ggccgggcgc ttgaaatcat tgctgatgct gcgagtggcg ggattataga
      661 aaccggggag atcgcttagc agatccttgc attccacttc gaaacttgga gatggatgaa
      721 gccgcaagta aagcagcgag ggccacagaa cgtccgcgag agtcatcaat tcaaagttgt
      781 cgccggattg cttccagatc tgcgagcttt ccttgacgta agcgcgcaat ccgcgatggc
      841 cagcggcttc ggcgaccgtg gcttctttat cttgcaaatc gcggacggtg ccggcggcaa
      901 actgcgccca gctagtcgcg cagctcggcc aggaaagttt ttcttcaaaa agcggcagaa
      961 gcgcttccat ccagcggctc accccttcct gctctgtgat g
//
LOCUS       DATCBB010000195         1428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_460896, whole genome shotgun sequence.
ACCESSION   DATCBB010000195 DATCBB010000000
VERSION     DATCBB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460896"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VII37_02210"
     CDS             complement(<1..917)
                     /locus_tag="VII37_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5068562.1"
                     /translation="MNPRSVIRWLSIFFGIFLCAVTPFNGPQAQAQNAPPQNNQGAGA
                     APVSGEASAKHAAEEAKGTGQRTAGQKLAEEQFKNIQALKGIPADQLIPAMQFITASL
                     GVDCEFCHVHEKMDSDDKKPKKIARQMMTMMLEIDKNNFDGRLEVTCYTCHRGAAMPV
                     SIPVIKDEEMAGPGAAGKKPAENTALPKPEELLDKYLAAVGGAAAVEKITSRVQKGKL
                     MAFGGQNFPADVYSKAPDKRISIMHLQGGDSVTAFDGQRGWLSVPGRPAHMMSASEND
                     SARLDADLHFPVHVKTLYPKFKVEGGEKI"
     gene            complement(1023..>1428)
                     /locus_tag="VII37_02215"
     CDS             complement(1023..>1428)
                     /locus_tag="VII37_02215"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5068563.1"
                     /translation="NSFSLDADLKDAWGLPALRMTYKDHPDDLKVAKWLNDRALELLD
                     AAGATKKWSYPIVEQEFAVHLLGTCRMGDDPKTSVINSDHRTHDVKNLFLCDGSSFVT
                     SGRGQPTMTIEALAFRAADRITALAKSGELNA"
BASE COUNT          282 a          430 c          358 g          358 t
ORIGIN      
        1 tcgatttttt cgccgccctc aaccttaaat ttcgggtaca gcgtttttac gtgcacggga
       61 aaatgcagat cggcatccag gcgcgccgaa tcattttccg acgcagacat catgtgcgct
      121 ggtcttcccg gaacactcaa ccacccgcgc tgtccatcaa atgcggtcac gctgtcgccg
      181 ccttgcagat gcatgataga gatgcgcttg tccggcgctt tcgaatacac atccgccgga
      241 aaattctgcc cgccgaaggc catcagcttt cccttttgta cccggctggt aattttctcg
      301 accgctgccg ctccgccaac cgccgcaaga tatttatcca gcaactcctc gggcttcggc
      361 aaagccgtgt tttccgcggg cttctttcct gccgctccag gaccagccat ttcctcatcc
      421 ttgatcaccg gaatgctcac cggcatagct gcaccccggt ggcacgtgta gcaggtgact
      481 tccagccgtc catcgaaatt gtttttatcg atttctaaca tcatagtcat catctggcgc
      541 gcaatttttt tcggtttttt gtcgtcgctg tccatctttt cgtgcacgtg gcaaaactcg
      601 cagtccacgc ccagggacgc ggtgatgaac tgcatcgcag gaatcagctg gtccgcggga
      661 atgcccttaa gcgcctgaat attcttgaat tgctcttccg ccagcttttg gccggcggtt
      721 ctttgacctg tgccttttgc ctcttcggcg gcgtgcttcg cactggcttc gccactaacc
      781 ggagccgctc cggcgccttg attgttttgt ggaggagcgt tttgtgcctg cgcttgcggg
      841 ccgttgaatg gcgtaaccgc acacagaaaa atcccaaaaa aaatgctcag ccagcgaatc
      901 acgcttcgcg gattcatatt tactcctcgc ctcacgcagg tccacacgcg cagaccccgc
      961 agcacgcaga attgtagcaa agcacctgca ggaagacgct gattttgctt ttaggaaagt
     1021 aattacgcgt tgagctcgcc gctctttgcc agtgcggtaa tgcggtcagc ggcgcgaaac
     1081 gccaacgcct cgatggtcat ggtcggttgg ccgcgaccgg aagttacaaa actgctgcca
     1141 tcgcacaaaa acagattttt tacgtcatgc gttcgatgat cagaattgat caccgatgtt
     1201 tttgggtcgt cgcccatgcg gcaggtgccc agcaaatgca cggcgaattc ctgctccacg
     1261 atcgggtagc tccatttttt tgtggcgccg gcggcgtcca gcaattcgag cgcgcgatcg
     1321 ttcagccact tcgccacttt cagatcgtcg ggatgatctt tataggtcat gcgcaaagcc
     1381 ggcaaccccc acgcatcttt cagatctgcg tcgagcgaaa agctgttg
//
LOCUS       DATCBB010000196         1299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_201017, whole genome shotgun sequence.
ACCESSION   DATCBB010000196 DATCBB010000000
VERSION     DATCBB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201017"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..>1299)
                     /locus_tag="VII37_02220"
     CDS             complement(49..>1299)
                     /locus_tag="VII37_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068564.1"
                     /translation="ASVARVAGLRGLQALLILLGAAIMLALYSYATLRSKNSKGSFVA
                     VSVLLALACANFNLRPQMLGYLFLVLTLIALERFRQGKRAALWFLPPLFLLWINAHGS
                     WEIGLAVILAYWLCGLKEFQLGDIAVRAWKPEERTRLSLIFLLCLAAIPITPYGTRLA
                     AYPFQVASTLPLNLANIEEWRPIALNSSGPQLFLFLVLGTIFAQIAWPLRWRLEDVLL
                     FVGAAGMTFVHARFLLLFVPFFSPVFATMLSRWVPKYQREKDHYVLNVLLMVLMLGGM
                     IHYFPSKAQIEKRIDAAFPAGAVRYLTDHPVAGPMYNSYDFGGYLVYAQHDAHKVFID
                     GRGELYEHGGVFADYVQVAELQPGVLGILNNYGIRFCMLRTGDPLAVFLAARPDWKEV
                     YGDGVSLIVVRNESAARPQSFRER"
BASE COUNT          345 a          400 c          322 g          232 t
ORIGIN      
        1 gaaagcagaa ccgggccgtg caattgacgc gtcatatgca ctcccgcctc atcgctcgcg
       61 aaaactctgg ggtcgcgctg cgctttcatt gcgaacgaca attaaggaca cgccatcgcc
      121 ataaacttct ttccagtccg gacgcgccgc taaaaaaact gccaacggat cgccggttct
      181 caacatgcag aaccgtattc cgtagttgtt caagattccc agtactcccg gctgcagctc
      241 cgcaacctgt acgtaatctg cgaacactcc gccgtgttcg taaagctctc cgcgcccatc
      301 gatgaacact ttatgcgcat cgtgctgcgc atacacaaga taacccccaa aatcgtacga
      361 attgtacatc ggcccggcaa ccggatgatc cgtcaaatag cggaccgcac ccgccggaaa
      421 ggccgcgtca attctctttt caatctgcgc cttcgaggga aagtaatgaa tcatgccgcc
      481 cagcatcaac accatcagta aaacgttcag tacgtaatga tctttttctc gttgatattt
      541 cggcacccac cggctcagca tggtagcaaa gactggcgaa aaaaaaggca cgaaaagcag
      601 caagaagcgc gcgtgcacga aggtcattcc cgcggcgccc acgaacagca gcacatcctc
      661 caagcgccat cgcaacggcc aggcgatctg cgcaaaaatg gtcccgagca ccagaaacaa
      721 aaacagttgc ggcccagagg aattcaacgc gataggccgc cactcctcga tattagccag
      781 attcagcggc aaggtagaag ctacctggaa agggtaggct gccagccgcg tgccgtacgg
      841 cgtgatggga atcgccgcca aacacagcag gaagattagc gagagtcgcg tgcgttcctc
      901 gggtttccag gcacggacgg cgatatcgcc gagctgaaat tcttttaaac cacacaacca
      961 gtacgccagt atgaccgcca atcctatttc ccatgatccg tgcgcattga tccacagcag
     1021 aaacagcggt ggaagaaacc atagggccgc tctcttgcct tggcggaatc gctcgagcgc
     1081 gatcagtgtc agcaccagaa acagatagcc cagcatttgc ggccgcaaat taaagttcgc
     1141 gcacgccaac gccagcagca cgctgacggc cacgaaacta cccttcgaat tcttcgaccg
     1201 caaggttgcg tagctataaa gcgccagcat gatggccgcg cctagaagaa tcagcaaagc
     1261 ctgcaggccg cgcagtccgg caactcgagc cacgctggc
//
LOCUS       DATCBB010000197         1287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_168605, whole genome shotgun sequence.
ACCESSION   DATCBB010000197 DATCBB010000000
VERSION     DATCBB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168605"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VII37_02225"
     CDS             <1..1049
                     /locus_tag="VII37_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068565.1"
                     /translation="SFGTWTANGKYFLFSGCEQYDCNLWAMRDVWSWLRRSNHTPVLL
                     TSGPDALNVATPAQTGSRIFAFSFRSRRELEKLDPQTMHSTVLSLDTHTEEASVSSDG
                     EMVVYSDRPDGSLWRSRTDGSQRLRLTSPPLSALAPQWSPKGELILFTGVRPGQARQI
                     YIVAADGGALRPVLPEGWEGTHADWSPDGYRIAVVMRNRKTHPQFALFTLEPTTAVWK
                     ELPNSANLNDPRWSPDGRYIAAVDSTNRDLVLFDVQKSRWTSAASGRFLERPYWSADS
                     SYIYFQDQLDDEESVFRVNVGARQVERVAGFGEILRASAAHCYFSGLGRDGSLYMMFE
                     RGLTDIYSLDLDLP"
BASE COUNT          260 a          364 c          377 g          286 t
ORIGIN      
        1 aatcctttgg aacctggaca gccaacggca aatatttcct tttttccggc tgcgaacaat
       61 acgattgtaa tttgtgggct atgcgcgatg tatggagctg gctgagacgc tcgaaccata
      121 cgccggtgtt gttaaccagc ggaccggacg cgttgaacgt ggccaccccg gcgcagaccg
      181 ggtcgcgcat ctttgcgttc agcttccgct cgcggcgcga actcgaaaaa ctcgatccgc
      241 aaaccatgca ctctaccgta ctgagcctgg atacccacac tgaggaggcc agcgtctcgt
      301 ccgacgggga gatggtggtc tattcggacc gacccgatgg ttctttgtgg cgaagccgca
      361 cggatggttc gcaacgtttg cggctgacca gtccgccgct cagcgcactc gcgccgcaat
      421 ggtctcccaa aggtgaactg attcttttta ccggagtgcg tccgggacaa gctcgccaga
      481 tatatattgt tgcggcggac ggaggcgcat tacggcccgt gcttccggaa ggatgggagg
      541 gcacgcatgc ggactggtca cccgatgggt atcgcatcgc cgttgtgatg cgcaaccgca
      601 agactcatcc gcaatttgcg ctctttacgc tcgagccgac taccgcggtg tggaaagaac
      661 tccctaattc ggcgaatctg aatgatccgc ggtggtcgcc cgatgggcgc tacatcgccg
      721 cagtagacag caccaatcgc gacctggttt tgttcgatgt gcaaaagagc agatggactt
      781 cagcagcttc cggccgattc ctggagcggc cgtactggtc ggcggacagt tcgtacattt
      841 attttcaaga ccagctggat gacgaggagt ccgtgtttcg tgtaaatgtt ggcgcgcgac
      901 aggtcgagcg cgttgccgga ttcggagaga tccttcgcgc tagcgccgcg cattgttatt
      961 tcagtggact gggccgggac gggtctctct atatgatgtt tgagcgcgga cttactgaca
     1021 tctattcgct tgatctcgat ctgccctgac tcgcagcctg attcgcaacc tgcctggcga
     1081 cgaagcgcgg cggcttccgc gggaaaactt ctactcttcg ttgaattgat tccgcttttt
     1141 gtaagccatc acagcaatcc cgatgcagat cagcatgggt ggcgcgacca atataaggat
     1201 accgctgcgc aacgcctgca ccaatcgatc gctcgatccg gcggcctgag tatagcaaag
     1261 ggcgcaactc tgcgccgaca cgatgga
//
LOCUS       DATCBB010000198         1006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_266064, whole genome shotgun sequence.
ACCESSION   DATCBB010000198 DATCBB010000000
VERSION     DATCBB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266064"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..588)
                     /locus_tag="VII37_02230"
     CDS             complement(256..588)
                     /locus_tag="VII37_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068566.1"
                     /translation="MSKLCLTLAPAIFLPAELRAQGDDQKPRPRNRPDSKSDEDSPLP
                     AGSRKAVLEENEKDIKTKVEKLFQLASELKEEVEKTDSAKVLSLAMLKKAEEIEKLAK
                     DIKTRAKG"
     gene            608..>1006
                     /locus_tag="VII37_02235"
     CDS             608..>1006
                     /locus_tag="VII37_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068567.1"
                     /translation="MNTAIIPTPNGASAGTELRHGNSSRNCADRFPRSSNVILAAPIK
                     FMPYSLTHWSEFTLRNGLHLLGIVAAALLLNRLLRVVTNLVIKRASSPTRAAQMREQQ
                     TRTLAGLLFSAGTKVVWGIALLTALPEFGIN"
BASE COUNT          189 a          299 c          278 g          240 t
ORIGIN      
        1 ccggtgaggg tcgaactcac gacctttagc ttcggaggct aacgctctat ccatctgagc
       61 tacgggcgcg cggcagagaa atcgttcggt gaattccatt tagccgggcg ccatggcggc
      121 caccggaaac gaaagaataa ccgaacatcg ctattctaac ctctagcgcg atgacgcgct
      181 acaaacgcat cctccatgcg ggcccggcgg catcccccgt gacatcagga ggaatggagc
      241 gatcatttca agtgctcatc cttttgctcg cgtttttatg tctttcgcca atttttcgat
      301 ttcttccgct tttttcaaca tggcgagcga cagcacttta gcggagtcgg tcttttcaac
      361 ttcttctttg agctcgctgg ccaactgaaa cagtttctcg actttggtct tgatgtcttt
      421 ctcgttctct tccagaacgg ctttgcggga gcctgctggc agggggctgt cttcgtcgga
      481 tttcgagtcg ggtctgttcc tgggtctggg tttctggtcg tcgccttgcg cgcgcaactc
      541 tgccggcagg aaaattgccg gcgcaagtgt caggcacagt ttactcaaga actgacgccg
      601 ctgtttcatg aacaccgcta tcatcccaac ccccaatggc gcgagcgccg gcacggagct
      661 tcgtcacgga aattcgtcac ggaactgcgc cgaccgcttt ccgcgaagct ccaatgttat
      721 actcgccgct cctataaagt ttatgcccta cagcctcaca cactggtctg agtttacgct
      781 acgaaacggc ttgcacctgc tgggcatagt ggcggccgcg ttgcttttga accggctgct
      841 gcgagtggtc accaacctgg tgatcaaacg cgcgtcgtcg ccgacccgcg cggcgcagat
      901 gcgcgaacaa cagacgcgca cactggcggg gctgctgttc agcgcgggca ccaaggtggt
      961 gtggggcatc gcgctgctta cggcgctgcc ggaattcggc atcaac
//
LOCUS       DATCBB010000199         2883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_104246, whole genome shotgun sequence.
ACCESSION   DATCBB010000199 DATCBB010000000
VERSION     DATCBB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104246"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VII37_02240"
     CDS             complement(<1..490)
                     /locus_tag="VII37_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5068568.1"
                     /translation="MLIGIFSELDAPGGVQRASRHIAAVLTEFAASRSMECRLFSLND
                     SPELHRMSVGGREFVFTGCERAKGRFAATAFRTARRHAKLVLAGHPNLAPVTQTMRLA
                     APRMKTIICTHGIEVWEPLSAVRRRSLRRANLVLAPSQSTAQHVITQQKVKPERVRVL
                     PWA"
     gene            complement(546..1217)
                     /locus_tag="VII37_02245"
     CDS             complement(546..1217)
                     /locus_tag="VII37_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5068569.1"
                     /translation="MATHNFSAVRALIFDLDGTLIDSKLDLVYSVNATLRHLHRPQLP
                     EETISSYVGNGAPVLVAKSLGPDVNPEELDRALKFFLNHYEEHKMDNTCAYPGVPEAL
                     RELSAMPMAVLTNKPVRISVRILEALGLAKYFRAIYGGNSFPTKKPDPLGVHTILREF
                     STTANEAVLIGDSEVDVQTARNAAILSAIVNYGFGIHDRVAHPADLYLDRLIDIVPAL
                     TATHS"
     gene            1329..2090
                     /locus_tag="VII37_02250"
     CDS             1329..2090
                     /locus_tag="VII37_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068570.1"
                     /translation="MHFVHSIDHHLLASVSIAGTCLDVLGTLYLAYDLLGGEHGPLRV
                     LTRAVTYSIVFGLGYAIGLGFFFGVVSGVATGVTVALELNRTARTGGHYSLPWEAFFS
                     AVRATGFAAGLYPMVGLRFVVAFGILITGGQVIAYSRGMRPALDYAAARRPRLSSRQF
                     WGTVIRTVGYSAAALGCSIFAQHLEHPWWFAIRVGVVTGLVTGVGILVNPYIEYYADR
                     LPQRTLGVLGVVLILCGFVLQSVQYWVALLDVPVI"
     gene            2186..>2883
                     /locus_tag="VII37_02255"
     CDS             2186..>2883
                     /locus_tag="VII37_02255"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY5068571.1"
                     /translation="MAKVFPFRAFRYNPSQAPFEEVLTQPYDKISPAMQDKYYAANPH
                     NLIAVEKGRVHPSDASGNNVYTRAAAAIQEWIRSGIVVQDPAASFYGYTQEYTVPGTQ
                     ERQTRRGFIGAGQLEEYSAGVIYRHEQTLSGPKADRLELLRHTQMATGQLFFIYSDAQ
                     RRADGVLNDAEKSAAPATEMTDEYGVIHRLWVISDAERVKAIQEAMADEKLVIADGHH
                     RYETALNYRNERRK"
BASE COUNT          599 a          649 c         1001 g          634 t
ORIGIN      
        1 gcgcccacgg aagaacgcga acgcgctcgg gctttacttt ttgctgcgtg atgacgtgct
       61 gggcggtgct ttgactgggg gcgagcacga ggttggcgcg ccgcaaggag cggcggcgca
      121 cggcggagag tggctcccat acctcgatgc cgtgcgtgca gataattgtt ttcatgcgcg
      181 gggcggccag gcgcatggtc tgagttacgg gggccaaatt tggatggccg gcgaggacta
      241 attttgcgtg gcggcgcgcg gtgcgaaaag cggtggcggc gaagcggccc ttggcgcgct
      301 cgcatccggt gaaaacaaat tcgcggccgc ctacagacat gcggtgaagt tccggggagt
      361 cgttgaggct gaaaagacgg cattccatgg aacggctggc ggcgaattcg gtgaggacgg
      421 ctgcgatgtg gcggctagcg cgttgaacgc cgccaggcgc gtccagttca gaaaatatgc
      481 cgatgagcac tgggtgagtc tgccaagcgc ggcgatggaa cgcaagcgcg agtactggaa
      541 atcactcagg aatgtgttgc ggtcagggcg gggacgatat cgatgagacg gtcgagatag
      601 aggtcggcgg gatgagcgac gcgatcatgg atgccaaagc cgtaattcac gatggcggag
      661 agaatggctg cgttgcgggc ggtttgcacg tcaacttcag agtcgccgat gaggacggct
      721 tcgttggcag tggtgctgaa ttcgcgaagg atggtgtgaa cgccgagagg gtcgggcttt
      781 ttggtgggga aactatttcc gccgtagatg gcgcgaaaat atttggctag accgagggct
      841 tcgagaatgc gcacgctgat gcgtacgggc ttattggtaa gcacggccat gggcatggcg
      901 gaaagttcgc gcaaggcttc gggtacgccg ggataggcac aagtgttatc cattttgtgt
      961 tcttcgtaat ggttgaggaa aaattttaga gcgcggtcga gctcttctgg attcacgtca
     1021 ggacccagtg atttggccac caggacgggc gcgccgttgc cgacatagct ggaaatggtt
     1081 tcttccggca attgcgggcg atgcaggtgg cggagcgtgg cgtttacgga atagaccaag
     1141 tcgagtttgg aatcgatgag ggtgccgtcg agatcgaaaa tcagggcgcg aaccgcggag
     1201 aaattatgag tggccattgg tatctaaata ttctaaagca ttaagaaaaa aacagaaacg
     1261 cgatgacatt ggctttgaag cggaagtaac ttttggagtg gaggcaactt cccttctaca
     1321 attagcgcgt gcatttcgtg cattccatcg atcatcactt gctggcgagt gtgagcatcg
     1381 cggggacgtg cctggatgtg ctgggcacgc tgtaccttgc ttacgatttg ttgggcggcg
     1441 aacacggacc actgcgggtg ctgacgcgag cggtgacgta ctcgatcgta tttggtcttg
     1501 gatatgcgat tgggctggga tttttctttg gcgtggtttc cggtgtagct actggagtca
     1561 cggtggcgct cgaactgaat cggacagcgc ggacgggagg tcattactcg ttaccttggg
     1621 aagctttttt tagcgcggtg cgcgcgacag gttttgccgc gggactttac cccatggtgg
     1681 ggctgcggtt cgtggtggct tttgggattt tgatcactgg cggccaggtc atcgcctatt
     1741 cgcgtggcat gcggccagca ttggattatg cggctgcgcg gcggccgcgg ctttcgagca
     1801 gacaattttg gggcacggtg atcagaaccg tgggctacag cgccgcggca ttgggctgta
     1861 gtattttcgc gcagcatctg gaacacccgt ggtggtttgc gatacgcgtc ggagtggtga
     1921 caggactggt taccggggtc ggcattcttg tgaatcctta catcgagtat tacgcggacc
     1981 ggttgcctca gcggacgcta ggagtgcttg gagtcgtgct catactttgt ggattcgtgc
     2041 tgcaatcggt tcaatattgg gtggcgttgc tggacgtgcc ggtaatttag ttgcgaacga
     2101 tttatgcgcg cgggtgaaga cggcgttggg cggcgagttc gcgtaaacta gacgactaca
     2161 cattcagaaa atttccggag aatacatggc caaggttttt ccgttccgag cattccgcta
     2221 taacccctcg caagcgccct ttgaagaggt gcttacgcag ccgtatgaca aaatttcgcc
     2281 ggcgatgcag gacaaatact acgcggccaa tccgcacaat ctgatcgcgg tggaaaaagg
     2341 gcgcgtgcat ccgagcgacg cgtccggaaa caatgtgtac acgcgggcgg ctgcggcgat
     2401 acaggagtgg atccgcagcg gaattgtggt gcaggatccg gcggcgtcat tttatgggta
     2461 cacgcaggaa tataccgtgc cgggaacgca ggagcgtcag acgcggcgcg ggtttatcgg
     2521 ggctgggcag ctggaggaat attccgcggg tgttatttac cggcacgaac aaacgctttc
     2581 ggggccaaag gcggaccggc tggaattgtt gcggcacacg cagatggcta cggggcaatt
     2641 attttttatt tattcggatg cgcagcggcg ggcggatggg gtgttgaacg acgcagagaa
     2701 gtcggcggct ccggcaacgg agatgacgga tgagtatgga gtgattcacc gattgtgggt
     2761 gatttccgac gcggaacggg tgaaagcaat tcaagaagcg atggcggacg aaaaactggt
     2821 gattgcggac gggcatcacc gttatgaaac ggcattgaat tatcgaaacg agcgtcgcaa
     2881 gga
//
LOCUS       DATCBB010000200         4155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_330819, whole genome shotgun sequence.
ACCESSION   DATCBB010000200 DATCBB010000000
VERSION     DATCBB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330819"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VII37_02260"
     CDS             complement(<1..674)
                     /locus_tag="VII37_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068572.1"
                     /translation="MNLRRVLAFAFVLLLAMGVRHATAHPAPDTVDSNDYATADAQIL
                     KEIHDHSEAMENLEHLSDAIGARLTGSPQLKQANEWTAEMFRKYGLTNVHLEPWTIAH
                     SWTRGTAHARIVKPAEHPLTIAAAGWSPSTPGVLHAPVVYFDAKKKDEFEKFRGKLKG
                     AVVIYQEPASLSPPKPEDSRSEVVRAMQAPPPMKGEPPAPSPYAVLQEAAKARTAFFK
                     QEGVAAV"
     gene            complement(722..1705)
                     /gene="mutM"
                     /locus_tag="VII37_02265"
     CDS             complement(722..1705)
                     /gene="mutM"
                     /locus_tag="VII37_02265"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY5068573.1"
                     /translation="MPELPEVETVARGLRLTVLGRRILSVTLRKTDFIDDPSLIERHL
                     PGRRIEAVERYGKFMLLRLSAAQGEENSANGDNAPSALLVHLGMTGHIASTAVAEPWE
                     KHTHASFALDDGRELRYTDARRFGRLAYFSAALLPAELEGFGADPLEVTAEEFATRIR
                     SRNSRIKSLLLDQTVLRGVGNIYADESLWRAKIHPAKLGASINKKQTAVLHRALQDIL
                     KKAIVMRGSSIANFVDGDGNPGGYQQHHRAYGREGEKCYRCGTIIRRAIVAGRSSYFC
                     PKCQTGPRGFSALSFTPPKSRRDSAARDRKKNIKPPGKRPKRARSSRVRTK"
     gene            complement(1778..>4155)
                     /locus_tag="VII37_02270"
     CDS             complement(1778..>4155)
                     /locus_tag="VII37_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5068574.1"
                     /translation="EKGNTRNLTRTAAVAERDPSWSPDGKWIAFFSDESGEYALYLVD
                     QSGLGTVKKIDLGKPASFFYGPTWSPDSKKIAFSDKRLNLWCVDTEKGSSTPVKIDTD
                     LYDAWGVAMSPAWSPDSKWLTFTKFLPNHLRAVFVHSLETGKTSQITDGLSDARYPVF
                     DKGGKALYFAASTDLGLAMTWLDLSGFQRPVSRNIYAVVLKKTDPNPIEPESDDEKPD
                     AAKTADKSKDTDKKTEDKDKDKDKSKDKKKTEADKTKSKDDDKSKDSDKKEEPVVVTI
                     DLDNIGQRIVSMPIKASNYVALDTGKEGVLFLTETPDIPPLNAPPTATVTKFDLKTRK
                     TEPFLTGVSAFAVSANGEKVLYHTGQGPTWFIAATGAAPKAGEGALKFDEMEVKVDPR
                     AEWNQMYHEVWRIERDFLYDPHFHGLNLAAAEKKYTPYLKGAGGRADVNHLFVEMLGE
                     VTIGHMFIFGGDIPKTPEVKSGLLGADYKVENGRYRFAKIFNGENWNPQLRAPLTQPG
                     VDVKLGDYLLEVNGAEVRPPNDVYSYFENAAGKQTRIKVGPNPDGKDARDVTVVPIAD
                     EAELRTRAWEEDNRRKVSELSGGKVAYVHVPDTANGGFLNFNRFYFAQTDKQAAVIDE
                     RYNHGGAIADYIIDLLERPLRNCATTREGEKWCSPLAQIYGPKTMVINEMSGSGGDAL
                     PWMFRQDKIGPLVGTRTWGGLVGIGGYPSLLDGGGVMAPRAAIYGLHGEWEVENHGIA
                     PDIEVENDPASVAAGHDAQLEKAVQVTLEALKKNPVVIPEHPPYPNYHKTE"
BASE COUNT          796 a         1110 c         1202 g         1047 t
ORIGIN      
        1 accgcggcca cgccttcctg tttgaagaag gctgtgcgcg cttttgcggc ttcttgcaag
       61 acggcgtatg gcgaaggggc tggcggttcg cccttcatgg gcggaggcgc ctgcatggcg
      121 cgaacaactt cgcttctgga atcttcgggt ttgggcgggg acaagctggc gggctcctga
      181 taaatcacca ccgcgccttt tagctttccg cgaaatttct cgaactcatc tttctttttt
      241 gcatcgaagt aaaccaccgg cgcgtgtagc acgccgggcg tgctgggcga ccaaccggcg
      301 gcggcgatgg tcaacggatg ctcggcgggt ttcacgatgc gcgcgtgcgc tgtgccgcgg
      361 gtccaggagt gcgcgatggt ccagggctcg agatgtacgt tggtgaggcc gtacttgcgg
      421 aacatttccg cggtccactc gttggcttgt ttcaattgtg gcgaaccggt gaggcgcgcg
      481 ccgatggcgt ctgataaatg ttccagattt tccatggctt cgctgtggtc gtgaatctct
      541 tttagtattt gagcgtcagc agtcgcgtaa tcgttcgagt cgactgtatc gggggcggga
      601 tgagccgtgg catgacggac gcccatcgct aacagcagca cgaaggcaaa agcgagtact
      661 cgacgtaggt tcatccgcgc tccctgacag agttttctgc gtgcaaaggt cttctagcga
      721 attatttcgt tctcactcta ctgctacgtg cccgcttcgg ccgctttcca ggcggcttga
      781 tatttttctt gcgatcgcga gctgcagaat cgcgcctcga tttaggcggc gtgaacgaca
      841 gagccgaaaa accgcgcggg ccggtctgac acttcgggca aaaatagctg ctgcggcccg
      901 cgacgatggc cctgcgaatg attgttccgc agcggtagca cttttcgcct tcacggccgt
      961 aggctcgatg atgttgctga tatccgccag gatttccgtc tccatccaca aaattggcga
     1021 tggaagagcc gcgcatcacg atggcttttt tcagtatgtc ttgcaaggcg cgatgaagga
     1081 ctgcagtctg ttttttattg atactcgccc ctaactttgc gggatgaatt ttggcgcgcc
     1141 ataaactttc atcggcgtat atgttgccca ctccgcgtag cacagtctga tcgagtagaa
     1201 gcgacttgat acgggagttg cgtgagcgga tgcgcgtggc aaattcctcg gcggttactt
     1261 ccagcggatc ggcgccgaag ccttcgagtt cggccggcaa taatgccgcg gagaagtagg
     1321 ccagccgccc gaatctgcgc gcgtcggtgt agcgcaattc tcttccgtcg tctaaagcga
     1381 agctggcgtg ggtgtgtttt tcccatggct cggcgacggc ggtggaagcg atgtgaccgg
     1441 tcatgccgag gtgaacgagc aacgccgacg gagcattatc tccgtttgcg gaattttctt
     1501 ccccctgagc ggcagacaga cgcagcagca taaatttgcc gtagcgttcc accgcttcta
     1561 tgcggcggcc tggcaagtgc cgctcgatta aagaaggatc gtcgatgaag tcggtttttc
     1621 gcagcgttac ggacaggatc cggcggccga gcacagtcag ccgcaagcca cgtgcaacgg
     1681 tttcgacttc gggaagttca ggcatgaaga gtgttcacga aaatatcaca acgcgcgtca
     1741 ccattggcca ctcgcgcgag acaatcacca agccgcatca ctctgtcttg tgataattgg
     1801 ggtacggcgg atgctccggg atgactacgg gattcttttt tagagcttcc agcgtgacct
     1861 gcacagcttt ttccaattgc gcgtcgtgcc cggcggcgac agacgcagga tcattttcca
     1921 cttcaatatc cggcgcgatt ccgtgattct ctacttccca ctcgccgtgc agaccataga
     1981 tggcagcgcg tggggccatt actccgccgc catccaataa cgatggatat ccgccgatac
     2041 cgaccagtcc gccccaggtg cgcgtgccta cgagcggacc aatcttatcc tgacgaaaca
     2101 tccagggaag cgcgtcgcca ccggagccgg acatttcgtt gatgaccatt gtttttgggc
     2161 cgtaaatttg cgcgagaggc gaacaccatt tttcgccttc gcgggtggtg gcgcagtttc
     2221 gcagcgggcg ttcaagcaaa tcgatgatgt aatcggcaat cgcgccaccg tggttgtaac
     2281 gctcgtcgat gacggcggcc tgtttgtcgg tttgcgcaaa ataaaaacga ttgaaattca
     2341 ggaatccgcc gttggcagta tcgggcacgt gcacataggc gacttttccg ccgctgagct
     2401 ctgacacttt gcggcgatta tcttcttccc acgcgcgcgt gcgcagctca gcctcgtcgg
     2461 ctatgggcac cacagttaca tcgcgtgcgt ccttgccgtc gggatttgga ccgaccttga
     2521 tgcgggtctg cttcccagcg gcattttcaa aatagctgta gacatcgttg ggtgggcgca
     2581 cttcggcgcc atttacttcc aataaataat cgcccagctt cacgtccacg cccggttggg
     2641 taagcggggc gcgtaattgt gggttccagt tctcgccgtt gaatattttg gcaaagcggt
     2701 aacggccatt ttccacttta tagtccgcgc ccaagaggcc gctcttaact tccggcgttt
     2761 ttggaatgtc acctccaaaa ataaacatgt ggccgatggt tacttcaccg agcatttcca
     2821 cgaaaagatg attcacgtca gcgcggccac ctgcgccttt taggtacggg gtgtatttct
     2881 tttcagcagc ggccaaattg aggccgtgaa aatgcgggtc gtacagaaag tcgcgttcga
     2941 tgcgccacac ttcgtggtac atctgattcc actccgcgcg tggatcgact ttcacttcca
     3001 tttcgtcgaa cttgagggcg ccttctccgg ctttgggagc tgcgccggtg gcggcgatga
     3061 accacgtggg gccttgtccg gtgtgataca aaactttttc tccgttggcg gagacggcga
     3121 aggcgctgac gcccgtgagg aacggttcag tttttcgagt cttaaggtca aatttcgtaa
     3181 cggtggccgt gggcggagca ttcagtggag ggatgtccgg agtttcggtg aggaaaagaa
     3241 cgccctcttt cccagtatcc agagcaacgt aattggaagc tttgatgggc atggagacga
     3301 tgcgttggcc gatattatcg agatcgatgg ttactacgac gggctcttct tttttgtcgc
     3361 tatctttgga tttgtcgtcg tctttgcttt tagtcttgtc tgcctcggtt ttcttcttat
     3421 ctttggattt gtccttatcc ttgtccttgt cttcggtctt tttatcagtg tctttggatt
     3481 tgtcggcggt tttcgcggca tccggttttt cgtcgtcgct ttccggctct atgggattgg
     3541 gatcggtctt cttcaggact acggcataaa tattgcgcga taccgggcgt tgaaatccgg
     3601 acaggtcgag ccacgtcatg gccaggccaa gatccgtact cgcggcgaag tacagtgctt
     3661 tgcctccctt gtcgaagact ggatagcgcg catcgcttaa gccgtcggta atttgcgaag
     3721 tctttccggt ttcaagggaa tggacgaaca cggcgcgcag gtgattgggc agaaattttg
     3781 tgaaggtgag ccatttgctg tctggcgacc acgcggggct catagccacg ccccaagcgt
     3841 cgtacaaatc ggtgtcgatt ttaaccggcg tggaagagcc tttttcggtg tccacgcacc
     3901 ataggttcaa acgcttgtcg ctaaaagcga ttttcttgct gtccggcgac cacgttgggc
     3961 catagaagaa agatgccggt ttacccaagt cgattttttt gactgttcca agcccggact
     4021 ggtccaccag gtacaaagcg tattcgccgg attcatcgga aaaaaatgcg atccacttgc
     4081 catcgggcga ccatgaaggg tcgcgttcag ctaccgcggc agtgcgcgtt aaatttcgcg
     4141 tgttgccttt ctccg
//
LOCUS       DATCBB010000201         1097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_396017, whole genome shotgun sequence.
ACCESSION   DATCBB010000201 DATCBB010000000
VERSION     DATCBB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396017"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VII37_02275"
     CDS             complement(<1..1059)
                     /locus_tag="VII37_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068575.1"
                     /translation="MKKSSLAATWAAESYREKLVTRFWLELPPREKSLVLRGPDLWSV
                     PVVALRQTLGNLADAGADAPRREARHIVLNYARRLEHEDPRARQAVAAGLNEFLPIIE
                     SLWPNQLPEDLSKVALTALQIERQPQTSAALATFIESLSHIAVVRGDYDGFESILLAL
                     EQSSGDFDQANLSTLAKRLFAQDRVFLLVDAGLAHRALDPVLPRLLVRDPDKLLDRLT
                     FLLGEVRGASLLPAMARLIRTIGVPVLTILETRLYEARPQRVMAAIKLLAAADPDRLL
                     RGLTRALGSWEWNMQDLAISELSRPTNAPNAQTAAFVFSTVLTNAHPLVVPMMIDQIG
                     LSGESSAIPQLMEIASGEH"
BASE COUNT          248 a          331 c          309 g          209 t
ORIGIN      
        1 gtgttcgccg gaagcaatct ccatcaactg cggaatcgcg gagctttcgc cactcagccc
       61 aatctgatcg atcatcatcg gcacgaccag cgggtgagca tttgtaagca ccgtggaaaa
      121 tacgaacgcg gcagtctgcg cgttgggagc gttggtcggc cgcgatagtt cgctaatcgc
      181 caaatcctgc atgttccatt cccagctgcc caaagctcgg gtgaggccgc gcaataatcg
      241 gtccgggtcc gcggccgcaa gcaacttgat ggcggccatc acccgctgcg gacgagcttc
      301 gtaaagacgc gtctcgagga tggtcaacac cggcacgccg atggtccgta tgagacgcgc
      361 catggctggc aacaagctcg cgccgcgaac ttcacccaac aaaaacgtaa ggcggtccag
      421 caatttatcg ggatctcgaa ccagcaatct gggcaacacc gggtccagcg cgcggtgcgc
      481 aagtccagca tccacgagca ggaacacccg gtcctgcgcg aacagacgtt tggccagcgt
      541 agagaggttg gcctgatcga aatcaccgga agactgttct aatgccagga ggatcgattc
      601 gaagccgtca taatctccgc gtaccaccgc gatgtggctc aatgattcga taaatgtggc
      661 tagcgcagca gatgtttgcg gttggcgttc gatttgcagc gctgtcagcg cgaccttgct
      721 taaatcttca ggcaattggt tgggccacaa cgattcgatg atcggtaaga attcattaag
      781 ccccgcagcg actgcctgtc gcgcgcgagg atcctcatgc tcaagccgcc tggcataatt
      841 caatacgatg tggcgtgctt cacggcgcgg cgcatcggcg ccggcatctg cgagattgcc
      901 caatgtttgg cgcaacgcca ccaccgggac actccacaaa tcaggcccgc gcaacaccag
      961 cgacttttcg cgcggcggga gctccagcca aaagcgagtc accaattttt cgcgataact
     1021 ttccgcagcc cacgtcgctg ccaaggaaga cttcttcatc ggagccgaag gagcctgttt
     1081 acaaggacca tctgcgc
//
LOCUS       DATCBB010000202         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_104298, whole genome shotgun sequence.
ACCESSION   DATCBB010000202 DATCBB010000000
VERSION     DATCBB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104298"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VII37_02280"
     CDS             <1..694
                     /locus_tag="VII37_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5068576.1"
                     /translation="SVTGVRRGGGARKLPKLRLGIPREFFFDVISREVQKSFDAAVRA
                     MRALGAKIKDVSIPLLAQTEQAGNRIAWAEASYYHQQSGWFPDRAAEYSEDVRSRLQM
                     GTKVSAVEYLEAQEQRRTFISQLREVMEKENLDALMVPTTPIAAPLIGEETTRVGKID
                     YPTRALLLRLNRPANLAGVPAISVPCGFTAAELPIGLQFIGAENDEQLLLDIVNSFQR
                     SNPFKIPTNFRA"
     gene            809..1675
                     /locus_tag="VII37_02285"
     CDS             809..1675
                     /locus_tag="VII37_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY5068577.1"
                     /translation="MSDVSLAGAFLAGLVSFLSPCVLPLVPGYISMLSGIGMEQLRKG
                     ELPRSGLFGSSLAFVAGFSAVFISFGASASAVGSFLRQNRGVLAPIAGALILLFGLHL
                     LGLLIKLTLRTGLIIGAVLVVLGIISLLRHGPLFAGFGAIHFFSLSLIGFFGPSMARW
                     LNRDVRIQSKIGQPGVWSAFLLGFAFAFGWTPCIGPILATVLALAAASDTIARGILLL
                     AVYSAGLAVPFLLTALGIGQFLRFYQNFRKYLHAVEVFSGALLIFVGGLVFVNKLTWL
                     SGKLTFLNRFSL"
     gene            1705..>2241
                     /locus_tag="VII37_02290"
     CDS             1705..>2241
                     /locus_tag="VII37_02290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5068578.1"
                     /translation="MKNVGVIVATVLFLIGATYYADKKTRVSNNAAAHKDYSPDKSIG
                     MPAPTVHFKDLDGKDASLGDYNGKVVLVNFWATWCDPCREEIPWLIEMQQKYAGKGFT
                     VLGIAMDEEGKSAVAPFVSKEHFDVSNQKLTMNYPIVIGNDDIADKFGGLLGYPTSVL
                     ISRDGKQVKRITGIISYEE"
BASE COUNT          473 a          509 c          674 g          585 t
ORIGIN      
        1 atcagttaca ggcgtccgtc gtggcggtgg cgccagaaaa cttcctaaac ttcgcctcgg
       61 gatacctcgg gaatttttct tcgatgtaat ttcccgagaa gtgcagaaaa gttttgacgc
      121 cgcggttcgg gccatgcgcg cactcggagc aaaaatcaaa gatgtctcca taccgctttt
      181 ggcgcaaacc gaacaggcgg gcaatcgcat cgcttgggcg gaggccagct attatcatca
      241 gcaatcggga tggtttcccg atcgtgcagc ggagtatagc gaggacgttc ggtcgcggct
      301 tcaaatgggg acgaaagttt cggcggtgga atatctggaa gcgcaggagc agcgcagaac
      361 cttcatttct caattgcgtg aggtgatgga gaaagaaaac ctcgatgcgc tcatggttcc
      421 aaccactccg attgcggcac cactgattgg cgaagaaaca acccgcgttg gaaaaatcga
      481 ctatcctacg cgggcgctgc tattaaggct aaatcgcccc gcgaacctgg cgggtgtgcc
      541 ggcgatttcg gttccatgcg gtttcacggc ggcggaattg ccaattgggt tgcaatttat
      601 cggcgcggaa aacgacgagc agttgttgct ggacatcgtc aacagtttcc agcgttcgaa
      661 tcctttcaag ataccgacca atttcagggc ctagcccgta tcatttgccg cggaagtgcg
      721 tctaacgcat ttatgggaga atggagtgta cgcgcgggtg gttcggaccg gataccacga
      781 tgaccatatt tcacgggaac gctgaggtat gagcgacgta tcgcttgccg gggcatttct
      841 tgccggatta gtttcgtttt tgtcgccttg cgttttgccg ctggtacctg gctacatttc
      901 catgttgtcg ggaattggca tggagcaatt gcgcaagggt gaattgccac gctccggatt
      961 gtttgggtct tcgctggctt ttgtggcggg attttccgcg gtgtttattt ctttcggggc
     1021 gtcggcgagc gcggtggggt cgtttttgcg ccaaaaccgg ggcgttctgg cgcccattgc
     1081 gggcgcactg attttgctat ttgggcttca tcttttggga ttgctgatca agctcacgtt
     1141 aagaaccgga cttatcatcg gcgcggtact ggttgttctc gggattatct cgttgttgcg
     1201 ccatgggccg ctgtttgcgg gttttggcgc gatccacttt ttttcgttgt cgctgattgg
     1261 tttttttgga ccgtcgatgg cgcgctggct taaccgcgat gtgcggatac aaagcaaaat
     1321 cgggcagcca ggcgtatgga gcgcgttttt gttgggattc gctttcgcat tcggctggac
     1381 gccttgcatc gggccaattc ttgcaacggt tttggcgctt gcggcggcca gcgacacgat
     1441 tgcgcggggg attttacttt tagcggtcta ttccgcggga ctggcggtgc cttttctact
     1501 taccgcgctg ggcatcgggc agtttctgcg cttctatcaa aattttcgca aataccttca
     1561 tgcggtcgaa gtttttagcg gggcgctgct gatttttgtc ggcgggctag tgtttgtaaa
     1621 caaattgacc tggctctcag ggaaactgac ttttctgaac cggttctcac tttagggcgc
     1681 acggtttaag attaggaaat gaaaatgaag aatgttggcg tgattgtggc aaccgtgctg
     1741 tttctgatcg gggcgacgta ttatgcggat aaaaagacgc gggtatcgaa taatgcagct
     1801 gcgcacaagg attattcgcc ggataaatcg atcgggatgc cagcgccgac cgtgcatttc
     1861 aaagacctcg atgggaaaga tgcttcactg ggcgattaca acgggaaagt ggtactggtg
     1921 aatttttggg ctacgtggtg cgatccctgc cgagaggaaa ttccgtggtt aatcgaaatg
     1981 cagcagaaat atgcgggcaa agggtttacg gtgctgggca tagcgatgga tgaagaggga
     2041 aaaagcgcgg tggcgccatt tgtatcgaag gagcattttg acgtcagcaa tcaaaaactg
     2101 actatgaatt atccgatcgt gatcgggaat gacgacattg cggataaatt tggcggtctg
     2161 ctcggatacc ccacgagtgt tctaatctcg cgagatggca agcaggtaaa acgaattacc
     2221 ggcatcatca gttacgagga a
//
LOCUS       DATCBB010000203         1514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_266199, whole genome shotgun sequence.
ACCESSION   DATCBB010000203 DATCBB010000000
VERSION     DATCBB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266199"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..339)
                     /locus_tag="VII37_02295"
     CDS             complement(205..339)
                     /locus_tag="VII37_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068579.1"
                     /translation="MGQDNKCCGSDHRFNSGMQDCGGSRQELMVNGAKIRIESRKEIV
                     "
     gene            338..1498
                     /locus_tag="VII37_02300"
     CDS             338..1498
                     /locus_tag="VII37_02300"
                     /inference="COORDINATES: protein motif:HMM:NF021285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEY5068580.1"
                     /translation="MPSRYEQWMYDWETRLTSVDSNRIVRPLEWGAEWAQDWPCRNGC
                     KPGTLPEDNETHLREYNRRIVASSDEFFSYATPSDFRLENREVQVFSTREIPDPRLEA
                     KVRGTYANFLRFTSPAHTPFPENNLANARWFPAKGNRAVVLLPHWNSDALAYNTLCRV
                     LNRLGIAVLRLSMPYHDIRRPAGVHRADYAVSANIGRTVDAARQGVVDVRCCLDWLEQ
                     QGYTQLGIVGTSLGSCYAFLAAAHDSRIRVAAFNHASTYFADVVWHGQSTRHIREGLE
                     PHLDVERLRDLWSAISPMSYFKQFARWPKKSLIVYAKYDLTFLPEFSREVVAEFERYG
                     LDHKVAVLPCGHYTTGETPYKYMDGWHLGSFLRRAFANLPRTRRDRRPRLSS"
BASE COUNT          317 a          442 c          410 g          345 t
ORIGIN      
        1 aatcggagaa atcttcttcg ccgacgataa aagtgcgctt cctctgcggc gcatcgtgcg
       61 gggaatttcg cgagtgtatc gcggcgaaag agtggaaccc cagaactatc acgtaggggt
      121 ggacgccctc gtccgcccag cggagtgaaa ctccgcagtt tttggcaggc tacaactcgc
      181 caccccggac gagggcgtcc ggggctacac aatctctttt cgcgactcga tgcgaatttt
      241 ggcgccattc accatcaatt cctgtcggct gccgccgcaa tcctgcattc cgctgttgaa
      301 tcgatgatcg ctgccgcaac acttgttatc ctgacccatg ccttcgcgct acgagcaatg
      361 gatgtacgac tgggaaacca ggctgacctc ggtggacagc aatcgcattg tgcgtccgct
      421 ggagtggggg gcggagtggg ctcaagactg gccctgccgg aatggatgca agccgggaac
      481 tcttccggaa gacaatgaaa cccatttgcg cgaatacaac cggcgcatcg tggcgtccag
      541 cgatgaattt ttctcctatg cgacgccgtc tgactttcgc ctggagaatc gcgaagtcca
      601 agtcttcagc acccgcgaaa ttcctgatcc gaggttggaa gctaaggttc gcggaaccta
      661 cgccaacttt cttcgcttca cttccccggc gcacactcct tttccagaaa ataatctggc
      721 aaatgcacga tggtttccgg ccaaggggaa tcgtgccgtg gtgctgctgc cgcattggaa
      781 ttcggacgcg ctcgcctata acactttatg ccgcgtcttg aaccggctcg gcatcgcggt
      841 tctgcgtttg agcatgccct atcatgacat tcgcaggccg gcgggagttc atcgtgccga
      901 ctatgctgtt tccgcgaata tcggacgcac tgtcgacgcc gcacgacagg gagtcgtcga
      961 tgtccgctgc tgcctggatt ggctggaaca acagggctac actcagcttg gaatcgtggg
     1021 cacgagcctc ggatcctgtt acgccttcct tgctgctgcc catgattctc gaatccgcgt
     1081 cgccgctttc aatcacgcgt caacttactt tgcagatgta gtttggcacg gccaatccac
     1141 tcgccacatt cgcgaaggtc ttgaaccgca cctcgatgtc gaacgcctgc gcgatctctg
     1201 gagcgcgatc agtcccatgt catatttcaa acagtttgcc cgctggccca aaaaatcgct
     1261 aattgtttat gcgaaatacg atctgacgtt tcttcccgaa ttctcgcgcg aggtggtggc
     1321 ggaatttgaa cgctatggac tggatcacaa agtggcagtc ttaccctgcg gccattacac
     1381 aaccggagaa acaccgtata aatatatgga cggctggcat ttggggtcgt ttctgagacg
     1441 agccttcgcc aatcttccga gaacacgtag ggaccgccgc cctcggctgt ccagttgagc
     1501 gaagctcaac agag
//
LOCUS       DATCBB010000204         1095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_363254, whole genome shotgun sequence.
ACCESSION   DATCBB010000204 DATCBB010000000
VERSION     DATCBB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363254"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VII37_02305"
     CDS             complement(<1..786)
                     /locus_tag="VII37_02305"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068581.1"
                     /translation="MSPDSDLAREEKSPGKAGDGVAITGWAAALGAQRVLSEEVDRAF
                     AMPIGKLRTRAGIESVSYAAQGETELTLGAQAMQQALSVAGCEASDIDWIIASSETHI
                     EYPSLAAQLHKRTKVREKCGVLDAGGACLGLLNALAVAQSFVDSGRARNVAVVTADVH
                     SRTLVPGRVAGEFGGLFGDGASAFVLRNARGVEKSAGYFLGRFFFGCANQYADAICVA
                     GVENGGLAVRFDGEALSRAAITRMERTLTEVEKESGICRWEVGA"
     gene            complement(776..>1095)
                     /locus_tag="VII37_02310"
     CDS             complement(776..>1095)
                     /locus_tag="VII37_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY5068582.1"
                     /translation="RHEPLLAPTAELRKSYQEDKDWPAYEQGFLKLLKQRGVPKSLDM
                     SDWPANVALLCTEPGPEKCHRRLVAELLVKHWRAANDDVEVRHLVANTRGRRGKHGTA
                     NES"
BASE COUNT          236 a          395 c          238 g          226 t
ORIGIN      
        1 cgcgccaacc tcccagcgac aaataccgct ctccttttcc acctccgtca gcgtccgttc
       61 catccgcgta atcgccgccc gcgacaatgc ctccccatcg aatcggaccg ccagccctcc
      121 attctcaacg ccggccacac aaattgcgtc cgcatattga ttggcgcacc cgaagaaaaa
      181 tctccccaag aaatagccag cggatttttc aactccgcga gcattgcgca aaacaaatgc
      241 gctagcccca tcgccaaaca atccgccaaa ttcccccgcg acacgccctg gcaccaatgt
      301 gcggctgtga acatccgcag tgaccaccgc cacgtttcgc gcccggccac tatcaacaaa
      361 agactgcgcc accgccagcg cgttcaacaa gcccagacaa gcgcctccgg catccaacac
      421 cccgcatttt tcacgcacct ttgtgcgttt atgaagttgc gcggctagcg atggatattc
      481 aatatgcgtc tcgctgctcg cgatgatcca gtcaatgtcg ctggcctcgc atccagcaac
      541 actaagcgcc tgctgcatcg cctgcgcccc gagagtaagt tccgtttccc cctgggcggc
      601 atacgacacc gactcaattc ccgcgcgagt tcgcaatttc ccgatgggca tggcaaaagc
      661 gcgatccact tcttccgata acacgcgctg ggctcccaat gccgccgccc agccagtaat
      721 cgcgacacca tcacccgcct tgcccggcga tttttcttcg cgagccaaat ctgaatcagg
      781 actcattagc tgtgccgtgc ttccctcgcc gacctcgcgt attggcgacc aaatgccgca
      841 cttccacatc gtcgttcgcc gcgcgccagt gcttcaccag caattcagca accagccgcc
      901 ggtgacattt ctccggcccc ggttcagtac atagcaacgc cacattagcg ggccagtcgg
      961 acatgtcgag acttttcggg acaccacgct gcttcaataa ttttaaaaat ccttgctcgt
     1021 aagcaggcca gtctttatct tcctggtagg attttcttag ctccgccgtc ggtgccaaca
     1081 acggttcatg cctgt
//
LOCUS       DATCBB010000205         1070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_298739, whole genome shotgun sequence.
ACCESSION   DATCBB010000205 DATCBB010000000
VERSION     DATCBB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298739"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VII37_02315"
     CDS             <1..315
                     /locus_tag="VII37_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068583.1"
                     /translation="PENLEAPVLWGKEEVVAERFSKVGWKVKTERRNLEFKYPFPAKD
                     VVKLFRECFGPTKVAFSRLDAIGQQGLAADLEKLWAEHNEGSASETFVKAEYLEVIAR
                     KA"
     gene            complement(524..>1070)
                     /locus_tag="VII37_02320"
     CDS             complement(524..>1070)
                     /locus_tag="VII37_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5068584.1"
                     /translation="RPFSMQLAVRTTSQPASVTSAVTAAIHSIDPTQPVVDVMTMDDL
                     IATSLSQRRFSMFLLAAFAVLALLLAAVGIYSVLSFAVRRRVREIGIRVALGAETKDI
                     LRLVVTEGMKPALLGLALGVAGALALGRVVASFIYGIAPYDPLTFAAVAALLATVALL
                     ASIIPAFRAARIEPTRALREE"
BASE COUNT          245 a          247 c          364 g          214 t
ORIGIN      
        1 ccagaaaatc tggaagcgcc ggtactgtgg ggcaaagagg aagttgtggc ggagcgattt
       61 tctaaagtgg gttggaaagt aaagacagag cggcgaaatc tggaattcaa atatccgttt
      121 cccgctaagg acgtggtgaa attgttccgg gaatgttttg ggccgacgaa ggtggctttt
      181 tcgcggctcg acgcaatcgg gcagcaaggg ctggctgctg atctggagaa actttgggcc
      241 gagcataacg aaggatctgc gagcgagaca tttgttaagg cggaatatct ggaagtgatc
      301 gcacggaaag cttagttttt ttggtgaggt ggacaatttc cgcggagttg gaattagtga
      361 gtcgcgacct atcgaatggg cgaagaagaa acgcgtagag ctccgcggat cgttattgat
      421 ggttccacgt tacaagacgc acctgccacg gcaggtaaac cctccaaaaa accgagggtg
      481 gggcaccctt ctatgacgtt gtcggctttg tcaccgcgtg actttattcc tcgcgaagcg
      541 cgcgggttgg ttcgattctg gcggcgcgaa atgcggggat tatgctggcc agcaacgcta
      601 cagtggcgag cagggcggct acggcggcga aggtgagtgg gtcgtagggc gctatgccgt
      661 agatgaagct ggcgacgacg cggcctaagg cgagggcacc tgctacgccg agtgccaggc
      721 cgagtaacgc gggtttcatt ccttctgtta caaccaaacg caaaatgtct ttcgtctcgg
      781 cgccgagggc tacgcggatg ccgatttcgc gcacgcggcg gcgcaccgcg aaagagagca
      841 cgctgtagat tcccacggcg gcgagcaaca acgcgaggac cgcgaatgct gcgagcaaaa
      901 acatgctgaa gcgtcgctgg gaaagagagg tggcgatgag atcgtccatg gtcatgacgt
      961 cgactactgg ctgcgtggga tcgatcgaat gaattgcggc ggtcaccgcg gaggtcacgc
     1021 tcgctggttg tgaagtggtg cgcacggcga gttgcatgga aaacggacgc
//
LOCUS       DATCBB010000206         1113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_428641, whole genome shotgun sequence.
ACCESSION   DATCBB010000206 DATCBB010000000
VERSION     DATCBB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428641"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..557
                     /locus_tag="VII37_02325"
     CDS             57..557
                     /locus_tag="VII37_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068585.1"
                     /translation="MSIDPHLDPKDRPDRTVQGAHAVHGALPHHDTVAYEQRDIKVRS
                     IYWYLIALTISVAASFFICIYILRYTERFVSRHESPMMPARAAKGPDYRVMPPEPRLQ
                     GVPGHDADPQQDHRDKVRADNDANEKYGWVDQNAGIAQIPVKEAMKIIAQKGLPATSP
                     AAPEKK"
     gene            561..>1113
                     /locus_tag="VII37_02330"
     CDS             561..>1113
                     /locus_tag="VII37_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5068586.1"
                     /translation="MNRARAIATIALLLLSGTIARSQAVPSNIGVTSTTMPAQLQNVG
                     FEPQLNAQMPLDLPFVDENGSNVQLRDYFKQKPVVLAFVYYGCPMLCNQVEQGVVGSL
                     RMLSFIPGRDYEVVFVSFDPRESADMAAQKKKSAMEHFRRPETASGWHFLTGTKESID
                     VATKAANFRYSFDTKNNLFAHASG"
BASE COUNT          259 a          328 c          283 g          243 t
ORIGIN      
        1 aaagctgccg ttgctgccgc tcggcgaccc taaacttgcg gaggccattg ctcatcatga
       61 gcattgatcc acatctcgat cctaaagatc ggccggatcg caccgtacag ggcgctcatg
      121 ccgtccatgg cgcattgccg caccatgaca ccgttgcgta cgaacagcgc gacataaaag
      181 tgcgcagcat ctattggtac ttgatcgccc tgaccatatc cgtggccgcc tcgtttttta
      241 tctgtatata cattcttcgt tacaccgaac gttttgtgtc gcgccacgag tctcccatga
      301 tgcctgcacg cgcggccaag ggccccgatt accgcgtaat gccgccggaa ccgcggctgc
      361 aaggcgttcc gggccatgat gccgacccgc agcaggatca tcgcgataaa gttcgtgctg
      421 ataatgacgc caacgagaaa tatggctggg tggatcagaa tgccggcatc gcccaaattc
      481 ccgtgaaaga agcgatgaaa atcatcgcgc agaaaggtct tccggcaacg tcaccagcag
      541 caccggagaa gaagtagttc atgaacagag cacgcgccat cgcaaccatc gccctattgc
      601 tgctcagcgg taccattgcg cgcagccaag cggtcccgag caatattggc gtcacctcca
      661 cgaccatgcc tgctcagttg caaaatgtcg gatttgaacc gcaacttaac gcgcaaatgc
      721 ccctcgattt gccgttcgtc gacgaaaacg gcagtaatgt acagctgcgc gattatttca
      781 aacagaagcc ggttgttctg gcgtttgttt actatggctg ccccatgctg tgtaaccagg
      841 tggagcaggg tgtcgttggc tcgctgcgca tgctctcttt tatccccggt cgcgattacg
      901 aagttgtctt cgtcagcttt gatccgcgcg aatctgcgga catggccgcg cagaaaaaga
      961 aatccgctat ggagcatttt aggcgtccag aaactgcctc aggatggcat tttctaaccg
     1021 gtacgaaaga aagcatcgac gtcgccacca aagcggccaa tttccgctac tccttcgaca
     1081 ccaagaataa tctttttgct cacgccagcg gca
//
LOCUS       DATCBB010000207         1406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_331089, whole genome shotgun sequence.
ACCESSION   DATCBB010000207 DATCBB010000000
VERSION     DATCBB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331089"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VII37_02335"
     CDS             <1..681
                     /locus_tag="VII37_02335"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HEY5068587.1"
                     /translation="RAGNRHFVDATVSVARTASLEQVHELSDAIEKRIGEIVPSDVMV
                     HAEPRAPQGEHLFESIRALAQRLGLAIHDVTALQQSGKLFIELHLEVDENLSLRDAHR
                     QATKLEEGIRALRDAPTEVNIHIEPLGTHIATPDAGVGEMKLLAGAVEEFLNSLPSEY
                     DELLNCHDARVRQVEHRILVSCHCTMRSDLPINKIHDVTGALVDRVKEKFPQIHRVTI
                     HPEPFEER"
     gene            complement(678..>1406)
                     /locus_tag="VII37_02340"
     CDS             complement(678..>1406)
                     /locus_tag="VII37_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068588.1"
                     /translation="VTLHEAQLLAPKNSNLPGELVPFGFMAWEKTWLFRLRDCYLVAW
                     KLNDQPINLEDIPARAFDTPEEKERVAALLEPYNDEVTLTPEEDAGFAQLGKERTARH
                     PMRTYVWLPAARAFMLWFSPRIELLPLSGEVFPLADAWENDPVDQAVTAGFVVLNALY
                     VLFGVWGAARLWRQPPAKVAVALIVGYIVVRTAFLATLETPEPRYMLVCFPALLALTA
                     QIFAGKGATEADSTAAENRLQEAR"
BASE COUNT          324 a          426 c          368 g          288 t
ORIGIN      
        1 cgcgctggca atcgtcattt tgtcgatgcc accgtaagcg tggctcgtac tgcaagtctc
       61 gagcaggtcc atgagctgag cgatgccatc gaaaaacgta tcggcgaaat tgtaccttcc
      121 gatgtcatgg tgcacgctga gccacgcgct cctcagggcg agcatctatt cgagtccatc
      181 cgcgcattgg cgcaacgcct cggactagcc atccacgacg taacggctct acagcaaagc
      241 ggcaagctct tcatcgaatt gcatctggag gtcgacgaga atttgtctct gcgtgacgcg
      301 caccggcagg ctactaagct tgaagaaggc attcgtgcat tacgtgacgc gcctacggag
      361 gtcaatattc acatcgagcc gttaggaacg catatcgcga cgccagatgc cggtgtgggt
      421 gaaatgaaac tgctcgcggg agcggtggag gaattcttaa attcgctgcc ttcagaatac
      481 gatgaattgc tgaattgcca tgatgcgcga gtgcgccagg tggagcaccg catcctggtt
      541 tcctgccact gcaccatgcg aagcgatttg cccatcaaca agatccacga tgtcaccggc
      601 gcattggtcg accgcgtgaa agaaaaattc ccgcaaatac atcgcgtgac catccacccc
      661 gagcccttcg aagaacgtta gcgcgcttcc tgaagtcgat tttcagccgc agtggaatcg
      721 gcttctgtcg cgcccttacc agcaaagatt tgcgctgtta gagccagtag cgcaggaaaa
      781 cacaccagca tgtagcgtgg ctcaggtgtc tcgagcgtgg ccagaaacgc tgtgcgcacc
      841 acgatgtaac ccacgattag cgccaccgcg actttcgccg ggggttgcct ccacaagcgc
      901 gctgctcccc acactccaaa caaaacgtac agcgcattca gcactacaaa tcccgctgtg
      961 accgcctggt ccaccggatc gttctcccac gcgtccgcta aagggaacac ttctcccgac
     1021 aatggcaaca actcgatgcg cggagaaaac cacaacataa aagctcgcgc agcgggcagc
     1081 cacacatacg ttcgcatagg atggcgcgcg gtccgctcct tgcctagctg ggcgaatcct
     1141 gcgtcctctt ccggcgtcag tgtcacttcg tcgttgtacg gctcaagtaa cgcggccacg
     1201 cgctcttttt cctctggcgt gtcgaatgcc cgtgcaggaa tatcctccaa attgatcggt
     1261 tgatcattca gtttccaggc caccaggtaa caatcgcgca gccgaaacag ccacgtcttc
     1321 tcccaggcca tgaagccaaa gggcaccaat tcgcccggca gattcgaatt ctttggcgct
     1381 agaagttgcg cttcatgcag ggtaac
//
LOCUS       DATCBB010000208         2518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_233218, whole genome shotgun sequence.
ACCESSION   DATCBB010000208 DATCBB010000000
VERSION     DATCBB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233218"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VII37_02345"
     CDS             <1..412
                     /locus_tag="VII37_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068589.1"
                     /translation="EGFDVSPDKKEIWVANAQDGSISIIDLAAKKVVQTLDANIRGAN
                     RLKFTPDGKLAFVSSLGAPDLVIFDVAARKEMKRVPLGHGAAGILMQPDGQRAFVACT
                     PDDYIAVIDLKSLAVTGKITAAKGPDGLAWAVRK"
     gene            complement(524..1834)
                     /locus_tag="VII37_02350"
     CDS             complement(524..1834)
                     /locus_tag="VII37_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5068590.1"
                     /translation="MNPQWVALLDLLDMNVRYERCEGAELFTENGRRILDFLSGYCVH
                     NTGHNHPHIVRALQQELEARGPVMLQSHVPELAGELAKRLCDLAGGRLQKVFFCSSGS
                     EGIEAAIKFARMSTRRDGLLCAKSAFHGLTAGALSLMSDAFWREGFGPLLADAAVVPF
                     GDLAALEKQLNSKKFAAFVVEPVQSEAGIKMPPPGYLDSAEAICRKTGTLFVLDEVQT
                     GMFRTGPFLAGQHYGVKPDMVVLAKALSGGLAPVGATLMTDAVYEGVYSSLRRAIVHT
                     STFSENSLSMRAGLATLDVLEKESLGMRAAELGEELRQRLREQLAPFEMVKEVRGLGM
                     LSGIEFGTPSKLMLKAAFEVFMKIHPAMFGQIVVMRMFRDKNILTQICGNNFMVLKVA
                     PPLVASRAQLDEFVVAIRDVVELMHTSTSFWTDAVTLAKRAVRI"
     gene            2110..>2518
                     /locus_tag="VII37_02355"
     CDS             2110..>2518
                     /locus_tag="VII37_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY5068591.1"
                     /translation="MNRLDGVDLLRGLAIFFVLMNHVNMRLVIAGVPYTEHLPPQLTT
                     FLFWSGQRGVQIFFAISGFLITSTTLRRWGALSQVSLRGFYLMRFARIVPLLLTLLAV
                     LSVLHLAHLQNFIVPEKTGGLPRALLAALTFHIN"
BASE COUNT          582 a          856 c          530 g          550 t
ORIGIN      
        1 agaaggcttc gacgtctctc ccgacaaaaa agaaatttgg gtagctaatg cgcaggacgg
       61 ctccatctcc atcatcgatc tcgccgccaa aaaagtagtg caaactctgg acgccaatat
      121 tcgcggcgca aaccgcctga agttcacgcc agatggaaaa ttggctttcg tctccagcct
      181 gggcgctccc gatttggtga tttttgatgt agccgcaagg aaggaaatga agcgcgtacc
      241 actgggccac ggcgcagccg gaatcctcat gcagcccgac ggtcaacgcg ccttcgtagc
      301 ctgtacgccc gacgattaca tcgccgtgat cgatctaaaa tccctggccg taaccggcaa
      361 aatcaccgcc gccaaggggc ccgacggcct cgcatgggcc gttcgcaaat aagttttgtc
      421 cttaaggctt attttataaa gccgttaaat cttcacccct cgagggtgcc ccaggttcgc
      481 atttgagtta tgaacctggg tccgaggggc cggtaacaaa ctcctaaatc ctcaccgctc
      541 tcttcgccaa cgtcaccgca tccgtccaga aacttgtcga cgtatgcatc aattccacaa
      601 catcgcgtat cgccaccaca aattcatcca actgcgctcg cgaagccacc agcggcggcg
      661 ccaccttcaa caccataaaa ttattcccac aaatctgcgt cagaatattt ttatcccgaa
      721 acattctcat caccacgatt tgcccaaaca tcgccggatg aatcttcata aacacttcga
      781 acgccgcctt cagcatcaac ttcgacggcg tgccaaactc aatccccgac aacatcccca
      841 atccgcggac ttccttcacc atttcaaacg gtgccagttg ctcccgcaat cgctgccgca
      901 actcctcacc aagctcagcc gcccgcattc ccagcgattc tttctctaat acatccagcg
      961 tggccaatcc tgctcgcatc gacaaactgt tctcactaaa cgtcgaggtg tgtacgatcg
     1021 cgcgccgcag cgacgaataa acgccttcgt aaaccgcgtc cgtcatcaat gtcgcgccaa
     1081 ccggcgccaa acccccgctc aacgccttcg ccagcaccac catatccggc ttaacgccgt
     1141 aatgctgtcc agccaaaaat ggcccagtgc gaaacattcc tgtctgcact tcgtccagca
     1201 caaaaagcgt cccggtcttc cgacaaatcg cctccgcact gtctaaatat ccaggcggcg
     1261 gcattttgat tccggcttcc gactggaccg gctccacaac aaacgccgcg aactttttcg
     1321 aattcagttg cttctccaac gccgccaaat ccccaaacgg aacaaccgcc gcatccgcca
     1381 gcaacggccc aaacccctca cgccagaacg catcgctcat caacgacaac gcgcccgcag
     1441 tcaatccatg aaacgcgctc ttcgcgcaca acaagccatc ccgccgcgtg ctcatgcgcg
     1501 caaatttaat ggccgcttca attccttcgc tcccgctgct gcaaaaaaat actttttgca
     1561 gcctgccccc cgcaagatcg caaagccttt tggccaactc gcccgccagc tccggcacgt
     1621 ggctctgcaa catcaccgga ccgcgcgcct ccaattcctg ctgcagcgcc cgaacaatat
     1681 gcgggtggtt atgcccagtg ttgtgcacgc aatatccgga cagaaagtcc agaatccgcc
     1741 ggccattctc cgtgaacaat tccgcgcctt cgcagcgctc ataccgcaca ttcatatcca
     1801 gcaaatcaag cagcgccacc cattgcggat tcacataatc agaataagaa gttgccgtgg
     1861 aagttgtttt ggaactagct gcggaagttg agctgtgtac ctgcgtgtca atcacagtcg
     1921 tagattttgg catcactaga gagatgccga cattattgcc aaggatgcat taataaaaat
     1981 tcaggtttgt gtgtaatagc tagctgaacc cattcggtac accaggcctt ctctttgtaa
     2041 ggttagcccc gttccctgcc gcataataaa ttcatggccc ggccaccatc tccaataatc
     2101 gcgcccgcgt tgaaccgtct cgacggcgtc gacctgcttc gcggcctggc tatctttttc
     2161 gtcttaatga accacgtcaa catgcgtctg gttattgccg gcgtgcccta caccgaacat
     2221 ctcccccctc agctcacaac ctttcttttc tggagcggtc agcgcggcgt acaaatcttt
     2281 ttcgccatct ccggcttcct cattacctcc acaactctgc gccgctgggg cgcgctctcc
     2341 caagtcagcc tgcgcgggtt ttacctcatg cgcttcgctc gcatcgtccc gctgttgctc
     2401 acgttgttag ctgtgctcag cgtcctgcac ctcgcacacc tccagaattt catcgtcccc
     2461 gaaaaaaccg gcggcctccc tcgggcgctt ctcgccgcac tcaccttcca cattaatg
//
LOCUS       DATCBB010000209         1873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_39530, whole genome shotgun sequence.
ACCESSION   DATCBB010000209 DATCBB010000000
VERSION     DATCBB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39530"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VII37_02360"
     CDS             complement(<1..578)
                     /locus_tag="VII37_02360"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068592.1"
                     /translation="MARVSPQELLSRLEKGKVIPAILLLGDEPYLRDACRAQLIEAFV
                     PEASRTWAVSRYSADRGEIEAALSQAQSLPMLSPQQVIFLEDAEAIEKLGDKNREETV
                     ALLELYLGDPAPFTVLVIEAARLDERMKLAKILAQKMLVVEVGLGENPEQRQSAAVLM
                     AQNFAKTLDLQFEKGAAEDLAERVSADLIRLKT"
     gene            complement(594..1127)
                     /gene="lptE"
                     /locus_tag="VII37_02365"
     CDS             complement(594..1127)
                     /gene="lptE"
                     /locus_tag="VII37_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY5068593.1"
                     /translation="MGSKLAWRALLFMLLMATLQTGCGYHVAGHSNSLPKSIHVIAVT
                     ALENKTTSYRIEQKLTSATVHEFLARTPYKIVSNAANGDAVLRGTVVSVEAVPLLFDT
                     QTGRATTMLITVKCEVSLTESATDKVLYHSENFVFRNEYEISTDVPSFFEEQDPALDR
                     LAQDFARKLVAAVIENY"
     gene            complement(1127..>1873)
                     /locus_tag="VII37_02370"
     CDS             complement(1127..>1873)
                     /locus_tag="VII37_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEY5068594.1"
                     /translation="PAALNTGAKIIDLSGGAAEREDAVAWFPKLDWLRGREFVTGAKI
                     FAVPCAFATAAAGLALSLSRVGLRRIMLIGFEPVSEAGPAGIEELESQTTQLLSFQGL
                     GQPVFDTQVACNLLDRFGPASSHKLCATRERVRAETKACINGKCAMPAIQVVHAPVFY
                     GSVFSATGVFDRGSSAENISNACGDAGFSIPPDPNIGPSNISVSGLNSIQLSTPEPDA
                     SNPETWWFWGAADNMHFPAANAVKLAEMLL"
BASE COUNT          425 a          561 c          476 g          411 t
ORIGIN      
        1 gttttaagcc gtatcaaatc cgccgaaacg cgctccgcga gatcttcggc cgcgcctttt
       61 tcaaattgca gatcaagtgt cttcgcaaaa ttctgcgcca tgagcacagc ggccgattgc
      121 cgttgctcgg ggttttcgcc caagccgact tccaccacca gcattttctg cgcaagtatt
      181 tttgccaact tcatacgctc gtcgagcctg gccgcttcaa tcaccagcac cgtgaagggc
      241 gccggatcac ccagatatag ttccagtagg gcgacggtct cttcgcgatt cttgtctccc
      301 agcttctcga tcgcttcggc atcctccaga aaaatcacct gctgtggcga cagcatcggc
      361 aaactttgcg cttgcgacag cgcggcctcg atctctccgc gatccgccga ataacgagac
      421 accgcccagg tcctggacgc ttccggcaca aatgcttcaa tcagttgcgc gcggcaagcg
      481 tcccgcaggt acggctcatc gccaagtaaa agtattgccg gaatgacttt ccctttttca
      541 agccggctca ataattcctg cggtgaaact ctggccatta aaacgctccc ggactaataa
      601 ttctcaatga ccgccgccac aagcttcctg gcaaaatcct gcgccaagcg atccagcgcg
      661 gggtcctgct cttcgaaaaa actcggcaca tccgtggaga tttcatactc attgcggaaa
      721 acaaaattct cactgtgata tagcactttg tcggtggcac tttccgtcag tgagacttcg
      781 catttgacgg taatcagcat ggtggtggca cgtcccgtct gggtatcaaa aagcaatggc
      841 acggcttcca cgctcaccac cgtaccgcgc aacaccgcgt ctccattcgc ggcgttcgaa
      901 actattttgt atggcgtgcg agccaggaat tcgtgcaccg tcgcggacgt aagcttttgc
      961 tcgatgcggt agctggtggt cttgttttcc agcgctgtca cagcaatcac gtgaatgctt
     1021 ttcggcaaag aattgctatg ccctgcgacg tgatagccgc atccagtctg aagtgttgcc
     1081 atcagcaaca tgaacagtaa agctcgccac gccagcttcg aacccattac aaaagcatct
     1141 ccgcaagctt cacggcgttg gcggccggaa aatgcatgtt gtctgcggcg ccccagaacc
     1201 accaagtctc cgggttagat gcgtctggtt ccggtgtaga aagctggatt gaatttaagc
     1261 ccgagacact aatattgctg ggcccaatgt tgggatccgg cggaatagaa aagcctgcat
     1321 ccccgcaggc gttggaaatg ttttccgcgg agctgccgcg gtcgaaaacc cccgtggcag
     1381 agaaaacgga accgtaaaat accggggcgt gcacgacctg gattgcaggc atggcgcatt
     1441 ttccatttat gcacgctttc gtctcggcac gcacccgttc gcgagttgcg cataacttgt
     1501 gagagctcgc cggaccaaag cgatcgagca ggttacaagc gacctgcgta tcaaaaaccg
     1561 gttgacccag cccttgaaac gaaagtagct gcgtcgtttg actttcgagt tcctcaattc
     1621 cagccggacc cgcttccgag acgggttcaa atccgattaa catgattcgc cgcaggccga
     1681 cgcgcgaaag cgacaaagcc agtcccgcgg ccgccgtcgc aaaggcgcaa gggacggcaa
     1741 aaatttttgc gccggtaacg aactcgcggc cacgcagcca gtcaagttta gggaaccatg
     1801 ccaccgcgtc ttctcgctcg gcagcccctc ccgacaaatc aattattttc gctccggtat
     1861 tcaaagcggc ggg
//
LOCUS       DATCBB010000210         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_233282, whole genome shotgun sequence.
ACCESSION   DATCBB010000210 DATCBB010000000
VERSION     DATCBB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233282"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VII37_02375"
     CDS             complement(<1..642)
                     /locus_tag="VII37_02375"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5068595.1"
                     /translation="MATAELKKGDVKDIGLADAGKRKIEWAQQQMPVLESIRKRFLKE
                     QPLKGLRVSACLHVTSETANLMIALRDGGADAVLCASNPLSTQDEVAASLNRDYNIPT
                     YAIKGEDNETYYTHLRAALDHKPQFTMDDGADLVTMLLTKRTDLVPNVIAGTEETTTG
                     VIRLRAMAKDGTLKYPIVAVNDAMTKHFFDNRYGTGQSTIDGVIRATNVLIAGL"
     gene            complement(723..1925)
                     /gene="metK"
                     /locus_tag="VII37_02380"
     CDS             complement(723..1925)
                     /gene="metK"
                     /locus_tag="VII37_02380"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY5068596.1"
                     /translation="MSEALRNFLFTSESVTEGHPDKIADQISDAVLDEVMRQDPMGRV
                     ACETLVTTGLAVVAGEITTTAHVNYDELVRETIRGVGYDRAKYGYDAETCAVMCTVKR
                     QSPDIAMGVDTGGAGDQGLMFGFACDETEELMPMPIQLAHRLTQRLADIRKQKKVNFL
                     RPDGKSQVTIEYRNGRPARIEAVVISTQHDDKVSNEELREVISTQVIKPIIPAAMLDS
                     KTKYHINPTGRFVIGGPMGDAGVTGRKIIVDSYGGYSRHGGGAFSGKDPSKVDRSACY
                     MARYIAKNIVAAGLASKAEVQLAYAIGVAEPVSVMVDAFGTETVAEEKITKLVREFFS
                     LTPKGIIEALDLRRPIYKPTAAYGHFGRTGPGFTWERTDRASALREAAGLGASNKASA
                     TAEVGARR"
     gene            complement(2125..3225)
                     /gene="prfB"
                     /locus_tag="VII37_02385"
     CDS             complement(2125..3225)
                     /gene="prfB"
                     /locus_tag="VII37_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY5068597.1"
                     /translation="MCGVIFDAPQHRERLKSLEARVADPNFWSNQEAAQAVLRDRKRA
                     EDQVGADDKLASMSEDIETYINLAQEETNAEQRNDLLKDLDRELDAAETYVAELETKT
                     LLSGENDHLNAIMTIKPGAGGTESQDWASILLRMYLRWAERKGMGAMVLDETPGEEAG
                     IKSATIQFTGENAYGLLTGETGVHRLVRISPFDQAARRHTSFASVFVIPEIDDRIEID
                     VKPEEIRVDTFRAGGAGGQNVNKVETAVRITHLATNIVVQCQAERSQHKNREIAMKMM
                     RSRLYDLEIQKRQAATDKLDESKLDISFGSQIRSYVMQPYRLIKDHRTKFEVGDVDRV
                     LDGDLDPFIRSYLMAKKTKGKLTVQLEDADVA"
     gene            complement(3277..>4158)
                     /gene="lnt"
                     /locus_tag="VII37_02390"
     CDS             complement(3277..>4158)
                     /gene="lnt"
                     /locus_tag="VII37_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY5068598.1"
                     /translation="ARLSLAHREHEVDLLVWPEAPAPFSYQDPRFAQFASQLAVKFGY
                     PFLVGVIEWKPPADGAATVAHAAMVPFNSALLFNAQGQRIFSYDKIHLVPFGEYEPFP
                     LIHEVVNRVSDEVGGFRKGQTYGVGPLPGGYSLGVFICYEAIYPGEVRRFAADGATLL
                     VNISNDGWFGRSEAARQHLLMARVRAVENRRWLLRVTNNGYTASVDPYGRMYEPLPTD
                     VRASADLPYDFRTDRTIYTRFGDWFAWLCIAVSVILLSSTFWKGNVSRDSIPIPVDFL
                     ASPIRVVPDAVTSRSST"
BASE COUNT          934 a         1279 c         1005 g          940 t
ORIGIN      
        1 caagcccgcg atcagcacat tcgtggcgcg aatcacaccg tcaatcgtcg attgtcccgt
       61 gccgtagcga ttgtcgaaga agtgcttggt catggcatcg ttcaccgcca cgatcggata
      121 cttcagcgtt ccatccttag ccatggcccg caaacgaatt acaccggtag tagtctcttc
      181 cgttccggca atcacgttcg gcaccaaatc agtccgcttg gtcaaaagca tggtgaccag
      241 gtccgcgcca tcatccatcg tgaattgcgg cttatgatcg agcgccgcgc gcaaatgcgt
      301 gtaataggtc tcgttgtcct cgcccttgat ggcatatgtc gggatgttgt aatcgcgatt
      361 cagcgacgcc gccacttcgt cctgcgtcga aagcggattc gacgcgcaca gcaccgcatc
      421 cgccccgcca tcgcgcaaag caatcatcag gttcgcggtc tcgctggtga cgtgcaggca
      481 agccgaaacg cgcaaacctt tcagcggctg ctccttcaaa aatcgcttgc ggatcgattc
      541 caacaccggc atctgctgct gcgcccactc aatcttgcgt ttacctgcgt cagccagccc
      601 gatatctttc acatcacctt ttttcagttc agcggtagcc aatgaaatgc tccttaatta
      661 aattttgccg gcgaaaattt cgctcaagga aagggaaata aaaattcttc aattatttaa
      721 aactagcgac gtgctccaac ctcagcggtc gcactcgcct tattcgacgc accaagtccg
      781 gcagcctcgc gcaaagcgct ggcgcgatca gtacgctccc aggtaaatcc aggcccagtg
      841 cgcccaaagt gcccgtaagc cgcagtcggc ttgtaaatcg gccgccgcag atccagcgct
      901 tcaataatgc ccttcggcgt taacgagaaa aattcgcgaa cgagcttggt gattttttct
      961 tccgccaccg tttccgtgcc aaaggcatca accatcaccg agactggctc agctacgccg
     1021 atcgcatacg ccaactgcac ttccgctttg cttgccaatc cggccgccac gatattcttc
     1081 gcaatgtaac gcgccatgta gcacgccgag cgatccacct tgctgggatc cttgccggaa
     1141 aaagctcctc caccgtgccg gctgtagccg ccgtacgagt ccacgataat ttttcgtccg
     1201 gtcacaccgg catcgcccat cgggccgccg atcacgaatc gcccagtcgg attgatgtga
     1261 tatttcgttt tggaatccag catcgccgcg ggaataatgg gcttgatgac ttgcgtagaa
     1321 attacttcgc gaagctcttc gttcgaaacc ttatcatcgt gctgcgtgga gatcaccacc
     1381 gcttcgatgc gtgccgggcg gccattacga tattcaatgg taacttgcga ttttccatcc
     1441 ggccgcagga aatttacttt cttttgcttc cgaatatccg ccagccgttg cgtcaagcga
     1501 tgcgccagtt gaatcggcat gggcatcaac tcttccgtct cgtcgcacgc aaaaccaaac
     1561 atcaagccct ggtcgcccgc tccgcccgta tccacgccca tggcgatatc cggcgactgg
     1621 cgcttcaccg tgcacatcac cgcgcaagtt tccgcgtcat agccatactt cgcgcggtcg
     1681 taacccaccc cgcggatggt ctcccgcacc agttcgtcat aattcacatg cgcggtggtg
     1741 gtaatttctc cggcaaccac ggccaaaccg gtggtcacta gcgtctcgca ggccacacga
     1801 cccatcgggt cttgccgcat cacttcatcc agcaccgcat ctgaaatttg gtccgcgatt
     1861 ttatccggat ggccttccgt cacggattcg gatgtaaata ggaaattgcg taaggcttcg
     1921 ctcaaaacaa atctccttag tgtgtctgcc ggggacggca gtcaggcggc gcaaacagcg
     1981 cggcttctaa attatagtag gtactcttga acggtaccat tcgaaaacat aagaatcaaa
     2041 ttactgaatc caaatgcgtt agaagccacg tgaacggtaa caggagaggg ttgagtggat
     2101 gaagtaccta tcattggaaa gctcttaggc cacgtctgcg tcctccaact gcacggtcaa
     2161 cttccctttc gtcttcttgg ccatcaagta agaccggata aacgggtcca agtccccgtc
     2221 cagaacgcgg tccacgtccc caacttcaaa tttcgtgcgg tgatccttga tcagccggta
     2281 cggctgcatt acatacgacc gtatctggct gccaaacgaa atatccagct tcgactcgtc
     2341 cagcttatcg gtagcggcct gccgcttttg aatttccagg tcgtatagcc gcgagcgcat
     2401 catcttcatc gcaatctcac gatttttgtg ttggctgcgc tccgcctggc actgcaccac
     2461 aatgttcgtg gccaggtgcg taatccgcac cgcggtttcc actttattta cgttctgtcc
     2521 gccagcgccg cccgcgcgaa aggtatccac gcgaatttct tccggcttca catcaatttc
     2581 aatgcgatca tcaatttccg gaatcacaaa caccgatgca aacgaagtat gccgccgcgc
     2641 cgcttgatcg aacggtgaaa tgcgcaccag ccggtgcacc cccgtttcgc cagtcagcag
     2701 tccgtaagca ttttcgccgg tgaattgaat tgtcgcggac ttgatgcccg cttcttcgcc
     2761 cggcgtttca tccagcacca tcgcgcccat gcccttgcgt tccgcccaac gcaaatacat
     2821 gcgcagcaaa atggacgccc agtcctgcga ctctgtgccg cccgcgcccg gcttgatggt
     2881 catgatcgca ttcaggtgat cgttctcacc cgaaagcaaa gtcttcgttt caagttccgc
     2941 gacatacgtc tccgccgcat ccagctcgcg atccagatcc ttcagcaaat cgttgcgctg
     3001 ctccgcgttg gtttcttctt gcgccaaatt gatgtacgtc tcgatgtctt cagacatcga
     3061 cgcaagtttg tcatccgcgc ccacctggtc ctcagcgcgc ttgcggtcac gcagcaccgc
     3121 ctgagcggct tcttgattgc tccagaaatt ggggtccgcg acgcgcgctt cgagggattt
     3181 caggcgctcg cgatgctgcg gggcgtcaaa gataactccg cacaagcgtg atgcgctgtt
     3241 tcagcccttc gtacttgtgc tgcaaatctt caataatcat gtggaagaac gcgatgtgac
     3301 cgcatcggga actactcgaa tcggcgatgc cagaaaatct accggtatcg gaatactatc
     3361 gcgcgaaaca tttcctttcc agaaagttga ggacaacaga ataacggaaa ccgctatgca
     3421 gagccaagcg aaccaatctc caaatcgcgt gtaaatggtg cgatcggtgc gaaaatcgta
     3481 tggcagatcc gcgctcgcgc gcacatccgt aggcaacggc tcgtacattc gcccgtaagg
     3541 atcgaccgag gcggtgtatc cattgttcgt cacacgcagc agccatcggc gattctccac
     3601 tgcgcgaaca cgtgccatca ataaatgctg tcgcgcagcc tcggaacgcc caaaccaacc
     3661 gtcgttcgaa atattcacca gcaatgttgc gccgtcagcg gcgaagcgtc tgacttctcc
     3721 ggggtaaatc gcctcgtaac aaataaatac tcccagagaa taaccgcccg gcaagggacc
     3781 tactccatac gtttgacctt tgcgaaatcc gcccacttca tccgacacgc gattcacaac
     3841 ttcatgaatc aacgggaatg gttcgtattc gccaaagggc accaggtgga ttttgtcgta
     3901 agaaaaaatc ctctgccctt gcgcattgaa aagtagcgcg ctgttaaagg gcaccatcgc
     3961 ggcatgcgca acagttgcag ctccatccgc gggaggcttc cattcgatga cgcccaccaa
     4021 gaatggataa ccgaatttca ccgcgagctg gctcgcaaat tgcgcaaatc ttggatcttg
     4081 atacgaaaat ggtgccggcg cctccggcca aaccagtaaa tccacctcgt gctcgcggtg
     4141 cgcaagactc aagcgcgc
//
LOCUS       DATCBB010000211         1193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_266485, whole genome shotgun sequence.
ACCESSION   DATCBB010000211 DATCBB010000000
VERSION     DATCBB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266485"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VII37_02395"
     CDS             complement(<1..429)
                     /locus_tag="VII37_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HEY5068599.1"
                     /translation="MSIAREQEKCRSARFVDDDRFKIFSGTAHVPLAESICRNLNVTI
                     GKALLGRFSDGEIYFQILENVRGADVFVVQPCCYPVDNHLLELLLMIDAFKRASAWRI
                     TAVLPYYCYARQDRKDKPRVPISAKLVADLLETAGASRALT"
     gene            complement(568..>1193)
                     /locus_tag="VII37_02400"
     CDS             complement(568..>1193)
                     /locus_tag="VII37_02400"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068600.1"
                     /translation="GGGLGGGSSNAAAAIFGYLQLVGRKLPMAVLLKLAASLGADVPI
                     FLFGGRALGAGRGDEIYPLPDIKKLALLIVAPRDIRVPTPDAFRWLNAPLRNAATLTK
                     LAADPKLWEFCALSWGAQGNGLSNDFEKPVFKRYNRLDQIKRVLLQKGAAEASLAGSG
                     SAVFGVFPSPTMARRAAVGFPHDLTFVCETISRDRYLRAVKSARVVR"
BASE COUNT          269 a          387 c          313 g          224 t
ORIGIN      
        1 cgtcagcgcc cggctggctc ccgccgtttc cagcaagtcc gccaccagct tcgcggaaat
       61 cggtacccgc ggcttgtcct tgcggtcttg ccgcgcgtag cagtaatacg gcagcaccgc
      121 ggtaattctc cacgccgacg cccgcttgaa ggcgtcaatc atcagcagca actccagcag
      181 atggttgtcc accggatagc agcacggctg caccacgaaa acatccgcgc cgcgcacgtt
      241 ttccagtatt tgaaaataaa tctcgccgtc gctgaaacgc cccagcaacg ccttgccgat
      301 cgtcacattc aagttgcggc aaatactttc ggccaacggc acgtgcgccg tgcccgaaaa
      361 tattttgaag cggtcgtcat ccacgaagcg cgcgctcctg catttctctt gctctctagc
      421 gatggacacc gcgggtgccc cgggttcgca gttgaggtat gaacccgggt cttgccgtgc
      481 gatcgtgtcc gatcaatcgg atcaaccaaa aatcacttgc gcgaactgcc ttacattggt
      541 ttcgatggat acggcggacg ctgcggctca acgaacgaca cgcgcgcttt tcacagcgcg
      601 gagatacctg tcgcgcgaaa ttgtttcgca aacgaaggtc aagtcgtgcg gaaacccaac
      661 agcggctcgg cgcgccatcg ttgggctcgg gaaaactcca aacaccgccg aaccgctacc
      721 cgccaacgag gcttctgcag ctcccttttg aagcaaaacc cgcttgattt gatccagccg
      781 gttatagcgc ttgaaaacgg gcttctcaaa atcgttagaa aggccgttcc cctgcgcgcc
      841 ccaggacaga gcacagaact cccaaagttt agggtctgca gcgagttttg tcaacgtagc
      901 cgcgttgcgc aaaggggcat tcagccagcg aaacgcgtcg ggcgtaggca cccgaatatc
      961 gcgcggagcg acaatcagca gcgccaattt cttaatgtcg ggcaacggat aaatctcatc
     1021 gcccctgccc gcccccaggg cccgaccgcc aaataaaaat ataggcacat cggcgcctag
     1081 tgaagccgcc agtttcaaca aaaccgccat gggcagcttg cgcccaacca gttgaaggta
     1141 tccgaatatt gccgctgccg cgttgctaga gccgccgccc aacccgccgc cag
//
LOCUS       DATCBB010000212         1247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_201457, whole genome shotgun sequence.
ACCESSION   DATCBB010000212 DATCBB010000000
VERSION     DATCBB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201457"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VII37_02405"
     CDS             complement(<1..866)
                     /locus_tag="VII37_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEY5068601.1"
                     /translation="MKTNPHFYPVILAGGRGTRFWPLSRKRLAKQLLALDGKQTMIQQ
                     TVARLTPMAAPSRFWIITNDDLRTAIQRQLPKLPSKQIIAEPAARNTAPAIGLAAFLL
                     LRRDPEAVLGLFPSDHVIADSNQYRAILSRGIEIAAAGDNIVVLGIRPTRPETGYGYI
                     ESGAPAHDGSLRVRRFTEKPDLTRARQFVEAGNFFWNSGMFLWRADTLANALREHLPN
                     TAPLLEKIAAAYGTSKFAETFRKLYPKCENISIDYAVLEPRSSKGEAESRIFCLPSDF
                     GWNDLGSWTALH"
     gene            complement(955..>1247)
                     /locus_tag="VII37_02410"
     CDS             complement(955..>1247)
                     /locus_tag="VII37_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068602.1"
                     /translation="APYFTVDMFELKEPHVFSTAASGKDNSGKTSVQILVAVEGCGVV
                     EAQGRDPVTLAKGDAVVVPSAVQSFTVRPQWAVEFLKASVPGGVVPEPATRM"
BASE COUNT          269 a          352 c          377 g          249 t
ORIGIN      
        1 tcgtgtagcg cagtccacga gccgaggtca ttccatccga agtcggaggg caggcagaag
       61 atgcgcgatt cagcttcccc cttcgacgac ctcggttcga gaaccgcgta atcgatgctg
      121 atgttttcgc actttggata aagcttccga aaagtttctg cgaacttgct ggtgccgtag
      181 gctgccgcaa ttttttccag gagcggcgcg gtgttgggca gatgctcacg caaagcattg
      241 gccagcgtgt cagcacgcca gaggaacatt ccactattcc aaaagaagtt gcccgcctcc
      301 acaaactgac gcgcccgggt caagtctggc ttctcggtaa accggcgcac gcgcagagag
      361 ccgtcgtgag ccggagcacc cgactctata tagccatagc ccgtttcggg gcgagtgggg
      421 cggatgccga ggacaacgat gttatcgcca gcggcagcaa tttcgattcc ccggctaagg
      481 atggcgcggt actgattcga gtcggcaata acgtggtcag acggaaacaa gcccagcacg
      541 gcctcaggat cgcgacgaag tagaagaaat gccgcaagcc cgatcgcggg agccgtgttc
      601 cgcgcggcgg gttcggcaat gatctgcttc gatggaagct tcggaagctg acgttggatg
      661 gctgtgcgaa gatcgtcgtt cgtgatgatc cagaaacggc tcggggccgc catgggagta
      721 agccgcgcga ccgtttgctg gatcatggtc tgcttgccat ccagcgcgag cagctgcttg
      781 gcgaggcgtt tgcggctcaa aggccagaag cgcgtgcctc ggccaccagc caaaattacg
      841 ggataaaagt gcggattagt cttcatctta aggtcttaga aagcctacca tttttgaggc
      901 ggtgcgaggc gaagtgaccg ggtggtgatg gtaaatgcgg gaactggcaa agttttacat
      961 gcgcgtcgca ggttcaggca cgactccccc cggtacgctc gctttcagga actcaactgc
     1021 ccactgcggg cgcaccgtga aactttgcac agcagacggg actaccaccg cgtcgccctt
     1081 agcgagcgtt accggatcgc gtccctgcgc ctcgactact ccgcaaccct cgacggcgac
     1141 cagtatttga accgaagttt ttcccgaatt gtccttcccg gacgcggcgg tcgaaaaaac
     1201 gtgcggctcc ttcaattcaa acatgtccac cgtgaaatag ggtgcgg
//
LOCUS       DATCBB010000213         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_201483, whole genome shotgun sequence.
ACCESSION   DATCBB010000213 DATCBB010000000
VERSION     DATCBB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201483"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..418
                     /locus_tag="VII37_02415"
     CDS             50..418
                     /locus_tag="VII37_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5068603.1"
                     /translation="MSNPALKYLAWKDVEREQLNPHIDREMVVGDKIMLARVLMKKGA
                     HVPLHHHHNEQVTYILEGVLKFNVGGKEIVVRAGEVLCIPPNMPHEAWAEEDTVDLDV
                     FNPPREDWLNKTDDYLRHGR"
     gene            575..1351
                     /locus_tag="VII37_02420"
     CDS             575..1351
                     /locus_tag="VII37_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068604.1"
                     /translation="MNKLLLSVLLGCAVVGGARAQENVYSTPTAGSNAAANFSATSAA
                     RVPLVQPLAQAGPLFTADALDLRFSSTPEPATVTTALSLPLAAPNAAAPEPSPAPSPR
                     FIFSDRDDFRWQLGLGLSFERFRSDIYSASAVGTTTDLTYFLSDFLGIEGDVNTFFAP
                     TIWQNEHIKLVNYGAGPKFEWRRPRWEPWFHGLFGGTHAFPQTAGHSKNGFAVQLGGG
                     ADYRLLPRLSARAEVDYVGTRLFGQWQNSGQATLSLVFHF"
     gene            complement(1431..>2346)
                     /locus_tag="VII37_02425"
     CDS             complement(1431..>2346)
                     /locus_tag="VII37_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068605.1"
                     /translation="VCLAASAVITDASEYRNRNSVSNARRDANTNAAEVDDVLAPAFT
                     AIPELTSGFQLLYAQQFPEAREKFMTWESEHPEEPFGEVAIAASYLFEEFYRQGVLTS
                     DFFLNEEKFLNGIDGKPDPGHMAKFQDALVKTRALANKRLKKKPKDTEALFALTLTAG
                     MESDADMILRKERLEALKLLKLANQYAKQLLAVRPDANDAYVALGSANYIIGSLSGGM
                     RFFLSFGGVHGDKKLGMQQLGKSADSGLYLRPFAKIMLALAARREKQDALAQKLLREL
                     SEEFSSSPLFAAEYAKSMGRPVPAVMHP"
BASE COUNT          524 a          635 c          633 g          554 t
ORIGIN      
        1 cgcatctgta tctgcacgac aatgccgcga aaatttatgg agaaaagaaa tgagcaatcc
       61 agcgttgaaa tatcttgcat ggaaagacgt ggaacgcgag caactaaatc cccatatcga
      121 tcgcgaaatg gtggtcgggg acaaaatcat gttggcgcga gtgctcatga agaaaggcgc
      181 gcacgttccg ctgcatcacc atcacaatga acaagtcacc tatattctgg aaggcgtgct
      241 taaattcaac gttggaggta aggaaatcgt agtgcgcgcc ggcgaggttc tgtgcattcc
      301 gccaaatatg cctcatgaag cgtgggccga ggaagacacc gtcgatctgg atgtcttcaa
      361 tccaccgcgc gaggactggc tgaacaaaac cgatgactac ctgaggcacg gccgttaagt
      421 gctaaaccac gttcagtctc attcgtccgg gaatgccggc cgggcgcgag gcaccacaaa
      481 tgttagttaa acccgtagac aaaagggctc agtcttgttt accgtagtga gtggttgatt
      541 ccaggcttgg ggtcagcccc tcaaggggaa catcatgaac aagctattgc tgtcagtgtt
      601 attaggttgc gcggtcgttg gtggcgcccg ggcgcaggaa aatgtttaca gtacacccac
      661 tgcgggttcg aatgcggcgg ccaattttag cgcaacaagc gcggcccgcg tgccgctcgt
      721 acaacccttg gcgcaagctg gcccgctctt taccgctgat gcactggact tgcgattttc
      781 aagcacgcct gagccggcca cggtaactac cgcactgtca cttcctttgg cggccccgaa
      841 tgccgcggcg cccgagcctt cgcccgcacc ttctcccaga tttatcttta gcgatcgcga
      901 tgatttccgc tggcaacttg ggcttgggct ttctttcgag cgtttcagat ctgacattta
      961 tagcgccagc gcggtcggca ccaccacgga tctcacttat tttctaagtg attttcttgg
     1021 aattgagggc gatgtgaata ctttttttgc ccctaccatc tggcagaacg aacatatcaa
     1081 gcttgttaat tacggcgctg gtccaaaatt cgagtggcgg cggcctcgct gggaaccttg
     1141 gtttcacgga cttttcggcg gcacgcacgc attcccgcaa accgccggtc acagtaaaaa
     1201 tggatttgcc gtgcaattgg gcggaggggc ggactatcgc ttgctgccac gtctttcggc
     1261 cagggctgaa gtcgactatg tcggtacccg actttttgga cagtggcaaa atagcggcca
     1321 ggcgactctg agcctggtct ttcacttctg acggtcagct tatgcgattg attttgccga
     1381 acgatgggct tgcgctagca accgtaccga ctccactttg acgttgcgaa tcagggatgc
     1441 ataacggccg gcaccggccg tcccatggat ttcgcatact ccgcggcaaa tagcgggctg
     1501 gaagaaaact cctcactcaa ttcccgtaaa agtttttgcg ccaaagcatc ctgtttctct
     1561 cgccgtgccg ccagtgccaa cataattttg gcgaagggcc gcagatacaa gccggagtcc
     1621 gccgatttcc ccaactgttg catgcccagc tttttatccc catgtacgcc accaaacgac
     1681 aggaagaaac gcatgccgcc cgaaaggctt ccaataatat aatttgcgga gcccaaggcg
     1741 acgtacgcat cgttcgcatc tggcctgacc gccagcaatt gcttggcgta ctggttcgct
     1801 aatttaagca gcttgagtgc ttcgaggcgt tctttccgca ggatcatgtc tgcgtccgat
     1861 tccatgccgg cggtgagagt tagggcaaaa agcgcctcag tgtctttggg ctttttttta
     1921 aggcgcttat tcgccagggc tcgtgttttt accaaggcgt cctgaaattt ggccatatgg
     1981 ccggggtcgg gcttgccgtc tatgccgttc aggaattttt cttcattcag gaaaaaatcg
     2041 ctggtcaaaa ctccctggcg gtaaaactcc tcgaaaaggt agcttgcagc gatggctact
     2101 tcaccaaaag gttcctcagg atgttccgat tcccaggtca taaatttttc gcgcgcttcg
     2161 ggaaactgct gcgcgtagag cagttgaaat ccagaagtga gctctggaat ggccgtgaac
     2221 gccggcgcaa gtacatcatc gacctcggcg gcgttggtgt tagcatcgcg gcgggcattg
     2281 gagaccgagt tacgattgcg atactcactc gcatcggtga taacggcgct cgccgccaaa
     2341 cacacc
//
LOCUS       DATCBB010000214         1121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_39645, whole genome shotgun sequence.
ACCESSION   DATCBB010000214 DATCBB010000000
VERSION     DATCBB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39645"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VII37_02430"
     CDS             <1..201
                     /locus_tag="VII37_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068606.1"
                     /translation="LLAAFALFAAGFAVIGLGIAALYLMQKAWEVGVARFADSQSPAA
                     SMARRAASAMEELGRRPFRRPV"
     gene            complement(371..>1121)
                     /locus_tag="VII37_02435"
     CDS             complement(371..>1121)
                     /locus_tag="VII37_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068607.1"
                     /translation="PDIWVYDIERGALRRITFAPGEDELPVWSPDGKRIAFASNGRQQ
                     AFMVAVDGSGQEEPLMKNESHFHLQSWLPNGKLIAYERVGNSGQYEIWMLPMEGDRKP
                     YPYVVSQFRVDQPAFSVDGNWLAYTSNESGRSEVYAQRFPGPGEKVQVSTDGGSNPVW
                     SRDGKQLIFENAGTLWATEVLVSPFRVGKSRVLYQGDIWDDAAGPNYALAPGGKKIVV
                     VQRIKDPDGGNLKVVVNWDEELQNLAGTDNK"
BASE COUNT          214 a          334 c          319 g          254 t
ORIGIN      
        1 ctactggctg ccttcgcgct gtttgccgcc ggattcgctg tgatcgggct gggcatcgcc
       61 gcactgtact tgatgcaaaa ggcctgggaa gttggagtgg cgcggttcgc cgacagccag
      121 agcccggccg ccagcatggc gcgtcgagcg gcatcggcaa tggaagaatt gggccgccgt
      181 ccgtttcggc gccccgttta gctcgcggga cgaacgatcg agtttttcag cggcagacaa
      241 tttctggttt tagccagaac accacggagc gagttttcag tcttccttcg tgaaaaagac
      301 caaccgtcct gagagagcgg ttggtctttt aatttttctg gaatgttttt cgcgccggaa
      361 tttttccggc ttatttgttg tcggtgccgg ccaggttctg cagctcttca tcccaattca
      421 cgaccacttt cagattgccg ccgtcggggt ccttgatgcg ctgcacgaca acaatttttt
      481 tgccacccgg cgcaagcgcg tagttcgggc cggcggcatc gtcccagatg tcgccctgat
      541 aaagcacgcg cgatttgccg acgcggaagg gcgacaccaa gacttccgtg gcccacagcg
      601 ttccggcatt ttcaaagatc agctgcttgc cgtctcgcga ccataccgga ttgctgccgc
      661 catcggtgga gacttgtacc ttctcgcccg gcccgggaaa gcgctgcgcg taaacttcgc
      721 tgcggcccga ctcgttcgag gtataggcca accagttgcc gtccacggaa aaggcgggct
      781 gatccacacg gaattgtgaa accacatacg gatacggttt gcgatcgcct tccatcggca
      841 gcatccagat ttcgtattgt ccggaatttc ccacgcgctc gtaggcgatc agctttccgt
      901 tgggcaacca cgattgcaaa tggaaatgcg actcgttttt catcagcggt tcttcctggc
      961 cgctgccatc cacggcaacc atgaaagcct gctgccggcc attggaagcg aaagcgatgc
     1021 gctttccatc cggagaccac acgggaagtt cgtcttcacc cggcgcaaaa gtaatgcggc
     1081 gcaaggcccc gcgctcgatg tcgtataccc agatgtcggg a
//
LOCUS       DATCBB010000215         1297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_233443, whole genome shotgun sequence.
ACCESSION   DATCBB010000215 DATCBB010000000
VERSION     DATCBB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233443"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VII37_02440"
     CDS             complement(<1..421)
                     /locus_tag="VII37_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5068608.1"
                     /translation="MPFVETKDLRMHYQLDGSADAPTLVLSNSLGTNLSLWDSQLPIF
                     SQRFRVLRYDSRGHGQTSATQGEYSVEMLGRDVLQLLDALNLQRVNFCGLSIGGMTGM
                     WLAVNAPQRLEKLVLSNTAPKIGKPDTWNERIRAVREG"
     gene            complement(433..>1297)
                     /locus_tag="VII37_02445"
     CDS             complement(433..>1297)
                     /locus_tag="VII37_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY5068609.1"
                     /translation="KFAGWLDVLGRHRERFREVQNRCLVLQFGGAAGTLAALGSRGGA
                     VARNLSEHLKLPLPQLPWHTHRDRLAEIATTLGLLTGTLGKIARDISLHMQTEIDELR
                     EPAEEGRGGSSTMPHKRNPVACAMILAAAIRMPGLVSIMLSAMIQEDERGLGGWHAEW
                     ETLPEIVCLTAAALRQLTTLIPRLEIDVQRMRENLELTRGLIFAEAITAALGEKIDRS
                     QARELIEAASERATKEKRHLRDVISDDQKIAKHLSSDQLDKLFDPRNYTGIASEFIDA
                     AIKNYKSHKNS"
BASE COUNT          275 a          345 c          356 g          321 t
ORIGIN      
        1 caccctcgcg aactgctctt atgcgctcgt tccaggtgtc gggctttcca atttttggcg
       61 cggtgttgga gagcaccagc ttttcgagcc gttgtggcgc atttactgcc agccacatgc
      121 cggtcattcc gccgatagaa agtccgcaaa aatttacacg ctggaggttt agcgcgtcca
      181 gcaactgaag cacatcacgc cctagcatct ctaccgaata ttcgccttgg gtcgcggacg
      241 tctggccatg accacgcgag tcatagcgta gtacacgaaa gcgctgagag aaaatcggca
      301 attgagagtc ccatagcgac agatttgtgc ctagtgaatt ggaaaggacc aacgtgggcg
      361 catcggcgga gccgtccagt tggtaatgca tacgcagatc tttggtttca acaaagggca
      421 caacggcacc gcctaggaat ttttatgcga tttatagttt ttaatggctg cgtcaataaa
      481 ctcgcttgct atgccggtat aatttcgcgg gtcaaaaagt ttatccagtt ggtctgatga
      541 aaggtgcttg gcgattttct ggtcgtcact tataacgtct ctaagatgac gcttttcttt
      601 agtggctcgt tcgctggccg cttcgattag ctcacgcgct tgcgagcggt cgattttctc
      661 gcccagcgca gcggtaattg cttccgcgaa aatcaggcct ctggttaact caagattttc
      721 gcgcattcgt tgcacatcga tctctagccg agggataagg gtggttagct gacgcagcgc
      781 tgcggccgtg aggcacacaa tttccggcag ggtttcccat tcggcatgcc aaccgcccag
      841 accgcgctcg tcttcctgaa tcatggcgct caacatgatg cttactaatc ctggcatgcg
      901 tatagcggcg gcgagaatca tggcgcaggc gacggggttg cgcttgtggg gcatggtgga
      961 tgatccgccg cgaccttctt cagcgggctc gcgtagttcg tcaatttcgg tttgcatgtg
     1021 gagggaaatg tctctcgcga ttttgcctaa cgtgccagtc aataatccca gggtagtggc
     1081 aatttcagcc aggcgatcgc gatgagtgtg ccagggcaat tgcggcaaag gaagtttaag
     1141 gtgctcgctc aaatttctag cgacagcccc gcctcgcgaa cccaacgctg ccagtgttcc
     1201 tgccgcgccg ccgaattgca gcaccaggca acgattttgc acttcgcgaa aacgttcgcg
     1261 atgtcgtccg agaacgtcca accatccggc gaatttt
//
LOCUS       DATCBB010000216         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_169096, whole genome shotgun sequence.
ACCESSION   DATCBB010000216 DATCBB010000000
VERSION     DATCBB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169096"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VII37_02450"
     CDS             complement(<1..587)
                     /locus_tag="VII37_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068610.1"
                     /translation="MAKKNLRTAIFLAVVFTAIVVVRARTVGARDTANGPQVSVSSYD
                     MVVANGRVMDPETGLDAVRNVGITAGKIRAISVEPLNGKTVLDAKGLVVAPGFIDLHE
                     HAQEPRNYQFQARDGVTTSLELEVGTDDVDGWYAKREGKSLINFGVSVGHIPVRMKVM
                     HDPGNFLPTGDAAHREATPRELEEIKTQIHRGLQRG"
     gene            586..1689
                     /locus_tag="VII37_02455"
     CDS             586..1689
                     /locus_tag="VII37_02455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5068611.1"
                     /translation="MNTPVSIHEILHVRRARIKDIHMPSDVEWLASYNGSELGWKGLL
                     MNYRRFGRTGWNVSEIGYGMWGMAGWTGSEDQESLASLQHAVDLGCNFFDTAWAYGDG
                     HSETLLGKIIRANPLKTLYAATKIPPRNRQWPSQRGFSLDESYPPDYIEEYVHKSLGN
                     IGVSKLDLIQFHTWEDHWLDDDRLLKAIEKLRTGNEVRAVGISINRWEPWNGIRAVNS
                     GLIDAVQVIYNIFDQNPEDELFPGCREKNVAVIARVPFDEGTLTGTLTKESKWPKGDW
                     RNTYFVADNLKSSVEHADALKPLLRDGMTMPEMALRFILNNPDVSTIIPGMRKRQNVE
                     TNLGASARGPLPGELHTALRKHRWDRTPTSWSQ"
     gene            complement(1720..2010)
                     /locus_tag="VII37_02460"
     CDS             complement(1720..2010)
                     /locus_tag="VII37_02460"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY5068612.1"
                     /translation="MRVPVKAEIFGQTYTISGELDEDYVLKLARFVDEKMRAVANATA
                     TVDTQKVAVLAALAIADELHSAQRDLGYREDVLREQAERCLVLVERALKQSA"
     gene            complement(2126..>2668)
                     /locus_tag="VII37_02465"
     CDS             complement(2126..>2668)
                     /locus_tag="VII37_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068613.1"
                     /translation="GDLDNLGALSGGLRWEETVAEHLNPARRGALFLNAEPLGVAGQL
                     SRRVAERLKFRQDVFLAEINLGQFYCAFYGVKNSRRYQTTPRFPAVERDFSLLLSDGT
                     HFSDVVKTISSLKVPEIASIEAADLFRGKNVPAGKYSLLVRIMLQPHSATLTDGQIGD
                     ISARIVSALEKELGAQLRTA"
BASE COUNT          619 a          704 c          763 g          582 t
ORIGIN      
        1 ccgcgttgca atccgcgatg aatttgagtt tttatctctt ctaactcgcg cggcgtcgct
       61 tcgcgatggg cggcatctcc ggtcgggaga aaatttcccg gatcgtgcat cactttcatg
      121 cgcaccggaa tgtggcccac gcttacacca aagttgatga gagatttgcc ttcacgcttg
      181 gcgtaccacc cgtctacgtc gtcggtgccg acttcgagct cgagagacgt ggttacacca
      241 tcgcgagcct gaaactggta gttacgcggt tcctgggcat gttcatgaag atcgataaag
      301 cctggcgcga cgaccagacc tttagcgtcg agaacagtct ttccgtttaa cggttcaacg
      361 gagatcgccc gaattttccc cgccgtgatg cccacatttc gcaccgcatc gaggccagtc
      421 tcggggtcca tcacgcggcc attggcgacg accatgtcgt agctggatac ggaaacttga
      481 ggcccattag cggtatcgcg ggctcctaca gttcgtgcgc gaacgacaac gatggcagta
      541 aataccacgg ccaagaagat cgccgtacgc agattttttt tggccatgaa tactcccgtc
      601 tcaattcatg agattctgca cgtgcgcaga gcgagaatca aagatattca catgccttcc
      661 gatgtggaat ggcttgctag ctataatgga agcgagctcg gttggaaagg acttttaatg
      721 aactaccggc gatttggacg cacgggttgg aatgtcagcg agattggtta cggaatgtgg
      781 ggcatggccg ggtggaccgg atcggaggat caagagtcgc tcgcgtcact gcaacacgcg
      841 gtggatttgg gatgcaattt ttttgatacc gcctgggcct atggggatgg ccacagcgag
      901 accctactcg ggaaaatcat tcgcgccaat ccgctgaaga cgctgtacgc agcgaccaag
      961 ataccgccca ggaatcgcca atggccaagc cagcgcggat tttctttgga cgaatcctat
     1021 ccgccggact acatcgaaga gtatgttcac aaaagtctcg gaaatattgg cgtcagcaag
     1081 ctggatctaa ttcagtttca cacctgggaa gatcactggc tggacgacga tcgattgttg
     1141 aaagccattg aaaagctgcg caccggcaac gaagtgcgcg cggtgggaat cagcatcaat
     1201 cgctgggagc cgtggaatgg tattcgcgcc gtgaattcgg ggctgatcga tgcggtgcag
     1261 gttatctaca atatctttga ccagaacccg gaagatgaat tattcccagg gtgtcgcgag
     1321 aaaaacgtgg cggtgattgc gcgcgtgccg ttcgacgagg gcacgcttac gggaacgctc
     1381 accaaggaaa gcaaatggcc caagggcgat tggcgcaaca cctatttcgt ggcagacaat
     1441 ctgaagagca gtgtcgagca tgccgacgcg ctgaagccct tgctgcgtga cggcatgacc
     1501 atgccggaaa tggcgctgcg atttattctg aataatccgg acgtgagcac cattattccc
     1561 ggaatgcgta aacgccaaaa tgtcgaaacg aatctgggcg cgagtgcccg cggaccgttg
     1621 ccgggagaac tgcacacagc gttgcggaaa catcggtggg atcgtacgcc aacttcatgg
     1681 tcgcagtaaa tgcgtagacc taaagtgcgt cgtgatccgc taggcggatt gtttcagggc
     1741 gcgctcgacg agtaccaggc agcgctcggc ttgttcgcgc aatacgtctt cccggtagcc
     1801 taaatcccgc tgcgcgctgt gcaattcatc agcgatggcc agcgccgcta gtacggccac
     1861 tttttgcgtg tccactgtag ccgtggcatt cgctacggct cgcatttttt cgtccacgaa
     1921 gcgcgccaac ttcaagacgt aatcctcgtc cagctcccca ctaatcgtat atgtctggcc
     1981 gaaaatttct gcttttacgg gcacgcgcat gaaactcgtt tctgtgaagt aactgcggcc
     2041 aagatagcac agtcattggc actctgctgc atgtggttgt tgaaaaagtg attgtcgcag
     2101 aaaggatgtt caccacaatc gcgggtcacg cggtgcgcaa ctgcgcgcct agttcttttt
     2161 ccagcgcgga gacgatgcga gcggagatgt cgccgatttg cccgtccgtc agggtcgccg
     2221 aatggggctg cagcataatt ctaaccagca gggaatattt ccctgcaggc acgtttttgc
     2281 cacggaataa atcggctgcc tcgatcgaag cgatttctgg aaccttcagt gacgaaatgg
     2341 ttttgaccac atcggagaaa tgcgtgccgt cagaaagcag cagcgagaaa tcgcgctcga
     2401 ccgcgggaaa ccgcggggtc gtttggtatc ggcgcgaatt tttgacccca taaaacgcgc
     2461 aataaaattg accaagattt atttccgcga ggaaaacatc ctggcggaat ttgaggcgtt
     2521 cagcaacccg gcgggaaagc tggccggcaa ctccgagcgg ttccgcattc agaaaaagcg
     2581 cgccacgacg agcgggattc agatgttcgg cgacggtttc ctcccaacgt aagccgccgg
     2641 aaagtgcgcc aagattgtcg aggtcgcc
//
LOCUS       DATCBB010000217         1140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_233472, whole genome shotgun sequence.
ACCESSION   DATCBB010000217 DATCBB010000000
VERSION     DATCBB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233472"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..293)
                     /locus_tag="VII37_02470"
     CDS             complement(45..293)
                     /locus_tag="VII37_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068614.1"
                     /translation="MTKWTKTRRDPGRTYCDPEEAAKLRRDVQRVQELVKLGLDAEPQ
                     YVELINKLDPKMAREKRKELIKQFRDAVYDQQPDLPGR"
     gene            complement(412..>1140)
                     /locus_tag="VII37_02475"
     CDS             complement(412..>1140)
                     /locus_tag="VII37_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5068615.1"
                     /translation="RRALNTMGAVSVVPGAIGAWRVEAVRQAGGYHVDTVAEDADLTM
                     ALLRNGYRVEYEDMALAFTEAPTTANALMRQRFRWSFGILQAVFKHRGVFARKGALGF
                     VALPNILIFQILLPLVSPFIDVMFVTGVLWYFVQNYFHPESNDPASFQRLLIFFGAFL
                     VIDFIASSIAFALERRQPEAREDAWLLSQVWLQRFAYRQVFSLVLFRTLKRAFQGLPF
                     AWDKLDRTAAVNYVPAERRDSVKV"
BASE COUNT          254 a          284 c          353 g          249 t
ORIGIN      
        1 caaaaaaatg agcactgatg aggtgcgaaa agtacgcaag caaattaacg acctggtaga
       61 tcgggctgtt gatcgtaaac cgcatcgcga aactgcttaa tcaattcctt ccgtttttcc
      121 cttgccattt tcggatctaa tttgttgatt aactcaacat attgcggttc ggcatcaagc
      181 cccagtttta ccaactcttg cacgcgctga acgtcacggc gaagttttgc cgcttcttcc
      241 gggtcacagt acgtgcggcc cgggtcgcgg cgcgttttcg tccactttgt catttcagcg
      301 gagcgcttgc ccgtgggaat tcggctttgg tgtgactttt cttatcttag cctgaatgga
      361 cttgctcttt ggagcctttg tgcggcgagg gccagcccta cacttgcaac ctcagacttt
      421 tactgaatct ctgcgttcgg cggggacgta gttgacggcg gcggtgcgat ccagtttgtc
      481 ccaagcgaag ggcagaccct ggaaggcacg cttcagggtt cggaaaagga ccagggaaaa
      541 tacctggcga taggcgaagc gctgcaacca gacctggctt aggagccatg catcttcacg
      601 cgcttcgggc tggcggcgtt ccaaggcgaa ggcgatggat gaggctatga agtcgattac
      661 caggaaggct ccgaagaaaa tcaggaggcg ctggaaactg gcgggatcgt tcgattcagg
      721 atggaaataa ttttgcacga agtaccagag gacgccggtg acaaacatca cgtcgatgaa
      781 gggagaaacc agcgggagaa ggatctggaa gataagaatg ttggggaggg cgacgaatcc
      841 taaagcgcct ttgcgcgcaa atacgccgcg atgtttgaag acggcttgga gaattccgaa
      901 ggaccagcgg aatcgttgcc gcatcagcgc gttggcggtg gtgggagcct cggtgaaggc
      961 gagggccatg tcttcgtatt cgacgcgata gccgttacga aggagagcca tggtgagatc
     1021 ggcgtcttcg gcgacggtgt cgacgtgata gccgccggct tggcgtactg cttcgacgcg
     1081 ccaggcgcca atcgctccgg gaacgacgct gaccgcgccc atggtgttaa gcgcgcgccg
//
LOCUS       DATCBB010000218         1204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_201623, whole genome shotgun sequence.
ACCESSION   DATCBB010000218 DATCBB010000000
VERSION     DATCBB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201623"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1204
                     /locus_tag="VII37_02480"
     CDS             <1..>1204
                     /locus_tag="VII37_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5068616.1"
                     /translation="NGSSHLERDLGLGSLERVELMARLENAFGVRIADQAATQANTPD
                     DLAAAVMEAPESVVETGGVSAERACVKVQQLKRKAEEAGVVSAETLIDVLHYRAAHDA
                     ARSHLVITEDADGQDKTITMTFGELHAAAQRCSAELMRRGVSAGSRVALMLPTSRAFF
                     VSYAGILLAGAIPVPIYPPFRADRIEEYAARQSAILNNAGVMLLLTFRRAEAVAKLLK
                     PRVASLVAVADAEKLIEAADKAPLPSPGARPLHLSGIRGRTGKDIALLQYTSGSTGDP
                     KGVVLTHANLLANIRAVGEAVKMGPDDVGVSWLPLYHDMGLIGAWLTLLYYGIPLAVM
                     SPIAFLMRPERWLWAIHKHRGTISTAPNFAYELCVRKIADKDIEGLDLSSWRMAMNGA
                     EPVNPGTL"
BASE COUNT          234 a          264 c          440 g          266 t
ORIGIN      
        1 tgaatggttc gtcgcatctg gaacgcgatc tgggattggg gagcctggaa cgcgtggagt
       61 tgatggcgcg gcttgagaat gcgtttggtg tgcgcatcgc ggatcaggcg gctacgcagg
      121 cgaatacgcc agatgatcta gctgcggcgg tgatggaagc tccggaaagc gttgtggaga
      181 cgggtggagt ttccgcagaa cgagcatgcg tcaaggtgca acagctaaaa cgcaaggctg
      241 aagaagccgg ggtggtgtcg gcggagacac tgattgatgt tttgcattac cgcgcggcgc
      301 atgatgctgc gcgatcgcat ctggtgatta cggaggatgc ggacgggcaa gataaaacga
      361 taacgatgac gttcggtgaa ttgcatgcgg cggcgcagcg ctgctcggcg gaattgatgc
      421 gccggggagt gagcgcggga agccgcgtgg cgctgatgct gccgacgtcg cgggcgtttt
      481 ttgtttcgta tgcggggatt ttgctggcgg gggcgattcc ggtgccgatt tatccgccat
      541 ttcgggcgga tcgcattgaa gagtacgctg cacggcaatc ggcgattttg aataatgcgg
      601 gcgtgatgtt gctgctgacg tttcgtcgcg ccgaagcggt ggcgaaatta ttgaagcctc
      661 gtgttgcgtc gcttgttgcg gtggcggatg ccgaaaaatt gattgaagcg gctgacaagg
      721 cgcctcttcc ctctcctggg gcgcggccac tgcatttgag tggaatacgc gggcgaacgg
      781 gaaaagacat tgcgttgctg caatacactt ccggctcgac gggcgatccc aaaggtgtag
      841 tgctcacgca tgcaaatttg ctcgcgaata ttcgagcagt gggcgaagcg gtgaagatgg
      901 ggccggacga tgtgggagtg agctggctgc cgctgtatca cgacatgggg ctgatcggag
      961 cgtggttgac gttgctgtat tacgggattc cgttggcggt catgtcgccg attgcgtttt
     1021 tgatgcggcc ggagcgctgg ctgtgggcga ttcacaagca tcgcggaacg attagtactg
     1081 ctccaaattt tgcgtatgag ctgtgcgtgc gaaaaattgc ggacaaagac attgaggggc
     1141 tggacctgag ttcctggcgc atggcgatga atggggcgga gccggtgaat ccggggacgc
     1201 tgga
//
LOCUS       DATCBB010000219         1301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_7025, whole genome shotgun sequence.
ACCESSION   DATCBB010000219 DATCBB010000000
VERSION     DATCBB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7025"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VII37_02485"
     CDS             complement(<1..197)
                     /locus_tag="VII37_02485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5068617.1"
                     /translation="MSERRNARRYDLALPVMIRVPSDTPDIRMTGLQKGETLDISTRG
                     MYFIIEKDLPAGSELDITLTLP"
     gene            complement(352..>1301)
                     /locus_tag="VII37_02490"
     CDS             complement(352..>1301)
                     /locus_tag="VII37_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5068618.1"
                     /translation="GVGKEVVARMIHLRSKRRSQPFIKVNCAALPGELLESELFGFEQ
                     GAFTGAVRAKPGKFEMANKGTIFLDEIAEMTTHLQAKLLHVLQDHQYSRLGGRHLVET
                     DVRVLAATNVDVQEAMKTGRFREDLYYRLNVLSINVPPLRERTSEIPLLFRHFLQKYS
                     EKFGKTPQGPSKYLLDAAVNYPWPGNLRELENFVKRYVILEDDEGSLRELIEMSSARQ
                     RTSPRAEPAVHREPQGLKALVRGLKDEAEMEAIADALEKTRWCRKDAAKMLGISYKAL
                     LYKMRQFNLDTPRTRRASVPAPAAAAEEPTTIGIGGRNE"
BASE COUNT          281 a          370 c          335 g          315 t
ORIGIN      
        1 ggtagtgtga gggtgatatc caattctgag ccggctggca aatccttctc gataatgaag
       61 tacatacctc gcgtggaaat gtcgagcgtt tcgcccttct gcaagccggt cattcgtata
      121 tcaggagtat ccgagggaac gcgaatcatc accggtagag cgagatcgta tctcctggca
      181 ttacgtcgtt ccgacattcc ttgcatcctc agtaaacgcg gcggcaatat ggcctgattc
      241 gcgcctcctt acgagtgacc ttactgtaaa tcgcgcgaag ccgccacgtt gtaacagcta
      301 acagccggaa caggtgttct aaagataaac ggctcgtact gggagacgag cctactcgtt
      361 ccggccccct ataccgatgg tcgtaggttc ttcagctgct gctgccggcg cgggcacgct
      421 ggcgcgcctc gtccgcggcg tatccagatt aaactggcgc attttgtaca gcagcgcctt
      481 gtaactgatg cccaacattt tcgcggcatc cttgcggcac caccgggttt tctccagcgc
      541 atcggcgatg gcttccattt ctgcttcgtc ctttaggccg cgcaccagag ccttcaatcc
      601 ttgcggctca cgatgcacag caggctccgc gcgcggcgaa gtacgctgtc tcgcggaaga
      661 catttctatc agctcccgca agctgccctc gtcatcttct aggatgacgt aacgcttcac
      721 aaagttctcc agttcgcgca aattacctgg ccacggataa ttcaccgcag catccagtag
      781 atacttggag ggaccttgcg gtgttttgcc gaatttttca ctgtatttct gaagaaaatg
      841 acggaacaaa agtggaattt ccgaagtgcg ctcacgcagt ggcggaacat ttatcgagag
      901 cacgttcaag cggtagtaaa gatcttcgcg gaatcgcccg gttttcatcg cttcctgaac
      961 gtcgacgttc gtggctgcca gaacgcggac atccgtctcc acaagatgcc ggccgccgag
     1021 acgggagtac tgatgatctt ggagcacgtg taaaagtttg gcctgcagat gcgtggtcat
     1081 ttccgcgatt tcgtcgagga aaatcgttcc tttattggcc atctcgaatt tgccgggctt
     1141 ggcgcggact gcccctgtaa acgcgccttg ctcgaaaccg aataattcgg attccaataa
     1201 ctctccaggc aaggccgcgc aattcacttt tatgaaaggc tggctgcgcc gctttgaacg
     1261 caaatgaatc atgcgcgcca ctacttcttt accaactccg c
//
LOCUS       DATCBB010000220         1776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_104765, whole genome shotgun sequence.
ACCESSION   DATCBB010000220 DATCBB010000000
VERSION     DATCBB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104765"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VII37_02495"
     CDS             complement(<1..620)
                     /locus_tag="VII37_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5068619.1"
                     /translation="MKKSRGRTPTKSLRRRDFLIKTTGGVLATAAFGAVPLRGWAADP
                     VNIGGIYPVTGSFAQIGQACANAAKLAVEMVNNAGGIKSLGGAKLNLILSDVQSDTTV
                     TRTETDRLISGNKLSAIHGCYASALTLIASEVAERAKMPLLTGSSSDLLNKGRHYTFT
                     PFARASQFAKAQLQMSRLVNDKPKVAVIFENTAFGTSTSNGLRELAP"
     gene            complement(1447..>1776)
                     /locus_tag="VII37_02500"
     CDS             complement(1447..>1776)
                     /locus_tag="VII37_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068620.1"
                     /translation="EARQAIECIAKDASRASDVVARVRSLAKRSPPQKQLLNINETAR
                     EVIILTASENQNNHISLHAQFADGLPLILGDRVQLQQVILNLILNAIEARSNFFIFVK
                     ITSGMAT"
BASE COUNT          366 a          485 c          503 g          422 t
ORIGIN      
        1 ggcgccagct cgcgcaggcc gttggatgtc gaggtgccga acgcggtgtt ttcgaagatg
       61 accgccacct tcggcttgtc gttcaccagc cgcgacattt gcagttgagc cttggcgaat
      121 tgcgaagcgc gcgcaaacgg cgtgaaagta tagtgacggc ctttgttgag caggtccgag
      181 ctcgagccgg tcagcagcgg cattttggcg cgttccgcca cttcgctggc gatcagcgtc
      241 aaggcgctgg catagcagcc gtggatcgcg gataatttgt tgcccgagat caaacgatcg
      301 gtttccgtgc gcgtgacggt cgtgtcgctc tgcacatcgg agaggatcaa attgagcttg
      361 gcgccgccca gcgacttgat accgccggca ttgttgacca tctcaaccgc cagtttggcg
      421 gcattggcgc aagcttggcc gatttgcgcg aagcttccgg tgaccggata gatcccgccg
      481 atatttaccg ggtctgccgc ccaacctcgg agcgggaccg cgccgaatgc agcagtcgcg
      541 agtacaccgc ccgtggtctt aatgaggaag tctctgcggc gtagcgattt tgtcggagtc
      601 cggccccggc tctttttcat attcgcctcc ctgcggcgtt atagaaatcg cgtttcctgg
      661 cacgacccgt ggcagagatt tttgccatct tattgtcctg ccgatacgac tatagccctt
      721 gttgggcagc taacaataga gccagctcgg catgtgcgag cgcctcttgt cgtcgcttag
      781 gccaaaattc ttctgcagcg atgctctaaa cgcgcatcga aggtcgacca actaatattc
      841 gccttaagtc cttcgctcgt cagcgttcgc gcaaccctga tatttcattt tgggcgcaat
      901 taggactgct acccgggcat gcagtctgga tcaaaccagt ctccgcaccg cgactccgca
      961 aaacgggaat tattcaataa ttacgtggag acttttggag caatttggga atttgggttc
     1021 gattccggaa cgcgcagaga ttcgctgtgt cgcaaaagcc cgctaacggc gggccaaatc
     1081 gtgctttaga tcgagcgcgg tccggagacc ggactggctg gctggcgcag cagtgcagat
     1141 gcccctctgc tctgcctgtt ttccctgcta acagggaatt ttacaggcaa ttttgcaaaa
     1201 ctcccggatt tgggaggcca gagaccgcaa ataatggggt cattacaggg gcttctggtg
     1261 caaattccct actcagcgga acagggaaac ttggcgcgag aacagggaat tttatcggcc
     1321 ggagttgaaa ttgttgccgg ctcgagattc ccggcacaaa gggtcgctgg gttatgtccg
     1381 tcgttatcac gaacggacat aacgcacccg cctcacttct ggatcaaaat ccgctttgtg
     1441 cttttatcag gtagccatgc cggacgttat cttaacgaaa atgaagaagt tactcctagc
     1501 ttcaatggcg ttgagaatca ggttgaggat gacctgctgc aattgtacgc ggtcacccag
     1561 aataagtggc agcccatcag cgaattgcgc gtgcagcgaa atgtggttgt tctgattctc
     1621 gctggccgtc aaaatgatga cctcacgggc ggtctcgtta atgttcaaca actgtttttg
     1681 aggagggctc cttttggcaa ggctacggac ccgcgctacc acgtcactcg cacggctcgc
     1741 gtccttagcg atacattcga tggcttgcct agcctc
//
LOCUS       DATCBB010000221         1716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_39800, whole genome shotgun sequence.
ACCESSION   DATCBB010000221 DATCBB010000000
VERSION     DATCBB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39800"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1716)
                     /locus_tag="VII37_02505"
     CDS             complement(<1..>1716)
                     /locus_tag="VII37_02505"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5068621.1"
                     /translation="IVKYDTRNPDVSMEELAPKDPPAVKNERGNVDSALASAAVKLDV
                     TYETPVETHNPMEMHATIATWNKDKLTLYETSQGVVNHRNVAAEVLNLQRESVQVISR
                     FIGSGFGCKLFPWPHSWMAALASKKLGRPVKVAVPRSMMFTTVGHRPFTRQHIQLGAD
                     KDGKLTAFLHEILQPTSMVDDFVENCAEVTTMLYSCPNLRSVQHLVHKNIGTPTPMRG
                     PGRTPSLFAIESAMDELAVKLNIDPLEIRLRNYAETDEGKKQPFSSKHLRECYQAGAE
                     KFGWSQRNAKVGSMTKGKEILGMGMATCTWPATRHNAEVRVTLLADGTVRASCATQDI
                     GTGTYTVFAQIISDRTGVPLDKIRVQLGDTALPPGPTSGGSAATSTVIPAIAKATDQA
                     VQSLLKVAAGTAGSPFEKLDPKSLKMSGGRVHKDADSAESGTPFPEILKLRKLSGLDG
                     QVRTEAPPEAKEFSMHSFGAHFCEVAFDPEIARLRITRWLSVIDGGRMINRKTSRNQI
                     LGAVVMGIGMALFEETIYDPRNGKPVNNNFADYLVPVNTDIPELDCMFLDFPDTKINE
                     YGARG"
BASE COUNT          327 a          433 c          558 g          398 t
ORIGIN      
        1 tgccccgcgc accgtattcg ttaatttttg tgtccgggaa atcgaggaac atgcagtcga
       61 gttcagggat gtcggtgttc acgggcacga ggtagtccgc gaaattatta ttcacgggct
      121 tgccgttgcg cgggtcgtaa atggtttctt caaagagggc catgccgatc cccatgacga
      181 ccgcgccgag aatctggttg cgcgaggttt tgcgattgat catgcggccg ccgtcgatca
      241 cgctgagcca gcgggtgatg cgcaggcgag caatctcagg gtcgaaagcc acttcgcaga
      301 aatgcgcgcc gaaggaatgc atggagaatt cctttgcttc gggcggggct tcggttctta
      361 cctggccgtc caggccggag agtttgcgga gtttcagaat ttccgggaag ggagtaccgc
      421 tttcggcgga gtcggcgtcc ttatgcacgc ggccaccgga catttttaga gattttggat
      481 cgagcttctc gaaaggcgac cctgctgtgc ccgcagcgac ttttagcagc gactggacgg
      541 cttgatcggt agccttggcg atggccggaa ttactgtgga ggtggccgca gaaccgccgg
      601 aggtggggcc gggtggcagg gcagtatcgc caagctgcac gcgaattttg tcgagaggca
      661 cgccggtacg atcggaaata atctgtgcga aaacggtgta ggttccagtg ccgatatctt
      721 gcgtggcaca cgaagctcgg acggtgccat cggcgagcaa ggtgacgcgg acttccgcgt
      781 tgtgacgggt cgcgggccag gtgcacgtgg ccatgcccat acccagaatt tccttgccct
      841 tggtcatgga gccgactttg gcgttgcgct ggctccaacc aaatttttcg gcgccggcct
      901 ggtaacattc gcgcaaatgt ttactggaga atggttgttt tttgccttcg tcggtttccg
      961 cgtagttgcg caggcgaatt tccagcgggt cgatgttcag tttgacggcc agttcatcca
     1021 tggcggattc aatggcgaag agcgaaggcg tgcggccggg accacgcatg ggcgtgggtg
     1081 ttccgatgtt tttgtgcacc aggtgctgga cggaccgcag attcggacaa ctgtaaagca
     1141 tggtggtgac ttcggcgcaa ttttccacga agtcgtcaac catggaagtg ggctgcaaaa
     1201 tttcgtgcag gaaggcggtg agcttgccgt ctttgtccgc gcccaactga atgtgctggc
     1261 gagtgaaggg gcggtgccca acggtggtga acatcatgct gcgtggaacg gcaactttta
     1321 cggggcggcc aagttttttg gaggccagcg cggccatcca ggagtgcggc cagggaaata
     1381 atttgcaacc gaagccggag ccgatgaaac gcgaaatcac ttgcaccgat tcgcgctgca
     1441 ggttgaggac ctccgccgct acgttgcgat gattcacgac tccttgcgaa gtctcgtaca
     1501 gcgtcaattt atctttgttc caggttgcga tggtggcgtg catttccatg gggttgtggg
     1561 tctcgaccgg agtctcatag gtgacgtcga gttttacagc agcggaggct agcgcgctgt
     1621 ccacgttgcc gcgttcgttt ttgaccgcgg gcggatcctt gggagccaac tcctccatgg
     1681 aaacatcagg attgcgcgtg tcatatttca cgatca
//
LOCUS       DATCBB010000222         1075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_136962, whole genome shotgun sequence.
ACCESSION   DATCBB010000222 DATCBB010000000
VERSION     DATCBB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136962"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..>1075
                     /locus_tag="VII37_02510"
     CDS             80..>1075
                     /locus_tag="VII37_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEY5068622.1"
                     /translation="MILGNVAVRVLEWRAQCFSDPVDRLQFLRRNIEPRPKSLSRTRR
                     LRYLALAIISGLAVATSGLFFTSTRGVTAAASSRYSPRDRYPTHSPIVDGAAEPAGPV
                     WLVKNTKQFDLYSNGLRIENQYMTTTDARRYLAFPRTGPRLGTAVWRSEPSGIVFHTT
                     ESHIAPFEEDQNQKLQRAGEGLLEYVNRSSAYHFVIDRFGRVFRVVRESDYANHAGNS
                     IWADNSWIYINLNQSFFGVAFEAQSSSGELPVTAAQVHAGRVLTEMLRARYGISAANC
                     VTHAQVSVNPGNLRAGYHLDWAANLPFSQLGLADNYRRALPSVTLFGFEADPALVA"
BASE COUNT          238 a          307 c          313 g          217 t
ORIGIN      
        1 agaggtgtgg tagcagacgg aagctcgctg gttgaacgcg caatactccc atggaggcgc
       61 tagctagcga tctatcatca tgatcctcgg aaacgtggcg gtgcgggtac tggagtggcg
      121 cgcccaatgc ttctccgatc cggtggatcg cttacagttc ctgcgacgca acattgagcc
      181 gcgtccgaaa tcgctgagtc gcacaagaag gctcagatat ttggcgctgg ccatcatatc
      241 gggactcgct gtggcaacca gcggcctgtt ctttacatcc acgcgtggcg tgacggcggc
      301 cgccagttcg cggtacagtc cacgcgaccg atatcccacg cattcaccga ttgtggatgg
      361 cgccgctgaa cccgccggcc ccgtttggtt agtaaagaat acgaagcaat tcgatttgta
      421 cagcaacggc ctgcgaattg aaaaccaata catgacgacg acggacgcac ggcgttattt
      481 ggcgtttcca cgtacgggtc caaggctggg caccgcagtc tggcggtcag aaccatccgg
      541 aattgtgttc cacaccaccg agagccacat cgcgccgttc gaggaagacc aaaaccagaa
      601 attacagcgc gccggggaag gactgctcga gtacgtaaat cgcagctccg cttatcattt
      661 cgtaatcgat cgttttggcc gcgtctttcg agtagtgcgc gaaagcgact acgccaatca
      721 tgcgggtaat tcgatatggg cggacaactc ttggatttac atcaacctca atcaaagttt
      781 ctttggagtg gccttcgaag ctcaaagcag ttcgggcgaa cttccagtaa ccgcggcgca
      841 agtacacgcc ggccgcgttc tgacagaaat gctgcgagct cgctacggga tatcagcagc
      901 caactgcgtg acgcacgcgc aggtctcggt gaatccaggc aacttgagag ccggatatca
      961 cctggattgg gcagccaacc tgcccttcag ccaactgggc ctggcggaca actatcggcg
     1021 ggcgctcccg agcgtgaccc tgtttgggtt tgaggcggac cccgcgctag tggcg
//
LOCUS       DATCBB010000223         1045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_7076, whole genome shotgun sequence.
ACCESSION   DATCBB010000223 DATCBB010000000
VERSION     DATCBB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7076"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..>1045)
                     /locus_tag="VII37_02515"
     CDS             complement(119..>1045)
                     /locus_tag="VII37_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068623.1"
                     /translation="DEQLFWLDNQPKPSNENDMNWAISYVVEPDYLKTMRIPLLRGRF
                     FTEADNENSPHVAVIDEALARKFFDAENPIGRHITFQHGGDPAEIVGLVPHVKQWGLD
                     NDDAQQLHSEMYVPFMQLPDEAMKLAPTGTTIMVRSDGSNPAMFEILRTVNKQMSGEQ
                     VIYGAQTMQEIIADSLAARRFSMILLGVFAALALILSSIGIYGVISYLVGQRTHEIGV
                     RIALGARRRDVLALILSHGARLTFLGIAIGIAASLGLTKLMSRMLYGVSASDPLTFFC
                     VAAILAFVALAACYIPTRHAMRVDPLVALRCD"
BASE COUNT          227 a          284 c          328 g          206 t
ORIGIN      
        1 gtcggtgacc gtgctgcgag gcacggtcgc gacgggcact gctctagcga agccgaagca
       61 gtagcaccgc cgttcgcgtg tattctacgc gccttaaact cattccaatc tcttgccgct
      121 aatcgcagcg taaggcgacc aggggatcga cgcgcatggc gtggcgcgtg gggatatagc
      181 aggcggccag cgcgacgaac gccaaaattg ctgcgacgca aaaaaacgtg aggggatcgg
      241 aagcgctcac gccgtagagc atgcgggaca tcagtttggt gaggcccagg gaagcggcga
      301 tcccgatggc gatgccgaga aaagtcagcc gggctccgtg cgacaaaatg agcgcgagaa
      361 cgtcgcggcg tcgcgcgccc aaggcgatgc gcacgccgat ttcgtgggtg cgctggccaa
      421 ccaggtaaga aattacgccg tagattccga tactcgagag aatcagcgcg agcgccgcga
      481 atacgcccag taaaatcatg gagaagcgcc gcgctgcgag cgaatcggca ataatttcct
      541 gcatggtctg cgcgccgtag atgacctgct caccgctcat ctgtttattc acagtgcgaa
      601 ggatttcaaa catcgcggga ttggagccat cggagcggac catgatggtg gtgccggtgg
      661 gcgccagctt catggcctca tctggcaatt gcataaacgg aacgtacatt tcggagtgca
      721 gttgttgcgc gtcatcgtta tccagccccc attgtttaac gtggggcacc aggcccacga
      781 tttccgccgg gtcgccaccg tgctggaagg tgatgtgcct gccaatggga ttttcggcgt
      841 caaaaaattt tcgagccaag gcttcatcga tcaccgcaac gtgcggagaa ttttcgttat
      901 ccgcctcggt aaagaagcgg ccacgcagga gcgggatgcg catggtcttc aggtagtccg
      961 gctcgacaac gtagctgata gcccagttca tgtcgttttc gttggatggc ttgggctgat
     1021 tgtccagcca gaagagttgc tcgtc
//
LOCUS       DATCBB010000224         1054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_71558, whole genome shotgun sequence.
ACCESSION   DATCBB010000224 DATCBB010000000
VERSION     DATCBB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71558"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..754)
                     /gene="efp"
                     /locus_tag="VII37_02520"
     CDS             complement(188..754)
                     /gene="efp"
                     /locus_tag="VII37_02520"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY5068624.1"
                     /translation="MSSTKATQLRPGMVIQHEGQLYTVFSVDHRTPGNKRGSMQTRLK
                     NLRTGTMIDYRFRAEEFVDRAILDEVEFEYLYSEGDDFHFMNTENYEQMQMTRDELGE
                     TVFYLIPNTKVKVEFFEEKAIGVDLPDTMDMKVVSTEPTLQKATASAVMKPATLETGL
                     VINVPPFVNEGDRVKVDTTEARYVQRVE"
BASE COUNT          231 a          318 c          272 g          233 t
ORIGIN      
        1 ccagctccgc cacgtcgacg ccatcttcgc ccgcgtcttc ggagcgaagc aaaacaaaac
       61 caaaaccgcg gtctgaacaa aaaagccggc tgaaaaatcc agccggcgtt gacagcctct
      121 tctagatgaa aaacgatcta gctgcggagg cggaaaacca aactcgcaac actacactaa
      181 ccgaaaatta ctccacgcgc tgcacgtaac gcgcttcggt ggtgtccacc tttacacggt
      241 cgccttcatt tacaaacggg ggcacgttga tcaccagtcc ggtctccagc gtcgcgggct
      301 tcatcaccgc gctggccgtg gctttctgca gcgtcggttc ggtggacacc actttcatgt
      361 ccatggtgtc cggcaaatcc accccgatgg ctttctcttc aaaaaattcc actttcacct
      421 tggtgttggg aatcaggtag aacaccgttt cgcccagctc gtcgcgggtc atctgcatct
      481 gctcgtaatt ttcggtgttc atgaagtgaa aatcgtcgcc ttcgctgtac aggtattcaa
      541 attccacttc gtccagaatg gcgcggtcca caaattcttc cgcgcggaag cggtaatcga
      601 tcatcgtgcc ggtgcgcagg tttttcaggc gggtctgcat ggaaccgcgc ttgttccccg
      661 gcgtgcggtg atccacgctg aaaacggtgt acagctgccc ttcgtgctgg ataaccatgc
      721 cggggcgaag ttgtgtggct ttcgtgctgc tcataatctc ccagttaact tgtatttcgg
      781 aatttcgccc ggcgctgtgt gccaacccga aaagcggcaa actcaggccc gcgaaatcac
      841 tcgagggacg ccggtgaatc gctgccggcg cggacattca gtataaatgg ccgcattcca
      901 tgcggtcaat ctgcgcacgg aagtttcccc gccatacatc ctcgacaaag tgttcgcggc
      961 tccatatgct gaactgcgat gagcactgct gcacaaccgg ctgagccaaa ggtccgcgtc
     1021 cgctccaggg acaaggcgaa agacttttgg cacc
//
LOCUS       DATCBB010000225         1031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_137053, whole genome shotgun sequence.
ACCESSION   DATCBB010000225 DATCBB010000000
VERSION     DATCBB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137053"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1031)
                     /gene="pcaF"
                     /locus_tag="VII37_02525"
     CDS             complement(<1..>1031)
                     /gene="pcaF"
                     /locus_tag="VII37_02525"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:TIGR02430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY5068625.1"
                     /translation="DWQAVDEVILGCVNQAGEDNRNVARMALLLAGLPKEVPGYTVNR
                     LCASSMEGVASAARAIKTGEADLVIAGGVESMSRAPFVVGKSDSAFSRAMKMEDTTMG
                     WRFVNPLMKVKYGTDTMPETGEIVAEEYRISRQDQDAFALRSQQRAAAAMANSILAQE
                     IVSVPLPQKKGPPIHFSADEQPRPDTSLEGLAKLKGVVKPDGTVTAGNASGINDGSCA
                     ILMASEAGAKRHGLTPRARVVTFAAAGVAPRIMGIGPVPATRKALTRAKLSLDDMNVI
                     ELNEAFASQALAVLRELHLPDDAAHVNPNGGAIALGHPLGASGARLITTATYQLSRTR
                     EKYALCTMC"
BASE COUNT          185 a          297 c          294 g          255 t
ORIGIN      
        1 atgcacatgg tgcatagggc gtatttttcg cgtgtacgcg atagttgata cgtcgctgtg
       61 gtgatcagtc gtgcgccgct ggcgcccagc ggatgcccca gcgcgatggc tccgccgttg
      121 ggatttacgt gtgcagcgtc gtccggcagg tgcaattcgc gcaagaccgc gagagcttgc
      181 gacgcgaacg cttcattcaa ttcgatcaca ttcatgtcat ccaaagataa ttttgctcgg
      241 gtcagcgcct tgcgagtcgc tggcactggg ccgatgccca tgattctggg cgctactccc
      301 gccgctgcga aagtgactac tcgagcccgc ggagttagac catgacgttt ggcaccggct
      361 tccgatgcca ttagaatcgc gcaagagccg tcgttaattc ctgatgcgtt gccggctgtg
      421 actgtgccgt ctggttttac cactcctttt aattttgcga gcccttctag tgaggtgtct
      481 ggacggggct gttcatccgc cgaaaagtgt atcggtgggc cttttttttg tggcaggggc
      541 acagacacaa tttcctgggc aaggattgag ttggccatag ccgccgcggc gcgttgctgg
      601 ctccgcagcg cgaatgcatc ctgatcctga cgcgaaattc tatattcttc ggctacaatt
      661 tcgccggttt ccggcatcgt gtctgttcca tatttcacct tcatcagcgg atttacaaaa
      721 cgccagccca tggtagtgtc ttccattttc atcgcgcggg aaaaagcgga gtcggacttg
      781 cctaccacaa atggtgcgcg tgacatgctt tccacgccgc cggcaattac caggtcggct
      841 tcgccggtct tgatggcacg tgcggcgctg gcaacccctt ccatgctgga agcgcagagg
      901 cgatttactg tgtagcccgg cacttccttg ggcaagcccg cgagcagaag agccatgcgg
      961 gccacgttgc gattatcttc ccccgcctgg ttcacgcatc cgaggatgac ctcgtctacg
     1021 gcttgccaat c
//
LOCUS       DATCBB010000226         1044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_71589, whole genome shotgun sequence.
ACCESSION   DATCBB010000226 DATCBB010000000
VERSION     DATCBB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71589"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VII37_02530"
     CDS             <1..376
                     /locus_tag="VII37_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068626.1"
                     /translation="LNLVEKRWNSKDNAKAVIEQSKDLRKKIGAVEDELIQVNSKASE
                     DELNYPTKMNSWLGYLQAAVDSADTAPSEGELGVFAELNQKLEAQLAKWHELLSTDVP
                     AINAAMQKNGIALISAAPEASK"
     gene            complement(413..>1044)
                     /gene="surE"
                     /locus_tag="VII37_02535"
     CDS             complement(413..>1044)
                     /gene="surE"
                     /locus_tag="VII37_02535"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY5068627.1"
                     /translation="TLRQPIVCHAIAEREWAIDGTPADCVIVALHRLLPERPDMVISG
                     INFGANLGENVYYSGTVGAAREAALHHIPAVAMSLCSKVPKVSFENAARVARLAAEIV
                     LKEGLPDQVLLNVNVPEPWTGGVCFTRQSKKITRNQLSEGKDPRGRSYFWLFEQKIDK
                     DVEPETDYAAIFSGSVSITPLHLDPTHTESLNHLSHWAEPIAAAFKRKS"
BASE COUNT          279 a          299 c          258 g          208 t
ORIGIN      
        1 actcaacctg gtcgaaaagc gctggaattc caaagataac gccaaagccg tcatcgaaca
       61 gtccaaagat ctacgcaaaa aaattggcgc cgtcgaagac gaattgattc aggtgaactc
      121 caaggccagc gaggacgaac tcaattaccc aacgaagatg aatagctggc tcggctattt
      181 gcaagcagcg gtggatagcg ccgacaccgc accgtcagaa ggagaattgg gcgtcttcgc
      241 agaactaaat caaaaactgg aggcgcaact cgccaaatgg cacgagctgc tgtcgaccga
      301 tgttccagcc atcaacgcgg caatgcaaaa gaacggcata gcactgattt ctgccgcgcc
      361 ggaagccagt aaatgaaggc tagttcttaa ttagatgggc gaacgcgctg attcaacttt
      421 tgcgcttaaa tgctgcagcg atgggctcag cccaatgcga caaatgattt aacgactcgg
      481 tatgcgtggg atccaggtgt aacggcgtaa tcgacacgct gcctgaaaaa atagcggcgt
      541 aatccgtctc cggttccaca tccttatcaa ttttctgctc aaatagccaa aaataggaac
      601 ggccgcgcgg gtctttgccc tcgctcagct gattcctcgt aatcttcttc gattgccgcg
      661 tgaagcaaac gccaccagtc cagggctccg gcacattcac attcaacagc acctgatccg
      721 gcaggccttc tttcaaaaca atctccgccg ccaacctggc aacccgcgcc gcattttcaa
      781 aactgacttt gggaactttg gagcacagcg acatagccac cgccggaata tgatgcagtg
      841 cggcttctcg cgcagctcct acggtccctg agtaataaac gttctcgccc agattcgcgc
      901 cgaaatttat tccggaaata accatgtccg gccgctccgg caaaagtcta tgcagcgcca
      961 cgatgacgca atcggcgggc gtgccgtcga tagcccattc gcgttccgca atggcatggc
     1021 aaacaatggg ctgccgcagc gtca
//
LOCUS       DATCBB010000227         1314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_233625, whole genome shotgun sequence.
ACCESSION   DATCBB010000227 DATCBB010000000
VERSION     DATCBB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233625"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VII37_02540"
     CDS             <1..820
                     /locus_tag="VII37_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5068628.1"
                     /translation="ALELSQDRLAEKALFTRLKIPVAAHCAIDSREELILATQKIGLP
                     GVLKTRRLGYDGKGQYALHAPADVDRAWAAIGGASLIYEKFQDFSREVSLVGVRSASG
                     QAIFYPLSCNTHAGGILRYNTAPFTNARLERSARLYLKRVMSALDYIGVLTIEFFVVR
                     GSLIANEMAPRVHNSGHWTIEGCVTSQFENHLRAVCDMPLGSTRALGHTAMINFLGLM
                     PNRERLLAIDGLAYHDYGKTARPGRKLGHCTILKRLPKERDTALPNTLKLIEWA"
     gene            869..>1314
                     /locus_tag="VII37_02545"
     CDS             869..>1314
                     /locus_tag="VII37_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5068629.1"
                     /translation="MYLELFKLHELPFRLSPDPQFLYLSKQHARAKAYMESTIWFTDG
                     FVVITGEIGAGKTTLIETFLRELQSDAIVAQINQTQLSPTAFLQTVLVQFGFSPFNMK
                     KPEVLATLNQFLVEQHSNGRKVLLIIDEAQNLTQRVLEEVRMLSGV"
BASE COUNT          300 a          360 c          369 g          285 t
ORIGIN      
        1 ggcgctcgag ctatcgcaag atcgcctcgc ggaaaaagcg cttttcaccc gcctcaaaat
       61 ccccgtagcc gcacattgcg cgatagacag ccgcgaggaa ctgatccttg caactcagaa
      121 aatcgggctt cccggcgtgc taaaaacgcg gcgcttgggt tatgacggaa aagggcaata
      181 cgcgctgcac gcgccggcgg atgtcgatcg ggcttgggct gccattggcg gtgcgagcct
      241 gatctacgag aaatttcaag atttttcccg cgaggtgtcg ctggttggcg tgcgttcagc
      301 gagtggacag gcgatatttt atcccctgtc ctgcaacacg catgcaggcg ggatcttaag
      361 gtacaacact gcacctttta caaatgcgcg tctggagcgc agcgcgcggc tgtacttgaa
      421 acgagtcatg agcgccctcg actacatcgg tgtgctgacc atcgagtttt tcgtggtgcg
      481 aggatctttg atcgccaatg agatggcgcc tcgcgtacat aactcgggcc attggaccat
      541 cgaggggtgc gttaccagtc agttcgaaaa tcacctgcga gccgtctgcg atatgccgct
      601 cggcagcact cgtgccctcg ggcacacggc catgatcaat tttctagggc tgatgccgaa
      661 tcgcgaacgc ttgctggcga ttgacggatt ggcgtatcac gactacggca aaacagcgcg
      721 cccgggccgc aagttgggcc attgcaccat tcttaagcgc ctgccaaagg agcgcgatac
      781 cgcgctgccg aatacactaa aattgatcga atgggcctaa actaagtagt ttccggcaga
      841 ccagcaaccc cttgaattga cgagcgatat gtatctagaa ctcttcaaat tgcatgaatt
      901 gccgttccgc ttgagcccgg atccgcaatt tttgtatttg agcaaacagc atgcgcgcgc
      961 caaggcatac atggagtcga ccatctggtt caccgacggc ttcgtcgtga tcaccggtga
     1021 aatcggcgcc ggcaagacca ccttgatcga gacgtttttg cgtgaactgc aaagcgacgc
     1081 gatcgtggcg caaatcaacc aaacgcaatt atctcccacc gcgtttctac agaccgtcct
     1141 cgtgcagttc ggcttctcgc cgttcaatat gaaaaaacct gaagtgctgg cgacgttgaa
     1201 tcaatttttg gtggaacagc acagcaacgg acgcaaggtg ctgctgatca ttgatgaggc
     1261 gcagaatttg acccagcgcg tgctcgagga agtgcgcatg ttgtcgggcg tgga
//
LOCUS       DATCBB010000228         1618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_461644, whole genome shotgun sequence.
ACCESSION   DATCBB010000228 DATCBB010000000
VERSION     DATCBB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461644"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VII37_02550"
     CDS             complement(<1..1253)
                     /locus_tag="VII37_02550"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068630.1"
                     /translation="MAAPGFGKDMFSNLKAELPGQLPAYLKGQRWFGAKARELRATEV
                     VDFIALERDALHALIVMVKVEYEGGGADLYSVPLVVSKDPGSKDEPGAPGKGLAALTV
                     SGRNNGETLTLSDALKNETFLSLLLDAIERGLVFTGEAGELVTSHTHALSMQETGAAG
                     SLRPRAITAEQSNSSVAYGDRLILKFFRRLEEGENPDLEMGGFLTEKAHYQHTPQLLG
                     ALEYRGARGVRMTQGILQGFVPNQGDAWQYTMKSISNFYNEVGKGSAESSVAELQNNA
                     ARASIATFLESVGLLARRTAELHLALASPAAVTEADFAPEPFDDKFQRSFEDALLELT
                     NRIFGQLRHAQDRLPGNARPKVEKVLASEPEIIERFHAALSKPIRAVRTRIHGDYHLG
                     QVLYTGADFVIIDFEGEPARPLSQRR"
     gene            complement(1278..>1618)
                     /locus_tag="VII37_02555"
     CDS             complement(1278..>1618)
                     /locus_tag="VII37_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068631.1"
                     /translation="FIAMLILFLGFVLANFLSRAALLASVNTGIKSPKIWSWSIRFVI
                     WILAISMALEELGLARQTVLAAFSIVFGASMLGLAIAFGLGGQDLARRALERYLGNSG
                     KEKYKEPQPL"
BASE COUNT          385 a          554 c          343 g          336 t
ORIGIN      
        1 cgcctctgcg acaacggccg cgccggctcg ccctcaaaat caataatcac gaagtccgca
       61 ccggtgtaca gcacctgccc aaggtggtag tcgccatgta tcctcgtccg cactgcgcgt
      121 attggtttgc tcagcgcagc atggaaacgt tcaataatct ccggctcact cgccagcact
      181 ttctctactt tcggtcttgc attccccggc agccggtcct gggcgtgccg cagctgcccg
      241 aaaatccgat tcgtcaactc cagcagcgca tcttcaaaac ttcgctgaaa cttgtcatca
      301 aaaggctcag gagcaaaatc agcttccgta acagccgccg gcgacgccag tgccagatgt
      361 aattccgctg tccgccgcgc aagaagccct actgactcca aaaatgtagc gatcgatgcc
      421 cgcgcagcat tattctgcaa ctccgcaacc gagctctctg ctgaaccctt gcccacctca
      481 ttgtaaaaat tggaaatcga cttcatggtg tactgccagg catctccctg gttcggcacg
      541 aagccctgaa gaatcccctg cgtcatgcgc actccgcgtg ctccgcgata ttcgagcgct
      601 ccaagcaact gcggtgtgtg ttggtagtgg gccttctccg taagaaaacc acccatctcc
      661 agatccgggt tctcgccctc ttccagccgc cgaaaaaatt tcaatatcag tcggtccccg
      721 taagcaaccg agctattact ctgctccgct gtaatcgcgc gaggacgcaa gctcccagcc
      781 gcccccgttt cctgcatcga cagcgcgtga gtatgcgacg tcaccagctc gccggcttcc
      841 ccagtgaaaa ccagtcctcg ttcaatcgcg tccagcaaaa gagaaaggaa agtttcgttt
      901 tttagcgcgt cgctgagcgt taatgtctct ccattgttcc gcccagaaac cgtcagcgcc
      961 gccagacctt ttccgggtgc cccgggttcg tcttttgaac cggggtcttt actcacaacc
     1021 agcggcacag aatacaaatc cgcaccaccg ccctcatatt ccaccttcac cataacgatc
     1081 agcgcatgca gcgcgtcacg ttccaacgca ataaaatcca caacttccgt tgcacgaagc
     1141 tcccgagcct tagccccgaa ccaacgctgc cccttcaagt aagcgggcag ctgccccggt
     1201 aattccgcct tcaaattaga aaacatatct ttgccaaaac ccggcgcggc cataaacatt
     1261 cttctccatc gagtgcatca caacggttgc ggttctttat atttctcctt ccccgaattt
     1321 cccagatatc gctccaacgc gcgccgcgcc agatcctgcc ctccaagtcc aaacgcaatt
     1381 gccagcccca gcatcgatgc tccaaaaaca atcgaaaacg ctgccagcac cgtctgccgc
     1441 gccagcccca gttcctccag cgccatggag atcgccaaaa tccagatcac aaatcgaatc
     1501 gaccagctcc agattttcgg cgacttaatt cccgtgttca ccgatgccag cagcgccgcc
     1561 cgcgacaaaa aatttgccag cacaaatccc agaaacaaaa tcaacatggc aataaaca
//
LOCUS       DATCBB010000229         1058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_233667, whole genome shotgun sequence.
ACCESSION   DATCBB010000229 DATCBB010000000
VERSION     DATCBB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233667"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VII37_02560"
     CDS             complement(<1..607)
                     /locus_tag="VII37_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068632.1"
                     /translation="MIRRGLLALVIFLSFQLRTAAQSSFTLEQVMSAPFPANLTASKT
                     GGRVAWTLDQQGRRNIWVAEAPGFHVRQLTHSDTDEGQPLSDLHFSSDSNVLVYVRGE
                     GKNAAGQSPNPTSNTAGFEQSVWSIAWTGGEPKKLDAGDSPQISPAGTVAYIKDHQLW
                     MAKLDASENPYQIVVRGNSADPQWSPDGTKLAFVSSRTDHAF"
     gene            complement(698..>1058)
                     /locus_tag="VII37_02565"
     CDS             complement(698..>1058)
                     /locus_tag="VII37_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068633.1"
                     /translation="GVGKVRLDNLEFRGWDVNASVADGEAHPGVSNWPSGVGTFTVRD
                     RNVFLEDVRLDGGTQFTLIDGTVSFGREADLAVETSAGRRAVRNANGPGQVLKIVGPL
                     DGPRITREKNASRQPAD"
BASE COUNT          229 a          293 c          293 g          243 t
ORIGIN      
        1 tgaaggcgtg atcggtgcgc gatgaaacga aggcaagttt ggtgccgtct ggcgaccact
       61 gcggatccgc gctgttgccg cgcacgacaa tttgatacgg attttcggat gcgtcgagtt
      121 tagccatcca taattggtgg tctttaatat aggcgacggt tcccgcaggg gaaatctgcg
      181 gagaatcacc ggcgtccagt ttttttggct cgccaccggt ccatgcgatc gaccaaacgg
      241 attgttcgaa gcctgcggtg ttgctggtgg gattaggaga ctgcccggcg gcgttttttc
      301 cttcgcctcg cacatatacg agcacattgc tgtccgacga aaaatgaagg tccgaaaggg
      361 gctgaccttc gtcagtatcg gaatgcgtca gttgccgcac atgaaaaccg ggggcttcgg
      421 caacccagat atttctgcgg ccctgttgat cgagtgtcca agccacgcgc ccgccagttt
      481 tggaagcggt taagttcgca ggaaatggtg cgctcattac ctgctcgagc gtgaaggagg
      541 actgcgcggc ggtacgcagc tgaaacgata aaaagattac gagcgcaagc aatcctctac
      601 gaatcatgaa caggctcctc gccgacctaa tttccgctgc agcgttttct ggcacgatac
      661 tttgattctt caagagcttt cagtggtgga ctgaaactta gtccgccggt tgacgtgagg
      721 catttttttc tcgcgtgatg cgtggcccat cgagcggccc aacaattttc agcacctgcc
      781 cgggcccgtt ggcgttgcgc actgcgcgcc ttcccgcgct ggtttcaaca gcaagatcgg
      841 cttcgcgtcc gaaactaacc gtgccgtcaa ttagagtgaa ctgcgtaccc ccatccagtc
      901 gtacatcttc caagaaaaca ttgcggtcgc gaacggtgaa cgttccaacg ccggagggcc
      961 aattggaaac tcccggatga gcttcaccat ctgcaacgct ggcattcaca tcccagcccc
     1021 gaaattcaag attgtccaag cgaactttgc cgactccg
//
LOCUS       DATCBB010000230         1139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_363792, whole genome shotgun sequence.
ACCESSION   DATCBB010000230 DATCBB010000000
VERSION     DATCBB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363792"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1139)
                     /locus_tag="VII37_02570"
     CDS             complement(<1..>1139)
                     /locus_tag="VII37_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5068634.1"
                     /translation="GGTFGGPIKKDKLFFFGAYEGQRYSVGNPTLLSYPSLDPKAMPF
                     AGNTPVTTSVIQACLNVPAASRSVTSLKMAGLDATCTRTSGYSIFDLGSSFARLRDSN
                     GALGGNVSGNLNTDYAVNGYMGKADYNLNDKNTINAKYFLGNHTGLVVNSATITQPYW
                     RPTDTANVQFFGAQWNYIASSSMFNTVRVGYNRFYQKFETSDCPGAGTAPDYGIPFGY
                     GTTKPNCGFTNITLSPFTGAIGCCSSFPKYYGPDNILEFHDGFSYLRGKHNFKVGGEF
                     RNSEAGDGGVYNRGRGQVTFSTLETFFSGTPSGNGQIFIGDPLRDMKQKAMAAYFQDD
                     WRILPRLMLNLGIRYEYETPLTEGNNMMSNFIPGTGFTQLGVNTN"
BASE COUNT          231 a          304 c          348 g          256 t
ORIGIN      
        1 tggttggtat tgaccccgag ctgcgtaaag ccggtaccgg gaatgaagtt cgacatcatg
       61 ttattcccct ccgtcagcgg cgtttcgtac tcgtagcgta ttcccaggtt cagcatcagg
      121 cgcggcagga tgcgccagtc atcctggaaa taagcggcca tcgccttttg cttcatgtcg
      181 cgcagtggat cgccgatgaa aatctgcccg tttccgctgg gcgttccgga gaaaaatgtc
      241 tccagggtag agaacgtgac ctgtccgcgg ccgcggttgt aaaccccgcc gtctccggct
      301 tcgctgttgc ggaactcgcc gcccaccttg aaattgtgtt ttccgcgcag atacgagaag
      361 ccgtcgtgaa actccagaat attgtccggt ccataatatt tggggaaact ggaacagcag
      421 ccgatggcac cggtgaaggg gctgagcgta atgttggtga acccgcaatt gggtttcgta
      481 gtgccataac cgaaggggat gccgtagtcc ggtgcggttc ccgcgccggg gcaatcgctg
      541 gtttcaaact tttggtagaa ccggttgtag ccaacccgca ccgtgttgaa catggaagaa
      601 ctcgcaatgt agttccattg cgcgccaaaa aattgcacgt tagcggtatc ggtgggacgc
      661 cagtagggct gtgtgatggt cgcgctgttc accacgagtc cggtgtgatt gcccaggaaa
      721 tacttggcgt tgatggtgtt tttgtcgttc aaattgtagt ccgcttttcc catgtagccg
      781 ttcacggcgt agtccgtatt gaggttgccg gagacgttcc cgcccagagc gccgttggaa
      841 tctcgtaggc gcgcgaagga agaaccgagg tcaaagatcg agtagccgga cgtccgcgtg
      901 caagtcgcat cgagccccgc catcttgagg ctggtaacgc tgcgagacgc cgcggggacg
      961 ttcaggcacg cctggatgac gctggtggta actggagtgt tcccggcgaa aggcatggcc
     1021 ttcggatcca ggctgggata cgacagcagc gtcggattgc ccaccgaata gcgctggcct
     1081 tcataggctc caaaaaagaa gagcttgtcc tttttgatcg gcccgccgaa agtgccccc
//
LOCUS       DATCBB010000231         1089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_71852, whole genome shotgun sequence.
ACCESSION   DATCBB010000231 DATCBB010000000
VERSION     DATCBB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71852"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VII37_02575"
     CDS             <1..249
                     /locus_tag="VII37_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068635.1"
                     /translation="ALGVAYAETAFSRLVGREPQIPVEGVKIAQHLMFVDCTRAQREL
                     GFRAQPISAAFERAVRWYEKNGYVNNRRIKRMAHAVPA"
     gene            265..1026
                     /locus_tag="VII37_02580"
     CDS             265..1026
                     /locus_tag="VII37_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068636.1"
                     /translation="MNILITYAVEAEFAPWRALNMEKVAIGDITLQRAQIGRAAVDFV
                     ITGMGSENATRVADAVISNAYSFCIISGFAGALKSSVKVGEVVVSERVQHQASAQSEI
                     CDRDLLIHASENGATRIATLLTTDHVVNTAVEKNNLSAFADAVDMESFAILHVANQKN
                     VPSAVLRVISDSFDRDMPAELDTIVDPQGHVKIFGVARYVAKHPLMVPALVRLGRDSK
                     TASEALAHFLESYINKLSSATHRRPAQAMQEVATS"
BASE COUNT          261 a          326 c          272 g          230 t
ORIGIN      
        1 gccttaggtg tagcctatgc ggagaccgcg ttttcgcgcc tggtcggtcg cgaaccacaa
       61 atcccggtcg aaggcgtcaa aatcgcccaa catctaatgt ttgtggattg cactcgcgcg
      121 caacgggaat tgggctttcg cgcccaacct atatcggcag cattcgaacg ggccgtccgc
      181 tggtacgaaa aaaatggcta cgtgaacaat cggcgcatca aacgcatggc ccacgccgtg
      241 cccgcgtaac taggcgatat tacgatgaac attctcatca catacgcggt cgaagccgaa
      301 ttcgccccct ggcgcgcgct gaacatggaa aaagtggcca ttggtgacat cactttgcaa
      361 cgcgcccaaa tcggcagagc agcagtcgac tttgtcatca ccggtatggg atcggaaaac
      421 gcaacgcgag tcgcggacgc cgtcatttcc aacgcatatt ctttttgcat catctcagga
      481 ttcgcaggcg cgttgaagtc ctctgtcaaa gtgggagagg ttgtggtgtc cgaaagagtt
      541 caacatcagg ccagtgccca atccgaaatc tgcgaccggg atctgttaat ccatgcatcc
      601 gaaaatggcg caacacgcat tgcaacgttg cttaccaccg accatgttgt aaataccgcg
      661 gtggaaaaaa ataacctatc cgctttcgcc gatgcggtag acatggaaag cttcgcaatc
      721 cttcatgtcg cgaaccaaaa aaacgtaccc agcgccgtac tgcgtgtaat ttctgattcc
      781 tttgatcgcg atatgcccgc tgagctcgac accatcgtgg atcctcaggg ccatgtgaaa
      841 atttttggcg tcgcccgtta cgtcgccaag catcctttga tggttccagc attggtgcgt
      901 ctcggacgcg acagtaaaac cgcctccgaa gcgctggcgc actttctcga gtcctacatc
      961 aacaaattgt cttccgctac gcaccgccgt ccggcccaag ccatgcagga agtagccacc
     1021 tcatgagcga caccggcaca ctgctttacg cgcccgcaac tctcggcccc gtgcccaaaa
     1081 ccatgcacg
//
LOCUS       DATCBB010000232         1925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_233861, whole genome shotgun sequence.
ACCESSION   DATCBB010000232 DATCBB010000000
VERSION     DATCBB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233861"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VII37_02585"
     CDS             <1..832
                     /locus_tag="VII37_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5068637.1"
                     /translation="PQNLALLGEVRWRRLRGLDVAMIFQEPMTSLNPVMRVGEQIAEA
                     IAAHFPNTNKQKLNYWVLEALRLAAVPEPSVRAGQFPHQLSGGLRQRVMIAMAIAAGV
                     SDSEDEPTPLEFRKPGTPLTKPRLLIADEPTTALDVTVQKQILELLDGLRRRLGLGLL
                     FITHDLGVVARVADRVAVMYAGRIVEQGRVGDVLRTPRHPYTQGLIAASPTLERGKLS
                     PIPGIVPQLTELPPGCSFEPRCPVRLPECRLAVPELRDAGHNQAARCVLVGKEVVLKQ
                     "
     gene            877..1701
                     /locus_tag="VII37_02590"
     CDS             877..1701
                     /locus_tag="VII37_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5068638.1"
                     /translation="MTTGEISISSFLSVRELTKRYSRSGGPFKRASEHEQFVAVDHVG
                     FEVTQGETFAVVGESGCGKTTLARMLLRLIEPDSGQIVFAGRDLLALPAAELRAQRRE
                     MQMIFQDPFASLDPRMRVGEIVGEPLAIHEPKLSKAERASRTLAILERVGLRADALRR
                     YPHEFSGGQRQRIGIARALILRPKLIVADEPVSALDVSVGAQVLLLLRELQSEFNLTY
                     VFISHSLPVVAQLATRIAVMRGGKFVEVGEAEQVLHQPEHDYTRELLAAVPALPVG"
BASE COUNT          400 a          547 c          566 g          412 t
ORIGIN      
        1 accgcaaaac ctggccctgc taggtgaagt ccgctggcgg cggctgcgcg gccttgacgt
       61 tgcgatgatc tttcaggaac ccatgacttc gctgaatccg gtaatgcgtg tcggggagca
      121 gatcgctgaa gcgattgccg cacactttcc gaacacaaat aaacagaaac tcaattattg
      181 ggtgctcgaa gcgctgcgcc ttgcggctgt gcccgagccc tcggttcgcg ccggccaatt
      241 tccacaccag ctttcaggcg ggttgcggca aagagtaatg attgcgatgg ccatcgccgc
      301 aggcgtgagc gattccgaag acgagccgac gccgctggaa tttcgcaagc ctggcacacc
      361 gctgaccaag ccgcgtttat tgattgcgga cgagcccacc accgccctgg atgtaaccgt
      421 acaaaagcaa attctcgaat tgctggacgg gctgcgacgt agacttggcc tgggcttgtt
      481 atttatcact cacgatttag gagtagtggc gcgcgtggcc gaccgcgtgg cggtgatgta
      541 tgccggacgc atcgtggaac aaggccgagt cggagatgtg cttcgtacgc cgcgccaccc
      601 ctacacccaa ggactgatcg ccgcatcgcc gacactcgag cgcgggaagc tttctcctat
      661 tcctgggatc gtgccgcagc taaccgagct cccgccggga tgttccttcg aaccgcgctg
      721 tccggtgcgg ttgccggaat gccgcttggc cgtgccggag ctgcgcgacg ccggacataa
      781 ccaggcggca cgttgcgttc tagtcggaaa agaagttgta ctgaaacaat gaaattgcac
      841 ttaagaaacg aagccgcgct caagcaatag cgttaaatga cgaccggaga aatttccatt
      901 tctagctttc tcagtgtgcg cgagctgacc aaacgttaca gccgcagtgg tggcccgttc
      961 aaacgcgctt ccgagcatga acagtttgtt gccgtggatc acgttggctt cgaggtcacc
     1021 cagggcgaaa cattcgctgt ggtgggcgag agcggttgtg gcaagaccac gctggcccgc
     1081 atgcttttgc gcttgatcga accggacagt ggtcagatcg tgtttgcagg acgcgatctg
     1141 cttgcgctgc cggccgcgga actccgcgcg caacgccgcg agatgcagat gatctttcaa
     1201 gatccctttg catccctcga tccgcgcatg cgggtcggcg aaatcgttgg tgaaccgctg
     1261 gccatccacg agccgaaact gtccaaagcc gagcgcgctt cacgcacgtt agcaattctg
     1321 gagcgtgtag gcctgcgcgc ggacgcactg cgccgctatc cgcacgagtt ttccggcggg
     1381 cagcgccaaa gaataggaat tgctcgcgcc ttaatcctgc ggcccaagtt gattgtcgcg
     1441 gacgagccgg tatccgcgct ggacgtttcc gtgggtgcgc aggtgctatt gctattaaga
     1501 gaattgcagt cggaatttaa ccttacctat gttttcatat cgcacagcct gccggtcgtg
     1561 gcgcagcttg caacacgcat tgccgtgatg cgcggcggga aattcgtcga ggtaggagaa
     1621 gccgaacaag tgttacatca gccggagcac gactatactc gcgagctttt ggctgcggtt
     1681 ccagcccttc cggtaggata aattttcgtt agctggtaat ttaattaggt ttttcgagta
     1741 atctctacgc cggccaggtt taaacacttc cattgcgagc gcgtccaccc ttagcgctgt
     1801 aaaccgaaat gatttgggcg catccgcacg aggatattca tgtccactaa tccattagca
     1861 gttcccgaag ggcagcccac cagccagcct gaaacgggcg cgttcggtcg aatgactggc
     1921 gtggt
//
LOCUS       DATCBB010000233         1195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_7497, whole genome shotgun sequence.
ACCESSION   DATCBB010000233 DATCBB010000000
VERSION     DATCBB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7497"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1195
                     /locus_tag="VII37_02595"
     CDS             <1..>1195
                     /locus_tag="VII37_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY5068639.1"
                     /translation="ARKAIREKSHIVLTNPDMLHTGILPHHTRWTRLFENLRYIVLDE
                     LPTYRGVFGSHLCNVLRRLRRIASFYGCHPQFICCSATIANPGELAGKLLETDVEVLN
                     ANGAPAAEKTFVFYNPPVVNRALGIRRSYINEACRVSQEFLKRDLQTMVFANSRLHTE
                     LMLTYLQQANPQQPGRPANIRGYRGGYLPNERRNIERGLRDGLIRGVVSTSALELGID
                     VGSLDTVVMAGYPGTIAATWQRAGRAGRRSGSSCAVLVASSAPLDQFIVRNPDYFFGN
                     SPEHAFIQPDNLEILVNHLKCAAFELPIGPEERFGAVDVQDLCARLAEAGFLHRAGEN
                     YHWTQEAYPADTVSLRSVTSDNFVIIDITGAPAVIGEVDFSSALTTVHEKAIYIHGGQ
                     QYHVEH"
BASE COUNT          253 a          334 c          337 g          271 t
ORIGIN      
        1 cgcgcgcaaa gcaatacgcg agaaaagcca tatcgtactt accaatccgg acatgttgca
       61 tactgggatt cttcctcatc acacacggtg gacgcggctg tttgagaatt tgcggtacat
      121 cgtgctggac gaactgccca cctaccgagg cgtatttggc agtcatcttt gtaatgtgct
      181 gcgacgtttg cgacgcatcg ccagttttta tggctgccat ccgcaattta tttgctgctc
      241 ggcgacgatc gccaacccgg gcgagcttgc cggcaagctg ctcgagacgg atgtcgaggt
      301 cttgaatgct aacggggcgc ccgccgcaga aaaaactttt gtgttttata acccaccagt
      361 agtgaatcgg gctctaggga ttcggcgaag ctacatcaac gaagcttgcc gcgtttccca
      421 ggaatttcta aaacgcgatc tgcaaaccat ggtttttgct aattcgcgat tacacaccga
      481 actgatgctc acttatttgc agcaggccaa tccgcagcag ccaggcagac ctgccaacat
      541 tcgcggatat cgcggcggct atctcccgaa cgagcgcagg aatatcgaac gcgggttacg
      601 cgacgggctg attcgcggag tcgtttccac cagcgccctc gagttaggca tcgacgttgg
      661 atcgctggat accgtggtca tggctggcta cccagggacc attgcagcca cgtggcagcg
      721 cgcaggccga gcgggtcgcc gcagcggcag ttcgtgtgcc gtattagtgg cctcctctgc
      781 gccattggac caattcatcg tgcgaaatcc cgattatttt tttggtaatt ctccggagca
      841 cgcatttatc cagccggaca atctggaaat tctcgtcaat catttgaaat gcgcggcttt
      901 cgaactgccc atcggccccg aggagcgttt tggagctgtg gacgttcagg atctttgcgc
      961 gcgactggcg gaggcgggct ttctgcaccg cgcgggagaa aattaccact ggacgcagga
     1021 agcgtacccc gcggacacgg tcagcttgcg ctcggtgacc agcgacaatt tcgtgattat
     1081 cgatattacc ggcgcgcccg cggtgattgg cgaggttgat ttttccagcg cgctgaccac
     1141 cgtgcacgag aaagccatct acattcacgg cgggcagcag tatcacgtgg agcat
//
LOCUS       DATCBB010000234         4650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_137416, whole genome shotgun sequence.
ACCESSION   DATCBB010000234 DATCBB010000000
VERSION     DATCBB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137416"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VII37_02600"
     CDS             complement(<1..802)
                     /locus_tag="VII37_02600"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5068640.1"
                     /translation="MSTLFQGGHLLSILIFFPAVGSLALLLLRSDDHLFIRRIALIVS
                     VAEFLFSLLLLRGASMASGEYRLVEYGQWITSPPIHYHLGVDGISLFLVILTTFLTPI
                     SVLASWSSIGHRVKEFFVMLLMLEVGVIGVFLSLDLFLFFLFWEVMLIPMAMLIGIWG
                     HERRIYAAVKFVLYTMAGSILMLVGIIWLYNATRTFDLPTIQQMIQTDMLVLEPRAEM
                     LLFLAFFVAFAVKVPLFPLHTWLPDAHVEAPTAGSVMLAGVLLKMGTYG"
     gene            complement(802..2787)
                     /gene="nuoL"
                     /locus_tag="VII37_02605"
     CDS             complement(802..2787)
                     /gene="nuoL"
                     /locus_tag="VII37_02605"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5068641.1"
                     /translation="MPILEHLWIIPLLPLLGATVNGLFGRTWPNKIVNSVAIGATGLS
                     FLAALEAVREFFLLSPDQIPWVKQYFSWIEAGNFRAGFDLQVDQLTVVMLLVVTGVGW
                     LIHIYSTGYMAHEGGYYRFFAYLNLFMFFMLILVLAANYVLLFVGWEGVGLASYLLIG
                     FYFLRKSAADAGKKAFIVNRIGDFGFMLGMFLLFRTFGTLDFVKLFDKATPLSAEAMG
                     HLGTLTVACLLLFMGAAGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYVVAR
                     SHVLFMHAPTAMLVVAIVGCATALFAATIGLVQTDIKKVLAYSTVSQLGYMFLACGVG
                     AFSAGIFHLMTHAFFKALLFLAAGSVIHAMGGEQDMLRMGGLSKKIKWTYVTMLTGTL
                     AIAGIPPLAGFFSKDSILLNAYQSENGGPNVGHILYAAGLFTALLTSFYMFRLIFLTF
                     FGKERYDEHHVHVHESPKSMLVPLAILAVLSIVGGWLAAPAFWGKPDYFAKFLEPVFA
                     SPGGSEAGTAEAVAHALETPLAIVAVITALAGLLAAYWLYVKQPSKPDILAKSFGGAY
                     RTLLHKYYVDEFYNAVVVRPLMWLSTNVFWKAVDVSIIDGAVNGIAEGVTVVGDGTRR
                     TQSGNARSYAVWVVVGAIVIVAVIFWPHVRPVLGVAR"
     gene            complement(2788..3090)
                     /gene="nuoK"
                     /locus_tag="VII37_02610"
     CDS             complement(2788..3090)
                     /gene="nuoK"
                     /locus_tag="VII37_02610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5068642.1"
                     /translation="MIPTNYYIVLSAILFGLGVLAFIFKRNIISIFMSIELMLNAVNL
                     AFVAFSQAMHKLDGQVFVLFVIVVAAAEAAVGLAIIILTARNRQSLNIERVDLLKL"
     gene            complement(3093..3596)
                     /locus_tag="VII37_02615"
     CDS             complement(3093..3596)
                     /locus_tag="VII37_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5068643.1"
                     /translation="MTAPLIIFFVLAALAVGGALSLIFQKHPIHSALSLIVVMVSLAG
                     LYLLMGAEFVAAVQIIVYGGAIMVLFVFVIMLLNAGIEEHTNFSKLAGFPGLLLAFAL
                     TGFIAATIARSTDTVQAASQPGNMASTRDLSTMIFTDFVYPFELTSFLILVAVLGAIV
                     LAQREKA"
     gene            complement(3593..>4650)
                     /locus_tag="VII37_02620"
     CDS             complement(3593..>4650)
                     /locus_tag="VII37_02620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY5068644.1"
                     /translation="LLQPMADGLKFLFKEDPTPAGVDRFVYFLAPLLALALALTSIAI
                     IPFGPDPLPWKILGQTIYLGILPPNVNIGILALFAITALGVYGVALAGWSSNSKYPLL
                     GGLRSSAQMISYELALTLSVVGVLLIAGSFNLTEIINDQTSRYGILGWNIFPQFLGFI
                     CFFIAAVAETNRVPFDLPEAESELVAGFHTEYASFKFAMFFIAEYTSMITVSCLCAIL
                     FFGGWLSPFPATWTWTYYLPTVLLLPAGLWVIWDGLHYETIFGKVILPGIGAVMCIMA
                     IALVVFPGVNNFIQAPFWLLSKIFVFLFVYVWMRGTLPRLRYDQLMAFGWKMLLPVSI
                     ANVVITSFAILRWGAIK"
BASE COUNT         1313 a         1463 c         1080 g          794 t
ORIGIN      
        1 ttccgtaggt gcccatcttc agcagcacgc ccgccagcat caccgatcca gcggtgggcg
       61 cctccacgtg cgcgtccggc aaccaggtgt gcagcgggaa cagcggcacc ttcaccgcaa
      121 aagccacgaa aaacgccaga aacaaaagca tttctgcgcg cggttccaac accagcatgt
      181 cggtctgaat catttgctga atagtcggaa gatcaaatgt tctggtggcg ttatacagcc
      241 agataattcc caccagcatc agaatcgatc cagccatggt atacagcacg aatttcaccg
      301 cggcataaat ccgccgctcg tgcccccaga tgccgatcaa catggccatg ggaatcagca
      361 tcacttccca gaacaagaag aacaaaaata aatccagcga caaaaacaca ccgatgacgc
      421 ccacttccag catcaacagc atcacaaaaa attcttttac ccgatgaccg atgctgctcc
      481 acgaagccag caccgagatc ggcgtcagaa acgtggtcag gatcaccagg aataggctga
      541 tgccatccac gcccaggtga taatgaatcg gcggactggt gatccactgc ccgtactcca
      601 ccagccggta ctcgccggac gccatcgacg cgcctcgcaa caacagcagc gaaaacaaaa
      661 actccgctac cgacacgatc agcgcaatgc ggcgaataaa aagatgatca tcgctgcgca
      721 acagcagcaa cgccagcgat cccacagcag ggaagaagat caatatcgaa agcaaatgcc
      781 cgccctggaa aagtgtgctc atcagcgcgc cactcccaga accggccgca catgcggcca
      841 gaaaattacc gccacaatca caatggctcc aaccactacc cacaccgcat agctccgcgc
      901 atttccagat tgtgttcgcc gcgtgccatc accaaccacc gtgacgcctt cagcgattcc
      961 attcaccgcg ccatcaatga tcgacacatc cacggccttc caaaaaacat tcgttgaaag
     1021 ccacatcagc ggtcgcacta ctactgcgtt atagaattca tcaacataat atttgtgcag
     1081 cagcgtgcga tacgccccgc caaacgattt cgccagaatg tccggcttgc ttggctgctt
     1141 cacatatagc cagtacgccg caagcagccc tgccagcgcg gtaatgaccg ccacgatagc
     1201 cagcggtgtt tccaatgcgt gcgccaccgc ttccgctgtc ccagcctcgc tcccgccggg
     1261 cgatgcgaag accggctcca aaaacttcgc aaagtaatcg ggcttgcccc agaacgccgg
     1321 cgcggccagc catccgccca cgatggaaag cactgcaaga atcgccagcg gcaccagcat
     1381 gcttttcggt gactcgtgaa catgcacgtg gtgctcgtcg tagcgctctt tgccaaaaaa
     1441 cgtcaggaaa atcagccgga acatgtagaa cgatgtcagc agcgcggtaa acaatcccgc
     1501 ggcataaagt atgtgcccga cgttaggccc gccattttca ctttgataag cgttcagcaa
     1561 aattgagtct ttgctgaaaa atccagcgag cggcggaatt cccgcaatcg ccagcgtgcc
     1621 cgtgagcata gtcacgtagg tccacttaat tttcttgctc aatccgccca tgcgcagcat
     1681 gtcttgctcg ccgcccatcg catggatcac gctgcctgcc gccaaaaaca acagcgcctt
     1741 gaaaaacgcg tgcgtcatca aatgaaaaat tcccgccgaa aatgcgccga cgccacaagc
     1801 cagaaacatg tagccaagct ggctgaccgt cgaataagcc aacactttct tgatgtccgt
     1861 ctgcactaat ccaatcgtcg ccgcaaataa tgccgtagcg cagcccacta tcgcgaccac
     1921 aagcatggcc gtgggtgcgt gcataaacaa cacatgcgac cgcgccacca cgtaaactcc
     1981 tgccgtaacc atggtggctg cgtgtattaa cgcgctgacg ggcgtcgggc cttccatcgc
     2041 atccggcagc cacacataaa gaggaatctg cgccgatttg cccgccgcgc ccatgaacag
     2101 cagcaagcac gccactgtca gcgtgcccag atgccccatg gcctccgcgg acaatggcgt
     2161 ggccttatca aacaacttca caaaatccag cgtgccaaac gtgcggaaca gcaaaaacat
     2221 ccccagcatg aagccaaagt cgccgatgcg attcacgatg aacgcttttt tgcctgcatc
     2281 cgccgcagat tttcgcaaaa aataaaaccc gatcaacaaa tagctcgcca gccccacgcc
     2341 ttcccagcct acaaacagca aaacgtaatt cgcggccagc accaggatca gcatgaaaaa
     2401 catgaacaaa ttgaggtacg cgaagaagcg atagtagccg ccttcatggg ccatatagcc
     2461 ggtggaataa atatggatca gccatcccac gccggtaacc accagcagca tcactaccgt
     2521 cagttgatcc acttgcagat cgaagcccgc gcgaaaattt ccagcctcaa tccacgaaaa
     2581 atattgcttc acccagggaa tttggtccgg cgaaaggagg aaaaactcac gcacggcctc
     2641 gagcgctgcc agaaaagaca gcccggtggc gccaatcgcc acgctgttga cgattttgtt
     2701 cggccaagtc ctgccaaaca atccgttcac cgtcgcgcca aggagcggca acagtggaat
     2761 gatccacaga tgttcaagta tgggcattta gagtttcagc aggtccacac gctcgatgtt
     2821 cagcgattgc cggtttcgcg ccgtcaggat aataatcgcc aagcccactg cagcttccgc
     2881 cgcagccacc acaatcacga acaaaacaaa tacctgcccg tccagcttat gcatcgcctg
     2941 gctgaacgcc acaaacgcca ggttcacggc attcagcatc aactcgatgg acataaaaat
     3001 cgaaataatg ttgcgcttga aaataaacgc cagcacgccc agcccgaaca aaatggcgct
     3061 cagcacgatg taataattcg tggggatcat cgtcaggcct tctcccgctg cgccagcacg
     3121 atcgcgccca gcacggccac cagaataaga aatgaagtca gctcgaaggg atagacaaaa
     3181 tccgtgaaaa tcatggtgga taaatctctg gtcgacgcca tattgcccgg ctgcgatgcc
     3241 gcctgcaccg tgtccgtcga gcgcgcgatg gtcgcggcga taaatcccgt caatgcgaac
     3301 gccaatagca ggccgggaaa tcctgcaagc ttcgaaaaat tggtgtgctc ctcgatcccc
     3361 gcattcagca acatgatcac aaacacaaac agcaccatga tcgcgccgcc atacacaatg
     3421 atctgcactg cggccacaaa ctccgcgccc atcaacaggt acaagcccgc cagcgacacc
     3481 atcaccacga tgagcgacaa cgcgctgtga atcgggtgct tctgaaaaat caagctcagc
     3541 gcgccgccca ccgccagcgc cgccagcaca aagaaaatga tcagcggtgc ggtcatttta
     3601 tggcgcccca ccgcagaatc gcgaagctgg tgatcaccac gttggcgatg gaaaccggca
     3661 gcagcatctt ccaaccgaag gccatcagct ggtcgtaacg caaacgcggc aaagttccgc
     3721 gcatccacac atacacaaac aagaacacaa aaatcttcga gagcaaccag aacggcgcct
     3781 gaataaaatt atttacaccc gggaaaacca ccagcgcgat ggccatgatg cacatcaccg
     3841 cgccgatccc cggcaaaatc accttgccaa aaatagtttc gtagtgcagc ccgtcccaga
     3901 taacccacaa acccgcgggc agcagcaata ccgtcggcaa ataataggtc cacgtccagg
     3961 tggcggggaa gggagacagc cagccgccaa aaaacaaaat cgcgcacaag caagacaccg
     4021 tgatcatgct ggtgtattcc gcgataaaaa acatcgcgaa tttaaaactc gcatactcag
     4081 tatgaaatcc ggccaccagc tccgattcgg cttccggcaa atcgaatggc acgcgatttg
     4141 tttccgccac cgccgcgata aagaagcaga tgaagcccag aaattgcggg aaaatgttcc
     4201 agcccagaat tccgtaccgg ctggtctgat cattaataat ttccgtcaaa ttaaacgatc
     4261 ccgcgatcag cagcacgccc accacggaaa gcgtgagcgc caattcatag ctgatcatct
     4321 gcgccgaact gcgcaagcca cccagtaacg gatacttgct gttcgaagac caccctgcca
     4381 gcgccactcc atacacgcct agagcggtga tcgcgaacaa agccaaaatc ccgatgttca
     4441 cattgggcgg caaaatcccc agataaatgg tctggcccaa tattttccag ggcagcggat
     4501 ccggcccaaa cggaataatc gcaatggaag tcaacgccag tgccaacgcc agaagcgggg
     4561 cgagaaaata aacaaagcga tccacgccag cgggagtcgg atcttcttta aataaaaatt
     4621 tcagcccatc ggccatcggc tgcagcagcc
//
LOCUS       DATCBB010000235         1282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_364055, whole genome shotgun sequence.
ACCESSION   DATCBB010000235 DATCBB010000000
VERSION     DATCBB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364055"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /gene="hflX"
                     /locus_tag="VII37_02625"
     CDS             <1..912
                     /gene="hflX"
                     /locus_tag="VII37_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY5068645.1"
                     /translation="RLGGKSGGGGAGGAGGAGRIGVRGPGEKKLETDRRRIRDRVGKI
                     QNGIADLRKQRALRREARNAVPLGTIALVGYTNAGKSTLFNALSRAEVLVSARMFATL
                     DPTIRAVRLPSNRRVLVSDTVGFIRDLPKSLLTAFRATLEEVQEAALILHVSDISNAH
                     HDELDEEVHKILEELGVVDRPILRVLNKVDRLTDKERKNLKAGANGKSNEGPVLVSAL
                     TGEGIEELLRRIDVALPTDPLVSLSLRLPLAQGRTLALIHALGRVLRSEVEDEDMLLD
                     AEVPVSIARRLRLNDYVVEGTSRLALT"
     gene            938..1138
                     /locus_tag="VII37_02630"
     CDS             938..1138
                     /locus_tag="VII37_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068646.1"
                     /translation="MAAFVLWCILFILCWPVALLALVLYPFVWLITLPFRIVGIAVHG
                     VFGLLSAMFLLPVRLLRGPRAI"
BASE COUNT          265 a          278 c          443 g          296 t
ORIGIN      
        1 cggttgggcg gcaaaagcgg aggcggcgga gcaggcggcg ccggaggagc aggacgcatc
       61 ggcgttcgtg ggcctggcga aaagaaactg gagacggacc ggcgaaggat tcgagaccgg
      121 gtgggcaaga ttcaaaacgg catagcggat ttgcgcaagc agcgcgcgtt gcggcgggaa
      181 gcgcggaatg cggtgccgct gggtacgatt gcgctggtag gttataccaa tgcgggaaag
      241 tccacgctgt ttaacgcgct gagccgggcg gaagttttgg tttctgcgcg gatgtttgcg
      301 actttggatc ccacgattcg agcggtccga ttgccgtcga accggcgggt gctggtttca
      361 gacacggtgg gattcatacg cgatctgccg aagagtttgc tgacagcgtt tcgggcgacg
      421 ctggaagaag tgcaggaagc ggcgttgatt ctgcatgtca gcgatatatc caacgcgcat
      481 cacgatgagc tggacgaaga ggtgcacaaa attctggagg aacttggggt tgtcgaccgg
      541 ccgatattgc gggtcttgaa taaggtggac cggctgacgg ataaagaaag aaaaaacctg
      601 aaggctggcg ccaacggaaa gagtaatgag ggacctgtgc tggtgtcggc gctgaccggt
      661 gaaggcatcg aggaattact gcggcgcatc gatgtggcgc tgccaacgga cccgctggtt
      721 tccctttcgt tgcgattgcc gttggcgcaa ggacggactt tggcgttgat tcacgcgctt
      781 ggaagggtgt tgcgatcgga agtggaggat gaagatatgc ttttggatgc cgaggtgccg
      841 gtctcgatcg cgcggcgttt gcggctaaat gattacgtgg ttgagggaac ttccaggctg
      901 gcgctgacgt aattataagt gggcagagga ggtaatcatg gccgcgtttg ttttgtggtg
      961 cattcttttt attctttgtt ggccggtggc gctgctggcg ctggttcttt atccctttgt
     1021 gtggctgatt acgctgccat ttcgaattgt gggaatcgct gtgcacggag tgtttgggtt
     1081 gctgtccgca atgtttttgc ttcccgtaag gttactgcga ggcccccggg cgatttgaag
     1141 tcgcataaga aaacattttg caaattaatc cacgaaagct cccagccacg aaatgcggct
     1201 tccggcagca gtgatcgcga gacgattaga gcagttaaag ccgtgatagc gatgcgattt
     1261 tgagcgtcct ggaaattctc gg
//
LOCUS       DATCBB010000236         1306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_396905, whole genome shotgun sequence.
ACCESSION   DATCBB010000236 DATCBB010000000
VERSION     DATCBB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396905"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VII37_02635"
     CDS             complement(<1..459)
                     /locus_tag="VII37_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068647.1"
                     /translation="MPWIVSALLFFAPFMDQTPEDEAIQQLSKVRRIYVAVLTGGDAA
                     LQIRELLMTCLHNSKQFIITEEEDKADAVLKGAGDDDVFTDTFQSSEGINAHSQIGAS
                     SSEGLRNYSSSSSSRSGALTIGENESRRTEERKHEAIATVRLVNKDGDVIW"
     gene            complement(463..930)
                     /locus_tag="VII37_02640"
     CDS             complement(463..930)
                     /locus_tag="VII37_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068648.1"
                     /translation="MTPKKLVALGILTTMLCMAISAHGLGGHAEYVGGTITQIPAGCR
                     GIMQAVDEQYFVFYSGKASWRVPYQKINLVEYGEKVDRRYIAAVLISPLFLLAKKRQH
                     FLTVGYSDDDDRQQAMIFKVSKDDIRAILVSLEARTGRKVEFQDEDARKSGKG"
     gene            1003..>1306
                     /locus_tag="VII37_02645"
     CDS             1003..>1306
                     /locus_tag="VII37_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5068649.1"
                     /translation="MDQNKTQEERILTAEDAGAGVIVSPDVHRANRVPPGQTRTKKWP
                     ILDANGAPPISLEHWKFELRGLVAHPMEWNWREFQEIERVKVFSDFHCVTHWSRLGN"
BASE COUNT          262 a          368 c          381 g          295 t
ORIGIN      
        1 ccagatgaca tcgccgtctt tattcaccag gcggactgtg gcgatggcct catgttttcg
       61 ttcttcggtg cggcggctct cgttttcgcc aatggtcagt gcgccggagc ggctactgct
      121 gctgctcgaa taattgcgca gaccctcgct gctgctggcg ccgatctgcg aatgcgcgtt
      181 gatgccctcc gacgattgga acgtatcggt gaacacgtcg tcgtcgcccg cacccttcag
      241 caccgcatcg gccttgtctt cttcttcggt gatgatgaac tgtttggagt tgtggaggca
      301 ggtcatcaac agttcgcgaa tctgcagtgc ggcatcaccg cccgtcagca ccgcgacata
      361 aatacgccgg actttgctca attgctggat cgcctcgtct tccggcgttt ggtccatgaa
      421 gggcgcgaaa aacagcagcg cggagacgat ccatggcatt actcacccct tcccgctctt
      481 acgagcgtct tcgtcctgga actcgacttt gcggccagtg cgcgcttcca agctcaccag
      541 tatcgcgcga atgtcgtctt tactgacctt gaagatcatg gcctgctggc ggtcatcgtc
      601 gtcggaatag cccacggtga ggaagtgctg acgtttcttg gcaaggagga agagcggcga
      661 gatgagcacc gcggcaatgt agcggcgatc gaccttctcg ccatactcca ccagattgat
      721 cttctggtag ggaacacgcc agctcgcctt cccggaatag aacacgaagt attgctcatc
      781 aacggcttgc atgatgccgc ggcagccggc gggaatttgt gtaatggtgc cgccgacgta
      841 ttccgcatga ccccccaatc cgtgggcgga gatagccatg cacagcatcg tggtgagtat
      901 gcccagcgcg accagtttct tgggtgtcat actgaagttt agttgtagga ttttccggaa
      961 aatctcaccg gaaccctccc aagctggtcg tcgatgtttg ccatggacca aaacaaaact
     1021 caagaagaac gaattctgac ggcggaggat gcgggcgccg gtgtgatcgt gagccctgac
     1081 gtccaccgcg ccaatcgagt gccgccgggc cagacgcgca cgaagaagtg gccgattctg
     1141 gacgctaatg gcgcgccgcc gatttcactc gaacactgga aatttgaact tcgtggcctg
     1201 gtggcgcatc caatggaatg gaactggcgg gaatttcagg agatcgagcg ggtgaaggtg
     1261 ttttccgatt ttcactgcgt gacgcattgg tcgcgattgg gcaaca
//
LOCUS       DATCBB010000237         1499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_105188, whole genome shotgun sequence.
ACCESSION   DATCBB010000237 DATCBB010000000
VERSION     DATCBB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105188"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..>1499)
                     /locus_tag="VII37_02650"
     CDS             complement(128..>1499)
                     /locus_tag="VII37_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068650.1"
                     /translation="ASEATREAWRIELPQWCADQRNQMTGPADEADLVVKMLNSGAPG
                     VMLDLEDSTANVWEHSQRGIANVLEALTGRLTYFDRKRDKTVAIKPSNMVIFTRARGL
                     HLHQAGVFEGEMLPASLFDVAMVAFQVDYGALKHPLCFYIPKSESAEEALWWRDLFQM
                     IAKAKGWAVNAIKCMALVESHPMAFQMEEFAWNLRDHLLGLNLGRWDYMASLIHFNLA
                     NPKWVLPDRNTIPHDVAFFQNLRKLIPEICHKHGMLAIGGMTALYPSREDAELNARAL
                     KVLAEDKKNESLSLMDGAWTGHPDQNEIAVAQFPAPNQLQARPTNAEQHPNLRPLPEG
                     VGKRTVAGTRAAIRTVIRYRNGVLNGKGASLLDGYMEDLATDRIYRLMIAQRMRHSKA
                     IEIVDETGKAVAHTREVVSQMFDEELERLVSEGAKENDPAAIARLREARRMSEEMIER
                     GEFDPA"
BASE COUNT          316 a          534 c          308 g          341 t
ORIGIN      
        1 gccctgcgcg tgatttccgt ttccattttt tccgttgctg ccgttgatcg ccatcgtctg
       61 tctccttatg tctcgtttgc tgcggggcaa aatccgagaa atcgtctaag cgcgcaccct
      121 ctgcgcatta cgcaggatca aattctccgc gctcaatcat ttcttcgctc atccgccgcg
      181 cttcgcgcaa tcgcgcaatc gccgctggat cattctcttt tgcgccttca ctcaccaacc
      241 gctccaattc ctcatcaaac atctgcgaca ccacttcccg cgtgtgtgcc accgctttcc
      301 ccgtctcatc cacaatctca atcgctttcg aatgcctcat ccgctgcgca atcatcaacc
      361 ggtaaatccg gtccgtcgcc agatcttcca tatacccatc cagcaaactc gctcccttgc
      421 cattcaaaac gccgttgcga taccgaatca ccgttcgaat cgccgcgcgc gtccccgcca
      481 ccgtccgctt gcccacgcct tcgggcaacg gtcgcaaatt cggatgctgc tccgcatttg
      541 tcggtcgagc ctgcaactgg ttcggcgccg gaaattgtgc taccgcaatc tcgttctgat
      601 ccggatgccc ggtccaagcc ccatccatca acgacagcga ctcattcttt ttgtcttccg
      661 ccagcacctt cagtgctcgc gcattcaact ccgcatcttc ccggctcgga tacagcgccg
      721 tcatcccacc aatcgccagc atcccgtgct tgtggcatat ctccggaatc aatttccgca
      781 aattttgaaa aaacgccaca tcatgcggaa tcgtattgcg atccggcaaa acccacttgg
      841 gattcgccaa attgaaatga atcaagctgg ccatgtagtc ccagcgtccc aagttcaatc
      901 ccagaaggtg gtcccgcaaa ttccacgcaa actcttccat ctgaaacgcc atcggatgcg
      961 attccaccag cgccatgcac ttgatcgcat tcaccgccca acccttggcc ttggcaatca
     1021 tctgaaataa atcgcgccac cacagcgcct cttccgccga ttccgatttc gggatataaa
     1081 aacaaagcgg atgtttcagc gcgccgtaat ccacctgaaa cgccaccatc gccacatcga
     1141 acagcgaagc cggcagcatc tccccttcaa aaactccggc ctggtgcaaa tgcagtccac
     1201 gagcgcgcgt aaaaatcacc atgttgcttg gcttgatcgc caccgtctta tcgcgctttc
     1261 gatcaaaata agtcagccgt ccagtaagcg cctcgagcac attcgcgatg ccccgctggc
     1321 tgtgctccca aacattcgcc gtcgaatcct cgagatcgag catcacgccc ggcgcgcccg
     1381 aattcagcat cttcaccacc agatcagctt catccgcagg cccagtcatc tggttgcgtt
     1441 gatccgcgca ccactgcgga agttcaatcc gccacgcctc gcgcgtcgcc tccgacgcc
//
LOCUS       DATCBB010000238         3398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_105272, whole genome shotgun sequence.
ACCESSION   DATCBB010000238 DATCBB010000000
VERSION     DATCBB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105272"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VII37_02655"
     CDS             complement(<1..819)
                     /locus_tag="VII37_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068651.1"
                     /translation="MNSAKSPNLGAVTTIGRLFAVATMVALCFAMPKPARAGRLAADT
                     IALFPKEIGEFAYADLKKARSLSWFPQLQEQLLPDRFKQFERFLASAGIDPNSQVEEL
                     AWGLVPEGVTAKTAAVGSDAVPTGEEIVGVALGNFNPSSAETYFKQQKLPTFKARGYT
                     LYAFGSGAGPTDLFFLFIDSNTAAFGHRKLLEKMIEIRFGGEEGLLRNDQFYPLINDA
                     NGTGTVWAVLNPAYTRLAMQQLAPEIQQFPEAAKLVTRMRNMIISVDASGALDGK"
     gene            complement(871..2403)
                     /gene="ftcD"
                     /locus_tag="VII37_02660"
     CDS             complement(871..2403)
                     /gene="ftcD"
                     /locus_tag="VII37_02660"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY5068652.1"
                     /translation="MTRLIECVPNFSEGRDPAKVDAIVAAMSSVPGVYVLDREMDADH
                     NRCVVTLAGDPDAVAAAALLGVGKAAELIDLTVHQGAHPRVGATDVVPFIPIEGVTLD
                     DCVALARRVGNEIWKRHRIPVFFYEAAATRPDRVNLENVRRGQFEGLREDLKRNHDRQ
                     PDVGEPKLHPTAGTTVVGARKFLIAYNVNLNTSDIGIANKIAKAIRFSSGGLRHVKSM
                     GVALKARNLAQVSINLTDFESTPMHRVYEMVKREAERYGAIPVGSEIVGLVPKKALEM
                     AADFFLQLEDFSPSQVLENRLQSALSGAPPEAPTGESKFAHLAKPFLDAVAAPTATPG
                     GGSVSAFAGALAGALGQMVAGLSRKKKSQAAHVVQLSETLDLLRRTTDDLTKAIDRDA
                     EAFDAVMEAFKLPQGEANENAARDKAIQAATRGAAEAPLQVADVTVALFEQLVQLQAI
                     AAASMRSDLQVARFMAVAGARGSLANVEINLDSLHDAAYVSKVKIKVASLRERLEKNP
                     AV"
     gene            complement(2437..>3398)
                     /gene="hutI"
                     /locus_tag="VII37_02665"
     CDS             complement(2437..>3398)
                     /gene="hutI"
                     /locus_tag="VII37_02665"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY5068653.1"
                     /translation="KRGGGILNSVNKLRAATSDTLKQRAHSALRQFAAYGTTTIEAKS
                     GYGLDLAGELKILKLHKELSAEQPLDIASTFLGAHVIPAEFRSRPSGAEKYISLLIDK
                     LLPEIAAQKLAEYCDVFCERGAFNLAQSKQILEAGKRYGLTPRIHSEQLSRTGGTQLA
                     VKLGAASCDHLEKINRADIRALAKSKTVATLLPGCNFHLGLKQYAPARDLIDAGAIVA
                     LATDYNPGTSPTVSMPMILSLACTQLRMTPAEAVSAATINPAYSLGREKQIGSLEVGK
                     LADIAIFDVDDYREIPYYFGMNTCHTTIKRGRIVYSKSPLSAN"
BASE COUNT          722 a          869 c         1022 g          785 t
ORIGIN      
        1 ctttccgtcc agcgcaccgc tggcgtccac gctgatgatc atgttgcgca tgcgcgtgac
       61 cagcttggcg gcttcaggaa attgttgaat ctctggagca agctgttgca tagctagccg
      121 cgtatacgcg ggattcagga cggcccagac cgtgccggta ccgttggcat cgttgatgag
      181 cgggtagaac tggtcattac gaagcaggcc ttcttcgccg ccgaatcgta tttcaatcat
      241 tttttcgagc agcttgcggt ggccgaaggc cgcggtgttg gaatcgataa atagaaagaa
      301 aagatcggtg gggccggcgc cgctgccgaa agcgtacaaa gtgtagccgc gggccttgaa
      361 ggtcggcaat ttttgctgct taaaataagt ttcggcgctg ctgggattga agttgccgag
      421 ggccactcca acaatttctt cgcctgtagg cacagcatca gagccgacgg cagccgtttt
      481 ggcggttacg ccttcgggaa ccaagcccca ggctaactct tcgacttgag aatttgggtc
      541 tatcccggcg gaagcgagga aacgctcgaa ctgcttgaag cggtccggca aaagttgttc
      601 ctgaagttgt gggaaccaac tgagcgaacg cgcttttttc agatcagcgt acgcaaattc
      661 gccaatttct ttggggaaca aagcaatagt gtctgccgcc agacgccccg cacgagcagg
      721 cttgggcatg gcgaaacaca gcgcgaccat cgtggctacg gcgaagagac gcccgatggt
      781 agttaccgca ccaaggtttg ggcttttagc tgaattcatc cgcggattca tttgggttcc
      841 tttcaaaatt cgacgcctgc ccgtatttct ttagacggcg ggattctttt ccaggcgctc
      901 gcgcaatgag gcgaccttaa tcttaacttt ggatacataa gcggcgtcat gcaggctatc
      961 cagatttatt tccacgttgg ccaaagagcc gcgggccccg gcaaccgcca tgaaacgggc
     1021 tacttgtaag tctgaacgca tggatgcggc ggcgatagcc tgcagttgca caagttgttc
     1081 gaaaagcgca actgtcacat cggccacctg taatggggct tcggcagcgc cgcgggtagc
     1141 agcttgaatg gctttgtctc gtgcggcgtt ttcgttggct tcgccctgtg gcagtttgaa
     1201 agcctccatt accgcatcga aagcctctgc gtcacgatcg atggccttag taagatcgtc
     1261 ggtggtgcgg cgtaagaggt cgagcgtttc cgagagttgg acgacgtgcg ccgcttgcga
     1321 ttttttcttg cgtgataggc cagccaccat ttgacccagc gcgcctgcca gcgcaccggc
     1381 gaacgcggaa acgctgcctc cacccggagt agccgtggga gcggcgacgg catccaggaa
     1441 aggctttgcc aagtgtgcaa atttgctttc gccggttggg gcttccggtg gcgcgccgga
     1501 aagagccgat tgcaagcgat tctccagaac ttgtgacggc gagaagtctt ccagttgcag
     1561 aaagaaatcc gcggccatct ccagagcttt cttgggaacc aagcccacaa tttcgctgcc
     1621 gacagggatt gcgccgtagc gttcggcctc gcgcttgacc atttcataaa cgcgatgcat
     1681 cggcgtggat tcgaaatcgg tgaggttgat cgaaacttgc gcgagattgc gcgctttcaa
     1741 cgccacgccc atggatttta cgtggcgcaa cccaccagaa gagaagcgga tggccttggc
     1801 gattttattg gctatgccga tatctgaggt gttgaggttc acgttgtagg caataagaaa
     1861 tttgcgtgcg ccgacgacgg tggttccggc agtgggatgc aattttggtt cgccaacgtc
     1921 gggttgacga tcgtgattgc gtttcaggtc ttcgcgcaag ccttcgaatt gaccgcgacg
     1981 aacgttttca agatttacac ggtctggccg cgtggccgca gcctcataga aaaataccgg
     2041 gatgcggtgg cgtttccaga tttcgttgcc gacccggcgg gcgagcgcaa cgcaatcatc
     2101 gagcgtaaca ccctcaatgg gaatgaaagg aacgacatcg gtcgcgccaa ctcgcggatg
     2161 agcgccttgg tggacggtca ggtcgatgag ttcagcagct ttgcctacgc cgagtagcgc
     2221 cgctgcggca acagcgtccg ggtcaccggc gagggttacg acgcagcgat tgtgatcggc
     2281 atccatttca cggtcgagga cgtacacgcc aggcacgctg ctcatggcgg caacaatggc
     2341 gtcaaccttc gcgggatcgc gtccttctga aaaattggga acgcactcaa tcaatcgggt
     2401 catatttgaa ttgtccggat cacgatggtg gagacctcag ttagcgctaa gcggtgattt
     2461 ggaataaacg attcggcccc gcttgatggt ggtatgacac gtgttcattc caaaataata
     2521 gggaatttca cggtagtcat ccacatcgaa gatggcgata tcagcaagtt taccaacctc
     2581 aagggagcca atctgttttt cgcggccgag tgaatatgcg ggattgatgg tcgccgcgga
     2641 aacagcttcg gctggggtca tgcgcaactg ggtgcacgcc agcgacagaa tcatcggcat
     2701 gcttacggtt gggctggtgc cgggattata gtccgtggcg agagcgacga tggctcctgc
     2761 gtcgatcaag tcgcgtgcgg gggcatattg ttttagcccc agatgaaaat tacagcccgg
     2821 cagcagtgtg gctacagttt tagactttgc gagtgcacgg atgtctgcgc gatttatttt
     2881 ctccagatga tcgcaactgg ccgcgccaag ttttacggca agctgtgtcc cgccggtgcg
     2941 cgaaagttgt tcagagtgga ttcgtggcgt caggccgtaa cgcttaccgg cttcgaggat
     3001 ctgtttggat tgcgcgaggt tgaatgcacc gcgctcgcag aaaacatcgc agtattcggc
     3061 caatttctgt gcggcgattt ctggaaggag tttgtcgatc aggagcgaga tgtacttttc
     3121 agcgcctgat ggacggctgc gaaattcggc agggattacg tgagcaccca ggaacgtgga
     3181 ggcgatatcc aaaggctgtt ctgccgaaag ctccttatgc aattttaaaa tttttagctc
     3241 accggccagg tcgagaccgt agccgctttt agcttcaatg gtggtggtac cgtaagcggc
     3301 gaactgtctc aaagccgaat gggcgcgttg ctttaatgtg tccgaggtcg cggcgcgtag
     3361 tttgttcaca gagtttaaga tgccgccgcc acgcttgg
//
LOCUS       DATCBB010000239         1045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_429501, whole genome shotgun sequence.
ACCESSION   DATCBB010000239 DATCBB010000000
VERSION     DATCBB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429501"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VII37_02670"
     CDS             complement(<1..672)
                     /locus_tag="VII37_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY5068654.1"
                     /translation="MIRIKTLRKELPPTVRLALPLVLAELGWMSMAIVDTIMVGHLPD
                     SATAMGAVSLGSNIFIVPALFGAGLLMGLDTLVSQAFGAGQREDCHRSLINSIYLSVA
                     LTPILAAPLWLLPNLLRSLHIEPAILALAIPYTNALTVGLFPLLLYFAVRRCLQAMNM
                     VRIVAFALITANIVNAVGNWIFVYGKWGAPAMGAVGSGWSTALARLYMATVLIVYLLW
                     YDHRHR"
     gene            complement(740..>1045)
                     /locus_tag="VII37_02675"
     CDS             complement(740..>1045)
                     /locus_tag="VII37_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068655.1"
                     /translation="KPIAKLSVLTFVDLQDLPHRQTDGKNTNELRMVAAIFDRNGKYI
                     GAIDRKVAVQWSDDKAGTQTAATFSFLLDFGAYRVRLVARDAESKRLFAQASVIQIP"
BASE COUNT          246 a          259 c          337 g          203 t
ORIGIN      
        1 gcgatggcga tggtcgtacc acaagagata aacgatgagg acggtagcca tgtacaggcg
       61 cgcgagcgct gttgaccatc ctgagccaac ggcacccatc gcgggcgctc cccatttacc
      121 atagacgaag atccagttgc cgacggcgtt gacgatattc gcggtaatga gcgcgaaggc
      181 cacgatgcga accatgttca tggcttggag gcaacgccgc accgcgaaat acagcagcaa
      241 tgggaaaaga ccgacggtta gcgcgttcgt gtagggaatg gcgagcgcaa ggatggcggg
      301 ctcgatgtgc aggctgcgaa gaaggttggg cagcagccac agcggtgcgg cgaggatggg
      361 agttagcgcg acgcttagat aaatactgtt gatcaacgag cggtggcagt cctcgcgctg
      421 gccggcgccg aacgcttgag ataccagggt gtcgaggccc atgagaagtc cggcaccgaa
      481 cagtgcgggg acaatgaaga tgttggagcc gaggctcacc gcgcccatgg cggtggcgct
      541 atcaggaaga tggccgacca tgatggtgtc tacgatggcc atgctcatcc agccaagctc
      601 cgctagcacc agcggcaggg cgagacgcac cgtgggtggc aattctttgc gaagagtttt
      661 aatgcgaatc atggaacaag ttgtgcggtg aagtttaagc ggaggcgcgc gaacaggcaa
      721 acgcgactaa attcctaagt cagggaattt gaatcacgga ggcttgcgcg aatagtcgtt
      781 tggattcggc atcgcgagcg accagcctga cgcggtaggc tccgaaatcc aacaggaaac
      841 taaaagttgc ggctgtttgg gttccggctt tgtcatcgga ccattgcacc gcgacttttc
      901 gatcaatcgc gccgatgtat ttgccattgc gatcaaaaat agcggcaacc attctcagct
      961 cgttggtgtt ttttccgtcg gtctggcgat gcggcaagtc ttggagatca acgaaagtca
     1021 gcacgctgag ttttgcgatg ggctt
//
LOCUS       DATCBB010000240         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_331963, whole genome shotgun sequence.
ACCESSION   DATCBB010000240 DATCBB010000000
VERSION     DATCBB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331963"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VII37_02680"
     CDS             <1..1028
                     /locus_tag="VII37_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5068656.1"
                     /translation="ALNGYDTARSVALIDQMRDRFATLPGVKAVGSSEISSLTGTDMG
                     ANITVEGGPQLPEDQEHVLYDPVSPAYFSAIGVPLVAGREFNSGDTSARSKVAIVNES
                     MAKRFFPKRDPVGSRFGFGSGNAVKPDIQIVGVVKDNKQDHVRSEMAPYIFLPYAQRD
                     KLSGMTFYVRTQQDPLLIADELRDSVRGLDPNLPVYDLKTFQRVVDEDLLAERLIAGL
                     SASFGVLAALLAAMGIYGVLAYLVIQRTREIGIRMALGAATRQVRALIYKEMSYMVIA
                     GALVGLPVAYALARLSESLLYGVRANNVGIYLVALTVVAVIAAAACYLPVRRATRVDP
                     MVALRYE"
     gene            1092..1802
                     /locus_tag="VII37_02685"
     CDS             1092..1802
                     /locus_tag="VII37_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5068657.1"
                     /translation="MMESNGTLIKMEGVKKVFYTDEVETHALSEIHLEIQKGQYISIA
                     GPSGCGKSTLLSILGLLDSPTDGNYWLNGQPVSKLTISERTRIRNREIGFIFQAFNLI
                     GDLTVYENVELPLTYRGTGTAERKQKVQRALERVGMAHRLRHYPSQLSGGQQQRVAVA
                     RALVGDPLILMADEPTGNLDSKNGEAVMELLRDLHRNGATICMVTHDERYANFAERNI
                     HLFDGRIVDETREVSVGA"
     gene            1845..>3882
                     /locus_tag="VII37_02690"
     CDS             1845..>3882
                     /locus_tag="VII37_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068658.1"
                     /translation="MDTVLQDIRYALRMLWKHRFATFICAGALALGMGANTAIFSMAE
                     AFLLHPVPLDEVNRLVAVVDSKPRQNIEKTSVAPATYLEWQEQSRSFDRLAAYTWDGV
                     NFTGDGTPQKIQDFQITANFFSTLGVQPHLGRDFLPEEGEPGKNQELILSYGLWERRY
                     AADPKILGKVVKVDGKPFTIVGVMGKGFDFPMSAEAWLPMAFDAKQRVERDSRPLWVI
                     GHLKNGVSFEQANAEMLTIAQHQAAAYPDAYKDWQLRVMPLGEFATGDLTRQYTLLLL
                     GAVGFVLLIACADVANVQFARVSGRQKELAVRAAMGASRTRIVRQLLIESVMLSLFGA
                     ALGVFLAQWILSVILSHMPPDIARYIAGWKTIRLDFGAFLFTFGVALASGLLSGIAPS
                     LMSSRTNISETLKESGRGSSSGRARHRLRSILVVAEVSLSLVLLVGAGLLVKGFNALL
                     TVNQQYHPESLLTLNLDLPQLQYPERSARETFHDRLLQNLSSIPQVQSVALATTVPYG
                     NGGGTDAQRFLIEGRPVEQREESRTAIMETVSPNYFAVMSMGLRNGRTFTESDGDETQ
                     PVAVISQSLASRHFTSENPLGKKVQVVGSKPETWLTVVGVVDDVRYNWIQKDVLPTLY
                     RPYRQNPTYYTTVVLRTTGEPPKFASAVRSKIAAIDPNLPIYEVKPLSQVITESI"
BASE COUNT          817 a          982 c         1164 g          919 t
ORIGIN      
        1 cagcgctgaa tggctacgac acggctcgca gcgttgcgct gatcgaccaa atgcgcgatc
       61 gttttgcgac gcttcctgga gtgaaggcgg taggttcttc ggagatttcc agtctgacgg
      121 gcacggacat gggagctaac attaccgtcg agggcggccc gcagcttcct gaggatcagg
      181 aacatgtcct gtatgacccc gtaagtcccg catatttttc tgctatcgga gtacccctag
      241 tggccgggcg cgaatttaat tctggggata cgagcgcacg gagcaaagtt gcgattgtca
      301 acgaaagcat ggccaagcgt ttttttccta aacgcgatcc ggtggggtca cgcttcggat
      361 ttgggtccgg gaatgcggtc aagccggaca ttcagatcgt cggggtggtc aaggataaca
      421 agcaggatca cgtgcgcagt gaaatggctc cctacatctt cttgccgtat gcgcagcggg
      481 acaaattgag tggcatgacc ttttatgtgc gcacgcagca ggatccgctg ctcatcgctg
      541 atgagttgcg cgacagcgtc cgcgggctgg atcccaatct gccggtctac gatttgaaga
      601 cctttcagcg tgtggttgac gaggatttgc tggccgaacg gctgatcgca ggattatccg
      661 cgagcttcgg cgtcttggca gcgctactgg cggccatggg tatctacggc gtactcgcct
      721 atctggtcat tcaacggact cgtgagattg gaattcgcat ggccctgggt gccgccaccc
      781 ggcaagtacg tgcgcttatt tataaggaaa tgagttacat ggttatcgcg ggcgcgctcg
      841 tgggattgcc ggtggcgtac gcgctggcgc ggctgagcga atccctgctt tatggtgtgc
      901 gcgctaacaa tgtggggatc tatctggtgg cgttaactgt tgtcgccgtg attgcggccg
      961 cagcctgcta tctcccggtg cggcgcgcca cgcgtgtcga ccccatggtg gccttgcgtt
     1021 atgaatagca gcggaactaa atcgggccag attcgtatca actggtgcgc agagaacttg
     1081 aaggagaaaa catgatggaa tcaaacggca ccctgatcaa gatggaaggc gtgaagaagg
     1141 tgttctacac cgatgaagtt gagactcacg cgttgtcgga aatccacctg gagatccaga
     1201 aagggcagta tatttcgatt gccggacctt ccggctgcgg caagtccacg ctgctttcta
     1261 ttctggggct gctggattcg cctacggatg gaaattattg gctcaacggt cagcccgttt
     1321 cgaagctgac tatcagcgaa cggactcgta ttcgcaaccg cgaaatcgga ttcatttttc
     1381 aagctttcaa ccttatcggc gaccttacgg tgtatgaaaa cgtcgagctg ccgctcactt
     1441 atcgcggcac cggcacggcg gaacgcaagc agaaagttca acgcgcactg gagcgcgtag
     1501 gcatggcgca ccgcttgcgt cactaccctt ctcaactttc tggaggtcag cagcagcgtg
     1561 ttgcggtggc gcgtgcgttg gttggcgatc ctttgatctt gatggctgat gagccgaccg
     1621 gaaacctgga ctccaagaat ggcgaagcgg tgatggagtt gttgcgcgat ctacatcgca
     1681 atggagccac tatctgcatg gtcacacacg acgagcgcta cgcgaatttt gccgagcgca
     1741 atatacattt gtttgacggg cgcattgtgg atgaaacccg ggaagtcagc gtaggcgcgt
     1801 aaattcttta ggccaaacat tgtttgccac tgaggcctta gttcatggac accgttctgc
     1861 aggatattcg ttatgcctta cgcatgcttt ggaagcaccg gttcgcgacc ttcatctgcg
     1921 ctggcgcgct ggcgctgggt atgggagcca ataccgcgat cttcagcatg gcggaagctt
     1981 tcttgctgca tcccgttccg ctggatgagg tcaatcggct cgtcgcggtg gtagattcga
     2041 aaccgcgcca aaacattgaa aagacttccg tcgctccggc tacttatcta gaatggcagg
     2101 aacaatcgcg ttcctttgac cggctcgcag cctatacctg ggatggtgtt aattttaccg
     2161 gggacggcac accacaaaaa attcaggatt tccagataac cgcaaatttt ttctcgacgc
     2221 tgggggtaca gccacatctt gggcgtgatt ttcttccgga agaaggggaa cccggaaaaa
     2281 atcaggagct cattctgagt tatggactct gggaacgccg ctacgccgca gaccccaaaa
     2341 ttctgggtaa ggttgtgaag gtcgacggaa aaccgttcac aattgttggc gttatgggga
     2401 agggttttga cttccctatg agcgcagagg cttggttgcc catggcgttc gacgcgaaac
     2461 agcgtgtgga gcgggacagt cgaccgttgt gggtaattgg ccatctgaag aatggggttt
     2521 cgtttgagca agctaacgcg gagatgctca ccatcgcgca gcaccaagca gccgcttatc
     2581 ccgacgcgta taaagattgg caacttcgcg tcatgccgct gggtgaattc gctaccggcg
     2641 atctcacgcg ccaatacact ttgctgctgc ttggcgcggt cggttttgtg ttgttgattg
     2701 cttgcgcgga cgtcgccaat gttcaatttg cgcgagtgtc cgggcggcag aaagaactcg
     2761 ccgtgcgcgc cgcgatgggt gcgagccgca cgcgcatcgt tcggcaactg ttgattgaga
     2821 gcgtaatgct gtcgcttttt ggcgcggcgc tcggagtttt tctggcgcag tggatactta
     2881 gtgtgatcct ttcccatatg ccgccggata tcgcgcggta catcgccggc tggaagacca
     2941 ttcgacttga ttttggcgcg ttcctgttca cgtttggtgt ggcgttggcg agtggattac
     3001 tttcgggcat cgcgccgtcg ctgatgagtt cgcgcacaaa tatttcggag actttgaagg
     3061 agagcggacg cggttcttcg tcgggacgcg cgcggcatcg cttgcggagt attttggtgg
     3121 tcgcggaggt ctcgctgtcg ctggtattgc tcgtcggcgc cggcctgctg gtaaagggat
     3181 ttaatgcgct gctcacggta aaccagcaat accacccgga atcgctgtta acactgaatc
     3241 tggatttgcc gcagttgcag tacccggaga ggtccgcgcg tgaaacgttt cacgaccggc
     3301 ttttgcaaaa tctttcgagc attccccaag tgcagtctgt cgcgctggct acgaccgtgc
     3361 cgtatggaaa tggcggaggc acggacgcac agcgattctt aatagaaggc cggcctgtcg
     3421 agcaacgcga agaatcgcgt actgccatta tggagacggt ttcgcctaat tattttgcgg
     3481 tgatgagtat gggcttgaga aatggccgga catttaccga atcggatggc gacgaaactc
     3541 agccggtcgc ggtaatcagc caaagtctcg cgagccgcca ctttacaagt gagaatccgc
     3601 taggtaaaaa ggttcaagtt gtagggtcca agccggagac atggctcacc gtggtgggag
     3661 ttgtggacga cgtgcgttac aactggatac aaaaggacgt gttgcctacg ctttacagac
     3721 catatcgtca gaatcctact tactacacta cggtggtttt gcgcaccact ggtgagccgc
     3781 caaagttcgc ttccgcagtg cgcagcaaga ttgcagctat agatccgaat ctgccgattt
     3841 acgaggtgaa gccgcttagc caggtaatta ccgaatcgat ag
//
LOCUS       DATCBB010000241         1333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_397028, whole genome shotgun sequence.
ACCESSION   DATCBB010000241 DATCBB010000000
VERSION     DATCBB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397028"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VII37_02695"
     CDS             complement(<1..1313)
                     /locus_tag="VII37_02695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY5068659.1"
                     /translation="MTLATFHSFCEALLVELDPGRIILDKCDHWILLRRNLARLRLEK
                     YRRLPDPGQFLNDFLEFFSRCQDELVSCEDFQAYADGFGAKLEAERAELDEDTYAERA
                     DQVALQQEIARAYGASEGLLREKNRASINSLITGAVELLQKDSALREKLRQRYRFILV
                     DEFQDTNIAQIELLDLLCAEPRNIFVVGDNDQAIYRFRGASFASFAIFEKRFARLKDS
                     DDPSRYRVTLTENYRSTPNILRVATHVIGQNERSDRFPPKMLTTSHPEGEKIRIAELA
                     NADDESRWVTGELQRLHRAGHRWKDFAVLYRQHAHRDQLVEELSRAKIPFVISKLSIL
                     EHPLVRDVVAYLRLIAAPYDDVACARVLAAPAWHMEPEDLVRLTQRASKKRGAALIDI
                     LQAPQTELAFDASQASVQVLLDFLLAQRKTMNRRSAKQILGSVID"
BASE COUNT          292 a          456 c          314 g          271 t
ORIGIN      
        1 caatcgatca ccgacccgag aatctgcttc gcgctgcgcc ggttcatcgt ctttcgctgc
       61 gccaaaagaa aatccagcag cacctgcacc gaagcttgcg acgcatcaaa cgccagttcc
      121 gtctgcggcg cctgcaaaat atcgatcagc gccgccccgc gtttcttaga agcgcgttgt
      181 gtcagtctca ccaggtcttc cggttccatg tgccacgccg gcgccgccag cacccgcgcg
      241 caagccacat cgtcataagg cgccgcgatc agccgcaaat acgccacgac atcccgcacc
      301 agcgggtgct ccagaatcga aagcttgctg atcacaaatg gaatcttcgc ccgcgaaagc
      361 tcttccacaa gttgatcgcg gtgcgcgtgc tgccggtaca acaccgcgaa atctttccac
      421 cggtgtcccg cgcggtgcag ccgttgcagc tcgccggtaa cccaccgcga ctcatcgtcc
      481 gcgttagcca gctccgcgat tcgaatcttt tctccctccg gatgcgaggt ggtcagcatc
      541 ttgggaggga agcggtcgct gcgctcgttc tgtccaataa catgcgtcgc cactcgcaaa
      601 atattcggcg tggaccggta attctccgtc agcgtcacgc ggtaccggct gggatcgtcg
      661 ctatctttca atctcgcgaa tcgcttctca aaaatggcaa agctcgcgaa cgaagctccg
      721 cggaatcggt aaatggcctg gtcgttgtcg cccaccacaa aaatatttcg cggctccgcg
      781 cacaataaat ccagcagctc gatttgcgca atattcgtat cctgaaactc atccaccagg
      841 ataaagcggt agcgctgtcg cagtttctcc cgcagcgccg aatctttttg cagcagctcc
      901 actgctccag taatcagtga attaatcgac gcccggtttt tctcgcgtag caatccctcg
      961 ctggccccgt aagcccgcgc aatttcctgt tgcagcgcca cctggtcggc ccgctccgcg
     1021 taagtatcct catccagctc cgcacgctcc gcctctagct tggcgccgaa cccatccgca
     1081 taagcttgaa agtcctcgca cgaaaccagt tcatcctggc accggctaaa aaattccaga
     1141 aaatcattca aaaactgccc cggatccggc agccgccggt atttctccaa acgcaaccgc
     1201 gcaaggttcc gtcgcaacaa aatccagtga tcgcatttat ccagaatgat ccgcccagga
     1261 tccagctcca ccaaaagcgc ctcgcagaag gaatggaacg tcgccaatgt cacatccttc
     1321 ccgcgctccc cca
//
LOCUS       DATCBB010000242         1619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_7724, whole genome shotgun sequence.
ACCESSION   DATCBB010000242 DATCBB010000000
VERSION     DATCBB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7724"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VII37_02700"
     CDS             complement(<1..919)
                     /locus_tag="VII37_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068660.1"
                     /translation="MKPRSHYEGDCRGGIFRSAPWLCGLAIVLCVSMVSSRARAAVEL
                     EPLEHTKSGDPATPHAVDQRTGVLETQDGLTLRLNADLGSVRIITLEAGAATAVRYTV
                     HIETDARAPQAQALLDKYVLRAKATPAGVEIEGALPPQAARAASSGAQFWVQFEIAVP
                     AGYSLDVKTEAGDIETQDIGGTATLVTQGGNIRTGRIGVGAMQNIAHHMHDVNAGDSI
                     HGSSKLRSEGGHIQVLDVAGNLNVFTGGGHINVGNITGDAALRSGGGHIRAGTIGGRA
                     DLDTDGGNITVGHAGSFVNVRTGGGQIDFG"
     gene            complement(943..>1619)
                     /locus_tag="VII37_02705"
     CDS             complement(943..>1619)
                     /locus_tag="VII37_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY5068661.1"
                     /translation="DEQLSKMAVAGINLAPWPNAAPGTVQLQLRAEQPMVLSGNVDDS
                     NMRRVLTYVVENGERFDAGVRLDCLDALKAQARDAQVRKALLLAARRDQNPAVRMKAL
                     EALKDSAADDTVREALLDALAHDANPGVRVEVVNMLVHSLEHESQDSNMVSFPKLAGA
                     AENSDDQSDEMQMTSSGESSAMFSDASVEKVIRTLEELQKRDPSRYVRLRSAAALRQI
                     GPREPQ"
BASE COUNT          326 a          514 c          444 g          335 t
ORIGIN      
        1 cgccgaaatc aatttgaccg ccgccggtgc gcacgttgac gaaacttccc gcgtgcccaa
       61 cggtaatgtt tccgccatcc gtatcgagat cggcgcgccc accgatggtg ccggcgcgaa
      121 tgtgaccgcc gccactacgc agcgcggcgt caccagtaat gttgccgaca ttgatgtgtc
      181 caccgccggt aaaaacgttc aagtttccag ccacatcgag aacctgaatg tgtccgcctt
      241 cgcttctcaa tttcgatgag ccgtggatag agtcgccagc gttgacgtcg tgcatgtgat
      301 gggcgatgtt ctgcatggcg cccacgccaa tccgcccggt gcggatattt ccgccctggg
      361 tgacaagcgt ggcggtgccg ccgatgtcct gagtttcaat gtcgcctgcc tcggttttaa
      421 cgtcgaggct gtatcccgca ggcacagcaa tttcaaattg cacccagaat tgcgcgccgg
      481 atgaagctgc tcgcgcggct tgcgggggca acgctccctc aatctccaca cccgcgggag
      541 tggctttggc gcgtagaaca tacttgtcca gcagcgcctg ggcctgtggc gcacgcgcat
      601 ccgtctcgat gtgcacggtg taccgcaccg cagttgccgc accggcttcc agcgtaataa
      661 tacgtacgga acctaagtcg gcgtttaacc gcaacgtcaa cccgtcctgg gtctcaagca
      721 caccggtgcg ttggtcgacg gcgtgcggcg ttgcaggatc gccagatttc gtatgctcga
      781 gaggctccaa ctccacggcg gctcgggctc gactggaaac catggagaca caaagaacaa
      841 tcgccaatcc gcagagccac ggcgcgctgc gaaaaatccc gcctctacag tcgccttcgt
      901 aatgggaacg aggtttcatg gatgaactga caccgctgat cactactgag gttctcgtgg
      961 gccaatctgt cgcaatgcag cggcgctacg caaccgcaca tagcgactgg gatcccgctt
     1021 ttgaagttct tccagagtgc gaatgacttt ttctacggaa gcgtccgaaa acatcgcgct
     1081 ggattcgccc gacgaagtca tctgcatctc atcggattgg tcatcagaat tttctgccgc
     1141 gcctgcaagt ttggggaacg aaaccatatt tgaatcttgc gactcatgtt ccagcgagtg
     1201 caccaacata ttcacaactt ccacgcgcac gcccggattc gcatcatgcg ccaaggcatc
     1261 aagcaaagct tctcgaacgg tgtcatccgc agcggaatcc ttgagcgctt ccagggcttt
     1321 catgcgtacc gcgggattct ggtcccttcg cgccgccaac agcagcgcct tacgcacctg
     1381 cgcatcgcgt gcctgcgctt tgagcgcgtc cagacaatcc agccgcacgc cagcatcgaa
     1441 gcgctcgccg ttttccacga cgtaggtaag cacccggcgc atattgctat catccacgtt
     1501 gcccgaaagc accatgggct gctccgcacg caattgcagt tgcaccgtgc cgggcgcggc
     1561 attcggccac ggcgcgagat tgattcccgc cacggccatt ttcgaaagct gctcgtcgg
//
LOCUS       DATCBB010000243         4673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_397078, whole genome shotgun sequence.
ACCESSION   DATCBB010000243 DATCBB010000000
VERSION     DATCBB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397078"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VII37_02710"
     CDS             complement(<1..315)
                     /locus_tag="VII37_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068662.1"
                     /translation="MDAQLKVISRAGIGEAIAKAELYRYLNEPEEAESICRDILALDS
                     GNQLARRLLGLSITDQFTGYAGDRYGEVAEIFQGLRDAYERAYYTGLLYERRAKVQLR
                     SGY"
     gene            complement(358..1104)
                     /locus_tag="VII37_02715"
     CDS             complement(358..1104)
                     /locus_tag="VII37_02715"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY5068663.1"
                     /translation="MAETVLRVFRGDASGGATVDYNVPVAPGMVVLDALHYIQGHLAP
                     DLAVRWNCKAGKCGSCSAEVNGRPRLTCKTRMDELPKDEPITVMPMKSFPKIKDLVTD
                     VSWNYRVNKKIPPFQPRRGADWKISQLDVDRVQEFRKCIECFLCQNVCHVLRDHDKKE
                     QFGGPRFFVRVAGLEMHPLDGANRSRLLKDELGLGLCNITKCCTEVCPEEIHITDNAI
                     IPLKERVVDEFYDPLTMLFRKMRGPSEKRN"
     gene            complement(1168..2880)
                     /locus_tag="VII37_02720"
     CDS             complement(1168..2880)
                     /locus_tag="VII37_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5068664.1"
                     /translation="MTTNYEVREHDVLIIGAGGAGLRAAIEALAQGASVGVVCKSLLG
                     KAHTVMAEGGIAAAMANVDPADNWKTHFRDTMRGGKLLNNWRMAQLHAQEAPDRVREL
                     EQWGALFDRTEKGEILQRAFGGHTFKRLCHVGDRTGLELIRTLQDRGVQQGVDCYMEC
                     TVTRLLMENGRFAGAFAYWRENGRFIVFKAKAVVIATGGIGKAWPITSNSWEYTGDGM
                     ALAYQAGAELMDLEFVQFHPTGMVWPPGVQGILVTEAVRGEGGILRNKLGERFMEKYD
                     PKRMELSTRDVVARSIYTEVREGRGTEHGGAYLDISHKPAEYVKKKLPSMYHQFKELA
                     DVDITKEPMEVGPTCHYVMGGIRVEAETGQSSMPGIFAAGEAAAGLHGANRLGGNSLS
                     DLLVFGRRAGMAAAAHAKQTAYASLDNAQMQDAEKELLAPFSNKGESPYAVHRDLQTV
                     MQSLVGIYRNAEDLERALNELQMLKARAAAASVEGSRMFNPGWHLMGELKSMLIIAEA
                     VTKSAMSRTESRGAHSRIDYPNLDTAWGQKHNIIVQEGGEMVRREDPIPPLPADLKEV
                     LAQE"
     gene            complement(2877..3635)
                     /locus_tag="VII37_02725"
     CDS             complement(2877..3635)
                     /locus_tag="VII37_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068665.1"
                     /translation="MAGTPTQLPQARLGATLRRDSWWTELLPVIILLGGFGVYATLRA
                     FEGAYYSWGPYLSPFYSPLIDPQHHWWPLSPALLVLAGPLGFRATCYYYRKAYYRAFF
                     LDPPACAVGEPANRKYRGETAFPFILQNVHRYFLYLAILFLAFLWHDAIKGFFFDGQF
                     GIGVGSLILLLNVILLSIYTLSCHSLRHLAGGKMDCFSCASFGTTRAAAWRGFSFLNE
                     RHMQFAWASLISVGSADLYIRLVASGIIRDLRIL"
     gene            complement(3750..4028)
                     /locus_tag="VII37_02730"
     CDS             complement(3750..4028)
                     /locus_tag="VII37_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068666.1"
                     /translation="MMTSEATRRAPMLPLRQLVDRYRALAGDFGTPVPLTAFNLSRAE
                     TEQLFSSYDEDYHISRFFHFDDLDGMTFTINAEPVTHVALDAEIETIL"
     gene            4245..>4673
                     /locus_tag="VII37_02735"
     CDS             4245..>4673
                     /locus_tag="VII37_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5068667.1"
                     /translation="MPGATAQTREGAPRPADVLHQLNEAIEDLVQRVSPTVVQILVTG
                     YGAAEETERGQTDLLVGRRRGIGSGVIVDPEGYIITNAHVVNGAQSIEVLVPPPPAPT
                     ASGAATDGPQSRSFHARVVGTSREIDLAVIKIDAHALPALS"
BASE COUNT         1027 a         1331 c         1313 g         1002 t
ORIGIN      
        1 atagccgctg cgtagttgca ccttggcgcg gcgctcgtat agcaaaccgg tgtaataagc
       61 gcgttcatag gcatcgcgca ggccttgaaa tatttcagcc acttcgccgt agcgatcgcc
      121 ggcgtagccg gtgaattgat cggtgatcga caatcccaac aaacggcgcg cgagttgatt
      181 cccggaatct agggccagaa tatcgcggca gatcgattcg gcttcttccg gttcgtttaa
      241 atagcgatat agctccgctt tggcgatggc ttcaccgatg cctgctctgg aaataacttt
      301 gagttgagcg tccatttcaa cctcccgaac tgcagacaca atactctttt agccaggcta
      361 attgcgtttt tcgctgggcc cccgcatttt gcgaaatagc atggtcaaag gatcgtaaaa
      421 ttcgtccact accctttctt tcaacggaat gatggcgtta tccgtgatgt gaatttcttc
      481 ggggcaaacc tcggtgcagc atttggtgat gttgcaaagg ccgagaccca gctcatcttt
      541 cagcaagcgc gaacgatttg cgccgtcgag cggatgcatt tccagtccgg cgacccgcac
      601 aaaaaaacgc ggtccgccga actgttcttt tttgtcgtga tcgcggagca cgtggcaaac
      661 gttctggcac agaaaacatt cgatacactt gcgaaattcc tgcacgcggt cgacgtccag
      721 ctgcgagatt ttccagtccg ccccgcgtct tggttgaaat ggcggaattt ttttgttcac
      781 gcggtaattc caggaaacat cggtgaccag gtctttgatt ttcgggaagg acttcatggg
      841 catcacggtg atgggctcat ctttgggaag ctcatccatg cgggttttgc aggtcagccg
      901 agggcggccg tttacttccg cggaacagga accgcacttc cctgctttgc aattccagcg
      961 caccgcgaga tccggcgcga gatgcccctg aatataatga agcgcgtcga gcaccaccat
     1021 ccccggcgcg accggaacgt tgtagtccac cgtcgctccg ccgctggcat cgccgcggaa
     1081 cactcgcaag actgtttcag ccatcgctga tttctccatt ccggtgctca tcgccgcaaa
     1141 ttacagtcgt gaaatttgga tgccatgtta ctcctgcgcc aaaacttctt ttaagtcagc
     1201 cggcaggggc ggaatgggat cttcgcgccg aaccatctcg ccgccctcct gcacgatgat
     1261 gttgtgtttc tgcccccatg cagtatcgag gttcggatag tcaatgcggc tatgcgcgcc
     1321 tcgactttcg gtgcgggaca tggcgctttt cgtcaccgcc tcggcaatga tgagcatgga
     1381 tttcagctcg cccataaggt gccaacccgg attgaacatc cgcgaccctt ccacgctggc
     1441 cgctgccgcg cgagctttta acatttgcaa ctcgttcagc gcgcgctcca aatcttccgc
     1501 gttgcgataa atgcccacga ggctttgcat cacggtttgc aaatcgcgat gaaccgcgta
     1561 agggctttcg cctttgtttg aaaatggggc gagcaattcc ttttcggcgt cttgcatttg
     1621 cgcgttgtcc agcgaggcat aggcggtctg tttggcatgc gcggccgcag ccattccggc
     1681 acgccgcccg aacaccagca aatcggacag cgaattgccg cccaggcgat ttgctccatg
     1741 caaacctgct gcagcttcac cggcggcaaa aattcccggc atggaggact gccccgtttc
     1801 cgcttcgacg cgaatgccgc ccatcacgta atggcacgtc ggaccaactt ccatgggctc
     1861 tttggtgatg tccacatcgg ctagctcttt gaactggtga tacatgctgg gcagcttttt
     1921 ctttacgtat tcggcgggct tatgagagat gtccagatac gcgccgccgt gttcggtgcc
     1981 gcggccttcg cgcacttcgg tataaatcga acgcgccacg acgtcgcgag tggaaagttc
     2041 catgcgcttg ggatcgtatt tctccatgaa gcgctcgccc aatttgttgc gcagaatgcc
     2101 gccttcgccg cgcactgctt cggtgaccag aattccctgc actccaggag gccacaccat
     2161 cccggtggga tgaaactgca caaattccag gtccatgagt tctgcgccag cttggtaagc
     2221 cagcgccatg ccgtcgccgg tgtattccca ggagttggat gtgataggcc aggccttgcc
     2281 gatgccgccg gtggcgatga ccaccgcttt ggctttgaaa acgatgaagc ggccattctc
     2341 gcgccagtac gcgaaggcgc ccgcaaagcg gccgttttcc atcaacaggc gcgtgacggt
     2401 acattccatg tagcaatcca cgccctgctg cacaccgcgg tcttgcaggg tgcgaattag
     2461 ttcgaggcca gtgcgatcgc cgacgtggca caagcgcttg aaggtgtgac cgccaaaggc
     2521 acgctgcaaa atctcaccct tttcggtgcg atcaaaaagc gcgccccatt gttccagttc
     2581 acggacacga tctggagctt cctgcgcgtg caactgcgcc atgcgccagt tgttgagcaa
     2641 ttttccgccg cgcatggtat cgcggaagtg cgttttccaa ttgtcggcgg gatctacgtt
     2701 ggccatggct gcggcgatgc cgccttcggc cattaccgtg tgggctttgc cgaggagtga
     2761 tttgcagacc actcctaccg acgcgccttg cgccaaggct tcgatggccg ctcgtagtcc
     2821 cgcgccacca gcgccgatga tcaatacgtc gtgctcgcga acctcgtaat ttgtggtcac
     2881 agaatcctca ggtcgcgaat gatgccgctg gccaccagtc ggatgtacaa atcggccgag
     2941 cctacggaga tcaagctggc ccacgcgaat tgcatgtggc gctcattcag gaaggagaag
     3001 ccgcgccagg cggccgctcg cgttgtgccg aaagatgcgc aggaaaagca atccatcttg
     3061 cctcctgcca aatggcgcag cgagtggcac gacagcgtgt aaatggaaag caagatcacg
     3121 ttcagcagca gaatcaggct gcccactcca atgccaaatt gcccgtcgaa gaagaaacct
     3181 ttgatggcgt catgccacag gaaagccagg aaaagaatcg cgagatacag aaaatagcga
     3241 tgcacatttt gcagaataaa tggaaaggct gtttcgccgc gatacttgcg gttggccggc
     3301 tctccgacgg cgcacgccgg aggatcgaga aaaaacgcgc gatagtaagc tttgcggtag
     3361 tagtagcagg tagcgcgaaa tcccaaggga cccgccagca ccagaagcgc gggagagagc
     3421 ggccaccaat gatgctgcgg atcgatgagc ggggaataaa aaggcgaaag ataaggtccc
     3481 catgaatagt aagcgccttc gaacgcgcgc aatgtggcgt aaactccaaa gccgccgagc
     3541 agaatgatta ccggtaaaag ttcggtccac cacgaatcgc gacgcaacgt agcgccgaga
     3601 cgcgcttgcg gcaactgagt tggcgtgcca gccatgcagg tacctccgcg tgcagtagcc
     3661 tatgcaaaaa actcaatttt tcaaacgaaa ttatgtaggt gcgcagaaat aagggaataa
     3721 aacctcgagg gacagatcgc atcaaacact tacaaaatgg tttcgatctc ggcgtccaac
     3781 gcgacgtggg tcacgggctc ggcgttaatc gtaaaggtca ttccatcgag atcgtcgaaa
     3841 tgaaaaaaac ggctgatgtg gtagtcctcg tcataactgg aaaaaagctg ctccgtctcg
     3901 gcgcggctta gattaaatgc cgtgagcggt acgggagtgc caaaatcacc ggccagcgcg
     3961 cggtagcggt caacgagttg tcgcagtggc agcatgggag ctcgtcgggt ggcctcggag
     4021 gtcatcatcg ctctcctttg gccgagtgta ctgctaccct tgcgattaat aaatagccca
     4081 gaatgccgct ataatgcggt gcccaaacat tggctggcag tcggggaacc caacgtcgtg
     4141 agaaagagaa agataccaac atgagtacgt ttctcaactt tttcctcaga cgataccgtg
     4201 cggcctcatg cctgctggtt ctctcattct tcgggctctc gtcattgccg ggcgcaacag
     4261 cacaaacgcg cgagggcgcc cctcgtcccg ccgacgtgtt gcatcaactg aatgaagcca
     4321 tcgaagatct ggtgcagcgg gtatcgccca cggtagtgca aatactggtc accggatatg
     4381 gggctgccga agaaaccgag cgcggtcaaa cggacttgct ggtggggcgc cgccgaggca
     4441 tcggctcggg ggttatcgtc gatccggaag gctacatcat tacgaatgct cacgtggtga
     4501 acggcgcaca aagcatcgaa gtgctcgttc caccgccacc agctcccacg gcgtccggag
     4561 ctgcgaccga cgggcctcag tcaagaagct tccacgcgcg cgtcgtggga acttcgcggg
     4621 agattgatct cgcagtgatc aagattgatg cgcatgcgtt gccggcgcta tcg
//
LOCUS       DATCBB010000244         1339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_7741, whole genome shotgun sequence.
ACCESSION   DATCBB010000244 DATCBB010000000
VERSION     DATCBB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7741"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VII37_02740"
     CDS             <1..893
                     /locus_tag="VII37_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068668.1"
                     /translation="NFQAAVDKADVLPQFDESARLTFKQRLGFSYIRLGQGAKAEQTF
                     RELIAAFTRVSGPESPHVLRVRLNLAQAFMIQNKHAEAVQEATAIYPEFVSRLGPDHE
                     LTMQLLATKAQSEGSLERWDDAIRDDLAIHELAVRKQGPLSFFAVATLSDAALAQCRA
                     GRYRDGESNARKAYDSSSKAFGARAALTGAAGDTLANCLIGLGKLNEAATLLQQIDVP
                     AVAQLTGDPDWGAGVSLAQAEIAYRRGNYDTARVYLKTAEPVFTRKDQEPFQKRAFES
                     LKAALEKPLSTSLYHGNNER"
     gene            complement(900..1208)
                     /locus_tag="VII37_02745"
     CDS             complement(900..1208)
                     /locus_tag="VII37_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068669.1"
                     /translation="MDTVKKLLPSLAIAGGLLLGAALPQLQAEDIDRCQRRVIHAEHD
                     LHRAVERYGRNSARANHERRELHAARERCWRERHRWWDEHEHRWHTERDWDEHDHDRD
                     "
BASE COUNT          277 a          400 c          376 g          286 t
ORIGIN      
        1 acaacttcca ggcggcagtg gacaaggccg acgttcttcc ccaattcgac gagagcgcgc
       61 gtttgacgtt caagcagagg ctgggatttt cctatatccg cctcggccag ggagctaagg
      121 cggaacaaac gttccgcgaa ctcatcgccg cttttactcg tgtctcaggc cccgagagtc
      181 cccacgtttt gcgggtccgg ttgaatctgg ctcaggcctt catgattcag aacaagcacg
      241 ccgaggcggt gcaagaggca acagctattt atcccgagtt tgtctcgcgg ctgggcccag
      301 accacgagct gaccatgcaa ttgttggcga ccaaagcaca gtctgaggga agcctggaac
      361 gttgggatga cgcgattcga gatgatttgg ctattcacga actggcagtt cgcaagcagg
      421 gcccactgtc atttttcgcg gtggccacgc tttccgatgc agctctggct caatgtcgcg
      481 ccgggagata tcgcgacggt gagtccaacg cgcgcaaagc gtatgactca tcgtcgaaag
      541 cgttcggagc gcgcgccgca ctcaccggtg ccgccggcga cacattggcc aattgcctga
      601 ttggtttggg caagttgaac gaagccgcta cgctcttgca acaaatcgac gtgccagcgg
      661 tcgctcaact taccggcgat cccgattggg gcgcgggcgt atccctggcc caagccgaaa
      721 tagcatatcg ccgcggcaat tacgatactg ctcgcgttta ccttaagacc gcagagccgg
      781 tttttacccg gaaagatcag gagccctttc agaagcgcgc gttcgaatct ttgaaagccg
      841 cactggaaaa gcccctatct accagcctct accacggaaa taatgaacgc taaggctgct
      901 tagtcgcgat cgtgatcgtg ctcgtcccaa tcgcgttccg tgtgccagcg gtgctcgtgt
      961 tcatcccacc agcgatgccg ctcgcgccaa caacgctctc gcgccgcgtg taattcgcgg
     1021 cgctcgtgat tcgcgcgcgc actattgcgt ccatatcgtt ccaccgctcg gtggaggtca
     1081 tgctcggcgt gtataacgcg gcgctggcat cgatcaatat cctccgcctg cagttgtggc
     1141 agcgccgccc ccagcaaaag tcctccggca atagccagcg agggcagcaa cttttttacg
     1201 gtgtccattc aacagcctcc tgccgtgatt tttgaaaagg cggaactagg atgctaaagc
     1261 ccaggcagag tttgtgcgct atgttctttt actgttgctc gcggaggaac tggctgccaa
     1321 ttcccgaaac aggccgagc
//
LOCUS       DATCBB010000245         1273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_137766, whole genome shotgun sequence.
ACCESSION   DATCBB010000245 DATCBB010000000
VERSION     DATCBB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137766"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VII37_02750"
     CDS             <1..773
                     /locus_tag="VII37_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068670.1"
                     /translation="ICASIMSKKMAEGLDALVLDVKTGSGAFMRREEDAVRLAGVMIE
                     TGKGMGKKVVALITDMDQPLGRAAGHSNEVIECIDVLRGKGPADVRELSVELSAWMFY
                     LGEKTSSVEEGRKLAETMIASGKGLEKFRQCIRLQSGDERVIDKPDLLPAASSHVIVA
                     SPVRGNVAATNCEQFGVALAMLGGGRETKEDKIDHAVGLEFHKRIGDHVEKGEPLATI
                     HYNSGAKLAEAKRLVVESFVIGEEPAAVKLLIRRILGA"
     gene            848..>1273
                     /locus_tag="VII37_02755"
     CDS             848..>1273
                     /locus_tag="VII37_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HEY5068671.1"
                     /translation="MGRFVGVLGLLSMLGLAYIFSTDRKAIRLKTVLWGFGLQLTFAL
                     FVLRFSAGRRIFEFAGYYVNLLLSYAFVGSQFVFGELGMKDSPQHFIFAFQVLPTIIF
                     IAAFFALLYHIGVMQVLIRGAAWLMTRIMGASGAESLNVA"
BASE COUNT          275 a          300 c          422 g          276 t
ORIGIN      
        1 tgatctgcgc gtcgatcatg agcaagaaaa tggcggaagg gctggacgcg ctggttctgg
       61 acgtcaaaac cggctccggc gctttcatgc ggcgcgaaga agacgcggtg cggcttgcgg
      121 gagtgatgat tgaaaccggg aagggaatgg gcaaaaaagt tgtcgcgctg atcacggata
      181 tggaccagcc gctggggcgg gccgcggggc attcgaacga agtgattgag tgcatcgacg
      241 ttcttcgagg caaggggccg gccgatgtcc gcgagttgag cgtggagctg tccgcctgga
      301 tgttttatct aggcgaaaag acgagcagcg tggaagaggg acgaaaatta gcggagacca
      361 tgatcgccag cgggaagggg ctggaaaaat tcaggcagtg cattcgccta cagagtggag
      421 acgagcgcgt tatcgacaag cccgatttac tgcccgctgc gagttcgcat gtaatcgtgg
      481 cgagccctgt gcgcggaaat gtggcggcaa cgaattgcga gcaatttggc gttgcgctgg
      541 ccatgctggg cgggggccgg gagaccaagg aagacaagat cgatcatgcg gtcgggctgg
      601 aatttcacaa gcgcatcggt gaccacgtag aaaaaggcga accgctggcc acaattcatt
      661 acaatagcgg cgcgaagctg gccgaagcga aacggctggt ggtcgagagt tttgtcatcg
      721 gcgaagagcc tgccgcggta aagctgttga tacgccggat tctgggcgca taacgactcg
      781 cggcggatgc agcgggattc gcgccgaaaa gcaccaagtc gcacagcagc ggaaatgagc
      841 gccgaagatg ggaagatttg tcggagttct gggcctgctg agcatgctgg gactggcata
      901 tatattttcc accgatcgca aagccattcg tttgaagacc gtgctgtggg gattcggctt
      961 acagctaacc ttcgctcttt ttgtgttgcg tttttctgcc gggcgcagaa tttttgaatt
     1021 tgccggatac tacgtcaacc tgctgttgag ttacgccttt gtcggctcgc aatttgtttt
     1081 tggcgagctg ggcatgaagg attccccaca acattttatt ttcgcatttc aggtgctgcc
     1141 caccattatt tttatcgccg catttttcgc gctgctgtat cacatcgggg tgatgcaggt
     1201 gttgattcgc ggcgcggcgt ggctgatgac ccgcatcatg ggcgcgagcg gcgcggaatc
     1261 attgaacgtg gcc
//
LOCUS       DATCBB010000246         1710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_397187, whole genome shotgun sequence.
ACCESSION   DATCBB010000246 DATCBB010000000
VERSION     DATCBB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397187"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1271)
                     /gene="mgrA"
                     /locus_tag="VII37_02760"
     CDS             complement(279..1271)
                     /gene="mgrA"
                     /locus_tag="VII37_02760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEY5068672.1"
                     /translation="MSYSPATTRYDSPSFYRHCGRSGIKLPLISLGLWHNFGAKDSYE
                     NARAIALRAFDLGITCFDLANNYGPPGGSAEETFGRILREDLDKWRDEMIITTKAGYD
                     MWPGPYGEWGSRKYLLSSLDQSLKRMGLPYVDIFYSHRPDPDTPLEETMGALAHAVHS
                     GKALYAGISNYKPAETAQAAKILRELGTPCLIHQPRYSMLDRWVEPQLLATLEAEGIG
                     CTVFSPLAKGILTDRYFKGIPADSRAGHDQRFLRPSDITDAVLAKTKKLNDLAQSRGQ
                     TLAQMALAWVLRYKVVTSALIGASKVSQVDDCVGATRNLQFTGEELAQIETILA"
     gene            1521..>1710
                     /locus_tag="VII37_02765"
     CDS             1521..>1710
                     /locus_tag="VII37_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068673.1"
                     /translation="MNNPDTYSVDVPVIELDAQSRGRFIARTYNHLFAAICAFTLIEI
                     GLFQSGLAESIARAMLGTS"
BASE COUNT          355 a          502 c          479 g          374 t
ORIGIN      
        1 cggcccgcgc tcgcgggaaa actagggagt gtttttataa tcagtctgcg cgttaagcgc
       61 ctgaggcgcg ccagatagta gcccacggta aaaccgtggg gcggctgacc aacggatttt
      121 aaagctccgg atggggcggc agaaaatcga cccgcattca tttatttcgc cccatccggg
      181 gctgggtttg ccaaaatcct tttacccacg gttgcaccgt gggctactac cgtccgctgc
      241 tccgcacctt gatattgatt ttgaagacct gctccaaact acgcaagaat ggtttcgatc
      301 tgcgccagct cttcgccagt gaactgcaga ttccgcgtcg cgcccacgca atcatccacc
      361 tggctcacct tgctcgcgcc gatgagcgcg ctggtcacca ctttgtaccg caacacccac
      421 gccagcgcca tctgcgcgag cgtctgcccg cgcgactgcg ccaggtcgtt taactttttt
      481 gtcttcgcca gcactgcgtc ggtgatgtcc gagggacgga gaaatctttg gtcatggccc
      541 gcacgcgaat cggcggggat gcccttgaaa taacggtcgg tcaaaatgcc tttcgccagc
      601 ggcgaaaaaa ccgtgcagcc gatcccttct gcttccagcg tggcgagcaa ctgcggttca
      661 acccagcgat ccagcatgga gtagcgtggt tgatggatga gacacggcgt gccgagttcg
      721 cgaaggatct tcgccgcctg cgccgtttcc gccggtttat aattggatat gccggcgtaa
      781 agcgctttgc cgctatggac agcgtgcgcg agcgcaccca tcgtttcttc cagcggcgtg
      841 tcggggtcgg gacgatgcga ataaaaaata tccacatacg gcaatcccat gcgcttgagc
      901 gactggtcaa gcgacgacaa cagatatttt cgcgaccccc attcaccata cgggcccggc
      961 cacatatcat agcccgcctt ggtggtgatg atcatttcat cacgccattt gtccaggtcc
     1021 tcgcgcagga tgcgaccgaa ggtttcctcc gccgagcctc ccggcggccc gtaattgttc
     1081 gccaggtcaa aacaagtgat gcccaggtca aaggcacgca gcgcaatcgc gcgggcgttc
     1141 tcgtaagaat ctttcgcgcc gaaattgtgc cagaggccga gtgaaatgag cgggagcttg
     1201 atgccggagc ggccgcaatg gcggtaaaaa ctgggcgaat cgtaacgagt ggtagcgggt
     1261 gaatagctca tgccagcagc ataattgagg taagattagg cttcgataaa aaaattacaa
     1321 tccaccgtag cggcgtgtcg gcagaccgcc gcattggttt ggaaaattga actgtccggc
     1381 tttctgccga aagccgctac gccagttttc gcaggcgttt tcgcaggcgt attgacacga
     1441 ctgccggggc ggttaaactt gtccggtggt tcggcgggac gacgggacag tcgcctcgtt
     1501 attttaatcg cctgaaaact atgaataatc ctgacaccta ttcagtggat gttccggtca
     1561 ttgaactgga cgcccaatcg cgcggacgtt ttatcgcgcg cacctacaat catctgttcg
     1621 ccgccatctg cgcgttcacg ctcatcgaga tcggcctgtt ccagtccggc ctggccgaat
     1681 ccattgcccg cgcgatgctc ggcacttcct
//
LOCUS       DATCBB010000247         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_397227, whole genome shotgun sequence.
ACCESSION   DATCBB010000247 DATCBB010000000
VERSION     DATCBB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397227"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VII37_02770"
     CDS             <1..729
                     /locus_tag="VII37_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5068674.1"
                     /translation="PGMTSLEQLAEVRSTQKSTQRIYSIMYSERLENRATVRAAEIVR
                     AGGIGRVLQTIGTGPHRMNPSQRPPWFFERERYGGVLCDIGSHQFDQFLYFTNSTKAE
                     IVASQVGNLNHPQYPGLEDFGDVMLRGDHGTGYIRVDWFTPDGLATWGDGRLFVLGTD
                     GYLEIRKNVDIAGRNLGSHLFQVDQKGTRYIDCQDVPLPYGEQLINDVLDRTDTAMNQ
                     EHCFYAMELALTAEKQAQRIDRKT"
     gene            complement(770..>1542)
                     /locus_tag="VII37_02775"
     CDS             complement(770..>1542)
                     /locus_tag="VII37_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068675.1"
                     /translation="TQKSLIHIPAWDLNWQAVFRYEAHVELPAGTVISMRYRYDNSDE
                     NIRNPNVPPKRVVGGNRASDEMAHLWLQVLPRSNPGNTGDPRRILQEAMARHNVDKNP
                     ADFEAHYNLGAMLQGRGDLGAAEKQYALALSIRPEDATVNNAIAGAKLAAGHPDAAVT
                     YLQTALRSRPDYFDAHYNLGTALAMQNNFAAAVEEFRAAVRLNTADANAEANLGAALA
                     ELGNWKEAREHLEKALAINPALANARDNLEQVKRELPQ"
BASE COUNT          335 a          431 c          416 g          360 t
ORIGIN      
        1 cctggcatga cttcgctcga acaactcgcg gaagtgcgaa gcacgcaaaa atcgacccag
       61 cgaatctact ccatcatgta cagcgagagg ctggaaaatc gcgccacggt tcgcgccgcg
      121 gagatagtca gggccggggg aatcggccgc gtgctgcaaa ccatcgggac cgggccgcat
      181 cgcatgaatc cttcgcaacg gcctccgtgg ttttttgagc gcgagcgata tggcggtgtc
      241 ctttgcgaca ttggctccca ccagttcgac cagttccttt attttacgaa ttcaacaaaa
      301 gctgaaattg ttgcgtcgca ggtcggcaac ctgaaccacc cgcagtaccc gggtctggaa
      361 gattttggcg atgtgatgct gcgaggagat cacggaacag gttacatccg cgtcgactgg
      421 tttacgcccg acggtctcgc gacctgggga gacggacgac tttttgtgtt ggggacggat
      481 ggctacctgg aaattcggaa gaatgtcgac atcgccggaa gaaatctggg cagccatctt
      541 tttcaggtag atcagaaggg cacccgttat atcgattgtc aggacgtgcc acttccgtat
      601 ggagaacaac ttatcaacga tgtgctggat cgcaccgaca cggccatgaa tcaggagcat
      661 tgcttctacg ccatggaatt ggcgctaacg gccgaaaaac aagcgcaacg gatcgatcga
      721 aagacctagt cgctggcgta atcagtcctg gcgttcgtca gtcgtggggt cattggggaa
      781 gttcgcgttt tacttgctct agattgtcac gcgcatttgc gagcgctgga ttgatggcca
      841 gcgccttttc caaatgctca cgcgcttctt tccagtttcc taattcagca agcgcagctc
      901 cgagatttgc ttccgcattg gcatccgctg tattcagtcg cactgccgcc cgaaattctt
      961 ccaccgcagc cgcgaaatta ttctgcatgg ctagtgcggt tcccagatta tagtgcgcgt
     1021 caaaataatc aggcctggaa cgaagcgctg tctgcagata ggtcactgcc gcatcgggat
     1081 gacccgctgc tagcttcgcg cccgcgatag cattattcac cgtggcatct tcaggacgga
     1141 tgctcagcgc caacgcgtac tgtttttccg cagcccctaa atcgccgcgc ccttgcaaca
     1201 tcgcgccgag attgtagtgc gcctcaaagt ccgccggatt tttatcgacg ttgtgtcgcg
     1261 ccatggcctc ttgcaaaatt cttcgtggat ccccagtgtt cccaggattg cttcgcggca
     1321 aaacttgtag ccacaaatgc gccatttcat cgctggcacg attgccacct accacgcgtt
     1381 tcggcggcac attgggatta cgaatgtttt catcggaatt atcgtagcga taccgcatgg
     1441 aaattaccgt tccggccggc aattccacgt gcgcctcgta gcgaaagact gcctgccagt
     1501 tgaggtccca cgccggaata tgaatcaagg atttctgcgt tc
//
LOCUS       DATCBB010000248         1306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_364441, whole genome shotgun sequence.
ACCESSION   DATCBB010000248 DATCBB010000000
VERSION     DATCBB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364441"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1306
                     /locus_tag="VII37_02780"
     CDS             <1..>1306
                     /locus_tag="VII37_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5068676.1"
                     /translation="AVAIPTSIIATFTLMRAMDFSLNNMTLLALTLAVGIVIDDAIIV
                     LENIFRFMEEKGKDRATAAVQATREIGLAVMATTLSLVIIFLPIAFMSGYARKYVNSF
                     GWTMAMAIMVSLLVAFTLTPMMSSRLLKVGAKEKEQHAHGFMHSIEEFYLKLLRWSLA
                     HRRIILVIYVVTFASTFGLYHLVGRDWIPADDQAELMSSFTLPEGTSLEKTTQMAQDM
                     AGRVVALPEVAFVQTLTHGPTNHAHLFIGLVPRTERKRTHEQMAGVVRGILGSYRNIT
                     YNVRLPSVLGGETYFPIAAVIRGPEIDRLAEISKKIANRMRQFPELVDVNPSLNLNTP
                     ELQVKLDRQRAADIGVRMMDVSDSVRLFYSGEDEITRFKEGSEQYPVVMQLLPEQRDN
                     PEVLASMMVPSTKLGQVRLENVASIVRGAGPATLWRYNREFQV"
BASE COUNT          278 a          368 c          365 g          295 t
ORIGIN      
        1 gcggtggcca ttccgacttc tattatcgcg accttcacgc tgatgcgcgc gatggatttt
       61 tcgctgaaca acatgacttt gctggccctg acactggcgg tcggcattgt gatcgacgac
      121 gccatcatcg tactggaaaa tatttttcgg ttcatggaag agaaaggcaa agaccgggcc
      181 actgccgcgg tgcaggccac gcgcgaaatt gggttggcgg tcatggccac cacgctgtct
      241 ctggtcatta tttttctacc tatcgcgttc atgagcggtt acgcgcgcaa gtacgtgaac
      301 tcatttggct ggacgatggc catggccatc atggtgtcgc tgttggtagc tttcacgctc
      361 acgccgatga tgagctcgcg cctgctgaaa gttggcgcca aggaaaaaga acagcacgca
      421 cacggcttca tgcacagcat cgaggagttt tatctgaaac tcctgcgctg gtcgctggca
      481 catcgacgca tcattttggt gatttacgtg gtgacgtttg cttctacttt cggcctctac
      541 catcttgtcg gccgcgactg gattcccgcc gacgaccaag ccgagttgat gagctctttc
      601 acacttcctg aagggacttc gctcgaaaaa acgacacaaa tggcacagga catggctggc
      661 cgggtcgtgg cactgccgga agtcgctttc gtgcaaacgc tgacgcacgg accaacgaat
      721 cacgcgcatc tcttcatcgg attggttccc cgcaccgaac gtaaacgcac gcacgaacaa
      781 atggccggtg tggtgcgcgg cattctcggc agctatcgca acatcactta caacgttcgg
      841 ttgccttccg tcctcggcgg cgaaacgtac ttcccgatcg cggcggtaat tcgcgggcct
      901 gaaatcgacc gcttggcgga gatcagcaaa aaaatcgcca atcgaatgcg gcagtttccg
      961 gagctggtgg acgtgaatcc cagcctcaac ctgaacactc ctgaattaca ggtaaagctc
     1021 gatcgccaac gcgctgcgga tatcggtgtg cgcatgatgg atgtgtccga ctcggtgcgg
     1081 cttttttatt ccggggaaga cgagatcact cgctttaagg aaggctccga gcaatacccg
     1141 gtggtcatgc aattgctccc cgagcagcgt gacaatcccg aggtgctcgc cagcatgatg
     1201 gttccttcca caaagctcgg gcaggtgcgc ctcgaaaatg tggcttcgat tgttcgcgga
     1261 gcggggcccg ccacgctgtg gcgctataac cgcgaatttc aagtca
//
LOCUS       DATCBB010000249         3797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_202497, whole genome shotgun sequence.
ACCESSION   DATCBB010000249 DATCBB010000000
VERSION     DATCBB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202497"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="VII37_02785"
     CDS             <1..1490
                     /locus_tag="VII37_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEY5068677.1"
                     /translation="TKDVAYEPRPTQFRIDLRLLARAMEIRLPGRIARVPGSALVGGM
                     AFSFRVWELFVLTLALQIGMLPVLASAFHRVTFTGTFVNFVAVPLTTIIVPFGFFTLI
                     SGFLWPALGKLLAGALSLAVAGLISVVHRFALVSHLSYRAPGPSLWMTIIFLMTLASI
                     VMCLRLRFAGRRVVVWSLWGVLAATALIIATYPFSPRVADGKLELTVLDVGQGDSLFV
                     VSPTGKTLLIDGGGAFGGFPGQEQSRGSDPGEEAVSPYLWSRGFKKIDVVALTHAHQD
                     HMGGLIAILENFHIERLWIGREINNNAALARLEALARERNIPVEYETRGRHFLLDEVQ
                     GEFLWPEISPVNAAVAAKNNDSLVLRLKYRNRTLLLPGDAEKQAEHAMLEEHSDGLHA
                     DVLKIGHHGSKNSTTQEFLDAVAPAIAIISAGEDNPYGRPSPELLERLEASGARVLRT
                     DRDGAVHIVTDGDRLEISCFVGCPESPTVTTSRRAQAPNQDQNNQ"
     gene            complement(1446..2003)
                     /locus_tag="VII37_02790"
     CDS             complement(1446..2003)
                     /locus_tag="VII37_02790"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY5068678.1"
                     /translation="MNSAGAFFVRWRVRLGYPLAIAVLFFARPSPRSILYGALVGLVG
                     LVLRAWAAGYLHKQEVLTVTGPYAYTRNPLYLGSAVLALGVAIATRSWISALLLGVYF
                     TVFYSVVMRREEDELRPRHITNFDRYAAAVPLFFPRLSPARLSDSTSAAFSLTQYKKN
                     HEWQAAVGFLLLLIVLVLIWRLRAS"
     gene            complement(2000..3046)
                     /locus_tag="VII37_02795"
     CDS             complement(2000..3046)
                     /locus_tag="VII37_02795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY5068679.1"
                     /translation="MAERRFLVVRLGSLGDIVHTFPAVAALRESFPASEIIWLTHPRY
                     ELLVQCTGLASKIWTIDTRSPSSLMKTIREIRAASFDTAIDYQGLWKSASLPFLAGVK
                     RRIGFSPESVREFGVPLLYTDRVRATIAHVADQNGELSKRAGAHNPVANFQLNPPPMQ
                     PEEVSKYLPSSGMKKFVVLSPGGGWRSKCWPPERFGALCQKIFQSLALRSLINYGPGE
                     EDLASKIGAASGGADPVFFNGELGPLMSLLRDAICVIGGDTGPLHLAVALGTPVVALF
                     GPTDPARNGPYRNPVSSSSVQSSNRQDALDAVLRAPHVVTTHSRQDHTHPSMLEIQVE
                     TVFDAVRRIVEARK"
     gene            complement(3039..3506)
                     /locus_tag="VII37_02800"
     CDS             complement(3039..3506)
                     /locus_tag="VII37_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY5068680.1"
                     /translation="MSQQKLKSLVADHKKRGQKVVFANGGFDILHVGHVRYLEAARQH
                     GDVLIVAVNSDASLRTLKGPARPILDEAARARLVAALRAVDYVVIFSEPTVASLLRDL
                     LPDVHAKGTDYSPETVPERAIAQSLDIQIAIVGDPKNHSTSSLLSSLRKAPHG"
     gene            complement(3534..>3797)
                     /locus_tag="VII37_02805"
     CDS             complement(3534..>3797)
                     /locus_tag="VII37_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5068681.1"
                     /translation="HGMALFESGKRLTHIPVHGSDQSVDVTGAGDTVLATFTLALACG
                     ASPLESAHLANIAGALVVMKRGTATVTRAELLAAIHDQAGVSA"
BASE COUNT          812 a          885 c         1270 g          830 t
ORIGIN      
        1 cgacaaagga tgttgcgtat gagccgcggc caacgcagtt tcgcatcgat cttcgattgc
       61 tggcacgtgc gatggaaatt cggttgcctg ggcgaattgc gagagtgccg gggagtgcgt
      121 tagttggcgg gatggcgttc agctttcggg tatgggaatt gtttgtgctg acactggctt
      181 tgcagatcgg gatgttgccg gtgctggcga gcgcatttca tcgcgttact tttacgggga
      241 cgtttgtaaa tttcgtggcg gtgccgttga cgaccattat tgtgcctttt gggtttttta
      301 cattgatcag cggattttta tggccggcgc taggcaagtt gctggcaggg gcgttgtcgt
      361 tggccgtagc gggactgatt tctgtggtgc ataggttcgc gcttgtatcg cacttgagtt
      421 accgcgcacc cggcccgtct ctttggatga cgattatttt tctgatgacg ttggcgagca
      481 tcgtcatgtg tttgcgcctc cggttcgcgg ggcgcagggt ggtggtgtgg agtttgtggg
      541 gcgtattggc tgcgaccgcg ctgatcattg ctacttatcc attttctccg cgagtggcgg
      601 acgggaagct tgaacttacg gtgctggatg ttgggcaagg agattccttg ttcgtggttt
      661 caccgacggg aaagactttg ttgattgatg gtggcggcgc ttttggggga ttccctgggc
      721 aagaacaatc acgaggaagc gatcctggcg aggaggctgt gtctccctat ctgtggtcgc
      781 gggggttcaa gaaaatcgat gtagtggctt taacgcacgc gcaccaggac catatgggag
      841 gattgatcgc aattctggaa aattttcaca ttgaacgact gtggattggg cgcgaaataa
      901 acaataatgc ggcgctggca agactggagg cgctggcgag ggaacgaaac atacctgtgg
      961 agtacgaaac gcgcggaaga cattttttgc tcgatgaagt gcagggggag ttcttgtggc
     1021 cggaaatttc gccagtcaac gcagccgtgg cggcaaagaa taatgattcg ttggtattgc
     1081 gtttgaaata ccggaacaga acgttactgc tccccggtga cgcggagaaa caggcggagc
     1141 atgcgatgtt ggaggagcat tccgacggtc tgcatgcaga tgtattgaag attgggcatc
     1201 atgggagcaa gaattcgacg acgcaggaat ttctggacgc ggtagcaccg gcaatcgcga
     1261 tcatttcggc tggggaagac aatccttatg gacgtcccag cccagaattg ttggaacgat
     1321 tggaagcaag tggggcgcgg gtgctgagaa cggaccgtga tggcgccgtg catattgtca
     1381 cggacggcga ccgattagag atttcgtgct tcgtggggtg cccggaatca cctactgtga
     1441 ctacctcaag acgcgcgcaa gcgccaaatc aggaccagaa caatcagtaa caataaaaag
     1501 ccgacggctg cttgccattc gtgatttttc ttatattgag tgagcgagaa cgccgcgctg
     1561 gtgctgtctg agagtctagc gggagacaat cttggaaaga agagcgggac tgcggcggcg
     1621 tagcgatcaa aattggtgat gtgacgaggg cgtagctcgt cctcttctct tcgcatcacg
     1681 actgaataaa agacggtgaa atacacgccg agtaaaaggg cggagatcca cgagcgtgtt
     1741 gcgatggcca cgcccaaggc gagcacggcg cttccgaggt agagcggatt gcgagtgtag
     1801 gcgtaggggc cggtgacggt gagtacctcc tgtttgtgga gatagcctgc ggcccaggcg
     1861 cgcaggacca ggccgaccag gccgactaat gctccgtaca aaatgctgcg agggctcggc
     1921 cgcgcgaaaa ataagactgc gattgccagg ggatagccga ggcgaactct ccatcgcacg
     1981 aaaaatgcgc ctgccgaatt catttgcggg cctcgacgat acgacgtacg gcgtcgaaca
     2041 cggtttcaac ttgaatttca agcatggatg gatgtgtgtg gtcctggcgg ctgtgagtgg
     2101 tgaccacgtg tggagcgcgg agaactgcat cgagcgcatc ctggcgattc gaagattgaa
     2161 cggatgaaga gctgaccgga ttgcgatacg gcccatttcg cgcgggatcc gtggggccaa
     2221 acaaggctac tactggcgtg cccaacgcga ctgccaagtg aagggggccg gtgtcgccgc
     2281 ctataacaca aatggcatcg cgcaaaagtg acattagcgg gccgagttcg ccgttgaaga
     2341 acacggggtc ggcgccaccg cttgcggcgc cgattttgga ggctaggtct tcctcgccgg
     2401 ggccgtaatt tatcagagag cgaagcgcca gcgattggaa tattttttgg catagcgcgc
     2461 caaagcgttc aggaggccaa cacttggaac gccagccgcc tccggggctt aagacgacaa
     2521 actttttcat accggagctg ggcaaatact tggaaacttc ttcgggttgc atgggcggcg
     2581 gattgagttg gaaatttgcg actgggttgt gtgctccggc gcgcttagac aattctccgt
     2641 tttggtcggc gacgtgtgcg atggtggcgc ggacgcgatc ggtgtaaagg agcggcacgc
     2701 caaattctct aacagattcc ggggaaaaac cgatgcggcg ttttacacca gcgaggaaag
     2761 gcaggctggc ggatttccaa aggccctgat agtcgatggc ggtgtcgaat gaggcggcgc
     2821 gaatttcgcg aatcgttttc atgagggaag agggtgagcg tgtgtcgatg gtccatattt
     2881 tggaagccag accggtgcat tgcaccagga gttcgtaacg cgggtgagtg agccagataa
     2941 tttctgaagc aggaaaagat tcgcgcaagg cggctactgc cggaaatgta tgcacgatat
     3001 cgccgagaga cccgaggcgc acgaccagga agcggcgctc agccatgcgg cgccttgcgg
     3061 agtgaggaca ataacgagct ggtggagtga tttttgggat ccccgacaat ggcgatttga
     3121 atatcgaggg actgagcgat ggcgcgctcg ggcacggtct cgggagagta gtcggttcct
     3181 ttggcgtgaa cgtctgggag caaatcccgc agtagtgatg caacagtggg ctcgctgaag
     3241 atcaccacat aatcgacggc gcgaagtgcg gccacgagcc gagcgcgagc ggcttcgtcg
     3301 aggatggggc gtgctgggcc tttcagggtg cgcaggctgg cgtcgctgtt tacggcaaca
     3361 atcaagacgt cgccgtgctg acgcgcggct tccaggtagc ggacatgacc gacgtgcaag
     3421 atatcgaaac cgccgttcgc gaacaccacc ttctgaccgc gctttttgtg gtcggcaacg
     3481 agagacttga gtttttgttg cgacacgatt ttcgattccg atgagcgcat aggctaggcg
     3541 gaaactccgg cctggtcatg gatggcggcc agaagttcgg cgcgcgtcac tgtggcggtt
     3601 ccgcgcttca tgactaccag ggcgccagcg atatttgcga ggtgcgcgga ctccaggggc
     3661 gaggcgccgc acgcgagggc tagcgtaaag gttgcgagaa cggtgtcgcc tgcgccggtt
     3721 acatcgactg attgatcgct gccatggacg ggaatatggg tgaggcgttt accggattcg
     3781 aataaggcca tgccgtg
//
LOCUS       DATCBB010000250         1179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_429752, whole genome shotgun sequence.
ACCESSION   DATCBB010000250 DATCBB010000000
VERSION     DATCBB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429752"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VII37_02810"
     CDS             <1..185
                     /locus_tag="VII37_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068682.1"
                     /translation="GPQGPALFKKLAAQGILVRERTREIGPGLVRISIGTASEMKRVV
                     KIIEQYLNRNSARGRE"
     gene            186..776
                     /gene="hisB"
                     /locus_tag="VII37_02815"
     CDS             186..776
                     /gene="hisB"
                     /locus_tag="VII37_02815"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY5068683.1"
                     /translation="MPRKATIHRKTNETNIRLSLNLDGHAKARISTGIRFFDHMLELV
                     ARHGGFDLQITARGDLDVDQHHTVEDVGIAIGEAVLKAVGNRRGILRAGYFVMPMDES
                     LAVAAIDLGGRPFCVVDAKIAAARVGDFQTELVEDFFQGFAQAARANVHLRVLYGRSS
                     HHQVEALFKAFARALRFAVARDKRLRGVLPSTKGLL"
     gene            773..>1179
                     /gene="hisH"
                     /locus_tag="VII37_02820"
     CDS             773..>1179
                     /gene="hisH"
                     /locus_tag="VII37_02820"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY5068684.1"
                     /translation="MKISLIDYGAGNLPSVERTLHRLAFPTERVDSPEGVANASALIL
                     PGVGHYAALIRALDKNGLRDALIDAIARGVPFLGICLGLQALYQMSEEAPDLQGLKIF
                     PGRVRDLTGNVKLPHMGWNRVQARNGSRLLDGI"
BASE COUNT          245 a          314 c          372 g          248 t
ORIGIN      
        1 ttggtccgca aggtccagcg cttttcaaga aactggctgc ccagggaata ctagtgcgcg
       61 agcgaaccag agagattggc cccggccttg tacgcatcag catcggcacc gcatccgaaa
      121 tgaagcgggt ggtcaagatc attgaacaat acttgaatcg aaattcagcg cgagggagag
      181 aataaatgcc tcgcaaagct accattcacc gcaagaccaa cgaaaccaac atcaggctta
      241 gtctgaatct ggacggccac gccaaagcgc gcatctccac cggaatcaga tttttcgatc
      301 acatgctgga gctggtggcg cgtcacggcg gattcgatct gcaaatcact gcgcgcggcg
      361 acctcgacgt ggatcagcat cacactgtcg aagatgtggg catcgccatc ggcgaggcgg
      421 ttctgaaagc ggtgggaaat agacgcggga ttctgcgcgc cggctatttt gtgatgccca
      481 tggatgaatc cctggcggtc gccgcaatcg atctgggcgg gcgtcccttc tgcgtggtcg
      541 acgccaagat tgccgcggcg cgcgtcggcg attttcagac cgaactggta gaggattttt
      601 ttcaggggtt tgcgcaggcg gcgcgggcca acgttcattt gcgcgttttg tatgggcgtt
      661 cttcgcatca ccaggtggag gcgctgttca aagctttcgc gcgggccttg cgctttgccg
      721 tggcgcgcga taagcgcctt cgcggtgtgc tgcccagcac caaaggtttg ttgtgaaaat
      781 ttcgctcatc gattacggcg cgggaaatct tccttccgtg gaacggactt tgcatcggct
      841 cgcgtttcct accgaaagag tcgactcgcc ggaaggagtg gccaatgcat cggcgctgat
      901 actgccgggg gtcggccact atgccgcgct gatccgtgcg ctggacaaaa acgggctgcg
      961 agacgcgctg attgatgcca tcgcgcgcgg cgtacctttc ctgggaattt gcctggggct
     1021 gcaggcgctg tatcaaatga gcgaagaagc tcccgacttg caggggctga aaatttttcc
     1081 aggccgggtg cgcgatctaa cgggaaacgt gaagttgccg cacatgggtt ggaatcgagt
     1141 gcaagccagg aacggatcgc ggttgctgga tgggatcga
//
LOCUS       DATCBB010000251         1614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_40795, whole genome shotgun sequence.
ACCESSION   DATCBB010000251 DATCBB010000000
VERSION     DATCBB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40795"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VII37_02825"
     CDS             <1..1069
                     /locus_tag="VII37_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5068685.1"
                     /translation="FVYFTSTEKSPMERHLYRVGLDGTAFTRITKEPGTHAVNFAPSA
                     GSPPGSNVGTASASVSGFIDTYSNTMIPPRQDLLRADGSRVVVLNENKVAALVSYYLS
                     PVEFLQVKTHDGVMLNAWMIKPPNFSPQRKYPVLVYTYGGPHVQVVMNEWARSTFLWH
                     QMMAQEGYIIFALDNRGSAGRGHLFEEPIHYRLGAQELSDQRDGAAYLKSLAYVDANR
                     MGIWGWSYGGHMTLHAVFEDWNDFKAGFAGGPVTDWHYYDSIYTERYIGLLPQNEEYY
                     KASSPIDKVKQFRGKLLIAHGTGDDNVHFANTLALINDLIENGKYVEVLPFPGRGHGV
                     ADAPARRLLMERVTRFFLDNL"
     gene            1251..>1614
                     /locus_tag="VII37_02830"
     CDS             1251..>1614
                     /locus_tag="VII37_02830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5068686.1"
                     /translation="MHSGKKHSSSNAGWGNPPWQIDFSSAARALPSEVDFAIVGGGFS
                     GLSAAARLRQLQPDRTTAVFEADCVGAGSSGHTGGMTLAESAAGDLPGLGDVLEGFAE
                     VQRELKIKCDFNSPGVWEL"
BASE COUNT          355 a          399 c          465 g          395 t
ORIGIN      
        1 atttgtttat ttcacgtcca ctgaaaaatc tcccatggag cgccatcttt atcgcgtagg
       61 tctcgacggc actgcattta cgcgcatcac gaaagagccg ggcactcatg ccgtgaactt
      121 tgcgcccagc gcgggtagcc cgccgggcag caatgtgggg actgcaagcg catctgtaag
      181 tggatttatc gatacgtatt cgaacaccat gatccctccg cgtcaggatt tattgcgagc
      241 tgatggttcg cgtgtcgtgg tgcttaacga aaataaagtc gcggccctcg taagctatta
      301 cctttctccc gtcgaatttc tgcaagttaa gacgcatgac ggcgtgatgc tcaacgcctg
      361 gatgatcaag cccccgaatt ttagtccgca aagaaaatat cccgtgttgg tttataccta
      421 cggcggtccg cacgtgcagg tggtgatgaa tgaatgggcc cggtcgactt ttctgtggca
      481 tcagatgatg gctcaagagg gttacattat ttttgcgctt gataatcgcg gctccgcggg
      541 acgcggtcat ctttttgagg agcccataca ttaccggctt ggtgcgcagg agctgtctga
      601 ccagcgcgac ggcgcggcgt atttgaagtc gctagcgtat gtcgacgcca atcgcatggg
      661 catttgggga tggagttacg gcgggcacat gacgctgcac gccgtgtttg aagattggaa
      721 cgatttcaag gccggttttg cgggcggccc tgttactgac tggcattact acgattcaat
      781 ttatacggaa cgctacatcg gactgttgcc gcagaacgaa gagtattaca aggcctcttc
      841 tcccatcgac aaggtgaaac aatttcgcgg gaaattgctg attgctcatg gcacgggtga
      901 tgacaacgtg catttcgcga atacactcgc gctcatcaat gacctgatag aaaacggaaa
      961 atatgtggaa gtactgccgt ttcctggccg cggccacgga gtcgctgacg cgcctgcgag
     1021 gcgcctgctg atggagcgag taacgcgctt ctttctggat aatttgtgac cataacttcc
     1081 gctattggca tggacgaaac tacgtaacga gtctggcatc agcaacctaa cgcgaaagac
     1141 gcaaaccgcc ttccgagctt tctgcatcca acgttagtca cctaatctgg ccgtgtattg
     1201 caatttgtgt gtggcaagtt tgtactctgc gaaagacaag gggatttgcg ttgcattcgg
     1261 gcaaaaaaca ttcatccagt aatgccggtt ggggcaatcc accgtggcaa atcgatttta
     1321 gctctgcggc tcgcgctttg ccgtctgaag tggattttgc cattgtgggc ggaggcttca
     1381 gtgggttgtc ggcagcggcg agactaaggc aacttcagcc tgacagaaca actgcggtat
     1441 ttgaggccga ttgcgtcggt gcgggctcca gcgggcacac cggaggaatg acgcttgcgg
     1501 agtccgcggc gggcgatttg ccgggcctcg gcgatgttct tgagggattt gcggaggtcc
     1561 agcgcgaatt aaagatcaaa tgcgatttta attcccccgg ggtatgggaa ctcg
//
LOCUS       DATCBB010000252         1419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_8056, whole genome shotgun sequence.
ACCESSION   DATCBB010000252 DATCBB010000000
VERSION     DATCBB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8056"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VII37_02835"
     CDS             complement(<1..529)
                     /locus_tag="VII37_02835"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068687.1"
                     /translation="MLRYFLQRFLMTLPALWLVLTLVFLMIHIVPGDPVEQMLGEGAA
                     PGELAQLRHSLGLDVSLPAQYGHYLSRLAHGDLGNSLKFQAPVRRIIFERYPATLQLA
                     FLALLVCATIAVPAGVLAAHRRGAGADRAVGVLTLFGLAIPNFALGPLLILLFSIELG
                     FLPVSGRGGPAYYILP"
     gene            complement(564..>1419)
                     /locus_tag="VII37_02840"
     CDS             complement(564..>1419)
                     /locus_tag="VII37_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY5068688.1"
                     /translation="ELQIQIQQARERGLLAVGEEKFIVSAIELTQVQVREIMVPRPDM
                     HTLPVESSLDEVMRAFATTQRSRIPVYRGSEDHILGFVHIKDMLWVLLDRERRTAENL
                     PPPAFDLRRVLREVLIVPETKPASELLPELRARHVGMAMVVDEFGSILGLVTLEDILE
                     QVVGEIHDEFDVVEQPLTLADGAVVFDAALNVRDLDTQYNISLPEDPAYATVGGFVLA
                     QLGFIPKGGETFEYGGWRFTVTEMDGKRVARVKIQRIRPIEPAKAGDAPAVTARPQKE
                     TRIAHSDS"
BASE COUNT          357 a          436 c          344 g          282 t
ORIGIN      
        1 cgggaagaat gtagtaagcc ggtccacccc gcccagacac cggcagaaat cccagctcga
       61 tggaaaacag caaaatcagc aaaggcccca gcgcgaaatt gggaatcgcc aacccaaata
      121 gcgtcaacac acccacagca cggtccgccc ccgcgccgcg acgatgcgcc gccagcacac
      181 cagcgggcac cgcaatggtc gcgcaaacaa gcagtgccag gaacgccagc tgcaacgtcg
      241 caggataacg ttcaaaaata atccgccgca ccggtgcttg aaatttcaat gagtttccca
      301 gatcgccgtg cgcaagtcgc gaaagataat gcccgtattg cgcgggcagc gacacatcca
      361 gtcccaaaga atggcgtagt tgcgcgagct ccccgggagc cgcgccttcc ccgagcatct
      421 gctccacagg gtcgcccggc acaatatgaa tcatcaggaa caccagcgtc agaactagcc
      481 agagcgccgg cagggtcatt aaaaatcgtt ggagaaaata gcgcagcatt ttttgaaatt
      541 aataatccag tgctggtgcg aactcaggaa tcactatgag caattcgtgt ttccttttgt
      601 ggacgagccg tcaccgccgg ggcatcgcca gccttggccg gttcaattgg acggatacgc
      661 tgaatcttaa cccgcgccac tcgcttgcca tccatttccg taacggtaaa acgccaccca
      721 ccgtattcga aagtttcccc gcctttagga ataaatccca attgcgcgag aacgaatcct
      781 cctaccgtgg cataagccgg gtcttccggc agggaaatgt tgtattgcgt gtctaaatcg
      841 cgaacgttta acgccgcatc aaaaactacc gctccatccg ctagcgtcaa gggctgctcg
      901 accacgtcaa attcgtcgtg aatctcgccc acaacctgct cgagaatgtc ctccagcgta
      961 actaatccaa gaatgcttcc gaattcgtct accaccatgg ccattccgac gtggcgcgcg
     1021 cgaagttccg gcagcagttc gctggcaggt tttgtctccg gcacgattaa cacttctcgc
     1081 agtacgcgcc gcaaatcgaa tgcgggtgga ggtaaatttt ccgcggtacg acgctcgcga
     1141 tcgagcagca cccacaacat atctttaatg tgcacaaatc ccagaatgtg atcttctgat
     1201 cctcgataaa caggaattct tgagcgttgc gtagtagcga acgcgcgcat tacttcatca
     1261 agcgaagact ccaccggaag agtgtgcatg tccggccgcg gcaccataat ttcccgcact
     1321 tgtacctgcg tcaactcgat ggcgctgaca ataaattttt cctcacctac agccagcagt
     1381 ccccgctccc gcgcttgctg aatctggatt tgcagctct
//
LOCUS       DATCBB010000253         1027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_40866, whole genome shotgun sequence.
ACCESSION   DATCBB010000253 DATCBB010000000
VERSION     DATCBB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40866"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VII37_02845"
     CDS             complement(<1..300)
                     /locus_tag="VII37_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068689.1"
                     /translation="MPVFTFSGKTATGEKVQGERVASTKDGLASQLRKERITPGSIRE
                     KGKEFALPTFGSGKVKVKEVAIFFRQFSVMIDAGLPLVQCLEILGANQENPTFQKT"
     gene            complement(389..>1027)
                     /locus_tag="VII37_02850"
     CDS             complement(389..>1027)
                     /locus_tag="VII37_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5068690.1"
                     /translation="IEFVHMNKNCLVNQRELHSDTKSFTDALRAALREDPDVVLIGEM
                     RDLETIESALRIAETGHLTFGTLHTNSASSTINRIIDVFPAHQQSQIRAQLSLVLEGI
                     MCQSLVPKADNKGRAMCMEILVPNAAVRNLIREDKIHQIYSAMQSGQEKYGMQTFNQS
                     LATAYFQKQITLEVALTRSSNQDELQEMISRGATLTSRSPAPLAKGAVPGKR"
BASE COUNT          184 a          286 c          295 g          262 t
ORIGIN      
        1 tgttttctgg aaagttggat tttcctggtt cgcgcccagg atctccaggc attgcaccag
       61 cggcaagcca gcatcaatca tgaccgagaa ctggcggaag aatatcgcca cttccttcac
      121 tttgaccttg ccgctgccga aggtgggtag ggcaaattcc ttgcccttct cacggatgga
      181 cccgggcgtg atgcgttcct tccgcaactg ggaggcgagt ccgtccttgg tggaggctac
      241 gcgctctccc tgaacttttt cgcctgtcgc tgtctttccc gaaaacgtga aaaccggcat
      301 gattgagttc tcctttatct cgtgtcgcta tctgtgttct gttgcggccc ggcgtgctgc
      361 cagacatttt tctcgtgatc tctcgcagtc atcgttttcc aggcaccgcg cccttggcca
      421 gcggagccgg gctgcggctc gtcaatgtcg cgccgcgcga gatcatttcc tgcaattcgt
      481 cctgattgct ggagcgcgtc aacgcgacct ccaacgtgat ttgtttctgg aaatacgcgg
      541 tggcaagcga ctggttgaaa gtctgcatgc cgtacttttc ctggccggac tgcatcgcgg
      601 aatagatctg gtgaattttg tcttcgcgga tcaggttgcg aaccgccgca ttcggcacca
      661 gaatctccat gcacatggcc cgccctttgt tatcggcctt cggcacaagg ctttggcaca
      721 tgatgccttc gagcaccagc gaaagctggg cgcgaatctg cgattgctgg tgcgctggaa
      781 acacgtcgat gatgcggtta atcgtggaag acgcggagtt agtatgcaac gtgccgaagg
      841 tgaggtggcc ggtttccgca atgcgcagcg cggactcgat cgtttcgaga tcgcgcattt
      901 cgccgatcag cacgacgtct ggatcttcac gaagcgcggc gcgtagggcg tcggtaaagc
      961 tctttgtgtc cgagtgtaat tcgcgctggt tcaccaggca attcttgttc atgtgcacga
     1021 attcgat
//
LOCUS       DATCBB010000254         1088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_202779, whole genome shotgun sequence.
ACCESSION   DATCBB010000254 DATCBB010000000
VERSION     DATCBB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202779"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VII37_02855"
     CDS             <1..572
                     /locus_tag="VII37_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5068691.1"
                     /translation="PVGKGTGLGLSICYGIVTEHGGTIRVRNQPHRGASFTIELPFRI
                     ASENPSEEPQPEVSQTQTRVLCIDSDDAALKLLEQILNAHGHQVHIAKSGQEARAVLD
                     KFEIDVIVADVDFEGPVSQGKLPDWLAGYGSAISQRLIRMHSLDAADVHTGADIPADL
                     VSLKKPLDPALLLNALNTILSARVHSVVR"
     gene            complement(543..>1088)
                     /locus_tag="VII37_02860"
     CDS             complement(543..>1088)
                     /locus_tag="VII37_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068692.1"
                     /translation="VGIGVALLAVLAWLSFPGTPRDSQADLGQRPERETRHISYLPQI
                     KQALGQADYEYLSVRGPAGLSNRVRKERKHIALNYLVALRSDFERLQHIARVVAVMSP
                     EIAVAQELHGMRLNLEFSVRYRIIYFRLLADIAPASAINNLSNLISALTVRMETAISS
                     LGEGAALAAELGSPHNGMNPG"
BASE COUNT          223 a          329 c          311 g          225 t
ORIGIN      
        1 agccggtggg caagggtact gggctgggtt tgagcatctg ttacggcatc gtcactgagc
       61 acggtggcac catccgggtt cgcaaccagc ctcatcgtgg cgcgtctttc accatcgagt
      121 tgccgtttcg aatcgcgtcc gagaatccca gcgaagaacc gcagcccgag gtctctcaaa
      181 cacagacgcg ggttctttgt atcgattccg acgatgcggc gttaaaatta ctcgagcaga
      241 ttctgaacgc gcatggacat caggtccaca tcgcgaaaag tggccaggaa gcgcgtgcgg
      301 tcctcgacaa gttcgagatc gacgttatcg tcgcggacgt ggatttcgag ggacctgttt
      361 cacagggcaa gctgccagac tggcttgccg gttacggttc cgcgatttcg cagcgcctca
      421 ttcggatgca ttcgctggac gccgcggatg tccacaccgg cgcggacatt cccgccgacc
      481 tggtatcgct gaagaaaccg cttgacccgg cgttgctcct gaacgccttg aatacgattt
      541 tgtcagcccg ggttcattcc gttgtgaggt gaccccaact ccgcggcaag cgcggcgcct
      601 tcgcccaagc tgcttatggc tgtttccatt cgcaccgtca gcgcgctgat caggttactc
      661 aaattgttaa tcgcgctcgc aggcgcaata tccgccagca acctgaaata gataatccgg
      721 tatcggacgg aaaattccag attcaggcgc atgccatgta gttcctgtgc cacggcaatc
      781 tcgggagaca tgaccgcgac aacgcgcgcg atgtgctgca gcctctcgaa atcgctccgt
      841 aacgctacaa ggtagttgag cgcgatgtgc ttgcgctctt tccgcacacg attggacagg
      901 cccgcgggcc cacgaacgct cagatattca taatccgcct ggcccaacgc ctgcttgatc
      961 tgtggcaggt aagaaatgtg ccgtgtttcg cgttcgggcc gctggcccag atcagcctga
     1021 gaatcccggg gcgttcccgg gaacgagagc cacgcaagca ccgccagaag cgctacgccg
     1081 atgccaac
//
LOCUS       DATCBB010000255         1545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_332479, whole genome shotgun sequence.
ACCESSION   DATCBB010000255 DATCBB010000000
VERSION     DATCBB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332479"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..505)
                     /locus_tag="VII37_02865"
     CDS             complement(362..505)
                     /locus_tag="VII37_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068693.1"
                     /translation="MERFVHNEYLIHLRKQLAGTADEAKRRQTSTLLAEEEIKDHLPP
                     KEK"
     gene            624..854
                     /locus_tag="VII37_02870"
     CDS             624..854
                     /locus_tag="VII37_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068694.1"
                     /translation="MLDSVAVKAQPKNNRKTNAKRFGLSIRRISMSKAQKGNKENKKP
                     KADKNQSEANVSAYKAAQGQGKPAGSPFAKKT"
BASE COUNT          359 a          423 c          429 g          334 t
ORIGIN      
        1 aatggcgccg cggatgagca gctcgccgcg taccggtaac ggtgcgcccg gcgcgccggc
       61 ggtgcgcgcg gcctgttgcg ccaggagccc ccaaggcagg agcgccgccg ttcccagcgc
      121 agccgctccc tgcatgagcc gacgccggct gaacgtgttg tccatcattt gcattcctcc
      181 aatgtgtcaa cgcatcgcgg gccaaaatcc acgaatgtcc gctgttattc caatagcgga
      241 caaacgcgga catgccgcga atgacgctaa gtgccacaag cagacatccg tccaacgatt
      301 gcatcccagt ataaaattga gagaagccgc caactgatgg gcctgctttt ctctgatggg
      361 cctacttttc tttgggcggt agatggtctt tgatttcttc ttcagccagc agcgtcgacg
      421 tttgccgccg cttcgcctca tctgccgttc ccgctaattg cttccgcagg tggatcaaat
      481 attcgttgtg gacgaagcgc tccataggag cctcccgtag ggcgcatagc cctagggttg
      541 gaagcctgcg ccttttgtac ggtgcggtcc aattaaatgc gacgatctag ttagggctgc
      601 atccaaaaca agaagccgcc attttgctcg actctgtcgc ggtaaaggcg cagcctaaaa
      661 acaaccgaaa aaccaatgca aagcgtttcg ggctttcaat caggagaatc tccatgtcga
      721 aggctcaaaa aggcaataag gaaaataaga aaccgaaggc ggacaaaaac cagtctgagg
      781 ccaatgtctc ggcctacaag gcggcgcaag ggcagggcaa gccggccggc agcccgttcg
      841 cgaaaaagac ctgacgctag ggtcgctcaa gacattaatc ggcggcttgc gaacgcggaa
      901 cgtgattgtc tagctaggcc accttggtgt gtggtgtagt gtggttatgg ggcactgtga
      961 gtgattgctc acgaccgcag tttgcacaga cgaatatctc gcactccaaa tcaaatcctt
     1021 tgtcgccggg ctcgatcagc ttgagccgta gttcaccacc gcactgctcg caatgcgacg
     1081 agggcggcgg ttggatgagg gtgcgcatga gatttccttt cgcccgactc gtagcgaggg
     1141 agcggggcac tcaaaagggc cccgaatgaa gtttggattg aaagcccaaa cgacttgcgt
     1201 taacgttttg agcatataaa gcgacatctt agtttcttca ctcgcgcatt gatccacatc
     1261 aacgccattg aaaattgtag aaactgatcg ccctattctt tggtgttgct cgtcctcaac
     1321 gtatatggcc agagggtgct cacgggcatt taatcatcag cccgctgaaa gtccgctttg
     1381 ggtcgatgct gttgaaaaag gcctcgcgcc cagcggacta gcggcctaag ccgggatcag
     1441 ccactatcaa agtatccgtt ggagccatta caatgcttct tcgggaaggt gaaaggcgga
     1501 agctcgatcg cataagcaat cgcgcctatt gagacgcttc atgtt
//
LOCUS       DATCBB010000256         1761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_332488, whole genome shotgun sequence.
ACCESSION   DATCBB010000256 DATCBB010000000
VERSION     DATCBB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332488"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..914)
                     /locus_tag="VII37_02875"
     CDS             complement(6..914)
                     /locus_tag="VII37_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068695.1"
                     /translation="MSSQYCRFVERGTARRRAPLLEQLLARADDWAPVADWRADAFRV
                     IAPQAACMPAVAAAALCADRGVVDAAWVCVATPVHYVAEMTSVRLPADGMLSLTPAGA
                     ETLASDFNRVWQGSGIRMTAARSAQLFCIFDRAFEVLTRDPEDVLDRHIEEFLPIGED
                     SARLRQLISETEMWLFEHEANRVRTELGLPAINGLWFWGGGAPIASLPTVQGWAAGDD
                     LFFSAFSVPRNEEFQCGVVVAGCVPGSDQWHEVESRWLHPAMIKLRARKIQRLQVSAA
                     DRCFTLTVRGLRRFWRRRKPWWESFE"
     gene            950..1318
                     /locus_tag="VII37_02880"
     CDS             950..1318
                     /locus_tag="VII37_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY5068696.1"
                     /translation="MRFAQDSTSGINIIRAYGNGEIRVNENVYRGALILSATTVIVDA
                     KLQNLDDLIALGLARTLALEPELVLLGTGARQIFPAASFGAQFLRAGIGFEVMDTSAA
                     CRTFNVLVGEQRRVAAMLLV"
     gene            complement(1562..>1761)
                     /locus_tag="VII37_02885"
     CDS             complement(1562..>1761)
                     /locus_tag="VII37_02885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5068697.1"
                     /translation="IKHGENLLSAYAHNRDSLVREGQEVSRGEVIGHMGEGAPNKPVL
                     YFEIRRNGKPIDPLSFLPQVK"
BASE COUNT          408 a          531 c          466 g          356 t
ORIGIN      
        1 tatcgctact cgaatgactc ccaccagggc tttcttctgc gccaaaatcg gcgcagcccg
       61 cgtaccgtca gcgtgaaaca ccgatccgct gcagagacct gcagccgctg tattttgcga
      121 gcccgcagct taatcatagc cggatgcagc cagcgcgatt caacctcatg ccactgatcg
      181 ctgccgggaa cgcagcctgc gaccacaacg ccgcactgaa attcctcgtt ccgaggaacg
      241 ctaaaagcgc tgaaaaataa atcatctccc gcggcccacc cctggacagt cggtagagaa
      301 gcgatcggcg cgccgccgcc ccagaaccaa agcccgttaa tggccggcag acccaattca
      361 gtgcgaacgc gattcgcttc atgttcgaac aaccacattt cagtctcact gatcaattgc
      421 cgcagccgcg ccgagtcctc gccgatcggg agaaattctt cgatgtgccg gtccaatacg
      481 tcctcgggat cgcgtgtcag gacctcgaaa gcgcggtcga aaatacaaaa aagctgcgcc
      541 gatcgggccg ccgtcatgcg aattccagag ccttgccaaa cccggttgaa atccgacgcc
      601 agagtttcag cgccagccgg cgtcagcgat agcataccgt ccgcgggcag gcgcacactg
      661 gtcatctccg ccacgtaatg aacgggggtt gccacgcaaa cccacgccgc atccacgacg
      721 ccgcgatcgg cgcaaagcgc ggcggcggcc acggcgggca tgcaggccgc ttgcggcgcg
      781 atcacgcgga atgcgtcggc gcgccagtcg gctaccggcg cccaatcgtc cgcgcgcgca
      841 agcaattgct ccaggagcgg agcgcggcgg cgcgcggtgc cgcgctcgac aaaccgacaa
      901 tactggctag acacaaacgc tccggcagca atgtgagtat cttaagcgca tgcgattcgc
      961 ccaggattca acttcaggca tcaatatcat ccgcgcctat ggaaatggcg agattcgtgt
     1021 gaatgagaac gtttatcgtg gtgcgctcat cctgagcgcg acaacggtga tcgtcgatgc
     1081 gaagctccaa aatctcgacg atctgatcgc cttaggactc gcgcgcacgc ttgccctcga
     1141 accggaactg gtgctgctcg gcaccggcgc gcgccagatc tttcccgctg catccttcgg
     1201 cgcacagttt ttacgtgccg gcattggatt tgaggtcatg gataccagtg cggcgtgccg
     1261 cacgttcaac gtcttggtgg gggaacagcg tcgtgtcgca gcgatgctgc tggtgtgaga
     1321 tttaagaaaa aatagcaggg cgataagcaa aatacacaac caaaccatta tcgagaaata
     1381 ttcttcttct aattgatctt gttgttgttt tttgattaaa cgtgtaagcc tagcgccccg
     1441 caatggatct tcccgacggc tcgcagtaaa acatacgaaa ttaactgtcg caagtccccg
     1501 acaagtttag taaatactct atatttactt atttcatgta gttacgtaac taaaatagat
     1561 attacttcac ttgtgggagg aaacttagcg gatcgattgg tttgccgttg cggcgaattt
     1621 cgaagtacaa cacaggttta ttgggcgctc cctcgcccat gtgtccaatc acctcgcccc
     1681 gcgatacctc ctgaccctca cgcaccagcg aatcgcgatt gtgcgcatag gcggaaagca
     1741 agttctcgcc atgcttgatg a
//
LOCUS       DATCBB010000257         3651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_364824, whole genome shotgun sequence.
ACCESSION   DATCBB010000257 DATCBB010000000
VERSION     DATCBB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364824"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VII37_02890"
     CDS             <1..1053
                     /locus_tag="VII37_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068698.1"
                     /translation="PAVVLDADRESASHAASAAKPEIPAESLLQTAPAEPIVEPPSEA
                     HSDAASQTDTETPGKEKGDQLVQNLIDSFNTTPKKPDNKYADAIAAWKLHSSGWNVDN
                     GFFSIWGYWRWVAVGAVAGLAVLLAIGVTAHRLPWSNATPISVSKAGQSLTSAVNPQN
                     QTPGNIHNAAAGAGNAVGNEIRSDQHASDTANNVNGALNSVTTPPTLPPALASALPKP
                     DQAPCINLGPNGGKIRIYLWSEKDTPQAIVGTYAKNLRAVQDVQLVDKAPYDLVLYVN
                     GAQVNASGAEAGFMWSSRVFRPWYCGQSLGLLEQTQVNESLHYVQGANLEQRIQAEVA
                     FLILHTFESIRSEHQK"
     gene            1295..1900
                     /locus_tag="VII37_02895"
     CDS             1295..1900
                     /locus_tag="VII37_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068699.1"
                     /translation="MSPQWTKWSILPATLLVAATVSFAQDPTPEVAQRKENQQDRIAQ
                     GVKSGQLTAGETANLETKEAAINQETRADRAANGGKLTASEKAQVNQQQNQVSKQIYA
                     DKHNANTAHYGNGVVGQRRENQQDRIAQGVKSGQLTAGETAKLENQQRGINQQVRADR
                     AANGGKLTAAEKVQVNHEQNQASKNIYAKKHNARTQGTTKK"
     gene            2098..3462
                     /locus_tag="VII37_02900"
     CDS             2098..3462
                     /locus_tag="VII37_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5068700.1"
                     /translation="MAIASINPATGEVLKTFEPLNAAQIEAKLTLAAKTFETFRKTSF
                     TDRARLMNKAADILENEKESLGKLMTLEMGKTYRSAVDEAVKSAWGCRYYAENAERFM
                     ADEVIETSASRSYVHYQPLGPILVVMPWNYPFWQVFRFIAPGLMAGNVGVLKHASNVP
                     QCALKIEEIMLRAGFPEGAFQTLLIGVKEVDAILNDPRIQAATLTGSEEAGKQVGMSA
                     AKRIKKVVLELGGSDPFIVMPSANLDDAVATAVKARVGNNGQSCIAAKRFIIADEIAD
                     KFERAFVSKMESLKVGDPMDAKTDVGPLATAGAVESLQADVQKSIDAGAKVLTGGKPN
                     GGPGYFYPPTVLTNIPKDSPAGSEELFGPVASLFRVKNAQDAVRVANDHRYGLGASAW
                     TNDDRERDLFIKELEAGMVFINQMVASDPRVPFGGVKRSGHGRELSINGIREFTNIKT
                     VWIK"
BASE COUNT          930 a         1015 c          986 g          720 t
ORIGIN      
        1 ccggcagttg tgttagacgc agatcgagaa agcgcctcgc acgctgcgtc cgcggctaag
       61 ccagaaattc ctgcggagtc gttactccaa actgctccag ccgagcccat cgtcgaaccg
      121 ccaagcgaag cgcactctga cgcagcgagc caaacggata ctgaaacgcc cggcaaagaa
      181 aaaggcgacc agttagtcca gaatttgatc gactccttta acaccacgcc aaaaaaaccg
      241 gacaacaaat acgctgacgc aattgctgca tggaagctgc acagctcagg ctggaatgtg
      301 gacaacggtt ttttttctat ttggggatat tggcggtggg ttgccgtggg ggccgtggcc
      361 ggtctggcgg tgttgctcgc gattggcgtc accgctcatc gcctgccctg gagcaacgcc
      421 acacccatca gcgtttcgaa agccgggcag tcgctaactt cggcagtgaa cccgcaaaat
      481 cagaccccgg ggaatatcca caatgccgcc gccggcgcgg gcaacgctgt tggcaatgag
      541 attcgcagtg atcagcacgc cagcgatact gcgaacaatg ttaacggagc gcttaattcc
      601 gtcaccaccc cgccaaccct tcctcccgcg ctggcttctg cgttgcccaa gccggaccag
      661 gctccttgca tcaacctcgg ccctaacggc ggcaaaatcc gcatttatct ctggtcggaa
      721 aaagacactc ctcaagctat cgtgggaact tacgcgaaga atttaagagc ggtgcaagat
      781 gtccagctgg tggacaaagc cccatatgac ctggtgctgt acgtcaacgg cgcgcaagtc
      841 aacgcgagtg gtgccgaagc cggatttatg tggagctccc gcgtatttcg tccctggtat
      901 tgcggccaat cgctgggcct gctcgagcaa acgcaggtca acgaaagttt gcattatgtg
      961 cagggcgcaa acctggagca acgcattcaa gccgaagtgg ccttcttgat cctgcacacc
     1021 tttgaaagca tccgcagcga acatcagaaa taagctgcga gcgggacgct tcactccgcc
     1081 agtgttttcg ttattattgc cgccaattct tctcttcttc ttcgccaatg cagcctgaaa
     1141 tgtgcccaaa aagatacacg aattcgcagg gcatgtagca aaacctgtcc ttccgggcaa
     1201 ttgtttgaca gcaactccgc atcgagaatg gtgtttgagg aagtagcccc gcggagtttt
     1261 acgcaaaacg gaaagtaaaa aaggagagaa tttcatgagc cctcaatgga ccaagtggag
     1321 tattttgccc gctaccctgc tggtggcggc aacggtttcc tttgcgcagg accccacgcc
     1381 ggaagtcgcg cagcgcaagg aaaatcagca ggaccgcatc gcgcaaggcg tgaagagtgg
     1441 gcaacttacc gctggtgaaa ccgctaatct cgaaactaaa gaggcggcca tcaaccagga
     1501 aacccgagct gaccgcgcgg ccaacggcgg aaaattgact gcctcggaaa aagcccaggt
     1561 taaccagcag caaaatcagg tgtcgaagca aatctatgcc gacaaacaca atgccaacac
     1621 cgctcattat ggcaacggcg ttgtggggca gcgccgcgaa aatcagcagg atcgcattgc
     1681 gcagggcgta aagagcggtc agttaaccgc cggcgaaacc gctaagctgg aaaatcagca
     1741 gcgcggaatc aaccagcaag tacgcgccga tcgcgcagcc aacggcggca agttgaccgc
     1801 tgctgaaaaa gtgcaagtca accacgaaca aaaccaagcg tcaaaaaaca tttacgccaa
     1861 gaaacacaat gcccggacgc aaggcaccac aaagaaataa gtcatctgca atttataaag
     1921 gaaaaggcac tcttcagtga gtgccttttt tcttttgccc acctttcatc gccgctcttg
     1981 gtaagtcttc tcgaccgcca acatttttct cgcagaacac atataattta taggtacgac
     2041 cacaacgaag ctaaacagcc gctcactatc gatgaagcct ctggaggctt agtaaatatg
     2101 gcaatcgcgt ctattaatcc cgcaacggga gaagtcttaa aaacatttga gccgctcaac
     2161 gctgctcaga tcgaagcgaa attaaccctg gcggctaaga ccttcgagac atttcgcaag
     2221 accagcttta ccgatcgcgc gcgcctgatg aataaggcgg ctgacattct ggaaaacgaa
     2281 aaggaatcgc tcggcaagtt gatgaccctg gaaatgggca agacctaccg ttccgccgtc
     2341 gatgaagccg tgaaatccgc gtggggttgc cgctattacg ccgaaaacgc ggaacgcttc
     2401 atggccgatg aagtcatcga gaccagcgcc agccgcagct acgtgcatta ccagccgctc
     2461 ggtcccattt tggtggtgat gccctggaat tatcctttct ggcaggtttt tcgttttatc
     2521 gcgccgggtt tgatggccgg aaatgttggc gtgctcaagc atgcttccaa cgttccgcaa
     2581 tgcgcgctga aaattgaaga aattatgttg cgcgccggat tcccggaggg cgcgtttcaa
     2641 acgctgctga tcggcgtcaa agaagtggac gcaattttaa acgatccgcg catccaggcc
     2701 gccacgctga ccggcagcga agaagccggc aagcaagtcg gcatgtccgc ggccaagcgc
     2761 atcaaaaaag ttgtgctgga attgggcggc agcgatcctt tcatcgtgat gccgagtgca
     2821 aatctcgacg atgcggtggc taccgccgta aaagcccgcg tcggaaacaa cggccaatcc
     2881 tgtatcgccg cgaaacgttt catcatcgcc gatgaaattg ccgataagtt tgagcgcgcc
     2941 ttcgtcagca agatggaatc gttgaaggtt ggcgatccga tggacgccaa gaccgacgtt
     3001 ggcccgctgg ccaccgccgg tgccgtggag tcgctgcaag ccgatgtgca aaaatccatc
     3061 gacgcaggcg caaaggtttt aaccggcggg aaaccaaacg gaggaccggg ctatttttat
     3121 ccgccgacgg tgctcaccaa cattcccaaa gattcgccgg ccggcagcga ggaactgttt
     3181 gggccggttg cttctctatt ccgcgtgaaa aacgctcaag acgcagtgcg tgtcgccaac
     3241 gatcaccgtt acgggttggg agcgagtgcc tggaccaacg atgatcgcga gcgcgatttg
     3301 ttcatcaagg agttggaagc cggaatggtt tttatcaacc agatggtggc gtccgatccg
     3361 cgcgtgccct tcggcggagt gaaacgctca ggccacggcc gcgaattgag catcaacggc
     3421 attcgcgaat tcacgaatat aaaaacggtg tggatcaaat agggacgccg ctttaggaag
     3481 acttcttagg aaggcttctt aggaagacgt cgcttcatga aagcgtttct ccagccgccg
     3541 ccaatcgctg cgcggcgctc tccggcgtaa tatcccgttg cggcgaaccc agcaattcat
     3601 aaccgaccat aaatttttgt actgttgcgg accgcaataa cggcgggaag t
//
LOCUS       DATCBB010000258         1528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_364879, whole genome shotgun sequence.
ACCESSION   DATCBB010000258 DATCBB010000000
VERSION     DATCBB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364879"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VII37_02905"
     CDS             complement(<1..815)
                     /locus_tag="VII37_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068701.1"
                     /translation="MATTDRFSRLEFQRMLDVTEKQLNYWEKLRLVAPRKSGVEKSYD
                     FRDLITLRTAKQLIEKGVPAQRLQRSIVALQQKLAEVQSPLTELRIRSNGRDVIVERA
                     GVHLEPISGQIMLNFETRELTEKVRMMPQRSATDWVVLGVKREEQSASQTGPRLAAEA
                     YRSALETDQANVDALNNLGTLSYEEGNLEDAIALFRHAVKLHPDHMLSHFNLGSALEE
                     TGHDDQARRHLRRAVHLDPEYADAQYNLAFVCDKLGAYVEARRHWQKYLELDP"
     gene            complement(826..>1528)
                     /locus_tag="VII37_02910"
     CDS             complement(826..>1528)
                     /locus_tag="VII37_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY5068702.1"
                     /translation="KVERSEIVKGYEYEKDQYVLFSDDDLDKIEPDSARAMEILEFVK
                     VDEIDPLYYDASYYLTPEDEGKKAYSLLLKAMEDSGYAAIAKVTMHQREHIVVIRARE
                     QGMTLHTMFYQSEIREVAEFTHQDKVEPKEQEKKLAMQLIESLAATFEPEKYRDEYTE
                     SMRSLIAAKQKGQEVTATTHPVRAPVIDLMDALRKSINAKPAASASKKPPVRSISSAS
                     QATQSSRKSSKKAVG"
BASE COUNT          294 a          419 c          407 g          408 t
ORIGIN      
        1 tcaggatcga gctccaaata tttttgccag tggcggcgag cttccacata cgcgccgagc
       61 ttgtcgcaca caaaggccag attgtactgt gcgtcggcgt attccggatc gagatgtacg
      121 gcgcggcgca aatgccggcg agcctggtcg tcgtgtcccg tttcttccag cgcgctccca
      181 agattgaaat gagacagcat atggtccgga tgtagtttca cggcgtgtct gaaaagagcg
      241 atcgcatctt ccagattgcc ctcttcgtag gaaagcgtgc ccaaattatt cagcgcatcc
      301 acgttggcct gatcggtctc cagcgcggac cgataggctt ccgctgccaa acgggggccg
      361 gtctgtgatg cactttgttc ttcgcgtttc acgcccaaga ctacccagtc ggtcgcgctg
      421 cgctgcggca tcatgcgaac tttttccgtt aactcgcgag tctcaaaatt gagcatgatc
      481 tgtccggaga ttggttcgag gtgcacgcca gcgcgctcca cgatgacgtc ccggccgttc
      541 gaacgaatgc gcaattcagt tagcggagac tgcacttcgg cgagtttctg ttgcaacgcc
      601 acgatggagc gctgcagccg ctgcgctggg accccttttt ctattagctg cttggccgtg
      661 cggagggtaa tcagatcgcg gaagtcgtaa gatttctcga ctccgctttt tcggggtgct
      721 accaggcgca gtttttccca gtaatttaac tgcttttcag tcacatcgag catgcgttga
      781 aattcgaggc gcgaaaatcg atcggttgtt gccatcaaat aaaaactagc ctaccgcctt
      841 tttgctcgac ttgcgactgg attgcgttgc ctgtgacgcc gaagatatag agcgcaccgg
      901 gggttttttg ctggcgctgg cagccggctt tgcgttgatg ctcttgcgca atgcatccat
      961 caagtcaatt accggagcgc gcaccgggtg agtcgtggcc gtaacttcct ggcccttttg
     1021 tttggcggca atcaacgaac gcatgctttc cgtgtactcg tcacgatatt tttccggttc
     1081 aaaagtcgct gccaggcttt cgattaattg catggccagc ttcttttcct gttccttggg
     1141 ttccacttta tcctgatggg tgaattccgc aacttcgcgt atttcgctct gataaaacat
     1201 ggtgtgcagc gtcatgccct gctcgcgcgc gcgaatcacc acgatgtgtt cgcgttggtg
     1261 catggtcact ttggcgatgg ccgcgtagcc ggaatcttcc atggccttca gcaaaagcga
     1321 ataagctttt ttgccttcgt cttccggagt caaataatag gaggcgtcgt aatacaacgg
     1381 atcgatttca tccactttta cgaattcgag gatttccatc gcgcgcgcgg agtccggctc
     1441 aatcttgtcc agatcgtcat cgctgaacag cacgtactga tccttctcgt attcgtagcc
     1501 cttgacgatc tcggagcgct ctactttt
//
LOCUS       DATCBB010000259         4500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_267918, whole genome shotgun sequence.
ACCESSION   DATCBB010000259 DATCBB010000000
VERSION     DATCBB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267918"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..211)
                     /locus_tag="VII37_02915"
     CDS             complement(26..211)
                     /locus_tag="VII37_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068703.1"
                     /translation="MMAILHFTTLVITTLFAAGAAALVNWLLLRATFQLMRPATAGRA
                     STGPAAGTLLSRVRVSS"
     gene            447..1595
                     /locus_tag="VII37_02920"
     CDS             447..1595
                     /locus_tag="VII37_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5068704.1"
                     /translation="MPTKSGFRIGSILGIPIYLHASWLLIFGLITYSLGMQFTQSHPQ
                     WSSTQHWTVGVLTSMLFFGSVLFHELSHSVVAMHYKIRVASITLFLFGGVARIGREPS
                     KAIQEFNIAIAGPLSSYFLCAFFFGLTLLFPFSDALGALGLWLAQINFAVATFNLLPG
                     FPLDGGRIFRAAVWGATKNFTRATRVASVSGQLVAYGLILVGAWFAAIGHDWQSGLWL
                     ACIGFYLQSAARDSIAQLAIRETLAGLRASDVMNNEVPIINRNTSLEEYSAEVLRTGR
                     RCHLVVTEDRLVGMMNVQALNSVPRDEWNSNSVQAVMIPRERISWAAPEEPLLRLLER
                     LLAADINQMPVVSVSGEDDAQVIGMVTRDSILRVMQTRAELGAAIAAK"
     gene            complement(1621..3012)
                     /locus_tag="VII37_02925"
     CDS             complement(1621..3012)
                     /locus_tag="VII37_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5068705.1"
                     /translation="MKDKLSSKFPPGPEPGALDGIFSPFRRDPLSFLTAAARQYGDIV
                     GLRFLHYRTYLISHPDHIEDVLVNHPRKFVKGRVLQANKRLFGNGLLTSEGDFWLRQR
                     RLAQPAFHRTRIASYGETMVRYTEQMLERWKHGDEIDAHQEMMQLTLNIVGKTLFDAD
                     VAGDAREIGSTITMLLNFSSDFRRLIFTPKWLPTPRNIQTTLAIRRLNKIVFRMIADR
                     RASGRDAGDLLSMLLAARDEDGSQMDDKQLRDETLTLFLAGYETTANLLAWTWWLLAT
                     HPEVERKLHQELDGLLGGPSPSMDDLPRLPYAGHVLTESLRLYPPAWGMARLAIEDHE
                     IAGYPVPAGTGVAIAQWVVQRDPRWYEAPDEFRPERWEGDLQKRLPRFAYFPFGGGPR
                     QCIGNVFALMEATLVLAMVAQHFRLRMMPNQEILPLASITLRPKNGVAMRVESRRASK
                     SDLNIPAAVLNGI"
     gene            complement(3044..3628)
                     /locus_tag="VII37_02930"
     CDS             complement(3044..3628)
                     /locus_tag="VII37_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5068706.1"
                     /translation="MNSLENWFCASRLWQNLTERRWLPWALEGSDLGEHVLEVGAGPG
                     AATADLARRARSVTSLEYSHDFCIRLQSKIDATKVVQGDASVLPFKSQTFTSAIAVLV
                     LHHLRSSDAQDRAFAEIFRVLKPGGVFVALEIQDGWFTRVLHFRSTFVPVSQDELNKQ
                     LRDAGFSNISVINRSGGIRIRAERIADVNAKVPR"
     gene            complement(3662..>4500)
                     /locus_tag="VII37_02935"
     CDS             complement(3662..>4500)
                     /locus_tag="VII37_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068707.1"
                     /translation="GSQNKFVDHEMIAAGHPDLFFELDSFSAVEPRHWKTPRESASGK
                     PVEDAAWSGVRDWSTGQAVQLSASLERLSWRAKSERYDKKDVWPEYSELSCFGCHHAL
                     VPAKDSWRQAHEFPGRRPGDPAWNESRYAVFRLIAKQVDPTAAQELDKHLATLANTMS
                     TLNPDRAAVSSEASVAAPIAQRMAERLAAMPYDQDLALRMLQQITSDAENISLADERA
                     AEQATMAIDSLYIAYSKEAKPKNAEEVRDAINGLFQQLENPSSYNADQFAAALRRLHG
                     ML"
BASE COUNT          972 a         1269 c         1238 g         1021 t
ORIGIN      
        1 cgaacgtgtt cgttcgacgc caggattagc tagaaacgcg cacccgactg agcaaagtcc
       61 ctgccgccgg gccagtcgat gctctgcccg cggtggctgg gcgcataagt tgaaatgtcg
      121 ctcgtaacaa cagccaattg accagcgccg ccgcaccggc ggcaaatagg gtggtgatta
      181 ccagcgtcgt gaagtgcagt atggccatca tggtggctcc tctttacgtc ggcgtgatga
      241 atcgcacgct gcacaccaca gcaaaggcaa aggaaccaaa cgagttattc agggggcagc
      301 acgcagcttt gtgggggcac agacacgctg aggccgcatt gactcagctg ccctgtgttt
      361 ggcggcgcgc tgaagaaagg cgtaatctag atatagcaac ttaggaaatt gcctgagcgg
      421 cggctcgctt gggctagtca cggatcatgc caacaaaatc aggctttcga atcggtagca
      481 tcctcgggat ccccatctac ctgcacgcca gctggctgct gattttcggg ttgataacct
      541 attccctggg tatgcagttc acacagtccc acccgcagtg gagttccacg cagcattgga
      601 cggttggtgt cctgacaagc atgttgtttt tcggttcagt tctctttcat gagctctcgc
      661 atagcgtcgt cgccatgcat tacaaaatac gcgtagcttc catcacgttg tttcttttcg
      721 gcggagttgc gcgcattggc cgcgagccgt cgaaagccat ccaggaattc aatatcgcca
      781 tagctggtcc gctctcgagt tactttctct gtgctttctt ttttggttta actctgttgt
      841 ttccttttag cgacgcgctt ggggccttag ggctatggct ggcgcagatt aattttgccg
      901 ttgccacgtt taatttattg cctgggtttc ctctcgacgg aggcagaatc tttcgcgcgg
      961 ccgtttgggg cgcgacgaaa aattttactc gggccacacg cgtcgccagc gtaagtggcc
     1021 aattggtcgc ttacggactg attttagtcg gcgcctggtt cgccgccatc gggcacgatt
     1081 ggcagtcggg actgtggtta gcttgtattg ggttttattt gcagagcgcg gcgcgcgata
     1141 gcattgcgca attggccata cgcgaaacgt tggcgggctt gcgcgcttca gacgtgatga
     1201 acaatgaagt tcccatcatc aatcgcaata cttctctcga agagtacagc gcagaagttc
     1261 taaggaccgg gcgtcggtgc catctggtgg tgaccgaaga tcgcctggtg ggcatgatga
     1321 acgtgcaggc cttgaactcg gtgccgcgag atgagtggaa cagcaattcc gtgcaagcgg
     1381 tcatgattcc gcgcgaacgc atttcctggg cggcacctga agagccgctg ctgcgcttgc
     1441 tcgaacgatt gctcgccgcc gacattaatc aaatgccggt agtcagcgta tcgggggaag
     1501 acgacgcgca ggtgattggc atggttacgc gcgactccat cctccgcgtt atgcagacta
     1561 gagctgagtt gggagccgct atagccgcca agtgacgctt cccagcggcc aaggcgttct
     1621 tcaaatgccg ttaaggaccg ccgccggaat atttagatcg gacttggacg ctcgcctcga
     1681 ttccactcgc atcgcaaccc cgttcttggg tcgtagcgta atcgaggcca gcggcagaat
     1741 ttcttgattc ggcatcatcc tcaatcgaaa gtgttgcgca accatcgcga gcaccagcgt
     1801 agcttccatt aacgcgaaca cattgccgat acactggcgc ggaccgccgc caaacggaaa
     1861 atacgcaaag cgcggcagcc gcttctgcaa atccccttcc cagcgttcag ggcgaaattc
     1921 gtctggcgct tcgtaccaac gcggatcgcg ttgcaccacc cactgcgcga tcgcgactcc
     1981 agttcctgcg ggcactggat accctgcgat ttcatgatct tcaatcgcca gccgcgccat
     2041 gccccacgcc ggcggataga gccgcagcga ctccgtcaaa acgtgtcccg catacggcaa
     2101 tcgaggcaaa tcatccatcg atggagaagg cccgccaagg agtccgtcaa gttcctggtg
     2161 taattttcgt tctacctctg gatgggtcgc aagcagccac catgtccaag ccaacaggtt
     2221 ggcagtggtt tcatagcccg cgagaaacag cgtcagcgtt tcgtcgcgta attgtttgtc
     2281 gtccatctga gaaccgtctt cgtctcgcgc agccagcagc atagataaaa gatcgcccgc
     2341 atcgcgaccc gaagcgcggc ggtccgcaat catgcgaaag acaattttgt tcaaccggcg
     2401 aatggccaga gtggtctgga tgttacgcgg ggtaggaagc cacttgggag taaagatgag
     2461 gcgccgaaaa tcggaactga aattcagcag catcgtaatg gtgctgccaa tttcgcgagc
     2521 atcgcctgct acgtcagcgt caaataaagt tttgcccacg atatttaacg tgagttgcat
     2581 catctcctga tgggcgtcga tttcgtcgcc atgcttccag cgctccaaca tttgctcggt
     2641 gtagcgcacc atggtctcgc cgtaagaagc gatgcgcgtg cgatgaaacg ctggctgcgc
     2701 cagccgacgc tgccgtagcc agaaatcgcc ttcgctggtc agcaacccat ttccaaatag
     2761 acgtttgttg gcctgtagta cacggccttt cacgaatttt cgcggatgat tgaccagcac
     2821 atcttcgatg tggtcaggat gagaaatcag gtaagtgcgg taatgcagaa accgcaaccc
     2881 gacaatatcc ccatactgtc gcgctgcggc ggtcagaaag cttagaggat cgcgccggaa
     2941 cgggctgaag atgccatcga gcgcgcccgg ttcgggccca ggaggaaatt tggaggaaag
     3001 cttgtccttc atcttcggat gttaagcgca gccggtgacg acgtcaacgc ggcaccttcg
     3061 cgttaacatc agcaatgcgt tctgcgcgta ttcggattcc gccactgcga ttgatgacgc
     3121 tgatgttaga aaatcctgcg tcgcgcagct gcttattcag ctcatcttgc gagacgggca
     3181 cgaaggtgct gcggaagtgc aagacgcgcg taaaccatcc atcctgaatt tcaagcgcga
     3241 caaaaacacc acccggtttt agcaccctaa aaatttctgc aaatgctcgg tcttgcgcgt
     3301 cactcgagcg caggtgatgt aacaccagta ccgcaatggc tgaagtaaat gtctgcgatt
     3361 tgaacggcaa caccgaagca tcgccttgga ccacctttgt ggcatcaatc ttagattgca
     3421 acctgatgca gaaatcatgg ctatattcca ggctggtaac gcttcgcgcg cgccgcgcca
     3481 agtccgcggt ggcggcccct gggccagcgc cgacttccag aacatgttct ccaagatccg
     3541 aaccctcaag cgcccatgga agccacctgc gttctgtaag gttctgccac aatcgcgaag
     3601 cacagaacca attttcaagg ctattcatgc tgtgggtgac cgcttagacg acgaaacagg
     3661 gttacaacat tccatgaagt cgccgcaggg ccgcggcaaa ctggtcggca ttgtaggaag
     3721 atgggttttc caactgctga aacaatccat tgattgcatc gcggacttcc tcggcattct
     3781 tgggcttggc ttccttgcta tatgcaatgt acagcgagtc aatggccatg gtggcctgtt
     3841 ccgcggcacg ttcgtccgcc agcgagatat tctcagcatc gctggtaatc tgttgcaaca
     3901 tccgaagtgc caggtcttga tcgtatggca ttgccgccag acgctctgcc atgcgctgcg
     3961 cgatcggcgc agcaaccgaa gcctcggacg atacagccgc gcgatcagga ttgagagtgc
     4021 tcatcgtgtt ggccagcgta gctaaatgtt tgtccagttc ctgcgctgcg gttggatcca
     4081 cctgtttcgc gatcaaacga aacaccgcat atcgagattc gttccacgct ggatcgccag
     4141 ggcgtcgccc cggaaattcg tgcgcttgcc gccaactgtc tttcgccgga acgagcgcgt
     4201 ggtggcagcc gaagcacgag agctctgaat attctggcca tacatctttt ttgtcgtagc
     4261 gctcgctctt ggctctccag ctcaatcttt ctagtgaagc gctcaattgc actgcctgac
     4321 cggtgctcca gtcccgcact ccgctccacg cggcatcttc aacgggttta ccggacgccg
     4381 actcgcgggg cgtcttccag tgacgtggtt ccacagctga aaatgaatcc agctcaaaaa
     4441 aaagatctgg atggcccgcg gcgatcattt cgtggtctac aaacttattt tgcgagccca
//
LOCUS       DATCBB010000260         1127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_8329, whole genome shotgun sequence.
ACCESSION   DATCBB010000260 DATCBB010000000
VERSION     DATCBB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8329"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VII37_02940"
     CDS             complement(<1..479)
                     /locus_tag="VII37_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068708.1"
                     /translation="MKLPRNIPCVRRQAGVLLVECLVYIAVFGILVGGGTAVFYFCWD
                     HSQAVILATDDITAALRAGERWRTDVRAATGNISVLTTPTGELVRIPETEKEVVYRFE
                     SNAVRREIPALHHSWLLLPKVKTSQMTADSRGAVSAWRWELELQERRKETHLPLRFT"
     gene            complement(476..868)
                     /locus_tag="VII37_02945"
     CDS             complement(476..868)
                     /locus_tag="VII37_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068709.1"
                     /translation="MKMLPAIDYFHASNRMRGYLMVDLLVALAILSLAIMPIGFAFAR
                     ERQVLKLEYCQSVANEIVDGEMEILAAGDWKNYPDGVQNYPIHANAAASLPPGHFQLN
                     KTGNHLRLEWNSDAKHGLGTIAREVTIQ"
     gene            complement(865..>1127)
                     /locus_tag="VII37_02950"
     CDS             complement(865..>1127)
                     /locus_tag="VII37_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068710.1"
                     /translation="DDAVRVFDDAGEFHWRLTNATHAHGGFLNALLGWHEALDAKAFQ
                     QEETATHAMTSGLVILNGLVVALIATAMFGILIAILKGALLQ"
BASE COUNT          255 a          327 c          317 g          228 t
ORIGIN      
        1 aaggtgaacc gaagcggcag atgcgtttcc ttccggcgct cctgcaattc cagctcccag
       61 cgccacgcgc tcacggcgcc acgcgaatcc gccgtcattt gcgaggtctt gactttcggc
      121 agcagcagcc aggaatgatg caaggcaggg atttcgcggc gcacggcatt cgattcaaag
      181 cgataaacaa cttccttttc tgtttcaggg atccgcacca gttcgccggt tggggtagtt
      241 aggacggaaa tgttcccggt cgcggcgcgc acgtccgtgc gccagcgttc gcccgcgcgc
      301 agggctgccg taatgtcgtc ggttgcgaga atcaccgcct gcgaatgatc ccagcaaaaa
      361 taaaacacag cggtgccgcc gccgaccagg atgccgaaca cggcgatgta gacgagacat
      421 tccaccagca ggacccctgc ctggcggcgg acacacggaa tatttcgcgg cagtttcatt
      481 gaatggtgac ttcgcgggca atcgtgccaa gaccatgttt cgcatcggag ttccattcga
      541 ggcgcagatg attgccggtt ttgttcagtt gaaaatgtcc cggcggcagg ctcgcggcgg
      601 cgttggcgtg aatgggataa ttttgaactc cgtccggata gtttttccaa tcaccggcgg
      661 cgagaatttc catttcgccg tccacgatct cattggccac gctctggcaa tattccagct
      721 tgagaacctg cctttcgcgg gcaaaagcaa agccaatcgg catgatggcc agcgagagaa
      781 tcgcgagggc cacgagcaag tccaccatca aatagccgcg catgcgattc gatgcgtgaa
      841 aataatctat ggccggaagc attttcattg cagcaatgct cccttcaaaa ttgcgatgag
      901 aatgccaaac attgccgtgg caatgagcgc gacgacgagg ccattcagga tcaccaaacc
      961 gctggtcatg gcgtgcgtcg cagtttcctc ctgctgaaac gccttcgcat cgagagcttc
     1021 gtgccagccg agcaacgcat tcaaaaaacc gccgtgcgcg tgcgtggcgt ttgtgagccg
     1081 ccagtgaaat tcgccagcgt catcaaacac gcgaacagca tcatcga
//
LOCUS       DATCBB010000261         1053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_300394, whole genome shotgun sequence.
ACCESSION   DATCBB010000261 DATCBB010000000
VERSION     DATCBB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300394"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VII37_02955"
     CDS             complement(<1..182)
                     /locus_tag="VII37_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5068711.1"
                     /translation="MGFWETRRVVVTGGHGFLGSFVVDRLRAAGAKEVVVPRSEKHDL
                     RIQTEALRLYTDTRPD"
     gene            complement(241..>1053)
                     /locus_tag="VII37_02960"
     CDS             complement(241..>1053)
                     /locus_tag="VII37_02960"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY5068712.1"
                     /translation="VSFDVPEYTGDITGLGTMRLLEAVRRSGCKTRFYQASSSEMFGS
                     APAPQSENTVFQPRSPYAAAKVYAYWMTKIYREGYGMHAANGILFNHESPRRGETFVT
                     RKITRGVANILAGKENTLYLGNLESRRDWGYAPEYVEAMWQVVQQDAADDFVIGTGEA
                     HSVKEFVEEAFSYAGLEWQKHVKISDRYFRPLEVDTLIADTTKSRAALGWEARVKFHD
                     LVAIMVDADVEALGLKSPGRGKLILEEKIGSWNRWHNSVTRLSQAVQGQASH"
BASE COUNT          233 a          316 c          264 g          240 t
ORIGIN      
        1 atgtcgggac gagtgtcggt ataaagccgg agcgcttcgg tttggatccg tagatcgtgc
       61 ttctcgctgc gcggaaccac tacttctttg gcgcccgcgg ccctcagccg gtccaccacg
      121 aaggaaccaa gaaagccgtg gccacctgta accacaacac gacgagtttc ccaaaagccc
      181 atgtgccctc aatcgccagt gatttgtatc ccgtgatttt aattgatggg aacggaattt
      241 ctagtgtgac gcctgccctt gcaccgcttg agacaaccta gtcacggaat tgtgccagcg
      301 attccatgac ccgatctttt cttccagaat aagtttgccg cgacccggtg atttcaagcc
      361 caaagcttcg acatccgcat ccaccatgat ggccaccaga tcatggaatt taacacgcgc
      421 ctcccaaccc aatgcagcgc gggattttgt ggtgtcggcg ataagcgtat ccacttccaa
      481 aggacgaaag tatcgatcgg aaattttcac gtgcttttgc cactctaatc ccgcgtagga
      541 gaaggcctct tccacaaatt ctttcaccga gtgcgcttcg cccgtgccga taacaaagtc
      601 atccgcagca tcctgctgca ccacttgcca catggcttcc acgtattccg gcgcataccc
      661 ccagtcgcgg cgcgactcca gattgccgag atataaggta ttttctttgc cggccagaat
      721 attggcgacg ccgcgcgtaa ttttacgcgt aacgaacgtt tctccccggc gtggactttc
      781 gtgattaaag agaatgccat tggcggcgtg catgccgtaa ccttcgcggt aaattttggt
      841 catccaatag gcgtaaactt tcgccgcagc atagggactg cgcggctgaa acacggtgtt
      901 ctcgctttgc ggagcgggcg ccgacccaaa catctcagag ctggaggcct gatagaaacg
      961 cgtcttgcat ccgctgcgcc gcaccgcctc gagcagacgc atagtgccca gcccggtgat
     1021 gtcacctgtg tattccggca catcgaagct gac
//
LOCUS       DATCBB010000262         1499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_234737, whole genome shotgun sequence.
ACCESSION   DATCBB010000262 DATCBB010000000
VERSION     DATCBB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234737"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VII37_02965"
     CDS             <1..484
                     /locus_tag="VII37_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5068713.1"
                     /translation="ASKLSPVGAEVDDQSMTLLKFADGKIGYVGSCWTSPGVFAVRVF
                     GSKGLMHYEIDFGTWDTPDKLHESSTLYIQRGKNGYGSREELKVPPSDMFRTELELFA
                     ESCRTGKPNELTAENGNAAVAIVNAALRSIERRGQEVTISEAMESARKANTNRAQHVA
                     "
     gene            474..1277
                     /locus_tag="VII37_02970"
     CDS             474..1277
                     /locus_tag="VII37_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY5068714.1"
                     /translation="MLPSRYFVELTQPEIAEQIKKNPLVILPAGSVEQHGPHLPTGTD
                     IFAANVIAHAVAERMDGLVLPGGPLGVTPLHMPFEGTITLSADTFMRVVTETAASTAQ
                     HGAKYLLILNWHELNIPPLGVAAETLHRDFGMTVLTVQACFVAEELFGKECNGLTHGG
                     EIETLAVMAHRPELVHLERIDYSSDHAHGKKIDGLRRTRSYQPVLTDVRSIAPTGWYG
                     SPQHATMEKSTRMLQVVADSIAKDAKEVFRQLDIIQGGTAELKNSKRTE"
     gene            1277..>1499
                     /locus_tag="VII37_02975"
     CDS             1277..>1499
                     /locus_tag="VII37_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068715.1"
                     /translation="MARVFTQSAAKSLGLPGRTALELVSGVGGASGITLRKVEIPVPA
                     LGDSPRSRHVHSDFEECIYVLAGEGTTEAD"
BASE COUNT          344 a          335 c          486 g          334 t
ORIGIN      
        1 agcctcgaaa ttgtcgccgg taggcgccga agtagatgat cagtcgatga ctttgttgaa
       61 attcgcggat ggaaaaattg ggtatgtagg ttcgtgctgg acttcaccgg gagtttttgc
      121 ggtgcgggtg tttgggtcga agggattgat gcattacgag atcgattttg gtacctggga
      181 tacgccggat aaattgcacg agagctcgac gctctacatt cagcgcggga aaaatggata
      241 tggcagccgt gaagagctga aagttccgcc gagcgatatg tttcgcacgg agctggaact
      301 gtttgctgaa agctgccgga ccggtaagcc caatgaactt acggcggaaa atggcaatgc
      361 ggcggtggcg atcgtgaatg cggcgttgcg ctctattgaa cgcagggggc aagaggtgac
      421 aatttcggaa gcgatggaat cggcacgcaa agcgaataca aatagggcgc aacatgttgc
      481 ctagccgcta cttcgtggaa ttgacacaac cggaaattgc cgagcaaatt aaaaagaatc
      541 cgctggtgat tttgcctgcc ggcagcgttg aacaacacgg gccgcatctt ccaacgggca
      601 cggatatttt tgctgccaat gtgatcgctc atgccgtggc ggagcgcatg gatgggttgg
      661 ttttgccagg ggggccgctg ggggttacac cgctgcacat gccttttgaa ggcaccatca
      721 cgctgagtgc ggatacgttt atgcgcgtgg tcaccgagac ggccgcgtca accgcgcagc
      781 acggcgcgaa gtatctactg atattgaatt ggcatgagct gaatattccg ccgctgggcg
      841 ttgccgctga gacgctgcat cgtgactttg ggatgacggt tttgacggtg caagcttgtt
      901 ttgtggcgga agagttgttt ggaaaagaat gcaacgggct cacgcatggc ggcgaaattg
      961 aaacgctggc agtgatggcg caccggccag aattggtgca tttggagcgc atcgattatt
     1021 catcggatca tgcacatgga aaaaagatcg atggcttgcg tcgcacgcgc agttatcagc
     1081 cggtgctcac ggatgtgcgg tccatagcgc cgacgggatg gtacgggagc ccgcagcacg
     1141 ccaccatgga aaagagtacg cgcatgttgc aagtggtggc tgattcgatc gccaaagatg
     1201 ctaaggaagt tttccggcaa ctggacatca ttcaaggcgg tactgccgag ttgaagaatt
     1261 cgaaaaggac ggagtaatgg cgcgagtttt cactcagagc gccgcgaagt cgcttggcct
     1321 gccggggcgc acggcgctag agcttgtttc tggcgtgggt ggcgcttctg gaatcacgct
     1381 gcgcaaggtg gagattcctg ttcccgcttt gggcgattca ccgagaagcc gccatgtgca
     1441 tagcgatttt gaagaatgta tttatgtgct tgcgggtgaa ggtacgacag aagcggaca
//
LOCUS       DATCBB010000263         1911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_41179, whole genome shotgun sequence.
ACCESSION   DATCBB010000263 DATCBB010000000
VERSION     DATCBB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41179"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..1531)
                     /gene="ftsW"
                     /locus_tag="VII37_02980"
     CDS             complement(440..1531)
                     /gene="ftsW"
                     /locus_tag="VII37_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY5068716.1"
                     /translation="MAKRVSVDGWMFTVTLLLVFIGLVMVFSASAVMAKERYGSAYFF
                     LLRQMGWAVVGIVAMVIAMKVDYRRYKHPAVVFSLIGVTSLMLISVFFLDRAHNTHRW
                     IHFGGFSFQPSEIAKPAIILFLAFFLESRTKSMGDWRNTLLPAIVPTLFFLGLIVFEP
                     DLGTAIACAAITASVLFVAGLDMRYLGYALAASILPLYFLIFHVAYRRARILAFLDPY
                     SDPQGRGFHIIQSLIAVSTGGITGVGLMEGKQKLFYLPEPHTDFIFAVTAEELGLVGA
                     LIIVALFSIFLWRGIRTALRTQDMFGRFLAAGITSMVVLQAFINISVVLGLMPTKGIP
                     LPFISYGGSSLFVTLACVGVLLNVTKQAE"
BASE COUNT          507 a          542 c          492 g          370 t
ORIGIN      
        1 aaaagcactt ccgcggcgta attattttta agttcttgcg cgatggcgag cccggggatt
       61 acgtgccccc cggttcctcc gcccgcgata attgcccgca tgcatagact ataaatgccg
      121 gtcccgcaaa tcgcgctctt cgggcgcgtg ctctcgcctc acttaacccc ccggacttcc
      181 gtccagggct aagattagcc gctccttcgg agctttagcc cgcgcacaat tcaatgatag
      241 atttgcacat cagcaaattg cgttcttgta aagttgacag ttcgcgggcg cagacaccag
      301 ccccagcggg gcgaaccagc ttagccccgg acgcaagtcc ggggaaagcg accctgctcg
      361 aacacgcctt tgcgaagcaa aggcaactcc attcacacct cttcagcagt ggaaattgga
      421 aacgatctca accgctccat cactccgctt gcttcgtaac gttcagcaat actcccacac
      481 acgccaacgt gacgaacaac gacgatcctc cgtaggagat gaagggaagt ggaatacctt
      541 tggtcggcat cagtccgagc acaacgctga tgttgataaa tgcttgcagc accaccatac
      601 tggtgattcc ggcggcaaga aaacgtccga acatatcctg ggtgcgcagg gccgtgcgaa
      661 taccgcgcca caaaaatatc gagaagaggg caacgataat aagcgctccc accaggccaa
      721 gctcttccgc ggtaacggca aagatgaaat ccgtgtgcgg ctccggcaga taaaacaatt
      781 tctgttttcc ttccatcaat ccgacgccgg taattcctcc ggtactcacc gcgattagcg
      841 actggatgat gtgaaagccg cgtccctgcg gatcggaata aggatcaaga aacgccagga
      901 tgcgggcgcg ccgataggcc acgtggaaaa tcaggaagta gagcggcaag atcgaagcag
      961 ccagcgcata tccgagataa cgcatatcga ggccggccac gaacagaacc gatgctgtga
     1021 tggcagcaca cgcgatggcc gttcccagat caggctcaaa aacaatcagt ccgagaaaaa
     1081 acaaagtagg aacgattgcc ggcaaaagcg tatttcgcca gtcccccatg gacttggtgc
     1141 ggctctccaa aaagaatgcc agaaaaagaa tgatggcagg cttggctatt tcggaaggct
     1201 gaaaagaaaa tccgccaaaa tgaatccagc gatgcgtatt gtgtgcgcgg tcgagaaaga
     1261 acaccgaaat caacatcagt gaagtgacgc cgatcaatga aaataccacc gccggatgct
     1321 tatagcggcg atagtcgacc ttcatcgcga tgaccatggc aacgatgccg acgacagccc
     1381 atcccatttg ccgcaacaaa aagaagtaag cggacccata gcgttctttc gccatcaccg
     1441 ccgacgcgct gaacaccatc accaggccga taaataccag cagcaaagtt accgtaaaca
     1501 tccagccgtc gacactgaca cgctttgcca tgggaggatt tcatttcacc acgcgccgcg
     1561 aaaaaattaa gtttttagat cggcgattgt tgattgtctt ggtacgaggc agaattcacc
     1621 acgaagccac ggatccaccg ggaagcggta tattgcggaa aatttatcga gagaatcgac
     1681 acgcgaagtc gaccgggccg ccttctccgt gcctcggtgt ctcagtggtg gaagttcggg
     1741 ttacagccct ttcacagtct ctttgaaaac cctgccccgg tgctcataat tctggaactg
     1801 gtcgaagctg gcgcaggctg gcgctaagag gacaatatcg ccaggctggg ccgctctggc
     1861 ggcgcacttc acggctgcat ccaaagtttc ggtgtggacg atttctgtcg c
//
LOCUS       DATCBB010000264         1069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_300495, whole genome shotgun sequence.
ACCESSION   DATCBB010000264 DATCBB010000000
VERSION     DATCBB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300495"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="rlmN"
                     /locus_tag="VII37_02985"
     CDS             complement(<1..925)
                     /gene="rlmN"
                     /locus_tag="VII37_02985"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5068717.1"
                     /translation="MGVTLSQNTELFGKTKEELRELCVSLGEPAYRGEQIYRALYAER
                     KFDLAQVTNLPAALREQLASSAPLSLPEIKKRFQSADGAIRYLFELGASGKDKTRPAS
                     VEAVFMPSEGRQTICASTQAGCAVNCQFCLTAQLGLIRNLTPGEILAQILIALEENKD
                     TLVPHTNIVFMGQGEPLLNDDAVLTACRILQDPNGVGIPAKRVTISTSGIVPGIERLA
                     MESERPKLAISLNASNDAQRDALMPINRKYPLAALLGACRKYPLRSWEFLMFEYVMLG
                     GINDSLEDAKRVVKLLAELKCKVNLIPWNPGD"
BASE COUNT          239 a          343 c          243 g          244 t
ORIGIN      
        1 aatcccctgg attccacgga atcaaattga ccttgcactt cagctcggcg agcaacttca
       61 ccacccgctt cgcgtcctcc agcgagtcgt taatcccgcc aagcatcacg tactcaaaca
      121 tcagaaactc ccagctgcgc aaagggtact ttcgacaagc tcccaacagc gccgccagcg
      181 gatatttgcg gttgatgggc atcagcgcgt ctcgttgcgc atcattggat gcgttcagcg
      241 aaatcgccaa cttgggccgc tcgctttcca tcgccaaccg ctcgattccc ggcacaatcc
      301 cgctcgtcga gatcgttacc cgttttgcag gtatccccac gccattcgga tcctgcagaa
      361 tcctgcacgc cgtcagtacc gcgtcatcat tcagcagtgg ttcgccttgc cccatgaaca
      421 caatattcgt gtgtggcacc agcgtgtctt tgttttcttc gagcgcaatc aggatttgcg
      481 ccaggatttc tcccggcgtc aaattgcgaa tcaatccaag ttgcgccgtc aaacagaatt
      541 ggcaattcac cgcgcagcct gcctgcgtcg aagcgcaaat ggtctgccgc ccttcgctgg
      601 gcatgaacac tgcttccacg gaagcaggcc gggtcttgtc tttcccgctc gcacccaact
      661 caaaaaggta acggatcgcc ccgtccgcgg actggaatcg tttttttatt tccggcaaag
      721 aaagcggtgc cgaacttgcc aactgctctc gcagggccgc cggtaagtta gtcacctgcg
      781 ccaggtcaaa ttttctctcc gcatacaacg cgcgataaat ctgttcgccc cgataagcag
      841 gctcgcccag cgacacgcac agttcgcgca attcttcctt agtcttccca aacaattcag
      901 tgttttgcga caaggtcacg cccattcgac tccagcttca agaatactcc gaacagccct
      961 gatcgtctcg taagccagcg ccaccacgca aaaacggcgt tataattaca actgacaaat
     1021 ttttagcagc attgccttgg agggcttcat ggctggctca acagctgaa
//
LOCUS       DATCBB010000265         1155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_106066, whole genome shotgun sequence.
ACCESSION   DATCBB010000265 DATCBB010000000
VERSION     DATCBB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106066"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VII37_02990"
     CDS             complement(<1..1069)
                     /locus_tag="VII37_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5068718.1"
                     /translation="MSFSRSAIAQLWARSDRSAVRTFLLALAALTVALLLALYSGAAA
                     ELGQLLLASSSAIAALAVAGWVAVTLVPVLARRTPLRWIGYKVEYRVSRAGWVYLGGI
                     ILVALAALNTGNNLLFLVLACLIAIILMSGILSSTTLSGVEMRLELPEHIFAGQPVRA
                     TIELQNEKRTLPSFSLRVEAAKPKGAPAPAMLETPVYFAYLPKGGKVQQTVPMTFSRR
                     GVHRQDTFRIVTRFPFGFLQKARRMDLKTEALVYPSVAPTDELVEILPGLQGALESLS
                     KGRGQDLYALRGYVANDSVRHVHWKASARSGSLMVREFTREDDCRVLLVLDPHISPVL
                     NAGSAPTMQTASERFERAITLC"
BASE COUNT          262 a          393 c          303 g          197 t
ORIGIN      
        1 cgcacagcgt aatcgcgcgt tcgaatcgct cgctggccgt ttgcattgtc ggcgcgcttc
       61 ccgcattcag caccgggctg atgtgcgggt ccagcaccag cagcacccgg caatcgtcct
      121 cgcgcgtgaa ttcccggacc atcagcgagc ccgagcgcgc cgaagctttc cagtgcacat
      181 gccgcacgct gtcgttggcc acgtagccgc gcagcgcata caaatcctgc ccgcgccctt
      241 tcgacaaact ctccagcgcg ccctgcagcc ctggcaatat ttccaccagc tcatcggtcg
      301 gcgccaccga tggatagacc agcgcttccg tcttcaaatc catccgccgc gctttctgca
      361 aaaatccaaa cggaaaccgc gtcacgatgc gaaaagtatc ctggcggtgt acgccgcgcc
      421 gcgaaaacgt catcggcacg gtttgctgca ccttgccacc ctttggcaga tatgcgaaat
      481 acacgggagt ttccagcatg gccggcgcag gcgcgccttt gggcttcgcg gcttccactc
      541 gcagcgaaaa actcggcagc gtgcgcttct cgttttgcaa ttcgatcgtg gcgcgcaccg
      601 gttgccccgc aaaaatatgc tccggcagct ccagtcgcat ctccacgccc gacagcgtcg
      661 tcgaagaaag aattcccgac attaagatga tggcgatcag gcacgccagc accagaaaca
      721 aaagattgtt gccggtattc agcgccgcca gcgccaccag aataattccg cccagataaa
      781 cccagcccgc gcgagaaacg cggtattcca ctttgtagcc gatccagcga agcggcgtgc
      841 gccgcgccag caccggcacc aaagtcaccg ccacccatcc ggctaccgcc agcgccgcga
      901 tcgctgaaga acttgccagc agcaattgtc ccagctccgc cgccgcgccc gaatacagcg
      961 ccagtaggag cgctacggtc aacgccgcca gcgccagcaa aaacgtgcgc accgccgagc
     1021 ggtccgaacg ggcccacagt tgcgcgattg ccgagcggct aaagctcatg gggaggattc
     1081 attccgaagt catttcagtt tcagaaaaaa atatcaggct taaaaaaaga aagtatcggc
     1141 accatcaaaa tgagt
//
LOCUS       DATCBB010000266         1238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_365072, whole genome shotgun sequence.
ACCESSION   DATCBB010000266 DATCBB010000000
VERSION     DATCBB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365072"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VII37_02995"
     CDS             <1..504
                     /locus_tag="VII37_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5068719.1"
                     /translation="RMDAISVSKERYNRMSAPAQRMIIQSIQRAMSPLPSAMKKPLAK
                     TERNLERVEKLVGLFEPFILQNEHDFVADNVEKLSQALTPEEKEIFGYDTAGLDWWEY
                     WINIHIPALRRWTYPLIEGRPLEARAPRLLQMPQRTAAVTTTAATTPENGDAMKTGTN
                     GATWRYS"
     gene            489..>1238
                     /locus_tag="VII37_03000"
     CDS             489..>1238
                     /locus_tag="VII37_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5068720.1"
                     /translation="MAIFLTGGTGYIGAHVAANLLEQHDAALNLLVRGRDARDAELRL
                     WNALQLHLPFPKFYEYLQSKIRIFRGDITLPQFGLDPDSYDRLVHTTDSVLHCAASLN
                     RKSEKNCLNVNLRGTLEVVQLARRSQYYHGLRRFSNVSTVAVAGKRSHEIISEDRSIE
                     WDRSDYDPYARTKKFCEHMVRELLPDVPLTIFRPSIVLGDSRYPQTTQFDMVRSFVFL
                     AELPVLPFRPHDKIDIVNVDFVAAAISTLHMK"
BASE COUNT          275 a          323 c          357 g          283 t
ORIGIN      
        1 cggatggatg cgatttcggt ttctaaggaa cgctataacc gcatgtcggc gccggcgcag
       61 aggatgataa tccagtcgat tcagcgggcg atgtctcctc ttccctccgc aatgaagaag
      121 cctctcgcaa agacggagcg aaacctggaa cgcgtggaaa agttagtagg attgtttgag
      181 ccttttattc tgcaaaacga gcacgatttt gtcgcagata atgtggagaa gttgtcgcag
      241 gcgttgaccc ccgaagagaa agaaattttc ggctacgata ctgcggggtt ggactggtgg
      301 gagtattgga tcaacattca tattccggca ttgcggcgct ggacttatcc gctgattgaa
      361 gggcgcccgc tggaagcgcg agcaccgcgg ctactgcaga tgccgcagcg caccgcagct
      421 gtaaccacaa ccgcggcaac aacgcccgaa aatggcgatg cgatgaagac tgggacgaac
      481 ggagcaacgt ggcgatattc ctgaccggtg gaaccggata catcggcgcg catgtcgccg
      541 ctaatttgct tgagcagcac gatgcggcgt tgaatctgct ggttcgtggc cgggatgcgc
      601 gtgatgccga gctgcgtctt tggaacgcgc tgcaactgca cctcccgttt ccgaagtttt
      661 acgaatatct gcaatcgaag atcagaattt ttcgcggcga tataaccctg ccgcagtttg
      721 gccttgatcc cgacagttac gaccggctcg ttcacactac cgattcagtt ctccattgcg
      781 cggcgtcgct caatcggaag tcggaaaaaa attgtctgaa tgtgaacctg cgtggaaccc
      841 tcgaagttgt gcaactggcg aggcgatcgc agtattatca cgggctgcgc cggttttcga
      901 atgtcagcac cgtagccgtc gcgggcaagc gcagccacga aattatttcc gaagatcgtt
      961 ccattgagtg ggaccgctcc gattacgacc cctatgcccg aaccaagaaa ttttgcgagc
     1021 acatggtgcg cgagctgctg ccggatgtgc cgctgacgat cttccgtccc agtatcgtct
     1081 tgggagacag ccggtatccg caaaccaccc agttcgatat ggtgcggtcg tttgtgtttc
     1141 tggcggaatt gccggtcctg ccatttcgcc cccacgacaa gatcgatatt gtgaatgtgg
     1201 attttgtggc ggcggcgatt tccactttgc atatgaaa
//
LOCUS       DATCBB010000267         3765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_72845, whole genome shotgun sequence.
ACCESSION   DATCBB010000267 DATCBB010000000
VERSION     DATCBB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72845"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VII37_03005"
     CDS             complement(<1..293)
                     /locus_tag="VII37_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5068721.1"
                     /translation="MPNFLELIFAQLQRADERVVLREIHGETFTSVTGRELLAQVQRG
                     RSYVRKFGLVPGDRCALLGGNSIQWVAVDLALMAEGIIVVPLYSRQAAAELAG"
     gene            complement(300..1739)
                     /locus_tag="VII37_03010"
     CDS             complement(300..1739)
                     /locus_tag="VII37_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY5068722.1"
                     /translation="MTDFILVAWVSALIGMARMKWMLGRGQRWKRGEKLRLLFAGYNG
                     TRNTGSDVRVNEMLHQIRHVLGAQNVDFSVMTQDFGRTKGYFDGTHQVHLPDVFPPML
                     YREVGKNHGVIACEGSMFKSKFANALTTMMIGSLGLASAENKLSVGYGGEAGHMDPLI
                     RNMCARYVSESLVITRNTESQTLLHSLGIQAELGTDTAWTFKPYPQAYAQKVLGEAGW
                     DGRTPILVICPIHPFVWPVKASIAKYAARVTTGAYKESQYRTVYFHESGHEVDRRFQN
                     YVSGITNATKAFLQRHQVFPILMAMERLDAVACREIAAQIPGTPVFTSDDYDMFQLVS
                     ILRACTYMVSSRYHGIVTCMPSLVASAGVTMDERIRNLMRERGHEHLFLTVDDPELEP
                     KLLEIMETLVREREEVREGIGRTVVRNLKVMARMGVLLEDAVRRQYPEFPLRSGVLSW
                     EDYLPPLDENLLQLVERYDATAAVLTANH"
     gene            complement(1736..2896)
                     /locus_tag="VII37_03015"
     CDS             complement(1736..2896)
                     /locus_tag="VII37_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5068723.1"
                     /translation="MAIFVTGSTGYLGSYLVAGLLREHRDMLNLLVRAKSEQEARERL
                     WKSLQLHFEFPEFSEYLKTRVRIFLGDLTNERFGLGDDSYHDLVDSTESIIHCAASLN
                     RKSERQCLNVNLRGALEVIQLARRAQNRNGLRRYSHVSTVAVAGRRQDEIVAEDASID
                     WARSDYDPYARTKKFGEHMVHQLLPDVPVTIFRPAIVLGDSRWPQTTQFDMVQAFDVL
                     AKMPVLPLRSEDRIDIVPANYVGKAIVTIHQKDAPAHTIYHLSSGVGSQTFRELTDAL
                     AAAGRTSRPSYWPWLGGPFSGMVEWLSRRGGATGYGASLLKVFWPYLNWNTVFDNSRV
                     VAELGGEAPAKFSTYAYPLLKFSRENKFKYPAKSWPAPASSPIPAIAGQVHA"
     gene            complement(2932..>3765)
                     /locus_tag="VII37_03020"
     CDS             complement(2932..>3765)
                     /locus_tag="VII37_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5068724.1"
                     /translation="GSDLPIAIVRPSIVESSERSPFSGWNEGINTSGPLSYLLGTNFR
                     QLPTNARKCLDVIPVDMVCRGMSLIAAAVIARCNARMYQLATSAVNPVNMGRSIELTG
                     LAHRKHYRMEQSVDNWLKVKFETIPVSKQRYERMSIPMQKAVVSRINRVAIALKMKKP
                     PLAKAERDLNRAEKLIELYEPFILHNDHVFECENTRMLSEALPPEEAGTFDFAPEAID
                     WWDYWINVHIPALRRWCYPLMEGRPLESRAPRELDWSSAPARAALSNSPAPSDPLWRS
                     S"
BASE COUNT          872 a         1179 c          907 g          807 t
ORIGIN      
        1 atgcctgcca actcggccgc cgcctgccgc gaataaagcg gcaccacgat gattccttcg
       61 gccatcaaag ccagatccac agccacccac tgaatcgaat ttcctcccag cagcgcgcag
      121 cggtccccag gcaccaaacc aaacttgcgt acatagcttc gcccgcgttg tacctgcgca
      181 agcagttccc tgccggtgac gctcgtaaat gtttcgccat gaatttcgcg caacaccaca
      241 cgctcgtcgg cgcgttgtag ttgagcaaaa attaattcca ggaaattagg catcgaggat
      301 cagtgattag cagtcaacac tgcggcagtc gcgtcgtaac gttccacgag ctgtaataaa
      361 ttttcatcca gcggaggcaa ataatcttcc caggacaata ccccggaacg cagaggaaat
      421 tccggatatt gccgccgcac cgcatcctcc aataaaactc ccatgcgcgc cataaccttc
      481 aggttgcgaa ccacggtgcg cccgattcct tcgcgcacct cctcgcgctc gcgcaccagc
      541 gtctccatga tttccaataa tttcggttcc agttccgggt cgtccacagt caaaaacaaa
      601 tgttcgtgtc cgcgctcgcg catcaaattt cgaatgcgct cgtccatggt cacgcccgcg
      661 gaagccacca aacttggcat gcacgtcacg atgccgtgat accgcgacga aaccatgtaa
      721 gtacatgcgc gcaaaatcga aaccaactga aacatgtcgt agtcgtcaga agtaaagacc
      781 ggcgtgcccg ggatttgtgc ggcaatttcg cggcaggcca ccgcatccaa tctttccatg
      841 gccattagaa tcggaaacac ctgatgtcgc tgaagaaacg ccttggtggc gttcgtgatt
      901 cccgaaacgt aattctgaaa tctccggtcc acctcgtggc ccgattcatg aaaataaacg
      961 gtgcggtact ggctttcttt gtaagcgccg gtggttaccc gcgccgcata ttttgcaatc
     1021 gacgccttta ccggccacac aaatggatgg atggggcaaa ttaccaggat cggcgtcctt
     1081 ccatcccatc ccgcttcgcc caaaactttt tgagcatatg cttgcggata cggcttaaat
     1141 gtccacgccg tatcggttcc caactccgcc tggatgccca gtgagtgcag caaagtctgc
     1201 gattccgtat tgcgcgtgat gaccagcgac tccgaaacat agcgcgcgca catgtttcga
     1261 atcagcgggt ccatgtgtcc agcctcgcct ccgtagccca cggacaattt attttccgct
     1321 gaggccaaac ccagcgatcc gatcatcatg gtggtcagcg cattcgcaaa tttgctcttg
     1381 aacatcgacc cttcacaagc gatcacgccg tgattctttc caacttcgcg ataaagcatc
     1441 ggaggaaaca catccggcag gtgcacctgg tgcgtgccat cgaaatatcc tttggtgcgc
     1501 ccgaaatcct gcgtcattac gctgaaatcc acattctgcg cacccaacac atgccgtatc
     1561 tggtgcagca tctcgttgac ccgcacatcg ctgccggtat tccgcgtgcc gttataccct
     1621 gcgaacagca agcgcaattt ttcgccgcgc ttccagcgtt gaccgcgacc aagcatccac
     1681 ttcattcgcg ccatgccaat cagcgcgctg acccacgcca ctaaaataaa atcggtcatg
     1741 cgtgcacctg cccggcaatc gccggaatcg gactgctcgc tggagccggc catgactttg
     1801 ccggatactt aaacttattt tcgcggctga atttcaacaa cggatacgcg tacgtcgaga
     1861 atttcgcggg cgcctcgcca cccagctccg ccaccacccg cgaattatca aacaccgtgt
     1921 tccagttcaa atacggccag aaaactttta gcaacgaagc gccatatccg gtcgcaccac
     1981 cacgtcggga gagccattcg accattccgg aaaatgggcc gcccagccac ggccaatagc
     2041 tcggccggct tgttcggcct gcggccgcca acgcatccgt caattcacga aacgtttgcg
     2101 agcccacgcc cgatgacaaa tgataaatcg tgtgtgccgg cgcatctttt tgatggatgg
     2161 tcacgatagc tttgcctaca taatttgccg gcacaatatc aatccggtct tcggaccgca
     2221 gcggcaatac cggcatcttc gcaagcacat cgaacgcctg caccatgtcg aactgtgtag
     2281 tctgcggcca gcgactatct cccagcacga tcgccggccg gaaaattgtt accggcacat
     2341 cgggaagcaa ctgatgaacc atgtgctcgc cgaatttttt ggtgcgcgcg tacggatcgt
     2401 aatccgagcg cgcccaatca atggacgcgt cttcggcgac aatctcgtct tgcctgcgtc
     2461 ccgcaaccgc cacggtcgag acatgcgagt agcgccgcaa tccattgcga ttttgcgcac
     2521 ggcgcgccag ttgtatcact tccaaggcgc cgcgcaaatt cacgttcaag cattgtcgtt
     2581 ctgatttgcg gttcagcgaa gccgcgcagt gaatgatcga ttccgtggaa tccactaaat
     2641 cgtggtaact atcgtcaccc aacccaaaac gctcattggt caggtcgccc agaaaaattc
     2701 gcacacgtgt ttttaggtac tcagaaaatt ccgggaattc aaaatggagt tgcagcgact
     2761 tccacaaccg ttcgcgcgct tcctgttccg acttggcacg caccaacagg ttcagcatgt
     2821 cgcggtgctc gcgcaaaagg ccggccacca aataacttcc caggtagccc gtcgagcctg
     2881 ttacgaagat agccatgtca ggaaaataac tgtgtcatcg gaacaagcat gtcaggaaga
     2941 tcgccataac ggatcagatg gcgctggcga atttgaaagc gccgcccgcg ccggcgcgct
     3001 ggaccaatcc agttcgcgcg gtgcccgtga ttccagtggg cgcccctcca tcagcggata
     3061 gcaccagcgg cgcagcgctg gaatatgcac gttgatccag taatcccacc agtcgatcgc
     3121 ttccggcgca aaatcaaacg tgccagcttc ctccggagga agcgcctccg acaacatgcg
     3181 cgtgttctcg cattcgaata cgtgatcgtt gtgcaaaata aacggctcat acagctcgat
     3241 caatttctcc gcgcgattca agtcgcgttc cgccttggcc agcggcggtt ttttcatctt
     3301 caaggcgatc gccacccgat tgatccgaga caccacggcc ttttgcatgg gaatcgacat
     3361 gcgctcatat ctctgcttgg acaccggaat cgtctcgaac ttaaccttca gccagttgtc
     3421 cacgctttgt tccatgcggt aatgcttgcg gtgcgccagt cccgtcagct caatactacg
     3481 gcccatattt acggggttca ccgccgaggt cgccagttga tacattcgcg cattgcaccg
     3541 cgcgatcacc gctgcagcga tcaggctcat cccgcgacac accatgtcta ccggaattac
     3601 gtccaggcat ttccgtgcat tggtgggcag ttgccggaaa tttgttccca gcaaatacga
     3661 aagcggcccg gaggtattga ttccttcatt ccaacccgaa aacggcgaac gctccgagct
     3721 ttccacaatc gacggccgca caatcgcaat cggtaaatca ctccc
//
LOCUS       DATCBB010000268         7217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_268165, whole genome shotgun sequence.
ACCESSION   DATCBB010000268 DATCBB010000000
VERSION     DATCBB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268165"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VII37_03025"
     CDS             complement(<1..1122)
                     /locus_tag="VII37_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5068725.1"
                     /translation="MSAIDSQSSQRVTPSNSPQVVVESLVKQYGPLRALDDVTFSVAP
                     GEWVALMGPSGSGKTTLINILGGLDTLTSGRVIVDDVDLSKRTESQLVRYRSEKVGFV
                     FQQFHLIPYLSALENVMLAQYFHSVTDEKQAQDALQRVGLGDRLTHLPAQLSGGEQQR
                     VAIARALINQPKLILADEPTGNLDEANEALVIDIFRALHNAGHTILMVTHDPDIARQA
                     GRRIELTHGHLSLDTTQTDAAISIDDEIRFDHLLERIWMCGEEGTNARLDSIRIPGAP
                     GELDSPRTLGRMADLKLVEVRDTDVHLTETGNRRARDVVRRHRLAERLFKDTFAIDDS
                     EAHTQACKFEHIISPELDQRICTFLGHPKTCPHGNPIPPG"
     gene            complement(1119..2267)
                     /locus_tag="VII37_03030"
     CDS             complement(1119..2267)
                     /locus_tag="VII37_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068726.1"
                     /translation="MPDRKMFWRIVRRLFAAQRGRLLVILLALGASAALTAALLNLQV
                     DARDRLTKEFRVFGANIIVAPRDSKSSDAGVVTLDESTVERVRQSLPPHASADSYLYL
                     VASVSAANSAQPVNAVIAGKRTVQIDQSSTHATGATPLDNKNICSVGAKAAQQLHLQP
                     GDRLTLRDEGREETCTSEIADARGDAEDSQIVVNLDAAQRLASMPGRVSLIQVSVAGT
                     PREIRDDIATLAQNIPAADVHGLRQFTDAEAKIYTRVSGLLTATTALVLALTGLCVMA
                     AMTNVAMERKNDVGLMKAIGGATRRVLRLFLAEAALLGLAGGIVGAAAGLLLSIWLGK
                     TVFGVAARPRLIVYPVSVALTILVAIVSSYPLRRLANISPASVFRGEA"
     gene            complement(2268..3551)
                     /locus_tag="VII37_03035"
     CDS             complement(2268..3551)
                     /locus_tag="VII37_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068727.1"
                     /translation="MFLRLVADSFGRRPRRKLLTGAALALGMAVVTAAFSVSLDVGDR
                     LAHEFRALGANLLITPQADSLPLEIGGVDYRPVNSGSYLPEHDLGKIKTIFWRNNIVG
                     FAPVMEVPAEVSLSTASSPLKATLVGTWAHHSIALPDGRSYATGMDQTNSWWHVDGRW
                     FHDDATECIVGKSFATRTNIKPGDTLHINSADRSIDLTVTGILSAGGPEDESIVVPLQ
                     IAQQLANKPGQYRRLYVSALTKPEDDFARRNPSTMNAADLERWSCSPYASSIAYQIKQ
                     VLPGVDAHVIRRVAEGEGSILTRVQTLLWLASFAALLAAALAVGASSAASVIERRSEI
                     GLMKAIGAGSGTIGFLLAAEQLLLAFVGGGVGYALGIILARVLGEKIFGVAPEPSILV
                     FIVILTLAGGVTLLGSAIPLHRASRYEPAPILRGE"
     gene            complement(3575..4900)
                     /locus_tag="VII37_03040"
     CDS             complement(3575..4900)
                     /locus_tag="VII37_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HEY5068728.1"
                     /translation="MHASVHWISVANDSQLEIGVSLLAAFLIALREGVEASLVVGIIL
                     VYLSRTGRAHLARFVWFGAAAAAALSLAVAIALENWHINQDGFEGLLMLVAAFFVITM
                     IVWMNHVARHLKNEIEKKIEAYAARAGTAAGWGIFLFVFLMVLREGAELALILRAVEL
                     SSAGLQTWIGTLVGIAGAVAVGLFFFKGTLRVPLHRFFAVTSVILMLVAFQLALTGLH
                     ELSEAQWIPSSKTEMAIIGPVVRNELFFFVLIFGAAALLVLREWQSASSKDASTPPTG
                     EAGKRLLESQNRRQRNWMMAAATACLVVILVLTADFIYVRANSAPPSSTPVTAVGQEV
                     RVPISQVDDGKLHLFTVNVANQALRFMIIKKPDGYGTALDACRICGAEGYRQDGQNVI
                     CRHCGSAIYVPSIGDQGGCNPINVASHIDGSDIVMDLSSLGHAAQEVPR"
     gene            4966..>7217
                     /locus_tag="VII37_03045"
     CDS             4966..>7217
                     /locus_tag="VII37_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY5068729.1"
                     /translation="MMRVTQRLSGSIFLSALLFLAQCGAGNSAWGATRSGEQEEARPA
                     KGDVPLHVDLKRAQKAAERGDQAEAAGHIDEALLHYQEAAHFAPRDARYVAKEAALRS
                     RIVRSHVDIAERAALEGRMEKATEELRKALHIDPGNAMVAERLTQMNSMEEEPPNQAQ
                     VEMTGLPKLQPKSGRQDVDIQGETRSVYEELCARFGIKASFDPDVAGRQVRLRVENVD
                     FYTALMVLGTQTGTFWRALNPTLIFVTTDSPQKRKDYGPEGQQVFPLPAAVAPDEMTE
                     LLRVLRDITGATHLQLDTRSRTITMRDTPERLAVAGQLIQEVEKARGEVLLDIELLEV
                     DRATLQKLGVTPPTSVSLIYLTPNAVRTLASSTDLANLLTNAQQIFTGQGFSSIPPFI
                     LLGGGLSTFLLTTPGATVDFSDSLSLVRSGREVLLRAQDNKPATFFVGDRYPVTLSLL
                     SNSLGTGGTFTPSPTGAVFPQTNFAVGNNPTALVAAPFTGGTLPDLAVVFDNIGTNTF
                     AILQNQDNGNFVQVSPAPVALGTNETGQVAIGTGIFRTDSTKFTTAQPHDVILVNSTS
                     NNVSVLLGNVDANGVPNGTFTEAPGSPIAVGKNPSSIIVADFNGDGIQDFAVTNKDDN
                     SISVFRGLGDGTFRQFRGSPFLLNDGNVRETGPVAMVAGTFRQGDMTDLAVVNQTSNN
                     ISILLDTIDPDTEIRFTEAPNSPIAVGTSPVAIATGDLNADGVGDLVVANQGDNTLSI
                     LLGSTTIDGT"
BASE COUNT         1640 a         1826 c         2318 g         1433 t
ORIGIN      
        1 tccgggcgga atgggattgc cgtgcggaca agtttttggg tgaccgagaa aagtgcagat
       61 gcgctggtca agctcggggc tgatgatgtg ttcgaatttg caggcttggg tgtgggcctc
      121 ggaatcatcg atggcgaagg tgtctttgaa gagacgctct gcaaggcgat ggcgccgcac
      181 cacatcgcga gcgcgacgat tgccagtctc ggtgagatga acatcggtgt cgcgcacttc
      241 cacgagcttc agatcggcca tgcgtcctag tgtgcgaggg gaatcaagtt cgccgggcgc
      301 cccgggaatt cgaatgctgt cgaggcgcgc gttggtgccc tcttcgccgc acatccagat
      361 gcgctccagc aaatgatcga agcgaatttc gtcgtcaatg gaaatggcgg cgtccgtttg
      421 cgtggtgtcg agacttagat ggccgtgcgt aagttcgatg cgacggccag cctggcgggc
      481 aatgtctgga tcgtgagtga ccatcaggat ggtgtggccg gcattgtgca gtgcgcgaaa
      541 aatatcgatg accagggctt cgttcgcttc atcgagattt ccggtgggct cgtcggcgag
      601 aattaatttt ggctggttga taagcgcgcg agcaatggcc acgcgctgtt gttcgccgcc
      661 ggataattgc gcgggaagat gcgtgagacg gtcgccgagg ccgacgcgct gtaaggcgtc
      721 ttgggcttgc ttctcgtcgg tgacgctatg gaaatattgc gcgagcatga cattttccag
      781 tgcggaaaga taaggaatga gatgaaattg ctggaagacg aagcctactt tttcggagcg
      841 atagcgaact aactggcttt cggtacgctt ggagaggtcg acatcgtcaa cgatgacgcg
      901 gccggaagtt aaggtgtcca gtccgccgag aatattgatc aaggtggttt ttccggaacc
      961 ggatggtccc atgagcgcga cccattcgcc aggagcaacg ctgaaggtga cgtcatcgag
     1021 agcgcgtagc gggccgtact gcttaacaag cgactcgacc acaacttgtg gcgaattcga
     1081 cggcgtgacc cgttgcgacg attgtgaatc gatcgcgctc acgcttcccc ccggaaaacc
     1141 gaggcgggcg aaatattggc tagacgccga agcggataag agctgacgat ggcgaccaga
     1201 attgtgaggg ccacagaaac aggatacaca atgagccgcg gacgtgctgc cacaccgaat
     1261 acggttttgc caagccagat ggaaagtaag aggccggcgg ccgcgccgac aatgccgccg
     1321 gcgaggccga gcagcgcggc ttccgcgaga aacaaacgca gtacgcgacg cgtggcacca
     1381 ccgatagctt tcatcaagcc cacatcattt ttgcgctcca tggcgacgtt ggtcatggcg
     1441 gccatgacgc agaggccggt gagggccagc acaagcgcgg ttgtggcggt gagcaatccg
     1501 ctaacgcgag tataaatttt ggcttcggca tcggtgaact ggcgcaagcc gtggacgtct
     1561 gcggcgggaa tattttgtgc gagcgtggcg atgtcatcgc gaatttcacg gggagtgccg
     1621 gccacgctta cttgtatgag gctgactctg ccgggcatgg aggcgagacg ttgggcggcg
     1681 tcgagattca cgacgatctg gctgtcctcg gcgtcgccac gggcgtcggc aatttctgag
     1741 gtgcaggttt cttccctgcc ttcgtctcgc aacgtgaggc gatcgcccgg ttgcagatgt
     1801 aattgctgcg cggctttggc gcctaccgag caaatatttt tgttgtcgag cggagtagca
     1861 ccggtggcgt gtgtggatga ctgatcgatc tgcacggtac gcttccctgc tatgacggca
     1921 ttcactggct gagcggaatt cgccgcggac acgctggcca ccaaataaag ataggaatcg
     1981 gccgaggcgt gtggtggtag cgattgacga accctttcga cagtggattc gtccagcgtg
     2041 acaacgccgg cgtcagaaga tttactgtct cgtggagcta cgattatatt ggcgccgaag
     2101 acgcgaaatt ctttggtgag ccggtcgcga gcatccactt ggaggttcag gagggcggcg
     2161 gtcagcgccg cgctggcgcc gagcgccagg agaattacga gcagccggcc gcgctgggct
     2221 gcgaagagac ggcgaacaat gcgccagaac attttgcgat cgggcattta ttcgcctcgc
     2281 aaaatgggcg ctggctcata gcgcgaagcg cgatgcagcg gaatggcgct gcccaaaaga
     2341 gttacgcctc ccgcgagggt caggatgacg atgaagacca ggatggaagg ttctggagcc
     2401 acgccaaaga ttttttcgcc gagaacgcgc gcgagaatga tgccgagagc gtaaccaact
     2461 ccgcctccga caaatgcgag caggagttgt tcggcggcga gaagaaaacc gatggtgccg
     2521 gaaccggcgc cgatggcttt catgaggccg atttcggagc ggcgctcgat cactgaggcg
     2581 gccgaagatg cgcctacagc gagtgcggcg gcgagcaagg cggcgaagga tgcgagccag
     2641 agaagtgttt gcacgcgtgt gaggatgctg ccttcccctt cggccacacg gcgaattaca
     2701 tgcgcgtcaa caccgggcaa aacctgtttt atctgataag cgatgctcga ggcgtacgga
     2761 ctgcaggacc aacgctcgag atccgcggcg ttcatcgtgg aaggattgcg gcgcgcgaag
     2821 tcatcttccg gttttgtcag cgcgctgacg tagagacggc ggtattgacc gggcttgttc
     2881 gcgagttgct gggcgatttg cagcggcacg acaatggatt cgtcttcggg gccgccggca
     2941 gaaagaatgc ctgtaacggt taagtcgatg ctgcgatctg ctgaattgat atggagcgtg
     3001 tcgccgggct tgatgtttgt tcgagttgcg aaggacttgc cgacgataca ttcggtcgcg
     3061 tcgtcatgga accagcggcc gtcgacatgc caccaggaat tggtttgatc cattccggtc
     3121 gcgtaactgc gtccgtctgg aagggcaata gagtggtgcg cccacgttcc gaccagcgtg
     3181 gctttcagcg gagatgaggc agtcgagagc gacacttcgg cgggaacttc cattacaggt
     3241 gcgaagccga cgatattgtt gcgccagaaa attgttttta ttttgcccaa gtcgtgctcg
     3301 ggaaggtatg agccggaatt cacggggcga taatcgacgc cgccaatttc gaggggaagc
     3361 gagtcggcct gcggagtgat gaggagattg gcgccgagag cgcgaaattc atgcgcgaga
     3421 cgatcaccga cgtcgagcga aacgctaaag gccgcggtga cgacggccat gccgagagca
     3481 agagccgcgc cggtgagcaa cttgcggcgg ggacggcgtc cgaacgaatc ggcaactagg
     3541 cggaggaaca taatggcgga tgcgcgttcc gcagctaacg cggaacctct tgagcggcgt
     3601 gacccaggct ggaaagatcc atcacgatgt cgctgccatc gatgtgcgag gcaacgttaa
     3661 tgggattgca gccaccttga tcgccgatgg acggaacgta gatggcggag ccgcaatgac
     3721 ggcagataac gttctggccg tcctgccgat atccttccgc gccacagatg cggcaagcat
     3781 cgagcgctgt gccgtagcca tcgggcttct tgataatcat aaaacgcaaa gcctggttgg
     3841 cgacgttcac ggtgaaaaga tgtaattttc catcgtccac ttgtgaaata ggaacgcgaa
     3901 cttcctggcc tactgcggtg actggcgtgg aggacggagg ggcagaattg gcgcggacgt
     3961 aaataaaatc ggcggtgagc acaagaatta ccaccaggca cgcagtagca gcggccatca
     4021 tccagttgcg ttggcggcga ttttgcgatt ccagcaggcg ttttcccgct tcaccggttg
     4081 gtggagtgga agcgtctttg cttgaagcac tctgccactc gcgcagaacg agcaaggcgg
     4141 cggcgccaaa aatcaagacg aagaaaaata attcgttgcg aacgaccggg ccgatgatgg
     4201 ccatctcagt tttgctggac ggtatccatt gggcttcgct gagttcgtgt aagccggtga
     4261 gcgcaagttg aaaagcgacc agcatcagaa tgacgctagt caccgcgaag aaacgatgca
     4321 gcggcacgcg caaggtgcct ttgaagaaaa atagtccaac ggcaaccgcg ccggcaatac
     4381 ctactaacgt gccgatccag gtctgcaggc ctgcactgga gagttcaacg gcgcgaagga
     4441 tcaacgccag ctcagcgcct tcacgcaaca ccatcaagaa aacgaagaga aagatgcccc
     4501 atcccgcggc ggtgccggca cgcgcggcat aagcctcgat ttttttttct atttcatttt
     4561 tcaagtggcg cgcaacgtgg ttcatccata cgatcatcgt aatgacgaag aaggcagcaa
     4621 ccagcatcag gaggccttca aagccgtcct ggttgatatg ccaattttca agggcgatgg
     4681 cgacggccag gctcagagcc gcggcggcgg cggcgccaaa ccaaacgaag cgtgcaagat
     4741 gggcgcgacc ggtgcgggac aaataaacga ggataatgcc tactacaaga gaggcttcga
     4801 caccttcacg gagagcgata agaaaagccg cgagcaacga gactccaatc tccagttgtg
     4861 aatcatttgc aactgagatc cagtgtacgc tcgcgtgcaa ttagggtcaa gccacgcgca
     4921 ttgctgtaga tacgtcacgg tagaatggat ggaccaactt taacgatgat gcgtgtgaca
     4981 caacgactgt ctggctcgat cttcctgtct gcgctgctat tccttgcgca atgtggagcg
     5041 gggaattccg cgtggggcgc cacccggagc ggggagcagg aagaggctcg gccggcgaaa
     5101 ggtgatgtgc cgctgcatgt cgatttgaaa cgcgcgcaga aggcagctga gcgcggcgat
     5161 caagcggaag ctgcggggca catcgatgaa gcgttgctcc attatcaaga ggcggcgcat
     5221 ttcgcgccga gggatgcgcg ctacgtcgca aaagaagcgg cgctgcgttc aagaattgtg
     5281 cgctcgcatg tggatatagc ggagcgcgcg gctctcgaag gacggatgga aaaggccact
     5341 gaagagttgc gcaaggcgct gcacatcgat ccaggaaacg cgatggtggc ggaacggctt
     5401 acgcagatga actcgatgga ggaggaacct cccaaccaag cacaagtgga gatgacgggg
     5461 ctgccaaagt tacagccgaa gagcggtaga caggatgtgg atattcaagg agaaaccaga
     5521 tcggtttatg aggaattgtg cgcgcgattt ggaataaagg catcgtttga tccggatgtg
     5581 gcgggccgac aggtgcggtt gcgggtggag aacgtagatt tttataccgc gctgatggtg
     5641 ctggggacgc agaccggaac gttctggcgc gcgctgaacc ctacgttgat ttttgtgaca
     5701 acggatagtc cacaaaagcg caaagactat ggcccggaag ggcaacaagt gttcccgttg
     5761 ccggcggcgg ttgcgcccga cgaaatgacg gaactgttgc gcgtgttgcg agatattacg
     5821 ggcgcgacgc accttcaact ggacacgcga agccgcacga ttaccatgcg cgacacgcca
     5881 gaaagattgg cggtggcggg acagttgatt caagaagtgg agaaggcgcg cggagaagtt
     5941 ttgctggaca tcgagttgct ggaagtggac cgcgccacgc tgcaaaagct gggagtgacg
     6001 ccgccaacct ccgtctcgct gatttacttg acaccgaacg cggtgcgaac gctggcaagc
     6061 tccacggacc tggcgaattt acttaccaac gcgcagcaaa tattcacggg gcaaggattc
     6121 tcgagtatcc cgccatttat tttgttgggc ggcggattgt ccacattttt gctgaccact
     6181 ccgggagcca cggtggactt ttcggattcg ctctcgctgg tgcgcagcgg gcgagaagtt
     6241 ctgctgcgcg cgcaggataa taagccggca acgttctttg tgggggaccg ctacccggtt
     6301 acgttgtcgc tgctgtcgaa cagtttgggc accggcggaa cctttacgcc ctcgcctacc
     6361 ggagccgttt ttccacaaac gaatttcgca gtggggaaca atcccacagc gctggtggct
     6421 gcgccgttta ccggtggaac gctgccagac ctggccgtgg ttttcgataa tattgggacg
     6481 aatacgtttg ccattttgca aaatcaggat aacgggaact tcgtgcaggt atctccggcg
     6541 ccagtggcgc tcggcaccaa tgagaccgga caggtagcga tcggcacggg aatttttcgc
     6601 acggacagca cgaagtttac gactgcgcaa ccgcatgatg taattttggt gaattccacg
     6661 tcaaataacg tttcggtttt gctgggcaat gtagacgcca acggcgtgcc caacggaaca
     6721 tttacggaag ctccgggatc gccgatcgcg gtgggaaaaa atcccagctc gattatcgta
     6781 gcggacttta acggagatgg gattcaggat tttgcggtga ccaacaaaga tgacaattcc
     6841 atctctgttt ttcggggact aggcgatgga acgttcaggc aatttcgggg gtcgccattt
     6901 ttgctgaatg atgggaacgt cagggaaacg ggcccagtgg cgatggtggc gggaactttc
     6961 cggcaaggag atatgacgga tctggcagtg gtgaaccaga cttccaataa tatatcgatt
     7021 ctgctggaca ccatcgatcc agataccgaa ataaggttca cggaagcgcc gaattcacca
     7081 atcgcagtag gaacgtcgcc ggtcgcgatt gccaccggag atctaaacgc agacggcgtg
     7141 ggcgatctgg tagtggcaaa tcaaggtgac aacacgctat cgattttgct ggggagtacg
     7201 accatcgacg gaacgtt
//
LOCUS       DATCBB010000269         1517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_203191, whole genome shotgun sequence.
ACCESSION   DATCBB010000269 DATCBB010000000
VERSION     DATCBB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203191"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VII37_03050"
     CDS             <1..583
                     /locus_tag="VII37_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5068730.1"
                     /translation="AGRLVVEDLDSLNGTFVNDMRVERATLHDGDSIHIGKHHIKVNA
                     TADTPLQQGGSVQQKAAAPRINETMVLDTKVRREMLQQAAAMGERTQFASGRLKVPTL
                     VVLGGRTDRKEYTLSGKLTVIGKSNLATVQLRGWFKPAMAAQINQRDDGYYIGAGSKV
                     PSVNGSPIRGQVLLKDGDLINVSGVRLNFIFRD"
     gene            595..>1517
                     /locus_tag="VII37_03055"
     CDS             595..>1517
                     /locus_tag="VII37_03055"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY5068731.1"
                     /translation="MEASSGVTRRARGLAVVLELLQTWWQHRVSFLISLAITITALGL
                     YVFTYIGEHSNPLLVFVERLELDALDTRFRMRPAKYSSPDPRIVIVDIDQRSQEGLGK
                     WPFSRTNFATMLDVLRADGAKVVGFDVTFDKPDQTAAPVRALADQLEKRKTAGQRVDP
                     RLQEEITALAAEYDADKQFGAAIQKFGNVVLGNFFFPREELKGTDEATLNSYAELVQW
                     YSLGQNSLNPATGKQDFLDLMHRYDANGILYTATMANIPALLSPDNNSKSTMGFFNVL
                     PDADGVLRRSLLAVPFDPSGKLAEAELYGSL"
BASE COUNT          322 a          437 c          472 g          286 t
ORIGIN      
        1 ggcgggccgg ctggtggtcg aggacctcga cagcctgaac gggacgttcg tgaacgatat
       61 gcgcgtggag cgcgccacgc tgcacgacgg cgactccatc cacatcggca aacatcacat
      121 caaggtgaac gccacagcgg atactccgct gcagcaaggc ggcagcgtgc agcaaaaggc
      181 cgcggctcca cgcatcaatg aaaccatggt gctggatacc aaagtgcgcc gcgagatgct
      241 acagcaagcc gcggcgatgg gtgagagaac acaatttgct tccggccgcc tgaaagttcc
      301 cacgctggtg gtgttgggcg gcagaacgga ccgcaaggag tacacgctga gcggtaaatt
      361 gacggtcatc ggcaaatcca acctggccac cgtgcaactg cgcggctggt tcaagccggc
      421 gatggctgcg caaatcaacc agcgcgacga cggttactat atcggcgcgg gctcgaaggt
      481 tccttccgtg aatggctcgc caatccgtgg gcaagtgctg ttgaaggacg gcgatttgat
      541 taacgtcagc ggcgtgcgcc tgaactttat tttccgcgac tgacgcgaca gtcgatggaa
      601 gcttcgtcag gcgtaacccg gcgggcgcga ggattggcag ttgtgctgga gctgctgcag
      661 acctggtggc agcaccgcgt ttccttcctg atcagcctgg ccatcaccat caccgcgctg
      721 ggcctgtacg ttttcactta catcggcgag catagcaatc ccctgctggt tttcgtcgag
      781 cggctggaac tggatgcgct ggatacgcgc ttccggatgc gccccgcgaa atatagttct
      841 cccgaccccc gcattgtaat cgtcgacatc gatcaaaggt cgcaggaagg gcttggcaag
      901 tggccatttt cgcgcacgaa ttttgcgacc atgctggacg ttttgcgcgc ggacggcgcg
      961 aaggtggtgg gcttcgacgt tacatttgat aagccggacc agaccgcggc gccggttcgc
     1021 gcgctggccg accaactgga aaaacgcaaa accgcaggcc agcgcgtaga tcccaggctg
     1081 caagaggaaa tcaccgcgct ggcggcggaa tacgacgccg acaaacagtt tggcgcagcg
     1141 atacaaaagt tcggcaacgt ggtgctgggc aattttttct ttccccgcga ggaattgaag
     1201 ggcaccgacg aggccacgct gaattcctac gccgaactgg tgcagtggta ttcgctggga
     1261 caaaattcgc tgaatccggc gaccggcaag caggattttc tggatttgat gcaccgctat
     1321 gacgccaacg gcattctgta taccgcgacg atggcgaata ttcccgcgct gctcagtccc
     1381 gacaacaaca gcaagagcac catgggcttt ttcaacgtac tgcccgacgc cgatggcgtg
     1441 ctgcggcgtt cgctgctggc cgtgccgttc gacccctcgg gaaaattggc ggaggccgag
     1501 ctgtatggat cgctgga
//
LOCUS       DATCBB010000270         1007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_170827, whole genome shotgun sequence.
ACCESSION   DATCBB010000270 DATCBB010000000
VERSION     DATCBB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170827"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..775)
                     /locus_tag="VII37_03060"
     CDS             complement(71..775)
                     /locus_tag="VII37_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY5068732.1"
                     /translation="MTFQNNFAPPNRAPRSYPRRSSALGLLLLIAIIFCFFYLGRWLV
                     VEDPLDHAQAIAVLSGRMPVRVLEAAEIYRAGYAPEVWLTHSTEPGKSLQEMGVKYLG
                     EEDYNREILIHEGVPPAAIRVLEPPILNTADEIVAIKFALNSAPLHTVIVVTSKPHTR
                     RVHTLWRALTHGEGRIIVRAASQGPFDAQHWWRSTTDALDVVREVLGLLNAWAGLPLS
                     HSESPPPITGPRNTRP"
BASE COUNT          213 a          225 c          358 g          211 t
ORIGIN      
        1 ccccggaagc gatttttatt tttttaggcg ggcacgcgct cggcgaggcc gggtaaaaag
       61 gtgctggtaa ctacggacgc gtatttcggg gcccggtaat tgggggcggt gattctgaat
      121 ggctgagcgg caggccagcc caggcgttca gcaggccaag aacttccctg actacatcca
      181 aggcgtcggt ggtgctgcgc caccaatgct gcgcgtcaaa gggaccctgt gatgcggcgc
      241 ggacgatgat gcggccttcg ccgtgggtaa gggcgcgcca cagggtgtga acgcggcgcg
      301 tgtgcggttt gctagtgacg acgattacag tatgaagagg cgctgagttc agggcgaatt
      361 tgatggcgac gatttcgtcg gcagtgttga gaatgggcgg ctctagtaca cggatggcag
      421 cgggcggaac gccttcgtga atcagaattt cgcggttgta atcttcttcc ccaagatatt
      481 tcactcccat ttcttgaagt gatttgccgg gttcggtgga atgggtgagc cacacttccg
      541 gagcgtagcc tgcgcgataa atttccgctg cttccaagac gcgcacgggc atacggccac
      601 ttagaacggc gatggcttga gcatggtcga gaggatcttc cacgacgagc cagcggccga
      661 ggtaaaagaa acagaaaatg atggcgatca ggagaagaag gcccagagcg ctggaacggc
      721 ggggatacga gcgcggcgca cgatttgggg gtgcaaaatt attttgaaaa gtcatgaggc
      781 ggaggcatcg tatcttgcga tggcgaggtg gtcaatcgac ggagcgcagg ctacactgtc
      841 gaatcacaat tttggaggat gaatgcgcgc ggtggtgacg ggtggagcgg gatttctggg
      901 gtcgcatttg tgcgaccggt tgcttgcgga aggctgggaa gtgttggcgg tggataattt
      961 cattaccggg gcggcggaaa atgttgggca tctggcgggg aacgcga
//
LOCUS       DATCBB010000271         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_430455, whole genome shotgun sequence.
ACCESSION   DATCBB010000271 DATCBB010000000
VERSION     DATCBB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430455"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /gene="metG"
                     /locus_tag="VII37_03065"
     CDS             <1..1520
                     /gene="metG"
                     /locus_tag="VII37_03065"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEY5068733.1"
                     /translation="ENYFFQLSKFEKPLLNYYEQHPEFIQPEARRNEVISFVRGGLRD
                     LSISRTTLKWGIPWQGDEKHVFYVWSDALTNYITAVGYPLRKDEFARYWPADVHLVGK
                     EIIRFHAVYWPAFLMAAGLDLPKQIFAHGHLLFQDEKMSKSRGNIQSAQPIVSVLGVD
                     ALRYFLLREIVFGGDGGFSREALIARYNSDLANGLGNLASRTAAMIERSFGGKIPKAA
                     DLQPQDAELVKVAQTSIGEVLEHYDRFEFSRALELIWSIIAAADKYLTTEQPWSLGTS
                     DADQQRRGTILWLTAEVLRIVTALAHPVLPESTAKVWKLLGQTGNIASLELDNLRWGQ
                     LLPGGKLGELQPLFPRVEKNEAMERIEAMENQSQKSAAAPAPAAPATAPTAAAAPAAA
                     PASTEKIGIEDFAKVEMRVGQIQTAERIAGADKLLKLTVDIGTEVRQICAGIAQFYEP
                     EKLIGRKVAVVTNLAPRKLRGVESNGMIVAASVGPEGRPVLAGFLEDVEVGARLK"
     gene            1533..2342
                     /locus_tag="VII37_03070"
     CDS             1533..2342
                     /locus_tag="VII37_03070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY5068734.1"
                     /translation="MELVDSHAHIDFPQFADDREAMLQRARVAGVSALLAIGTGPGPE
                     KLDSALPYADQYDWIYASIGIHPHEAKQATPQHLEELSRLAKHPKVIAWGEIGLDYFY
                     DHSPRDIQHQVFRDQMALARDAKLPIILHCRDAWPDCLALIEEVWRPTGIGGILHCFT
                     GTLDEAHRGLDAGFMISFAGNSTYPKTQHIRDVAKEIPLDRILIETDSPFLAPQPYRG
                     KRNEPAYVAEVAHTLANVRNLSPEEFAASTAANFRRFFHLVRPSESPAATF"
     gene            2503..3375
                     /locus_tag="VII37_03075"
     CDS             2503..3375
                     /locus_tag="VII37_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5068735.1"
                     /translation="MTSYLLGGGGKRLRPALLILCNRYAGANSPGAIRLAAVVELLHS
                     ATLIHDDVIDSANTRRGRPSANSRWGNHRSVLAGDWLYMQSFQMALEERNFRILDVLI
                     DLTQKMVEGELIQLEKIGRIDVTEDDAVRLATYKTACLFSGCARLGAVLAGLEGEEEE
                     ALADYGRYAGLAFQLVDDLLDFTASAKQLGKPVLSDLKEGKVTLPLIYAMENGHRDAR
                     QLVAQVLEEKEFNSVRPETLVALVHDSGALGRARKLAHDYARRAKACLNGHSDSEYGR
                     ALMTLPDFILAREN"
     gene            3391..>3914
                     /locus_tag="VII37_03080"
     CDS             3391..>3914
                     /locus_tag="VII37_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068736.1"
                     /translation="MLPFKAVYHPRYDLNLGAHVFPSQKFRLVSESLVSSGIADASDF
                     LRPEPASDADLLRVHTASWIRKLRTGTLTASEIMKLEVPYSKELAEAVWLAAGGTILA
                     AQSALRDGFGSNLSGGFHHAYANHGEGFCAIHDVAVAVRRLQADGAIKKAIIVDTDVH
                     HGNGTAAIFSGDSSV"
BASE COUNT          889 a         1194 c         1020 g          811 t
ORIGIN      
        1 aagaaaatta ttttttccag ctttccaaat ttgaaaagcc gctcctcaat tattacgaac
       61 aacatccgga gttcatccag cccgaagctc gccgcaacga agtaatttca ttcgtgcggg
      121 gcggtttgcg cgatctctcc attagccgca ccacgttgaa gtggggaatc ccctggcagg
      181 gcgacgaaaa acacgttttc tacgtatggt ccgacgcgct gacaaattac atcaccgcgg
      241 tcggctaccc acttcggaaa gacgaattcg cccgctattg gccggcagat gtccatctcg
      301 tcggcaagga aattatccgt tttcacgccg tctactggcc ggccttccta atggctgcgg
      361 gtttggatct gccaaaacag attttcgctc acggccacct tttattccag gacgagaaaa
      421 tgtccaaatc gcgcggcaac attcaatccg cgcaacccat cgtcagcgtc ttgggcgtcg
      481 acgcattacg ttacttcctg ttgcgtgaaa ttgttttcgg cggagacggc ggtttcagtc
      541 gcgaagctct gatcgctcgc tacaacagcg atctcgccaa cggcttgggt aaccttgcca
      601 gccgcacagc cgccatgatc gagcgcagtt ttggcggaaa aattcccaaa gccgctgacc
      661 ttcagccaca agacgctgag ctcgtcaaag tcgcgcaaac ctccatcggc gaggttctcg
      721 agcactacga cagattcgaa ttctctcgcg cgctcgaatt gatctggtcc atcattgccg
      781 ccgccgacaa atatctcacc actgaacagc cctggagcct gggcacttcc gatgcggacc
      841 agcaacgtcg cggaacaatt ctgtggctca ccgccgaggt tttgcgcatc gtaacagcgc
      901 tggcgcatcc agtgctcccg gaaagcaccg ccaaggtctg gaagctcctg ggacaaactg
      961 gaaatatagc atcgctggaa ttagacaacc tgcgctgggg ccaacttttg cccggcggga
     1021 agctcggaga acttcagcct ttgttcccgc gcgtcgaaaa aaacgaagca atggagagga
     1081 tcgaagctat ggaaaatcag tcacagaaat cggccgccgc gccagcacca gctgcgcccg
     1141 caactgcacc cactgcggca gccgcaccag ccgcggcacc cgcgtctacg gaaaaaatcg
     1201 gtattgaaga ctttgccaaa gtcgaaatgc gcgtcggaca gattcaaacc gccgaacgca
     1261 tcgcgggcgc agacaaattg ctcaaactca ccgtggatat tggcacggaa gttcgccaaa
     1321 tctgcgctgg catcgcccaa ttttacgagc cggaaaaatt gatcggccgc aaagtcgccg
     1381 tagttacgaa tctagctcca cgcaaacttc gcggtgtcga atccaacggc atgatcgtcg
     1441 ccgcgagtgt cggcccagag ggcagaccgg ttctggcagg tttcctggaa gatgtggaag
     1501 ttggagcccg cctcaagtag catccactgc gaatggaact agtcgactcc cacgcccaca
     1561 tcgattttcc ccaattcgcg gatgaccgcg aagcgatgct gcagcgggcc cgcgttgcgg
     1621 gagtctcggc gcttctcgcc attggcaccg ggccaggacc agaaaaattg gattccgcgc
     1681 tgccatacgc ggatcaatat gactggattt acgccagcat cggcatccat ccccacgaag
     1741 ctaagcaagc cacgccccag caccttgaag aactctcgag actcgcaaag catcccaaag
     1801 tcatcgcgtg gggcgaaatt ggtctggatt atttttacga tcactcccca cgcgacatcc
     1861 agcaccaggt atttcgcgac caaatggcgc tcgcccgcga cgccaagctc cccataatcc
     1921 tccactgtcg cgacgcctgg cccgattgcc tcgcgctcat cgaagaagtc tggcgcccca
     1981 caggaatcgg cggcatcctg cattgtttca ccggcacgct ggacgaagcc caccgcggac
     2041 tcgacgccgg cttcatgatt tccttcgccg gcaactctac ttatccaaag acccagcaca
     2101 tccgcgacgt cgcaaaggaa atccctctcg accgcatcct gatcgaaacc gactccccat
     2161 tcctagctcc tcagccctac cgcggcaaac gcaacgagcc cgcctatgtc gcggaagtag
     2221 cgcatacgct cgcgaatgtg agaaacttgt cgccggaaga attcgccgcc tccacggcag
     2281 caaattttcg gcgcttcttc catcttgtcc ggccgtcaga atctccggcc gcaacatttt
     2341 gatgcagcct gagtccggtc acatgattcg agcggtgcca ggaggaaccc catcgcgctg
     2401 ctaaccgcta aggagatctt cgatctagtc cgcgacgatc tcgtcctcgt cgaggaagaa
     2461 cttgcacggc aaagtgccac cgcatttgaa cccgtttcag aaattaccag ctaccttctg
     2521 ggtggcggcg gcaaacgcct gcgccccgcg ctgctcattc tgtgcaaccg ctatgcgggc
     2581 gcaaacagtc ctggtgcgat ccgcctggcg gccgtggtgg aactcctcca cagcgccaca
     2641 ctgattcacg atgacgtaat cgacagcgca aacacgcgtc gcggccgtcc ttccgcgaat
     2701 tccaggtggg gcaatcaccg cagcgttttg gctggcgatt ggttgtatat gcagtccttt
     2761 cagatggcgc tggaagaacg aaattttcga atcctcgatg tgctaatcga cctcacccaa
     2821 aaaatggtgg aaggcgaact cattcagctt gaaaaaatag gccgcattga cgtcacagaa
     2881 gacgacgccg tgcgtctcgc gacctacaaa accgcgtgtc tctttagcgg ctgcgcccgg
     2941 cttggcgcgg tgctagcggg cctcgaagga gaagaggaag aagctctcgc cgattacggc
     3001 cgctacgccg gtctagcctt tcaactcgtc gatgatctgc tggattttac cgcttcggca
     3061 aagcaactcg gaaaacccgt cctcagcgat ctgaaagaag gcaaagtcac tctgccgctc
     3121 atctacgcaa tggaaaatgg gcaccgcgac gctcgccaac tcgtcgccca ggttctcgaa
     3181 gaaaaagaat ttaattccgt ccgtccagaa acgctggtcg cgctggttca cgactccgga
     3241 gcactcggcc gcgcgcgcaa actcgctcat gattacgccc ggcgcgcgaa ggcttgtttg
     3301 aacgggcatt ccgacagcga atacggtcgc gcccttatga ccctcccgga ttttattctt
     3361 gcgcgagaga attgatcgct gtgtgtcgaa atgcttcctt ttaaggctgt ttaccatccc
     3421 cgttatgatt taaatcttgg cgcacatgtt tttccttcgc aaaaattccg tttagtctca
     3481 gaaagtctag ttagctccgg gatcgccgac gccagtgatt tcttgcgccc ggagccggct
     3541 tctgacgctg atctattacg cgtacacaca gcgtcctgga ttcgcaaact aagaacagga
     3601 acgctgaccg ccagcgaaat tatgaagctg gaggtcccct attcgaagga actggcggag
     3661 gccgtttggc tggcggcggg cggcacaatt ctggcggccc aatcggcgtt gcgcgatggc
     3721 tttggttcca atttgagcgg cggctttcat catgcctacg ccaatcacgg cgaaggattt
     3781 tgcgccattc acgatgtggc tgttgctgtc cgccggctcc aggcggatgg agcaatcaag
     3841 aaagccataa tcgtggatac cgatgtgcat catggaaatg gcacggccgc tattttcagc
     3901 ggggactcct cagt
//
LOCUS       DATCBB010000272         1992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_365247, whole genome shotgun sequence.
ACCESSION   DATCBB010000272 DATCBB010000000
VERSION     DATCBB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365247"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1163)
                     /locus_tag="VII37_03085"
     CDS             complement(57..1163)
                     /locus_tag="VII37_03085"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEY5068737.1"
                     /translation="MRFVTASCFIVLLLGGQCLAPAQPGKPDQLSLTQDQASHFAQLA
                     LKCVQKEYPNKPDHVINDPDDVRTPRAMHGAFYGCFDWHSSVHGHWMLVHLLRLFPAM
                     PEARAIRTALDANLSVENIRGENAYLLQQNRASFERTYGWAWLLKLAEELHGWHDEDG
                     QRWSQNLQPLADAFAEKYLSFLPKQTYPIRTGVHPNTAFGLAFALDYARAVGNQKLAT
                     LIVERSRAYYAHDMNYPAAWEPGGEDFFSPALIEADLMRRVMSETEFAGWFHRFLPDV
                     ARGGPKALIQPAIVTDRTDPKLVHLDGLNLSRAWCMRSIAVALPQNDPARRVLARSAL
                     EHAKVTLPHVASGNYEGEHWLASFAVYLLTTPSP"
     gene            1248..1976
                     /locus_tag="VII37_03090"
     CDS             1248..1976
                     /locus_tag="VII37_03090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5068738.1"
                     /translation="MKFSVIIPAYNEEEYLPRLLDSIEVARSNYSGGAAAIEVIVADN
                     DSTDRTAEVAAARGAQVVTVKKRRIAAARNGGGHAARGEIVCFIDADSALHPQTFNAI
                     DQAMKTDRYVWGVTGFVLERKSFALMLSYFLCIPLVLLTGLDSGLSFCRREDFEAVGG
                     YDESRLYAEDVKFPLALRRLGRSRGQRLTRLPKVKALGCTRKFDQFGDWHYFGMLGHV
                     LKSLVTGNWNNEQLAERYWYNSGR"
BASE COUNT          438 a          554 c          578 g          422 t
ORIGIN      
        1 tcttccccag cccttctccc agagggagaa gggagatgga aaatagttcg acgcgcttaa
       61 ggcgacggcg tcgtcagcag atacacagcg aaagacgcca gccagtgttc gccctcatag
      121 ttgccgctcg ccacatgagg cagcgtgacc ttcgcgtgct ctaacgcgga ccgggccagc
      181 actctgcgtg ccggatcatt ttgcggcaac gccacggcga tgctgcgcat gcaccaggcg
      241 cgacttagat tcaagccgtc gagatgaacc aacttgggat cggtgcggtc agtcacgatt
      301 gccggctgta tcaaagcctt tggtccgccg cgcgcaacgt ccggcagaaa gcgatgaaac
      361 caaccagcaa attctgtctc gctcataacg cggcgcatca ggtcggcctc aatcaacgcc
      421 ggtgaaaaga aatcttcgcc acccggctcc caggccgccg gataattcat gtcatgggca
      481 tagtaggcgc gactgcgctc gacgatcagc gttgccaact tctggttccc gacagcccgc
      541 gcatagtcga gcgcgaaagc cagaccgaac gccgtgttcg gatggacgcc ggtgcgaatc
      601 ggataggttt gtttgggaag aaagctgaga tacttctcag caaacgcatc cgcgagcggc
      661 tgaagatttt gcgaccagcg ctgtccatct tcatcatgcc agccgtgcag ttcctcagcg
      721 agtttcagca accaggccca accgtaagtg cgttcgaatg aagcgcgatt ttgctgaagc
      781 aaataagcgt tttcgccgcg gatgttctca acactcagat ttgcgtcgag cgcggtccga
      841 attgcgcgcg cttcaggcat cgccgggaat aggcgcagga gatgaaccag catccagtga
      901 ccgtgcaccg acgagtgcca atcaaaacaa ccgtagaacg ccccgtgcat cgcccgcggg
      961 gtgcgcacgt cgtccggatc attgatcacg tgatcgggtt tgttgggata ttctttttgg
     1021 acgcacttca acgccaattg cgcaaaatgt gaagcttggt cctgcgtgag cgaaagctga
     1081 tcgggctttc ccggctgtgc cggcgcaagg cattgaccgc caagaagcaa gacaatgaaa
     1141 caagatgctg ttacgaagcg catgcgttcc ctccggatag tcttcttcca ccgtttggat
     1201 caaggaaggt tttccaagtc taacagaatc tgtacactgt aagagagatg aaattctcag
     1261 taatcattcc cgcttacaac gaagaggagt acctgccgcg tctgctggat tccatcgaag
     1321 tggcgaggtc gaattattcc ggcggtgcgg ctgcgattga agtcatcgta gccgataacg
     1381 attccaccga ccgcactgcc gaagtggcgg ccgcgcgcgg cgcgcaagtc gtcacggtta
     1441 agaagcgtcg catcgccgct gcccgcaacg gtggtggcca cgcggctcgc ggagagattg
     1501 tttgtttcat cgatgcggac tcagcgctgc atccgcagac cttcaacgca attgatcagg
     1561 caatgaaaac cgatcgctat gtttggggcg tcacgggttt tgtattggaa cgaaaatcct
     1621 tcgccttgat gcttagctat ttcctgtgta ttccgttggt tcttctaacc ggactcgact
     1681 ccggcctgtc gttttgccgg cgcgaagact ttgaagcggt cggcggttat gacgagtcac
     1741 ggctgtacgc cgaggacgta aagtttccgc tggcgttgcg tcgccttggt cgttcgcgtg
     1801 gacagcgact aacgcgctta ccgaaagtaa aagcgctcgg ctgtacgcgc aagttcgatc
     1861 agtttggcga ctggcattat ttcgggatgc tgggccacgt gctcaagagt ctggtcacgg
     1921 ggaactggaa caacgagcaa ctggctgagc gctactggta caactctggc cggtaggcta
     1981 ggcgactgca ga
//
LOCUS       DATCBB010000273         1153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_170968, whole genome shotgun sequence.
ACCESSION   DATCBB010000273 DATCBB010000000
VERSION     DATCBB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170968"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VII37_03095"
     CDS             <1..282
                     /locus_tag="VII37_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068739.1"
                     /translation="KNPLTYEIISPETVGVPSRLMVLGKHSGRNALRMSLRDLGYEPS
                     EAELAEVYQRVNALADKSKSVRPRDLVAIAHEVLRRRPAAVAAEASSTA"
     gene            346..>1153
                     /gene="leuB"
                     /locus_tag="VII37_03100"
     CDS             346..>1153
                     /gene="leuB"
                     /locus_tag="VII37_03100"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY5068740.1"
                     /translation="MKKNIALLPGDGIGPEVTRAVAAVLRECAHEFSHQFDMHEFPIG
                     GAAIDATGTPLPAETLDACRKADAVFLGAVGGAKWDSLPVGKRPESGLLELRKGMGLY
                     VNVRPVKVLEPLRGISPLKPERLGDLDIEIVRELAGGMYFGELGSVTEKGEERAWDTE
                     SYSTREIERIATFAFSRAESRSRRLCSVDKANVLSSSQLWRRTVTAMSTVYRYVKLEH
                     MYVDNAAMQLTLKPTQFDVILSSNIFGDILSDAAAALVGSIGLVPSASFGS"
BASE COUNT          251 a          275 c          386 g          241 t
ORIGIN      
        1 aagaatccgc tgacgtacga aattatttcg ccggaaacgg tgggcgtgcc ttctcgtctg
       61 atggtgcttg ggaagcattc tggacgaaat gctttgcgca tgagcttgcg cgatttagga
      121 tatgagccaa gcgaagcgga gctggcggaa gtttatcagc gggtgaacgc gctggcggac
      181 aaatcgaaga gtgtgaggcc acgcgacctg gtagcgattg cgcacgaagt tttgcgacgg
      241 cgtccagcag cggtggctgc ggaagcatca agcacggctt aaggataaat tgatttggcg
      301 ctacgtctgg caccacatga gaaagcagca ggaccggaat tttcgatgaa gaaaaacatc
      361 gcacttttac cgggagatgg cattggtccg gaagtaacca gggccgtggc tgcggttttg
      421 cgcgaatgcg cgcacgaatt ctcgcatcaa ttcgacatgc acgaatttcc gatcggcggc
      481 gcggcgattg acgctaccgg tacgccgctg ccggcggaga cattggatgc atgccgtaaa
      541 gcggatgcgg tttttcttgg cgccgtgggc ggagcaaagt gggattcact tccggtaggg
      601 aagaggcctg agagcgggct gctggaattg cgcaaaggaa tgggcctgta tgtgaacgtg
      661 cggcccgtca aagtgctgga gccgctgcgc ggaatttctc ctttgaagcc ggagcgcctc
      721 ggcgatctgg atattgagat tgtgcgcgaa ctggccggag ggatgtactt cggcgagctc
      781 ggcagcgtta cggaaaaagg cgaggagcgc gcgtgggata cggagtcgta ttcaacgcga
      841 gaaattgagc gcatcgccac attcgcgttt tcgcgcgcgg agagccgttc gcggcgactg
      901 tgctcggtgg acaaagccaa cgtgctgagc agttcgcagc tatggcgccg cacggttacg
      961 gccatgagca cggtatatcg ctacgtgaag ctcgaacaca tgtacgtgga taacgcggcg
     1021 atgcagttga cgctgaagcc cacgcaattc gatgtgattc tcagctctaa catatttggc
     1081 gacattttaa gtgatgcggc cgcggcgctg gtggggtcga tcggacttgt gccttcagcc
     1141 agctttggat cag
//
LOCUS       DATCBB010000274         1073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_203311, whole genome shotgun sequence.
ACCESSION   DATCBB010000274 DATCBB010000000
VERSION     DATCBB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203311"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..261)
                     /locus_tag="VII37_03105"
     CDS             complement(133..261)
                     /locus_tag="VII37_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068741.1"
                     /translation="MTLLKKRLAEARTDAERDVILKLLADEEAKESPRRNGILQSR"
     gene            783..>1073
                     /locus_tag="VII37_03110"
     CDS             783..>1073
                     /locus_tag="VII37_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068742.1"
                     /translation="MARLFAALTRGLPQPVAILISSGPDWTDRTKRLAARAPDLDIFS
                     QSFVLRDAAGWQISDAGRALLASIEAPLPTKAEIEPAPPAAVVSHVPIQSSPP"
BASE COUNT          221 a          320 c          293 g          239 t
ORIGIN      
        1 tgagcggggc gtttacactg agcttcgtat gggggtccgg ggggcatccg cccaatgcaa
       61 tcagaattgg agttctggct tgagtgcgct agcgtattca atacgtccag cgcggcggct
      121 ccttgatgca gatcagcgag actggagaat gccatttctc cgcggagact ctttcgcctc
      181 ctcatccgcc agcaatttca agattacgtc gcgctccgcg tcggtgcgcg cttccgcgag
      241 acgtttcttc aacagggtca agttctccct gtgaatgtac ttctccattt ccatagctga
      301 tctccctgat tagagcgaaa agccatcttt ggggcgagaa acgcgcgcag cccatgcgcg
      361 caacgcctcc gacttcaggg ggtcttccga acttcccacc gtccggacat tgtgctgcgc
      421 cttcgggcat ccatcgcacc cggggaaatc aaacacgcag aactcataac ttgaaggcgc
      481 atctgccgcc gtcatttcga agcggcacct caacttggta ggccgaactt ggttttgctg
      541 attttggggc atgatgctcc ccccagatca ggcggaagct caacactccc agtcaccggt
      601 ggatgcccag atcggagcgg tgaacctcga aaatcctagg gtcgcttaat tgagggggcc
      661 agagaccatc ggaagtgaaa agttgatcaa gatcaaattt ctggttgatt tttggtggca
      721 tattgtttgt ttgctgcgat ctgaggcgaa aatggcgagg gcgaaaatcc caactaacgc
      781 tcatggcccg gctattcgcg gcgttgaccc gtgggctccc tcagccggtc gcaatcctga
      841 tttctagcgg cccagactgg accgatcgaa caaaacgctt ggccgcgcgc gcgcctgatc
      901 tcgatatctt cagccaatcg ttcgttctgc gagacgctgc tggctggcag atctccgatg
      961 ccggccgagc gctactcgct tccattgaag cgccattgcc cacgaaagcc gaaatcgaac
     1021 cggcgccgcc agccgcagtc gtaagccacg tgcctatcca atcatctccg ccg
//
LOCUS       DATCBB010000275         1267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_300889, whole genome shotgun sequence.
ACCESSION   DATCBB010000275 DATCBB010000000
VERSION     DATCBB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300889"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VII37_03115"
     CDS             complement(<1..493)
                     /locus_tag="VII37_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5068743.1"
                     /translation="MNWEAFWLTVRLAAIVSALLIVIGIPIAYWLTYSRRRWKFLIEA
                     VVALPIVLPPTVLGFYVLVALGSRSPLGRWWESLTGHTLAFTFEGLVLGSLLYSLPFA
                     VQPFAASFGAVDPKLLRASASLGDSPLQTFRRIVLPLSKAGLVTGFALSFAHTLGEFG
                     VVLM"
     gene            complement(677..>1267)
                     /locus_tag="VII37_03120"
     CDS             complement(677..>1267)
                     /locus_tag="VII37_03120"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5068744.1"
                     /translation="GPARIVPLEYKEEEADEKQSYPLDTARLRRVVEMAAEKSGWAKR
                     SKDMGKGRGMGIAAHRSFLTYVATVVEVEVDGRGQVHIPGVWTALDAGTVVSPDNVRN
                     QFEGAATFGTSLALFGEITATNGMIDQSNFNNYQMARMNRAPRHVDVQIVESDAPPAG
                     VGEPGVPPFAPALFNAVFAATGKRVRELPLSKTKLV"
BASE COUNT          288 a          401 c          331 g          247 t
ORIGIN      
        1 ccatgagcac aactccaaat tctccgagcg tgtgtgcaaa gctaagcgca aaccctgtga
       61 ccaatcccgc tttcgagagg ggaagcacaa tgcggcgaaa agtctgcaga ggcgaatcgc
      121 caagcgaggc tgacgctcga agcagtttcg gatcgaccgc tccaaatgaa gccgcaaacg
      181 gctggaccgc gaatggcaaa ctgtatagca atgacccaag tacgagccct tcgaatgtaa
      241 acgccagcgt gtggccggtg agcgactccc accagcggcc gaggggacta cgggacccga
      301 gtgccaccaa tacgtaaaat ccaagcacgg tcggcggcaa cacgatcgga agcgccacca
      361 cggcttcgat cagaaatttc cagcgcctgc gagagtaagt cagccagtac gcgatgggaa
      421 ttccgataac aataagtagc gcggagacga tggccgccag acgtactgtc agccagaacg
      481 cctcccagtt cattaacgct cctccgttcg aatcgctctc cctgacaagt tgaaaccgta
      541 attagcgggc agggaagtgg cttcaggggt acgcaaatat tccaggaatt cacgcgtcgc
      601 acatcggttt gttgaacgca atatgccccg tttcagatgg ggcaatctcg tcccttcagg
      661 cggattattg cgcaggctac accagcttcg tcttcgacag cggcaactcg cgcacccgct
      721 ttccagtagc cgcgaacacc gcgttaaaca aagcaggcgc gaaaggcggc acgcccggct
      781 cgcctactcc cgccggcggt gcgtcgctct ccactatttg cacgtcaaca tgccgcggcg
      841 cgcggttcat acgcgccatc tgataattat tgaaattgct ttggtcgatc attccattcg
      901 tcgcggtaat ttctccgaac aatgccaggc tagttccaaa cgtcgccgcg ccttcgaact
      961 ggttacgaac gttgtccgga ctgacgaccg ttcccgcgtc gagtgccgtc caaacccctg
     1021 gaatatgaac ttggccgcgc ccatccacct caacttccac cacggttgcg acataagtaa
     1081 ggaagctgcg atgggcagca atgcccatcc cgcgcccttt acccatgtct tttgatcgct
     1141 tggcccagcc cgatttctcc gccgccattt cgaccacgcg ccgcagccgc gctgtatcga
     1201 gcgggtagct ctgcttctcg tcggcttcct cttctttgta ctcgagcgga acaatacgcg
     1261 ccggccc
//
LOCUS       DATCBB010000276         5799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_138696, whole genome shotgun sequence.
ACCESSION   DATCBB010000276 DATCBB010000000
VERSION     DATCBB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138696"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VII37_03125"
     CDS             <1..289
                     /locus_tag="VII37_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5068745.1"
                     /translation="RNSLEVIIEDWCIGCGLCARNCPYGNINLHPFHVMNEDPDHPGR
                     TKAVVKQKATSCDLCTNLAEPSCVYACPHDAAHRVDPKTFFADAIGQSSSY"
     gene            307..1254
                     /locus_tag="VII37_03130"
     CDS             307..1254
                     /locus_tag="VII37_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068746.1"
                     /translation="MRIDSTQRTWAIASAIIFAASLIAYIVYAQNSIQGARGGSTVGL
                     TFGILGFAFMLFAALLGARKHVPVWRLGRAQAWMRGHLWLGALSLPIILFHGGFHFGG
                     TLTRVLMWLLIFTVFSGLFGAVLQHYIPRVMTADVPLETIYDEIGTVRNLLLEEADRA
                     MEGLCGAIGGGKPSLEEVQRAGGFTAMRPMAAAARGGTAVAPAAEILLLSEEESAPLR
                     RFYFNEVRPFLERPAERRLLLSDAGKAQLGFSGLRTLLPERAHATVDDLEDVCEEARQ
                     LQKQRRLHHWLHLWLLVHIPISLALIVLGAVHAVMALKY"
     gene            1316..2731
                     /locus_tag="VII37_03135"
     CDS             1316..2731
                     /locus_tag="VII37_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068747.1"
                     /translation="MARTRTTKKLAQRIDLNYFKRPTPLKRAKFWLSILLPILAIMWI
                     SWYGFHRDARVYSSGRMSAPHAILEKQCAACHVRKASVFSAKVQNQACLSCHDGPIHH
                     INQASTPDCATCHTEHRGRINIAATSEKSCTECHANLRTTGAPAIYSQHIRNLADGHP
                     EFAVIRENRRDPSTIKLNHALHMKLIRRGPTGPIVQLNCADCHRPAAAKVSWAYADAN
                     YATGSTTRETPNTNTSNGDVLAAHNPPSGRELMAPVTFANACASCHLLSFDKRFSEGV
                     PHDKPEVIHAFLKKNFQDYVAAHPSELRSGRDPDRDITGRPLAPRTQILTAAQWVSVR
                     TADAEQLLWRKTCLQCHQLSFNRNSKLPVVQQTAAASEDALPQIAEANVTARWMPHAK
                     FDHEAHGGFTCVTCHEKALRSSESSDVLLPGIKACQTCHAPGPDHAESRCFECHTYHD
                     WSKRKEITPTFSFPPLKTGGL"
     gene            2862..5111
                     /locus_tag="VII37_03140"
     CDS             2862..5111
                     /locus_tag="VII37_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY5068748.1"
                     /translation="MNAGSGVPSESRPSAALAFWQILTLFDSTKLSTHRAFRNALGVV
                     LPLIAGFALHMPRGGLVVASGALNVSYSDGSDPYSARVKRMLASSVICAVAVVVGAVS
                     GKHVVLAVTLAGIWAFIAGMFVAVGGAAPDLGVISLVTLLIYAAQHLAPREAAISGLL
                     ALAGGLLQTGLSVALWPVRRYDPERRALEKFYLELANRAAAAWTATSAPLGSAQSEQA
                     QEALLGLARDGSTESIRYLALLNQAERMRLALLMLMRLRVRMQREIPNYPGIAILKGY
                     LEAVSEILMHISNSLSAATLAEPTEKAGPAVEALDRFSIRMREAAEATPASFIAAVAK
                     DAVFQMDALSGQLRAALDLSQNNVELQGLAEAPSVTPKTWKEAVSATIEAFRNHLNLR
                     SSIFRHALRLSVLVALGDILGTEVSWRRTYWLPMTIALVLKPEFTATFTRGLLRIAGT
                     IVGLLLATGVFHLFHPGVVLQVILIFVFVFLMRWLGPANYGIFGIAVSALIVLLLAIA
                     GVPPKDVIWARGVNTVVGGSLALLAYWLWPTWERTRVSERIAEMLDAYRNYAHALSRW
                     NPADESWMQELERARRGARSARANLEASIDRLSSEPGTTREQLARLSATLASSHRLIH
                     ALMALDAVFSHNPALASSAPFRNYISKLDKTLTLLAATLRGVRVASKDIPNLREEHRL
                     MVQARTIEENSVLAPFDSINIEIDRITNSVNTLAEQIMQWTRTPEFAALHKQNLELET
                     GQANIQSDS"
     gene            complement(5189..>5799)
                     /locus_tag="VII37_03145"
     CDS             complement(5189..>5799)
                     /locus_tag="VII37_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068749.1"
                     /translation="AIAGQQPAMFENKIQTRMDEILKRATQEFESAATRVSDRHLIRL
                     MEDKQMVAREATAQMEARAAEARAVLHSAAGDAVDEFRRQLQSQVDMMIAEASQKATS
                     ALASIEAESRSACETRRRSLETDVSRVAEQSAEQFRTGIKAFLYSCLVAAVSAVDEHS
                     QSTLEGLVKDGPKDAKENGHNGKLPHQFTIPGDGSNENNNEN"
BASE COUNT         1222 a         1697 c         1563 g         1317 t
ORIGIN      
        1 acgcaattcg cttgaagtca tcattgagga ctggtgcatt ggctgcggcc tgtgcgcacg
       61 caattgccct tacggcaata tcaatttgca tcctttccat gtgatgaacg aagatccaga
      121 tcatcccggc cgcaccaagg cagtcgtaaa acaaaaagcc acttcctgcg acctgtgcac
      181 caacctggcg gagcccagtt gtgtttacgc atgcccgcac gacgcagctc atcgcgtcga
      241 tccaaaaacc ttctttgccg atgccatcgg ccagtcgtcg agctactaag acggagcgct
      301 tgagcgatgc gcattgacag cacccagcgt acttgggcca tcgcttccgc aataattttt
      361 gccgcttcgc tgatcgccta tattgtttac gctcagaatt ccattcaggg cgcccgaggc
      421 ggcagcactg tcggcctcac gttcggtatt ctcggtttcg cgttcatgct ttttgcggcg
      481 ctgcttggcg cacggaagca cgtgccggta tggagactcg ggcgcgctca agcatggatg
      541 cgcggccatc tctggctagg cgcactcagt ttgccgatta ttcttttcca tggcggattt
      601 cactttggcg gcacgctcac ccgcgtgctg atgtggctgc ttattttcac tgtgttcagc
      661 gggctttttg gcgcggtgct gcagcactac attccacgcg ttatgactgc cgatgtgccg
      721 ctggaaacca tttacgatga gattgggact gttcgcaacc tgcttctcga ggaagccgat
      781 cgcgccatgg aaggattatg cggcgcgatc ggcggcggca aaccctcgct tgaggaagtc
      841 cagcgcgccg ggggattcac agctatgcgt ccaatggccg ctgccgcaag aggaggcacc
      901 gctgtcgcgc cagccgccga aattttattg ctgagcgaag aagaaagcgc cccgcttcgg
      961 cgtttctatt tcaatgaggt gcgccctttt ctggagcggc ccgcggaacg ccgattgttg
     1021 ctttctgacg ccggcaaagc gcagctcgga ttttcgggtt tgcgcacgct gctcccagag
     1081 cgtgctcatg caacagtgga cgacctggag gacgtgtgcg aagaagcgcg gcaacttcag
     1141 aaacagcgcc gtctgcacca ctggcttcat ctgtggctac tggtgcacat tcccatttcg
     1201 ttggccctga ttgtgttagg tgccgtgcac gcggttatgg cactgaaata ttgagtctga
     1261 tttttgaaac agcgcgcagg aaaccggcgc gctgtgtgac cggcggttaa aaaacgtggc
     1321 tagaacgcgc accaccaaaa aactcgccca gcgcatcgat ctcaactact ttaagagacc
     1381 gactccgctg aaacgcgcca agttctggct gagtattctt cttcctatct tggcgattat
     1441 gtggatttcc tggtacgggt ttcacagaga tgcgcgcgtg tacagcagtg gccgcatgtc
     1501 cgcgccacac gccatcctgg aaaaacaatg tgcggcgtgc catgtgcgga aggcgtcggt
     1561 attctcagcc aaggttcaaa atcaagcttg tctttcgtgc catgacggcc cgatccacca
     1621 cattaatcaa gcgagcactc cggactgcgc cacctgccac actgaacatc gcggccgcat
     1681 aaacattgcc gccaccagcg aaaaatcctg caccgaatgc cacgccaatc tgcgaaccac
     1741 cggcgcgccc gcgatttact cgcagcacat tcgcaatttg gccgacggtc acccggaatt
     1801 cgccgttatc cgcgaaaatc ggcgcgatcc ctcgacgatc aaattgaacc acgcgctcca
     1861 tatgaaactc attcgccgcg gcccaaccgg tccgatcgtg caattgaatt gcgccgattg
     1921 ccatcgcccg gctgcggcaa aggtttcatg ggcgtatgcc gacgcgaatt atgcgacagg
     1981 ctcaaccacg cgcgaaacgc caaacacgaa cacgtcgaat ggagacgtat tagcggctca
     2041 caatccgccg agcggccgcg aattgatggc tcctgtgact tttgccaatg cctgcgcgtc
     2101 ctgtcatctc ctttcattcg acaagcgctt ctccgaaggt gttccgcacg acaagccaga
     2161 agtcattcat gcttttctga agaaaaactt ccaggattac gttgcggcgc atccatccga
     2221 actgcggtcg gggcgtgatc cggaccggga tataacggga agacctctcg cccctcgaac
     2281 acaaattcta accgctgcgc agtgggtttc cgttcgaacc gctgacgccg aacagcttct
     2341 ctggcgcaag acttgtttgc aatgccatca gctttccttc aatcgaaata gcaaattgcc
     2401 ggttgtgcag cagacggcgg cagcgagcga agacgctttg ccacaaatcg ctgaagccaa
     2461 tgtcaccgca cgttggatgc cgcacgccaa gtttgaccac gaggcccacg gaggttttac
     2521 ctgtgtgact tgccacgaaa aagccttgcg cagctcggag tccagtgatg tgctattgcc
     2581 cggaattaaa gcctgccaaa cctgtcatgc tcctggcccg gaccacgccg aatcgcgctg
     2641 cttcgaatgc cacacgtatc acgactggtc aaagagaaaa gaaattacgc ccaccttcag
     2701 ttttcccccg ctaaaaacag gtgggctgtg attcgcccga aacagaaatt ttaatcatct
     2761 aatcgtctcg aaagaacggc gcagggtgga tttagctaca attgtttttc gacatgtcct
     2821 cagtatcaag cgaagtctca gaccaatcct cgaaagaagt cttgaacgct ggctccggcg
     2881 tcccttctga atcacgccca agtgccgcgc tggcgttctg gcaaattctc acactttttg
     2941 attccacgaa gctttccacg catcgagcat ttcgcaatgc cctcggagtg gtgcttcctt
     3001 taatcgcagg cttcgccctg cacatgccgc gcgggggact cgtcgtggcc agcggcgccc
     3061 taaacgtgtc ctactccgac ggcagtgatc cgtactccgc gcgcgtcaag cgcatgttgg
     3121 cctccagcgt catctgtgcg gttgcggttg ttgtcggagc cgtttccggc aagcacgtag
     3181 ttctcgcggt cacgctggct ggaatttggg ccttcatcgc cgggatgttt gtcgcggttg
     3241 ggggagccgc cccagacctg ggcgtcatca gcctggtcac attgctgatc tatgccgccc
     3301 agcatttagc gccgcgagaa gccgccatct ccggactcct ggctctggcg ggaggtttgt
     3361 tgcagacagg attgtccgtc gcactttggc cggtgcgccg ctatgatcct gaacgccgcg
     3421 ccctggagaa gttttatcta gagttagcaa atcgtgctgc tgcggcgtgg accgccactt
     3481 ctgctcctct gggaagcgct caaagcgagc aagcccagga agcattgctt gggctcgcgc
     3541 gcgatggcag cactgagagc atccgttatc tcgcgctgct caatcaggct gagcgcatgc
     3601 gactcgcact gcttatgctc atgcgcttgc gagtacgcat gcaaagagaa attccaaact
     3661 acccgggaat tgccatcctc aagggttatc tcgaagcagt ctctgaaatt ctcatgcaca
     3721 tcagcaactc gttgtccgct gcgactcttg ccgaacctac agaaaaagcc ggcccggcag
     3781 ttgaggcgct cgaccggttt tccattcgga tgcgtgaggc agccgaagca actcccgcgt
     3841 cattcatcgc cgccgtcgcc aaagacgcgg tattccagat ggacgcgctg agcggacaac
     3901 tgcgcgccgc actggacctg agtcaaaaca atgttgaact acagggattg gccgaggctc
     3961 catctgtcac accaaaaacc tggaaggaag ctgtctcggc aaccatagaa gcatttcgca
     4021 atcacttgaa tttgcgatct tccatctttc ggcacgctct ccgtctctcg gttctggtcg
     4081 cgctgggaga cattctaggc accgaggtct cctggcgccg cacctattgg cttccgatga
     4141 ccatcgcgtt agtgctcaaa ccggagttta cagcgacgtt tacgcgggga ttgttacgta
     4201 tcgctggaac aattgttgga ctcctgttgg ccaccggggt ctttcatctg tttcatcctg
     4261 gagtcgtgtt gcaggtcatc ttgatttttg tgtttgtttt tctgatgcgc tggctcggtc
     4321 ccgccaatta cggcattttc ggaatcgcgg tgagcgcttt gatcgtgctt ctactggcta
     4381 tcgcgggggt gccgccaaaa gatgtgattt gggcccgcgg cgtgaacacc gtagtcggtg
     4441 gctcgctggc gctcctcgcg tactggctgt ggcccacgtg ggaacgcacc cgggtgtccg
     4501 agcgcatagc ggaaatgttg gacgcataca gaaactatgc tcacgcgctg tcgcgatgga
     4561 acccagctga cgaatcctgg atgcaggaat tggaacgtgc gcgtcgcggc gcccgaagcg
     4621 cccgagcgaa cctcgaggcg tccatcgatc gtctaagctc ggagcctgga accacacgag
     4681 aacagctcgc caggttgagc gccactctag ccagttcaca ccgactcatt catgccctca
     4741 tggcgctgga tgcagttttt tctcacaacc ccgcgcttgc tagttctgcg ccttttagaa
     4801 attacatttc caaattagac aagacgctga cgttgctcgc tgccactctt cgtggagtac
     4861 gggtagcgtc gaaagacatc cccaacctgc gtgaagaaca ccgcttgatg gttcaggcta
     4921 gaacaatcga ggaaaactcc gtgctggcgc ccttcgattc gataaatata gaaattgacc
     4981 gcatcaccaa cagcgtgaac actctggcag aacaaatcat gcaatggaca cgcacaccgg
     5041 aatttgcagc attgcataaa caaaacttgg aattggagac gggacaagca aatatccagt
     5101 cagactcata ataacggcga ataagcgacg cggagtcaac gtgacttgaa cagaagccaa
     5161 gcggccaagt taccaaggca attggtaatt aattttcgtt gttgttttcg ttggaaccgt
     5221 cgcccgggat tgtaaattgg tggggcaatt tcccgttgtg cccgttttcc ttggcatctt
     5281 ttggcccgtc cttgacgagg ccttcgaggg tgctctgtga gtgttcgtcc acggcgctaa
     5341 ccgcagcgac caggcacgaa tatagaaatg ccttaatgcc agtgcggaac tgctctgcgg
     5401 attgttccgc gacacgcgac acgtctgttt cgagcgagcg ccggcgcgtc tcacacgcgg
     5461 aacgactctc ggcttcaatc gaagccaacg ccgaagtcgc tttctgtgaa gcctcggcga
     5521 tcatcatatc gacttgtgac tgaagttggc gacggaattc atcgaccgcg tcgccggcag
     5581 cgctatgcag aaccgcacgg gcttccgcag cccgcgcttc catttgagcg gtagcttctc
     5641 tcgccaccat ttgcttatct tccatcagtc gtatcaggtg acggtccgaa acacgtgtcg
     5701 ccgcagattc gaactcctgg gtggcgcgct tcaggatttc gtccatgcgc gtctgaattt
     5761 tgttctcaaa catcgccggt tgctggcccg cgattgcaa
//
LOCUS       DATCBB010000277         1034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_300912, whole genome shotgun sequence.
ACCESSION   DATCBB010000277 DATCBB010000000
VERSION     DATCBB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300912"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VII37_03150"
     CDS             <1..256
                     /locus_tag="VII37_03150"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5068750.1"
                     /translation="IPCREGTTWLRKMLERIHAGFGREQDIDMLSELSKNILGRSFCP
                     LGDAAAMPTISIVQKWRGEFEEHLRGGCAYKSAEALAGSR"
     gene            275..>1034
                     /locus_tag="VII37_03155"
     CDS             275..>1034
                     /locus_tag="VII37_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5068751.1"
                     /translation="MADQKLITLKINDRDVQVAPGTLVIEATRRIGTEVPSFCYYPGL
                     SLQAACRMCLVEVEKMPKLQTACTLVATEGMIVRTNTQQVRDARKYMLEFLLTNHPLD
                     CPVCDKGGECELQDMVFRYGADTSRFIEEKIHRPEEKWSELVYYDAPRCILCFRCVRV
                     CDEGMDVKALGVGMRGANSVIIPNRGDHLECEECGMCIDICPVGALTSGTYRYKTRPW
                     EMQYAPTICTHCSNGCKTTLSVRNHEIMRSNNRDL"
BASE COUNT          229 a          266 c          352 g          187 t
ORIGIN      
        1 cattccgtgc cgtgaaggca cgacgtggct gcgcaagatg ctggagcgga tccacgcggg
       61 attcgggcgc gagcaggaca tcgacatgct cagcgagctt tccaaaaata ttctgggacg
      121 gagtttttgt ccgctgggag acgcggcggc gatgccgacg atcagcatcg tgcaaaagtg
      181 gcggggcgag tttgaagaac atttgcgcgg gggctgcgcg tacaaatccg cggaagcgct
      241 ggccggcagt cgatgaagcg aatgaagcga atccatggct gaccaaaaac tgatcacact
      301 gaaaattaac gaccgcgacg tgcaggtggc gccgggaacg ctggtgatcg aggccacgcg
      361 gcgcatcggc acggaagtgc cgtcgttctg ctattacccg ggcttgtcgc tgcaggcggc
      421 gtgccgcatg tgcctggtgg aagtggaaaa aatgcccaag ctgcagacgg cgtgcacgct
      481 ggtggctacc gaaggcatga tcgtgcgcac caatacgcag caggtgcgtg atgcgcggaa
      541 gtacatgctg gaatttttgc tgaccaatca tccgctggac tgcccggtgt gcgacaaggg
      601 cggcgagtgc gaactgcagg acatggtttt ccgctacggc gcggacacca gccggtttat
      661 cgaagaaaaa attcaccggc cggaagaaaa atggtcggag ctggtgtact acgatgcgcc
      721 gcggtgcatt ttgtgctttc gctgcgtgag agtgtgcgat gaaggcatgg acgtgaaggc
      781 gctgggcgtg gggatgcgcg gggccaacag cgtgatcatt ccgaatcgcg gcgaccacct
      841 ggagtgcgaa gagtgcggga tgtgcatcga tatttgcccg gtgggcgcgc tgaccagcgg
      901 aacgtatcgc tacaagacgc ggccgtggga gatgcagtac gcgccgacga tttgcacgca
      961 ctgctcgaat ggctgcaaaa ccaccttgag cgtgcgcaat cacgaaatca tgcgctcaaa
     1021 caatcgcgac cttt
//
LOCUS       DATCBB010000278         1378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_268442, whole genome shotgun sequence.
ACCESSION   DATCBB010000278 DATCBB010000000
VERSION     DATCBB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268442"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VII37_03160"
     CDS             <1..409
                     /locus_tag="VII37_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY5068752.1"
                     /translation="NALAKRLTAVYVFTPQLAFMLVVISVVTMAGIWAATRAEKLLQR
                     KDPQIVVIDEVAGQMIALLSGPFWFHTWWSIFTAFLLFRAFDIWKPYPIRRLEGLESG
                     LGIMLDDVAAGAYALIVNSVLISGYLLIFPSSR"
     gene            479..>1378
                     /locus_tag="VII37_03165"
     CDS             479..>1378
                     /locus_tag="VII37_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY5068753.1"
                     /translation="MADDVSDSGLQPLREAGFVVEKKTGLALAALREALADSEGLVVR
                     SETKVTPDLMDAARKLRVIGRAGVGVDNIDVPAATARGIVVMNAPDGNTITTAEHTLA
                     LLVALARNIPQANASVKAAKWERKRFIGAELQGKTLGIIGLGRIGRAVAARARAFGMK
                     IVGHDPFIAPDQARDLEIESASMDEVFSRADFLTVHTPLTSETRGLIGAPAFAKMKKG
                     ARIINCARGGLVDESALYDAIKSGVVAGAALDVFEQEPPPPDHPLLGLDEVIVTPHLG
                     ASTTEAQEGVAFTVAEQMRDYLLT"
BASE COUNT          284 a          369 c          423 g          302 t
ORIGIN      
        1 caacgcttta gcgaagagac tgacggccgt gtacgtcttc acgccccaac tagctttcat
       61 gctggtcgtg atctctgtgg tgaccatggc cggcatttgg gcggcgactc gcgcagaaaa
      121 actgctgcaa agaaaagacc cgcaaatcgt cgtcatcgac gaagtcgcgg gccaaatgat
      181 tgcactgctg tctggaccgt tctggttcca cacctggtgg agcatcttca cggcgttttt
      241 attgtttcgc gcgttcgata tctggaagcc ttatcccatt cggcggctcg aaggtctgga
      301 atccgggctg ggcataatgc tcgacgatgt ggccgcggga gcatatgcgt tgattgtgaa
      361 ttcggttttg atttcaggat atttgctaat ctttccatcc agtcgctgat atccaatcac
      421 tgatcactta gaaaggctta catttcaaac ggtgccgatc cagcacataa agattttggt
      481 ggcagacgac gtgagtgaca gcggtttgca gccgctgcgc gaagccggtt ttgtggtgga
      541 aaagaaaacc gggctcgccc tcgccgcact acgcgaagcg ctggccgaca gcgagggcct
      601 ggtcgtgcgg agcgagacga aagtaactcc cgacctgatg gatgcagccc ggaagctgcg
      661 cgtaatcgga cgcgccggtg ttggcgtcga caacattgac gtgccagcgg cgacagcgcg
      721 cggcattgtg gtgatgaatg cgcccgatgg caacacgatt acgaccgcgg agcacactct
      781 cgctttgctg gtcgcgctcg cgcgcaacat accgcaggct aacgccagcg tgaaagccgc
      841 gaagtgggag cggaaacgtt ttattggagc tgagctgcaa ggaaagacgc tcggcattat
      901 tggcctgggc cgcattgggc gtgcggtggc ggcccgcgcc cgcgcgtttg gaatgaaaat
      961 cgtcgggcat gatcctttca tcgcgccgga tcaggcgcga gatcttgaaa tcgagtcggc
     1021 ttcaatggac gaagtgtttt cgcgggccga ctttcttacc gtccatacac ctttgacttc
     1081 ggaaacccga ggtctgattg gcgcgccggc gttcgccaaa atgaaaaagg gcgcgcgcat
     1141 tattaactgt gcccgcggag gattggttga cgagtctgcg ctctatgacg cgatcaagtc
     1201 aggagtagtc gccggcgctg cactggatgt tttcgaacag gagccgccgc cacccgatca
     1261 tcctttgctt ggtctggatg aggtgatcgt gacgccgcat ctcggggctt cgaccactga
     1321 agcgcaggaa ggcgtcgcct ttaccgtggc ggaacagatg cgcgattatc tgctgacc
//
LOCUS       DATCBB010000279         1275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_365390, whole genome shotgun sequence.
ACCESSION   DATCBB010000279 DATCBB010000000
VERSION     DATCBB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365390"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VII37_03170"
     CDS             <1..1061
                     /locus_tag="VII37_03170"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEY5068754.1"
                     /translation="RKCAAFELPIGPEDRFGDVDVQDICKRLAEAGFLHRAGENYHWT
                     QEAYPADTVSLRSVTSDNFVIIDITGAPSVIGEVDFSSALTTVHEKAIYIHGGQQYHV
                     EHLDFKERKAYVKRVDVEYYTDAIRYTQVRILECAAETRVNDSIPEIPALRSFGDVLV
                     RSQVIGFKKIKFFTNENIGAGNLELPENEMHTTSYWITLERPLLESLPFTISERQSGM
                     FGLLHALASIATLLLMCDRRDLGTAIGEKPPYPNDHDAGIEAVELRDRISQNAKAFYE
                     PNLYLFDAYPGGIGFSEPLYRAHDILMLRTRELIVACTCAAGCPSCVGPAGDLAPRAK
                     EAALAILERLCNSNGEAR"
     gene            1062..>1275
                     /locus_tag="VII37_03175"
     CDS             1062..>1275
                     /locus_tag="VII37_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068755.1"
                     /translation="MATLDQKFSRLAALKPARPSAVRATTLREPCEEDTMARLLGAGV
                     SINRFGEHLAVRNWFSTPEFVAPHSTC"
BASE COUNT          285 a          342 c          359 g          289 t
ORIGIN      
        1 atcgcaaatg tgcggcgttt gaattgccga ttggacccga ggaccgcttt ggtgacgtgg
       61 atgttcagga catttgcaag cgcttggccg aagcgggctt cttgcaccgc gcgggcgaaa
      121 actatcactg gactcaagaa gcttatcccg ccgataccgt gagtttgcgg tcggtaacca
      181 gcgacaattt tgtgattatc gatatcaccg gcgctccatc ggtgatcgga gaagtagatt
      241 tctctagcgc gctcacaacc gtgcatgaaa aagccattta tatccacgga gggcagcaat
      301 atcacgtgga gcatctggat ttcaaagagc gcaaagccta cgtcaaacgc gtcgatgtgg
      361 aatattacac cgacgcgatt cgctacacgc aggttcgcat cctggaatgc gccgcggaaa
      421 cgcgagtgaa cgactcaatt cctgaaattc cagcactgcg ctccttcgga gatgtgctgg
      481 tgcgctcaca agtgattggg ttcaagaaga tcaagttttt caccaacgaa aatataggcg
      541 ccgggaatct ggaattacct gaaaatgaaa tgcacaccac gtcctactgg atcaccctcg
      601 aacggccgct cctggagtcg ctgcccttta cgataagcga gcggcagagc gggatgtttg
      661 gattgctgca cgccctcgct tccattgcga cgctgctctt gatgtgcgac cgccgcgatc
      721 tgggcactgc cattggggag aaaccgccgt atcccaatga ccacgatgcg gggatagagg
      781 cggtggaact gcgcgatcga atttctcaaa atgccaaggc attctatgag ccgaatttgt
      841 atttgttcga cgcttacccg ggcggaattg gattttccga gccgctatac cgtgcgcatg
      901 acattctgat gctgcggacg cgagagttga tcgtcgcatg tacatgcgca gcaggctgtc
      961 cttcatgtgt ggggcctgcg ggggacttgg ctccgcgagc caaggaagct gcgctcgcga
     1021 ttttggagcg cctgtgcaat tcgaacggcg aggcccgata gatggccaca cttgatcaga
     1081 aattctcgcg cctggcggca ttaaaacctg cgcgcccttc ggcagtacgt gctacgacgc
     1141 tccgagaacc gtgcgaagaa gacactatgg cgcgactcct tggcgccgga gtgtccatca
     1201 accgttttgg cgaacatctt gccgtgcgaa actggttttc taccccggaa tttgttgctc
     1261 ctcactcgac ttgtt
//
LOCUS       DATCBB010000280         1417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_398329, whole genome shotgun sequence.
ACCESSION   DATCBB010000280 DATCBB010000000
VERSION     DATCBB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398329"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..195
                     /locus_tag="VII37_03180"
     tRNA            103..195
                     /locus_tag="VII37_03180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Ser,seq:gct)
     gene            991..>1417
                     /locus_tag="VII37_03185"
     CDS             991..>1417
                     /locus_tag="VII37_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY5068756.1"
                     /translation="MVKSLLLGTAAGLVAMTGAQAADLPVKAKPVQYVKICSLYGAGF
                     YYIPGTDMCLKVGGWVRSYLSWGSNGSNTNGGLTDNLQTRGTGNVDWRERGYITADAR
                     NQTEYGTVRSYIALGVSGDYGRGTPANSGMTFSANRAFIQ"
BASE COUNT          285 a          380 c          441 g          311 t
ORIGIN      
        1 agcttgccgc attagttcgt gtcgcgccaa atttaatgcg gtggcaaatc caaaaccgcg
       61 gtttgaccga ggattgcagt ttctatacgc ctttccgtta ccggagctgt ggccgagtgg
      121 ctgaaggcgg cggtttgcta aaccgttata cgctccaaag gcgtatcgag ggttcgaatc
      181 cctccggctc cgccagcctg cttcgcgctg acacgcttcg caggacttcc gccggccctg
      241 ggggcgcggc gacgcgcttt gcgcgtcgct aaaaattctt caggcaaatt cttttgtgga
      301 tgaatgcatg agccgctcgc gctttcgcga tttgcgggat tggcgcgcgc gtaagtgatt
      361 cgattgcggt cggttggatg actgtccaaa cggttcttgc ggaccgcgat ccgagtcaag
      421 ccctgcatga tgggaaaccg ccgttcgagc caaatgcttt gaaaatgggc ttcccgccga
      481 tttgaggggc aaaaaggtgc cgaaactcga tcatttcggc aaagcgcgcc atcctaaagc
      541 cgcaggtccg attgtcgaat ggatcgtcgg cgatcgtggc tgcatctcat aacctattga
      601 atatgaatga aaatatcagc gatggcgggg tcaggccggc cgctaccgta cggatctggc
      661 caccgccgcg caatttgcgt gttttttgcg caacactcat ttgaaaagcg tcctcgggac
      721 ctgtgggaat ggcgcttctt cattgcgccg cagacttcat tcgataatgt ctgtggtgcg
      781 acggaggggg gcatccccgt gtcgcgcttt ccgggttgac ggatcgcacc gagtggcaag
      841 cccaggtttg gctttcaaag ttgggtcgat cgcagggtgc gaaaagtttg tccgggtggc
      901 ggggagccag gggaagttcc atcggtgcct gtcgcactgc ggatacagaa ccctctcacg
      961 ataaacaacg tttggaggtt caagatgaag atggtgaaaa gcctactcct gggcaccgcg
     1021 gcgggtctcg ttgcgatgac tggggcgcag gccgccgatc ttcccgtaaa ggccaagccg
     1081 gtccagtacg tgaagatttg cagtctgtat ggtgcggggt tctattacat cccgggcacc
     1141 gatatgtgct tgaaagtcgg cggttgggtt cgttcgtacc ttagttgggg ctcgaacggc
     1201 agcaacacga acggcggact taccgacaat ctgcaaacgc gcgggacggg caacgtggat
     1261 tggcgtgagc gcggctacat caccgcggac gcgcgcaacc agaccgaata cggcacagtt
     1321 cgtagctaca tcgcccttgg tgtaagcggt gactacgggc gggggacacc ggcaaactcc
     1381 ggaatgacct tcagcgccaa ccgcgcgttc atccagt
//
LOCUS       DATCBB010000281         1303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_8946, whole genome shotgun sequence.
ACCESSION   DATCBB010000281 DATCBB010000000
VERSION     DATCBB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8946"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VII37_03190"
     CDS             <1..286
                     /locus_tag="VII37_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068757.1"
                     /translation="FFPGVTPVRLPVQVTALRGGANRLREASLIEFAGVEHAILVSGL
                     PLEFDDKVRLEGSRKADTADATVVAVQYHGGQKAVAVKFLRGHNRWIMNS"
     gene            283..>1303
                     /locus_tag="VII37_03195"
     CDS             283..>1303
                     /locus_tag="VII37_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068758.1"
                     /translation="MSNLTSPSMPGTAGTTQSQWAETVSVFPIYLALAKQLGISVPFL
                     QRKLPETPDPELSGQVYSWLDAMDQRVLVYQLRHLLQMTTLNASESSLKSLIERHLRK
                     ATKTTVDRDKIDFLLVQYFALSAPAKIYHKRIEPLDVAEVMKPILGEVAPASLSWCQP
                     LEAMIQSLHSFRSLREVLKTNFIEQGRKVKEVAAGMFYDPSALLAFIRFNFILRRTLI
                     ELMHADLIATRAGITRLEALDVHVVDCRNTGLSAAEPLTKVKKLADEWKQPFQKEYTE
                     RSVNQAFEKLLALRADVENALEKSAAKTSDRLGVDPGKGSSPESRKIKDSEPAPLSAL
                     HPKSNG"
BASE COUNT          308 a          360 c          359 g          276 t
ORIGIN      
        1 atttttcccc ggagttacgc cggttcgctt gccggtacaa gtgaccgcgc tgcggggcgg
       61 ggcaaaccgg ctgcgggaag ccagcctgat agagtttgca ggggtggagc atgccatatt
      121 ggtctccggc ttgccgcttg aatttgacga taaggtacga ttggaaggaa gccgcaaagc
      181 ggacactgcg gacgccaccg tggtcgccgt ccaatatcac ggcggacaaa aagccgtggc
      241 cgttaaattt ctacggggtc acaaccgctg gataatgaac tcatgagcaa tctcacatca
      301 ccatcgatgc ccgggactgc gggaacaacc cagagtcagt gggcggaaac agtttccgtt
      361 tttccgattt acttagccct ggctaaacag ttggggattt ccgtcccatt tctgcagcga
      421 aagttgcccg aaacgccgga tccagaactc tccggccaag tttacagctg gctcgacgcg
      481 atggatcagc gcgtcctcgt ctaccaattg cgccatcttt tgcagatgac cacgttgaac
      541 gcaagcgaga gcagcttgaa atcgctcatc gaacggcact tgcgcaaagc aacgaagacg
      601 accgtcgacc gcgacaagat cgactttctt ctggtgcaat acttcgccct ctctgcgccc
      661 gccaagattt atcacaagcg catcgagcct ttggacgtcg ctgaggtgat gaaaccgatt
      721 cttggcgaag tagcgcctgc atctctctca tggtgtcaac ctttggaagc catgattcag
      781 tccctacaca gttttcgaag cctgcgcgaa gtgctgaaaa ccaatttcat cgagcagggc
      841 cgcaaggtta aggaagtggc cgccggaatg ttctacgatc cgtccgcgtt gctggctttt
      901 attcgtttta attttatctt gcgccgcacg ctgattgagt tgatgcacgc cgacctgatc
      961 gccacgcgtg caggtataac ccggctcgag gcattggatg ttcacgtggt cgattgccgc
     1021 aatactggtc tgtccgcggc cgagccactg accaaagtca agaaacttgc cgacgaatgg
     1081 aaacaacctt tccaaaaaga atataccgag cgatcggtga accaggcttt tgagaaattg
     1141 ctcgcgttgc gcgcggatgt ggaaaatgcg ttggaaaaga gcgccgcgaa aacgtctgac
     1201 cggctgggcg tcgatccggg aaaaggaagt agccccgagt cccggaaaat aaaggacagc
     1261 gagcctgcgc cgctatccgc cctgcaccct aaaagcaacg gcc
//
LOCUS       DATCBB010000282         1162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_73348, whole genome shotgun sequence.
ACCESSION   DATCBB010000282 DATCBB010000000
VERSION     DATCBB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73348"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VII37_03200"
     CDS             complement(<1..205)
                     /locus_tag="VII37_03200"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY5068759.1"
                     /translation="MKRALITGITGQDGSYLAELLLSKGYEVHGIIRRSSTFNTQRID
                     HIYQDPNDPNARLFLHHGDLSSTE"
     gene            complement(330..>1162)
                     /locus_tag="VII37_03205"
     CDS             complement(330..>1162)
                     /locus_tag="VII37_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY5068760.1"
                     /translation="KINCAAIPSGLLESELFGHERGAFTGALNQKIGRFELADHGTLF
                     LDEVGDLPMELQPKLLRVLQEHEFERLGSNRTIKVDVRVVAATNQDLAKQVADRTFRS
                     DLYYRLNVFPIQIPALRERREDVPLLIRYFVQKFSRRLNKVVEYVPAEAMTALANYSW
                     PGNVRELENFVERAVLLSPGKELRVPVSELLSEPGSPSTERSSRRAVALRTGDNNDEA
                     TSSSAISTLEEAEREHILRALQQTQWRIGGPKGAAALLDMKRTTLQARMRKLNIRRPV
                     "
BASE COUNT          268 a          329 c          304 g          261 t
ORIGIN      
        1 actccgtgct ggacaaatcg ccgtggtgca aaaacaagcg cgcgttcgga tcgttggggt
       61 cttgatagat gtgatctatg cgctgagtat taaacgtgct ggaacgccgg ataatcccat
      121 gcacttcata gccctttgaa agcaacagct ccgccaggta ggaaccatcc tgaccagtaa
      181 tgcccgtaat gagcgcccgc ttcataattt caagatctcc cgtaggtcag cggcgtagtg
      241 ccgtcagctt cccgtcgcag cgcaattgta gcttagctgc tgtgattgcc gtgtgcccga
      301 aaattgctgg ttaacactcg aaaaaatatc tataccggac ggcgaatgtt taattttctc
      361 atgcgcgcct gcagcgtggt acgtttcatg tccagaagag cggcagctcc tttcggaccg
      421 ccgatgcgcc attgagtttg ttgcaaagct cggagaatat gctcgcgttc ggcttcttca
      481 agtgtggata tggcagagga agatgttgct tcatcgttgt tgtcccccgt tcggagagcc
      541 actgcgcgac gagacgaacg ctccgttgaa ggcgaccccg gctcggacaa caactcggaa
      601 accggaacgc gcaattcttt tccgggcgac agcagcacgg cgcgctccac gaaattctca
      661 agctcccgca cattccccgg ccaggaataa tttgccagcg cggtcatggc ctccgcgggc
      721 acatactcca caaccttatt cagccggcgg ctgaattttt gaacgaaata gcgtatcaac
      781 agcggtacat cctcgcggcg ctctcgcaaa gccggtatct gaatcgggaa aacattcaag
      841 cggtaataaa ggtcgctgcg aaaagtacga tcggcaactt gtttggccag gtcctgattc
      901 gtggccgcca ccacccgtac atccactttt atggtgcgat tgcttcccaa ccgttcgaac
      961 tcgtgctcct gcaaaacgcg gagcagctta ggctgcagct ccatgggcag atcgccgact
     1021 tcgtcgagga acaacgtgcc gtgatcggct aattcaaaac gcccaatttt ttgattcagc
     1081 gccccggtaa aagcgccgcg ctcgtgtcca aaaagctcgg actccaacag cccgctggga
     1141 atggcggcgc aattaatttt ga
//
LOCUS       DATCBB010000283         1169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_301089, whole genome shotgun sequence.
ACCESSION   DATCBB010000283 DATCBB010000000
VERSION     DATCBB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301089"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..609
                     /gene="def"
                     /locus_tag="VII37_03210"
     CDS             115..609
                     /gene="def"
                     /locus_tag="VII37_03210"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5068761.1"
                     /translation="MKFGDPVLDTPAAEVTSFDDEIKKLVEDMFESMYAARGVGLAAP
                     QIGISKRIAVVDVTFKEEPDAKLVLINPVIVHKEGRQRSSEGCLSIPEFREDVTRAKI
                     VTIRAQDLKGKFFEHTGEELLARAFLHETDHLNGKLYISHISGIKRDLIKRKIKKLVK
                     AGEW"
     gene            747..>1169
                     /locus_tag="VII37_03215"
     CDS             747..>1169
                     /locus_tag="VII37_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5068762.1"
                     /translation="MPNALTVVFCGTPQFAVPTLERLVAAGHSVPLVVTQPDRPRGRG
                     MEIAVSPVKAAATRLGIEVLQPAAIKNNAEFRERLAAIAPDAIIVVGYGRIIPQWMID
                     LPRLGNLNLHGSLLPKYRGAAPIQWAIASGESVTGVTTM"
BASE COUNT          277 a          294 c          344 g          254 t
ORIGIN      
        1 cggcgactcg atgagggaaa cgaaacgaaa tttccaggga tatctggaac aattgaaaaa
       61 ctactaaggc aaagtaatga ccgaagttaa tttacgaaga tgatttatcc cattgtgaaa
      121 ttcggcgatc ctgtgctcga cactcccgct gcggaagtga ccagcttcga tgatgagatt
      181 aagaagcttg tggaggatat gttcgagtcg atgtacgcgg ctcggggcgt cggcctggct
      241 gctccgcaga tcggcatttc gaagcggatt gcggtggttg atgtgacctt caaggaagag
      301 ccggacgcaa agctggtgct gatcaatccg gtgatcgttc acaaggaagg gcggcaacgc
      361 agcagcgagg gctgcctcag cattcccgaa tttcgcgagg acgtaacccg cgccaaaatc
      421 gtgacaatac gggcacagga tttgaagggc aagttttttg agcatacggg agaagagctt
      481 ctggcccgcg ccttcctgca tgagaccgac cacctcaacg gcaaactgta tatctcccat
      541 atcagcggca taaagcgcga tctgatcaag cgcaagatca agaaactggt gaaagcggga
      601 gagtggtaga gatttcgcaa tttggcgatt ttgtaatttg gaggcgctta atttcggctg
      661 gcacttgtta ccgccgggta cctcagcggc taaagcctcg ttcgaacaat tgaaaaccgc
      721 agcgctgaag cgctgcgcca cccaaaatgc ccaacgctct tactgtcgtt ttttgcggca
      781 cgccgcaatt cgccgtcccc acgctggaaa gactcgtcgc ggctggccat tctgtgccgc
      841 tggtggtcac gcaaccagac cgtcctcggg ggcgtggaat ggaaatcgca gtttctcctg
      901 tgaaagcggc ggcaacgcgg ttgggcatcg aggtattgca gccggccgcc atcaagaaca
      961 atgccgaatt tcgcgagcgg cttgcggcga ttgctccgga cgcgatcatt gtggtcggct
     1021 atggccgcat tattccgcag tggatgatcg atctaccacg gcttggaaat ttaaatttgc
     1081 atgggtcgct gctgccaaag tatcgcggcg cagctcctat tcaatgggcg atcgcgagcg
     1141 gtgaatcggt gactggcgtg actaccatg
//
LOCUS       DATCBB010000284         1490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_268627, whole genome shotgun sequence.
ACCESSION   DATCBB010000284 DATCBB010000000
VERSION     DATCBB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268627"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VII37_03220"
     CDS             complement(<1..304)
                     /locus_tag="VII37_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068763.1"
                     /translation="MKTRLPVRKAILQRKTYEAPAEGRLNKMRLDFNENTTGCGPAVL
                     RALRKLTPQQLAMYPEYAKPTRRIAAYFGVSEHELLLTNGGDDALRVFFDAFVEPRN"
     gene            complement(301..>1490)
                     /gene="hisD"
                     /locus_tag="VII37_03225"
     CDS             complement(301..>1490)
                     /gene="hisD"
                     /locus_tag="VII37_03225"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5068764.1"
                     /translation="DEKAHRVAGRIVADVRKRGDVAIREWTNKFDGVDLRAGMWVSSR
                     EIEKVANDVAKETRIAIEHAARNIRAVAERQLPREWTLEVEPGIEISQVVRSIEAIGC
                     YVPGGRFALLSTLLMTVIPAQVAGVKRIVVVSPKPNAELLAAAAMLGVTHIARIGGAQ
                     AIAALAYGTKSIAAVDKIFGPGNRFVTAAKQLVSGDCAIDLPAGPTEAIVLADDGDPA
                     WIAADLLAQAEHAPDAGSFLVTTSRDLAARTQVEIQKQLRALPKTNPAQQSSARSGAI
                     LLAPSMERACEFVNRFAPEHLSLPQNTDELLQKVQSSGTVFVGPWSAQAFGDYASGSN
                     HVLPTAGWARARGGLSTADFVKSISVQHISETGFARLAQDVQILARAEGLLAHANAVE
                     VRR"
BASE COUNT          320 a          486 c          373 g          311 t
ORIGIN      
        1 aattgcgagg ttcgacgaac gcatcgaaaa atactcgcaa cgcatcgtct cctccgttgg
       61 taagcaacaa ttcatgctcc gaaactccga agtacgcagc gatgcgtcgc gttggcttgg
      121 catattccgg atacatcgcc aattgttgcg gagtaagttt cctgagagcg cgcagtaccg
      181 ccgggccaca ccccgtagtg ttctcattga aatccagccg cattttattc agccggcctt
      241 ctgcaggtgc ctcatacgtt ttgcgctgca agatggcttt gcgtaccggc agccgcgtct
      301 tcatcggcgc acctcgactg cattcgcgtg cgccagcaac ccctcggctc gtgccagaat
      361 ttgaacgtcc tgtgccagtc gcgcaaatcc cgtctcgctg atgtgctgca cgctaatgct
      421 tttcacgaag tccgcagtgc tgagtccgcc gcgtgcccgt gcccatcccg ccgtaggcaa
      481 aacgtgatta cttccactcg cataatcgcc aaatgcctgc gcgctccacg gaccaacaaa
      541 aaccgttcca gaagattgaa ccttttggag gagctcatcg gtgttctgcg gcaaactcaa
      601 gtgctcaggc gcgaagcggt tcacaaactc acaggcccgc tccatcgaag gagccaacaa
      661 aattgcgccg ctccgcgccg aggactgttg tgcgggattc gtcttcggca atgcgcgcag
      721 ctgcttctga atctcaactt gagtgcgagc agccagatca cgagaagtgg taaccaggaa
      781 gctcccagca tcgggagcgt gctcggcttg ggccagcaaa tccgccgcga tccaggccgg
      841 atcaccatca tctgccaaca caatcgcctc tgtaggcccc gccggaagat cgatcgcgca
      901 atcgccactc accagttgct tcgctgcggt gacgaaccga ttccccggcc caaaaatttt
      961 atccaccgcc gcgatgctct tcgtaccgta tgcgagcgcg gcaatcgcct gtgcgccgcc
     1021 aatccgggcg atatgcgtaa cacccaacat cgccgctgcg gccaacagtt cagcatttgg
     1081 ttttggagag acgaccacaa tgcgtttcac gccagccacc tgagccggaa tcaccgtcat
     1141 cagcaacgtc gatagcagcg caaaacgtcc gccaggcaca taacagccaa tggcttcgat
     1201 agaccgaaca acttggctga tttctatccc aggctcaacc tccagcgtcc attcgcgtgg
     1261 caattggcgc tcggcgacag cacgaatgtt ccgggccgca tgctctatcg cgatgcgcgt
     1321 ctctttggcg acatcgttcg caactttttc gatctctcga gaggaaaccc acatcccggc
     1381 tcgcaagtcc acaccatcaa atttgttagt ccattcgcga atcgccacat cgccacgctt
     1441 ccgcacatcg gcgacgatgc gtcccgcgac gcggtgtgct ttttcatccc
//
LOCUS       DATCBB010000285         1692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_171320, whole genome shotgun sequence.
ACCESSION   DATCBB010000285 DATCBB010000000
VERSION     DATCBB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171320"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..764
                     /locus_tag="VII37_03230"
     CDS             138..764
                     /locus_tag="VII37_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068765.1"
                     /translation="MKAFRKLLARTPFYGVYKLLGHYPDYWYWKLRGEPIRSPHLLKQ
                     RTVREYAQRFGLRVLVETGTYYGEMVDAMKNRFAEIHSVEFDSALAHRAAKKFSRWPH
                     LHIHEGDSQMVVPELLKSLKEPALFWLDAGYYGWAGLQGDKQRLTSELESILRDSRYR
                     HVILMDDARGLNGQNGAPTVPQVKQRIEAEFPGRSVDVKHDILRIVPG"
     gene            874..>1692
                     /locus_tag="VII37_03235"
     CDS             874..>1692
                     /locus_tag="VII37_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068766.1"
                     /translation="MSFLRDLKIAIRSLTRARALWFTVAITLALGIGANAAIFSVIRG
                     VLLRPLANRDEDRLLYVRQSAPGIGAENTTFSVPEIKDMDSGLKSIKEFGTFSAVDFT
                     IVGLGDPREIRAGVVDGNYFDVMGLRPVLGRLLNPGDDGQKAAGAAVLTYRFWVNSLH
                     SDRGVIGKVVRLGGFEGSRSATIVGVLEPSVPYPVETEMIANIVTSPHHLSATMVTGR
                     EHRMTEVFARLAPGADLEAARAELRTVHAAMVSAHPEVYKPADHYKIEVTRMHDQ"
BASE COUNT          349 a          464 c          487 g          392 t
ORIGIN      
        1 gcggtactga ggtaaaaaca tccacctgca cgaacggtaa acggcgtttc tggtttgaca
       61 tggttttccc cgcaccctta tgctcgaaag ttcagtgaag gcgccaaatt tcagggcgcg
      121 taggcttaat actaccaatg aaagctttcc gcaaattgct ggcgcgcaca cccttttacg
      181 gtgtttacaa attgctgggc cattaccctg actactggta ctggaagctg cgcggtgagc
      241 ccattcgctc tccgcatttg ctcaagcaac gcaccgtccg cgagtatgcg cagcgttttg
      301 gcttgcgcgt gctggttgaa accggcactt attacggcga gatggtggac gccatgaaga
      361 atcgttttgc ggaaatccat tccgtagaat tcgattctgc gctggcgcac cgcgccgcaa
      421 aaaagttttc tcggtggcca cacttgcata ttcatgaagg tgacagccag atggtggtgc
      481 cggaacttct gaagtcgctg aaggagcctg cgctcttctg gcttgatgcc ggctactatg
      541 gctgggccgg ccttcagggc gacaagcagc ggctcaccag tgaactggaa tcgatcctgc
      601 gagattcgcg ataccggcac gtgattctga tggatgacgc gcgtggcctc aatggccaga
      661 acggcgcgcc cacggtaccg caagtcaagc agcgaatcga agcggagttc ccaggcagga
      721 gcgtggatgt gaagcatgac attctgagga ttgttccagg gtaagtaaat gttttttgcc
      781 gcttctgtaa tctctggttg tacgtctaat ttaaatcagc gaaactgttt acgccgctca
      841 gagcgacact aaccacacca atggaggaca gccatgtctt ttcttcgcga tttgaaaatt
      901 gcgatacgtt ctttaacccg agcgcgggca ctgtggttca ccgtagctat caccttggct
      961 ctcggtatcg gcgccaacgc ggccatcttc agcgttattc gcggcgtgct cctgcgtccg
     1021 ctggcgaatc gcgatgaaga tcgccttctg tacgtgcgtc agagcgctcc gggaatcggc
     1081 gcggagaata ccaccttctc agttcctgaa attaaggaca tggactccgg cttgaagagc
     1141 atcaaggagt tcggcacttt ttctgcggtc gatttcacca tcgttgggct gggcgaccca
     1201 cgtgagatcc gcgctggtgt ggttgacggc aattattttg acgtgatggg cctgcgtccg
     1261 gtgttaggac gcctgctcaa tcccggcgac gatggccaaa aagccgcggg cgcagctgta
     1321 ctgacctacc ggttctgggt gaactcgctg cattccgatc gcggcgtaat cgggaaagta
     1381 gtccgtcttg gagggttcga gggcagccgt tccgccacga tcgttggagt cttggagcca
     1441 tccgttcctt atccggtgga aaccgagatg attgccaaca tcgttaccag tccgcatcat
     1501 ctctccgcga ccatggtgac cggccgcgag catcgcatga ccgaggtctt tgcgcgtctt
     1561 gctcccggcg cagacctgga agctgcccgg gcggagctgc gcaccgtgca tgcggccatg
     1621 gtgtccgcgc atcctgaggt ttacaagccg gcggaccact acaaaatcga agtaacgcgc
     1681 atgcatgatc ag
//
LOCUS       DATCBB010000286         1168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_171362, whole genome shotgun sequence.
ACCESSION   DATCBB010000286 DATCBB010000000
VERSION     DATCBB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171362"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..>1168
                     /locus_tag="VII37_03240"
     CDS             5..>1168
                     /locus_tag="VII37_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY5068767.1"
                     /translation="MRAQGYNMTLGGGTNITREPRNGRTFEYLGEDPVLAGTLVGNRI
                     KCEQAQHVIGDIKHYAVNDQETGRNEINAVIGKRALQESDLLAFHIGIEIGDPGAVMC
                     SYNALNGDYACENKYLLTDVLKKAWKFPGFVLSDWGGTHSVVKASAAGLDNEEPEDIY
                     YGEPLKKAVQDGKVPVAELDEHVRRILRSEFATGIVDFPKKKSVVDVERGLETARQVA
                     EQSIVLLKNDKGILPLDRAKVRSIAIIGTHADEGMISGGGSAQVDPPGTTAPKWKAHV
                     WFPTSPLKAVAAKAPGVSVKFASGEDPAATAALAKQSDVAIVFVHQWTSEDFDLPDLS
                     LPDNQDAMIDQVASANPHTIVVIESGSSVTMPWVDKVSGVLEAWYAGSKGADAV"
BASE COUNT          276 a          249 c          396 g          247 t
ORIGIN      
        1 cgaattgcga gcgcaggggt acaacatgac tctaggcggc ggcacgaaca ttacgcggga
       61 gccgcgcaac ggacgtacgt tcgaatacct gggagaagat ccggttttgg caggcaccct
      121 ggtgggcaac cgcataaagt gcgagcaggc gcaacacgtt atcggggaca tcaaacatta
      181 tgcggtcaac gatcaggaaa ctgggcggaa tgaaataaat gcggtgatcg gcaagcgcgc
      241 gctgcaagag tcggacttgc tagcatttca tattggcatc gagattgggg atccgggcgc
      301 ggtgatgtgt tcctacaatg cgttgaacgg ggattatgcg tgcgagaaca aatatctgct
      361 tactgatgtg ctgaagaaag cgtggaagtt cccaggattc gttctttcgg attggggcgg
      421 gacgcatagc gtggtgaaag catctgcggc tggtttagat aacgaggagc cggaagatat
      481 ttattatggc gagccgctga agaaagcggt gcaggacggc aaggttcccg ttgcggaact
      541 ggatgagcat gtgcggcgta ttctgaggtc tgaatttgcc actggcatcg tggactttcc
      601 gaagaaaaaa agtgtggtgg atgttgaacg cggattggaa actgcgcggc aggttgcgga
      661 acaaagtatc gtgctgctga aaaacgacaa ggggatattg ccattggatc gcgcaaaagt
      721 gcgctctatc gcgattatcg gaactcatgc ggacgaaggc atgatttcgg gcgggggatc
      781 ggcgcaagtg gatccgcctg ggactacggc acctaaatgg aaggcgcatg tgtggtttcc
      841 tacttcgccg ttgaaggcgg tggccgcgaa agctccggga gtgagtgtga agtttgcatc
      901 aggggaggat cctgcggcaa ctgcggcgct ggctaagcag tcagatgtgg ccattgtgtt
      961 tgtgcatcag tggaccagcg aggattttga cttgccggat ttgtctttgc cggataacca
     1021 ggacgcgatg attgaccagg tggcttcggc aaatccgcac accatcgtgg tgatcgaatc
     1081 tggaagctcc gtaaccatgc catgggtcga taaagtcagc ggagtgctgg aagcctggta
     1141 tgccgggagc aagggcgcgg acgcggtg
//
LOCUS       DATCBB010000287         1385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_333245, whole genome shotgun sequence.
ACCESSION   DATCBB010000287 DATCBB010000000
VERSION     DATCBB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333245"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /gene="sthA"
                     /locus_tag="VII37_03245"
     CDS             <1..1309
                     /gene="sthA"
                     /locus_tag="VII37_03245"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEY5068768.1"
                     /translation="KRVAIVESQSSVGGVCINTGTIPSKTMREAVLHLSGFYDKNFYG
                     VSHTNGNEPVAMADLNFRVQHVVENEVGVIQDQLKRNGVVLIHGTGSFRDAHHIRVQG
                     INSVGEFEADFVVIATGTKPAVNPKVPINGRNIISSDQILSMPQIPRTLIVVGGGVIG
                     VEYACMFATLGVRVIIVEKRPRLLEFADTEMVEALMYHLRDNRATLRLNEEVESVEEL
                     PDGKVAANLVSKKRVIADALLYAVGRHGNVEGLNLEAAGISSDDRGRITVDSDFRTTQ
                     KHVFAVGDVIGFPSLASVSMEQGRIASARAFGLPVSSDPEGYPYGIYTIPQISFIGKT
                     EEQLTESDVPYEVGVAYYREIARGQISGHTEGRLKLLFHRETLELLGVHIFGEDAAEL
                     LHIGQAVFLLKGKVTYFVNTVFNYPTLAECYKTAAFNGLNRLQ"
BASE COUNT          325 a          320 c          431 g          309 t
ORIGIN      
        1 caagcgagtg gctattgtgg aaagccaatc gtcggtgggc ggggtgtgca tcaatacggg
       61 aacaattccc agtaagacga tgcgcgaagc ggtcctacac ctgtcaggtt tttacgacaa
      121 gaatttttac ggcgtcagcc acaccaacgg caatgagccg gtggccatgg cggacctgaa
      181 tttccgagtg caacacgtgg tggaaaatga agtgggcgtc atccaggatc aactgaagcg
      241 caatggcgtg gtgctgattc acggcaccgg aagctttcgc gacgcgcacc atatccgcgt
      301 acagggtatt aatagcgtgg gagagttcga agctgatttt gtggtaattg ccacgggaac
      361 gaagcctgca gtgaatccca aagtgcccat caacgggcgg aacattatca gcagcgacca
      421 aattttgagc atgccgcaga tcccgcgcac tctgatcgtc gtgggcggcg gtgtgatcgg
      481 cgtggagtac gcatgcatgt tcgcgacgct gggcgtgcgc gtgatcattg tggagaagcg
      541 gccgcggcta ctggagtttg ctgacaccga aatggtggaa gcgctgatgt atcacttgcg
      601 cgataaccgg gcgactttgc ggctgaatga agaagtagaa agtgtcgagg agttgcccga
      661 cggcaaagtg gccgccaacc tggtcagcaa gaaacgagtg attgctgatg cgctgctcta
      721 tgcggtgggg cgtcacggaa atgtcgaagg tttaaattta gaggcagcgg gtatttcttc
      781 ggacgatcgg ggacgaatca ccgtggactc ggatttccgt accacgcaga aacacgtctt
      841 tgccgttggt gacgtaattg gatttccaag tttggcttcg gtgtctatgg aacaggggcg
      901 aatcgccagc gctcgggcgt tcgggcttcc agtgagcagc gatccggagg ggtatccata
      961 cggcatttac accatcccgc agatttcgtt tattggaaaa acggaggagc aactgaccga
     1021 gtcggatgtt ccgtatgagg tgggcgtggc ctattaccgg gagatcgcac gcgggcagat
     1081 cagcgggcat accgaaggca gattgaaatt attgtttcac cgcgagacgc tggaattgct
     1141 gggcgtgcat atttttggtg aagatgcggc ggaattgttg catatcggcc aggcggtgtt
     1201 tttgttgaag ggcaaagtga cctatttcgt gaataccgtt tttaattacc cgactctggc
     1261 ggaatgctat aagaccgcgg cgtttaatgg attgaaccgg ctgcagtaaa actgccgcct
     1321 ggatttagtc accgcacatc gcaaacttca caaaaagtag acttcccgtg agccccgttc
     1381 caaga
//
LOCUS       DATCBB010000288         1052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_398561, whole genome shotgun sequence.
ACCESSION   DATCBB010000288 DATCBB010000000
VERSION     DATCBB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398561"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /gene="xrt"
                     /locus_tag="VII37_03250"
     CDS             complement(<1..933)
                     /gene="xrt"
                     /locus_tag="VII37_03250"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HEY5068769.1"
                     /translation="MAFVYFTVLLKLGGDWWNDENYSHGLLIPFVIGYILWQERERFT
                     EARTQPAVWWGAIGVGFSLLLLWAGTAGAELFVQRVSLVLMVASVVIYFWGFRLLRLI
                     AVPLSLLILAIPIPQIIFNRIAFPLQLFASRCAVAAMSFFSIPVLRQGNIIELMPLGA
                     SEPKRLAVVEACSGIRSLMTLVTLAVVYAYFTKPKSDRGNGGDDDGATPKEMRTRRPR
                     SQHKGVAFFKSFTFWRSLILVVAAVPIAILTNALRVSGTGVLAHYYGTRVADGFFHSF
                     SGWVIYIAAAVLLFATGWILDRLAGFGKKDHDMIY"
BASE COUNT          265 a          304 c          295 g          188 t
ORIGIN      
        1 gtaaatcatg tcatgatctt tctttccgaa acccgcgaga cgatccagta tccatccggt
       61 ggcgaacagc agcaccgccg ccgcgatgta gatcacccag ccagagaaag aatggaagaa
      121 accatcggcc acgcgcgtgc cgtagtagtg cgccagcacg ccggtgccac tgacgcgtaa
      181 agcgttcgtt aggattgcga tcgggactgc cgccacaacc agaatcagcg agcgccaaaa
      241 cgtaaaactc ttgaagaaag cgacaccttt atgctgggaa cgcggacgtc tcgtccgcat
      301 ttcctttggt gtggcgccat catcatcacc gccgtttcct cgatcgcttt tcggtttcgt
      361 gaagtacgca taaaccacgg cgagcgtcac cagcgtcatc agcgaacgta tgccgctgca
      421 cgcttcgact acggcgagtc ttttaggttc gctggcgccg aggggcatca gctcgattat
      481 gtttccctga cgcagcaccg gaatgctgaa gaaagacatt gcggccacgg cacagcgcga
      541 cgcgaagagc tgcaaaggaa aggcaatccg gttaaagatg atctggggaa tcgggatcgc
      601 cagaattaat agcgatagag gcaccgcgat cagacgcagc aggcggaagc cccaaaagta
      661 aatcaccacg ctggcgacca ttaacaccag cgaaacacgc tgtacgaaaa gctcggcacc
      721 ggctgttcct gcccacagca gcagcaaaga aaacccgacg ccaatcgcac cccaccagac
      781 agcaggctgt gttctggctt cggtgaaacg ctctcgctcc tgccaaagga tatagccgat
      841 gacgaaggga atcaggaggc cgtgcgaata gttctcgtca ttccaccagt cgccacccaa
      901 cttcaggagc acggtgaaat agacaaacgc caacgcgatg gcgatggtta acggttggta
      961 aagctttcgc gggacggcga cactcatgcg gtttggtgct ttcgcggccg aggggcaatc
     1021 tctgccggcg aagcggttat cgatgcgggc cg
//
LOCUS       DATCBB010000289         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_301282, whole genome shotgun sequence.
ACCESSION   DATCBB010000289 DATCBB010000000
VERSION     DATCBB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301282"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VII37_03255"
     CDS             complement(<1..1524)
                     /locus_tag="VII37_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068770.1"
                     /translation="MGTITLAPPEKQSSPSPASPSQPQSTWIYGAWIDLTVGCGAWSA
                     PLLLAGFYFANSHARGWSVAFYFLALLSNYPHFMATVYRAYHTRDEFEKYRLYTVHVA
                     LLLAVAGVVTHLWYALLPWIFTVYICWSPWHYTGQNFGLLMMFARRASVTPTETERRA
                     LRLSFIASYILLMLSFHTGASGDPLILSLGLAAKFTLPARAALALFFLGASGWALASL
                     ARRSNFRSVIPAVTLTVTQFLWFLLPAVIELVSGREIPQTRYSSGLLAVLHSTQYLWI
                     TSYYQKKEARAAGDSRWSFSHYLVTLIAGGIALFIPGPWIVSRVFHADFAASFLTFTA
                     LVNIHHFILDGAIWKLRDSRIAAVLLDGKQKPTHSVDEKQNSFARAARWLTGRTPAAR
                     AFRIATVALLFVWAAVDQLHFWWSSEASALPVLERAAALNPDDSSVQVRLAHAEQLAG
                     QRDAALAAMQRAASLNTANLALQESYGRSLIEAGRDADAYMQFRQIIARWPRNADALV
                     "
     gene            1674..>2202
                     /locus_tag="VII37_03260"
     CDS             1674..>2202
                     /locus_tag="VII37_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY5068771.1"
                     /translation="MAKRSLTKQLNLIPTKASLTELRQAAKSCKNCDLWKLATQTIFG
                     EGAAHSKVMLVGEQPGDQEDQAGKPFVGPAGRLLDTALVAAGIDRKKVYITNAVKHFK
                     WEPRGKRRIHKKPNAAEIAACRPWLDSEIAAISPKVIVCLGATAAQALLGKDFRVTQH
                     RGEFIESSLAPYVAAT"
BASE COUNT          546 a          661 c          620 g          375 t
ORIGIN      
        1 aaccagagcg tcggcgttac gcggccagcg tgcaatgatt tgtcggaact gcatatacgc
       61 atcggcgtcg cgcccggctt caatcaaact ccggccataa gactcttgca acgccaggtt
      121 tgcggtattg agcgaagccg cacgctgcat ggccgcgaga gcggcatcgc gctgcccggc
      181 aagttgctca gcgtgggcga gacggacttg caccgagctg tcgtcaggat tcagggccgc
      241 ggcgcgctca agtaccggga gagcgctcgc ctcgctggac caccagaaat gcagttggtc
      301 cacggcagcc caaacgaata gcaacgccac cgtcgcgata cggaaggcac gagcggcagg
      361 ggtgcgccct gttagccagc gagcagctcg cgcaaagcta ttttgtttct cgtctacaga
      421 atgtgttggt ttctgcttac cgtccaggag cactgcagca atgcgcgaat cgcgcaattt
      481 ccagatggcc ccgtccagaa taaagtggtg aatatttacg agcgccgtga atgtaagaaa
      541 actcgcggca aaatcagcgt gaaaaacccg gctgacaatc caaggcccgg ggatgaatag
      601 cgcgattccg ccggcaatca gtgtcaccag ataatgcgaa aacgaccaac gggagtcgcc
      661 agccgcacgc gcttcttttt tctggtaata actggtaatc cacaaatact gcgtggaatg
      721 gagcaccgcg agcagcccgc tgctgtaacg cgtctgcgga atttcgcggc cgctgactaa
      781 ctcaattacc gctggcaaaa gaaaccagag aaactgtgtc accgtcagcg taacggccgg
      841 aataacgctg cggaagttgc ttctgcgcgc cagcgaagcg agagcccagc cactggcacc
      901 caaaaaaaag agcgccagcg ccgctcgtgc ggggagagtg aatttagcag cgagccctag
      961 cgacagaata agcgggtcgc cggacgcacc cgtgtgaaaa ctcagcatca gcaaaatgta
     1021 ggaggcgatg aaggaaagcc ggagcgcgcg gcgttctgtt tccgtgggtg tcacgctcgc
     1081 gcgccgcgcg aacatcatca gcaatccgaa attttgtccc gtgtagtgcc acgggctcca
     1141 gcaaatgtag accgtgaaga tccatggaag cagcgcatac caaaggtgcg tgacaactcc
     1201 ggccacagcc agcagcagcg ccacatgcac cgtatacagg cggtatttct cgaactcgtc
     1261 gcgcgtgtgg tacgcgcggt acaccgtggc catgaagtgc ggatagtttg aaagcagcgc
     1321 cagaaaataa aacgccacgc tccagccgcg cgcgtgggag ttcgcaaaat aaaatcctgc
     1381 cagcaagagc ggcgcggacc aagcgccgca gcctaccgtc aagtcaatcc atgcaccata
     1441 aatccaggtg ctctgcggct gagacggcga ggcaggcgac ggtgaactct gcttctccgg
     1501 aggagctaag gtgatggttc ccatgctatc gcagagtatc gcagccgtgg cgggctttgg
     1561 cttcgagggc cgtcaccgca tgcggaatcg gttacgtgta cataccacgc ctctaagcct
     1621 gcccccctca cacgctttga cgcatcctaa tttggaactg agggaagcca agcatggcta
     1681 agcgcagtct aaccaagcag ctgaatctaa ttcccaccaa agcctcgcta accgaattgc
     1741 gtcaagccgc gaaatcctgc aaaaattgcg acctctggaa gctcgccacg caaaccatct
     1801 tcggcgaagg cgccgcgcac agcaaagtaa tgctcgtcgg agagcaaccc ggcgaccagg
     1861 aagatcaagc cggaaagccc ttcgtcggcc cagcgggcag gctgctcgac accgcattag
     1921 ttgcagccgg tatcgaccgc aaaaaagttt acataaccaa cgccgtaaaa catttcaagt
     1981 gggaaccgcg cggcaaacgc cgcatccaca aaaaacccaa cgccgcggaa atcgcggcat
     2041 gccgcccatg gctggattca gaaattgccg ccattagccc aaaggttatc gtctgtctgg
     2101 gcgccacagc agctcaagcc ttgctcggca aagattttcg cgtcacccag catcgcggcg
     2161 aattcatcga atccagcctc gctccctacg tcgccgccac cg
//
LOCUS       DATCBB010000290         1656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_463438, whole genome shotgun sequence.
ACCESSION   DATCBB010000290 DATCBB010000000
VERSION     DATCBB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463438"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /gene="nuoK"
                     /locus_tag="VII37_03265"
     CDS             <1..400
                     /gene="nuoK"
                     /locus_tag="VII37_03265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5068772.1"
                     /translation="AAVDVDTFYLVDRRSGFPCHISKKGPNVMLPLTPYLFLSFALLL
                     VGLIGVLTRRNLIIRLFSLEIILNATSINLAAFARLYGDASGQIFAMLTVVMLALELL
                     VALAIIAAVCSRWKRATPDDEKDLSSQLDY"
     gene            472..>1656
                     /locus_tag="VII37_03270"
     CDS             472..>1656
                     /locus_tag="VII37_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5068773.1"
                     /translation="MHGDGAMLLPEMQLLLFAIGTLGMDSWASNTSSEKPDGDPAFQQ
                     KYWNPAIALAGIAFSGLTLWMLRARIMQSGDLAGFHMTLVVDSYFIFFSALFLAATAL
                     VILLSVNYAGISPMRQGRYYALLLFACVSMMLMVSAVDLLVIFLVVEAASISAYFLAA
                     TPGLSNRTPAAAVKFLFSSALGSALVAYAFSLLYGLSGATNISQVAGALARRHNVAKV
                     IALSRQGGSQGLQMLQLLQSRLPEAMHWHTWTLEALPIAAFLLVSVGMILKFAVAPFQ
                     REDNPVNRETDFDIPNPVILYLCGAYTIAAVALLLRSMFTIFADSQNIWWVIVAALAI
                     AGISYGTFASLRHTNLERILVHLSLAHMSYVLLGVVAANEGAATGMTYYLFTYLFIAT
                     GAF"
BASE COUNT          309 a          416 c          480 g          451 t
ORIGIN      
        1 tgctgccgtc gacgttgata ctttttatct tgttgatcgg cggagcgggt ttccgtgcca
       61 catttcaaaa aagggcccga acgtgatgct accgctcaca ccctacctgt tcctgagttt
      121 cgcgctctta cttgttgggc ttataggagt tttgactcga cgcaacctga tcatcaggct
      181 gttttctctc gagatcattc ttaacgcgac gagtatcaat ttggcggctt ttgcccgttt
      241 atacggagac gcttcgggac aaatttttgc catgcttacg gttgtgatgc tggcgcttga
      301 actgctcgtg gcgctggcga tcattgctgc ggtttgcagc cgctggaagc gcgcaacacc
      361 ggacgacgag aaagacttat catcccaact tgattattaa ccgttagctt ctaagaccac
      421 atcgactacc gactgatgct taaccacatc cttaacgctt tccgtacgta tatgcacggc
      481 gatggcgcga tgctgctacc ggagatgcag ctacttctgt ttgccatcgg gaccttgggc
      541 atggattcgt gggcgtccaa tacgtccagc gaaaaaccgg atggtgatcc ggcttttcag
      601 cagaaatatt ggaacccggc aattgcgctg gcaggcatag cgttcagtgg attgacacta
      661 tggatgctgc gagcgcgaat tatgcagtcg ggcgatcttg ccggtttcca tatgacgctg
      721 gtggtggata gttacttcat atttttctcg gcgctgttcc tcgcggccac tgcgctggtg
      781 attctgcttt ctgtgaatta cgcggggatc tcaccaatgc gtcaggggcg gtattacgct
      841 ttattgttgt ttgcatgcgt gagcatgatg ctgatggtct ctgctgtaga tttgctggtg
      901 atatttctgg tggtggaggc cgcgtcaatt agcgcatatt ttctcgccgc aacgccgggc
      961 ctgtcgaacc gaactccagc ggcggcggtg aaattcctgt tttccagcgc gctgggctcg
     1021 gctcttgtgg cgtatgcgtt ttcgctgctg tatggattga gcggcgctac caatatcagc
     1081 caggttgcgg gagcgctggc gcggcgccac aacgttgcga aggtgatcgc gctcagccgt
     1141 caaggcgggt cacaaggctt gcagatgctg caactactgc aatcgcggct gcctgaagcg
     1201 atgcattggc atacttggac gctagaagct cttcccatag cggccttcct gctcgtgagc
     1261 gtgggcatga tattgaaatt tgcggtggcg ccattccagc gtgaagataa ccccgtgaat
     1321 agggagacag acttcgacat tccgaatccg gtgatcctgt atctttgcgg cgcgtatacc
     1381 attgcagcgg tggcgctctt gctgcgttcg atgttcacga tttttgcgga ttcccaaaac
     1441 atctggtggg tgattgtcgc ggcgttggcg atcgcgggga ttagttatgg gactttcgca
     1501 tctttgcgcc acacgaacct ggaacggatc ctggttcatt tgtcgctggc acacatgagt
     1561 tacgtgctgc tgggggtggt ggctgcgaac gagggcgcgg cgacggggat gacctactac
     1621 ttgttcactt atctattcat cgcgactgga gcgttc
//
LOCUS       DATCBB010000291         1225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_106896, whole genome shotgun sequence.
ACCESSION   DATCBB010000291 DATCBB010000000
VERSION     DATCBB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106896"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VII37_03275"
     CDS             <1..1084
                     /locus_tag="VII37_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5068774.1"
                     /translation="TSVFELSSGNARKIEEPDGIIFLKSLKDAGKEVERNSGASLIDL
                     GDGVVCCEFHSKMNAIGADLISMLHKGLKRLETNFDAMVIANQAVNFSVGANLMLVLM
                     GAQEQEWDELHLAVKQFQNANLAVKYAPKPVVTAPQGMALGGGCEVSLHGARIQAAAE
                     AYMGLVEVGVGLIPGGGGTKEMMIRANEHAGGGEDLDLFHAMKPAFETIAMAKVGTSA
                     EECRDLGFLRREDGVSMNRDRLVGDAKEVALSLVRNGYRPKAANWQEGAQTTQIKVLG
                     EQFLSGAKLVIHMMQRGGYITDYDAHVGRKLGNILAGGALSSPQFVSEQYVLDLEREA
                     FVSLCGEKKTQERIAHTLKTGKPLRN"
BASE COUNT          308 a          321 c          350 g          246 t
ORIGIN      
        1 cacctccgta ttcgaattaa gtagcggcaa cgccagaaaa atcgaagagc cggacggcat
       61 catttttctc aagtccctga aagacgccgg aaaagaggtg gaacgaaact ccggcgccag
      121 cctgatcgat ttgggcgatg gtgtcgtatg ctgcgaattt cactccaaga tgaacgccat
      181 tggcgccgat ttgatcagca tgctgcacaa aggattaaag cgactcgaaa ccaatttcga
      241 cgccatggtt atcgccaatc aagcggtgaa tttttccgta ggcgcgaact tgatgctcgt
      301 attgatgggc gcgcaggaac aggaatggga cgagctgcac ctggccgtca aacaattcca
      361 gaatgccaac ctggccgtta aatatgcgcc aaagccggtg gtcaccgctc cgcaaggtat
      421 ggcattaggc ggcggctgcg aagtaagtct gcacggtgca cgaattcaag ccgctgcgga
      481 agcttacatg ggcttggtgg aagtaggagt cggcttgatt cccggcggcg gtggcaccaa
      541 agaaatgatg attcgcgcca acgaacacgc tggcggcggc gaagacctgg atttattcca
      601 cgccatgaag ccagccttcg aaactattgc gatggcgaaa gtcggcacca gcgcggaaga
      661 gtgtcgcgac ctgggtttct tgcgacgcga agatggcgtc tccatgaatc gcgaccgctt
      721 ggttggcgac gccaaagaag ttgctctgtc actggtgcgc aacggctacc gacccaaagc
      781 cgcgaactgg caagagggtg cgcaaaccac gcagatcaaa gtgctcggcg aacaatttct
      841 gtcgggcgcc aagctggtca ttcacatgat gcagcgcggc ggctatataa ccgattacga
      901 tgcccacgtg gggcgcaagc taggaaatat tctcgccggt ggcgcgttga gctcaccaca
      961 gtttgtaagc gaacaatacg ttttagactt agaacgcgaa gctttcgtgt ctctatgcgg
     1021 cgaaaagaaa acccaggaac gcatcgccca caccctaaaa accggcaaac cgctgcgaaa
     1081 ttaaacgaat ttaatggtaa gttataggtg attcgctgcc gatcagcgga gcgctatgaa
     1141 atttccatcc aaactcatcg tcgttctgct cgtcgggctt ttcgctggcc cctatgcgcg
     1201 cgggcaagcc gtgagggttg cagcg
//
LOCUS       DATCBB010000292         1291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_333379, whole genome shotgun sequence.
ACCESSION   DATCBB010000292 DATCBB010000000
VERSION     DATCBB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333379"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /gene="xseA"
                     /locus_tag="VII37_03280"
     CDS             <1..1291
                     /gene="xseA"
                     /locus_tag="VII37_03280"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY5068775.1"
                     /translation="HIEREYGDTWVEGEISNFRAHDSGHLYFTLKDQNSQLSAVMFRS
                     QAKLLRFRPENGMQVVARGRVTIYEDRGQLQLSAEYLEPKGAGALQIAFEQLKAKLEA
                     EGLFDAARKKSIPSLPRRIGIVTSPQGAALRDILNILQRRHHTANILIFPSQVQGEAA
                     PPEVMAGIRYFNKACNVDVIVVARGGGSAEDLAAFNHEGLARAIASSEIPVISAVGHE
                     TDFTIIDFVADLRAPTPSAAAELVIRSRQDIEEQSEELRLRLSRAIRYRLLMGRQALT
                     ELAQHGAFARMMDFINQRQQKLDDLTYRLEKSERKLLEQNRRRWEAVAAAVRHYDVRR
                     VLAGIRKEMDSLAAAMIAATRNQLLQQKARLDRITRQIEALSPLAILDRGYALVFESS
                     GKLLKDASMVAPGDEISARLSRGTVAATVKQTRPNQP"
BASE COUNT          281 a          355 c          384 g          271 t
ORIGIN      
        1 ccatattgaa cgcgaatacg gagacacatg ggtcgagggc gaaatttcaa atttccgggc
       61 gcacgactct ggacatctct acttcacctt aaaagatcag aattcccagc ttagcgccgt
      121 gatgtttcgc tcgcaagcca agctgttgag gtttcgcccc gagaatggaa tgcaagtcgt
      181 ggcgcgtgga cgggtcacga tttacgaaga ccgtggccag ttgcaattgt cagcggaata
      241 ccttgagccc aagggagcgg gcgcgcttca gattgccttc gagcagctca aggccaaact
      301 ggaagcggaa ggactattcg atgccgcgcg caagaagtcg attccgtcat tgccccggcg
      361 gatcgggatc gtaacttctc cgcagggtgc ggctttgcgg gatattttga acatcctgca
      421 gcggcggcac cacacggcta atattttgat ttttccttcg caggtgcagg gagaagctgc
      481 gccgccggaa gtgatggccg gcatccggta cttcaataag gcttgcaatg tcgacgtgat
      541 cgttgtggcg cgtggaggcg gatcggccga ggatctagct gcgtttaacc acgaaggtct
      601 ggcccgcgcg atcgcctcgt ctgagattcc ggtcatctct gcggtcggac acgaaaccga
      661 tttcacaatc attgattttg ttgccgatct tcgcgctccc accccttcag ccgccgcgga
      721 actagtgatt cgatcacggc aagatattga ggaacagtcg gaggagctgc gtttgcggct
      781 ttcccgcgcc atccggtacc ggttgctcat gggcaggcag gcgctcacag aattagcgca
      841 acacggggcg tttgcgcgga tgatggattt catcaatcag cgccagcaaa aattggacga
      901 cctcacatat cgtctggaga aatccgaacg caaattgctg gagcaaaatc ggcggcggtg
      961 ggaagctgtt gcggcggcgg tccgccacta cgatgtccgg cgcgtgctgg cgggcatccg
     1021 taaagagatg gattccctgg cggcggccat gattgccgca acccgcaacc aattgcttca
     1081 gcaaaaagcg cgcctggatc gaatcacgcg gcaaattgaa gcgctttcac cgctggccat
     1141 tctcgatcgc gggtacgccc tggtgtttga atcttccggc aagctgctaa aagacgcatc
     1201 catggtggcg ccgggggatg aaatttccgc tcgactttcg cggggaactg tagcggcgac
     1261 tgtcaagcaa acccgcccga accaacccta g
//
LOCUS       DATCBB010000293         6483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_301364, whole genome shotgun sequence.
ACCESSION   DATCBB010000293 DATCBB010000000
VERSION     DATCBB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301364"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VII37_03285"
     CDS             complement(<1..498)
                     /locus_tag="VII37_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY5068776.1"
                     /translation="MPEGDTIYRAARTLNLALAGQTVTSFESVLPHLSRVEVDHGVVG
                     RTVEKVEAQGKWLLMHFSGGLILLSHMLMSGSWHIYRPGETWKRRRYDMRVVIATPKI
                     LAVAFNVPIAEFHTAESLARREGFRSVGPSTLAEDFDEPAVIARLRARPDLEIGVSLL
                     TQSLLS"
     gene            complement(500..5206)
                     /locus_tag="VII37_03290"
     CDS             complement(500..5206)
                     /locus_tag="VII37_03290"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY5068777.1"
                     /translation="MSDPNNLSSPGSVALDAALFQAPVRDWFEAVFAAPTRPQVQGWP
                     AIARGESTLILAPTGTGKTLAAFLWCINRLMFSPVPEKLQRCRVLYISPIKALAVDVE
                     RNLRAPLVGIAQAAKRMGVQFHEPQVAIRTGDTPPAERARFLRHPADILITTPESLYL
                     LLTSNARESLRSIETVVIDEIHALVPSKRGSHLALSLERLEHLCGRRLQRIGLSATQR
                     PLEEVARYLGGAESPSAKALPSATPIKEPEILTESDAETSAVVYRPVTIIDASEPKRL
                     DLRVEIPVEDMTRLDEVDRLPSGPASQGPTRPSIWSAIHPKLLELVRSHRSTLIFVNS
                     RRLAERISAAVNELAGEILVRAHHGSVAVAQRKEIEDRLKLGTLRGLVATSSLELGID
                     MGAIDLVVQIESPSSVASGMQRVGRASHHVGAVSAAVIYPKYRADLVACAAITRAMYD
                     GNVESVHYPRNPLDVLAQQLVAMVSVQPWDVANIFKVVQSAAPYVGLTLGAFEGVLDM
                     LSGRYPSEEFTELRPRLTWDRLANKLTTRQGARQVSIINGGTIPDRGLYGVFLAGATK
                     GARVGELDEEMVFESRPGDTIILGASTWRIENISHDRVLVSPAPGEPGKMPFWHGDTA
                     GRPAEFGEKIGRMTRELLALPRSVAFSKLVDQHSLDVNAAENLLRYLQDQVAATQRVP
                     SDEDVVVEICRDELGDRRICVLTPFGSRVHAPWCMAVTAKLRAERGLEVESMWSDDGF
                     VLRLPDSDAPVETEDFVLSPAEFKELVLRQLGSTSLFAAKFREAASRALLLPKRRPGM
                     RAPLWQQRKRAADLLAVASRFSSFPIILETYRECIRDVFDLAAAAAILSKIQRGEIRL
                     TRVESDKPSPFAGALLFSYIANYIYEGDAPLAERRAQALSIDQSQLEELLGDTDLREL
                     LDSSALDEVEAKLQALEPDYQARHADGLHDLLLKLGDLSDPEIAARSASPEVAATAAE
                     LTNTRRAVRVRIAGEARTIPAEYAGRYRDALGTPLPPGLAEAFLQAPADPLTEIIRRY
                     ARTHGPFTTADIATRYHLQTPAVEAVLTTLNSQGKLLEGEFRPNGHHREWCDPEVLRQ
                     LRRKSLARLRREIEPVEQSTFVRFSTRWQGVTVPRRGLNALLDTIEALQGAALLASEL
                     ETEILPARIADYEPGNLDTLMAAGEVTWIGVEQIGDRNGRVALYLTEALPLLRLPEAA
                     AVTGPNVPQQTPHSERAEKVVEFLARQGASFFSEIHNSCGGGFPGDTVDALWQLVWAG
                     RITNDTFYPLRQLLRPDDRKRKHEAFDDERPGSPGYLRRLRSRTSTGQAQGRWSLIGQ
                     RITVAPTPTQWSANISQQILQRNGIVMRETAIAENIPGGYNTIYPALKTMEDSGWVRR
                     GMFVAGLGAAQFAMPAAVDMLRSLRVDPGTPEVLHLAATDPANPYGTLISWPKIEGES
                     DETLSHHSMARTAGASVILINGQLAAFLRRRNPALRIFLPESEPGRSLFARELAKKLA
                     ELAIRRLNRRQGLLIGEINNASAREHFLAPYLEDARFVNTVLGYQMRRTKPLIVSSDP
                     PADAEDEDTPEIQENA"
     gene            complement(5265..6059)
                     /locus_tag="VII37_03295"
     CDS             complement(5265..6059)
                     /locus_tag="VII37_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY5068778.1"
                     /translation="MEDNILDLAAQVSFYFVLSLVPFLIVLAAIVGWLPSTTLWQSFA
                     QWITDYFPNRSRQAVFATILDLTRGYTGFLSFGLLAAVWSASSGFMGLMEALSIACCG
                     RDTRSYWKKRAIATVTTLVASLFCLTAFGLWTLGHWATREIFSDFKFVGTIHFRWSVI
                     AWWLGTLLLMFVALTLINHFLPDVKRPWRWFTIGNIFSVTGILITSVGFNLFLQYSPT
                     VPQVYTVLAGFVILMTWIYVANVIILIGTELDMAITLLHKQREKAA"
BASE COUNT         1495 a         1714 c         1968 g         1306 t
ORIGIN      
        1 cgaaagcaac gattgggtaa gcaggctaac gccgatttcc aagtccgggc gggcgcgcag
       61 tctggcaatc acagcgggtt cgtcgaagtc ttccgcaaga gttgagggac cgacggacct
      121 aaatccttcg cgtcgagcaa gcgattccgc ggtgtggaat tccgcgatag gaacgttaaa
      181 ggccaccgcc agaattttgg gggtggcaat tacgacgcgc atatcgtacc gccggcgttt
      241 ccatgtttct cctgggcggt agatatgcca gctaccgctc atcagcatgt gtgaaagaag
      301 aatgaggccg cccgaaaaat gcatgagcag ccacttccct tgcgcttcga ctttttctac
      361 ggttcttccc acaacgccgt gatccacttc cacgcgggag agatgcggca acactgattc
      421 gaaagaggtg acggtttggc ctgcgagtgc aaggttcaag gtgcgagccg cacgataaat
      481 agtgtcgcct tcaggcatct caagcgtttt cctgaatttc tggagtgtct tcgtcttcgg
      541 catccgcggg cgggtcggaa ctaacaatga gcggcttggt gcggcgcatt tgatagccga
      601 gaacggtatt cacgaagcga gcgtcttcta aatagggagc gagaaaatgt tcgcgtgctg
      661 aagcgttatt gatttcgccg atcaacagac cttgtcggcg attgaggcga cggatagcca
      721 gttccgccaa tttctttgcg agttcgcgcg cgaagaggct gcgtccaggc tctgactcgg
      781 gaagaaaaat ccgtaaggcc ggattgcggc gccgcaaaaa agcggcgagc tgcccgttta
      841 tcaaaatgac ggatgcgccg gcggttcggg ccatggaatg gtgactgaga gtttcatcgg
      901 attccccttc aatcttcggc caggaaatta aagtgccgta gggattggcc ggatcggtgg
      961 ctgcaagatg caagacttcc ggagttccag gatccactcg caggctgcgg agcatatcca
     1021 ctgcggcagg catggcgaat tgtgcggcac caagcccggc aacaaacatg ccgcggcgga
     1081 cccagccgct gtcttccata gtttttagcg ccgggtaaat ggtgttgtag ccgcccggaa
     1141 tgttttctgc gatggcggtc tcgcgcatca cgataccgtt gcgctgcagg atttgctgag
     1201 agatatttgc gctccattgc gtgggggtgg gagcgacggt gatgcgctgg ccgataagcg
     1261 accagcggcc ttgtgcttgc cccgtggaag ttcgcgaacg cagccgccgc aaatatccag
     1321 gagagccagg acgctcgtca tcaaacgcct catgtttgcg tttgcgatca tctggccgca
     1381 aaagctggcg aagcggataa aaagtgtcgt tggtaattcg tccggcccac actagttgcc
     1441 acaatgcgtc aacggtgtcg ccgggaaacc ctccaccgca actgttgtga atttcggaaa
     1501 agaaggaagc cccttgacga gcgagaaatt cgacaacttt ttcagcgcgt tccgagtggg
     1561 gagtctgctg cggcacattt gggccggtta ctgccgcagc ttcgggtaaa cgcaaaagcg
     1621 gtaaagcttc ggtcagatac aacgcgaccc tgccgttgcg gtcgccaatt tgttcgacgc
     1681 ctatccaagt gacttccccg gcggccatga gcgtgtcgag gttgccgggt tcgtagtcgg
     1741 cgatacgcgc aggcaaaatt tcggtttcca actctgaagc gaggagcgcc gcgccctgca
     1801 gagcttcgat ggtgtcgagg agcgcgttca acccgcgacg cgggacggtg acgccttgcc
     1861 atcgcgtcga gaatctaacg aaggtagatt gttcgacggg ctcgatctcg cggcgcaatc
     1921 gcgcaagact tttcctgcga agttgtcgca acacttcggg atcgcaccat tcgcggtgat
     1981 ggccgttggg cctgaactcg ccttccaata atttgccttg agaattcaag gtggtcagca
     2041 cggcttccac cgcaggtgtt tgcaagtgat agcgcgttgc gatatcggca gtcgtgaatg
     2101 ggccgtgagt gcgcgcgtaa cggcgaatta tttccgttaa agggtcggcc ggggcttgaa
     2161 gaaacgcctc ggcgaggccg ggaggcagcg gcgtgcccag agcatcgcgg tatcggcctg
     2221 cgtattcggc ggggatggtg cgggcttcgc cggcaatgcg cacgcgcacg gcacggcgag
     2281 tattggtcaa ttccgcagcg gtggcggcga cttccggact ggctgagcgg gcggcaattt
     2341 cgggatcgga taaatcgcca agcttcaaca acaagtcgtg gagtccgtcg gcgtgacgcg
     2401 cctgataatc cggttccaga gcctgaagtt ttgcttccac ttcgtcgagg gcggatgagt
     2461 caagcaactc gcgcaaatct gtatcgccga gtaactcttc gagttgtgat tgatcgatgg
     2521 aaagggcctg agcgcggcgc tcggccagcg gcgcatctcc ttcataaatg taattcgcga
     2581 tgtaagaaaa gagcagcgca ccggcgaatg gggacggctt gtcggattcc acgcgcgtta
     2641 atcggatttc gccacgctga atcttgctga gaattgcggc agcagcagcc aaatcaaata
     2701 catcgcgaat gcattcacgg taggtttcca gaattatggg gaacgaagag aagcgcgaag
     2761 ctacggccaa taaatcagca gcacgcttgc gctgttgcca caacggcgcg cgcatgccgg
     2821 gccgacgctt gggcaacaac agcgcgcgag aggccgcttc acggaatttg gcggcaaata
     2881 aggaagtgga tcccagttgg cgcaggacca actctttaaa ttccgcagga gacaatacga
     2941 aatcttcggt ttctacaggg gcgtcactgt ccggaaggcg caaaacgaaa ccgtcgtcgg
     3001 accacatgga ttcgacttcc agtccacgct cggcgcgaag tttggcggtt acggccatgc
     3061 accatggggc atgaactcgg ctgccaaaag gggtcagcac gcagatgcga cgatcgccga
     3121 gctcgtcgcg gcaaatttcc acgactacgt cctcatcgct aggcactcgt tgcgtagccg
     3181 cgacttgatc ttgcagatag cgaagaaggt tttcggcggc gttaacatcc aggctgtgtt
     3241 gatcaacgag tttcgagaac gccacggagc gcggcaaggc aagcaattcg cgagtcatgc
     3301 gtcctatttt ttcgccgaat tcagctggac ggccagcggt atcgccatgc cagaagggca
     3361 ttttgccggg ctcgccgggc gcgggagaaa cgaggacgcg atcatgagaa atgttctcga
     3421 tgcgccaagt ggaggcgccg aggatgatgg tgtcgccggg cctgctttcg aagaccattt
     3481 cttcgtcaag ttcgccgacg cgcgcgcctt tggtggcacc ggccaggaat acgccgtaca
     3541 agccacgatc tggaatggtt ccgccgttga tgatggagac ctgacgcgcg ccctggcggg
     3601 tggttaattt gttggcaagg cgatcccagg taagtcgcgg gcgaagttcc gtgaattctt
     3661 cggagggata gcggcctgaa agcatgtcca gcacgccttc gaacgcgccc aaggtcaggc
     3721 cgacataagg ggcggcggat tgcacaacct taaaaatgtt ggcgacatcc cagggctgga
     3781 ccgagaccat ggcaacgagt tgctgcgcga gcacgtcgag tgggttgcgg gggtagtgca
     3841 cggactcaac gttgccgtca tacatggcac gagtgatggc cgcgcaggcg acgaggtcgg
     3901 cgcgatactt gggataaatg acggcggcgc taacagcacc tacgtggtgg ctggcgcgcc
     3961 cgaccctttg catgccgctg gcgacggatg atggcgattc gatctgtacc accagatcga
     4021 tggcgcccat atcgatgcca agttccagag aggaggtagc taccaggccg cgcaaagttc
     4081 caagcttcag gcggtcttca atttctttgc gctgtgctac ggccacgctg ccgtgatgag
     4141 cgcgcaccag aatttcgccg gctaattcat ttactgcggc ggaaattctt tccgcgaggc
     4201 gcctgctatt cacaaaaatc agcgtggagc gatgagagcg caccaactca aggagcttag
     4261 ggtggatggc actccagatg gagggacggg tcgggccttg tgacgccggt ccactcggca
     4321 aacgatcgac ctcgtcgagg cgggtcatat cctcaacggg aatttccacg cggagatcga
     4381 gacgctttgg ttcactggca tcgattatcg tgacgggacg atagacgacc gcggatgtct
     4441 ctgcgtctga ctcagtcaag atttcgggtt ctttaatcgg ggtggcagac ggcaatgctt
     4501 ttgcgcttgg gctttctgcg ccacccaagt agcgagcaac ttcttccaga ggacgctgcg
     4561 tggcggagag gccgatccgt tgtaaccgcc gcccacacaa atgttcgagg cgttccagcg
     4621 acaacgccag atgggatccg cgcttgctgg gcaccagcgc atgaatctca tcgatgacca
     4681 ccgtctcaat ggagcgcagt gattcacgcg cgttggaagt taacaacaga taaagagact
     4741 cgggcgttgt aattaggatg tccgccggat ggcgcaggaa gcgtgcgcgc tcggcgggcg
     4801 gggtgtcgcc cgttcgaatg gcaacttgcg gctcgtgaaa ttgaactccc atgcgcttcg
     4861 cggcttgcgc aataccgacc agcggagcgc gtaaattgcg ttccacgtca accgcgagcg
     4921 ctttgatcgg tgaaatgtaa aggacacggc aacgctgcag cttttccggg accggcgaga
     4981 acatgagccg gttgatgcac cacaggaagg ccgctagagt cttccccgtt ccggtgggcg
     5041 caaggattaa ggtcgattcg ccgcgtgcga ttgccggcca gccctgcacc tgaggtctgg
     5101 tgggagcggc gaaaacagcc tcgaaccagt cacgaacagg ggcttgaaac agagcagcat
     5161 ccagggccac cgaaccgggc gaagaaagat tgttgggatc ggacatactg agtctttatt
     5221 tagatgcctg aagggcgaaa tttgttggga gattaggcga agtcttaagc ggccttctct
     5281 ctttgcttat gtagcagcgt aatggccatg tccagttccg tgccaatcaa aataatcaca
     5341 ttggctacgt aaatccaggt catcaaaatc acaaagccgg ccagtacggt atacacctga
     5401 ggaacggtgg ggctgtactg caagaacaga ttgaagccca ccgacgtgat caagatgccg
     5461 gttaccgaaa agatgttgcc aatagtgaac caccgccagg gtcgcttcac atcgggaaga
     5521 aaatgattga ttaaagttag cgccacgaac atgagcaaca atgtgcctag ccaccaggca
     5581 atcacgctcc atcgaaaatg aatggtgccg acgaatttga agtcactaaa tatttcgcgg
     5641 gtggcccagt ggccgagagt ccaaagacca aaggccgtca ggcaaaacaa agaggccacc
     5701 agcgtcgtca ctgtagcgat ggcacgcttt ttccaatagc tacgagtatc tctgccgcag
     5761 caggcgatgc ttaacgcctc catcaaaccc atgaatcccg aagatgcact ccacaccgcg
     5821 gccaacaagc cgaaggaaag aaatccggtg tacccccggg tgagatcgag aatggtggca
     5881 aaaacagctt gccgggaacg attaggaaaa taatcggtga tccattgtgc gaacgattgc
     5941 cataacgttg tggatggaag ccatcccacg atggcggcaa gaacgatcag gaagggtacc
     6001 agcgacagca caaagtaaaa cgagacctgg gccgcaagat ccaggatatt atcttccatc
     6061 accctggaaa atgtgagttc gatgacttcg gagaggccgc gtcgtaacgc ggtgctgcgc
     6121 ctgaaaaaac ggtaccaccg gtggcccgcg gaccttctag ctgtcggcgc agctccggat
     6181 tggagctcgg gggaatgcaa tcgacggctc gagctagagt gtcgcatctt ttaaaactgc
     6241 gggtcgctga tgcagtgttg cgcacacgac aacatacaag cgccagaaaa gatgataatg
     6301 cagaagaagg gataaaagcc caggaatcgg acagacgagc cccatacgca tttagaggtt
     6361 gttatcgaaa ttcgatcaca ggtatacttc ttgtgcttta tctgacggtc cccggcagtg
     6421 tattgaactg aaacgcctcc cccagcaggc gtcgcaagat cgcaagacca aactaatttt
     6481 ata
//
LOCUS       DATCBB010000294         4771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_203880, whole genome shotgun sequence.
ACCESSION   DATCBB010000294 DATCBB010000000
VERSION     DATCBB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203880"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /gene="topA"
                     /locus_tag="VII37_03300"
     CDS             complement(<1..1812)
                     /gene="topA"
                     /locus_tag="VII37_03300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY5068779.1"
                     /translation="MARSLVIVESPAKAKTINKYLGREYTVKASLGHVLDLPKKTLGI
                     LLPGSENGNKKKKKRKTKAKAKDGEKVVEEKKPPKPVVVTAENIFEPTLEVIPGKVKV
                     IADLRKAAEIAPNVYLATDPDREGEAISQHLLDTLATKKSDRAKYRRVMFNEITPKAI
                     KAAFANAGDVNADLVDAQQARRVLDRLVGYKISPILWNKVRRGLSAGRVQTVALRLIV
                     EREFLIREFVPQEYWSIHAMLDAGEPPLFEAKLFKHKGEDVEVSNQETADKIVAVVSD
                     AKWEVANVTQKEKRRNPPPPFTTSKLQQAAYNRLRYTAKRTMSLAQKLYEGVELGEEG
                     SVALITYMRTDSVNVSADALAQVREMIPERFGSNYLPEKPNYYKSKKDAQEAHEAVRP
                     TDAMRAPEDVRKYLEDDLFKLYQLIWQRFVASQMLPAIFDQTTIDIAAKDYTFRANGS
                     VQKFDGYLRVYQTPAAIADREDDEKADEGEGNALPRVTEGQILCLDKIRPDQHFTEPP
                     PRYNDATLVKDLEEKGIGRPSTYASIISTLVDREYVKKDQGRFTPTMLGERVSVLLIR
                     SFEDIFDVTFTARLEDELDEIEEGKLGWRAAVGEFWDK"
     gene            complement(2110..3099)
                     /gene="dprA"
                     /locus_tag="VII37_03305"
     CDS             complement(2110..3099)
                     /gene="dprA"
                     /locus_tag="VII37_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY5068780.1"
                     /translation="MELDASLSWLALALTLGLACRLCARLLRKIGSPDRIFHASLTEL
                     ESCSLPARVAQTIYNKDAFKRAEKELAGIRNIPGCRLLNWTEPEYPQNLLQIYDPPIL
                     FYLRGDPQILNMPCLSIVGTRRPTLYGSQMAERLGRDLAARGLVILSGMARGIDAIGH
                     QGALASNGRAIGVLGTGIDVCYPKENKKLYEKVLERGAILSEFPLGTHHAPENFPIRN
                     RIVAGMPLGVIVIEGAEFSGSLITARLAMEFGREVFGVPGNVTQTVSYAPNMLIKLGA
                     KLVVNAEDVIEELPTPVRARAGKSRTPRSGAAKFAGFRCFGSLGKETLRTTGS"
     gene            complement(3165..>4771)
                     /locus_tag="VII37_03310"
     CDS             complement(3165..>4771)
                     /locus_tag="VII37_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5068781.1"
                     /translation="DAGDAGEFFTLYGHLDTESLVGLKIGQRVARGETFARIGTSEEN
                     GGWAPHLHFQIILDLLDLDANFPGVAYASQRAVWTCLSPDPNLLIGIPKNRFPARDPT
                     HQETLTRRRDVLGKNVSISYEKPLKIVRGWMQHLYDDTGRAFLDFYNNVPLVGHSHPR
                     VVRAVQKQIALLNTNTRYLHDVANRYAQSLTQHLPDPLRVCFFVNSGSEANELALRLA
                     RTHTQREDIIVLEHAYHGNTSTLIDISPYKFDGPGGSGRKPWVHIACMPDDYRGKYRR
                     EDAQAGTKYAAHVGEILDAMHRQGRAPAAYLAETLPSVGGQIVFPPNYLEQVYTRVRA
                     AGGLCIADEVQVGFGRLGTHFWGFETQNVIPDIVVLGKPIGNGFPLAAVITTPGIARS
                     FDNGMEFFSTFGGNPVACAAGLAVLRVVEEEKLQERAFRVGGYLKESLRALQKQYPII
                     GDVRGSGLFLGVDLVLDTVTRSAATAQAAYVVNRLRECGILTGTDGPFNNVIKLRPPL
                     IISERDVDIFTETLRTVLAEDSAQPA"
BASE COUNT         1021 a         1308 c         1290 g         1152 t
ORIGIN      
        1 cttatcccag aattcgccga cagccgcgcg ccaaccgagc ttaccctctt cgatttcgtc
       61 cagttcatct tccaggcgtg cagtaaaagt gacatcaaaa atgtcttcga aactgcgaat
      121 cagcaaaaca ctgacgcgct cccccagcat cgtgggtgta aagcgacctt ggtctttctt
      181 cacgtattcg cgatccacca acgtggaaat gatggaagcg tatgtggacg gccggccgat
      241 acccttttct tccagatctt tcaccagcgt ggcgtcgttg taacgcggcg gaggctcagt
      301 gaaatgttgg tccgggcgaa tcttgtcgag gcataggatt tgcccttcgg ttacgcgagg
      361 caaggcattg ccttcacctt cgtccgcctt ctcgtcgtct tcgcggtccg cgatagctgc
      421 cggcgtttga taaacgcgca agtaaccgtc aaatttttgg accgagccgt tggcgcgaaa
      481 agtgtaatct ttggcggcaa tatcaatcgt agtttgatcg aaaatcgcag gcagcatttg
      541 cgacgcgaca aagcgctgcc agatcaattg atacagcttg aagagatcgt cttccagata
      601 tttgcgaaca tcttcaggcg cgcgcatcgc gtccgtgggg cgaaccgctt cgtgagcttc
      661 ttgcgcgtct tttttggatt tgtagtaatt cggcttctcg ggcagataat tcgaaccaaa
      721 gcgctcggga atcatttccc gcacttgcgc gagagcatcc gccgaaacgt tcacagaatc
      781 agtacgcatg taagtaatca gcgcgactga cccctcttcg cccagctcaa ctccttcgta
      841 aagcttttgc gccagcgaca tggtgcgttt ggcggtataa cgcaagcggt tgtaggcggc
      901 ctgctgcagc ttggaagtcg tgaatggcgg aggtgggttg cggcgctttt ctttctgcgt
      961 gacgttggcc acttcccact tggcgtcact gacgacggca acgattttgt cggccgtttc
     1021 ttgattggaa acttcaacgt cctcgccttt gtgtttgaaa agcttggctt cgaacagtgg
     1081 cggctcgccg gcgtccagca ttgcgtggat gctccaatac tcctgcggca cgaattcgcg
     1141 aatcagaaac tcgcgttcca cgatcagtcg cagcgccacc gtttgcactc ggccggcaga
     1201 caggccgcgg cgcactttat tccaaagtat gggagaaatt ttgtagccca ccaggcggtc
     1261 aagaacacgt cgtgcctgtt gtgcgtccac caggtcggca ttcacgtcac cagcattggc
     1321 gaaagcggcc ttgatagctt tcggcgtaat ttcattgaac atcacgcgac gatacttggc
     1381 gcgatccgac ttcttggtag ccagcgtatc cagaagatgc tgcgagatgg cctcgccttc
     1441 tcggtcaggg tcagtggcca ggtacacgtt gggagcgatt tcggccgcct tgcgtaggtc
     1501 cgcgatcacc ttgaccttgc caggaatgac ttccagcgta ggttcaaaga tattttccgc
     1561 ggtaactacg accggctttg gcggcttctt ctcttcgacg accttctcgc catctttcgc
     1621 ttttgccttg gtcttgcgtt ttttcttttt cttgtttccg ttttcactgc cgggcaaaag
     1681 gatgcccaac gtctttttag ggagatccaa gacgtggccc agggaggcct tgacggtata
     1741 ttcccgcccc aaatacttat tgatcgtttt cgcctttgcc ggcgattcca caattacgag
     1801 cgaacgagcc atgttgcctt tgtaactccg taatcttccg caggaagggt cgactgcagg
     1861 ggagtgtgtc cttctcgcta ccttagcaga tgcggagatt gtgaatccgg gtgctttttg
     1921 cgcggggcgc gtgcaccgaa ttcctaatgt gattcgacca ttaaatccaa ctacaacata
     1981 actttactga attgtttgcc aggcaattgc ctaaccacgc ctttcatctc cagatcaaaa
     2041 agcgtagcca gaacctcgga agagttcaag ccagagtttt cgacaagttc atcaatgtgc
     2101 cgcgtttcct caactaccag tagttcgtag agtttctttt ccgaggctcc caaagcagcg
     2161 aaagccagca aatttcgctg ctccgcttcg gggcgttcgg cttttaccag cgcgggcgcg
     2221 caccggcgtg ggcagttctt cgattacatc ctcggcgtta acgaccagct tggcgcctag
     2281 tttgatcagc atatttggcg cgtagctaac cgtttgcgtg acgtttcccg gaacaccaaa
     2341 aacttctcgg ccaaattcca tggccaggcg cgcggttatt aaggacccgc taaactcggc
     2401 tccctcgatt acgatcaccc cgaggggcat gcctgccacg atgcgattcc tgatcgggaa
     2461 attctcgggc gcatggtgcg ttccaagggg gaactcgcta aggatggccc cgcgctccaa
     2521 aactttttcg tagagctttt tattttcttt cgggtaacaa acgtcgatgc ccgtgcccag
     2581 cacaccgatt gcgcggccgt ttgacgccaa ggccccctga tggccgatgg catcgatacc
     2641 gcgtgccatg ccgctgagga ttaccaggcc tcgtgctgcc aggtcgcggc cgaggcgttc
     2701 cgccatttgt gaaccgtaaa gcgtgggccg tctcgtaccg acgatgctga ggcacggcat
     2761 gttaaggatt tgcggatcgc cgcgcaggta aaagaggatc ggtgggtcat aaatctgcaa
     2821 caggttttga ggatattccg gttcggtcca attcagcagg cggcaaccgg gaatattgcg
     2881 gatgcctgcc agttcttttt ccgcgcgttt gaaagcatcc ttattataga tagtctgcgc
     2941 cacacgcgcc ggcaggctgc aagactccag ttccgtgaga gacgcgtgaa atatgcgatc
     3001 aggcgatcca atcttgcgca acaaacgggc acacaagcgg cacgccagcc cgagagtcaa
     3061 cgccagcgcg agccaggaga gggaagcgtc tagctccatc gcgtgttgag cgtatgcgct
     3121 tgattaccta tttgtcaact atcaataata ttatataggt ggggttatgc aggctgggcg
     3181 gaatcttcgg ccaacacggt cctcagtgtt tccgtgaaaa tatcgacgtc gcgttcggaa
     3241 attatgagtg gtggccggag cttaatgaca ttgttaaacg ggccatctgt tcctgtgagg
     3301 atgccgcatt cgcgaaggcg gttcactaca tatgccgctt gcgcggttgc tgcggaacga
     3361 gtaacggtat ccaaaactag atcgacaccc aggaataaac ccgagccacg cacatctcca
     3421 atgatcggat attgcttttg aagtgcgcga agagattcct tgagataacc gcccactcga
     3481 aacgcgcgtt cctgtaactt ttcttcttcc acgactcgca gcactgccag tcccgcggca
     3541 caggcgacgg gattgcctcc aaaggtgctg aagaattcca tgccattgtc gaatgaccgc
     3601 gcgattcccg gcgtagtgat tactgctgcc aatggaaagc cgttgccgat gggctttccc
     3661 agaacaacta tgtcaggaat cacattttgc gtttcaaatc cccagaagtg cgtacccaat
     3721 cggccgaaac caacttgcac ttcgtcggcg atgcacaggc ctcccgcagc gcgaacgcgc
     3781 gtgtatacct gctctaaata atttggcgga aaaacaattt ggcccccgac gcttggaaga
     3841 gtctccgcga gatacgcggc cggcgcacgc ccttgcctgt gcatggcgtc cagaatttcg
     3901 ccgacatgtg ccgcgtattt cgttcccgct tgcgcatcct cgcggcggta ttttccgcga
     3961 tagtcatcgg gcatgcacgc gatgtgaacc cacggcttcc ggccactgcc gccaggcccg
     4021 tcaaacttat aggggctgat gtcgatcagt gttgacgtat ttccatggta agcgtgttcc
     4081 agaacgatga tgtcctcacg ttgtgtgtga gtgcgcgcga gccggagcgc gagctcattg
     4141 gcttcgctgc cggaattgac gaagaaacac actcgtaacg gatcgggcag atgttgcgtg
     4201 aggctttgag catagcgatt tgcaacgtcg tgcaagtagc gcgtattggt attgagtaac
     4261 gcaatttgtt tttggacagc tcgcaccaca cgaggatggc tgtggcccac cagcggtaca
     4321 ttgttgtaaa aatctaaaaa cgcgcgcccg gtgtcatcgt aaagatgttg catccagccg
     4381 cggacgattt tcagcggctt ctcgtacgag atgctcacat ttttgcccaa cacgtcacgc
     4441 cgtctagtca gtgtttcctg atgggtgggg tcccgggccg gaaaacggtt cttcggaata
     4501 ccgattagca gattaggatc gggcgagagg caagtccaaa ccgcgcgctg cgaagcataa
     4561 gcgacacccg gaaaattcgc atctaggtca agcagatcaa gaatgatttg gaaatggaga
     4621 tgaggggccc agccgccgtt ttcttcggat gtgccaatgc gcgcaaatgt ttctccgcga
     4681 gcgacgcgct gcccaatttt cagtcccacg agtgattctg tgtcgaggtg cccatacaaa
     4741 gtaaagaatt cgccggcgtc accggcgtca t
//
LOCUS       DATCBB010000295         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_431130, whole genome shotgun sequence.
ACCESSION   DATCBB010000295 DATCBB010000000
VERSION     DATCBB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431130"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VII37_03315"
     CDS             <1..324
                     /locus_tag="VII37_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068782.1"
                     /translation="GQLVREGHIHNYMRMLWGKKIIEWSASPRAALSILIELNNKYGL
                     DGRNPNSYSGIFWCLGRYDRPWGPERPIFGMVRYMSSENTARKFSVRNYIQKFTVAKQ
                     TTLTF"
     gene            565..1761
                     /locus_tag="VII37_03320"
     CDS             565..1761
                     /locus_tag="VII37_03320"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068783.1"
                     /translation="MSIFKALKKNPGSSGGDLLRRRRRKTLLANIVIISVLSFVAALV
                     ITWQSGGVGFSLPNSAGRSTVVTSPPPSVKESSAPALPSLSEQSSDGYKLETGPFAVN
                     EVAGLVLHDEKRNKDVRARIFYPVIAGKYPVIVFSHGAGGSDTCCESLTRHWATYGYI
                     TIQPIHDDSVAQRRSSGEENASFPLAVREALKNPALWESRPRDISFILDSLAGLQKRV
                     AGLNGKIDTAHIGVAGHSMGAYTAEAVAGALVDLPEHPGQNFSDPRAKAILCLSPQGP
                     GQFGLTTHSFANVSLPFMGVTGSLDNLGPLASAAWHKTPFERSQPGDKYEVFIRGANH
                     MSFITAETANSARSSEAAAILGYTNSASLAFWDAYLKDDPAAKKFLQSDALERSSHGA
                     AKLSRR"
     gene            1804..2280
                     /locus_tag="VII37_03325"
     CDS             1804..2280
                     /locus_tag="VII37_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5068784.1"
                     /translation="MSELFVYRSQMPASADVVYAFYAKPDALTRLTPPWENARVIERT
                     GSIEQPGSRVKIRLRAGPLFQTWTAEHTACEPGRMFRDVMVHGPFRRWEHTHSFIPDG
                     QNKSWLEDRVEYEFPLGWFGKFVGGGYTRRRLSRMFEWRHKVTAEAVAQEATLLSN"
     gene            complement(2273..2785)
                     /locus_tag="VII37_03330"
     CDS             complement(2273..2785)
                     /locus_tag="VII37_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5068785.1"
                     /translation="MRFEVQLVSSTETRTHTVDLERIGDQWRVILNGEPVSADVEEMA
                     PNTLSILLHGESHEIRIARSRDGQMSIQTGLREFAAEVTDQRSWRGRRLGHVEVQGRQ
                     QITAPMAGKVVRLLVKSGDTVEVGQGLLVVEAMKMQNEIRSTKSGIVERLLAEEGQTV
                     NSGETLAWIS"
     gene            complement(2782..>3226)
                     /locus_tag="VII37_03335"
     CDS             complement(2782..>3226)
                     /locus_tag="VII37_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068786.1"
                     /translation="APSGPGIRLDDGVYEGWTVPNDYDPLLSKLIAWGNSREETIARL
                     RRSLEEYTVTGIKTNAALFRRILGEPEFVRGEIHTTWLDELLLRKHHSTAVEQGSEDA
                     AAIGAAVWQAMQNAAASATPSSTAGEEAPSRWKSEARKQQLDRTP"
BASE COUNT          661 a          903 c          900 g          762 t
ORIGIN      
        1 gggcaactag tacgtgaagg tcatattcac aattacatgc gcatgttgtg gggcaagaaa
       61 attattgagt ggtcagcatc accgcgagcg gccctgtcga ttctcatcga gttgaataat
      121 aaatacggtc tcgatggccg aaaccccaat tcttatagcg gaatcttttg gtgcctgggc
      181 cggtacgacc ggccgtgggg accggagcgc cctatattcg gaatggttcg ctacatgagc
      241 tcggaaaata ccgcgcgcaa gttttcggta agaaattaca ttcaaaaatt cacggtcgcc
      301 aaacagacga cactaacctt ttaggttcga cgcgccactt gatggacgtt ccttacgccg
      361 gcctttgata tactgaactt ggcagcagga attcccgtcc accctgaccc ccagcaaaaa
      421 ttgaactgtc gtgtggtttc atttaggcct taaaggcttc ggcaatcgct aattcgaacg
      481 cagttgaaca tcttccttgc ccgaagtttg gcgcaacttt aatggcgagg ccggttttag
      541 aagtagacag gtacgcgagc aggtatgtcg atttttaaag cgctgaaaaa aaatccgggt
      601 tcaagcggtg gcgatcttct gcggaggcgc cggcgcaaaa cgctgctcgc gaatatagta
      661 attatttccg tgctcagctt cgttgcagct ttagtgatca cctggcagtc tggcggcgtg
      721 ggattttccc ttcccaattc tgcgggacga tcgacggtgg tgacgtcgcc gccgccgtcc
      781 gttaaagagt cctccgctcc cgcattgccg tccttgtccg aacaatcctc ggatgggtac
      841 aagctcgaaa ccgggccgtt cgcggtaaac gaagttgcag gcttggtgct ccatgacgag
      901 aagcgtaata aggacgtgcg tgctcggatt ttttacccgg tgattgccgg caaatatccg
      961 gtcatcgttt tttcgcatgg cgccggtgga tccgatactt gctgcgaatc gcttacgcgt
     1021 cattgggcca cttacggcta tattacgatt cagcccattc acgacgattc agtcgctcaa
     1081 cgacgcagca gcggcgagga gaacgcgagc ttcccattgg ccgtccgcga agcgcttaag
     1141 aatcccgcgc tctgggaaag ccgtccgcga gatatttctt ttattctgga ttcactcgcc
     1201 ggtctgcaga aacgtgtcgc gggtctgaat ggaaaaatcg atactgctca cataggcgta
     1261 gccggccact cgatgggcgc ttataccgcg gaagctgtcg ccggggcatt ggtcgatctt
     1321 ccggagcatc cggggcaaaa tttttctgat cctcgtgcga aggcaatctt gtgcctttcg
     1381 ccccaaggcc cgggacagtt cggcttgacc acgcactcgt tcgccaacgt gtctctgccc
     1441 tttatgggtg taactggctc gctggacaac ttgggcccgc tggcaagcgc agcctggcac
     1501 aaaactccat tcgaacgcag ccagcccggc gataaatacg aggtgttcat ccggggcgca
     1561 aatcacatgt cgttcattac cgcagaaacc gcaaattccg ctcgctcttc ggaagctgcc
     1621 gcgattctgg ggtataccaa ctccgcttcg ctggcctttt gggatgcgta cctgaaagac
     1681 gatcctgcgg caaaaaaatt cctgcagagc gatgcgctgg agcgctccag ccatggtgcc
     1741 gcaaaactat ctcgccgctg attgaatttc ctcctctgaa tccgtatcca gttatcattg
     1801 cttgtgagcg aactatttgt ttatcgcagt cagatgcccg cctccgcgga tgttgtttac
     1861 gccttttacg ccaagccgga cgcgcttacg cggctcactc ctccatggga aaatgcccgg
     1921 gtgattgaac gtacgggttc catcgaacag ccaggatcgc gagtgaagat tcggctgcgc
     1981 gccggccctt tatttcagac ctggacggcg gagcacacgg cttgcgagcc gggacgtatg
     2041 tttcgcgatg ttatggtcca tgggccgttt cgtcgctggg aacataccca ctcattcatt
     2101 cccgatggac aaaacaaaag ttggctggaa gatcgcgtgg agtatgaatt cccattaggc
     2161 tggtttggga aatttgttgg cggaggctac acgcggcgac ggttgtcgcg gatgttcgag
     2221 tggcgtcaca aagtgaccgc tgaagccgtc gcccaagaag ccacgctgct gtctaactaa
     2281 tccacgctag cgtttcgccc gagtttacgg tttggccttc ctctgccaag aggcgctcga
     2341 cgatgcctga tttcgtggag cgaatctcgt tttgcatttt catggcttcg acaaccagaa
     2401 ggccctgtcc cacttccact gtgtcaccgg atttcaccag cagccgaacc accttgccgg
     2461 ccatcggggc ggtgatctgc tggcgccctt gcacttctac gtgaccgagg cgcctcccgc
     2521 gccaggaacg ttggtcggta acttcagccg caaattcgcg caggccagtt tgaatgctca
     2581 tctgtccatc gcgggagcgg gcaatgcgaa tttcgtgcga ctcgccgtga aggagaattg
     2641 agagcgtgtt tggggccatc tcctcaacgt cagcagagac tggctcgcca ttcaggatga
     2701 cgcgccactg gtcgcctatc cgttcgaggt caacggtgtg agttctcgtt tcggtcgagg
     2761 acaccaattg cacttcgaat ctcatggcgt gcgatccagt tgctgcttgc gggcctcgct
     2821 cttccatcgc gaaggcgctt cttcgccggc ggtcgaggac ggcgttgccg atgcagccgc
     2881 attttgcatg gcctgccaaa ctgccgctcc gatggcggca gcgtcttcgc tgccttgctc
     2941 aaccgcggtg gaatgatgtt tgcgcagcag caattcatcg agccaggtgg tatgaatttc
     3001 gcctcgaaca aattcgggtt cgccaagtat tcggcggaat aaagccgcgt tggttttgat
     3061 gccggtaacg gtgtactcct cgagcgaacg ccgaagccgc gcaatcgttt cttcgcggct
     3121 attgccccag gcgatcaact tgctgagcaa cggatcgtaa tcgttcggaa cggtccagcc
     3181 ctcatagacc ccgtcgtcga ggcgaatgcc cgggccgctg ggcgcg
//
LOCUS       DATCBB010000296         1530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_268981, whole genome shotgun sequence.
ACCESSION   DATCBB010000296 DATCBB010000000
VERSION     DATCBB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268981"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /gene="murB"
                     /locus_tag="VII37_03340"
     CDS             complement(<1..845)
                     /gene="murB"
                     /locus_tag="VII37_03340"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY5068787.1"
                     /translation="MRIEDKKVLVALRDLGVELKSEASLTDITYLGIGGTTDLLRLTR
                     HEAIPEVVKLLEANGIPFKFLGGGSNLLVADNVLPWVMMQLAQPDPDVVIEGNFATVD
                     AAADLGKTVTLCAKHDLGGMEGLIGVPGTVGGALRMNAGAYGIQIGNYVREVKVYRGG
                     ARIIEILKGDQISFEYRHTSFAPDDMMLAVKLELPSKPFREILQGIRICNEKRRASQP
                     LGQKSAGCIFKNPPGASAGRMIDELGLKGHSVGDARVSDRHANFFVNAGKASAKDMLT
                     LIADVR"
     gene            complement(835..>1530)
                     /locus_tag="VII37_03345"
     CDS             complement(835..>1530)
                     /locus_tag="VII37_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY5068788.1"
                     /translation="YGTSSQADLVISDIKLEGLVSEFKLTFKGEALGSFRLPHPPGIH
                     NVRNAAAAAAVALYLSVPVELIRDGLAKFSGVGRRFDIKGVANDITVIDDYGHHPVEI
                     RATLEAAKICKFDRLLVLFQPHRYSRTQHLWNEFCSAFNLADVLVLTDIYPASEAPIE
                     GITSEALGNAIREAGHKHVFYYPSMQAGIERLLAEARPGDAILTIGAGSVSRASNEIL
                     ALLRAERPAQHAY"
BASE COUNT          309 a          440 c          445 g          336 t
ORIGIN      
        1 cgaacgtccg caatgagcgt cagcatgtct ttggcgctgg cttttccggc gttcacgaag
       61 aaatttgcat ggcggtcgct gactcgcgcg tccccgacgg aatggccctt cagtccgagc
      121 tcatcaatca tgcgtcctgc agacgctccg ggaggattct tgaaaatgca gccggcgctt
      181 ttctggccga gaggttggct tgcacggcgt ttttcgttac agatacgaat cccttggaga
      241 atttcgcgga atggcttgga gggaagttcc agcttcaccg cgagcatcat atcatcgggc
      301 gcaaaagatg tatgccggta ttcgaaggaa atttgatcgc ccttcaaaat ttcgatgatg
      361 cgcgcgccgc cgcgataaac ttttacctcc cgtacataat tcccgatctg gattccatat
      421 gccccggcat tcatccgcag ggcgccgccc accgtgccgg gaactccgat gagcccttcc
      481 atcccgccaa gatcgtgctt ggcgcacaaa gtgacggttt tgccgaggtc ggctgcagcg
      541 tctaccgtag cgaagttgcc ttcgatgacc acgtccgggt ccggttgcgc gagttgcatc
      601 ataacccacg ggagcacgtt gtcggccact agcaggtttg agccaccacc cagaaatttg
      661 aacgggatgc cgttggcttc cagcagcttt acaacttcgg gaatcgcttc gtgacgcgta
      721 aggcgaagaa ggtcggtggt gcccccaatg cccaggtagg tgatgtccgt gagcgaagcc
      781 tcgcttttca gctccacgcc aaggtcgcgc aacgccacca gtactttttt atcttcaata
      841 cgcatgttgc gcgggacgct ccgctcgcaa taacgcgaga atttcgttgc tggcgcggct
      901 gacgctgccg gccccaatag tcagaatggc gtcacccggc ctggcttccg cgagcaggcg
      961 ctcgataccg gcctgcatag acgggtaata aaaaacgtgc ttgtgcccgg cttcacggat
     1021 ggcgttgccc agcgcctcgc tggtaatgcc ctcgataggc gcttcgctgg cgggataaat
     1081 atcggtcagc accaaaacgt cggctagatt gaatgcgctg cagaattcgt tccagagatg
     1141 ttgcgtgcgc gaatagcgat gcggctgaaa gagcaccagt aagcgatcaa acttgcaaat
     1201 cttagccgct tccagcgtgg cgcgaatttc caccggatga tggccgtaat cgtcaatgac
     1261 ggtgatgtcg ttggccacgc ctttgatgtc gaaccggcgg cccacgccag agaattttgc
     1321 gaggccatcg cggatcagct cgacgggcac gctgaggtac aacgccacgg ccgctgctgc
     1381 agccgcgttg cgtacattgt ggatgcctgg ggggtgcggc agacggaagc tgccgagcgc
     1441 ttcgccttta aaagtgagct tgaactcgct gaccaggcct tccagtttta tgtcgctgat
     1501 caccagatcg gcttggctgg acgtgccgta
//
LOCUS       DATCBB010000297         1135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_463635, whole genome shotgun sequence.
ACCESSION   DATCBB010000297 DATCBB010000000
VERSION     DATCBB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463635"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..>1135)
                     /locus_tag="VII37_03350"
     CDS             complement(412..>1135)
                     /locus_tag="VII37_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY5068789.1"
                     /translation="IRQAFIASPGHSLVAADYSQIELRIMAHLSGDATLLRAFAEDRD
                     VHQATAAEVFGTPLEQVSADQRRSAKAINFGLMYGMSAFGLARQLGIARGDAQKYMDL
                     YFERYPGVKRYMEETRRQARETGYVETLFGRRLYLPEIQSRNAALRQYAERSAINAPM
                     QGTAADIIKRAMIEVDSWLQSSRTPARLIMQVHDELVLEVADEAIEIIVGQTRTHMIR
                     AADLSIPLKVDVGVGRNWDEAH"
BASE COUNT          238 a          323 c          329 g          245 t
ORIGIN      
        1 gcgatgcgca gcgcgtgctg cgcgagacca atgcggcatg ttccgacacg gccgtcactg
       61 cgcaattgtt ttcggcgcac aaagatgaag gattcgagga ggcgcgcgcg gtgatggatc
      121 gctggcttgc ttaaaaacta aaaaaaagcc ccggtggtta cttcgagagc agccaccgag
      181 gcagaccaac ccggcatggg gatgacaaac cgggtcatgc tcgctcaggg agaagagcga
      241 ggagcgtctt aaacgctcat ttggttagat ggggatggct cgcgaaagtt ccagtccttg
      301 aaagctagtt tttattaaac gctgcgtccg cagcaaaatc acgtagcgcc gaagcgtaat
      361 tctgcgccat atttattgct agcgcacgcg tttaatgggc cttttccgcg tctaatgtgc
      421 ttcatcccaa tttcggccaa cccctacatc caccttcaga gggatcgaaa gatccgcagc
      481 gcgaatcatg tgtgtccgtg tttgtcctac aattatttca attgcctcat ccgcgacctc
      541 gagcacgagt tcatcgtgca cctgcatgat cagccgcgcc ggagtgcgcg agctttgcag
      601 ccaagagtca acttcgatca tggcgcgctt gatgatgtcg gctgccgtac cctgcatcgg
      661 cgcattgatg gcgctgcgtt cagcgtattg gcgcagtgca gcgttgcgcg actgaatttc
      721 cggtaagtaa agcctgcggc cgaatagcgt ttccacatag ccggtttcgc gcgcttggcg
      781 gcgcgtctct tccatgtaac gctttacgcc gggatagcgc tcgaaataaa gatccatgta
      841 tttctgcgca tcgccgcggg ctatgcccaa ttgccgggcc agcccgaatg cggacatgcc
      901 atacatcaga ccgaagttga tggccttcgc ggagcgccgc tggtcggcgc tcacctgctc
      961 taagggagtg ccgaagactt ccgcggcagt cgcctgatgc acgtcgcgat cttccgcaaa
     1021 ggcgcgcagc agagtcgcgt cccctgatag atgcgccatg atgcgcagct cgatttgcga
     1081 atagtccgcg gccacgagcg agtggccggg cgacgcaatg aatgcctgcc tgatc
//
LOCUS       DATCBB010000298         1151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_107067, whole genome shotgun sequence.
ACCESSION   DATCBB010000298 DATCBB010000000
VERSION     DATCBB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107067"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VII37_03355"
     CDS             <1..805
                     /locus_tag="VII37_03355"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY5068790.1"
                     /translation="TRVGCGFITWSLAKRPVLLELVLAHSPAAVMLSFGSPVPFAPRI
                     KGAGAKLICQVQNMEHARAAVDVGADIIVAQGAEAGGHGLVRATFTLVPEIADYLAKA
                     APATVLVAAGGVADGRGLAAALMLGADGALIGSRFWASKEALVHAAFHKAAIDADGDA
                     TTRTTVVDIVRKADWPKPFTARVMKTRFVNEWHGREAELAESALVEREMNRYVTAMQS
                     GDPDNTGVWVGEAAGLIHDVRPAAELLQKIAEDAEHLLKDKARGHTKVG"
     gene            971..>1151
                     /locus_tag="VII37_03360"
     CDS             971..>1151
                     /locus_tag="VII37_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068791.1"
                     /translation="MIPMPYPRRIEGYAIVSEDGMLANASGIMPDQLKFEADQRFFER
                     GLDGVDVVVHGRHSHE"
BASE COUNT          239 a          311 c          349 g          252 t
ORIGIN      
        1 cacccgggtt ggctgcggtt ttattacctg gtcgctcgcc aaacgccctg tattgcttga
       61 gctcgtattg gcgcattcgc ctgctgccgt gatgctgtca ttcgggtcgc ccgtaccgtt
      121 cgccccgcgt atcaaaggcg cgggcgccaa gttgatctgc caagtgcaaa acatggagca
      181 tgcacgcgca gccgttgacg tcggcgctga cattattgtc gctcaggggg cagaagccgg
      241 cgggcatggc ttggtccgcg cgacattcac actggtaccc gaaattgccg attacctggc
      301 gaaggccgca cctgcgactg tgctggtagc ggctggtggc gttgcggacg ggcgcgggtt
      361 agctgcagcc ctgatgctcg gagccgatgg cgcgctgatc ggttcgcggt tctgggcgag
      421 caaagaggcg ctggtgcacg ccgccttcca taaggcggca atcgatgctg atggcgacgc
      481 aaccacccgc acgaccgtcg tcgacattgt ccgcaaagca gattggccta aaccatttac
      541 ggctcgggtc atgaaaaccc gatttgtgaa tgagtggcat gggcgcgaag cagagctcgc
      601 agaatccgca ttggttgaac gcgaaatgaa ccgatacgtg actgccatgc aaagtggtga
      661 tccagacaac acaggcgttt gggtcggcga agcagcgggg ctaatccacg atgtgcgtcc
      721 tgcggccgag ctgctgcaga aaatcgcgga agacgctgaa catttgctca aagataaggc
      781 gcgcggccat accaaggtgg ggtgagttta ttttcgataa tgccactggc atttcttact
      841 ggagttactc ttggtgtcgt aacaagccac tggaataata ctcaagaaaa atcactcgct
      901 tatggtatct aaggcgcaca ccgattgcga tcgggtttcc aacccaatct gatttccgct
      961 tagtcggaaa atgattccaa tgccctatcc tcgtcgcatc gaaggttatg ccattgtctc
     1021 cgaggacggc atgcttgcga atgcgtcagg tattatgccg gaccagctca agttcgaggc
     1081 cgatcagcgc ttcttcgaac gcgggctcga tggggttgat gtggtggtgc atggccgtca
     1141 ctcccatgag c
//
LOCUS       DATCBB010000299         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_235778, whole genome shotgun sequence.
ACCESSION   DATCBB010000299 DATCBB010000000
VERSION     DATCBB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235778"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VII37_03365"
     CDS             complement(<1..259)
                     /locus_tag="VII37_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY5068792.1"
                     /translation="MNESENDLNAQAKIYLGTSAFTAAGWQGTFYPAETKSKDFLSFY
                     AQHFDTVEVNSTLYGCPSPRTVENWAAKTPDNFIFSIKTPRS"
     gene            complement(386..1342)
                     /locus_tag="VII37_03370"
     CDS             complement(386..1342)
                     /locus_tag="VII37_03370"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5068793.1"
                     /translation="MRYLVTGGAGFIGSNTVDELVRRGHSVVVLDDLSGGKEENLAEV
                     RNKITFIKGSITDIEIIRRAIYEADYVIHLAARTSVPFSMKDPIETNRINIDGTLNVL
                     VASRDAKVKRVVFAASSSAYGETPTLPKTETMQPQPISPYGVTKYVGELYAQTFGRCY
                     GLENVCLRYFNIFGPRQDPTSQYSGVMAKFCSAFLGETEPVIFGDGTQSRDFTYVDNA
                     VQANLLACEAPRASGGVFNIGTGTRVNLNEVVAALGEISGKPLLARHDPPRDGDIRDS
                     QADITHARAVLGYEPTVGLEEGLQRTFAWYRATQATKTTAKS"
     gene            1551..2468
                     /locus_tag="VII37_03375"
     CDS             1551..2468
                     /locus_tag="VII37_03375"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY5068794.1"
                     /translation="MSNRITFAVVLVAALAGGAVLWQMHNQTEISRASAAVTVAAPAP
                     AVTTEERKENDAGEPREMAAPAMEAARRPVDIKKNAVVADAGSPLRAGETWQYSAIVS
                     KLSSVANLRLKVAEKRNFLGKNAWHLQAFAHTENPFRMVFALDDQFDSYSDAATLVSM
                     QYEMHLNEKGQTMDSVQRMSPAVREAAPANATAARVLPGTRDPLGMMQYLRTVDWAKT
                     PEVRSPVYDGHKLYDVHARLQGRGEPVTVPAGSFNTFKIELQVLDNGVEMKDAHFILY
                     LSNTEARLPVLLEAVMPFATARVELVKAE"
BASE COUNT          575 a          610 c          923 g          553 t
ORIGIN      
        1 tggagcgggg agttttgatg gagaaaataa aattgtcggg agttttggcg gcccagtttt
       61 ccacggttcg cggcgagggg cagccgtaga gcgtggagtt cacttcgacg gtgtcgaaat
      121 gctgcgcgta gaacgataaa aaatctttcg attttgtttc tgcgggatag aaagtgcctt
      181 gccacccagc cgcggtgaag gcagaggtgc ctaagtatat ttttgcctgg gcgtttaagt
      241 cgttctcgga ctcgttcaca acggcgagta taccgccaac gccagcgcag agagttcgac
      301 cgtccggcgt ttttgggctt tttgatcgaa cgtgaaattg cgaccgcgag ccatacacat
      361 ccagctaaaa attgctaaag aatgctcagg acttggctgt ggttttggtg gcttgggtcg
      421 cgcggtacca cgcgaaggtg cgctgcaggc cttcttcgag gccgacggtg ggttcgtagc
      481 ccaggacggc gcgggcgtgg gtgatgtcgg cctgggagtc gcgaatgtcg ccgtcgcgtg
      541 gcgggtcgtg gcgggccagg aggggcttgc cggagatttc gcccagagcg gcgacgactt
      601 cgttcaagtt gacgcgggtt ccggtgccga tgttgaagac tcctccggag gcgcgcggag
      661 cttcgcaggc gagcaggttg gcttgcacgg cgttatcgac gtaggtgaaa tcgcgggact
      721 gcgtgccgtc gccaaagatg acgggctcgg tttctcccag gaatgcgctg cagaatttgg
      781 ccataactcc ggaatactgc gaggtgggat cctggcgcgg accaaaaata ttgaagtaac
      841 ggaggcagac attttcgaga ccgtagcagc ggccgaaagt ctgggcgtaa agttcgccga
      901 catatttggt gacgccgtag ggagagatgg gctgcggctg catggtttcc gtttttggca
      961 gggtgggggt ttcgccatat gcggaagacg aagcggcgaa gaccacgcgc ttaaccttgg
     1021 cgtcacggga ggctacgaga acgtttaggg tgccatcgat attgatgcga ttggtttcga
     1081 tggggtcctt catggagaac ggcacggagg tacgcgcggc caaatggatt acgtagtcgg
     1141 cttcgtaaat ggcgcgacgg atgatctcga tgtcggtgat gctgcccttg atgaaagtga
     1201 ttttgttgcg gacttcggca aggttttcct ccttgccgcc ggaaaggtcg tcgaggacga
     1261 cgacgctatg gccgcggcgc acgagttcgt cgacggtatt tgaacctatg aagccggcgc
     1321 cgccggtcac aaggtagcgc ataagaaaga tcctccccgc acggaaactt gcggcgtcag
     1381 gctgcgatag aaaagctata agagctggcg cagacgcgct gagcgagttt tggtacgctc
     1441 aaagtatgcc gcgacgaggc gaagacgcgt aggctgccgc gagcgagata attgtacgac
     1501 ggaaatttgc agcgcaaggg acagtttcgg aggagcgtca cgcatagggt atgagcaata
     1561 ggattacatt tgcggtggtg ttggtcgcgg cgctggctgg gggagcggtg ctgtggcaaa
     1621 tgcacaacca gacggaaatt tcgcgggcgt cggcggcggt gactgtggcg gcgcctgcac
     1681 cggcggtaac gacggaagaa cgtaaagaga atgacgcggg ggagccgcga gagatggcgg
     1741 cacctgcgat ggaggccgca aggcgtccgg tggacataaa aaaaaacgca gtggtggctg
     1801 acgcaggttc gccgttacgc gcgggggaga cgtggcaata ttccgcgatt gtgtcgaaat
     1861 tgagcagcgt cgcgaatttg cgattgaagg tggcggagaa gcgcaatttt ttgggtaaaa
     1921 acgcctggca cctgcaggcg tttgcgcaca cggagaatcc gtttcgcatg gtctttgcgc
     1981 tggacgatca gtttgattcc tacagcgacg cggccacgct ggtgagcatg caatatgaaa
     2041 tgcacctgaa tgaaaagggg cagacgatgg attcggtgca gcggatgtcg ccggcggtgc
     2101 gggaggcggc gcccgcgaat gctacagcgg cgcgggtatt gcctgggacg cgcgatccgc
     2161 tggggatgat gcagtacttg cgtaccgtgg attgggcgaa gacgccggaa gtgcgcagcc
     2221 cggtttatga cgggcacaaa ttatacgatg tgcatgcgag gctgcagggg cgcggggagc
     2281 cggtcacggt gcctgcggga agtttcaata ctttcaagat cgagctgcag gttttggata
     2341 acggcgtgga aatgaaggat gcacacttta ttttgtatct gagcaatacg gaagcgcgct
     2401 taccggtatt gctggaagcg gtgatgccgt ttgctacggc gcgtgtggaa ctggtgaagg
     2461 cggagtagag gaaagtccta caaggtggtg gctggcgaat tttcatatcg cgaaccgccg
     2521 aacaaaaaca ctcgcggata ggtctctact gctgagtgcg ccttaaaatt taaggtcaag
     2581 ggcaagaccc gggttcataa ctcaactgcg aacccgggca ccctcgctga gtgcggttta
     2641 aaatttaagg tcaaaggcaa g
//
LOCUS       DATCBB010000300         1333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_431276, whole genome shotgun sequence.
ACCESSION   DATCBB010000300 DATCBB010000000
VERSION     DATCBB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431276"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1333
                     /locus_tag="VII37_03380"
     CDS             <1..>1333
                     /locus_tag="VII37_03380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY5068795.1"
                     /translation="TLILHGKAEIDKLRPARIEMVNPHMEMTSDEMDSTEVGRIVPIY
                     EAIGTFGSRAIRRAIYAALDLLDPNMPDVLPAELRARLSYPSRREALIHTHFPPPDKS
                     IDALNTFRSAAQQRLIFEEFFLYQLSLGLDRKTTRKENAIAFRLREDTIREALKRILP
                     FKPTAAQKRVLAEIAADLEKPAPMNRLLQGDVGSGKTIIALQAAVIAIENGLQAALMA
                     PTEILAVQHYLAAKRILAPAGYRVELLISGLKFRDKTAVLESIRTGEAKLVIGTHALI
                     EDKVEFARLGFVAVDEQHRFGVLQRKRLMDKSLVHGHAPHVLVLTATPIPRTLSLTLY
                     GDLDVSIIDELPPGRTPIETRMTTEPHLPGVWEFVRREVNAGHQAYIVYPVIEESKLE
                     LKAAIEEFERLSREVFPNLKLGLLHGRLSSEEKETVMQQFRKNEVQILVATT"
BASE COUNT          308 a          389 c          338 g          298 t
ORIGIN      
        1 aaacgctgat tcttcacggc aaagccgaga tagacaagct gcgtccggcg cgcattgaaa
       61 tggtgaatcc gcacatggaa atgaccagcg acgaaatgga ctctacggag gtcggtcgca
      121 tcgtccccat ttatgaagcc atcggcacgt tcggatcacg tgccattcgt cgtgccattt
      181 atgccgctct cgatttgctc gatcccaata tgccggatgt gctccccgcc gaattacgcg
      241 cgcggctttc ttacccctcg cggcgcgaag cattgattca cacccatttt ccgccgcctg
      301 acaagtcgat cgatgcgctg aatacttttc gctcggccgc gcagcagcgt ttgattttcg
      361 aagaattttt tctctaccaa ttgagtctgg gcctcgatcg caaaaccacc cgcaaggaaa
      421 atgccatagc ctttcggctt cgtgaagata ctattcgcga agcgctaaaa cgcattttgc
      481 cgtttaagcc cactgccgcg cagaaacgcg tgctggcaga aattgccgcc gacctggaaa
      541 agcccgctcc gatgaatcgc ctcctgcagg gcgacgtggg cagcggcaaa acgatcatcg
      601 ctttgcaagc ggcggtaatc gccatcgaaa acggcctgca agctgcgctc atggctccca
      661 ccgaaattct cgctgtgcag cattaccttg ccgccaaaag gattctcgct cccgcgggtt
      721 accgcgtgga attactcatc agcgggctta agtttcgcga caagactgcc gtactcgaaa
      781 gtattcgcac cggcgaggcg aaactagtca tcggcacgca tgctttgatt gaggacaagg
      841 tcgaattcgc gcgtctcggt tttgtcgctg tcgacgagca gcaccgcttt ggcgtgttgc
      901 agcgcaaacg ccttatggat aaatcgctgg tacacggaca cgcgccgcac gtcttggtgc
      961 taaccgccac tcccattcca cgcacgctct cacttacgct ctacggcgat ctcgatgttt
     1021 cgatcattga cgagttgcct cccggccgca cacccatcga aacgcgcatg actacggagc
     1081 cgcatctgcc tggagtatgg gaatttgtgc gccgagaagt taacgccggt catcaagcct
     1141 atatcgttta tcccgtgatc gaagaatcga aactggaatt gaaagccgcc attgaggaat
     1201 tcgaacgact gtcgcgcgaa gtatttccca atctgaaact tgggcttttg cacggccgtc
     1261 tttccagcga agaaaaagaa accgtgatgc agcagttccg taagaatgaa gtacaaattc
     1321 tagtggctac cac
//
LOCUS       DATCBB010000301         1028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_269120, whole genome shotgun sequence.
ACCESSION   DATCBB010000301 DATCBB010000000
VERSION     DATCBB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269120"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..>1028
                     /locus_tag="VII37_03385"
     CDS             327..>1028
                     /locus_tag="VII37_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY5068796.1"
                     /translation="MKVLVVGGGIGGITCMLALRKRGVDAQLFEQAPAFGQVGAGIQV
                     SSNAARILLKLGLGAALKKIATYPEARDYRGWDTGERLYYTPLGQKAEAHFGSPYYAA
                     HRAELLDVLLSELDEYGVRLGSRVERVDQDGRGVTLTLANGATAQGDILVGADGIHST
                     IRAQLFGKELPRYTGNVAWRGLVPAERVAHLNLGSVVGVWMGPNRSIVQYYISAGRTF
                     NWIGISRSPQPARESW"
BASE COUNT          211 a          269 c          305 g          243 t
ORIGIN      
        1 cagctctcaa agcggttatt gctgccggat gttggtgttg attccgataa agagccttat
       61 aggaagtaat ttccatggcg agttcgctta agcacttcgc cgttatactc aaatctgaat
      121 gaccgctatt ggggagtaac agcggacaaa tgtgcctttt agtgcgcaat gatttgtccg
      181 ctaatgaccc attctaggcg ctgcaggaag caatcgagcg gcgcccgacg ctggatcggg
      241 tgacaccaat gcaatagtct cattgctgca ccagctaaaa atcgatcaag ctacgagcag
      301 ataggcgtgg gtcgtttatg gccagtatga aagttcttgt cgttggcggc ggcatcggtg
      361 gcattacctg tatgctcgcc ttgcgcaaac gcggcgtcga tgcgcagctg ttcgagcagg
      421 ctcccgcgtt tggtcaagtg ggcgcaggaa ttcaagtttc gtcgaacgcc gcgcgcatcc
      481 tgctgaagct cggtcttggc gctgcgttga agaaaattgc aacctatccc gaggcccgcg
      541 attatcgtgg ctgggatacc ggcgaacgcc tttattacac gccgctgggt cagaaagcag
      601 aagcacattt cggttcgccc tactatgcgg cgcaccgtgc agagctactc gatgttctgt
      661 tgagtgagtt ggacgagtac ggggtcaggc ttggttcgcg cgtcgagcgc gtcgatcagg
      721 acggcagagg tgtaaccctg acgctagcca atggcgcgac tgcgcaaggt gatattttgg
      781 tcggcgccga tggtatccat tcaaccatcc gggcacagct ttttggcaaa gaattgcctc
      841 gctacaccgg caatgtcgcg tggcgcggtc tggtccccgc cgagcgcgtt gcgcatctca
      901 atttaggaag cgtggttggt gtctggatgg ggccaaatcg tagcatcgtt cagtattaca
      961 tttccgcggg ccgcactttc aactggattg gtatcagccg ctcgccccag cccgcacgcg
     1021 aatcctgg
//
LOCUS       DATCBB010000302         1026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_333569, whole genome shotgun sequence.
ACCESSION   DATCBB010000302 DATCBB010000000
VERSION     DATCBB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333569"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..>1026
                     /locus_tag="VII37_03390"
     CDS             306..>1026
                     /locus_tag="VII37_03390"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEY5068797.1"
                     /translation="MTNGLGGFACGTVALANTRRYHAFLMASLAPPVQRTLLVAKVDL
                     SVEYLGLKTDLGANEFAGGTISPQGFVYLESFALIDGIPTWRYAVADALLELQIFMAQ
                     GANTSYLRLELIRSSAPLRVALKPYVAYRDYHNQSHGAHPFELESAADHCRIQACAAA
                     RPFRLLVTQGQFTPAPAWYWNFWHREEAQRGLDASEDLFFPGSFSAELAIHIPMFLIA
                     TAETAAPAAGSEVVKAIQNDSK"
BASE COUNT          207 a          296 c          287 g          236 t
ORIGIN      
        1 agccgccgaa ttcacgccgc atttccggca ccagaaagga cacgttcaac gtgtgcagct
       61 tgtccggcag gatgtgggtc ttgaatcgat cgggaaacac taaaatcgtg tcataggcca
      121 cggaaccgca aatcaatgca gcgggttcct tcggaatttt tagcgccatt aggcacctag
      181 gttggatcaa accgctattc taccgtcagt ctcaaaacag tgtttgttat gaaaaggtca
      241 cgtttctgac actcggacgc caaattttag ggtcttggga tggggcttcc agctacgaat
      301 ggctggtgac gaacggcctg ggcggatttg catgcggcac cgtggctctc gccaatacgc
      361 gccgctacca cgcttttctc atggcaagtc tggcgccgcc cgtgcagcgc acgttgctgg
      421 tcgccaaagt cgatctcagc gtcgagtatc tgggccttaa gaccgatctg ggggcgaacg
      481 aattcgccgg cggtaccatc agtccccaag gcttcgttta tttggaatcc tttgcgctta
      541 tcgatggcat tcccacctgg cgttatgctg tggccgatgc gctgttggaa ctgcagattt
      601 tcatggcgca gggcgccaac accagctatt tgcgcttgga attgattaga agcagcgcgc
      661 ccctgcgcgt cgcgctcaaa ccctacgtcg cgtatcgcga ctatcacaat caatctcacg
      721 gcgcgcaccc gtttgaattg gaatcggctg ctgatcattg ccgcattcaa gcttgcgccg
      781 cagcccggcc ctttcgtctg ctcgtcactc agggccagtt cacgccggct cccgcctggt
      841 attggaattt ctggcaccgg gaggaagctc aacgtgggct cgacgcgtcg gaggatctat
      901 ttttccccgg cagcttcagt gccgagcttg cgatacacat tccgatgttc ttgatcgcca
      961 ccgctgagac cgcggcgccg gctgcgggaa gcgaggtcgt gaaggcaatt caaaacgaca
     1021 gcaagt
//
LOCUS       DATCBB010000303         1447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_107177, whole genome shotgun sequence.
ACCESSION   DATCBB010000303 DATCBB010000000
VERSION     DATCBB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107177"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VII37_03395"
     CDS             <1..1044
                     /locus_tag="VII37_03395"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY5068798.1"
                     /translation="PEEIRWIPAQTESRVDRIVVRPGAIVKPDTIILELSNPSLQRDA
                     LDAEYQLKGAEADYENLKVQVNSELLNQKAQAAAVRSEYEQAHLQHDVDDKLHKEGLG
                     ADVTAKLSKVKDEQLGIRAQLEDQRTMIAGDSAVARLAAQRAHVDQQRALHQLRRSEL
                     EALHVRAGIDGVLQLLPVDVGQHVTPGTNLARVADPKKLKAEIKIAETQAKDATIGQK
                     ATIDTRNGIVSGHVSRIDPSVQNGTVTVDVAIDQALPQGARPDLSVDGTIEIENLKDV
                     LYVGRPVHGQSDSTVGIFKLEEDGTDATRVNVKLGRSSVNTIEIVSGLKVGDKVILSD
                     MSAWDNFDRIRLK"
     gene            1070..>1447
                     /locus_tag="VII37_03400"
     CDS             1070..>1447
                     /locus_tag="VII37_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068799.1"
                     /translation="MRTLWQDVRYGLRMMAKNPGFASIAILTLALGIGANTAIFSLID
                     QIMLRRLPVKSPDELVILRSPGRGEGHVWTDGDGAQSFSYPAYKGLRDSTAVAGMLAR
                     FAFEASIASHGQTERGLGELVSGN"
BASE COUNT          348 a          369 c          446 g          284 t
ORIGIN      
        1 cccgaagaga ttcgctggat accggcacaa acggaatcgc gtgtggaccg catcgtggtc
       61 cgccccggcg ccatcgtgaa acccgacact ataattcttg aactgagcaa tccctccctg
      121 caacgcgacg ctctcgatgc ggaatatcag ctcaagggag cggaggcgga ttacgagaat
      181 ttgaaggtgc aggtaaatag cgaactgcta aatcagaaag cccaggcggc tgctgttcga
      241 agcgaatatg aacaagccca tttgcaacat gacgttgacg acaagctgca caaggagggt
      301 ctcggcgcgg atgtaaccgc aaagctatcc aaggtaaaag acgagcagtt gggtattcgc
      361 gctcagctgg aagatcagcg cacgatgatc gccggtgact cagcggtggc aagacttgcg
      421 gcgcaacggg cgcacgtaga tcagcagcgg gcgttacatc aactgcggcg cagcgaactg
      481 gaagcattgc atgtgcgcgc gggcattgac ggagtattgc aactgctgcc ggtggatgtt
      541 ggccaacacg tgacaccggg aacaaacctg gcgcgggttg ccgatcccaa gaaactgaaa
      601 gcggaaatta agatcgcgga gacgcaggca aaggatgcga cgatcgggca aaaagccacg
      661 atcgacactc gtaatggcat agtttccggc cacgtttcgc gcattgatcc atcggtacag
      721 aacggcacgg tgacggtgga cgtagccata gaccaagcgc ttccccaagg cgcgcgtccg
      781 gaccttagcg tcgacggcac gatcgaaatc gaaaatctga aagacgtgct gtacgtgggg
      841 cgcccggtcc acggccagtc agacagcacg gtgggaattt tcaagttgga agaagacgga
      901 acggatgcta cacgcgtcaa tgtaaagttg gggcgcagtt ccgtgaatac tattgagatc
      961 gtttcgggct taaaagttgg agacaaagtg attctctccg acatgtctgc ttgggataac
     1021 ttcgatcgca ttcgtttgaa atagcgattg gtttgggtgg gagctgtcga tgcgcacgtt
     1081 gtggcaggac gtccggtatg gcctacgcat gatggcgaaa aatccgggct ttgcgtccat
     1141 tgcgatcctt acgctagcac taggtatcgg cgccaatacg gcaatttttt ctctcataga
     1201 ccagattatg ttgcggcgct tgccggtgaa gagtcccgac gagctggtca tcctgcgttc
     1261 accgggtcgc ggcgaggggc acgtgtggac tgacggcgac ggcgcgcagt ccttttccta
     1321 cccggcctac aagggattgc gtgacagtac cgcggtggcc ggaatgttag cgcgctttgc
     1381 tttcgaagcc agcatcgcta gccatggaca aaccgagcgc gggctcgggg aacttgtttc
     1441 cggaaat
//
LOCUS       DATCBB010000304         1145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_235877, whole genome shotgun sequence.
ACCESSION   DATCBB010000304 DATCBB010000000
VERSION     DATCBB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235877"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VII37_03405"
     CDS             complement(<1..398)
                     /locus_tag="VII37_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068800.1"
                     /translation="MKRMIHKVATSLTFIVLVAIAARMTFAWDQAHKISPDVLAIVPF
                     QQETGNIAYSLAQGQGFGGVFRINTGPTAWLAPIYPLMIAGIFKIFGVFTTRAFFAAV
                     FLNIIFSSAACVPVFFAAKRIAGVGTAAFAA"
     gene            508..>1145
                     /locus_tag="VII37_03410"
     CDS             508..>1145
                     /locus_tag="VII37_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5068801.1"
                     /translation="MTLSGPAIQLDHISRHYVMGASSVRAVDDLSLVIGQNEFLALLG
                     SSGSGKSTLLNLIGGLDRPTSGSIVSNGQNIATLSSLDLARYRRNTVGMIFQSFNLLT
                     RMTLEENVELPLRLAEVNRAERVGRVREALERVRLTHRLGHRPVELSGGEQQRVAIAR
                     ALVNRPKILLADEPTGNLDSVTGEAILTLLQEQTKYGMTIVMVTHERALAEK"
BASE COUNT          268 a          314 c          305 g          258 t
ORIGIN      
        1 gcagcgaaag cggccgtacc cactcccgcg attcgttttg cggcaaagaa aacaggtacg
       61 catgctgccg aggaaaaaat gatattcagg aagaccgcag caaagaaagc tcgcgttgtg
      121 aaaactccaa aaattttgaa tatccccgcg atcattaatg gataaatggg cgcaagccag
      181 gccgtggggc cggtgtttat tctaaacacc ccaccgaatc cctggccttg ggccagcgaa
      241 taggcgatgt tcccggtttc ctgctgaaac ggaacgattg ccaacacatc tggcgaaatc
      301 ttgtgcgctt gatcccacgc gaaggtcatt cgcgccgcga tagccacaag cacgatgaag
      361 gtcagcgatg tcgcgacctt gtggatcatt cgtttcatgc gcgaatccct ggggcagcaa
      421 ttaatccggc caaggtgaaa tttttatcct cattcccacg tatcattcat tgacgctaac
      481 attgacgcta gccttgacgc taaaatcgtg actctttccg gccccgccat ccaactcgat
      541 catatctcgc gccactacgt catgggcgcg agcagcgtgc gtgctgttga tgatctatct
      601 cttgtcatcg ggcagaatga atttctcgcg cttcttggct cctcgggttc tggcaagtcc
      661 acgttgttga atttaattgg cgggctggat cgcccgacct ccggttccat cgtgtcgaat
      721 gggcagaata tcgccacact ttcgtctcta gacctggcgc gctatcgccg caataccgtg
      781 gggatgattt ttcaatcctt caatctgctt acacgcatga cgctggaaga aaatgttgag
      841 ctgccgctac gactggcgga agtgaatcgc gcagaacgcg tcggccgggt gcgggaagcg
      901 ctggaacggg tgcgattgac tcatcggtta ggccaccgtc cggtggaact ttccggcggc
      961 gagcagcagc gcgtggccat cgcgcgtgcg ctagtgaatc gcccaaaaat tcttctggct
     1021 gatgaaccaa cagggaatct cgattccgtc accggcgaag ccattctcac attgctgcag
     1081 gaacaaacga aatacggcat gaccatcgta atggttacac acgagcgtgc gctggcagag
     1141 aaatt
//
LOCUS       DATCBB010000305         2042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_107201, whole genome shotgun sequence.
ACCESSION   DATCBB010000305 DATCBB010000000
VERSION     DATCBB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107201"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VII37_03415"
     CDS             complement(<1..946)
                     /locus_tag="VII37_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068802.1"
                     /translation="MSVNKSFRVATFIWLGAFALLARPGSANARGWQNVSQSPESAEP
                     ADHEDSSEMQSDRDQEQRDREQEKRDQELEKRDRDQERVDRLQELYDNGREALDDEKY
                     EQARERFAELAKANGPQTDAALYWEAYADQRLGQRQAALTAIADLKTRFPQSRWQKDA
                     SALEIEVRQNSGQPARPEAQGDEELKMLAIQSLMNSDPERAMPLLEKVLQGPATPKEK
                     AKAMFVLAQSGSPQGREIIGRIARGQSNPELQRKAVEYLGIFGGGEARKTMAEIYASS
                     SDESLKRSILRSYMIGGDKERLFAAAKSEKDPALRGEAI"
     gene            complement(943..1755)
                     /locus_tag="VII37_03420"
     CDS             complement(943..1755)
                     /locus_tag="VII37_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068803.1"
                     /translation="MKHLSEEQLIAYQDGDSSGRDVAAAHINSCAECQAELNRLAEML
                     AAYQALPVPDPGEDYGRRVWQQIAPRLEEKRSRWWQVFLEPRRLTAAGLVAALLLVAF
                     LAGRVSKRVTPSGGEVTTTATADAARVRERVLMLAVGEHLGRSEMVLMEIANAGPEGA
                     SMKQVNLSSQRRRAEDLLEENRLYRQTALQEGDTGIANVLDELERVLLDVAHSPGNVT
                     PAQFKSIRQRIDDDGLLFKVRVLTKEIERRENTGKRGPAESGSAKVERNNQA"
     gene            complement(1752..>2042)
                     /locus_tag="VII37_03425"
     CDS             complement(1752..>2042)
                     /locus_tag="VII37_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5068804.1"
                     /translation="PLRQVPDAAPTPERLTQSVELRRQMQSALQSLTPAERIAFVMRH
                     CDGCGIEEIAAALKSNSGAAKNTVFRAVHKLREILEPQFAPRATARAMGTES"
BASE COUNT          341 a          687 c          506 g          508 t
ORIGIN      
        1 gaatcgcttc tcctcgcaat gccgggtctt tttcgctctt cgccgccgcg aacaatcgct
       61 ctttgtcgcc gccaatcatg taacttcgca aaatcgagcg cttcaaagac tcatcgctgc
      121 tggaggcgta aatttcagcc atcgttttcc gcgcctcccc gccgccgaat attcccagat
      181 attccacggc ctttctttgc agctccgggt tgctttgccc ccgtgcgatt cggccgataa
      241 tctcccgccc ctgcggcgag ccgctctgag ccagcacaaa catagccttc gccttttcct
      301 tgggtgtcgc tggcccttgc aaaacttttt caagcagcgg catggcccgc tcgggatcgc
      361 tgttcatcaa actttgaata gccagcattt tcaattcttc gtcgccctgt gcttccggcc
      421 gcgccggctg cccgctgttt tgccgtactt cgatttccag cgcgctcgcg tctttctgcc
      481 aacggctttg tggaaaacga gttttcaaat ccgcgatcgc tgttaaggct gcctggcgct
      541 gccccaaccg ctggtcggca taggcctccc agtagagcgc tgcatccgtt tgcggtccgt
      601 tcgcctttgc cagctcagca aaccgctcgc gcgcttgttc atatttctcg tcatccaggg
      661 cttcgcgtcc gttgtcgtat agctcttgca atcgatccac gcgttcctga tcgcggtcgc
      721 gtttttctag ctcctggtca cgtttttctt gctcgcggtc acgctgttcc tggtcacgat
      781 cgctttgcat ctcgctgcta tcctcgtgat cggcaggttc tgcgctttcc ggcgattgcg
      841 aaacattttg ccagccgcgc gcattcgcac tccctggtct cgctagtagc gcgaacgcgc
      901 ccagccaaat aaatgtcgca acacgaaatg atttgttaac gctcatgcct ggttattcct
      961 ttcgactttt gcggagccgc tttctgccgg cccccgcttt cctgtgtttt cccgccgttc
     1021 aatttctttc gtgagcacac gaaccttaaa tagcagcccg tcgtcatcga tgcgctgccg
     1081 gatggattta aattgtgcag gcgtcacgtt tcccggactg tgcgccacgt ccagaagcac
     1141 ccgctccaat tcgtccagaa cattggcgat gccggtatcg ccttcctgca aggcggtctg
     1201 ccggtacaat ctgttttctt ccagcaaatc ttccgctcgc cggcgctgcg acgaaagatt
     1261 cacctgcttc atgcttgcgc cttcgggccc ggcgttcgca atttccatca acaccatttc
     1321 tgatctcccc agatgttctc ccacggccag catcagcacg cgctcacgta cccgcgccgc
     1381 gtcggccgtc gccgtagttg tcacttctcc acctgaagga gtgacgcgct tgctgacacg
     1441 ccctgcgaga aaagcgacga gcaaaagcgc ggcaaccagt ccggcggccg tcaatcttcg
     1501 cggctcaagg aacacctgcc accagcggct gcgcttttcc tcgagccgcg gcgcgatttg
     1561 ttgccaaacg cgccggccgt agtcttcgcc cggatcagga accggcagcg cctggtaagc
     1621 cgcgagcatt tccgcgaggc gattcaactc cgcctggcac tccgcacacg agttgatatg
     1681 cgccgccgca acatcgcgtc cggagctgtc accatcctgg taagcgatca attgttcttc
     1741 acttaagtgc ttcatgattc cgttcccatc gctctggcgg tcgcgcgcgg cgcgaattgc
     1801 ggctccaaaa tctcccgcag cttgtgtacc gcgcgaaaaa ctgtattctt cgctgcgccc
     1861 gaattggatt tcagcgccgc ggcaatttct tcaattccgc aaccgtcaca atgccgcatc
     1921 acaaacgcga tgcgctccgc gggcgtcaaa ctttgcagcg cactctgcat ctgtcttcta
     1981 agctcgacgc tctgcgtcaa ccgctccggt gtcggtgcag cgtcgggaac ctgccgcagc
     2041 gg
//
LOCUS       DATCBB010000306         1515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_139498, whole genome shotgun sequence.
ACCESSION   DATCBB010000306 DATCBB010000000
VERSION     DATCBB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139498"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VII37_03430"
     CDS             <1..490
                     /locus_tag="VII37_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068805.1"
                     /translation="VAIEKGGDVESKWAVYAALRTGDISVLPRVQRLLAKGDDQDPER
                     AIAFQLQSVTDLGAVPGLIAILETAPSEFTRNCILDVLGEKLKDQRVVPTLAAHLSDP
                     DYYARYDSLAGLQNITHEEACTLPPQWKEEDVGPQISHCKLWWEQSGKFKNWTEQRPL
                     SN"
     gene            complement(570..1331)
                     /locus_tag="VII37_03435"
     CDS             complement(570..1331)
                     /locus_tag="VII37_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5068806.1"
                     /translation="MSDLSRRKMIVSGLAATAGVAGLGVAARMAQKYGLVPPDHGGIY
                     GPGETLTYASQRVLTRHSLAREFPQSKISRPPLANEMPPLNDAYKRLQAGGFADWRVA
                     VDGMVERPASFSLEQLKSYPSRRQITMLQCEEGWSYIAEWIGVPLGQVLDAAGVRPEA
                     RYVVYFSIDPDWWDSIDMADALHPQTFLAYGMNGEELPVGNGGPVRMRLPRQLGYKSV
                     KFITRLTVTDSLKGFGKGSASASPDGGSYAWYAGI"
     gene            complement(1328..>1515)
                     /locus_tag="VII37_03440"
     CDS             complement(1328..>1515)
                     /locus_tag="VII37_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068807.1"
                     /translation="AVPSAVNLLGGQQSARTIHFFVSVFLVLFLVVHVVMIFVAGFWS
                     RMRAMITGRAETEMERS"
BASE COUNT          357 a          502 c          374 g          282 t
ORIGIN      
        1 cgttgcgatc gaaaaaggcg gcgacgttga atcaaagtgg gccgtttacg cagcgctccg
       61 caccggcgat atttccgtgc tgcccagagt gcagcgattg cttgccaagg gcgacgacca
      121 agatcccgaa cgggccatcg cgttccaatt gcaatccgtg accgatctag gcgcagtccc
      181 cggtctgatc gccatcctcg aaactgctcc aagtgagttc acccgcaact gcatactgga
      241 tgtattaggg gagaagttga aagaccaacg cgtagtgcca accctggcag ctcatctgtc
      301 cgatccagac tactatgcgc ggtatgattc gttggcgggt ctccagaaca tcactcatga
      361 agaggcttgt acattgcctc ctcaatggaa agaagaagat gtcggaccgc aaatatccca
      421 ctgcaagctt tggtgggaac aatccggcaa gttcaaaaat tggactgaac agcggcccct
      481 cagcaactag cagcgtcacg cggtaatcat gcctgcatcg acgctagaac gaatatcaaa
      541 tgtaggtcga acaagctgac ttgatcgtct taaattcccg cgtaccacgc gtagcttccg
      601 ccatctggag acgcggaggc cgaacccttg ccgaaacctt tcagactgtc ggtgactgtc
      661 agacgcgtaa taaatttcac gctcttgtat cccaactggc gcggtagccg catgcgcacc
      721 ggcccgccgt tgcccaccgg aagttcctcg ccattcatgc cgtacgcaag aaaagtttgc
      781 ggatgcaacg catcggccat atcgatgctg tcccaccagt ccggatcgat cgaaaagtac
      841 accacgtatc tggcttctgg ccgcacgccc gcggcatcca gaacctgccc gagcggcacc
      901 ccgatccact ccgctatata cgaccaaccc tcttcacact gcagcatagt aatctgcctg
      961 cgcgacgggt agctcttcag ttgctccagc gaaaacgacg cgggtcgctc gaccatgcca
     1021 tcgaccgcca cccgccagtc cgcgaatcct ccggcctgca gccgcttgta cgcatcgttc
     1081 agcggcggca tttcgttcgc cagtggcgga cgcgaaatct tcgactgcgg aaactcgcgc
     1141 gccagcgaat gtctcgtcaa cactcgttgc gaagcatacg tcaacgtctc acccggccca
     1201 taaatcccgc catgatccgg cggaacgagt ccatacttct gcgccatgcg cgcagccaca
     1261 cctaggcccg ccacgccggc cgtagccgcc agcccactca caatcatttt gcgccgcgaa
     1321 agatcgctca tgagcgctcc atctctgtct cggcccgccc ggtgatcatc gcgcgcatac
     1381 ggctccagaa tcccgcgaca aaaatcatca ccacatgcac caccaagaac agcaccagaa
     1441 acaccgaaac aaaaaagtga atcgtgcgcg ccgattgctg cccgcccaat aaattcaccg
     1501 ccgacggcac cgccg
//
LOCUS       DATCBB010000307         1173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_399081, whole genome shotgun sequence.
ACCESSION   DATCBB010000307 DATCBB010000000
VERSION     DATCBB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399081"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VII37_03445"
     CDS             <1..381
                     /locus_tag="VII37_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068808.1"
                     /translation="LAHAVPTRPLRESSTDPVLAVNLKGKWTGAEGPRHLFDVIGQCF
                     AIAQEMNCNLWRAAADLVIEPDVSEYKYDDFEHSADLIRSGEIAMRAALPEVRKWLQP
                     ETATALNASKPRGIRSANMTGDYV"
     gene            complement(574..>1173)
                     /locus_tag="VII37_03450"
     CDS             complement(574..>1173)
                     /locus_tag="VII37_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5068809.1"
                     /translation="AHLLRDRKAIVLNLGEVDFVDSSGLGAMVRALTSSRQLHGDVKL
                     CNVPETIHKVLKMTHLVKLFDIQDSEENAVAAFYRLTGSWEKLKTTGPSVLCIDRSAD
                     VLAYLRELLRRAGYQAYTTNNLHDSLILMRVTPPGLLVVGADLSASSATQQAFQSACA
                     KLPVVELGAEFSTLDAGEAASGLLERIGARLNPPSRSPS"
BASE COUNT          256 a          331 c          339 g          247 t
ORIGIN      
        1 ttagcccatg ccgttccgac gcgccctctg cgagaatcga gcaccgatcc agtattagcg
       61 gtaaatctca agggaaaatg gactggcgcg gaaggcccac gacatttatt tgacgtgatc
      121 ggacaatgct tcgccattgc gcaggagatg aattgcaatt tgtggcgtgc ggccgcggac
      181 ctcgtgattg agccggacgt gagcgaatac aagtacgacg atttcgagca cagcgccgat
      241 ttaatccggt ccggcgagat cgccatgcgc gctgcgctgc cggaagtccg gaagtggctg
      301 caaccagaaa cagccacagc cttgaacgcg tcaaaacctc gcggaatccg aagcgcaaat
      361 atgaccgggg attacgttta agtggcaggg atcgaaatta ttttaatgac gcagtcccgt
      421 tagtcccggc ctcgtttggg cacgccaaat cgttctgcgt tctaccgctc acttctcgcg
      481 aaagctacag gttcgcccac tcagttttgg ggactatcag ccgtaaacgg cgagcagcac
      541 ggccgtctaa acaatttaat ggcgggatcg acgctaagag ggtgacctgg agggcgggtt
      601 caatcgggcc cctattctct ccagcagccc ggatgccgct tcgcctgcat ccagcgtgga
      661 gaattccgct cccagttcta ctactggcag cttggcgcag gctgattgaa aagcctgttg
      721 cgttgccgag ctcgcggaga ggtccgcgcc cactaccagc aagccaggcg gcgttacccg
      781 catcaggatc aaggagtcgt ggagattgtt cgtggtgtag gcctgatagc ccgcacgccg
      841 caacaattct cgcaagtagg cgagaacgtc cgcgctgcga tcgatacata gaacgctggg
      901 gccggtggtc ttgagctttt cccacgatcc tgtaagccgg taaaacgcgg cgaccgcgtt
      961 ctcctcggaa tcctggatgt cgaacaactt caccagatgg gtcatcttca gaactttgtg
     1021 gatggtctct ggaacgttgc acagcttgac gtctccgtgc aattgacggg agctggtcag
     1081 cgctcgcacc atggctccca gcccgctgct atcgacaaaa tccacttcgc cgaggtttag
     1141 gacgattgct ttacgatcgc gtaagaggtg cgc
//
LOCUS       DATCBB010000308         1047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_301650, whole genome shotgun sequence.
ACCESSION   DATCBB010000308 DATCBB010000000
VERSION     DATCBB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301650"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..>1047
                     /locus_tag="VII37_03455"
     CDS             109..>1047
                     /locus_tag="VII37_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068810.1"
                     /translation="MAHSHGENLKQAMDTLRAHKMRSALTVFGVVLGVSVIMLVAALI
                     TGFDETIQENIKQFGADTAFVSRWDQGPHGQTRPLEERLRKPITLEDAEALKEGCPAI
                     KNVTVFLTWWEQNHTVRTKAGEVTAIDFRGVQPNFGQVYANAATLAGRFISEGDDIHR
                     EKVVMLGENVAPVLFPEGSPVGKDVLIDGSDFLVIGVVEKPKGGFGMGDEDRRVLIPY
                     STFHKIYPGAYENNIRFQAYPNMLDQSVDQATELLRRRRNVAYNAKDSFVIRTAQQEV
                     QEFHSIVGMVALAAVVLSSVGLLIGGVGVMNIMLVSV"
BASE COUNT          235 a          306 c          283 g          223 t
ORIGIN      
        1 ctcagcgtct ctaccgcagt cggtcttttc tttggaattt atcccgccat gcgtgcggcg
       61 aaactcgatc ctattgaagc gctgcgcgcg gatggctgag gcatacacat ggcccactct
      121 cacggtgaaa atctaaaaca agccatggat actctgcgcg ctcacaagat gcgcagcgcg
      181 ctcaccgttt tcggcgtggt gctgggcgtc agtgtcatca tgctggtcgc tgccctaatc
      241 accgggttcg acgaaactat tcaggaaaac ataaagcaat ttggcgctga taccgccttc
      301 gtttcccgtt gggatcaggg accacacggc cagaccaggc cgctcgaaga gcgcctgcgc
      361 aagcccatca ctctcgaaga cgcggaagct ttgaaagaag gctgccccgc gataaaaaat
      421 gtcactgtgt ttctgacctg gtgggagcaa aaccacaccg tgcgcaccaa ggccggtgaa
      481 gtgaccgcca tcgattttcg cggcgtccag ccgaattttg gtcaggtcta tgccaatgcc
      541 gcgacactcg ccggcaggtt catcagcgaa ggggacgaca ttcaccgcga aaaagtggtc
      601 atgctcggcg agaacgtcgc acccgtttta tttccggaag gatccccggt tggtaaagac
      661 gtgctgatag atggctcaga cttcctggtg atcggtgtcg tcgaaaaacc caaaggcggc
      721 ttcggaatgg gcgatgaaga ccgccgcgtg ctgatcccct acagcacctt ccacaaaatt
      781 tatcccggcg cctatgaaaa caatattcgt tttcaggcct accccaacat gctggatcag
      841 tccgtcgatc aggccaccga acttctgcgc cgtcgccgaa acgtcgctta caacgccaaa
      901 gatagtttcg tgattcgcac cgcccagcag gaagtacagg aattccacag catcgtcggc
      961 atggtagccc tcgccgcggt cgtgctgagc tccgtcggcc tgctgattgg cggcgttggt
     1021 gtaatgaata tcatgctcgt cagtgtc
//
LOCUS       DATCBB010000309         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_171897, whole genome shotgun sequence.
ACCESSION   DATCBB010000309 DATCBB010000000
VERSION     DATCBB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171897"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /gene="hemW"
                     /locus_tag="VII37_03460"
     CDS             complement(<1..901)
                     /gene="hemW"
                     /locus_tag="VII37_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY5068811.1"
                     /translation="MRRCVKVFVQMTSLGIYVQVPFCQTKCTYCNFHTGVVSSSRFAP
                     YVSVVCREIQTYRDLYSTAGIELVPAIAEAANVDTVYIGGGTPSLLDPAHLQTLLNTI
                     RDSFACEPVEVTLEADPETVDPQKASAWVRAGINRVSFGVQSFSDAELAASGRMHRRA
                     DIYRAVPILREAGIGNIGFDLIAGLPLQTPESWRDSVNRLVELGPEHISVYILEVDEK
                     SRLGSELLKGGKRYSAGAVPTEDQMAEFYELAQSSFSAAGYHHYEISNWAKPGFESRH
                     NLKYWRREAYLGFGAGAHSFSGVQ"
     gene            complement(941..1348)
                     /locus_tag="VII37_03465"
     CDS             complement(941..1348)
                     /locus_tag="VII37_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY5068812.1"
                     /translation="MPTQTLKVKTSRRTQLVDVTAQVQQAVAKSRVASGVCHVYVPHT
                     TAAVMINECADPDVASDLEGAFDRLIPFEGPYRHAEGNSDSHMKSVLVGASQTIFVED
                     GKLQLGRWQGIFFCEFDGPRERHLQVKVIQEEN"
     gene            complement(1372..>2786)
                     /gene="bshC"
                     /locus_tag="VII37_03470"
     CDS             complement(1372..>2786)
                     /gene="bshC"
                     /locus_tag="VII37_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEY5068813.1"
                     /translation="RLEKGAVAIVSGQQVGLFSGPAYAFYKALSAIQLANEVTRSGIE
                     AVPVFWMATEDHDVDEVRHVSWFQDRQLKRFELAAPPEQDAGRPVGKILLGAQIDEPV
                     HEAVELLAKQGSVLLAQFLKESYNRGETYGSAFGKLFARLFSQQGLILLDPLDAALHR
                     IAAPVYRQAIEDRDALNEQLLRRGKELEDAGFSAQVKVTAKSTLLFYMGGQCCDKAQT
                     RDGVRKAIASSGHDKFQAGDKQWAAAELLKAIESVPEDFSPSALLRAVVQDYLLPTVG
                     YIGGAAEIAYFAQSEVVYKHVLGRMPVILSRPDFTLVDIKAAKLLRAYGLKVEDIWAG
                     SQEVRRRMERASVPKDLSRDFDRNHKQVIKMLDHLHGQLKKLDPTLQGAVETARKKIE
                     YQIDKLRRKTGRAQDSKATLLAVYETFLEQLLYPHKVLQSRELCLLPFLARWGPGGLT
                     ELQKLCGSKNLGRHCIVQLS"
BASE COUNT          642 a          859 c          685 g          600 t
ORIGIN      
        1 gttgtacgcc tgagaaggaa tgtgcgcccg cgccaaaccc caaatacgcc tcgcgccgcc
       61 agtacttcaa attgtgacgc gattcgaatc ccggcttggc ccaattcgaa atttcataat
      121 ggtgataacc tgcggcagaa aatgaactct gcgccagctc ataaaattca gccatttgat
      181 cttcagtggg gactgcaccg gcgctgtaac gcttgccgcc tttcaatagc tcgctcccca
      241 gacggctctt ctcatcgacc tccaggatgt acacggagat atgctcgggg cccaattcga
      301 caaggcgatt cacagagtcg cgccaactct caggcgtctg cagcggcaac ccggcaatca
      361 agtcaaagcc gatattacca atgcccgctt cgcgaagaat cggtacagcg cgataaatgt
      421 ctgcgcgccg gtgcatgcgc ccagaagcgg ccaactcggc gtcagaaaat gactgcacac
      481 cgaaactcac gcgattgatt ccagcccgaa cccacgctga ggccttttgc ggatcgacgg
      541 tttcagggtc ggcctcgagc gttacttcca ctggttcgca cgcaaacgaa tctcgaatag
      601 tattcagaag agtttgcaga tgcgcgggat caagtaggct cggcgtgcca ccgccgatat
      661 agaccgtatc aacgttcgcg gcttccgcta tcgcaggaac aagctcgatg ccagcggtcg
      721 aatataaatc acggtaagtt tgaatttcac ggcagaccac gctcacatag ggagcgaacc
      781 gcgaagagga gaccacccca gtatgaaaat tacaatacgt gcatttcgtt tggcagaaag
      841 ggacctgcac gtagatgcca aggctggtca tttgcacaaa aactttaaca cagcgccgca
      901 ttgcgcgcag gcgccagtag cattagcgac gacgacgaaa tcagttttct tcctggataa
      961 ctttcacttg caggtgtcgc tcgcgcggcc cgtcgaattc gcaaaagaaa atcccctgcc
     1021 agcgccccaa ttgcagtttg ccgtcttcca cgaaaatcgt ctggctggcg ccaaccagca
     1081 cggacttcat atgcgaatca gaatttcctt cggcatgccg gtaagggcct tcaaacggaa
     1141 tcaagcggtc aaacgcgcct tcgagatcgc tggccacatc cggatccgcg cactcattaa
     1201 tcatgacggc cgcggtcgtg tgcggcacgt acacatggca aaccccgctg gccacccgcg
     1261 acttcgcaac cgcttgctgc acctgcgcag tcacgtcaac gagttgcgta cggcgagatg
     1321 tttttacctt tagggtctgg gtcggcacga agcaccaagc ttaacgatgg gttacgacag
     1381 ctgcactata cagtgacgcc caagattctt gctgccacac agtttttgca attccgtcaa
     1441 tccgccaggg ccccaacgcg ccagaaaagg caaaagacaa agctccctcg attgcagaac
     1501 tttgtgcggg tacagcaact gctccaggaa tgtctcgtag accgcaagca aagttgcttt
     1561 ggagtcctgt gcacgcccag tcttgcggcg aagcttatcg atctggtact cgatcttttt
     1621 acgagccgtt tccaccgccc cttgcaacgt gggatcgagc ttctttagtt ggccgtgcag
     1681 atgatccagc attttgatca cctgcttatg attgcgatca aaatcacgcg ataagtcctt
     1741 cggaaccgac gcgcgctcca ttctgcggcg aacttcctgc gaccccgccc agatgtcttc
     1801 tactttaagg ccataggccc gcagcaattt ggcggccttg atatccacca gcgtgaagtc
     1861 cgggcgcgac aatatcaccg gcatgcgccc aagcacgtgc ttgtacacca cttccgattg
     1921 cgcaaaataa gcaatttccg ccgctccacc tatgtatccc accgtgggca acaaatagtc
     1981 ctgcaccaca gcacgcaaaa gcgcgctagg gctaaaatct tcgggcacgc tttcgatcgc
     2041 cttcaacaat tccgcagccg cccactgttt atcgcccgct tgaaatttgt cgtgaccgct
     2101 gctcgcaatc gcttttcgca ctccgtctcg cgtctgcgcc ttgtcgcagc actgaccgcc
     2161 catataaaac aacaacgtgc ttttcgccgt aactttcact tgcgccgaaa atcccgcgtc
     2221 ttccagttcc ttcccgcggc gcaacaattg ttcattcaac gcgtcgcggt cttcgatagc
     2281 ctggcggtaa acgggagccg cgatgcggtg caacgccgcg tctagcggat caagcagaat
     2341 cagtccctgc tgcgaaaaca atctggcaaa caatttgccg aaggcgctcc cgtatgtttc
     2401 gccccggttg tagctctcct tcaaaaactg ggccagcaac acgctgccct gtttggccag
     2461 taactcaacg gcttcgtgaa ccggttcgtc gatctgtgcg cccagcaata ttttcccgac
     2521 cgggcgtccg gcatcctgct cgggaggcgc agcaagctcg aagcgtttca gttgcctgtc
     2581 ttgaaaccag ctcacatggc gaacttcgtc gacgtcgtgg tcttccgtgg ccatccaaaa
     2641 gaccggaaca gcctcaatcc ctgagcgcgt cacttcgttg gctagctgaa tggcgctcaa
     2701 tgccttgtaa aaggcatacg ccggaccact aaaaagtcct acctgctgcc cgctgacaat
     2761 cgccaccgct cctttttcca gtcgag
//
LOCUS       DATCBB010000310         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_399122, whole genome shotgun sequence.
ACCESSION   DATCBB010000310 DATCBB010000000
VERSION     DATCBB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399122"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VII37_03475"
     CDS             <1..588
                     /locus_tag="VII37_03475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY5068814.1"
                     /translation="ELFRLLGAGAALPVFDVNLLAAFQDAHPKSTYTLRTLNPHQNAT
                     LVTMTDLIIPETDTPGAKAVRVNEFIDLILTEWAHEDERQNFLAGLDNVDKRSNEIFG
                     KNFIDATPVQQEALLSELDQRYAMDREDLARHPFVRRPHNAQLTGDFFGVVKRMTLYG
                     YYTSEIGFTQELRKQIIPGAYHGCAPITEAGASKA"
     gene            598..2286
                     /locus_tag="VII37_03480"
     CDS             598..2286
                     /locus_tag="VII37_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5068815.1"
                     /translation="MPGTKIYDAIVIGSGITGGWAAKELTEKGLNVLLLEAGRPIVPE
                     QDYVEHVPSWELKYRGWDYREERIRTQKVQRECYYACDEYSSKFFVNDLDNPYTTDAD
                     KPFSWIRGRQVGGKSIMWGRQSYRLSDLDFEANLREGIAADWPIRYKDIEPWYEYVED
                     FVGISGQAEGLPQLPDSKFLPPMEMTCAELVVKDALPKSFNDGRMMTIGRAAVLTRVH
                     RGRAACHYCGVCHRGCITRSYFSSLNSTLPAAEKTGRMTLRPYSVVHSLIFDPGTRKV
                     TGVRVIDGQTKQSLEFRSRVLFLNASALESTRILLNSSTPEFPNGLGNTSGELGRHLM
                     DHVMGGGADGIIPGHEDKDEFGNRPNGIYVPRFRNIKTKEQFLRGYGYQGGAYREGWS
                     RGINSTGFGADFKKALRKPGAWHFSFYGFGECLPNRDNFIEINKEQKDAWGIPTLKIH
                     CAFGDNEKALLRDMSIQAAEMLTAAGATEIHPFIENNAPGLTIHEMGTARMGKDPKTS
                     VLNEHNQAHDVKNLFLTDGACMVSSACVNPSLTYMALTARACDFAVSQMKKGEL"
     gene            2290..>3537
                     /locus_tag="VII37_03485"
     CDS             2290..>3537
                     /locus_tag="VII37_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5068816.1"
                     /translation="MSQVIRSPKTYDVCIVGSGAGGGMAAKILTEGGLNVVLLEAGPQ
                     VNPEKDFKMLMWPYELPHRGAGIGGHASENFGEFLAPNGAWIIDGEPYTSAPNANFQW
                     FRSRIVGGRTNHWGRIALRFAPIDFTSGTRDGLGDDWPITYQDIAPYYDKVESYIGVF
                     GTKENVSSAPDGVFLPPPKPRCTELLVKKACDKLNIICIPARLAVLTRSINGRAPCHY
                     CGQCGRGCITGSNFSSSQVLLPPAMKTGRLTLITGAMARAVLVGKDGKAEGVSYVDKN
                     TRAENRVNARAVVLAASACESARLLLNSRSSAFPDGLANSSGTVGKYLTDSVGSDGWG
                     HVPALEKMPPHNHDGVGGMHMYIPWWKFDRKNDFPRGYHVELYGGRDMPGVGMFDELC
                     HDEEGYGAALKTRARQMYGTTIGF"
BASE COUNT          809 a          938 c          978 g          812 t
ORIGIN      
        1 gaacttttca gattgctggg agccggtgcc gctctgccgg tttttgatgt gaatctcctg
       61 gccgcatttc aggacgcaca tcccaagtct acttatacgt tgcgcaccct gaaccctcat
      121 cagaacgcca ctttggtgac catgacggat ttgatcattc cggaaacgga cacacctggg
      181 gctaaggccg tgcgcgtaaa tgaatttatc gatttgattt tgacggaatg ggctcacgaa
      241 gatgagcgtc agaattttct ggcgggcctc gacaacgtag acaaacgcag caacgaaatt
      301 tttggcaaga acttcatcga tgcgacaccc gttcagcagg aggcgcttct gagtgaactc
      361 gatcagcgct acgccatgga tcgcgaagac cttgcgaggc atcctttcgt tcgccgtcct
      421 cacaatgctc agcttaccgg cgattttttt ggcgtggtaa aacgcatgac cctttacggt
      481 tattacacat ccgagatcgg cttcacgcag gagctgcgta agcaaatcat tcccggcgcc
      541 tatcacgggt gtgcgccaat taccgaagca ggtgcgagca aggcttaggg agaattgatg
      601 cctggtacaa aaatatacga cgcaattgtc attggctccg gaatcaccgg aggctgggct
      661 gcgaaagagc tcaccgaaaa aggactgaat gttttgttgc tcgaagccgg gcgccccatc
      721 gttcctgaac aggactacgt cgagcatgtt ccttcgtggg aattgaagta tcggggctgg
      781 gattatcgcg aagagcgcat cagaacacaa aaagttcagc gcgagtgtta ttacgcgtgc
      841 gatgaatact ccagcaaatt ttttgtcaac gatctggata atccctacac caccgatgcg
      901 gataagccat tcagctggat ccgtggacgt caggtgggtg gcaagtccat catgtggggc
      961 cggcaaagtt accggctcag cgatctggat tttgaagcga atctgcgcga ggggatcgcc
     1021 gccgattggc ccatccgcta caaggacatc gagccgtggt acgagtacgt agaggatttc
     1081 gtcggcataa gcgggcaagc cgaaggtctg ccgcaacttc ccgacagcaa attcctgccg
     1141 cccatggaaa tgacgtgcgc cgaacttgtg gtcaaagatg cgctgccaaa aagttttaac
     1201 gatggccgca tgatgaccat tgggcgtgcc gcggtattga cgcgcgtgca tcgcgggcgt
     1261 gccgcgtgcc actactgcgg agtttgtcac cgcggctgca ttacccgttc gtatttcagt
     1321 agcttgaatt cgactttgcc cgccgcggaa aaaaccggcc gcatgactct tcgcccttac
     1381 agtgtggtgc acagtttgat ctttgatccg ggcactcgga aagtaactgg agtgcgcgtg
     1441 atcgatgggc agaccaaaca gtctctcgaa tttcgcagcc gggttctttt ccttaacgct
     1501 tcggctcttg aatccacacg aattttgctg aattcctcga ctccggaatt tcccaacggt
     1561 ctcggcaaca ccagcgggga gctcgggcgc catctcatgg atcacgtcat gggtggcggc
     1621 gccgatggca tcattcccgg tcacgaagat aaagatgaat tcggtaatcg cccgaatgga
     1681 atttacgttc cgcgcttcag aaatataaaa acgaaggaac agtttcttcg cgggtacggt
     1741 tatcagggcg gcgcgtaccg cgaaggctgg tcacgcggca tcaattcgac aggtttcgga
     1801 gcggatttca agaaggcgct ccgcaagcct ggtgcgtggc atttctcgtt ctatggtttc
     1861 ggtgaatgcc tacccaatcg cgacaatttt attgaaatta ataaagagca gaaggacgcc
     1921 tggggcattc ccactttaaa aattcattgc gcgtttggcg acaacgagaa agctctactg
     1981 cgagatatgt ctatccaggc ggccgaaatg ctcaccgcgg caggcgccac tgaaattcat
     2041 cccttcattg aaaataacgc accgggcctg actattcacg agatgggcac cgcgcgcatg
     2101 gggaaagatc ccaagacctc ggtcttgaac gaacataatc aggcccatga tgtaaaaaat
     2161 ctttttctca ccgacggcgc ctgcatggtt tcgtcggcat gcgtcaatcc ttcgctaacg
     2221 tacatggcgc tcacagctcg ggcgtgcgat ttcgccgtct cgcaaatgaa aaaaggtgaa
     2281 ttgtaacgga tgtctcaggt aattcgttct ccaaaaacct atgacgtttg tatcgtgggt
     2341 tcgggtgcag gcggcggcat ggccgccaaa attctcaccg aaggtggctt aaacgttgtt
     2401 ttgctggaag ctgggccgca ggtaaatccc gaaaaagatt tcaagatgtt gatgtggcct
     2461 tacgaattgc ctcatcgtgg agccggcatc ggcggtcacg cgagtgaaaa tttcggcgaa
     2521 tttctcgcgc ccaacggcgc atggatcatc gacggcgagc cctacacgtc ggcgcctaac
     2581 gctaattttc agtggtttcg ttcgcggatc gtcggagggc gcactaatca ctggggccgg
     2641 attgctcttc gattcgctcc tatcgatttt acttcaggga cacgcgatgg cttgggtgac
     2701 gattggccca tcacttacca ggacattgcg ccttattacg acaaagtcga atcctatatc
     2761 ggcgtttttg gcaccaaaga aaatgtatcc agcgctccag acggagtatt cctgccgccg
     2821 ccaaagcccc gctgcaccga actgctggta aaaaaggcct gcgacaagct gaacatcatt
     2881 tgcatcccgg cgcgcctcgc ggttttgacg cgctccatca atggccgtgc tccttgccat
     2941 tattgtggcc aatgcggacg cggttgcatc actggctcga acttcagttc cagccaggtg
     3001 ttgctgcccc cggcaatgaa aactggccga ctcactctga tcactggggc catggcccgc
     3061 gccgtgctgg tcggcaaaga tggcaaggct gaaggcgtct cgtacgtcga taaaaatacg
     3121 cgcgctgaaa accgcgtcaa tgctcgcgcc gttgttctgg cggccagcgc ctgtgaatct
     3181 gcacgcctgt tactgaattc gcggtccagt gcatttccgg acggcctcgc gaattcttcc
     3241 ggcacagtgg gaaaatatct gacggattcg gtgggcagcg atggctgggg ccacgttccg
     3301 gcgctcgaaa aaatgccgcc gcacaatcac gacggcgtcg gcggcatgca catgtatatc
     3361 ccgtggtgga aattcgatcg caaaaatgat tttccgcgcg gctatcatgt tgagttgtat
     3421 ggcggccgcg acatgccggg tgtgggaatg ttcgatgagc tttgtcacga tgaagaaggt
     3481 tacggagccg cgcttaaaac tcgcgctcga caaatgtacg gcaccactat cggattt
//
LOCUS       DATCBB010000311         1193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_139628, whole genome shotgun sequence.
ACCESSION   DATCBB010000311 DATCBB010000000
VERSION     DATCBB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139628"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VII37_03490"
     CDS             <1..697
                     /locus_tag="VII37_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5068817.1"
                     /translation="QRAGGAAAFASCDVRDYAAVEAAAKNAESQIGEINTLVNNAAGN
                     FMARTEKLTPNAFNAVVGIVLNGSFNCTQVFGKRWIEKKQPGNVLNIVTTYAAANSGS
                     GFVVPSACAKAGVLALTTSLAVEWGKHNIRLNAIAPGPFPTEGAWSRLMPSTQMEEDR
                     KNFNPMKRFGRHQELANLAAFLLSDMSPYINGECVVIDGGQWLRGAGEFNDLATLPDS
                     AWEAMESARSKKS"
     gene            complement(730..>1193)
                     /locus_tag="VII37_03495"
     CDS             complement(730..>1193)
                     /locus_tag="VII37_03495"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEY5068818.1"
                     /translation="TGDPIAFRRPEVTPDAQRLELSVELGGPWSFYAEFRRAHGLTPL
                     PHPEPSEIALQVGTTLVIPLWVRNQTSSPQEISLSAELPSGWTVQSGAGKFSIGAKQT
                     ASARLEIALPASTDAAKKNELQEITIRTASGGKSIGDVKLRVELRKRALPQ"
BASE COUNT          271 a          372 c          310 g          240 t
ORIGIN      
        1 ccagcgtgcc ggtggcgccg ccgccttcgc gtcctgcgac gttcgtgatt acgcagcggt
       61 agaagccgcc gccaaaaacg ccgaatctca aatcggagaa ataaacactc tcgtcaacaa
      121 cgccgccggg aatttcatgg cccgcaccga aaaactgacg cctaacgcat tcaacgccgt
      181 cgtcggcatc gttctcaacg gcagtttcaa ttgcacacaa gtcttcggca agcgctggat
      241 agaaaaaaaa caacccggca acgtcctcaa cattgtgacc acctacgccg ctgcgaattc
      301 cggctccgga ttcgtggtgc cctccgcatg tgcgaaagcc ggcgtgcttg cactcacgac
      361 ttctctggca gtggagtggg gaaaacacaa catccgtctc aacgctatcg cccccggccc
      421 ctttcccacc gaaggcgcat ggtcacggct tatgccctcc acgcaaatgg aagaagaccg
      481 caagaatttc aatcccatga aacgcttcgg acgtcaccag gaactcgcca acctcgccgc
      541 tttcctgctg agcgacatgt cgccctacat caatggagag tgcgtggtga tcgatggcgg
      601 ccaatggctg cgtggtgcag gagagtttaa cgatctggct acgctgccgg attcagcttg
      661 ggaagccatg gaatccgcgc gctccaaaaa atcctgagcg gttttgcttc cgaatttatt
      721 cgccaacgat tactgaggca acgcgcgctt acgcaactcc acgcgcagct ttacatcgcc
      781 gatggatttt ccgcccgatg cggtgcgaat ggtaatttct tgcagttcgt tcttcttagc
      841 cgcatcggta ctcgccggca aggcaatctc caggcgggca gacgcggtct gttttgcacc
      901 aatcgagaat ttccccgcgc cgctttgtac cgtccagccg ctcggcagtt ccgcggataa
      961 agaaatttct tgcggcgaag aagtctgatt ccgcacccac agtggaatga ccagcgtagt
     1021 cccaacttgc agcgcgatct ctgaaggttc cggatgcggc aaaggtgtca atccatgcgc
     1081 gcgacgaaac tccgcgtaaa aactccaagg cccgccaagt tcaactgaca attccagccg
     1141 ttgcgcatcg ggcgtgactt caggccgcct gaacgcaata gggtcgccgg tca
//
LOCUS       DATCBB010000312         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_366149, whole genome shotgun sequence.
ACCESSION   DATCBB010000312 DATCBB010000000
VERSION     DATCBB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366149"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..578
                     /locus_tag="VII37_03500"
     CDS             159..578
                     /locus_tag="VII37_03500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5068819.1"
                     /translation="MLINKRSIHIEWGDCDPLGIVYFPRYFEFFDACTNALFERAGLP
                     KQEMLKNYAIAGIPLVESRARFLLPSSFGETVVVESSIAEWGKASFSVYHRLFKREEL
                     AVEGFEKRVWVIHAPSEKNKIKSQAIPREVIERFEQS"
     gene            750..1433
                     /locus_tag="VII37_03505"
     CDS             750..1433
                     /locus_tag="VII37_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5068820.1"
                     /translation="MRILVAEDDAPLAEFLHQRLLQEKFAVQMVASGPEAQRYASDQS
                     YDLVVLDLNRNGSVGLDALRSIRLKKPDQPVLMVAGANMVEDRVRGLNAGADDFVAKP
                     FEVAELAARIRAVVRRGNRSGRAVLQVSDLELDRISHGVQRQGPQIDLSPKEFSLLEL
                     LMRHAGQPVSRPAIVEQVWKLNCDTMTNVVDVYINYLRRKIDVGFEVALIRSIRGVGY
                     QIGANVAPV"
     gene            complement(1530..>2003)
                     /locus_tag="VII37_03510"
     CDS             complement(1530..>2003)
                     /locus_tag="VII37_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068821.1"
                     /translation="QRSLLYSADGGAHWDSKDLSFASAGNLRLHRVDDSNLYITSNMG
                     LYTSHDAGRNWNRSDVRELQFQDVAGSGNAMVVALQKHGLLASFDAGKTWKRVDDPLA
                     EGFFPVVRVRHNGALVAASATEGLLSLELEGRSASSTGGGTAMLLPPDGVQKPKN"
BASE COUNT          422 a          486 c          594 g          501 t
ORIGIN      
        1 gccgccatag cggccaaaag gcgttcgaat cgcgtcgcaa atgtaagccg tttccatgct
       61 cacctttcgt cgtcgtgcac cggcatcaat cctattactt taccgcactc ctatgtgtag
      121 actcttgtcg atgcaccacc ctaggatttt gcgcagccat gctcattaat aagcgaagta
      181 tccatattga gtggggtgat tgcgatccgc tggggattgt ttatttccca cggtattttg
      241 aattttttga tgcttgcacg aatgcgctgt ttgaacgcgc ggggctgccc aagcaggaaa
      301 tgttgaagaa ctatgctatc gcggggattc cgctggtgga atcgcgagca cggtttcttc
      361 tcccttcttc gttcggggag actgtggtgg tcgaatccag catcgctgaa tggggcaaag
      421 ctagtttttc cgtataccac cggcttttca aaagagagga acttgctgtg gagggtttcg
      481 aaaagcgcgt gtgggtgatt cacgcgccga gtgaaaaaaa taaaatcaaa agccaagcta
      541 ttccgcgaga agttatagaa aggtttgagc agtcttagaa aactccggtt cttgcaattt
      601 ttgttggtgt tcgaggcggc tgccgagtgc ctaatcgcgc ttgcaggata atcaatttga
      661 caaccacggc gccgccggtg ttgacaactt tccgacggta tagaaactgg gtgtaccccc
      721 ttccaaatct aactcctaag tgtgagccga tgcgcattct tgttgctgaa gatgacgcac
      781 cgctggcgga atttttgcac cagcggttgc tgcaggagaa gtttgccgtt cagatggtgg
      841 ccagcgggcc tgaagcgcaa cgttatgctt cagaccagtc gtacgattta gtggtgctgg
      901 atttaaatcg caatggatcg gttggcctgg atgcgcttcg cagcattcga ttaaaaaaac
      961 cagaccagcc ggtgttgatg gtggctggcg cgaatatggt ggaagaccgc gtcagaggcc
     1021 tcaacgcggg cgcggatgac ttcgtggcca agccattcga ggtcgccgag ctggctgcgc
     1081 gcattcgtgc cgtggtgcgg cgtgggaacc gttctgggcg cgcggttttg caggtgagcg
     1141 atctggaact ggatcgcatc tcgcatgggg tacaacgaca gggtccgcag atcgatctca
     1201 gtccgaagga attttctttg ctggaacttc tgatgcggca cgcgggtcaa cctgtttcgc
     1261 gtccggcgat cgtggagcaa gtttggaagc tgaactgcga caccatgact aacgtggtgg
     1321 acgtatatat caactatctt cggcgcaaaa tagacgttgg tttcgaggtg gcgctaattc
     1381 gttctattcg cggggtcggg tatcagattg gcgcaaatgt agcgcccgtg taggtaacac
     1441 tcaagctaca gtttcatgcc ttaaagtgac gcaaaaaaaa aggcggaagg gaactgccct
     1501 tccgcctttt gattttgggt taggttgaat taatttttag gcttctgaac tccgtcgggg
     1561 ggcagcagca tagctgtgcc tccgccggtg ctgctggctg agcggccttc cagttctagt
     1621 gagagcagtc cttctgttgc ggaggctgct accagggctc cgttgtggcg tacgcgcact
     1681 actgggaaga aaccctcggc gagcggatcg tccacccgct tccatgtttt gccggcgtcg
     1741 aaggaagcaa gcaagccgtg tttttgtagc gctacgacca tggcgtttcc gcttccggcc
     1801 acgtcctgga attgcagttc gcggacgtct gagcggttcc aattgcgtcc ggcatcatgc
     1861 gaagtgtaga ggcccatgtt agaggtgatg tacagattcg aatcgtctac gcggtggagt
     1921 cgcaaattgc cggccgatgc gaaggacaaa tctttactgt cccagtgcgc tccgccgtct
     1981 gcggagtaga gcaggctgcg ctg
//
LOCUS       DATCBB010000313         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_269384, whole genome shotgun sequence.
ACCESSION   DATCBB010000313 DATCBB010000000
VERSION     DATCBB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269384"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="tuf"
                     /locus_tag="VII37_03515"
     CDS             <1..1171
                     /gene="tuf"
                     /locus_tag="VII37_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY5068822.1"
                     /translation="ERNKPHVNIGTIGHIDHGKTTLTAAITKVLSKHNPKVQFRAFDT
                     IDNAPEERERGITIAVSHVEYETQNRHYAHMDCPGHADYIKNMITGAAQMDGAILVVA
                     ATDGPMPQTREHILLARQVGVPAIVVFLNKCDMVEDAELLELVELEVRELLKSYQFPG
                     DSIAIIRGSALQALNGDAKYEKAIDELMEAVDKNVPQPVRDIDKPFAMPIEDIFSISG
                     RGTVVTGRVERGKVKVGEEIEIVGFRPTQKKVVTGVEMFRKLLDEGLAGDNVGLLLRG
                     TEKEDVERGQVVCKPGSITPHTKFKAEAYVLTKEEGGRHTPFFTGYRPQFYFRTTDVT
                     GDVKLPTGVEMVMPGDNVSCEVTLITPVALEKGLRFAIREGGHTVGAGAVTEIIE"
     gene            1175..1504
                     /gene="rpsJ"
                     /locus_tag="VII37_03520"
     CDS             1175..1504
                     /gene="rpsJ"
                     /locus_tag="VII37_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY5068823.1"
                     /translation="MRQGDKIRIRLKAYDHRILDQSTKEIVDTAKRTGADVAGPIPLP
                     TTINRWTVLRSPHVDKKSREQFEMRTHKRLIDILEPTPDTVDALMKLDLPPGVDVEIK
                     AFGHTAK"
     gene            1554..>2193
                     /gene="rplC"
                     /locus_tag="VII37_03525"
     CDS             1554..>2193
                     /gene="rplC"
                     /locus_tag="VII37_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY5068824.1"
                     /translation="MAVNGIIGIKLGMTQVFQDDGTLVGCTVLQAGPCVVVQRRTAAN
                     DGYEAAQLGLVEFVKPQRVNKAMTGHFKKAGAAPMKVLREVRIEESKDETKVGDRVLV
                     ENFKAGELVDVSGISKGKGFAGGVKRWHYRGGDATHGSMFHRAPGGIGGSSFPSRVWK
                     NQHFPGHMGHERVTAKNLKVVKVDNDENLLLVKGSVPGPAGTYIFIHKVRKAL"
BASE COUNT          533 a          580 c          664 g          416 t
ORIGIN      
        1 cgaacgtaat aagccgcacg tgaacattgg gaccattggt cacattgatc acggcaaaac
       61 aactttgacg gcggccatca ccaaggtgct gtcgaagcac aatcccaagg tgcaattccg
      121 cgcgtttgac accattgata acgcgccgga agagcgcgag cggggcatca ccattgccgt
      181 ttcgcacgtg gagtatgaaa cgcagaaccg ccactacgcg cacatggatt gcccgggcca
      241 cgcggactac atcaagaaca tgatcaccgg cgcagcgcag atggatggcg cgatcctggt
      301 ggtcgccgcg acggacggcc ccatgccgca aacacgcgag cacattttgc tggctcggca
      361 agttggcgtg cctgccatcg tggtcttcct gaataagtgc gacatggtgg aagacgcaga
      421 gttgctggaa ctggtggagc tcgaagttcg cgaactcttg aagagctacc aatttcctgg
      481 cgatagcatt gccatcattc gtggttcggc gttgcaagct ttgaacggtg acgccaagta
      541 tgaaaaggct atcgatgagc tgatggaagc ggtagacaag aatgtgccgc aaccggtgcg
      601 cgacattgat aagccgtttg ccatgcctat cgaagatatt ttctccatct ccggacgcgg
      661 cacggtcgtc acgggccggg tggaacgcgg caaggtaaaa gttggcgaag aaatcgagat
      721 cgtgggcttc cgtcccacgc agaagaaagt agttaccggc gtggaaatgt tccgcaagct
      781 gctcgacgaa ggtcttgcgg gcgataacgt tggactgctc ttgcgcggca cagaaaaaga
      841 agatgtggag cgcggccagg tagtttgcaa gcccggctcg atcacgccac acaccaagtt
      901 caaagccgaa gcgtacgttt tgaccaagga agaaggcggg cggcacaccc cgtttttcac
      961 cggctaccgt ccgcagtttt atttccgcac cacggacgtc accggcgacg tgaagctgcc
     1021 gaccggcgtc gagatggtga tgcccggcga caacgtcagc tgcgaagtca cactcatcac
     1081 cccggtagct ctcgaaaagg gtttgcgctt cgcgattcgc gaaggcggcc acaccgtggg
     1141 cgcaggcgcg gtcacggaga tcatcgaata atttatgaga caaggcgaca aaatccgtat
     1201 acgcctcaag gcgtacgatc acagaattct cgaccagtcc accaaggaaa tcgtggacac
     1261 cgcaaagcgt accggagccg atgtggctgg gccgattcct ttgcccacca ccatcaatcg
     1321 ctggaccgtt ttgcgttcgc cacacgtgga caagaaatcg cgtgagcagt tcgaaatgcg
     1381 cacgcacaag cgtttgatcg atattctcga gcccacgccg gacaccgtgg acgcgctcat
     1441 gaagctcgat cttccgccgg gcgtggacgt ggaaatcaag gcttttggac ataccgcgaa
     1501 gtaacggttt tttaatttag agaatttaaa gccgaagaga agagagagaa ggcatggcag
     1561 tcaacgggat tatcggaatc aagctgggaa tgacgcaagt cttccaggat gacggcacgc
     1621 tggtcggatg cactgttttg caagccggcc cgtgtgtggt ggtgcaacgc cgcactgccg
     1681 cgaacgacgg ctacgaagcc gcgcaactgg gcctcgtgga attcgtcaag ccgcaacgcg
     1741 tcaataaagc catgaccgga catttcaaga aagccggcgc ggcgcccatg aaagtccttc
     1801 gcgaagttcg catcgaggaa tcgaaggatg aaaccaaggt aggcgaccgg gtgctggtgg
     1861 aaaacttcaa ggctggtgag ttggtggatg tcagcggcat cagcaagggc aaagggttcg
     1921 caggcggcgt gaagcgctgg cactatcgcg gtggcgacgc cactcacggc tcgatgtttc
     1981 atcgggcacc gggaggcatc ggcggcagtt cgtttccgtc gcgcgtttgg aagaatcaac
     2041 acttcccggg tcacatgggg catgagcggg tgaccgccaa gaatttgaaa gtcgtaaaag
     2101 tcgataacga cgaaaatttg ttgctggtaa agggctccgt gcctggcccg gcgggaacgt
     2161 acatttttat tcacaaagtt cgtaaagcgc tgg
//
LOCUS       DATCBB010000314         1033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_236164, whole genome shotgun sequence.
ACCESSION   DATCBB010000314 DATCBB010000000
VERSION     DATCBB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236164"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1033
                     /locus_tag="VII37_03530"
     CDS             23..>1033
                     /locus_tag="VII37_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5068825.1"
                     /translation="MARVVPATPQERIAALWSFAYFFTLLAGYYVLRPLRDQMGIMGG
                     VRNLPWMFTATFVTLLVAQPLYGELVKKLPRARFIPIVYHFFVVNLALFWLLLKLKID
                     TAMVARAFYVWVAVFSLFAVAVFWSFMADLFTADQGKRLFGFIGAGGTAGALLGPVIT
                     IGLSESLAPMNLLIVAMILLELAVFCVWRLERVATVHDGPRAEPERLGGSAFAALPEL
                     IRSPYLLGIAVWIALQSYCATMLYFEQIHMVAAHVHGAGAQTRTFAAIDLAVNLLTLA
                     TQVVVTGRLLKRFGTGVAAATLPAIYVVGFLAVFLMPTLAVVLVLQVIQRWFHFAFSN
                     PAR"
BASE COUNT          158 a          294 c          301 g          280 t
ORIGIN      
        1 cgtagcatct ttgcaacggt ggatggcacg cgtggtgcca gccacgccgc aagagcgcat
       61 cgccgcttta tggtcgtttg catatttctt cacgctgctc gcgggatatt acgtgcttcg
      121 tccattgcgc gaccagatgg gtatcatggg cggcgtgcgg aacttgccgt ggatgttcac
      181 cgcgacattc gtgacgctgc tcgttgcgca accgctctac ggtgagttgg tcaagaaact
      241 gccgcgcgcg cgcttcatcc cgatcgtcta tcattttttc gtcgttaacc tcgcgctatt
      301 ttggttgttg ctaaagctga aaattgacac tgcgatggtc gcgcgtgcgt tttacgtgtg
      361 ggtagctgta ttcagcctgt tcgctgttgc ggttttctgg tcattcatgg ccgacctatt
      421 tacggcggac cagggcaagc gattatttgg cttcatcggt gccggtggta ctgcaggcgc
      481 gctcctcggg cctgtcatca caatcgggct gtcggaatcg cttgccccga tgaatctctt
      541 gatcgttgcc atgattttac tggaactggc cgtattctgc gtctggcggc tcgaacgtgt
      601 ggccacggtc cacgatggac cgcgtgccga accggagcgc cttggtggca gcgccttcgc
      661 cgcgctaccc gagctcattc gttcacccta tctgttgggc atcgcagtct ggattgcctt
      721 gcagtcgtac tgtgcgacga tgctctattt cgaacagatc catatggttg cagcccacgt
      781 acacggtgct ggtgcgcaaa cccgcacttt tgcggcaatc gacctcgcgg tgaacctgct
      841 cactcttgca acgcaggttg tcgtcaccgg gcgattactc aagcgctttg ggacgggtgt
      901 ggctgctgcc acgctgcccg cgatttatgt ggtcggcttt ctcgccgtat ttctgatgcc
      961 gacgctggct gtggtgctcg ttcttcaggt gattcagcgc tggtttcact tcgccttcag
     1021 caatccagcg cgg
//
LOCUS       DATCBB010000315         1341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_42516, whole genome shotgun sequence.
ACCESSION   DATCBB010000315 DATCBB010000000
VERSION     DATCBB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42516"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..>1341
                     /locus_tag="VII37_03535"
     CDS             286..>1341
                     /locus_tag="VII37_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5068826.1"
                     /translation="MAGVTADPRERVSPDVAPKLSGFWALIGTQFQGAFSDNTLKWLV
                     SFLVLETAASKEERDLWFVLIVPLLFSVPFLLFSIPGGFLADKCSKRSVTIGTVVGQL
                     VVMGLATWALAQGRLGWAGVALFLMSTLGAIFGPTKYGLLPELLPEAKLSWGNGIIEF
                     LTLLAAILAALAGGYLAHVFHGRQVWSGVIFMALAVVGFLISLGISRVPAADPGRRFD
                     WNVPREFFSEVRHMSGDRPLLTAVVANTFFWFLGSLLLLNIVLYGADILHLEETRSSY
                     LLAGLSMGIGVGSLAAGFVSGKRIQMGMVLPGLAGILVLAALLSWPGIGFVSVLLLLM
                     LLGFAGGFFVVPVNALIQ"
BASE COUNT          231 a          280 c          473 g          357 t
ORIGIN      
        1 ttttgtggcc aagtggaggc aattagaagt gtcatttaca agcgcccacg ctttgcaaaa
       61 aaccggccac cctccggctc taaagtttca gtggtggggg aaaacgtgat gatcgattct
      121 tccgttaagt cgaaagatag ccacagcttc aaaaaataaa tgcaagttca aaaatgacgt
      181 caaacccaac acccgggttc ataactcaac tgcgaacccg gggcacccgg cactccgtgg
      241 gtgacccttg cggcggaatg cgctggaggc tagactgcgc cgcgcatggc tggtgtgact
      301 gcagatcctc gcgaacgagt ttcgcctgat gtggcgccga agttgagtgg gttttgggcg
      361 ttgattggta cgcaatttca gggggcgttc agcgacaaca ctttgaagtg gctggttagt
      421 tttctggtgt tggagacggc tgcgtcgaag gaagagcggg atttgtggtt tgtgctgatt
      481 gtgccgctat tgttttcggt tcccttcttg ttgttttcca ttcctggcgg atttttggcg
      541 gacaagtgca gcaaacgtag cgtgacgatt gggacggtag tggggcaatt ggtggtgatg
      601 gggctggcta cctgggcgtt ggcgcaaggg cggcttgggt gggctggcgt ggcactgttt
      661 ttgatgagca ctttgggcgc gatttttgga cctacgaaat acgggttgct gccggaattg
      721 ttgccggaag cgaagctcag ctggggaaac gggatcattg aatttttgac tttgctggcg
      781 gcaattctgg cggcgctggc cggcggatac cttgcgcatg tgtttcatgg acggcaggtg
      841 tggtccgggg tgattttcat ggcgctggcg gtggtgggat ttctgatcag cctggggatt
      901 tcgcgagtgc ctgcggcgga ccctggacgc aggtttgact ggaatgtgcc gcgagaattt
      961 ttctcggaag tgcggcacat gagtggggac cggccgctgc tgacagcggt ggtggcgaat
     1021 acgtttttct ggtttctggg gtcgctgttg ttgttgaata tcgtgcttta tggcgcggat
     1081 attctgcatc tggaggagac gcggagcagc tatttgctgg cggggttgag tatggggatc
     1141 ggggtgggca gcctggctgc ggggtttgtg tccgggaaga gaattcagat gggcatggtg
     1201 ctgcctgggc tcgcggggat tctagtgttg gctgcgctgc tttcgtggcc ggggatagga
     1261 ttcgtcagcg tgctgttgtt gctgatgctg cttggattcg ccgggggctt ttttgtggtg
     1321 ccggtcaacg cgctaatcca g
//
LOCUS       DATCBB010000316         1777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_107504, whole genome shotgun sequence.
ACCESSION   DATCBB010000316 DATCBB010000000
VERSION     DATCBB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107504"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VII37_03540"
     CDS             <1..442
                     /locus_tag="VII37_03540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY5068827.1"
                     /translation="YAELANQLQAQWDADVILFGAAGETDVSSAIASEMHRTPIDLTG
                     KTTVADLPALLSQCHVFIGNDSGAMHVAAAVGLPAVAIFGPTDPFGTAPVTPRHTIVR
                     QQPYCSPCFLRRCPIDHRCMTRITASEVEATVDPWLASVVVERV"
     gene            435..1013
                     /locus_tag="VII37_03545"
     CDS             435..1013
                     /locus_tag="VII37_03545"
                     /inference="COORDINATES: protein motif:HMM:NF020229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5068828.1"
                     /translation="MSDPRLKPAVFLDRDGTISEEVGYLNHVSRFYMFPNAPAAIRKL
                     NERGLLVFVITNQSGVGRGYYPESLVHTVHDLMIQQLVEAGAHLDGIYYCPHTSSDAC
                     ECRKPGPGMLHQASAEHSVDLTRSFIVGDRHSDVELAHRVGARSIMVRTGYGEGEILW
                     HASKWKLKPDFIAADLTAATDWILEQTLEPQI"
     gene            997..>1777
                     /locus_tag="VII37_03550"
     CDS             997..>1777
                     /locus_tag="VII37_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5068829.1"
                     /translation="MNRKSEKKTAPPSVDLRALAEYVEQFPSKTIVVLGDFVADVFEF
                     GEISRVSREAPVLILRHRETQLVPGGGANAANNLADLGAKVLPITVVGDDANGDALIH
                     YFRQKKLDTSKILRLKNWSTPTKTRFLAGWAHTVGQQVLRVDREPQNPLPDQARKKLS
                     QFLFSAIKTAGALAISDYGFGVATPALAFEASSRRKSLFPITLDARHNLHAYSRSRIT
                     TATPNEAELESQHHTSIGENLEELARAGHATFAAMKLNSLLV"
BASE COUNT          387 a          554 c          434 g          402 t
ORIGIN      
        1 ttacgcggaa ttagccaatc agcttcaagc gcaatgggac gccgacgtga tactttttgg
       61 tgccgctggt gaaacggatg tttcgtcggc catcgcttcc gaaatgcacc gcactcctat
      121 cgatctcact ggcaaaacaa cggtcgccga cctgcccgcg cttctgtcgc aatgccatgt
      181 ttttatcggc aacgattcag gtgccatgca cgttgccgcc gcagtcgggc ttcccgcggt
      241 cgcaatcttc ggccccaccg atccatttgg caccgcgccc gttacgccgc gacacaccat
      301 cgttcgtcag caaccgtatt gcagtccatg ttttctgcgg cgttgcccga tagaccatcg
      361 ctgcatgacc cgcatcaccg ccagcgaagt ggaggccacc gtagatccct ggctggcttc
      421 cgttgtggtg gagcgtgtct gatccccgcc taaaacccgc cgttttcctc gatcgcgacg
      481 gcaccatttc cgaagaagtc ggctacttga atcacgtttc tcgtttttat atgtttccca
      541 acgcgccggc agccatccgc aaactaaacg agcgcggcct tctagttttt gtgatcacca
      601 atcaatctgg agtcggccgc ggatactacc cggaatctct ggtgcacacg gtccacgatt
      661 taatgattca acagctggtt gaggcgggag cgcatcttga cggcatttat tattgccctc
      721 atacctcaag cgatgcttgc gagtgtcgca aacccggccc cggcatgttg catcaggcct
      781 ccgctgaaca cagcgtggac ttgacgcgct ctttcattgt cggagaccgc cacagcgacg
      841 tagagttggc acaccgcgtc ggcgctcgca gtatcatggt gcgcaccgga tacggcgaag
      901 gtgaaattct ttggcatgca tctaagtgga aattgaaacc cgatttcatc gccgcggatt
      961 taaccgcggc aacagattgg atcctggagc aaacgcttga accgcaaatc tgaaaaaaag
     1021 actgctcctc cgtccgtgga tttgcgtgcc ctagcagagt atgtcgagca gtttccttcg
     1081 aagaccatcg tcgtcttggg tgacttcgtc gccgacgttt tcgaattcgg tgaaatttcc
     1141 cgcgtctctc gagaagctcc cgtactcatt cttcgtcatc gcgaaacgca actcgtccca
     1201 ggcggtggag caaatgccgc caacaacctc gctgacttgg gtgccaaggt gctacccatc
     1261 acggttgtcg gtgacgatgc caacggcgac gccttgatcc actattttcg ccaaaagaaa
     1321 ttggacactt caaaaattct gcgcctcaaa aactggtcga cgcccactaa gactcgcttt
     1381 ttagctggtt gggcgcacac cgtcggtcag caggtcctgc gtgtcgatcg cgagccgcaa
     1441 aacccattac cagatcaagc ccgcaaaaaa ttaagccaat tcttattcag cgcaataaaa
     1501 accgccggcg cgctggcgat ttccgattac ggtttcggtg ttgccacccc cgcactggcc
     1561 ttcgaagctt cctccaggag aaaatcgctt tttccgatca ctctcgacgc tcgccacaat
     1621 ttgcacgcct acagccgctc gcgaatcacc accgcgaccc caaacgaagc agaactggaa
     1681 tcccagcatc acacctccat cggcgaaaac cttgaagagc tagctcgcgc gggccacgcc
     1741 acattcgccg ccatgaaact taattctttg ctcgtca
//
LOCUS       DATCBB010000317         4133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_74237, whole genome shotgun sequence.
ACCESSION   DATCBB010000317 DATCBB010000000
VERSION     DATCBB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74237"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VII37_03555"
     CDS             <1..1076
                     /locus_tag="VII37_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5068830.1"
                     /translation="NAPMPKASIKAAQGAIEWKKFPQRVPDEAYFDVPNRIRASIAQL
                     IGARADEIALTTGASTGMLSVAYGLTWKPGDEVITAQGEFPLQYTTWRPMEKREGIVL
                     KIVAPSDRFISADDLIAALTPKSKLVSVSLVRFDDASMIDAARLAAACHAQGALLLLD
                     ASQCCGATPMDVTQLGADFIVCAGYKWLLSPYGTGFFWAKSEHSANMRPGPFYWTAAE
                     GASHFGSMVMPDPKMAAGARRWDSAETASYFNLSAMAVSLELVLRMKPETVEAHNRAL
                     MELMFERLPKDRCVPASPLDPARRGPYACIAARTLEKTAALYEKLRANNVFVALREGN
                     LRVSSHLFNTERDIDKLIAVITA"
     gene            complement(1168..2859)
                     /locus_tag="VII37_03560"
     CDS             complement(1168..2859)
                     /locus_tag="VII37_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY5068831.1"
                     /translation="MNLLGPQRARVASYVSPSSSGPQFEVRWNSFHQSLGSSIRALVT
                     GPSPRKVLSGDFLADCWIEGRIPRAAVIAAALWHLVFLLAPWPELPATPRKNPAFENF
                     QLTWSGPINDFPPLKLNVAPAPTPATPSPRGDVAKPVTREGAEAFHPRQRIFTDPIAP
                     THPRQTLINTSARIVPPKILPSMPNIVLLQQTATPAKPRMEITEQMLAQLHPSERRRA
                     TVKDAAVPDVPALDGAPTEITLAPTPNAPARPKLELNAGAAPRVAQQRSSSNDAGPAP
                     ELARQNSMNSNASTLIALSATPGPVAPTAPPQGNLAARVFISPEGKQPGVLGGKPLPN
                     ENNNGGVGGTAENLGGSASGNIAGTNSTTVSISGGNPTPKNMSGLSANPQPKMDALSP
                     HKLITKIDPRARVVDAPERTGPPNFAALAPGAKPEQIFATKKMYTMLVNMPNLNSATG
                     SWILNFSELRSSSSAPHVTSADLSGPSPIKKSDPRYPPTLINEHVEGEVVLYAVIRAD
                     GSVDSVELVRGLDAQLDANAMEALSQWKFRPASRQGTPVDLEAIVHIPFRAPSEN"
     gene            complement(3050..3652)
                     /gene="rsmD"
                     /locus_tag="VII37_03565"
     CDS             complement(3050..3652)
                     /gene="rsmD"
                     /locus_tag="VII37_03565"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY5068832.1"
                     /translation="MRVIAGLYRSRILKSLKGLALRPTSNRLRETLFNVLGPGIQDSR
                     FIDMFAGTGAVGIEALSRGAEYVSFIENHAPSAALIRKNLATLKVNAGVTVICTDAIG
                     GLETLEAKVKAGDAAYDYIFIDPPYAATADYARALQKLGASRILAERGIVIVEHRKTF
                     DLSESFGTLKRVRLLRQGDAALSFYRREGTVQSFTNADNL"
     gene            complement(3680..>4133)
                     /locus_tag="VII37_03570"
     CDS             complement(3680..>4133)
                     /locus_tag="VII37_03570"
                     /inference="COORDINATES: protein motif:HMM:NF040055.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY5068833.1"
                     /translation="TTVVEVGVDVPNATVMVIEHAERFGLSQLHQLRGRIGRGAAKSH
                     CILMAPPYMTEEARARLETMVRTANGFEIAETDLLLRGPGEFFGTRQSGDMGFHIANP
                     IRDRELLELARREAFALADDPERAAELRMVLRSLPEHWQRRYHLARIG"
BASE COUNT          853 a         1080 c         1260 g          940 t
ORIGIN      
        1 gcaatgctcc tatgcctaag gcctcgataa aggctgcgca aggcgccatt gaatggaaga
       61 aatttccgca gcgtgtaccc gacgaggcct atttcgatgt acccaatcgc attcgcgctt
      121 cgattgcgca attaattggc gcacgagcag atgaaatcgc gctcaccacc ggtgcgagta
      181 ccgggatgtt gtcggtggct tatggattga cctggaaacc cggggacgaa gttatcaccg
      241 cgcagggcga gtttcctttg caatacacca cgtggcggcc gatggagaag cgcgaaggca
      301 ttgtgctgaa aatcgtagcg cccagtgatc ggtttatcag cgcggatgat ttgatcgctg
      361 ctttaacccc gaagagtaag ttggtgtcgg tcagtctggt gcgatttgac gatgcctcga
      421 tgatcgacgc agcgcggctg gcggccgcat gtcacgcgca aggcgcgtta ttgctcctgg
      481 acgctagcca gtgttgcggc gctactccca tggacgtaac acaactcggc gcggatttta
      541 tcgtatgcgc gggttacaaa tggctgctca gtccctacgg gacgggattt ttctgggcca
      601 agagtgagca cagcgccaac atgcgaccgg ggccgtttta ttggaccgct gcggaaggcg
      661 cgagtcactt cggttccatg gtaatgcctg atcctaagat ggccgcgggt gcgcgacgtt
      721 gggactcggc ggagaccgcc agctacttta atttgtcggc catggcggtg tcgttggagt
      781 tggtgcttcg aatgaaacct gaaacggtgg aggcgcataa tcgcgcgctg atggaactga
      841 tgtttgagcg cttgccgaag gatcgttgcg tgcccgcgag tccgcttgat ccggcacgcc
      901 gcggaccgta tgcatgtatt gctgcgcgaa cactggagaa aacggcggca ttatacgaga
      961 agctgcgcgc taataatgtg ttcgtggccc tgcgcgaagg caatttgcgc gtatcgtcgc
     1021 atttgttcaa caccgaacgc gatatcgata agttgatcgc ggtgattaca gcctgatttt
     1081 ttcgcggatt cttgcgcgat gacgacgcaa ggttacgtgg tcggcgcgat ccaaaaaagt
     1141 tcgtttcgca ccaaaacgaa cacgcgctta attttcgctc ggggcgcgaa acgggatgtg
     1201 tacgatggct tcgaggtcaa cgggcgtgcc ttgccgcgat gccggtctaa acttccattg
     1261 gctgagggct tccatggcat tggcatcgag ttgcgcatcc aggccgcgaa ccaattcgac
     1321 gctatctaca gagccgtcag cgcgaatcac cgcgtaaagc acgacctctc cctctacatg
     1381 ttcgttaatt agcgtgggcg gatatctggg gtcgctcttt ttgatgggcg acggacccga
     1441 gagatcagcc gacgtcacgt gcggcgcgga actcgacgag cgcagctccg agaaattcaa
     1501 gatccagctg cccgttgcac tgttgaggtt gggcatgttg accagcatgg tgtacatctt
     1561 tttcgtggca aaaatttgct cgggttttgc gccgggcgcg agcgcggcaa aattcggcgg
     1621 accggttcgt tccggtgcat cgacgacgcg cgcacgcgga tcgatttttg taatgagttt
     1681 gtgaggcgag agcgcgtcca tcttgggctg cgggttagcc gataaaccgg acatgttctt
     1741 tggcgtggga ttgccgccgc tgatgctcac agtagtcgag tttgttcccg cgatatttcc
     1801 cgaagcggag ccgccgaggt tttcagccgt tccgccaacg ccgccattat tattttcgtt
     1861 gggcaaaggt ttaccgccga gaacgccggg ttgtttgcct tcaggcgaaa tgaaaacgcg
     1921 ggccgctaga tttccttgcg gtggcgcggt aggcgcaacg ggcccaggtg ttgcggaaag
     1981 ggcgatgagc gtggaagcgt tcgagttcat cgagttctgg cgagcgagtt cgggcgcggg
     2041 tccggcatca tttgaagacg atcgctgttg cgcaacgcgt ggtgcggctc cggcattcaa
     2101 ctccaacttc gggcgggcag gtgcgttcgg agttggggct aaagtgattt cggtgggagc
     2161 gccatcaagg gccggaacat caggtactgc cgcgtctttc accgtcgccc tgcgacgttc
     2221 gcttggatgc agctgagcca gcatttgctc ggtaatttcc atgcgcggtt tcgcgggcgt
     2281 ggcagtctgc tgcaatagaa cgatattcgg catactggga agaatttttg ggggcacgat
     2341 tcgcgccgaa gtattgatca acgtttggcg agggtgtgtt ggcgcgatgg gatcggtaaa
     2401 aatacgttgg cgaggatgga acgcttcggc accttcgcgt gtgactggtt tggctacgtc
     2461 gccacgcggg cttggagttg ctggcgtcgg tgcaggagcg acgttaagtt tgagcggcgg
     2521 gaagtcattg atggggccgg accaggtaag ctgaaaattt tcaaacgcag gatttttcct
     2581 cggggtggca ggaagctccg gccagggcgc cagcaaaaaa acaagatgcc agagagctgc
     2641 agcgattacc gcggcgcgcg ggatacgccc ttcgatccag caatcggcta aaaaatctcc
     2701 gcttaagact tttcttgggg aagggcctgt aacgagcgcg cgaatgctgc tcccaagact
     2761 ttggtggaag ctgttccagc ggacctcgaa ttgcgggcct gagctcgatg gcgacacgta
     2821 agacgcgaca cgcgcgcgtt gcggtcccag aagattcaat gaattgctgg cttgcccgaa
     2881 tgacatgcag gtctcgatta tagcgtgctg tgcgctatca aatttagcaa cttcatttca
     2941 caactttatt ttgccaattc gatttgcaag gttcgatgac ctggattcac cgaaagccta
     3001 acgcagccca aagtactgac gtcttccgga gatagatgtg taagccgctc tagaggttat
     3061 ccgcattggt gaatgattgc acagttccct cgcggcgata aaagctcagc gcggcgtcgc
     3121 cctgacgaag aagacgcacg cgcttgagag tcccaaaaga ttcggacaga tcgaaagtct
     3181 tgcgatgctc gacgatgacg attcctctct cagcaagaat cctggaggcg ccgagctttt
     3241 gcaatgcgcg agcgtagtcc gcagtagccg cgtacggtgg atcgataaag atgtaatcgt
     3301 aagcggcatc gccggctttt actttggctt ccagcgtttc gagtccgccg atcgcgtccg
     3361 tgcagataac cgtcactccg gcgttcacct ttaaagtcgc gagatttttg cgaatcagcg
     3421 ccgcgctggg ggcatggttt tctatgaagg aaacatactc agcgccacga ctaagagctt
     3481 cgattcctac ggccccggtt ccggcaaaca tatctataaa gcgagaatcc tgaattcccg
     3541 ggcccagcac gttgaaaagt gtttccctta ggcggttgct tgtgggacgc agtgccaggc
     3601 ctttcaggct tttcagtatg cggctccggt aaagtccggc gattacgcgc attttattta
     3661 ggttcccact caaatattct tagccgattc gggctagatg gtagcgccgc tgccagtgct
     3721 ccggcagcga tcgcagcacc atgcgcaatt ctgcggctcg ctcgggatcg tccgcgagcg
     3781 cgaaggcttc gcggcgcgcc aattcgagca actcgcggtc gcgtatcgga ttcgcgatgt
     3841 gaaagcccat gtcgccggat tggcgcgtac caaaaaattc acccgggccg cgcaacaaca
     3901 aatcggtttc agcgatttca aatccattgg cggtacgcac catagtttcg agccgggccc
     3961 gcgcttcttc ggtcatgtat ggcggcgcca tcaaaatgca atgagacttg gcggcgcctc
     4021 gtccgatgcg tcctcgcaat tgatgcagct gagatagtcc gaagcgctcg gcatgctcga
     4081 tgaccatcac ggtggcattc ggtacatcca cgccaacttc cacgaccgtg gta
//
LOCUS       DATCBB010000318         1757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_107564, whole genome shotgun sequence.
ACCESSION   DATCBB010000318 DATCBB010000000
VERSION     DATCBB010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107564"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VII37_03575"
     CDS             complement(<1..623)
                     /locus_tag="VII37_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY5068834.1"
                     /translation="MILTTKRAGRVLALIMATVLASLAPVTAGPVSAPEFKVPVEYYK
                     LPNGLRVVLSPDHTSPIICVGVYYHIGFRIEPRDRTGFAHLFEHMMFQGSQNLGKMEF
                     VKLVQQNGGILNGSTRFDFTNYFETLPANKLETALWAEADRMGGLAVNEDNLKNQQGV
                     VGNEVKVNVLNSPYGGFPWLDMPQYANTNWYNAHNFYGDLADIEAATL"
     gene            complement(820..>1757)
                     /locus_tag="VII37_03580"
     CDS             complement(820..>1757)
                     /locus_tag="VII37_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5068835.1"
                     /translation="AETVSSPALFHETGVLWLASKKDVGFNEMTTVLTRCKVPFESLS
                     SSAIAQRYPQISSHGLDSAILETGSGVLMGRRAVAAVVQCAVSIGVEYRQAKIAPPRV
                     EDRPTSILDAISISDGAHISAGQFVFACGAWLGKIFPERLGPLIFPSRQEVFFFGVPP
                     GDSQFSARSLPTWLIQADEVYGMPDLESRGFKIAFDGHGQRVDPDTQTRVVSAESIER
                     VRAYVTKRFPALANAPIVETRVCQYENTSNGDFLIDRHPEMTNVWFAGGGSGHGFKHG
                     PAFGEYVAQQLLDGGPVETRFALASKATEQKRAVY"
BASE COUNT          380 a          534 c          452 g          391 t
ORIGIN      
        1 agcgtcgcgg cttcgatgtc cgccaggtcg ccgtaaaaat tgtgcgcgtt gtaccagttg
       61 gtgttggcgt attgcggcat gtccagccac ggaaagcctc cgtacgggct gttcaacaca
      121 ttcactttta cttcgttccc gaccacccct tgctgattct ttaaattatc ttcgttcacc
      181 gcaaggccgc ccatgcggtc ggcttcggcc cacagcgcgg tttccagctt attggcgggc
      241 agcgtctcga aataattggt gaagtcaaag cgcgtcgagc cattcaggat tccgccgttc
      301 tgttgcacca gcttgacgaa ctccatcttc cccagattct gcgagccttg aaacatcatg
      361 tgctcgaaca ggtgcgcgaa gccggtgcgg tcgcgcggct cgatgcgaaa tccaatgtgg
      421 tagtaaacgc cgacgcaaat gatcggcgag gtgtgatccg gtgacagcac cacgcgcagc
      481 ccgtttggta gtttgtagta ctccaccgga actttaaatt ctggcgcgct cactgggccc
      541 gcggttaccg gcgctaaact cgcgagcacc gtcgccataa ttagcgcgag cactcgtccc
      601 gcccgctttg tagtcaaaat catttttcct ccctgaattc tttaccgcat caccttccac
      661 attgcccgca tcattacggg cctctacggg atatttatga atgacaataa acgcaattcc
      721 atgtcagaac gagggaccgc ccctgggccg tcctttcact tcagacggat tgaaaatgca
      781 aaagtttcag cagccggatg ctggccgcag ctcagcgcgt cagtataccg cacgcttttg
      841 ttccgtcgct ttactggcca gcgcaaatcg cgtttcgacc ggaccgccat ctaacaattg
      901 ttgcgcgaca tactctccaa aggcagggcc atgtttgaag ccgtgcccgg aaccgccacc
      961 cgcaaaccat acgttcgtca tttccgggtg ccggtcgatc aaaaaatcac cgttcgaagt
     1021 attttcatac tggcatacgc gtgtttcgac gattggcgcg ttggccaggg cagggaagcg
     1081 ttttgtcacg taagcacgga cgcgctcgat agattccgct gagactacgc gcgtttgtgt
     1141 atccggatca actcgctggc cgtgaccgtc gaaagcgatc ttgaagccgc gcgattccaa
     1201 gtccggcatg ccgtacactt catccgcttg aatcagccag gtcggcaacg atcgcgcgga
     1261 aaattggctg tctccgggtg gcacgccaaa aaagaaaact tcctggcggc tcggaaatat
     1321 gagcggacca agacgttcag ggaatatttt tccaagccac gcgccgcagg caaaaacaaa
     1381 ttggcccgcc gaaatgtgcg ctccatcgct gatcgaaata gcatcgagta tggaggttgg
     1441 tctgtcctcc acacgcggcg gcgcaatttt tgcctgacga tactcgaccc ctatgctcac
     1501 cgcgcactgc actacagccg ccaccgcacg acggcccatc aacacgccgc tcccggtttc
     1561 gagaattgca gagtccaggc cgtgcgaact aatttgcgga taacgctgcg caatcgcgct
     1621 gctgctgagc gattcgaacg gcactttgca gcgcgtcaac accgtggtca tctcattaaa
     1681 tcccacgtcc ttcttgctcg cgagccacaa gacaccggtc tcgtgaaaca acgccggcga
     1741 gctcaccgtt tccgcga
//
LOCUS       DATCBB010000319         4453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_334044, whole genome shotgun sequence.
ACCESSION   DATCBB010000319 DATCBB010000000
VERSION     DATCBB010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334044"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VII37_03585"
     CDS             complement(<1..1240)
                     /locus_tag="VII37_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEY5068836.1"
                     /translation="MADGADSSNIKNAGLPIMGAGVREKDPVCGMTVDPGKAAAKQEY
                     LGKTYYFCSQRCAEKFAKEPHRFLPVPVISGVQIARSSPSTLDVDAVHRGHASEGSPT
                     RKTPATLEAGTETTQGVAARSPLYTCPMHPQILQIGPGSCPICGMALEPVEVSAEAEV
                     DPEYESMSRRLWVSAALSVPLLVIAMAGDWLRLPLSVGARNWIELALATPVVLWGGWP
                     FFERFWMSLMNRSPNMFTLIGLGTGAAYLDSLTATIFPQIFPASFRDASGSPPIYFEA
                     AAIITTLVLLGQVLELRARQKTSGAIRALLHLAPQQAHLVAANGTERDVALNEVQRGD
                     HLRVRPGERVPTDGVVLEGQSAVDESMLTGEPMPVEKSPGDAVTGGTLNTSGSLVMEA
                     QRVGSETVLAQIVKLVSEAQR"
     gene            complement(1373..2341)
                     /locus_tag="VII37_03590"
     CDS             complement(1373..2341)
                     /locus_tag="VII37_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068837.1"
                     /translation="MLLFFTSIGSLATLSQTSSSTPPQYSVGPITDYSAAQPDYRDVL
                     RFRRGERYNSLNSPLPELGDQSDSQLFAEDTGNYYRDPMPFSHSDAVVVGNITSGQAY
                     LSNDKRDVYSEFQIIIQEVLKEPSARQIGPGDSIAVERYGGAIRLPSGKVLIRGITEF
                     SMPLVGKRYLLFLRYDQNAEDFLVQGGYQLEGEHTYSLNDLGSSPAERQHGTIVHPLK
                     EHGNSEEQLLDRARVAARPSVKGGKWDRRKSGRPERFAAASGGNYFGTRLWNDALDRD
                     IRSAQRVHDLALTQSRSVVLEGDRVMRFIMAEATQAVGVGEFSQIG"
     gene            complement(3504..>4453)
                     /locus_tag="VII37_03595"
     CDS             complement(3504..>4453)
                     /locus_tag="VII37_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5068838.1"
                     /translation="LRVLQMIIRVLHRPAVRLLTNFFEEHPADLLVSVIPHFNRQLHE
                     SWNAACPGKPFATLITDLADFPPRFWIEPIPDQYVIAGTEKAAEQARTFGHDAQHIFR
                     TSGMIVRPEFYTRENVDARELRKQMGLREDLPTALVLFGGYGSGVMHLIARRLDRDNV
                     PVQLILICGRNEKLAEKFRTENWKFPIHVVGFTKEVHHLMCAADFMIGKPGPGSIAEA
                     MVRHLPVLIECNAWTLPQERYNAEWIREKEVGIVLDNFRSVAEGVRKMVEPTTLARLK
                     NNVKALKNRALFEIPEILDGLMLEPGQQKDHQSVLAEQI"
BASE COUNT          991 a         1314 c         1112 g         1036 t
ORIGIN      
        1 tgcgctgcgc ctcgctgacc agcttcacaa tttgggccag caccgtctcg ctgcccaccc
       61 gctgcgcttc cataacgagg ctaccgctgg tgttcagcgt tccgccggta accgcgtctc
      121 caggagattt ctccacaggc atgggctcgc cggttagcat gctttcgtcg acagcgcttt
      181 gaccttcaag cacaacaccg tcggtgggca cgcgctcgcc tggacgcact ctcaagtggt
      241 cgccgcgttg cacttcattt aatgccacgt ccctctctgt gccgttggct gcaactaaat
      301 gcgcctgctg cggagccaga tgcagcagcg cccgaatcgc gccgctcgtc ttttgccgcg
      361 cgcgcaattc cagcacctga ccgagcaaca ccagcgtagt gattatcgct gctgcttcaa
      421 aataaatcgg gggcgaaccg ctggcatcgc gaaacgacgc tggaaaaatc tgcgggaaga
      481 ttgtcgccgt caggctatct agataggccg cgccggtgcc caacccgatt agcgtgaaca
      541 tattcggact gcgattcatc aacgacatcc agaatcgctc aaaaaatggc cagccgcccc
      601 acaacaccac cggcgttgcc agcgctaact cgatccaatt tcgcgctcca acggacaatg
      661 gcagccgcag ccagtcgccg gccatggcaa tcaccagcag tggaaccgaa agcgccgcgc
      721 tgacccacaa cctgcgcgac atgctctcgt attcgggatc gacctccgct tctgccgaca
      781 cttccacagg ctccagcgcc atgccacaaa tcgggcaaga cccagggcca atttgcagaa
      841 tctgtggatg catggggcag gtatacagcg gggatctagc agctacgcct tgcgtagttt
      901 ccgtaccggc ttccaatgtg gcaggcgttt ttctggtggg cgagccttcg cttgcatggc
      961 cacggtgtac ggcgtcaacg tcgagcgtgc taggactact gcgggcgatc tgcacgccgg
     1021 aaattaccgg taccggcaaa aacctatgag gctccttcgc gaatttttcc gcgcaacgct
     1081 gggaacaaaa ataataagtc tttccgagat actcttgttt cgcagccgcc ttacctggat
     1141 ctacggtcat gccgcaaacg ggatccttct cgcgcacacc cgcgcccata ataggcaacc
     1201 ctgcattttt tatattggaa gaatccgctc catccgccat agctctatgt tagatgaatc
     1261 gcactgatat tgcgttaaca gcggcgatcg aatttgcact cactgctata attccaccat
     1321 gacactcgtc gagattactt ttgagctaca atcgccgttg accgccaaac aactaaccga
     1381 tctggctgaa ttcgccaaca cctacggcct gcgtcgcttc cgccatgatg aagcgcataa
     1441 cacgatcacc ttcgagtacg acgcttcgcg actgcgtcaa agccaggtcg tgcacgcgct
     1501 gggcggagcg aatatcgcgg tcaagcgcat cgttccacaa tcgggtgccg aaataattcc
     1561 cgcctgaagc agcggcgaaa cgttccggcc gcccactttt tcgtctatcc cacttccctc
     1621 cctttacgga aggcctcgct gctaccctgg cccgatctag gagttgttct tcgctgtttc
     1681 catgttcttt taatgggtgg acaattgttc catgttgtct ttcagcgggg gacgaaccga
     1741 gatcatttag gctgtacgta tgctcgcctt cgagttgata gccgccctgg acgaggaagt
     1801 cttctgcgtt ctggtcgtat ctcaggaaca gcaggtaccg ttttccgacc aacggcatgg
     1861 aaaattcagt aataccgcgt atcagcacct tccctgatgg caggcgaatt gcgccgccat
     1921 accgctccac ggcaatgctg tcaccggggc caatttggcg ggcagaaggt tccttcaaaa
     1981 cttcctgaat gatgatctga aattccgaat agacgtctcg cttatcgttt gagagatagg
     2041 cttggcctga tgtgatattg cctacgacca cagcatcgct gtgactaaat ggcatgggat
     2101 cccggtagta gttgccggta tcctcggcaa ataattggct gtcactttgg tctcctaact
     2161 cgggcagcgg agagttcaga ctgttatatc gttcgcctcg cctaaaacgc aaaacgtctc
     2221 tgtagtcagg ctgagctgca ctgtaatcgg tgatggggcc gacgctatac tgtggtggag
     2281 ttgaactaga tgtttgcgaa agggtcgcca agctacctat cgaggtgaaa aataacaaca
     2341 caacgctaca aagaaatatt ttacttcgtg acattctcac aacctgctga ttaacgcggc
     2401 aagccgaccg gatcgttgca cgatataaca ttgctttagt atttagaaat atttaaagac
     2461 acttttaata actagtatta acaacggagc gatgaatttg aagccttttc atggagcgcc
     2521 taccgtttgg cgggcagtac aggaaggtcg tgagcgcaaa ttgattatgg atgatgtaaa
     2581 acggaaaaat cgtgactgtg gagcttcgcc tcatgtgcca acgtggactg gcgagcgagt
     2641 gcgtatcgat cagccccaaa aaaagtgggg ttcccgcttg ggaccgggaa ccccgagaag
     2701 gcgcgttcca ttgccagtgt cgttttcaca ccggcgcgtg gggtcctcca tcctagcttc
     2761 cctctcagag gcaggtggga acgatttgca aaaaatggcg agcaagcccc ttccccctca
     2821 ctcaccgcct tttacatttc gtcatgtaaa acgcgcctac acccctgcta atgcaatagg
     2881 gcggccattg ccgaacgccg cctgtgtaaa gttaaggcca ttcttatgtg tgcatcgcgt
     2941 ttttgcatcg attgatgcgc ggttccaaaa ttgaggccac gacccaattc ggaacggcac
     3001 gaccccgcat cgggaatcgc cccacttggc ctcaggctgc cttgcctctt aattcagttc
     3061 taggacctta gtaatgattt ttagtgactc tcccgagcta tgggtttttc tgagccacgc
     3121 gtgcaacatt aagcatgaac acgttgtttc tctcggtcat aactcaccga tcgttctgtg
     3181 ttcgtttgcc agccgtcctc gtcctcacgt tcttgcccat cgtttccctc tatggcgatg
     3241 tgcctgctcg cctgcgaaac atttctgcac cgggatgcta ttgccactgc catgaatccc
     3301 cggctcgtcg gggctgcatt aaattgtgcg atgccaaaaa agccgtgccc gctcgttggc
     3361 tgactacgtc ctgcgtaaaa ccgcacttcc agcccccgcc cgataaatca aatgccggcc
     3421 cacacctgca tcacccggac cacgcggaac acgcaaaact ttattgaaat cgcgacatcg
     3481 gtaattgaaa ctaacctcgg taattaaatt tgttccgcta ggactgactg gtgatccttc
     3541 tgttgccccg gctccagcat aagcccatcc aggatctcgg ggatttcaaa cagcgcccgg
     3601 ttcttgagcg ccttcacgtt gttctttagg cgtgccagcg tggtgggctc gaccatcttc
     3661 ctgactcctt ccgccacgct gcgaaaatta tccagcacga tgcccacttc tttttctctt
     3721 atccattccg cgttgtagcg ttcctgcggc aacgtccagg cattgcattc gatcaacacc
     3781 ggcaaatgcc gcaccatggc ttcggcgata ctcccggggc ctggcttgcc gatcataaaa
     3841 tccgcggcgc acatcaagtg gtgcacctct ttggtaaagc ccacaacatg aatcgggaat
     3901 ttccagtttt cagtgcgaaa tttttccgcc agtttttcgt tacggccgca aatcaaaatg
     3961 agctgcacgg gaacgttgtc gcgatccagc cgccgcgcga tcaggtgcat gacccccgaa
     4021 ccgtatcctc caaaaaggac gagagcggtg ggcaagtcct cacgcaggcc catctgcttt
     4081 cgcaactcgc gcgcgtcgac attttcacgc gtgtaaaact ccggccgcac gatcattcca
     4141 gaagtcctaa aaatgtgctg cgcatcatgg ccgaacgtgc gcgcctgttc ggcggctttt
     4201 tcggtgccgg cgatcacgta ttgatccggg atcggctcga tccagaaacg cggcggaaaa
     4261 tctgccaggt ccgtaatcag cgtagcgaac ggtttgcccg gacatgcggc attccagctt
     4321 tcatgcagct ggcgattgaa gtgcggaatt accgacacca gcaaatccgc gggatgctcc
     4381 tcaaaaaagt ttgtaagcag ccgcaccgca ggccgatgca ggacacgaat tatcatttgt
     4441 aacacccgca aca
//
LOCUS       DATCBB010000320         1003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_107620, whole genome shotgun sequence.
ACCESSION   DATCBB010000320 DATCBB010000000
VERSION     DATCBB010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107620"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VII37_03600"
     CDS             complement(<1..994)
                     /locus_tag="VII37_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5068839.1"
                     /translation="MSYLQKLDLRMNEEHPPLAKIWAAIPLVIRGTHADYSHISWTFS
                     ERKFFGAYLGQWIFGAWILERWNDAASTVAWARFPMLLLMLALGWSLFVIGRRLGGNW
                     GGLLCLAVYASSPVFLAFGPLVHTDIPVTLFTLLSLWTFAEIWREPDRKKSFLFGLCL
                     AGAILSKFTAGILFFAFGIFAFTTRWWPVAGQPETKPERRSWRRLRWRAALKGIGWAS
                     AIIYLFYFIFSIRQTTDILYFVGHGPAFVPLRRVLMPAWLFLRGLFMVVITASRPTFI
                     LGHAYPHGVWFYYPVVFVLKSQIGLLGLLLLGIAVAIFQKRRVAGPSIPPQFTVH"
BASE COUNT          290 a          284 c          263 g          166 t
ORIGIN      
        1 agtgaaccgt gaattgcgga ggaatgctcg ggccggcgac gcggcgtttt tggaagatgg
       61 ctacggcgat tcccaacagc agcaatccca acaagcctat ctgggatttc aaaacaaaga
      121 ccaccggata ataaaaccag acgccatgcg ggtaggcgtg tccgagaata aatgtcggac
      181 ggctggcggt gattacaacc ataaacaagc cgcgcaggaa gagccacgcc ggcatcagca
      241 cgcgcctcag gggaacgaac gcggggccat gaccgacgaa atacagaatg tcagtcgttt
      301 gacggatgga aaaaataaaa taaaaaaggt aaataatggc cgaagcccat ccgatgccct
      361 tcagcgctgc gcgccaacgc aaccgccgcc aactgcggcg ctctggtttg gtttccggtt
      421 gaccagcgac cggccaccaa cgcgtcgtga aggcaaaaat gccgaaggcg aaaaacagaa
      481 ttccagcggt aaatttcgaa agaatcgcgc ctgccaggca caatccaaac agaaatgatt
      541 ttttgcggtc cggttcgcgc cagatttccg cgaacgtcca caacgacaac agagtgaaga
      601 gcgttactgg gatatcagtg tgcaccagag ggccaaatgc cagaaaaacc ggagaacttg
      661 catagacagc aaggcagagc agaccacccc aattcccccc aaggcggcgg cctatcacga
      721 aaagggacca gcccaatgcc agcatcagca acagcatggg gaagcgtgcc caagccacag
      781 tgctggccgc atcattccac cgctccaaaa tccacgctcc aaaaatccat tgaccgaggt
      841 atgctccgaa aaactttctt tcgctgaaag tccatgaaat gtgggagtaa tccgcatggg
      901 ttccgcggat caccagtgga atagctgccc aaatctttgc cagcggcggg tgctcctcgt
      961 tcattcgaag atccagcttt tgcagataac tcacgcccgc ccc
//
LOCUS       DATCBB010000321         1130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_399486, whole genome shotgun sequence.
ACCESSION   DATCBB010000321 DATCBB010000000
VERSION     DATCBB010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399486"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VII37_03605"
     CDS             complement(<1..1117)
                     /locus_tag="VII37_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY5068840.1"
                     /translation="MAWFGIRVNTFANSRTAFASLRGKPYPIYHTPLQAGMSIGMMLI
                     SVELLIMLFILLFIPGDYAGPCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIV
                     FKIKEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGVALITFILLGVHSPTVQV
                     QLLVWIFIMRIVMLIASAAAYFINGAIAKARFGNVNEMNYEAPLTSLVWITSVVSIGF
                     TYLVSYLIIPSLGQDTTQWWKLATIISCGTLAGAIIPELVKVFTSTESRHVREVVSSA
                     QEGGASLGILSGFVAGNFSAYYIGLAMVVLMSLGYLISEMGLATFMIAAPVFAFGLVA
                     FGFLGMGPVTIAVDSYGPVTDNAQSVYELSLIEQIPGI"
BASE COUNT          315 a          366 c          256 g          193 t
ORIGIN      
        1 gaatcccggg aatttgctcg atcagcgaca actcataaac cgattgcgcg ttgtccgtca
       61 ccggcccgta agaatccacc gcgatagtca ccggccccat acccaggaaa ccaaatgcca
      121 ccaacccaaa cgcaaacact ggcgcggcga tcataaatgt cgccaatccc atctcgctga
      181 tcaaataacc cagcgacatg agcaccacca tggccagccc gatgtaatac gctgaaaaat
      241 ttccagccac aaaacccgac agaattccca gcgacgcgcc accttcctgt gcggaactga
      301 ccacttcccg cacgtgccgc gactccgtgg aagtaaaaac cttgaccagc tcaggaataa
      361 tcgcgcccgc caaagtgccg caagaaataa tggtcgccag cttccaccac tgcgtggtgt
      421 cctgccccag cgaaggaatg atcaggtacg aaactaggta ggtgaaccca atcgaaacta
      481 cagaagtgat ccacaccagc gaagtcagcg gagcctcata gttcatctca ttgacgttgc
      541 cgaaccgagc cttggcaatc gctccgttga tgaaataagc cgccgcgctg gcgatcagca
      601 tcacaattcg catgatgaag atccacacca gcaattgcac ctgcaccgtg gggctatgca
      661 cgcccaacaa aataaaagta atcagcgcca cgcccgtcac gccatacgtt tcaaacccgt
      721 cagcgctggg ccccacggag tcgccggcat tgtcgcccgt gcaatccgcg atgacgcccg
      781 gattgcgcgc gtcgtcttcc ttaatcttga agacgatctt catcaaatcc gagccgatat
      841 cggcaatctt ggtaaaaatt ccgcccgcga tacgcagcgc agcggcaccc agcgattcac
      901 caatggcgaa tccaataaag cacggccccg catagtcgcc gggaataaac agcaaaataa
      961 acaacatgat caacaattcc acgctgatca gcatcatccc gatgctcatg cccgcctgca
     1021 gcggagtgtg ataaatgggg taaggcttgc cacgaaggct ggcaaacgcc gtgcgcgaat
     1081 tcgcaaacgt attcacgcga attccaaacc acgccacgcc ataactgccc
//
LOCUS       DATCBB010000322         1770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_366569, whole genome shotgun sequence.
ACCESSION   DATCBB010000322 DATCBB010000000
VERSION     DATCBB010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366569"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VII37_03610"
     CDS             complement(<1..471)
                     /locus_tag="VII37_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY5068841.1"
                     /translation="MRFDLITIFPDFFTGPLDYGILRRARETGIVEVNVQDLREFTKD
                     RHRTVDDRPFGGGEGMVLKPEPLFEAVEHLLGAQVGDATQKRALPAGTAIVLLSASGT
                     RFDQETVRRFAKLQRIILICGRYEGVDERVAEHLATDEISIGDFVLSGGELPAML"
     gene            complement(485..1138)
                     /locus_tag="VII37_03615"
     CDS             complement(485..1138)
                     /locus_tag="VII37_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY5068842.1"
                     /translation="MSGQNPGWIVVARILRPRGNKGEVAVELLTDFPERLTKLKEVFL
                     GRVQGEAAAAKFAGAFGASEPSPIGVKSCWLSQNHRGQGVLHFSGVASINDAEKFRGL
                     DVLLPFEQRVSLPAGQYFVADLIGCAVFEADSGRPVVVSSSPCSMAVVPELLGTVRDV
                     QFPGEGESMRGTPLLEVETRQGELLIPLAEDICKRIDTVARRIEVTLPEGLRDLNSE"
     gene            complement(1135..1368)
                     /locus_tag="VII37_03620"
     CDS             complement(1135..1368)
                     /locus_tag="VII37_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY5068843.1"
                     /translation="MKELVETIARALVDRPEEVQVRTVGGAQVTVVELRVHAEDLGKV
                     IGRQGRTAKAIRTVLGAAGMKMRKRFTLEILED"
     gene            complement(1500..>1770)
                     /gene="rpsP"
                     /locus_tag="VII37_03625"
     CDS             complement(1500..>1770)
                     /gene="rpsP"
                     /locus_tag="VII37_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY5068844.1"
                     /translation="YHCWLRRRDLVLTIRLARIGKKKKPFYRVVVIERTRPRNGRVKE
                     LVGTYDPLKKPAEIKLDSERIKYWLGVGAQPSDTVRSFLKLQKIA"
BASE COUNT          419 a          605 c          385 g          361 t
ORIGIN      
        1 caacatcgct ggcagctcgc cgccgctcaa tacaaaatcc ccaatggaaa tctcatccgt
       61 agccaaatgt tccgccacgc gttcatcaac tccttcataa cgcccgcaaa tcaaaataat
      121 tcgctgcaac tttgcgaatc gccgcaccgt ctcctgatcg aatctcgtgc ccgaagccga
      181 tagcagcaca atcgcagtcc ctgcaggaag cgcgcgcttc tgagttgcgt cgccaacctg
      241 cgctcccaat aaatgttcca cagcctcaaa caacggctcc ggcttcaaca ccattccttc
      301 accgccgcca aacggccggt catccacggt ccgatggcga tcctttgtaa actcgcgcag
      361 gtcttgcacg ttcacttcaa cgattccggt ctctctcgcg cggcgcaaaa ttccgtaatc
      421 cagaggaccc gtaaaaaaat ccgggaaaat tgtaatgagg tcaaagcgca taggcgtgaa
      481 aagattactc cgaatttaaa tcgcgcaagc cttccgggag ggtgacctca atacgacgcg
      541 cgacggtatc aatgcgctta caaatatcct cagccagcgg aattagcaat tccccctgcc
      601 gggtctccac ttccaaaagc ggggtcccgc gcatactctc gccctcgccg ggaaactgca
      661 catcccgcac cgtccccaat aattccggca ctacagccat cgagcatggc gacgaggaaa
      721 ccaccacggg cctgccgctg tcggcctcaa acaccgcgca cccaatcaaa tctgccacaa
      781 aatattgtcc cgccggcaaa ctcactcgct gctcaaatgg cagcaacacg tccagcccgc
      841 gaaatttctc cgcatcattg atggaagcaa cgcccgaaaa atgaagcaca ccttgcccgc
      901 gatgattctg gctgagccaa caggatttta cgccgatagg ggatggttcg ctcgcgccaa
      961 acgcgcccgc gaattttgcc gcagccgctt cgccttgcac gcggccaaga aaaacttcct
     1021 ttaatttagt aagccgctcg gggaaatccg tgagcagctc taccgcgact tcgcctttat
     1081 tcccgcgcgg ccgcaaaatc cgtgccacca caatccatcc tggattctga ccgctcaatc
     1141 ttccaatatt tccagcgtaa accgcttccg catcttcatt cccgccgcgc ccaatactgt
     1201 gcgtatggcc ttggccgtcc gtccctgccg gccaatcact ttgcccagat cttccgcatg
     1261 gacccgcaac tccacgacag taacctgcgc tcccccgacc gtccgcacct gcacctcttc
     1321 gggacggtcc acgagggccc tggcgatggt ctctaccagt tccttcattg gctgctcctg
     1381 gatacatccg ccaccgaggg ggacagagtg acggcctacc aagtaccggt gtgtcttcta
     1441 accaagcaat tggttcggcg accaaaattg ccgtagaccc gtccgggtca aaaattcaac
     1501 tacgcaattt tctgcaactt caggaaactc cgcactgtat cgctgggctg cgcacccacg
     1561 cccagccaat acttgatgcg ctccgaatcc agcttaatct ccgcgggctt cttcaatggg
     1621 tcgtaagtgc ccaccaattc cttcacgcgg ccgttacgcg gacgggtgcg ttcaatcacc
     1681 accacgcggt aaaacggctt cttcttcttg ccaatccttg ccaaacgaat agtcaacact
     1741 aagtcacgcc tccgtaacca acaatgataa
//
LOCUS       DATCBB010000323         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_74552, whole genome shotgun sequence.
ACCESSION   DATCBB010000323 DATCBB010000000
VERSION     DATCBB010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74552"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VII37_03630"
     CDS             complement(<1..803)
                     /locus_tag="VII37_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5068845.1"
                     /translation="MRTLLLAVFVAVFTTGISAQKIPPPPEHRQKLNPDQVAQEVRDY
                     RMNNEDRIIRELSEFLAIPNIASDTENIQKNATQLVSMLEARGIETHLLPISGRGPVV
                     FGKLAVPNARRTLIFYAHYDGQPVDPAAWTDGKPFEPVLRTDAVENGGVRIPLPANSP
                     QHPAIYNDDWRIYARSSSDDKSPIVALLSALDALRANKIPLGINLKLILDGEEEDGSP
                     NLERVLLAHKNLLDVDVLITGDGPVHQSGRPLVFFGNRGILGFQITTYGA"
     gene            complement(858..1877)
                     /gene="trpD"
                     /locus_tag="VII37_03635"
     CDS             complement(858..1877)
                     /gene="trpD"
                     /locus_tag="VII37_03635"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY5068846.1"
                     /translation="MVPSLLQRVENGKILSRAQAEEAMEEILSGRAETPEIVSLLRAL
                     NARPIEPQELAGFASVMRRHAAPVFSDERARPAHLIDTCGTGGDSCETFNISTVAAIV
                     ASAAGASVAKHGNRAASSRCGSADVLEALGVRIDIPLQRCGEAIREIGLGFLFAPAAH
                     SATRHAALARKQIGERTVFNLLGPLTNPAGAHSQLVGVYSRDVLDLMTATLAELGVQH
                     ALVVHGADGLDEISLSGETYVAELHEGVVKRYTLTPEDFGMPRAPIETLRGGTAQENA
                     ALIREVLEARPGPRRDIVVINAAAALVACGIATNFPDGARLAANAISSGLAQKKLAAL
                     VAFTN"
     gene            1958..>2943
                     /gene="sucC"
                     /locus_tag="VII37_03640"
     CDS             1958..>2943
                     /gene="sucC"
                     /locus_tag="VII37_03640"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5068847.1"
                     /translation="MKIHEYQAKAILARYGVAIPRGEVAYRKEEARGTAQRLKSNIVV
                     VKAQIHAGGRGKGGGVKLARSADEAAEIASQILGMNLITAQTGPGGRIVKRLLIEEGL
                     DIKRELYLSLLVDRASGKVIFMASTAGGMEIEEVAKDNPEAILHEVISPVVGLQPYQA
                     RKLAFGLGLPIEAVNNAVPFLLALYKAFIDTDASMLEINPCVLTGDSKLVALDAKMTI
                     DDNALFRHKELRELRDLDEEDPLEVEASKYGLNYIKLDGSVACMVNGAGLAMATMDII
                     KYAGGSPANFLDVGGGASADQVKNAFGILMADPAVKAVFINIFGGILRCDVLA"
BASE COUNT          619 a          683 c          983 g          658 t
ORIGIN      
        1 gctccgtagg tggtgatctg aaatcccaga attccgcgat tgccgaagaa aaccaggggg
       61 cggccgcttt gatgcactgg gccatcgccg gtgatgagca catcgacgtc caggagattt
      121 ttgtgcgcga gtaagacgcg ctcgagattt ggcgagccgt cttcttcttc gccatcgagg
      181 attaatttca ggtttatgcc taggggaatt ttattggccc gaagggcgtc gagcgcgctg
      241 agcaaggcga ctatggggga tttgtcgtcc gaagaagaac gcgcgtagat gcgccagtcg
      301 tcgttataga tggcgggatg ctgggggctg ttggcgggta aaggaatgcg tactccgccg
      361 ttttccactg cgtcggttcg caagacaggc tcgaacggct ttccatcggt ccaggcggca
      421 ggatcgacgg gttgaccgtc atagtgagcg tagaaaatga gcgtgcggcg ggcgttgggt
      481 acggctagtt ttccgaagac aactggaccg cgtccggaga tgggaagtaa atgcgtttcg
      541 atgccgcggg cttcgagcat ggaaactaat tgagtggcgt ttttttgaat gttttcggtg
      601 tcgcttgcga tattaggaat ggcgagaaat tctgagagtt cgcggatgat gcgatcttcg
      661 ttgttcatgc gatagtcgcg gacttcctgg gcgacctggt ctggatttag tttttggcgg
      721 tgttcgggtg gcggtgggat tttttgcgca gaaatacccg tggtgaagac ggcgacgaat
      781 acggcgagca gtagagttct caaatttccc tcgatgacgc gatgttgcgc ggcaaattta
      841 tttcgtgcag tgaagagtta atttgtgaag gccaccaagg cggccaattt tttttgtgct
      901 aagcccgaag agatggcgtt agcggccaaa cgggcgccgt ctggaaaatt ggtagcgatt
      961 ccgcaggcga ccaaagcggc ggcggcgtta atgacgacaa tgtcgcggcg ggggcctggc
     1021 cgggcttcta acacttcgcg gatgagcgca gcgttttcct gggcggtgcc tccgcgcaag
     1081 gtttcgattg gcgcgcgcgg catgccgaaa tcttcgggcg tgagcgtata gcgcttcaca
     1141 actccttcgt gtagctcggc gacgtaggtt tcgccgctta gggagatttc atccaggccg
     1201 tctgctccgt gtacgacgag ggcgtgctgg acgccaagtt cggcgagcgt ggcggtcatc
     1261 aagtcgagta catcgcggga ataaacaccg acgagttggg agtgggcgcc cgctgggttt
     1321 gttaacgggc caagcaggtt gaaaacggtg cgttcgccaa tttgtttacg cgcgagggcg
     1381 gcgtggcgag ttgccgagtg agccgcggga gcgaaaagaa aacccaggcc tatctcgcga
     1441 atggcctcgc cgcaacgctg gagcggaata tcaatgcgca ctccgagggc ttccaatacg
     1501 tcggccgagc cacagcgaga actcgcggcg cgattaccat gtttggcgac gctggcgcct
     1561 gcggcggaag caacaatggc agcgacagtg gaaatgttga atgtttcaca gctgtcgccg
     1621 cccgttccgc aggtgtcgat caagtgcgcg gggcgcgcgc gttcgtcaga gaaaacgggg
     1681 gcagcgtggc ggcgcatcac acttgcaaag ccggcaagtt cttgcggctc gatggggcgc
     1741 gcattcaggg cgcggagcag acttacgatt tcgggagtct cggcgcggcc ggaaagtatt
     1801 tcttccatgg cctcttcggc ttgcgcgcgc gacaaaattt ttccgttttc gacgcgttgc
     1861 aacagcgaag gcaccatggt caccttgctc cgtctcggcc ttccggttaa actttactgt
     1921 ttcacgaacg caaaattcta tcactgatgg aacctagatg aagattcacg agtatcaagc
     1981 gaaagcgatt ctggcgcgtt atggcgtggc tattccgcgc ggggaagtgg cttatcgcaa
     2041 ggaagaagcg cgcggaacgg cgcagcgcct aaagtccaat atcgtggtgg tgaaggcgca
     2101 aatacacgcc ggcgggcgag gcaaaggtgg cggcgtgaaa ttggcacgct cggccgatga
     2161 agccgcggag attgccagcc aaattttggg gatgaactta atcacggcgc agaccgggcc
     2221 gggcgggcgg atcgtaaagc gtttgttgat cgaagagggc ctggacatca agcgcgagct
     2281 gtatctgagt ttactggtcg atcgcgccag cggaaaagtt attttcatgg ccagcacagc
     2341 gggcgggatg gaaatcgagg aagtggcgaa agataatccc gaagcaattt tgcacgaagt
     2401 aatttcgccg gtagtgggcc tgcagccgta tcaagcgcgg aagttagcgt ttgggttggg
     2461 cttgccgatt gaggcagtga acaatgcagt gccgtttttg ctggcgctgt ataaggcgtt
     2521 tatcgatacc gacgcttcca tgctggagat taatccgtgc gtgttgactg gagacagcaa
     2581 actggtggcg ctggacgcga agatgaccat cgatgacaac gcgctgtttc gccacaagga
     2641 attgcgcgaa ttgcgggatc tggatgaaga ggatccgttg gaagtggaag cttcaaagta
     2701 tggattgaat tacatcaagc tggatggcag cgtggcttgc atggtgaatg gcgcgggctt
     2761 ggccatggcc acgatggaca tcatcaaata cgctggcggg tcacccgcga actttctgga
     2821 tgtaggcggg ggtgcttcgg cggatcaagt gaagaacgca tttggaattt tgatggctga
     2881 tccggcagtg aaagcggttt ttattaatat ttttggcggg attttgcggt gcgatgtgct
     2941 ggc
//
LOCUS       DATCBB010000324         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_269837, whole genome shotgun sequence.
ACCESSION   DATCBB010000324 DATCBB010000000
VERSION     DATCBB010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269837"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /gene="rplP"
                     /locus_tag="VII37_03645"
     CDS             complement(<1..206)
                     /gene="rplP"
                     /locus_tag="VII37_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY5068848.1"
                     /translation="MLMPKKVKYRKQMRGRRTGKAWRGSDLAFGEYGLKALECAWITN
                     RQIEASRVAISRFIKRGGKLWIRI"
     gene            complement(266..943)
                     /gene="rpsC"
                     /locus_tag="VII37_03650"
     CDS             complement(266..943)
                     /gene="rpsC"
                     /locus_tag="VII37_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY5068849.1"
                     /translation="MGQKTHPYGFRLGYNKPWKSRWYADRDYAELLHEDVKLRAELKE
                     KLKSAGISSIDIERAANKLVVRIYTARPGIIIGRKGAEIDKLKQDVQKRTKREVHIDI
                     QEVHRPELDAQLVAESIALQLEKRVAFRRAMRKAVDSALRFGCKGIKVRVAGRLNGAE
                     IARKEWYLQGRLPLQTLRADIDFGMARANTTYGVIGVKCWVYQGENVPKRASKVQKER
                     EAVPALA"
     gene            complement(1142..1477)
                     /gene="rplV"
                     /locus_tag="VII37_03655"
                     /pseudo
     CDS             complement(1142..1477)
                     /gene="rplV"
                     /locus_tag="VII37_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(1511..1849)
                     /gene="rpsS"
                     /locus_tag="VII37_03660"
     CDS             complement(1511..1849)
                     /gene="rpsS"
                     /locus_tag="VII37_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY5068850.1"
                     /translation="MGRSLKKGPFVDGHLREKVEGLNTRNEKKVIKTWSRRSTIVPDM
                     IGHTIAVHNGKKFIPVYVTEQMIGHKLGEFSPTRTFKGHGVKAALERAAGPAPAGGGA
                     GAPAAAAPRS"
     gene            complement(1857..>2100)
                     /locus_tag="VII37_03665"
     CDS             complement(1857..>2100)
                     /locus_tag="VII37_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068851.1"
                     /translation="PKVVAKPVRASLKPVIVPPEPVLYKLCKPGWGVIVERIPNAQGT
                     RFVCPFCPRSHRVPGPVRGFKKMRRAQWSRLRRVEE"
BASE COUNT          365 a          686 c          534 g          515 t
ORIGIN      
        1 aaaatccgga tccaaagttt tccgccgcgc ttgatgaagc gcgaaatcgc cacacgcgaa
       61 gcttcaatct ggcggttggt aatccacgca cactccagcg ccttcaaacc gtattcgcca
      121 aatgccagat cgctgccgcg ccaggctttg ccagtgcgcc gcccgcgcat ttgctttctg
      181 tacttcacct tcttgggcat caacatcgct atgttctcgc ttccctaatc tcgtttaatt
      241 cttgcttgca ccgttcggcc gcaaactacg ctaatgcggg gacggcctcg cgttcttttt
      301 gtactttcga agcgcgcttt ggaacgtttt cgccctgata aacccagcac ttcacgccaa
      361 tcacgccgta agtggtattt gcgcgcgcca tgccgaaatc gatgtccgct cgcagcgttt
      421 gcagcggcaa gcggccctgc aggtaccact ctttacgcgc gatttccgcg ccattcaagc
      481 ggcctgctac gcgaaccttg atgcctttgc aaccgaaacg cagcgccgag tccactgctt
      541 tgcgcatggc gcggcgaaaa gctacgcgct tttcaagctg cagcgcgatg gattcggcca
      601 ccaactgtgc atcgagctcc gggcgatgca cttcctgaat atcgatatgc acttcacgct
      661 tggtgcgctt ttgcacgtct tgcttcagct tgtcgatctc cgcgcccttg cgcccgatga
      721 taatgcccgg acgcgcggtg tagatgcgca ccaccagctt gttcgcggcg cgctcgatat
      781 cgatagagct tatgcccgcg ctcttcagct tttctttcaa ctccgcgcgc agcttcacgt
      841 cttcgtgaag cagttcagcg tagtcgcggt cagcgtacca gcgagacttc cacggcttgt
      901 tgtagcccag tcgaaatccg tacgggtgtg tcttctgtcc cattcttatc ctcttcgtcc
      961 aaaaactcgt gccatgtccg gagcgaccac gttacttctt cttgcccttt ttcggatgcg
     1021 ccccttgctt gccggttaat gctttgcgaa ctcggcgagc tgtgccgcgc acaccctttt
     1081 gcgcttcggt ttccgcttca atggcggcca cgcgttctgc tgcggccaca tgatgctccg
     1141 ccacgcccac ccacagatgc gccgtgcgct tctgatagcg aaatgcgcgg cccatcggcg
     1201 cgggacgcaa acgcttccag cgcgtgcctt cgttcacgta gcaggtgttt acgtaaagct
     1261 catccacgtc cagcggcgcg ccagcatctt ccgctttgcg ttcggcattc gcgatggcgc
     1321 tgcgcagaac tttttcaata tgcttggcgg aacgcttttg cgtgaaacgc aaagtgtgca
     1381 gcgcgtcctg agccttttgc ccacgaatca aatccataac cagccgcgct ttttgcggcg
     1441 acatacgcac atgcttggcc agcgcgtggg cttcgatgtt cgcttgcgtc tgttgtagtt
     1501 cagccatgtc ttaagacctc ggcgccgctg ctgctggtgc tcctgcgccg cctcctgctg
     1561 gtgccggtcc agcagcgcgt tcgagcgccg ccttcacgcc gtgaccttta aaggtgcgcg
     1621 tgggagaaaa ttcacccagc ttgtgcccaa tcatctgttc cgtgacatac acgggaataa
     1681 atttctttcc gttatgcacc gcgatggtgt ggccaatcat gtccggcacg atcgtcgagc
     1741 gccgcgacca cgtcttgatt acttttttct cgttacgcgt gttcaggcct tcaacctttt
     1801 cgcgcaaatg cccgtccacg aacggtccct tttttaatga gcgtcccatc gatcccctat
     1861 tcctctactc gacgcaagcg gctccactga gctcgccgca ttttcttgaa tccccgcact
     1921 ggccccggca cacgatgcga cctgggacag aaggggcaaa cgaagcgcgt gccttgcgcg
     1981 ttcggaatgc gctccacaat cacaccccat ccgggcttgc acagcttgta cagcaccggc
     2041 tccggcggaa cgattaccgg cttcaggctc gcgcgcaccg gcttggctac tacctttggc
//
LOCUS       DATCBB010000325         1481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_464514, whole genome shotgun sequence.
ACCESSION   DATCBB010000325 DATCBB010000000
VERSION     DATCBB010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464514"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VII37_03670"
     CDS             <1..630
                     /locus_tag="VII37_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5068852.1"
                     /translation="WKRRVFKRKADQVWFPGESISMGIGQGPITVTPLQQAHFAAEIA
                     ERGKVIAMPRLVSGTRAPGSVIVVPRPSTFAKPVDMATDEQWNVIYDGMIGAVTQPGA
                     TAYLAFRGALYKLAGKTGTAQVVTVKQTESTKHQDADERKREHAWFIAFAPVEDPKIA
                     ISVLIENGGFGGSTAGPIARKVLDTYLLADAGAEPKKNSAELGSATNSH"
     gene            complement(1178..>1481)
                     /locus_tag="VII37_03675"
     CDS             complement(1178..>1481)
                     /locus_tag="VII37_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068853.1"
                     /translation="NATWRDPRGPYARLDVTGMGPFFYDLPPNWTRSSAYGLVNGKLG
                     WETSRWEAYFWGRNLLDKNYTVRGFYFGDEPPDFPIKLYTQLGEPRNWGVHFTIRY"
BASE COUNT          360 a          417 c          395 g          309 t
ORIGIN      
        1 tggaaacgac gggtgttcaa gcgcaaagct gatcaagtgt ggtttcccgg cgaaagcatc
       61 agcatgggca tcggtcaggg cccgatcacg gtcacacctt tgcagcaggc gcattttgcg
      121 gcagaaattg ccgagcgcgg aaaagtcatc gcgatgccgc gcctggtctc gggcactcgc
      181 gcccccggat cggtgatcgt ggtaccacgc cccagtacct tcgccaaacc ggtcgacatg
      241 gctaccgatg agcagtggaa cgtgatttac gacgggatga tcggcgccgt cacgcaaccg
      301 ggcgcgactg cttacctagc ctttcgcggc gcgctctaca agctcgcagg caaaacggga
      361 accgctcagg tagtaacggt caagcaaacc gaaagcacca aacaccagga tgcggacgag
      421 agaaaacgcg agcacgcatg gtttatcgca tttgcgccgg tcgaggatcc gaagattgcg
      481 atttccgtat taattgagaa cggcggcttc ggtggatcga ccgccggtcc cattgctcgc
      541 aaggtgctcg acacgtacct gctggctgac gcgggggccg agccgaaaaa aaactctgcc
      601 gagcttggca gcgcaactaa ttcgcattag aaccctttca caaactgttt cgtttccttg
      661 gcggcctctt tccatatcgc tttatgtttg ttgaaaagcg caacaagtcc agcggcttcg
      721 agcctttgct cagcttctaa tgtcagtgcc attattttat atccatggct atacgtcctg
      781 cagcgacgag taccagtatc aacgcaaata gcaaagcgac aatggccgct aaagcggcac
      841 attcgattcc tatccattta ccaacgagtg ccattgcgcc ggataaaata gagagcgcaa
      901 agctcacaaa agcaaaccac gcacggcgtc taaactttcc gtgaacggct acttgttctt
      961 tttttgataa gagtcgaacg ctgtcgatac tgcgccgagg tagacgagta gcaggcccgc
     1021 cagcgcgatg gatgctgctg ccacatcacc agctacgcta agagccccaa cattccctgt
     1081 tgcctccata tgtttcgata ttcgtccaat cgccaaaaca acgcaagata atctggctga
     1141 cgcaaagcta gatcaagtgt gaatcgagaa ttgggtgcta atagcgaatg gtgaaatgca
     1201 cgccccaatt tctgggttcg cccagctggg tatacagttt gatgggaaaa tccggcggtt
     1261 cgtcgccaaa gtaaaatccg cgcaccgtgt agtttttatc tagcaggttg cgtccccaaa
     1321 aataagcttc ccagcgggaa gtttcccaac ccagcttgcc gttgaccaat ccataggcgc
     1381 tcgagcgggt ccagttcggc ggcagatcgt aaaaaaacgg gcccatgccg gtcacgtcca
     1441 gccgcgcata cggtccacgc gggtcgcgcc aggtcgcgtt g
//
LOCUS       DATCBB010000326         1023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_269935, whole genome shotgun sequence.
ACCESSION   DATCBB010000326 DATCBB010000000
VERSION     DATCBB010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269935"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..>1023
                     /locus_tag="VII37_03680"
     CDS             373..>1023
                     /locus_tag="VII37_03680"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068854.1"
                     /translation="MTKEEILAAIRRAAEELGRAPSRGELRRITGVSHYRVLAEFPTL
                     REAVRAAGLEPNPKGEKISTEDLLTDWKRVAEKLGRRPSRAEYVREGKYSAGALTVRF
                     GSWSGISTAKGAKGAKERKAVHRGGAETRRKKELPKLPELPRLPKVEERNHKGHPHDS
                     SFASSESLRAGYGSQRESGGLAQSARVVKLGESVLHNFDCAPVAQLPAPLVGMRRVT"
BASE COUNT          271 a          226 c          361 g          165 t
ORIGIN      
        1 gaaaaacccg gctggcaggg tatggacacg gggacggaga cggcgccgag gcatacgagc
       61 aggtagagca gccattagca cttagcaaaa acaacaaagg catcatcgct cccgaggaaa
      121 ctcgggagct tttttggcga agagagccat tagcagttag cacttagcca ttagtaaaac
      181 caaacccaaa gaccaaaggc aaaaccaaag gcaaacctta ccgcggattg cgcggataaa
      241 cgcggatttg cacggatttt tttggttgtg ttttggatga gtttcgctta aaattcgctt
      301 gagctaaaac aggcactctc ggtgaagagt tcgagcattg tctgagaagt gcgatcgaag
      361 gcgaaagatg cgatgacgaa agaagagata ctggcggcga tacggcgggc ggcggaggaa
      421 ctgggaagag cgccgtcgcg cggggagctg cgtcggatta cgggagtgag ccattaccga
      481 gtgctggcgg agttcccgac gctgcgggaa gcggtgcgag cggcggggct ggagccgaat
      541 ccgaaaggcg agaagatcag cacggaagat ttgctgacgg actggaagag ggtggcggag
      601 aagctgggaa gaaggccgtc gcgggcggag tatgtgcgtg agggaaagta cagtgcgggg
      661 gcgttgacgg tgcggtttgg atcgtggtcg ggaatctcaa ccgcaaaggg cgcaaagggc
      721 gcgaaggaaa gaaaggcggt tcaccgcgga ggcgcggaga cgcggagaaa gaaagaattg
      781 ccaaaattgc cagaactgcc aagattgcca aaagtggaag agcgtaacca caaaggacac
      841 ccgcacgact cctctttcgc aagctcagag tcgctcaggg caggctacgg atcacaaagg
      901 gagagcggcg gattggcgca atctgcgcga gtcgtgaagc ttggcgaatc tgtgctgcac
      961 aactttgatt gcgcgccagt ggcgcagctg ccggcgccgc tggtggggat gcgtcgagtg
     1021 acg
//
LOCUS       DATCBB010000327         1329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_366795, whole genome shotgun sequence.
ACCESSION   DATCBB010000327 DATCBB010000000
VERSION     DATCBB010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366795"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1329
                     /locus_tag="VII37_03685"
     CDS             <1..>1329
                     /locus_tag="VII37_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5068855.1"
                     /translation="LGWHGREEKSISFRPPDEVLLTPAQRSCAWFFLVMALLFLMQAL
                     LGGAGEHYRAELSNFFGFDLGRVLPFNIARTWHLQLAIFWVSTSYLAAGIFIVPMITG
                     REPRGQGVLSYLLLGALAIVVFGSMGGEYAGVFGWIQNGWSWFGHQGFTFLDLGRFWQ
                     ILLTVGLFFWVVILFRGLRKRLRNEYMGNLPWLFFFAALAIPAFYAVGLLVRNDSTFT
                     TTDFWRFWVVHLWVEDFLELFTTVMVAYIFVLLGVVNQRVAMRLIYLDVILYSAGGVI
                     GTMHHLYFSGEPALHMALGAFFSAAEVIPLTFLTVEAWSFLQLGAQQSARTRTPFPHF
                     WSVMFLASVGFWNFLGAGIFGFLINLPIVSYYEIGTALTANHAHAAMMGVYGMLAVGL
                     ALFCLRYLIPEKLWSDRAAKISFWSLNLGLAWMVFATLFPLGCIQLYHSLN"
BASE COUNT          187 a          387 c          398 g          357 t
ORIGIN      
        1 ctcggctggc acggtcgtga ggagaaaagc atttcgttcc gaccgccgga tgaagtgctg
       61 ctcacgccgg cacaacggtc atgcgcctgg ttcttcctcg tcatggcgct gctgtttctg
      121 atgcaggcgc tgctgggcgg cgcgggcgaa cattatcgcg cggagctgtc caactttttc
      181 ggcttcgatc tggggcgcgt gctgccgttt aacatcgccc gcacctggca tttgcaactg
      241 gcgattttct gggtctccac gtcctatctg gcggcaggca tttttatcgt gccgatgatc
      301 accggccgtg aaccgcgcgg ccagggcgtg ctttcctatc tgctgctcgg cgcgctggca
      361 atcgtggtgt tcggcagcat gggcggcgaa tatgcgggcg tcttcggctg gattcaaaac
      421 ggctggtcat ggttcggcca tcagggattc acctttcttg atctcggccg gttctggcaa
      481 atcttgctga cggtggggct gttcttctgg gtcgtaatcc tgttccgtgg cttgcggaag
      541 cggttgcgca atgaatacat gggcaacctg ccgtggctgt tcttttttgc cgcgctcgcc
      601 attcccgcct tttacgccgt cggactgctc gtgcggaatg attccacgtt caccaccacg
      661 gatttctggc ggttctgggt cgtgcatctc tgggtcgagg attttctcga attgttcacg
      721 acggtgatgg ttgcctatat tttcgtgctg ctcggcgtgg tgaaccagcg cgtggccatg
      781 cggctgattt atctggacgt gattttgtat tcggcgggcg gcgtcatcgg cacgatgcac
      841 catctgtatt tctccggcga acccgccctg cacatggcgc tcggcgcgtt cttctcggcg
      901 gcggaagtga ttccgctgac cttcctcacg gtcgaggcct ggagcttcct ccagctcggc
      961 gcgcaacaga gcgcacgcac gcgcacgccg ttcccgcatt tctggtcggt gatgtttctg
     1021 gcgtccgtcg gcttctggaa ttttcttggc gcgggcattt tcggttttct catcaacctg
     1081 ccgattgttt cctattatga aatcggcacc gcgctcacgg ccaatcacgc ccacgcggca
     1141 atgatgggcg tttacggaat gctggccgtc ggcctcgcgc tcttctgcct gcgctacctg
     1201 attcccgaaa aattgtggag cgatcgcgcc gcgaaaatca gtttctggtc gctgaatctc
     1261 ggtctggcgt ggatggtttt tgccacgctg tttccgctcg gttgtatcca gctctaccac
     1321 tcgctgaat
//
LOCUS       DATCBB010000328         1153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_464616, whole genome shotgun sequence.
ACCESSION   DATCBB010000328 DATCBB010000000
VERSION     DATCBB010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464616"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1153)
                     /locus_tag="VII37_03690"
     CDS             complement(<1..>1153)
                     /locus_tag="VII37_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5068856.1"
                     /translation="ERLSYYAAFASLARYLHETLSFPAAQASSLTGLFGGLVWFLAAF
                     GGTLADRLGFRRSLSLAYFILSGSYFLLGSLGSSWLAPVRAAVPLWLLVGFVLMLPAL
                     GIALVKPCVVGTTARASKENVRSIGYSIYYTLVNIGGAAGPYVASWVHQHMRVESVFR
                     VAALSVFLMFFAVLLLFKEPRRSNEVQTASSGAAWRNFVMVLSNPQFMIFLLIFSGYW
                     IVYWQEFIILPIYMHDYVNSSTDTELMLVTGPLTVIALQLLMSFLMKKVAAFRAITLG
                     TLVSGLAWLILIVHPTPAMAIATLFAVSLGEIIQSPRYYEYISRLAPAGQQGTYMGFA
                     FLPIGIGSLIAGPLGGLLLHYFGEVQHQPQRIWWAVTGIGVLTALLLYAY"
BASE COUNT          331 a          333 c          282 g          207 t
ORIGIN      
        1 catatgcata aagcaatagt gcagtgagaa cgccaatccc tgtcaccgcc caccagattc
       61 gttgcggttg atgctgcact tcgccaaaat agtgaagcag tagccctccg agcggcccag
      121 cgatcaacga tccgataccg atcggtaaga acgcaaatcc catatacgtt ccctgctggc
      181 ccgcaggcgc aagccgcgag atatattcgt aatatcgcgg cgattggata atttcaccca
      241 acgaaactgc aaaaagagtg gcgatcgcca tcgccggcgt gggatgcaca atcaggatca
      301 gccacgccaa tccagaaacc aatgtcccca gggtgatggc tcggaaggcg gcgacttttt
      361 tcatcaaaaa actcatcaat agttgcaaag caatcaccgt cagcggcccg gtgaccaaca
      421 tcaactcggt atcggtactg gaattcacgt aatcgtgcat ataaatcggc aaaattatga
      481 actcctgcca atacacgatc cagtagccgg aaaaaatcaa aagaaaaatc atgaattgtg
      541 gattgcttag caccatcaca aaatttcgcc acgctgcgcc cgagctcgcg gtttgtactt
      601 cattcgatcg tcgaggttct ttgaacagca atagaaccgc aaaaaacatt agaaatacgc
      661 ttaatgcggc cacgcgaaaa acactttcaa cccgcatatg ctggtgcacc caggacgcta
      721 cgtacggtcc cgcggctccg ccgatattca cgagcgtgta gtaaatcgag tagccgatgg
      781 agcggacgtt ctcttttgac gcgcgcgccg tagttcccac cacgcagggc ttgaccaaag
      841 caattcccag cgccggaagc atcagtacga acccaaccag caaccataac ggcaccgcag
      901 cgcgcaccgg cgcgagccac gaggaaccta gcgatcccag cagaaagtag gacccgctta
      961 aaatgaaata cgcgagcgag agtgatcgtc ggaagcccag gcgatccgct aacgttccgc
     1021 caaatgcggc gagaaaccat accaggccgc caaacaatcc ggtcaggctg ctggcttgcg
     1081 cggctggaaa actcagcgtc tcgtgcaaat aacgcgccag cgatgcaaac gccgcatagt
     1141 aggaaaggcg ttc
//
LOCUS       DATCBB010000329         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_204931, whole genome shotgun sequence.
ACCESSION   DATCBB010000329 DATCBB010000000
VERSION     DATCBB010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204931"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VII37_03695"
     CDS             <1..384
                     /locus_tag="VII37_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068857.1"
                     /translation="VPALYACQPGLDIINKVGVRAIREKSLRMTARILAGAKSRGWAV
                     NTPENEAERAGTVSVECPHAAEVCQELVKRNILVDYRPKAGVRVSPHFYNTESECDFT
                     LKQMDEIVATLDQQKFGTKVSQDSR"
     gene            384..1145
                     /locus_tag="VII37_03700"
     CDS             384..1145
                     /locus_tag="VII37_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEY5068858.1"
                     /translation="MNASNFDLSIIIPSYNEEARLPETLKRIAEYLPTLGLRTEVLVV
                     DDGSTDRTAAVAESFLGKLTGLRVLSNGTNRGKGFSVRHGMLEAQGDMVLFTDADLSA
                     PIEESDKLLAALNNGYDVAIGSRAMDRGLISTHQSIFREFAGIIFNKIVRIVLRLPFV
                     DTQCGFKAFRRERCGIIFEQQRIEGFGFDPELLYLARHHGLRAIEIPVRWGHSAATKV
                     SMLGDSLKMFADIFTIRWNAMSGRYARSGRRVEAV"
     gene            1245..1859
                     /locus_tag="VII37_03705"
     CDS             1245..1859
                     /locus_tag="VII37_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068859.1"
                     /translation="MLKTSSVPTPRTGPIPIDARAADHLRYIRETMESAAEFTAVPGW
                     GGVAMGVTAVVAAKVAAMQTTPRAWFGVWLVEAFIAVAIAAPAAATKAHRANSKLFSG
                     PGRKFLLSFAPPIIVGGLMTFAFYSSGSFAILPAVWLLLYGAAVVTGGAFSVRVVPVM
                     GLCLMGLGGCALFAPAAWGNYFMAAGFGVVQIAFGLWIARHYGG"
     gene            1864..2256
                     /locus_tag="VII37_03710"
     CDS             1864..2256
                     /locus_tag="VII37_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY5068860.1"
                     /translation="MPKSSAAPQDRIPRARQREDSPSTVREQTREAVSPDLDRLIHER
                     IRLGIVSALAVNRSLTFNELKALLKTTDGNLSVHARKLEEASYIMCSKSFDGRLPKTE
                     YRLAGAGRKALERYLNHMEALIRATREK"
     gene            2348..>2716
                     /locus_tag="VII37_03715"
     CDS             2348..>2716
                     /locus_tag="VII37_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEY5068861.1"
                     /translation="MPSLTSIEAGSNTRPASSGIHVAIISDGNGRWASAKGLPRSAGH
                     RAGAETAREIIEAAPSLGIHTLTLFALSSANWQRPTSEIAGILRLLHEYLLTETSHCM
                     EEGVRLTVMGRRDRLPANLRQ"
BASE COUNT          578 a          714 c          817 g          607 t
ORIGIN      
        1 gttccggcac tctatgcttg ccaaccggga ctggacatta ttaataaggt gggcgttcgc
       61 gccattcgag aaaagtcact gcgcatgacc gctcggattc tggcgggcgc aaaatcgcgc
      121 ggatgggctg tgaatacgcc ggaaaatgaa gcggagcgcg ctggaacggt atctgtggaa
      181 tgtccacatg cggccgaagt gtgccaggaa ttggtgaagc gaaatatcct cgtcgattac
      241 cggccaaaag cgggagtacg cgtttcgccg catttctaca acacggaaag cgaatgcgat
      301 tttacgctta agcaaatgga cgagattgtt gcgactcttg accagcagaa gttcggaacg
      361 aaggtctcgc aggattcgag ataatgaacg catccaattt cgatctgtcc attattattc
      421 cctcctataa cgaagaggcg cgcctgcccg agaccttgaa acgtattgct gaatatcttc
      481 cgacgctggg attgcgcacc gaggtgctgg tcgtcgacga tggctcgaca gatcgaaccg
      541 cggcggtggc ggaatcattt ctcgggaagc ttaccggact tcgcgtgctt tcgaacggca
      601 cgaatcgcgg aaaagggttc agcgtgcggc acgggatgct ggaggcgcag ggcgacatgg
      661 tgctctttac ggacgccgat ctttccgcgc ctatcgagga gtccgacaag ctgctagcgg
      721 cgctaaataa tggctacgat gtggccatcg gatcgcgagc gatggaccgc ggcttaatca
      781 gcacccacca atcaattttt cgggagttcg cgggcattat ctttaataaa attgtgcgca
      841 tcgtcttgcg cttgccattt gtggatacgc aatgcggctt caaggctttc cggcgcgagc
      901 gctgcgggat tattttcgag caacagcgca ttgaaggatt cggatttgat ccggaattgc
      961 tatacctggc gaggcatcac ggattgcgag ccatcgaaat tccggttcgg tggggccact
     1021 ccgccgcgac aaaagtcagc atgctgggag acagtctcaa aatgttcgcg gacatattca
     1081 cgattcggtg gaacgcgatg agcgggcggt atgcccggag cggccggagg gtcgaggctg
     1141 tctgaatgta ctttgtggcg taaagtactt gacagcgaat gatgcgtctg ctagttttct
     1201 tgcgacgtgt tgacgcggtc agaattgcct aggttgcatt cgcaatgttg aaaaccagct
     1261 cggtccccac tcccagaacc ggccccattc ccatcgacgc gcgggctgcc gaccatcttc
     1321 gctatatacg tgaaaccatg gaatcggcgg cggaatttac cgccgtgccc ggctggggcg
     1381 gcgtggctat gggggtcacc gcagtggttg ccgccaaggt cgctgccatg cagaccactc
     1441 cgcgcgcctg gtttggcgta tggctggtgg aagcctttat cgcggtggcc atagccgccc
     1501 cggccgcagc taccaaagcg catcgcgcca attcaaaact tttctccggt cctggccgaa
     1561 aatttcttct cagctttgcg cctccaatca tcgttggagg cctgatgaca tttgcgtttt
     1621 acagttcagg atcatttgcg attttgccgg cagtgtggct gttgctgtac ggcgcggcgg
     1681 ttgtgactgg cggggctttt tctgtgcggg tcgtaccggt gatgggactg tgcctgatgg
     1741 gcctgggcgg atgcgctttg ttcgcaccag cagcctgggg aaattacttc atggccgcgg
     1801 gttttggcgt ggtgcaaatc gccttcggcc tatggatcgc ccggcactac ggaggataac
     1861 gatatgccta aatcttctgc agctcctcaa gaccgcattc cacgtgccag gcagcgcgag
     1921 gattcgccgt ccaccgtgcg ggagcaaacg cgcgaggcgg tttcgccgga tctcgaccgc
     1981 ctgattcacg aacgcatccg actgggcatc gtaagcgcgc tggcggtgaa ccggtcgttg
     2041 accttcaatg aattgaaagc gcttttgaag accacggatg gaaatttaag tgtgcatgca
     2101 cgtaagttgg aagaggccag ctatatcatg tgcagcaaat ccttcgatgg gcgcttgcct
     2161 aagacggaat accggctggc tggggccggg cgcaaagcgc tggaacgcta cctcaatcac
     2221 atggaagcat tgatccgggc cacgcgggag aaataagcgc ggcaaatcac tgaaatacga
     2281 tctccttgtg tctagttgct ttgtgttaca gagtagtttt caataataaa atattaacga
     2341 ggtacgaatg ccttctttaa cttcgatcga agctggttcc aacacccggc cggccagcag
     2401 cggcatccac gtggccatca tctcagatgg caacggccga tgggctagcg ccaaggggct
     2461 tcctcgatcc gccggccatc gcgcgggagc ggaaaccgcg cgagagatta ttgaagcagc
     2521 acccagtttg gggattcaca ctttgacgtt gttcgcgctg tcttccgcga actggcagag
     2581 gcccacttcg gagatcgcag ggatactgag gctgctgcac gaatatttgc ttaccgagac
     2641 atcccactgc atggaagaag gggtgcgctt aacagtgatg ggacgccgcg accgtttgcc
     2701 ggccaacttg cgtcag
//
LOCUS       DATCBB010000330         1429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_399927, whole genome shotgun sequence.
ACCESSION   DATCBB010000330 DATCBB010000000
VERSION     DATCBB010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399927"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..>1429
                     /locus_tag="VII37_03720"
     CDS             209..>1429
                     /locus_tag="VII37_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEY5068862.1"
                     /translation="MSTVPATSRANLQLALGTVSFAICFAAWGLISAFAPRFRETFHL
                     TATQTAFLVAVPVLLGALLRIVTGMLADRFGGRAIFSLLLIVVAVPAYLVPVVSSYKL
                     LLYVAFFLGMAGSSFAVGVGFVSRWFPPGKQGGSLGIYGLGNIGQSAAVFLGPVLAVA
                     VGWQNIFRGMAALLVIWGMAFHFLARNSPKTVRPSGIGAMLQLLSRERLSWVLSLFYF
                     LTFGGFVAFSIYLPVLFKDQFGLKLADAGFRTAGFVVLATLARPLGGWLADKIGGARV
                     LHVVFLGIIPFALLMSWPAMLPFTVGALGCAFLLGNGNGAVFKLVPQYFPKEVGTVTG
                     LVGAMGGLGGFFPPLLLGFFRDHFHTTWPGFALLAFLSALVWRINAVVFLSKQQTVEL
                     TLPARLTRTADRVRA"
BASE COUNT          210 a          397 c          428 g          394 t
ORIGIN      
        1 tctggagccc agccactcgc agaaacaggc catggttgtg ggggttccag taaagatttc
       61 ttaaagcgta ccaggaacta ggatagtaac tgctagtacg tttcagtttg tcggaagttc
      121 aatgtaagag gtatgctgga cctcccttcc gatcagtacc tttctaaccg gcgcgtccaa
      181 cctgccggta tcattgcgga gcaaacggat gagcacggtc ccggccacct ctcgggctaa
      241 tttgcagctg gctcttggta cggtgtcttt tgcgatctgt tttgccgcgt ggggattgat
      301 cagcgccttc gcgccgcgat tccgcgaaac tttccactta actgccacgc agaccgcctt
      361 cctcgtagcg gtgcctgtgc tccttggcgc gctgctgcgt atcgtgacgg gaatgctggc
      421 ggatcgcttt ggtggacggg caattttctc gcttctgctg atcgttgtag ccgtgcccgc
      481 gtatctggtg ccggtggtct cgagttacaa gctgctgctg tacgttgctt ttttccttgg
      541 catggccgga agttcgttcg cggttggtgt gggatttgtc tcccgctggt ttcccccggg
      601 aaaacaaggt ggctcgctgg gaatttatgg gctcggcaat atcgggcaat ccgctgccgt
      661 tttccttggg cccgtccttg cggtagcggt cgggtggcag aatatttttc gcggcatggc
      721 ggcgctgctc gtcatttggg gcatggcctt ccacttcctt gcccggaact cacccaagac
      781 ggttcgccct tccggtatcg gcgcgatgct gcagcttctt tcgcgcgagc gattgtcctg
      841 ggtgctctcg cttttttact ttctcacgtt cggcggtttc gtggcgtttt ccatctactt
      901 gcccgtcttg ttcaaagatc agttcggcct gaaactggct gacgccggat ttcggacagc
      961 gggctttgtt gtgctggcga cgctggcgcg tccgctcggc gggtggctcg ccgataagat
     1021 aggcggcgcc cgcgttcttc acgttgtgtt tctcggaatt attccgttcg cgctactaat
     1081 gtcctggccc gcgatgttgc catttacggt tggcgctctc ggctgtgcct tcttgcttgg
     1141 aaacggcaac ggcgctgtct ttaagctcgt gccgcaatac tttccgaaag aagtcgggac
     1201 ggtaacgggg ctggtgggag cgatgggcgg gctcggcgga ttctttccgc cactgcttct
     1261 cggttttttc cgggatcatt ttcacactac gtggccaggc tttgcgctcc tggcatttct
     1321 ttccgcgttg gtttggcgta tcaacgccgt ggttttcctg agcaagcagc agacagtgga
     1381 actgacactt cccgcccgtc tcacgcggac ggcagaccgc gtaagagcg
//
LOCUS       DATCBB010000331         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_236717, whole genome shotgun sequence.
ACCESSION   DATCBB010000331 DATCBB010000000
VERSION     DATCBB010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236717"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VII37_03725"
     CDS             <1..547
                     /locus_tag="VII37_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5068863.1"
                     /translation="EGMGLFDRLTGTDYLRFVGRMYGLDQPTTDRRAEELLEFMQLAD
                     RPKTMIVDYSHGMQKKLALAAAVIHGPRVLFLDEPFEGVDALAAGALKSLLGRMTERG
                     VTIFLTSHVMEIVERLCSHVAIIDKGRLVAQGSLEELRAGVPGDEGGKVTLEQIFLSI
                     VGQHGGQRGVESQVDELSWLT"
     gene            535..2214
                     /locus_tag="VII37_03730"
     CDS             535..2214
                     /locus_tag="VII37_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068864.1"
                     /translation="MADLAGPISLIDQIRLVAGLRWRMAINSLRKKNNILDLIGMGFV
                     SLAALILIIGPCFGFYFAGYSLISAGRLQWLAAPFWAIFIFWQVIPIFAAGFGASFEF
                     RTLLRFPFSASAFYLIGLAYGLANFPTIACICWLLLMTAGAAVANPSVLPAMLVVVIL
                     VILLNVTLERLIGSWLERLLARRRSREIFLGVFILLMFSMQFIMPMQRQYAKRNSNPA
                     VFVGMVKYLAPFPPSLSARVLVGAVRHDFVDIAVGSSGLLGFVFLFSALLWQRYAAQY
                     RGEELSESPAPSPAASVRATRWRSIPQITSTGRISPGLLPPAIGPVVRKEFSYLARNG
                     FAFFLLVLPPAQILLFGSQFGGKHLAIAGRGLNADLFFPAMMAYTVLVTMGPAYNAFA
                     YEGRGIQTYFTAPLRFSDLLAGKNLVSAATVGFEVALCGVVLAWAFGPPSIPTLFATI
                     FALIFTVSGQLPIANWASLSFPRKLEFGSMRSQRNSGVSVWIMFGAQIVLAGISALVL
                     SSARWSGNPWLPAEGFAFLAAAALGGYFASLQALSELAEKKKEILIEALCR"
BASE COUNT          447 a          585 c          656 g          616 t
ORIGIN      
        1 cgaaggcatg ggattatttg accgcttgac cggcacggac tatctgcgat ttgtgggacg
       61 catgtatggc ttagaccagc cgacgacgga ccggcgcgcc gaagagttgc ttgagtttat
      121 gcaactggca gaccgcccga aaaccatgat cgtggactac tctcacggaa tgcaaaaaaa
      181 actcgcgctg gccgccgcgg tgattcacgg accacgcgtt ttgtttctgg acgaaccgtt
      241 cgaaggcgtg gacgcgctcg ccgcgggtgc cctgaaatcg cttttgggaa gaatgacgga
      301 acgcggcgtc acgatttttc tgacctctca cgtcatggaa attgtggagc gcttatgtag
      361 tcacgtggcc atcattgaca aaggccgctt ggtggcccaa ggctcgcttg aggagctacg
      421 tgctggggtc ccgggcgatg aaggcggcaa ggtcaccttg gaacaaattt ttctatccat
      481 cgtaggacag cacggcggtc agcgcggagt ggaatctcaa gtcgatgagc tgtcatggct
      541 gacctagccg gccccatcag tctgattgat caaatccgct tggtggccgg acttcgctgg
      601 cgtatggcaa tcaatagcct gcgcaaaaaa aataatattc tggatttgat cggaatggga
      661 tttgtttccc tagccgcctt gatcctgata atcggaccat gcttcgggtt ttattttgcg
      721 ggatattcat tgatctctgc cgggaggctg caatggttgg cggcgccgtt ctgggctatt
      781 tttattttct ggcaggtaat cccaattttt gccgcgggtt tcggcgcgag ctttgaattt
      841 cgaaccctgt tgcgctttcc atttagcgct agcgcatttt atctgattgg cctggcgtat
      901 ggcttggcca acttccccac gatcgcgtgt atttgttggc ttttacttat gacggcaggc
      961 gccgcggtcg caaacccgag tgttctccct gccatgttgg tcgtagttat tttggttatt
     1021 ttgttgaacg tgaccttgga acgactgatc ggctcatggc tcgaaagact tctggccaga
     1081 aggcgatcgc gtgaaatatt tttgggcgtt ttcatcctgt tgatgttttc gatgcagttc
     1141 atcatgccga tgcagcgtca atacgcaaaa agaaattcga atccagccgt ttttgtcggc
     1201 atggtcaaat accttgctcc ttttcccccg tcgctgtccg cgcgcgttct cgtgggggca
     1261 gtacgccacg atttcgtgga tatcgccgtc gggtcgtcag gattgctagg atttgtcttc
     1321 ctattttccg cattactgtg gcaaaggtac gcggcccaat atcgtggcga agaattaagt
     1381 gaatcgccgg cgcccagtcc cgcagcgtcg gtacgcgcca cccgctggcg ctcaatcccg
     1441 caaatcacca gcaccggacg aatctctccg ggattattgc caccggcgat aggccccgta
     1501 gtccgcaagg aattttctta cctggctaga aacgggtttg catttttcct gctggtgctg
     1561 cctccggcgc aaatactttt gttcggttcg cagtttggcg gtaaacatct ggcgattgcc
     1621 ggcagaggat taaatgccga cctcttcttt cccgccatga tggcctacac cgtcttggtc
     1681 acgatgggtc ctgcttacaa tgcgttcgcg tacgagggga gaggaattca aacgtacttc
     1741 accgcgccgt tgagattttc agatcttttg gcagggaaaa acctggtgtc agcggctacg
     1801 gttggatttg aagttgctct ttgcggcgtg gttttagcct gggcgtttgg tcccccctca
     1861 attccaacgc tgtttgcgac catctttgcg ctcatcttta ccgtaagcgg acaattgccg
     1921 atagccaatt gggcctcact gagttttcca cgcaagctgg aattcggttc catgcgcagc
     1981 caacgaaatt caggcgtatc ggtgtggatt atgttcggcg cgcagatcgt tcttgcggga
     2041 atttcagccc tagtgctatc cagtgctcgg tggtccggca atccgtggct gcctgcggaa
     2101 ggcttcgcat ttttggcggc agctgcgttg ggcggatact ttgcgtcgct gcaagcactg
     2161 tcggagctcg ccgagaaaaa gaaagaaatc ttgattgaag cgctttgtcg ctaggcgggc
     2221 gaggcgttcg gtcgcggatt gtgacgggtc agttccgcgt gaaggagtat tcatgaaaat
     2281 gcaatcgagt ctgctgaccg tgtt
//
LOCUS       DATCBB010000332         1193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_334577, whole genome shotgun sequence.
ACCESSION   DATCBB010000332 DATCBB010000000
VERSION     DATCBB010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334577"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..531
                     /locus_tag="VII37_03735"
     CDS             217..531
                     /locus_tag="VII37_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068865.1"
                     /translation="MEPANRKLIRPSFTEAKAEAKGQRPPPRGAGGPKRTSPPEQTNA
                     ENFYYLKQMQSKTLMSLVLRDGEVLKGVIEWYDKDCLKVNREGAPNLLVFKSNIKYMH
                     KA"
     gene            750..>1193
                     /locus_tag="VII37_03740"
     CDS             750..>1193
                     /locus_tag="VII37_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY5068866.1"
                     /translation="MFHLALSLAFIAAATTFICYRVIHVNPTTAGFAYLLCVLGIATA
                     WGSVESIVASFVAMLAFNFYFFPPVGNFTIADPQNWVALFSFLTTALVASHLSDRAKK
                     RAFEAKSRQQETEQLYALSRAILLMELSGSVGFHVAQHIAEIFDCR"
BASE COUNT          257 a          344 c          313 g          279 t
ORIGIN      
        1 aagcgcgttt ccttcgactg gctgcgcgcc gccgtcgccc gcgtcgatca actggtcctg
       61 ctggtgcgcc gcaacattca gaaatccatc gcgctcgacg cttttgccgc tgaattgcgc
      121 ggtgatgcgt agttcggcgg tggaagtttg ctcctcgctg tgttatgaca gtaactaaga
      181 agtcttaatc cccgtcaatt ccctaggagc gctcccatgg agcccgcgaa tcgcaaattg
      241 atccgtcctt cttttaccga agccaaagcc gaagccaagg ggcaacggcc gcctccccgc
      301 ggagccggtg gccccaagcg aacctcgccg ccggaacaaa ccaacgccga gaatttttac
      361 tatctaaagc agatgcagtc gaaaacgctc atgtccctgg tgcttcgcga tggcgaagtg
      421 ctcaagggcg tcatcgagtg gtacgacaaa gactgcctga aggtgaatcg cgaaggcgcc
      481 ccgaatctac tggtgttcaa atccaacatc aagtacatgc acaaggcttg agcgattagg
      541 ccgattagag ccgcggcctg cgtgacttgc agggttggat gacggcttcg attttcttta
      601 tactgtagct gcaaaatttg cattaagtgc tttgtgatca ataatataaa aagctgtaaa
      661 gaactcatca agaagtcata aagacgccgg ggctgaccct caaatcacgc taaaattggc
      721 ttcagttgcc ctcgcgtatc agcaaccgct tgtttcatct ggcgctgagc ctggcgttca
      781 tcgccgccgc caccacgttc atttgctacc gcgtgattca cgtgaaccca acgacggcgg
      841 gattcgccta cctgctatgc gtgctgggca tcgccaccgc ctggggatcg gtcgagtcca
      901 tcgtcgcgtc gttcgtggca atgttggcgt tcaactttta cttcttcccg cctgtaggca
      961 atttcaccat tgccgatccg cagaattggg tggcgttatt ttcctttctc accacggcgc
     1021 tagtggccag ccatctctcc gaccgcgcca agaaacgagc atttgaagcc aagagccgcc
     1081 aacaggagac cgaacagttg tacgccttga gccgcgcgat tctgttgatg gagctttcgg
     1141 ggtcagtcgg ttttcatgta gcccagcaca ttgccgagat tttcgattgc cgc
//
LOCUS       DATCBB010000333         1337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_10604, whole genome shotgun sequence.
ACCESSION   DATCBB010000333 DATCBB010000000
VERSION     DATCBB010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10604"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1177
                     /locus_tag="VII37_03745"
     CDS             197..1177
                     /locus_tag="VII37_03745"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY5068867.1"
                     /translation="MRIRAVRSVQAGESPEVVARSLRINRRTIYGWLAQYRRGGWGAL
                     KAKPLFGRPPKLGARAMQWVYNTVTQKNPLQLKFAFALWTRDMVAKLIKDKFGVALSA
                     NSVGRLLAQLGITCQKPLHRAQERDEALVEQWLKKEYPRIKALAQREKAEIFFGDAAH
                     MRSDHHAGRTWGKKGETPVVEATGARHGMSLISAITSRGHMRFMIKEKGGVNADVFIE
                     FLKRLITGAKRAIFLIVDRGPAHIAKKTRAFVASTNGRLRLFYLPPYSPDCNPDELVW
                     KHLKADTVGRMAITDKVDFKTKVRASMRQLQHDPKKIRSFYQKPSLKYAA"
BASE COUNT          307 a          373 c          387 g          270 t
ORIGIN      
        1 ccggagtccg aaggaaaatg tcagctatgt ttggctctcc catccaacac ccatccgtgg
       61 gataccaagc gcgctgccgt attaatcggt ccataaataa ttgctcattt ttcttgacga
      121 gcggagtctc gtgtgattcg aatgtggcat gagacaagac gatgctcgca aactggatca
      181 tgcgacgctg gaagcgatgc gtatccgcgc ggtgcgtagc gtacaggccg gcgaaagtcc
      241 cgaggttgtt gcgcgatctc tgaggatcaa ccgccgcaca atatacggct ggctggcgca
      301 gtatcggcga ggtggatggg gcgcgttgaa ggccaagcca ctttttggaa gaccgcccaa
      361 gctgggtgcg cgcgccatgc aatgggttta caacacagtg acgcagaaga atccgttgca
      421 actgaagttc gcgttcgcgc tgtggacgcg cgacatggtg gcgaagctga tcaaggacaa
      481 gtttggcgta gcgttgagtg ccaattccgt tggtcggttg ctggcgcaac tcggcatcac
      541 ctgccagaag cctttacatc gtgcgcagga gcgcgatgag gctctggtag agcaatggct
      601 caagaaggaa tacccgagga tcaaggccct tgcgcagcgc gaaaaagccg agatcttctt
      661 tggcgatgcc gcccacatgc gctcggatca tcacgcgggg cgtacttggg gcaagaaggg
      721 cgaaacgcct gtcgtcgaag ccaccggcgc acgccatggc atgagcctaa tctcggcgat
      781 cacctcgcga ggccatatgc ggttcatgat caaagaaaaa ggcggcgtca acgccgatgt
      841 cttcatcgaa tttctgaagc gcctaatcac cggtgccaag cgcgcgattt tcctgatcgt
      901 cgatcgagga cctgcccaca ttgctaagaa gaccagagct tttgtcgcga gcacgaacgg
      961 cagactgcgg ctgttttatc ttccacccta ctcacccgac tgcaatcccg atgaactggt
     1021 ctggaaacat ctcaaagccg acactgttgg ccgtatggcg atcaccgaca aggtggattt
     1081 caagaccaaa gtccgtgcat ccatgcgcca attgcaacat gacccgaaga aaatccgatc
     1141 cttctaccaa aagccatccc tcaaatacgc cgcatgaacg tgtatttact tatggactga
     1201 ttaatagccg gcccacagtc cgggcgtggc gagctccagg agatggccgt ctggatcgcg
     1261 gaaatagatg ctgtggccgc cgcgcggcca ttgggttcgg ccctcgatcg cgatgccggc
     1321 agcgcgcagc cgctctt
//
LOCUS       DATCBB010000334         1172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_334773, whole genome shotgun sequence.
ACCESSION   DATCBB010000334 DATCBB010000000
VERSION     DATCBB010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334773"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VII37_03750"
     CDS             complement(<1..923)
                     /locus_tag="VII37_03750"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY5068868.1"
                     /translation="MTAPNNERVSGNKSPQQMTCMEVWGGSQLTTRRVEMGGLDAWVY
                     SKPFGEAQRGGDVYYASSCATGRITRLLLADVSGHGNSVASTAANLRTLMRRFVNRLD
                     QTEFVRLLNQQFTALSEAGTFATAVVATFFAPTQRLIVCNAGHPRPLLYQAANREWTF
                     LGADAANDEAPNNIPLGIIDVTEYEQFDVELKSGDCVLSYTDALIESRDADGEMLGEA
                     GLLRIMRLLGDVESQLLTKTLLREITDRYPDNLTEDDVTVLLVRANGHKPRHSFREIL
                     WAQIRFAGSLIRAINPWAERPPLPDLNMANIG"
     gene            complement(934..>1172)
                     /locus_tag="VII37_03755"
     CDS             complement(934..>1172)
                     /locus_tag="VII37_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068869.1"
                     /translation="YDFKEISGAPLMARLTVDLELRETKSGSTVWTHYYSHDEPVTGK
                     DISAVVAALDRNAQRGMAEVKSSLEQYFSTRSPN"
BASE COUNT          252 a          341 c          326 g          253 t
ORIGIN      
        1 ccgatattcg ccatgttcaa atcagggagc ggtggccgtt cggcccaggg attgatcgcg
       61 cgaattaagg agccggcaaa tcggatctgc gcccaaagga tttctctgaa cgaatgacgc
      121 ggcttatgcc cgttggctcg gaccaggaga acagtgacat cgtcttcggt cagattgtcc
      181 ggatatcgat ccgtaatctc acgcagcagc gtcttggtca ggagttgcga ctccacgtcc
      241 ccgagcaggc gcatgatccg cagcagtccg gcctcgccaa gcatctcccc atccgcatcg
      301 cgggactcaa tcaacgcatc ggtgtagctg agcacgcagt cgcctgattt caattccaca
      361 tcaaactgct cgtactccgt cacgtcgatg attcctagcg gtatattatt aggcgcctca
      421 tcgttcgccg catcggcgcc cagaaaagtc cattcccggt ttgctgcctg gtacaagaga
      481 ggccgcgggt gacccgcgtt gcagacaatc aagcgctggg tgggagcgaa aaatgtggcc
      541 acgaccgccg ttgcaaaggt gcccgcctcg gaaagcgccg tgaactgctg attgagcagc
      601 cgcacaaatt ccgtttggtc caagcggttg acgaatcgac gcatgagtgt tcggagattt
      661 gcagcggttg aggcgaccga gtttccatgt ccggacacat ccgccagcaa caggcgggtg
      721 attcggcccg tggcacagct cgacgcgtaa tagacatccc cgccgcgctg cgcctcgcca
      781 aagggtttgc tgtaaaccca agcgtccagc ccacccattt caacgcgccg cgtggtgagc
      841 tggcttccac cccagacttc catgcacgtc atttgttgcg gtgatttatt tcccgagacc
      901 cgttcattgt ttggcgctgt catagttcga gtcctaattc ggagaacgag ttgaaaaata
      961 ttgctccagg cttgatttta cttcagccat gccgcgctgg gcattgcggt ccagagcggc
     1021 aacgaccgcg gaaatatcct tgccggttac aggttcgtca tgcgagtaat aatgggtcca
     1081 gaccgtcgag cccgatttgg tttcacgcaa ttccaaatca acggtaaggc gggccatgag
     1141 cggagcgccg gaaatctcct taaagtcgta ca
//
LOCUS       DATCBB010000335         4192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_205307, whole genome shotgun sequence.
ACCESSION   DATCBB010000335 DATCBB010000000
VERSION     DATCBB010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205307"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..984)
                     /gene="atpG"
                     /locus_tag="VII37_03760"
     CDS             complement(76..984)
                     /gene="atpG"
                     /locus_tag="VII37_03760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY5068870.1"
                     /translation="MATLLDFRRRIRSVKNTQQITRAMKFVAAARLRRAQEAALAARP
                     YAVELARVLRSTMARIESPEHPLLAKRPENNILAIVLTGERGLAGAFNSNVLRKAQEF
                     FRSHPGKKITTIPVGKKGRDSLRRAGFTFAAEYVNVLARVDFRTAREIANLVADLYVK
                     EAIDSVYIIFSEFKSVMVPTLAVEKLLPVEAIREDDGDKAAQPDREKKKPGEAGETPI
                     DYIYEQPEEQILAKLLPRYVETQILRAMLESSAAEHAARMTAMEAATNNANDVIEALT
                     LHMNKVRQAAITKEIIEIVSGASYGA"
     gene            complement(1011..2660)
                     /gene="atpA"
                     /locus_tag="VII37_03765"
     CDS             complement(1011..2660)
                     /gene="atpA"
                     /locus_tag="VII37_03765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY5068871.1"
                     /translation="MTKINADEISKILREQIENYEQTVSVDEVGSVISVGDGIARVHG
                     LEKVMAGEMLAFPKNIFGIALNLEEEEVGVVLLGESSDLQEGDVVKRTNTIMSVPVGE
                     AMVGRVVNPIGEPLDGKGPIESKQRNPLERIAPGVLDRQPVREPLQTGIKAIDAMIPI
                     GRGQRELIIGDRQTGKTAVILDTIINQKGGDVICIYCGIGQKRSTIAQVVKTLTDAGA
                     MDYTIVVASTASEPASLQYIAPYAACAMGEFFRDTKRHAVTFYDDLSKHAQAYREISL
                     LLRRPPGREAFPGDVFYLHSRLLERAAKLNDKLGAGSLTSLPVIETQAGDVSAYIPTN
                     VISITDGQIYLEADLFNAGIRPAINVGISVSRVGGNAQIKAMRQVAGSLRLDMAQYRD
                     LAAFAQFGADQLDKATQAQLGRGQRLTEILKQDQYAPFTVEKQVLSLFVATSGALDSI
                     PVPSVRRFEREFLAFVETNNSGILKSIAEKKALDDNIKGEIKKAVDAFKERFATDVEE
                     GGESAAKAAAAPAKNQDGTGAVVTPKSEDPTAKKEATPVAK"
     gene            complement(2794..3315)
                     /gene="atpH"
                     /locus_tag="VII37_03770"
     CDS             complement(2794..3315)
                     /gene="atpH"
                     /locus_tag="VII37_03770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY5068872.1"
                     /translation="MQYANAFADIAIAQGAPQTSVRQLADFGEAFAESGELRNFLGSP
                     AVDTRVKHRVIEKIVAQQGAGRIVRNFLFVITDHHRAHMLPEIIAAVQDVVRQRQGIA
                     EAEVSSAVELSAAQKTELARTLSRLTGKKIEPKYSLDPAILGGAIARVGDTIYDGSLR
                     SRLAEMRTRLSQE"
     gene            complement(3327..3923)
                     /locus_tag="VII37_03775"
     CDS             complement(3327..3923)
                     /locus_tag="VII37_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY5068873.1"
                     /translation="MNLPVKTAKRYAQIAASLLFSLLFLALNAHAEEAGSASQQSAEN
                     LFKWINFALVAGVIVWLCLKKGPAFFGRRAEVISSEIQKSTEAKKQAEQQLQDAETKL
                     QNLEKEVADLRATAQRESVAEAERIRNLTVTDAKKIDDAGKAEVHAAERAARLQLKAF
                     AANLAINGAESLLLKQLTPAAQEALINNFVKTLDGRPN"
     gene            complement(3920..>4192)
                     /locus_tag="VII37_03780"
     CDS             complement(3920..>4192)
                     /locus_tag="VII37_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068874.1"
                     /translation="HQDALKQARTEIYVEQEAARKRVLDERAAQLKEARAKTATVISA
                     AKERIVSEVLAARREIEASITPFAALVARRVLQSTRPPAAPPRESR"
BASE COUNT          830 a         1275 c         1098 g          989 t
ORIGIN      
        1 attggatgtc cagcactgga ccaatcacct gaacgactcg tcccatgcgt ccgcgttttt
       61 ccgtcatgct ttctcctagg cgccgtacga ggcgccgctg acaatttcga taatttcctt
      121 ggtgatagcc gcctggcgca ctttgttcat gtgtagcgta agcgcttcga tcacatcatt
      181 ggcgttgttc gtggcagctt ccatggccgt catgcgcgcg gcgtgttcag ccgcggaaga
      241 ttcgagcatg gcgcgcagaa tttgtgtttc aacgtagcgc ggaagcagct tcgccagaat
      301 ttgctcttca ggctgctcgt aaatgtagtc gatgggagtt tcgccagcct cgccgggctt
      361 cttcttttcc cgatcaggct gagcagcttt atcgccgtca tcctcgcgaa tagcttccac
      421 aggcagcaac ttttccactg ccagagtggg aaccatcacg cttttgaatt cactgaaaat
      481 aatgtaaacc gaatcgatcg cctccttgac gtacagatcg gcgacaagat tggcaatctc
      541 tcgcgccgtt cgaaagtcca cgcgtgccag gacgtttaca tattccgcgg caaacgtgaa
      601 gcccgctctg cgcagcgagt cgcggccctt ttttcccact ggaatcgttg taattttctt
      661 gcccggatga ctgcgaaaaa attcctgcgc cttgcgcagc acgttgctgt tgaacgcccc
      721 ggccagtccg cgttcgccag taagcacgat ggctagaata ttgttttccg gccgcttggc
      781 gagcaacgga tgctccggcg attcgatgcg cgccatggtg gaacgcaaca ctcgtgcgag
      841 ttcgaccgcg taaggccgag cggccagcgc ggcttcctgt gcgcgacgca gtcgtgccgc
      901 cgcgacaaac ttcatcgcgc gagtaatctg ctgggtgttt ttcaccgagc ggatgcggcg
      961 ccggaaatcg agtaatgtag ccatgtttta aaaattatgc gctgagcaga ttacttcgct
     1021 accggcgttg cctctttttt cgccgttgga tcctcggact tgggcgtcac caccgctccc
     1081 gtgccatcct ggttcttcgc cggggcagcc gcagccttgg ctgccgattc gccgccttct
     1141 tctacatccg tggcaaagcg ttccttgaac gcgtccacag cctttttgat ttcgcccttg
     1201 atgttatcgt ccagcgcttt cttttccgcg atggacttga gaatgccaga attgttggtc
     1261 tccacaaacg caagaaactc acgttcgaag cggcgcactg agggaaccgg aatgctgtcc
     1321 agcgctccgc tggtggccac aaataaactg agcacctgct tctcaaccgt aaacggagcg
     1381 tattggtcct gcttcaaaat ttccgtcagg cgctggcctc gacccagttg agcttgcgtg
     1441 gctttatcca actggtccgc gccaaattgc gcgaacgccg ccaagtcgcg atattgcgcc
     1501 atgtccaaac gaagcgaacc ggccacctgc cgcatcgcct tgatctgcgc gttgcccccc
     1561 acgcggctaa cagaaattcc cacgttaata gccggtcgga tgcccgcgtt gaaaagatcc
     1621 gcttccagat agatttgccc gtcggtaatg gagatgacgt tggtcggaat gtacgcggaa
     1681 acgtctccgg cctgcgtttc gattaccggc aaagaagtca gcgaacccgc gccaagttta
     1741 tcgttgagct tggcggcccg ctccagcaat cgagaatgta ggtaaaacac gtcgccaggg
     1801 aaagcttcgc ggcccggcgg acgtcgcaac agcaaagaaa tttcgcgata ggcctgcgcg
     1861 tgtttcgaaa gatcatcgta gaaagttacc gcgtgtcgct tggtgtcgcg gaaaaactcc
     1921 cccattgcgc aagccgcata cggcgcgatg tattgcagcg aagcaggctc cgatgcggtg
     1981 gaggccacca cgatggtgta gtccatcgca cccgcgtccg taagcgtctt caccacttgc
     2041 gcgatcgtcg agcgcttttg cccaatgccg cagtaaatac aaattacgtc gccacctttt
     2101 tgattgatga tggtgtccag aatcaccgcg gtcttgccgg tctggcgatc gccgatgatc
     2161 aactcgcgct ggccgcggcc gatcggaatc atcgcgtcga tcgctttgat accggtctgc
     2221 agtggctcac ggaccggctg ccggtccagc acgcctggcg cgatgcgctc cagcggattg
     2281 cgctgtttcg attcgatcgg acccttaccg tcgagcggtt cgccaatcgg attaaccact
     2341 cgcccaacca tagcttcgcc tacaggcacg gacataatcg tattcgtgcg cttgacgaca
     2401 tcgccttcct gcaaatcgct cgactcgccc agcaacacca cgccgacttc ttcttcctca
     2461 agattcagcg caataccaaa aatgtttttt gggaaagcga gcatttcccc ggccatcact
     2521 ttttccagac catgcacgcg cgcaatcccg tcgcctacgc taattaccga accgacttca
     2581 tcgacggaaa cggtctgctc atagttttcg atctgctcgc gcagaatctt gcttatttca
     2641 tcggcgttaa ttttggtcat tcttgctcct aatctcgttt gaatcggtta gattcaagtc
     2701 ttcgctttag gttcctgata cttagtttta gtgcgaagcc aactgcgtgc tacctaacta
     2761 ccccccaaaa gaccggcgcc aagccagtca caatcattct tgcgaaagcc gcgtgcgcat
     2821 ttccgccagg cggctacgta aagaaccgtc atagatagta tcgccaaccc gcgctatggc
     2881 accgccaaga atcgccgggt cgagagaata tttcggctcg atctttttac ccgtcaagcg
     2941 gctcagtgtt ctagctagct ccgttttctg cgcggcggac agctccacag cggaagaaac
     3001 ttccgcctcc gctatccctt ggcgctgcct caccacgtcc tgcacagcgg caatgatctc
     3061 cggtagcatg tgcgcgcgat gatggtcggt aatcacaaaa agaaaattgc gaacaatcct
     3121 acctgcgccc tgctgcgcga cgattttttc gatcacccgg tgcttgacac gcgtgtcgac
     3181 cgctggacta cccaaaaaat tacgcaactc cccggactcc gcaaaggcct cgccaaaatc
     3241 tgccaactgc ctcaccgacg tttgcggcgc gccttgagcg atcgcgatat ccgcgaacgc
     3301 gttcgcgtac tgcatgctcg ctgacttcaa ttgggccgcc cgtccagagt cttgacgaaa
     3361 ttattgatga gcgcttcctg ggccgcgggg gtgagttgct tcagcagcag cgattcggcg
     3421 ccgtttatgg cgaggttagc cgcgaaagcc ttcagttgaa gccgcgcggc acgctccgca
     3481 gcgtgcactt ccgcttttcc ggcatcgtcg attttttttg cgtccgtgac cgtgagattc
     3541 cggatgcgct cggcttccgc aacggattcg cgttgcgcgg tagcacgtaa atcggccact
     3601 tctttctcca gattctgcaa cttggtttcc gcatcctgca gctgctgctc cgcctgcttt
     3661 ttcgcttcgg tgctcttctg gatctccgag ctgatgactt ccgctcgccg gccgaaaaag
     3721 gccgggcctt tcttaaggca tagccagacg atcaccccgg caaccagcgc gaagttaatc
     3781 catttaaaaa gattttcagc ggattgttgc gacgcggagc cagcctcttc cgcgtgcgcg
     3841 ttcagcgcaa ggaaaagcag cgagaacaac agggacgccg caatttgggc gtaccgtttc
     3901 gcggttttca ctggcaggtt catcgcgact ccctcggagg agcagccggc ggccgtgtcg
     3961 attgcaacac gcgccgggct accaaggcgg caaatggagt aatgctcgct tcaatttcac
     4021 ggcgcgcggc aagaacctcg gacacgatcc gctccttagc tgcactaatc accgtggccg
     4081 tcttcgcccg cgcttctttc agttgcgcgg ctcgctcgtc gagaactctc ttgcgagcgg
     4141 cttcctgttc tacataaatt tccgtgcgag cttgcttcag cgcgtcttga tg
//
LOCUS       DATCBB010000336         1329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_270420, whole genome shotgun sequence.
ACCESSION   DATCBB010000336 DATCBB010000000
VERSION     DATCBB010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270420"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1329
                     /locus_tag="VII37_03785"
     CDS             <1..>1329
                     /locus_tag="VII37_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5068875.1"
                     /translation="YSDLDILFLFGNEKTEAEYRPLISEFSRVLWDIGFRVSSAGRTI
                     DECKRIEEDNAEFHLALLDRRFLAGDKTLFERLDTKVLPGSEKQARPFLLAQLQKLTK
                     DRLTRYGNTIFHLEPNVKESPGGLRDYQATVWMQQISGEKKDARPTMTAEDELVRKAV
                     DFMGEIRCFLHYSNGRNDNTLTYELQEAAAARYLGIPRVFSSTPARQKDVEAERGTSE
                     HDAAEWMRMYFRQARTLNRRLLRHMEQKTQLTTLSLRERIFSAARVPKLETSSVRPFM
                     ERDGLLEIVAPGALSDRAITFSLFLAAASTGVPLSREAERTIAYIQTHPEVPAQNTQI
                     SWATLREILSADYPGVALRPMQRLGLLTEVLPEFAHIDSLVVRDYYHRYTVDEHSLRT
                     IEHLQELGDGSDALGKNFAPLWKTLERRDLLILAMLLHDVGKGMAVENHVT"
BASE COUNT          309 a          297 c          433 g          290 t
ORIGIN      
        1 tactccgatc tggatatttt atttttattt ggcaacgaaa aaacggaggc ggaataccgg
       61 ccgttgatca gcgaattttc ccgggtgctg tgggacattg gttttcgggt gagctccgcg
      121 gggcgaacta tcgatgaatg caagcgcata gaagaggata acgcagagtt tcacctggct
      181 ttgctggacc ggcggttcct ggcgggggat aaaacgcttt ttgagcggct ggatacgaag
      241 gtgctgcccg gttcggaaaa gcaggcgcgg ccgtttttgc tggcacagct tcaaaaattg
      301 acgaaagaca ggctcacacg ttacggcaat acgatttttc atcttgagcc taacgtgaag
      361 gaatcgccgg gcgggttgcg cgattatcag gcgacggtgt ggatgcaaca gatcagcggg
      421 gagaaaaaag atgcacggcc gacgatgacc gcggaagacg agctggtgcg aaaagcggtg
      481 gattttatgg gcgagattcg ctgttttttg cattacagca atgggcggaa cgacaacacg
      541 ctgacgtatg aactgcaaga agcggcggcg gcgcgttatc tgggaattcc tcgagtattt
      601 tcgagcactc cggccaggca gaaagatgtt gaagctgagc gaggcacttc ggaacatgac
      661 gcagcggaat ggatgcgcat gtatttccgg caagcgcgga cattgaaccg gcgattgctg
      721 cggcacatgg aacagaaaac gcaactcacc acattgtcgt tgcgggagcg catttttagc
      781 gcggcgcgcg tgcctaaatt ggaaacgagt tcggtaaggc cttttatgga gcgcgatgga
      841 ttacttgaaa ttgttgcgcc tggagcgcta tcggatcgag cgattacgtt ttcgcttttt
      901 ttagctgccg ccagcacagg cgtgccgttg agccgggagg cggaacgcac catcgcgtat
      961 atccaaacgc atccggaggt gccggcgcag aatacgcaga tatcgtgggc cacgctgcga
     1021 gaaattctga gcgcggatta tcctggtgtg gcgctgcggc cgatgcagag attgggattg
     1081 ttgacggaag ttttgccgga attcgcgcac atcgattcgc tggtggtgcg cgattactac
     1141 catcgctaca cggtggatga gcattcgctg cgaacgattg agcatttgca ggaattaggg
     1201 gacgggagcg acgcgctggg gaaaaacttt gcgccgctat ggaaaacgct ggagcggcga
     1261 gatctgctga tcttggcgat gctgctgcat gacgtgggca agggtatggc ggtggaaaac
     1321 cacgtgaca
//
LOCUS       DATCBB010000337         1108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_334838, whole genome shotgun sequence.
ACCESSION   DATCBB010000337 DATCBB010000000
VERSION     DATCBB010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334838"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VII37_03790"
     CDS             <1..1008
                     /locus_tag="VII37_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY5068876.1"
                     /translation="RYQTAHVTHANLNEHGAIASVSTREGVTLSADLFIDCSGFAGLL
                     ISQGLKTPYVSFSENLFNDAAVAMPTSIDKTIPSETVSTAMKHGWAWKIPLTERFGNG
                     YVYSSQFCSADQAESELRERLGLLESDTPARHLKMKIGRVTKHWNKNCLAVGLSQGFI
                     EPLEATALLFIQQTAAVFVDFLEQGDLSEAAQARFNTHVNEHFEGTRDYIVTHYKTNS
                     RTDTEYWRANAANLNLSEPLKQLLNLWMSGNGIAPEVRKQSLGRGYPVFSWYSIMSGM
                     GIFPDPGNLRPPTAEEKTFGIAEIDNLLERSAANYKDHREVLSNIPPRRSAQTLQVYF
                     W"
BASE COUNT          270 a          301 c          302 g          235 t
ORIGIN      
        1 cgttatcaga ccgcgcacgt gactcacgca aatttgaatg aacatggcgc catcgcgtca
       61 gtaagcacgc gggagggcgt cacgctcagt gctgatttgt tcatcgattg cagcggattt
      121 gcggggctgc tcataagcca aggattgaaa acgccttatg tgagcttctc ggaaaatcta
      181 ttcaatgacg ccgccgttgc catgccaacc tccatcgaca aaacaattcc ctctgagacg
      241 gtctccacgg ccatgaagca tggatgggca tggaagattc cgctcaccga gcgcttcggc
      301 aacggctatg tttacagttc gcagttttgc tccgccgatc aggccgaatc ggaactgcgc
      361 gaacgcttag ggctgctcga atcggacaca cccgcgcgcc acctaaaaat gaaaataggg
      421 cgggttacca agcactggaa taagaactgt ctagcggtcg gactgtcgca agggtttatt
      481 gaacccttgg aggcgaccgc gttgctgttc attcaacaaa ccgcggctgt gttcgtcgat
      541 tttctcgagc aaggagactt gagcgaagcg gcacaagcgc gcttcaacac gcacgtcaac
      601 gaacatttcg aaggcactcg cgactacatt gtgactcact acaagaccaa cagccgcacc
      661 gataccgaat attggcgggc gaatgcggca aacttaaatt tatcggaacc gctaaagcaa
      721 ctgctgaatt tgtggatgtc aggcaatggc atcgcgcctg aggtgcgcaa gcagtcgttg
      781 gggcgcggct atcctgtatt ctcctggtac tcgatcatgt caggcatggg cattttcccg
      841 gatcctggca atttgcgccc acctacagct gaggaaaaaa ccttcggaat cgcggaaatc
      901 gacaatttgc tcgagcgcag cgcagccaac tataaagatc atcgcgaagt tctgagcaac
      961 attccgccgc gccgcagcgc tcagacactg caggtttact tttggtgaag gagcattcgg
     1021 gactgaccat cgagcagatt cggcgcgacg tgctgccgaa taagcatcct gcgatattgc
     1081 gcggattggt gcgcaactgg cgcgcggt
//
LOCUS       DATCBB010000338         1293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_465094, whole genome shotgun sequence.
ACCESSION   DATCBB010000338 DATCBB010000000
VERSION     DATCBB010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465094"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VII37_03795"
     CDS             complement(<1..1283)
                     /locus_tag="VII37_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5068877.1"
                     /translation="MIAIVIAAFLFQPFREWTQSRLDRFFYRDRLDYRRTLIEFGRTL
                     TNEVRLEPMLGSVMDRVSQTLLVDRLAIFQEDPTHPGHMRLARSMGVRLTEPLDLGFL
                     EPARPEFARGALFFESPRAARDVSDTVRRTLEQLDLNYFVPCRIREHTVAVLGLGKTV
                     DGDFLSSDDVELVETIAGYVAIALDNSQLYTSLEQKAIEIVRLKDFSENIVESLNVGV
                     LAVDLEGLVESWNTRMESLFGVARQQAVGRPLNSLLPEELAREITARGDEELITGIYK
                     QRLQHLGKTLTLNVSITPLVSKSGERIGRLLLFDDVTQRERMEEQMTQTEKLTSLGLL
                     AAGVAHEVNTPLAVISNYIQMLAKQMPDGDPKQAIIEKIVKQTFRASEIVNNLLNFSR
                     TGAAEVADIDVNRVVEETLSLVAHPLKTSQIQVVR"
BASE COUNT          270 a          439 c          310 g          274 t
ORIGIN      
        1 tgccgcacca cttgtatttg cgaagtcttc aacgggtgcg ccaccagcga tagcgtctct
       61 tcgactacgc ggttcacatc gatatccgct acttccgccg cgcccgtgcg cgaaaaattc
      121 agcagattgt tcacaatctc gctggcgcga aacgtctgtt tcacgatctt ttcaatgatc
      181 gcctgtttcg gatccccatc cggcatctgc ttcgccagca tctggatgta attcgaaatt
      241 accgccagcg gagtattcac ttcatgcgcc acgcccgccg ccagcaagcc cagtgacgtc
      301 agcttttccg tctgcgtcat ctgttcttcc atccgctccc gctgggtcac atcgtcaaac
      361 aacagcagcc gcccgatgcg ttctcccgac ttgctcacca gcggcgtaat cgaaacattc
      421 agcgtcagcg tcttccccaa gtgctgcagt cgctgcttgt aaattcccgt aatgagctct
      481 tcatcgccgc gcgcggttat ttctctggcc agctcttccg gcagcaggga attcagcggc
      541 cgccccaccg cctgttgccg cgccactcca aacaggcttt ccatgcgcgt attccaggat
      601 tccaccagcc cttccagatc caccgccagc acgcccacgt tcagcgactc cacgatgttt
      661 tcgctgaaat ccttcagccg cacaatttcg atggccttct gttccagcga cgtgtaaagc
      721 tgcgaattat ccagcgcgat cgccacgtaa cccgcaatcg tctccaccag ctccacatcg
      781 tcgctcgaaa gaaaatctcc atctacggtt ttacccaggc cgagcaccgc cacggtatgt
      841 tcgcgaatcc ggcagggcac aaaataattc agatccagtt gctccagcgt tcgccgcacg
      901 gtgtcgctga catctctcgc cgcgcggggc gactcaaaaa agagcgcccc acgggcgaac
      961 tcgggccggg ccggctcgag gaagccaaga tcaagtggtt cagtcaatcg cacgcccatc
     1021 gaacgtgcca gccgcatgtg gccggggtgc gtcgggtctt cctggaaaat cgccaaacga
     1081 tccaccagca gtgtttgcga tacgcgatcc attaccgagc ccagcatcgg ctccaaccgc
     1141 acttcgtttg tcagcgtgcg gccgaactca attagcgtgc gccggtagtc aaggcggtcg
     1201 cgataaaaaa atctatccag cctggattgc gtccattccc ggaacggctg aaacagaaac
     1261 gccgcgatca cgatagcgat catcccgcca acc
//
LOCUS       DATCBB010000339         1090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_237167, whole genome shotgun sequence.
ACCESSION   DATCBB010000339 DATCBB010000000
VERSION     DATCBB010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237167"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VII37_03800"
     CDS             complement(<1..743)
                     /locus_tag="VII37_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5068878.1"
                     /translation="MSEKSKLQTPPAPEDQTTSPRRPSAAEKAWEENTLKPTLEKSPE
                     RRKEFTTISAHPIRRLYTEADLPNWTPEKDLGLPGEPPYTRGIHSTMHRGRLWTMRQF
                     AGFGTAEDTNARFCYLLAQGQTGLSTAFDLPTLMGYDSDHALSEGEVGKCGVAISSLA
                     DMEVLFDKIPLASVTTSMTINSPAAIIWAMYLAVAEQQGADWKKLSGTLQNDILKEYI
                     AQKEYIYPPEPSMRVVIDTFEFGVKNTPK"
BASE COUNT          213 a          239 c          375 g          263 t
ORIGIN      
        1 aatttcggtg tgttttttac gccgaactca aaagtgtcga tgaccacgcg catcgaaggt
       61 tcgggcggat agatatattc cttttgcgcg atgtattcct tgaggatgtc gttctgtagc
      121 gtgccggaga gttttttcca gtcggcgccc tgctgctcgg cgacggccag gtacatggcc
      181 cagatgatgg cggcgggcga attgatggtc atggaggtgg tgacgctggc cagcggaatt
      241 ttgtcgaaga gcacttccat gtcggcgagg gaactgatgg cgacgccgca tttgccgact
      301 tcgccttcgg agagggcgtg gtcggagtcg tagcccatca atgtggggag atcgaaggcg
      361 gtggagaggc cggtctggcc ttgtgcgagt aggtagcaga aacgcgcgtt ggtgtcttcg
      421 gcggtgccga agcccgcgaa ctggcgcatg gtccacaagc ggccgcggtg catggtggag
      481 tgaatgccgc gggtgtaggg cggttcgccg gggaggccta aatctttttc gggagtccag
      541 ttggggagat ccgcttcggt gtagaggcgg cggatgggat gagccgaaat agtcgtgaat
      601 tccttgcggc gttccggact tttctccagc gtgggcttaa gagtattttc ttcccaggct
      661 ttttcggcgg cggaagggcg gcgcggggaa gtcgtttgat cttccggagc gggtggagtt
      721 tggagtttcg atttttccga catggagagc ggctcccgag atcacgcatt aaaaaatcga
      781 ggtaaacgat gcgtagatta atgctatgcg aaacacgaga gacgggcaag ggaagggatg
      841 ctgggaagag gccaccgttt cctgtgcgct aatcttgagg ttttgcagcg gtgcaagagt
      901 agcgagaaat gcgggcggct tcgtaagttg tgcaaattat ttaatgcagc gccgttggcc
      961 gctatgcgcg aacagcggtt tatttctcga gattcacatt ccaattgcga atgatggtga
     1021 gcggtggcac ctcgttctgg tccatcaggg tgttgaacag aaacttagtt ccattttgcg
     1081 ccaccgcgta
//
LOCUS       DATCBB010000340         2669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_43697, whole genome shotgun sequence.
ACCESSION   DATCBB010000340 DATCBB010000000
VERSION     DATCBB010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43697"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2403
                     /gene="secA"
                     /locus_tag="VII37_03805"
     CDS             <1..2403
                     /gene="secA"
                     /locus_tag="VII37_03805"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY5068879.1"
                     /translation="NEFGFDYLRDNMKYDLANCVQRGHHFAIVDEVDSILIDEARTPL
                     IISGPSEESTDKYAKIDKIIPKLIQDIDYTIDEKHRTATLTEEGVSKTERLLGLGNLY
                     DPAHMESIHHVYQALRAHTLYKNDVDYVIKNGEVIIVDEFTGRQMPGRRWSDGLHQAV
                     EAKEGVKIERENQTLATITFQNYFRMYKKLAGMTGTAETEAAEFSKIYNLDVVVIPTN
                     RNLIRKEHPDIVYRTEKEKFTAVVNGIMQEDDSFANGIRHYHERGQPVLVGTISIEKS
                     ETIAALLKAAKIPHEVLNAKQHERESRIVAQAGRKGAVTVATNMAGRGTDILLGGNPE
                     QMTRDYFLKNKMAMPYAAAPAVIAATDSSGDGAAAPVTPMVLFQHEGKIFQVPADQWK
                     PVYNQFAEQCKAEHDEVVALGGLHILGTERHEARRIDNQLRGRAGRQGDPGSSRFFLS
                     LEDDLMRIFGGERVKALMFRLGMTEGVPIESGLISRRIENAQKSVEAQNFDARKHLLE
                     YDDVMNKQRETIYGIRRSALEGKDQRDYVLGIAEDVARELVNTFCPREQHPDQWNATQ
                     FLAEMNAQFGIDAKAAGADPGTLTHDALSDAAAEAVNARYAEKETQFGADLMRWLERR
                     IILDVVDSQWKDHLLSLDHLKEGIGLRGYGQKDPLVEFKKEAFTLFEDMMARIDNETV
                     RYLFHIQVQQGEPPQQLEQRPEPPRPPRQDARSAMASAAARASLGDSDSQNSTPQHLP
                     SVAREIERKQARLQRDLQYQTGPAQAEAPKPVRAAAKVGRNDPCPCGSGKKYKKCHGA
                     NV"
BASE COUNT          661 a          760 c          747 g          501 t
ORIGIN      
        1 aacgaatttg gcttcgacta tctgcgcgat aacatgaaat atgacctcgc caattgtgtt
       61 cagcgcggcc atcacttcgc catcgtcgat gaagtggact ccatcctgat cgacgaagcg
      121 cgcaccccct tgattatttc cgggccttca gaagagtcca ccgacaaata cgccaagatc
      181 gacaagatta ttcccaagtt gatccaggac attgattaca ccatcgatga aaaacatcgc
      241 accgccacgc tgaccgaaga aggcgtcagc aagaccgaac gcctgctcgg tctgggcaat
      301 ttgtacgacc ctgcgcacat ggaatcgatt caccatgtct atcaggcgct ccgcgcgcac
      361 acgctgtaca agaatgatgt ggattacgtc atcaaaaacg gcgaggtcat catcgtcgac
      421 gagtttaccg ggcgccagat gcccggccgc cgctggtccg acggattgca ccaggctgtg
      481 gaagccaaag aaggcgtgaa gatcgagcgc gagaatcaga ccctcgccac tattaccttt
      541 cagaattatt tccgcatgta caagaaactg gccggaatga ccggtacggc ggaaacggaa
      601 gctgcggaat tcagcaagat ttataacctc gacgtcgtgg tcattcccac caaccgcaat
      661 ttaattcgca aagagcatcc cgacatcgtt taccgcacgg aaaaagaaaa attcaccgcg
      721 gtggtaaacg gcatcatgca ggaagacgat tccttcgcga acggcattcg ccattatcac
      781 gaacgcgggc agccggtatt ggtcggcacg atctcgatcg aaaaatcgga aacgatcgcc
      841 gctttgttaa aggccgcaaa aatccctcac gaggttttga atgccaagca gcacgagcgc
      901 gaatctcgca tcgtggcgca ggctggacgc aagggcgctg tcaccgtggc cactaacatg
      961 gccggccgcg gcacggacat tctactcggc ggaaatcccg agcagatgac gcgcgattat
     1021 ttcctgaaaa acaaaatggc gatgccgtat gccgcggctc ccgcggtgat tgccgctact
     1081 gacagctccg gcgatggagc ggcggcaccg gtgacgccga tggtgctgtt ccagcacgaa
     1141 ggcaaaattt tccaggttcc ggccgatcag tggaagcctg tttataatca gttcgccgag
     1201 cagtgcaaag cggagcacga cgaagtcgta gctctcggcg gcttgcacat tcttggcacc
     1261 gaacgtcacg aagcccgtcg tatcgacaat caattgcgcg gccgagcggg acgccagggc
     1321 gaccccgggt cttcgcgctt tttcctctcg ctagaagacg acttgatgcg cattttcggc
     1381 ggtgaacgcg tcaaggcgct gatgttccgc ttgggtatga ccgaaggtgt gcccatcgaa
     1441 tcaggattga tctcgcggcg catcgaaaat gcccagaaat ccgtcgaagc gcaaaacttt
     1501 gacgcccgca aacacttgct ggaatacgac gacgtcatga acaagcagcg cgaaaccatt
     1561 tatggtattc gccgctctgc gctcgaaggc aaagatcagc gcgattatgt tctgggcatc
     1621 gcggaagatg tagcgcgcga gctggttaac acgttttgcc cgcgcgagca gcatcccgat
     1681 caatggaatg ccacgcagtt tttagcggaa atgaatgccc agtttggcat cgacgcgaaa
     1741 gctgctggcg ccgaccccgg cacgctgacc cacgacgcgc tttccgatgc cgctgctgaa
     1801 gcggtgaacg cgcgctacgc ggaaaaggaa acgcagtttg gcgcggacct catgcgctgg
     1861 ctggagcgcc gcatcattct cgacgtagtg gactcgcaat ggaaggatca ccttctttcg
     1921 ctcgatcatt tgaaagaggg catcgggctc cgcggatacg ggcaaaaaga tccgttggtc
     1981 gagtttaaaa aggaagcctt cacgctcttc gaagacatga tggcgcgcat cgacaatgaa
     2041 acggtgcgct atttatttca catccaggtg cagcagggag agccgccgca gcaactcgaa
     2101 cagcgtcccg aaccgcctcg gccgccgagg caggatgcgc ggagcgccat ggctagcgcc
     2161 gcagcccgag cctctttggg cgactccgat tcgcaaaatt cgacgccgca acatttgcct
     2221 tccgtggcgc gcgaaatcga acgcaagcag gccaggctgc agcgcgacct gcagtatcag
     2281 accggtccgg cgcaagctga agctcccaag cccgttcgcg cggccgcgaa agtcgggcgc
     2341 aacgatccgt gtccctgcgg atcaggcaaa aaatacaaga agtgccacgg cgcgaatgtc
     2401 tgatcgcgaa acgcggctca tgcaattatc gtgaaagtga attatccgaa ctgaagtagt
     2461 ttcgtaccag cgcgagagca atccaaagga aataaaatgc cgccaggaac aggtcccatg
     2521 cagcagcccg tacgtcagcc aattcctggc gtaaaaaatc tcgttgcggt tgccagcgga
     2581 aaaggtggcg tagggaaaac caccgtcgcg gtaaatcttg ctttggcgct cgcgaagctt
     2641 ggccacaaag tcggattgat ggacgccga
//
LOCUS       DATCBB010000341         1077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_75182, whole genome shotgun sequence.
ACCESSION   DATCBB010000341 DATCBB010000000
VERSION     DATCBB010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75182"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VII37_03810"
     CDS             complement(<1..794)
                     /locus_tag="VII37_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HEY5068880.1"
                     /translation="MAHNYDALILGAGAAGLMCAIEAGKRGRRVAVLERAARAGKKIL
                     ISGGGRCNFTNLHCRPEKFISANPHFAKSALARYTPSEFIALVESHSIPYHEKTLGQL
                     FCDRSARDITDMLEAECRAAKVDVFLNTTIQEVRHAAEFTVHAAEAEFSAPAIVVATG
                     GLTIPKMGATDFGYQIARQFGIKIREPRPALTPLLLQSRDRSTYCDLAGVSTEVIAST
                     DHHAFREAMLITHQGLSGPAILQISSYWNKGEPLHIDLAPHREVFAS"
BASE COUNT          240 a          257 c          340 g          240 t
ORIGIN      
        1 gaggcaaaga cctcgcgatg cggagcgaga tcgatgtgca acggttcgcc tttgttccag
       61 taggaggaaa tttgcagaat ggcggggccg cttaggcctt gatgagtgat gagcatggct
      121 tcacgaaaag cgtggtgatc ggtagaggcg atcacttcgg tggagactcc ggctaaatcg
      181 caatatgtgg atcgatcacg gctttgcaat agaagtggcg tgagagctgg gcggggttcg
      241 cgaatcttta ttccgaactg acgggcaatt tgatagccaa aatccgtggc gcccattttg
      301 gggattgtta atccgccggt ggcaacgacg atggccggag ctgaaaattc tgcttcagcg
      361 gcgtggactg taaattctgc ggcgtggcga acttcctgaa ttgtggtgtt gaggaaaacg
      421 tcgactttgg cggcgcggca ctccgcttca agcatgtcag tgatgtcgcg cgccgaacgg
      481 tcgcagaaaa gctggccgag cgttttttcg tggtacggaa tcgaatggct ttcgaccagc
      541 gcgatgaact ccgacggtgt gtagcgcgcg agtgccgatt tggcgaagtg tgggttggcg
      601 gagataaact tttctgggcg gcaatgaaga ttggtgaaat tgcagcgccc tccgccggag
      661 ataaggattt ttttgccggc gcgcgcggcg cgttcaagga cggcaacgcg tcgcccacgc
      721 ttccctgctt cgatggcgca catcaggcca gcggcaccag caccgagaat tagggcatca
      781 tagttgtgag ccatagtcga tttttgcgaa atgccagcca gcgctgatcg tgcgctcgca
      841 ccatatcagg gtaaaatcgt agcaggataa actaactaca ggagcagacc ccaggaattt
      901 gccaccgatt atcagtgcac agggattatc caagaaatac ggcagcacgc cattgtttcg
      961 agacatttcg tttacggttt cgagcggcga ttgcattgga gtcattgggc ccaatggctc
     1021 cggcaagtcc accttgttgg agattctggc cggccgggtg acacctgaca ccggaga
//
LOCUS       DATCBB010000342         1398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_237238, whole genome shotgun sequence.
ACCESSION   DATCBB010000342 DATCBB010000000
VERSION     DATCBB010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237238"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1398
                     /locus_tag="VII37_03815"
     CDS             <1..>1398
                     /locus_tag="VII37_03815"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEY5068881.1"
                     /translation="EGKWLAWLGITAEQLAHISALQEIAADIRQAGFKHALLLGMGGS
                     RLCPEVLRMTFGKIDGYPELHVLDSTDPAQIKAIESKVDLSSTVCIVSSKSGSTLEPN
                     IYKQYFFERIKEKVGEGEVGKRFIAITDPGSKMQEVAERDKFRKIFMGVPGIGGRYSA
                     LSNFGMVPAAIMGIDVAKFLNNTAEMLKACGAAVPADSNPGALLGMVLGVAANHGRDK
                     VTIVASPGISDLGAWLEQLLAESTGKVGKGIIPVDREPLASPDSYGDDRVFAYLRLET
                     APDKSQDTAIQALEAAGHPVVRIAVDNIYNLGQEFFRWEIATAVAGSIIGINAFNQPD
                     VEASKIETKKLTSEYEKSGKLPDESPFFDGDGIKLYADEKNIEVLKGKPRLAEVLKAH
                     LGRLRAGDYFAVLGYITMNDANEKAMQGIRLAVRDRKKVATVLGFGPRFLHSTGQAYK
                     GGPNSGVFLQITCDDA"
BASE COUNT          331 a          340 c          432 g          295 t
ORIGIN      
        1 agaaggcaag tggctggctt ggctgggaat caccgcggag caactggcgc acatttcggc
       61 tttgcaggaa attgccgcgg atatcaggca ggccggattc aaacatgcgt tgcttctcgg
      121 catgggaggt tccagactgt gccctgaagt gttgcgcatg acttttggga aaatcgatgg
      181 gtaccctgaa ttgcacgtgc tggattccac cgatcccgcg cagatcaaag ccatcgaaag
      241 caaagtcgat ctctccagca cggtgtgtat tgtgtccagt aaatccggga gcacgctcga
      301 gcccaacatt tacaagcagt atttcttcga gcggataaag gaaaaagtcg gcgaaggcga
      361 agttggcaaa cgcttcatcg cgattaccga ccctggctca aaaatgcagg aagtggcgga
      421 gcgcgacaaa tttcgcaaaa tcttcatggg cgtaccgggt attggcgggc ggtattctgc
      481 actgtcaaat tttgggatgg ttccggcagc gatcatggga atcgatgtcg cgaaatttct
      541 aaacaacaca gcggagatgc tgaaggcttg cggcgcagcg gtaccggcgg attctaatcc
      601 gggagcgttg ctgggtatgg ttctgggcgt ggcggctaat cacgggcggg acaaggtgac
      661 tatcgttgct tcaccgggga tttccgacct tggggcctgg ctggaacaat tgctcgctga
      721 atccaccggc aaagtgggca aaggtattat tcccgtggac cgcgagccgc tcgcgagtcc
      781 ggacagttac ggcgacgatc gcgtcttcgc ttatttgcga ttggaaacgg cgccggataa
      841 atcgcaggac accgccattc aggcgctcga agctgctgga catccagtgg tgcgcatcgc
      901 ggtcgataat atttacaatc ttgggcagga atttttccgt tgggaaattg ccacggcggt
      961 tgcaggttcc atcattggca tcaacgcttt caatcagccg gatgtggaag ccagcaagat
     1021 cgaaactaag aaattaacaa gcgagtacga aaaatccggc aagttgccgg atgaaagccc
     1081 gttctttgat ggcgacggca tcaagctgta cgccgacgaa aaaaatatcg aagtgcttaa
     1141 gggaaagccg cggcttgcgg aagtattgaa agcgcatctc gggcggttgc gcgcgggcga
     1201 ttacttcgcg gtgctcgggt acattacgat gaacgacgct aatgagaaag ccatgcaagg
     1261 gatacggctc gccgttcgcg accggaagaa agtcgcgacg gtgctcggat ttgggccgcg
     1321 cttcctgcac tcgacggggc aggcttacaa gggcgggccg aacagtggcg tgtttctgca
     1381 gatcacctgc gatgacgc
//
LOCUS       DATCBB010000343         1050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_205585, whole genome shotgun sequence.
ACCESSION   DATCBB010000343 DATCBB010000000
VERSION     DATCBB010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205585"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VII37_03820"
     CDS             <1..292
                     /locus_tag="VII37_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068882.1"
                     /translation="EASAILNELKKYDPTLYQKPRWLLLNKVDLLENREEKVAAFLKE
                     FTDFDRYFVISAINGEGCKELTYAIMDHLLAMNELERQAAADSAQETDADFI"
     gene            488..>1050
                     /gene="proB"
                     /locus_tag="VII37_03825"
     CDS             488..>1050
                     /gene="proB"
                     /locus_tag="VII37_03825"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY5068883.1"
                     /translation="MKTVLTQCKRIVVKVGSSLVTNQGAGLDLSALGNWARQIAALNT
                     SGHEVVLVSSGAIAEGMQRLGWSTRPVSIHDLQAAAAVGQMGLVQAYESCFRQHGLHA
                     AQVLLTHADLADRERYLNARATLTTLLSLRVIPIINENDTVVTDEIKFGDNDTLGALV
                     ANLIEGDALIILTDQRGLFTADPRHDPT"
BASE COUNT          256 a          276 c          282 g          236 t
ORIGIN      
        1 tgaagcgagc gcgatactca acgaacttaa aaaatacgac ccgaccttgt atcaaaaacc
       61 gcgctggttg ctgctaaata aagttgatct gctggaaaat cgcgaagaaa aagtggcggc
      121 ttttctcaaa gagtttaccg actttgaccg ttactttgtg atctcggcga tcaacggcga
      181 gggctgcaag gaattgactt atgcgatcat ggatcatttg ctcgcgatga acgaacttga
      241 acggcaagcg gcagctgaca gcgcgcagga aaccgacgca gatttcattt agcgcagtac
      301 ctcataatta atggcgcatt ctgttgctcg cgcaaacgcg cgaagaaaac acaaaataag
      361 ccagcgaata atttcttcaa tgtcttcccg gtagttgcaa aaggcgaatc tgctttctga
      421 tttttttgcg tctttgcgtc ttcgcgggag gctggttttt tgatgggaat tccaatttag
      481 agcacacatg aaaaccgtat tgacccaatg caagcgtatc gtcgtcaagg taggcagcag
      541 cctggtgacg aatcaaggtg cgggactgga tctcagcgcg ctgggcaatt gggcgcgcca
      601 gatcgctgcg ctcaacacca gcggacatga ggtggtgctg gtgtcgtccg gcgcgattgc
      661 agaaggcatg caacgcctgg gctggagcac ccgtcctgtt tcgatacacg atttgcaggc
      721 cgctgcggca gtcggtcaaa tgggtctggt gcaagcctac gaaagctgtt tccgccaaca
      781 tgggctacat gccgcgcaag tcctgctcac gcatgccgat ctggccgatc gcgagcgtta
      841 cctgaatgct cgcgccacgc tgaccacgtt gttatcgctg cgtgtcatcc ccatcatcaa
      901 cgagaacgat actgtcgtca ccgacgaaat caagttcggc gacaacgaca cgcttggtgc
      961 gctggtagcg aaccttatag aaggcgacgc gctgattatc ctcaccgatc aacgcggcct
     1021 gtttaccgct gatccccggc acgatccaac
//
LOCUS       DATCBB010000344         1889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_43903, whole genome shotgun sequence.
ACCESSION   DATCBB010000344 DATCBB010000000
VERSION     DATCBB010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43903"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..>1889)
                     /locus_tag="VII37_03830"
     CDS             complement(147..>1889)
                     /locus_tag="VII37_03830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY5068884.1"
                     /translation="SDQQKVGDFYATCMDASAIESAGIKPIAPQVDAINAMNDRNALL
                     AEVAALHNDGVQALFDFSSTQDFADSTKVIGDADQGGLGMPDRDYYTRDDERSKQLRT
                     DYVAHIAKMFQLAGDPAEKASAEAQTVMNIETALAKASLTNVQLRDPKTNYHKLSTAE
                     LKQLTPDWSWDSFFADVHLPGLPETNIGQPDFFKEMDHQLTATPLSDWKIYLRWHVLH
                     NNAPALSDAFVQENFNFYGKRLSGTAELQPRWKRCVQSVNRNIGEALGKVYVEKYFPP
                     EAKAHAREMVQNLISALRSDIPTLSWMTPETKKAAIEKLEAFNIKIGYPDKWRDYSKL
                     NIERTSYAVNLRRASAFENARELAKIGKPVDRNEWGMTPPTVNAYYNPTMNEIVFPAG
                     ILQPPFYDPKADDAINYGGMGAVIGHEISHGFDDQGSQFDGQGNLRNWWTADDRKNFD
                     ERATCVEKQFDTYEVEPGLHQNGKLVLGESIGDLGGLSISYAAYEKSLEGKPRPKDLD
                     GFTPEQRFFLGWAQVWGTNQRAEAARLQANTNPHPLARFRGNGPLSDMAAFAQAWGCK
                     KGDPMVREQACKIW"
BASE COUNT          385 a          455 c          593 g          456 t
ORIGIN      
        1 tggcaaaatg gagagccata gagcggccga gcccagtacc gccgccggtc aggaaaatag
       61 ttttggattt cagcaggtct gattggaaca agagtggtcc tcaagcattc caaagcgtaa
      121 aaaacttgac ttgaaaacct gtggaactac cagattttgc aggcttgttc gcgcaccatg
      181 gggtcgcctt ttttgcagcc ccaggcctgt gcgaaagcag ccatatctga caaggggcca
      241 ttgccgcgga aacgcgcgag tggatgtgga ttggtgttgg cttgcaagcg cgctgcttcg
      301 gcacgctggt tagttcccca gacttgcgcc cagcccagga agaagcgctg ctcgggagtg
      361 aagccgtcga gatctttggg acgcggcttc ccttccaggg atttttcgta ggctgcgtat
      421 gagatggaga ggccacctag atcgccgatg ctttcaccga gcacaagctt gccgttctga
      481 tgcaggccgg gctcgacttc gtaggtgtcg aactgttttt cgacgcaagt ggcgcgctca
      541 tcaaaatttt tgcgatcgtc ggcggtccac cagttacgca gatttccttg gccgtcgaat
      601 tgcgatccct gatcgtcgaa accgtgggaa atttcgtgac cgattaccgc gcccatgccg
      661 ccgtaattga tggcgtcatc ggctttggga tcgtagaacg gaggctgcaa gattcccgcg
      721 gggaagacga tttcgttcat ggtggggttg tagtaggcat tgacggtggg cggcgtcatg
      781 ccccactcgt tgcgatccac cggcttaccg attttggcaa gctcgcgagc attttcaaaa
      841 gcagaggcgc ggcgcaagtt gacggcgtaa gaagtgcgtt cgatgttgag cttggaataa
      901 tcacgccact tatcggggta gccgatttta atgttgaagg cttccagttt ctcgatggcg
      961 gccttttttg tttccggagt catccaggaa agtgtgggga tgtcggagcg aagagcagag
     1021 ataagatttt gcaccatctc gcgagcgtga gcttttgctt ccggcggaaa gtatttttcc
     1081 acgtagacct tgccgagggc ttcgccgatg ttgcgattga cggactgcac gcagcgcttc
     1141 cagcggggct gaagctcggc ggtgccggaa aggcgcttgc cgtagaaatt aaaattctcc
     1201 tgcacgaagg cgtcgctgag tgcgggggca ttgttgtgga gcacgtgcca gcggagataa
     1261 attttccagt cggataaagg cgtcgcagtg agttgatgat ccatttcctt gaagaaatcg
     1321 ggctggccga tgttggtttc gggaaggccg ggaagatgga cgtcagcaaa aaaggaatcc
     1381 cacgaccaat cgggcgtcaa ttgtttcaat tccgcggtgg aaagtttgtg gtaattcgtt
     1441 ttgggatcgc gcagctgtac gttcgtcaaa gaagccttgg caagagcggt ctcgatgttc
     1501 attaccgttt gggcttctgc ggaggccttt tccgcgggat caccagcaag ctgaaacatt
     1561 tttgcgatgt gcgcgacgta gtcggtgcgg agttgtttgg aacgctcgtc atcgcgtgtg
     1621 tagtaatctc ggtcgggcat gcccaatcca ccctgatcgg cgtcgccgat caccttggtg
     1681 ctgtcggcga agtcctgcgt ggaactgaaa tcgaagagcg cctggacgcc atcgttatgg
     1741 agcgcggcaa cttcggcgag taaggcgttg cggtcattca tggcgttgat ggcgtcgact
     1801 tgaggagcga tgggcttgat gcctgcggac tcgatggcgc tagcgtccat gcaagtggcg
     1861 tagaagtcgc ctactttttg ctggtccga
//
LOCUS       DATCBB010000345         1410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_303172, whole genome shotgun sequence.
ACCESSION   DATCBB010000345 DATCBB010000000
VERSION     DATCBB010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303172"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..225
                     /gene="yacG"
                     /locus_tag="VII37_03835"
     CDS             52..225
                     /gene="yacG"
                     /locus_tag="VII37_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY5068885.1"
                     /translation="MRIFHFAANRCRERDLGNWASEKYVVSEPIFNEDASEEGDRKTI
                     ILDLPDRTGEAEH"
     gene            516..1097
                     /locus_tag="VII37_03840"
     CDS             516..1097
                     /locus_tag="VII37_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY5068886.1"
                     /translation="MTPSTETSIAHSTRGPKPHISLLLATWFGLGYLPKAPGTWGSLG
                     GVAVSLLITWVSFRRHFFILNMEREVHFWTTVWLPPPYHLAVVVLIAAIGVIVATHAA
                     RYAQIEDPQWVVIDEVSGQLLTYYLFFWLLPLNWKSWLLGFILFRAFDIWKPFPARQL
                     EHLPGGWGIMADDWMAGIYAALVLRVALHFRVL"
     gene            1094..>1410
                     /locus_tag="VII37_03845"
     CDS             1094..>1410
                     /locus_tag="VII37_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY5068887.1"
                     /translation="MKLGLGGAVEKNGNPRIERVAPTAAIPGGEITIHGSGFVSRTQA
                     RPLVRFGEAEASLALSSGKRLIARVPHGATGGIVRVTTAEHESQPYPVAIGLQIADNL
                     HPVG"
BASE COUNT          299 a          449 c          353 g          309 t
ORIGIN      
        1 acatgaaaca caaatgcccg atatgtaaaa ccgccaccga ttccgcggta catgcggatt
       61 ttccattttg cagcgaaccg ctgccgcgaa cgcgacctcg gaaattgggc cagcgaaaag
      121 tacgtcgtct ccgaaccgat cttcaacgaa gacgccagcg aagaaggcga ccgcaaaacg
      181 atcattctgg atttgcccga ccgcaccggc gaagccgaac attaaatcct gcgccacagc
      241 gtcaatgttc gtgatcgcca cgatccaaaa gtatgcgtag ggtgccccac cctcagattt
      301 ttgaagggtg ggtcttgtaa cgtggaaaca tcgcccaact aaaccgcggg tgcgcaaatg
      361 tccgcttctt gacgtcatct tcgacgctat atgatcgtga tggccgtggc ctgtaaggcg
      421 ttacagactg ccgggccatt agctgcttca ccaccaaacc atttctcgct gattaaatct
      481 gactctcatc tacctgctgc gataccctta acgcaatgac gccgtccact gaaaccagca
      541 ttgcgcactc aacccgcgga ccaaaaccgc acatctcgct tctgctggcg acctggtttg
      601 gactcggcta tttgcccaaa gctcccggaa cctggggctc actaggcggc gtggccgtct
      661 cactgttaat cacttgggtt tcgtttcgcc gccacttttt catcttaaac atggagcgcg
      721 aagtacattt ctggacgacg gtttggcttc cgcctccgta tcaccttgca gttgttgtac
      781 taatcgctgc gatcggcgtg atcgtcgcca cccacgccgc ccgttatgcg caaatcgaag
      841 atcctcaatg ggtcgtcatc gacgaagtct ccggccaact cctcacctac tatttatttt
      901 tctggctcct gcccctcaac tggaaaagct ggctcctcgg ctttatactt tttcgtgcct
      961 tcgacatctg gaagccgttc cccgcacggc aactggagca tctgcccggc ggctggggaa
     1021 tcatggccga cgattggatg gcaggcatct acgctgcatt agtcttacgt gtcgcgttac
     1081 acttcagagt gctatgaaac taggcttggg cggggcagta gaaaaaaacg gcaatccacg
     1141 catcgaacgc gttgcgccca ccgcggcgat tcccggcggc gaaattacca ttcatggtag
     1201 cggcttcgtt tcccgcactc aggcgcgtcc gctggtgcgc tttggcgagg ccgaagccag
     1261 cctcgcgctc tctagcggca agcgcctcat cgcccgcgtg cctcacggag ccaccggcgg
     1321 catcgtgcgc gtcaccaccg ctgaacacga gagccagccc tatcccgtag ccatcggcct
     1381 ccaaatcgcc gacaacctgc atcccgtcgg
//
LOCUS       DATCBB010000346         1260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_75563, whole genome shotgun sequence.
ACCESSION   DATCBB010000346 DATCBB010000000
VERSION     DATCBB010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75563"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VII37_03850"
     CDS             <1..871
                     /locus_tag="VII37_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5068888.1"
                     /translation="LDVDKFAPRLSFFFNAHNNFLEEVAKFRAARRMWARLMREKFKA
                     ENPRSWMLRFHAQTAGSTLTAQQPENNIARTALQALAAVLGGTQSLHTNSYDEALALP
                     TEQAARIALRTQQIVAFESGVPQTVDPFAGSYHIESLTNEIEQRAADYLEKIDALGGM
                     LRAIERGYVQQEIQNAAYEYQQKVDRNEAMVVGVNRFASEKSDGEDVPIQLQRVDESL
                     ERRQVERLQALRERRNPQEWSASLRQVEDAARNGSNLMPCIVAAVEAFATVGEISDTM
                     RKVFGEYKEAVVI"
BASE COUNT          297 a          341 c          355 g          267 t
ORIGIN      
        1 ccttgatgta gacaaatttg ctccacggct gtcatttttt ttcaacgcgc acaataactt
       61 tctggaggaa gtcgccaaat tccgcgctgc gcgtcgcatg tgggcccgcc tcatgcgaga
      121 aaaatttaaa gcagagaatc cgcgttcgtg gatgttgcga tttcatgccc agactgcggg
      181 atctaccctg accgcgcagc agcccgaaaa caatatcgcg agaacggctt tgcaggcgct
      241 ggccgccgtc ctgggaggaa ctcagtccct gcatacgaat tcctacgatg aagccctggc
      301 gcttcccacc gaacaggccg cgcggatcgc gcttcgcact cagcagattg tcgccttcga
      361 gtcgggcgtt ccccagaccg tcgatccatt cgcgggttca tatcacatcg agtctcttac
      421 gaacgaaatc gaacaacgcg ccgcagatta tctggagaag atcgacgcct tgggcggaat
      481 gcttcgcgcc atcgaacgag gctacgtgca gcaggaaatt caaaatgccg cgtacgagta
      541 tcaacaaaaa gtggaccgca acgaagccat ggtggtcggg gtaaatcgat ttgcgtctga
      601 gaagagcgat ggcgaagatg ttcccattca acttcaacgt gtcgatgaat ccctggaacg
      661 caggcaggtt gaacgccttc aggcgcttag agaacgccgg aatccgcagg agtggtcggc
      721 atcgttaagg caggtcgaag atgcggcccg caacggatca aatctgatgc cgtgtatcgt
      781 cgccgcagtc gaagccttcg ccaccgtagg agaaatctcc gacaccatga gaaaggtatt
      841 tggagaatat aaagaggcgg tggtgattta aggcgcatct ggcttcgcca gcccgaagtt
      901 cggcaagcgg gaaataagcg agcccggaac ggacgaaaga ggtggcttca ttccgccact
      961 ttgtaatcgc aggcctggcg gcagtcggcg caataataca gtccctcttc gcacagcctg
     1021 acgttaagct ggctttcgca ggagcaacaa tacttctcac atccacattt ctgttcgggc
     1081 atctcgcact gcgcacagag tacttttgtt ttggtgctca tccacgaacc ctatcgcgag
     1141 cggcgattac tggaaagtgc cgttggtaac cgggagcctg cccggctcag gttgcggaaa
     1201 aactcattct cgcggggtgg atttattagc ggcgagtttt gccggagctc gtttaggcag
//
LOCUS       DATCBB010000347         1133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_44225, whole genome shotgun sequence.
ACCESSION   DATCBB010000347 DATCBB010000000
VERSION     DATCBB010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44225"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VII37_03855"
     CDS             complement(<1..1001)
                     /locus_tag="VII37_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5068889.1"
                     /translation="MSSEAAVTGAPRLRRALTLWNLVIIGMVIVQPIAPMGIYGVISN
                     KAGGHVVTTILIAMVAMLFTAISYGKMARAYPSAGSAYTYVGREIHHGFGYLTGWAMV
                     MDYILNPLICTIICSKLTQNILPGMPYWVLAIFYAGFFTLLNLRGVKTSARINDVLAA
                     GMSIVVVIFFVFIIRFLWGMHVYGARFFTMPFYNPATFSFSGVFQGTSIAVLTYIGFD
                     AISTFSEEVENPRRNILLATVLVCVITGVLSSLEVYAAQLVWGSAPFPSDKVESAFPM
                     VAQQVGGFFLFQLLNFTILIANIGSGIGSQLAAARLLYGMGRNDALPKGFFGKITASR
                     "
BASE COUNT          302 a          313 c          285 g          233 t
ORIGIN      
        1 cggctggcgg ttatctttcc aaaaaatccc ttgggcagcg cgtcgttacg ccccattcca
       61 tacagcaacc gcgccgccgc cagttgcgat ccgatgcccg agccgatgtt ggcgataaga
      121 atcgtaaaat tcaacagctg gaaaagaaaa aatcctccga cctgctgcgc caccatggga
      181 aaagcggatt ctactttgtc gctagggaat ggcgcggagc cccacaccaa ctgtgcggcg
      241 tacacttcca gcgatgatag tacgccggtg atcacgcaga ccagaacagt tgccagcaaa
      301 atattgcgcc gcggattttc cacttcttcc gaaaatgtcg agatggcatc aaagccgatg
      361 tacgtcagca ccgcaatgga ggtgccttga aaaactccag aaaaactgaa cgtggccggg
      421 ttgtagaacg gcatggtaaa aaatctcgcc ccgtacacat gcattcccca taaaaaccgg
      481 ataatgaaga cgaaaaatat caccaccact atggacattc ccgcggccag cacgtcattg
      541 atacgcgccg aggttttgac tcctcgtaaa ttcaacaatg taaaaaaccc ggcataaaaa
      601 atggccagca cccagtacgg catccctgga agaatgtttt gcgtaagttt gctgcagatg
      661 atggtgcaaa tcagcggatt caaaatgtaa tccatcacca tggcccatcc ggttaaataa
      721 ccgaacccgt gatgaatctc gcggcccaca taggtataag cggagcccgc gcttggataa
      781 gcgcgcgcca tttttccgta gctgatggcc gtaaataaca tggccaccat ggcgatcaaa
      841 atggtggtga ctacatggcc gccggccttg ttgctgatca ctccgtagat gcccatgggc
      901 gcaatgggct gcacgatcac catgccgatg atcaccaggt tccagagtgt gagggcgcgc
      961 cgcaatcgcg gtgcgccggt gacggcggct tcactactca aaagactcct cctctaagcg
     1021 gtgcggaacg acctctgctt tgttcatggt gcttgaacac cgagagaaac gcgttgcggc
     1081 gagtcgctct attgaatcgt ttcaatggca gtaactctgc aagaacttcg caa
//
LOCUS       DATCBB010000348         1562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_141347, whole genome shotgun sequence.
ACCESSION   DATCBB010000348 DATCBB010000000
VERSION     DATCBB010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141347"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VII37_03860"
     CDS             complement(<1..190)
                     /locus_tag="VII37_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068890.1"
                     /translation="MTSPETEPSYFDEVKSLWSRRKRIWDLIKHANKLRIWGLPILVV
                     HAAVQTGMALLWGKLFDKV"
     gene            complement(194..>1562)
                     /locus_tag="VII37_03865"
     CDS             complement(194..>1562)
                     /locus_tag="VII37_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5068891.1"
                     /translation="PALQPAPSEITDGWEMSWIAGVVMLVLLFGAFFAMTKYEVPLPL
                     AMTLVSLLFLVIQWENAPHLLRLGFEHYAPITVLFTAVAIPAHMIERSQGFNWIAAWL
                     GHQLGKIRLRHPRLTTPLLVVILLLATYVTAGLMHNVTSILIMTPVIIRLCASYQVPS
                     RWILSAALIASNLGGFSTRWGDTPNIIESATWGLTAGNFMREVLPANLIIIAVLITVA
                     TLLTQRGISRRSREAGKTQVPFYTLQVAQTAADWKKAKAELAIDRRLLWTGLVLLVLF
                     IVLHVIFTTLAIVIGAITIVVAVLMERNKSRYHTLTSLGFDVYLAFAAIFVLATCVSH
                     SWIGHTLYNQLIAAKAAPWAIAVTGYFGTTFTEAASWATAVSPAIQQLDSSHAAAWAL
                     GGGICAGSSSLVTAASAGIILVAESGRFKDPEHAINFRRYIVFGVPFSLFMLGFYSLY
                     FTFVR"
BASE COUNT          391 a          432 c          439 g          300 t
ORIGIN      
        1 tgactttatc gaagagcttt ccccagagaa gcgccatgcc ggtttggacg gcggcatgga
       61 ccaccaagat cggaaggccc cagatgcgaa gtttgttcgc gtgcttgatt aagtcccaaa
      121 tccgtttgcg gcgcgaccac aaacttttga cttcatcgaa atatgaaggc tcggtttcgg
      181 gggatgtcat gattcaccgt acgaacgtga aataaagact gtagaaccca agcatgaata
      241 gcgagaatgg cacaccgaac acgatgtagc ggcggaaatt gatcgcgtgt tctggatcct
      301 taaaacgacc gctctctgcc acgagaatga tccccgctga tgcggcggtg acgagcgaac
      361 tgctgccggc gcaaattccg ccgccgagcg cccaggccgc ggcgtgcgat gaatcgagtt
      421 gttggatggc cggcgaaacg gctgtggccc acgaagctgc ttcagtaaag gtggttccaa
      481 aataaccggt aacggcaatc gcccacggcg ccgccttcgc ggcaattagt tgattgtaga
      541 gcgtatgccc aatccatgaa tgcgaaacac aggtggcgag aacaaatatc gcggcgaagg
      601 cgagatagac atcgaatccc agcgaagtca gcgtgtgata tcgagatttg tttcgctcca
      661 ttagtacggc taccacgatc gtgatcgcgc cgatcacgat ggccagtgtc gtaaaaatca
      721 catgcagcac aatgaaaagg acaagcaaca cgagtcccgt ccacagcaag cggcggtcga
      781 tagccagctc cgccttcgct tttttccaat ctgctgccgt ctgcgccacc tgcaaagtgt
      841 aaaagggtac ttgagtcttt ccggcttcgc ggctgcgacg gctgatgccg cgctgcgtga
      901 gcagcgtggc cacagtgatt agcacggcga tgatgatcaa attcgcgggc aatacttcgc
      961 gcatgaagtt tccggccgtc agcccccacg tcgcggattc gatgatgttg ggagtatctc
     1021 cccagcgcgt ggagaatccg ccgaggttgc tggcaatcaa ggccgcactc aggatccacc
     1081 gcgacggaac ctgatacgaa gcacagagcc tgatgatcac cggcgtcatg attaagattg
     1141 aagtgacgtt gtgcatcagt cccgcagtta cataagtcgc cagcagcaaa atcacgacta
     1201 acaacggcgt cgtcaggcgc ggatggcgca acctgatttt gccgagctga tgtcccagcc
     1261 acgcggcaat ccagttgaaa ccctgagagc gctcaatcat gtgcgcggga atagccaccg
     1321 cagtgaacaa gactgtaatc ggcgcgtaat gctcgaagcc cagtcgaaga aggtgcggcg
     1381 cgttttccca ttggataacg aggaacagta agctcacgag cgtcatcgcc aatggcagcg
     1441 gcacttcata cttcgtcatc gcgaagaatg cgccgaacaa cagcaccagc atcacaacgc
     1501 cggcgatcca gctcatctcc cagccatcag taatctctga aggagcgggc tgcaaagcag
     1561 gg
//
LOCUS       DATCBB010000349         1608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_237776, whole genome shotgun sequence.
ACCESSION   DATCBB010000349 DATCBB010000000
VERSION     DATCBB010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237776"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VII37_03870"
     CDS             <1..909
                     /locus_tag="VII37_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY5068892.1"
                     /translation="AKKLAAMSQMDVDKMFQQAQSRDFKPVELPVRLKAHVVSTVRPF
                     NSRNVLAMLPGSDPNRRNETVMYTAHYDHLGIDTSAKGDNIYNGAVDNGTGCGIILEL
                     ARAWSQSEQSGQQPPPRSILFAAVTAEEQGLLGSEFLGKHFATPVGKVTLDLNYDAFA
                     PIGDPEEVEVSGAERTTFYPLVEKTAQKYGFAIRPDAQPEAGHYYRSDHFSLARVGIP
                     SFSISEGMKFKGHDEAWGRAQAKDYVEHHYHQPSDEYRPEMDFTGLAKVAQFGYDLGL
                     QAATQPDLVQWQSGDEFESARKKAGQ"
     gene            complement(922..1047)
                     /locus_tag="VII37_03875"
     CDS             complement(922..1047)
                     /locus_tag="VII37_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068893.1"
                     /translation="MGTHEGTPYIVSELLRGETLRHQTGKYFYLRGWAVEDSVLV"
     gene            complement(1166..>1608)
                     /locus_tag="VII37_03880"
     CDS             complement(1166..>1608)
                     /locus_tag="VII37_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068894.1"
                     /translation="KELETGLAQIPEDVPGGFSPAKTILQLADTVLSARIAEAQGDHQ
                     AAIATWHKAMEMQDQLAYDEPPDWYYPVRESLGGALLRAGQVDEAEKIFREDLLQNPR
                     DGRSLYGLMMSLAAQKKTVDTEWARREFEEAWKNADTKLRVEDL"
BASE COUNT          387 a          506 c          366 g          349 t
ORIGIN      
        1 gccaaaaagc tggcggccat gtcgcaaatg gatgtcgaca agatgtttca gcaagcgcaa
       61 tcgcgcgatt tcaagcccgt ggaattgcct gtgcgtctga aagcccacgt cgtcagcacc
      121 gtccgcccat ttaattccag aaacgttcta gccatgctgc cgggcagcga ccccaatcgt
      181 cgcaacgaaa ctgtgatgta caccgcgcac tacgatcacc tgggcatcga caccagcgcc
      241 aagggcgaca acatttataa cggcgcggta gacaacggca caggctgcgg catcatcctc
      301 gaactcgccc gcgcttggtc gcagtctgaa caatccggcc agcaacctcc gccgcgatcc
      361 attttattcg ccgcggtaac cgccgaagaa caaggcctgc tcggctcaga attcctcggc
      421 aaacatttcg caactccagt gggaaaagtg acgctcgatt taaattacga cgccttcgcc
      481 cccatcggcg acccggaaga agtagaagtc agcggcgcag aacgcaccac cttttatccg
      541 ctagtagaaa agaccgccca aaaatatggc ttcgctattc ggccggacgc gcaaccggaa
      601 gccggccatt attaccgatc tgatcatttc agcttggccc gcgtgggcat cccttcgttt
      661 tcaatcagcg aaggaatgaa attcaagggc cacgacgaag cctggggcag agcgcaagcc
      721 aaagattacg tcgagcatca ttaccaccaa cccagcgatg aataccgccc cgaaatggac
      781 ttcacaggtc tcgctaaagt tgcacagttc ggctacgacc tgggactgca agccgcaacg
      841 caaccggatt tggtgcaatg gcaatcaggc gacgaattcg aatccgcgcg aaaaaaagcc
      901 ggacaataat tcactcacat ctcaaaccaa aacagaatct tcaactgccc atccgcgcaa
      961 ataaaaatat tttccggttt gatgccgcag cgtttcgcca cgaagaagtt cgctgacaat
     1021 gtacggggtg ccctcgtgcg tgcccacatc agagatagtc agaatgtttg cgtgatttaa
     1081 ctgtccgcac acaagggaag ctggtataaa ttgtggctct tacttcttgg ctctaaatcc
     1141 atttccacaa attgccgtca atcgttcaca aatcttccac gcgcaacttc gtatccgcat
     1201 ttttccaagc ctcttcaaat tcccgccgcg cccattccgt atctactgtc tttttctgcg
     1261 ccgccagact catcatcaat ccatagagtg atctgccgtc cctcggattt tgcagcagat
     1321 cttcacgaaa tattttctct gcttcatcca cctgccccgc ccgcagcagc gccccgccca
     1381 gcgattcccg caccgggtaa taccaatccg gtggctcgtc gtacgccagt tgatcctgca
     1441 tctccatcgc tttgtgccat gttgcaatcg ctgcttggtg atctccttgg gcttccgcga
     1501 tccgcgccga aagtaccgta tccgccagtt gcagtatcgt tttcgccgga ctgaaccccc
     1561 ccggcacgtc ctcgggaatc tgcgctaaac ctgtctccaa ctccttct
//
LOCUS       DATCBB010000350         1636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_141479, whole genome shotgun sequence.
ACCESSION   DATCBB010000350 DATCBB010000000
VERSION     DATCBB010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141479"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VII37_03885"
     CDS             complement(<1..1023)
                     /locus_tag="VII37_03885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY5068895.1"
                     /translation="MPADSVLEKPLPNNLDAERSVLGAILLDNNALNPAIEHLRPEDF
                     FMEQHRRVFTQMIALGESQQAIDLVTLTEELHRRGDLEASGGAPYLAALADGMPRVSN
                     VEHYARIVKEKAMLRNLIHATHNIQQRAFEGEDGADAILDNAESSIFALAEDRVRAGL
                     IPVKDIVRDNFERLERIFREGKSITGVATGYGELDKLTSGLQPSELLILAARPSQGKT
                     ALALNLAENISIRAGHPAAIFSLEMSKESLLQRLVASVAQIDAHKFRTGHLSKEDWRR
                     MTEALGVISAAPLWIDDAGSISVLEIGAKARRLKRDKGLSLLIVDYLQLITARGRFSN
                     RNEEVAS"
     gene            complement(1359..>1636)
                     /gene="rplI"
                     /locus_tag="VII37_03890"
     CDS             complement(1359..>1636)
                     /gene="rplI"
                     /locus_tag="VII37_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY5068896.1"
                     /translation="EMEAAQKQAAALNGVELSFTRKTGENDQLFGSVTTGDIADALES
                     KGFKIDKRQIHTAEPIKALGDFRATVKVFRDITAEVKIQVQKEGQQA"
BASE COUNT          365 a          503 c          388 g          380 t
ORIGIN      
        1 gctagccact tcctcgttgc gattcgagaa tcgtcctcgc gcggtgatga gctgcagata
       61 atccacgatc agtagcgaaa gtcccttgtc ccgcttcagc cgtcgcgcct tcgcgccaat
      121 ctccagcacg ctgatcgatc cagcatcgtc aatccacaat ggcgccgccg aaattactcc
      181 cagcgcctcg gtcatgcgcc gccaatcttc cttgctcaaa tgcccggtgc ggaatttgtg
      241 cgcgtcaatc tgcgccaccg aggccaccag gcgctgcagc agcgactctt tcgacatttc
      301 caggctgaag atcgccgcag ggtgccccgc gcggatggaa atattttccg ccaggttcaa
      361 tgccagcgcc gttttcccct gcgatggccg cgccgcaaga atcagcaatt ccgacggctg
      421 cagcccggaa gtcagcttat ccagctcgcc gtacccggta gccaccccgg tgatgctctt
      481 gccttcgcga aaaatccgtt ccagccgctc gaagttgtcg cgaacgatat ccttaaccgg
      541 aatcaacccc gcgcgcacgc gatcttccgc cagcgcaaat atcgaagact ccgcgttatc
      601 cagaatggcg tccgcaccat cctcgccttc aaacgcgcgc tgctgaatat tgtgcgtggc
      661 gtgaatcaag ttgcgcagca tcgctttttc tttcacgatg cgcgcgtaat gctccacgtt
      721 cgatactcgc ggcatgccgt cggccagcgc cgcgagatat ggcgcgccgc ccgaagcctc
      781 tagatcgccg cggcgatgca gctcttccgt cagcgtaaca agatcgatgg cttgctggct
      841 ctcgcccaag gcaatcatct gcgtgaatac gcgacgatgc tgctccatga aaaaatcttc
      901 gggacgtaaa tgctcgatgg caggattcag cgcgttatta tccagcaaaa tagcgcccag
      961 gacggaacgc tcggcgtcca aattgttagg cagcggtttt tctaggacgc tatcagcagg
     1021 cataactcgg gttcgcctcg tgcttcgctt tatcttcgcc taggcgaatt tgtagcatgt
     1081 tcccgacccc tgaggcaagg aaaagaaatt ctcgagttgg tggacgccta cgacccgatc
     1141 ggattcgcac cgaatcgaaa taatttaatg aaacgcattg tgtactgaac tgaaaccatc
     1201 gtcaaccatc aatgcaatct agacctgtgc atcgcgtcga aaagtttgtg caaaactttt
     1261 ttgcagcaaa taattttctt gcacaaaacg tttttctata caaaaaaaaa gaggcacatc
     1321 taaatgtgcc tcttcgaatc gcgaaaccga aattaaaact acgcctgctg cccttccttc
     1381 tgcacctgaa tcttcacttc ggcggtaata tcccgaaaaa cttttaccgt tgcgcgaaaa
     1441 tctccgagcg ccttgattgg ttccgcggta tgaatctgcc gcttgtcgat cttgaaacct
     1501 ttgctctcca gcgcatcggc aatatcgcca gtcgttaccg agccaaataa ctgatcgttc
     1561 tcgccggttt tgcgcgtgaa gctcagctcc acaccattca acgcagcagc ctgtttctgc
     1621 gccgcttcca tttccg
//
LOCUS       DATCBB010000351         1056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_368104, whole genome shotgun sequence.
ACCESSION   DATCBB010000351 DATCBB010000000
VERSION     DATCBB010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368104"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VII37_03895"
     CDS             complement(<1..987)
                     /locus_tag="VII37_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068897.1"
                     /translation="MALMLTALLLTGCPKGKPDNNAGAKAEALKDYDTALDYYNKALQ
                     TSPTNTEYKLKAARARFEAAQWHVDQGRRFQEQANLELALAEFRKAQMIDPSSAVAQQ
                     EVKATTDLINAKNGITQNATPSGLSSEEPKLMSGPPRVQPLSRAPINMKATNDSKAVF
                     EAIGKLAGLTVIFDPDFTSRRISVELTNVTLEQALNITSLESKAFWRPVTSNIIFVVP
                     DQPQKRKDYEEEVVKTFYLRNTVLPQELTEIVTSIRQLLDLRRVQQINAQNAIVIRDT
                     PDRIMLAGKIMDDIDKAKPEVVIQVAVMEARRDRMRNVGITPPTSITATFANP"
BASE COUNT          183 a          297 c          311 g          265 t
ORIGIN      
        1 tgggttggca aatgtcgccg taatgctggt cggcggcgtg atgcccacat tgcgcatgcg
       61 gtcgcgccgc gcttccatca cggccacttg aatcaccact tccggcttgg ccttgtcaat
      121 gtcgtccatg atttttcccg ccagcatgat gcggtccggc gtgtcgcgaa tcacgatggc
      181 gttctgcgca ttgatttgct gcacgcggcg caaatcgagc agttgccgga tgctggtcac
      241 gatttcggtg agttcctgcg gcagcacggt attgcgcaga taaaacgttt ttacaacttc
      301 ttcttcatag tccttgcgct tttgcggctg gtcgggcacc acaaaaataa tattggatgt
      361 caccggacgc cagaatgcct tgctctccag cgaggtgatg ttcagcgcct gttcgagcgt
      421 cacgttggtc agctcgacgg aaattcgccg cgaagtgaaa tccgggtcga agatgacggt
      481 caggccggcc agcttcccga tggcctcaaa aaccgccttt gagtcgttcg tggctttcat
      541 gttgatcggg gcgcgcgaaa ggggctggac ccggggcggc ccggacatca gttttggttc
      601 ttccgaagat aaaccggacg gagttgcgtt ttgcgtgatc ccgtttttgg cgttgattaa
      661 atccgtcgtg gctttcactt cctgctgcgc cacggcgctg gacgggtcga tcatctgcgc
      721 cttgcggaac tcggcaagcg ccagctccag gttggcttgt tcctgaaaac gccggccctg
      781 atcgacgtgc cactgtgcgg cttcaaaacg ggccctagcc gccttcagtt tgtattcggt
      841 gttggtcggc gaggtttgaa gcgctttgtt gtaatagtcg agcgcggtgt cgtaatcctt
      901 cagagcttcc gctttcgcgc ctgcattgtt atccggcttg cccttggggc aacccgtcag
      961 gagcagggcg gtcagcatca gggccaatcc actcaaattc cgaaggttca tccgcgctcc
     1021 acctggtgca ttttcatcga gacagtctag caccgg
//
LOCUS       DATCBB010000352         1584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_368113, whole genome shotgun sequence.
ACCESSION   DATCBB010000352 DATCBB010000000
VERSION     DATCBB010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368113"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VII37_03900"
     CDS             <1..192
                     /locus_tag="VII37_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068898.1"
                     /translation="LQGLGEDYLKNYVQRVNAVTPDDVQHMTVKYIKPEQMTIVVVGD
                     KSKITEQLAPYAGLPESAK"
     gene            complement(214..1500)
                     /locus_tag="VII37_03905"
     CDS             complement(214..1500)
                     /locus_tag="VII37_03905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5068899.1"
                     /translation="MPFTFLWKSRKPATTVLLHALAFAGVLLAAYWLRGKSLQADTVL
                     QGYLQVITGLLAFVFAAVTLVRFQGTQDRISLILGVGFMVSGAVLTATSVLFFQLRDA
                     SLEFLWAPVAWWESRMEFALLLIVALLVERFLPRSLHPRRELVGALVTIISASYLITA
                     TLRRLPPEVSPHPFALFPSPQQLLPGAIFMASVIWYRRRLKSDSSAFDRAIYATAALN
                     LAAQCAASQSEHLLDAPFALAQGLTVMSYIVALGGALLDNARLFEQVRHLAVSDPLTG
                     LANYRRLLDVLEKETERSNRTGRPFSILLLDVDGLKKINDTHGHLVGSRAICRLADSL
                     RMNIRAVDTAARYGGDEFVLVLPEAREHDAHKVALRIHEVMRNDPEEPAVSASIGVST
                     YRGENGRIEKLLSEADKDLYRQKSSRQKRATAKTSG"
BASE COUNT          399 a          426 c          453 g          306 t
ORIGIN      
        1 ttacaggggc tcggcgaaga ctacttaaaa aattatgtac aaagagtgaa tgccgttaca
       61 ccggacgacg tgcaacacat gaccgtgaaa tacatcaagc cggaacagat gaccatcgtg
      121 gtggtcggcg ataaatcaaa aatcaccgaa cagctggcac catatgcagg actgccggaa
      181 tcagctaagt aagcactgct ctcagaaagc tagttagccg gaggttttgg cggtggcgcg
      241 tttttggcgg cttgattttt gccggtataa atccttgtcg gcttcggaca gcagcttttc
      301 tattctgcca ttttcgccgc gatacgtgga aacgccgatg cttgcggaga ctgcgggctc
      361 ttccggatca ttgcgcatga cctcatgaat ccgcaacgcc accttgtgag catcgtgctc
      421 tcgcgcttcg gggagaacca aaacaaattc gtctccgccg taacgcgcgg cggtatcgac
      481 ggcacggatg ttcattcgca agctatccgc caagcggcaa attgcgcggc tgccaaccaa
      541 atggccgtgc gtatcattga tttttttcaa gccgtcaacg tcgagcagca aaatggagaa
      601 aggcctgccg gtgcgattgc tgcgttccgt ttctttttcg agtacatcaa gaagccggcg
      661 atagtttgcc aaaccagtta gaggatcgct gacggcaagg tggcgcacct gctcaaacag
      721 cctcgcgtta tccagcaaag cgccgcccag cgcgacgatg taactcatca ccgtcaaacc
      781 ttgcgccaaa gcgaaaggcg cgtccagcag gtgttcggat tgagaagcag cgcattgcgc
      841 tgcgagatta agcgccgcag tggcgtaaat ggcccggtcg aaagcactcg agtcgctctt
      901 taaacggcgg cgataccaga tgacgctggc cataaaaatc gcgcctggga gaagttgttg
      961 aggactggga aacagcgcga acgggtgcgg tgaaacttcc gggggcaatc gccggagcgt
     1021 agcggtgatt aaataggatg cagaaattat ggtaacgagc gcgcctacca gttcccggcg
     1081 tggatgaagc gagcgcggca agaagcgctc cacaagaagc gccacaatca agagcagcgc
     1141 gaactccatg cggctttccc accacgccac cggagcccac aggaattcca gcgaggcatc
     1201 gcgaagctgg aagaaaagaa cactggtggc ggtcagtacc gcaccggaaa ccatgaaccc
     1261 cacgccgaga atcagcgaga tgcggtcttg cgtgccctga aagcgcacca gcgtgacagc
     1321 cgcgaaaaca aatgccagca agccggtaat gacctgcaag tagccttgca gcacggtgtc
     1381 agcttgtagc gacttgccgc gcagccagta ggcggccagc aatacgccgg cgaatgccag
     1441 ggcgtgaagc agcacggttg tcgctggttt gcgactcttc cacaaaaatg taaagggcat
     1501 gaagtttgcg gcggctttat tcttgccgtc aattcattag actatgcaga ggcgcagcgc
     1561 agtttcccta ctgtcaactg aagt
//
LOCUS       DATCBB010000353         4694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_303727, whole genome shotgun sequence.
ACCESSION   DATCBB010000353 DATCBB010000000
VERSION     DATCBB010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303727"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4546)
                     /locus_tag="VII37_03910"
     CDS             complement(<1..4546)
                     /locus_tag="VII37_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY5068900.1"
                     /translation="MAGSLASTPNTETAQQTSPSPQPVTPAEPALPRKDRLLVASRTL
                     LEELSGYDLSQVDPATSLLELGLDSLLLTQAAQVFHRKFGVNITFRQLMEELGSLQQI
                     AEHLDATLPADAFAPPAAQVAATAPAQQTAAAGNLGTANIPSSLLEQILQQQQALTQQ
                     VLQLMGRAPAQPAAMPSPVSNPMPAPSTTSSVPMVVKSEVKSHGPFKPIDRAADMAMT
                     AEQTAALKELIARYTKRTAKSKELAIKNRPILADPRSVGGFKQLWKDMVYPIFTTHSD
                     GSKVWDVDGNEYVDFVMGFGASIFGHRPPFVVEAVKKQLDLGFEIGPIQPIAGEVAEL
                     VREFTGMQRVAFTNTGSEAVLAATRVARTVSGRDKIAVFAGAYHGIFDEVLFRPLTIN
                     GETRTAASAPGVPASAVSQVIVLDYGNPQSLDILRARGSEIAAVLVEPVQSRRLDLQP
                     REFLQELRKVTKETGSALVFDEVVTGFRVEPGGAQAYFGVRADLATYGKVIGGGISIG
                     AVTGDPKYMDALDGGQWNYGDTSFPEVGVTFFAGTFVRHPLALAAAKAVLLHLKEKGP
                     DLQKNLTARMARLADQLRAIIDEFQAPYHISQFSSLMQLTFPSEQKYAALLHYMLRER
                     GIHMWDNRAFVLTTAHSEADLAKLLTAFRESLQAMVDAGFLPPPSSGSRKNTTVKTQT
                     SGAPAKEQRSVPVAAGLGAADQFPLTEAQKEIWLAAQMGGEAALGYNESLKLEFHGGF
                     DAELFRTAIQQIVQRHPILLASFSSDGQWQCIDPTVKLDVPLLDFSAKQKPTRELEKL
                     IETEVSSSFDLTRGPLVRVRIVRLSNDHHVVVWTAHHIVCDGWSGGLIVSELAKIYSA
                     LKQGTRPVLEAPESFRQYAITSQGDSEEARDASAYWSQQFAEIPTPLELPADRLRPAV
                     RTAQASTLKRDLPEPLHLLLKKTAGQQRTTLVVLLMAGLKTFLYRLTGQTDLVIGLGV
                     AGQAITGQSCLVGHCVNLLPVRSRLEPSGSFQQNLAAVKTSVLDAFDHNQSTIGSILQ
                     EIKVPRSPGRPPLVEVIFNIDKDPGAVEFEGLKFECERNPKRALHFDLFFNFVEGPRS
                     LYLECDYNTDLFDVSTIKRWLGHLQTLLESIAAAPTQAIDRLPILSEVERHEIVVRWN
                     DTAVKYDDVETLQNWFELQVERTPDALALTSEGENLTYVELNRRTNQLAHHLKKLGVG
                     PDVRVGVCAERSIEMVVALVGILKAGGCYMPLDPDYPSNRLALMLEDAQPPVILTQKA
                     LLPRLPAYEVPAICLDSEWDKIASESDANLGVQSTRNDAAYVIYTSGSTGKPKGVVNI
                     HEAIVNRLQWMQDAYRLTAADRVLQKTPYSFDVSVWEFFWPLVTGASLAMARPGGHRD
                     PEYLAQLIAQEKITTLHFVPSMLRLFLESAKLPKHSSIRQVFCSGEELGADLQRMFFA
                     ASDAELHNLYGPTEAAVDVTYWACRRDDQRSFVPIGRPIANTQIYILDHNLTPVPIGV
                     SGELHIGGIGLARGYLNRPEL"
BASE COUNT         1039 a         1362 c         1222 g         1071 t
ORIGIN      
        1 tcagttccgg acgatttaaa tagccgcgag cgagtccaat cccgccgata tgcaattcgc
       61 ccgaaacacc tatgggaacc ggagtcaaat tgtgatccag gatataaatc tgcgtatttg
      121 cgatcggccg gccgatggga acaaacgagc gttggtcatc tcgccggcac gcccaatacg
      181 tgacgtcgac ggcagcttcc gtcgggccat agagattatg caattccgca tcgcttgccg
      241 cgaagaacat gcgttgcaga tcggcgccga gttcctctcc gctgcagaac acctggcgaa
      301 tcgaagaatg ctttggcaat ttcgcgcttt ccagaaatag ccgcagcatg gatggcacga
      361 aatgcaaggt cgtaatcttc tcctgcgcga tcagctgcgc aagatattcc ggatcgcgat
      421 gcccgcccgg tcttgccatc gctaaactgg cgcccgtaac cagcggccag aaaaattccc
      481 aaacagaaac gtcgaagctg taaggagttt tctgcagcac tcgatcagcc gctgtcaacc
      541 ggtaagcatc ctgcatccac tgcaagcgat tcacgattgc ctcgtggatg ttaacgacgc
      601 ctttgggttt gccagtcgaa cccgaggtat aaattacgta cgccgcatcg tttcgagtcg
      661 attgcacgcc aaggttcgca tctgattccg aagcaatctt gtcccactca ctatccaggc
      721 aaatagcggg cacttcgtac gctggcaatc gcggcaacaa tgccttctgc gtcaaaatca
      781 ccggtggctg cgcatcctcc agcatcaatg ccagccgatt actcggatag tcggggtcga
      841 gtggcatgta acatccgcca gctttcaaga tccccaccag cgccacaacc atctcgatgg
      901 agcgttccgc acaaacgcct acacgcacgt ccggaccgac gcccaatttt ttcaaatgat
      961 gcgcaagttg gtttgttcgt cgattgagtt ccacataggt gagattctcg ccttccgacg
     1021 tcaacgccag cgcatcaggc gttctttcca cctgcaattc aaaccaattc tgtagcgttt
     1081 ccacgtcgtc gtatttcacc gcggtatcgt tccaccgcac cacaatttcg tggcgttcga
     1141 cttcggaaag gatcggcagc cggtctatcg cttgcgtggg tgcagccgcg atactttcga
     1201 gcagcgtctg caaatggccc agccatcgct taattgtgga aacatcgaaa aggtcggtgt
     1261 tgtagtcgca ctccagatat aacgaccgtg gcccttccac aaagttgaaa aacaaatcaa
     1321 agtgcagcgc gcgtttcgga ttccgttcgc attcgaactt caagccctca aattccaccg
     1381 caccgggatc cttatcaatg ttgaagatca cttctaccag cggcggtctg cccgggctgc
     1441 ggggtacttt aatttcctga agtatgcttc caatggtgct ctgattatga tcgaacgcgt
     1501 ctaaaacact cgtcttcact gctgcaagat tttgctgaaa gctgccgctg ggctcgagac
     1561 gcgagcgcac cggcaacaag ttgacgcagt gtcccaccag gcagctctgc cctgttatag
     1621 cttgacccgc cacgccgagc ccgatcacca aatcagtttg acccgttaag cggtatagga
     1681 aagtcttcag tccggccatt aacagaacaa ctaacgtcgt gcgctgttgc cccgcggttt
     1741 tcttcagcaa taaatgtagc ggctcaggga gatccctctt cagcgtagaa gcctgcgccg
     1801 ttcgcactgc tgggcgaagc cggtctgcgg gcaactccag cggtgttgga atttccgcaa
     1861 attgttgcga ccaataggcg gaagcgtcgc gcgcttcctc gctatcgcct tgactggtaa
     1921 ttgcgtattg gcggaaagat tctggagcct ccagaacggg cctagttcct tgtttcaatg
     1981 ctgaataaat tttcgccaac tcgctgacga tcaggccgcc tgaccatcca tcgcacacaa
     2041 tgtgatgcgc agtccaaacc accacgtggt ggtcattgga aagccgcaca attcgaaccc
     2101 gcaccagcgg cccccgggtg agatcgaagg aactcgacac ctccgtctca attaactttt
     2161 caagttcgcg tgtcggtttt tgtttggccg aaaaatccag cagcggcaca tccagcttta
     2221 cggtcggatc tatacattgc cattggccat ctgagctgaa gctggccagc aatatcggat
     2281 gtctctgaac gatttgctga atcgccgtgc ggaataattc cgcgtcaaaa cctccgtgaa
     2341 actccagctt caacgattcg ttatagccga gcgcggcctc cccgcccatt tgcgcggcta
     2401 gccagatttc tttctgcgct tcggtcaatg ggaactggtc ggctgcacca aggccagcgg
     2461 cgaccggtac gcttcgctgc tctttagccg gcgcgccgga agtctgcgtc ttgaccgtcg
     2521 tattttttct agaaccggaa cttggaggcg gcaggaaccc ggcgtcaacc atcgcctgca
     2581 aactctcgcg aaacgcagtc agcagcttgg cgaggtcagc ttcgctgtgc gcggtggtca
     2641 gcacgaacgc gcggttgtcc cacatgtgaa ttccgcgctc gcgcagcatg taatgcagca
     2701 acgccgcata cttctgctct gacgggaacg taagttgcat cagcgaactg aactgcgaga
     2761 tgtgataagg cgcctggaat tcgtcaatga tcgcgcgtaa ttgatcggca agccgtgcca
     2821 ttcgcgcggt caaatttttc tgaaggtcgg ggcccttttc tttcaaatgc aacaacactg
     2881 ctttagcggc cgccaatgcc agcggatgcc gaacgaacgt tccggcaaaa aatgttacgc
     2941 ccacctctgg aaacgaagtg tcgccataat tccattgtcc accgtcgagc gcatccatgt
     3001 atttaggatc gccggtgacg gcgccaatgg aaatgccgcc gccaatgact tttccgtacg
     3061 tcgccagatc ggcgcgcacg ccaaaatacg cttgtgcgcc ccccggctcc acgcgaaaac
     3121 cggtgaccac ttcatcaaaa accagcgccg agccagtttc cttggtcacc tttctgagtt
     3181 cttgcagaaa ttcccgcggc tgcaaatcca gtcgtctgct ttgcaccggc tcaaccagca
     3241 cagcggcgat ttccgagcca cgcgcgcgca aaatgtccag cgactgcgga ttgccgtaat
     3301 ccagcacgat gacttgtgaa accgcactgg ccgggactcc cggagcgctc gcagcggttc
     3361 gcgtttcgcc atttatcgta agcgggcgga aaagtacttc atcaaaaatt ccgtgatacg
     3421 cgcccgcaaa caccgcaatc ttgtcgcgtc cagatactgt tctggccacg cgtgttgctg
     3481 cgagcaccgc ttcagacccg gtattggtga acgccacgcg ctgcatcccg gtgaattcgc
     3541 gaaccagttc cgcgacttct ccggcgatcg gctgaatcgg tccgatttca aatcccagat
     3601 caagctgttt tttcaccgct tccacgacaa acggcggacg gtgcccaaaa atgctggcac
     3661 cgaaacccat aacaaaatct acgtactcgt tgccgtccac atcccagact tttgatccgt
     3721 ccgaatgtgt tgtgaaaatc ggatagacca tgtccttcca caattgttta aatccaccaa
     3781 ctgaacgcgg gtccgcaagg atgggccgat tttttattgc caattctttc gactttgccg
     3841 tgcgcttcgt atagcgcgcg atgagttctt taagcgcggc agtttgctcc gccgtcatgg
     3901 ccatgtccgc tgcacgatcg atcggtttga atggcccatg cgatttaact tcggacttca
     3961 ccaccatcgg aacggacgat gttgttgagg gcgcgggcat cgggtttgaa accggcgatg
     4021 gcattgctgc gggctgtgca ggcgcgcgac ccatcagttg caagacttgc tgggtcaacg
     4081 cttgttgctg ctgcaatatc tgttcaagaa gcgagctcgg tatgtttgcg gttcccaagt
     4141 ttcccgctgc agcagtttgc tgcgcaggtg cggtggcggc cacttgcgct gccggaggcg
     4201 caaaagcgtc agcaggtaac gtcgcatcga gatgctcggc gatttgttgt agcgaaccca
     4261 attcctccat caactgacgg aaagttatgt ttacgccgaa tttccggtga aaaacttgcg
     4321 ccgcttgcgt caacaaaagc gaatccagcc ctaattcaag caggctcgta gcggggtcaa
     4381 cctgcgacaa gtcgtacccg ctaagttctt cgagcaacgt acgcgaagca acaagcaagc
     4441 gatctttgcg cggaagcgcg ggttctgcgg gagttacggg ttgtggcgaa ggcgaggttt
     4501 gttgtgccgt ctctgtgttt ggtgtactcg cgagacttcc cgccatttgt gtaggagatt
     4561 gtgctaccga agctggtgca tgcgagacag cgatcggaga gtccggccaa taacgtttac
     4621 gttcgaacgg ataggtcgga agcgccgccc ggagccttcg ttcattggca taaaattgtt
     4681 gccaatctac gcta
//
LOCUS       DATCBB010000354         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_303738, whole genome shotgun sequence.
ACCESSION   DATCBB010000354 DATCBB010000000
VERSION     DATCBB010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303738"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VII37_03915"
     CDS             <1..238
                     /locus_tag="VII37_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068901.1"
                     /translation="IPIITILGLQFGTLLAGTIVTETIFSWPGIGRLTVQAISSRDYP
                     LLQGCILVIAVSYVVVNLLTDLLYTLIDPRVRLS"
     gene            235..1104
                     /locus_tag="VII37_03920"
     CDS             235..1104
                     /locus_tag="VII37_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068902.1"
                     /translation="MTFLASLRQFLRQSILARAGFIAAVILIVAALLAPWIVPADPTA
                     QNLPIRLSAPSHAHWMGTDELGRDILSRTIFGARISLLVAISVVCGCGSIGLIIGMLA
                     GYSGGWFDRFVNLLLINAFLSFPGILLAIAFAAFLGPGIGKVILALIITGWAGYARLA
                     RAQVLKAKELEFVLAARSLGASHRRILVRHLLPNILQPILIQATIGMAGAILAESTLS
                     FLGLGVLAPLPSWGSMLNDARSHLFDAPHMAVFPALAVMTAVLAFNLLGDAWRDWLDP
                     RTRSQMAALESSR"
     gene            complement(1174..1971)
                     /locus_tag="VII37_03925"
     CDS             complement(1174..1971)
                     /locus_tag="VII37_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY5068903.1"
                     /translation="MANPIVTLTTDYGTNDHLVGTLKGVLLKINPELTIVDITHHVNP
                     YDLLDGALTIGAAYAYFPPRTIHIVVVDPGVGTTRRPLLVTAQNQYFVAPDNGVLSLV
                     YEREQDNLVVRHATAEHYFLQPISKTFHGRDIFAPVAAWLSKGWQTAAMGEEIQDFQR
                     FTLPRPKPVDGGSQGVVLRIDAFGNVITNFRAEDLPESAKNGSGLNLKVGTHTISRLV
                     DTFASGAADEPIAYVGSSGYLEIGINKANAARKLTIARGAAVILPKS"
BASE COUNT          427 a          594 c          699 g          616 t
ORIGIN      
        1 gattccgatc atcactattc ttggtttgca attcggcacc ttgctggccg gaacgatcgt
       61 cacggaaacg attttttcgt ggccgggaat cgggcggctc acggtgcagg ccatttcctc
      121 gcgagattat cctttgctac aaggatgcat tctggtcatc gccgtgtctt acgtggtggt
      181 gaatttactg accgacttgc tctacacgtt gatcgaccca cgggtgcggc tctcttgacc
      241 ttcctggcat cgctgcggca atttctgcgt caaagcattt tggctcgagc tggatttatt
      301 gccgcagtta tcttgatagt cgcagctttg ctggctccat ggattgttcc tgctgatccg
      361 accgctcaga atctcccaat ccggctctcg gcgccctcgc acgcgcactg gatgggaacc
      421 gatgagcttg gccgcgacat tctttcgcga acaatttttg gcgcgcgcat ttctttgctg
      481 gtggcgattt ccgtggtgtg cggatgcggt tctatcgggc tgattatcgg aatgctggcc
      541 ggctattcgg gcgggtggtt cgatcgtttt gtgaatttgt tgctgatcaa tgcgttcctt
      601 tcttttccag gaatactgtt ggccatcgcg tttgcggctt tccttgggcc tgggattggc
      661 aaagttattt tggcgttgat tatcaccggg tgggctggtt acgcgcgcct ggcccgcgcg
      721 caagtcttga aagcgaaaga attggaattt gtgcttgcgg cgcgttcgct tggtgcttca
      781 caccggcgca ttttagtacg tcatctgctg ccaaatattc tgcagccgat tttgattcag
      841 gcgacgatcg gcatggccgg agcaattctc gcggagtcca cgttgagttt tctgggactt
      901 ggtgtgctgg cgcccctgcc cagctggggg tccatgctaa atgatgcgcg cagccatttg
      961 tttgacgccc cgcacatggc ggtttttccg gcgttggcgg tgatgacggc ggtgctggca
     1021 tttaatttgt tgggcgatgc ctggcgggat tggcttgatc cgcgcacacg ctcgcagatg
     1081 gccgcgctgg agagttcgcg ctgatttatt tttccgccgg gatatttggg aagcgagtgg
     1141 tctcggggtc ggatgaacaa cgaccggcac gaactaactt ttgggaagaa tcacggcggc
     1201 gccgcgagcg attgttaatt ttcgcgcggc attcgctttg ttgatgccga tctccaggta
     1261 accgctcgat cctacgtagg caatgggctc gtccgctgcg ccactagcga aggtgtccac
     1321 cagtcgcgaa attgtgtggg tgcctacctt taaattcaaa ccgctgccgt tcttcgctga
     1381 ttcgggcaaa tcttccgcgc gaaaattggt tattacgttt ccaaatgcgt cgatgcggag
     1441 gaccacgcct tgcgatccgc cgtccaccgg tttggggcgg ggaagcgtga agcgctgaaa
     1501 atcctgtatt tcttcgccca tggccgcggt ttgccagccc ttggacaacc aggctgccac
     1561 gggcgcaaaa atatcgcggc cgtgaaatgt tttgctgatc ggttgcaaga aataatgttc
     1621 tgcagtcgcg tggcgaacca ctaagttatc ttgctcccgt tcgtaaacca gggacagaac
     1681 gccattgtcg ggtgctacga aatattgatt ctgagccgtg acaagtaacg ggcgccgagt
     1741 cgtaccgact ccaggatcca cgacgacgat gtgaattgtc cttggcggaa agtacgcgta
     1801 tgccgcaccg atagtgagcg ctccgtcgag caggtcgtaa ggattcacgt ggtgagtgat
     1861 atcgacgata gtaagttctg ggttgatctt aagcagcacg cccttgagcg tgccgaccag
     1921 atgatcgttg gtgccgtagt cggtggtcag cgtaacgatt ggattagcca ctgcttctcc
     1981 cggttgtttg tagagctcac tggtgccagt tgcgcaaccg aagacaaagg gttgtgcgtg
     2041 tgagcccgtt attgacgcta tcgaagggtg cggctggcgt caagaaatca ggcgtgaact
     2101 gctgtgcaaa ctgcaacaga aggcttgagg gcgcttgagg ctgagggttc catatcggca
     2161 actttctgcc gtggtttatt tgggattcgg gtctacgatt ccgattttgt ccacgccgct
     2221 gcatgccaga tatatgcggc cgtccggcgt ggcggccatg tttcggacca cgccaccgcc
     2281 ggacggaata ttggcgcgag cgaacttctc ggacgacgga tcaaatttga taatcg
//
LOCUS       DATCBB010000355         1146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_76146, whole genome shotgun sequence.
ACCESSION   DATCBB010000355 DATCBB010000000
VERSION     DATCBB010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76146"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1146
                     /locus_tag="VII37_03930"
     CDS             <1..>1146
                     /locus_tag="VII37_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068904.1"
                     /translation="ERISAPHGDHHGLWIDRNNPNRMINGNDGGATITVDGGKNWSTQ
                     MNQPTAQFYHITADNDFPYRVYGTQQDNSSVGIATASDHGFISRSDWESVGGGESGYV
                     AVDPRDSNIVYAGSYFGDISRFDRRTHQVQNIQQWPLDPDGYNAAVQKYRYTWTMPII
                     FSPHDPNELYHSSQFLFRSTDGGHSWQTISPDLSRNDKTKQQDSGGPITRDQASIEFY
                     DLIFTVAESPKQKGVIWAGTDDGLIQITRDDGKSWTNVTPKGVPEWAMVSLIEASPFD
                     AGTAYAAVDAHKLDNLKPYIFKTSDFGKSWTLLTNGLPETSYVHAVREDPKRKGMLYA
                     GTETGIFVSFDDGAHWQTLQLNLPTTPVHDMIIHNDDLLVATHGRAF"
BASE COUNT          269 a          349 c          291 g          237 t
ORIGIN      
        1 tcgaacgcat ttccgcgccg cacggcgacc atcacggttt gtggatcgac cgtaacaatc
       61 cgaatcgtat gatcaacggc aacgatggcg gcgccaccat taccgtggac ggcggcaaga
      121 attggtccac gcaaatgaac cagcccaccg cgcagtttta tcacatcacc gcggacaacg
      181 attttccgta ccgcgtttac ggcacgcaac aggacaacag ctccgtgggc atcgccaccg
      241 ccagcgatca cggcttcatc agccgctccg attgggaatc cgtgggcggc ggagaaagcg
      301 gctacgttgc tgtcgatccg cgtgattcca acattgttta cgcaggatcc tatttcggag
      361 atatttcgcg ctttgaccgg cgcacgcacc aggtgcaaaa catccagcag tggccgctag
      421 atcccgacgg ctacaacgcc gcggtacaga aataccgcta cacctggacc atgccgatta
      481 ttttttcccc gcacgatccc aatgagctgt accactcttc gcaattttta tttcgttcta
      541 ccgatggcgg gcactcgtgg cagaccatca gcccggacct gagccgcaac gacaaaacca
      601 agcagcagga ttccggcggc cccatcacca gggaccaggc cagcattgag ttttacgatt
      661 taatttttac cgtcgcggaa tcgccgaaac aaaaaggcgt gatctgggcc ggcaccgacg
      721 acgggctgat tcaaattacc cgcgacgacg gcaagtcctg gaccaacgtc acgccaaagg
      781 gcgttccgga gtgggccatg gtcagcttga ttgaagcctc gccgttcgat gccggcaccg
      841 cttatgccgc tgtcgacgcg cacaagttgg ataatttaaa accatatatt tttaagacct
      901 cagattttgg caagtcctgg acgctgctca ccaacggatt accggagact tcttacgtgc
      961 atgcggtgcg cgaagacccc aagcgcaaag gcatgctcta tgctggcaca gagaccggca
     1021 tctttgtttc tttcgacgat ggcgcgcatt ggcaaacctt gcagctaaat cttcccacta
     1081 cgccagtgca cgacatgatt attcataacg acgatctgtt agtcgccaca cacggccgcg
     1141 cgttct
//
LOCUS       DATCBB010000356         1102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_401226, whole genome shotgun sequence.
ACCESSION   DATCBB010000356 DATCBB010000000
VERSION     DATCBB010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401226"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /gene="rpsI"
                     /locus_tag="VII37_03935"
     CDS             complement(<1..330)
                     /gene="rpsI"
                     /locus_tag="VII37_03935"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY5068905.1"
                     /translation="MALVQHLGTGRRKRAVARVILRPGEGKITVNGRTFEHYFPSDTA
                     RAIVKQPLLATETADKFDILINTAGGGPTGQAGAAKLGISRALLEFNIELRGKLKELG
                     FLTRDARE"
     gene            complement(355..783)
                     /gene="rplM"
                     /locus_tag="VII37_03940"
     CDS             complement(355..783)
                     /gene="rplM"
                     /locus_tag="VII37_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY5068906.1"
                     /translation="MKTVIPSGKDIPRNWHIIDAENLVLGRVASKAANILMGKHKPSY
                     TPYIDMGDHIIVINAAKVRLTGRKEEQKLYRRHSGYPGGLTETSARKVRETRPQRMVE
                     EAVQGMLPKNKLGKQMYRKLQVYAGPKHPHDAQKPVALAV"
BASE COUNT          214 a          318 c          317 g          253 t
ORIGIN      
        1 ctcgcgcgca tcgcgcgtga ggaagcccag ctctttcaac ttgccgcgca gctcgatatt
       61 gaactcgagc agcgcccgtg aaatccccag cttcgccgcg ccggcttgcc cggtggggcc
      121 gccgccggcg gtattgatca aaatgtcgaa cttgtccgca gtctcggtcg ccagcagcgg
      181 ctgtttgacg atcgcgcgcg ccgtgtcgct gggaaaataa tgttcgaagg tacggccgtt
      241 gacggtgatc ttgccctcgc ccggccgcaa aataactctc gcaaccgcgc gcttgcgccg
      301 gcccgttccc aaatgttgca ctaatgccac gtttatccga tctcctctag agcgctagac
      361 cgccagcgcc actggcttct gcgcgtcatg cgggtgcttg ggacccgcgt atacctgcag
      421 cttccggtac atctgtttac ccagcttgtt cttcggaagc atgccctgga ccgcctcctc
      481 gaccatccgc tgcggacggg tctcgcgcac cttgcgggcg ctggtttccg tgagtccgcc
      541 gggatacccg gagtgacggc gataaagttt ctgttcttct ttgcgccccg tgagacgcac
      601 cttcgccgca ttaatgacga tgatgtgatc gcccatgtca atgtacggcg tgtagctggg
      661 cttgtgcttg cccatcaaaa tgttagcggc cttagaagcc acgcgcccca gaaccaggtt
      721 ttccgcgtcg ataatgtgcc agttacgcgg aatatccttg cccgagggaa ttacggtttt
      781 catgtagaca gaccttctcc gtgctattcg ttcaggatac aagtctagcg aatgggggcg
      841 ggggctgtca aacggactga tgggtcagga gataaggaca tttaaaactg cccagcattt
      901 acccgccgaa agagatagca tggacgcagg atgaacgcgg ctatccggaa catcgtcttt
      961 ctggtgccac tcctgtccat tttgacgtgg gggcagggtg aagatacaaa aatacggtta
     1021 ttcggaagcg ttgtggatcc gttgggtggc gcaatcgtaa actccagagt tattttgtat
     1081 gagatcgggc gcgacgatcc aa
//
LOCUS       DATCBB010000357         1536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_141624, whole genome shotgun sequence.
ACCESSION   DATCBB010000357 DATCBB010000000
VERSION     DATCBB010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141624"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VII37_03945"
     CDS             <1..409
                     /locus_tag="VII37_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068907.1"
                     /translation="ALSDLPGSHEFVFKRLDSGPHLGNLGIVNVHPEPGEESIFYPSG
                     SAQPLTEDYAIVALVHGLNPARHLLILAGTTTMGTQAAVEYVCANNSAEELLRRLAVP
                     VGGEVKPFEALLRVKVTRGVPVEIELVAVRDRK"
     gene            complement(420..1475)
                     /locus_tag="VII37_03950"
     CDS             complement(420..1475)
                     /locus_tag="VII37_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068908.1"
                     /translation="MRISSILLPLCLLVGAAFGQEIEPSAAGHDKTAPEKFGAAETLA
                     AAHEKLEHGQAQEAIAMLEQLRTTAGESAAGLQHELGIAYYRTGKLIPAEQAFARAME
                     QDAHDIESVQMRGLSLYRLGRPAAAIPFLERVRQWTPNANADANYVLGLCYLNSQRFD
                     DARLAFARQFAVPPNSGAAYLLLGKMLMQANLPEAAVDAARKALELSPKLALAHFMLG
                     EFYLFKSDTDQALKEFEQEREINPTNSALYDRLGDLYTRAGQFQQAQEALAKAISLDT
                     SSTGPFIQMGKVLLRRNDPQTALLYLQHAEKMDPGNFMTHTLLGQAYRSLGREDDAKK
                     EIDAASKIHAATQLKLD"
BASE COUNT          307 a          397 c          458 g          374 t
ORIGIN      
        1 tgcgctctcc gaccttccgg gcagccatga attcgtcttt aagcgattgg actccgggcc
       61 tcacctaggt aatttgggaa tcgtgaatgt ccatcccgag ccgggcgagg aatcgatttt
      121 ttatcccagc ggatcggccc agccgcttac ggaagattat gccatcgtcg ctttggtgca
      181 tgggttaaat cccgcgcggc acctcttgat cctggcggga acgacgacca tgggcactca
      241 agcggccgta gagtatgttt gcgcgaataa ttcggcggaa gaattgctgc gccggctggc
      301 tgtgcctgta ggtggagaag ttaagccctt cgaagcgctc cttcgggtga aggtaacgcg
      361 gggggttccg gttgagattg aactggttgc ggtgcgcgac cggaagtaac acagttattt
      421 cagtccaact tcaactgcgt tgcagcatga attttggatg cggcgtcgat ctccttcttg
      481 gcatcatcct cccgtcccaa actgcggtaa gcctgcccca agagagtgtg ggtcatgaag
      541 ttgccgggat ccatcttttc ggcatgttgc aaatatagga gcgccgtttg aggatcattg
      601 cggcgcagaa gcacttttcc catctgaata aatgggcctg tgctcgaggt atcaagcgaa
      661 atggctttag cgagggcctc ctgtgcttgc tggaattgcc ccgctctggt gtataagtcc
      721 ccaagccggt cgtacagggc ggagttggta gggttgatct cgcgctcctg ctcaaattct
      781 ttcagggcct gatcggtgtc cgacttaaaa agataaaatt cacctagcat gaaatgagcc
      841 agtgccagct tgggagataa ttcgagcgcc ttcctggctg catcgacggc agcttcgggc
      901 aggtttgctt gcatcagcat cttgcccagt aataaataag ctgcgccgga gttgggcggg
      961 actgcaaact gcctggcgaa tgccaaccgg gcgtcgtcga aacgctgcga gttgagatag
     1021 cagagcccga gcacgtaatt ggcgtcagca tttgcattgg gtgtccactg cctgaccctt
     1081 tcgagaaacg ggatggccgc cgcggggcgc cctaaacgat agagtgacaa tccgcgcatt
     1141 tgtacggatt cgatgtcatg cgcatcttgt tccatggcgc gcgcgaacgc ttgttcggcg
     1201 gggattaatt ttcccgtgcg atagtaagcg attcccaatt cgtgctgcag gcctgctgcg
     1261 gattcgccgg cagtcgtccg taactgctcc agcatcgcta tcgcttcctg agcctggcca
     1321 tgttcaagtt tttcgtgggc ggccgctaga gtttctgccg cgccgaattt ttcgggggca
     1381 gtcttatcat gaccggctgc agaaggctca atttcctgcc cgaaggcggc gccgacgagc
     1441 agacaaagcg gtaggaggat tgaagaaatg cgcatgttgc gattttagtc atagcttcca
     1501 acagttgcat ttgttcgtgg ccgggccggg ttgcat
//
LOCUS       DATCBB010000358         1261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_174247, whole genome shotgun sequence.
ACCESSION   DATCBB010000358 DATCBB010000000
VERSION     DATCBB010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174247"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VII37_03955"
     CDS             <1..350
                     /locus_tag="VII37_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5068909.1"
                     /translation="TTRFRQMQHGDAVISTITYGELHYGAERSQQPTHALESLGRLVS
                     LLPVVPLPEEAAAVYGEIRAALEARGEMIGGNNLWIAAHAKSAGLTLVTNNEREFKRV
                     PGLKLQNWTSRRP"
     gene            470..790
                     /locus_tag="VII37_03960"
     CDS             470..790
                     /locus_tag="VII37_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068910.1"
                     /translation="MHRRVDANPVRFRDGKPLAGQWRQLSEHRGSVAEKHRSPSQKDS
                     GDFSNTYGRKVDVADREHEALCWLVRWRVFFMVCAELWGYRKGSEWNVSHYLFATLGA
                     SLVP"
     gene            complement(829..>1261)
                     /locus_tag="VII37_03965"
     CDS             complement(829..>1261)
                     /locus_tag="VII37_03965"
                     /inference="COORDINATES: protein motif:HMM:NF028495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY5068911.1"
                     /translation="PEQAGWDWMSLQLDDHSELMLFHIRRKGGSIDLFSAGTYVDAQG
                     HTLHLRESDFVLQPVGDTWKSPKSGASYSVHWKISIAKLGIELEARTPLPSQELAGDS
                     HVAPTYWEGAIVLTGKRFGAAIAGSGYLEMTGYDKDADAPI"
BASE COUNT          268 a          340 c          372 g          281 t
ORIGIN      
        1 tgaccacgcg attccgccaa atgcagcatg gtgacgccgt tatatcgacg attacctatg
       61 gggagctaca ttatggcgca gagcgaagtc agcaacccac acacgctctg gaatctctgg
      121 ggcgcctggt atctcttttg cctgtggttc cgttgccgga ggaagcggca gcagtctacg
      181 gcgagattcg cgcagcgttg gaggcccgcg gtgagatgat cggtggcaac aatctctgga
      241 ttgctgctca cgccaaatcc gcggggctca ctctcgtcac gaacaatgaa cgcgagttca
      301 agcgcgtccc cggacttaag ctccagaact ggaccagccg ccgtccttga tcgcgtgaaa
      361 tgagttgcag cggcaaagtc caacacttcc ccgagcgaga tttttgaagg agtacgattc
      421 actacagtgc tgtcccgagc cggaccgcgg gtccagctga gatgctttca tgcatcggag
      481 agttgacgcc aatccagtgc gatttagaga tgggaaacca ctggcaggtc aatggcgcca
      541 attatcagaa caccgcggaa gcgtcgctga gaaacatcga tcgccatcgc agaaagattc
      601 tggagatttt tcgaatactt acggccgcaa ggttgatgtc gcggaccgcg aacacgaggc
      661 attgtgttgg ttggtgcgct ggcgggtatt ttttatggtg tgtgcggagc tatggggcta
      721 tcgcaaaggc tcggaatgga atgtttcgca ttacctgttt gcgaccttag gcgcatcgct
      781 ggttccctag attgaatcga cggctcagag atggcttgcc ggccaactct agattggggc
      841 atcagcgtcc ttgtcgtagc cagtcatctc cagatagcct gaacccgcga tggcggctcc
      901 gaagcgcttt ccggtgagga cgatcgcacc ttcccaatac gttggcgcga cgtgggaatc
      961 gccagccagt tcctgcgaag gcagcggcgt cctggcttcc aattcgatgc cgagttttgc
     1021 tatggagatt ttccagtgca ctgaatagct cgctccggat tttggacttt tccaggtgtc
     1081 gcccaccggc tgcaaaacga aatcggattc gcgcagatgg agggtgtgcc cctgtgcgtc
     1141 gacataagtg ccagcggaga acagatcaat cgagccgccc ttgcggcgta tgtgaaacag
     1201 catcagttct gaatggtcgt cgagctgcag gctcatccag tcccaacccg cttgttccgg
     1261 g
//
LOCUS       DATCBB010000359         1862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_206349, whole genome shotgun sequence.
ACCESSION   DATCBB010000359 DATCBB010000000
VERSION     DATCBB010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206349"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VII37_03970"
     CDS             <1..432
                     /locus_tag="VII37_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5068912.1"
                     /translation="PMLYNRQSLPANEQYERASQALELVGLSDRADHHPNQLSGGQQQ
                     RVAIARGLVNRPSLLLADEPTGNLDSQTSIEIMGVFQKLNATGITIIMVTHELDVARY
                     TKRMVILRDGKILTDEVVANRLNAEQELGKLKQAQQAVQLA"
     gene            447..809
                     /locus_tag="VII37_03975"
     CDS             447..809
                     /locus_tag="VII37_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY5068913.1"
                     /translation="MKNEEKDLLPRTKMFARRIIRLYCSLSKTDTVAQVLGKQVLRSG
                     TSVGANYREANRARSKAEFISKTGDCLKEADETLYWLELLLDENIVPEKKLGALASES
                     SELVAIFITILKKARDHA"
     gene            907..>1862
                     /locus_tag="VII37_03980"
     CDS             907..>1862
                     /locus_tag="VII37_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5068914.1"
                     /translation="MRSLLTMLGIIIGVGAVIGSVSLTTGATKQVEDQVASLGESVIT
                     VFSGNFTSGAMRGGWGSAPTLTVADAIAISGISNVVAVSPEVRDRAQVLANGLNWNTQ
                     IIGESPDYPQIRNWNIAAGSMFSDPDVRSLAKSAVIGKTVADQLFPNENPVGQTIRIR
                     NLPFLIVGVLAPKGFNLFGQDQDDVVIVPYTSHMHRITTRIFVNSILVEAANDKVINQ
                     VQTDITDLLLTRHRSKEQDFTVRNQLELMQAVTATSRAMGFLLAGVAAVSLVVGGIGI
                     MNIMLVSVTERTREIGIRMAVGAHGRDILLQFLIEAITLSSLG"
BASE COUNT          416 a          542 c          532 g          372 t
ORIGIN      
        1 ccgatgcttt acaaccgtca gagcctcccg gcaaatgaac aatatgagcg cgcctcgcaa
       61 gcgctcgaac ttgttggctt gagcgatcgg gcggatcatc atccgaacca gctttccggc
      121 ggacagcaac agcgcgtcgc catcgcgcgc gggctggtga accggccgtc gttgctgctc
      181 gcggacgagc cgaccggaaa tttggacagc cagacgagca tcgaaatcat gggggttttc
      241 cagaagctca acgccaccgg catcaccatc atcatggtta cgcacgaact cgacgtggcc
      301 cgctacacca agcgcatggt gattttgcgc gacgggaaaa ttcttacgga tgaagtggtg
      361 gcaaaccggt tgaatgcgga acaggagctg ggcaaattga aacaggcgca gcaggcggtg
      421 caactggcct gaaggaagaa tgaagaatga aaaatgaaga aaaagatttg ctgccgcgaa
      481 ctaaaatgtt tgcccgccgg atcatccgtc tttattgcag cctttcaaaa acggatacgg
      541 ttgcccaagt gttgggcaaa caagtgttgc gttcaggaac atcggtcggg gcaaattacc
      601 gggaagccaa ccgcgcccgt tcaaaagctg aatttatttc caaaacgggt gactgcttaa
      661 aagaagcaga cgagactttg tattggctgg agctgttgct ggacgaaaac attgtcccgg
      721 agaaaaaatt gggcgcgttg gcatccgaat ccagcgaact ggttgccatt ttcatcacca
      781 ttctcaagaa agcacgggac cacgcatgag ccgattcttc attcttcatt cttcattctt
      841 catttcccca agatgagaat tttcgcctca ctccggattg ccggacgcgc actgcgccgc
      901 aataaaatgc gttcgctgct gaccatgctc ggcatcatca tcggcgtcgg cgcggtcatc
      961 ggctcggtga gtttgacgac cggcgcgacc aagcaagtcg aagaccaggt tgccagcctc
     1021 ggcgaaagcg tgatcacggt cttttccggt aatttcacca gcggcgcgat gcgcggcggc
     1081 tggggcagcg cgccgacgct tacggtggcg gatgccattg cgatcagtgg gatttccaat
     1141 gtcgtcgccg tcagtccgga agtgcgtgac cgcgcgcagg tgctggccaa cggtctgaac
     1201 tggaacaccc aaatcatcgg cgagtcgccc gattatccgc agatccgcaa ctggaacatc
     1261 gccgccggtt cgatgttttc cgacccggac gtgcgcagcc tggccaagtc cgccgtcatc
     1321 ggtaaaaccg tggcggacca gttgttcccc aacgaaaatc ccgtgggcca gaccatccgc
     1381 atccgcaacc tgccgtttct gatcgtgggc gtgctggcgc cgaaagggtt caacctgttc
     1441 ggccaggacc aggacgatgt ggtcatcgtt ccttacacca gccacatgca ccgcatcacc
     1501 acccgcatct tcgtgaattc catcctggtc gaggccgcca atgacaaggt catcaaccag
     1561 gtccagaccg acatcaccga cctgctgttg acgcggcatc gcagcaagga gcaggatttc
     1621 acggttcgca accagctcga actgatgcag gcggtcacgg ccacttcgcg ggcgatgggc
     1681 tttctgctcg cgggcgtcgc cgccgtctcg ctggtcgtgg gcggcatcgg catcatgaac
     1741 atcatgctcg tcagcgtcac cgaacgcacc cgcgagatcg gcattcgcat ggcggtcggc
     1801 gctcatggcc gggatattct gctgcaattt ctcatcgaag cgatcacgct gagctcgctg
     1861 gg
//
LOCUS       DATCBB010000360         1676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_433707, whole genome shotgun sequence.
ACCESSION   DATCBB010000360 DATCBB010000000
VERSION     DATCBB010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433707"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VII37_03985"
     CDS             complement(<1..832)
                     /locus_tag="VII37_03985"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEY5068915.1"
                     /translation="MRLFGAGKDRVCAVTAAASANAMVRQVREALRETSTVELRLDWL
                     RSDAERRRLLAWVNRQKFGKRVRLLATCRRIPAGGRFKSGIKEEMFWLMQAGEAGCVW
                     CDVEVETLRELPGKMVRGFGLPPKVLLSVHDFKHTPSLPRAMTVADHSGVAAVKMAAR
                     ANTIADSMRLLRLTRKSKNCVAVPMGEIGLPARILALREGSALAYAPVGEATAPGQVS
                     LREMLHLYRAHELTRGTRVFGVIGDPVGHSLSPLLHNIGFIARNVDAVYLPFLVHGLK
                     D"
     gene            1011..>1676
                     /locus_tag="VII37_03990"
     CDS             1011..>1676
                     /locus_tag="VII37_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068916.1"
                     /translation="MRAKLAGLTLFLFFAAATTSAQEGAQPGSATNSPDYAAVNCSGF
                     ISDNVPADIRVISGEQSNLKITFAHGDYVYISRGRDKGVQVGDRFSVVRPTKDPVDVQ
                     WFKWQHKLIKAMGQFYSDVGQLRVINVQPNVSIAEVGYSCGYMQRGDIVRPYVERPSP
                     PFKDATAFDHFAPVSGKRVAMIVAGLDNSQLHGKNSTVYVNLGSNQSVRVGDYFRIFR
                     YEGP"
BASE COUNT          371 a          542 c          382 g          381 t
ORIGIN      
        1 aatctttcag cccatgcacc aaaaaaggca gatacaccgc atcgacgttt cgcgcgataa
       61 atccgatgtt gtgtagcagc ggcgacaacg aatgcccaac cggatcacca atcactccaa
      121 atacccgcgt ccctcgcgtc aattcatgcg cgcgatataa gtgcagcatc tcgcgcagtg
      181 aaacctgccc cggcgcagtc gcctctccca ccggcgcata ggctaacgca ctgccttcgc
      241 gaagcgccag aatccgcgca ggcaatccaa tttctcccat cggcacagca acacaatttt
      301 tcgacttccg cgtcagtctc aagagtcgca tgctgtccgc aatggtgtta gcccttgcgg
      361 ccatcttcac cgcggcaact ccactgtgat ctgctacggt catcgcgcgt ggcaatgatg
      421 gcgtgtgctt aaagtcatgc accgacaaca gcaccttcgg cggcaatcca aatccgcgca
      481 ccattttgcc cggcaactcg cgcagcgttt ctacctcgac atcgcaccac acgcacccgg
      541 cttctccggc ttgcatcagc caaaacattt cttccttaat cccgctctta aatctgcctc
      601 ctgccggtat tcgacggcac gtagccaaca gcctcacgcg ctttccaaac ttttgtctat
      661 tcacccaagc cagcagcctg cgcctctccg catcgctccg caaccagtcc agccgcagtt
      721 ctacagtgga tgtttctcgc agtgcctccc gaacctgccg gaccatggcg ttggccgacg
      781 cggccgcagt cacggcgcaa accctgtctt tacccgcccc aaatagcctc ataaccgtcc
      841 cactcgcccc atgccattga aaacaaggct cgtagcagtg cgtatcctcg cagtatcaaa
      901 cttgtaaagc aactctggcc aatttgttga cgacactcat ggcccctgct actttcgagc
      961 tatcgggttc tagtaccacc acaagacgtg tgtctagaag gagcatcgcc atgcgcgcca
     1021 agttagcagg attgacctta ttcctatttt ttgctgccgc cactacttcc gcgcaggaag
     1081 gcgcccagcc aggttcagcc acaaattcgc ccgactatgc ggccgtgaat tgttccggct
     1141 tcatctccga taatgttccc gccgacattc gtgtcatttc aggcgagcag tccaacctca
     1201 aaattacctt cgctcacggc gattacgtgt acatcagccg cggccgcgac aaaggcgttc
     1261 aagtcggcga tcgtttttcc gtcgttcgcc ccactaaaga tcctgttgac gtgcaatggt
     1321 tcaaatggca acacaaatta attaaagcca tgggtcagtt ctattccgat gttgggcagc
     1381 tccgcgtcat caacgtacag cccaacgttt ccatcgccga ggttggttat tcttgtggct
     1441 atatgcagcg cggcgatatc gtgcgccctt acgtggaacg ccccagtcca ccctttaagg
     1501 atgcaaccgc tttcgaccat ttcgcgccag ttagcggtaa gcgtgtcgcc atgatcgttg
     1561 cgggattgga taacagccag ttgcacggca agaacagcac ggtctacgta aatcttggta
     1621 gcaatcaaag tgtgcgcgtg ggagattatt tccgcatttt ccgttatgaa ggccca
//
LOCUS       DATCBB010000361         2618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_174276, whole genome shotgun sequence.
ACCESSION   DATCBB010000361 DATCBB010000000
VERSION     DATCBB010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174276"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VII37_03995"
     CDS             complement(<1..962)
                     /locus_tag="VII37_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5068917.1"
                     /translation="MAVNLPIYMDNHATTPVDPRVLEAMLPYFTQKFGNAASRNHAFG
                     WAGEEAVENARQQIASLINATPKEIIFTSGATESDNLMIKGIAEMYREKGNHIITQSI
                     EHKAVLDTCKRLEKYGYEVTYLPVGKDGRVNPDDVRKAITPKTILITIMYANNEIGVI
                     NPIAEIGKIAKEHGIFFAVDGVQAVGKIPVDVQKDNIDLLAITGHKIYGPKGVGALYV
                     RRRNPRVQLSAIADGGGHERGMRSGTLNVPGIVGLGKACEICQQEMSKESERMRGLRD
                     RLKAGLEAKLDEVFVNGSLEHRLPNNLNMSFAYVEGESLLMGIN"
     gene            complement(975..1412)
                     /locus_tag="VII37_04000"
     CDS             complement(975..1412)
                     /locus_tag="VII37_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEY5068918.1"
                     /translation="MFKLSKKADYGLIAVKHLAMHRDQHACSATEIAEEYGISTMLMA
                     KVLQKLARQRIVTAKHGASGGYLLAKEPSQITALDVISAIDGPVLITSCVTSHGNCGA
                     ADKCSVREPLRRVNESILSVLSAVTISHMSEEQHEHVLVELQT"
     gene            complement(1628..>2618)
                     /locus_tag="VII37_04005"
     CDS             complement(1628..>2618)
                     /locus_tag="VII37_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5068919.1"
                     /translation="KWDIEPSAYVQDEMHFGNWNISAGLRFDHYAFVVHESAWSPRLG
                     VSRYLSSLNLLLHASYDRVFQTPAMENLLLASSPQLNSIDPVVLRLPVRPARGNYYEV
                     GATKSLASKLRIEANIFRRDFHNYPDDDTLLDTGVSFPIAFAHARIFGEELRLEVPEW
                     WRFSGYLSYSNQSGIGSGPITGGLFLGSDAPGALTDASQFAVSQDQRNTARARIRFQA
                     EKRVWVAMSAQYGSGLPANLSSTETPSFLLAQYGSAILDRVNFERERTRPTFALDVAA
                     GAQLYHKELRSVSLQIQASNVTDRVNVLNFASLFSGTAVSVPRSISGGLKLTF"
BASE COUNT          586 a          664 c          760 g          608 t
ORIGIN      
        1 tcgttgatgc ccatgagcaa agattcgccc tccacatagg caaaactcat attgagattg
       61 ttgggcaggc gatgctccag ggagccatta acgaagactt cgtcgagctt ggcttccagg
      121 ccggccttga ggcggtcacg caagccgcgc atgcgttcgg attccttcga catttcctgt
      181 tggcaaattt cgcaagcttt gccgaggccc acaatgcccg gaacattcaa tgtgccggaa
      241 cgcataccgc gttcgtggcc gccgccatcg gcgatagccg agagctgcac gcgcgggtta
      301 cggcgacgaa catacaacgc gcctacaccc ttgggtccat aaattttgtg gccggtgatg
      361 gccagaagat cgatgttgtc cttctgcacg tccacgggaa ttttgccgac tgcctggacg
      421 ccatctaccg cgaagaagat gccgtgttcc ttggcaatct tgccgatttc agcgatgggg
      481 ttgatcacgc caatttcgtt attggcgtac atgatggtga tgagaatcgt tttcggcgtg
      541 atggctttac gcacatcatc gggattgacc cggccgtctt ttccaaccgg cagataggtt
      601 acttcgtagc cgtatttttc taggcgctta caagtgtcca ggacggcttt gtgctcgatc
      661 gactgggtga tgatgtgatt gcccttttcg cggtacattt ccgcgatgcc cttgatcatc
      721 aagttgtcgg attcggttgc gccgctggtg aaaatgattt ctttcggcgt ggcgttgatg
      781 agcgaagcga tttgctggcg cgcattttca acggcttctt cgccggccca tccgaaggcg
      841 tggttgcgac ttgcggcgtt gccaaatttt tgcgtgaagt acggcagcat cgcttcgagt
      901 acgcgagggt cgaccggggt ggtcgcgtga ttgtccatgt aaatcggtaa attcacagcc
      961 ataatcctgt tctcctaagt ttgcaattcc accagcacat gctcgtgctg ttcttcgctc
     1021 atgtgagaaa tcgtaacggc gctaagtacg ctcaaaatgc tttcattgac gcggcgcaaa
     1081 ggctcgcgca cggaacactt gtcggcggcg ccgcaatttc cgtgactggt gacgcacgaa
     1141 gtaatcaata ccgggccatc gatggctgaa atcacgtcca gcgcggtaat ttgcgagggc
     1201 tccttggcca gtagatagcc gccgctggca ccgtgtttgg cggtgacgat gcgctggcgg
     1261 gccagcttct gaagcacctt ggccatgagc atcgtggaga tgccatattc ttcagcgatc
     1321 tcggtggcgc tgcaggcatg ctgatcccgg tgcatagcga gatgcttcac cgcaatgagg
     1381 ccgtaatccg cttttttaga cagcttgaac atataacgtt tgatgttgaa agcgacttga
     1441 agttgatatg agactaattc ggtcgcccac ctaagcgctc atatcagacc gattcagtcg
     1501 caaatgaagt ttagtgctga ttggcggggt tgtcaatcat ggatttcggt accttgcgag
     1561 caaggccagt gttttcagct aattaagtcg aggaaattat ctgaaaaatg gcgattcgcc
     1621 tgtgaaatta aaaggtcagc ttcaacccgc ccgaaatgct tcgcggtaca gagacagccg
     1681 tgcctgaaaa gaggctagcg aaattcagaa cgttgacacg atcggtgacg ttcgaagcct
     1741 ggatctgcaa cgatacgcta cgcaattctt tgtgatacag ctgtgcgccg gcggcgacgt
     1801 cgagtgcgaa agttgggcgc gtacgctcgc gctcaaaatt gacgcgatca aggattgcgg
     1861 agccgtactg cgcgagcaga aaactcgggg tctcagtaga gctgagattg gcaggaaggc
     1921 cgctgccgta ctgggcgctc atagctaccc acacgcgctt ctcagcttgg aacctgatgc
     1981 gggcccgcgc ggtattgcgc tggtcctgcg agacggcgaa ttgactcgcg tctgtaagtg
     2041 cgcccggggc gtcgctgcct aaaaacaaac cgccggtgat ggggccgctg ccgataccgg
     2101 attgattcga atagctaagg taaccagaga agcgccacca ttcgggaact tccagacgca
     2161 attcttcgcc gaaaatgcgg gcgtgggcaa acgcgatcgg aaagctgacg ccagtgtcca
     2221 gcagagtgtc gtcgtcagga taattgtgga agtcgcggcg aaaaatattt gcttcgatac
     2281 gcagtttact ggcgagagat ttggtggcgc ctacttcgta ataatttcca cgcgctgggc
     2341 ggaccggtag ccgtaaaacg acgggatcaa tggaattcaa ttgaggagag ctggcgagca
     2401 gcaaattttc catggcgggc gtttgaaaaa cgcgatcgta ggaagcgtgc agcagcaaat
     2461 tcaatgagga tagataacgg gaaacgccga ggcgcgggct ccaggccgat tcgtgcacga
     2521 cgaatgcgta atgatcgaag cgcaagcccg cggaaatgtt ccagttgcca aagtgcattt
     2581 catcctgcac gtaagcagag ggctcaatat cccacttg
//
LOCUS       DATCBB010000362         1096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_206388, whole genome shotgun sequence.
ACCESSION   DATCBB010000362 DATCBB010000000
VERSION     DATCBB010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206388"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..504
                     /gene="rpsT"
                     /locus_tag="VII37_04010"
     CDS             241..504
                     /gene="rpsT"
                     /locus_tag="VII37_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY5068920.1"
                     /translation="MANHFSALKRARQTEKRSARNRANTSRLRTTLRDLRESLVKGEK
                     KSAEQVFRETVSGLDKAVQKGVLHKNTASRYKSRLNARLNALK"
     gene            complement(570..989)
                     /locus_tag="VII37_04015"
     CDS             complement(570..989)
                     /locus_tag="VII37_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068921.1"
                     /translation="MRPNDMPWVRIALAALAGGVASSMTDWLFMGDWLYKRYDRHPEI
                     WRYRGGQGETKAIVWASLLPFLTCAAFALLCAGLHLHSYSATLKLALAIWLIGPLPLT
                     IANALFIKLTPAVAAFHTLGWLFKLAIAGLAVTVILG"
BASE COUNT          285 a          316 c          285 g          210 t
ORIGIN      
        1 ccaaactttg ccgctgtgcg cctaggacgt ccaatatggg cactccaaaa attctcaaag
       61 acgttacgat ctgcctgcgt tcgaaccgca cggggaagca cgtacatgtg gcggagccct
      121 ggctgccaat tgacccttgc caggtactta cgtaaaatag cttatcgaac gccgctagag
      181 ctgctctttc tgacggcctc taatttgcaa aaatccaaaa taaaaaggta cgaagtttat
      241 atggcaaatc atttttccgc gctaaagcgc gcccggcaga ccgaaaaacg cagcgcccgt
      301 aaccgcgcca acacttcccg cctgcgcacc acgttgcgcg atttgcgcga gagcctcgta
      361 aaaggcgaaa agaaatccgc cgagcaggtt tttcgggaaa ccgtatccgg tttggataaa
      421 gccgtgcaaa aaggcgtcct tcacaagaac accgcatcgc ggtacaagtc ccgcctaaat
      481 gcgcgcctga atgcattgaa gtaaatttca gacagccgtc cgcccgaatc agccgccatt
      541 tacccttctg ccacaaagtt cgcgaaatcc tacccgagaa tcaccgtcac cgccagcccg
      601 gcaatggcca gtttgaagag ccaccctagc gtgtgaaatg cggcaacggc aggagtcagt
      661 ttgatgaaga gggcattcgc aatcgtcaac ggcaatggtc caatgagcca gatagccaga
      721 gccaacttga gagtcgcgct gtatgagtgc aggtgcaaac cggcacagag caaggcaaac
      781 gcagcacatg tgagaaaggg cagcaggctg gcccagacga tagctttcgt ttctccctgc
      841 ccgccgcggt aacgccaaat ctccggatga cgatcgtagc gtttgtacag ccaatcaccc
      901 ataaataacc agtcggtcat cgatgaagcg acgccgcctg ccagcgccgc gagcgcaatg
      961 cgaacccaag gcatatcgtt gggacgcact ctaacatgtc aggccgcctg gcgtgaagcc
     1021 attttccagc ggggaatcaa cgcgcttctt gtatggagca ttgcatactg acgggggtgg
     1081 gtgtacgtta tcggtc
//
LOCUS       DATCBB010000363         1136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_174285, whole genome shotgun sequence.
ACCESSION   DATCBB010000363 DATCBB010000000
VERSION     DATCBB010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174285"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VII37_04020"
     CDS             complement(<1..416)
                     /locus_tag="VII37_04020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5068922.1"
                     /translation="MFIPLKDLNPHRRFPIINVLLICANIAVFLYEITLPPHLFKAFL
                     MANATIPARFPAFLAGNIDFVGAFLPLVTSMFLHAGIAHIAGNMLFLWIFGDNVEDFF
                     GHIGYLFFYLACGIGSGLLHVLFNFGSSLPALGASGA"
     gene            686..>1136
                     /gene="recO"
                     /locus_tag="VII37_04025"
     CDS             686..>1136
                     /gene="recO"
                     /locus_tag="VII37_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY5068923.1"
                     /translation="MPARETEAIILKTFPLGEADRLVSFLGRATGRMRGVAAGARRIK
                     NRYGSTLQLLSHVQVWYVEKETRDLVRIQQCEPLESFNKSQSDYFLSTGMAVVSEVAE
                     IALPEREVSEPMFRLILLTAQVIERTGEWSLPLSYFAFWTVRLGGWLP"
BASE COUNT          303 a          272 c          321 g          240 t
ORIGIN      
        1 gcgccgctgg cccccagtgc cggcagcgaa gaaccaaaat taaacagtac atgaagcagg
       61 ccagaaccga tgccgcaggc taagtagaaa aatagataac caatgtggcc gaagaaatct
      121 tccacattgt cgccaaaaat ccaaaggaaa agcatgttgc cggcaatgtg ggcgataccc
      181 gcgtgcagaa acatgctggt taccagcggg agaaacgcgc cgacgaaatc aatattcccg
      241 gctagaaaag ccgggaatcg tgcgggaatt gtggcgttag ccatcaggaa agctttgaac
      301 aaatgcggtg gcaatgtgat ttcgtacaga aataccgcaa tgttcgcgca gatgagcaga
      361 acattgatga tagggaagcg cctgtgcggg ttaagatctt tgagtggaat aaacacgaat
      421 agccgcggtc agagtaacca aaaccgacaa aaagataaag cagcttggga aacactcgtg
      481 aaccgcatga accctcaatc gtcttacgca agaagtaaat aattttttct aatttgcgat
      541 aaacgtgaaa ttcctcgtca atttcagctt ttctacgcgc acacattgcg cgcgcgttgc
      601 gggtgtgata attaaacatg ttcgcgtgct gcaaagttcc ggcctatacc aggctcggtg
      661 aaggtggcgg cgcgccaaaa acttcatgcc cgcccgggaa acggaagcca ttattttaaa
      721 aacctttccg ctgggcgagg ccgaccggct ggtgagtttt ctcgggcgcg ctacaggcag
      781 gatgcgtggg gttgcggccg gcgcgcggcg catcaagaac cgctacggct ctacgctaca
      841 gctgctgtcg cacgtgcaag tttggtatgt cgaaaaggaa acgcgcgatc tagtgcgcat
      901 tcagcaatgt gaaccgctcg agtcattcaa caagtcgcaa agcgattatt ttcttagcac
      961 cgggatggca gtggtaagtg aagtggcgga aatcgcgcta ccggagcgcg aagtatccga
     1021 gcccatgttt cgtttgatac tgcttaccgc gcaggtgatc gagcgaaccg gtgaatggtc
     1081 tttgccgctg agctattttg cgttttggac agtgcgtctg ggtggctggc tgcctc
//
LOCUS       DATCBB010000364         1446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_271677, whole genome shotgun sequence.
ACCESSION   DATCBB010000364 DATCBB010000000
VERSION     DATCBB010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271677"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VII37_04030"
     CDS             complement(<1..216)
                     /locus_tag="VII37_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068924.1"
                     /translation="MSETASASAAIETPPTERALHGLFSWVATVDHKRIGILYLLTTL
                     VFFVIGGVEALLMRIQLARPNNTFLSPD"
     gene            complement(213..1148)
                     /gene="coxB"
                     /locus_tag="VII37_04035"
     CDS             complement(213..1148)
                     /gene="coxB"
                     /locus_tag="VII37_04035"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5068925.1"
                     /translation="MSTSFSPIFDTASSQAAAISGLFKVVLVICGVILVIVTSMVGVS
                     LIRFRQRTGEGEPRPYFGNRKLEIIWTVGPTLIIIWLFVLTARGMRQSDPPANRQPDL
                     IVIGHQWWWEVRYPQTGVVTANEIHIPVGQKWLVRLESADVIHDFWVPALARKIQMIP
                     GLTNHIWLEADAPGKYDGTCAEYCGAEHSWMRFSVIAESSSSFDAWLRGQENPAAIPA
                     IDSVQSGLKIFQTMTCVNCHSIRGVSIAANAAPDLTHLADRTILGAGVLSNTETNLFR
                     WLKNPQATKSGCFMPNLKLTDAQAGALTSYLETLK"
     gene            complement(1172..>1446)
                     /locus_tag="VII37_04040"
     CDS             complement(1172..>1446)
                     /locus_tag="VII37_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068926.1"
                     /translation="DAYETLLWDVMKKDATLFMRADQVEAAWELLMPVLEAWQATAPS
                     DFPNYAAGTWGPEDAQRLLAPGHLWPLPTELVDQEKTRINHKHQKQ"
BASE COUNT          330 a          399 c          386 g          331 t
ORIGIN      
        1 atctggcgac agaaacgtat tattgggccg cgcaagttga atgcgcatga gcaacgcctc
       61 cacgccgcct atgacaaaaa aaacgagtgt ggtcagcaga tagagaatgc cgatgcgttt
      121 gtgatccacc gtcgccaccc agctaaaaag cccatggagt gccctttccg tcggaggcgt
      181 ttctatcgca gcggaagctg aagcggtttc gctcatttga gagtctccaa ataactcgtc
      241 agcgcgcctg cctgcgcgtc cgttaatttc agattgggca tgaagcagcc cgatttagtt
      301 gcctgcggat ttttcagcca gcggaacaga tttgtttctg tgttggataa tacgcctgcg
      361 cccaaaattg ttcggtcagc caaatgtgtt aaatctggcg cggcgttggc cgcaatggaa
      421 actccacgaa tggaatgaca attgacgcag gtcatcgtct ggaaaatctt caaaccgctc
      481 tgaactgaat ctatggctgg aatggccgca ggattttcct gtccgcgtag ccatgcgtcg
      541 aacgacgacg acgattcggc gatgacagag aaacgcatcc aagagtgttc cgcgccgcaa
      601 tactccgcac aggtgccgtc gtattttccc ggcgcgtcgg cttccagcca gatgtgattc
      661 gtcaggccgg gaatcatctg tattttgcgc gccagggccg gcacccagaa atcgtggatc
      721 acgtccgccg attccagacg gacgagccat ttctggccga ccggaatgtg aatttcattt
      781 gcggtcacca cgccggtttg cggatagcgc acttcccacc accattgatg gccgatcacg
      841 atgaggtcag gctgccgatt cgctggcgga tcagactgtc gcatcccgcg cgccgtaagg
      901 acgaagagcc agatgatgat cagtgtcggt ccgacggtcc aaataatctc caacttccgg
      961 ttcccgaaat acggtcgcgg ttctccctcg cctgtccgtt gccgaaagcg aatcaaactg
     1021 actccaacca tgctggtgac aatcaccaga attactccac aaattaccag cacgactttg
     1081 aacaggccag aaatcgcggc agcttgagaa gaagcagtat cgaaaatggg actgaagctg
     1141 gttgacattt cttttgagga gaaatcttcg tttattgttt ttgatgttta tgattgatcc
     1201 tcgttttctc ctgatccacc agttcagtcg gcagcggcca aagatgtccg ggagcgagca
     1261 gccgttgggc gtcttccggc ccccaagttc cggcggcgta attgggaaaa tcgctcggtg
     1321 ccgtggcctg ccacgcttca agcaccggca tcaggagttc ccaagcagcc tccacctgat
     1381 cggcgcgcat aaaaagcgtc gcgtcttttt tcatcacgtc ccacagaagt gtttcgtagg
     1441 cgtcgg
//
LOCUS       DATCBB010000365         5474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_109792, whole genome shotgun sequence.
ACCESSION   DATCBB010000365 DATCBB010000000
VERSION     DATCBB010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109792"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VII37_04045"
     CDS             complement(<1..1116)
                     /locus_tag="VII37_04045"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5068927.1"
                     /translation="MQFTPALLLRRTTSTILGILFLALVPLAHGKDLKTLDDRRKELK
                     QLIADEWEYEMRESPEAATAIGDYRYNDRWSDNSLAHIQQQKQDINKWLARFTAIDTA
                     GFPEQEKLNKSLMVRNLKEELEGMELKTYEMPVNQFFGAHLQLAQFVALVPFDSTKHY
                     EDYLARLHKVPQLVNELIEILQQGEKDKLLPPRFLLEKTVTQCKSIAGPEGEANAFGQ
                     PVAKIPEAVPLEDRKRLHDAIITAVNTEVRPAYNKLADFLAKEYAPKGRTEPGVWALP
                     NGDALYRFAVRQSTTTNMDPEAIHQLGLKEVARIEAEQLAIAKKLGFADLKTFRASLK
                     TNPKLIPTSRDQLVEAYKGYIAQMEPELPKLFGLLPKT"
     gene            1301..2638
                     /locus_tag="VII37_04050"
     CDS             1301..2638
                     /locus_tag="VII37_04050"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEY5068928.1"
                     /translation="METERIALSQRERDRLRMLHEVKRKQLTQSEAARRLKISDRQIR
                     RLLVGLQKRGDRAVIHGLRGRPSNRRYVVRFEQQILTRVRQRYADFGPTLAAEHLAQE
                     GLAVSRETLRKWMVKASLWRPRTQRVKTIHVWRERRASFGELVMQDSSPFRWLEERGP
                     ACQLIAVIDDATSRFYARFTEHDTTEENLRTFGEWLRRYGRPLAHYTDKNSIFRTSAL
                     APLGEQLRGQKARSHFGRALRELGIEWIAAHSPQAKGRIERLFETLQDRLVKEMRLAG
                     IDNLAAANHFLEMHFLPAWEQRFTVAPRKPRNAHRRLDPEQRLEEILSVRVGRQVADD
                     HSVSWDGTRWGVKREEVCAGLRGAQVEVERRLDGSHWLRFRGRYLRLRHCPAPLRPSA
                     SPSGLRPPGLAERRAKPKDKIKPKYHVPAEHPWRKPWNRTFLSCEKPDISTLR"
     gene            2720..4048
                     /locus_tag="VII37_04055"
     CDS             2720..4048
                     /locus_tag="VII37_04055"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY5068929.1"
                     /translation="MRAVDLIRKKRDSGEHPREEIEFLISGYTRGEIPDYQMAAWLMS
                     AWIRGLSRGETTALTHAMLHSGEVLDLSSLPGKKVDKHSTGGVGDKTSLILAPIVAAG
                     GLIVPMLSGRGLGHTGGTLDKLESIPGFNTNLSLKEFRQVLGECGMALIGQTAEIAPA
                     DKKIYALRDATSTVENKGLICASIMSKKLAEGLDALVLDVKTGSGAFMKREEDAVGLA
                     ELMVDAGQNMGKKMVALITDMDQPLGRAAGHANEIVECIEVLNGKGPADLRELSVELS
                     AWMFYLGEKTKSVAEGRKLAERMISSGEAREKFRQCIRLQGGDERIINEPELLPKAVS
                     DIDVKSSVSGTLVATNCEQFGIALAMLGGGRETKEDRIDHAVGLEFHKKIGERVEKGE
                     PLAAIHYNSDAKLTEAKSIVMESFVIDQDSGGQTKPPRPPKPLIRRIIGS"
     gene            4179..5411
                     /locus_tag="VII37_04060"
     CDS             4179..5411
                     /locus_tag="VII37_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY5068930.1"
                     /translation="MGRFAGILGLAAMLGLAYIFSTDRKAIRLKTVLWGFGLQLCFAI
                     FVLRFEAGRRIFQFLGYYVNLLLSYAFVGSQFVFGELGMKDSPEHFIFAFQVLPTIIF
                     IAAFFALLYHIGVMQVLIRGAAWVMTRIMGASGAESLNVAASIFMGQTEAPLTIRPFL
                     NRLTRSELMTVMTSGMAHVSGGIMAAYIAFGIKAEHLLAAVIMTAPGTLLMAKMLVPE
                     TEVPLTAGCVEMPEMEKEANFLGAISRGTVDGLHLALNVGAMLISFLAMLALVNAIMG
                     GAHNWLAAHHVGWFPSSLQQVFGWIFAPVAWLIGVPWRDCATIGNLLGTRMVINEVVA
                     FQQLGTMQGALDPRSFTIATFALCGFANFSSVGIQIGGIGALAPDQRGNLARLGIRAM
                     LAGTMANLMSASFVGVLT"
BASE COUNT         1162 a         1302 c         1731 g         1279 t
ORIGIN      
        1 cgtctttggc agcaacccaa agagcttagg taactcgggt tccatttggg cgatgtaacc
       61 cttgtatgct tcgactaatt gatcgcggga cgtcggaatc aacttgggat tcgttttgag
      121 tgaagcgcga aaagtcttta gatctgcgaa tccgagtttt ttggcgatgg ctagttgttc
      181 ggcttctatg cgagccactt ccttcaaccc caactggtgg atggcctcgg gatccatgtt
      241 ggtggtggtg gactgacgga ctgcgaagcg atacaaggcg tcgccgtttg gcagcgccca
      301 gacaccgggt tctgttcgac ccttgggcgc gtactctttt gccagaaagt ccgcgagttt
      361 gttataagcg ggacgcacct cggtgtttac cgcggtgatg atggcgtcgt gaaggcgctt
      421 gcggtcctcc agcggcacgg cttcaggaat ttttgccact ggttgtccaa aggcgttggc
      481 ttctccttcc ggaccggcaa tggatttgca ttgggttacg gttttctcaa gcaggaatcg
      541 cggaggcagt aatttgtctt tttcaccttg ttgcaggatc tcgatgagtt cgtttacgag
      601 ctgaggaacc ttgtgcaagc gcgcgaggta atcttcataa tgttttgtcg aatcgaaagg
      661 cactagagcg acgaactgtg caagttggag atgcgcccca aagaactggt tgactggcat
      721 ttcgtaggtt ttcaactcca tgccttcgag ttcttccttg aggttgcgga ccatcaggga
      781 cttatttagt ttttcctgct cggggaagcc ggcggtatcg atggcagtga agcgagccag
      841 ccatttgtta atgtcctgtt tttgttgctg aatatgcgcg agggagttat cgctccagcg
      901 atcgttgtag cggtagtcgc ctatcgcggt ggccgcttcg ggactttcgc gcatctcgta
      961 ttcccattca tcggcgatga gttgcttcag ttctttgcgg cggtcatcca gcgtcttgag
     1021 gtccttccca tgggcgaggg gaactagcgc gaggaagagg attcccaaga ttgttgaggt
     1081 ggttcgtcgc agcaaaagcg ccggtgtgaa ttgcatttaa gctcctcaga gtgaagaaag
     1141 acgacaatgt ctcgaaattc ggatcatcgt gtcgcgcaca gcatcttacg tacgtatttg
     1201 tcagcagtcg agtctgcaaa atgagtctgt cgctgcaaaa tgagaatgtc ctatttgagc
     1261 aaagtagaaa tgtccgcttt cataggtggc cgagggccgc atggagacgg agcgaattgc
     1321 attgagccaa cgagagcgcg accggctgcg catgctacat gaggtcaagc gtaagcaact
     1381 cacgcaaagc gaagccgcca gacggctaaa gatcagcgat cgacagattc gacggttgtt
     1441 ggtgggactg cagaagcgcg gtgaccgcgc ggtgatccat ggattacgcg gacggccatc
     1501 gaaccggaga tacgtcgtac gtttcgagca gcagattttg acgcgggtgc gccaacgcta
     1561 cgcggacttt gggccgacgc tggccgccga gcacttggcc caggaggggc tggcggtgag
     1621 ccgcgagacg ctgcgcaagt ggatggtgaa ggccagcttg tggcgccctc gcacacaacg
     1681 cgtaaagacc atccatgtgt ggcgggagcg ccgcgccagt tttggcgagc tggtcatgca
     1741 ggatagctcc ccgtttcgtt ggttggagga gcgcgggccg gcttgccagt tgattgcggt
     1801 gatcgacgac gctaccagcc gtttctatgc gcgttttaca gaacacgaca ctacggaaga
     1861 gaacttgcgg actttcgggg aatggttgcg gcgttacgga cgtcctttgg cgcattacac
     1921 cgataaaaat agcatcttcc ggacgtctgc tctggcgccg ctgggagaac aattgcgggg
     1981 acaaaaagcc cgttcgcatt tcggacgagc gctccgcgag ttggggatcg aatggatcgc
     2041 ggcgcacagt ccgcaggcca aagggcggat cgaacgactg ttcgaaacgc tgcaggaccg
     2101 cttggtaaag gaaatgcgcc tggccgggat cgacaacctc gctgccgcga atcatttttt
     2161 ggagatgcac tttcttccgg cgtgggaaca gcgtttcacg gtggcacctc gcaagccgcg
     2221 caatgcccat cgacggttgg atccagaaca gcggttggag gaaattctaa gcgtgcgcgt
     2281 gggtcgccag gtagccgacg atcacagcgt aagctgggat ggcacccgct ggggcgtgaa
     2341 gcgcgaagaa gtgtgcgcgg gtcttcgcgg agcgcaggtg gaagtcgaac ggcgtctgga
     2401 tggctcgcac tggctacgct tccgcggccg ctatctccgc ctgcgccatt gcccagcacc
     2461 cctgcgaccg tccgcaagtc cttccggcct acggcctcca ggacttgcgg aacgaagagc
     2521 aaaaccaaaa gacaaaatca aacccaaata ccatgtgcct gctgagcacc cctggaggaa
     2581 accgtggaac cggacatttc tatcttgcga aaaaccggac atttctactt tgcgttgaca
     2641 gtctttgtaa aaaccgcgta gcggggcgcg ttgaccttcg aatggccgga tgttacgctc
     2701 ccgcgtctca tccaactgca tgcgcgctgt cgacctgatc cgaaaaaaac gcgactccgg
     2761 tgaacatccc cgcgaagaaa tcgaattctt gatttcgggg tatacacgcg gggaaattcc
     2821 cgattaccag atggccgcgt ggctaatgtc ggcatggatt cgcggcctta gccgcggtga
     2881 gacgaccgcg cttacgcacg cgatgctgca ttccggagaa gtgctggatc tttcgagcct
     2941 gccgggtaaa aaagtcgata aacattccac cggtggagtg ggtgacaaga cgtctttaat
     3001 actggcgccg attgtggcag cgggtgggct cattgtgccc atgcttagcg gacggggcct
     3061 tgggcatacc ggcgggacgt tggacaaact ggaatccatt cctgggttca acaccaattt
     3121 gtcgttaaag gaatttcgcc aggtgctggg cgagtgcggc atggcattga ttgggcagac
     3181 ggcggaaatc gcaccggcgg ataaaaagat ttatgctttg cgcgacgcta cttcgacggt
     3241 cgaaaacaaa gggctgattt gcgcttccat catgagcaag aaacttgcgg aaggattgga
     3301 cgcactggtc ctggacgtta aaactggttc aggcgcgttt atgaagcggg aagaagatgc
     3361 cgtgggcctt gcggaactga tggttgacgc tggacagaac atgggcaaga aaatggtggc
     3421 cttgattacc gatatggacc aaccattggg gcgcgcggcc ggacacgcta acgaaattgt
     3481 ggaatgcatc gaggtgctga acggaaaggg acctgccgac ctacgggaac ttagcgtgga
     3541 gctttcagcg tggatgtttt atttggggga gaagacgaag agcgttgcgg aaggccgaaa
     3601 acttgccgaa cgcatgattt ccagcggcga agctagagag aaattcaggc agtgcattcg
     3661 gctgcagggc ggagacgagc gcattattaa cgagcctgaa ttattgccca aggcagtttc
     3721 cgatatagat gtgaaaagca gcgtcagcgg aacgctggtg gcgacgaact gcgaacagtt
     3781 tggcatcgcg ttggccatgt taggcggggg ccgggaaact aaagaggacc ggatcgatca
     3841 tgccgtggga ctcgaattcc ataagaagat cggtgagcgc gtagaaaaag gcgaacctct
     3901 ggcggcaatt cattacaata gcgacgccaa gttgacggag gccaagagca tcgtgatgga
     3961 gagcttcgtg atcgatcaag acagcggcgg gcaaacgaaa cctccgagac ctccaaagcc
     4021 gttgatccgg cgaatcattg gttcgtagtc gggttcgtag tcggtttcgt aaagtcgggg
     4081 ataagctcga cgcttgatcg ccgcttgact agaagcgttt tcgacgcgat cttagaaatt
     4141 cgcttgtcga tcgcagcgcg ggaaatgagt gtataaggat gggacgattt gcaggaattt
     4201 tagggctggc tgccatgctg gggctagcct atattttttc caccgatcga aaagccattc
     4261 gcttgaaaac cgtgttgtgg ggctttggct tgcaactatg ttttgccatt tttgttttgc
     4321 gctttgaagc ggggcgcagg atttttcagt ttcttgggta ttacgtcaat ctgctattga
     4381 gttacgcctt cgtcggttcg caatttgttt ttggcgagct ggggatgaaa gattcgccgg
     4441 aacattttat ttttgccttc caggttttac ctaccattat ttttatcgcg gcgttttttg
     4501 ctttgctgta tcacatcggg gtgatgcagg tgctgattcg cggagcggcg tgggtcatga
     4561 cgcggattat gggtgctagc ggcgctgaat cgttgaatgt cgcggcgagt atttttatgg
     4621 gacaaacgga ggcgccgcta acgattcggc catttctgaa ccggctgacc aggtcggaat
     4681 taatgacggt gatgactagc ggcatggcac atgtgtcggg cgggatcatg gccgcgtata
     4741 tcgcgtttgg aattaaagcg gagcatctgc tggcggcggt aatcatgact gcgccaggga
     4801 ctttgttgat ggccaagatg ctggtgccgg agacggaagt gccgctgacg gcggggtgcg
     4861 tggaaatgcc ggaaatggag aaggaagcga attttctggg cgcgatttcg cgcgggacag
     4921 tggatgggtt gcatctggca ctgaatgttg gagccatgtt gatttccttt ctggcgatgc
     4981 tagcgttggt gaacgccatc atgggcggcg cgcacaattg gcttgccgcg caccatgtgg
     5041 gctggtttcc atccagcttg cagcaagttt ttggctggat ttttgctccg gtagcttggc
     5101 tgattggcgt tccgtggcgg gattgcgcga cgatcggaaa tttattgggc acgcgcatgg
     5161 tcattaacga ggtggtggct ttccagcagc ttggcaccat gcagggcgcg ctcgatccgc
     5221 ggtcgtttac gattgcaacg tttgcgttgt gcgggttcgc gaatttcagt tcggtgggaa
     5281 ttcagatcgg cggaatcggg gccctggcgc cggatcagcg cggcaatctt gcgaggctgg
     5341 gtattcgcgc gatgctggcg ggcaccatgg cgaacctgat gtcggcttcc tttgttggag
     5401 ttcttacatg atgcagggcg aggaaagttt ggcggagggg cgcgcgtcac atggagcggc
     5461 gcaaaaatct gctt
//
LOCUS       DATCBB010000366         3215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_238286, whole genome shotgun sequence.
ACCESSION   DATCBB010000366 DATCBB010000000
VERSION     DATCBB010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238286"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..549)
                     /locus_tag="VII37_04065"
     CDS             complement(232..549)
                     /locus_tag="VII37_04065"
                     /inference="COORDINATES: protein motif:HMM:NF039544.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HEY5068931.1"
                     /translation="MPQPSPFSAWLIFFPIAAVGTFSLLLLGLGRTGQRPPDSAWKGS
                     FYSNPDDPALLVPKRFGIGYTLYFGNPWSWAVLAFVLLMVAVPFILFLLASSTQHLPA
                     AAR"
     gene            717..989
                     /locus_tag="VII37_04070"
     CDS             717..989
                     /locus_tag="VII37_04070"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY5068932.1"
                     /translation="MPKFVIEREMPNVGNLSDSEFCAAAQKSIQALRDIGPQIQWLHS
                     YVTGNKIYCVYLAPDEATVQKHASKAGIPANRISAVRRMIDPTTAE"
     gene            complement(1058..>3215)
                     /locus_tag="VII37_04075"
     CDS             complement(1058..>3215)
                     /locus_tag="VII37_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5068933.1"
                     /translation="EGEVAAVKELLLRDDVRVVTLTGPGGIGKTRLGLQVANEVREWF
                     DGGICFIPLAAVSDASLIPSIIAQGCGIRETGRPVSIQSLKEYLQDLRSNLLLFFDNF
                     EHMLAAASVVAELITAAQKLRVLVTSRAPLHIYGEYEFPVPTLELPDLRSVTSREILA
                     TNPTIALFMARAVAVKPNFELTEENARAVATICTRLDGLPLAIELAAARIKLLSPSAM
                     QSRLESSLQLLTGGAKDLPMRQQTLRRTMDWSYDLLSLAEQTLFRRLSVFVGGCTLEG
                     VEAVCNTKQDLEIDVLDGMGSLVNNSLVRQIEQSAGEPRFTLLDTVREYGLERLAASG
                     EEAAIKRAHAAYCLVLAEECAVQAADPAQRAWVSLLEVEHNNCRAALEWLTRSGNAEW
                     GLRLGAALFQFWETRERLTEGRDRLEKLLKLEGAKNHSNSRVRVLFAAGVLATEQGDH
                     QAAHKLLEQSLQIARELDDTRGVGIALNALAANAREDGQISKAKSLFEESLAVWRDLN
                     DRAMVARALSNVASVVKSQRNYELARALHEESRVIFEELGDSTGIAWSLNYQGDVAQE
                     QGENSVARELYEQSLNIFRKLGDKWGVAGCLVDLGNLAREQGDEKKSRLHYAESMKLF
                     QELGQKRGVARVLDCLACSAALQSQPERALRLAGSAAAMRRVLGAPLPVAEQTRLEKT
                     LDPARQSISPAKAAAAWMDGWTMATDKAIQEALSFG"
BASE COUNT          711 a          978 c          834 g          692 t
ORIGIN      
        1 cccacattgg ggcgccccac aatcacaagc gatggtaaat gttgtgccat gaataatttt
       61 gtgccttgaa gctttgcgct ataaaaaggg gtcgcgggcc acttgtgttg aatttgtcgc
      121 gcgcacaagc atcgcgcaag ccgcagcagg acgtgccaca actgtaccgc atcgtcgcgt
      181 gagtccaaaa ccagcgatgc gcagctcaaa ccgaaaatcc agtgtggagg actaccgggc
      241 tgcggcaggc aaatgctgtg tgctggacgc taacagaaac aaaataaatg gtaccgcgac
      301 catgagtagc acgaacgcca gcaccgccca gctccaggga tttccaaaat ataatgtgta
      361 tccgatgccg aagcgcttcg gcaccaacag cgccggatca tctgggttgg agtaaaacga
      421 tcccttccac gccgaatcag gcggccgctg accggtgcgg cccaatccca acagcagcaa
      481 actaaatgtt ccgaccgccg cgatcgggaa aaagatgagc caagcagaaa agggagaagg
      541 ctggggcatg gggttcctcc gccgcggcta ttgtagtcgc aaaaattaaa tttgctagcg
      601 cggactgagt tttttcccag gaatactcga ttcactcgat tgactgaaaa ttacatcgag
      661 gataaagttt ggctcatcga caaatcaatt ttagaagccg cgaggtggag tgaactatgc
      721 ccaagtttgt tatcgagaga gaaatgccaa atgtgggcaa tctgtcggac agcgaatttt
      781 gtgccgccgc tcagaagtcg atacaggctc tgagggacat tggtccccag attcaatggc
      841 tgcacagcta tgtgaccggg aacaaaattt actgcgttta tttagccccg gatgaagcta
      901 ctgtccagaa acatgcgagc aaggccggga ttcccgccaa ccgcatctcc gcggtgcgca
      961 ggatgattga tcccaccacg gcggagtaat ttcgttcgcc gcgtattccg tacagcacgc
     1021 cggagtgctt gaacgatcta tgataatcct gagccggtca tccaaacgat aaagcttctt
     1081 gtatggcttt gtccgttgcc atcgtccaac cgtccatcca tgcggctgcc gctttggccg
     1141 gggagatgga ttgcctcgcc gggtccaaag ttttttccaa tcgcgtttgc tccgccaccg
     1201 gcagcggcgc gcctagaact cggcgcatag ccgcagccga ccccgccaat cgcaacgcgc
     1261 gttcgggctg agactgcagt gccgcggaac aagccaggca gtcaagcacg cgcgctaccc
     1321 cgcgtttctg ccccaattcc tgaaaaagtt tcatgctttc cgcgtagtgc aaacgtgatt
     1381 ttttttcatc accttgctcg cgtgccaggt ttcccaagtc caccaggcat cccgcgacac
     1441 cccatttgtc tcccagcttc ctgaatatat tcaggctctg ctcgtacagc tcccgcgcca
     1501 ccgagttttc tccctgctcc tgggccacat cgccctgata attgagagac cacgcgatgc
     1561 ccgtgctatc gcccagttcc tcaaaaatca cgcgagactc ttcatgtaaa gcgcgcgcca
     1621 gctcgtagtt ccgctgcgac ttgacaacgc tggcgacatt gctgagcgcc cgcgccacca
     1681 tcgcacgatc atttaaatca cgccagaccg ccaggctctc ttcgaacagg gattttgcct
     1741 tcgatatctg gccatcttcg cgagcgtttg cggccagggc gttcaaggcg atacctacac
     1801 cccgtgtgtc gtctaactcg cgagcgattt gcaggctctg ttccaataac ttgtgtgcgg
     1861 cctggtgatc gccctgctcg gtagccagca cgccggcagc gaatagcacc cgcacgcgag
     1921 aattggaatg gtttttggcc ccttcaagtt tgagaagttt ctccagccgg tcgcgcccct
     1981 ccgtcaaacg ctcgcgcgtc tcccagaatt ggaagagcgc cgctcccagg cgcagtcccc
     2041 actccgcgtt tccgctgcgc gtcaaccatt cgagcgctgc acggcaattg ttgtgttcca
     2101 cttccaacaa actcacccat gctcgctgtg cgggatcggc ggcttgcact gcgcattctt
     2161 cggccagcac caggcagtac gccgcatgtg cacgttttat cgcggcttcc tcgccactcg
     2221 ccgcgagccg ttccagccca tactcgcgaa ccgtgtccag taacgtaaaa cgcggctcgc
     2281 ccgcggattg ctcaatctga cgaaccaggc tgttgttgac cagcgatccc atgccgtcca
     2341 gcacatcgat ttcaagatcc tgcttggtat tacagactgc ctcaacgccc tcgagagtgc
     2401 acccgccaac aaaaacggag agtctgcgga agagagtctg ctctgccaga ctgagcaggt
     2461 catagctcca gtccatcgtt cttcgcaacg tctgctgtcg catcggcaga tctttcgctc
     2521 cgccggtcag aagttgcagc gaactttcca gtcgcgactg catcgcagac ggcgacagta
     2581 acttgatgcg tgccgcggct agctcgatgg ccagaggcaa gccatccagc cgggtacaaa
     2641 tggtggccac ggctcgcgca ttttcctcgg tgagttcaaa atttggctta acggcgacgg
     2701 cgcgtgccat gaataacgcg atggtgggat tcgtcgcaag gatttcacgc gatgtcacgg
     2761 agcgcaggtc cggcaactct agggtgggca ccgggaattc gtattcgcca taaatatgca
     2821 aaggcgcgcg gctggtcacc aggacgcgca atttctgagc cgcggtaatt aattccgcca
     2881 ccaccgacgc cgctgccagc atgtgctcga aattgtcaaa gaacagtaat aggttcgacc
     2941 ttaagtcttg taaatattcc ttcaggcttt ggatgcttac tggccggccg gtttcccgta
     3001 ttccacatcc ttgagcgatg atcgacggaa ttagcgatgc atcacttacc gcagccagtg
     3061 gaataaaaca tattccgccg tcgaaccatt cgcgtacctc attcgcaacc tgcagcccca
     3121 gccgcgtttt tccgatgccg ccaggtccag tcagcgtgac cacccggaca tcgtcgcgca
     3181 acagcaattc cttcacggcc gccacttctc cttcg
//
LOCUS       DATCBB010000367         6216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_12201, whole genome shotgun sequence.
ACCESSION   DATCBB010000367 DATCBB010000000
VERSION     DATCBB010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12201"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1029)
                     /locus_tag="VII37_04080"
     CDS             complement(139..1029)
                     /locus_tag="VII37_04080"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY5068934.1"
                     /translation="MSIDNIKTVAVLGAGTMGNGIAHAFARVGYKVILRDVEQRFVDR
                     GMDTIGQNLGREVKKGKITDAERVSTMERLLPVTDIANIAAADFLVEAVPEKIEIKRA
                     VLGDADRLLRDDVIIASNTSSISMTTLASLTRRPERFVGMHFMNPVPLMILVEVIRAL
                     QTSDATFQTTMQLAEKLGKKPVAVNDAPGFVSNRVLMPLINEAAYCVMEGVATAEAVD
                     AVMKTGMNHPMGPLELADFIGLDVCVDIMHVLQEGLGDPKYRACPLLKKYVAAGWMGR
                     KSGRGFYVYGEAGQSKTASK"
     gene            complement(1032..1559)
                     /locus_tag="VII37_04085"
     CDS             complement(1032..1559)
                     /locus_tag="VII37_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068935.1"
                     /translation="MSDFGNEGSFVILWAGENPSVHVNLLEKLEANGIAFNDKSLGDD
                     EVAPTSDPLPIDWKPRFGFEVTVLSTDYPAAKELLENLLQREPENLEIPAQPEVVDPA
                     TMSPKANEAPTTLQVWKGTDNKLAQFLTSALSENNLVARVANEAGSTAIYVAEGNQAR
                     ALEIVREVTQGTPPK"
     gene            1878..2459
                     /locus_tag="VII37_04090"
     CDS             1878..2459
                     /locus_tag="VII37_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5068936.1"
                     /translation="MKDENKALAQGLQRRDPELLDRLIEQYQFRLFRYLVYITNDRTR
                     AEDFFQETWIRVLERGHQYDGKSRFEGWLFAIARNLVIDWQRQRKVQSLDALTDSEQA
                     HPLQIKDERSASALQAVLNSEQDAQVQGALQQIPAIYREVLLLRFQEELQIDEIAGVL
                     GTPPSTVKSRLYRGLEALRGTLEKGVALEGGVS"
     gene            2456..2956
                     /locus_tag="VII37_04095"
     CDS             2456..2956
                     /locus_tag="VII37_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068937.1"
                     /translation="MSENIHENIHARAQQLISQERVEGISASENTWLAAHLQGCDSCA
                     IFAQKTSTALRSLQTIPIALPAGLAERTQFRVGLRAQQLRHSEPRRRALWIAAGISWV
                     AGVASAPLVWRLFAWLGEQTGVPKLVWEMGFGLWWAVPALIAAVVLLSEHEKQSGERY
                     WLQRSQ"
     gene            3149..3799
                     /locus_tag="VII37_04100"
     CDS             3149..3799
                     /locus_tag="VII37_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5068938.1"
                     /translation="MSDLQANSTFSEKMRRIRFRRTKENTRFRDELRIIPKWLIVMVI
                     ALFVIAQVAFLIANLGAHALSSPEGQVWPFPNNPPLSAVSVAGIVTAASIPLASLLLG
                     FGYVNRDAKRRGMNSALWTILVVVLSQSLALGFLIYFIMREPLPYNCPQCGGTVGARF
                     NFCPNCKCNLQPTCPQCKREVGEMDKFCPYCACELTPATAAVSSSLNDSPIVRSSV"
     gene            complement(3836..5020)
                     /locus_tag="VII37_04105"
     CDS             complement(3836..5020)
                     /locus_tag="VII37_04105"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5068939.1"
                     /translation="MEIPVILSAVRTPVGKFQGGLSSFSAVELGAKAIAEAVRRAELD
                     PKQIDEAILGNVVQAGLGQNPARQAALKGGCDPRVAAMTINKVCGSGLKAVALAAQGV
                     QLGESEIVVAGGMESMSNCPYLLPQARTGYRIGDGKLIDSMIHDGLWDAYEDFHMGMT
                     GELVAEKYGITREEQDRFAFESHQKAVRARKSCFFEAQILPIEVPQKKGPAIVIKYDE
                     SPREDTSMEALAKLRPAFKKDGTVTAGNAPGTNDGAAALVVTSERHAQSLNKKPMARI
                     VAQAVSGVEPKWVMMAPVEAVEKLLTKTGWDRDKDVDLVELNEAFAVAAIAVTRQLRL
                     NPEKVNVNGGAVAIGHPIGASGARILVTLLYELQRRSLKRGIAALCLGGGNAVALAIE
                     MM"
     gene            complement(5066..5560)
                     /locus_tag="VII37_04110"
     CDS             complement(5066..5560)
                     /locus_tag="VII37_04110"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5068940.1"
                     /translation="MPISELLVTEWDQEGANTRKAIERVPAEKWDWKPHPKSGTLGWM
                     ASHIATLPHFAVTTIKTSEFDIEGGERPKVDGHSRLLPVFDEQLKKGHDAISSITDEQ
                     LREIWTLKWKGQVLFAMPKYNVIRGMCFNHIIHHRAQLTMYLRSLDIPVPALYGPSAD
                     EQKF"
     gene            complement(5989..>6216)
                     /locus_tag="VII37_04115"
     CDS             complement(5989..>6216)
                     /locus_tag="VII37_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068941.1"
                     /translation="DGNGWLFQEITTRLPGRIDPTTTTFASANDLASAFRRAAAAHGE
                     HEKRIGAADPNWPDWYAKYMVAEQAGTELPT"
BASE COUNT         1307 a         1658 c         1777 g         1474 t
ORIGIN      
        1 cgactacagg ctgtattctc cggccgacac tacggccgac agttcaagcg cgcgcttaat
       61 tcaaaaattg caaaccaaat ccggaaataa tacgagatag ctcataaacc gctcaatcgt
      121 aaagtgacgc accactcact attttgaggc agttttggat tggcctgctt ctccataaac
      181 gtaaaacccg cggccgctct tgcgccccat ccaaccggcc gccacatatt tctttaacaa
      241 cggacacgcg cggtatttgg gatcgcccaa gccttcttgc aatacgtgca taatgtccac
      301 gcacacatcc agcccgataa aatccgctag ctccagcggc cccatggggt ggttcatgcc
      361 cgttttcatt accgcatcca cggcttccgc ggtggccacc ccttccatca cgcaataggc
      421 agcttcgttg atgagcggca tcagcacccg gttggacacg aaacccggtg cgtcatttac
      481 cgccaccggt ttctttccta atttttccgc aagttgcatg gtggtttgga acgtcgcatc
      541 gctggtttgc agcgcgcgaa ttacttccac cagaatcatt aacggcacgg gattcatgaa
      601 atgcatcccc acgaaccgct cgggccgccg cgttaacgat gccagcgtgg tcattgatat
      661 agaggaggta ttgctggcga tgatgacatc gtcgcgtaag aggcggtctg catcgcccag
      721 aacagcgcgt ttgatttcga ttttttccgg aacagcttcc actagaaaat ccgcggcggc
      781 aatattggcg atatccgtga ccggcagcaa ccgttccatg gtgctgaccc gttccgcgtc
      841 ggtgattttg cccttcttta cttcgcggcc cagattctgg ccaatggtat ccatgccgcg
      901 atcaacgaag cgctgttcga cgtcgcgcaa aattactttg tagcccacac gcgcaaacgc
      961 atgcgctatg ccgttaccca tcgtgcccgc gcccaacacc gcgacagtct tgatgttgtc
     1021 tatgctcatg cctacttcgg aggcgtgcct tgcgtcactt cacgcacaat ttccagggcg
     1081 cgtgcctgat tcccttccgc cacataaatt gcggtggatc ccgcttcgtt ggcgactctg
     1141 gccaccagat tattctcact tagcgcactg gtcaggaact gcgcgagctt gttgtcggtt
     1201 cccttccaca cttgcagtgt agtcggcgcc tcatttgctt taggcgacat agtcgctggg
     1261 tcaaccactt ctggctgcgc gggaatctct agattttccg gctcgcgctg caacagattt
     1321 tcaaggagtt ccttggccgc gggataatcc gtggacaaca ccgtaacttc aaatccaaag
     1381 cgcggcttcc aatctatggg caagggatca gaagtaggcg ccacttcgtc gtcgcccaag
     1441 gatttatcgt tgaaggcgat gccgttggcc tccagctttt cgagcagatt gacatgcacc
     1501 gaaggatttt ctccggccca tagaattaca aaagaacctt cgttgccaaa atcagacatg
     1561 acgcctcgct gtaagtggtt gcatttcgct acgcgggtgc tgtgctagga cgcgaaaaaa
     1621 ccgggccagt ctgtcgcgct cgacgcgaac atcgccaggt gagtttacta tacaacccgc
     1681 ctatcccgca ggggtctcag ttgcacttgg ggtgcctgta aatccaacga accaaaggcc
     1741 taatagcctg ctgagtcttt tctgctaact ggcgcgtagt tctctgtaca atgagcgcga
     1801 cgtttttatt tccggcaatc caaacccgaa gtgcgtggcc tacgcggtcg cttccgacct
     1861 gtgcgttctc tgttttgatg aaagacgaaa ataaagcact ggcgcagggc ctgcagcggc
     1921 gcgacccgga gttgctggac cggctcatcg agcaatatca gttccggctg tttcgttacc
     1981 tggtgtacat cactaatgat cgtacgcggg ccgaagattt tttccaggaa acgtggattc
     2041 gtgtgctgga gcgtggacat cagtacgacg gaaaatcgcg ctttgaaggt tggctgttcg
     2101 ccatcgcacg caatttggtg atcgattggc agcgccaacg aaaagtgcag agcctggacg
     2161 cgctcacgga ttcagaacag gcgcatcctc tacaaataaa agacgagcgc tctgcatccg
     2221 cgctgcaagc agttttgaat tcagagcagg atgcacaagt acagggagcg ctgcagcaaa
     2281 ttcccgcgat ttaccgcgaa gttttgttgc tgcgttttca ggaagaattg caaatcgacg
     2341 aaatcgcggg agtgctgggc acaccgccat ccacggtgaa gtcacgattg tatcgcggac
     2401 tggaagcttt gcgcggcacg cttgagaagg gcgtggcact cgaaggagga gtttcatgag
     2461 cgaaaatatt catgaaaata tccatgcgcg cgcgcagcaa ctgatttcgc aggaacgcgt
     2521 cgaaggcatc tctgcatcgg aaaatacctg gcttgctgcg catttgcagg ggtgcgacag
     2581 ctgcgcaatt ttcgcgcaga aaacttccac ggctttgcgt tcgttgcaaa ccataccgat
     2641 tgccttgcct gcaggactgg cggagcgcac gcagtttcgc gtgggtttgc gcgcgcaaca
     2701 acttcgccac agcgaaccgc gccggcgcgc actgtggatt gcggcgggca tttcgtgggt
     2761 cgcaggtgtg gccagcgcgc ctcttgtgtg gcggctattt gcctggctcg gagaacagac
     2821 cggcgttccg aagctggtgt gggaaatggg ttttggattg tggtgggcag tgcccgcgtt
     2881 aatcgctgcg gtcgtgctgc taagcgagca cgagaaacaa agcggcgagc ggtattggct
     2941 gcaacgcagc caataagtga gagcgtatcg gaatatccgt gagtgtgaga gcagcagcgg
     3001 atgcggtgga attgagcgcg tcggaagatg cgcgatgtga acgcggcggg gcgcggcgag
     3061 agcaagtgca gcggatgtgc ggtgagtgta agagcgaaca ttttgattac gcgagttaga
     3121 tttttttgaa gttaatgagg gcatagcaat gtcggatctt caagctaaca gcacgttcag
     3181 tgaaaagatg aggcggattc gttttcgccg gaccaaagag aacacgcgat ttcgcgatga
     3241 gttgcggatc attccgaaat ggctgatcgt catggtcatc gcgcttttcg taatcgcgca
     3301 ggtggcgttt ctcatcgcca acttaggcgc gcatgcgctt agctcgccgg aaggccaagt
     3361 gtggcccttc cccaataacc ctccgctttc cgcggtcagc gttgctggca tcgtcaccgc
     3421 cgccagcatc ccgctagcat ctttactgct ggggttcggt tacgtaaatc gtgacgccaa
     3481 acgccggggg atgaactcgg cgctgtggac aattcttgta gtggtgttat cgcagtcgct
     3541 ggcgctcggg ttcctcatct atttcataat gcgcgaaccg ttgccatata actgtccgca
     3601 gtgcggcggg acggtcggcg cgcgatttaa tttttgcccc aactgcaaat gcaatctcca
     3661 acctacttgt ccgcaatgca aacgagaagt tggtgagatg gataaattct gcccgtattg
     3721 cgcttgcgaa ttgacgccag cgactgccgc cgttagttca tccctgaatg attcgccgat
     3781 tgtccgcagt tccgtgtaat tcggtatcac tcctccgcga tcgttttagt ttcggctaca
     3841 tcatttctat cgcgagggct acggcgttgc ctccgcccag gcagagggcg gcgattccac
     3901 gctttaggct gcggcgctgc aattcgtaaa gcagagtgac gaggatgcgc gcgcctgagg
     3961 caccgatggg atggcctatg gctacggcgc cgccgtttac gtttactttt tcggggttga
     4021 ggcgcaattg gcgcgtcacg gcgatggcag cgacagcgaa agcttcgttg agttcgacta
     4081 gatcgacgtc cttgtcgcgg tcccagccgg tcttggttag caatttttcg actgcttcaa
     4141 cgggagccat catgacccac ttgggttcga ctccgctgac ggcttgagcg acgatgcggg
     4201 ccatgggttt tttgtttagc gattgggcgt ggcgttcgct ggtgacaacc aatgctgcgg
     4261 caccgtcgtt tgtgccgggg gcgttgcctg cggtgactgt tccatctttc ttgaatgcgg
     4321 gtcttaattt tgcaagagcc tccatggagg tgtcttcgcg tggagactcg tcatatttaa
     4381 ttacgatggc ggggcctttt ttttgcggga cttcgatggg aaggatttgc gcttcaaaga
     4441 agcaagattt gcgcgcgcgc acggctttct ggtgactttc aaaggcgaat cgatcttgtt
     4501 cttcgcgggt gatgccgtat ttttctgcga ctagttcgcc ggtcatgccc atgtggaagt
     4561 cttcgtaggc gtcccacaag ccgtcgtgaa tcatggagtc gatcagtttg ccatcgccga
     4621 tgcgatagcc ggtgcgggct tgcggaagta agtaggggca attggacatg gactccatgc
     4681 cgccagcgac cacgatttcc gattcgccga gttgcacgcc ttgagctgct agtgcgaccg
     4741 ctttcaggcc ggatccgcac actttgttta tggtcatggc ggctacgcgc ggatcacaac
     4801 caccttttaa tgcggcctgg cgagcgggat tttgaccgag gccggcctgg acgacgttgc
     4861 cgagaatggc ttcgtcgatt tgttttgggt cgagctctgc gcggcgcacg gcttcggcaa
     4921 ttgctttggc gcccaattcg acggccgaaa aacttgagag gccgccctga aatttcccta
     4981 ccggggtgcg aacagcactg agaattactg ggatttccat tttgaatctc cgacttctga
     5041 acaactaacg gaccagcaat aacttttaga atttttgctc gtcggcgctg gggccgtata
     5101 gcgcgggaac gggaatatcc aaactgcgca agtacatggt tagctgcgcg cgatggtgaa
     5161 tgatgtgatt gaagcacatc ccgcggatca cgttgtactt cggcatggcg aacaggactt
     5221 ggcccttcca tttgagggtc cagatttcgc gcaattgctc atcggtaatg ctggaaatgg
     5281 cgtcgtggcc tttcttgagc tgctcatcga atacggggag cagccgagaa tggccgtcga
     5341 ctttgggacg ttcgccgcct tcgatatcga attcggaggt tttgatggtg gtaaccgcaa
     5401 aatgcgggag ggtggcgatg tgcgaggcca tccagccaag cgttcccgat ttgggatgtg
     5461 gtttccaatc ccacttctcg gctggcacac gctcgatagc cttgcgggtg ttggcgcctt
     5521 cctggtccca ttcggtgact agcagttcac tgataggcat tttttagctc cggacatagg
     5581 tgagtgggga ttgagggtcg attgtactgg aacacgagaa agacaggcaa acttgggctc
     5641 gtggggcaga aaaaaagatg tgccaccccc ccatgccccc tatttttgcc aaaagagctc
     5701 agaagacaat gataacaatt gagataagtg agctctagag gcaaatttgg attttggacg
     5761 gacgtaacgg ccttggacga cgtcgttttg aagaatagaa cagaacgagc cgcagaacac
     5821 gcaactgtac ctttaaaact gatccggtag gggatagcga caagcacgag atattttact
     5881 acatagatat atgatagaca actgataaag ctgatgtgtc ggctggcgta acgctcgacg
     5941 cggacccaag ctcagcacca tccttcgtcc aggcagtcgc gcactagctc acgtcgggag
     6001 ctccgttccg gcctgctcag ccaccatgta cttggcatac cagtcgggcc agttcggatc
     6061 cgccgcgccg atgcgcttct catgctcacc gtgagcggcc gccgcacggc gaaacgcgct
     6121 cgccagatcg ttggcggacg cgaaggtcgt ggttgtgggg tcgatgcgac cgggcagccg
     6181 cgtggtgatt tcctggaaca accagccgtt gccgtc
//
LOCUS       DATCBB010000368         1887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_434013, whole genome shotgun sequence.
ACCESSION   DATCBB010000368 DATCBB010000000
VERSION     DATCBB010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434013"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VII37_04120"
     CDS             complement(<1..1364)
                     /locus_tag="VII37_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068942.1"
                     /translation="MASAFMKLFRILASLLFIAAGATAADKGKDNDQSNSLELTRPVR
                     TWEFLSAVGTRAGIFGNESGRMEAWVYPLKIFRDFHLRFHTEDRALPAESLARTVIVR
                     PESTTIVYTSDTFSVRETFFVPVDEPGAIITFEVETEFPLEIEAVFRRDFQLEWPAAL
                     GATYVNWEPERNAFYFSEEQKKFAAFLGSPTAAERRREYQTNYSSESDNSFRLGVTEK
                     GSDTKVIVLAASVQGRPQAEAAYQHLTTEYVKLLRQSTDYYRDYLSKTVSVELPDEEL
                     QRAYDWSRVSMIQGLVTNPYLGTGLVAGYRTSGESQRPGFAWFFGRDSMWTSLALNAS
                     GDFATTRKALEFIGKYQREDGKIPHEIAQGANFVPWFKDYPYAYASADATPLYIITMN
                     DYIVASGDTAFAKLKWDSVWKAYQFMRSTYDPQGLPQNLGFGHGWIEGGPLLPVKTEL
                     YQSG"
     gene            1631..>1887
                     /locus_tag="VII37_04125"
     CDS             1631..>1887
                     /locus_tag="VII37_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068943.1"
                     /translation="MPQGAAAAAASIAGTPLGLSFLNVAKEAGLNAKTIFGGEHSNKY
                     LLETTGCGVAFYDYDNDGWLDIFLVNGWRLEGFPAGQEPTS"
BASE COUNT          406 a          445 c          581 g          455 t
ORIGIN      
        1 aagccacttt ggtacagttc agtctttacg gggagcagcg ggccgccttc gatccagccg
       61 tgtccgaagc caagattctg cggcaaacct tgcgggtcat aggtggaacg catgaattga
      121 taggccttcc aaacgctgtc ccatttcagt ttggcaaagg cagtgtcgcc gctggccacg
      181 atgtaatcgt tcatggtgat gatgtagagc ggggtggcgt cggcggaagc gtatgcgtag
      241 ggataatctt tgaaccaagg aacgaagttc gcgccctggg cgatttcgtg aggaattttt
      301 ccgtcttcgc gttggtattt gccgatgaac tcaagggcct tgcgtgtggt tgcgaaatca
      361 ccggaggcgt tcagggccag cgaggtccac atggagtctc ggccaaaaaa ccatgcgaat
      421 cctggtcgct ggctttcgcc ggaggtgcgg tatccggcca cgagtccagt tcccagatat
      481 ggattggtca ccaatccctg aatcatgctg acgcgcgacc agtcgtaggc gcgttgcaat
      541 tcttcgtcgg gaagttcgac gctgactgtt ttggatagat agtcgcggta atagtcggtc
      601 gattggcgaa gcaattttac atattcagtg gtcaggtgtt gataggcggc ttccgcttgg
      661 gggcggcctt gcactgaagc ggccagcacg atcacttttg tgtctgagcc tttttccgta
      721 actcccagcc taaaggagtt gtcgctctcg ctggaatagt tggtttgata ttccctgcgg
      781 cgctcggcag cggtgggcga gccgaggaac gcggcgaatt ttttttgctc ttcgctgaag
      841 taaaacgcgt ttcgttctgg ctcccagttg acgtaggtgg cgcccaaggc ggcgggccac
      901 tccaattgaa aatcgcggcg aaatacggct tcgatttcca gcgggaattc tgtttcgact
      961 tcgaacgtta tgatcgcgcc gggctcatcg acgggaacaa aaaacgtttc tcgcacggaa
     1021 aaagtatcgc tggtgtaaac gatggtggtg gattccgggc ggacgattac ggtgcgtgcg
     1081 agagattccg cgggcagggc gcggtcttcg gtgtggaagc gtaaatgaaa atcgcgaaaa
     1141 atttttaggg ggtaaaccca ggcctccatg cgtccggact cgttgccaaa gattccggcg
     1201 cgtgttccga cggcggaaag aaattcccag gtacgtactg gccgggtcag ctccaaggag
     1261 ttggactgat cattatcctt acctttatcg gcggctgtgg caccggcagc gatgaagagg
     1321 aggcttgcca ggatgcgaaa taatttcata aaagcgcttg ccactatctc acaattgaat
     1381 tgacgtcgcg catggaaggg gtggcaatag caggcgcgtg caccggcggg cgcttgcaaa
     1441 ctgccatacg cctctctata aaggaaagcg gcagattcta gcacgagcca aagtcgggaa
     1501 ggtaccactc cttttgggtt tttcacgccg ctcttttctg aagtcgctga gccgatcggc
     1561 gacggtactg tcactagaaa acattttggg tttctcgcgt gctgcgactg tggaagcagt
     1621 tgtgcgcaaa atgccgcagg gcgctgccgc ggcagctgct tctattgcgg gaactccact
     1681 gggattgagt tttttgaatg ttgcaaagga agcggggctg aacgccaaga cgatttttgg
     1741 aggggagcat tcgaataaat atttgctgga aacgacaggg tgtggggtgg ccttttacga
     1801 ttacgacaac gatggatggc tggatatttt tttagtgaat ggctggcgat tggaaggatt
     1861 tcccgccgga caggaaccga cttctca
//
LOCUS       DATCBB010000369         3658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_110150, whole genome shotgun sequence.
ACCESSION   DATCBB010000369 DATCBB010000000
VERSION     DATCBB010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110150"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..662)
                     /locus_tag="VII37_04130"
     CDS             complement(174..662)
                     /locus_tag="VII37_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5068944.1"
                     /translation="MSCTRMETRLLGYIDGRLKEKEQREMETHLAACPTCMVRVNEFR
                     SVGSLLDELPQIEPSAAFDARVRARIAAEPVKQDWWSWLGAAPRVLVAASMLLLATVW
                     VGHRPAENANMASSGGSDETQIMQDQDLPVLENYDVLANFEPLTELPQPVSADDSAEQ
                     EM"
     gene            complement(686..1285)
                     /locus_tag="VII37_04135"
     CDS             complement(686..1285)
                     /locus_tag="VII37_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5068945.1"
                     /translation="MARTDVQLMLDVKAGDEQSFALLLHRYRTPLVNFLYRMVRNREQ
                     AEDLAQEVFIRVYRARADYVPSAKFTTWLFRIATNLALNSVRDTRHQRMEVSLDAPVT
                     VDSEEGDERPLDVAEKNPNIEEHLVQEAQRDMIRHAIDKLPEKQRAAVLLHKYQNLDY
                     GEISKILSCSESALKSLLFRAYETLRVELAPLVALRGKS"
     gene            complement(1366..2079)
                     /locus_tag="VII37_04140"
     CDS             complement(1366..2079)
                     /locus_tag="VII37_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY5068946.1"
                     /translation="MAAILGLVAAGGNLLGGYFVVYRHWQRRYLQYFVALGAGYMLAV
                     AFLDVIPESVRLAGDSAFLVILIGYFLIHLFEHTIAPHLHFGEETHHEVVSHQHVRAT
                     MLLGLAIHTFFDGVAIASGFLVSNWLGVVIFVAIFLHKLPEGFTVASVVLASGQSRRN
                     AILASGLLGAATLLGVLLTTLLESQLKYALPLSGGVTLYVAATDLLPEVNREPNWRVA
                     VLVFVGVASLLIMRRLFHV"
     gene            complement(2133..3203)
                     /gene="ychF"
                     /locus_tag="VII37_04145"
     CDS             complement(2133..3203)
                     /gene="ychF"
                     /locus_tag="VII37_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY5068947.1"
                     /translation="MQTGIIGLPQVGKTTLFRILTKASVEGKPGAQGTHVGVAKVPEP
                     RLVELAKLYNPKKITYATVQYVDVGGMQKDRNRDALAPLREVDTIAHVIRVFDDPSVP
                     HAAGSIDPLRDATNLELELILADHDQITRRLERLEKDLKKKKDPVLEAEKAVLDKCKA
                     HLESEKPLRELELTAEERKPIGGFLFLSQRPMLYVLNLGDDEAAELATAVERHKLSAL
                     QGRPNTAVVAICGRLEAELAQMEEKEAAELLESYGLKEPGLNRLIHATYDLMGLIPYF
                     TAGEPEVRAWTIRKGATAVKAAGEIHSDIEKGFIRAEVVRWNDLLAAGSIAAAREKAQ
                     VRLEGRDYIVQEGDVILFRHSG"
     gene            3432..>3658
                     /locus_tag="VII37_04150"
     CDS             3432..>3658
                     /locus_tag="VII37_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068948.1"
                     /translation="MTTTSPTLPFDLRIAKAALAAKDERLARLIEETVEFQIEMESMD
                     SPYEALMESIAYQSISGKAAATIFARIKALGT"
BASE COUNT          822 a         1185 c          884 g          767 t
ORIGIN      
        1 tgcgctcgcc tgatgcggcg tgggattagg agccgagtgc gtagcaggag tcggtgactg
       61 cggaggcctg tcgctgggat ccggacgctg cgcgagactc gaagccgcca agctcgcgcc
      121 gagacaaagc acgagcaggc aaccttcgaa ttttgtccgc agcgtcatcc gctttacatc
      181 tcctgttcag ctgaatcatc agccgaaacg ggttgcggca gttccgtcaa cggttcaaag
      241 ttagccagca catcgtaatt ttcaagcact ggcaaatcct gatcctgcat gatctgcgtt
      301 tcatcactgc caccagaact tgccatgttc gcgttttccg ccggacggtg gcccacccaa
      361 acagtggcca gcaacaacat ggaagccgcc accagcaccc ggggcgcggc gcccagccat
      421 gaccaccaat cttgcttgac cggttcagcg gcaatgcgcg cgcgcacgcg agcatcaaat
      481 gcggcagacg gctcaatctg cggcagttca tcaagcaagc tgccgacgga gcgaaattcg
      541 ttcacgcgca ccatgcacgt cgggcatgcc gcaagatgcg tctccatctc gcgctgttcc
      601 ttttctttca agcggccgtc gatatatccg agaagccgcg tttccattcg agtgcaactc
      661 ataactgcct tcccttccgt ggcccttaac ttttccctcg caacgccacg agcggggcta
      721 gttcaactcg caacgtttca tacgcccgaa aaagcagcga tttaagcgcg ctttccgagc
      781 acgacaaaat cttcgaaatc tcgccgtaat ccaaattctg atacttgtgc agcaacaccg
      841 cggcgcgctg cttttccgga agcttgtcta tcgcgtgccg gatcatgtcc cgctgcgcct
      901 cctgtaccag gtgctcctcg atgttggggt tcttctccgc cacatcgagc ggccgctcat
      961 cgccttcttc cgaatccact gtcaccggag cgtccagaga gacttccatg cgctgatgac
     1021 gcgtgtcccg gaccgaattc agcgccagat tcgtggcgat ccgaaacaac caggtggtga
     1081 acttcgcgct cggcacgtaa tccgcccgcg cccgatacac ccggatgaaa acctcttgtg
     1141 ccagatcctc tgcctgctcc cgattccgaa ccatgcggta gaggaaattg accagcggcg
     1201 tcctatagcg atgcaagaga agcgcgaacg actgttcgtc gcccgccttc acgtccagca
     1261 tcagttggac gtcggtcctg gccatagccg acacactgta tcaaacccgt gtattgtcag
     1321 aaagttgcaa atcgggaatg taaaactggc gggtcactag ctactttata catggaacaa
     1381 acgcctcatt atcagcaagc tggctacccc aacgaacacc agcaccgcca cccgccagtt
     1441 gggctcccgg ttcacctccg gcagcagatc cgtagccgca acgtacagcg ttacgccccc
     1501 cgacagcggc agtgcgtatt taagctggct ttccagaagc gtcgtgagca gcactcccaa
     1561 caacgtcgct gcccctagta accccgaagc caggatagcg ttgcgccggc tctgcccgct
     1621 ggccagcact accgaggcga cggtaaagcc ttcgggaagc ttatgcagaa aaatggccac
     1681 gaaaatcacc actcccaacc aattcgagac cagaaatccc gaagcgatcg ccactccatc
     1741 aaaaaaagta tgaatcgcca gccccaacaa catggtcgcg cgcacgtgct gatggctgac
     1801 tacttcgtga tgcgtctctt ccccaaaatg aaggtgtggc gcaatggtat gttcaaacag
     1861 atggatcagg aagtacccga tgagaatcac caggaacgcg ctgtcgcccg ccagtcgaac
     1921 cgactcagga atcacgtcca ggaacgccac cgccagcatg taacccgcgc ccaatgccac
     1981 aaaatattgc aggtaacgcc gctgccagtg gcgataaacg acgaagtagc cgcccagcaa
     2041 gttgccgccc gcagccacta gtccgagaat ggcggccaat gcgacgcgag ttgagtcagg
     2101 atgcggcatt tgctaagtca aagagagccg cgttaaccgc tgtgacggaa cagaattaca
     2161 tcgccttcct gcacgatgta gtcgcgccct tcgaggcgca cctgggcttt ttcgcgggca
     2221 gccgcaatgc tccccgcagc gagcaaatcg ttccaacgca ccacttccgc gcgaatgaag
     2281 cctttctcaa tgtcgctatg aatttctccc gcggccttca ccgccgtcgc cccttttcga
     2341 atcgtccacg cgcgtacttc cggctcgccg gcagtaaaat aaggaatcaa tcccatcaag
     2401 tcatacgtcg catgaatcaa gcgattcaag cctggctctt tcagcccgta cgattccagc
     2461 agttccgcgg cttctttttc ttccatttgc gcgagctccg cttccagccg cccgcaaatt
     2521 gccaccaccg cggtatttgg acgcccttgc agcgcgctca atttatgccg ctccaccgct
     2581 gtagcaagtt ccgcggcctc gtcatccccg agattcagca catacagcat gggccgctgc
     2641 gacaaaaaca aaaatccccc gataggcttg cgttcctccg cagtcagctc cagttcgcgc
     2701 agcggctttt cagattcaag atgcgctttg cacttatcca gcaccgcctt ctcagcctct
     2761 aaaaccggat ctttcttttt ctttaaatct ttctccagcc gctccagccg ccgcgtgatc
     2821 tgatcgtgat ccgccaaaat caattccaac tccaggttgg tagcatcacg caacggatca
     2881 atgctccccg ccgcgtgcgg caccgacgga tcgtcaaaca ctcgaatgac atgggcaata
     2941 gtgtccactt cacgcaacgg cgccagcgca tcccggttgc ggtctttctg catcccgccg
     3001 acgtccacat actgcaccgt ggcataggta atttttttag gattataaag tttcgccagc
     3061 tccacaagcc gcggctccgg aaccttggcg acgcccacat gcgttccttg cgcgccaggt
     3121 ttgccctcga cactagcttt agtaagaatt ctgaacagcg tggtttttcc gacctgcggc
     3181 agaccgataa ttcctgtttg cataaacgcg atatgttagc acagcgccgt cgccttcctt
     3241 atcgcgcgca aaatcggttt ttgggtgccc cacacgccgc aagttacgtg tggggctttt
     3301 gccaatgaac agggaaagtt cgggacgatt tcctcgaatc ccctctcaga gttttccaac
     3361 gttccgtttc tcgatactat gctcacacgc aacttagttt ttaggagcgc attcgctatg
     3421 cggattattc gatgaccacc acatccccga cacttccatt cgatttacgc atcgccaaag
     3481 ccgcactcgc cgccaaagac gagcgcctcg cccgtctcat cgaagaaacc gtcgaattcc
     3541 agatcgaaat ggaatccatg gacagccctt acgaagccct gatggaatcc atcgcctatc
     3601 aatccatctc cggcaaagcc gccgcaacaa ttttcgctcg catcaaagca ctgggcac
//
LOCUS       DATCBB010000370         1581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_272017, whole genome shotgun sequence.
ACCESSION   DATCBB010000370 DATCBB010000000
VERSION     DATCBB010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272017"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VII37_04155"
     CDS             <1..404
                     /locus_tag="VII37_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5068949.1"
                     /translation="IFFKIAGGIHPKFRTPGRALIFQGVLASLMALTGTFEELTNLFI
                     FAAWIFYGFAVVALFRMRRTEPDLPRPYRCWGYPWVPGLFVAGALALTIQIWIQRPGR
                     SSIGLLLILAGLPFYKMWARRERSSSGDVAA"
     gene            complement(350..1327)
                     /locus_tag="VII37_04160"
     CDS             complement(350..1327)
                     /locus_tag="VII37_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY5068950.1"
                     /translation="MRIVSLLPSATEILFALGLDAEVVGVSYDCDFPPQVSERRVVVD
                     SRIPPGLTSLEIDRRVREYVDRGESLYSVNADALRELAPDLVVTQDLCLVCAASPDDL
                     AGVLANFDKRPRVLSLNPRNLGDVWDDISRVARETSREHAAEKLLAEIQQRVQAVKHH
                     VEGISRRPRVAFLEWLEPFYVGGHWVPEMIALAGGEDVFGRVGAPSFRVELQDIVAAS
                     PDIIVVAPCGYDAKQARKEYCSMTFPSEWDAIPAVRDGRVYAFEANAYASRPGPRLVT
                     GVEALAKILHPEIVAGDEAVGAFELATPTRENYAATSPELDLSRRAHIL"
     gene            complement(1357..>1581)
                     /locus_tag="VII37_04165"
     CDS             complement(1357..>1581)
                     /locus_tag="VII37_04165"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068951.1"
                     /translation="KRAVRSMTMGMEIASILHQLYPDKFEVQKLLFLVGNDQTIRQLQ
                     EGLPAPEIVKAWETDLKTFEVMRRKYFLYK"
BASE COUNT          351 a          461 c          413 g          356 t
ORIGIN      
        1 gcattttttt taaaattgct ggcgggatcc acccaaaatt tcggaccccc ggccgcgcgt
       61 tgatttttca aggtgtgctt gcaagcttga tggcgctgac cggcacgttc gaggagctga
      121 ccaatctttt tatttttgcg gcatggattt tctacggctt cgcggtggtt gctctgtttc
      181 gtatgcggcg aaccgaaccg gatttgccta ggccgtatcg ttgttggggc tacccttggg
      241 ttccaggact tttcgtggcg ggcgcgctgg ccctgacgat acaaatttgg atccagcggc
      301 cgggtcgttc atcgattggg ctgcttttga tcctggccgg cctgcccttt tacaaaatgt
      361 gggcacggcg agagagatcg agctccggcg atgtcgctgc gtagttttcg cgcgtgggag
      421 tcgcgagctc aaacgcgccc acggcttcgt ctccagccac aatttctgga tgcaagattt
      481 ttgccagcgc ttcaacaccg gtaactaagc ggggcccggg ccgcgaagca taggcgttag
      541 cttcaaatgc ataaactcgg ccgtcccgta cggcgggaat cgcatcccac tcggaaggaa
      601 aagtcatgga gcaatattcc ttccgcgctt gcttggcgtc atagccacac ggggccacaa
      661 caataatgtc gggcgaagcg gcgacaatat cttggagctc cacgcgaaat gagggcgcac
      721 cgactcgtcc aaaaacatct tctccgccgg ccagtgcaat catctctgga acccagtgac
      781 cgccgacata gaacggttcc agccattcca gaaaagcgac acgcggccgc cgggaaattc
      841 cttcgacgtg gtgtttcacc gcttgcacac gctgctgaat ctcggctaac aatttctcgg
      901 cagcatgctc ccgcgaggtt tcgcgcgcca cccttgaaat gtcgtcccac acatcgccaa
      961 gatttcgcgg attcagcgag agcaccctgg ggcgtttatc aaaattcgcg agcacgccgg
     1021 ccaggtcatc aggagaagct gcgcaaacca aacacaaatc ctgggtgacc accaggtccg
     1081 gagccaattc cctgagcgca tcagcattga cgctgtagag gctttcaccg cggtctacat
     1141 actctcgaac ccggcggtct atctccagcg aagtcaggcc cggcggaata cgcgaatcga
     1201 ccaccacgcg ccgctcgctt acctgcggcg ggaaatcgca gtcgtaactt acgcccacaa
     1261 cttcggcatc aaggcccaac gcaaaaagaa tctcagtagc cgaaggcaat aacgaaacaa
     1321 tgcgcatctc aaaactttat cacttcgaag caggtttcac ttgtacaaga aatattttcg
     1381 ccgcatcacc tcgaaagtct tcaaatccgt ttcccaggcc ttaacaattt ccggagccgg
     1441 caggccttcc tgcagttgcc ggatcgtttg gtcgttgcca actagaaaca acaatttttg
     1501 tacttcgaat ttatctgggt aaagctggtg caatatgctc gcgatttcca tgcccatggt
     1561 catggaacgc acggcgcgct t
//
LOCUS       DATCBB010000371         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_206832, whole genome shotgun sequence.
ACCESSION   DATCBB010000371 DATCBB010000000
VERSION     DATCBB010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206832"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VII37_04170"
     CDS             <1..996
                     /locus_tag="VII37_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5068952.1"
                     /translation="SRESVLTLSTDLNKLADEIRLSAPNYFLNVPTLLERVRRGVLDS
                     ISKRAAPIRVLFKKAKESWERENGGTRLGPLDRMWLALGGRLIFAKVKERFGGNLRAL
                     ICGSAPLAVETQQFFMMLGIPVLQVYGLTETTAICTMDDPRAPVEPGYVGPAISGIEM
                     TLAENEEIVVRGPHIFPRYWNRPEETARVMKDGWFHTGDQGEKNKSGNWRIIGRIKNL
                     IILNSGHNVPPEPIEEKIAQRLARAQQVVVVGNGRGYLCALITGPVAGADVQKVINAI
                     NGDLPHYRQIRNFWLLPGVFTAEDGLLTANGKLRRDAIDRRFASEIATMYSGKSA"
     gene            993..1796
                     /locus_tag="VII37_04175"
     CDS             993..1796
                     /locus_tag="VII37_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5068953.1"
                     /translation="MKTFVGKTLRWNWNDGVVELTLDREPANEIGTAMLSDLEKFVAA
                     FDALAPETSVCIISSARKHGFSAGADLRELYEGAAQLDGAERLAGVRQFMERIHAVLN
                     AIDAAPFVTIAAVHGICFGGGLELALACDIIVADKMARFAFPELRLGLIPGFGGIPRL
                     KRDLGNGFVRDLLFTGRSVNATRAQAVGLVSQLVAEGEALNVARSNANQIKKFDPFTR
                     ASAKKFLKPIPHEELRREIDLFCELFTRPAVMAALKKFVESTDAMPYLP"
     gene            1834..2079
                     /locus_tag="VII37_04180"
     CDS             1834..2079
                     /locus_tag="VII37_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5068954.1"
                     /translation="MMRTVEQTTQEVLDLAAKHFQVAQGSLAADDDFFKKLGINSLQA
                     LELLSRLENHFGIELPDYEVQGVSDFRTLASRIQSRL"
     gene            2079..>2655
                     /locus_tag="VII37_04185"
     CDS             2079..>2655
                     /locus_tag="VII37_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY5068955.1"
                     /translation="MLDLSKYQSIGGALKDAVEVFANETCLIEADRERENHRLTYREF
                     RDRAMPVAKALQDAGFAASDRAAIIMTNQSKWLIGAYAIFYSGGMLVPLDYKLTPDEQ
                     WQLLKHSGARVLITEYPIWRQLGVAKGREAATELQLVIVTEAPIDTDLLGGVRWEIPS
                     THTGEPEFRPRMRNDAATIVYSSGTGGRPKGC"
BASE COUNT          592 a          637 c          849 g          577 t
ORIGIN      
        1 tcgcgggaaa gtgtgttgac gctttcgacg gatttgaaca agctggcgga cgagattcgg
       61 ctgagcgcgc caaattattt tttgaatgtg ccgacgctgc tggagcgagt gcggcgcggg
      121 gtgctggatt ccatttcgaa acgcgccgct cccattcgcg tgttgtttaa aaaagccaag
      181 gagagttggg agcgcgaaaa cggcgggact cgattgggcc cactggatag gatgtggctg
      241 gcgctaggcg ggcgtctgat ttttgcaaag gtgaaagagc gcttcggagg aaatttgcgc
      301 gcgctgattt gcggttcggc gccgctggcg gtggagacgc agcaattttt tatgatgctg
      361 ggcataccgg tgttgcaggt gtacggactg acggagacga ccgcgatctg taccatggat
      421 gatccgagag ctccggttga accgggctac gtggggccgg caataagcgg gatcgaaatg
      481 accttggctg agaacgaaga gatcgtagta cgcgggccac acatttttcc tagatattgg
      541 aaccggccgg aggaaacggc gcgagtgatg aaggacggat ggtttcatac cggagatcag
      601 ggggaaaaga acaagtccgg gaactggcga attatcggca gaataaaaaa tctgattatt
      661 ctgaattccg gacacaacgt gccgcccgag cctatcgaag aaaaaatcgc gcagcgactc
      721 gcgcgggccc agcaagtagt ggtagtgggt aacggccgtg ggtatttgtg cgcattgatc
      781 accgggccag ttgcaggagc ggatgtacaa aaagtgatca atgccatcaa tggtgattta
      841 cctcattacc ggcagattcg taatttttgg ctgctgccgg gcgtttttac ggcggaggat
      901 ggcctgctca ccgctaatgg gaaattgcgc cgtgacgcga ttgaccggcg cttcgcgtcc
      961 gaaattgcca ccatgtacag tgggaaatcg gcatgaaaac atttgtaggg aagacgctgc
     1021 gttggaattg gaatgatggc gtcgtggagt tgacgctcga tcgcgagccc gccaacgaaa
     1081 ttggaacggc tatgctcagc gatttggaga aattcgtcgc tgcgttcgat gcattagccc
     1141 ctgaaacttc cgtttgcatt atttccagcg cgcgaaagca tggattttcg gcgggtgccg
     1201 atctgcgcga gctttacgaa ggcgcggcgc agttggatgg cgcggaacgc ctcgcaggcg
     1261 tgcgccaatt tatggagcga attcacgcgg tattgaatgc tatcgacgcg gcaccgtttg
     1321 taaccattgc ggcggtgcat ggaatttgtt ttggcggcgg gctggaactc gcgctggcct
     1381 gcgacatcat agtggccgac aagatggcac gatttgcgtt tcctgagttg cgcctggggc
     1441 taataccggg attcggcggc ataccgcgat tgaagcggga cctgggtaac ggattcgtgc
     1501 gcgatttgct atttacgggg cgcagcgtaa atgctacgcg cgcgcaggcc gttggactcg
     1561 tatcgcaact cgtggcagaa ggcgaggctc tcaatgttgc ccgttcgaac gcgaaccaaa
     1621 tcaagaagtt tgatcccttc acgcgtgcgt cggcaaagaa attcctgaag cccattccgc
     1681 acgaggaatt acgccgtgaa atcgatttat tctgcgagct gtttacacgc cccgcggtga
     1741 tggccgcgct gaagaaattt gtcgaatcca cagacgcgat gccgtatctg ccgtaagact
     1801 gtggagtatg tttcagatag atataatgga gcgatgatgc ggacagtgga acaaacgact
     1861 caggaagtgc tggatctggc ggccaaacat tttcaggtgg cgcagggttc gctggcggcg
     1921 gacgacgatt tctttaaaaa gctggggatt aacagcctgc aagcgctcga attgctttcg
     1981 cggctggaga atcatttcgg cattgagttg cctgactacg aagtgcaggg cgtgagcgat
     2041 ttccgcacgc tggcgtcgcg catacaatcc cgcctgtaat gctggatctt tccaaatacc
     2101 aatctatcgg aggcgcgtta aaagacgccg tcgaggtgtt tgccaatgaa acctgcctca
     2161 ttgaagccga tcgcgagcgc gaaaatcacc ggctgacgta tcgcgaattt cgagaccgcg
     2221 ccatgccggt cgcgaaagcg ttgcaggacg cgggatttgc ggcgagcgat cgcgcggcga
     2281 ttatcatgac caatcagtcc aagtggttga ttggggcgta cgcgatattt tattccggcg
     2341 ggatgctggt gccgctcgat tacaaattga ccccggacga gcagtggcaa ttgctgaaac
     2401 actcgggcgc gcgcgtgctt atcacggaat atccgatctg gcggcaattg ggcgttgcga
     2461 agggccgcga ggcagccacg gaacttcagc tggtcatcgt tacggaagcc ccgattgaca
     2521 cggatttgct tggcggcgtg cgctgggaaa tacccagtac gcacactggt gagccggaat
     2581 ttcgaccgcg catgcgcaac gacgctgcga cgattgtgta ttcgtcggga accggcgggc
     2641 ggccaaaagg ctgcg
//
LOCUS       DATCBB010000372         1424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_336426, whole genome shotgun sequence.
ACCESSION   DATCBB010000372 DATCBB010000000
VERSION     DATCBB010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336426"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VII37_04190"
     CDS             complement(<1..272)
                     /locus_tag="VII37_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5068956.1"
                     /translation="MPAEKLFSPRQAAQVLGVSYPTLKTWIYKKKIRTIKTAGGHHRI
                     PESQLDKFLHQANERGDVAEKRTGFRHISGRNQLIGRVTDIKISGL"
     gene            413..931
                     /locus_tag="VII37_04195"
     CDS             413..931
                     /locus_tag="VII37_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5068957.1"
                     /translation="MTLGDKIRSLRSVEGSLRGLGRAMTQQELANAMKRETGRSLSQA
                     YLSQIESGSRPHLTHTTRELLARFFRIYPGFLVNDPEGYTPELQSEELRAIDAKIDSW
                     LHAGAEQFASDRELHGAFETIAKHEDSRQALLLLAEILRTPDLADRLTEVLRPNAKQG
                     SAHPPPGPTLPQ"
     gene            1210..>1424
                     /locus_tag="VII37_04200"
     CDS             1210..>1424
                     /locus_tag="VII37_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068958.1"
                     /translation="MSWFNASTAMAFLAWFGGIGYILSARSHLASMGRPDIARIRRDA
                     EVATAEAERDIAIKRAESLRASAVAKA"
BASE COUNT          273 a          430 c          400 g          321 t
ORIGIN      
        1 atcaagccgc tgattttgat gtccgtcacg cgaccgatca actgattgcg tccgctgata
       61 tgccggaagc cggtgcgctt ttcggcgacg tcgccgcgct cattggcttg gtgcaaaaac
      121 ttgtctagct gactttccgg aatgcggtgg tgcccgccgg cggttttgat ggtgcgaatt
      181 ttcttcttgt agatccacgt tttcagcgtc ggatagctga cgccgagaac ctgggcagct
      241 tgacgcgggg agaacagttt ttcagcaggc atgtgggctc cttgttcagg tccggttaat
      301 ccgccgatga gaataatgcc gtatgttcat tataataagc cagttatttt ttttgcttga
      361 caacatagca gattgcgatg tacatattgc acattgcaat tgtgaatttt ctatgactct
      421 cggcgacaaa attcgctccc tacgctccgt ggaaggctca ctgcgcggcc tagggcgtgc
      481 gatgacccaa caggaactgg ctaacgccat gaagcgcgag accggccgca gtttgagcca
      541 agcctacctc tcccagatcg agagcggctc gcggccgcac ctgacgcaca caaccagaga
      601 gctgctggca cgtttctttc gcatctaccc cggcttcctg gtgaacgacc cggaaggtta
      661 tacacccgaa ctgcaatcgg aagaactgcg ggccatcgac gccaagatcg attcctggct
      721 gcacgcggga gccgagcagt tcgcttcgga tcgcgaactg catggggcgt tcgaaaccat
      781 cgcgaaacac gaggattctc gacaggcatt gctgctgctc gcagaaattc tacggactcc
      841 ggatctggct gaccgtctaa ccgaagtgct gcgccccaat gccaaacaag gcagcgcgca
      901 ccctccaccc ggcccgactc ttccgcaata agccccgccg tgtgattccg cttcgagtcc
      961 cgcgtcgcgt gccgggtctc cagtaacaaa agtgcagttg cgggggtgtt ctatgtcctg
     1021 gtccgatttt tacctgatct gcttcttggt gggattctcg cttagcgtgc tttcgtttct
     1081 ggcgggcgca atccacatcc atcttcccgt ccgaatgcac atgccatttc atggcgcgta
     1141 tcacggcgca ggtgtgtggg ccctcactcc ggcgtggcca aggcaggttc gtcacactcc
     1201 agcgtacaca tgtcgtggtt caacgcctcc acggcaatgg cgttcctcgc ctggttcgga
     1261 ggaatcggct acatcctgtc ggcccgttct catctggcga gcatgggccg gccggacatc
     1321 gcacgcattc gccgcgatgc ggaagtggcc accgccgaag ccgagcggga catcgccatc
     1381 aagcgcgccg agtctctgcg cgcctccgcg gttgccaagg cgca
//
LOCUS       DATCBB010000373         5291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_110266, whole genome shotgun sequence.
ACCESSION   DATCBB010000373 DATCBB010000000
VERSION     DATCBB010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110266"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..2009
                     /locus_tag="VII37_04205"
     CDS             327..2009
                     /locus_tag="VII37_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY5068959.1"
                     /translation="MTWTQVYDPFGHWWLSTLVAAIPILVLLTLLAGFRVRAHLCAIA
                     GAAAAVIIAIVAFGMPARLAVISFLYGGAFGFLKIVWIVIAAVFLYDISVDTGQFEIM
                     KQSIAAITPDRRLQVLLVAFAFGAFLEGAAGFGAPVAIAGAFMIGLGFKPFQAAVLNL
                     IANTAPVAWGAIGTPVHVLAAVTALPESDLSAMVGRILPWTAVIVPFWLVRTFVSWSE
                     TFEVFPAILVVGISFAGVQYFWSNHVDSNLVDIAAGIASIVATLLFLRVWQPKRIWRF
                     EDERGEDAAKVKRGEVAVVHTPGQLAKAWMPFALLSLFVLLWGLPVIKTAMGKATTPA
                     FKTGGWAVPYLHQAVFRAQPVVMKPTAENAKFDLNWLTATGTGCFLAAILAGLLLGLK
                     PGRIFKIFGGTMYRLRFAIIAMACMLGLGYVTRYSGMDAVLGLAFTRTGWFFPFFGAY
                     IGWLGVALTGSDTSSNALFGGLQRITAQQLNLSPILMCATNSAGGVMGKMIDAQSICI
                     ATAATNQVGNEGSIFRFVFWHSVALAAIIGVIVMLYAYVFPQFIPHGLNFVH"
     gene            complement(2077..3255)
                     /locus_tag="VII37_04210"
     CDS             complement(2077..3255)
                     /locus_tag="VII37_04210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY5068960.1"
                     /translation="MPSVNSPHVVNIHDLRRVAQRRLPKVVFDYLDGGAEGEVTLREN
                     CRVFDDVTFRPRHAVAFANCDLRTTVLGHELSLPAMLAPVGYSRLMHPGGEVAAARAA
                     GKAGTGYILSTISGHKLENVKAGSTGPVFYQLYLMGGRPAAEASIDRARRAGFAALVI
                     TIDTPVSGIRERDPRNGMVELLNPGLFQKIPYAEQFLTHPGWLFSFLKDGGVPPLHNV
                     VLPGKGPMPLTDIGRALAASTVTWKDLGWIREHWKGPIVVKGVLTADDGRRAVDEGAA
                     AIVVSNHGGRQLDCVSATLRVLPEVVAAVNGRIEVLMDGGVRRGTDIVKALGLGARAV
                     LCGRAYAYGLAAAGETGVTRAIEILREDVKRTLVLLGCPNVAALDPSYVRIPKDWAAD
                     "
     gene            complement(3468..3896)
                     /locus_tag="VII37_04215"
     CDS             complement(3468..3896)
                     /locus_tag="VII37_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068961.1"
                     /translation="MNFQLGKVRHCAAVGVIVMSLGITVAPTSPAQEKRFPQTAGVDD
                     SKMGPYRALAQLAFAASQKGDNAMAAKLARILERDWDKAEDYGGESALSKTNHALFEE
                     IDKAMDHFIAPLVAHVSSAPDQAVVKAAYNTYLEKLKLAD"
     gene            4340..>5291
                     /locus_tag="VII37_04220"
     CDS             4340..>5291
                     /locus_tag="VII37_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068962.1"
                     /translation="MTPSKISCAWNHPEIAGNYRAAVSLHSHTNFSKESLHFIPAFAE
                     KWPVLRRALDKQCKKSCRPVDFSTAYWRPPVSAKVGFDLESKQIEHELDLMSLVSLTD
                     HDSMGAPTSLRQLSDGIQIPLAVEWSVPFEDAVFHLGIHNVPENQAPTIMEDFADYTR
                     KPCESRLAEILAALHEMPEILIVFNHPLWDQLGPGQLAFQLVLERFLQRHVEFLHAFE
                     LNAMRNWSENKRVVQLAERWQRPVVSGGDRHGCEPSGALNLTRASNFSTFVQEIREEQ
                     ASHVLFMPQYAEPRSLRFWQTVIDVLREYPEYPQGSRRWDE"
BASE COUNT         1114 a         1315 c         1570 g         1292 t
ORIGIN      
        1 ttccctgccc agctcgaggg ctttctcacc ggtgtcggcg atatccacag cgtaggaatt
       61 ttccctgagg gtgcgggcaa tgaagctggc aacttttcgt tcatcttcgg cgagtaggat
      121 acgcataagc ttgatgtgag cattatatcg cgatagctgg agttattgct tcagcggcta
      181 cgctggcgac aactgcgcgc caggattact tcgtgcttga caattcagcg gccaagcact
      241 atttcacaag cgcactactt ctaactggca gttcttccga aggctcgcaa ccctaaccgg
      301 tcgctattcc ttcggcggag gatcctgtga cctggacgca agtctatgac ccttttggtc
      361 attggtggtt atcgacgctc gtagccgcca taccaattct cgtgttgctg actcttttgg
      421 caggatttag agtacgtgcg catctttgcg ccatagctgg cgcggctgcg gccgttatca
      481 ttgcgatcgt ggcgttcggt atgccggcac ggttggcggt gatcagcttt ttgtatgggg
      541 gcgcgttcgg gtttttaaaa atcgtgtgga tcgtgattgc tgcggtgttt ctgtatgaca
      601 tttctgtgga taccggccaa tttgaaataa tgaagcaatc catagcggcc atcacacccg
      661 accggcggtt gcaggtgctg ctggtggcgt ttgcttttgg cgctttcctg gaaggagcag
      721 cggggttcgg ggcaccggtg gccatcgcgg gtgcgtttat gatcgggttg ggattcaagc
      781 catttcaggc tgccgttttg aacctaatag ccaataccgc gccggttgcg tggggggcga
      841 tcgggactcc ggtacatgtg ttggcggcgg tgaccgcgct tccagaatcg gatctaagcg
      901 cgatggtggg acggattctg ccgtggacgg cggtgatcgt accgttttgg ttggttcgga
      961 cttttgtgag ttggtcggag acattcgaag tatttccggc gattttggtg gtggggattt
     1021 ctttcgcggg agtgcagtat ttttggtcca atcatgtgga cagtaatttg gttgatatag
     1081 ccgcggggat tgcatccatc gtcgcgacac ttttattttt gcgggtgtgg cagccgaaac
     1141 gcatctggcg ttttgaggat gagcgtggag aagatgcggc caaggtaaaa cgcggagagg
     1201 tggcggtggt tcacacgccc ggccaacttg caaaagcttg gatgccattt gcgttgctgt
     1261 cgctatttgt tttgctttgg gggctgccgg tcatcaagac agcgatgggc aaagcgacga
     1321 ctccggcgtt taagactggc gggtgggcgg ttccatactt gcatcaagcc gtgtttcggg
     1381 ctcaaccagt ggtgatgaaa cctacggcgg aaaacgctaa gtttgatttg aattggctga
     1441 ctgcgactgg aacgggttgc ttcctggctg cgattcttgc tgggcttctg cttggtttaa
     1501 agccggggag aattttcaaa atatttggcg gcacaatgta ccgtttacgc ttcgcgatca
     1561 ttgcgatggc atgcatgctg gggcttgggt atgtcacaag gtattcgggc atggatgcgg
     1621 tattgggatt agcgtttacg cgaacgggct ggttctttcc gtttttcggc gcctatattg
     1681 gttggctggg agtggcgctg acgggaagcg acacttcatc gaatgctttg tttggcgggt
     1741 tgcaacgcat tacggcgcag caactcaacc tgagcccaat cttaatgtgc gccacaaaca
     1801 gcgcgggtgg cgtgatgggc aaaatgatcg atgcgcaatc aatctgcatt gccaccgcgg
     1861 caactaatca ggtcggcaat gaagggtcga tttttcgctt tgtgttttgg cactcggtag
     1921 cgctggcggc cattatcggc gtgatcgtga tgctgtacgc ttacgttttt ccgcaattta
     1981 taccgcacgg gttgaacttt gtgcactgaa cttcacgcac tgaaattagt acggtgctaa
     2041 ctttcgcaac aacgacctca gaaacgacgt accaattcaa tcagcggccc aatctttggg
     2101 gatgcgtacg taagaaggat ccagcgctgc gacgttggga cagcccaaca acaccagggt
     2161 gcgtttgacg tcttcacgca aaatctcgat ggcgcgggtt actcctgttt caccggcggc
     2221 ggctagtccg tacgcatagg cgcggccaca aagcacggct cgcgctccca ggcctagcgc
     2281 tttcacgata tcggtgccgc gccgcacgcc tccgtccatt aagacttcta ttcggccgtt
     2341 gaccgcggcc accacttcag gcaacacgcg cagcgttgcg gaaacacaat cgagttggcg
     2401 gccgccgtga ttcgaaacga cgatggcggc ggctccctca tcaacggcgc gcctgccgtc
     2461 atctgcggtg agtacacctt tgacgacgat tggacctttc cagtgttctc tgatccagcc
     2521 caaatccttc caagtcacgg tggaagcagc gagggcgcgg ccaatgtccg tgaggggcat
     2581 ggggcctttc ccgggcaaaa ccacgttatg gaggggaggg actccaccat cctttaagaa
     2641 cgaaaatagc cagcccggat gggttaagaa ttgttcggcg taaggaattt tctggaacaa
     2701 gcccggattc agcagctcaa ccatgccgtt acggggatcg cgctcgcgaa ttccactgac
     2761 aggagtgtcg atggtaatca ccagcgccgc aaatcctgcg cggcgcgcgc gatcgattga
     2821 ggcttccgca gcggggcgtc cgcccatgag ataaagctgg tagaagaccg gtccggtcga
     2881 gcctgctttt acattttcga gtttgtgtcc tgaaatagtg gaaaggatgt aaccggtgcc
     2941 ggctttccct gccgcgcgcg cggcggcaac ttctccgcca ggatgcatga gccggctata
     3001 gccgacggga gccagcattg ccggaagtga aagttcgtgg ccaagcacgg tggtgcgcaa
     3061 atcgcaattc gcgaaggcta ccgcgtggcg gggacgaaag gttacatcat caaaaacacg
     3121 gcaattctca cgtagagtga cttcgccttc tgcgccgcca tccagataat cgaaaaccac
     3181 tttggggagc cgccgctgcg ctacgcgcct caaatcatgg atattcacga catgcggcga
     3241 gtttacggaa ggcattcgca ttccctccac gggaatcgga gaatcgcgca aacaattaag
     3301 ccacaattcg gcttttgacg cgatacggga atgaccctga gtatttacga gagtgccttt
     3361 cgtgcagggg tgccacgctg aggggagcgg acctaactaa gaacgtaggc gagaatgaag
     3421 ctaccgccct aaattatctt gataagttga tgtgcgtcgg attgttatca gtccgctaat
     3481 tttaattttt ccaagtaggt gttatatgcg gcctttacca cggcctgatc cggtgctgag
     3541 gaaacatgcg caactagcgg tgcgataaag tggtccatgg ccttatcgat ttcctcgaac
     3601 agggcgtggt tggtcttgga gagtgcggac tcaccgccat aatcttctgc tttgtcccag
     3661 tcgcgctcga ggatgcgcgc gagttttgcg gccattgcgt tgtcaccttt ttgggacgca
     3721 gcaaacgcga gttgagccag ggcccggtat ggacccatct tgctatcgtc tactccggcg
     3781 gtttgtggaa agcgcttttc ctgggccggg gatgtcggag ccacggtaat gccgagcgac
     3841 atgacgatta cgcccaccgc ggcgcagtga cggactttgc ctaattggaa attcacctgt
     3901 gatcctcctt cgctctaaga gggtagttgc gttacaggga tagtacattt ctaagcattc
     3961 agcctgcgaa gtatttgctg gaaaaaaccg taaagagtaa ccatcgcagc aacggctatc
     4021 ggtcttcggt gctgcgcatg gcggtgtcaa ccaaaggtag atctgcggcc atgtgttcac
     4081 aaatgcggct tgcattgccg gactgagaga tagatggctg ccagtgtttt catttcgtga
     4141 ccacgattct gattggtccg tatgtgtgca attgtggata ttttcgtatt ggcaactgct
     4201 gtataccaaa tgcttgagga aaagactgga ataacggtta gaataactac agtgacggtc
     4261 tgtaggaagc gtcggcctcc aggtagttag acccagatcg ggtatgtgcc gcagccgcgg
     4321 ctaaaattgg aggcagcgat tgacgccctc gaagatttcg tgcgcttgga atcacccaga
     4381 aatcgcggga aattaccgcg cagcggtttc tctccacagt cacactaatt tctccaaaga
     4441 aagcctgcac tttatcccgg cattcgctga gaagtggccg gtgttgcgtc gtgctctaga
     4501 caaacagtgc aagaagtcgt gcagaccggt ggatttttct acggcgtact ggcgccctcc
     4561 agtgtcggcc aaagtgggat tcgatttgga gagtaagcag attgaacacg agctggattt
     4621 aatgagtctc gtatcgctta ctgaccatga cagcatggga gcgccgacgt ctttgcggca
     4681 actgtccgac ggtatacaga tcccactggc ggtggagtgg agtgttcctt ttgaagacgc
     4741 agtctttcat ctagggatcc acaacgtgcc ggagaaccag gccccgacga tcatggaaga
     4801 ctttgcggac tatacgcgca agccttgcga atcccgtctg gcggaaatac tggcggcgct
     4861 ccatgaaatg ccggaaattc tgatcgtgtt caatcaccct ctttgggacc agttggggcc
     4921 tggacaactt gcattccagc ttgttctgga acgatttttg cagcggcacg ttgaatttct
     4981 ccacgctttc gaactcaacg ccatgcgcaa ctggagcgaa aacaaaagag tcgtgcagtt
     5041 ggcggagcgt tggcaacggc cggtcgtttc aggaggagac cggcatgggt gcgaaccgag
     5101 tggagcgctg aacctgacac gggcgagcaa tttctctact ttcgtacagg aaatcagaga
     5161 agagcaagcg agtcatgttc tgtttatgcc gcaatatgcg gagccgcgca gcttgcgctt
     5221 ttggcaaacg gtgattgatg tgcttcgcga gtatccggaa tatccgcagg gttcgcgtcg
     5281 ctgggatgag c
//
LOCUS       DATCBB010000374         1931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_110273, whole genome shotgun sequence.
ACCESSION   DATCBB010000374 DATCBB010000000
VERSION     DATCBB010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110273"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VII37_04225"
     CDS             <1..419
                     /locus_tag="VII37_04225"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEY5068963.1"
                     /translation="RFLAFFLWRASTKNVQSGAGSQDAVGPDERAYTSNVGVDHVELS
                     RAENFLHQEVTTISGQISNGGNRPLASVELTIEFYDDLNQIAQRETRSLIGTPGPPIP
                     PGDHREFEVSFEHISSSWNMRQPAIKVTKVQLFNQK"
     gene            435..1094
                     /locus_tag="VII37_04230"
     CDS             435..1094
                     /locus_tag="VII37_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068964.1"
                     /translation="MLPLYSALVLAVERFGRSRPDRLEFVRTDFERSARELSREEFCE
                     GLKELLASQFPDETVESATISPDLEHSLSGNYVRGLSKRGSRYLAFLAVPDDESQDTV
                     NNSLTFALLWLDRLRNGNHRGTVEGVRLIVPKSSCSVVAHRFAALGPGLAIELYERDA
                     ARESLERMDPRGAGNIHTWLVPRRESEALFAQARSDIEPIVASDPQAITIHPSTTARE
                     R"
     gene            1097..1723
                     /locus_tag="VII37_04235"
     CDS             1097..1723
                     /locus_tag="VII37_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068965.1"
                     /translation="MRYRGLAFARWDDGRIFFGCNDMRKELTPASRVAFERMMEDLGI
                     YRHPLASDTRHPLYRAQAERWLESLVRNDVTRVDSILDSRFVYAQVFAGSGAEHGIID
                     VLTITRAGRLAILELKASEHIHLPLHSADYWLRVRRHLELGEFRSYGYFPGVEIQQAP
                     PLVYLVAPALRFHPVTGNLLDALSPQIEVVRVGLTESWRRGIRVVMRE"
BASE COUNT          392 a          522 c          549 g          468 t
ORIGIN      
        1 tccgatttct ggcatttttt ctgtggcgtg catcaacgaa aaatgttcag tctggggcgg
       61 gatcgcaaga cgcggtgggg ccggatgagc gcgcctatac gtccaatgtg ggggtggatc
      121 acgttgagct tagccgcgcg gaaaatttcc tgcaccagga agtcaccaca atttccggac
      181 aaatcagtaa cggtggcaat cgtccgctgg ccagcgtgga gttgaccatc gaattttatg
      241 acgatctcaa tcagatcgcg cagcgcgaaa cgcggagttt aattgggact ccgggcccgc
      301 caattccacc cggagatcat cgcgagtttg aagtttcgtt cgagcacatt tcctcgtcct
      361 ggaacatgcg gcagccggca atcaaggtaa ccaaagtgca attatttaac caaaaataat
      421 atctattccc ttacgtgctt cctttatatt cggcgctggt tctggccgta gagcgctttg
      481 ggcgttctcg cccggatcgt ctcgagtttg tgcgtaccga tttcgagcgt tccgcacgtg
      541 agttgtcgcg tgaagagttt tgcgaaggcc tcaaggaact gctggccagt caatttcccg
      601 atgaaaccgt cgagtccgca acgatttccc cggacctgga gcattctttg tccggcaatt
      661 atgtccgcgg attgtccaaa cgcggttccc gctacctggc ttttctggcc gtgccagatg
      721 acgaatctca agacaccgta aataacagcc tcacgtttgc gttgctatgg ctggatcgtc
      781 tgcgcaacgg aaaccaccgt ggaacggtag agggcgttcg attgatcgtt ccaaagagtt
      841 cctgtagcgt ggtggcgcat cgctttgctg ctctcgggcc cggcctggca attgagcttt
      901 acgagcggga tgctgcgcgc gaatcgctgg agagaatgga tccgcgtggc gctgggaata
      961 ttcatacctg gttagtgccg cgccgagaat cggaagcgct tttcgctcag gctcgttcgg
     1021 acatcgagcc cattgtggca tccgacccgc aggccattac catccatccc agcactaccg
     1081 cgcgcgagag gtagttttgc gttatcgcgg cctcgcgttt gcgcgctggg atgacggaag
     1141 aatctttttt ggctgtaacg atatgcgcaa agaactgact ccggcttccc gggtggcgtt
     1201 cgaacgcatg atggaggatc tgggaattta ccggcatccg ctcgcctccg acacgcgtca
     1261 tcctttatat cgcgcgcagg cggaacgttg gcttgagtcg ctggtgcgca acgacgtaac
     1321 tcgcgtggac agcattctcg attctcgctt tgtttatgct caagtctttg cgggcagcgg
     1381 cgcggagcat ggcattattg atgtgctgac gattacacgg gcgggccgtc ttgccattct
     1441 ggaattaaaa gccagcgagc acattcatct cccgttacac tcagcggatt actggctgcg
     1501 cgtgcgccgc catttggagc tcggcgagtt tcgcagctat ggctattttc ctggcgttga
     1561 aattcaacaa gcgccgccac tggtttattt ggttgctccc gcgctgcgct tccacccggt
     1621 gaccggcaat ttgctggatg cgctgtcgcc acaaatcgaa gtggttcgcg tgggcttgac
     1681 ggaaagttgg cggcgtggca tccgcgtagt catgcgcgaa tagccaaccc acaagtcgca
     1741 attgccatct attcggtgtt agccagatca tttagatgag gtgcgacttg aaaacatatc
     1801 cgctagcaat cgccatctgg gccctatgct tcgtgattgc cgccgcgtca gcccaaccct
     1861 cgccttccag ggccaaatta actgagaatc cggtgtacaa ggagaagtgc gcgaagtgtc
     1921 acggaaagac t
//
LOCUS       DATCBB010000375         1651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_238631, whole genome shotgun sequence.
ACCESSION   DATCBB010000375 DATCBB010000000
VERSION     DATCBB010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238631"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VII37_04240"
     CDS             complement(<1..240)
                     /locus_tag="VII37_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5068966.1"
                     /translation="MTDSSSLIGQTISHYRILEKLGGGGMGVVYKAQDTRLDRFVALK
                     FLPDNVARDPQTLSRFRREAKAASALNHPNICTIYE"
     gene            402..>1651
                     /locus_tag="VII37_04245"
     CDS             402..>1651
                     /locus_tag="VII37_04245"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5068967.1"
                     /translation="MKLFVLKLGVLLLLAAAFTSGLIPAQEKAEQQRAPEQQPAAAAI
                     VIVNAQVSDGSDAPLKKVSVRIAGGRITKIGVFKPAKGEQIIDAQGLVLAPGFIDIHN
                     HSAEGLDKDPLAETQISQGITTVILGPDGFSPWPIGAWVESRRKAPAALNVATLFGHA
                     TLRSQVMGKDFRREATPEEIQKMVALSEDAMNEGALGVSTGLEYDVASYSSTAEVVAL
                     AEVAAKHAGFYETHVRDEGNKSFSALEEEIAIGQRAHLPIEHSHIKVSTVAVWGKASE
                     YISVIEAARKRGLDFLADCYPYDAWHSNIKVLMPDKQYTNPQSVEKALTDTGGPDTVT
                     ITEFAPHPEYVGRSIAELAAARKISPTEMYIQIIREGDAANTEASIIGKSMIERDIQA
                     FYQQPWVMVASDGGIGAQHPRGAGT"
BASE COUNT          374 a          387 c          531 g          359 t
ORIGIN      
        1 ttcgtagatg gtgcaaatat tgggatgatt cagggcagag gcggccttgg cttcgcggcg
       61 gaatcggctt agcgtttgcg ggtcgcgcgc gacattgtca ggcagaaatt tcaaggcgac
      121 aaagcggtcc aggcgggtgt cctgggcctt gtaaaccacg cccatgcctc cgcccccgag
      181 tttttcgagg atgcggtaat gggaaatggt ctgaccgatc agggaggagg aatctgtcat
      241 tcgtcgctca tcttaggttg tggcgggaaa cgagtcaacg aacgtcagta ttcatggctt
      301 gttggtttta cgggtctgct aggtcgcatc gttacctttc caaaaaccaa tgcatccatg
      361 aggcatgatt tttgtcatac tgctgcgatc tgagggtgag tatgaaattg tttgttttaa
      421 aacttggggt gctgttgctc cttgctgctg cgtttacatc ggggcttatt ccggcacagg
      481 aaaaagcaga gcagcagcga gcgccggagc agcagccggc ggctgcagcg atcgtgatcg
      541 tcaatgcaca agtgtctgat ggcagtgacg cgccgctgaa aaaagtcagc gtgcgcattg
      601 cgggtggtcg catcacgaag attggggtgt tcaagcctgc taagggtgaa cagattattg
      661 atgcgcaagg gctggtgctt gcgcctggat tcatcgacat tcataaccat tcggcggaag
      721 ggctggacaa ggatccgctg gcggagacgc aaatttcgca gggcatcacc acggtgattc
      781 ttggtcccga tggtttctcg ccgtggccca ttggcgcatg ggtggagagt cggcgcaaag
      841 cgcctgctgc gctgaatgtc gcaacacttt tcgggcatgc cacgctgcga tcccaagtga
      901 tggggaagga ttttcggcgc gaagccacac cggaggaaat tcaaaaaatg gtggcattgt
      961 ccgaagatgc catgaatgaa ggggcgctgg gcgtgtccac cggtcttgaa tacgatgtgg
     1021 ccagctacag ctccaccgcg gaagttgtgg cgctggcgga agtggcggcg aaacatgcgg
     1081 gcttttatga gacgcacgtg cgtgatgagg ggaataaaag tttttcggcg ctggaagaag
     1141 aaattgctat cggacagcgc gcgcatctgc ccatcgagca ttcgcatatc aaagtgagta
     1201 ccgtggcggt gtgggggaaa gcgtccgagt acattagcgt gatcgaagcg gcgcgcaaaa
     1261 ggggcttgga ttttctggcc gattgttatc cgtatgacgc gtggcattcc aatatcaaag
     1321 tgctcatgcc ggacaaacaa tatacaaacc cgcaaagcgt tgaaaaagcg ctgacggata
     1381 ctggcggacc ggacaccgtg acgattacgg aattcgctcc acatcctgaa tacgtggggc
     1441 gcagcatcgc ggagctggcg gcggcgcgga aaatttcgcc tacggaaatg tacattcaga
     1501 ttattcgtga aggcgatgcc gccaacacgg aagcgagcat catcggcaaa tccatgatcg
     1561 agcgggacat tcaggctttt taccagcagc cgtgggtaat ggtggcgagc gatggcggaa
     1621 ttggtgcgca gcacccgcgc ggtgcgggga c
//
LOCUS       DATCBB010000376         1353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_402089, whole genome shotgun sequence.
ACCESSION   DATCBB010000376 DATCBB010000000
VERSION     DATCBB010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402089"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VII37_04250"
     CDS             complement(<1..814)
                     /locus_tag="VII37_04250"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY5068968.1"
                     /translation="MKALLLLAVVLTGGLPFLATNAQSPPDSGAPDSGAHGSGKSASG
                     KSEKVGYVGFDRNQYPGDANLKILRQSFSYSGYWLNNPPGAKANSWKGKRQALQDAGF
                     GFLVLFNGRTYAEIKRAGNATKLGTSDAAAAIAAASAEGFPLHTVIFLDQEQGGRLLP
                     EQRAYLHAWVDAVSSASFVAGIYCSGIAAKEGSGESVITAQDIRNNAGNRKIKFWVVN
                     DSCPPSPGCTVSVSKTDLMPELSGTSFSDVWQFAQSPKRRDFAKGCPANYNKD"
     gene            complement(845..>1353)
                     /locus_tag="VII37_04255"
     CDS             complement(845..>1353)
                     /locus_tag="VII37_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5068969.1"
                     /translation="RDLRLEDVDLRKILESVSVLAAPDAARHGVQLVQQLPPEDDALM
                     VRVDPDLMKQAILNLVLNGVQSMTSGGTLTLRARRDDEMILAEVMDQGCGIPPDAQEK
                     IFELYFTTKGEEGGSGIGLAQTYQIMQWHYGSIDFDTVVGAGTTFRMRLPATLARPDR
                     RADEEITA"
BASE COUNT          294 a          407 c          361 g          291 t
ORIGIN      
        1 catccttgtt gtaattcgcc ggacagccct ttgcgaagtc ccggcgtttt ggcgattgag
       61 cgaattgcca cacatccgaa aacgaagtgc cgctcaattc cggcatcaaa tcagtcttcg
      121 aaacagaaac agtgcatccg ggcgacggcg gacaggaatc gttgaccacc caaaatttaa
      181 tctttcggtt acccgcgtta ttgcggatgt cttgcgccgt aatgacggac tcgccggaac
      241 cttctttagc agcgatgcct gagcaataaa tgcctgcgac aaaactcgcc gaactgacgg
      301 catcgaccca ggcatgaaga taggctcgct gctccggaag cagacggccc ccttgttcct
      361 gatcgagaaa gatcacggtg tgaaggggaa atccctcagc gctagctgct gcaatcgccg
      421 cggcagcgtc cgacgttcct agcttcgtcg cattccccgc acgtttaatt tccgcgtaag
      481 tgcgcccgtt gaagagaacg aggaacccga agcccgcatc ctgcagagct tgccgctttc
      541 ctttccagga attcgccttc gctccgggtg gattattcag ccaatagccg gagtaagaaa
      601 aactctggcg aagaatcttc agattcgcgt cgcccggata ctggttgcga tcgaatccta
      661 cgtatccaac tttttcagat ttaccggaag cagatttacc ggaaccatgt gcgccggaat
      721 cgggtgcgcc ggaatccggc ggtgattgag cattggttgc taagaatgga aggccgccgg
      781 tgaggactac ggcaagcagc aacaaagctt tcatttcggc tggctcctta taccccgatg
      841 cggctcaggc ggtgatttct tcatctgcgc ggcgatcagg tcgcgccagc gttgccggca
      901 ggcgcatgcg gaatgtcgtt cctgctccca ccacagtatc gaagtcgatt gagccataat
      961 gccactgcat aatctggtag gtttgcgcga gcccaatgcc gctcccgcct tcttcgccct
     1021 tggtggtgaa atagagttcg aagattttct cctgcgcatc gggcggaatg ccgcagccct
     1081 ggtccatcac ttcagcaaga atcatttcgt catcgcgccg cgcccgcagc gtgagcgtgc
     1141 ccccgctcgt catggattga acgccgttca gcaccagatt caaaatcgcc tgcttcatca
     1201 aatcgggatc gacccgcacc atcagggcgt cgtcttccgg cggcaactgc tgcactaact
     1261 gcacgccatg acgggcggca tcgggcgcgg ccagcaccga cacactctcc aaaatcttac
     1321 gcaggtccac atcttccagg cgaaggtcgc gcg
//
LOCUS       DATCBB010000377         1147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_272243, whole genome shotgun sequence.
ACCESSION   DATCBB010000377 DATCBB010000000
VERSION     DATCBB010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272243"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VII37_04260"
     CDS             <1..559
                     /locus_tag="VII37_04260"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY5068970.1"
                     /translation="TYALFTIPGEDEWTIIINKGAEQWGAFQYDEKADVARFKATPTT
                     LGKSIDTFTIEFNQIRDESAYLNLVWDKTVVPIKMEIELTGKLVPQIEAVMASDAKPK
                     PYYQAALFYYNHGLDLQKADKWVDAAIAEREAHYIVYLKAEILAKLGDKEGAIAAAKR
                     SSELAVKAKDTGYVKLNDDLISSLK"
     gene            631..>1147
                     /locus_tag="VII37_04265"
     CDS             631..>1147
                     /locus_tag="VII37_04265"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5068971.1"
                     /translation="MAASIFTPVGFAAGPQIGPSLAILHDLQSFRQMGSVLYIAAHPD
                     DENTELIAYLARGRDYRTAYLSLTRGDGGQNVLGPDFGDKLGMARTQELLAARRIDGG
                     RQFFSRAVDFGFSKDYRETLNIWDKPEVLSDIVRVIREFRPDVIITRFSPIPGGTHGH
                     HTASTVLALEAF"
BASE COUNT          258 a          293 c          319 g          277 t
ORIGIN      
        1 cacttacgcg ctcttcacga ttcccggcga agatgaatgg acgatcatca tcaacaaggg
       61 cgcggaacaa tggggcgctt ttcagtatga cgaaaaagcc gacgtcgcgc ggttcaaagc
      121 cacgccgact acgttaggaa aatcaattga taccttcacc attgagttca accaaattcg
      181 cgacgagtcg gcttatttga atttggtctg ggacaagacg gttgttccca taaaaatgga
      241 aattgaactg acgggcaaac tcgttccgca gattgaggcg gtgatggctt ccgatgcgaa
      301 accgaaacct tattaccagg cggccctgtt ttattataat cacgggctgg atttacagaa
      361 ggccgacaaa tgggtggatg ccgccatcgc cgagcgcgag gcgcattaca tcgtttatct
      421 aaaggcagaa attctggcca aattgggtga taaggaaggt gccattgctg cagccaaacg
      481 ctcgagcgag ctggccgtca aagccaaaga cactggctat gtgaagctga atgacgactt
      541 gatctccagc ttgaaatgat gactgtcatt tccgcttcgg tgaagcattt gtgcaaattt
      601 cggcaggcat tttggtgtgg cctggtttgc atggcggctt caatttttac gccagtgggt
      661 ttcgctgccg gcccgcaaat tggcccgtcg ttggccattc ttcatgacct gcaaagtttc
      721 cgccagatgg gcagcgtgct ttatatcgcg gcgcatcctg atgacgagaa tacggagctg
      781 atcgcctatc tcgcgcgcgg gcgcgattat cgcacggcct acctttcgct cacccgcggc
      841 gatggcggtc aaaatgtcct cgggccggat ttcggtgata aattggggat ggcgcgcacg
      901 caagaattgc tcgcggcccg gcggattgat ggcggccggc aatttttttc gcgcgccgtt
      961 gacttcggct tttccaagga ttatcgcgag acgctcaaca tttgggacaa accggaagtg
     1021 ttgtcggata tcgtgcgcgt cattcgtgaa tttcgtcctg acgtgatcat cacccgcttt
     1081 tctccgattc cgggaggcac gcatggccat cacaccgcgt caaccgtatt ggcgcttgaa
     1141 gcgttca
//
LOCUS       DATCBB010000378         1305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_77055, whole genome shotgun sequence.
ACCESSION   DATCBB010000378 DATCBB010000000
VERSION     DATCBB010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77055"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>1305
                     /locus_tag="VII37_04270"
     CDS             21..>1305
                     /locus_tag="VII37_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068972.1"
                     /translation="MRRDRKTGDEDEIISQLESAFRPTLSPDGKQMVYVTRYETETGL
                     RLRNLETGEDHWVKYPVTRDDQEARFTRDVFPGLAFLPGGKEIVYNQDGKIRRLDLAS
                     GSEKIIPFTAQVSQDIGPKLDFPQRVEQGPVKVRLIMDPVESPDSKRLAFSAMTHLYT
                     MDLPHGQPQLLTSGNGHEFQPAWSPDGKFIAYVTWSSEGGQLWKVPSGGGTPVQLSKS
                     LAVYSNPAWSPDGSKIVALRGNAYDRENSEFDGGQTGNADLVWIPSDGGEANLILPAR
                     GAGGPHFTHEKDRVYVYTPQGLVSLRYDGTDRRTHLQVKGQGLYFFEEPIPADDVQPS
                     PDGQWVLAHVMNQLYLIAMPVVGGEAPTVNVSTAAVPAKRLTDIGADYFAWADDGKTI
                     TWAVGSSLFREPLSAISFEPPKDEKKDEDKKDGDKK"
BASE COUNT          288 a          264 c          474 g          279 t
ORIGIN      
        1 agctgccact gtggaagatc gtgcgacggg accggaagac cggtgatgaa gatgaaatta
       61 tttcgcaact ggaaagcgcg tttcgtccga cattgtcgcc ggatggaaag cagatggtgt
      121 atgtgacgcg ttacgaaacg gaaacggggt tgcggctgcg caatctggaa acgggggaag
      181 accactgggt gaaatatccg gtgacgcgcg acgatcagga ggcgcgattt acgcgtgacg
      241 tgtttccggg gttggcgttt ttaccgggag gaaaagaaat tgtttacaac caggatggga
      301 agattcggcg actggatctg gcgagcggga gcgagaaaat aattccgttc acggcgcagg
      361 tttcgcagga cattgggccg aagctggatt ttccgcagcg ggtggaacaa gggccggtga
      421 aggtgcgctt gatcatggat ccggtggagt cgccggacag caagcggctg gcgttttctg
      481 cgatgacgca tttgtacacc atggatttgc cgcacgggca gccgcaactt ttgaccagcg
      541 gaaatgggca tgagtttcaa ccggcgtggt cgccggacgg aaaatttatt gcgtatgtga
      601 cgtggtcgag tgaaggcggg caattgtgga aggtgccgtc tggtggtggc acgccggtgc
      661 aattgtcgaa atcgctggcg gtgtacagca atccggcgtg gtcgccggat ggatcgaaaa
      721 ttgtagcgct gcgcgggaat gcgtatgacc gcgagaatag cgagtttgat ggggggcaaa
      781 cgggcaatgc ggatctggtg tggattccct cggacggcgg ggaagctaat ttaattttgc
      841 cggcgcgagg agcgggaggg ccgcatttta cgcacgagaa ggatcgggtt tatgtgtaca
      901 cgccgcaggg gttggtttcg ttgcgctatg acgggacgga ccggcggacg cacctgcagg
      961 tgaagggaca gggactttat ttttttgaag agccgattcc ggctgatgat gtgcagccta
     1021 gtcccgatgg gcaatgggtt ttggcgcacg tgatgaatca gctttatttg atcgccatgc
     1081 cggtggtggg aggcgaggcg cctacggtga acgtgagtac ggcggcggtg ccggcgaagc
     1141 gcttgacgga tattggggcg gattattttg cttgggcgga tgacgggaag acgattacgt
     1201 gggcggtagg gtcgagtttg tttcgcgagc ctttgagcgc gatttctttc gagccgccta
     1261 aagatgaaaa gaaggacgaa gataaaaaag atggcgacaa gaaag
//
LOCUS       DATCBB010000379         1215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_272366, whole genome shotgun sequence.
ACCESSION   DATCBB010000379 DATCBB010000000
VERSION     DATCBB010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272366"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VII37_04275"
     CDS             complement(<1..827)
                     /locus_tag="VII37_04275"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5068973.1"
                     /translation="MQREITALWGEKKVVLRQTPRAVTPFGGLSVFVAFLGKIGYQQQ
                     VSEHLPVHLKSPNAIDPGETFTAFLISVVAGARRFAHTAMLRTDKALHALLGIQRFPT
                     DDTVRNLFKRFRQGLVVQFYEPLWSWQLARVPKRAGGYSLDLDSTVFERYGEQEGAKR
                     GYNPRKHGRASHHPLLAVLGEAQFILHGWLRSGNTRSDSGVVEFLKEAWAKLESRKWI
                     RVVRADSGFFAQELLQYLEDLELHYIVVARLTKWLKREAARVQTWRALDETYAVGEFR
                     "
     gene            913..1023
                     /locus_tag="VII37_04280"
                     /pseudo
     CDS             913..1023
                     /locus_tag="VII37_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1020..1154)
                     /locus_tag="VII37_04285"
     CDS             complement(1020..1154)
                     /locus_tag="VII37_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068974.1"
                     /translation="MCSLKKATMMLLVVLTMTAGLWAQHPGHGGMGGMFGNMPSMPSA
                     "
BASE COUNT          261 a          424 c          281 g          249 t
ORIGIN      
        1 cggaactctc ccaccgcgta ggtctcgtcc agcgcccgcc acgtttgcac ccgcgccgcc
       61 tcccgcttca gccacttcgt caggcgcgcc accacgatgt agtgcagctc caaatcctcc
      121 aggtattgca atagttcttg cgcgaagaac ccggagtccg cacggaccac ccgaatccat
      181 ttccggcttt ccagtttcgc ccacgcttcc ttcagaaact ccaccacccc gctgtcggag
      241 cgcgtgttcc cgctacgcag ccagccgtgc agaataaact gggcttctcc caacaccgcc
      301 agcagcggat ggtgtgaagc ccgcccgtgc ttgcgcgggt tgtaccctcg cttcgctccc
      361 tcctgttccc cgtagcgctc gaacaccgtc gagtccaggt ccagactgta accccccgca
      421 cgtttcggca cccgcgccaa ttgccacgac cacagcggct cgtagaactg caccaccaga
      481 ccttgccgaa aacgcttgaa caagttccgc accgtgtcgt cggtgggaaa gcgctggatt
      541 cccagcaggg catgcaaggc cttatccgtc cgcagcatcg ccgtgtgcgc aaaccgtcgc
      601 gcccccgcca ccaccgaaat cagaaacgcg gtgaaggtct cgcccggatc gatcgcattc
      661 ggagacttca aatgcaccgg caggtgctcg ctcacctgct gctgataccc aatcttcccc
      721 agaaacgcga caaaaacact cagccccccg aacggcgtca ccgcccgcgg cgtctgccgc
      781 aaaactactt tcttttctcc ccaaagcgcg gtaatttctc gttgcactgt tctgttcctc
      841 cgtagatttt gtctaaccaa cttcaatcta acggattaga acggaacagt gctccctaat
      901 ttcggaattc gagttgaagc cttcattacc gcctacgata aacatgcaaa gcctttcaag
      961 tggaggaagc gtgaggtaaa aggatcacag cttcgcaata ctatcgttaa tctatgcaat
     1021 taagcactag gcatcgaggg catgtttcca aacatccccc ccatgccacc gtggccgggg
     1081 tgttgcgccc acagccccgc ggtcatcgtc agcaccacca gcagcatcat cgtggccttt
     1141 ttcaaactac acataaacgc atccctccat tttggttggc cggggaattc tcataattcc
     1201 gccttagggc cagag
//
LOCUS       DATCBB010000380         1165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_336706, whole genome shotgun sequence.
ACCESSION   DATCBB010000380 DATCBB010000000
VERSION     DATCBB010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336706"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..975)
                     /locus_tag="VII37_04290"
     CDS             complement(283..975)
                     /locus_tag="VII37_04290"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HEY5068975.1"
                     /translation="MKNLRLLSTTAAVLLLGAGAVSAQGMKNDEAPARTPAAQQNAPA
                     EKVAPAMKPGEQKAGTKKAPDTIGQAPKASDSGKSQTMDKSAPVGAAADVKAGTKGDV
                     KSQATPKENEKSSENAGAKEAAPASAKPAGEQNSATTGQGAAAGSAKLTTEQRTKITT
                     IIKQHKVEPARLNVTVSVGTRVPDSVHFYPLPIEVFVVYPEWRGYDYILVGDQILVID
                     PRTHQIVAILEA"
BASE COUNT          219 a          326 c          322 g          298 t
ORIGIN      
        1 tcatacatcg aaaaaatgta gaaaataaaa ttcttcagcg cggcatccgg ccatacgtcc
       61 acagcattat cagttttcat gacgcgcatt ttttacaaaa ctatcctatg cgcgcggcag
      121 acgttggagt tttcacacga ccaagaccca aagccgacat tggaccggca gcaaacttgt
      181 ttccagcggc cttcacagct ggtctcctaa ttctatgttc aaagaagttg cagccggggc
      241 ttgcgccccg gccgcctacc gattgtcgtg atatgcgatg ctttacgctt cgagaatggc
      301 gacgatctgg tgcgtgcgcg ggtcgatgac gaggatctgg tcgccgacca ggatgtagtc
      361 gtaaccgcgc cactcaggat agaccacgaa cacctcaatc ggcagcggat agaagtgcac
      421 gctgtccgga acacgggtgc cgacagacac tgtaacattc agacgtgccg gttccacctt
      481 gtgctgcttg atgatggtgg tgatcttggt gcgttgttcg gtcgtaagct tcgcggagcc
      541 agccgctgcg ccttgacctg tggtggcgct gttttgctct ccggcgggct tggccgaagc
      601 gggagccgct tctttggcgc ccgcattttc gctactcttt tcgttttcct tgggcgtggc
      661 ttggcttttg acgtcgccct tagtgccggc cttgacgtct gctgcggcgc caaccggggc
      721 gctcttgtcc atcgtctggc tcttgccgga atcggatgct ttcggcgcct ggccgatcgt
      781 gtcaggggcc tttttcgtac cggccttctg ctcgcctggc ttcatggccg gggctacctt
      841 ttctgccgga gcgttctgct gcgcggcagg ggtgcgcgcc ggcgcctcgt cgttcttcat
      901 gccttgcgcc gaaacagcac cagcacccag cagcaatacc gccgccgtgg tggataatag
      961 tcgtaggttt ttcatggatg tctcctttgg acagtccgca aatgtttagt tttgcggctg
     1021 aaatccataa ccaacgcgtc tatcgccaaa aagttccaaa atgttttcat gatatcttat
     1081 acccaaactc ggaaatcttg actcatggtg cccaacgttg tgcggggatg atcgcattgt
     1141 aaatgcgaat cgaatcaagt gcttg
//
LOCUS       DATCBB010000381         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_238844, whole genome shotgun sequence.
ACCESSION   DATCBB010000381 DATCBB010000000
VERSION     DATCBB010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238844"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="def"
                     /locus_tag="VII37_04295"
     CDS             <1..522
                     /gene="def"
                     /locus_tag="VII37_04295"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5068976.1"
                     /translation="GPPRKIYSIVKYGDPILEKPTPAITEFGAELEELTEDMFASMYA
                     AQGVGLAAPQIGKNLRLTVVDVTGGKNPEAKIVLINPEIIHAEGEKREEEGCLSIPGF
                     RGYVVRPQFVTVKALNAKGEPFEIRGEDLLARAFCHEIDHLNGILFIQHLSMLKRDLI
                     RRKIKKLRKQGEW"
     gene            658..1596
                     /gene="fmt"
                     /locus_tag="VII37_04300"
     CDS             658..1596
                     /gene="fmt"
                     /locus_tag="VII37_04300"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5068977.1"
                     /translation="MRIVFCGTPAFAVPTLKAIMADPSHTVVAVITQPDRPRGRGQEI
                     SFSPVKEAALEAGIPVRQPVKIRAPEAREVLQNFEPDCFVIIAYGQIIPAGLLPLSRC
                     GWINLHASLLPKYRGAAPINWAIANGETKTGLTTMRIDAGMDTGDMLLQAEIEIVPKE
                     TAPELAARMADAGANLMIETLRQLDAGELDSKKQNDELATYAPLLKKEDGLIEWERKA
                     KEIFNRMRGFEPWPGAYTTFRGKTCHLRGEPATREQAPEVPGTLLLEDGELRVACGHA
                     TVLRVLTVKLEGRAEVSAKEFVNGARLAKEERFGNS"
     gene            1693..>2156
                     /locus_tag="VII37_04305"
     CDS             1693..>2156
                     /locus_tag="VII37_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068978.1"
                     /translation="MINFGPAEKHGCRSVGAKDVFGLWRVAMCGVVCAQLIVAPAGWA
                     ATENAATHRAAAAASTDASGSSAGAAAAAVDAKRVASDPVLRVMQAELSRATSELGKT
                     DQAPYYLSYTVYDQDYVVLVGAYGSLLTDAAAKRRQADVTMRVGSHELDNTHG"
BASE COUNT          494 a          467 c          727 g          468 t
ORIGIN      
        1 gggccaccac gaaagattta ttccatcgta aagtacggcg accccattct ggaaaagccc
       61 acacctgcga ttacggagtt tggggcagag ctggaagaat tgacggagga catgttcgct
      121 tcgatgtatg cggcgcaagg cgttgggttg gctgctccgc agattggcaa gaatttgcga
      181 ctgacggtgg tggatgtgac tggcgggaaa aatccggaag caaaaattgt actgatcaat
      241 ccggaaatta ttcatgcgga aggcgaaaag cgggaggaag agggttgtct ttctatcccg
      301 gggtttcgtg ggtatgtggt gcgaccgcaa tttgtgacgg tgaaagcgct aaatgccaag
      361 ggcgagccgt ttgagattcg cggtgaagat ttactggcgc gagcgttttg tcatgagatc
      421 gatcatttga acggcatcct gtttattcag catctgagca tgctgaagcg ggacctgatc
      481 cggcggaaaa tcaagaagct tagaaaacaa ggcgagtggt aacgctgtag ttaaggattg
      541 gccttcggtt cctcagaatt cctgcacgga tttcgcgatg ctgtcgtaac ttcaagtcag
      601 gttaaatacg tctcatcatt ttattggctt caaggttgtt ggccggaagg atgatagatg
      661 cggattgttt tctgcggaac gcctgccttt gccgtgccta cgctgaaggc cataatggcg
      721 gatccgagtc ataccgtggt ggcggtgatt acgcagccgg accggccgcg cgggcgcgga
      781 caggaaatat cgttttctcc agttaaagag gcggcgctgg aagcgggtat accggtgcgc
      841 cagcctgtga agattcgggc tccggaagcg agagaggtgc tgcagaattt tgagccggac
      901 tgctttgtga ttatcgctta cggacaaatc attccggcag gacttttgcc gctttcgagg
      961 tgtggctgga tcaacctgca tgcttccctg ctgcccaaat atcgtggagc ggcgccgatc
     1021 aattgggcga ttgcgaatgg cgagacgaaa accggattaa ctaccatgcg gatcgatgcg
     1081 gggatggata ccggggatat gttgctgcag gcggagattg agattgtacc gaaggagacg
     1141 gcaccagaat tggcagcgcg gatggcagat gccggggcta acttgatgat cgagacgctg
     1201 cgccagttag acgcgggaga gctggattca aagaagcaga atgacgagct ggcgacatat
     1261 gcgcctttgc tgaagaaaga agatggactg attgagtggg agcggaaggc gaaggagatt
     1321 tttaaccgca tgcgcgggtt cgagccttgg ccgggggcgt ataccacgtt tcgggggaag
     1381 acctgccacc ttagaggcga gcccgcaacg cgagaacagg cgccagaagt gccgggcacg
     1441 ctactactgg aagacggcga actgcgagtt gcttgtggac acgcaaccgt tttgcgtgtt
     1501 ttgaccgtca aactggaagg acgcgcggaa gtttcggcga aggaatttgt gaacggggca
     1561 cggttagcca aagaagaacg ctttggtaac agctaaattg cgcgtgagtg agcgctgagg
     1621 catactcggt ttctccgctg ttttcgtgga tcaggcacgt cgaatcgtca tatcgatcgg
     1681 agttcacagt taatgattaa ttttggccca gcagaaaagc atggatgcag gagtgtagga
     1741 gcgaaggacg tctttgggtt gtggcgtgtg gcgatgtgcg gggtggtttg cgcgcaactc
     1801 atagtggcac ctgctggttg ggcggccacg gaaaatgcgg cgacacatcg cgcagcagcg
     1861 gctgcatcga cggatgcttc gggtagttcc gcgggagctg cggcggccgc ggtagacgcg
     1921 aagagagtcg ctagcgatcc ggtgctgaga gtgatgcagg cggagctttc gcgtgcaaca
     1981 agtgagttgg ggaagactga ccaagcgcct tactacctaa gctacacggt gtatgaccag
     2041 gattatgtgg tgctggtggg cgcgtatggg agcttgctta cggatgcggc ggcgaagcgg
     2101 cggcaggcgg acgtaacgat gcgggtggga tcgcatgagc tagacaatac tcacgg
//
LOCUS       DATCBB010000382         1062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_304826, whole genome shotgun sequence.
ACCESSION   DATCBB010000382 DATCBB010000000
VERSION     DATCBB010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304826"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /gene="nrfD"
                     /locus_tag="VII37_04310"
     CDS             <1..601
                     /gene="nrfD"
                     /locus_tag="VII37_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY5068979.1"
                     /translation="TTIFPPYFVAGAIYSGFAMVLTIAIPLRKFYHFEDFITMRHLEN
                     MAKVMLATGLIVAYGYFFEFFMSLYSGQKFDVFLVQQRLHGPYSHYYFALILCNILTP
                     QLLWIRAVRTNPTVLFLMSIVVNIGMWLERFVIVVISLTRDFMPSAWGRYSPTVWDWA
                     TFIGTLGLFTTLIFLFVRVLPAISIAEMRELVAHEEEHE"
     gene            601..>1062
                     /locus_tag="VII37_04315"
     CDS             601..>1062
                     /locus_tag="VII37_04315"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY5068980.1"
                     /translation="MEHRSHLYGVMGEFETAKHFLQAVAQVRDAGYRNMDAYAPFPVE
                     GLSEAMGLRRSWVPLVTLLGGLGGGLTGFGFQYWVNVFAYPMNIAGRPLNSWPAFIPV
                     TFELTILGAATFAVFGMLAMNKLPHPYHPVFNVQRFARASSDRFFICIEATD"
BASE COUNT          189 a          306 c          269 g          298 t
ORIGIN      
        1 caccactatt tttccgccgt acttcgtggc cggcgcgatt tattccggct tcgctatggt
       61 gctgaccatc gccattccac ttcgcaagtt ttatcacttc gaagatttca tcaccatgcg
      121 ccatctcgaa aatatggcca aggtgatgct cgccaccggc ttgatcgtgg cgtacggata
      181 tttctttgaa ttttttatgt cgctctacag cggccagaaa ttcgatgtat ttctcgtaca
      241 acagcggctg catgggccgt attcgcacta ctacttcgcc ttaatccttt gcaatatttt
      301 gaccccgcag ctgttatgga tacgcgccgt gcgcaccaac ccaacagtgc tcttccttat
      361 gtccatcgtc gtgaatatcg gcatgtggct ggagcgcttc gtgatcgtgg tgattagtct
      421 tacgcgcgat ttcatgcctt ccgcgtgggg ccgctactcg ccgacagtgt gggattgggc
      481 cacctttatc ggcacgctcg gcctcttcac tactttgatt ttcttgtttg tgcgcgtgct
      541 tccggccatt tccatcgccg aaatgcgcga gctggtcgcc catgaggagg agcacgagta
      601 atggaacacc gctcccacct ttacggcgta atgggcgagt tcgaaactgc gaagcatttt
      661 ctccaggcgg tagctcaagt tcgtgatgcg ggctaccgca acatggacgc ctacgcgcct
      721 tttcccgtgg aaggtttgtc ggaagctatg ggccttcgtc gctcttgggt cccccttgta
      781 acgctgctag gcggcctcgg gggcggctta accggttttg gattccagta ttgggtcaac
      841 gttttcgctt accccatgaa tattgccggg cgcccgctga atagctggcc cgcctttatt
      901 cccgtcacct tcgaattaac aattcttggt gccgccactt ttgcagtgtt cggaatgctg
      961 gcgatgaaca agctgccgca tccctaccat ccggttttca atgtgcagcg atttgcacgc
     1021 gccagctccg atcgattctt tatctgcatc gaggcgaccg at
//
LOCUS       DATCBB010000383         1576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_304832, whole genome shotgun sequence.
ACCESSION   DATCBB010000383 DATCBB010000000
VERSION     DATCBB010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304832"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VII37_04320"
     CDS             <1..1439
                     /locus_tag="VII37_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068981.1"
                     /translation="GVKPVGLWPSEGSVSDQTLAIASEEGFKWFGTDEGVLGRTLNVG
                     FFRDGTGIPANAERLYKPWRVQTPSGGITGLFRDHHLSDLIGFVYSRMDSKGAAADLH
                     GRLRYLGERVQSAQPLTICIFLDGENAWEYYPGNGREFLREFYGRVASDQDFRALKAS
                     EAIAAAGEVPVTAGIFPASWINANFDVWIGHSEDVSAWELLWDAREIYAKAVESSKEG
                     HADAPGVTALSTAYESILAAEGSDWCWWFGPEHSTANDAEFDALFRKHLTGVYLALGR
                     VPPAELAKPIKRKPEHALELAPTGFVNVRVDGRDSSYFEWLGAGLYSPERRGGSMHGR
                     IFYLHELRYGFEEDRFCVRVDLFPEVLAELEDTEFRVSIVGAEELTVVAKIGRGHLRE
                     FAVEQKNVCLLNPKDIAEVAFDRILEVSIRREAVDIAGKTSLRLGVALWHCGLPVDVL
                     PASGFLDVSFGEEHAAWPIETVEADSKK"
BASE COUNT          337 a          326 c          526 g          387 t
ORIGIN      
        1 ttggagtgaa gccggtggga ttgtggccat cagagggatc ggtctccgac caaactttgg
       61 caattgcatc ggaagaagga tttaaatggt ttggcaccga tgaaggagtg cttgggcgca
      121 ccttgaatgt aggatttttt cgagatggga caggcattcc tgcgaacgcg gaaaggttat
      181 acaagccatg gcgagtacaa acgccaagtg gcggcatcac gggattattt cgcgaccatc
      241 atttatcgga tttgatcgga tttgtgtata gccgcatgga ttcaaagggt gcggccgcag
      301 atctgcacgg acggttgcga tatctgggcg aacgagtgca aagcgcgcag ccgctgacca
      361 tttgtatttt tctggatggc gaaaatgcgt gggaatatta ccccgggaac ggccgcgaat
      421 ttttgcgtga attttacgga cgagtggcga gcgatcagga tttccgcgcc ttgaaagcct
      481 cggaagcgat tgccgcggcg ggagaagtgc ctgtcactgc gggaattttc ccggcttcct
      541 ggattaatgc gaacttcgat gtgtggattg gtcacagcga ggacgtttcg gcgtgggaat
      601 tattgtggga tgcgcgcgag atctacgcga aggcagtgga gtccagcaaa gagggtcacg
      661 cggacgctcc tggagtcacg gcgctaagca ctgcgtacga atcgatcctg gctgcggaag
      721 gcagcgattg gtgttggtgg tttgggccgg agcacagtac cgcgaatgat gcggagttcg
      781 acgcgttatt tcgcaagcat ttgacgggcg tttacctcgc gctcgggcgc gtgccgcctg
      841 cagaattggc caagcccatc aagcgtaaac cggaacatgc gttggagctg gcgcccacag
      901 gatttgtaaa cgtgcgggtg gacgggcgcg acagttcgta ttttgagtgg ctgggcgcgg
      961 gtttgtattc tccggagcgg cgcggcggat cgatgcatgg gcgcattttt tatttgcatg
     1021 aattgcgtta tggatttgag gaagatcgtt tctgcgtgcg cgtagatctt tttcccgagg
     1081 tgcttgcgga attggaagat acggaatttc gcgtcagcat cgtgggcgcg gaagaattga
     1141 cggtagtggc aaagattggg cgcggacatt tgcgagagtt tgcggtggaa caaaaaaatg
     1201 tttgcctgct gaatccgaaa gatattgcgg aagtggcgtt tgaccgcatc ctggaagtgt
     1261 ccattcgacg agaagcggtt gatattgctg ggaagactag cttgcggctg ggagttgcgc
     1321 tgtggcactg cgggttgccg gtggacgtgt tgcctgcgtc gggatttttg gacgtgagtt
     1381 tcggcgaaga gcatgccgca tggccgatag agacggtcga agcagattca aagaaataat
     1441 ttgcgcgttg actcggctgc aggcagcaga gtcttgtttg tcattttaca atcgtgaatg
     1501 tttgattttt gagaaatgtt ctggcgtcgt cttcgttgaa aaaaaccagg cggatatgct
     1561 ctagcggtga tccggc
//
LOCUS       DATCBB010000384         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_434568, whole genome shotgun sequence.
ACCESSION   DATCBB010000384 DATCBB010000000
VERSION     DATCBB010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434568"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VII37_04325"
     CDS             complement(<1..371)
                     /locus_tag="VII37_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068982.1"
                     /translation="MSATLDTIRQSLTATAKVDFKALEVSGNVRVELHPNLEVQSVKS
                     ADGKLLNFERDRQNPLFLQVTVPSPVGAGGHLSLTFAYTGLLANEENSPVPGVRAAVI
                     NHDFAYLLLPSRWFPLTDYPS"
     gene            complement(588..1751)
                     /gene="lpxB"
                     /locus_tag="VII37_04330"
     CDS             complement(588..1751)
                     /gene="lpxB"
                     /locus_tag="VII37_04330"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY5068983.1"
                     /translation="MTATPILLSAGEASGDMYAARLATALKERADVELFGMGGPQMRA
                     AGVEIIVDYAEVSVLGITEVLKKLPALRRAMGRLVDEAQKRRSPLAILTDFPGFHLRL
                     ARKLSPLGIRNIYYICPQFWAWRPWRANLVRRRFAQALCIFPFEETFYRDAGVAVEFI
                     GHPLVGNVNASMSREEFCAENGLSTDKPIVTILPGSRRGEIEHHAAILAQALTQMRLA
                     IPGIQPVVALAPGVNRADLEACFSSDSAVRFVTGATYNALAAADLAVISSGTATVEAA
                     LLGKPMLVIYRLSPLTAKLAKPLVRTKFFSMVNLIAGRAVVPELIQDDFTPERVASEA
                     ISLLSASKESVAKLEDIRRGLTEVTRLLGPPGAVERAADAIIRQLRLAQSNAK"
     gene            complement(1860..>2528)
                     /locus_tag="VII37_04335"
     CDS             complement(1860..>2528)
                     /locus_tag="VII37_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5068984.1"
                     /translation="KTTLISLLLRFYDIQRGQILLDGIDIRMIELQDLRRQFGIVLQD
                     PFLFSGTIESNVRLGTAGIDRSTVEHAVNEVGLGDFIRALPDGVDTSVNERGSTLSVG
                     QRQLINFARALAHNPRFLILDEATSSVDTKTELLIREALDRLLSGRTALVIAHRLSTI
                     QHADCILVFHKGRLREQGAHQELLAQRGIYYRLYQLQYKEQELHLPVDVSARPEPSSL
                     QAND"
BASE COUNT          587 a          717 c          672 g          552 t
ORIGIN      
        1 ttcgagggat agtccgttag cggaaaccat cgcgagggca gcagcagata agcgaaatca
       61 tgattgatca ccgcagcgcg aactcctggc accgggctgt tttcttcatt ggccaacaat
      121 cccgtatagg cgaacgtaag cgacaaatga ccacccgcgc ccaccggcga tggcacggtc
      181 acctgtaaaa atagaggatt ctgccgatca cgctcaaagt tcagcaactt gccgtccgca
      241 gattttaccg attgcacttc gaggttcgga tgcaactcca cgcggacatt tcctgaaact
      301 tccagcgctt tgaaatcaac tttggcggta gcggtaagag attgacggat ggtgtccagc
      361 gtggcggaca catcataatt ttctgcaata aacgctattg gccgcgggcc ctgcgctctc
      421 aaccacccag ccgcgaacaa tgccagcgtc aacgctccca agagcgctag tggccttaaa
      481 gctttcttac ggagggtcat cggacccctt tcacggtttt gcattggaat cggcacagcg
      541 acttgaacgc cgcagctcgc agctacctct ataggatacc aaacccgtca ctttgcgttg
      601 ctttgtgcca gccgcagctg ccttatgatg gcgtcggcgg cccgttctac ggctcccgga
      661 ggccctaaca atcgtgttac ttccgtcagg cctcgtcgga tgtcttccag tttagcgacg
      721 ctttctttgg atgccgacag cagcgatatg gcttcagaag cgactcgttc tggagtgaaa
      781 tcatcctgga tcaactctgg cacaactgcg cgtccagcaa tcaggttaac catgctgaaa
      841 aatttggtgc gcactagcgg tttggcgagc ttggcggtca acggtgacag cctgtaaatc
      901 acaagcatcg gctttccgag taacgccgcc tcaacggtgg cggtcccgct cgagatgacc
      961 gctagatctg cagccgccaa cgcattgtag gtggcgcctg ttacgaacct cacggcactg
     1021 tccgaactaa agcaggcctc tagatctgct cgattcaccc cgggagctag ggcgacgacc
     1081 ggctgaattc ccggaattgc aagtcgcatt tgcgtcagcg cttgagccag aatcgcagcg
     1141 tggtgttcaa tctctcctcg gcggctgccc ggcaaaatgg tgacgatcgg tttgtcagtg
     1201 ctcagtccat tttcggcaca aaactcctcg cgcgacatgc tcgcgttcac attccccacc
     1261 aacggatgcc cgataaattc gactgccaca cccgcgtcgc gataaaacgt ttcttcaaaa
     1321 ggaaagatac ataaggcttg cgcaaacctg cgccgtacca ggttcgcgcg ccacggccgc
     1381 caggcccaga attgcgggca aatataataa atgttccgaa tgcccagcgg cgagagcttt
     1441 cgtgccagcc gtaaatgaaa tccgggaaag tcagtgagaa ttgcgagtgg cgagcggcgc
     1501 ttttgcgctt cgtcaaccaa gcgacccatc gctcgtcgca aagccggcaa ttttttcagc
     1561 acttccgtaa tgccaagtac cgacacctcc gcgtaatcca caatgatttc gacaccggct
     1621 gcgcgcattt gcggtccacc catgccaaac agctcaacat ctgcccgctc ttttagagcc
     1681 gtggccagcc gagctgcata catatcgcca gaagcctcgc ctgcggaaag caaaatgggt
     1741 gttgcggtca tgaacaggca acgaaatttg ttttgtgagg ttttttctta agaagtacga
     1801 aaagcggaaa cacgcaaatc tgttgctggc ccgagtttat cgccaggaaa tcatgatgct
     1861 cagtcattag cctggagtga tgaaggttct ggtcgggcgc tgacgtcgac cggcaagtgt
     1921 aattcctgtt ccttatattg cagctggtac agccggtaat aaatcccgcg ttgtgccagc
     1981 agttcctggt gcgcgccttg ctcgcgcaag cggcctttgt ggaataccaa aatgcaatcg
     2041 gcgtgctgga tggtgctcag gcggtgagcg atgaccagcg cagtgcgacc cgagagcaag
     2101 cggtccagcg cttcgcgaat gagcaattcc gtcttggtat ccacgctgga ggtagcttcg
     2161 tcgagaatga ggaagcgcgg gttgtgcgcc aacgctcggg caaaattaat gagttgtcgt
     2221 tgcccgaccg acaaagtcga gccgcgctcg ttcaccgaag tatctacgcc atccggcagt
     2281 gcgcgaataa aatcgcccag gcccacttcg tttacggcgt gctctactgt ggagcgatcg
     2341 atccccgcag ttcccagtcg cacattcgat tcgatggttc cggaaaagag aaaaggatcc
     2401 tgtagcacga tgccgaattg ccgacgcaga tcctgcaatt cgatcatgcg aatatcaatg
     2461 ccatccagca aaatttgtcc gcgctgaata tcgtaaaatc gcagcagcag ggaaatcagg
     2521 gtagtctt
//
LOCUS       DATCBB010000385         3844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_175094, whole genome shotgun sequence.
ACCESSION   DATCBB010000385 DATCBB010000000
VERSION     DATCBB010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175094"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VII37_04340"
     CDS             <1..427
                     /locus_tag="VII37_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068985.1"
                     /translation="PQRGNNAGGAGGDQGQGRASYRDQQGHPEAPHVHAQNDQWVGHD
                     AGRNDARYHQDHVWEHGHFGGEIGRNHIYRIEGGGRDRFWFSGFYWGVAPYDYDYTND
                     WLWNSDDIVLYDDPDHPGWYLAYNTRLGTYVHVQYLGNQ"
     gene            673..1689
                     /gene="uppS"
                     /locus_tag="VII37_04345"
     CDS             673..1689
                     /gene="uppS"
                     /locus_tag="VII37_04345"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY5068986.1"
                     /translation="MPHTKQLLPSPIGAPKNFGPLSVEESELLQQLDLSRMPRHVAVI
                     MDGNGRWAQRRHLPRIAGHRYGTKTARTTIETCARLNIEALTLYAFSVENWRRPKVET
                     DFLMQLLREYIRKEMPLIQRNNIRMRFLGRIDELPLGVQKDVREATEQTAANTGMCLC
                     IAFNYGGRAEIVDAMNAILAQRAGYGTNGHGASGHVAPNRNGDVSAKARIDADSNPKK
                     PEYSNAADQHECGNGNGHFQRITEDEVSSYLYTEGLPDPDLLIRTSGEMRVSNFLLWQ
                     IAYAEMYVSETLWPDFNRARLLEALVEFQKRDRRYGGLQQQPNSSSNSEDASLDDSPR
                     AAGK"
     gene            1792..2652
                     /locus_tag="VII37_04350"
     CDS             1792..2652
                     /locus_tag="VII37_04350"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY5068987.1"
                     /translation="MATAAVLIPVVVGLVLWGSTIVVALVMALVMLLALFEYFALGDA
                     MGHRAYRFWTATCALIILYLQWLTTAVRWGEFGAIIYPARLVWFLHRFFPRAEDGIFL
                     FVLGIAFITLATKRPIVEALPSAGISSSGLLLVAFPLTYAIRLHAQGREGPRLLLFVL
                     VLIWASDSAAYFAGRAFGKHPFAPHLSPKKTWEGSIAGFASSIVVAFAFMRWLSVPIA
                     ILLGAAAVGNVAGQIGDLLESAYKRSAGVKDSGALLPGHGGMLDRIDALILAIPVVWY
                     YFVLLYPPTV"
     gene            2720..>3844
                     /gene="dxr"
                     /locus_tag="VII37_04355"
     CDS             2720..>3844
                     /gene="dxr"
                     /locus_tag="VII37_04355"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY5068988.1"
                     /translation="MNHLAILGSTGSIGRQCLSVVEESLPGQFVVCGLAAGSNLEELV
                     KQIERHRPNVVSVADGKKADELAERLRAKKIGPLPSIHHGREGMLTIATLARAEIVVS
                     AAVGVVGLEATYEAVKLGKTVALSNKEVLVAAGELVMAAAKKTGRELLPVDSEHNAVH
                     QCLRGGKASEVRRLVLTASGGPFRKTPLSNFDSITPEQALAHPNWRMGNRITIDSATM
                     MNKGFEIIEARWLFGMRPDQIDVVIHPQSTVHSFVEFIDGSILAQLGPTDMRMPIQYA
                     LTYPERHASNQIALDWKKLKRLDFASASTRRFPCLKLAREAMKKGGALPCALNAADEI
                     AVAAFLERRLSFPGIAEVIESVLARTPRVKFENMSDVLAAD"
BASE COUNT          831 a         1164 c         1007 g          842 t
ORIGIN      
        1 gccgcagcgc ggtaacaacg ccggcggagc tggtggcgac caaggccaag ggcgcgcatc
       61 gtatcgcgac cagcaaggcc atccagaagc acctcacgtg catgctcaaa acgatcaatg
      121 ggtcggccat gacgcgggcc gtaacgacgc tcgttaccac caggaccacg tttgggaaca
      181 cggacacttc ggcggcgaaa ttggccgcaa tcacatttat cgcatcgaag gcgggggccg
      241 agaccgcttc tggttcagcg gcttttactg gggcgtcgcg ccatatgact acgactacac
      301 caacgattgg ctgtggaaca gcgacgacat cgtgttgtac gacgacccag atcacccagg
      361 ctggtatctc gcctataaca ctcgactcgg aacctacgtc cacgtgcagt acctcggcaa
      421 ccagtaagtg ttgtctagct gcctcgctgt ctgagccaca tcggaaaacc ccatgcgcga
      481 agattcgcgt acggggtttt cttgtcttag gcgaagcgga tcgcggcctg tcggtttctt
      541 gtaattttgc ctcgctaacc gtgatgcgaa acaatcccga tactctcgcg acacatcgtt
      601 tgcatccctg caatccgtat gttaactttg cgcggtaaag ggaattcgcc caagaacctc
      661 gcgacggtcg ccgtgccaca caccaaacaa ctcttgcctt cgcctatcgg cgctccaaag
      721 aacttcggtc ccttgagcgt ggaagaatcc gagctgcttc agcagctcga tctgtcgcgt
      781 atgccccgtc acgtggcggt gatcatggat ggcaatggcc gctgggcgca gcgccgtcat
      841 cttccgcgca tcgccggcca ccgctacggc accaagactg cccgcaccac cattgaaact
      901 tgcgcgcgac taaacatcga agctctaacc ctctatgcct tttccgtcga gaactggcgg
      961 cgccccaaag tcgaaactga tttcctgatg cagctgttgc gcgaatatat ccgcaaagaa
     1021 atgccgctca tccagcgcaa taacatccgc atgcgtttcc tcggccgcat cgacgaactt
     1081 cccctcggag tgcaaaaaga tgtacgcgaa gccaccgagc agaccgctgc aaacaccggc
     1141 atgtgtctgt gcatcgcctt caactacggt ggccgcgccg aaatagtgga tgccatgaac
     1201 gccatcctcg cgcaacgtgc cggctacgga acgaacggcc acggggccag tggccacgtt
     1261 gcaccgaacc gcaacggtga tgtatccgcg aaagcgcgaa ttgatgccga ctcaaatccg
     1321 aaaaaaccag aatatagcaa cgctgcggat caacatgagt gcggcaatgg caacggccac
     1381 tttcagcgca tcaccgaaga tgaagtttcc agttatctat acactgaagg cctgccagac
     1441 ccggatttgc tcatccgcac cagcggagaa atgcgcgtaa gcaatttcct actatggcaa
     1501 attgcctacg ccgaaatgta cgtcagcgaa acactatggc ccgacttcaa ccgcgcgcgc
     1561 ctgttggaag ccttggtaga gttccaaaaa cgtgatcgcc gctacggcgg cctgcaacaa
     1621 caaccaaatt cctcatccaa ctccgaggac gcctcgttag acgattcccc ccgcgccgcc
     1681 ggcaaataat ctccgatcac attccacgat acgaagcatc ttcgactctc aatatgatgt
     1741 aatgttccgg cacttaccac aggggactcg tctttcatga cttggaaaag agtggctacc
     1801 gcggcggtct taattcccgt ggttgttggc ctcgtacttt ggggttctac catcgttgtg
     1861 gcgctggtaa tggcgctggt gatgctcctc gcgctatttg aatatttcgc gttgggcgac
     1921 gccatgggtc atcgcgccta tcgtttctgg accgctactt gcgcgctgat tattttgtat
     1981 ctgcagtggc tgaccaccgc agtccgatgg ggagaattcg gtgccatcat ttatccagcc
     2041 cgtttggtct ggtttcttca tcgctttttc ccacgggctg aagacggcat ctttctcttt
     2101 gtgttgggaa ttgcgttcat cacactagcc accaaacgac ctatcgttga agcgttaccc
     2161 tccgccggta tcagttccag cggcctgttg ctcgtcgcat ttccacttac ttacgcaatt
     2221 cgcctgcatg cgcaaggcag ggaagggccg cgtctgttgc tttttgtgct ggtgctgatc
     2281 tgggccagcg attccgctgc atacttcgca ggccgcgcgt tcggcaaaca tccctttgcg
     2341 ccgcatctta gccccaagaa aacctgggaa ggttcgatcg ccggatttgc gagttctatt
     2401 gttgtggctt ttgcttttat gcgctggctt tcggtcccaa tcgcgatttt gctcggcgct
     2461 gccgctgtgg gcaacgttgc gggtcaaatt ggcgatttgc tcgaatctgc ctacaaacgc
     2521 agcgcaggag tgaaagattc cggcgcgctc ttgcccggtc acggaggcat gctggatcgc
     2581 atcgacgcgc ttattctggc catccccgta gtctggtact attttgtttt gctgtacccg
     2641 cccaccgtct aggcgcacat tccttgcgaa acgcgttgcg atgggtatgt ttgacgccca
     2701 cacgtcggaa agagactgcg tgaatcatct agctatcctc ggttccaccg gctccatcgg
     2761 ccgccaatgt ctctccgtcg tggaggaatc gcttcccggc caattcgtcg tgtgtgggct
     2821 ggccgctgga tcgaacctcg aagagctcgt caagcaaatc gaacggcacc ggccaaacgt
     2881 tgtttctgta gccgacggaa agaaagccga cgagctcgcc gaacgcttgc gcgccaaaaa
     2941 gatcgggccg cttcccagca ttcatcacgg acgcgaaggc atgcttacaa tcgcgacgct
     3001 tgcgcgagcc gaaattgtcg tctcagcagc cgttggcgta gtcggccttg aagccactta
     3061 tgaagctgta aaactcggca aaaccgttgc gctttccaat aaagaagttc tggtggctgc
     3121 cggtgaacta gtcatggccg ccgccaaaaa aacaggccgc gaactccttc ccgtcgacag
     3181 cgaacacaac gcagtacacc aatgccttcg aggcggtaaa gcctcggaag ttcgccggct
     3241 cgtactcacc gcatcaggcg gcccattccg caaaacgcca ctttcaaact tcgactccat
     3301 cacgccggag caagcgttgg cccatcccaa ctggcgcatg ggcaatcgca tcaccatcga
     3361 ttccgccacc atgatgaata aaggcttcga gattatcgaa gcccgctggc ttttcggcat
     3421 gcgccctgat cagatcgacg tagtgatcca tccgcaatcc acagtgcatt cttttgtcga
     3481 atttatcgac ggcagcatcc tggcccagtt gggccccacc gacatgcgta tgcccattca
     3541 gtacgcgtta acctatcccg aaagacacgc atctaaccag atagcgctgg actggaagaa
     3601 actaaagcgt ttggatttcg cctcggccag cacccgacgt ttcccctgtt tgaaattagc
     3661 gcgggaagcc atgaaaaaag gcggcgcatt gccttgcgcg ttaaacgccg ccgacgaaat
     3721 cgctgtggct gcttttctgg agcgccggct ttcgttccct ggcatcgctg aagtgatcga
     3781 aagcgtgctg gcgcgcacgc cgcgcgtgaa gtttgaaaac atgtccgacg tgctcgccgc
     3841 ggac
//
LOCUS       DATCBB010000386         1224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_142813, whole genome shotgun sequence.
ACCESSION   DATCBB010000386 DATCBB010000000
VERSION     DATCBB010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142813"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..627)
                     /locus_tag="VII37_04360"
     CDS             complement(265..627)
                     /locus_tag="VII37_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY5068989.1"
                     /translation="MDFTSSRWLPVVLLTLSNIFMTVAWYGNLNPKFKNSPLWLVILA
                     SWGLAFFEYVFMIPANRWGSSVYSVTQLKIIQEVITLSVFCVFAILFLGEKIRWNYIA
                     AFVCILAAVAFAFWPKPQ"
BASE COUNT          317 a          336 c          315 g          256 t
ORIGIN      
        1 gtgcaaaagc gatgaacgga aaaatcgccg acaggaaagc gaacgcggcg atggcgggaa
       61 attgtctcat caagcgtggc caattaaaca gaacgcttcg cgactgccaa gagagtttta
      121 accgccagaa cacgcccgga cgaagaaaaa gaaattccac cacggattaa cccagataaa
      181 cccggataag attttgattt acgccagctc cagttgttga atccgtgtcc atccgtgatt
      241 aaacttttgt gtttgccttg gcggtcattg cggcttcggc cagaacgcaa acgccaccgc
      301 cgcaaggata catacgaacg ccgcgatgta attccaccgg attttttcgc cgagaaataa
      361 aattgcaaac acgcaaaaaa ccgaaagcgt gatgacctcc tggatgattt tcaactgcgt
      421 gacggaatat accgaactgc cccaacggtt ggccgggatc atgaaaacat attcaaagaa
      481 cgcgaggccc cagcttgcga ggatgaccag ccagagcggc gaatttttga atttcgggtt
      541 caggttgcca taccatgcga ccgtcatgaa aatgttcgag agggtcagca gcacgaccgg
      601 tagccagcgg gatgaagtaa aatccatgtg ccaaaaatga attcatcggc caccggtgtc
      661 aaattggaat atgtggtttt ctgccgcacc gccggcgctg aagccgaccg ctcttgccgc
      721 cgacgtgaac atcaggcgtt ggaaattccc aggctgtgtt tgcaaaatcg aattgtaacg
      781 gaaatgtccg atggtcgcgg agcgcctgga ctgcggcgtc ccgcgcctgc gggagccgcg
      841 gttggaacgc acggaaacca tccgtttgcc cgaagttctc ttccgcacga aagcgccgct
      901 caagcgggcg cagtccagat gcttcgcgat tttgctggtg ttatttattt tgaaaacacg
      961 ccatagcagc gttccatatt cttcgccacc tgcttcatca cttgctcatg cgccggtgca
     1021 gcaaaacttt tcacaacttt ccgttcgaca acgcgacgcg gcgttcacaa actggtttca
     1081 tgcctgacac acctgctgcc tatcaaaagc tgctcaagcg cactcgtgaa atcgcgctcc
     1141 tcgatagcgc caacagcctg ttgtcctggg acaaggaaac ttatatgcca gcccaggcgc
     1201 tcgaccatcg cgccgggcag atgg
//
LOCUS       DATCBB010000387         1212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_77331, whole genome shotgun sequence.
ACCESSION   DATCBB010000387 DATCBB010000000
VERSION     DATCBB010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77331"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VII37_04365"
     CDS             complement(<1..662)
                     /locus_tag="VII37_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068990.1"
                     /translation="MSDKYLRENQWWISPYNFVPEVLSNRELPARVSIHDATLRDGEQ
                     TPGVVFSVADKIAIAEKLDEVGVERIEAGMPAVSDQDFEAIKAISKMGLKSQIFTFAR
                     AMTADIDKALECGAHGVIVEIPIGYPKLKYQFKWTWEDVLRKSVDVINYAKKRGLYVC
                     YFPYDTTRAREEDLRNLFTRLMLDAPPDSIGVVDTMGCALPDAIKYLVRLVKSLTKLP
                     VEV"
     gene            complement(666..1067)
                     /locus_tag="VII37_04370"
     CDS             complement(666..1067)
                     /locus_tag="VII37_04370"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY5068991.1"
                     /translation="MKTAAAVVVLAILGGAPVWAHHAFAAEFDAKKPVKLRGTVTKME
                     WINPHAWIHVDVKKADGTVDEWMVEAGTPNTLLRRGFTRDSLKPGTEIIVDGYQSKDG
                     SLRANGRDVTLPDGKTLFLGSSAPDGAEDKK"
BASE COUNT          246 a          430 c          271 g          265 t
ORIGIN      
        1 acttccaccg gcagcttcgt cagggacttc accagccgga ctaaatattt aatagcatcc
       61 ggcagcgcgc agcccatggt atccaccacg ccaatcgaat ccggaggcgc gtccagcatc
      121 agacgggtga atagattccg caaatcttcc tcacgcgccc gcgtcgtgtc gtaaggaaag
      181 tagcagacgt acaatccgcg ctttttcgcg tagttgatca cgtccacgct cttgcgcagc
      241 acgtcttccc aagtccactt gaactgatac tttagcttgg ggtagccaat cgggatctcg
      301 acaatcaccc catgtgcccc gcattcgagc gccttgtcga tatccgccgt catcgcccgc
      361 gcaaaagtga agatctgcga cttcaacccc attttcgaga ttgccttgat ggcctcaaag
      421 tcctgatccg acaccgccgg catcccggct tcgatgcgct ccacccccac ttcgtccagc
      481 ttctcggcaa tcgcgatctt atcggccacg ctaaacacca cacccggcgt ctgctccccg
      541 tcgcgcaagg tagcgtcgtg aatgctcacc cgcgccggca gctcgcgatt actcagcact
      601 tcaggtacaa agttataagg actgatccac cactgattct ctcgaagata tttatcgctc
      661 atactctatt ttttatcttc cgcgccgtcc ggcgccgacg agcccagaaa cagcgtcttt
      721 ccgtcgggca gcgtaacgtc acgcccattg gcccgcagcg acccatcttt tgactgatac
      781 ccgtccacga taatttccgt tcccggcttc agcgaatccc gcgtgaaccc gcgccgcaac
      841 agcgtattcg gcgtccccgc ctccaccatc cactcatcca cggtgccgtc ggcctttttc
      901 acatcgacgt ggatccaggc atgcggatta atccactcca tcttggtcac cgtgccacgc
      961 agcttgaccg gtttcttagc gtcaaactcc gcggcaaaag catgatgcgc ccacaccggc
     1021 gctccgccca aaatcgccag cacaaccaca gcagcagcgg ttttcatttc acttctcccc
     1081 tcttctcccg cgcgcgagca cccgtcagga agtgcaccat atcgtaatta tcttcgtggc
     1141 acgcgtactc atacatctgc tcgcccggat cgatccggtt ccacggaatt cgcgccgtcc
     1201 acggccgcgt cc
//
LOCUS       DATCBB010000388         1538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_110903, whole genome shotgun sequence.
ACCESSION   DATCBB010000388 DATCBB010000000
VERSION     DATCBB010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110903"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(523..>1538)
                     /locus_tag="VII37_04375"
     CDS             complement(523..>1538)
                     /locus_tag="VII37_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY5068992.1"
                     /translation="LQAHSRHKIRTDHLQTIAPRLVAPQHQGRCFEQLLRAQLLQMLY
                     SVRSERLLMEEMDYNILFRWFVGLNLDDRVWDATVFSKNRDRLLEAEVAKEFLARVVA
                     QAREKGWTSDEHFSVDGTLLEAWASGKSFQPKDKKSSPPPDDPGNPTVNFRGEERSNR
                     THESKTDPDALLARKSAGKEAKLSYSGNLLVENRNGLIMSSLVWEATGTAERDAAMAM
                     LQDVPGSERVTVGGDKGFDTAEFVRECRNMHVTPHVAQNLTRRGGSAIDGRTTQHASY
                     RISQKKRKRIEECFGWLKTIALLRKVRHRGTLKVDWMFTFACAAYNLVRMRNLMAATV
                     PEQ"
BASE COUNT          290 a          505 c          390 g          353 t
ORIGIN      
        1 agacgacgcg ccgtgatcct caaccgcggg aagccgctgg attgctccta ccggcctgaa
       61 gcaacaacgt aggcttgacg agatccccga tttgttcgcg gaccctctgc gccaggcgag
      121 acacgtcctc tctgccctcc acttcgatcc ttaaactctg taattcaacc ttgatcttga
      181 acttcgacat attaagctcc tatccgacca taagcgcctt aattgccagc atttcgcgta
      241 taatgattct cctggccaag ccacgagcag gggtcgaacc tgcatctccg gggcgtgaac
      301 gcgggcgggc tccacgggaa atggcattcc cgcccacgct cacttcgaaa ccaccggcgt
      361 cttatccgtt tagcaggctg cggaaaaaag tgtgttcagc gagcgatttc tcctctcgtt
      421 gcgtcgtttg ttagccctga gaggattttc actggaatgt gtcgtgttgt cggcgccccg
      481 agtgtgcgtt tcagcaccgt gcgcagacac tacgaccctg actcactgct cgggaaccgt
      541 agcggccatc aggtttcgca tgcgtaccag gttgtaggcc gcgcaggcga aggtaaacat
      601 ccaatccact ttcaacgttc cgcgatgccg caccttgcgc agtaacgcaa tcgtcttcaa
      661 ccagccaaag cactcttcga tgcgcttcct tttcttctgg ctgatgcggt agctcgcgtg
      721 ttgcgtggtg cgcccatcga tggcgctgcc gcctcgtcgc gtcaggtttt gcgccacgtg
      781 cggcgtcacg tgcatattcc ggcactcgcg tacaaactcc gcggtatcaa atcccttgtc
      841 cccgcccacg gttacacgct cgcttcccgg gacatcctgc aacatggcca tggccgcatc
      901 gcgttcggct gtgcccgtcg cttcccacac caggctcgac ataatcaatc cgttgcggtt
      961 ctccacaagc agattcccgc tgtagctcaa tttcgcctcc ttccccgcgc ttttgcgcgc
     1021 caaaagcgcg tccggatcgg ttttcgattc gtgggttcgg ttcgagcgct cctcgccccg
     1081 aaagttcacc gtgggattcc ctggatcgtc cggcggaggt gaactcttct tatccttggg
     1141 ctgaaaactc ttcccactgg cccacgcttc cagcagcgta ccgtccacgc tgaagtgctc
     1201 gtccgaagtc catccctttt ctcgcgcctg cgctaccacc cgcgccagaa attccttggc
     1261 cacctcggcc tccaacaacc ggtcgcggtt cttgctgaat accgtcgcat cccacacccg
     1321 atcatccaaa ttcagtccca caaaccagcg gaacaaaatg ttgtagtcca tctcttccat
     1381 caacagccgc tcgctgcgca ccgaatacaa catctgcaac agctgcgcgc gcaacaactg
     1441 ctcgaagcag cggccctgat gctggggagc gacaagtcgc ggggctattg tttggagatg
     1501 atctgtgcgg attttgtggc gggagtgagc ttggagac
//
LOCUS       DATCBB010000389         4776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_142868, whole genome shotgun sequence.
ACCESSION   DATCBB010000389 DATCBB010000000
VERSION     DATCBB010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142868"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1039
                     /gene="panB"
                     /locus_tag="VII37_04380"
     CDS             149..1039
                     /gene="panB"
                     /locus_tag="VII37_04380"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY5068993.1"
                     /translation="MSFTSNTGSEKITVPDILHRKNQAAGSPTNQKKIACLTAYDYPT
                     ARLLDEAGVDVILVGDSLSMVVLGYDSTLPVTIEEMLHHTRAVRRGAHRALVVADMPY
                     GSYHTDMAESVRNAMRFIKDAGAEAVKVEGGERRLELIARFSEAEIPVMAHVGLTPQS
                     LNAMGGYHVQGKTPLGAEQLLRDARAVEAAGAFAIVLEAIPRELAAQITKEVRIPTIG
                     IGAGPDCDGQILVLSDILGLTFGQTPKFSRQYAGIGEIISGAVREYCEDVRNATFPSD
                     AESYHAPQSAKNRKTISITG"
     gene            1070..1579
                     /locus_tag="VII37_04385"
     CDS             1070..1579
                     /locus_tag="VII37_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5068994.1"
                     /translation="MSPDDIRFLYDYNAWANRRSLDAAAALTAEQFTKPLGSSFSSVR
                     DTLAHIWGAEWIWMERFEGRSPASLPDVGQFHNISALQTRWQEDEARLNNFVARVTQD
                     DLDGPHEYRTLKFGQYSNPLWLSMQHLVNHGTYHRGQITTMLRQLGAKPLSTDLIHFY
                     RERGAAANA"
     gene            1557..2471
                     /gene="panC"
                     /locus_tag="VII37_04390"
     CDS             1557..2471
                     /gene="panC"
                     /locus_tag="VII37_04390"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY5068995.1"
                     /translation="MAPPRTPDRRSLLVDIIRTVSWMKEFARKEREENRIVGLVPTMG
                     ALHQGHLSLFKQARQQCSSVVASIFVNPTQFGPSEDFAKYPRTLPSDTEKLENAGVQC
                     LFLPEAKEIYPENYSTYVNVEGLSDRLEGRLRPGHFRGVSTVVMKFLQIVQPQFAYFG
                     RKDAQQATIISRMAKDLNLDSEIVICPIEREADGLAMSSRNVYLDSSNRQAATALHRA
                     LHDVRLLLQIGTRDALSLQSAMHRALKEEPRAKVDYAEIVDAQTFEPLTHINRKAYVL
                     TAAKFGETRLLDNMLVDFGEDGQLTTEL"
     gene            complement(2515..3264)
                     /locus_tag="VII37_04395"
     CDS             complement(2515..3264)
                     /locus_tag="VII37_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5068996.1"
                     /translation="MSRELIRPALDDISLRIEAGEHVCILGPNGCGKSTLIKTITREC
                     YPVIREGSSISILGRERWNIFELRSLLGIVSPDLLGACTTEATGRDVVLSGFFSSTRI
                     FPHHHPDPAQLALAGRALERLGVAHLAARPVAHMSSGEAKRTLIARALVHDPRALLFD
                     EPSNALDIAAQLQLRAAMRELAQSGIAILLVTHHVSEIIPEIERVILLRDGRIIADGP
                     KAEVLTSEGISALFGIPLRISQQDGYFHIRE"
     gene            complement(3741..4028)
                     /locus_tag="VII37_04400"
     CDS             complement(3741..4028)
                     /locus_tag="VII37_04400"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY5068997.1"
                     /translation="MAAKKLTEAEVKERLSEIQGWTLQGGKLHRAFECKDFVTAFGNM
                     TRVALVAEAMNHHPEWFNVWNKVVIDLNTHSVGGISDLDFELAAKINEIFG"
     gene            4196..>4776
                     /locus_tag="VII37_04405"
     CDS             4196..>4776
                     /locus_tag="VII37_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5068998.1"
                     /translation="MKKKIRAIQYGVGPIGASIAKLMREKFAIEIIGAIDTDPAKMGR
                     DLGEVVGATDAPWGVKISDDASHVLGQAADVVIHSTSSSLPKVMDQLLACLEAESCVV
                     STCEELSYPYRTYPELAARLDTAAKEWGVALVGTGVNPGFVLDKLLVTLAAVSQRIEH
                     ASGRRVVDAAKRREPLQRKIGAGMTLDEFREKVA"
BASE COUNT         1131 a         1349 c         1253 g         1043 t
ORIGIN      
        1 taatcccagc gcgctgatca ttagaccagc ggtgctggta ccaactggct acccagggtt
       61 ctggcgattc acgcaaaatt ttgctattct ccgcaagtaa gggtacaggg ctgcatcctg
      121 aagttaagga gcggcaccgg aggccaacat gagcttcact tcgaataccg gcagcgagaa
      181 gatcaccgtt ccggacatcc tgcaccgtaa aaaccaggct gcgggctccc caaccaatca
      241 aaagaaaatt gcttgcctga cggcctacga ctatccaacc gctcgcttgc tggatgaagc
      301 cggcgtggac gtcatccttg tcggcgactc actgagcatg gtggtgcttg gatacgacag
      361 tacgctgccc gtgactatcg aagaaatgtt gcatcacacc cgcgcggttc gccgcggcgc
      421 gcatcgtgcg ctggtggttg cggatatgcc ctatggcagc tatcacaccg acatggctga
      481 atctgtgcgc aacgccatgc gcttcattaa agatgcaggc gccgaagccg taaaagtgga
      541 aggcggcgag cgccgcctgg aattgatcgc gcgattctcg gaagcggaaa ttcctgtgat
      601 ggctcacgta ggactgaccc cgcagtcctt gaacgccatg ggcgggtatc acgtgcaagg
      661 gaaaactcca ctcggcgcag aacaattgtt gcgcgatgct cgcgccgtgg aggctgccgg
      721 agcctttgca attgtgctgg aggccatacc gcgagaattg gcggcgcaga ttaccaaaga
      781 agtgcgcatc cccaccatag gcatcggcgc aggccctgat tgcgacgggc agatcctggt
      841 attgagcgat atcttgggct taacgtttgg gcagacgccc aaattttcgc gccagtacgc
      901 cggtattgga gaaataattt ccggcgcggt gcgcgaatac tgcgaggacg ttcgcaacgc
      961 cacatttcct tcggatgccg agtcttacca cgcaccgcaa agcgccaaaa atcgcaaaac
     1021 catttccatc accggctaat cgacccggtt gaacttgtct ggaggaaaaa tgtcgccgga
     1081 cgacattcgc tttctttacg attacaatgc ctgggctaac aggcgctcgc ttgatgccgc
     1141 tgccgccctg accgcggagc aatttacaaa accgctcggc tccagctttt cttccgtgcg
     1201 tgacaccctc gcgcatattt ggggagcgga atggatctgg atggaacgct tcgaaggtcg
     1261 ctcccctgct tcattacccg atgtaggaca attccataac atttctgcgc tccaaacgcg
     1321 ttggcaggag gatgaagcgc gtcttaataa ttttgtggcg cgcgttacgc aagacgattt
     1381 agatggcccg catgagtatc gcacgctgaa atttggtcag tacagcaatc cgctatggct
     1441 gtcgatgcaa catctcgtca atcacggcac ctatcatcgc gggcaaatca ctaccatgct
     1501 caggcaattg ggcgccaaac cgctttccac cgatttgatt catttttatc gcgagcgtgg
     1561 cgccgccgcg aacgcctgac cgccgctccc ttctcgtgga catcattcga actgtttcct
     1621 ggatgaaaga attcgcgcgc aaagaacgcg aggaaaatcg cattgtcggc ctggttccca
     1681 ccatgggcgc attgcatcaa ggccatctat cgctctttaa acaggctcgg caacaatgtt
     1741 cgtcggtggt cgccagtatt ttcgtgaacc ccacacagtt tggcccttcc gaggattttg
     1801 cgaagtatcc gcgcacgctg ccgtcagaca ccgaaaaact ggaaaatgct ggcgtccagt
     1861 gcctgttcct gccggaggca aaggaaattt atccggagaa ctattccacc tacgtcaacg
     1921 ttgagggctt aagcgatcgt ctggaaggcc gcttacggcc tggccatttc cgcggagtca
     1981 gcactgtagt gatgaaattt ttgcaaatcg ttcagccgca gtttgcatat tttggaagaa
     2041 aagacgcgca gcaggcgacc ataatttcgc gtatggcaaa agatctaaat ctcgactcgg
     2101 aaattgttat ttgccccatt gaacgcgaag ccgatggatt ggccatgtcg tcaaggaatg
     2161 tctatttgga tagcagcaat cgacaagctg ccacggcgtt gcatcgagct ttgcatgacg
     2221 tacgacttct tttgcaaatt ggcacccggg acgcactttc attgcagtcc gccatgcatc
     2281 gcgcgctcaa agaagagccc cgagccaaag tcgattatgc agaaattgtg gacgcccaga
     2341 catttgagcc tttaacccac atcaaccgca aagcgtacgt cctgaccgct gcgaaattcg
     2401 gcgaaacgcg gttgctggat aacatgctcg ttgattttgg tgaagacggc caactgacca
     2461 ctgaattgta gcgaacgcct tacaaatttt ctcgcggttc cgtcaatcga accatcattc
     2521 ccggatgtga aagtacccat cctgctgcga tattcgcaaa ggaatgccaa acagcgccga
     2581 gatgccttca gaagtaagca cctccgcctt cggaccgtcc gcgataatgc gcccatcgcg
     2641 aagcaaaatt acccgctcta tctccggaat aatctccgag acgtgatgcg taaccagcag
     2701 aatcgcaatt cctgattgcg ccagctcgcg catcgcagcg cgtaactgca attgcgcggc
     2761 aatatccagc gcgttgctgg gctcatcaaa aagtaaagcg cgcggatcat gcaccagcgc
     2821 ccgtgctatt aatgtgcgct tggcttcacc cgaagacata tgcgccaccg gacgagccgc
     2881 cagatgagcg acgcccaggc gttctaaagc gcgaccggca agcgccagct gagcaggatc
     2941 aggatgatga tgcggaaata tgcgcgtgct gctgaaaaat ccagagagca ccacatcacg
     3001 acccgtcgcc tcggtggtac aagcccccag aagatcgggc gaaacgatgc ccagcagcga
     3061 ccgcaattca aagatattcc agcgttcgcg ccccagaatc gagatggaag acccttcgcg
     3121 gataacggga taacactccc gggtaatggt tttaatcagt gtcgatttcc cgcaaccatt
     3181 agggcccaga atgcacacgt gctcgccagc ctcgatgcgc aaggaaatgt catcgagcgc
     3241 agggcgtatt agttcgcgac tcaactcaga ggttattcca ggagtgagcg cgttttggcc
     3301 cacgccatta tctgaggctc cacgaaaaac tgtcacatgt ttaaggttaa gtagcggagg
     3361 cgaggaactc atgcgcgcga attgggctcc gtgacaactg cgaactccat tctacgcggg
     3421 cggggtatca taagctatct agatgaaaac tgaagggtta agaacacctc gagaacaaaa
     3481 aagtaggagg tgaaagcaac agtttcacct cccggggtgg ggtgatgcat gacaagttct
     3541 tgaacgggtg cagtagcctg ccctgccagg cgagactaca gcctcattcg cttactgcgg
     3601 taaacagtgc gtcaggagac tcaccatcca aggtttgtcg ttattgcgtt tttagcatct
     3661 gcaatcggcg atgtcaacca cccaaggccc cccgattgcc gctttgtttg ctgccataca
     3721 gatccccaca gcatccgtta ctacccaaag atttcattaa tttttgccgc cagctcgaaa
     3781 tccaggtcgc tgatgccgcc gacactatgg gtattcagat cgattaccac tttattccac
     3841 acgttaaacc attccgggtg atggttcatg gcttccgcca ccaacgctac gcgagtcatg
     3901 tttccaaaag ccgtcacgaa gtccttgcat tcgaaagcgc gatgcagctt cccaccttgc
     3961 aacgtccatc cttgaatctc actcaaacgc tctttgactt ccgcttccgt aagttttttt
     4021 gccgccattg gagttctccg attttgcaag tatgcggcga tccaacaccc gcagcaagcc
     4081 tcctgcgcgg atttatgggc tttggcgttg cctgcactag cgaattcctc tatcgtaggg
     4141 gtttcgtttt cataagaaat gcgcggcgtg ccacagccgt ccaaaggcca acaaattgaa
     4201 aaagaaaatt cgcgccattc aatatggcgt cggtcccatc ggcgcttcca tcgctaagtt
     4261 gatgcgagaa aaattcgcca ttgaaattat tggtgccatc gataccgacc ccgccaaaat
     4321 gggccgcgac ctcggcgaag ttgtcggcgc caccgatgct ccctggggcg tgaaaatttc
     4381 agacgacgcc tctcacgtgc tgggccaagc cgcggacgtg gtgattcatt ccacttcatc
     4441 gtcgctgccg aaggtcatgg atcaactgct ggcctgcctg gaagccgaat cctgcgtggt
     4501 gtcaacgtgc gaagagcttt cctatccata tcgcacttat cccgagctgg ccgcgcgtct
     4561 cgacaccgcc gcgaaagagt ggggcgtagc gcttgtgggt acaggcgtca atcccggatt
     4621 tgttctggac aagctgttgg tcactcttgc cgcggtgtcg caacgcattg agcatgccag
     4681 tggacgccgc gtcgtcgacg ccgccaaacg ccgggagccg ctacaaagga aaattggcgc
     4741 gggcatgacc ctcgatgaat tccgtgaaaa agtagc
//
LOCUS       DATCBB010000390         1027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_369354, whole genome shotgun sequence.
ACCESSION   DATCBB010000390 DATCBB010000000
VERSION     DATCBB010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369354"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..635)
                     /gene="hpt"
                     /locus_tag="VII37_04410"
     CDS             complement(33..635)
                     /gene="hpt"
                     /locus_tag="VII37_04410"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY5068999.1"
                     /translation="MTPARKSSTAPLRSELAHESVRVLIPRERIKKCIAEMAARIRKD
                     FEGEPLHLVGVLKGAVFFLADLARQIPGEVSFDFIAVSSYGSSTHSSGQVRLTRDLDS
                     SIEGKTVIVVEDILATGKTMQYLLSIFQQRKPKHLRVAVLLDKPERRITAVQADYVGF
                     RIPNEFVVGYGLDYAERYRNLLDVGVLTIGKAKKKKGSPK"
     gene            748..>1027
                     /locus_tag="VII37_04415"
     CDS             748..>1027
                     /locus_tag="VII37_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5069000.1"
                     /translation="MTGPTGQTEPWLRGTHSDVADVPRAAMHAFEQAGEDVTLWCAEL
                     TDAELNATPYGLPSIAFQLVHIAGSLDRLLTYAEGRSLDEQQLAALKSE"
BASE COUNT          225 a          302 c          279 g          221 t
ORIGIN      
        1 cgccgtttca ccggagttgc cgggcagcgc agtcatttcg gcgagccttt ctttttcttg
       61 gccttcccga tggtcaagac tcccacatcg agcaagttgc gataacgctc agcataatcg
      121 agcccgtagc ccaccacaaa ctcgttggga attctgaatc caacataatc cgcctgcacc
      181 gcggtgattc gccgctccgg tttgtccagc aggaccgcga cgcgcaaatg ctttggcttg
      241 cgctgctgaa aaatgctcag cagatattgc atggtcttgc cggtagccag aatatcttcc
      301 accacgatta ccgtttttcc ctcgatgctg gaatccaggt cccgcgtcaa acgcacctgc
      361 cccgacgaat gtgtgctgct gccatagcta gacacggcaa taaaatcgaa agaaacttct
      421 cccggtatct gccgcgccag atcggccaga aaaaataccg cgcctttgag caccccgacc
      481 aggtgcaaag gctcgccttc aaaatctttg cggattctcg ccgccatctc cgcaatgcac
      541 ttcttgatgc gctcgcgcgg gataagcacg cgcacgcttt cgtgcgccag ctcggaacgg
      601 agcggtgcgg tgctggattt gcgcgctgga gtcatcgatt tgaaccgaat cgagactcta
      661 acgcacggct cgctcgcaga gcaagcgccg cgcatactca atgttcgcga tccatgttta
      721 aaatggagtt aggatgagag cgatcaaatg actggaccga ctggacagac tgagccgtgg
      781 ctgcgtggaa cacattcaga cgttgcggat gtaccgcgcg cggcgatgca cgcgtttgaa
      841 caagccggcg aggatgtaac tctctggtgc gctgaattga ccgatgcgga actgaacgcc
      901 acgccgtatg gcctgccgtc gattgcattt cagctagtgc acatcgcggg cagcctggat
      961 cgcttgctca cttatgctga aggacgctcg ttggatgagc agcagctcgc cgccttgaaa
     1021 agtgaag
//
LOCUS       DATCBB010000391         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_305092, whole genome shotgun sequence.
ACCESSION   DATCBB010000391 DATCBB010000000
VERSION     DATCBB010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305092"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VII37_04420"
     CDS             complement(<1..1070)
                     /locus_tag="VII37_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5069001.1"
                     /translation="MADQKLITLKINDQDVRVSPGMLVIEATRRIGTEVPSFCYYPGL
                     SLQAACRMCLVEVEKMPKLQTACTLVATEGMIVRTNTEQVRNARKYMLEFLLTNHPLD
                     CPVCDKGGECELQDMVFRYGADTSRFIEEKIHRPEEKWSELVYYDAPRCILCFRCVRV
                     CDEGMDVKALGVGMRGANSVIIPNRGDHLDCEECGMCIDICPVGALTSGTYRYKTRPW
                     EMKYAPTICTHCSNGCKTTLSVRNHEIMRSNNRDLSGINKDFLCVKGRFGFDFTKHPE
                     RVKQPMLRRGSELYPVSWEEAAQAAATKLKNVVDKGGKDAIGFIGSNRTSNEENYLFQ
                     RLARQTFGTNNVDHHRTVDYTG"
     gene            complement(1088..1519)
                     /locus_tag="VII37_04425"
     CDS             complement(1088..1519)
                     /locus_tag="VII37_04425"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5069002.1"
                     /translation="MPLLTADEIDIPMDYDSVAKAGSMLGSGGMVVMDEDTCMVDMAR
                     RIMHFYAHESCGWCIPCREGTTWLRKMLERLHAGYGREQDIDMLSELSKNILGRSFCP
                     LGDAAAMPTISIVQKWRNEFEDHLRGRCAYKSAEAALAGTR"
     gene            complement(1507..2898)
                     /gene="nuoE"
                     /locus_tag="VII37_04430"
     CDS             complement(1507..2898)
                     /gene="nuoE"
                     /locus_tag="VII37_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY5069003.1"
                     /translation="MSPGLTARFERLQNAYPVKRSALIPMLMYAQDEFGYISDDVIAE
                     IAARLELRTVQVEETLEYYSMLHRKPMGKYHVQVCTNVACMLRGGNELLDRAKKRLEI
                     GHKEVTKDGIFSLEEVECIGACTGAPAMQINYDFYENLTPATFDRLIQELDFGRRDEP
                     VTVISGALHAREAGEIPVISKRWGIENSTKIDVFLQNGGYQALDKALKQMTPESIIDE
                     MKKSNLRGRGGAGFPTGMKWSFVPKDSPKAKYVICNADESEPGTCKDRPLMEMDPHQL
                     IEGMVIAGRAIGSHQGFIYIRGEYRYVLDIVQGAITEAYERGYLGKNILGSGFDYDLL
                     IHTGAGAYECGEESALMESLEGKRGYPRIKPPFPAVVGLYGCPTIINNVETLSAVPTI
                     ILGGGEAYANLGTPKNGGTRLLCVAGHVNKPGIYEIPLGMNMKEFIYGMAGGITGGKK
                     LKAVIPGGSSCHC"
     gene            complement(2911..>3509)
                     /locus_tag="VII37_04435"
     CDS             complement(2911..>3509)
                     /locus_tag="VII37_04435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY5069004.1"
                     /translation="IWMGRTKNIGVISAEDAIALSMTGPTIRAAGVAYDNRKLFPYSS
                     YDEFEFDVPTRTDSDCFGRYIIRVAEMRESLKIVRQALDKITDQGAIKSDAPGIVPPQ
                     REKMKTEMEALIYHFKIFTEGFSPPAGEAFVTVESPRGELGCFIASDGSSKPLRVHFR
                     SPSFINLQALPKLVEGQLISDVIACIGTIDIVLGEVDR"
BASE COUNT          749 a         1142 c          805 g          813 t
ORIGIN      
        1 aagccggtat agtccaccgt gcggtgatga tcgacattgt tggtgccaaa ggtttgccgc
       61 gccagccgct ggaataaata attctcttcg ttcgacgtgc gattcgaacc gataaatccg
      121 atcgcgtctt tcccgccctt atcaacaacg ttcttgagct tcgtcgccgc cgcctgcgcc
      181 gcctcttccc atgaaacggg ataaagttcg ctgccgcgtc gcagcatcgg ctgcttcacc
      241 ctctccggat gtttcgtaaa atcaaaaccg aatcgtccct tcacgcacaa aaagtctttg
      301 ttaatcccgg acaaatcccg gttgttcgaa cgcatgattt cgtgatttct cacgctcagc
      361 gtggtcttgc agccattcga acaatgcgtg caaatcgtcg gcgcgtattt catttcccac
      421 ggccgcgtct tgtagcgata agttccgctg gtcagcgccc ccaccggaca aatatcgatg
      481 cacatcccgc attcttcgca gtccaggtga tccccgcggt tcggaatgat cacgctgttt
      541 gcgccgcgca tccccacgcc cagcgctttt acatccatgc cttcgtcgca cacgcgcacg
      601 cacctgaagc acaaaatgca tcgcggcgca tcgtaataca ccagctccga ccacttttct
      661 tccggccggt gtattttttc ttcgatgaac ctgctggtat ccgcgccgta gcggaaaacc
      721 atgtcctgca gttcgcactc gccgccttta tcgcataccg ggcaatccag cgggtgattg
      781 gtcagcaaaa attccagcat atacttccgc gcgttgcgca cctgctcggt attggtgcgc
      841 acgatcatgc cttcggtcgc caccaaagtg cacgccgtct gcaacttcgg catcttctcc
      901 acttccacca ggcacatgcg gcaagccgcc tgcaatgaca accccgggta gtagcagaac
      961 gacggcacct ccgtgccgat gcgccgcgta gcttcaatca ccagcatccc aggcgacact
     1021 cgcacgtcct ggtcattaat cttcagcgtg ataagttttt ggtcagccat tgagtaaggt
     1081 cattcgttta tcgcgtacca gccagcgccg cctcagcgga tttatatgcg cagcggccac
     1141 gcagatgatc ttcaaattca ttccgccact tttgcacgat actgatcgtc ggcatcgccg
     1201 cggcatcacc aagcgggcag aaactccgtc ccagaatatt tttagagagc tcgctgagca
     1261 tgtcgatatc ctgctcgcgc ccatatcccg cgtgcagccg ctccagcatc tttcgcagcc
     1321 acgtcgttcc ttcgcggcag ggaatacacc atccgcacga ttcatgcgcg taaaaatgca
     1381 taatccgccg ggccatgtcc accatgcagg tatcttcgtc catcaccacc atgccgccgg
     1441 aacccagcat ggaacccgct ttggccaccg aatcgtaatc catcggaata tcgatctcgt
     1501 cggcagtcag cagtggcacg aactgccgcc cggaatcacc gctttcaatt tcttgccgcc
     1561 tgtgatcccg ccggccatgc cataaataaa ttccttcatg ttcatgccca ggggaatttc
     1621 gtaaatccca ggcttgttca catgtccggc cacgcacagc aatcgcgtgc cgccattttt
     1681 tggcgtccct aaatttgcat acgcttcgcc gccgcccaga ataatcgtag gcaccgcgct
     1741 caacgtctcg acattattaa taatcgtcgg gcagccatag agcccaacca cagcggggaa
     1801 gggaggctta attcgcggat agcctcgttt gccttccagc gattccatca acgccgattc
     1861 ttcgccgcat tcatacgcgc ccgcgccagt gtgaatcagc aaatcatagt caaatcccga
     1921 accgagaata ttcttgccca ggtatccgcg ctcgtatgct tccgtaatcg cgccttgcac
     1981 aatgtccagc acatagcgat attcgccgcg aatgtaaata aatccctggt gcgacccaat
     2041 cgcgcgcccc gcaatcacca tgccctcgat cagttggtgc ggatccattt ccatcaaagg
     2101 gcgatccttg caagtgcccg gctcggactc atccgcgttg caaatcacgt atttcgcttt
     2161 cggcgaatcc ttgggtacga acgaccactt catgccggtc ggaaatcccg cgccgccccg
     2221 tccgcgcaag ttcgacttct tcatctcatc gatgatcgac tccggcgtca tctgtttcag
     2281 cgctttatcc agcgcctggt agccaccgtt ctgcagaaac acgtcaatct tggtcgagtt
     2341 ttcgatgccc cagcgcttgc tgataaccgg aatctcgccc gcttctcgcg cgtggagcgc
     2401 cccggaaata accgtaaccg gctcatcccg ccgcccgaaa tctaattcct gaatcaggcg
     2461 atcgaaagta gccggcgtca ggttctcgta aaagtcgtaa ttaatctgca tcgctggcgc
     2521 cccggtacaa gccccgatgc attccacttc ttccagcgaa aaaatcccgt ccttggtaac
     2581 ttccttgtgt ccgatttcca gccgcttctt cgcgcgatcc agcaattcgt taccgccgcg
     2641 cagcatgcac gccacattgg tgcacacctg cacgtgatat ttccccattg gcttgcgatg
     2701 cagcatcgag taatactcga gcgtctcctc aacctgcacc gtgcgcagct ccagccgcgc
     2761 cgcaatttcc gcgatcacat catcgctgat atacccaaat tcatcctgcg catacatcag
     2821 catgggaatc agcgcgctgc gcttcaccgg atatgcgttc tgcagccgtt cgaatcgcgc
     2881 cgtaaggccc ggggacattt gcagtgccat ctagcgatcc acctcgccca gcacgatatc
     2941 gatcgtgccg atgcacgcaa tcacgtccga aatcaattgc ccttcgacca atttcggtaa
     3001 agcctgcaaa ttaatgaatg atggcgaacg gaaatgcact cgcagcggct tcgatgatcc
     3061 gtcgctcgca ataaagcaac ccagctcccc gcgcggcgat tccacggtca caaacgcttc
     3121 gccagccggt ggagaaaatc cttccgtgaa aattttgaag tgataaatca gcgcttccat
     3181 ctctgtcttc atcttctcgc gctgtggcgg cacgatcccc ggtgcatcac ttttgatcgc
     3241 gccctggtct gtaattttgt ccagcgcctg ccgcacgatc ttcaggcttt cgcgcatctc
     3301 cgcgacacgg attatgtacc gcccaaaaca atccgaatcc gtgcgtgtgg gcacatcaaa
     3361 ctcaaattcg tcataactcg agtacggaaa caatttgcgg ttatcgtaag ccacgcccgc
     3421 tgcgcgaatc gtgggcccgg tcatgctcaa cgcaatggca tcttccgcgc tgataactcc
     3481 aatgtttttt gtgcgcccca tccagatgc
//
LOCUS       DATCBB010000392         1936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_45876, whole genome shotgun sequence.
ACCESSION   DATCBB010000392 DATCBB010000000
VERSION     DATCBB010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45876"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VII37_04440"
     CDS             <1..519
                     /locus_tag="VII37_04440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5069005.1"
                     /translation="RMGVSDERTGLLSRSSYQSCLLTEADRARTQGTPLALAILQIDR
                     GSDILRQQGESPMERFLEQLARSLQSMLRQNDVAVKYTSWSLAFILPDTTLEGAQHLA
                     DKLLRAASGLRPPWNATQITLSAGIVEATAKPEYDSEDIVTDLINRAEFSVEEARKRG
                     GATVIALESPVV"
     gene            550..1740
                     /locus_tag="VII37_04445"
     CDS             550..1740
                     /locus_tag="VII37_04445"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5069006.1"
                     /translation="MFPTDVVIVAGARTPMSRYTGSFSEVSAIDLAAHASKEAIRRSG
                     VDPGDFDHAIFGNVMQTSADALYGARHVGLKAGLKIEAPAVTVNRLCGSGMESIAQAA
                     QRLLLGEATMVLAGGMENMTQAPFVIRGARNGLKLGGGALEDTLMAGLTDSYCGLPMA
                     LTAEKLAEQHGITRADADTYALRSQQAADAAYKACRVRDELVPVEVKHGKKSITVSED
                     DHRRPETTLETLAKLPPSFKKDGIVTAGNASGIVDGAAAVVVTREQTAKDRGLKPVGR
                     IVAWAVAGVDPSIMGIGPVPSSQKALKLAGLSLEQMDRVEVNEAFAAQYLAVEKALGL
                     NRDKTNVNGGAIALGHPLGATGTRLVITILNELRRNNLRYGLTTACIGGGQGIAMIVE
                     ALYN"
BASE COUNT          408 a          413 c          711 g          404 t
ORIGIN      
        1 cgcatgggcg tttccgatga acggacgggc ttattgagcc gcagctcgta tcagagttgt
       61 ttgttgacgg aagcggatcg cgcgcggacg caggggacac ctttggcgct ggctattctg
      121 caaatcgatc gtggctcgga cattttgcgg cagcagggcg aatcgccgat ggagcgattc
      181 ctggagcaac tggcgcgttc gctgcaatcc atgttgcggc agaatgatgt ggcggtgaaa
      241 tacacttcgt ggtcgctggc gtttattttg ccggatacga cgctggaggg ggcgcagcat
      301 ctggcggaca aattattgcg ggcggcgtct ggtttgaggc cgccgtggaa cgcgacgcag
      361 atcactttga gcgcaggaat cgtggaagct acggcgaaac cggagtacga cagcgaagat
      421 attgttacgg atttgatcaa tcgcgcggaa ttttccgtgg aagaagcgcg taaacgcggc
      481 ggggcgacgg tgatcgcgct ggagagtccg gtagtctaga gaataattca gaaatcagag
      541 aggaatttta tgtttcccac agatgttgtg attgttgctg gcgcgcgcac gccgatgtcg
      601 cgttatacgg gatctttttc ggaggtgtcg gctatcgact tggcggcgca cgcatcgaag
      661 gaagccattc ggaggtcggg agttgatccg ggggattttg atcacgcgat ttttggaaat
      721 gtgatgcaga ccagcgcgga cgctttatat ggcgcgcggc acgtggggtt aaaggcggga
      781 ttgaaaatcg aggcgccggc agtgacggtg aatcgtttgt gcggatcggg catggagtcg
      841 attgcgcagg cggcgcagcg attattgctg ggcgaagcga ccatggtgct ggccggcggc
      901 atggaaaata tgacgcaggc gccttttgtg attcgcggcg cgcgcaatgg actgaaactt
      961 ggcgggggcg cgctggaaga tacgctgatg gcggggctga cggattcgta ttgcggattg
     1021 ccgatggcac tgacggcgga aaaacttgcg gagcaacacg gcattacacg cgcggatgcg
     1081 gacacttacg cgttgcgcag ccaacaagcc gcggatgcag cgtacaaggc gtgccgcgtg
     1141 cgcgatgagt tggtgccggt ggaagtgaag cacgggaaga aatctattac ggttagtgaa
     1201 gacgatcatc gccgacctga gactacgctg gagacgctgg cgaagttgcc gccttcgttc
     1261 aagaaagatg ggattgttac cgcggggaac gcgagcggga ttgttgacgg tgcggcagcc
     1321 gtggtggtca cgcgggaaca aactgcaaaa gaccgcgggc tgaagcctgt ggggcggatc
     1381 gtggcgtggg cggtggcggg cgtggatccc agcatcatgg ggatcgggcc ggtgccatct
     1441 tcgcaaaagg cgctaaagct tgcgggattg agtttggagc agatggatcg cgtggaagtg
     1501 aacgaggcgt tcgcggcgca atatttggcg gtggaaaaag ctttgggatt aaatcgcgac
     1561 aaaactaacg tgaacggcgg ggcgattgcg cttgggcatc cgctgggagc gacgggaacg
     1621 cggctggtga ttaccattct gaatgaattg cggcgtaata atctgcgtta tggactgact
     1681 accgcgtgca ttggcggcgg gcagggcatc gcgatgattg ttgaggcgtt gtataactaa
     1741 cggtacttga tgggaacgcg gcggcacgtt ctgcgaattc cacgaagaag aatttcttaa
     1801 ggggaacgga tgacgattca aaaagttggg gttgtggggt gcgggttgat gggcgctgga
     1861 atcgcgcagg tggctgctgc ggcgggtttc cagacggtgg tgcgcgaagt gagcggggaa
     1921 gttgttgaga aggggt
//
LOCUS       DATCBB010000393         1393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_175417, whole genome shotgun sequence.
ACCESSION   DATCBB010000393 DATCBB010000000
VERSION     DATCBB010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175417"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VII37_04450"
     CDS             complement(<1..1212)
                     /locus_tag="VII37_04450"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY5069007.1"
                     /translation="MPEQFSFSGLRELFAKANEEKSGDRLAGIAAGSERERIAAKRKL
                     ADLSLDEIVRQPLIGPDEDDVSALILESFDQGAFTLIKSTTVGEFREFLLDDATGEAD
                     IKRIQRAIIPEIAAAVTKIMSNKDLVLAAAKIRNVTRCRNTMGERGVLGIRTQPNHPS
                     DDIAGILLSAFEGLLYGCGDAVIGVNPASDAVESTDAILRALDRLVTTYKIPTQTCCL
                     AHISTQLAALSKRAPVDLLFQSVGGTEATNRSFGITLEMLTEGQQRVIEHHRQRQDVP
                     WIGEEVMYFETGQGSSLSAEAHCGLDQVTLEARAYGVARAFNPFLVNSVVGFIGPEYL
                     FDERQIIRAGLEDHFMGKLLGLPMGCDVCYTNHAAADQNSADNLLLLLAAAGCNYFMG
                     VPCADDGMLNYQ"
BASE COUNT          319 a          378 c          373 g          323 t
ORIGIN      
        1 ttgataattc agcatcccgt cgtccgcgca gggtacgccc ataaaataat tgcagccggc
       61 tgcggccaga agcaaaagaa gattgtccgc ggagttttga tccgccgcgg catgattggt
      121 gtagcaaaca tcgcagccca tgggaagccc cagcagcttg cccatgaagt gatcttcgag
      181 gcccgcacga ataatctggc gctcatcgaa aagatattca ggaccaatga atccaacaac
      241 actgtttacc agaaatggat taaatgcgcg cgctacgcca taggcgcgag cttccaaagt
      301 gacctgatcc aggccgcagt gcgcctccgc ggataaggag cttccctggc cggtctcaaa
      361 atacatcacc tcttcgccga tccaaggcac gtcttgacgc tgacgatggt gttcgataac
      421 gcgctgttga ccttctgtca acatttcgag cgtaataccg aaacttcggt tggtggcttc
      481 tgtaccgccc acggattgga ataagagatc tactggcgcg cgcttgctga gcgcggccag
      541 ttgcgtagaa atatgtgcga ggcagcaggt ttgcgtgggt attttgtagg tggtcacaag
      601 acgatcgagg gcgcgaagaa ttgcgtcagt gctctcgacg gcgtccgatg cgggattcac
      661 accaatcacc gcgtctccgc aaccgtagag caaaccttca aaagcggaca gcagaatgcc
      721 agcgatatcg tcgctagggt gattcggctg cgtgcgtatt cccaagactc cgcgctcacc
      781 catagtattt cggcaacgcg taacgtttcg gatttttgcg gcggctagaa ccagatcctt
      841 attactcatg atttttgtta ccgcggcggc gatttcgggg atgatcgccc gttgaattct
      901 ctttatgtcc gcttcgcctg tggcatcgtc gagaagaaac tcgcggaact cgcctaccgt
      961 ggtgctcttg atcagcgtaa aggctccctg atcaaaggat tccagaatca acgcgcttac
     1021 gtcgtcttcg tcaggaccta tcaagggctg gcggacgatt tcatcgagcg acaaatccgc
     1081 gagcttacgc ttggcggcga tccgttcccg ttctgatcct gcagcgattc ccgccaagcg
     1141 atcgccagac ttttcctcgt ttgctttggc aaacagctct cggaggccac taaaggaaaa
     1201 ttgctcaggc ataaagcgca ttgtagccta gtgcccattc gcggatgtgt taacgtcttg
     1261 ccgcggtctt caagcaggat ccaaaggcaa agggaattca tggccaaata tttgccacga
     1321 aaggtagctc tagcaagaag tgttgccgtt tgcgcgtgcg tcgcgctagc actgtcagct
     1381 ttttgcaatc gca
//
LOCUS       DATCBB010000394         1267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_467334, whole genome shotgun sequence.
ACCESSION   DATCBB010000394 DATCBB010000000
VERSION     DATCBB010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467334"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1267)
                     /locus_tag="VII37_04455"
     CDS             complement(<1..>1267)
                     /locus_tag="VII37_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069008.1"
                     /translation="REISTGNFDYQVPDQVQDELGVLARSFNKMTRQLRDSRSQIDQF
                     TRNLQQAVQELERRRQLMETVLENIPTGVVSLDASGAILRCNTAVNGMLGADSRDKQS
                     LDDLLGTEASRSVQYLMRRSLRMGVVSREIETVAGGRVLHLAVTVSPLGPRRGNTGYV
                     LVLDDLTEMLRSQKSAAWQEVARRIAHEIKNPLTPIQLSAQRLLRFIDRRPASDSKPV
                     DTELTKLVQECSRLIEREVATLAGLVNEFSQFVRFPTAKLVPTEANVIVQEALEVFSN
                     RLDGVTIKTMLAENLPQIRADAGLLRSVLINLIDNAAESLEHSACREIVVSTRNRAEA
                     ETVEISVADTGHGISPEDKDKLFLPQFSTKDRGTGLGLAIAARIIAEHAGTIHAEDNH
                     PAGSRFVIELPVTEAVAVAVGEPQAPAPET"
BASE COUNT          268 a          366 c          362 g          271 t
ORIGIN      
        1 cagtttccgg cgccggtgct tgtggctcgc caacagccac ggcgacagct tccgtcacgg
       61 gcagctcgat cacaaagcgc gagccggcag ggtgattgtc ttccgcgtga atcgtgccgg
      121 cgtgttcggc aataatgcgg gcggcgatag ccagcccgag accggtgccg cggtccttgg
      181 tagaaaattg cggcaggaag agtttgtctt tatcttcggg cgaaataccg tgaccggtgt
      241 cggccacaga aatttcgacg gtttcagcct ccgcgcgatt gcgagtagaa acaacgatct
      301 cgcggcacgc ggaatgctcc agtgactccg cggcattgtc gatcagattg atcaacacac
      361 tgcgcaataa gcctgcgtcc gcgcgtattt gcggaaggtt ttcggccagc atcgttttga
      421 tggtcacgcc atccaggcga tttgaaaaca cttctaatgc ttcctgcacg atgacgttgg
      481 cttccgtggg caccagcttg gcggtaggga aacgcacaaa ttgagaaaac tcgttgacca
      541 ggcccgccag cgtggccacc tcgcgttcaa tcaaccgcga gcattcctgc acgagcttgg
      601 ttagttctgt atcgacgggc ttcgagtctg aagcgggccg acgatcaata aagcgcagca
      661 agcgttgcgc ggaaagctga atgggcgtca gcggattttt tatttcgtgc gcgatgcgtc
      721 gcgccacttc ctgccacgcg gcggattttt gcgagcgcag catttcggtg aggtcatcta
      781 gcacgaggac gtagccagtg ttgccacggc gcgggcccag agggctcact gtgacggcca
      841 gatgtaaaac gcggccgcca gccacggttt caatctcgcg cgacacgact cccatacgca
      901 aagagcgtcg catcaggtat tgcaccgaac gtgaagcttc tgtcccgagc aaatcgtcca
      961 gtgattgctt gtcacgcgaa tcagcgccca acattccatt gaccgcggta ttgcagcgca
     1021 gaatggcgcc gctggcatcc agagatacaa ccccagtggg aatattttcc agtaccgtct
     1081 ccatcagctg gcgccggcgt tccagttctt gcaccgcttg ctgcagattg cgcgtgaact
     1141 gatcaatttg cgaacggcta tcgcgcaact gcctggtcat cttattgaac gaacgagcca
     1201 agacgcccaa ctcgtcctgc acttgatccg gcacttgata atcgaaattc cccgtagaga
     1261 tttcgcg
//
LOCUS       DATCBB010000395         1325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_239363, whole genome shotgun sequence.
ACCESSION   DATCBB010000395 DATCBB010000000
VERSION     DATCBB010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239363"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VII37_04460"
     CDS             complement(<1..642)
                     /locus_tag="VII37_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069009.1"
                     /translation="MHAYWCATAFLWARSSEMESTSFERANFRALEEATCADWRIMVK
                     AEADDRLKRTTGDRLLHLLASMERDDCLGSPVNLYVHSLQTATRVLRAREDDELVVVA
                     LFHDLPEALSDNHHGLMAAELLAPWISERRTWLLVHHVDFQRFHFVNHPAPDAHIRDR
                     YAGHPFYSETAYFCKFYDQNSFDRDFATLPLREFVPIIRRFFARPRPPLKPLRT"
     gene            666..821
                     /locus_tag="VII37_04465"
     CDS             666..821
                     /locus_tag="VII37_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069010.1"
                     /translation="MFVPTQDRDEPGADFTHKQGDVVRISSPRLGTLMNRVGYNQIAL
                     WTFGATN"
     gene            867..>1325
                     /locus_tag="VII37_04470"
     CDS             867..>1325
                     /locus_tag="VII37_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069011.1"
                     /translation="MNATSIYDKFRVSTVINAVGPVTRLGGSAVDSDVAGVMAEAAQY
                     CVDIGELQGRASELISELTSAEAGIVTSGASAGLLLASAACLAGMNFLKMSQLPDTAG
                     MRNEIIVPRIQRNSYDHAVRAAGARLIDVGCSDTAGNIGSCDTEAWELRSA"
BASE COUNT          300 a          361 c          396 g          268 t
ORIGIN      
        1 ggtccgcaat ggcttaagtg gcggacgcgg gcgagcgaag aagcggcgta tgatcggcac
       61 aaattcacgc agcggcaagg tcgcaaagtc ccggtcaaag gagttctgat cgtaaaattt
      121 acagaaatac gccgtctccg aatagaacgg gtggccggcg taacgatcgc gaatgtgggc
      181 gtctggggcc ggatgattaa caaaatggaa ccgctggaaa tcaacgtggt gcaccaatag
      241 ccaagtgcgt cgctcggata tccacggcgc aagcagctcg gccgccatca aaccatggtg
      301 attgtcgctc agcgcttcag gcagatcatg aaagagagca acgacgacga gttcgtcatc
      361 ttcgcgggcg cgcaggacgc gcgtcgccgt ctgcagtgag tgcacgtaca ggttaacggg
      421 actgccgagg caatcgtcgc gttccatcga tgcgagcaga tgtaaaagcc gatcgcccgt
      481 cgtgcgcttg aggcgatcat ccgcctcagc cttgaccata atccgccagt cggcgcaagt
      541 ggcctcctca agcgcacgaa agtttgcgcg ctcaaagctc gttgattcca tttcactgct
      601 cctagcccat aaaaatgccg ttgcacacca gtacgcgtgc atgcatattg tagacttatt
      661 acacgatgtt tgtccccact caggaccgcg atgaaccggg cgccgatttc acgcacaaac
      721 agggcgacgt cgtgcgcatc tcttcgccgc ggctcgggac tttgatgaac cgcgtgggct
      781 acaatcaaat tgcactatgg acattcggcg caactaactg atgcgcaaat tggctgcgcg
      841 cggctatttt taattggact gcaaccgtga atgcaacatc catctacgac aaattccgag
      901 tgtcaacggt tatcaatgcg gtcggacctg tgacccgcct tggtggcagt gcggtggatt
      961 ccgacgtcgc tggggtgatg gcagaagctg cgcaatactg cgttgatatc ggtgaattgc
     1021 aggggcgcgc tagtgaactc atttccgaac ttaccagcgc ggaggccgga atcgtgacca
     1081 gtggcgcaag cgccggcctt ctgttggcaa gcgcagcatg cctggcgggc atgaatttcc
     1141 tcaagatgag ccaactgccg gataccgctg gtatgcggaa tgaaattatc gtgccgcgta
     1201 tccagcgcaa ttcgtacgat cacgcggtgc gcgctgcggg tgcccgcttg attgatgtgg
     1261 gatgctcgga taccgcggga aacattggat cctgcgacac cgaggcgtgg gagctacgta
     1321 gcgcc
//
LOCUS       DATCBB010000396         1000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_13154, whole genome shotgun sequence.
ACCESSION   DATCBB010000396 DATCBB010000000
VERSION     DATCBB010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13154"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VII37_04475"
     CDS             complement(<1..534)
                     /locus_tag="VII37_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069012.1"
                     /translation="MAILGRREVPTDGVHDYCCYLRDALANEGVNLTLTQVRWAEIGR
                     KASRQELLEAIKGKQNTLFLLQYTALSWSRRGFALPAVGILKLLKRTGARCAVVFHDP
                     DGFAGNRAIDSFRRGIQRYAMKRLLQLSDLAIFTLPRHKISWLPAETQNFVFIPVGAN
                     LPAPERAWKAYRASHGQR"
     gene            643..>1000
                     /locus_tag="VII37_04480"
     CDS             643..>1000
                     /locus_tag="VII37_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069013.1"
                     /translation="MNLLIYSHYFAPSVGGVGTIVQSLAAGIAELRTLNGDREFDVTV
                     VTETAADGYDDTKFPFRVVRRPGVIPLWQLVRASDVIHTAGPAFLPMFLAWLSRKRFV
                     VEHHGYQATCPNGLFVH"
BASE COUNT          216 a          298 c          241 g          245 t
ORIGIN      
        1 cctctgtcca tgcgaagcac gatacgcttt ccacgctctt tcgggcgctg gcaaattagc
       61 acccaccgga atgaaaacaa aattttgcgt ctccgctggg agccaggaaa tcttatggcg
      121 aggcagcgta aaaatcgcca gatcgctgag ctgcagcagc cgtttcatcg cgtagcgctg
      181 aatacctctt ctaaagctgt ctattgcgcg gtttccggcg aagccgtctg gatcgtgaaa
      241 caccaccgca caccgtgcgc cagttctctt tagtagtttc agaatgccca cagcaggcag
      301 cgcaaatcct cgtcgtgacc acgacagcgc cgtgtattgc aaaagaaaaa gtgtattttg
      361 tttgcccttg atggcttcca gcaattcctg ccggctggcc ttgcgtccaa tttccgccca
      421 tctcacctgc gtcagcgtta aattcactcc ctcattcgcc agcgcatccc ggagatagca
      481 gcaataatca tgcactccgt ctgtaggcac ttcgcggcgt cccagaattg ccatgcattc
      541 catgagatat aatttgaccc ggaccagcgt aacatctatc ccggcaacat cacgtaacgt
      601 gagcaggccg gatattttcc atcagatgcg gccgcatcga gcatgaacct attaatttat
      661 tctcattatt tcgcgcccag tgtcggcggc gtcggaacta tcgtgcagtc actggccgcc
      721 ggcattgccg aactgcgtac actaaatggc gatcgtgaat ttgacgttac cgtggtcacc
      781 gaaacggccg cggatggcta tgacgacact aagtttcctt ttcgtgtggt ccgccgtccc
      841 ggggttatcc cgctgtggca acttgtgcgt gcctccgatg tgatccacac agctgggccg
      901 gcattcctac ccatgtttct ggcttggctc tcgcgcaagc gattcgttgt cgaacaccat
      961 ggctatcaag caacttgccc taatggacta tttgtccacc
//
LOCUS       DATCBB010000397         1646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_207655, whole genome shotgun sequence.
ACCESSION   DATCBB010000397 DATCBB010000000
VERSION     DATCBB010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207655"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VII37_04485"
     CDS             <1..926
                     /locus_tag="VII37_04485"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5069014.1"
                     /translation="EISKAIVGQGGVIEGVLIALLAGGHVLLEGAPGLGKTMLVRTLA
                     QAVALKYSRIQFTPDLMPADVIGTSVVQENGDGRKSLGFQPGPIFTNLLLADEINRAT
                     PKTQSALLEAMQEKQVTVAHTSHLLEEPFFVMATQNPLEMEGTYPLPEAQLDRFLFKL
                     RVPYPGREDLHDILDRTTQGTIAVVCPVVNGAQILEMRKVVRTVPVAPHVQEYVVKLT
                     LATHPENRENLLASKYIRYGSSPRGAQTLILGGKVRALLDGRYNVSMEDIRALMLPAL
                     RHRIILNFEAQADGRTPDDLLEEIAGKVRAK"
     gene            1316..>1646
                     /locus_tag="VII37_04490"
     CDS             1316..>1646
                     /locus_tag="VII37_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5069015.1"
                     /translation="MEFADYRRYSFGDDPRSLDWSAYLRLGKLFLKIYETEQHIPVRI
                     LLDRSESMNFGDEGESKFLYAQRLAATFAYLALLHLDTTAVIPFSDRLQKPLIVSGGR
                     ERLWPVLQ"
BASE COUNT          338 a          489 c          483 g          336 t
ORIGIN      
        1 gggaaatttc caaggcgatc gtcggccagg ggggagtgat cgagggcgtg ctgatcgcct
       61 tgctggccgg cgggcacgtg ctgcttgagg gcgcgcccgg cctcgggaag acgatgctgg
      121 tgcgcacgct tgcgcaggcc gtggcgctga aatattcgcg cattcagttc actcccgatt
      181 tgatgccggc cgacgtgatc ggcacctccg tggtgcagga aaacggggac ggaaggaaat
      241 cgctgggatt ccagcccggc cctatattta ctaatttgtt actggcggat gaaattaatc
      301 gtgcgacgcc caaaacgcaa tccgctctgc tcgaggccat gcaggaaaag caggtcaccg
      361 tggcccacac ctcgcacctg ctcgaggaac cctttttcgt gatggccacg cagaatccgc
      421 tcgaaatgga agggacctac ccgctacccg aagcgcagct cgaccggttc ctcttcaaac
      481 tgcgcgtccc ctatccaggg cgggaagacc tgcatgacat tctggaccgc acgacgcagg
      541 ggacgatcgc cgtcgtgtgc cccgtggtca acggcgcgca aattctggag atgcgaaaag
      601 tcgtgcgcac ggttcctgtt gcgccgcacg tgcaggagta cgtcgtgaaa ctcacgctgg
      661 ccacgcaccc ggagaatcgc gaaaatcttt tagcgagcaa atatatccgg tatggaagct
      721 cgccgcgcgg cgcgcagact ttgatcctcg gcgggaaagt gcgcgcgctc ttggacggcc
      781 gctacaacgt cagcatggaa gacattcgcg cattgatgct gcctgccctg cgccaccgca
      841 ttatcctgaa ctttgaagcc caggcggatg gccggacccc cgacgacttg ctggaagaaa
      901 tcgccggcaa ggtccgggcg aaatgaggtt tggtagcaca ggttccacag attgcggaaa
      961 aactcaaaat tttgtcattc cgaggcgcgc tccgcgccga ggaatctctc ttttcctgag
     1021 tttcaatcaa agagagattc ttcgcctcgc tcggaatgac aaaataaatt actttttccg
     1081 cagcctattt agcctgtggg ttttaggagt ggcaaggagc gaccgcgctc cgaccaaccc
     1141 gacaggctga atcctgtcct actggtgcta cgggctgaat ccgatgtcca caacttctcc
     1201 gcttttgctc gaccgagaat tcctgaaaac gctggagcag gtcacactgc tgtgccgcac
     1261 gaacctgtcc ggcgcggtgg gccccgagca ccgttcgcgc acgcgcgggc cgggaatgga
     1321 attcgccgat taccggcgct acagcttcgg cgacgatcct cgttcgctcg attggagcgc
     1381 ctatctccgc ctgggaaaac tcttcctgaa aatttacgaa accgagcagc atatccccgt
     1441 gcgcattttg ctggaccgca gcgagtcgat gaactttggc gatgagggag agtcaaaatt
     1501 tctctacgct cagcggctcg cggcaacctt cgcctatctg gcgctgctgc atttggacac
     1561 caccgcggtg attccgttct cggatcggct gcaaaaacct ctgatcgttt ccggcggtcg
     1621 cgagcggctg tggccggtgc tccaat
//
LOCUS       DATCBB010000398         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_143174, whole genome shotgun sequence.
ACCESSION   DATCBB010000398 DATCBB010000000
VERSION     DATCBB010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143174"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VII37_04495"
     CDS             <1..1698
                     /locus_tag="VII37_04495"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5069016.1"
                     /translation="EGLDVLDTPEVRDVVACLDAAVSLNDAASLFRVAALPRFGINPV
                     ELRAGMRAVRRQELDLRAVLAKVANGPAVLECVDKAHTAVDKEVEKDGVTAAQAVKIV
                     IRHFGLQRSALVATFEKFVDEWQKKPVAEGGSPAEFLEYLDYFVQAGGMVPLPRVEGE
                     AVRLLTAHAAKGLEFKHVAIIRGSSTNFPTAYREPLIAFPPELRRSEGASIDEKMLHL
                     EEERRLFYVAMTRAKDTLAIYAHQGRGKKDPKPTQFLREFMVLPNCRQYWSTRSAAAV
                     QDSLFAEEEQKIALQKSNVATWLLLPPTNAATRLSATGITIYKECPLKFKLEREWNLP
                     RDVSAALQYGAAMHRILLTFYNAQRFGREISDADLLELFRADLASAGIADRYQYELYL
                     HQGREQLTQFLQVARNAPPPEVMETERSFELAVGAATLVGRIDRIDLIDPANPDAVAI
                     VDYKTGKPQSQEDADKSLQLTLYALAARDLLGKRADRLIFHNLENNTPICSTRTDAEI
                     DSARLQVEEVAQAIAQEQFAPNPGRHCNYCSYRNLCPATEKIVPEKLTQQKKSSRLN"
     gene            2215..>2662
                     /locus_tag="VII37_04500"
     CDS             2215..>2662
                     /locus_tag="VII37_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY5069017.1"
                     /translation="MAGSNDVVIISACRTPIGKFQGSLSDLTAPKIGAVAVREAVKRA
                     NLKPEQIDECIMGNVVSAGIGQNPARQAALFGGLAPEVCAMTINKVCGSGLKAVALAA
                     QAIETGNSSIVVAGGMESMTNAPYLLPNARKGYRLGNGQLVDSMVHD"
BASE COUNT          580 a          741 c          759 g          582 t
ORIGIN      
        1 gaagggcttg acgttctcga tactcctgag gtccgcgatg tggttgcctg ccttgacgcc
       61 gcggtttctc tgaacgatgc cgccagtttg ttccgtgtgg cggcgctccc gcgcttcggc
      121 atcaatcccg tggagttgcg cgccgggatg cgggcagtgc ggcggcagga actggatctg
      181 cgcgcagtac ttgcgaaggt ggcgaacggg cccgcggttc tggaatgcgt agacaaggcg
      241 cataccgcag ttgacaaaga agttgagaaa gacggagtca ctgcggcgca ggcagtgaag
      301 atcgtgattc gccacttcgg cttgcaacga tccgcattgg tcgcgacgtt cgaaaaattt
      361 gtggatgagt ggcagaaaaa gcctgtcgcg gaaggcggca gtcccgccga atttcttgaa
      421 tacctcgatt atttcgtgca ggccggaggc atggttccac ttccgcgggt ggagggggaa
      481 gcggtgcgac tgctcaccgc gcacgcggcg aagggattgg agtttaagca tgtcgcgatt
      541 atccggggct cctcgactaa ctttccgacg gcgtatcgcg agccgctcat cgcctttccg
      601 cccgaactcc gccgctcgga gggcgcttcg atcgacgaaa agatgctgca cctggaagaa
      661 gagcggcgcc ttttttatgt ggccatgacc cgcgccaaag acacgctggc gatctacgcg
      721 catcagggcc gaggaaagaa agacccgaag ccgacgcagt ttctgcgcga gttcatggtt
      781 cttcccaatt gcaggcagta ttggtcgact cgttccgcgg cggcggtgca agacagtttg
      841 tttgccgaag aggaacagaa aattgccttg cagaaatcga acgtcgcaac ctggctgctc
      901 ttgccgccta cgaacgccgc cacgagattg agcgccaccg gcataaccat ctataaagag
      961 tgtccgctga aattcaagct tgaacgggag tggaatctgc cgcgcgatgt ttcggctgcc
     1021 ctgcagtacg gcgccgccat gcaccgcatt cttcttactt tttataatgc gcagcgtttt
     1081 gggcgcgaga tcagcgacgc ggatttgctg gaattgttcc gcgccgacct ggcttcggcg
     1141 ggcatcgccg accgttatca atatgagctc tatcttcacc aggggaggga gcagttgacg
     1201 cagtttttgc aggttgcgcg caatgctccg ccgcccgagg tgatggagac ggaacgcagt
     1261 tttgaattgg cagtgggcgc ggcgaccctg gtggggcgaa tcgatcgcat cgatcttatc
     1321 gacccggcga atcccgatgc ggtggcgatt gtcgactaca agaccggcaa gccgcagtcg
     1381 caagaggacg ccgataagag cctgcagctt acgttatacg cgctggcggc ccgggatttg
     1441 ttgggcaaac gcgccgaccg tttgatcttt cacaatctcg aaaacaatac tccaatatgc
     1501 agcacccgga ccgatgccga aatagattcg gcgagattgc aagttgaaga agtcgcgcag
     1561 gccatcgcgc aggaacaatt cgcgcccaac ccgggccgcc actgcaatta ctgctcctac
     1621 cggaatctgt gcccagctac cgaaaagatt gtccccgaaa agttaacgca gcagaagaag
     1681 tcgtcgcgcc tgaactagtg tgatgacggg cgtctcgccc gtctggccta aaaactttgg
     1741 ggacgctcat cgcgtcccct ttgtcgttgc ctgttgcagt tggttagtgc ttcttgctgc
     1801 ttttcttctt tgctttcttt ttggttgcca ttgatctatt ctccctttcc attcttcatg
     1861 gaatttagca acgatgtttt gttgcaacta gttgatgtat agagtcaaca cgaaacgaag
     1921 tcaagagaaa aatgatcgaa acgcatgcat gcaatcctgt actaaagtca cgtgagtgac
     1981 tcgcacgcga aaaaatttct cgccgcatcg cttcttcgct ctcatccctc atcttttttc
     2041 gtgcgcgctc cgaatttgtg atcgctgtaa tcttctttgc aaagctttgc aaagcaaacg
     2101 cgatgccgcc ccgcgaaccg ccgatcacct ctccgacatc tataatgaaa gacgatttca
     2161 agcgatgatt gcttgacgcg gcgcatacga agagcgtcgc taaggagcgc aactatggcg
     2221 ggttctaacg atgttgtcat catctctgca tgccgcacac ccatcggaaa atttcaagga
     2281 agcctgagcg acctcaccgc tccaaagatt ggagcggtcg cagttcgcga ggcggtgaag
     2341 cgcgcgaatc ttaagcccga acaaatcgac gagtgcatca tgggcaatgt tgtctccgcc
     2401 ggcataggtc aaaatcccgc gcgccaggca gcgctcttcg gcggactcgc tcccgaagta
     2461 tgtgccatga caattaacaa ggtatgcggc tccggattga aagccgtagc gctggcagcg
     2521 caggcaatcg agacaggaaa cagttccatc gtcgtcgccg gcggcatgga gtcgatgacc
     2581 aacgctccat atctcctccc caacgcgcgc aaaggttatc gcctcggcaa cggacagctc
     2641 gtcgactcca tggtccacga cg
//
LOCUS       DATCBB010000399         1903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_434974, whole genome shotgun sequence.
ACCESSION   DATCBB010000399 DATCBB010000000
VERSION     DATCBB010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434974"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VII37_04505"
     CDS             complement(<1..301)
                     /locus_tag="VII37_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY5069018.1"
                     /translation="MKFKPLKRAPVIIAVLVLLFLCGVSLLRLDFFDQLERFTYDLRA
                     RAALKFPAAAATNLAFVAMDDSSIAAVKNGALGYKFGLYWPRQVYGRLVEELVAQG"
     gene            complement(375..1175)
                     /locus_tag="VII37_04510"
     CDS             complement(375..1175)
                     /locus_tag="VII37_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY5069019.1"
                     /translation="MTFAITASADDLKQGVATVVRVRGQASYTLGGTDDWHPLVAGKI
                     LQAGSTISTKPDSIVDVVLGKQVAMPQAQPVPDRVSPAADAPVRGIVGYKPSAEQNVV
                     RLSGGTTLKIDTLTVSDTGVDTVSDTELDLQAGRIFASVKKLSPTSKYLVKIPNGIAG
                     VRGTKFTLGADGSAGCIENSVWVSEVGADGKPVTVEVTQGTQYDPATGQSGPLPHQTL
                     KLLQEIAAAAGTSYYEVVSYAVNRANVVRVSPTAGFQPRLVLSSPTPQ"
     gene            complement(1309..1713)
                     /locus_tag="VII37_04515"
     CDS             complement(1309..1713)
                     /locus_tag="VII37_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069020.1"
                     /translation="MSEIAKFVNNGSASITPAMAGKILHHLPQWKLEFTQINAPKFPH
                     LVNQLEFLADAVEDAVEGAYKDLPYVAVAQSVFALLYAHKKMGIIPESILELGRADDS
                     SVVRAVLIQNEKAFATYAGSQGISWQKITSQP"
BASE COUNT          427 a          525 c          532 g          419 t
ORIGIN      
        1 cgccctgcgc cacaagttcc tcgacgagcc ggccataaac ctgccgcggc cagtaaagcc
       61 cgaacttgta gccgagcgcg ccatttttga cggcggcgat gctggaatca tccatcgcga
      121 cgaaggcgag attggtcgcg gcggcagcgg gaaacttcaa tgccgcccgc gcgcgtaaat
      181 cgtaggtgaa acgttcgagc tggtcaaaaa aatcaagccg cagcaggctt acaccgcaaa
      241 ggaacaggag gacgaggacg gcaatgatga cgggcgcacg ttttagcggt ttgaatttca
      301 acgtccgaat taaaacaaaa aaccagagca actcaaggct gctctggttt gttaaagcgt
      361 tttacagtgt atcatcactg gggcgttgga gatgacaaaa ccagccgggg ctgaaagcct
      421 gccgtcgggg agacgcgcac cacgttagcg cgattcaccg cataggaaac aacttcataa
      481 taactggtgc cggccgctgc cgcgatttct tgcagcagtt tgagggtttg gtgtggcagc
      541 ggtccgctct ggccggtggc gggatcatac tgggtgcctt gtgttacttc aaccgtgacc
      601 ggtttaccgt cggcaccgac ttctgaaacc cagacagaat tctcaatgca accggcagaa
      661 ccgtccgcac ccagggtgaa tttcgtgccg cgcacgccgg cgatgccgtt gggaatctta
      721 acgaggtact tcgacgtggg ggagagtttt ttgacgctcg caaatatgcg gccggcctgc
      781 aaatcaagtt cggtgtcact gaccgtgtcc acgccggtgt ctgaaaccgt caacgtgtca
      841 atcttcaaag tggtgccgcc cgagagcctg accacattct gctccgctga gggcttgtaa
      901 ccaacgatac cgcgcaccgg ggcatcggcc gccggcgaaa cccgatccgg caccggctgc
      961 gcctggggca ttgccacttg ctttcctaaa acgacatcca caatggagtc cggcttggtg
     1021 ctgatggttg aacccgcttg caggattttt cccgcaacca gcggatgcca gtcgtcggtg
     1081 ccgcccaacg tgtaactggc ctgacccctg acacgaacca cggtggccac gccctgtttt
     1141 aaatcatccg ccgaggcggt gatggcgaac gtcaaagccg ctccgcaaat tgcagcgcaa
     1201 aatatagttt taatattttt cataaggcag tttcttgaac tatctttctt gatttttaaa
     1261 ttaacagtta tgccagcggt tgtcaacgca ttaagtgacg cttcaaattc aaggctggct
     1321 ggtgattttt tgccagctga tgccctgcga cccggcgtaa gtggcgaagg ccttctcatt
     1381 ctggatcagc acggcacgca cgacgctcga atcgtccgcc cgacccagtt ctaaaatgct
     1441 ttccggaatg atgcccatct ttttgtgcgc gtagagcagc gcaaacaccg attgggcgac
     1501 cgcgacataa ggcaggtctt tgtaggcccc ttcgacggcg tcctcgacgg catcggccag
     1561 gaattccagc tgattgacca gatgcggaaa ttttggcgcg ttgatctggg taaactccag
     1621 tttccactgc ggcagatggt gcaggatttt tccggccatc gccggcgtga tgctcgcgga
     1681 gccgttgttg acgaattttg cgatttcgga catgacggca atttagagaa acccctgccg
     1741 ccgtcaaagt gaaatcgccg tgacgaatcg cgctttcatt tcgccggaaa ttcttttacg
     1801 aatagcgtgt gcaagatttg atcgccaaag ccggaacgct gctcgaagcg ctgccgtata
     1861 tccaaaagtt cagcggagcg acgttcgtcg tcaaatatgg cgg
//
LOCUS       DATCBB010000400         1214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_46074, whole genome shotgun sequence.
ACCESSION   DATCBB010000400 DATCBB010000000
VERSION     DATCBB010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46074"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VII37_04520"
     CDS             <1..527
                     /locus_tag="VII37_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEY5069021.1"
                     /translation="QYPSLNIRGLRSAYVGDQAQNVVPEKAIAAIDVRLVKGEDPDKK
                     FEQVLKHIRKQGFFVTSADPTKEERLAHPFIARVAKDPWNYPASRTSMELPVSQTLIK
                     VVQDATRGQTVIAPYLGGSVPMFIFENLQIPWIGVPIVNYDNHQHSSDENLRLGHLWR
                     GMEIYAAILADLNW"
     gene            complement(664..>1214)
                     /locus_tag="VII37_04525"
     CDS             complement(664..>1214)
                     /locus_tag="VII37_04525"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY5069022.1"
                     /translation="DNYDSFTYNLAQYLGELGCEVEVHRNDKISVDDIARKKPERIVI
                     SPGPCTPQEAGISVELIEELAGKFPILGVCLGHQAIGAAFGGKIKRAPALFHGKTSEI
                     HHDGKGVFRKLADPFTATRYHSLIVERKTLPRELMITAETDDGIIMGLRHRRYKIEGV
                     QFHPESVLTDSGKELLKNFLSL"
BASE COUNT          280 a          384 c          277 g          273 t
ORIGIN      
        1 tgcaatatcc ttctctaaat attcgcggcc tgcgcagcgc ttatgtcggc gaccaagcgc
       61 aaaacgtagt ccccgaaaaa gccatcgcag ccatcgatgt gcgtttggta aaaggggaag
      121 accccgacaa gaagttcgag caggtcctca agcatattcg caagcaaggt tttttcgtca
      181 ccagcgccga tcccacaaaa gaagagcgcc tcgctcaccc attcatagct cgcgtcgcga
      241 aagatccctg gaattacccc gcctcgcgca cctcgatgga attaccggtt tcccaaacgc
      301 tgatcaaagt cgtgcaggac gcgacccgcg gccaaactgt aatcgcgcct tatttaggcg
      361 gcagcgtccc catgttcatt ttcgaaaacc tgcaaatccc gtggatcggc gtacccatcg
      421 taaactacga caatcaccag cacagctccg acgaaaacct ccgtctcggc catctctggc
      481 gcggcatgga aatttatgcc gccatcctcg ccgatctgaa ttggtagtag cgctaaatca
      541 aatttattcg gtccacgccc tgcgacgtgg cagatttggg gtgccccagg ttcgcagttg
      601 agttatgaac ctgggtcttg aagttgactt ctccctctcg cactcgttaa cggccgcgtt
      661 gttctacaac gacaaaaaat tcttcagcaa ttcctttccc gaatcagtaa gcaccgattc
      721 cggatgaaac tgcactcctt caatcttgta ccgcctgtgc cgcaatccca tgatgatccc
      781 gtcatccgtc tccgcagtga tcatcagctc acgcggcaaa gtttttcgct ccacaatcag
      841 cgagtgataa cgcgtcgccg tgaatggatc cgccagcttc cgaaatacgc ccttcccatc
      901 gtgatgaatc tcgctggtct tgccgtggaa cagcgccggc gcgcgtttta tcttcccgcc
      961 aaaagccgcc ccaatggcct ggtgccccag gcacacacca agaatcggaa actttcccgc
     1021 cagctcctca atcagctcca cagaaatacc cgcttcctgc ggcgtgcagg ggcctggcga
     1081 aatcacgatg cgctccggtt ttttccgcgc aatatcgtcg acgctgatct tatcgttgcg
     1141 atgcacttcc acctcgcatc ccaactcgcc caaatactgc gccaggttgt aagtaaatga
     1201 gtcgtagttg tcga
//
LOCUS       DATCBB010000401         1015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_77630, whole genome shotgun sequence.
ACCESSION   DATCBB010000401 DATCBB010000000
VERSION     DATCBB010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77630"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VII37_04530"
     CDS             complement(<1..823)
                     /locus_tag="VII37_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5069023.1"
                     /translation="MARQRKLAESRADRTTREMAAIVEYSCDAIYSSRPDGIITSWNR
                     AAENLYGYTAEEAVGSPVARLAPPQRREEIERNLAILNQGGHVASYRTERMRKDGSLF
                     PVLLSVSPLRNARGEIVGTSAIARDISPEKQSEEAIRRSEKLATTGRLAASIAHEINN
                     PLEAVLNLLYLARHDSSHATKYLTMAEHEVGRVAQLAQQTLGFVRDNSSPEAMNPAGI
                     MDEILQLYSRKLEGRQIRVTRRYRNQLQINGYSGELRQLLANLLVNSVDAMAEKGS"
BASE COUNT          215 a          284 c          314 g          202 t
ORIGIN      
        1 gcgaaccttt ctcagccatg gcatcgacag aattcaccag aagattcgcc agcagttgcc
       61 gcaattcgcc ggaataacca ttgatctgaa gttggttgcg gtagcgccgc gtgacgcgaa
      121 tttgccggcc ctcaagtttt cgcgaataga gctgcagaat ttcgtccatg atgccggcgg
      181 gattcatcgc ttccggggag ctgttatcgc ggacgaagcc taaagtctgt tgcgccaact
      241 gcgcgacgcg gcccacttca tgctcggcca tcgtcaggta cttggtggcg tggctggagt
      301 catggcgggc caggtacagc aggttaagga cagcctccag ggggttgttg atctcgtgcg
      361 ctatggaagc ggcgaggcgt ccggttgtgg ccagcttctc gctgcggcgg atggcttcct
      421 cggactgctt ttcaggagaa atatcgcgcg cgatggccga ggtccccaca atttccccgc
      481 gggcgtttcg cagcggcgac accgaaagca ataccggaaa aagactgcca tccttccgca
      541 tgcgctctgt ccgataagaa gccacatgtc cgccttgatt caggatcgcg aggttgcgct
      601 cgatctcttc gcggcgctgc ggaggtgcga gtcgtgccac cggtgaacca acagcttcct
      661 cggcggtgta gccgtagaga ttttctgcgg cgcggttcca actcgtgata atgccgtcgg
      721 gccggctgct gtagatggcg tcgcaggagt attccacgat ggccgccatc tcacgtgtgg
      781 tgcgatcggc gcgcgattcc gccaacttcc tttggcgggc catgctcccg gcaaatacgg
      841 aaatggcgag aaatactgca agccgctcga ggtccgcgcc ggagcggacg gagaaacgga
      901 agaatggtga cacggcaaaa aaatcgaggc tcgcggttgc aagtattgag caaaacactg
      961 cggggccaaa accaaggaaa cgagctgtta acaacaccgc cgccaataaa agagt
//
LOCUS       DATCBB010000402         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_272905, whole genome shotgun sequence.
ACCESSION   DATCBB010000402 DATCBB010000000
VERSION     DATCBB010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272905"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..742
                     /gene="rplT"
                     /locus_tag="VII37_04535"
     CDS             362..742
                     /gene="rplT"
                     /locus_tag="VII37_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY5069024.1"
                     /translation="MARVKRGTKRRARRKKYLKRTKGFFLTKSKLYQSAQEAANRADR
                     YAFRDRRVKKRQYRRLWIQRIGAAARLNGITYGQLIHGLKAAGVDLDRKILADIAVKD
                     AAAFKLLAEQARAAAPPQKKKKSV"
     gene            897..2033
                     /gene="pheS"
                     /locus_tag="VII37_04540"
     CDS             897..2033
                     /gene="pheS"
                     /locus_tag="VII37_04540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY5069025.1"
                     /translation="MNAAENITAKKLADLGVTEPDQVAPLFAQVRSRFDEESGRVHDE
                     ESWKLFRDTWLGRKSGVLTQITDNWLKPAGPALKPAVGAALNQLRAHVEPKIEALHAS
                     IDSGGDAVSPRERVDLSLPGVIRPVGSRHLIRQVFQEIEDIFFSIGFSVAEGPEIETP
                     YYNFEALNIPEFHPVRDDMDTFYIDVPAGKQSGGSLLLRTHTSPVQIRTMEKTKPPVR
                     VIAPGKVYRRDNPDATHSFMFHQIEGLAVDSDITFCDFTGTIEYFVKQFFGSGVKTRF
                     RPSYFPFTEPSVEFDASCIFCNGTGSNSSGATCSKCKGSGWIELFGAGMVDPAVYGFV
                     NYDPKKVSGFAFGIGIDRLAMLKYGIDDIQAFFQNDVRFLRQFP"
     gene            2050..>2234
                     /locus_tag="VII37_04545"
     CDS             2050..>2234
                     /locus_tag="VII37_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069026.1"
                     /translation="MTFADAKNRFSNRVADYLRYRPHYPEGVLDFLRSQCHLSAAHTI
                     ADVGSGTGFLSELFLENG"
BASE COUNT          510 a          610 c          595 g          519 t
ORIGIN      
        1 catgcgcaag ctgaagaagc ttacgctggt gaatgccgcg gacaaggcta acgtgaagct
       61 cttgcttccg tacgcctaga tcgaatttgc ggtaaagact ttcagcctca aattatcgag
      121 ctgatgtagt agcaagaaaa agtcgggcgc aagcccgcgc cgttccgctg aaaagcgaaa
      181 tggcatcgcg ccaggcagcg cttcacgggt gcctgccggc cgataacgag ccgcataaag
      241 tgttcacctc acggtgaaca cacgcggctc agcagttcta ccggagacaa acccgttgcc
      301 ttacgcgtat ttttgcgttc gcgcagcccg ttgtctccca ttttattaag gagaaaccca
      361 gatggcacgc gtaaaacgcg gcactaaacg ccgtgcgcgg cgaaagaagt atctcaagcg
      421 cacaaaaggt ttttttctca ctaagagcaa gctgtaccaa tccgcgcagg aagccgccaa
      481 tcgcgccgac cgctacgcat tccgcgatcg ccgcgtaaaa aaacggcagt atcggcgcct
      541 gtggatccaa cgtatcggag ccgccgcgcg actgaacggc atcacctacg ggcagttgat
      601 tcacgggctg aaggccgctg gcgtggatct tgatcgcaag atattggccg acattgccgt
      661 aaaagatgcg gcggctttca agttgctcgc tgagcaagcc agagccgcgg ctccaccgca
      721 gaagaaaaag aaatccgtct agagcgtttc gcttgggctg gaacataagt agcggcggat
      781 accccaagac ccaggttcat acctcaagtg tgaacctggg gcaccccgcc tgacgatcat
      841 caggttcgga aattcacgaa cggtgataac gtttgtcgag tcaccgcaat caattcatga
      901 atgctgccga aaacatcacc gcgaaaaagc tcgctgatct cggcgttacc gaaccggatc
      961 aggttgcgcc tctcttcgcg caagtacggt cgcgattcga cgaagaatct gggcgcgttc
     1021 atgatgaaga atcgtggaag cttttccgcg atacctggtt agggcgcaag tccggcgttc
     1081 tcacgcaaat cactgacaac tggctaaaac cggcgggccc tgctcttaaa ccggcggtgg
     1141 gcgcggcgct aaatcaactg cgtgcgcacg ttgagccaaa aattgaggcg ttacacgctt
     1201 cgattgattc cggtggcgac gctgtcagcc cacgtgagcg tgtggatctg tcgcttcccg
     1261 gagtaatccg cccggttggc tcgcgccatt taattcggca ggtgtttcag gaaattgaag
     1321 atattttctt ttccatcggg ttttccgttg cggaagggcc ggaaatcgaa acgccttact
     1381 acaatttcga ggcgctcaac attccagaat ttcatccggt gcgggatgac atggacacgt
     1441 tctatatcga cgttcccgct ggcaagcaat ctggaggcag tctgctcctg cggacacata
     1501 cttctccagt gcaaatccgc actatggaaa aaaccaagcc cccggtgcgc gtaatcgcac
     1561 caggtaaggt ttatcgtcgc gacaatcccg acgccacgca ttcgttcatg tttcatcaga
     1621 tcgaaggcct ggccgttgat tccgatatta ctttctgtga tttcaccggc accattgaat
     1681 atttcgtgaa acagttcttc ggctctggcg taaaaactcg tttccgtccc agttattttc
     1741 cgttcacgga accctccgtg gaattcgatg cgtcgtgcat cttttgcaat ggcaccggtt
     1801 cgaattcctc cggtgcgaca tgctcgaagt gcaaaggctc gggctggata gaactcttcg
     1861 gtgcgggtat ggtcgaccct gcggtatatg gcttcgtgaa ttacgacccc aaaaaggtca
     1921 gcggctttgc cttcggcatt ggcatcgaca ggctggcgat gttgaagtac ggcatcgacg
     1981 acattcaggc attttttcag aacgatgtac ggtttttgcg gcaattcccg tgatgaggct
     2041 tctcgcgctg tgactttcgc tgacgctaaa aatcgttttt caaaccgcgt tgccgattat
     2101 ctgcgttatc gcccgcatta tcccgagggc gtgcttgatt ttctgcgctc gcaatgtcat
     2161 ctgtccgctg cgcacaccat cgctgacgtc ggttcgggaa ctggatttct atccgaactg
     2221 tttttagaaa atgg
//
LOCUS       DATCBB010000403         1127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_305360, whole genome shotgun sequence.
ACCESSION   DATCBB010000403 DATCBB010000000
VERSION     DATCBB010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305360"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..731
                     /locus_tag="VII37_04550"
     CDS             42..731
                     /locus_tag="VII37_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5069027.1"
                     /translation="MLESKKVSTNVYLRRIHNFALDMTWLPWPVLAKKCWPAVEFKEK
                     RGITLAEHLAIVARESNPERKAFYKLAWHLGASQSDLAFLEAENVDWDHHVISYKRMK
                     TKTVAIMRFDEDMVEILRDLPGTGPLFPYLRIVRAGDRATEFKQRCDGLGIKGVSLHS
                     YRYAWAERAKTAGYPERYAQVNLGHNSKAMTRAYSRKAEVEMPSLSEYERMRAKFAEM
                     SKTVEPVMASA"
     gene            752..1015
                     /locus_tag="VII37_04555"
     CDS             752..1015
                     /locus_tag="VII37_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069028.1"
                     /translation="MNPAMLLDPVFHFLDWSIAEWGVYLFLAFVWLCLILIAWIFGGG
                     LRRKFPNQPHVSAGIGIVIQSHTPPLPPTIIIHEHDPPDDGMD"
BASE COUNT          249 a          323 c          325 g          230 t
ORIGIN      
        1 atcagccgct tctggaaacg cgcacggaac attttctgcg cgtgcttgaa tccaaaaagg
       61 tttcaaccaa cgtctatctg cggcgcatcc acaacttcgc tctggacatg acttggttgc
      121 cgtggccggt gctggccaag aaatgctggc ctgcggtcga gttcaaggaa aagcgcggca
      181 tcacgctggc cgaacatctg gccatcgtcg cccgcgaatc caatcccgaa cgcaaggcgt
      241 tctacaaact ggcgtggcac ctgggcgcat cgcaatcaga cttggcattc ctcgaagcag
      301 agaatgttga ttgggaccac cacgtcatca gctacaagcg gatgaaaacc aaaacggttg
      361 ccatcatgcg ttttgacgag gacatggtgg aaatcttgcg cgacctgccg ggaaccggtc
      421 ccttatttcc gtatctgcgc atcgtccgcg cgggcgaccg cgccactgag ttcaagcaac
      481 gctgcgacgg gcttggcatc aaaggcgtgt cgttgcacag ctaccgctat gcgtgggcag
      541 aaagggcaaa aacggcgggt tatcctgaac gctatgcgca ggtgaacttg ggacacaaca
      601 gcaaggccat gacgcgggcc tattcgcgca aggccgaggt ggaaatgcct tcgttgagcg
      661 aatacgaacg gatgcgcgcc aagttcgcgg aaatgtccaa gaccgttgag ccagtgatgg
      721 ccagcgccta accggaggaa atcacatgaa cttgaatcct gccatgttgc ttgaccccgt
      781 gttccatttt ctcgattgga gcatcgcgga atggggagtt tatctgtttc tcgcgttcgt
      841 gtggctgtgc ctgattctga ttgcttggat attcggcggc gggttgcgcc ggaaattccc
      901 caatcaaccg cacgtcagcg ccggcatcgg cattgtcata cagtcacata caccaccgct
      961 gccaccaaca atcatcatcc acgaacatga cccgccggat gatggcatgg attaatccag
     1021 gctttctgtg aagtgtgacg atggggacgg gcgagcggcg gcgcggaagc tccgagcctc
     1081 ctgctcgccg tgccgccggt cgcaactgcg taacggactc gcccgtc
//
LOCUS       DATCBB010000404         1866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_143271, whole genome shotgun sequence.
ACCESSION   DATCBB010000404 DATCBB010000000
VERSION     DATCBB010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143271"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..615
                     /locus_tag="VII37_04560"
     CDS             58..615
                     /locus_tag="VII37_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069029.1"
                     /translation="MNESSNTKKSLILQTARQIGVQKWTPAEIDQLRRRLIAEHGEAG
                     KTGSDYIADVLEAAGLKLELTLQEEAEDQYEEEFEDLLHFKTLEDAEVSVMRLDELMR
                     KFRSQGENAAVERVLNVARLGKRRAEMISRNQKVEAPKRAEKEEIANWFRIWLENPDA
                     FFDWLDLRKQSPEFKSKFPQAGLVE"
     gene            612..1211
                     /locus_tag="VII37_04565"
     CDS             612..1211
                     /locus_tag="VII37_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069030.1"
                     /translation="MSSPGNALGSGALDLPPFSLDVEVLNLGCEVKAVGLELLETESR
                     EPVRGNKAAQIWSAAFPVLAGEEFYVLDFFSHLDRVRDFCSTHEIPFRDAAERCLVIP
                     KPTQDQLLDIFTRFEAETFGMRVGPSAETSDPAVEGELSKRGLDAYQSAYTRYTFCAV
                     CEPEDGWITLLSEKLWPSEVIRRVRPALQPFDVHIARPN"
     gene            1217..>1866
                     /locus_tag="VII37_04570"
     CDS             1217..>1866
                     /locus_tag="VII37_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069031.1"
                     /translation="MPAKRQPTQTTRHNLLLVSFGILALIFAGCGGQVSIIKNTPSAE
                     KPVVKDATREELLEKFNSTAASINSLNATVELKPTAGSKYSGIIEEYHEVKAFLLAER
                     PANIRVIGQAPVIGTTVFDMASDSQEFRVSIPSKNKFIVGPVALERASGKPIENLRPQ
                     HLLDALLWPEIRKQETVLFEEFNDETARYYVLTVLRGGYQTEILRKIWFDRSVLEVA"
BASE COUNT          469 a          572 c          455 g          370 t
ORIGIN      
        1 caacaataat gtgattaagt accgtcgtct tcttgccggg gagcaggctt cgaatcgatg
       61 aacgaatcgt ccaacacaaa aaagtcgctc atcctgcaaa cggcccgtca aatcggcgtg
      121 cagaagtgga cgcccgcgga aatcgaccag ctgcgccgac gcctcatcgc cgaacatggc
      181 gaagcaggca aaaccggcag cgactacatc gccgatgttc tggaagccgc cggcctgaaa
      241 ctggagctca ctctgcagga agaagccgaa gatcagtatg aggaagaatt cgaagacctg
      301 ctccatttca aaacattaga agacgccgaa gtcagcgtca tgcgcctgga tgaattaatg
      361 cgcaagtttc gctcgcaagg cgaaaacgcc gcggtcgaac gcgttctcaa cgtggcgcgt
      421 ctcggcaagc gccgagccga gatgatctcg cgcaatcaaa aagttgaagc tcccaagcgg
      481 gccgagaaag aagagatcgc caattggttc cgcatctggc ttgaaaatcc cgacgctttc
      541 ttcgattggc tagacctgcg caagcagtcc ccggaattca aaagcaaatt tccccaagca
      601 ggactggtcg aatgagttct cctggcaacg cgctgggcag cggcgctcta gatctaccgc
      661 cgttttccct ggatgtagaa gttctgaatc tcggctgcga agtaaaagct gtcggcctcg
      721 aattgcttga aacagagagc cgcgagccag ttagaggaaa taaagcagca caaatttggt
      781 ccgctgcttt cccagttctc gccggcgaag aattttacgt cttggatttt tttagccacc
      841 tcgaccgcgt ccgcgacttc tgcagtaccc acgaaattcc ttttcgcgat gccgctgagc
      901 gctgcctggt aattcccaaa cccacgcaag accagcttct cgatatcttt acccgcttcg
      961 aagcggaaac atttggaatg cgcgtcggcc ccagcgctga aacctccgat cccgctgtcg
     1021 aaggcgaact ctccaagcgc ggcctcgacg cctatcaatc cgcctatacc cgctacacgt
     1081 tttgcgcggt ctgtgaaccg gaagacggct ggatcactct actcagcgaa aaattatggc
     1141 ccagcgaagt aatccgccgt gtgcgcccgg cgcttcagcc ttttgacgtg cacatcgcgc
     1201 ggcccaatta aatctcgtgc cagcaaaacg ccagcccacc caaaccacgc gacacaattt
     1261 gctcctcgtt tcgttcggca tactcgccct catattcgcc ggatgcggcg gccaggtaag
     1321 catcatcaaa aacactccct ccgccgaaaa acccgtcgtc aaagacgcaa ctcgcgaaga
     1381 attgctggag aaattcaact ccaccgcagc ctccatcaat agcctcaacg ccaccgtcga
     1441 gctcaagccc accgcgggct ccaaatacag cggcataatc gaggaatatc acgaagtaaa
     1501 agctttcctt ctcgccgagc gcccagcaaa catccgcgtt atcggccaag ccccggtaat
     1561 cggcaccacc gttttcgata tggccagcga cagccaggaa tttcgcgtct ctatcccctc
     1621 caaaaataaa ttcattgttg gcccggtagc tctcgaacgc gccagcggca agcccatcga
     1681 aaatctacgc ccgcaacatt tgctggacgc gcttctgtgg cccgaaattc gcaagcagga
     1741 aaccgtgcta ttcgaggaat tcaacgacga aaccgctcgc tattacgtgc tcaccgttct
     1801 gcgcggtggc tatcaaacgg aaatcctgcg caaaatctgg tttgatcgct ccgttctgga
     1861 agtagc
//
LOCUS       DATCBB010000405         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_369680, whole genome shotgun sequence.
ACCESSION   DATCBB010000405 DATCBB010000000
VERSION     DATCBB010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369680"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VII37_04575"
     CDS             complement(<1..1069)
                     /locus_tag="VII37_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5069032.1"
                     /translation="MADALTSPGASSTNSTPSRLFSWPRDTTAAERQTLVAGGLGWML
                     DAMDVMLYSLVLAFLMREFAMDTRTAGLLNSLTLLASAIGGLFFGWIADRIGRTRALM
                     ASIGMYSVASAACGFSHSIPELAAFRFLLGLGMGGEWTAAAALIAETWRPEHRGKALG
                     LMQSSYAIGEAIAALVVAVVLPHFGWRAVFFVGILPALLVFWIRRNVPEPSVWKDRAQ
                     AERPGFFRLLFGKGVFRVGLLATAMNAFGMFGYWGLFTWIPAYLSLPVSQGGRGLSLV
                     KTTTFFLVLCLGKWLGYALFGFFADAFGRRKPYFIYLLIAAALTPIYGSAKSAFWLLL
                     LGPFVAFFGTGFFSGYAAIASE"
     gene            1317..1679
                     /locus_tag="VII37_04580"
     CDS             1317..1679
                     /locus_tag="VII37_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY5069033.1"
                     /translation="MGTAPAASSEGLAENVAGLLCYVLGWLTGIIFILIDKRPFVRFH
                     AAQSIVVFGALTVLRIGLGIIMGIGGFVGFGLWALISMVLGLVGVILWIFLMIKAYQH
                     ELFRVPIAAPIADGIAGK"
     gene            1990..>2288
                     /locus_tag="VII37_04585"
     CDS             1990..>2288
                     /locus_tag="VII37_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069034.1"
                     /translation="MQPWTDGLFDGDENVIVDFKYSNSPKVTWWFDHHQSAFLTPADA
                     ENFRKHPGPKMFYEPEYKSCTKLIANVSKEKFGFDTTPLNDLIHWGDIIDGAQYPT"
BASE COUNT          573 a          657 c          582 g          476 t
ORIGIN      
        1 tttcactcgc tatggcggca tagcccgaga aaaatcccgt tccaaaaaaa gccacaaacg
       61 gcccgagcaa cagcagccaa aaagcgctct tcgccgagcc ataaataggc gtcagcgcgg
      121 cggcaatcag caaataaata aaatacggtt tacggcggcc aaacgcatcc gcgaaaaacc
      181 cgaacaacgc ataccccagc catttcccca aacacaaaac caggaaaaat gtagtcgtct
      241 tgaccaggct cagccctcgt ccgccttgag aaacgggcaa tgataagtaa gccgggatcc
      301 aggtgaacag tccccagtag ccaaacatcc cgaaggcgtt catcgccgtc gccaggagtc
      361 ccacgcgaaa aactcctttg ccaaataaca gccggaagaa tccaggtcgc tccgcttgtg
      421 cgcgatcttt ccacacagat ggctccggca cattgcgtcg aatccaaaag accagtagcg
      481 ctggtagaat tcccacgaaa aataccgctc gccacccaaa gtgcggcaat acgacagcaa
      541 cgaccagcgc cgcgatagct tcgccaatgg cataggacga ttgcatcaga cccaacgcct
      601 tgccgcgatg ttcgggccgc cacgtttcgg ctatcagcgc ggcagcagcg gtccattcgc
      661 cacccatacc cagccctaac aaaaaccgga atgccgccaa ctccggaatc gaatgcgaga
      721 atccgcatgc agcgctggcc actgagtaca tcccaataga ggccattagc gcgcgagtgc
      781 gcccaatgcg gtcggcgatc cagccaaaaa acagtccacc aatggcagag gcgagcaggg
      841 tcagtgaatt gagcaagccg gccgtacgcg tgtccattgc gaactcgcgc attaaaaaag
      901 cgagcaccag ggagtacaac atcacgtcca tggcatcgag catccagcca agaccgcccg
      961 cgacaagcgt ctggcgctcg gcagccgtcg tgtcacgcgg ccaactaaaa aggcgagagg
     1021 gtgtggaatt tgtggacgaa gccccggggg atgtcaacgc gtcggccata aggtccagcg
     1081 ctcagaaaaa gggccgtcag ccaaaagaag gtctccataa actgagagcc aaccgcaaaa
     1141 ttgccgcctg ccaggataaa acattgcgct gtgggccttc tttttgttac gctccggcgc
     1201 attatatccc ctcggaggtc ttgcaaatgg cgttttgtaa agcatgtgga actgaattag
     1261 gcggcgcggc attctgtccc aagtgcggag ccagccaaac ggcatccgca gttcccttgg
     1321 gcacagcgcc ggcggcaagc agcgaaggtc tcgctgaaaa tgtcgcgggg ctattgtgct
     1381 acgtgctcgg atggctaacc ggaatcatat ttatattgat cgacaaacgg cccttcgtaa
     1441 gatttcacgc cgcgcagtcc atcgtggtat tcggcgcttt gacggtcctg aggataggtc
     1501 tcggcatcat catgggtatc ggcggctttg ttggattcgg gctctgggcg ttgatttcca
     1561 tggtgctggg cctggtcgga gtcattttgt ggattttctt gatgatcaaa gcctatcagc
     1621 atgaactatt ccgcgtgcca attgccgcgc ctattgcgga tggaatcgcc ggtaaataat
     1681 cagaccgtgt ttggaaaatc acccgggggg tcgctctctc atgagcgact cttcccccgc
     1741 gttgtataac gtgcgtttga tttgtgcttg ctattgactt tcccgttggc tcccaccatt
     1801 gtaaaaagct tcatttaacg ttgcattgag cctagaaagg catctgccag gtttatgacc
     1861 cgagtgcgcc tgtgtttcca tgatcgttgt tttgatggca ccgcctccgc cgcccttttt
     1921 taccgctttt atcgcgagcg ctttaatgcg caagcgcagt tcgaattaac tggcatgacc
     1981 caccgcgcca tgcagccctg gacagacggc ctttttgacg gtgacgaaaa cgtgattgtg
     2041 gacttcaaat attcgaactc gcccaaagtc acctggtggt tcgaccatca tcagagcgcc
     2101 ttcctgactc ccgcggacgc tgaaaatttt cgtaaacatc ctggaccaaa aatgttttac
     2161 gagccggaat acaaatcctg caccaagttg atcgccaatg tttctaaaga aaaatttggc
     2221 ttcgacacca ctcctctcaa cgatttaatt cattggggcg acataattga tggcgcgcaa
     2281 taccccac
//
LOCUS       DATCBB010000406         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_435066, whole genome shotgun sequence.
ACCESSION   DATCBB010000406 DATCBB010000000
VERSION     DATCBB010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435066"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VII37_04590"
     CDS             <1..428
                     /locus_tag="VII37_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069035.1"
                     /translation="VSKIRAYLNKWRPLRSGLPAVVNELTALGMPAGAKFDAIVEQIF
                     GMQLTGRGKTPEEREKILRKLSGIKEPPKKKEKEKKTKAAEKSHAAMAMGSAAKQKHA
                     DAETEARRAGKAGAKGAHKGEPARQAAGRASGKKTHARK"
     gene            519..>2725
                     /gene="dnaE"
                     /locus_tag="VII37_04595"
     CDS             519..>2725
                     /gene="dnaE"
                     /locus_tag="VII37_04595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5069036.1"
                     /translation="MGQPQFVHLHVHTDFSLLDGACETGELLDEASRQKMPAVAITDH
                     GNLFAAANFFNEASKRDVKPIVGCEVYVARGSRHERGEKTNGANGQERGDGEPAGRGS
                     NHLVLLCENQEGYKNLIKLVSSGFLEGFYYKPRIDYDLLAKHSKGLIALSACLRGVVT
                     EAVVEERYDQARENAYRLRDVFGKGNFFLEIQDQGLDIERTVNPHLVRLSKESGIPLV
                     ATNDCHYLKHSDAHAQEVLLCIQTGKTMSDAHRMKFATDQFYFKSAAEMAQVFGELPE
                     ALSRTVDIAARCNVKIERVSNPFPEFKVPDGHTPGSYFEMVVREGFATRVPYLERLAK
                     QGLLRNSLAEYERRLSFEIEMIQKMRYEGYFLIVWDFIHYARGQSVPVGPGRGSAAGS
                     LVSYALRITDVDPLQYNLIFERFLNPERVSMPDIDIDFCMRRRGEVIDYVTQKYGREN
                     VSQIITFGTMAAKAAVKDVGRAMDIPYMEVDRLAKLIPTTLGIELETALKEAPQLRAA
                     VDADQRLKDLMAVALRLEGLARHASTHAAGVVISARPLTDIVPIYKTNRDEITTQYDM
                     NALERIGLLKMDFLGLTTLTVLNDTVNMIERNRGAKVDIDNLLLDDTETYKLFARGDT
                     TAIFQFESHGMRDILRRYQPTRIEDLTALNALYRPGPIQGGMIDDFINRKHGKTKVSY
                     ELPQLQDILEETYGVIVYQEQVMQIANKLASFSLGEADILRRAMGKKKKEEMAA"
BASE COUNT          677 a          718 c          768 g          562 t
ORIGIN      
        1 cggtctccaa aatccgcgcg tatctgaaca aatggcgccc gctacgttcc gggctgcctg
       61 cggtggtcaa cgaattgact gctttgggaa tgccggctgg tgcgaaattt gacgcgatcg
      121 ttgagcagat tttcggcatg cagttaactg gaaggggcaa gacgccggaa gaacgagaaa
      181 aaattctccg caagctttcg ggtatcaagg aaccgcccaa aaagaaagaa aaagagaaga
      241 agaccaaagc agcggagaaa tctcacgcag cgatggccat gggcagtgct gcaaaacaga
      301 aacacgcgga tgccgaaaca gaggctagaa gggctggaaa agcgggagcc aagggcgctc
      361 acaagggtga acccgcgcga caagccgccg gccgtgcgtc cggcaagaag actcacgcgc
      421 gcaaatagtt cccggcgggc tggaaaagaa ttttggtggc agagttttca acaacagcca
      481 ttcgcaatta ttcgcgcaga gatttagaat cgaggtccat gggtcagccg cagtttgttc
      541 atcttcacgt tcacaccgat ttcagcctgt tggatggggc ttgcgaaact ggcgagttgc
      601 ttgacgaagc ttctcgccaa aaaatgccgg cggtggccat caccgatcat ggcaatcttt
      661 tcgccgcagc gaatttcttc aatgaagcca gcaagcgcga cgtcaagccc atcgtcggtt
      721 gcgaagtgta tgtggcgcgc ggtagccgcc atgagcgcgg ggaaaaaacc aacggcgcga
      781 acgggcaaga acgcggggat ggtgaacccg ctgggcgcgg cagtaatcac ctcgttctgc
      841 tttgcgaaaa tcaggaagga tataaaaacc tcatcaagtt ggtttcttcc ggtttcctcg
      901 aaggctttta ttacaagccg cgtatcgact atgacctgct ggccaagcac agcaaaggat
      961 taatcgcgct ttccgcgtgc ttgcgcggcg tggttaccga agcggtggtg gaagagcgat
     1021 acgatcaggc gcgcgaaaat gcttaccggc ttcgcgatgt gtttggcaag ggcaatttct
     1081 ttttggagat tcaggatcag gggctggata tcgagcgtac ggtcaatccg catctcgttc
     1141 gcctctcgaa agaatccggc atccctctag ttgccaccaa cgactgccac tatctgaaac
     1201 attccgacgc gcacgctcag gaggttttgt tgtgcatcca gaccggcaag accatgagcg
     1261 atgcgcatcg catgaaattt gccaccgatc agttttattt caagagcgcc gcggaaatgg
     1321 cgcaggtatt tggtgaattg cccgaggccc tgagtcgcac agtggatatc gccgcacgtt
     1381 gcaacgtgaa gattgagcga gtctccaatc catttcctga attcaaggtt cctgacggcc
     1441 atacccccgg gagttatttc gagatggttg tgcgtgaagg cttcgccacg cgtgttccgt
     1501 acctggagcg cctcgcgaaa caaggtctgc tgcgaaatag cctcgcggaa tatgagcgcc
     1561 gcttgtcttt tgagatcgag atgatccaga agatgcgtta cgaaggctat ttcctgatcg
     1621 tgtgggattt tattcattac gcgcgcggtc agagcgttcc ggtcgggccg ggccgcgggt
     1681 cggcagccgg cagtctggtt agctacgcgt tgcgcattac agacgtcgat ccgttgcagt
     1741 acaacctgat ttttgagcgc ttcctgaatc ccgaacgcgt gtctatgccg gatatcgata
     1801 tagatttctg catgcggcgc cgcggcgaag tgatcgatta cgtgacgcaa aaatacggac
     1861 gcgaaaatgt gtcgcagatc atcaccttcg gcaccatggc tgcaaaggcg gcagtgaaag
     1921 atgttggtcg cgcaatggat attccgtaca tggaagttga tcgcctagca aagttaattc
     1981 ccaccaccct gggcatcgaa ctggagaccg cacttaaaga ggcgccgcaa ctgagggctg
     2041 ccgttgacgc tgaccaaagg ctgaaagatc taatggccgt ggcgttacgc cttgaaggcc
     2101 tggcccgcca tgcttcgacg cacgccgcgg gggtggtaat ttccgcgagg ccacttacgg
     2161 acatcgtccc tatctacaag acgaatcgcg acgaaataac cacgcagtac gacatgaatg
     2221 cgctcgagcg catcggcctg ctgaaaatgg atttccttgg tctcacgacg ctcacggtgc
     2281 taaacgatac cgtgaacatg atcgagagga atcgcggcgc caaagtcgat atcgacaatc
     2341 ttttgctcga cgacaccgag acttacaaac tttttgcacg cggcgacacc accgccatct
     2401 tccagtttga gtcccacggc atgcgtgaca ttctgcgccg ctaccagcct acgcgcatcg
     2461 aggaccttac cgcgctcaac gcgctctacc gccccggccc gatccagggc ggcatgatcg
     2521 acgattttat aaatcgcaag catggcaaga cgaaagtcag ttacgaatta ccgcaactgc
     2581 aagacatcct ggaagaaaca tacggcgtaa ttgtgtatca agaacaagtg atgcaaatcg
     2641 ccaacaagct ggccagtttt tctctcggcg aagcggacat actccgtcgc gccatgggaa
     2701 aaaagaaaaa agaagaaatg gccgc
//
LOCUS       DATCBB010000407         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_239635, whole genome shotgun sequence.
ACCESSION   DATCBB010000407 DATCBB010000000
VERSION     DATCBB010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239635"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VII37_04600"
     CDS             <1..574
                     /locus_tag="VII37_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069037.1"
                     /translation="VRKESAPLVADTLTAAGLPVQASVFARGTISCTGSEFCKLALTE
                     TKSFARWLTEELEERLPAFEEQLKLHITGCPNSCGQHWIADIGIEGKKIKHNGKMVDA
                     YYFCVGGSVGQFAGIARPVGYRCTADSVPDAIERLLEGFNTRREGNENLRQFFGRHTN
                     EELRALLAGTFVGAVERDVALGPVPHGVEG"
     gene            588..1994
                     /gene="cysG"
                     /locus_tag="VII37_04605"
     CDS             588..1994
                     /gene="cysG"
                     /locus_tag="VII37_04605"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEY5069038.1"
                     /translation="MSLFPIFVKLQGRPVVVVGGGNIAAGKIPGLLQAQARVRVIAPQ
                     INSQVSAWANTGEIQWSATEFAPADVQDAALVITATSIPAVNAAVFQAAEAAGILCNA
                     VDDIANCHFYYGSIVQRGDLQIAISTNGKSPALAQRLRKELEQQYPAEYAAWLDSLGD
                     AREKLRASSDPRENFEENKAILHRLASPEMFSRFVRSQARESAPTKSGHRAPGTVYLV
                     GAGPGDPELLTVKATRLLAAADIVLYDSLVSAEILALISLRAEIVDVGKRAGRKLLTQ
                     DEINSLLVSYAESRTTVVRLKGGDPSMFGRAGEELEALRGANIPYEVVPGISAALGAA
                     AAAGISLTDRRVASQVLFSTFSRSPEHGQLKWSQLTADTTLVLYMPGSDYSEVAERLQ
                     NSGLPTDLPCVIVSRATQAEQQVRWSTVGSLAAEEKLPAPALFIVGRVASHQITEFSK
                     TFWTSHEVEMPQQVKSIA"
BASE COUNT          444 a          618 c          554 g          400 t
ORIGIN      
        1 cgtccgcaaa gaaagcgccc cacttgtcgc ggacacgctt accgccgcgg gcttgcccgt
       61 tcaagcttct gtctttgcac gcggcacaat ttcctgcact ggctccgaat tttgcaagct
      121 ggcgctcacc gaaaccaaaa gcttcgcgcg ctggctaacc gaggaactcg aagagcgttt
      181 gcctgccttc gaagagcaac tcaaattgca tatcaccgga tgcccgaaca gttgcggcca
      241 gcattggatc gctgacatcg gtatcgaggg caagaaaatc aagcacaacg gcaaaatggt
      301 cgacgcctac tatttctgcg ttggcggcag cgtaggacag ttcgcaggca tcgcgcgccc
      361 cgttggttat cgctgcacag cggactccgt gcccgacgcc atcgaaagac tcctcgaagg
      421 tttcaacacg cggcgcgaag gaaacgaaaa tctgcgccag ttttttggcc gccacactaa
      481 cgaagagctg cgcgcgctgt tggccggcac tttcgttggc gccgtcgagc gcgatgtcgc
      541 gctaggacct gtgccgcacg gcgtggaagg atgaggccga gaaaaccatg agtctttttc
      601 cgatatttgt gaaactgcaa ggccggcccg tcgtggtcgt cggaggtggc aacatcgccg
      661 ccggcaagat ccccggtctt ttacaagctc aggcgcgcgt tcgggtcata gccccgcaaa
      721 ttaattcgca ggtttccgcc tgggccaaca ccggcgaaat tcagtggtct gcaacggaat
      781 ttgcgcccgc cgacgtgcaa gacgccgccc tggtgatcac cgcaacttct attccagcgg
      841 tgaacgccgc agtgtttcag gccgcggaag cggctggcat tttatgcaac gccgtcgatg
      901 acatcgccaa ctgccatttc tattacggct cgatcgtgca gcgcggcgat ctacaaattg
      961 ccatctccac gaacgggaag agtcccgctc tcgcacaacg tttgcgcaag gaattggagc
     1021 agcaataccc cgcggaatat gcggcgtggc tggattccct gggcgacgct cgcgagaaac
     1081 tgcgcgccag ctcggatccg agagaaaact tcgaagaaaa taaagccatc cttcatcgcc
     1141 ttgcgagccc ggaaatgttt tctcgttttg ttcgttcgca agctcgggaa tcggccccaa
     1201 cgaaatcggg gcatcgtgct cctggcaccg tttatttagt tggtgcgggt cccggcgatc
     1261 cggaattgct caccgtaaag gcaacgcgtc tcctggccgc tgccgacata gtcctgtacg
     1321 actcactcgt cagcgcagaa attttagccc tgatttcgct gcgcgccgaa atcgtcgacg
     1381 tcggcaagcg cgccggtcgc aaactcctca cgcaggacga aatcaattcg ctgttagtct
     1441 cttacgccga aagcagaacc acggttgtgc gcctcaaagg cggcgatcca tccatgttcg
     1501 gtcgcgccgg cgaagaatta gaagcgctgc gcggcgcgaa tattccatac gaagtggttc
     1561 ccggcatcag cgccgcgttg ggagccgccg cggccgccgg aatctcgctc accgaccgcc
     1621 gcgtagcctc gcaagtgctc ttcagcacat tttctcgcag tcctgagcac ggccaactca
     1681 aatggagcca gctaaccgcc gacaccacgt tggtgcttta catgcccggc tcggactaca
     1741 gcgaagttgc cgaacgcttg cagaacagcg gcctgccaac cgatttgcct tgcgtgatcg
     1801 tctcgcgcgc cactcaggcc gaacaacaag tgcgttggtc caccgtcggc agcctcgctg
     1861 cggaagaaaa acttcccgcg ccagcacttt ttattgtggg ccgcgtcgca tcgcaccaaa
     1921 tcacggaatt cagcaaaact ttctggactt cgcacgaggt agagatgcct cagcaagtga
     1981 aatccatcgc ctaaaaaaat attggagaaa aaacaa
//
LOCUS       DATCBB010000408         1006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_207882, whole genome shotgun sequence.
ACCESSION   DATCBB010000408 DATCBB010000000
VERSION     DATCBB010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207882"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VII37_04610"
     CDS             complement(<1..958)
                     /locus_tag="VII37_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069039.1"
                     /translation="MAGGRSLLDAAISLQQERIRAAADEAFAESVSRFKDNLGGAEQL
                     LDETARSVTGRNIAEMEEKLSDIKHQAIQDVFKSAEWYEKKAQTHIQNVTEKAVEQAS
                     SQLKDKAGELSSVFTSELDSSSRNFVGRTQSQMEDTVRDAFERARTLFAEASDTTAAA
                     FTDEVQRTGRHELRGFGEELQKSVEQARAELDSAQRELSQKISSEQAEFLRRFQEAMD
                     KALESGVKDANEKVEASFSPLIDSWKTMTDRHQGDLRNVYAKISEQATEQHRTRLENV
                     SNQWMLATVTALDHQSREIVADISANAEEKLRETCTQVFENIG"
BASE COUNT          190 a          302 c          248 g          266 t
ORIGIN      
        1 cgccgatatt ctcaaaaacc tgagtgcagg tttcgcgtaa tttttcttcc gcgttcgccg
       61 aaatatcggc aacgatctca cgcgattgat gatccagtgc tgtcacggtg gctagcatcc
      121 actgattgga aacattttcg agtcgcgtgc ggtgttgttc ggtagcctgc tcgctgattt
      181 tggcgtacac gttgcgcagg tcgccctggt gccggtcggt catcgttttc caggaatcaa
      241 tgagcggcga gaaactggcc tcaactttct cattggcatc cttgacgcca gactccaaag
      301 ccttatccat ggcttcctgg aaacggcgca aaaactctgc ctgttccgag ctaattttct
      361 gcgacagttc ccgttgcgcg ctgtcgagtt ccgcgcgagc ctgttccaca ctcttctgca
      421 attcctcacc gaatccccgg agctcatgcc tcccggtgcg ctgaacttcg tctgtaaacg
      481 ctgccgccgt ggtatccgag gcttcggcaa aaagcgttcg cgcgcgctca aaggcgtctc
      541 gcacggtgtc ttccatctgc gactgggtac ggcccacaaa atttcggctg gaactatcca
      601 actcgctagt gaacacactg gaaagctcgc cagctttatc tttcagttgg gaactggcct
      661 gctccacggc tttttccgtc acgttctgga tatgcgtttg ggccttcttc tcataccatt
      721 ccgcggattt gaaaacatcc tgaatagcct gatgcttgat gtcgcttaac ttctcttcca
      781 tttcggcaat gtttcgaccg gtaaccgaac gcgcggtttc atccagcaat tgttccgcgc
      841 ctccgaggtt atctttgaac ctggaaactg actccgcaaa agcttcatcc gccgccgctc
      901 tgatgcgctc ctgctgcagg gatattgcgg catccaagag cgatcggccg cccgccatgc
      961 gctgaagcga ttcttccgtg ttttgcaagt gcggcgcaac cattcc
//
LOCUS       DATCBB010000409         1269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_77850, whole genome shotgun sequence.
ACCESSION   DATCBB010000409 DATCBB010000000
VERSION     DATCBB010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77850"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VII37_04615"
     CDS             complement(<1..513)
                     /locus_tag="VII37_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5069040.1"
                     /translation="MPKSGSPDSEMAPDPQSTPSWKALFRIPGFPFFFVAMMISLFGT
                     GMNFAGVTWYVLGVTHSTVKVSWIVILVTLPGLVVPPFGGVLIDRVDRRYLGITIDAT
                     RAVIVLGTAALAYAGRLAMWELYSMVLLLGVGFAIYWSTTNVLIQELVPQDKLITANA
                     TVLIAVQGGMA"
     gene            781..>1269
                     /locus_tag="VII37_04620"
     CDS             781..>1269
                     /locus_tag="VII37_04620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5069041.1"
                     /translation="MLTEQLQPGQRFEYFRIEALVARTCMTTIYRATDLRTGQLVAIK
                     IPHPELECDPAFYSRFQREIAIGKKLKHPSVVQTVDGGDMDQLCIVMEWVDGRFLREI
                     LTKEGKISAERAARIAFGVCEALEYIHSLGVVHRDLKPANVFLRARDANVTVKLVDFG
                     IAK"
BASE COUNT          309 a          376 c          325 g          259 t
ORIGIN      
        1 cgccattccg ccttgcacgg caatcaacac ggtggcattg gccgttatca atttatcctg
       61 cggtacgagc tcctggatca gcacatttgt agtggaccaa tagatcgcga atccaacacc
      121 caacagcaaa accatcgagt acagttccca catcgccaga cgccccgcat aggccagcgc
      181 ggccgtgccc aggacgatca ctgcgcgcgt tgcgtcgatc gtgattccca aatagcgccg
      241 gtccacgcga tcgatgagca ctccaccgaa tggaggcacc accagccccg gcagtgtcac
      301 caggatcacg atccagctga ccttcacagt ggaatgagtc acgcccagaa cgtaccaggt
      361 cacgccggca aaattcatcc ccgtcccaaa cagcgagatc atcatcgcca caaagaaaaa
      421 ggggaatcct ggtatgcgaa aaagcgcttt ccaactggga gtcgattgag ggtctggtgc
      481 catttcagaa tcgggagagc cactcttcgg catctgagcc aagtttagcg gcaatgtggt
      541 accaggtcca agaaaatgaa tccccatgca acgaaatcat tccatcaggg tgctttgcct
      601 ggttcactcc ggaactagct tcgccatcgc acccgatgcg gcaaccttta acttccagat
      661 tccaatatgg ttgctgttat ttatacgcat tgtatgaatt gtttatgctt cctttagagg
      721 caaaaagtta ttttgcctgc tagcaaccgt gtgccaattg gcgcaggaag gatacggcaa
      781 atgttgacgg agcaacttca gccgggccag cgcttcgagt atttccgcat cgaagctctc
      841 gtcgcgcgca cctgcatgac caccatctat cgcgccacgg atctgcgaac cggccaactc
      901 gtagcgatca aaatcccgca ccccgaactc gaatgcgatc ctgcgttcta tagccgcttc
      961 caacgcgaga tcgccatcgg caagaagctc aagcatccga gcgttgtgca aacggttgac
     1021 ggcggcgata tggatcagct ctgcatcgtg atggaatggg ttgacgggcg tttcttgcgc
     1081 gaaattctca ccaaggaagg aaaaatttcc gccgaacgcg cggcgcgcat cgcattcgga
     1141 gtctgtgaag ctcttgaata cattcacagc ctgggcgtcg tgcaccgcga cctcaaaccg
     1201 gcgaacgtgt tcctgcgagc gcgcgacgcg aacgtgacgg tgaagctcgt cgacttcggc
     1261 atcgccaag
//
LOCUS       DATCBB010000410         1401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_305664, whole genome shotgun sequence.
ACCESSION   DATCBB010000410 DATCBB010000000
VERSION     DATCBB010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305664"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /gene="murF"
                     /locus_tag="VII37_04625"
     CDS             <1..1332
                     /gene="murF"
                     /locus_tag="VII37_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY5069042.1"
                     /translation="NSRTVQPSELFFAVKGERLDGHDFVDHAISRGALAAVVQKAQVA
                     RYPKSAPLLLVDDTLVALQTLATAVRKKWGKTAIGVTGSMGKTTTKEALGHLLSIKYR
                     VHRTKGNFNNHFGLPLGLLTLEPEYDVAVVEMGMSNAGEISALARIALPNQGVVTNVA
                     PVHLESFDSLDGIARAKYELIEALPSGGTAVLNGDDEYVSQFGRNFKGKVIFFGLKPT
                     ADVRAENIEMVGTEGTRFDLISRDVRQLVQSPLLGKHNVYNVLAAAAVALEHGITPSE
                     IAAALPSLEPADKRGQVLQVGNVTVLYDCYNSSPKALMAAVDTLAAMPGARRIVVAGE
                     MLELGAGGEELHRECGRHIAEKVAGGQENKLDFLLGVRGLAEPMVEAAREAGMKAEFV
                     ATPEEAGEWLARETREGDVVLLKASRGVKLEKALEKWKEKRGSPNASGAGK"
BASE COUNT          309 a          335 c          469 g          288 t
ORIGIN      
        1 aattcccgca cggtgcaacc tagcgaactt ttttttgcgg tcaagggcga acgccttgat
       61 gggcacgact ttgtggacca cgccataagc cgaggcgcac ttgctgcagt tgtgcagaaa
      121 gcgcaggtag cgcgatatcc aaaatctgct cccctgttgc tggttgacga tactctcgtc
      181 gcgctgcaaa cgctggcgac agcggtgcga aaaaagtggg gcaagacagc gatcggcgtc
      241 accgggtcga tgggcaagac cactactaaa gaagcgctgg ggcatttgct ctccattaaa
      301 tatcgggtgc atcgcacgaa gggaaatttc aataatcact ttgggctgcc gcttgggctg
      361 ttgacactgg aacctgaata tgatgttgcg gtggtggaga tgggcatgtc caacgcggga
      421 gagatttccg cgcttgctcg cattgcgctg cccaatcagg gtgtggttac gaacgtagcg
      481 ccggtgcatc tggagagctt tgactcactc gacggaatcg cgcgcgccaa gtatgagctg
      541 atcgaggccc ttcccagcgg cggcaccgcc gtcttgaacg gcgacgatga atatgtgagc
      601 cagttcgggc gcaactttaa ggggaaagtt attttttttg ggttgaagcc aactgcggat
      661 gtgcgagcgg aaaatatcga gatggtggga acagaaggga cgcgcttcga tctgatcagc
      721 cgcgatgttc gccagttagt gcagagtccg ttgctgggaa agcacaacgt gtataacgtg
      781 ctggcggcgg ctgccgtcgc tctggagcat ggcattacac cgtcggagat cgccgccgcg
      841 ctgccgtcgc ttgagcctgc cgataagcgc gggcaggtcc tccaagtggg taacgttacg
      901 gtgttgtacg attgctacaa ttcaagtccg aaggcgttga tggccgccgt ggatacgcta
      961 gctgccatgc ctggcgcgcg ccggattgtt gtcgctggcg agatgctgga acttggcgcg
     1021 ggcggcgaag agttacaccg cgagtgcgga cgtcacattg cggagaaagt ggcgggaggc
     1081 caggaaaata aactcgactt tctgctgggc gtacgcggat tggctgagcc aatggtggag
     1141 gcggcgcggg aagcaggaat gaaagcggaa ttcgttgcga cccctgaaga agcgggtgag
     1201 tggctggcgc gcgaaacgcg ggaaggcgat gttgtattgc taaaagcatc tcgcggcgtg
     1261 aaactggaaa aggcgctgga gaagtggaag gaaaagcgcg gttctcccaa cgcctcaggc
     1321 gcggggaaat aaaaattgag agctgacagc tgagagctga cagctgttgt cggaggcgga
     1381 ttgctttact ggctgctcta t
//
LOCUS       DATCBB010000411         1042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_208051, whole genome shotgun sequence.
ACCESSION   DATCBB010000411 DATCBB010000000
VERSION     DATCBB010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208051"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1042)
                     /locus_tag="VII37_04630"
     CDS             complement(<1..>1042)
                     /locus_tag="VII37_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069043.1"
                     /translation="RRFFTISDLVGMRVEDPLVFDATHNLILRLISQGAVVGLRVDHI
                     DGLRDPAGYLKRLQEQLAAEGGSSSASPAYVLVEKILERHEDLPADWPVAGTTGYDYL
                     NFANRVMVYTQGAKEIEKIYSGFIGQNMKFSDVLYQKKKLVMDTILAVEMRSLGRQLE
                     ELAAQARYARDLPRHQLTEALIETTACFQVYRTYIRNLDLPADATKLLEEALAQARLR
                     KPGLNPQCFDFLQDVLLLSNAPHVLPDHREARLNFVLRWQQFTGPIVAKGLEDTALYV
                     YQPLLSLNEVGGDPTPGSVCPKEFFEFIAHRQEKWPHSMNASSTHDTKRSEDVRARIA
                     VLSEMPQEWEARL"
BASE COUNT          230 a          328 c          267 g          217 t
ORIGIN      
        1 tcaggcgggc ctcccactct tgcggcattt cggataaaac agcgatgcgc gcgcgcacat
       61 cctccgagcg cttggtgtcg tgcgtcgagc tggcattcat cgaatgcggc catttttcct
      121 gccggtgggc gatgaattca aaaaactctt tcgggcagac gctcccggga gtcggatcgc
      181 ctcccacctc attcaacgaa agtaacggct ggtaaacgta cagcgcggta tcctccaggc
      241 ccttggccac aatcggtccg gtgaactgct gccagcgcag cacgaaattc aaccgcgcct
      301 cacgatgatc gggcagcacg tgcggagcat tcgaaagcag caacacatcc tgcagaaaat
      361 cgaaacattg cggattcagc ccgggctttc gaaggcgcgc ctgcgccaga gcctcctcca
      421 aaagtttggt agcgtccgcg ggcaaatcga gatttctgat atacgtgcga tacacctgaa
      481 aacacgcggt ggtttcgatc aacgcctcgg ttaattggtg ccgcggcaaa tcacgcgcgt
      541 agcgcgcttg cgccgccaat tcttccagtt gccggcccag agaccgcatc tccaccgcga
      601 gaatcgtatc catcaccagt ttctttttct ggtacagcac atcgctgaac ttcatgttct
      661 ggccgatgaa gccggaataa attttctcga tctctttcgc gccttgcgtg taaaccatca
      721 cccggttcgc aaaattcaga tagtcatatc cagtggtgcc cgccaccggc cagtcggccg
      781 gcagatcttc atggcgctcc agaatttttt ccaccagtac atacgcggga ctcgcagagg
      841 atgagccgcc ttccgccgcc aattgttcct gcagccgttt caaatacccg gccggatcac
      901 ggagcccgtc gatatggtcc acccgcaatc ccaccaccgc gccttgcgag atcagccgca
      961 aaatcaggtt gtgcgtggcg tcaaacacca gaggatcttc tacgcgcata cccaccaggt
     1021 cgctgatggt aaaaaagcgc cg
//
LOCUS       DATCBB010000412         3190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_337835, whole genome shotgun sequence.
ACCESSION   DATCBB010000412 DATCBB010000000
VERSION     DATCBB010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337835"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VII37_04635"
     CDS             complement(<1..445)
                     /locus_tag="VII37_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5069044.1"
                     /translation="MIRTLLVSVFMSLYILLVGPPLLVYTLITRNPDPLFRAGIGGVM
                     FFVRAAGVRVRVVGTERIPATACLFAANHTSTLDAPAIVGAIPRRIAILLKESLFRWP
                     IVGQAFRLAHFIPIERGGRDSAIASLDKATEELRAGQSFLIYPEGT"
     gene            complement(508..2376)
                     /locus_tag="VII37_04640"
     CDS             complement(508..2376)
                     /locus_tag="VII37_04640"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5069045.1"
                     /translation="MIGFSFRAARSLAFSLFLLGFASCPLHGTIRYEVSLAHPEQHLF
                     HVTMTIPDVENEVLVQIPAWNALYQIRDFSAHIQQVEVRRSPTQPGSAGISSDSVPPS
                     IEKIDKQTWRIRCPECHGGHGTITVRYATYWDEPGPFATQLNREHAFINPAMILMYVP
                     ERRSEQVQLVIGDIPDFWNGEGPGIQADERMDRAHAFSSEFPDYDGLADAPIEVGKFE
                     EFQLSGISPEVWVVIHGDNYKKKQIETELKRICEYEIKLMGKAPYSRYTFIVHTGKAA
                     AGAGGGMEHADSTAISVQSAESLPSVAAHEFFHLWNVKRIRPRTLYPIDYTKEQYTRA
                     LWFAEGVTSTYGSYTLERSGLWSKQQLYHDLSEQITELEARPANRWQSAEQSSLDAWL
                     EKYPLYENPEDSVSYYTKGQILGVLLDILIRDRTENAKSLDDVLREMNNDFAMSGKTY
                     RDSLDVQLTAEKVAGGSFEDFFKHYVAGASPLPYQAIFVLAGLELRQTTRQHAALGFA
                     ADGVSKGPLLVHSVEANSAAAEAGLQIGDLILKWNGGDPPRRLADWLRQHKSGESLHL
                     TIRRDDGEVKIDFRLGEITETFYDLAEDSHAGDKARHIREGLLHGTTDAATLHAAN"
     gene            complement(2373..>3190)
                     /locus_tag="VII37_04645"
     CDS             complement(2373..>3190)
                     /locus_tag="VII37_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY5069046.1"
                     /translation="VNLVPRRGRAVIWGDTADSGPALRRAAQKALCQVETYGFSDGND
                     WVASNVAIEKDSVRFAVSHHGERFGEFVLAATGRHNVLNALAAIVVAQGRGISSEAIG
                     KALKTFRSVKRRMDVKGEFGGVLVVDDFAHHPTAVKATIEAARARWPGRRLWGILEPR
                     SNSMRRKVFQETLPQALALADRVVLGGVFRSQQLGDDNRLDPEIVAQSIRSLGKEARV
                     FGSSDEIAGVLAAEAEPGDLLLVMSNGSFDGLCEKLTKKLGEEARVPSGATSR"
BASE COUNT          696 a          937 c          864 g          693 t
ORIGIN      
        1 gcgtaccttc gggataaatt agaaatgatt gcccggcgcg aagctcttcc gtggctttgt
       61 ccaggctggc aatggcagaa tcacggccgc cgcgttcgat cgggatgaag tgcgccaggc
      121 ggaaagcctg ccccacgatg ggccagcgaa aaagcgattc cttcaagagg atggcaatac
      181 ggcgcggaat tgcgccgacg atcgcggggg cgtccagcgt gctggtgtga ttcgcggcaa
      241 aaagacacgc agttgccgga atgcgttctg ttccgacgac tcgcacgcgt acgccagcag
      301 cgcgcacaaa aaacataacg ccgccgatgc ctgctcgaaa aagagggtct ggattgcgtg
      361 tgatcagcgt gtataccagc agcggtgggc cgaccagcag tatgtacagc gacataaaaa
      421 cgcttaccag cagtgtacga atcatggaag gctcagcact gattgcagtg gggtcgcgga
      481 gctgttgcgc ggctgcatgc ggcaggttta attcgctgca tgcagtgttg cggcatccgt
      541 ggtgccatga agcaatcctt cgcggatatg gcgcgccttg tctcccgcgt gagagtcttc
      601 ggcaagatca taaaaggttt ctgtgatttc accgaggcga aaatcaattt tcacttcacc
      661 atcgtcgcgc cggatagtca gatgcaacga ttcgccggat ttatgttggc gaagccagtc
      721 agcaagacgc cgcggcgggt ccccgccatt ccacttcaaa atcaggtcac caatttgcag
      781 tcctgcttcc gcagccgcac tgttggcctc gacgctatga acgagcagcg gccccttgga
      841 tacgccgtcg gcagcgaaac ccagggccgc gtgttggcga gtagtttgcc gcaattccaa
      901 accggccaac acaaagatgg cttggtacgg caatggactc gcgcctgcga cgtaatgttt
      961 aaagaaatcc tcaaacgacc cacccgctac tttctccgcg gtcaattgca cgtccaggct
     1021 gtcgcgataa gtcttgccgg acatggcgaa atcattgttc atttcgcgca ggacgtcatc
     1081 aaggcttttc gcattttccg tgcggtcacg aatcaatata tccagaagta cacccaggat
     1141 ttgccctttc gtgtaatacg aaacactatc ttcgggattt tcgtacagcg gatatttctc
     1201 aagccaggcg tccaggctgg attgttccgc gctttgccag cgattcgcgg ggcgcgcttc
     1261 cagttcggta atctgttcgc tcaggtcgtg gtacagctgc tgcttgctcc aaagacctga
     1321 tcgctccagc gtgtaagatc cgtatgtact ggtgacccct tcggcgaacc acagcgcgcg
     1381 cgtgtattgc tctttcgtgt agtcgatcgg atacaaggtt ctgggtcgga tgcgctttac
     1441 attccacaaa tggaaaaatt cgtgcgcagc gacgctgggt agagactccg ccgactgcac
     1501 agagatcgcg gtggagtcgg catgttccat cccgccgccc gctccggcgg cggccttgcc
     1561 ggtatgcaca ataaatgtgt agcgcgagta cggcgccttg cccatcagct ttatttcgta
     1621 ttcgcaaatg cgcttcagct cggtttctat ttgcttcttt ttgtaattat cgccgtgaat
     1681 caccacccag acttccgggc ttattcccga aagttgaaac tcttcgaatt tccctacttc
     1741 gattggcgcg tcagcaagcc catcgtaatc cgggaactcc gacgaaaaag catgggcccg
     1801 atccattcgt tcatccgctt gaattccggg accttcccca ttccagaaat cagggatgtc
     1861 gccgattaca agttgcacct gctcagaacg gcgctccggc acgtacatca aaatcatcgc
     1921 gggattgatg aaggcgtgct ctcgattcag ttgtgtcgca aagggcccgg gctcgtccca
     1981 gtaagttgcg taccgcacgg tgattgttcc gtggccaccg tggcactcag ggcagcggat
     2041 gcgccaggtt tgcttgtcga ttttttcgat ggacggcggt acagagtcag acgatatgcc
     2101 cgcgctcccc ggttgggtag ggcttcgtcg aacctctact tgctgaatat gcgcactaaa
     2161 atcgcgaatc tgatacagcg cgttccaagc cggaatctga acgaggacct cgttctccac
     2221 atccggaatc gtcatcgtaa cgtggaataa atgctgctcc gggtgcgcga gggaaacttc
     2281 gtaacgaatc gtgccatgga gggggcacga tgcaaaaccg agaagaaaaa gagagaaagc
     2341 tagactgcgt gcagcacgga aagaaaaacc aatcacctgc tggtggcccc ggatggcacg
     2401 cgagcttctt cgcccaattt cttcgttagt ttttcgcaca atccatcaaa gctgccgttg
     2461 gacatcacca gcaacagatc gcccggctca gcctcggccg ctagcacgcc tgcaatttcg
     2521 tcgctgctgc caaaaacgcg ggcctctttc cccagcgagc gaatgctttg cgctacgatc
     2581 tcagggtcga gacggttgtc atcgccgagc tgctgcgaac gaaacacccc gccaagcact
     2641 acgcgatccg ccaacgccag cgcttgcggc agagtctcct gaaaaacttt acgcctcatg
     2701 gaatttgagc gtggctcgag gatgccccac agccggcgcc ctggccaacg tgcgcgtgcc
     2761 gcttcaatcg ttgctttcac cgccgtagga tgatgcgcga aatcgtcgac gaccaaaaca
     2821 ccgccaaatt cgccctttac atccatgcgc cgcttcacgc tgcgaaaagt cttcagtgcc
     2881 ttgccgatgg cttcggagga aattcctctg ccttgtgcaa caacaatggc cgcgagtgcg
     2941 ttcaacacgt tatgccgccc ggtagcggcc agcacaaatt ccccaaagcg ttcgccatga
     3001 tgacttaccg cgaagcgcac gctgtctttt tcaatggcga cattgctcgc aacccaatcg
     3061 ttaccgtcag agaatccgta ggtctcgacc tggcacagag ctttctgcgc cgctctccgc
     3121 aaagccggcc ccgagtcggc ggtatcaccc cagatcaccg cgcggccccg ccgcggcacg
     3181 agattcacca
//
LOCUS       DATCBB010000413         1258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_273398, whole genome shotgun sequence.
ACCESSION   DATCBB010000413 DATCBB010000000
VERSION     DATCBB010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273398"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VII37_04650"
     CDS             <1..540
                     /locus_tag="VII37_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY5069047.1"
                     /translation="VILTCLVSSCSLLARQTVIDIHGSAEELLAQGRGKVLVFVFVRT
                     DCPISNRYAPLLQEMNRKYGQQVLFRLVFPDRTESPDKIRNYLEEYGYQLAAIRDLDH
                     ALVKKTMAKATPEAAVFDAKGQLVYHGRIDNLYEHLGQSRRAASTHELADAIEAVSKG
                     VNPPVATAEAVGCFISDLE"
     gene            801..>1258
                     /locus_tag="VII37_04655"
     CDS             801..>1258
                     /locus_tag="VII37_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069048.1"
                     /translation="MPPWLPEPGEFKFADELRLSDAQIAMIAQWVEQGAVEGAPSDLP
                     PTPSFAEGWQMGTPDLVIKADKPYQLPASGTDQYWNFIFRTPVNKTRWLKGIEVRPGD
                     QRVVHHANVLVDRMESARAQESSPGAGFRGMEIKIESEAFDPDSHFLFWKP"
BASE COUNT          283 a          330 c          347 g          298 t
ORIGIN      
        1 gtcattctga cctgtttggt tagctcctgt tcgcttctgg cccgccaaac tgtgatagac
       61 atccatggct ccgcggagga gctgctcgcg cagggccggg gcaaagtgtt ggtctttgtt
      121 tttgtgcgca cggattgccc aatttcaaat cgctatgcgc cattgctgca agagatgaac
      181 agaaagtatg gacagcaagt cttgtttcgc ctcgtttttc cggacagaac cgaatcgccg
      241 gataaaattc ggaactatct agaggagtac ggctaccaat tggcggcgat tcgcgatcta
      301 gaccacgcac tcgtgaaaaa gaccatggcg aaggcgacgc ccgaggccgc agtctttgat
      361 gctaaaggac agcttgttta tcacgggcgc atcgataatc tctatgagca ccttggacaa
      421 tcacgacgtg cggccagcac gcatgaatta gcggatgcca ttgaagccgt cagcaaaggt
      481 gtcaatcctc cagtggcaac ggcggaagcc gtcggatgtt ttatttcgga ccttgaatga
      541 ttcgggcggg acaaacgatt tctcggctgg ctattttcct gttgtcgtat tcaatgtctg
      601 gcctaacggc gccgcagtct ccgccagaaa cccgaaatac taattcacag gttacgttta
      661 acaaggatat tgcttcgata ttgtttcact actgcgctgc gtgccatcga cccggcgaat
      721 ccggtccctt ctcattgctt tcctataagg atgcgaaatc gcatgcgcgg caaatcgcgg
      781 ccgttacgca aagcaggttc atgccaccgt ggttgcctga gccaggcgaa ttcaaatttg
      841 cggatgaact tcgactctcc gatgcgcaaa ttgccatgat cgctcagtgg gtggaacaag
      901 gcgcggtgga aggcgcgcct tccgacctgc cgcccacacc gagttttgcg gaaggctggc
      961 agatgggcac tccggattta gttatcaagg cggacaagcc gtatcagctc cctgccagtg
     1021 gcactgacca atactggaat tttatttttc gtactccggt gaataaaacc cgctggctga
     1081 aaggcatcga agtacggcca ggcgatcagc gggtggtgca tcacgcaaac gttttggtgg
     1141 atcgcatgga gtcggcgcgc gcgcaggaat cttctcccgg cgcaggcttc cggggcatgg
     1201 aaattaagat tgagtcagaa gcgttcgatc ccgacagcca tttccttttc tggaagcc
//
LOCUS       DATCBB010000414         1017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_78061, whole genome shotgun sequence.
ACCESSION   DATCBB010000414 DATCBB010000000
VERSION     DATCBB010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78061"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VII37_04660"
     CDS             complement(<1..358)
                     /locus_tag="VII37_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY5069049.1"
                     /translation="MSDRIIPRLLRNIVVLSILLLGPAFWFYRLAGAIGFAFGSAVSY
                     VNFRSLSRGVEGLAARIVDRNSREKGGRIILRSVLRYLLVGAVAYAIFKSSELAFRGF
                     LWGLLVPVAALMVEAGW"
     gene            complement(390..626)
                     /locus_tag="VII37_04665"
     CDS             complement(390..626)
                     /locus_tag="VII37_04665"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY5069050.1"
                     /translation="MADPPDYPPRKQPLTSFARYSEIGFIIPACVLLGLLFGKLADYW
                     LHTHWLYVAGIIFGAVVGFWQMIRMAVGAFKKDE"
     gene            complement(675..>1017)
                     /locus_tag="VII37_04670"
     CDS             complement(675..>1017)
                     /locus_tag="VII37_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5069051.1"
                     /translation="FLLSLAGIPPAAGFLGKYYIFLSLIESGHYGLASLGVIYSLLGL
                     YYYLKIANAMLMRAPMEPGRLPVSLGMRCAVGVTALATLVIGVYPEPFIQSVNWSMGI
                     GQSRGARATRR"
BASE COUNT          282 a          319 c          244 g          172 t
ORIGIN      
        1 cccatccagc ttcaaccatc aacgccgcta ccggcactaa caagccccac aaaaagccgc
       61 gaaacgccag ctcagaactc ttgaatatag cataggcaac cgcgcccacc aaaagatagc
      121 gcaacacgga gcgcaggatg atccgcccgc ctttctcgcg gctgttccgg tcaacaatgc
      181 gcgccgcaag cccttccacg ccccgcgaca gggagcgaaa attcacatag gaaaccgccg
      241 acccgaaggc aaacccaatt gcgccagcta gacgataaaa ccaaaatgcc ggccccagca
      301 gcagaatgct caaaaccaca atgttgcgca gcaggcgagg aatgatccga tcagacatcg
      361 acacttcggc gggagaggga tcgccaggtt cattcatctt tcttgaaagc gcccaccgcc
      421 atgcgaatca tttgccaaaa tcccaccacg gcgccaaata taatccccgc cacatacagc
      481 cagtgcgtat gcagccagta gtccgcaagt ttcccgaaca gtaggccgag caagacgcat
      541 gcgggaataa tgaagccaat ctccgaatag cgcgcgaacg acgtaagagg ttgtttcctg
      601 ggcggatagt ccggcgggtc tgccatgcga aggaattgta aatcagcccc gggatactgc
      661 tcggatcatt atcatcatct tcgcgtggct cttgcacctc gcgattgccc gatccccatt
      721 gaccagttca cgctctgaat aaaaggctca ggataaactc cgatgaccag tgtggccaac
      781 gcagtcacgc ccaccgcgca ccgcattcca aggctaaccg gcagtcgtcc tggctccatc
      841 ggcgcgcgca tcaacatcgc atttgcaatt ttcaagtagt aataaaggcc taacaacgaa
      901 tagataacgc cgagcgatgc cagcccgtag tgtccgcttt cgatcaggct taaaaatata
      961 tagtacttcc ccagaaatcc ggccgcgggc ggaatgcccg cgagagagag aagaaac
//
LOCUS       DATCBB010000415         2858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_143692, whole genome shotgun sequence.
ACCESSION   DATCBB010000415 DATCBB010000000
VERSION     DATCBB010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143692"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VII37_04675"
     CDS             complement(<1..683)
                     /locus_tag="VII37_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY5069052.1"
                     /translation="MNKKTKPIVLTVLDGWGYRAETKGNAIALARKPTYDELLRKFPN
                     TLIHTSGPFVGLPEGQMGNSEVGHMNIGAGRIVHMDITRIDLLIANKQLESVPLWQQA
                     MECGRERQLHFIGLLSDGGVHSHIQHLFTLLEMAKHKKVEKVFVHCFMDGRDTPPHSG
                     REFVKQLQHKMRELGVGQIATLVGRYYAMDRDNRWERIELAYRAMVHGEAPTRTDDPV
                     AALGKSYEQ"
     gene            938..1432
                     /locus_tag="VII37_04680"
     CDS             938..1432
                     /locus_tag="VII37_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY5069053.1"
                     /translation="MRAMNRFEQQFRGATTLQEQINHIFGEGVRHTGEESNLTPWAPA
                     VDIYETENELVVKADLPDVNPQNLDIRVENNILTIRGERKFESKVNEDNYLRIERAYG
                     SFSRSFSLANSVKADAIKADYQNGVLTLSLPKREEAKPKQIKVTVGTHAVGTHTMAAA
                     AGAR"
     gene            complement(1712..>2858)
                     /locus_tag="VII37_04685"
     CDS             complement(1712..>2858)
                     /locus_tag="VII37_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069054.1"
                     /translation="KKLSRFYARLQAGDQQMKAFREVFGDLKTVDAGLRQYIQAFAFR
                     AYVVENPNAVQAKDFSSRKLSHSESDAEIAGYRLWEHDGPEATGLVDRALQDDPALGA
                     AHEEKAFIYFREDKDEDALREFSRACELDKTLYLSQYFKAMMTGKRETPEQREALRAE
                     LEQIKQINPQFAPAYVQLAILFIADGQGTKALVQSRKAEQLEPSRAGYHLLSGEMLLR
                     MKREKEAAEAARYVAERWHGPDHNEAVALWNRIPAASRPADAVVVEEVEEQSQAAEGK
                     LRSLSCGEKGKNEVTLQRGDDTMVFKSKGRQMIGYSDTLWYGSDHFSLCHHIEGMHAI
                     VRYRPAVSKDYAGDWLSIELRDELPPEPQQQAAKAPQPQAPAPAKQE"
BASE COUNT          600 a          726 c          844 g          688 t
ORIGIN      
        1 tcctgctcgt aactcttgcc gagggctgcg acgggatcgt cggtgcgcgt gggcgcttcg
       61 ccgtgcacca tggcacggta ggctagttcg atgcgctccc agcgattgtc gcggtccatg
      121 gcgtagtagc ggcccaccag cgtggcgatt tggcccacgc ccagctcgcg catcttgtgc
      181 tgcagctgct tgacgaattc gcggccgctg tgcggcggcg tgtcgcggcc gtccatgaag
      241 caatgcacaa agactttctc tacttttttg tgcttggcca tttcgagcag cgtaaacaaa
      301 tgttggatat gagagtgcac gccgccatcg cttaacaatc cgatgaaatg gagttggcgt
      361 tccctgccgc actccatggc ctgttgccag agcggcacac tctccaattg tttattggcg
      421 atgagcagat cgatgcgggt gatgtccatg tgcacgatgc ggccggcgcc gatattcatg
      481 tggcccactt cactgttgcc catttggcct tcggggaggc ctacgaaagg gccggaagtg
      541 tgaattagag tgttgggaaa tttgcgcagc agctcatcgt aggtgggctt gcgggccagc
      601 gcgatggcat tgcctttggt ttccgcacgg tagccccagc catcgagcac ggttaacacg
      661 attggtttcg tttttttatt catagcgcgt cagaatacac ggattcgcgg cgctcttgta
      721 gtgaaggcgc tgaattgtgg cgtcagttcc cgatgacgtg agtataaaaa ctggaatttt
      781 ggggcacgtg agcattgtga cactggaaca cgttttgcac agaggtgtaa gacgctgccg
      841 ccgcacgact tatatttatt aaataattac aattatatat ctgtacatca gcgatgggtg
      901 cgctcatggt ggaagtgtaa tcaattagga ggctcacatg agagcaatga atcgttttga
      961 acaacagttc cgcggggcca cgaccctaca ggaacagatc aatcacattt ttggcgaagg
     1021 cgtaagacac acgggcgagg agtcgaactt gacgccttgg gctccggctg tggacatcta
     1081 cgagaccgaa aatgaactag tggttaaggc ggacctgccg gatgtgaatc cgcagaatct
     1141 ggatatccgc gtggagaata atattctcac gattcgcggc gaacgcaagt ttgagagcaa
     1201 ggtgaacgag gacaactatc tgcgcatcga acgcgcctat ggctcgttca gccggagctt
     1261 ctcgttggcg aactccgtga aagcggatgc aatcaaggcc gattatcaga atggtgtgtt
     1321 gacactaagc cttcctaaac gcgaggaagc gaagccgaag cagatcaagg tgacggtagg
     1381 aacgcacgct gtaggaacgc acacaatggc agcagcagct ggcgctcgct aagagagtta
     1441 accaaacacg gagcatttgg gcgggcgggt ggcggaaact aaaagccacc cgccttcatt
     1501 tttgaactac acgatgaact cccgcagtgg taactttttc actggcaaag acgcccaaga
     1561 gtccacacga ctaattagag ttaagcggcc gctgtcattt cgttagagtg actttgaatc
     1621 tatgttctcg agacttcgtg gaaggaacgt cacacgaacc gcaacgctgt aattcgtgaa
     1681 gattgcttga tgctatgttc ttctcgaatc cttactcttg tttggccggc gcaggcgctt
     1741 gcggctgcgg tgcctttgcg gcttgctgtt ggggctcggg gggtaattcg tcgcggagtt
     1801 cgatggagag ccagtctccg gcgtaatcct tgctgactgc ggggcggtaa cggacgatgg
     1861 cgtgcatgcc ttcgatgtgg tgacagaggc tgaaatggtc gctgccgtac cagagggtgt
     1921 ctgaatagcc gatcatctgg cggcctttgc ttttgaaaac cattgtgtcg tcgccgcgct
     1981 gcaaggtaac ttcgttttta cctttttcgc cgcaggacaa tgagcgtagt ttgccttcgg
     2041 cggcttgcga ttgttcctcg acttcctcga ccaccacggc gtcagcgggc ctgctggcgg
     2101 ctgggatgcg gttccagaga gctacggctt cgttatgatc ggggccgtgc cagcgctcgg
     2161 cgacatagcg cgcggcctcg gcggcttctt tttcgcgctt catgcggaga agcatttcgc
     2221 cactcaatag atgatagccg gctcgcgaag gttccagctg ctccgctttc cgcgactgca
     2281 ccagggcttt ggtgccttgt ccatcggcga taaataagat cgcaagttgt acgtaggcgg
     2341 gagcaaattg cgggttgatt tgctttatct gctctaattc ggcgcgcaag gcttcgcgct
     2401 gttcgggtgt ttcgcgcttg ccggtcatca tggccttgaa atattgcgaa aggtaaagcg
     2461 ttttatctaa ctcacaggcg cgcgaaaatt cgcgcagggc gtcttcatct ttgtcttcgc
     2521 gaaaataaat aaaggctttc tcttcgtgcg cggcgccgag tgcggggtcg tcctgaagcg
     2581 cgcggtctac caggcccgta gcctccgggc catcgtgttc ccacaaccgg tagccggcaa
     2641 tttccgcatc ggattccgaa tgggacaatt tacgagagga aaaatccttg gcctgcacgg
     2701 cgttggggtt ttcgacgacg taggcgcgga aggcaaaggc ctgaatatat tgtcgcagtc
     2761 cagcgtccac agttttcaga tctccaaata cctcgcggaa ggctttcatc tgctggtcac
     2821 cggcttgtaa gcgggcataa aaacggctca gctttttg
//
LOCUS       DATCBB010000416         2986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_111669, whole genome shotgun sequence.
ACCESSION   DATCBB010000416 DATCBB010000000
VERSION     DATCBB010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111669"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VII37_04690"
     CDS             complement(<1..423)
                     /locus_tag="VII37_04690"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5069055.1"
                     /translation="MPQTENPGSAGVAVFGFQNFRLFQIARFCMVLALEMQSVAVGWQ
                     IYEITHRALDLGYVGLAQFLPGILLFLVSGNVVDKFDRRGILVSCYCGYVLCSTLLLV
                     VALRAGKSVGLIYGILILIGVVRSFNGPVSRAILPQLVP"
     gene            complement(605..1135)
                     /locus_tag="VII37_04695"
     CDS             complement(605..1135)
                     /locus_tag="VII37_04695"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY5069056.1"
                     /translation="MVIGDLKMVILVMGIEGTGKTTVGKLLADRLHWLFADADDFHSA
                     ANKEKMKQGIALTDADRAPWLAGIRAQIWRWIAARINGVITCSALKQSYRDELLSSPP
                     GVDGTPDEIKTVYLHGTYALIAERLHQRQGHFAGEALLASQFATLEEPQDAISIDIDQ
                     TPEQIVNEVMRRLNLA"
     gene            1279..1488
                     /locus_tag="VII37_04700"
     CDS             1279..1488
                     /locus_tag="VII37_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069057.1"
                     /translation="MPNDSHQRAAEFHEQAAHAHRAAAVHHGKEDHLTGHEHSKQAME
                     FSFKAHQASQFAHEKSQNAAQKPGK"
     gene            1724..>2986
                     /locus_tag="VII37_04705"
     CDS             1724..>2986
                     /locus_tag="VII37_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069058.1"
                     /translation="MCWPCRMVYPIFFLPLLASTASAQTSSIESQPASQEQKHGGCLE
                     SPDAPPSMCVPASDKNENDASAETAVKLTAKIDLVVPQGSTLRIALDQRTRISHPGDV
                     VHGKVVETIYAFDQPVIPAGSIATGHVIKVDPVSGVRRTMSYANGDFSPFHKYGVTFD
                     TVTLPDGRQLPVATSVSAGTAQVVHLSTSAEKQKSAAQRAADAGKGKIQEAKDQIHES
                     WEQVTAPGKMDRLKRYIVAQSPYRHQYIEPGTRFNADLDSAISFGEIQRSSDDFTALA
                     SALPPNTTLKARLTGEVSSATATRGTRVEAVLTEPLYSPAHQLILPVNSRIVGQVVQA
                     KAAAKFHHNGELRLIFERIEIPADAQRAAAETAPKLVAQNSANDSRPELTHRGNLRMT
                     GNIEGVDVDRRDHMVLDEEGGARTTDSKT"
BASE COUNT          721 a          880 c          779 g          606 t
ORIGIN      
        1 gggcacaagt tgcggaagaa tggcgcgact gaccgggccg ttaaacgatc gcaccacgcc
       61 gataagaatc aaaatgccat aaatcaatcc gactgatttg ccggcccgca gcgccaccac
      121 cagcaacaaa gtggagcaca gcacgtaacc gcaataacac gacaccagga tgccccgccg
      181 atcgaatttg tcgacaacgt tgccggaaac caggaaaagc aaaatccccg gtaaaaattg
      241 cgcgaggccg acgtagccga gatccagcgc gcgatgggtt atttcgtaaa tctgccagcc
      301 caccgctacc gactgcattt ccagtgcgag gaccatgcag aagcgcgcga tctggaagag
      361 gcgaaaattt tggaagccga ataccgcgac gccagcggac cctgggttct ctgtctgggg
      421 cattaccgtt actttgccac aatcacaaaa atttcgagcg gcttacgccc ttcaacagcc
      481 tccatcagcg aaaatcaccg aacggcggtc ccagttcgca gctgaggtat gagccgtatt
      541 gccttctcgc gcgcgatttg caggtagcga tccgtcgctc atcgcacaga ctcagccagt
      601 tgggttatgc aagattcagg cgtcgcataa cttcgttgac gatctgctct ggtgtctggt
      661 cgatgtcgat gctgattgcg tcctgtggtt cttccagagt tgcgaattgg ctggcaagca
      721 gggcttcgcc ggcaaagtgt ccttggcgct ggtgcagacg ctcggcgatc agcgcgtacg
      781 ttccatgcag gtataccgtt ttaatttcat ccggagttcc atccacgccg gggggcgacg
      841 acaacaattc atcgcgatag ctttgtttca gcgcggagca ggtgatcacg ccatttattc
      901 tggcggcgat ccagcgccag atttgcgccc ggatgcccgc gagccacggc gcgcgatccg
      961 catcggtgag cgcaattccc tgtttcattt tttctttatt ggcggcggaa tggaaatcgt
     1021 cagcgtcggc gaacaaccag tgcagccgat cggccaggag ttttcccacg gtggttttcc
     1081 ccgtgccttc aatacccatc accagaatca ccatttttaa gtcaccgata accacgggct
     1141 aaaaatcctg acacgttgtt cggggctgcg cacgcatgag ttcacgaatc ggaccgcgta
     1201 caatacagca cagccgcgcc gcactgtgag cgcctaaaat tatgagcaca tcgcaagctt
     1261 gtgttaaaaa ggattatcat gccaaacgat tcgcatcaac gcgccgctga atttcacgaa
     1321 caggccgcgc acgcacatcg cgccgccgca gtgcatcacg gaaaagaaga tcatctcacc
     1381 gggcacgaac actccaagca ggccatggag ttttctttca aagcgcatca ggcttcgcag
     1441 ttcgcgcacg agaaatcgca aaatgccgcg caaaagccgg gcaagtaact tcacggtcaa
     1501 acgcctctac gcttgaggcc cggcatcaaa gccccgcttt taattgccga aaatttctgt
     1561 cgcccgcggc aacttttcgc ctggcagagc gcatccatgg cttcagcgtg cgcgcaggca
     1621 cgcaggtgga caaagccatc gtggcgcgtt atgttgttgt ttcagtaagt acgatactcg
     1681 taatcgcatt ttctcccccc gaagatgctt taaggaaaca ttgatgtgct ggccctgtcg
     1741 tatggtgtat ccaattttct ttttgccgtt gctggcatcg accgcatcgg cgcaaaccag
     1801 ttccatcgaa tcgcaacccg cttctcaaga acaaaaacac ggcggttgcc ttgaatcgcc
     1861 cgacgcgccg ccttccatgt gtgtaccggc gtcggataaa aatgaaaatg acgccagtgc
     1921 ggagacggcc gtaaaactca ccgcgaaaat tgatctggtt gtgccacaag ggtcgacact
     1981 gcgcattgca ttagatcagc gcactcgcat ctcccatccc ggcgacgtcg tgcacggcaa
     2041 agtcgtcgaa accatttacg ctttcgatca acccgtcatc cccgcaggca gcattgctac
     2101 aggtcacgtc attaaagttg atcccgtgag cggcgtacga cgcactatgt cctatgccaa
     2161 cggcgacttt tccccatttc acaaatatgg cgtgacgttt gataccgtaa ctctgccgga
     2221 cggccggcaa cttccggtgg ccacttccgt ttcggccggc acggcacagg tcgtgcacct
     2281 gagcacctcc gcggaaaaac agaaaagcgc tgcgcaacgc gctgctgacg ctgggaaagg
     2341 taaaattcaa gaagccaaag atcaaattca tgaatcttgg gaacaagtga ccgcgccggg
     2401 caaaatggac cgcttgaagc gttacattgt ggcgcagtcg ccgtaccgcc accagtacat
     2461 cgagccaggc acacgattta atgccgacct ggacagcgct atttcgttcg gggaaatcca
     2521 gcgaagctcc gacgatttca cagctctggc aagcgcttta cctcccaaca ccacattgaa
     2581 ggcacgccta accggcgaag tcagttccgc tacagcgaca cgtggaactc gtgttgaggc
     2641 cgttttgacc gaaccgctgt attcgcccgc acaccaatta attttgccgg taaacagtcg
     2701 aatcgtcggc caggttgtac aagccaaggc cgcggccaag tttcatcaca acggtgagct
     2761 gcggctgatc ttcgagcgca ttgaaattcc tgccgacgca caacgcgcgg ctgccgagac
     2821 ggctccaaaa ctcgtggcgc aaaatagcgc caacgattcc cgaccggaac taacgcaccg
     2881 tggaaatttg cgcatgactg gaaatatcga gggcgtagac gttgatcgcc gcgaccacat
     2941 ggttctcgac gaggaaggcg gcgcacgcac caccgacagc aagacg
//
LOCUS       DATCBB010000417         1854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_273485, whole genome shotgun sequence.
ACCESSION   DATCBB010000417 DATCBB010000000
VERSION     DATCBB010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273485"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="bamD"
                     /locus_tag="VII37_04710"
     CDS             complement(<1..1155)
                     /gene="bamD"
                     /locus_tag="VII37_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY5069059.1"
                     /translation="MLLMAIFLVGANQSGAQILHKKKKVDKSTSVDNNVQPDKVLYDR
                     AADDIKHGKHEVGRLNLQTLINTYPDSEYLAKAKLLIADSYYKEGGTANMTQAISGYK
                     DFIVFFPFLPEASYAQLQVAMGHFKQMEKPDRDRTEAKEAESEFQTFLQKYPKDPLAD
                     KAMQHLREVQEVIAEGDYRVAYYYYAKGDKRAAAARLIGVTNRYPLYSRSDQALWILG
                     DIFDKAEKKEVAANYYARIVKNYPLSKEAPDAKARLVAMKVPVPQPDPKARARMVAEQ
                     NAPRPHTSFAKKPLQVLHSGPNGEMRTAARVGEPNLEPEADTSSATDVLTGGNSSRIV
                     VGGSGGGGATPGTAAGNGIVATVTPGGAKTSEESGATEGAAPADTNAPAAT"
     gene            1437..>1854
                     /locus_tag="VII37_04715"
     CDS             1437..>1854
                     /locus_tag="VII37_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5069060.1"
                     /translation="MKTLLLLASFFIPAAALAQNTTSPGDSGTAMFRGNPQHTGVYDS
                     TGVPAFHKIKWKFHTPGFVISSPAIDVSTAYVGSTDGNLYAVNLATGDQRWKFATEAR
                     ITSSPAIQRGTAYFSSYDGKFYAVDVSTGKLKWKFQM"
BASE COUNT          383 a          587 c          426 g          458 t
ORIGIN      
        1 agtggccgcc ggcgcattcg tatcagctgg cgcagcacct tcggtagcgc cgctctcttc
       61 gcttgtcttg gcgcctcctg gcgttactgt agccacgatc ccattcccag cagcagtgcc
      121 cggtgtcgct ccaccgcctc cgcttccacc tacaacaatg cgcgacgagt tcccaccggt
      181 gagcacatcc gttgccgacg acgtgtccgc ctcaggctcc agattcggct ctcccacccg
      241 agcagcggtc ctcatttcgc cattcggccc ggaatgcagc acttgcagcg gcttctttgc
      301 aaagctggta tgcggccgcg gagcattctg ttccgcaacc atccgcgcac gcgccttcgg
      361 atcgggctgc ggtaccggca ccttcatggc caccaaccgt gccttcgcat ccggcgcttc
      421 tttcgaaagc ggataattct tcacaatgcg cgcgtaataa tttgctgcga cttctttctt
      481 ctccgcttta tcgaaaatgt cccccaatat ccaaagcgct tgatccgatc ggctatacag
      541 cggataacga ttcgtcactc caatcagccg cgctgccgcc gcgcgcttgt cacctttcgc
      601 gtagtaataa taggccactc ggtaatcacc ctcggcgatc acctcttgta cttcgcgcaa
      661 atgttgcatc gctttatccg ccagcggatc tttcggatat ttctgcagga aggtctgaaa
      721 ttcgctttcc gcttctttcg cctcggtgcg atcccggtcc ggtttctcca tctgtttgaa
      781 gtgacccatg gccacctgca attgcgcgta ggaggcctcc ggtaggaaag ggaagaacac
      841 gatgaaatct ttgtagccgg aaatcgcctg cgtcatattg gccgtgccgc cctctttata
      901 ataagagtcg gcaatcaaca atttggcctt ggccagatat tcgctgtcag gataagtatt
      961 gatcagcgtc tgcaggttta accgtcccac ctcatgcttg ccgtgcttga tgtcgtccgc
     1021 cgcgcggtca tataaaactt tatccggttg gacgttgtta tccacgctcg tggacttatc
     1081 gacctttttc tttttgtgta aaatctgggc gccggattgg ttggctccca cgaggaaaat
     1141 cgccatcaac aacaccaacg ctaacctatg gctagttgca tacgacggag tacgtagcaa
     1201 acaacacctc tctaaaaata gtgaaattcc ttcgaatgcc tgccgcggcc gctaggggag
     1261 agccgcccga aaccccgaac tcccggatta taacctgaac gaaaggccag tcttcaccca
     1321 tctttgccct ttaggacagt aaatcccgtt aatgagaacc atccccgcac ccccaggttg
     1381 cctccgtgtc ttctttattt cttaaccttt ctccagcgtc tccgtctaac gcgctcatga
     1441 aaacgctcct gctgctcgcc tccttcttca tacccgccgc cgcgctggct caaaacacga
     1501 catccccggg ggactctgga acggccatgt ttcgcggcaa tccgcagcac accggcgttt
     1561 atgattcaac cggggtgcct gctttccaca aaataaaatg gaaatttcat acccctggct
     1621 tcgtcatttc ctcgcctgcc atcgatgttt ccaccgccta cgtaggcagc accgacggca
     1681 acctatatgc cgtaaatctg gcgactggcg atcaaagatg gaagtttgcc accgaagctc
     1741 gcattacttc ttccccagcc atccaacgcg gcaccgcata tttctcgagc tacgacggaa
     1801 agttctatgc tgtcgatgtt tccactggca aattgaaatg gaaattccaa atgg
//
LOCUS       DATCBB010000418         1565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_46710, whole genome shotgun sequence.
ACCESSION   DATCBB010000418 DATCBB010000000
VERSION     DATCBB010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46710"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VII37_04720"
     CDS             <1..781
                     /locus_tag="VII37_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEY5069061.1"
                     /translation="FVIDLDKADGEMLSYKAGIPTGKKCEKCGEGELLERISRHGFFL
                     GCSRYPDCEFIEDLSPEVPPGDENKIEYCDNCGKEMAIKRGRFGVFLACTGYPDCKTT
                     RRLVEGTRIARQPDEPLDEKCTLCDSGLVKKHGRFGEFIGCSNYPKCKYTRPITMGIK
                     CPKCNEGEFVRRGSAGKGGRGRPRVFYGCSRYPDCDFTTPHMPIAEPCPKCGAPFIVE
                     KRSKIGTVRTCLKEGCDFEVLAPEAQAPVTAEEAVPEPAKV"
     gene            879..>1565
                     /locus_tag="VII37_04725"
     CDS             879..>1565
                     /locus_tag="VII37_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY5069062.1"
                     /translation="MKQAIESYLEYLVSVKNSSPHTISNYGKDLRQFVAFLTPPGMSL
                     PALTAINHGIIREFVAHLHEQGLEKTSIARKLAALRSFFKYCVREGKLNESPARLVPT
                     PKLPKRIPSILSAEEMNSFLNQLEEMGGSLPKTTKKSKKEWPPAEALALPSELKLRAE
                     GLLLRRDRALLELLYAAGLRVSELTGLDLVDIEQKERVLRVTGKGNKERIVPYGNKAQ
                     QALDKYWPLRE"
BASE COUNT          383 a          413 c          423 g          346 t
ORIGIN      
        1 gtttgtaatc gatctggata aagccgacgg cgagatgctt tcctacaaag ccggcattcc
       61 caccgggaaa aagtgcgaaa agtgcggcga gggcgaattg ctggagcgca tcagccgtca
      121 tggatttttc ctgggctgct cgcgttatcc agactgcgag tttattgagg acctatcgcc
      181 ggaagttcct ccgggcgacg aaaacaaaat tgaatactgc gataactgcg gcaaggaaat
      241 ggcgatcaag cgcggacgtt ttggcgtgtt tctggcctgc actggctacc ccgattgtaa
      301 aactacgcga cgcctcgtgg agggcacgcg aatcgcgcgg cagccagatg agccgctgga
      361 cgaaaagtgc acgctgtgcg attccggttt ggtaaaaaaa cacggccgtt tcggcgaatt
      421 tattggctgc tcgaattatc cgaaatgcaa atacacgcgt ccgatcacca tgggcattaa
      481 atgccccaag tgcaacgaag gagaattcgt tcgccgcggg agcgccggaa aaggtggacg
      541 cggccgtccg cgcgttttct acggttgttc gcgttacccc gattgtgatt tcaccactcc
      601 gcacatgccc atcgcagagc cttgccccaa gtgcggcgct ccattcatcg tggagaagcg
      661 cagcaagatc ggcacagtac gtacctgcct gaaagagggc tgcgactttg aggttctcgc
      721 tccggaagct caggcgccgg taactgcgga agaggccgtc ccggagccgg caaaagtctg
      781 aatctctcct tcagttccaa tctaggcata catcgttccg cactgctata cacatctaaa
      841 tgtttaggta tgattttgcg tggggttcag cccgggccat gaaacaagcc atcgaaagtt
      901 atctcgaata tcttgtttcg gttaaaaatt cttcaccgca taccatttcc aattacggta
      961 aagatttgcg ccagtttgtg gcgttcctga ccccgccggg aatgtcactt ccggccctca
     1021 ctgcaataaa ccacgggatc attcgcgaat ttgtagcgca cttacacgaa caaggcctgg
     1081 aaaaaacttc cattgcgcgc aaacttgcgg ctttgcgttc cttttttaaa tactgtgtgc
     1141 gcgaaggaaa gttaaacgag agtcccgcgc gcctggtgcc cacgccaaaa ttaccgaagc
     1201 gcatcccttc gattctttcc gcggaagaga tgaatagttt tttaaatcag cttgaggaaa
     1261 tgggtggatc actgcccaag actacgaaga aatccaaaaa ggaatggcca cctgcagagg
     1321 ctctggcatt gccaagcgaa ctgaagctcc gtgcagaggg cttattgctg cgtcgcgatc
     1381 gcgctttgct ggaacttctc tacgcagccg gtctgcgcgt tagcgaattg acgggcctgg
     1441 acctcgtcga tattgaacag aaggagcggg tattgcgcgt aaccggcaaa ggcaacaaag
     1501 aacgcattgt gccgtacggc aataaggccc agcaagcgct tgataaatac tggccactcc
     1561 gcgaa
//
LOCUS       DATCBB010000419         1126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_403553, whole genome shotgun sequence.
ACCESSION   DATCBB010000419 DATCBB010000000
VERSION     DATCBB010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403553"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VII37_04730"
     CDS             <1..1109
                     /locus_tag="VII37_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lon-insertion domain-containing protein"
                     /protein_id="HEY5069063.1"
                     /translation="LYEVLYQYEEDFRKIFKVRVEFDEEMHMSDGVIAEYAGRLRALC
                     EKENLFPFDRGAFAAMLEYGVRQAGRRNKVTARFVDMADLAREAHYAAMAAGETVVRA
                     AHVRSALASKVERHNLIETRVREMIEEGTLLVDVAGERVGQVNGLSVLEIGGYSFGKP
                     VRITASAALGKAGLINIEREANLSGRFHDKGVHIISGFLRTRFAQDSPLSLAASICFE
                     QSYSGVDGDSASSTEVYALLSALSGLPLRQDIAVTGSMNQQGDIQAIGGVNEKIEGYL
                     GVCRIQGLTGTQGVMIPASNVEDLMLREDILDAVAAGQFHIWPVGKVERGIEILTGRA
                     AGTRDDKGKFEDGTVFALVDERLRAMAITMKDYL"
BASE COUNT          248 a          296 c          344 g          238 t
ORIGIN      
        1 agttgtacga agtgctgtat cagtacgaag aagattttcg caaaattttt aaagtgcgcg
       61 tggagttcga cgaagaaatg catatgagcg atggcgtcat cgccgaatac gccggccgcc
      121 tgcgcgccct gtgcgaaaaa gaaaatttat ttccgtttga tcgcggcgcc ttcgcggcca
      181 tgctcgaata cggagtgcgc caggccggcc gccgcaataa agttaccgcg cgatttgtcg
      241 atatggcgga cctggcgcgc gaagcgcact acgcggcgat ggccgcgggc gaaacggtag
      301 tccgcgcggc ccacgtacga agcgctctgg cttcaaaagt tgagcggcat aatctcatcg
      361 agacgcgcgt acgcgaaatg atcgaggaag gtaccttgct ggtcgacgtg gccggcgaac
      421 gcgtagggca agtgaatggc ctgagtgtgt tggaaattgg cgggtattcc tttggcaaac
      481 ccgtgcgcat caccgcctcc gcagccttgg gaaaagccgg gctcatcaat attgagcgag
      541 aagcgaacct cagcggccgc tttcacgaca aaggtgtaca cattatttcc gggtttctgc
      601 gtacccgctt tgcccaagac tcgccgcttt cgctggccgc cagtatttgt ttcgaacagt
      661 cctactccgg cgtggatggc gacagtgcca gctccaccga agtgtacgcg ttgctctcgg
      721 cgctttccgg cttaccgctg cggcaagata tcgccgtgac cggctccatg aatcagcagg
      781 gtgacattca agccattggc ggagtgaacg aaaaaattga aggatatttg ggtgtgtgcc
      841 gcattcaggg tctgactggc acccagggcg tgatgattcc cgcatccaac gttgaggatc
      901 tgatgctccg cgaagatatt ctcgatgccg tagccgccgg tcaattccac atttggccgg
      961 ttgggaaagt cgagcgggga atagaaattt tgaccggcag ggccgcgggc acgcgggacg
     1021 acaaaggtaa attcgaggac ggcacggttt ttgccctggt ggacgaaaga ctgcgcgcca
     1081 tggcaatcac catgaaggat tacctgtaac ttttcagcgt gtcggc
//
LOCUS       DATCBB010000420         1450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_468128, whole genome shotgun sequence.
ACCESSION   DATCBB010000420 DATCBB010000000
VERSION     DATCBB010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468128"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VII37_04735"
     CDS             complement(<1..313)
                     /locus_tag="VII37_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069064.1"
                     /translation="MNSPIKLIALSIASVAFALSLAVATYGQQRPLVTEDVDIIPPGS
                     MRIEAGIDFVQRAKFPVSGLTGDLTRAGVIGINIGFAPNVEFQIEGVAQNFLSINSRG
                     TS"
     gene            466..>1450
                     /locus_tag="VII37_04740"
     CDS             466..>1450
                     /locus_tag="VII37_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY5069065.1"
                     /translation="MNIKSGKIRLAIMTAVVCAAGYSFHLPAAVAQQANRTVNMPGLH
                     SRVTVRRDERGIPYIEAANDEDLYFAQGYVTASDRLWQMDLMRRTARGELAEILGAGP
                     NNAALEQDKQHRTLGFAQEVNAELAQASPRTRALLEAYARGVNAYIDSLDSKSMPPEF
                     QILQYKPRPWTAADCLLPVKLFAESLSTTWRLDVMREALATVPAEKRAALLPEISPLD
                     VLVVGKDTKKLNPAFANQNGGPGENRIPAPVSTELVAALAKGQEVASQALERIGLYAE
                     GLAASNNWVVSGKRTVSGKPLLANDPHLAASAPSIWHLVHLSAPGLRVAGVT"
BASE COUNT          326 a          432 c          402 g          290 t
ORIGIN      
        1 cagacgttcc gcgggagttg atgctcagaa agttctgcgc tactccttcg atttggaatt
       61 ctacgttcgg cgcgaacccg atattgatgc caatcactcc ggcgcgtgtc agatcgccgg
      121 tcagtccgga aaccgggaac ttcgcccgct ggacaaaatc aattcccgct tcgatccgca
      181 tcgaaccagg tgggatgatg tccacgtcct cggtaaccag cggccgttgc tggccatagg
      241 tcgccacagc aagactgagg gcaaacgcca ccgaggcgat gctgagcgca attaatttga
      301 ttgggctatt cacgttgatt cggttttctc aaacgctctt gaattcacgc ggattgtaat
      361 gcgcgcagga gcaagccgtc aacatcaaag gatcttttta cgccccacga ttgtgctaac
      421 atgctgacct ttgcgagctg gattttccaa aaaaaaggcg aaccgatgaa cattaagtcc
      481 ggaaaaatac ggttagcgat tatgaccgcc gtcgtgtgcg ctgcgggata cagttttcac
      541 cttcctgccg cggttgccca acaagctaat cgcaccgtca acatgccggg cctgcatagt
      601 cgggtcaccg tacgacgcga tgaacgcggc attccctaca tcgaagcggc gaacgacgag
      661 gacctctatt tcgcgcaggg ctacgtcact gccagcgatc gactgtggca gatggatctc
      721 atgcgacgaa ccgcccgcgg cgaattggca gagatcctgg gagccggtcc aaacaacgcc
      781 gcgctcgaac aagacaaaca gcatcgcacg ttgggatttg cgcaggaagt taacgccgag
      841 ttggcccagg cctcaccgcg gacacgcgcc ctgcttgagg cgtatgcgcg cggggtgaat
      901 gcttacatcg actcactcga ttccaaatcg atgccgcccg aatttcagat acttcaatac
      961 aagccacggc cctggaccgc agcggattgc ctgctgcccg tcaaattgtt tgccgaatcg
     1021 ctctctacca cctggcggct ggacgtgatg cgcgaagcgc tggccaccgt cccggcggaa
     1081 aaacgcgccg ccctactacc cgagatatct ccgctcgatg ttctggtcgt aggcaaagac
     1141 acaaagaaac tcaacccagc gttcgctaac cagaatggag gtcctggcga aaacagaatt
     1201 cctgcgcccg tctctacgga attggtcgcg gcgctggcga aaggtcaaga ggtcgcgtcg
     1261 caggcccttg aaaggatcgg tctctatgcc gaaggtctcg ccgccagcaa caactgggtg
     1321 gtgagcggaa aacgcacggt gtccggcaag cctttgctgg caaacgatcc tcatctggcc
     1381 gcgtcggccc cgtcaatctg gcatctcgtt catctcagcg cacccggact gcgagtggcc
     1441 ggggttactg
//
LOCUS       DATCBB010000421         1832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_338146, whole genome shotgun sequence.
ACCESSION   DATCBB010000421 DATCBB010000000
VERSION     DATCBB010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338146"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VII37_04745"
     CDS             complement(<1..498)
                     /locus_tag="VII37_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5069066.1"
                     /translation="MSAAPERLPVAVVGVGEFGRKHARVYRELQNTELLGIFDRDAAK
                     ARAVAEEFHTRVFGNLDELRGCVKAASVAVPTESHSEVGCRLLDMGIDVLVEKPMATD
                     LRQADALLAAAKKNQRILQVGHVERFNPAVIAVEPIVNRPLFFEVHRLGVFTARSLDV
                     DVIYDL"
     gene            complement(495..1379)
                     /gene="lpxI"
                     /locus_tag="VII37_04750"
     CDS             complement(495..1379)
                     /gene="lpxI"
                     /locus_tag="VII37_04750"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEY5069067.1"
                     /translation="MSVNAKTEQWGLIAGNGRFPFLVLEGARSQGLEMAVIALKEEAS
                     EELGKFTSRIHWVSLGELSKAIDLMHQEGVTRAVMAGQVKHNKIFSSIRPDWKLAKLL
                     LSLPRKNTDTLIGAVARVLGDEGIQLVDSTLFLKPLVPDVGVLTRRAPNEREAADIAY
                     GFSVARNIAGMDIGQTVVISDCACVAVEAMEGTDEAVARAARIVGGKPLVVVKVSKPG
                     QDMRFDVPVIGLPSIEQMASAGVTALAVDAGRTLLFDREKLIALAEEKGIAIEAFSPP
                     FSAPGAEPKAGNKGMSQG"
     gene            complement(1520..>1832)
                     /locus_tag="VII37_04755"
     CDS             complement(1520..>1832)
                     /locus_tag="VII37_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069068.1"
                     /translation="QFCRVGRYAYVGALTVITQDVPPFSRVVTERSTKSYGVNSIGLE
                     RKGFSKERLQALGRAYRLLLKSKMNTSQALAEMRATLGDSEDVQELVKFIEAAERGIV
                     K"
BASE COUNT          417 a          641 c          374 g          400 t
ORIGIN      
        1 caaatcgtaa atcacatcca catccaggct gcgcgctgta aatactccca agcggtgcac
       61 ttcaaaaaat aacggccggt tcacgatggg ttctacggcg atcaccgcag gattaaagcg
      121 ctccacatgc ccgacctgca aaatccgctg attctttttc gccgctgcca acagcgcatc
      181 tgcctgcctc agatcggtgg ccatgggctt ctcgacgagt acatcgatgc ccatgtccaa
      241 caaccggcaa ccaacttcgc tgtgcgactc cgtgggaacc gcgacgctgg cggccttcac
      301 gcatccgcgc aattcatcca ggttgccaaa tacgcgagtg tgaaactcct cggctactgc
      361 acgcgctttt gccgcatctc gatcgaaaat gcccagcagt tctgtattct gaagctcgcg
      421 ataaacccgc gcgtgtttgc gcccaaattc gcctacgccc accacggcaa ccggcaaacg
      481 ctccggcgct gcgctcatcc ctgactcatt cccttgtttc cggcttttgg ttccgcgccc
      541 ggtgcagaaa aaggtggaga aaaagcctca atggcaatcc ctttttcttc tgccagagcg
      601 attaatttct cgcggtcaaa caataaagtc ctcccggcat ccacagctaa cgccgtaacg
      661 cctgcgctgg ccatctgttc gatggacggc aacccgatca caggtacgtc gaagcgcatg
      721 tcttgcccgg gcttactcac cttcactacc accagcggct ttcctccaac aattcgcgca
      781 gctcgcgcca ccgcttcgtc cgtgccctcc atcgcctcta ccgccacgca agcacaatca
      841 gaaatcacca ccgtctgtcc aatatccatc cccgcaatat ttctcgccac gctaaatcca
      901 taagcaatgt ccgcggcctc gcgttcattc ggagctcgcc gcgtcaacac ccccacgtcc
      961 ggcaccagcg gcttcaaaaa taaagtcgaa tccaccaatt ggatcccttc atcgccgagc
     1021 acccgcgcca ccgcgccaat caacgtgtcg gtattttttc gcggcaacga cagcaaaagt
     1081 tttgccagct tccagtccgg ccgtatcgag ctgaaaattt tattgtgttt gacctgcccc
     1141 gccatcaccg cgcgtgtcac gccttcctgg tgcatcaaat caatggcctt gctcaattcg
     1201 cccaggctca cccaatgaat tcgcgaagta aattttccca actcctccga tgcttcttct
     1261 ttcagcgcga tcaccgccat ctccagcccc tggcttcgcg ctccctccaa caccaaaaac
     1321 ggaaatctcc cattccccgc aatcaatccc cactgttccg tcttcgcgtt aacactcaat
     1381 ttttagccct acctcagtgt aaataacgcg aagggtgccc caccctcgga tttttggagg
     1441 gtgggtcttg tagcgtggaa tcaccaaaaa gtcagccgcg aagcatttct tcaattttct
     1501 tcccaacaaa cccacactcc tacttcacaa ttccgcgctc cgccgcttcg ataaatttca
     1561 ccaactcctg aacatcctcg gagtccccca gagtcgcgcg catctccgcc agcgcctgcg
     1621 aagtattcat cttcgatttc aaaagcaacc ggtaagcgcg acccaaggcc tgcaaccgtt
     1681 ccttagaaaa tcctttgcgc tccaatccaa tcgaattgac gccgtaactc ttggtgctcc
     1741 gctccgtcac cacccgcgaa aacggaggca catcctgcgt aatcaccgtc aaagccccca
     1801 catacgcata gcgcccaacg cggcaaaact gg
//
LOCUS       DATCBB010000422         3144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_111872, whole genome shotgun sequence.
ACCESSION   DATCBB010000422 DATCBB010000000
VERSION     DATCBB010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111872"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VII37_04760"
     CDS             complement(<1..248)
                     /locus_tag="VII37_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069069.1"
                     /translation="MTRALVLALASVSFLIVAGLALSAVSVSEKARKLHFSSIVVDTH
                     DDTTQRFLDGKFDLGERTNTGSIDIPRMKEGGLSAIFFS"
     gene            complement(365..1126)
                     /gene="sdhB"
                     /locus_tag="VII37_04765"
     CDS             complement(365..1126)
                     /gene="sdhB"
                     /locus_tag="VII37_04765"
                     /inference="COORDINATES: protein motif:HMM:NF006391.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY5069070.1"
                     /translation="MPNKNDKKEVLVKVKRQSSPKESPHWEEFRLQWRPYMNVIICLR
                     DISEHPVTSDGRPTTPVSYDSNCLEEVCGSCAMLINGKARMACSALVDQLEQPIRIEP
                     MTKFPVVRDLSVDRQFMFESLKRVKAWIPIDGTYDLGEGPRVAPEIQEKGYPLSRCIT
                     CGNCLEVCPQVNEHNPFMGAAIFSQVRLFNMHPTGKMHAKERLAAAMGNGGIEDCANA
                     QNCVVACPKGIPLTESLAEINREAWKQSLFGWLAG"
     gene            complement(1167..2951)
                     /gene="sdhA"
                     /locus_tag="VII37_04770"
     CDS             complement(1167..2951)
                     /gene="sdhA"
                     /locus_tag="VII37_04770"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY5069071.1"
                     /translation="MKQPKIIVVGGGLAGLMATIRVAEAGVPVDLFSIVPVKRSHSVC
                     AQGGINAAKNQKGEGDSTAKHFDDTIYGGDFLANQPLVKRMCEAAPGIIDLLDRMGVM
                     FNRTPEGLLDYRRFGGTLYHRTAFAGATTGQQLLYALDEQVRRHESEGKVKKYEGATF
                     LSAVIDNSGVGRGICAMDLRSMEVTTHAADAVIFATGGIGAIFGRSTNSVVCTGSAQS
                     ALFQQGVDYANGEFIQVHPTAIPGEDKLRLMSESARGEGGRVWVPKNPNDQRAPRTIP
                     ERERFYFLEEWYPKYGNLVPRDIATRAIHRIVYQERLGLHGKAAVYLDVTHIPRETLE
                     RKLEGILEIYEKFLGVDPHVEPMQVFPAMHYTMGGIWINGQGQATSVPGIYAAGECEY
                     QYHGANRLGANSLVSCIFGGGLAGTAAVKYSANLHTGTEAVSSTIFENEKKRQEEKNQ
                     TLISNQGSENPITIWRELGEIMTEHVTVTRINKNLELADSKIRDLQERYHKINLSDRT
                     KWSNQTLNFARELENMLILARVVTLGALQRNESRGAHYKPDFPERDDANWLKTTRARF
                     SDGEIQLTYEPVDISQIPPRPRRYDAAK"
BASE COUNT          717 a          813 c          888 g          726 t
ORIGIN      
        1 gagaagaaaa tcgcgcttaa gccgccttcc ttcatgcgcg gaatatcaat gctgccggta
       61 ttggttcgct caccgagatc gaatttgcca tccagaaagc gttgcgtagt gtcgtcgtgg
      121 gtgtccacaa cgatggaaga aaaatgcagt ttgcgggctt tctctgaaac agagacggcc
      181 gagagagcca aacctgcaac gatcaagaaa gagacggaag cgagagccag taccagagcg
      241 cgcgtcattt gttctccaaa ggtttgagtg aaggcagatt gtaaacggaa atggaacgct
      301 atggatggaa aaatggacgt aatgcctcgt tagagcacca gcacggagca aaacggatca
      361 gaagttatcc ggcgagccag ccgaataggg attgcttcca ggcttcgcga tttatttccg
      421 ccagcgattc cgttagcggg atgcctttgg ggcaagccac aacgcaattc tgcgcgttcg
      481 cgcaatcttc gatgccgccg ttgcccatgg ctgcggcaag gcgttctttg gcgtgcattt
      541 tcccggtggg atgcatgttg aaaagccgca cctggctgaa aatcgcggca cccatgaacg
      601 gattgtgctc gttcacttga ggacagactt ccagacaatt gccgcaggtg atgcaacgcg
      661 aaagtggata gcctttttcc tgaatttcgg gggccacgcg cgggccttct ccgagatcgt
      721 aagtaccgtc gatgggaatc caggctttga cgcgcttcaa actctcaaac atgaactggc
      781 gatcgacgga tagatcgcgc accacgggaa atttcgtcat gggctcaatg cgaatgggct
      841 gctccagttg atcgaccagc gcggagcagg ccatgcgcgc cttgccgtta atcagcatgg
      901 cgcaggagcc gcatacttct tcgaggcaat tcgaatcgta ggagactggg gtggttggcc
      961 ggccatcact ggtcaccgga tgctcactga tatcgcgaag acaaatgatg acattcatgt
     1021 acggccgcca ttgaaggcgg aattcttccc agtgcggaga ttctttcggg gaagactgtc
     1081 gctttacttt gacgaggact tcttttttgt cgtttttatt gggcattgga accttctgct
     1141 gcaccacaaa agtttctgat cgcctattat ttggcggcgt cgtaacgcct cggccgtggc
     1201 ggaatctgtg aaatgtcgac aggctcgtag gtcaactgga tttcgccgtc gctaaatctg
     1261 gcacgcgtgg ttttgagcca gttggcgtca tcgcgttcgg gaaaatccgg cttgtagtga
     1321 gcgccacgcg actcgttgcg ctggagagcg cctagcgtaa ccacgcgcgc caagatcaac
     1381 atgttttcaa gctcacgcgc gaaattcagt gtctgattgg accattttgt gcggtcgctc
     1441 agattaattt tatgatagcg ctcttgcaag tctcggattt tggaatccgc caattccagg
     1501 tttttattta tgcgcgtcac ggtgacgtgc tcggtcatga tttcaccgag ttcacgccat
     1561 atggtgatgg gattttcgga tccctgattg gagatcaggg tctgattttt ctcttcctgg
     1621 cgttttttct cattttcaaa aatggtggac gacacggcct cggttccggt atgcagattt
     1681 gcggaatatt tcacggcggc cgttccagcg aggccgccgc caaaaataca agagaccagc
     1741 gaattggcgc cgaggcgatt tgcgccgtga tactgatatt cgcattcgcc ggccgcgtaa
     1801 atgccgggaa cactggtggc ctgaccctgc ccatttatcc agatgccgcc catggtgtag
     1861 tgcatggcgg gaaaaacctg catgggctct acgtgaggat cgacgcccaa aaacttttcg
     1921 taaatttcga ggatgccctc aagcttacgc tcaagtgttt cgcgcggaat gtgcgttaca
     1981 tccaggtaca cggcggcctt accgtgcaga ccgaggcgct cctgatagac gatgcgatga
     2041 atcgcgcgcg tagcgatatc gcgtgggacg agattgccgt attttgggta ccactcttcg
     2101 aggaaataaa accgctcgcg ctcgggaatt gttcgcgggg cgcgttggtc gttgggattt
     2161 tttggaaccc acactcggcc gccttcgcct cgcgcggact cactcatcaa gcggagcttg
     2221 tcttcgccgg gaatggcggt ggggtggacc tgaatgaact ccccgttggc gtagtcgact
     2281 ccttgttgaa aaagagcaga ctgggcggac ccggtgcaaa ctacggagtt tgtagaacgt
     2341 ccgaaaatcg cgccgattcc gccggtcgca aaaattacgg catcggcggc gtgagtggtc
     2401 acttccatgg aacgcaaatc catggcgcag ataccgcggc ccacgccaga attatcgatc
     2461 actgcggaaa gaaacgtcgc gccttcgtat tttttaactt tgccttcgga ttcgtggcgg
     2521 cgaacttgtt catcgagggc gtaaagaagt tgctggccag tggtggcacc ggcgaaagca
     2581 gtgcggtgat ataaagtgcc gccaaaacgg cggtagtcta gcaacccttc aggcgtgcga
     2641 ttaaacatca ctcccatgcg atcgagaaga tcgatgatgc cgggagcggc ttcgcacatg
     2701 cgctttacca gcggttgatt tgcgagaaag tcgccgccat aaatagtgtc gtcgaagtgc
     2761 ttggcagtgc tgtcgccttc acccttttga ttttttgcgg cattgatgcc gccctgtgcg
     2821 caaacggaat gcgaacgctt gaccggcacg atagaaaaca aatcgacagg gacacccgct
     2881 tcggcaacgc gaatggtggc catcaatccg gcaaggccgc cgccaaccac gataatttta
     2941 ggctgtttca tttggctaag acggtttcat ttagctaagt acgatgcaaa cggatgccaa
     3001 tccaggcgaa aactgagaat tatcaaaact ccgacaacac tgaatactac tccgatcaaa
     3061 acgcccaact ggccggcggc acgttgcgcc cgaacggtgg ccgcaaggcc ccacttgcaa
     3121 agaaaattcc agatgcccac gccc
//
LOCUS       DATCBB010000423         3143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_144016, whole genome shotgun sequence.
ACCESSION   DATCBB010000423 DATCBB010000000
VERSION     DATCBB010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144016"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VII37_04775"
     CDS             <1..1390
                     /locus_tag="VII37_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5069072.1"
                     /translation="NGSFWQNVQRLGFYAQDSWRVTRHLTVNYGLRYDTTFGLFNASG
                     RSQLENPAYLTLQALQIRLVRGAPHDYRGAVAPRLGIAYAPGTSGRSVIRAGIGLYYN
                     DLAQNGWVGALQGVNTAPLPCLNAVGPTPDAGCVPGAAAGGAGVDPNYKTPYALHASV
                     GFEHAFNQNWTVGASWVHEQGVHGYRNYQYVPGSTLFTPLFAAGDTADQMQFVPAVSV
                     AHSDNRSSYDALLVHVQGNVSRRFNLIANYSYSSAKTWGCILGELADYVNGVCNPLRP
                     FGPGDYGPSGEDVGSRVVVAGIFHAPGGFALSLLFQAEGARPITLTTPVDVNGLGDAA
                     NDRAVINGVQTTMDQFRGTPYVQADLRVSRPFTFHERWSVTPFVEFFNLFNRNNPGAN
                     YVTDISALPTPVNSLTNATAFCLNADCSQTKPISSPKQLLVPAGALGDFFGPGTTVGI
                     PFAAQVGLRVTF"
     gene            1569..2912
                     /gene="ffh"
                     /locus_tag="VII37_04780"
     CDS             1569..2912
                     /gene="ffh"
                     /locus_tag="VII37_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY5069073.1"
                     /translation="MFENLTEKLQRTFKNLRGQGKLTEEHLDAALVEIREALLEGDVN
                     VGVADELLKNIREKSIGTEVMMQLSPDQQVVKTVRDELTTLLGKHAKPLFASKPPSVW
                     MIVGLQGSGKTTTTGKLAKWLALHGHRPLVVSTDVYRPAAREQLSQVAKAVGTAIWQG
                     TGTNKPIEIVKGAIKEAKLSASDVILVDTAGRLHIDDSLMEELSTLKKELNPSEILFI
                     ADAMIGQDAVRSAGEFHKRLGLTGVILTKLDGDARGGAALSIGKVTGAPVKFVGLGEK
                     YDALEGFYPERIVSRVLGMGDIMSLIERAEQAVDKKAAAELERKLRTEGFTLEDFRDQ
                     LKQIRKMGPLEQLVDMLPKVGPLQSLPKDAKVDESKLKQVEAIINSMTHQERRDHNVI
                     DGKRRKRIAKGSGTTVQDVNQVIKQYMQMRQMMKQYGAMAARSKMKGLGKLAGLS"
BASE COUNT          680 a          707 c         1072 g          684 t
ORIGIN      
        1 caacggaagt ttttggcaaa atgtgcaacg gctgggattt tacgcgcaag attcctggcg
       61 agtgaccaga catctgactg ttaattatgg attgcgctac gacaccacgt ttggactttt
      121 taatgcgtcg gggcggagtc aactcgaaaa tccggcgtac ctgactttgc aagctttgca
      181 gattcggttg gtacgcggcg cgccgcatga ttatcgtgga gcagtggcgc cgagattagg
      241 gattgcttac gctccgggaa cgtcgggacg gagcgtgatt cgcgcgggga ttggtttgta
      301 ctacaacgat ctggcgcaga acgggtgggt gggcgcactg caaggggtga acaccgcgcc
      361 gctgccgtgc ttgaacgcag tgggcccgac gccggatgcg ggttgcgtac cgggagcggc
      421 ggcggggggc gcgggggttg atccgaatta caagacgcct tacgcgctgc atgcgagcgt
      481 gggatttgag catgcgttca atcaaaactg gacggtgggt gcgagttggg tgcacgagca
      541 aggggtgcac ggctaccgca attatcaata tgttcctgga tcaacgctgt tcacgccgtt
      601 gtttgccgcg ggtgataccg cggaccagat gcaatttgtg ccggcggttt ctgtggcgca
      661 ctcggataat cggtctagct atgatgcgtt gctggtgcac gtgcagggga atgtgtctcg
      721 cagatttaat ttgattgcga attattcgta ttcgtcggcg aaaacgtggg gctgcatact
      781 tggcgaattg gctgactacg tgaatggcgt ttgcaatccg ttgcgtccgt ttgggccggg
      841 agactacggg ccttcgggag aagacgtggg gagccgcgtg gtggtcgcgg ggatttttca
      901 cgcgccgggg ggatttgcgt tgagcttgtt gtttcaggcg gaaggcgcgc ggccgattac
      961 cttgactact cctgtggatg tgaatggatt gggagacgcg gcgaacgatc gcgcggtgat
     1021 caatggagtg caaacaacca tggatcaatt tcgcgggact ccttatgtgc aggcggattt
     1081 gcgggtgagc cggccgttta cgtttcatga gcgctggtcg gtaacgccgt tcgtggaatt
     1141 ttttaatttg tttaaccgca ataatccggg ggcgaattat gtgacggaca tcagcgcgct
     1201 gccgactccg gtgaatagcc tgacgaatgc gacggcattt tgtttgaatg cggattgctc
     1261 gcagaccaag ccgatcagca gtcccaaaca attgttggtg ccggctggag ccttggggga
     1321 cttttttggt ccggggacca cggtgggaat tccgtttgcg gcgcaggtag ggttgcgggt
     1381 tacgttttga atttttgaat cagggaaggc tcacacatag tttccaggtt gctggattca
     1441 ccgcttgttg ctggctgtgt ttgaccgtcc ctcagtggtc ccctagaatc aaaatgtagt
     1501 gcagtgtcgc actgttctgt aatgcagaat cctcgcttta aaattaaact ggatgccgag
     1561 tctcaaaaat gtttgaaaat ttaacggaaa aactgcagcg cacatttaaa aatcttcgcg
     1621 gacagggaaa gctcaccgaa gagcacctgg atgcggcgct ggtggagata cgcgaagcgc
     1681 tgctggaagg cgacgtgaac gttggtgtcg ctgacgagct gctgaagaat atccgcgaaa
     1741 aatcgattgg caccgaagtg atgatgcagc tctcaccgga ccagcaggtc gtgaaaacgg
     1801 tgcgggatga gttgactacg ctgctgggca agcacgccaa gccgctgttt gcctcgaagc
     1861 cgccttcggt gtggatgatt gtgggcttgc agggatccgg taagacgacg acgacgggaa
     1921 agttggcgaa gtggctggct ttgcacgggc accggccgct ggtggtttct accgacgtgt
     1981 accggcctgc ggcgcgggaa caactgtccc aggtggcgaa ggcggtgggt acggcgatct
     2041 ggcaaggtac cgggacgaac aagccgattg aaattgtgaa gggcgcgatt aaagaggcga
     2101 aactttccgc cagcgacgtg attctggtgg ataccgcggg gcggctgcat attgatgatt
     2161 cgttgatgga agaattgagc acgctgaaga aggaattgaa tcccagcgag atactgttta
     2221 tcgcggacgc gatgatcggg caggacgcgg tgcggtcggc gggagaattt cacaagcggc
     2281 tggggctgac gggcgtgatc ctgaccaagt tggacggcga tgcgcgcggc ggtgcggcgc
     2341 tctccatcgg caaagtgacc ggcgcgccgg tgaagtttgt gggcctgggc gaaaagtatg
     2401 atgcgctgga gggcttttat ccggagcgca tcgtgtcgcg cgtgctgggt atgggcgaca
     2461 tcatgtcgct gatcgagcgc gccgagcagg cggtagacaa gaaagcggcg gcggagctgg
     2521 aacgcaagct gcgcaccgaa ggctttacgc tggaagattt tcgcgatcag ctgaaacaga
     2581 ttcgcaagat ggggccgctg gagcagttgg tggatatgtt gccgaaggtg gggccgctgc
     2641 agagtttgcc gaaggatgcc aaggtggatg agagcaagtt gaagcaggtg gaagcgatta
     2701 ttaattcgat gacccatcag gagcggcggg atcacaacgt gattgatggg aagcgccgga
     2761 agcgcattgc caagggcagc ggaactacgg tgcaggatgt gaatcaggtg atcaagcaat
     2821 acatgcagat gcggcagatg atgaagcagt acggcgcgat ggccgcgaga agcaagatga
     2881 agggacttgg gaagttggct ggtttgtctt gattggaata aaggcaagac attaacagaa
     2941 ccagccgcgg gcttagccag tgcggcttgg tcaacagggt agtagcgcag ggattcgcga
     3001 tgaggccgtg ttggaatccg cgctcgccaa gtctcgaaaa aattactgca gcagcggttt
     3061 caatttagcg acccacacgg caaaaccttc catgggcacc ggttcgatgc ccacttcgcg
     3121 aagcgcggcg gccacgaaat cat
//
LOCUS       DATCBB010000424         1012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_370453, whole genome shotgun sequence.
ACCESSION   DATCBB010000424 DATCBB010000000
VERSION     DATCBB010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370453"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VII37_04785"
     CDS             complement(<1..985)
                     /locus_tag="VII37_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5069074.1"
                     /translation="MNSAAKIVLISTYELGRQPFGLASPAAWLRKRGHNVVCLDLARQ
                     ELDEAAVKSADLIAIYLPMHTATRLAAQLLPSLRGQNPRAHLCCYGLYAPMNEAYLRS
                     LGVSTILGGEFENGLVHLVDRILPANGNSAKPLPQEELLVSMERLQFEVPDRSDLPAL
                     QKYAHLIVPHDGYRIVGSTEASRGCKHLCRHCPIVPVYNGVFRIVPREIVLEDIRRQV
                     AEGAQHISFGDPDFFNGIGHAIPLVEQFHREFPEITYDVTIKIEHLRKYAEQIPKLKE
                     TGCLFVISAVESVDDAVLSALEKGHTRADFLQVAEAFRALGMTLHPTFVPFS"
BASE COUNT          232 a          312 c          270 g          198 t
ORIGIN      
        1 gagaaaaagg aacgaacgtg gggtgcaagg tcatccccaa tgcgcgaaat gcttcggcca
       61 cttgcaggaa gtcggctcgc gtatgtcctt tttccagcgc ggacagcacc gcatcgtcca
      121 ccgactcgac cgccgagatg acaaacaggc atcccgtctc tttcagtttc ggtatttgct
      181 ccgcgtattt cctcaagtgc tcaattttta tcgtgacgtc gtaagtgatt tctggaaact
      241 cgcgatgaaa ctgctccacc aacgggatcg catggccgat accattgaaa aaatcaggat
      301 cgccgaacga aatatgctgc gcgccttcgg ctacctgacg ccggatatct tccaaaacga
      361 tttcgcgcgg cacgatgcga aaaacgccgt tgtaaaccgg cacaatcggg caatgacggc
      421 agagatgctt gcatccccgg ctcgcttcgg tcgagcccac aatgcggtaa ccatcgtggg
      481 gaacgatcag atgcgcgtac ttctgcaacg caggcagatc cgagcggtct ggcacttcaa
      541 actgcaggcg ttccatcgaa accagcaatt cctcctgggg caaaggcttc gcgctgtttc
      601 cgttggccgg caatatccgg tccacaaggt ggaccaatcc attttcaaac tcgccgccaa
      661 gaatcgtcga cacgcccagg ctgcgcagat aagcctcatt catcggcgcg tacaatccgt
      721 agcagcacaa atgcgcgcgc ggattttgcc cgcgcaagga cggtagcaac tgcgcggcca
      781 gccgcgtcgc cgtatgcatc ggcaaataaa tggcgatcag atcggcggac ttgaccgccg
      841 cttcatccag ttcctgccgc gccaggtcca ggcatacaac attgtgccca cgcttacgca
      901 gccacgccgc cggcgacgcc aggccaaacg gctggcggcc aagttcgtag gtggaaatca
      961 ggacgatttt tgctgcgcta ttcacgccgc caccagtata gctggccgcg cc
//
LOCUS       DATCBB010000425         1008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_14079, whole genome shotgun sequence.
ACCESSION   DATCBB010000425 DATCBB010000000
VERSION     DATCBB010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14079"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VII37_04790"
     CDS             <1..743
                     /locus_tag="VII37_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5069075.1"
                     /translation="PAAVWMQAMILLIFAYAGFESALAPMSEAKNPRRDAAFALFVAL
                     ITCIVIYALVQWVVVGVLGPGATTDRPLAEVAREAMGNRGAALVAFGALISVYGYLSA
                     KLLAMPRITFALAKNGDLPRIFAKVSPTFRTPWFSILFYAAAVWGLAMAGSFAWNATL
                     SVVARLFYYAVVCAALIALRRKQPQAVRFRLPAGPFFALLGIGICVALATQVDLSKSL
                     ILAATLGGAVLNWVWARHGDRVKVDTGR"
     gene            781..>1008
                     /locus_tag="VII37_04795"
     CDS             781..>1008
                     /locus_tag="VII37_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY5069076.1"
                     /translation="MKSWILFLFLCSIFLVPAQDAFSATGHFTTENNLAIPMRDGVVL
                     RADILRPAAIGRKFPVLVYRTPYGKDAAQREY"
BASE COUNT          175 a          280 c          294 g          259 t
ORIGIN      
        1 cgcccgccgc cgtctggatg caggccatga tcctgttgat ttttgcctac gctggatttg
       61 aatctgcatt ggcgcccatg agcgaagcga aaaatccccg gcgggacgcg gctttcgctc
      121 tgtttgtggc actcataacc tgcatcgtga tttacgcgct ggtgcagtgg gtggtggttg
      181 gagtacttgg ccccggagcg actacagacc ggccattagc cgaagtggcg cgagaggcga
      241 tgggcaatcg cggtgcggca ttggttgcgt ttggggcgct gatttccgtt tatggttatt
      301 tgagcgccaa acttctcgct atgccccgca ttactttcgc gcttgccaag aatggcgacc
      361 tgccgcgcat atttgccaag gtaagcccca cattccgcac tccctggttt tcgattttgt
      421 tctatgccgc ggcagtatgg ggattggcaa tggccggaag cttcgcctgg aatgccaccc
      481 tgtcggtggt ggcgcgcctg ttctactacg ccgtggtttg cgctgcattg attgcgttgc
      541 gccgcaagca gccgcaggca gttcgtttcc gcctgcccgc cggacccttc ttcgctctgc
      601 tgggtatcgg tatttgcgtg gcgctcgcaa cgcaagtgga tttaagtaaa tccctaatcc
      661 tggcagctac gctcggcggg gcagtgttga actgggtatg ggcgcgtcac ggtgatcgag
      721 tgaaagttga tactggacgc tgatgatcga gctaaccgct ctgtgatacc ctcaccccga
      781 atgaaatcct ggatactgtt cctattcctc tgcagtattt ttctagttcc agcgcaagat
      841 gctttctccg cgaccggaca ttttacgact gaaaataatc tcgcgattcc catgcgtgac
      901 ggcgttgtct tacgggcaga tatcctgcgt cccgccgcga ttgggcgtaa attccccgtg
      961 ttggtctatc gcacgcctta tgggaaggac gccgcgcaac gcgagtac
//
LOCUS       DATCBB010000426         1035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_144067, whole genome shotgun sequence.
ACCESSION   DATCBB010000426 DATCBB010000000
VERSION     DATCBB010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144067"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..478)
                     /gene="tadA"
                     /locus_tag="VII37_04800"
     CDS             complement(14..478)
                     /gene="tadA"
                     /locus_tag="VII37_04800"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY5069077.1"
                     /translation="MDADIEFMQQALAEARESAASGEVPIGAVLVHDGKILARAGNRT
                     IRDCDPTAHAEIVALREAARIIGNYRLADTTLYVTIEPCSMCAGAMIQARVPRLVYGA
                     AEPRGGAVQSCFEVLSHPDLNHRVQVTSGVLASDCSYVIQAFFAARREQARQ"
     gene            698..>1035
                     /locus_tag="VII37_04805"
     CDS             698..>1035
                     /locus_tag="VII37_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069078.1"
                     /translation="MEIRRDPITQSWVVQGQKESLEDLPQKCPFDRPESEGKAILLSP
                     AEGVAQVRVLPHPDPLYRIEGQLGRLAEGIYDKMGPTGAHEVVVETPHHDRRFSQFSD
                     DEIDRVLTVWA"
BASE COUNT          245 a          305 c          276 g          209 t
ORIGIN      
        1 aacttgctga gactcactgg cgtgcttgct cacggcgcgc cgcgaagaag gcttggatta
       61 cgtagctgca atcggaagcc agaacgccgg aagtgacctg cacacgatga ttcaagtccg
      121 gatgcgaaag aacttcaaaa caggattgta ctgcgccccc gcgtggctcc gcggctccat
      181 aaaccaatcg aggcactcgc gcttgaatca tggctccggc gcacatgctg cacggttcaa
      241 tggttacgta cagggtggta tccgcgaggc ggtaattgcc aattattctc gcggcttcac
      301 gcagcgccac aatttccgcg tgagctgtgg gatcgcaatc gcgaatggtg cgattgcccg
      361 cgcgcgccag gatttttccg tcatgcacca gcaccgcgcc gataggcact tcgccgctgg
      421 cggccgattc gcgcgcttcc gccagtgcct gctgcataaa ttcgatatca gcgtccacgt
      481 tttatcgaga ataacatgga cccccgcgtc accttgacaa ttgggtggcc tcgaaacaaa
      541 ctaccgctcg aaatgtgcac atgtaccgcg catcgctgtt tatcccaagt aataatcgaa
      601 atcgcccgtt cgacagcgcg tcaaagaacg atatacttct gcctaccata acaattttta
      661 cgaaagcgca gagcgcgtag caaaccaaac ctaaaaaatg gaaattcgac gagatccgat
      721 aacgcaaagc tgggtagtcc agggccagaa agaatcgctc gaggacttgc cgcaaaagtg
      781 cccgttcgat cgtcccgaat cagaaggcaa agcgattttg ctttctcccg ccgaaggcgt
      841 cgcgcaagtg cgggtgttac ctcatcccga tcctttgtac cgcatcgagg gccagctggg
      901 tcggctcgcc gagggcatct acgacaaaat gggtcccacc ggcgcgcatg aagttgtcgt
      961 cgagactccg caccacgacc gccgattctc gcaattcagc gacgacgaaa ttgatcgcgt
     1021 gctcactgtg tgggc
//
LOCUS       DATCBB010000427         1481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_468227, whole genome shotgun sequence.
ACCESSION   DATCBB010000427 DATCBB010000000
VERSION     DATCBB010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468227"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..461)
                     /gene="rplT"
                     /locus_tag="VII37_04810"
     CDS             complement(90..461)
                     /gene="rplT"
                     /locus_tag="VII37_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY5069079.1"
                     /translation="MPRVKRGTKRRQRRKKILERASGYFLTKSKLYRAAKESVERGLK
                     FAYSGRKQKKRQYRSIWIVRIGAASRAQGISYNQFISGLKKAGVELDRKILADIATND
                     AAGFVALCETAKRANEKAKAA"
     gene            complement(630..824)
                     /gene="rpmI"
                     /locus_tag="VII37_04815"
     CDS             complement(630..824)
                     /gene="rpmI"
                     /locus_tag="VII37_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY5069080.1"
                     /translation="MPKLKTHKGAAKRFKKTATGKIKRGKAFKRHILTSKDTKRKRHL
                     DADTLVSKADSPKVKRMIPY"
     gene            complement(926..>1481)
                     /gene="infC"
                     /locus_tag="VII37_04820"
     CDS             complement(926..>1481)
                     /gene="infC"
                     /locus_tag="VII37_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY5069081.1"
                     /translation="GADGEQLGIMTPQDATRKAREQSLDLVEISATAVPPVCRIMDYG
                     KFLYEQEKKERAAKKNQKQIVLKEVKFSVNVDEHDYVTKRNHVFRFLSDGDKVKASLR
                     FRGREMAHQNLGRKVLERLVNEVGDKGIVEFRPRMEGNTVHVILAPPKQVPGAKKQET
                     GGVKKPEKPPQQPAAQSGQSKTVS"
BASE COUNT          255 a          406 c          393 g          427 t
ORIGIN      
        1 ggcctggaaa ccacaaagga cacgaaggag cacgaagtca aaaatccttc gtgaaccttg
       61 gtgtccctta tagttgagct ttatttcagc taagccgctt tggctttttc gttcgcgcgc
      121 tttgcggttt cgcacaaggc cacaaaaccc gcggcgtcat tggtcgcgat gtcagccagg
      181 atcttgcggt ccagttccac gccggctttt ttcaggccgc tgatgaactg gttatagctg
      241 atgccctggg cgcgagaagc tgctccaatg cggacgatcc agatggagcg gtactggcgc
      301 ttcttctgct tgcgtccgga gtaggcgaat ttaaggccgc gctccaccga ttctttggcg
      361 gcgcgataga gttttgattt tgttagaaaa tacccgctgg cgcgctcaag tatctttttg
      421 cggcgctggc ggcgcttagt accacgtttt acgcgaggca tgtgttgctc ccttttttgt
      481 tgccggattc ccgacggtta tcgcggctgg agggaaggtt ttaggtccct cttcgctcgc
      541 gttgggccca atctttttcg attgcgcccg cttttcaggt tcggcctcag caccggtttt
      601 caccaggcgg gccgaactct gtcccgaatt tagtacggaa tcatcctctt gacttttggc
      661 gaatcagcct tacttaccag agtatcggca tcgagatggc gtttgcgctt agtgtcctta
      721 gaggtaagaa tatggcgctt gaacgcttta ccgcgcttga tcttgccggt cgcggtcttc
      781 ttaaagcgtt tagctgcgcc tttgtgtgtt tttagtttag gcataaactc ctgccgccgc
      841 tttagcgccg gcgctattag caattagcaa aaggaactgc tggagcaacg cttctggcga
      901 attgccagtt gctatccgct atttgctagc ttacggtctt actctggccg ctctgggctg
      961 cgggttgctg cggtggtttt tccggttttt ttaccccgcc agtctcctgc ttttttgcgc
     1021 caggcacctg cttgggtggc gccaggatca cgtgcacagt attgccttcc atgcgcggtc
     1081 tgaattccac aatcccttta tcgccaacct cattcaccag gcgttccaaa acttttcttc
     1141 ccagattctg gtgggccatt tcgcgtccgc gaaagcgaag gctggctttt accttgtcac
     1201 cgtctgaaag aaaccggaaa acatggttcc gtttggtgac gtaatcgtgc tcgtcaacgt
     1261 tgacgctgaa ctttacttct ttcaggacga tctgtttctg gttctttttt gccgcgcgtt
     1321 ccttcttttc ctgctcgtag aggaacttgc cgtaatccat gatgcggcac acaggcggca
     1381 ccgcggtggc cgagatctct accaggtcaa gactttgctc acgggccttt cgggtcgcat
     1441 cctgcggtgt catgatgcca agctgctcac cgtccgctcc g
//
LOCUS       DATCBB010000428         1072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_435851, whole genome shotgun sequence.
ACCESSION   DATCBB010000428 DATCBB010000000
VERSION     DATCBB010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435851"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..>1072
                     /locus_tag="VII37_04825"
     CDS             444..>1072
                     /locus_tag="VII37_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069082.1"
                     /translation="MKRITFICMVLLVGVCAAFVPPAVAQHEHPAGDPSKLGKVSFPV
                     SCEPSLQPQFSSAVAMLHSFWYEKASDTFAAIAQKDPTCGMAYWGIAMTYYHQVWQAP
                     GPADLEAGIAAVEKAKLAGAKTQRERDYIAAIETFYKDAGQLDYRTRALAYEKAMDQL
                     QARYPDDHEAAIFYALALLATAPPTDKTYVNQKKAGAILEPLFVEQPEHP"
BASE COUNT          269 a          310 c          275 g          218 t
ORIGIN      
        1 caaaaccgcg cagacctatg agatggagcc gtccgagaaa tccaaagctc tctttcgtgt
       61 atcaatcaca aaccgtgaga ctgaatagag cacgaatgaa tctcgcccag ctgtagccga
      121 aaatctgttt acaggatgag cgaatgcctt gaccagcctc caggataact cccagattgt
      181 cggcaaacct tacgttggga ttacttcccg cttgcccgtg actcgggcgg aatcccgaga
      241 aaacgctatg atccgttgcg aactaggtca cttctgcgga taggcgaata aacatccgat
      301 gcacgctgcg aacgccaggt gcgcaagtgt cttgatgcct tacttgactt cattggggtc
      361 gcgacccaaa ataggccgca cggattttta actattcggc gtattcattc cagagggcct
      421 atttaacaac caaggagacc agcatgaagc gaatcacatt catttgcatg gtgctgcttg
      481 tgggcgtgtg cgccgcattc gtgccaccag cggttgccca acatgagcat cccgcaggcg
      541 acccgagcaa actcggcaag gtgagtttcc ccgtttcctg cgaaccatcg cttcagccgc
      601 aattcagcag cgccgtagcg atgctccatt ctttttggta cgaaaaggct agcgacactt
      661 tcgctgctat tgcacagaaa gaccccacgt gcggcatggc ctactgggga atcgccatga
      721 cgtactatca tcaggtctgg caagcgcccg gcccggctga tctagaagca ggaattgccg
      781 cggtagaaaa agcaaagtta gctggtgcca agacacagcg cgaacgcgac tatattgcgg
      841 cgatcgagac gttctacaag gacgccggcc aactcgacta ccgcactcgc gccttggctt
      901 acgaaaaggc catggaccag ctgcaagcgc gctaccctga cgaccatgag gccgcgattt
      961 tttatgcgct ggcactgctc gccacagcgc cgccaaccga caaaacgtat gtgaatcaga
     1021 aaaaggccgg cgccatcctt gagccgctat ttgtcgaaca accagagcat cc
//
LOCUS       DATCBB010000429         1214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_338253, whole genome shotgun sequence.
ACCESSION   DATCBB010000429 DATCBB010000000
VERSION     DATCBB010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338253"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VII37_04830"
     CDS             complement(<1..182)
                     /locus_tag="VII37_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069083.1"
                     /translation="MKNLLWGAAAVIGLHLAAAGNCAVYAQQKNGVPLTTGCRVEQIA
                     YQGWQAQQISNAWVKL"
     gene            456..>1214
                     /locus_tag="VII37_04835"
     CDS             456..>1214
                     /locus_tag="VII37_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069084.1"
                     /translation="MPLLPEFSRRGFIRLAAAAPLLSQIAAKNAFASAATALGKDPRQ
                     NVYTRLGVKTVINCRGTWTYLSGSLEFPEVTAAQHQASEYFVNMFELQHAASRRLAEL
                     TGAEAGMVTSGAAGAMASATAACMAGSDSKNVWQLPDTTGLKNEVIMMGGRSAFDNAI
                     RLTGAKLVLAGSPDELASAINSSTAMIYTTHLGEVLSAELSVAKKSQVPILLDDAAGI
                     PPIDNIKLYAKMGLDLYTFSGGKGLRGPQCSGLLL"
BASE COUNT          280 a          356 c          304 g          274 t
ORIGIN      
        1 atcaacttga cccaagcatt ggaaatctgc tgcgcctgcc agccctgata tgcaatttgt
       61 tcgacgcggc aaccggtggt caacggcact ccattttttt gctgcgcgta taccgcacag
      121 ttgcctgctg ccgctagatg cagcccaatc actgcagccg cgccccacaa taaatttttc
      181 atctaaaagc cgccgccctc gctgcctttc cagaattcat ggttcggcct cctctagcgg
      241 cgcgcaacta tattccgccc ttgctccaga tgctcccttg caatccggcg attcgaaacg
      301 attcgtaatg aaacaaaata aaacgctact agtcaagcaa gatgtgataa agatgccaga
      361 cgcggaaatt aaattttcca aaacgacact cgtgatcgcc atgctcccca cccgagcctt
      421 ggcaaaccga ggtttgcttt cacggaggcc cttgcatgcc cttactaccc gaatttagcc
      481 gccgcggttt cattcgcttg gccgccgcag cgcctctact tagccagatt gccgctaaaa
      541 atgcgtttgc cagcgctgca acagctcttg gtaaggatcc tcgccagaat gtttataccc
      601 gtctgggcgt aaaaaccgta atcaattgtc gaggtacctg gacatatctg agtggttctc
      661 tggaatttcc cgaagtaact gccgcgcagc atcaggcttc ggaatatttt gtaaatatgt
      721 ttgagctgca gcatgctgca agccgaaggc tcgctgagtt aacgggtgcg gaagccggca
      781 tggtcacctc gggcgccgcg ggcgccatgg cctctgcaac cgccgcgtgc atggcgggca
      841 gcgattccaa aaatgtctgg cagcttcccg acactaccgg attgaagaac gaagtgatca
      901 tgatgggcgg ccggagcgcc ttcgataatg ccatccgctt gacgggtgcg aaattggtct
      961 tggcaggatc gcccgacgag ctagccagcg ccatcaacag cagcaccgcc atgatttaca
     1021 ccacgcatct tggagaggtc ctcagcgctg aactttccgt cgccaaaaaa tcccaagtcc
     1081 cgattttgct tgatgacgcg gcgggcattc cgcctatcga taacattaag ttgtacgcaa
     1141 aaatgggcct ggacttatac acattctcag gcggaaaggg gcttcgcgga ccgcaatgca
     1201 gcggcctgtt gctg
//
LOCUS       DATCBB010000430         1030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_78507, whole genome shotgun sequence.
ACCESSION   DATCBB010000430 DATCBB010000000
VERSION     DATCBB010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78507"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..>1030
                     /locus_tag="VII37_04840"
     CDS             378..>1030
                     /locus_tag="VII37_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY5069085.1"
                     /translation="MSQHNGSSTSLRLKTGLAEMLKGGVIMDVTNSEQAEIAEKAGAV
                     AVMALERVPAQIRAEGGVARMASPKKIREIMAAVSIPVMAKCRIGHFAEAQILQELGV
                     DYIDESEVLTPADEAYHVHKHDFKVPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGD
                     VVHAVKHIRQIVQEMKILTVLGEEELYAKAKEHGAPYELIKLVAKEGKLPVPNFSAGG
                     "
BASE COUNT          258 a          295 c          280 g          197 t
ORIGIN      
        1 ccgcgtcgaa gcaggcttac caggcgatat ggggaatggt tctcaggcag gccaccatgc
       61 tttcctataa cgacgtgttc cgcttcctgg ccatcatgtt cgtggtgatg ttcccgctga
      121 ttttcctaat gcgcaaagca aaaaaaggcg gtccggtgat ggcgcattag atcatgtggg
      181 ggcggacact cttgtccgcc gcctttgact ttactttgcg gcagcagaaa aaccaacgtc
      241 aacagcggcg gacaagagtg tccgccccac ataggcattc agcccctggg tcacgtctcc
      301 cgcaatttcc accctagatc aaacccagcg ctgtaaaata atgacagcaa cgccaagcct
      361 cagaaaagag tcccaacatg tctcaacaca acggaagttc caccagtctc cgcctgaaaa
      421 ccggcctggc tgaaatgtta aaaggtggcg tcatcatgga cgtcaccaac tccgaacaag
      481 ccgaaatcgc cgaaaaggcg ggtgcagtag cggtcatggc cctcgaacgc gtcccggccc
      541 agattcgcgc tgaaggcgga gtcgctcgca tggcgtcccc gaaaaagatt cgagaaatta
      601 tggcggccgt ttccattccg gtaatggcga aatgccgcat tggacacttc gccgaggcgc
      661 agattttgca agaactcggc gttgattaca tcgatgagtc cgaagtgctc actcccgcgg
      721 atgaagcgta ccatgtgcat aagcacgact tcaaagtccc attcgtatgc ggagcgcgga
      781 atctcggcga agccctgcgg cgcattgccg aaggcgcggc catgatccgc accaagggcg
      841 aagctggcac cggcgacgtc gtccacgccg tgaagcatat tcggcagatc gttcaggaga
      901 tgaaaatcct tacggtgctg ggcgaagaag aactctacgc caaggccaaa gaacatggcg
      961 caccttatga actcataaag ctagtcgcca aggaagggaa gctcccggtc ccaaattttt
     1021 ctgccggagg
//
LOCUS       DATCBB010000431         1052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_208514, whole genome shotgun sequence.
ACCESSION   DATCBB010000431 DATCBB010000000
VERSION     DATCBB010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208514"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1052)
                     /locus_tag="VII37_04845"
     CDS             complement(<1..>1052)
                     /locus_tag="VII37_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5069086.1"
                     /translation="QAFAGTGKTTTLAAIAESLVQRKFLYLVFNRAAADEAERKMPSN
                     VTVRTAHALAFRSVGYIYKSRLAGSPWAWFPYLKEKMPRALDSVIRMGRDATSAGALI
                     IRTLDQFLRTTDGAIGAIHAPYWCDDNVAAAAGYAAEALWKNICKHNSTAPVTHDCYL
                     KLFYLQGRELAPRDWTVMLDEAQDADPVILGLLERHKGARIVVGDKYQQLNQWRGAVN
                     ALSRMRSDSAELSLTRTFRFGTGAAEWANRVLEIIGERLRIIPAPHRTTVSIEEKSVT
                     TDALLARTNAGTLDEAIHGLERGRKIHVMGGADPLIRLIRGAWDLHRGNPASGELAMF
                     ATWDELKAAANGEKNG"
BASE COUNT          208 a          311 c          284 g          249 t
ORIGIN      
        1 accgtttttc tctccgttgg ctgcagcctt gagttcgtcc caagtcgcaa acatcgcaag
       61 ttcacctgag gccgggtttc ctcggtgcag atcccacgcc cctcgtatca accttattaa
      121 tgggtcggct ccgcccataa catggatttt gcgccctcgt tcgagcccat ggattgcttc
      181 gtccaatgtc cccgcgttgg tgcgcgccag cagcgcgtcg gtcgttactg atttttcctc
      241 aatgctgacg gtggtccggt gcggtgcggg aattatgcgt aacctctcgc caatgatttc
      301 gagcacccgg ttggcccatt cggccgcacc cgtaccaaat ctgaacgttc gcgtaagaga
      361 aagttccgca ctatccgaac gcattcggct aagcgcattt accgctcccc gccactggtt
      421 gagctgctga tacttgtcgc caacgactat gcgcgcacct ttgtgacgtt caagaaggcc
      481 caaaatcact gggtccgcgt cctgcgcctc atcgagcata acggtccaat cccgcggcgc
      541 gagctcccgg ccctgcaaat agaacagctt caaatagcaa tcgtgggtga ccggcgcggt
      601 gctgttgtgc ttgcaaatgt tcttccatag ggcctcggca gcatagcccg cagctgccgc
      661 gacattgtcg tcacaccaat agggagcgtg aatcgccccg attgcgccat ccgttgttct
      721 taggaattgg tccagcgttc ggattatcaa ggctccggca gaagtcgcgt ctcttcccat
      781 acgtatgact gaatcgagag cacggggcat tttctctttc aggtatggaa accaagccca
      841 agggctgcct gccagacgtg acttgtagat gtagccgacg cttctgaagg cgagcgcatg
      901 cgcggtgcgc accgtcacat ttgacggcat tttacgttct gcttcatccg cggccgcgcg
      961 gttgaaaaca aggtaaagaa attttcgctg gacgagcgac tcagcaattg ctgcaagggt
     1021 ggtggtttta ccggtgccgg caaaggcttg aa
//
LOCUS       DATCBB010000432         1068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_273828, whole genome shotgun sequence.
ACCESSION   DATCBB010000432 DATCBB010000000
VERSION     DATCBB010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273828"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..903)
                     /locus_tag="VII37_04850"
     CDS             complement(16..903)
                     /locus_tag="VII37_04850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY5069087.1"
                     /translation="MPLRSMTGFAQVRGQVNAQLAFALSLKAVNHRFLDIHFRLPSDT
                     DSLEMKLRRVLKEKMARGHVEVILNLDRSGTNGVALNRQLIAGYVQAFRAAASEFGVK
                     GEPDLNVVLRMPGAMDAGAERSGEELEAAVMARAEEAISRLNEMREEEGRSINAELRA
                     RMAGVEKAAAGVDAHRTTVLRSYSERLQSRIQELLGTQVEKERVLQEVAVLVDRSDIQ
                     EELVRLTAHVKHFLGLLDQGGEVGKKMDFLLQEMNREANTLLSKTSGLAGEALKITEM
                     GLLMKSEIEKSREQVQNLE"
BASE COUNT          221 a          352 c          239 g          256 t
ORIGIN      
        1 ttcattcctg tttttttatt caagattctg cacctgttcg cgagattttt cgatctccga
       61 tttcatcagt agtcccatct cggtaatctt caaagcctcg ccagctaacc ccgaagtctt
      121 cgagagcaaa gtattcgctt cgcggttcat ctcctgcagc aggaagtcca tcttcttccc
      181 cacctcgccg ccctggtcga gcaagccgag aaagtgtttc acgtgcgcgg tcagccggac
      241 taattcttcc tggatgtcgc tgcgatcgac cagcacggcc acttcctgca gcacccgctc
      301 cttctcaacc tgtgtgccca acaactcctg gatgcgcgac tgcaatcgtt ccgagtaact
      361 gcggagaacg gtcgtgcgat gagcgtccac gcccgctgcc gctttttcaa cgcccgccat
      421 gcgcgctcgc aattccgcgt tgatgctccg gccttcctct tcccgcatct cattcagccg
      481 cgagatagcc tcttcggctc gcgccataac cgccgcttcc aattcttctc ccgatctctc
      541 ggctccggcg tccattgcgc ctggcatccg caaaaccaca ttcagatcgg gctcgccctt
      601 aactccgaat tcgctggcag ccgctcgaaa cgcctgcacg tagcccgcaa tcagctgacg
      661 gttcagcgcc acgccgttcg ttccggagcg atccaaattc aggatcacct ccacgtggcc
      721 gcgagccatt ttctctttca gaacccgccg cagcttcatt tccagcgagt cggtatcgga
      781 ggggaggcgg aagtgaatat ccagaaaacg gtgattcacc gccttcagcg aaagcgcgaa
      841 cgccagctga gcattcactt gccctctcac ctgcgcgaat cccgtcattg aacgaagtgg
      901 cataaagtac caggtttcaa agtttctaag gcttcaaagt ttcaaggtaa aaaccaaggc
      961 ggtctgagcc atcgcaatct cgaacgcgtt actttaaaac cttgaaacct tgaaactttg
     1021 aaacctttac ttacaaattc actcccgccc ttggtgcgtc agcctcgg
//
LOCUS       DATCBB010000433         1021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_306120, whole genome shotgun sequence.
ACCESSION   DATCBB010000433 DATCBB010000000
VERSION     DATCBB010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306120"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..>1021
                     /locus_tag="VII37_04855"
     CDS             173..>1021
                     /locus_tag="VII37_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY5069088.1"
                     /translation="MPKILRILVWLLIAAAGAIAIGAIALHRGEQINATWLVVAALCC
                     YALGYRFYSSFIAAKILVLDAQRATPAERLDNGRDFVPANKWVVFGHHFAAIAGPGPL
                     VGPVLAAQFGYLPGTLWVVAGAVFGGCVQDFVILLFSVRRDGKSLTEMARDEIGPVGG
                     FIAYTAVISIIVILLGVCALVVVNALKASPWGTFTIAMTIPIALLMGVYLRRLRPGKV
                     LESSLLGFILIMLAIWGGQWISQTPSLAHWFTLSAASLAAIIIVYGFAASALPVWLLL
                     APRDYLS"
BASE COUNT          188 a          279 c          297 g          257 t
ORIGIN      
        1 aattccccaa gatattcatc acggttctcc aaaccgattc caaaacaact tccagaaaca
       61 cagcctcggg gcaaacccga ctaccgctaa ctattgcgcg accagctgag ttcacccaca
      121 atattcccaa ccttcggtgg aatcgtcaca tcgacgggaa tccgctctct acatgccgaa
      181 gatactgcgt attcttgtgt ggttattgat cgcggcggca ggtgcgattg caatcggcgc
      241 gattgctttg catcgcggag aacaaattaa tgccacatgg ctggtggtgg cggcgctttg
      301 ctgttacgcg ctgggatacc gcttctacag cagttttatc gcggccaaaa tactcgtact
      361 cgacgcccag cgcgcaactc cggcggagcg ccttgataat ggccgcgatt ttgtccctgc
      421 gaacaagtgg gtggtctttg gacatcactt cgccgccatc gccggtccgg gtccgctggt
      481 aggccctgtg ctggccgcgc agtttgggta tttgcccgga actctgtggg tggtggcagg
      541 cgctgttttt ggcgggtgcg tgcaggattt cgtgatcctg cttttttcgg tgcggcgtga
      601 cgggaaatcg cttacggaaa tggcgcgcga cgaaattggt cccgtcggcg gattcattgc
      661 gtacacggcg gttatcagta tcatcgtgat ccttcttgga gtttgcgccc tggtggtggt
      721 gaacgcattg aaagccagtc cgtggggcac attcacgatt gcgatgacga tcccaatcgc
      781 gctgctgatg ggggtctacc tgcggcgatt gcgtccgggg aaagtgctcg agagttcact
      841 gctgggcttc attttaataa tgctcgccat ttggggtggg caatggattt cgcaaactcc
      901 ctcgctcgcg cattggttta cgctcagtgc cgccagcctc gctgcaataa tcattgtcta
      961 cggattcgcg gcgtcagctt tgccagtgtg gctgttgctg gcgccgcgcg actatctcag
     1021 c
//
LOCUS       DATCBB010000434         1621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_436003, whole genome shotgun sequence.
ACCESSION   DATCBB010000434 DATCBB010000000
VERSION     DATCBB010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436003"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VII37_04860"
     CDS             complement(<1..1187)
                     /locus_tag="VII37_04860"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069089.1"
                     /translation="MNPNLKWKALFIVAVILVCIYVLIGTPKFPTSAQDIKDNFGHQI
                     KLGLDLQGGTHLILQVQVQEAIAQETDQTVDRLIGQLRAKDIHYDEIRRADDTHILVR
                     NIDPAQLSTFRDIVTAQYSNVWDLSPAAGDPSAYVLTLRAAAIAQIQEQTMQQSLETI
                     NRRINALGLTEPTIQLHGRKDNEILVQLPGEGDPTRAKAVIQAGGQLELKLVEDPVPY
                     SSESDALVKHGGVLPPNTELVAGNSERRTATGAPETGESWYVLSRAPVVTGRDLRSAT
                     ENRNTNNPGQFEVNFTLSGDAAKRFGPFTEQNKGRQMAIVLEHRVYSAPVINGRIDDS
                     GMIEGNFSQESAHDLALVLRAGALPASIKYLEERTVGPSLGADSIRHGVQASVLSLLV
                     VMI"
     gene            complement(1241..1501)
                     /gene="yajC"
                     /locus_tag="VII37_04865"
     CDS             complement(1241..1501)
                     /gene="yajC"
                     /locus_tag="VII37_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY5069090.1"
                     /translation="MIPLGLMFAIMYFMVIMPQQRQRKKVQEMLSAIKAGDRVITSGG
                     IYGTINGLDGDSVILKIATDPQVKIRVARSAIAQVETPEDAK"
BASE COUNT          355 a          433 c          448 g          385 t
ORIGIN      
        1 aaaatcatga ctaccaaaag gctgaggacg gacgcttgta cgccgtgtct tatcgagtcg
       61 gcgccgagag aaggtccgac ggtgcgctct tcaagatatt taatggatgc tggcaaagcg
      121 ccggcgcgca gcaccaatgc gagatcgtgc gcggattcct ggctgaaatt tccctcgatc
      181 atgccggaat catcgatgcg gccgttgatc actggcgcag aataaacgcg atgttccagc
      241 acaatggcca tctgtcggcc cttattctgc tcagtgaagg gtccaaatcg cttcgcggca
      301 tcgccggaca acgtgaaatt gacttcaaac tggccgggat tgttggtgtt gcgattttcc
      361 gtggcgctgc gcaagtcacg tccggtcacc acgggcgccc ggctcaacac ataccagctt
      421 tctcccgttt cgggcgcgcc agtagccgtg cgtcgctcag agtttccggc aaccaattcg
      481 gtgttaggag gaagaacccc gccatgtttg actagtgcat cggattccga gctatatggc
      541 accggatcct cgaccagctt taattccagc tgaccgcctg cttgaatcac cgcttttgct
      601 cgcgttggat cgccttcgcc cggcagctgc acaagaattt cgttgtcctt gcggccgtgc
      661 agttgaatgg tcggttccgt caaaccgagc gcattgatgc gccgattgat ggtctcgagc
      721 gattgctgca tggtttgttc ctggatttgc gcaatcgccg cggcccgtaa tgtaagtacg
      781 taggcggaag gatcgccggc agccggagac aaatcccaca cattgctata ttgcgcggtg
      841 acgatgtcgc ggaatgtgga aagctgtgcc ggatcgatgt tgcgcaccag aatatgggtg
      901 tcgtccgcgc ggcggatttc gtcgtagtga atatccttgg cgcgcagttg tccgataagg
      961 cggtcaacgg tctggtccgt ttcctgggca atggcttcct gcacctgcac ttgaagaatc
     1021 aaatgcgtgc cgccctggag gtccaggccc agcttgatct ggtggccgaa attatccttt
     1081 atgtcttgtg ctgaggtagg gaatttcggc gtgccaatga gcacgtaaat gcaaacgagg
     1141 atgactgcaa cgatgaacag cgctttccac ttcagattgg gattcatgat taaaagatca
     1201 ggaacatggc cggatggcgg cgaaaattat tttgcatccg ttattttgca tcttccggcg
     1261 tctccacctg cgcaatggca gacctggcga cgcgaatttt aacttgcggg tccgtggcga
     1321 ttttcagaat tacggagtcg ccgtccaggc cgttaatagt gccataaatg ccgccgctag
     1381 tgatgacccg atcacccgct ttgatggcgg aaagcatttc ctgaactttt ttgcgctgcc
     1441 gctgctgcgg catgatgacc atgaaataca tgatcgcaaa catcaatccc agcgggatca
     1501 agaaactgga aaatcccgac gccgggcttg cctgaaaaaa cgtagccaaa atcatttgat
     1561 ctgtgaaaac cgatcccttc ttttaccgga aaagcccctg gttcagcccc agaaattcag
     1621 c
//
LOCUS       DATCBB010000435         1182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_78656, whole genome shotgun sequence.
ACCESSION   DATCBB010000435 DATCBB010000000
VERSION     DATCBB010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78656"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VII37_04870"
     CDS             <1..768
                     /locus_tag="VII37_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5069091.1"
                     /translation="FLFGSEKSLIRFDMSEYMEKHSVSKLIGAPPGYVGYEEGGQLTE
                     RVRRTPYSVILLDEVEKAHPDVYNILLQVFEDGQLTDGLGNTVDFRNTIVIMTSNLGA
                     RHLQKRATLGFQSAAEEGTAKSLDDLVLSEVKRVFNPEFLNRLDEIILFNPLGDEDLL
                     RIIDLLVGQTNDTLIHRQVQIVLTPEARQWVLEKTCGDRSYGARPLRRALQKYVEDPL
                     SEALIQGGLQRPATLEVYVTSDGLGFRPVVEATPVSH"
     gene            851..>1182
                     /locus_tag="VII37_04875"
     CDS             851..>1182
                     /locus_tag="VII37_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY5069092.1"
                     /translation="MSISLAARNYSCFIMGLRAGVLCLLAAVVVCLVPGARAVSLSGQ
                     AAQKGPAVVPEGTPTIERIEFQGNRRIRSETLRARIFSRPGDAVNEEALRRDFHALWN
                     TQYFEDIRL"
BASE COUNT          243 a          324 c          369 g          246 t
ORIGIN      
        1 ttcctgttcg gcagcgaaaa atcgctgatc cgtttcgaca tgtccgaata catggagaag
       61 cattccgttt ccaaactgat cggcgcgcct ccaggctacg tgggatacga agagggcggc
      121 caactgaccg aacgagtgcg gcgcacgccc tattcggtga tcttgctgga tgaagtcgaa
      181 aaagcgcatc cggatgtata caatatcctg ctccaggttt tcgaggacgg gcaattaacg
      241 gacgggctgg gaaatacggt cgatttccgc aacacgattg tgatcatgac ttccaatctc
      301 ggcgcgcggc accttcagaa gcgggccacg ctgggattcc aatcggctgc ggaagagggc
      361 acggcgaaga gtctggacga tctcgtgctc agtgaagtga agcgcgtgtt caatccggaa
      421 tttttgaatc gtctggatga aattattctg ttcaacccgt tgggagatga agacctgctc
      481 cgcatcatcg atttgttggt cggccagacc aatgacactt tgatccaccg ccaggtgcag
      541 atcgttctga cgccggaagc acgccagtgg gtcctcgaga aaacgtgcgg ggatcgcagt
      601 tatggcgcgc ggccgctgcg gcgggcactg caaaagtacg tcgaagatcc gttgtccgaa
      661 gcgctgatac aggggggcct gcagcgcccg gcaacgctgg aagtctacgt cacgagcgat
      721 gggctcggat tccgaccggt ggtcgaagca accccggtat cccactagcg aatcctacgc
      781 gcgcagggcg ggctcacact cttgagcccg tttgggggga gcgtacaatt ttgtttcggg
      841 ggaagcggcc ttgagtattt ccctggccgc aagaaactac tcgtgtttta taatggggtt
      901 gcgcgcaggc gtgctgtgtc tgcttgccgc cgtggtcgtg tgtcttgtgc ctggcgcccg
      961 ggctgtttcc ctctccgggc aggcggcgca aaaagggcct gccgtagtac cggagggaac
     1021 gcccaccatc gagcgcatcg aattccaggg caatcgaagg atccgcagcg aaacgcttcg
     1081 agcgcgtatt ttttcccggc ccggcgacgc ggtgaacgag gaggccctgc gccgcgattt
     1141 tcacgccctg tggaacacgc aatatttcga ggacatccgg ct
//
LOCUS       DATCBB010000436         1630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_208710, whole genome shotgun sequence.
ACCESSION   DATCBB010000436 DATCBB010000000
VERSION     DATCBB010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208710"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VII37_04880"
     CDS             <1..713
                     /locus_tag="VII37_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5069093.1"
                     /translation="RPDALREVLGYLPQDFGVYPHLNAIEFLEYLAAVKGVSAGAARA
                     RIRELLELVNLTGAAKRPLGGYSGGMRQRIGIAQALLNDPQLLIVDEPTAGLDPEERV
                     RFRNLLSELSGERIVILSTHIVSDIEAVATSIAILEQGYLLAHESPEALLASVAGKIW
                     EVVIPSADLPALRQKYLVSSTAHRSDGVHARVVADVAPGKSLRSLDPSLEDAYLFALA
                     SHRVSGAAGVRKPSGSAA"
     gene            710..>1630
                     /locus_tag="VII37_04885"
     CDS             710..>1630
                     /locus_tag="VII37_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069094.1"
                     /translation="MTRVADNRVTVIWAIARADFLERVRRYSFLFTLGFALYLGYLTI
                     SGNLVLQVGHVRGIFNSAWVGALLSLVASAFISLAGFYFVKNTIQRDRETRVGQILAA
                     TPLSKFTYVLGKVLSNLAVLTLMIAVLALSGIAMQLLRGEDRHIEIWKLVAPFIFLAL
                     PAMVVVAAIAVLFETIPFLRGGFGNVAYFFVWTALLSMPLAVGRHASDLIGISLVADS
                     AREAAHLTAENGGVTFSLSFGQIAAPVSTFRWDGLTWNADIFISRLAWMALAFGIMIV
                     SALLFDRFDPSRGRALREASGVGVLPSAENG"
BASE COUNT          254 a          408 c          543 g          425 t
ORIGIN      
        1 gacggccgga tgcccttcgc gaggtgctcg gttatttgcc acaggatttt ggcgtttatc
       61 cgcatctgaa cgcgattgaa tttctggaat acctcgccgc ggtgaagggc gtgagtgcgg
      121 gcgcggctcg cgcgcgcatc cgtgaattgc ttgagctggt gaacctcact ggtgcggcca
      181 agcgcccgct gggcggctac tctggcggga tgcgccagcg gattggcatc gcgcaagcgt
      241 tgctcaatga tccgcagctt ttgattgtgg atgagcccac ggctgggctc gatccggaag
      301 agcgggtgcg cttccgtaat ttgctgtcgg aactttccgg cgaacgcatt gtgattttgt
      361 cgacgcatat cgtttccgac attgaggcag tggcgaccag catcgctatt ctcgagcagg
      421 ggtacttgct tgcgcacgaa tcgccggaag cgttattggc ttcggtcgcg gggaaaattt
      481 gggaagtggt gattcccagc gctgacttgc cggcgctccg gcagaaatac ctggttagca
      541 gcaccgcgca ccgtagtgac ggggtgcacg cgcgggtggt ggccgatgtt gcgcccggta
      601 agtcgctgcg gtcgctcgat ccttctttag aagacgcgta cctgttcgcg ctggctagtc
      661 accgcgtttc tggcgctgct ggcgtacgta agccaagtgg ttctgcggcg tgacacgcgt
      721 cgccgacaat cgtgtcacgg tgatttgggc gatcgcgcgc gcggactttc ttgagcgggt
      781 gaggcgttac agttttcttt tcacgttggg cttcgcactg tatctgggtt atctgaccat
      841 ttccggcaat ctggtgctgc aagtcggaca cgtgcgcggg attttcaatt ccgcgtgggt
      901 tggtgcgttg ctgtcgctag tcgctagcgc attcatttcg ctggcggggt tttattttgt
      961 aaaaaatacc attcagcgcg atcgcgaaac gcgtgtgggc cagattctgg ctgctacgcc
     1021 gctttcgaaa tttacttatg tgctcggcaa ggtgctcagc aatctggcgg tgttgacttt
     1081 gatgattgct gtgctggcgc tgtcgggaat tgcgatgcag ctgttgcggg gagaagaccg
     1141 gcacattgag atatggaaat tagtggcgcc atttattttc ctggcgttgc cggccatggt
     1201 ggtggtggcg gcgatcgcgg tgctgttcga gacaattccc tttttgcgcg gcggctttgg
     1261 caacgtcgcg tatttttttg tgtggaccgc gctgctcagc atgccattgg ctgtcggaag
     1321 acatgcgtct gacctgattg gtatctcgct ggttgcggat agcgcgcgcg aggcggcgca
     1381 tctgacggcg gaaaatggcg gcgtcacttt ctccttgagc tttggtcaga ttgcggcgcc
     1441 ggtatcgacg tttcgctggg acgggcttac ctggaacgcg gatattttca ttagccggct
     1501 tgcgtggatg gcgctggcgt ttgggatcat gattgtttcg gcgttgctgt tcgatcggtt
     1561 cgatccctct cgaggaagag cattgcgcga ggcctccggc gtgggagtct tgccgtccgc
     1621 tgaaaacggg
//
LOCUS       DATCBB010000437         1091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_47190, whole genome shotgun sequence.
ACCESSION   DATCBB010000437 DATCBB010000000
VERSION     DATCBB010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47190"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VII37_04890"
     CDS             complement(<1..935)
                     /locus_tag="VII37_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY5069095.1"
                     /translation="MLLDGALNQLGVYQSSEIEGVVFSRDDNSLYVAESRAGSPGISA
                     LDGHDLHPLGQVPGAAIQGVDAQIEDADETQMLFAISNRGVSVVDAANPITLSAPAPM
                     FAAAPASMPSEDTSSGGTLLGVMGQNFAPDSVMKIGTQFAVNVSAPTPTMLQAASPAS
                     VSNGPANISVFSPSTNWLAIAPEAFSYGPKIVHVLPNAGVNTGGDTVQIVGYGFGSDP
                     GSVAVTIGGAAAITQAVDNISAIAPSLGLDTTYPFPLERITLQTPPGTPGWRDVVVTV
                     PSGSATAARSFQYLVSSQSYSKPAFFRFLTYDQSR"
BASE COUNT          272 a          320 c          280 g          219 t
ORIGIN      
        1 tgacggcttt gatcgtaagt tagaaaacgg aaaaacgccg gcttggaata ggactgagag
       61 ctaaccaaat attgaaatga tctcgcggcg gtagcagacc ccgacggcac cgtaacaact
      121 acatcgcgcc atcccggcgt acctggcgga gtctgcaaag taatccgttc cagcggaaat
      181 gggtaagtag tatccagtcc gagacttggc gcaatggcgg aaatattatc cactgcttgc
      241 gtaatcgccg ccgcgccgcc gattgtgacc gccacacttc caggatcgct cccgaaaccg
      301 tagccaacga tctgcaccgt atcgccccca gtatttacac ccgcattggg cagcacatgc
      361 acaattttag gcccgtaact gaatgcctcc ggcgcaatgg ccagccagtt ggtggatgga
      421 gaaaacacac tgatattcgc cggaccattt gaaacactcg caggcgaagc cgcttgaagc
      481 atcgtcgggg ttggagcact aacatttacg gcgaattgtg tacctatttt catcacggag
      541 tcgggggcga aattctgacc catcacaccc agcaaagtgc ccccgctact cgtgtcttcc
      601 gaaggcatgc tcgccggagc agccgcaaac attggggccg gagcggacag cgtgattgga
      661 ttcgcagcgt ccacaacgct cacgccgcga ttcgaaatcg cgaaaagcat ttgcgtctcg
      721 tccgcgtctt ctatttgtgc gtcaacacct tgaatcgcgg cgccaggtac ttggccgagt
      781 ggatgaaggt cgtggccatc caaagcgctg atacccggcg accccgcacg cgattctgca
      841 acgtaaagag aattgtcatc cctagaaaat acgacccctt caatttctga agactggtaa
      901 acgccaagct gattaagagc cccatcaagc agcatcaact gcgtcgcacc cttggatgtc
      961 agtaccgcag cgaaccggct agcatttgag ttggccgcaa cccaactgat tgaacccgag
     1021 cctattgact gagggcctac actcaccact ccgttgggcg cgaacgcagc aacatttccg
     1081 ctggaatcat t
//
LOCUS       DATCBB010000438         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_176578, whole genome shotgun sequence.
ACCESSION   DATCBB010000438 DATCBB010000000
VERSION     DATCBB010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176578"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..984)
                     /locus_tag="VII37_04895"
     CDS             complement(136..984)
                     /locus_tag="VII37_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY5069096.1"
                     /translation="MVYPKTFPPGFHSLEFLARRLNTVEINSSFYQPLKPEVVKLWIR
                     KVEANPQFQFTAKLHQHFTHGRVLENAEIAQFKEGLWPLHRAHKLGAVLMQFPWAFRF
                     TPENREFLIRLRRAFHEFPLVAEMRHSSWLAEEAIGTFLDYHVGFCNIDQPAYTSAMP
                     PTAYLTSGIGYVRLHGRNPKNSLGAFDRQSPARLRQHDYLYSETELEEWAKRIERINR
                     YAEATFVVFNNDAAGKSVVNALQLESILNGHPAAAPKELRRRFPMELERFGPRHFEQQ
                     CLFDAA"
     gene            complement(1104..1727)
                     /locus_tag="VII37_04900"
     CDS             complement(1104..1727)
                     /locus_tag="VII37_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEY5069097.1"
                     /translation="MLTIDREYGSGAAEIAQQIADRLGWKLWDQLLTDEVARRLECES
                     FQVERHEERRDPLYYRIFKAFLRGSYEGTLITPQMKMADAENIRRVSEQVVRKAAAEG
                     NAVIVGRGSAYYLKDEPYCFHLFIYAPFEERVRRLKLAGKSEAEATELACNVDRDRAD
                     FIKQHFGIKFPARHFFHLMVNSTMGEAAAVQTVLDALSAIQKGQISS"
     gene            complement(1749..>2044)
                     /locus_tag="VII37_04905"
     CDS             complement(1749..>2044)
                     /locus_tag="VII37_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069098.1"
                     /translation="RTQFHQSVLASHATRYDPAFRNQFAGLSRQLQHSGASAPDAQIA
                     AYGRLYRSLQVQSQTLAYIDTFMVLAVLASIMFLLAFIVRKNDPGGGGHVVAE"
BASE COUNT          415 a          542 c          644 g          443 t
ORIGIN      
        1 cgtcggacag ccacgcttcg agcggacaaa cggcggcggg attacctgca aacggatggt
       61 cggcaaaggc gtcgacgtgg taaaacggaa tcctcattcc tccactgtag cgattttcgc
      121 tccacatacg gcatgtcagg cggcatcgaa caggcattgc tgctcgaagt ggcgcgggcc
      181 gaatcgttcg agctccatgg ggaagcggcg gcgtaattcc ttgggcgcgg ccgcggggtg
      241 gccgttgaga atggattcga gttgcagtgc gttcaccaca ctttttccgg cggcgtcgtt
      301 attgaacacg acgaaggtgg cttcggcgta gcgattgatg cgctcgatgc gcttggccca
      361 ctcctccaac tccgtttcgg agtagaggta atcgtgctgg cgcaggcgcg cgggggattg
      421 gcggtcgaaa gctcccaggg aatttttggg gttgcggcca tgcaagcgca cgtagccgat
      481 gccgctggtc aaatacgcgg tgggcggcat ggcgctggtg tacgcgggct gatcgatgtt
      541 gcagaagccg acgtggtaat cgaggaaagt gccaatggct tcctcggcga gccaactgct
      601 gtggcgcatt tcggcgacca gcgggaattc gtgaaacgca cggcgtaggc ggatcaggaa
      661 ctcgcggttt tcgggggtga agcggaacgc ccaggggaat tgcatcagga cggcgccgag
      721 cttgtgggcg cggtgaagcg gccacagtcc ttccttgaat tgggcgatct ccgcgttttc
      781 taaaactcgg ccgtgcgtga aatgctgatg tagtttggcg gtgaattgga actggggatt
      841 ggcttccacc ttcctgatcc acaacttcac tacttccggc ttaagtggtt gataaaaaga
      901 gctgttgatt tcgaccgtgt tcagccggcg ggccaggaac tccagggaat gaaaacctgg
      961 gggaaaggtc ttgggataga ccacaccatt ccagtgttga taagaccagc cggcggtgcc
     1021 gacgcgaggg gaggatgaca tgggagaatc tccgttttcg ctatttcttc gctaattaag
     1081 aatagcgaat aaagggcgaa gagtcaagag gatatttggc ccttttgaat ggccgataga
     1141 gcgtccagca ccgtttgtac cgctgcggcc tcgcccatgg tggaattgac catgaggtgg
     1201 aagaaatggc gggcgggaaa cttgattccg aagtgctgtt taataaagtc cgcccggtcg
     1261 cggtcgacat tgcaggcaag ctcggttgct tccgcctcgc tctttccggc cagtttcaag
     1321 cggcgcactc tttcttcaaa aggcgcatag atgaaaaggt ggaagcaata gggctcatct
     1381 tttaggtagt acgccgagcc gcgcccgacg atcacagcat tgccctcggc ggctgctttc
     1441 ctcaccactt gctcagatac ccgccggatg ttttcggcgt ccgccatttt catctgcggc
     1501 gtaatcaaag ttccttcgta gctgccgcgc aagaaggctt tgaaaatacg gtagtagagc
     1561 gggtccctgc gttcctcgtg cctctccacc tggaagctct cgcactccag gcggcgagcc
     1621 acttcgtcgg tcagtaattg atcccataac ttccaaccga gccggtcagc gatctgctgg
     1681 gcgatttcgg cggcgccgct gccgtattcg cgatcgatgg ttagcacttt gatcataaag
     1741 ttgtctttct actctgccac gacatggcca ccgccgccgg gatcgttctt gcgaacgata
     1801 aatgccagaa ggaacatgat actcgccaac accgccagca ccatgaaggt atcaatatac
     1861 gccagggtct gcgactgcac ctgcagggac cggtagagcc gtccataggc cgcgatctgg
     1921 gcgtccggcg cgctggcgcc cgagtgctgg agttggcggc tgagtccggc gaattgattg
     1981 cggaaggcgg ggtcgtacct cgttgcatgg gaagcgagca cggactggtg aaattgcgtc
     2041 cgcc
//
LOCUS       DATCBB010000439         1016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_112376, whole genome shotgun sequence.
ACCESSION   DATCBB010000439 DATCBB010000000
VERSION     DATCBB010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112376"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..>1016)
                     /locus_tag="VII37_04910"
     CDS             complement(473..>1016)
                     /locus_tag="VII37_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069099.1"
                     /translation="PSAGRPPFDRPRTGKPSFGKPSFDKPRFEKSGPAKSRFEKPAFE
                     RSGPPKPRFEKSRFERSGPPKPRFAKPGFDKPRFEKPAFEKPRFDRSGSGRPAAPGRT
                     SDGPSRPPRGEGGLGRPFTTSSGKPRAGGARPSSKPFKPGAAGRSKPRPGGFSKSGPK
                     GSSFKGKPGGAKRPGGKKRS"
BASE COUNT          172 a          247 c          361 g          236 t
ORIGIN      
        1 tgggcattgc ggagaagttg gtgacgcaca tgccgcatgc gcgctttatt ctgctgccga
       61 tttcagacgc gacgcgcggg catgggacac atacggtggc ggcggtgtgg aaggattacc
      121 tggcggagtt catgcgggag acggagccta aaccgtaagc agattcgact tgaatggatg
      181 cgaggttccg cgcgcgtcga acggttgcgg aatggaggga gagaatttgg tgccgctttg
      241 aaagggtgct ccgcaatccg cggtcgttca aaaacgccgg cttgtttata ggtctctagt
      301 gtggtgattc aaaagttcat cggacaagtt cgttgaagtc tttggttaca tacagcaagt
      361 cagcgggttc gtgcgattgg atgagacctc ggctgccatc gattccagtg gaaacgcggc
      421 gacgcaggtt ttgcttcttc gacgtttgag gcgaactttt tgactcagga cactagcttc
      481 gcttcttccc gccggggcgc tttgctccgc cgggcttgcc cttgaagctg gagcctttgg
      541 ggccggattt cgagaagcct ccgggcctgg gtttggagcg gccagctgcg ccgggcttga
      601 acggtttgct gctggggcgt gcgccgcctg ccctaggctt gccgctactg gtggtgaagg
      661 ggcgcccgag gccgccttcg ccgcggggtg ggcgggaagg tccgtcactg gtgcggcctg
      721 gagcggcggg acgaccagag ccggagcggt cgaagcgcgg tttctcgaaa gcgggctttt
      781 caaatcgggg tttgtcgaag ccgggtttcg caaagcgggg ctttggcggg cctgatcttt
      841 cgaatcggga cttttcaaag cgaggcttcg gtggtccaga tctttcgaaa gcgggttttt
      901 cgaagcggga tttggccgga cctgattttt cgaatctggg cttatcgaaa cttggcttac
      961 cgaaactggg tttgccggtg cgcgggcggt caaatggagg gcggcctgca ctggga
//
LOCUS       DATCBB010000440         1600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_240620, whole genome shotgun sequence.
ACCESSION   DATCBB010000440 DATCBB010000000
VERSION     DATCBB010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240620"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VII37_04915"
     CDS             complement(<1..1332)
                     /locus_tag="VII37_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY5069100.1"
                     /translation="MEPAIADLMADARIELDRLTSETESRTFANTMVRLDHLTERLEY
                     AMQIIRNLEALATTPELRAANNAVQPLVSEFYSSLPLNEALWQAVLGYSRTEEAQSLS
                     GVRKRFLEKTMDNFRRHGAELDAAGKKRIAEIDVDLTKQTIRFAENVLDSTNHYEWVI
                     TDERKLAGLPESARAMARSSAASKGLPEPSWRFTLQGPSYLAVLTYLDDPELRQKVYE
                     AFIHRATEEKYDNRPILRRILELRREKARLLGYRDFADLVLEDRMARKGERAQQFLND
                     LKHKTDPQFHQENAGLLAFSGKQKLFPWDVGYYAEKQRAALYDFDEEALRLYFPLDRV
                     LSGMFEIVHRLYGIRVVEKEGVPGWDPQVKFYEIYDHGYPGRSRMIGAFYADWFPREN
                     KRGGAWMDVFITGLPRGDQADPHLGLVCGNLTPPTEDRPSLLNHREVQTIFH"
BASE COUNT          323 a          457 c          464 g          356 t
ORIGIN      
        1 gtgaaagatg gtttgcactt cgcgatggtt caacagcgac ggccggtcct cggtgggcgg
       61 agtcaggttg ccgcatacca atcccaaatg cggatccgcc tggtcgccgc gcggcaatcc
      121 ggtgataaat acatccatcc atgcaccacc gcgcttgttc tcgcgcggga accaatcggc
      181 gtaaaacgcg ccgatcatcc tgctacggcc gggatagccg tggtcgtaaa tctcgtaaaa
      241 tttcacctgc gggtcccagc ccgggacgcc ttctttttcc actacgcgga tgccgtacag
      301 ccggtgcacg atctcgaaca tcccgctgag gacgcgatcg agcgggaaat acaggcgcaa
      361 agcctcttcg tcgaaatcat agagagccgc ccgctgtttt tccgcataat atccgacatc
      421 ccaaggaaat agtttctgtt tcccggagaa cgcgagcagg ccggcattct cctgatgaaa
      481 ttgcgggtcc gtcttgtgct tcagatcgtt gaggaattgc tgcgcccgct cccccttgcg
      541 agccatgcgg tcttccagca ccaggtcggc gaagtcacgg taaccgagca ggcgggcctt
      601 ttcacggcgc agctccagaa tccgccggag gatggggcgg ttgtcatatt tttcttcggt
      661 cgcccgatga atgaaggctt cgtagacctt ttgccgcagc tccgggtcat ccagatacgt
      721 caagacggcg agatagctgg ggccttgcag cgtgaagcgc caggaaggtt cgggcagccc
      781 tttggaagct gcgctggagc gcgccatggc ccgggcgctt tccggcaacc ccgccaactt
      841 gcgctcatcg gtaatcaccc actcatagtg attggtggag tccagcacgt tctcggcgaa
      901 tcgaatggtc tgcttcgtca gatcgacgtc gatctcggca attcgttttt tgcccgcggc
      961 gtccaattcg gcgccgtgac gccggaaatt gtccatggtt ttttcaagga aacgtttccg
     1021 cacgccgctg aggctctggg cttcttcggt tcgggaataa ccgaggacgg cctgccatag
     1081 cgcctcgttc aacggcaggg acgaatagaa ttcgctcacc aggggttgga ccgcgttatt
     1141 cgcggcgcgc agctccggcg tagttgccag cgcctccagg ttgcgaatga tttgcatggc
     1201 gtattccagg cgttcggtga gatggtccag ccgcaccatg gtattggcaa aggtgcgcga
     1261 ctcggtctcg gaggtgagcc tgtcgagctc gatgcgcgca tctgccatga ggtcggcgat
     1321 ggccggctcc acatgctccg cgcgaatctg gtcgaaagga atttgaaatt gaatttgcag
     1381 cagtgggttg ccagcgcttg gagatgtcat tcggattaat cttttatttt taagatacca
     1441 gcgagcatat tttcgaagca aaatcttacg tgccaatcct gcctagccgc attgcaagct
     1501 gtcgggaaca aaccaccggt gttcctgcgt atcctagtgt gagtgaaccc cgtgccagtg
     1561 gactcagaat acgcgataca aacccaggat ctcacaaaga
//
LOCUS       DATCBB010000441         2834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_14489, whole genome shotgun sequence.
ACCESSION   DATCBB010000441 DATCBB010000000
VERSION     DATCBB010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14489"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VII37_04920"
     CDS             <1..1833
                     /locus_tag="VII37_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY5069101.1"
                     /translation="PEAHRTLAVAMNLLGGRSNTGEGGEDPATYRYEPEAANKIKQVA
                     SGRFGVTADYLVHAEELEIKMAQGSKPGEGGQLPARKVTEYIARIRHATAGTPLISPP
                     PHHDIYSIEDLAQLIHDLRAVNPYARIGVKLVSGAGVGIIAAGVAKAGADVITISGHN
                     GGTGSSPLTSIKNTGLPWEIGLRETHDTLVRAGLRSRLSLRVDGGLKFARDIILAAIL
                     GADEFGFGTASLLAIGCVMARQCHLNTCPVGIATQDEKLRARFAGKPEMVVAYFQYLA
                     EEVRNRMAQLGVRSLKELSGWYDLLETRSGMDALLIVPISESRRVAPQQEPALHPAAK
                     EASQHFAEDLGAPRKMEAIQNSDRSVGAGMSGELMRRDKTGQRLHGEIVHEYRGSAGQ
                     SFGAFLVEGMTLRLRGEANDYVGKGLSGGTISISAGRAASRRGDVLAGNTVLYGATSG
                     QLYVAGRTGERFGVRNSGALSVVEGVGQHGCEYMTGGVAVILGPLGVNFGSGMTGGLA
                     YVLRSEVEDVLHREFVTLAEIDAFEESSLRRVIEEHIYFTGSPRAAKLLLCAGTLPMV
                     RVQPGHFQGTIEATWRPMLEKLKDRDILMPVEREAQTSQAALQV"
     gene            1943..>2834
                     /locus_tag="VII37_04925"
     CDS             1943..>2834
                     /locus_tag="VII37_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5069102.1"
                     /translation="MDFDQLATFVYVAKLKSFSRAGQKVFRSQSAVSAQIRQLEQAYR
                     AKLLDRSAKSVELTPAGEVLFEYAERLLRLRDESLQGVADRGNVVQGTVLFGANEATC
                     LYLLPDIFAEFQRRYPLGHISIYRNFSHKILQRIEDGTIDVGIVTLPIKALNTKVHHI
                     YRDRLRFMVSTRNPLANRNKITLEEVAAQPLIFPRTGFTRQVLDKLFRPYRSRLHVTM
                     ELPSIGMIKRFVAADAGVSFISESFASDQVKAGEVKLLNVEGVDLWRELGLVYRRDRS
                     LPRAAQSLIAIIREHRKASES"
BASE COUNT          615 a          679 c          929 g          611 t
ORIGIN      
        1 ccggaggcgc accgcacgct ggcggtggcg atgaatttgt tgggcgggcg cagcaatacc
       61 ggggaaggcg gcgaagatcc ggcgacgtat cgctacgagc cggaagcagc aaataaaatc
      121 aagcaagtgg cgtcgggaag atttggagtg accgcggatt atttggtgca cgcggaagag
      181 ctcgagataa aaatggcgca aggctccaaa ccgggcgagg gcgggcagct cccggcgcgc
      241 aaagtcacgg aatatattgc gcggattcgt cacgccactg cgggaactcc gctgatttca
      301 ccgccgcccc atcacgatat ttacagcatc gaggatctgg cgcaactgat tcatgatttg
      361 cgcgcggtga atccttatgc gcgaatagga gtgaagctgg tttccggcgc gggcgttggg
      421 attatcgccg caggagttgc taaagcgggc gcggatgtaa tcaccatcag cggccacaat
      481 ggtggaaccg gatcttctcc gctgacgtcg ataaaaaata cgggcttgcc ttgggaaatt
      541 gggttgcgcg aaacacatga cactctggtt cgcgctggcc tccgttcgcg tctctctctc
      601 cgcgtggacg gaggcctgaa atttgcacgc gatattattt tggcggcgat actgggcgct
      661 gatgagtttg gatttgggac ggcgtcgctg ctggcgattg gttgcgtgat ggcgcggcag
      721 tgtcatctga atacctgccc agtaggaatc gccacgcaag atgaaaaact acgcgcgcga
      781 tttgcgggga agccggaaat ggtggtggcg tatttccagt atttggcgga agaagtacga
      841 aatcgcatgg cgcaattggg tgtgcgttct cttaaggaac tgtcgggttg gtatgacctg
      901 ttagaaacgc gctccgggat ggatgcgttg ctgatcgtgc cgatttccga atctcgtcgc
      961 gtggccccgc aacaggagcc cgctttgcac ccggcggcca aggaagcctc gcagcatttt
     1021 gccgaggacc tgggagctcc acgcaagatg gaagcgattc aaaattcgga tcgcagcgtg
     1081 ggcgcgggca tgagcgggga attgatgcgc agggacaaaa ccggccagcg gctgcacggc
     1141 gagattgtgc atgaatatcg cggctcagca gggcaaagct tcggcgcatt tttggtggaa
     1201 ggcatgacgc tgcgtttgcg tggcgaagcg aacgactacg ttggcaaagg attatcgggt
     1261 ggaacaattt cgatatcggc aggccgagct gcttcgcgcc ggggcgatgt gttggcgggg
     1321 aataccgtgc tgtacggagc cacgtccggg caactttatg tcgcgggccg caccggtgaa
     1381 cggtttgggg tgcgtaacag cggggcgctc tcggtggtag agggcgtggg gcaacacggt
     1441 tgcgagtaca tgaccggagg tgtggcggta atactcgggc cgctgggcgt aaattttgga
     1501 tcgggcatga cgggcggact ggcgtatgtg ttgcgatcgg aagtggaaga tgtgttgcat
     1561 cgagaatttg ttacgttggc ggagatcgat gcctttgaag agagttcgct gcggcgcgtg
     1621 attgaagaac atatttactt tacggggagt cctcgcgcgg caaaattatt gttgtgcgct
     1681 ggcacgcttc cgatggtgag ggtgcagcca gggcatttcc agggaactat cgaggcgacg
     1741 tggcggccga tgctggagaa actgaaggat cgcgatatat taatgccggt agagcgtgag
     1801 gcacagactt cgcaggcggc tttgcaggtt taggggcagg tataaggggc atttataaac
     1861 ggcaggtgta aacatttgaa aggggctacg caactgctcg cggatttcat aaatcagtta
     1921 taatcgccgc cgagcaatct ctatggattt cgaccaactc gccactttcg tatacgtcgc
     1981 aaaattgaag agtttttcgc gcgcagggca aaaagtcttc cgctcgcaat cagccgtgag
     2041 cgcgcaaatt cgccaacttg agcaagccta tcgcgccaaa cttctagatc gctccgcaaa
     2101 gtcggtggag ttaacgcccg cgggcgaagt gttgttcgaa tacgcggagc ggctgctgcg
     2161 cttgcgcgac gagtccctgc aaggcgtggc ggaccgcggg aatgtggtac agggcacggt
     2221 tttgtttggc gccaatgaag cgacctgcct ttatctgctg ccggatattt tcgcggaatt
     2281 tcagcggcgc tacccgctgg gacatatcag tatttaccgc aatttcagcc acaagatttt
     2341 gcagcgcatc gaggatggca cgatcgatgt aggcatcgtg acactgccga tcaaggcgtt
     2401 gaataccaag gttcatcata tttatcgcga ccgcttgcgg tttatggtta gcacgcgaaa
     2461 tcccctggcg aaccgcaaca aaattacatt agaagaagtg gcggcgcagc cattgatttt
     2521 tccacgcacg ggttttacgc ggcaggtgct ggacaaactt ttccggccct atcgttcgcg
     2581 actgcacgtc accatggagt tgccgagtat cgggatgatc aagcggtttg tggcggccga
     2641 cgcgggagtt tcgttcatca gcgaaagttt cgcgagcgat caagtgaagg ctggagaagt
     2701 gaagctgctc aacgtggaag gcgtggacct gtggcgggaa ttagggctgg tttacaggcg
     2761 ggaccgcagc ctgccacgcg cggcgcaatc gctcatcgct attattcgcg aacatcgcaa
     2821 agcgagtgag tctc
//
LOCUS       DATCBB010000442         1075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_208901, whole genome shotgun sequence.
ACCESSION   DATCBB010000442 DATCBB010000000
VERSION     DATCBB010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208901"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            690..>1075
                     /locus_tag="VII37_04930"
     CDS             690..>1075
                     /locus_tag="VII37_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEY5069103.1"
                     /translation="MKDSIHVSKENYLKAILEADAEGRQVIPALLAHWLEVSAPAVTM
                     ALKRLKRDGYVEVGEDGIVRLTTAGRETAYRTALRHHLIERMLNEIFGMEWYEIHEEA
                     ERLEHAVSTAFEAKLREKLGEGGACP"
BASE COUNT          238 a          287 c          327 g          223 t
ORIGIN      
        1 cggcgcttgc cggccctgtt tgcttcttca ccccggggct atactcgctg gcatgactag
       61 aagtgcagct gtcattctga tttgcgcatt ttcttgcgga tgtatgggcc aaagtacggc
      121 gcaaacgagc gcgactgagg cgcgccttgt tattggcagt cttccacccc ttacctatcc
      181 tccaatcgct ctcgcggccc atgtttccgg caatgtggat ctaagtatcg accttcaacg
      241 cgacgggacc atcagttctt tgcgcgttaa cagcggccca gaaatgctgc gtcaacccgc
      301 tctagagaat gcgcggcagc ttaagttaga gtgcggggaa tgcagtgaag tcacgaccac
      361 tttccacctc aacctcagat ttgaattagg gcctccggtg gactgctcca aaggaatcga
      421 ttcctcctac ccaaaggttg cgaaatcggg aaacaccatt acgatagcgg cccagccgat
      481 gcaaacgtgc gaccccgcga catcgatcga aacggtgcgc gctcgttcat ggaggtgcct
      541 ctttttgtgg aaatgcgggt ggcgcaaggt tgattgaacg ggcgagggca agagctcgcc
      601 gcgatgcatg atcgcagcca tcaattcagg gaatcacaca ataacgtggg gtgcggcttt
      661 caacagcaat tagactgtct agacacgcca tgaaagactc gatccacgtt tcgaaagaga
      721 attacctcaa ggccattctg gaggctgatg cggaggggcg ccaggtgatt ccggcgctgc
      781 tggcgcattg gcttgaggtg tcggcgccgg cggtgaccat ggcgctgaaa cggctgaagc
      841 gcgatggata tgtcgaggtt ggcgaggacg gcatcgtgcg tttgactacc gccggacgcg
      901 agactgcgta ccggacagcg ctgcgccacc acctcatcga acgcatgctg aacgagattt
      961 tcgggatgga gtggtacgaa attcacgaag aagcggagcg gctggagcat gcggtgtcga
     1021 cggcctttga ggcaaaactg agagaaaagc tgggtgaagg cggcgcgtgt ccgca
//
LOCUS       DATCBB010000443         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_78932, whole genome shotgun sequence.
ACCESSION   DATCBB010000443 DATCBB010000000
VERSION     DATCBB010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78932"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(700..1998)
                     /locus_tag="VII37_04935"
     CDS             complement(700..1998)
                     /locus_tag="VII37_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5069104.1"
                     /translation="MSFDKNPATHEISRRNFLFSTAAASAATLAIPSGATASAQTPAS
                     FSSLQPLGDRVHPITPDEFHGRLLRAQELMGQLNPKFDALLIGPGTSLYYFTGIHWYL
                     SERLLALIVPRSGQPILVSPAFEEGRMRESLRYPAEVRVWQEDESPTKIIAGALSDRG
                     MRTGRVGIEEMLPFTFYDHFRVAGPAYEALSADPVTIGCRGQKSVHELELMRLACTAT
                     CQVYREVFANLKEGMTEKAIADLVMAGFAKMNLHGEALVLLGAAAALPHGSKQPQTLK
                     EGEVVLIDGGCTVDGYQSDITRTGVLGKPSAKIQTVFELVRKAQDGALDAARAKKLSG
                     SVDDAARAVITNGGYGPGYKYFTHRLGHGIGLDGHEHPYLVRGSTTVLEPGMTFSNEP
                     GIYIPGEFGLRCEDDMVISAEGPAQLLSPHFQVSLEKPLA"
BASE COUNT          555 a          634 c          621 g          507 t
ORIGIN      
        1 ggacgcgctc taaccaactg agctacgggc ctgaatcgaa attagcttag cacgagaagc
       61 gcagcatttt gcaccaccat acgctcgaat cggacacgat tgcgaacata gcttccgatg
      121 aaccgcagaa cacttgagca aagcgtgagc gttatatcca tacacagtcg attgcggttc
      181 cgccgatcat cgcctccgca gtcaaattta tttttttcca ctaattcatc agcccacact
      241 tactcgcaac gccccttccg tgaacgcaag cctactataa aaaaataagc ttgaattacc
      301 gcgaataaag ttatagcttc gccaaaatta atttgcacgc gcgtccaatc cttcaagcag
      361 agaggccaga aaaatgtcca atcaaaagcg agatgacgga attcggcgaa gggattttat
      421 taacgggatg ttgattgcag caggaaccgc tgcggtaggc ggttcgtttc caatgcgcgc
      481 gatgatgtgg gtgttgcact gacaggccga atccaaccgc ccagctccgt tcgtaaaact
      541 tctttgccgg ccacacatct tccgccgggc cttctagcat ttgccatgca cgccgcgact
      601 gtaaagcaat acggatagta ggcgtagtta tcatatatgt caactatact tattgtgtat
      661 cattttgatt aaagggaggc ggatctttga gcgtacaaat caggccaaag gtttttcaag
      721 cgaaacctga aagtggggac ttaacagttg cgccgggcct tcggcggaaa tgaccatatc
      781 gtcttcgcag cgcagaccga attcgccggg gatgtagatg cccggctcgt tggaaaaagt
      841 catgccaggt tcgagtactg tggtgctgcc gcgcaccaga tacggatgct cgtggccatc
      901 cagcccgatg ccgtgaccca gccggtgcgt gaagtatttg tagccgggac catagccgcc
      961 attggtgatc accgcgcggg ccgcgtcgtc caccgagcct gagagttttt tggcgcgggc
     1021 cgcatctagc gcgccgtctt gcgctttacg aacaagctca aagaccgtct gaattttcgc
     1081 ggacggtttt ccaagcacgc ccgtgcgagt aatgtccgac tggtagccgt ccacggtgca
     1141 gccgccgtcg atgagcacca cctcgccctc cttcagggtt tgcggctgct tgctgccgtg
     1201 cggcaatgcc gcggcagcgc ccagcaaaac cagcgcctcg ccgtgcagat tcatttttgc
     1261 aaatccggcc atgaccaagt ctgcgatcgc cttttcggtc atcccctcct tgaggttggc
     1321 aaaaacttcg cgatagacct ggcaggtagc agtgcatgcc agacgcatca gttccagttc
     1381 gtgtactgat ttttgcccgc ggcagccaat ggtcacaggg tcggcagaca gcgcttcata
     1441 ggcaggcccg gctactcgga agtgatcata aaacgtgaaa ggcagcatct cttcgatgcc
     1501 cacacggccc gtgcgcatac ctcgatccga aagcgcgccc gcgataattt tggtggggct
     1561 ctcatcttcc tgccatacgc ggacttccgc cggataacgc aaagactcgc gcatgcgtcc
     1621 ctcttcaaac gcgggagaga ccagaatcgg ctggcccgag cgaggaacaa tcaaggccag
     1681 aagtctctcg cttaaatacc agtgaatccc ggtgaaatag taaagcgatg ttcccggacc
     1741 aataagcagc gcatcgaatt tcggattcag ttggcccatc agttcctgtg cgcgcagcag
     1801 tcgcccatgg aattcatccg gcgtgatggg gtgcacacgg tcacccagcg gctgcaaaga
     1861 agaaaaactt gcaggcgtct gcgcagaggc agtcgctcct gacggtatgg ccagtgtcgc
     1921 cgccgaagcg gccgcagttg agaagagaaa attacgtcga gagatttcgt gtgtagctgg
     1981 atttttatcg aagctcatag ggaaagaaga atggttgttc cacgcctacg ctgtcaagaa
     2041 tcttgatttg tgcaagcgaa agagtcgcgt aagttcagcc agcgcgccaa aaaagttgcc
     2101 tgctcagctg ctattggaga gcgagcgctg ggtgtgcccg ctctcccgtt gcgtgtgatt
     2161 aaaagctaaa gacggctccg ccctcgctgc gaacatcacg ccactcatgc tggtgcagac
     2221 ccatgaacaa aggcagatcc ggcacagcag tatttgcgcg tgtgcgatat agggattgtt
     2281 cgaaggaaaa ggatacccac ttgttaagtt tatagtt
//
LOCUS       DATCBB010000444         1018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_371062, whole genome shotgun sequence.
ACCESSION   DATCBB010000444 DATCBB010000000
VERSION     DATCBB010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371062"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..>1018)
                     /gene="dnaJ"
                     /locus_tag="VII37_04940"
     CDS             complement(8..>1018)
                     /gene="dnaJ"
                     /locus_tag="VII37_04940"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5069105.1"
                     /translation="NKGEAEVKFRECTEAYSVLSDPQKRQVYDTYGHAGLSGSNMGAD
                     FNSSVFQDFHDIFGDFFGFEDLLGGGRRGRGRTQRGSDLRYDMTLTFEEAAAGVTTKV
                     KLPKQELCTACGGTGAKKGTGATTCTTCAGRGQMAYQQGFFTITRTCPACQGAGQIIR
                     ERCLECRGNGRLEREKIIELRIPPGVDSGTRLRVTSEGEAGPNGGPAGDLYVVLDVKE
                     HQFFERRGADLYCTIPLSVAQAALGTELQVPGLSGDEKLKIPEGTQSDAVFRVKGKGL
                     PDPRGGGKGDLYYHVRVLTPTKLTREQRKLMEQLAATLKVENKPADRNSSIFEKVKDI
                     FG"
BASE COUNT          190 a          358 c          244 g          226 t
ORIGIN      
        1 acttcaacta cccaaaaatg tctttgacct tttcgaaaat cgacgagttg cgatccgccg
       61 gcttgttctc caccttcagc gtggcagcca gttgctccat caatttgcgc tgctcccgcg
      121 tcagcttagt aggagtaagc acgcgcacat ggtagtaaag atcgcctttg ccgccgccgc
      181 gcggatcagg caagcccttg cccttaacgc gaaacaccgc atcgctctgc gtgccctcgg
      241 gaattttcag cttttcgtcc ccgcttaagc ccggcacctg caactccgtc cccagcgcgg
      301 cctgcgctac gctcaaagga atcgtgcagt acagatccgc gccccgccgc tcaaaaaact
      361 ggtgctcctt tacgtcgaga acgacataca aatcgcccgc cggtccgccg ttcggaccgg
      421 cttcgccttc gcttgttacc cgcagcctcg ttcccgaatc cacacccggc ggaatccgca
      481 gctcaataat tttttcccgc tccaggcgcc catttccgcg gcattccagg caacgctcgc
      541 gaataatctg tcccgcgccc tggcacgccg ggcacgtgcg cgtgatcgtg aaaaatcctt
      601 gctggtaagc catctgcccg cggcctgcgc aggtcgtgca agtcgtggct ccggttccct
      661 tcttggctcc cgttccgccg cacgcggtgc acaattcctg cttgggcagt tttaccttgg
      721 tcgtcacgcc cgcggccgct tcctcaaacg tcagcgtcat gtcgtaacgc aaatccgagc
      781 cgcgctgcgt tcttccgcgt ccacgccgcc caccgcctaa cagatcctca aagccaaaaa
      841 aatctccaaa aatatcgtgg aagtcctgaa acacggaact gttgaagtcc gcccccatgt
      901 tcgagccgga aagccccgcg tgtccgtagg tgtcatacac ctggcgcttc tgcgggtcgc
      961 tcaatacgct gtacgcttcc gtgcattcgc ggaatttgac ctccgcttct cctttatt
//
LOCUS       DATCBB010000445         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_208932, whole genome shotgun sequence.
ACCESSION   DATCBB010000445 DATCBB010000000
VERSION     DATCBB010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208932"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..633)
                     /locus_tag="VII37_04945"
     CDS             complement(430..633)
                     /locus_tag="VII37_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069106.1"
                     /translation="MKTGQVAAAIDDYSSALRFDPKLASALYGRGLAKLKQGDKAGGD
                     TDIAAANTIQANIGDDFTRYGVR"
     gene            complement(976..1215)
                     /locus_tag="VII37_04950"
     CDS             complement(976..1215)
                     /locus_tag="VII37_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069107.1"
                     /translation="MKKTFAAVVAVATIAGSLAATPASAQRGVAPGVAAPGRYWPAPY
                     GAGPGPYDDFGSCEWVTQRFWNGNGWRIRRVRVCG"
     gene            1242..1577
                     /locus_tag="VII37_04955"
     CDS             1242..1577
                     /locus_tag="VII37_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069108.1"
                     /translation="MQPRDRRNAQDIKKFPPLHVNPPADHRIRSTECIDRGWLRVACL
                     QSRLSFIFGAAVFSVVFFRHLPELGLDVDIILLARRGLLLIVSLFALFCFTLELERVA
                     NAFGNNRQA"
     gene            2021..2275
                     /locus_tag="VII37_04960"
     CDS             2021..2275
                     /locus_tag="VII37_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069109.1"
                     /translation="MLFTQSGGSIPTPFLIILVFWLSAIFVSFKLFARTNLVVMASLL
                     VCAPSLAGALFLVLELDNPFTGLMGISSATLRSALLPLNP"
BASE COUNT          453 a          695 c          647 g          573 t
ORIGIN      
        1 tccgtttcgt gccgatactg ttgcaaaagt cgaaaatcga actgctccaa attgcgaaag
       61 ttgatttttg gacctctcta ccgctgcagc gttcttcagc gccgctacga aggtctgtgg
      121 ttgattttgg gtgaatggat gtggtccctc acgtctctgc gcccgaaacg catcagcgtc
      181 ctaaaacttt tcgctcgtca gccaaaaaag actttttcaa cactatctgc caatagcggt
      241 cgtcgaaggt ggttcagaaa acatcacgcg gcgttgagaa tgccgtggtc gccgccgcaa
      301 ttcctaaatc ggattcgcac atgcttcgac gaggggtgaa ccaatccgga gggctgccag
      361 cgtcagacgt agggcaccct cggcctgagt ctaatgtggc agtaatccga agctcgcgtt
      421 gattggtttc tatcgcacgc catagcgcgt gaagtcgtcg cctatgttgg cctggattgt
      481 gtttgccgct gcgatatcgg tatcgccgcc agctttgtcg ccctgtttga gcttggcaag
      541 cccgcgccca tagagcgcgc tcgccagctt cgggtcgaac cgcagtgcag aactataatc
      601 atcgatagcc gcggcaacct ggcccgtctt cactggccgc cagcatcaac gccgtgaacc
      661 tggaaaactg acttggcgag gtctaaaccg attgtcgtta tcatctgcat ggctcgctcc
      721 tctgaatcgt gggagcctta acagcgccca catccatggc acccatgtgc cggtggagga
      781 gccgtccaaa gcatcacaaa cggactcatg cgccgcagta tcgccttgtc gtccttcaaa
      841 tcgagttcgc cgggatggat cgctcatcgc tgcagtcttc ttcggccttg cgctttgcgg
      901 ccccggtcat ttcgacggcc tccgctcgac gtcgatggtt gctatgagcc gttcaaatct
      961 cgccgtcatc gcggatcagc cgcagacccg cacccggcgg atccgccagc cattgccgtt
     1021 ccagaaacgc tgcgtcaccc attcgcaact gccaaaatcg tcgtatggcc cgggacctgc
     1081 tccgtaaggg gcgggccaat agcggcccgg agccgcgacg cctggggcga cgccacgctg
     1141 tgcgcttgcg ggcgttgcgg cgagcgaacc ggcaatcgtt gcgaccgcga caacggcagc
     1201 aaatgtcttc ttcattctag gccctcctgt gctcgcttgg cgtgcagcca cgcgaccgcc
     1261 gcaacgccca agacatcaag aaattcccgc cgctgcacgt caatccccca gcggaccatc
     1321 gtatcaggtc aactgagtgc attgataggg gctggcttcg agttgcttgt ttgcaaagtc
     1381 gcttgagctt catttttgga gccgcggttt tctccgttgt atttttccgg caccttccgg
     1441 agcttggcct tgatgtagat attatcctgt tggcgcggcg cggcctgctg ctgattgtat
     1501 ccctgttcgc gcttttctgt tttacccttg agttagagcg ggtagccaat gcgttcggaa
     1561 acaaccggca agcttgagca cccgaagccg gagcgtttca tcagataaag ttagcttctc
     1621 aaaactgatg gtgattcacg atggcccgat acgatcggcc ggttacacca acggaaagcc
     1681 gaatcgagtc caaagcctcg tttcgcgaga aagcaggcga agaactgagg gagttcatca
     1741 tccttacggc ctatctctat gtttgcttcg cggcagtgct ttatttcaaa gctgcggttc
     1801 tacgggcgct cggcatcgcc tacgctcctc tgggcctcgc cataatcaaa gccgctatct
     1861 gcgcaaagtt catgctcatg gggcgtgtct tccaccttgg agaccgattt aaaagcttcc
     1921 acttatcgtt ccaacgctgc acaggtcgtt cgttgacacg caacgctcgc tgcaatcgcg
     1981 cgctatctcg gccttcacgg aaggcgcgcg aacccggcta ttgttgttta cgcagagcgg
     2041 cggctcgatc ccaactccat ttttaatcat tctggttttc tggctcagcg cgattttcgt
     2101 cagcttcaag ttgtttgcgc gaactaatct ggtcgtgatg gcctcgctgt tggtctgcgc
     2161 gccgtcgtta gccggcgcgc tttttctggt tctggagctg gataacccct tcacggggct
     2221 gatggggatt tcaagtgcta cactgcgcag cgcgcttttg ccgctcaatc cgtgaggttg
     2281 aggaacacaa cagggaggct ttgttgtcaa ggaaccccaa gcacggtgag gctgcggtaa
     2341 gatcatcgtc ggtcgtgctg tttccgca
//
LOCUS       DATCBB010000446         1263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_112556, whole genome shotgun sequence.
ACCESSION   DATCBB010000446 DATCBB010000000
VERSION     DATCBB010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112556"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VII37_04965"
     CDS             complement(<1..275)
                     /locus_tag="VII37_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069110.1"
                     /translation="MIRDYVSVNAGESVNIVEPGRREFLKGLLSAGALVLSVRCAPET
                     VFAAATDPTTANATAPMANAPLHPNVYLAIDTDGTVYIVAHRSEMGSG"
     gene            428..>1263
                     /locus_tag="VII37_04970"
     CDS             428..>1263
                     /locus_tag="VII37_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5069111.1"
                     /translation="MKPTRREFVSSMALAALGARYVHAAEARLPLAFSTLGCPGWTWA
                     QVLDFAEQHGFAAVELRGLQGDMNLPGRPEFAADKIPAAKRDVAAHGLKIACVSSSAE
                     MHVAEPQKRAQQLADGRSFIDLASALGAPYVRVFGNKMQGPREEVIARVSSGLHELAE
                     YAGPRNVTVIIESHGDFTDSPTLKEILTRANSEHVALLWDAHHTFDTSHEEPEFTVGQ
                     LGKWIRHTHLKDSVPDGKERRYVLTGKGDVPVKRQVEALVKINYTGFYCFEWEKVWHP
                     DLL"
BASE COUNT          265 a          307 c          404 g          287 t
ORIGIN      
        1 ccgctgccca tctcggaccg gtgcgcgacg atgtaaacag tgccgtcggt atcgatggcg
       61 agatacacat tgggatgaag cggcgcgttt gccatcgggg cagtggcatt tgcggttgtc
      121 gggtcagttg ctgctgcgaa aacagtttcc ggcgcgcagc ggacgcttaa tacgagggcg
      181 cccgcgctaa gcaggccctt caaaaattcg cgacgaccgg gctcgactat atttacggac
      241 tcgccggcgt tcacggacac atagtcacgg atcattcctg agggtctccc gattttttta
      301 cgagctagcg agctgttatt tatacgctac atcctactgc gattctcagt tcttgcaaat
      361 gcggacacac ttcctttttt ccgataccca tagtgataga attcgctcga aactgaggag
      421 tggactcatg aagcctacac gacgcgagtt cgtaagcagc atggcactgg ccgcgctggg
      481 agcacgctat gttcacgcag cggaggcgcg attgccgctg gcgttttcaa cgctggggtg
      541 tccgggctgg acctgggctc aggttctgga ctttgcggag cagcacggat tcgcggccgt
      601 ggagttgcgt ggtctgcaag gcgatatgaa tctgccgggc cggccggaat ttgctgcgga
      661 caaaattcct gctgccaagc gtgatgttgc ggcgcatgga ttgaagatcg cgtgtgtgag
      721 tagttcggcg gagatgcatg tagcggagcc gcaaaagcgt gctcaacaac tcgccgatgg
      781 acgcagcttt atcgatctgg cgtcggcctt gggcgcgcct tatgtacggg tgttcgggaa
      841 caaaatgcag ggaccgcggg aggaagttat tgcgcgggtt tcttccggac tgcatgagct
      901 tgctgagtat gcggggccgc gaaatgtgac cgtgatcatt gaatcgcacg gggacttcac
      961 cgattcgcct acgttgaaag aaatattgac ccgggcgaat tccgagcatg tggctttgtt
     1021 gtgggatgcg caccacacgt ttgatacttc gcacgaagaa ccggagttca ccgtggggca
     1081 attaggcaag tggatccggc acacacattt gaaagattcg gtgccggacg ggaaagagcg
     1141 gcgttatgtg ctgaccggaa agggtgacgt gccggtgaag cggcaggtgg aggcgctggt
     1201 taaaattaat tacaccggat tttattgctt cgaatgggaa aaagtgtggc acccggattt
     1261 gct
//
LOCUS       DATCBB010000447         1015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_436468, whole genome shotgun sequence.
ACCESSION   DATCBB010000447 DATCBB010000000
VERSION     DATCBB010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436468"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VII37_04975"
     CDS             <1..392
                     /locus_tag="VII37_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069112.1"
                     /translation="VVARGTRSVGELLCAVDNQKLELLPNINVDVKIHSNERTNALTV
                     PRAAVDVEGDHRYVFVVTKATLGKSRLTRRELQLGIADSTNFEVISGLQENEMVALPG
                     DAVLHDGMAVKIVNTDAAYVRGHPDGR"
     gene            382..>1015
                     /locus_tag="VII37_04980"
     CDS             382..>1015
                     /locus_tag="VII37_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069113.1"
                     /translation="MAASLRLTLATALMGLLTPRASMAAGQALDDVGLEAGRRAYELS
                     DYDLAVNVLQDAAAKNPRNADVLLLLARSYFELRQFDAAIANATKAVAVSPENSVYHE
                     WLGRSFGEKASRSSWFSALSLAKKAQQEFETATRLNPKNYSAFQALIEFDCAAPGIAG
                     GGEDKARPKIEQLAAMDEAEGHYAAGNCRRQKKDYPAAEAEFDKALVNAKS"
BASE COUNT          253 a          265 c          296 g          201 t
ORIGIN      
        1 aggtcgtggc acgcggaacg cgcagtgtcg gggaattgct ctgcgcggtg gataatcaga
       61 aattggaact gctgcccaat atcaacgtcg acgtgaaaat ccattcgaac gaaagaacca
      121 atgccttgac agtgccacgc gccgccgtgg atgtcgaggg cgaccataga tatgtttttg
      181 tggtaaccaa ggcaacgcta ggaaaatctc gcctgacacg gcgcgagctt cagttgggaa
      241 ttgccgattc gacaaatttc gaagtcatca gcggcttgca ggaaaacgaa atggtggccc
      301 ttccgggcga tgccgtcttg cacgacggta tggcggtaaa aattgtgaac accgatgccg
      361 cctatgtgcg aggccatccg gatggccgct agcctcaggc ttacactcgc cacagcgctt
      421 atgggattgt taaccccgag ggcatcgatg gcggccgggc aggcactgga cgatgtcggt
      481 ttggaagccg ggcgccgggc ctatgaactg agtgattacg atctggcggt gaatgttctg
      541 caggatgcag cggcgaaaaa tccgcgaaac gcagacgtcc tcttactgct ggctagaagt
      601 tatttcgaat tgcgtcagtt tgacgccgcc attgcgaacg ctacgaaggc cgtcgccgtg
      661 tcacccgaaa attcggtgta ccacgaatgg ctaggtagat cgtttggcga aaaagcctcc
      721 aggagttcat ggttttccgc gctatcgctg gccaagaaag cgcagcaaga atttgaaacg
      781 gcaactcggc tgaatccaaa aaattattcc gcgtttcaag ccctcataga atttgactgc
      841 gctgctcctg gcatcgctgg cggcggagaa gacaaggcgc ggccaaaaat cgaacaattg
      901 gcggctatgg acgaagccga gggtcactac gccgcaggaa attgtcgccg ccaaaagaaa
      961 gattaccccg ccgccgaagc cgaattcgat aaggccctgg tcaacgccaa atccc
//
LOCUS       DATCBB010000448         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_339007, whole genome shotgun sequence.
ACCESSION   DATCBB010000448 DATCBB010000000
VERSION     DATCBB010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339007"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /gene="purL"
                     /locus_tag="VII37_04985"
     CDS             complement(<1..1489)
                     /gene="purL"
                     /locus_tag="VII37_04985"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY5069114.1"
                     /translation="MATASAEIKVTAEIAAEHGLSAPEYARVQQILGRDPNITELGIF
                     SVMWSEHCSYKSSKVHLKRLPVRGKLVVQGPGENAGVVDIGDGLVAAFKIESHNHPSF
                     VEPFQGATTGVGGILRDIFTMGARPIAVLDSLRFGPITADKSAPSVSPETIAKNRRIL
                     DGVIRGIANYGNSFGVPTIGGEVCFEPCYSQNPLVNALALGIAKKEDLFFAKARGTGN
                     PVIYVGAKTGRDGIHGASLLASAEFTEESQQKRPNVQVGDPFVEKLLLEACLEAMTTG
                     AVVAIQDMGAAGLTCSTTEMASRGGTGIEIDLARVPQRESGMTPYEIMLSESQERMLL
                     VAEKGREAEVLQVFKKWGLDAVVVGEVADGGLLRVKDHGVVVAEIPAHPLAEEGPVYQ
                     RPIAQPPRREEFASNWFAFSPEKKNLTDNFKRLLTSPAISSKRWITEQYDTMVRTNTL
                     AVPGAGDAAVVRIKGTKRALALATDGNGRWCQLNPRLGAMHAVAEA"
     gene            complement(1574..2284)
                     /gene="purQ"
                     /locus_tag="VII37_04990"
     CDS             complement(1574..2284)
                     /gene="purQ"
                     /locus_tag="VII37_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY5069115.1"
                     /translation="MKFGVVVFPGSNCDHDAFYAISNVLHQPVEFIWHQSEDLANCDA
                     VILPGGFAHGDYLRTGALARFSPVMKSVTKFSKSGGLVLGICNGFQILLEAGLLPGAV
                     MRNIGLRYICRHVHIRVENTATPFTQAAAPGQILKIPIAHNDGNYTADPQTLGELQKN
                     QQIVFRYTSPDGKDDAAGNPNGSMEGIAGICNRERNVTGLMPHPERAVESALDSADGL
                     VIFRSMVESLVTSAKASA"
     gene            complement(2366..>3393)
                     /gene="glmU"
                     /locus_tag="VII37_04995"
     CDS             complement(2366..>3393)
                     /gene="glmU"
                     /locus_tag="VII37_04995"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY5069116.1"
                     /translation="RILRKSETAISAIVEESQLSGDQAEINEINSAIYCFTLEKLWPA
                     LAQVKPNNKHRELYLTDAVAVMTAKGEVVLAQVAADSREVLGCNNRADLAEVDRIFRE
                     WKRLALMDSGVTIQLPETVLVDPDVLAGEDTVIEPGVQLLGKTKIGARCTIRAGSVLS
                     DAILADDVVVEPHCLVFDSRLDDGVIVGPFARLRGGNHLRAGARVGNFVEVKKSTIGE
                     GTKAMHLTYLGDAKIGSKTNIGAGTITCNYDGFQKNPTTIGDKAFIGSDSTLVAPVRV
                     GHGAYVAAGSTITENVPADGLGIARGRQMNKAGWASKKRRELASASGAKKITGKKSRS
                     TSKKRKR"
BASE COUNT          754 a         1028 c          839 g          772 t
ORIGIN      
        1 ccgcctcggc caccgcatgc atcgcgccaa gccgcggatt taactggcac cagcggccat
       61 tcccgtcggt ggccagcgcc aatgcgcgtt tcgttccttt tatgcgtact acagccgcat
      121 cccctgcgcc cggcaccgcc agagtgttgg tgcgcaccat ggtgtcgtat tgctcggtga
      181 tccagcgctt ggaagaaatt gccggcgacg tgagcaggcg cttgaaattg tctgtaagat
      241 ttttcttttc cggggaaaat gcaaaccagt ttgacgcaaa ttcttccctc cgcggaggct
      301 gcgctatggg gcgctggtac accggccctt cttcagcgag cggatgcgcc ggaatttccg
      361 caacaacgac gccgtgatct ttgacgcgca aaagcccgcc atcggcaact tctccgacca
      421 cgactgcgtc gagtccccac tttttgaaaa cttgcaaaac ctcggcttcg cggcccttct
      481 ccgccaccaa cagcatgcgc tcctgcgatt cactcaacat aatttcataa ggagtcatgc
      541 cgctttcccg ttgcggaaca cgggcaagat caatctcaat cccagtgccg ccccgcgaag
      601 ccatttccgt ggtggagcac gtcaaacccg cagcgcccat atcctgtata gcgacgactg
      661 ccccagtggt catggcttcc aggcaagctt ccagcagcag tttctctaca aaaggatcgc
      721 ccacctgaac gttggggcgc ttttgttgcg attcctcggt gaactccgcg gacgccagca
      781 gcgatgcgcc atgaattcca tccctgccgg tttttgcgcc aacataaatc accgggttac
      841 cggtaccgcg cgccttggca aaaaacaaat cttctttctt ggcgatcccc agcgccagcg
      901 cattcacgag agggttttgc gagtagcacg gttcgaaaca aacttctccg ccgatcgtcg
      961 gcacaccaaa agaatttccg taattggcaa ttcctcgaat cacaccgtca agaatgcgcc
     1021 gattcttggc aatcgtttcg ggcgagaccg aaggcgcgga cttgtcggcc gtgatcggcc
     1081 caaaacgcaa agaatcgagc accgcaattg ggcgagcgcc catggtaaaa atgtcgcgca
     1141 aaattccgcc gacaccggtg gtggcgccct gaaatggctc cacaaaacta ggatgattgt
     1201 gggattcgat tttgaacgct gcgaccagac cgtcgccgat atccaccacc cccgcatttt
     1261 cgcctggtcc ctgaacgacg agtttccccc tcaccggcag gcgcttcaga tgcactttgc
     1321 tggatttata ggagcagtgc tcgctccaca tcacgctgaa aattcctaac tccgtgatgt
     1381 tgggatcccg tccgaggatc tgttgcacgc gcgcatactc tggagccgat agcccgtgct
     1441 ccgcggcgat ttccgccgtc actttaattt ccgcgcttgc cgtcgccaat tttcctacct
     1501 agaaaccatg gggagaaact cgatttgaat acctgaaacc atgaaatcca aaaacaattt
     1561 ctcaaaataa atcttaagcg ctcgccttcg cggaggtcac caaagattcg accatcgatc
     1621 ggaaaatcac cagcccatcc gcggaatcca gcgccgattc cacagcccgt tccggatgcg
     1681 gcatcaaccc ggtaacgttg cgttcgcgat tgcaaatgcc ggcgatgccc tccatagagc
     1741 cattgggatt accggcagcg tcatccttgc cgtcagggct ggtatagcga aaaacaattt
     1801 gctggttttt ctgtaattcc ccgagcgttt gcggatctgc agtgtaattt ccgtcgttat
     1861 gggcgatagg aattttaagt atttgtcctg gcgcggcagc ttgcgtaaaa ggcgtggcgg
     1921 tattttcaac acgaatatgc acatggcgac aaatatatcg cagaccgata ttgcgcatca
     1981 ccgcgcctgg taatagaccc gcctccagaa gaatttgaaa gccattacaa atcccaagga
     2041 ccaagccacc gctttttgaa aattttgtca ccgacttcat caccggagaa aaccgcgcaa
     2101 gcgccccggt gcgaagataa tcgccgtgag caaaacctcc cggcaaaatg accgcatcgc
     2161 aattcgctaa atcttcagat tgatgccaga tgaactcaac cggctggtgg agaacattac
     2221 ttatggcgta gaaagcgtcg tggtcgcaat tggatccagg aaagacgacg actccaaatt
     2281 tcatgcgcag gcggctccag atggcgttgc ggaagtttta ttttaacaca gcgcgtacac
     2341 cgcagccacg agccagcaca aatcttcagc gctttcgctt tttggaggtg ctccgcgatt
     2401 tcttcccggt gatttttttt gctcccgatg ccgatgccag ctcgcggcgc tttttcgacg
     2461 cccagccagc cttgttcatc tgccggccgc gagcgattcc caaaccatcc gccggaacat
     2521 tttcggtgat ggtcgagccc gcagcgacat acgcgccatg tcccacccgt actggcgcaa
     2581 cgagagtcga atcactcccg ataaaagctt tgtcgccaat cgtggtggga ttcttctgaa
     2641 agccatcgta attgcaggtg atggtgccgg cgccgatatt cgttttggat ccgatcttcg
     2701 catcgccaag gtacgtaagg tgcatcgcct tggtgccttc gccaatcgtg ctttttttca
     2761 cctctacgaa gtttcccacg cgtgccccgg ctctcaggtg gttgccgccg cgcaagcgtg
     2821 caaacggtcc aacgattacg ccatcatcca gccggctgtc gaataccagg caatgcggct
     2881 cgaccaccac atcgtcagca agaatcgcgt cactcaagac gctaccggct cggatagtgc
     2941 agcgcgcacc aatttttgtt ttgcccagca actgaacccc aggctcaatt accgtgtctt
     3001 ccccggcgag cacgtccgga tcgaccaaaa ccgtctccgg cagttggatg gttactcccg
     3061 aatccatcag cgctagccgt ttccattccc gaaatatgcg atccacttct gcaagatccg
     3121 cgcggttatt gcatcccagg acctcgcgcg aatccgcggc cacttgggcg agcaccactt
     3181 cgcccttggc ggtcatgacc gctacagcat cggtcaaata taattcgcga tgcttgttgt
     3241 tgggctttac ctgtgcaaga gcgggccata gcttttccaa cgtaaagcaa taaatcgcag
     3301 agttaatttc gttgatctcc gcttgatcgc cgctcagctg agattcctca actatggcgg
     3361 aaatggcggt ctcggatttt cgcagaattc ttc
//
LOCUS       DATCBB010000449         1450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_274529, whole genome shotgun sequence.
ACCESSION   DATCBB010000449 DATCBB010000000
VERSION     DATCBB010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274529"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VII37_05000"
     CDS             <1..538
                     /locus_tag="VII37_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069117.1"
                     /translation="KREVLLEYGSELEKMMEAETVLHWDLRAKGHKLYLEPAAKIAHT
                     NFALPGHFLAAHFYGGRVFASVRAHDANWTWPRRLLFAGGSPLIPLVRLWRILRDISQ
                     PGRAGTRSALRVLPQLLLGLSMDGAGQMLGYLFGARKETTAKLMRLEFHRNGDEGMTE
                     GKNGQLGRNPSPSIAKRS"
     gene            535..>1450
                     /locus_tag="VII37_05005"
     CDS             535..>1450
                     /locus_tag="VII37_05005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5069118.1"
                     /translation="MNATEFNPPEMSVVLVTRTPFPSIRKTIRHLSRQTFRDRLELLI
                     VVPSREALALEDSEVQGFSAVRVLEVGPIRSMPEAKLAAVRNATTPVIAFAEDHCYPE
                     PDWAEYLIKAHREKWAAVGPLLANANPNTLNSWAAYLMSHAHWNEPVKTGRVDSLPWH
                     NTSYKRQLLLEYGSELPALLAAEGFLQQRLMEGGYELYLSPTAKVSHINASRGASVIS
                     HAYVGGRVFGAKRAHYGKWSPLRRLLHIGATPLIPLVRLQRTCRDIFRIGQSKRLLPF
                     VLPALCAGLTFHALGELVGYAFGIGDAEM"
BASE COUNT          302 a          406 c          406 g          336 t
ORIGIN      
        1 caagcgcgaa gtgctacttg aatacggatc cgaactggaa aaaatgatgg aggctgaaac
       61 cgtgttgcac tgggacctcc gtgccaaagg tcacaagtta tatctggaac cagcggcaaa
      121 aatcgcgcac acaaatttcg cactacccgg ccattttctc gcggcgcatt tttatggcgg
      181 tcgcgtgttt gcatcagtgc gcgcgcacga cgctaactgg acgtggccgc gtcggctgct
      241 ttttgcaggc ggctccccct tgattcccct ggtgcgattg tggcgaatcc tacgcgatat
      301 aagtcagccc ggccgcgccg gaacgcgttc cgcgttgcgt gttctcccgc aactgctttt
      361 agggctcagc atggatgggg ctggccagat gctcggctat ttattcggcg cacgcaagga
      421 aaccacagca aaactaatgc gacttgaatt tcaccgcaat ggcgatgagg gcatgacgga
      481 agggaagaac ggtcagcttg gtcgaaatcc cagtccgtca attgccaaac ggtcatgaac
      541 gctactgaat ttaatcctcc cgaaatgtcc gtcgtgctgg tcacccgcac tccattcccg
      601 tcgatccgca aaactattcg ccacctctcg cggcaaactt tcagagaccg actggagctt
      661 ttgattgtgg tgccatcgcg cgaagcgttg gcacttgaag attccgaagt gcaagggttt
      721 agtgctgtca gggtccttga agtgggaccc atccgatcga tgcccgaggc caagctggcc
      781 gcagtgcgaa atgccacgac tccggttatt gcctttgccg aagaccattg ttacccggaa
      841 cctgattggg ccgaatatct aatcaaagcc catcgggaaa agtgggcggc agtcgggccc
      901 ttgttggcga atgccaatcc caataccttg aacagttggg cggcctattt gatgtcgcat
      961 gctcactgga atgaacccgt taaaactgga cgcgtggact cgttgccttg gcacaacacc
     1021 tcttacaagc gtcagcttct gcttgaatat ggttcggagt tgcctgcgct gctcgcggcg
     1081 gagggatttc tacagcagag actcatggag ggtggctacg agctctatct atcgccgacc
     1141 gctaaggtca gccatataaa tgcgtcgcga ggtgcctccg tgatttcgca cgcctatgtc
     1201 ggtggccgcg tctttggtgc caagcgcgca cattatggaa agtggtcgcc cttgcggcgg
     1261 ttgctgcata tcggggcaac gcccttgatc cctctggtgc gtttgcaacg cacctgccgc
     1321 gacattttca gaatcggcca aagcaaacgg ttgctccctt ttgtccttcc cgctctctgc
     1381 gccggattaa cttttcacgc gctgggcgaa ctggtcggct atgcctttgg cattggggac
     1441 gcggaaatgc
//
LOCUS       DATCBB010000450         1575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_47846, whole genome shotgun sequence.
ACCESSION   DATCBB010000450 DATCBB010000000
VERSION     DATCBB010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47846"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(502..1350)
                     /locus_tag="VII37_05010"
     CDS             complement(502..1350)
                     /locus_tag="VII37_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069119.1"
                     /translation="MEEIPHSSSTGRYLSETSVRNESHSSGVSWAAVIGGAFVTAALS
                     LILLALGTGLGFSSVSPWSNVGASASTVGTAAIVWLILMQIMSSSIGGYLAGRLRTKW
                     ANIHTDEVYFRDTAHGFLAWAVALVITAAFLASAATSMVGAAAQRSATASSAGAEREL
                     NPNEYFGDALFRSDNARPDSNGASVRGEAGRILANALRQGNVPPADKSYLAQLVASNA
                     GLSKTDAEKRVSDVFAEAQQAADTARKTIAHSLLWTFLALLIGAFCASFAATIGGRQR
                     DHVVMV"
BASE COUNT          358 a          496 c          408 g          313 t
ORIGIN      
        1 cgccgtttcg tgccaacttc cggatgggcg accaaccgtc cacaatctgg ccaacccgct
       61 caaatcccgt tccactcgcg ccgatgatgc ccaaaatttc gtacggccga atctggtttc
      121 aggcgttatt gctttaaccc cgccgccccg ttcaccacca gcgtaatggg caagccggca
      181 tcctccccaa ccggcacatc gaggagaaaa cgctggccga tcccgctgcc cactcatttt
      241 ggagcggagg ccaccgtcat agatggctga aaagcgcaat caaaatgatg atggggatgg
      301 gtacgccgag catcaaaagt aaaatagaac gcatataaag cctccttaag tcgcttaggt
      361 ccaatatttc gattcgagcg ccgcttcctc gaaccacagc cggaaggtgc acccgcccag
      421 agcagcgcgc agaggaaagg acccggcagt tgtcgccgcg cgcgctgcac tccggcagga
      481 atccggggtc gcgccgaatc gctaaaccat cactacatga tctcgttgcc tgcccccgat
      541 ggtcgcggcg aagctcgcgc aaaacgcgcc aatcaagaga gcaagaaatg tccacaacaa
      601 tgaatgcgct atcgttttgc gggcggtatc tgcggcttgt tgagcctccg caaacacatc
      661 cgacacgcgt ttctcagcgt cggttttgct gaggcccgca tttgaagcca ccagctgggc
      721 tagatagctt ttgtctgcag gaggaacatt cccctgccga agcgcattcg caagaatgcg
      781 cccagcctcg ccgcgcactg acgcgccatt cgaatctggt ctagcattgt ccgaacggaa
      841 taatgcatca ccaaaatact cgttgggatt gagttcccgc tcagcgcctg ccgatgaagc
      901 cgtggcgctc cgttgcgccg cagcacccac catggaggtg gctgcggaag ccaaaaaagc
      961 cgccgtgatt acgagggcta ctgcccacgc caggaacccg tgcgcggtgt cgcggaaata
     1021 cacttcatct gtgtgaatat tggcccattt cgtacgcagt cggcccgcca ggtagccacc
     1081 gatggacgaa ctcatgattt gcataaggat cagccacacg atggctgcgg tgccgaccgt
     1141 cgaggcggac gcgccgacat tcgaccacgg cgataccgag gaaaaaccaa gtccagtacc
     1201 cagcgcaagc aggataagcg acaacgcggc ggtcacaaat gcgccaccga tcactgccgc
     1261 ccacgacaca cccgacgagt gtgattcatt tctgactgaa gtctcgctga gatagcggcc
     1321 ggtcgacgat gaatgtggta tttcttccat tcgattctct cccttgttaa ccagggtttc
     1381 cgttttggat tcctgaccag catcgattgt acaagcaaac gcctccgttg gagccgtttt
     1441 tcatcctttc catcctgagc ggatccggca gcccaggaaa ggggccagct ctaccttctc
     1501 cgggtacgga aaactcggaa gtgcctcgtc caaacgattt ctcatacacc tctccgtccc
     1561 tctctttcct tcgac
//
LOCUS       DATCBB010000451         1263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_436793, whole genome shotgun sequence.
ACCESSION   DATCBB010000451 DATCBB010000000
VERSION     DATCBB010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436793"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VII37_05015"
     CDS             <1..768
                     /locus_tag="VII37_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY5069120.1"
                     /translation="KAATLEARVKPDAIAALCAGIAGVGAARAAEKMRALLVAAFPSV
                     AMKVCTDLEIALAATGAGPAIVLIAGTGSAAMGRGVNGEVRRAGGLGPQVGDQGSARD
                     VGRKAVAAARLERDRSGEESALGKQLKRQLGVSNWREFETRISSTPLEQVYPQLFPVV
                     ANAADARDPMAREILRAAANDLAAVAKTLADDLNLQQVPFRLAKTGGMIGHCAFFDDE
                     LDSCLREATPTAKIGLLPISPAHAAALLALELSLEAD"
     gene            781..>1263
                     /locus_tag="VII37_05020"
     CDS             781..>1263
                     /locus_tag="VII37_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069121.1"
                     /translation="MADANDSTRSNDTRDAPGTQRALAATGEELAALLHSDDEDVLFA
                     LLQNPALAETEIALLLARKNLPAALIEEICQRREWLKTYAIKKALACHPHTPRLVTMR
                     LLRKLYLMDLVQIALLPGVSAELKRNAEDQLLSRLQQLPLGQKITLGRRGPARVAGLL
                     L"
BASE COUNT          271 a          356 c          393 g          243 t
ORIGIN      
        1 aaagccgcaa cccttgaggc gcgagtgaag cccgacgcga ttgccgcgtt gtgtgctgga
       61 atcgcaggcg tgggggcagc aagggctgct gagaaaatgc gcgcattact ggtggcggca
      121 tttcccagcg tggccatgaa agtttgtacg gatttggaaa ttgcgctggc cgccacaggt
      181 gcgggacctg ccatcgtgtt gatcgccggc accgggtccg cagccatggg gcgcggcgta
      241 aatggagaag tccgccgtgc gggcggtttg ggtcctcaag tcggtgacca aggcagcgcg
      301 cgcgatgttg gcagaaaagc tgttgctgcg gcgcgactgg aacgcgacag aagcggcgaa
      361 gaaagcgccc tgggcaagca gctaaagcgc caacttggtg tttccaactg gcgtgagttc
      421 gaaactcgta tcagcagcac tccgctcgaa caggtctatc cgcaactgtt tccggtggtc
      481 gctaacgccg ccgacgctcg cgacccgatg gcccgcgaaa ttttgcgagc agccgccaac
      541 gatcttgcgg cggtggcgaa aactcttgcc gatgatctta atttgcagca agtcccattt
      601 cgcctggcaa aaacaggcgg aatgatcggg cactgcgcgt tttttgatga tgagctggac
      661 tcgtgcctgc gcgaagccac gccaaccgcg aaaattggat tattgccgat ttcgccggcg
      721 catgccgccg cattgctggc gctggagtta tcgctggaag cggactgaca aaaaaattca
      781 atggctgacg caaacgactc aacgagaagc aatgacaccc gtgatgcgcc cggcacgcaa
      841 cgtgcgctcg cggcgaccgg tgaggaactc gcagcgctgc ttcacagtga tgatgaagac
      901 gtgctttttg ctttgctgca aaacccggcg ctggcagaaa ccgaaatcgc tctactgctg
      961 gcgcgtaaga atttgcccgc cgccctcatc gaagaaattt gtcagcgtag agagtggctt
     1021 aaaacctacg cgataaaaaa agccttggcc tgccatccgc acacccctcg cctcgtcacg
     1081 atgcggctgc tgcgcaaatt gtacttgatg gacctggtgc agatcgctct gctgccgggc
     1141 gtttccgcgg aattaaaacg caacgccgag gatcaactgt tgtcgcgatt gcagcagctt
     1201 ccgctgggcc aaaaaatcac gctggggcgg cgaggccccg cgcgcgtcgc gggattgttg
     1261 ctg
//
LOCUS       DATCBB010000452         1050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_241141, whole genome shotgun sequence.
ACCESSION   DATCBB010000452 DATCBB010000000
VERSION     DATCBB010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241141"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..>1050
                     /gene="prfA"
                     /locus_tag="VII37_05025"
     CDS             34..>1050
                     /gene="prfA"
                     /locus_tag="VII37_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY5069122.1"
                     /translation="MAVTLSERLDQLGARYDELTRELSTSEVVSDTSHFQKVAKQHAE
                     LEQIVEKHREFKQIEKDLAGAHQMIGEAEDSEMKQLAYDEEKQLVARKETVERELKLL
                     LLPKDPNDEKSVIVEIRAGTGGDEAALFAAELFRMYSRYAETLGWRVEVLESSPSSLG
                     GLKEIVAAIQGYKVYSKLKYESGVHRVQRVPATEQQGRIHTSAATVAVLPEADDIDIK
                     IEAKDLRIDTFCSSGPGGQSVNTTYSAVRITHLPSGLVVSQQDEKSQIKNRAKAMRVL
                     RARLYEQELEKQQAAILAERRGQIKTGDRSEKIRTYNFPQNRVTDHRIGLTLHQLTDV
                     MDGKL"
BASE COUNT          271 a          253 c          310 g          216 t
ORIGIN      
        1 gattcatctt aaagttaaaa ttcgtgagga tttatggctg taacactatc cgagagatta
       61 gatcaactgg gcgcacgcta cgacgagctc accagggagc tttccacctc cgaagtggtg
      121 tccgacactt cccattttca aaaagtagcc aagcagcacg cggagctgga acagattgtt
      181 gagaagcatc gcgagttcaa gcaaattgag aaggatttgg ccggcgctca ccaaatgatt
      241 ggcgaagcgg aagattcgga aatgaagcaa ttggcttacg acgaagagaa gcaattggta
      301 gcacgcaaag aaaccgtcga acgtgagctg aaactgttgc tgcttcccaa ggatcccaac
      361 gatgagaaga gcgtcatcgt ggaaattcgc gcgggcacgg gcggcgacga ggcggcgctt
      421 tttgcggcgg aactttttcg catgtattcc cggtacgcgg agacactggg ctggagagtg
      481 gaagtgctgg agagctctcc atcttcgctg ggcggattaa aggaaattgt ggcggctatt
      541 cagggctaca aagtttattc caagctgaaa tatgaaagtg gagttcatcg cgtgcagcgc
      601 gtgccagcta ccgagcagca agggcgcatc catacttcgg ctgcgaccgt tgcggtgctt
      661 ccggaagccg acgacatcga cattaaaatt gaggccaagg atttacgcat cgatacgttt
      721 tgttcttccg ggcctggcgg gcagtcggtt aacacgacct attccgcggt gcgcatcacg
      781 cacttgccga gcgggctggt ggtttcgcag caggacgaaa aatcgcagat caagaatcgc
      841 gccaaggcta tgcgggtgtt gcgagcgcgg ctttacgagc aggagctgga aaagcagcaa
      901 gcagccatcc ttgcggaacg ccgcggacag atcaaaaccg gagatcgctc ggaaaagatt
      961 cgcacttata atttcccgca gaatcgcgtt acggatcacc gtattggctt gacgctacac
     1021 caactgaccg atgtgatgga cggcaagctg
//
LOCUS       DATCBB010000453         1773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_79618, whole genome shotgun sequence.
ACCESSION   DATCBB010000453 DATCBB010000000
VERSION     DATCBB010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79618"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            668..1642
                     /locus_tag="VII37_05030"
     CDS             668..1642
                     /locus_tag="VII37_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY5069123.1"
                     /translation="MKKVAQIIATTTLVLFLALVPAAAQDYPARTTTIICPYAPGGAT
                     DILARMLARGLEERLGKSFVVENRPGAGTVLAAHIVAKAQPDGYTLLMGTSTPLAINA
                     TLHRKLPYDPAKDFVPLALIANVPFVLVVTPSLPVKSVADLIKYAKDNPGKLSFGTSG
                     PGSPHHLYMELFKTMTGTEMVHVPYKGSVPALTDVIAGRIPVMFVDLAPALQFIKNGN
                     VRAIGVSSITRVPQLPEVPPVADSVPGFEAVAWQMLVAPNGTPKPIVDRLHAELKSIE
                     AIPEFKEQVAKLGTVPVDTPSVAELQAYVKSEIVRWGKIVEKSGAAMD"
BASE COUNT          432 a          515 c          490 g          336 t
ORIGIN      
        1 cgacgcgcgg gcccgcgaac cagtctctgt cgcctatcga accggtctcc agcaagccga
       61 aacggaaatt ggaaaacaac gagcagagac tggggctaga aaagcgcctt tcctgcaccc
      121 gccaccttcg aatttgtggc cagagactgg gtcacgccag cctaacccac cggaatgtcg
      181 gtcgttctca cgcaccggga aatcgcggcg cagagactgc actggctggc tgacgatgca
      241 gtcgcattcg aaccagtctc caaaaccaaa tatccgaata acagggaaaa atacagggaa
      301 aaccggaaaa ttaagcctgt agcggagacg caatcagcaa aaaaacccag catccacgcg
      361 ggttttcagc gaaattcatc gtgaaaaata acagggaacg atttccgcat aacagggaaa
      421 ctatgagcaa ataacaggga aaaccccttc aatttcccaa tccgatcctt gttcccttta
      481 tcgtatatca gcccatcttt tggcgtggcg aaatcgatac tggcctgacg gcttttttga
      541 accaacggaa tagagattac tgccgataag gctttcggta cacacacggc caataatcgg
      601 taaatatagg tttgcaacgg ggcacaaagc taaaggtact gtatcgatag aacttcggga
      661 ggcagcgttg aaaaaagtag cccagattat tgcgacgaca acgctcgttt tatttctcgc
      721 acttgtgcct gcggccgcac aggattaccc agcgcggaca acgaccatca tctgtcccta
      781 tgcccccggc ggcgccaccg acattctcgc ccgcatgctt gcgcgcgggc tggaagaacg
      841 tctgggcaaa agcttcgtgg tcgaaaaccg gccgggcgcg ggcacggtgc tggccgcgca
      901 catcgtggct aaagcgcagc cggatggtta caccttgctg atgggcacca gcacgccgct
      961 cgccatcaac gccacgctgc accggaagct gccctatgac ccggccaagg atttcgtgcc
     1021 gctggcgctg atcgccaatg tgcccttcgt gctggtggtc acgccatcgc tgccggtgaa
     1081 atcggtcgct gatctgatca aatacgccaa ggacaatccg ggcaagttgt cgttcggcac
     1141 tagtggaccg ggttcgccgc accatctcta catggaattg ttcaagacca tgaccggcac
     1201 cgaaatggtg cacgtgccct acaagggcag cgtgccggcg ctgactgacg tcattgccgg
     1261 ccgcatcccg gtcatgttcg tggatctggc accggcgctg cagttcatca aaaacggcaa
     1321 tgtccgtgcc atcggcgttt cgtcgattac gcgggtccca cagcttcccg aagtgccacc
     1381 ggtcgccgat agtgtgcccg gcttcgaagc ggtggcgtgg cagatgctgg tagcgccgaa
     1441 cggcacgccg aagccgatcg tcgatcggct gcatgccgag ctcaagagca tcgaggcaat
     1501 accggaattc aaggagcagg tcgccaagct aggcacggtt ccagtcgaca cgccatcggt
     1561 cgcggagctg caagcttacg tgaaatctga aatagtccgc tggggcaaga ttgtcgagaa
     1621 atctggcgcc gcgatggact gaccttcgac caatctcatc atcgcggata acgaaaggaa
     1681 ttggctgtgc caatcatcga tattcatacc cacatgctta cgcccgaatg gatcgagctg
     1741 ctgcgcggca acggcggcgg caaatacagc gtg
//
LOCUS       DATCBB010000454         1648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_371598, whole genome shotgun sequence.
ACCESSION   DATCBB010000454 DATCBB010000000
VERSION     DATCBB010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371598"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VII37_05035"
     CDS             <1..648
                     /locus_tag="VII37_05035"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069124.1"
                     /translation="VGKSGKSTNYVVGGSLATQLALANLGCIAVHVSGSRSSSLRKPD
                     WVCFDMDPQSGKFSDAARAGLRLKEVLDVLKLESFPKTSGSRGLHVFVPIKPDTDVDK
                     VLFFSESLVARLAAQFPKDLTVEHSIAARAQRVYLDPYRNGFGQTVVAPYSVRRRPKA
                     PFSTPLSWSETNPALDPSEFNLGNYPKRLSAADPWQDFFRSRQPLTAAINALKKI"
     gene            700..1419
                     /locus_tag="VII37_05040"
     CDS             700..1419
                     /locus_tag="VII37_05040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5069125.1"
                     /translation="MNKHSHATDTSQRQAVPLPPMLFDLDGTLVDSAYQHVLAWHETF
                     ADFRMDIPNWKIHRRVGMSGSFFLPNLLREIGHSTNPAVIKRLEKSHAKYFTRKIKTV
                     RILPGACELLQHLSKIGLPWTIATSGERAQTEKLVKGLRLPKSLPVVTGDDVKNAKPA
                     PDIFLFAAEKLGVAISDCIIVGDSPWDLLAGRRMKALGVGLLCGGYAKEELERAGAHL
                     VYDDPAELLKNLNELGINEEK"
     gene            1458..1616
                     /locus_tag="VII37_05045"
     CDS             1458..1616
                     /locus_tag="VII37_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069126.1"
                     /translation="MPSKQSAAARKNIKKAANAARKKRTIAHLPKSVRTALGKKGAQA
                     AKRNRSKR"
BASE COUNT          403 a          516 c          382 g          347 t
ORIGIN      
        1 gtaggcaagt ccggcaaatc cacaaattac gttgttggcg gctctctcgc cacgcaactc
       61 gcgctcgcta acctcggttg catcgccgtt cacgtatccg ggagccgctc atcttccttg
      121 cgcaaacccg actgggtttg cttcgatatg gacccacaat caggaaaatt cagcgatgcc
      181 gcacgcgccg gcctgagact aaaagaagtt cttgatgttt tgaaactgga atcgtttccc
      241 aaaacttctg gcagccgcgg ccttcacgtc tttgttccca tcaagccgga taccgacgta
      301 gataaagttt tgttcttttc ggaatcactg gtcgctcgcc tcgcagcgca attccccaaa
      361 gatctcaccg tggagcattc catcgccgcc cgcgcgcaac gcgtttatct cgatccttac
      421 cgcaacggtt ttggccagac ggtagtagct ccatactccg tgcgccgccg ccccaaagcg
      481 ccattttcca ccccactctc gtggtcggaa actaacccgg cgctcgatcc ttccgaattc
      541 aaccttggca attaccccaa gcgcctgagc gccgctgatc cttggcagga tttttttagg
      601 agccgacagc ctctaaccgc ggcaataaac gcccttaaaa aaatctgatg acatgattgc
      661 cgtcattttt ggacgaatgg ttaaagaaaa cgagtctcga tgaacaaaca ctctcacgca
      721 accgatactt cgcaacgcca ggctgtcccg ctccctccca tgcttttcga tctggatggc
      781 acgctggtcg acagcgccta tcagcatgtg ctcgcctggc atgaaacctt cgccgacttc
      841 cgcatggaca ttcccaactg gaaaattcac cgccgcgtag gcatgagcgg ctcgtttttc
      901 ctgcccaatc ttcttcgcga aataggccac tccacaaatc ccgcggtcat caagcgcctt
      961 gagaaaagcc acgcaaaata tttcacccgc aaaataaaaa ccgttcgcat ccttccaggc
     1021 gcatgcgaac tcttgcagca cctctccaaa atcggcttgc cttggactat cgccaccagt
     1081 ggtgagcgcg ctcaaaccga gaaacttgta aaaggtttgc gcctgcctaa atccctgccg
     1141 gtggtcaccg gcgacgatgt caaaaacgcc aagcccgctc cggacatttt cctgttcgct
     1201 gccgaaaagc tgggtgtcgc aatcagtgat tgcatcatcg tcggcgatag cccttgggac
     1261 ttgctggccg ggcgccgcat gaaagcgctc ggcgtcggtt tattgtgcgg cggctacgcc
     1321 aaagaagagc tcgagcgcgc cggtgcgcac ctcgtatacg acgatcccgc cgaactcctc
     1381 aaaaatttaa atgaattggg gatcaatgaa gagaagtaac aagcaggtca gaactcaacc
     1441 actctgccgg gggaatcatg ccatctaaac aaagcgccgc agcacgcaaa aacatcaaga
     1501 aagccgccaa cgccgccaga aaaaagcgca ccatcgcgca tcttccaaag tctgtgcgca
     1561 ccgcactagg caaaaaaggc gcgcaagcag caaaacgcaa ccgctcaaag cgctgacccg
     1621 ttctgttttt tctcagcgcc aaataagt
//
LOCUS       DATCBB010000455         1024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_404759, whole genome shotgun sequence.
ACCESSION   DATCBB010000455 DATCBB010000000
VERSION     DATCBB010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404759"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            574..969
                     /locus_tag="VII37_05050"
     CDS             574..969
                     /locus_tag="VII37_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069127.1"
                     /translation="MGWKQERDLLIAQTMAFVQSVTGKKPDAGLAEATPGIGATPAAK
                     TAPVSAVEMLAAEIIAPPQAGPQVSPQAHIRIPRSNVSSDIRSEMQARVANFRAHQER
                     FNREREAYCSATLTKLRAAIDNAPTPGRP"
BASE COUNT          220 a          315 c          285 g          204 t
ORIGIN      
        1 aaaaatttcc agttgcgatc gaggaaacgg cgcgcgcgcg tcatccagtt ggctttctta
       61 aacatcaggc aagcctcatc gttgcgcgaa acgacatcgc gcgaggtgat tgacagattg
      121 cctggagaga gggagaaccc tcatgttgcg gagagagtat cgactcaatc caggcgccag
      181 gtggtgtttt ggaagaacac tgcctggctc acctattgga tgtgattgat ttgcggattc
      241 cgcgcaagcg ccatttgcgg ctgtggatat ccgacctttt attccgattc ctgaaattaa
      301 atcaaccgct taacctgtgg acggcgaccc aatccgcaaa acttaaatgt ggatactacc
      361 tgctattcac ggagcgaaat gaatttcgtt ctagcaatcg cgcattagag caccgtccac
      421 aattcttcaa cgggcatccc gtgaagcatt gggcacgctg taaccctttt accacgcccc
      481 gcatcaatct gatcgccgcc tgcccaaacc ccgcatcccg tccttgcttc cccacgcgcc
      541 ctttgctgga atattttcgg gctgaggtgc gtgatgggtt ggaagcaaga acgcgatctc
      601 ctgatcgcgc agacgatggc gtttgtgcag tcggtgaccg gcaagaagcc ggacgccggg
      661 ctggccgagg cgacgcccgg cattggggcg acccctgcgg ccaaaacagc gccggtttct
      721 gccgtggaaa tgctggctgc ggagattatc gcgccgcccc aggccggccc gcaagttagc
      781 ccccaggccc acatccgaat cccgcgatca aacgtctcga gcgacattcg cagcgagatg
      841 caggcccgtg tcgccaattt ccgtgcgcat caggagcgtt ttaaccggga gcgcgaggca
      901 tattgcagcg ccaccctgac aaaactgcgc gcggcgatcg ataacgcccc cacgcccggc
      961 cgcccctgag gcactgtgcg agcgcgggtc ctaacccctg atcttcgcca gcaccggcag
     1021 caga
//
LOCUS       DATCBB010000456         1279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_79689, whole genome shotgun sequence.
ACCESSION   DATCBB010000456 DATCBB010000000
VERSION     DATCBB010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79689"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1168
                     /locus_tag="VII37_05055"
     CDS             287..1168
                     /locus_tag="VII37_05055"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5069128.1"
                     /translation="MNSVGPQTRVGLIGLGLMGKPMGMNLLKAGFPLTVWNRTASRAD
                     ELVAAGAKLARSPRELAANCEFVISIVSDPTALEEVLWGANGAMDGLQRGSIYMDSST
                     VSPTLARKLAAACEERGVRFLDAPVTGGDWGAKKGELVFMVGGDAATLAEVEPVLRVM
                     GKKWFHLGANGAGQTIKLAMNLILALQVDALAEALALVTAAGLQGEKLVEVMQSSMAR
                     SGVLDIKSQNLLKGDYTPSFPLRLMHKDMRLALELAQQTHVELPATTAAFKTYSSVKE
                     AAKEDLDYSAVMKFWKK"
BASE COUNT          289 a          314 c          403 g          273 t
ORIGIN      
        1 tcgcttcgct ctgaaccccc cgcggcggag accgagcagc ccagcgatgc cagcgaggaa
       61 gccaaaagca cagcgcacga aaaatcgacc gttaatccct tgagcgttat tttcccgcgg
      121 ccgcagggat ccaacattta actttcgcct gagtttcagc tactcattgc gccctcgacg
      181 tttcagactg catcctcacg cgcttcgaaa aattgactac gctacaattg agttcgacga
      241 ctgaacttaa tcactggaga tgcttttcga tcgagcggag ggttttgtga attccgtagg
      301 accgcaaacg agggtgggac tgatcggtct ggggttgatg ggcaagccca tgggcatgaa
      361 tttgttgaaa gccggctttc cgttgacggt ttggaaccgc acggcgtcac gtgccgatga
      421 actggttgcg gcaggagcga aacttgcacg atcgccgcgg gaactggccg ctaattgtga
      481 atttgtgatt tccattgtca gcgacccgac agcgctggaa gaagtgttgt ggggagcgaa
      541 cggtgcgatg gacggtttgc agcgcggcag catttacatg gactccagca ccgtatcgcc
      601 gacccttgcg cggaagttag cagcggcttg cgaggagcga ggagttcgct tcctggacgc
      661 accggtaaca ggaggcgact ggggcgcaaa aaaaggcgaa cttgttttca tggttggggg
      721 cgatgccgcg acgctggccg aagtcgagcc agtgttgcgc gtgatgggga agaaatggtt
      781 tcatctcggg gcaaacggag cgggacaaac gattaagctg gccatgaacc tgattctggc
      841 gttgcaagtg gatgcgttgg cggaagctct tgcgctggtg acggccgcgg gtttgcaagg
      901 agaaaagtta gtggaggtca tgcagtccag catggcgcga tcgggtgtgc tggacatcaa
      961 gtcgcaaaac ttgttgaagg gcgattacac gccaagtttt ccgctgcgat tgatgcacaa
     1021 agatatgcgg ctcgcgcttg agttggcgca gcagactcac gtggagcttc ccgctaccac
     1081 tgccgcattt aaaacgtact ccagcgtgaa agaagcggct aaggaagacc tggactattc
     1141 cgcagtgatg aaattctgga agaagtaagt tgagcgccga gttttgccgt tacgttcgca
     1201 aacggtttcc ttacgccgca attatggccg ggcgtttggc gagaagaagc caatcggcag
     1261 gtcccgagcg gtgcgcaat
//
LOCUS       DATCBB010000457         1019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_274945, whole genome shotgun sequence.
ACCESSION   DATCBB010000457 DATCBB010000000
VERSION     DATCBB010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274945"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VII37_05060"
     CDS             complement(<1..418)
                     /locus_tag="VII37_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY5069129.1"
                     /translation="MRQPAKVTIRKLPTGVRGLDDILGGGIPEFSFNIIAGTPGCGKT
                     TMAHQIVFANATAKKPALFFTVLGEPAIKMLRYQQQFTFFDESKVGKAVRFINLSDLV
                     LNQDLNVVLAEIIQQVTAVNPGIVVVDSFRTVVRTAT"
     gene            complement(450..1001)
                     /locus_tag="VII37_05065"
     CDS             complement(450..1001)
                     /locus_tag="VII37_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069130.1"
                     /translation="MEQWTCRIRGAVRVKLPSIPLGQPGAAPCEFVFEMSQATAMLRD
                     FAERLIAFETTENLSFETKQLAGFLVVEKLRLQLATVMGSVGIRALLSRALALATAEV
                     PWLGAVHVRADGSLEGLDKLETELGPKKLAEGGAVLLVQLLGLLVAFIGERLTLQLIR
                     ESWPNFSLSDLNFASGDMHDNPK"
BASE COUNT          214 a          295 c          297 g          213 t
ORIGIN      
        1 ccgtcgctgt gcgcaccacg gtgcgaaagg agtccaccac cacgatgccc ggattgaccg
       61 ccgtgacctg ctggataatt tcagcgagga ccacgttcaa atcctgattc aacaccaggt
      121 cgctcaggtt gataaagcgg acggccttgc cgaccttgga ttcgtcgaag aaagtaaact
      181 gttgctggta gcgcaacatt tttatggcgg gttcgccaag gacggtgaaa aagagcgcgg
      241 gtttcttcgc ggtcgcattg gcgaaaacga tctgatgcgc catcgtggtt ttgccgcagc
      301 ccggcgtgcc cgcgatgatg ttgaaggaaa attcgggaat gccgccgccc aatatgtcgt
      361 ccaggccgcg cacgccggtc ggcagcttgc ggatcgtgac tttggccggt tggcgcatgc
      421 tcgttttggc gggtttgcgt ttctccgcgt cattttggat tgtcatgcat atctccactg
      481 gcgaagttca gatcagagag ggaaaaattc ggccatgatt ctcgtatcag ttgcagcgtc
      541 aggcgttcgc cgataaaggc caccagcagt ccaagcagtt ggacgagcag aaccgctccg
      601 ccttcggcga gcttctttgg cccaagctcg gtttcaagct tgtccagccc ttccagggag
      661 ccatccgccc ggacatgcac cgcgcccagc catggaactt ccgcggtggc cagtgcgagc
      721 gcgcgggaaa gcagcgcccg aataccgacg ctgcccatga ctgttgcaag ctgcaggcgc
      781 agtttctcaa cgaccagaaa acccgccaac tgtttcgtct caaaagaaag attttccgtc
      841 gtctcaaagg cgatgagacg ctcggcgaaa tcgcgcaaca ttgcagtggc ttggctcatt
      901 tcgaaaacga actcgcatgg ggcagcgccc ggttggccca gcggaatgga gggcagtttg
      961 acgcggaccg ccccccttat tcggcaggtc cactgctcca aattccggag tttcaagca
//
LOCUS       DATCBB010000458         1163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_177551, whole genome shotgun sequence.
ACCESSION   DATCBB010000458 DATCBB010000000
VERSION     DATCBB010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177551"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VII37_05070"
     CDS             complement(<1..463)
                     /locus_tag="VII37_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069131.1"
                     /translation="MYRFLSGGTKTLMAAFVALALLALCVWWLGFRGSPAQSARDFEE
                     CVEQDQAKGPSNDQRGALTTGCNARFAGRRKAGGGYTYYDFMQNRNFDIAGPNPTAEE
                     RKQIDREYMGFLDAQRREAMSAELARKQNEQLQAEIESARQPVGPPMILLPR"
     gene            956..>1163
                     /locus_tag="VII37_05075"
     CDS             956..>1163
                     /locus_tag="VII37_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069132.1"
                     /translation="MFLFAALMLANCCALGNGCAPASGVPPTAWDGLGSAPSDDTQPV
                     EPPPGKHARANREIIAGPLDAAAAE"
BASE COUNT          233 a          330 c          334 g          266 t
ORIGIN      
        1 tcctcggcag caatatcatc ggtggcccga cgggttgacg cgcgctttct atttccgcct
       61 gaagctgttc gttctgtttt cttgccagtt cagccgacat cgcctctcgc cgttgggcat
      121 cgaggaagcc catatactcg cgatcgatct gcttgcgctc ttccgctgtc gggttggggc
      181 ccgcaatgtc gaaattccgg ttctgcatga agtcgtaata agtatagcct ccgccggctt
      241 tgcgccggcc tgcgaaccgc gcgttacagc ccgtcgtcaa agcaccgcgt tggtcgttcg
      301 atgggccttt ggcctggtcc tgctcgacgc actcttcaaa atcccgggca gactgagctg
      361 gcgacccacg aaaacccagc caccagacgc agagcgcgag caaagccagc gcaacgaatg
      421 cggccataag cgttttggtg ccaccgctca gaaaacggta catgcgctag aatcccccac
      481 aagttgtaca ctagtgcagc aaatgcgggt ttttgcgggc aagcaccctt cgatgtagca
      541 acgcagccac agtcagccgg attttttgtc gagcaagaat ctcgccacga ttgagcacag
      601 attgtcgaac agagttggat ctgtcgctta gtaaagggtt ctggtcatgc gatcagtaaa
      661 agctattgca acattggcgt cggcagccgc gattgtttgt tcaatatttg catttagctg
      721 ggtcagcctt gcgctgctcg gattttgagt gtggcattga accagcgcgg tgccggcgct
      781 agtggagcgt tttcaagcga agcgtagacg cggttcgcgt cgaagaaacg cgtcaaatca
      841 ggtatccgga gcccgtttcg attccaccga aacgggaagg ctttggcact agatcgccat
      901 tttcagtctt ttattgcaac gctgtaaagt gatcccgatg attgcgaaat gctctttgtt
      961 cctgtttgcc gcattgatgc tcgcgaattg ttgcgcgttg ggaaatggtt gtgcgccggc
     1021 gtccggagtg ccgcccaccg cgtgggacgg actcggctct gctccgagcg acgacacaca
     1081 gccggttgag cctccgcccg ggaagcatgc gcgggcaaac agagaaatca tcgccggccc
     1141 gctcgacgct gcggcggccg aac
//
LOCUS       DATCBB010000459         1977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_404869, whole genome shotgun sequence.
ACCESSION   DATCBB010000459 DATCBB010000000
VERSION     DATCBB010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404869"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1953
                     /locus_tag="VII37_05080"
     CDS             229..1953
                     /locus_tag="VII37_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY5069133.1"
                     /translation="MTETSPVVAKKSGDESYRDVSLRNFSLRDLSLSEVSWAVLILLT
                     LYVCYFSHLGAFGLIGPDEPRYAWIARDMAESGDWVTPRLYGKPWFEKPVLYYWAAAV
                     CFKLFGENEASARLPSAVCALLATLALAWLALRLGGWEMARWVLLLLPSTVGMIGFSH
                     AAATDMPFSAMLSVAMVCAAVALNLVPRNFSEKNPPDEVSADETREDMLAYRQTSKTS
                     YLSLALFGFFLGLAVLAKGPAALILAGGAVFLWSALTKRWRDLLRLFHPIAIGTFLLT
                     SLPWYVLCALRNPDFFRIFIIEHNFKRYLTPEFQHIQPFWYYLPITILALLPWLFWLA
                     WFVKRENRGDEGDGYQRTQVLFLAAWGIFPILFFSLSRSKLPGYILPAVLPLGLLISF
                     AVARALKSGHAFGRYVLGLIGLLLFASPGWIIFSKAHVRGPLVSLYIIVAAAGGLIVI
                     GAAVLRHTRAGLTFSAVLVLFLLNLVYVSLGKLDPRLSARSCARQIGAERAAHTYSFK
                     LQRSWQYQLNFYLHHEIMEWSPRVAGEATVVTNEKNLEELERSAQIVAVISKQSPEAE
                     VVVVRPLF"
BASE COUNT          369 a          483 c          572 g          553 t
ORIGIN      
        1 gaaggaaaag catatgcgcg acggaagcgg ccagcgaccg cgctatctgg cgtggcaaca
       61 cttccagaat ttttaatgac acgcgtacag ccgcgtactc tgcccattcc ttcatcgatt
      121 atttgagtgg ctgccctccg ctgcctcgac agtatgcctt tgggcgcggc ttgattgcca
      181 tggacattgc gcacgatgtt agactcacgt ccgtagttcc ttcttcgaat gaccgaaacc
      241 tcaccagttg tggcgaaaaa atccggcgac gaatcctacc gcgatgtctc tcttcgcaac
      301 ttttccctac gcgacctgtc cttaagtgag gtcagttggg cagtgctcat tctgctgacg
      361 ctgtacgttt gctacttcag tcatctgggg gcttttgggt tgatcgggcc tgatgagccg
      421 cgctacgcgt ggatcgcgcg agatatggcg gaatcgggcg attgggtcac gccgcgactt
      481 tacggaaagc catggttcga gaagccggtc ctttactact gggccgctgc ggtatgtttt
      541 aaattgttcg gggaaaacga agccagcgcg cgattgccga gcgcggtatg cgcactgttg
      601 gccacgcttg cgttggcgtg gttggcactt cgacttggtg gttgggagat ggcgcgctgg
      661 gtgctgttgc ttttgccgtc cacggtggga atgatcggtt tttcacatgc agccgcgacg
      721 gacatgccat tcagcgcgat gttaagcgtt gccatggttt gcgcggctgt tgcgctgaat
      781 ctggtacctc ggaatttttc agagaaaaat ccgccggacg aagtatctgc cgatgaaaca
      841 cgtgaagata tgttggccta tcggcagacg tcgaaaacta gctatctcag tttggcgctt
      901 tttggatttt tcctggggct tgccgttttg gccaagggtc cggcggcgct aatactcgcc
      961 gggggcgcgg tgtttttgtg gtcggctttg acgaaacgtt ggcgcgattt gcttcgattg
     1021 tttcacccca tcgctatcgg cacattttta ctgacatcgc ttccctggta tgtactgtgc
     1081 gctcttcgca atccagattt tttccgcatt tttatcattg aacacaactt caagcgttat
     1141 ctgacgccgg aatttcagca cattcaaccg ttttggtatt acctgccgat tacaattctg
     1201 gcgcttctgc cgtggttatt ttggctggca tggtttgtta agcgcgaaaa caggggagac
     1261 gaaggtgatg gctaccagcg aacacaagta ttgtttttgg cggcgtgggg aatctttccc
     1321 attttgtttt tttcgttatc gagatccaaa ctgcccggct acattcttcc ggcggtactt
     1381 ccattggggc ttctaatttc attcgcagtc gcgcgggcgt tgaaatccgg acatgcgttc
     1441 gggaggtacg ttctcggact gattggcttg ctgttattcg cgtcgccggg ttggataatt
     1501 ttttctaagg ctcatgttcg aggaccgctg gtatcgctct acattattgt ggcggcagca
     1561 ggtggtctaa ttgtgattgg ggcggcagtg ctacggcata cgcgtgccgg tttaactttt
     1621 tctgctgtgc tggtgctctt cctgctgaat ttggtgtatg tgtcgctggg taagctggat
     1681 ccccgactct ccgcgcggag ctgcgcgagg cagatcggtg cggagcgcgc agctcatact
     1741 tattccttta aactgcaacg ctcgtggcag taccaactga atttttatct gcaccacgaa
     1801 attatggaat ggagtccccg cgttgccggc gaggccacgg tggtgaccaa cgagaaaaat
     1861 ctggaggaac tcgagagaag cgcgcaaatt gttgcagtaa tttccaagca atcgccggag
     1921 gcagaggttg tggtggtgag gcctttattt taagggccgc tgctttttac tgggccg
//
LOCUS       DATCBB010000460         1833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_371724, whole genome shotgun sequence.
ACCESSION   DATCBB010000460 DATCBB010000000
VERSION     DATCBB010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371724"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1048)
                     /locus_tag="VII37_05085"
     CDS             complement(146..1048)
                     /locus_tag="VII37_05085"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5069134.1"
                     /translation="MLDLAGVYPALTTPFAADGSVSLEGMKHNIGRYNHTALAGYVVL
                     GSTGESVLLLSNEMDGILGAVKETAAPGKKLIAGTGAESTAETIERTQRAAALGYDVA
                     LVKTPYYYKPAYKPEVLIAHYRRVADESPIPVLLYSVPIFTGLALEAPETIALAEHKN
                     IIGIKDSSGNVQRISEMIAGAPAQFQVLVGAAATVFPCLAVGARGAILALASALPEKC
                     ATLYELFRHGQMDQARELQKVLLGASNQIVSKFGIAGVKYVMDQRGYRGGNPRLPLQP
                     LTEEHKARISSFLATIEQPIMASA"
     gene            complement(1101..>1833)
                     /locus_tag="VII37_05090"
     CDS             complement(1101..>1833)
                     /locus_tag="VII37_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069135.1"
                     /translation="LFVSKATGVPIAKVAARLMAGKKLSTMKLPLVVHHGVSEIALHE
                     FYSVKSPVFPFNKFRGVDTILGPEMRSTGEVMGISTSYGLAFAKAQLAAGQRLPRKGT
                     VFMSVNDRDKKHVGPLGKELQALGFKLLATRGTAAALADSEVEAQEVFKVNEGRPNIV
                     DLVKTGKIDLIINTPLGRESFYDEKSIRRAAIRYNIPCITTLAAAHAAARGIQALLEQ
                     GTEVAALQDLHRGSVAASKSPTSAD"
BASE COUNT          403 a          552 c          467 g          411 t
ORIGIN      
        1 tttcaccttc gaaaaattat tcagatattc gaggaacagc ttcgactggt ggggtaactt
       61 gtgggacgcg agggcacgac agtccatgag ccgttagatg atacccgtgc ggaaacgtct
      121 catcaaactg ccgaattatt acaactcatg ccgaagccat aatgggctgc tcaatagtgg
      181 ctaaaaatga gctaatccgc gccttatgct cctcggtgag cggctgcagc ggcaacctgg
      241 gattccctcc gcgataaccc cgctggtcca taacgtactt aacgcccgca atgccgaact
      301 tcgagacgat ctggttggag gcgcctaaca gcaccttctg caattcgcgg gcttggtcca
      361 tctggccgtg tcgaaaaagc tcataaagcg tcgcgcattt ctctggaagc gcgcttgcca
      421 acgccagaat cgctccccgc gcacccaccg caaggcatgg aaacactgtg gcggcagcac
      481 ctaccagcac ctgaaattgt gcaggcgcac ccgcgatcat ctccgaaatg cgttgaacat
      541 tcccagagct gtcctttatg ccgatgatat ttttatgttc ggcgagcgcg atcgtctcgg
      601 gtgcttcgag cgccagtccg gtaaagatgg gtacggaata aagtagtacc gggatcggcg
      661 attcatcggc gacgcggcgg taatgcgcga tgagcacttc cggtttataa gcaggcttgt
      721 agtagtaagg agttttcacc agtgcgacgt catatcccag ggccgccgct ctctgcgttc
      781 gttcaatggt ttccgccgtg gattccgcgc cggtgcccgc gataagtttt ttcccgggcg
      841 ccgctgtctc tttcaccgcg ccaagtatgc cgtccatctc gttggacagc agcagtaccg
      901 attctcccgt ggaaccgagc accacatacc ctgcgagtgc cgtgtggttg tagcggccga
      961 tgttgtgttt cataccctcg agcgagaccg atccatccgc cgcgaacggt gtcgttaaag
     1021 cgggataaac tcctgccaga tccagcattg aattccccat atcagttgca aaatcagctt
     1081 acaaaatata gtcggcgccg tcagtccgcg gaagttggac ttttcgaagc cgccacgctg
     1141 ccccgatgca aatcctgcag cgccgctact tcggtaccct gctcaagcaa cgcctgtatc
     1201 ccgcgagccg ccgcgtgtgc cgctgccagc gttgtgatac agggaatgtt atagcgaatc
     1261 gccgcgcgcc gaatagattt ttcgtcgtaa aaagactcgc gccccaatgg agtattgata
     1321 atcagatcga tcttcccggt cttcaccagg tccacgatgt tgggccgccc ctcgttcacc
     1381 ttaaaaactt cctgcgcttc cacttccgaa tcggccaacg ccgcagccgt tccgcgcgtg
     1441 gccaaaagtt tgaaacccag cgcctgtaat tccttgccca gcggcccaac atgctttttg
     1501 tcacggtcgt taacgctcat aaacacagtg cctttgcgcg gcaaccgttg cccggccgcg
     1561 agttgtgctt tggcgaatgc cagcccgtac gaagtcgaaa tccccatcac ctcgccggtg
     1621 gaacgcattt ccggtccgag aatggtgtcc acaccgcgaa acttgttgaa cgggaaaacc
     1681 ggcgacttca ccgaataaaa ttcatgcagc gcgatttctg aaacaccgtg atgaacaacc
     1741 agcggcaact tcattgtgga caattttttg cccgccatca atcgtgccgc caccttagct
     1801 attggcactc cagtcgcctt gctcacaaac agc
//
LOCUS       DATCBB010000461         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_79779, whole genome shotgun sequence.
ACCESSION   DATCBB010000461 DATCBB010000000
VERSION     DATCBB010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79779"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VII37_05095"
     CDS             complement(<1..749)
                     /locus_tag="VII37_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5069136.1"
                     /translation="MAEPAIDIENLTKDYPYGFLQLKKRRSLEGLTMQVQPGEVFGFL
                     GPNGAGKSTTIKLLMRLIFPTAGSARILGKPISDISMHRDIGYLPEQPYFYDYLTAEE
                     VLDYFARFHDFGATERRERVQRMLKRVGLESAGRIQLRKYSKGMLQRVGLAQAILHDP
                     AVVVLDEPMSGLDPVGRREVRDIILELRKQGKTVLFSTHILSDAEMLCDRVGVIVGGK
                     LQGVGAPSELVGLKSQGMEILFELPAGAPSGG"
     gene            970..1278
                     /locus_tag="VII37_05100"
     CDS             970..1278
                     /locus_tag="VII37_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069137.1"
                     /translation="MAKQNVDNLNVTCPCCHSKLVVDSVFGAVLSHEAPPKTGPNVDL
                     TDAAGILNEQQRNREDKFRDSWNLEHNKEDILAKKFEEAMKKAKDAPAGKPIRDIDLD
                     "
     gene            1354..2238
                     /gene="folP"
                     /locus_tag="VII37_05105"
     CDS             1354..2238
                     /gene="folP"
                     /locus_tag="VII37_05105"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5069138.1"
                     /translation="MIPRRKFRLKLRSRTLILGPRTLVMGVLNVTPDSFSDGGNFFSQ
                     EKAVQHALQMERDGADLIDIGAESTRPGSVGITAEEEWSRLAPVLAALRTRLKIPISV
                     DTRKSEIATKAIESGAELINDVSGLKHDPRIAQISARARVPLILMHMRGEPETMQQKP
                     FARDALKDVTQGLRASLAIARKAGVANSQIVLDPGIGFGKSYAQNYELLADLPQLAKL
                     GFPLLVGTSRKGFISKTLAPDGKPAPPETKIWGTAATVTASILNGAHIVRVHDVAQMV
                     SVARIADCILNPSLAPKN"
BASE COUNT          617 a          800 c          537 g          490 t
ORIGIN      
        1 cctccgctcg gcgcgcccgc cggcaactca aagagaatct ccatcccttg cgatttcaat
       61 cccaccagtt cactcggcgc tccaactcct tgcaacttcc ctccaacaat cacgcccacc
      121 cgatcgcaca acatttccgc atcggacaaa atgtgcgtgg aaaataaaac cgttttcccc
      181 tgttttctta gttccagaat gatgtcgcgc acttcccgcc gccccaccgg gtccagtccg
      241 gacattggct catccagcac caccaccgcc ggatcatgca aaatcgcctg cgccagcccc
      301 acccgctgca gcattccctt cgaatatttt ctcaactgaa tgcgcccggc cgactccaat
      361 ccaactctct tgagcatgcg ctgcacacgc tcgcgcctct ctgtcgcacc gaaatcatga
      421 aagcgcgcaa aataatccag cacttcctcc gcggtaaggt aatcgtagaa atacggttgc
      481 tccggcaagt agccgatatc ccggtgcatg gaaatatcgc tgatcggctt cccgagaatc
      541 cgcgccgatc ccgccgtggg aaaaatcaac ctcatcaaca gcttgatcgt cgtcgactta
      601 cccgcgccat ttggccccaa aaaaccaaaa acttcgcccg gctggacctg catggtcagg
      661 ccttccagcg agcgcctttt cttcagttga agaaagccgt acgggtaatc cttggtgaga
      721 ttctcaatat caattgccgg ctcagccata tattcattct tgccatacgc cccaaaagcg
      781 tcaatcgtgc gccacccagc ctgtgcacgg atagaaaata ccgcatagac acaggctgga
      841 aatgattgtt cacaaagcga ggtttcttgt acaaaaatgg ccgaattatc ggcccgtcaa
      901 gcccaatcca ggtgcggtac cgtcatcgct acttctacgt gcccggcaaa aaggcgtaga
      961 atactttcca tggccaagca aaacgtagac aacctgaatg ttacttgccc atgctgccat
     1021 tcgaaactgg tggtcgattc tgtttttgga gccgtacttt cccacgaagc gccacctaaa
     1081 acaggtccaa acgtcgacct caccgatgcc gcgggcatct tgaatgagca acaacgcaac
     1141 cgcgaagaca aattccgcga ctcctggaac ctcgaacaca acaaagaaga catcctcgcc
     1201 aaaaaattcg aagaagccat gaagaaagcc aaggacgctc cggcaggaaa gccgattcgc
     1261 gatatcgatt tggattaatt atcctgaacc gcgccgtccc tttccacact cacctgagca
     1321 ccatcagttg cctttgcatt acactgccat gaaatgattc cgcgccgtaa attccgcctg
     1381 aaactgcgct cccgcaccct tattctcggc ccacgcaccc tggtgatggg cgtcctgaac
     1441 gtaacccccg acagtttctc cgatggcggc aatttttttt cacaggaaaa agccgtgcaa
     1501 cacgctttgc aaatggagcg cgacggtgcg gacctaatcg acatcggcgc tgaatccacg
     1561 cgcccaggct ctgtgggcat caccgccgaa gaagaatgga gccgccttgc gcccgtgttg
     1621 gccgccttac gcacgcgctt aaaaattccc atctccgtgg acacgcgcaa gagtgaaatt
     1681 gccaccaaag ccatcgaaag cggcgcggag ctaattaacg atgtgagcgg cctgaaacac
     1741 gatccccgta tcgcccagat ttctgcccgc gcgcgcgtcc ctctgatcct catgcacatg
     1801 cgcggagaac cagaaaccat gcagcaaaag cctttcgcgc gcgacgcact gaaagacgta
     1861 acccaaggcc tgcgcgcatc cctggccatt gcccgcaaag ccggcgtcgc taattcccag
     1921 atcgtcctcg atcccggcat cggcttcgga aaaagctacg cccaaaatta cgaattactc
     1981 gccgatctgc cgcaactggc aaaactcgga ttccctctac tcgtcggcac ttcacgcaaa
     2041 ggttttatca gcaaaacact agcgccagac ggcaagccgg cgcccccaga aacaaaaatc
     2101 tggggcaccg ccgccactgt cacggccagc atcctgaacg gtgcacacat cgtccgcgtc
     2161 cacgacgtag cccaaatggt aagtgtcgcc cgcatcgccg actgcatcct gaatcccagt
     2221 ctggccccca aaaattagtc tccggtcgaa atcgccgccg ctcggattaa agtcaatcag
     2281 aattcactca tcacaaatcc aaaaataacg gagggtgccc caggttcgca gttgaggtat
     2341 gaacctgggt cttgtcgtct ccgaatattt ccaaatatca aatcgacagc tacgaagcac
     2401 acatcattag ccctccgtct cactctttcg gtataatccg ccgc
//
LOCUS       DATCBB010000462         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_371768, whole genome shotgun sequence.
ACCESSION   DATCBB010000462 DATCBB010000000
VERSION     DATCBB010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371768"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VII37_05110"
     CDS             <1..737
                     /locus_tag="VII37_05110"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY5069139.1"
                     /translation="AGMTVHAGAGNATGTMVNALLGRGQSLSQLGDALRPGIVHRLDK
                     ETSGVILVAKTDAAHARLGEAFRQRNVNKTYIALVQGSFETTSGRIELAIGRDPIKRV
                     RMAAEQKIWRGANSSSKIANSREARTDWRTLLPIDATTLLEVQLHTGRTHQIRVHFSA
                     LKHPVVGDTLYGAAAQLRVGDVVLPPLGRNFLHAAKIVFSHPRTGERMELRAPLPVEL
                     RDFLHTLAKTAGGDPGKIDAALHGYL"
     gene            747..1856
                     /locus_tag="VII37_05115"
     CDS             747..1856
                     /locus_tag="VII37_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5069140.1"
                     /translation="MQRKPILILALAVGAIFLLPAQHSAQQPPSPAPPAQSTPPATAP
                     DSSQAPPAGQNPTVIQSRVNLVDILFTVLNRRNKLVPDLEKGDFKIFDDKAPQEIRYF
                     SKQTDLPLRIGMLLDTSNSIRDRLKFEQDASTNFLFSVLRHNKDEAFVMTFDDEPQVI
                     QKFTGDAGALRDEIFKTRAGGGTAIYDAIYSACLNELSHPPRPPGDLPDVVRRVMILI
                     SDGEDNLSNRTRAEAIEMAQRASVVIYTISTSTQWIALSQTDPSKAANRKNHLTSGDQ
                     ILQDLAEETGGRAFFPYHVDDLDQSFQDIGDELRNQYSIAYSPTNYVMDGKYHKIRIE
                     TPDHKGYQVRARHGYFARANATSPAAGTPPAGSPR"
     gene            complement(1790..>2288)
                     /locus_tag="VII37_05120"
     CDS             complement(1790..>2288)
                     /locus_tag="VII37_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5069141.1"
                     /translation="AIYLASGEKLNTEAGRKAIVIVTDAQDEGSKVRLEEAIESAQRT
                     DSVIHVLLVADRRFGGGNGGVAKKLADETGGRMIVVNSEKKLKEAFDEISDELRSQYT
                     LGYYPSNAARDGKFRKIKVETNNHDLKVLARKGYYAPKGEAAYRGEPAGGVPAAGEVA
                     LARAK"
BASE COUNT          502 a          781 c          528 g          477 t
ORIGIN      
        1 ccgccggcat gaccgtgcac gctggagcag gtaacgcgac cggcaccatg gtcaacgcgc
       61 tgctaggacg cggccagtcg ctctctcaac taggcgacgc gctgcgcccg ggtatcgtgc
      121 accgcctcga caaggaaacc tctggggtca ttttagtcgc caaaaccgac gcggcccacg
      181 cccgcctcgg cgaagccttt cgccagcgca acgtaaataa aacctatatt gccttggttc
      241 aaggctcctt cgagaccact agcggacgaa tcgagttggc cattggccgc gaccccatca
      301 agcgcgtacg tatggccgca gaacagaaaa tctggcgcgg cgccaactcc tcctctaaaa
      361 ttgccaactc ccgcgaagca cgcaccgact ggcgcaccct gcttcctatc gacgccacta
      421 ctttgctcga ggtccagctt cataccgggc gcacccacca gatccgcgtt catttttccg
      481 cgctgaaaca tcccgtggtg ggcgacaccc tgtacggagc cgccgcccag cttcgcgtgg
      541 gcgacgtagt cctcccgccg ctagggcgca atttcttgca tgccgctaaa attgttttct
      601 ctcacccgcg aactggcgaa agaatggagc ttcgcgctcc attgccggtg gaactccgcg
      661 actttctcca cacgctagcc aagaccgccg gcggagaccc aggaaaaatt gacgctgcgc
      721 tccacggata cctataatct gggcagatgc aacgcaaacc aattttgatt ttggcactcg
      781 ccgttggagc catattcctt ttgccggcgc agcattccgc gcaacaaccc cctagccccg
      841 ctccgccggc gcagtccacg cctcccgcaa ctgctcccga ttcgtcacaa gcgccacccg
      901 cgggccagaa ccccaccgta attcagagcc gcgtcaatct tgtggacatt ttgttcaccg
      961 ttttaaatcg ccgcaacaaa ctcgtccccg acctggaaaa aggcgacttc aagatttttg
     1021 atgataaagc cccgcaggaa attcgctact ttagcaagca gaccgatctt cctctgcgca
     1081 ttggaatgtt gctcgacacc tccaacagca ttcgcgaccg cctgaaattc gaacaggacg
     1141 cctccacaaa tttcctcttc agcgttttgc gtcataacaa agacgaagct tttgtcatga
     1201 cctttgacga cgagccgcaa gtaatccaaa aattcaccgg cgacgcggga gccctacgtg
     1261 acgaaatttt caagactcgc gcgggcggcg gtacggcaat ttacgatgcc atctactccg
     1321 catgcctgaa tgagttgagc cacccgcccc gtccaccagg cgacctgcct gacgtggtac
     1381 gccgcgtaat gattttgatc agcgacggtg aagacaatct ctccaaccgc acccgcgccg
     1441 aagccatcga aatggctcag cgcgctagcg tagtgattta caccatcagc accagcacgc
     1501 aatggatcgc gctttcgcaa accgatcctt ccaaggccgc caatcgcaag aaccatctca
     1561 ctagcggcga tcaaattttg caggacttag cggaagaaac cggcggccgt gctttctttc
     1621 cctaccacgt ggatgattta gatcaatcgt tccaggacat cggagacgag ctgcgcaatc
     1681 aatactccat cgcttacagt cccaccaact acgttatgga tggcaagtac cacaaaatcc
     1741 gcatcgaaac ccccgatcat aagggctacc aggtccgcgc caggcacggt tacttcgctc
     1801 gtgccaacgc cacttcacca gcagccggaa cgccgccagc cggctcaccc cggtaagcgg
     1861 cttctccctt cggagcgtag tagcctttcc gcgccagcac tttcaagtcg tgattattcg
     1921 tctcaacttt aatcttgcga aacttcccat ctcgcgccgc gttactcggg taatacccca
     1981 gcgtgtactg gctgcgcagc tcgtccgaaa tttcatcgaa cgcttctttt aatttcttct
     2041 ccgaattcac cacaatcatc cgtccgccgg tttcatccgc cagcttcttc gccactcccc
     2101 cattcccgcc gccaaatctc cggtccgcca ccagcaaaac gtgaatcact gaatccgtcc
     2161 gctgcgccga ctcaatggct tcttcaagcc tcactttgct gccttcgtcc tgcgcgtccg
     2221 tcacgatcac gatggctttg cgccccgcct cggtattcag tttttctccg ctcgccaaat
     2281 agatcgca
//
LOCUS       DATCBB010000463         1349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_113271, whole genome shotgun sequence.
ACCESSION   DATCBB010000463 DATCBB010000000
VERSION     DATCBB010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113271"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..337
                     /locus_tag="VII37_05125"
     CDS             116..337
                     /locus_tag="VII37_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069142.1"
                     /translation="MIYSRTSVAGFEKFSRKRAMVWLGVDLPCELFFTVFRRGFCLIM
                     GLAIWLAVQIEGRKKSRRWPGFGTAIADY"
     gene            400..>1349
                     /locus_tag="VII37_05130"
     CDS             400..>1349
                     /locus_tag="VII37_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5069143.1"
                     /translation="MEASYKILVLDDEADWLELCRDIFAQLPSKPEIRTCTSSPRALG
                     MLDSEPYRLLILDLKMPRMDGLQVLSVVRRRYPELRTVVLSGLEDEELRSRAYALGAD
                     LFWQKNDMQRDSKMFMECLESLLGRDSDGGFRGIQSKGLMDIIQLECLSRSSTVLRIT
                     RGPLVGRLWFQNGELFDAEAEGARGEVAFRRILAWKSGTFENLPAEPGREHTITKPIN
                     ALLLESAQTMDENANPASETQFIDARHRHTVWKLSLLVREGADFVIAVPPEGQGEPEA
                     LGTQTIDLAGWTRHSTEIARRLGDQVEAGPLLHLTARSME"
BASE COUNT          276 a          371 c          401 g          301 t
ORIGIN      
        1 ctcgatggta tcatcggctg catccgtttc attgtcgtcg aagtgacgca acaggcctcg
       61 cagcaacccg gcaaattttg atttcacggc actcatgttt tttaactcgt gatttatgat
      121 ttatagccgg accagcgtgg ccggctttga aaaatttagc agaaagcgcg ccatggtgtg
      181 gcttggggtg gatttgcctt gcgaactttt ttttaccgtg tttcgccgcg gattttgtct
      241 catcatggga ctagccatct ggctggctgt ccaaattgag ggacgcaaaa aatcccggcg
      301 gtggccgggg ttcggaacag cgattgctga ttattaaaag attatatgtt tttgaacttg
      361 aaattaagct ggttcggcgt tagccgttga cccctcgata tggaagcctc atataaaatc
      421 ttggtgttgg acgatgaagc agactggctt gagttgtgcc gcgacatctt cgcgcagctg
      481 ccaagcaagc cggaaatccg cacctgcacc tcgagtccgc gcgccttggg gatgctggat
      541 tccgaaccat accggctgct gatcctcgac ctgaaaatgc cgcgcatgga cgggttgcag
      601 gtgctctcgg tcgtgcgtcg ccgttatcct gaactgcgca cggtcgtttt gagcgggctg
      661 gaggatgagg aattgcgttc ccgcgcctac gccttgggcg cggatctgtt ctggcagaaa
      721 aacgacatgc agcgtgactc caaaatgttc atggaatgcc tggaatcgct tttgggacgc
      781 gactcggacg gtggtttccg cggcatccag agcaaggggc tgatggacat catccagctg
      841 gaatgccttt cccgcagctc gaccgtcttg cggatcacgc gcggcccgct cgtgggcagg
      901 ctgtggtttc agaacggcga attatttgat gccgaggccg agggcgcgcg cggcgaggtg
      961 gccttccgga gaattttggc gtggaagtcc ggcacgtttg aaaatttgcc ggccgagccc
     1021 ggtcgcgaac acaccatcac aaagccgatc aatgccctgc tgctcgaatc cgcgcagaca
     1081 atggatgaaa acgcgaatcc tgccagcgaa acccagttta ttgacgcccg ccaccgccac
     1141 acagtctgga aactttcact gctggtgcgc gaaggtgcgg attttgtcat cgccgtcccg
     1201 cctgaaggcc agggcgaacc ggaagccctc ggcacccaga cgatcgacct ggccggctgg
     1261 acccggcact cgacggaaat cgcccggcgg ctcggcgatc aagtggaagc cgggccgctc
     1321 ttgcacctga ccgcccgcag catggaaaa
//
LOCUS       DATCBB010000464         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_371877, whole genome shotgun sequence.
ACCESSION   DATCBB010000464 DATCBB010000000
VERSION     DATCBB010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371877"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..>2135)
                     /locus_tag="VII37_05135"
     CDS             complement(138..>2135)
                     /locus_tag="VII37_05135"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY5069144.1"
                     /translation="ERAIQYVYERYGKLGAAMTANVITYRGRSAAREIGKAFAFDPEV
                     LNRLTSLVGAWEYKDANDTLERQFQDAGLDLQHPQIRKFCELCLRFQDLPRHLGQHSG
                     GMVVCQGQLDSVVPLEPATMPGRVVVQWDKDDCADMGIVKVDLLGLGMMAVLEDSLRI
                     IRDDYGEEVDLSRLPANDPLVYKTLQKADTVGMFQIESRAQMACLPRLHPVCFYDIVV
                     QVAIIRPGPIVGQMVNPYLKRRQGREPVTCLHPSLEPVLARTLGVPLFQEQLLRMAMI
                     AAGFSGGEAEELRRAFGFKRSEKCMKDVELKLRRGMDKNGISGETQETIIRSITSFAL
                     YGFPESHAASFALIAYASAYLKCHYLAAFTAAMLNNQPMGFYQPPSLVTDAQRHGLRV
                     RPIDITCSNWLCTLEKIDEQFAVRLGLRYVKGLRAEVGEDIVRDRGVRPFTSVDDLKR
                     RVSRIQKGELAVLAEVGALNRLGNQGGSHRRSALWQVERTARAAGPLYELCDDEKMPV
                     GGAEVNCDSEEYARSPLAQMNAEERLVADYRGTGMTVGPHPMAYHRAELRKMGIRSAA
                     ELAHLPNGMQVRIAGGVIARQRPGTAKGFVFLSMEDETGIANAIISPQLFEECHVVVV
                     HQQFLMIEGQLQNQDNVVSVKSQSIQPLTITSAPTTSHDFH"
BASE COUNT          480 a          704 c          469 g          482 t
ORIGIN      
        1 gcggtgccac ggcagcttta cgcggtgccg ccgccgtcgt tttgcgctta gccgcagatt
       61 ttcccttgct tttggatttc acgctggtta gatgacgttt cgtaacattg cggtcttttt
      121 attattgagg ccagctttta gtgaaaatcg tgcgacgtcg tcggcgcgct ggtaatcgtc
      181 aacggctgga tactttgcga tttcaccgaa accacattgt cctggttctg caactgcccc
      241 tcgatcatca aaaattgttg atgcaccacc accacgtggc attcttcaaa caactgcggt
      301 gagataatcg cgttggcaat gcccgtttca tcctccatgc tcaaaaacac gaatcctttg
      361 gcagtccccg ggcgttggcg tgctatcacc ccgccggcaa tgcgcacctg catgccattc
      421 ggcaaatgcg ccaattctgc ggcgctgcgg atccccattt ttcgtagttc agcgcgatga
      481 taagccatgg gatgcgggcc cacagtcatc cccgtgccgc gatagtcggc caccagtcgt
      541 tcttccgcgt tcatctgcgc aagcggcgag cgcgcgtatt cttcggaatc gcaatttacc
      601 tccgcacctc caactggcat tttctcgtca tcgcagagtt catacaaagg acccgcagct
      661 cgcgccgttc tctccacctg ccacaaagca ctgcgacgat gagatccgcc ctggtttccc
      721 agccggttca aagccccgac ttccgcgagc accgccaatt cccccttctg aattcgcgaa
      781 acgcgccgtt tcaaatcatc cacagaagta aatggacgca caccacgatc tcgaacaatg
      841 tcctctccaa cctccgcgcg caagcctttc acataccgca gccccagcct caccgcaaac
      901 tgttcatcga ttttttccag cgtgcaaagc caattcgaac acgtgatatc aatcggtcgc
      961 actcgcaagc cgtgcctttg tgcatccgta accagcgaag gcggctgata aaatcccatc
     1021 ggctgattgt ttagcatcgc cgccgtaaat gccgccaggt aatgacactt caaatacgcg
     1081 ctggcatacg cgatcaaagc aaaactagcc gcatgcgatt cgggaaatcc atacagcgca
     1141 aacgaagtaa tcgaccgaat gatcgtctcc tgcgtttccc cgctgatgcc atttttatcc
     1201 atccctcgtc gcagcttgag ttccacatct ttcatgcatt tttccgagcg cttgaaacca
     1261 aaagcgcgcc gcaactcttc cgcttcgccg ccgctgaatc ccgccgcaat catggccatg
     1321 cgcagcaatt gctcctgaaa aagcggcacc cccagcgtgc gcgccagcac cggctccagc
     1381 gaaggatgca aacacgtcac cggctcacgc ccttgccgcc gctttaaata aggattcacc
     1441 atttgcccca caatcggtcc cggccgaata atcgccactt gcacaacaat gtcgtagaaa
     1501 cacaccggat gtagccgggg caaacacgcc atctgcgcgc ggctttcaat ttgaaacatc
     1561 cccacggtgt ccgctttttg caaagtttta taaaccagcg gatcgttggc cggcaatcgg
     1621 ctcaaatcca cttcttcgcc ataatcatcg cgaataattc tcagcgaatc ctccagcacc
     1681 gccatcatcc ctagccccag cagatccact ttcacgatgc ccatgtccgc gcaatcgtcc
     1741 ttatcccatt gcaccactac gcggccgggc atcgtcgcag gctctaacgg caccacagaa
     1801 tccagttgcc cctggcaaac caccattcct cccgaatgtt gcccaaggtg ccgaggcaaa
     1861 tcctgaaatc gcaaacacaa ctcgcaaaat ttgcgtatct gcggatgctg caagtccagc
     1921 ccggcatcct gaaactgccg ctccagtgtg tcgttggcat ccttgtattc ccacgcaccc
     1981 accagcgaag tcagccgatt caacacttcc ggatcgaacg caaaagcttt cccaatttcg
     2041 cgcgccgctg accgtccacg atacgtgatg acatttgccg tcatggccgc gccaagtttt
     2101 ccatagcgtt cataaacgta ttgaatggct cgttc
//
LOCUS       DATCBB010000465         1455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_275152, whole genome shotgun sequence.
ACCESSION   DATCBB010000465 DATCBB010000000
VERSION     DATCBB010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275152"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VII37_05140"
     CDS             complement(<1..389)
                     /locus_tag="VII37_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5069145.1"
                     /translation="MSSAFAEFKEMAVVNTLRSCQLFTGLPQPDLQNIANVSTLKSLA
                     KGEYLFHEGDAASGFYVIQRGAVNVHRVTATGKEQIIHVFRTGDSFAEVALASATGYP
                     ADARALEDTQILLVQKAGILALLKRQP"
     gene            complement(398..640)
                     /locus_tag="VII37_05145"
     CDS             complement(398..640)
                     /locus_tag="VII37_05145"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEY5069146.1"
                     /translation="MLPLTQFKRFDVRDLIRRGVEPFPEIHKRVNALKPDEGLIVVAP
                     FLPSPLVEKLAGEGFASKVERGNGADWLVYFWREAD"
     gene            complement(662..1045)
                     /locus_tag="VII37_05150"
     CDS             complement(662..1045)
                     /locus_tag="VII37_05150"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HEY5069147.1"
                     /translation="MNENQTSPVKKPGTVVYACSGCSDAGELADRIARQLSREGAAEM
                     SCLAGIGGRVKPLVNRAASAERILAIDGCPLNCTRHTLELAGFKTFDHLELHKLGMRK
                     GSCPVTEENIAAGVAAAKKILADVS"
     gene            complement(1213..>1455)
                     /locus_tag="VII37_05155"
     CDS             complement(1213..>1455)
                     /locus_tag="VII37_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5069148.1"
                     /translation="DEAVFDEPDKFRLDRDPAKNLLYGTGIHYCPGAPLARLEMRVFL
                     EELLARTREMILVPEKSATISVYPASGFATLPLRIR"
BASE COUNT          312 a          412 c          412 g          319 t
ORIGIN      
        1 tccggctggc gcttgagcaa tgccagaatg ccagcctttt ggacaagcag aatttgtgtg
       61 tcttctagtg cgcgggcgtc agcgggataa ccggtagccg atgccagcgc cacttcggca
      121 aaggaatcgc cggtgcgaaa aacgtggatg atttgttcct tgccggtggc ggtcacgcga
      181 tgcacgttca ccgctccgcg ttgaatgaca taaaatccgc tcgccgcatc accttcgtga
      241 aacagatatt cacctttcgc cagagatttc aaagttgaga cgttggcaat gttttgcaaa
      301 tccggctgcg gcaagccggt gaaaagctgg cagcttcgca gcgtattgac gacggccatt
      361 tctttgaatt cggcgaacgc ggacgacatg acttcattca atcagcttcg cgccaaaagt
      421 aaaccagcca gtctgcgccg ttgccgcgct caaccttgct ggcaaagcct tcaccggcca
      481 gtttctcgac cagcggcgac ggcaggaacg gcgcgacgac gatcaagcct tcatccggct
      541 tgagcgcgtt cacgcgttta tgaatttctg gaaatggctc gacaccgcgc cggatcaggt
      601 cgcgcacgtc aaagcgtttg aattgggtca gcggcagcat gattcaatca gttagggagg
      661 gttacgaaac gtccgcaaga attttcttcg ctgcggcaac gcccgccgca atgttttctt
      721 ccgtcaccgg acacgaacct ttgcgcatac cgagcttgtg cagttcgaga tggtcgaaag
      781 ttttgaaacc ggcaagttcc agcgtgtgcc gcgtgcagtt cagtggacag ccgtcaatcg
      841 cgaggatgcg ttccgccgag gccgcccggt tgaccagcgg cttgacgcga ccgccgatgc
      901 ccgcgagaca ggacatttcc gccgcgcctt cgcgcgagag ttggcgcgcg atgcggtcgg
      961 ctagttcgcc cgcgtccgaa caaccggaac aggcataaac caccgtgccc ggcttcttca
     1021 ctggtgaagt ctgattctca ttcatggtat cgtatttgtt gtcatgacac taatctgccc
     1081 caaacgccag cgggatgatt tgacgcaggt caataagtta cgactttgtc ggtcaggcaa
     1141 caaatgccct tctttgcttg agtcagtttc acaattgaat tgaatgcgcc gtctatttca
     1201 gcgagccacc gttcaacgga tgcgcagcgg cagggtggca aagccgctgg caggatagac
     1261 cgaaatcgtg gcggacttct ccggcacgag gatcatttcg cgggtgcggg ccagtaactc
     1321 ttccagaaat actcgcatct ccagtcgggc caacggtgcg cctggacagt aatgaatgcc
     1381 cgtgccgtaa agcagatttt tggccggatc gcggtccagg cggaacttgt ccggctcgtc
     1441 aaacacggct tcatc
//
LOCUS       DATCBB010000466         1180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_275153, whole genome shotgun sequence.
ACCESSION   DATCBB010000466 DATCBB010000000
VERSION     DATCBB010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275153"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VII37_05160"
     CDS             <1..248
                     /locus_tag="VII37_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY5069149.1"
                     /translation="TKSMTGHLLGGAGGLEAGIAVLALRDQVIPPTINLESPDPECDL
                     DYVPNVARKATLDYALSNSFGFGGTNAALIFKRWSGK"
     gene            289..1053
                     /locus_tag="VII37_05165"
     CDS             289..1053
                     /locus_tag="VII37_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY5069150.1"
                     /translation="MKIIVCIKQVPAKDAPLSIAGNWIKESDIGFEMNEPDSYALEEA
                     LRLKEKHGGEVIAISMGPERVKQTIKEGLAKGADRGIHISDDQFAQLDPLNSARMLAE
                     ALRKENADLILTGLQSDDHGFGQTGVLIASLLDLPHATIIMQIEVQEKRMRLKRELEA
                     GWFQHIECPLPAVLSIQSGINKVRYATLKGIMAAKKKEIASITRESLGVKTEPTQKIE
                     KIYVPTKTKKTEFLTGTPTEMATKLVEKLKFDARVI"
BASE COUNT          323 a          301 c          305 g          251 t
ORIGIN      
        1 caaccaaatc catgaccggc catttgcttg gcggtgccgg cgggttggag gccggtatcg
       61 ccgtacttgc gcttcgcgat caggtgatcc cgccaaccat taacctcgaa tcgcccgatc
      121 ccgaatgcga tctggattac gtgccgaatg tcgcgcgcaa agccacgctg gattacgcgt
      181 tgtccaattc gtttggattt ggcggcacca atgccgcgct aatttttaag cgttggagcg
      241 gcaaataagc ccttcgacgc cggtcaagtt tttgtcagga aaaatatcgt gaaaattatt
      301 gtttgcatta agcaggttcc cgccaaggat gcgccactct ctatcgccgg taactggatc
      361 aaggaatccg acatcggatt cgaaatgaac gagccggaca gttacgcgct agaagaagct
      421 ttgcgcttga aggagaagca tggcggcgaa gtgatcgcga tttccatggg tccggagcgc
      481 gtgaaacaaa ccattaaaga gggtctggct aagggcgccg accgcggcat tcacatttca
      541 gacgatcagt tcgctcaact cgaccccctt aactccgcgc gaatgcttgc ggaagccttg
      601 cgcaaagaaa atgctgattt aattttgacc ggtctgcaaa gcgatgatca cggttttggc
      661 cagaccggcg tgcttatagc ttcgcttctt gatctcccgc acgccaccat catcatgcaa
      721 atagaagtac aagaaaaaag gatgcggctg aagcgggaat tggaggctgg atggttccag
      781 cacatcgagt gcccgctgcc cgccgtgcta tccattcagt cgggcattaa caaagtccgc
      841 tacgccacgc tcaagggcat aatggccgcc aagaaaaaag aaattgcttc cataacgcgt
      901 gaatcgcttg gagtgaagac tgaacccaca caaaaaatcg aaaagattta tgtgcctact
      961 aaaaccaaaa aaaccgaatt tctaaccggc actccgacgg aaatggccac caagctggtt
     1021 gagaaattga aattcgacgc gcgtgtcatt taatgagtta aatgaattca gcacgtggta
     1081 gggaatcgag accgtaaatg aagatcctgg taattaccga gcaacgccaa ggcaaatgga
     1141 ataacaccag ctctgaaacg ctggcggccg ctcagcagat
//
LOCUS       DATCBB010000467         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_469546, whole genome shotgun sequence.
ACCESSION   DATCBB010000467 DATCBB010000000
VERSION     DATCBB010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469546"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VII37_05170"
     CDS             complement(<1..1102)
                     /locus_tag="VII37_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY5069151.1"
                     /translation="MKSKSSKSKTFDPAPLTRRNLFEWAGLALAAATVPPAFSALSSS
                     QQSSTPKSSGSVMNALSSYMSEAANRPLPDEAVEKTKHHILDTLGAMISGSKLLPGQR
                     ALQFAQTYGGKEASTVVASNIVCGPIEAALTNGMLAHADETDDSHSPSQSHPGCAVVP
                     AAFAAGEQFQISGTHLLRAVALGYDVGTRVTMTLGGQKFEVESHWSTHAIAPHFGAAA
                     AAACAANLSPQEMRWMLGYTAHQSSGLGAWNRDPDHIQKAFLFGGMNARSGVTSALIV
                     KSGWTGVDDIFSGKDNFFQAYNPSANPSGLIDKLGERYEITRTNIKKWPVGSPIQAAL
                     DALELLRKQHELDANQVQQVKIRLATDEAAIVN"
     gene            complement(1164..2228)
                     /locus_tag="VII37_05175"
     CDS             complement(1164..2228)
                     /locus_tag="VII37_05175"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:NF006048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY5069152.1"
                     /translation="MKTFNIAVIAGDGIGKEVVPEGIRVLEAAGKRFGFGWKWHEFDW
                     SCETYVQTGKMMPDDGLEQLRPFDAIFLGAVGHPSVPDHVSLWGLLIPIRRTFRQYVN
                     LRPVRLFDGIESPLKNWKPGQIDFTIVRENNEGEYSNIGGRLYEGTEDEMAVQQTVFT
                     RRGVNRVLRFAFELARKRKKHLTSATKSNGIIHTMPFWDDCFRAMATEFPDIRTDQYH
                     IDILTAHFVRHPDWFDVVVGSNLFGDILSDLGPAIVGSIGIAPSANLNPEKEFPSMFE
                     PVHGSAPDIAGKGIANPIGQIWSGAMMLRHLGAEEGADSIEAAITTTLANSKVRTPDI
                     GGKSSTRDLGEAIAKQVSGN"
BASE COUNT          481 a          604 c          712 g          548 t
ORIGIN      
        1 tgttgacgat ggcggcttcg tcagtggcaa gccggatctt aacttgctgc acttggttag
       61 cgtcgagctc gtgctgtttc cggagaagtt ctagcgcatc gagggcggcc tggatgggag
      121 agccgaccgg ccactttttt atgttggtgc gggtaatctc gtaacgctcg ccaagtttgt
      181 cgatcaggcc ggagggattg gcactcgggt tgtaggcctg aaagaaatta tctttgccgg
      241 agaaaatgtc gtcaacgccg gtccacccag acttgacgat gagcgcagag gtgacgccgc
      301 tgcgcgcgtt catgccgcca aaaagaaatg ctttctggat gtggtcaggg tcgcgattcc
      361 aggcgccgag gccggaagat tgatgagctg tataacccag catccaccgc atttcctggg
      421 ggctcaggtt ggccgcgcag gcagcggcag ccgcggcacc aaaatgcggt gcgatggcgt
      481 gggtgctcca gtggctctcc acttcaaatt tttgcccgcc gagcgtcatg gtgacgcgag
      541 ttccaacgtc ataacccagc gcgacggctc gcaagagatg cgttccgcta atctgaaatt
      601 gttctccggc cgcaaatgcg gcgggcacca cagcgcaacc gggatgggac tgtgatggcg
      661 agtgagagtc atcggtctcg tcggcgtgcg ccaacatacc gttggtcagc gcggcttcga
      721 tcggtccgca cacaatattg gaggcgacga ccgtggacgc ttcttttccg ccgtaggtct
      781 gcgcgaattg taaagcacgc tggccgggaa gtaatttcga gccggaaatc atcgccccga
      841 gtgtgtcgag gatgtgatgc ttagtttttt caacggcctc gtcgggcaac ggacggttgg
      901 cagcttcact catgtacgag ctgagcgcat tcatgacgct gccgctggat ttcggcgtgg
      961 aggattgttg cgacgatgag agagcgctga atgcgggagg taccgttgcc gcggcaagcg
     1021 ccaagcccgc ccattcgaaa agattgcggc gagtaagggg cgccggatcg aaggttttgg
     1081 acttgctcga tttagatttc atgcggttgg catttttgcg taatggaatt gttcgctgca
     1141 ccagacaggt ttcaggtaga gcattaattt ccgctgactt gcttggcaat tgcttcacca
     1201 agatctcgcg tgctggactt tcctccaata tccggtgtgc gcacttttga attggccagc
     1261 gtggtggtaa tcgcagcttc aattgagtct gcgccttctt cagccccgag atgtcgaagc
     1321 atcatggcgc ctgaccagat ttggccgatg ggattggcaa tgccttttcc ggcgatgtcc
     1381 ggtgcggaac cgtggaccgg ctcaaacatg gaagggaatt ctttttctgg attgagattt
     1441 gcggatggcg cgattccgat cgatccgact atggctggac cgaggtcgga gaggatgtcg
     1501 ccaaagagat ttgaacccac caccacatcg aaccaatcgg gatggcgcac gaagtgggcg
     1561 gtgagaatgt cgatgtgata ttggtcggtg cggatgtccg gaaattccgt tgccatcgcg
     1621 cggaagcaat catcccaaaa gggcatggtg tgaatgatgc cgttggattt agtagcggaa
     1681 gtgagatgtt ttttgcgctt gcgggccaat tcgaatgcga agcgcagcac gcgattcacg
     1741 ccgcgacgcg tgaataccgt ttgttgaacg gccatctcgt cttcggtgcc ttcatacaga
     1801 cgcccgccga tgttggaata ttcgccttcg ttattttcgc gcacgatggt gaaatcgatt
     1861 tggcctggct tccaattttt cagcggcgac tcgatgccat cgaataagcg tacggggcgg
     1921 agattgacgt actgacggaa tgttcggcga atgggaatca agagtcccca aagcgaaaca
     1981 tgatcgggaa cgctcgggtg acccacggcg ccgagaaaga tggcgtcaaa cggccgcaat
     2041 tgctccagac cgtcatccgg catcattttt ccggtttgga cgtacgtttc acagctccaa
     2101 tcgaattcgt gccatttcca gccaaagcca aagcgctttc cggcggcttc gagcacgcgg
     2161 atgccttcgg gcaccacttc tttgccgatg ccgtccccgg cgatgaccgc gatgttgaaa
     2221 gttttcatgg tgccagttat cccgccgccg aagacaacat tggcgatagc gcggacatgt
     2281 ccggtgcaga ctccaacgct ttaacgaaga cgattatttt ttcggtcttg actttcggcc
     2341 actcg
//
LOCUS       DATCBB010000468         1259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_405053, whole genome shotgun sequence.
ACCESSION   DATCBB010000468 DATCBB010000000
VERSION     DATCBB010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405053"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VII37_05180"
     CDS             complement(<1..343)
                     /locus_tag="VII37_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069153.1"
                     /translation="MGFNDFVGNERIVNALRGALRAERVPHALLFTGASGVGKYTLAR
                     MFAQAATCERMKDDFCGECDTCKRVTLLADPVSLVQQGLAERGESADAAMVERTPLIL
                     QTHPDVWAVVPD"
     gene            complement(346..>1259)
                     /locus_tag="VII37_05185"
     CDS             complement(346..>1259)
                     /locus_tag="VII37_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069154.1"
                     /translation="VNYETEKKGRPRFIPQLRTWGTLPKLDCHTVLNRSTRADLRGGA
                     GAVTLAVNAGTRKFSTIPTISPAHRTMTTSRKLIAIEGIDGSGKRTQVELLEDALASR
                     GHQTFVTGFPIYDSWFGAMVGKFLDGGFGPLEEVDPQLTALLYAGDRFEAKKQLVDAL
                     AAGKIVLADRYVGSNLAHQAARVAAPARAEFVKWIEHLEYGIYGLPRESAIIYLRVPP
                     LEAQKLVGRKSTRGYTAATHDLLEENLRHLEDAAAMYDELAKRENWIRVECFDSSAHS
                     IRPPNAIAEEILTAVETVLAETRGENS"
BASE COUNT          269 a          443 c          273 g          274 t
ORIGIN      
        1 gatcgggcac caccgcccac acatccggat gcgtttgcaa aattagcggc gtgcgctcca
       61 ccatcgccgc gtccgcgctt tcccctcgtt ccgccaaacc ttgctgcacc aggctcactg
      121 ggtcggccaa cagcgtcaca cgtttgcacg tgtcgcattc cccacagaaa tcatccttca
      181 tccgttcgca cgtcgccgcc tgcgcaaaca ttcgcgccaa cgtgtatttg cccacaccgc
      241 tcgcaccggt aaatagcaag gcatgcggca cgcgctccgc gcgcaacgca ccacgcagcg
      301 catttacaat ccgctcattg ccaacaaaat cgttaaagcc catgcctacg agttctcccc
      361 acgcgtctcc gccagaaccg tttccaccgc tgtcaaaatt tcctccgcaa ttgcattcgg
      421 cggccttatc gaatgggcac tcgagtcaaa gcactccacg cgtatccagt tctcgcgctt
      481 cgccagttca tcgtacatgg ccgccgcatc ttccagatgc cgcaaattct cttccagcaa
      541 atcgtgtgtc gccgccgtat atcctcgcgt actcttccgc ccaaccagct tctgcgcctc
      601 cagcggaggc acccgcaaat aaattatcgc gctttcccgc ggcagcccat aaattccata
      661 ctccaggtgc tcaatccatt tcacaaactc cgcgcgcgct ggcgctgcaa ctcgcgcggc
      721 ctgatgcgcc aggttcgatc caacatagcg gtccgccagc acaattttcc ccgccgccaa
      781 tgcgtctacc agctgcttct tggcctcgaa ccggtctccc gcgtacagta gcgctgtcaa
      841 ttgcggatcg acttcctcca gcggcccaaa tccgccgtcc agaaatttcc ccaccatcgc
      901 gccaaaccac gaatcataaa taggaaatcc cgtgacaaaa gtctggtgtc cacgcgaagc
      961 caaggcatcc tccaacaact caacctgagt ccgcttccca ctcccatcaa ttccctcaat
     1021 agcaatcagt tttctgctgg tagtcatagt ccgatgtgct ggagaaatcg ttggaatggt
     1081 ggaaaatttc cgcgtgcctg cattgacagc cagtgtaacc gcgccagccc cacctcgcaa
     1141 atccgcccgc gtgctacgat ttaaaacggt gtgacaatcg agtttaggta gggtgcccca
     1201 ggttcgcagt tgaggtatga acctgggtct tccctttttc tctgtctcgt aattcacag
//
LOCUS       DATCBB010000469         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_209820, whole genome shotgun sequence.
ACCESSION   DATCBB010000469 DATCBB010000000
VERSION     DATCBB010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209820"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VII37_05190"
     CDS             complement(<1..1201)
                     /locus_tag="VII37_05190"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069155.1"
                     /translation="MSGKVCIIGGGGVRTPLLIHGFLQSQDILKISELVLFDVSHERA
                     EVMAALGREIASNMGVDIRLTTTSSLEAAVEGSTFVLSSVRIGGMAARARDERTAIEH
                     GLAGQETTGPGGVAMALRTVPVALEHARVVERLAPQAWFINFTNPAGLITQALNQHTN
                     LRIVGICDTPAELFHRIAHALGEPVSEAVCEYAGLNHLGWVRRVTVRGKDVTGQLLQN
                     PELLRQLYHASLFDPLLIQTLQLIPSEYLFFYYSQRKAYRNQLRAGATRGEELLRLNR
                     TLFAQLQAGTPREGLNTYRNYLMQRNSSYLRLEAEAGSALDEEREEIDPFEIATGYHR
                     IAIEVMTGLSSEEPRQVVVNVRNRGNIADLDDDDVVEVPCQISRNGVTPLSIGQLPEA
                     VRGLVLAV"
     gene            complement(1198..2124)
                     /locus_tag="VII37_05195"
     CDS             complement(1198..2124)
                     /locus_tag="VII37_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY5069156.1"
                     /translation="MSAARPLDVMVVGDLFAELVMSGFASWPGRAGDELFAERFCREI
                     GGGAAITACGLAKLGAKLGVVGVAGEADGQWLIERLESRGVNTSAIQRSSREPTAVTV
                     SVSGASDRTFLTYMGANRELPANLQQIASRGDFANARHIHLAFAPKPAEADNLFREII
                     SQGCTLSVDVGWHPEWLSDRDSREALRSVNIFFPNEREAALMTGETDPRLILQHFRKM
                     GLRTVALKLGPAGAGLLYDGTITFCEPITIQSLDTTGAGDCFDAGFLYAWLRGDSPRD
                     CLKAGTVCGALSTRCLGGIEGFPTIAEMDALI"
     gene            complement(2121..>2510)
                     /locus_tag="VII37_05200"
     CDS             complement(2121..>2510)
                     /locus_tag="VII37_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069157.1"
                     /translation="GRLHPTRNFPWVSLIFMGVTSAVASLLDLSDLITALVVIQVLVQ
                     FMAQVVAVTMIRRYRPDIHRPFQMWFYPVTSVIAFAGWAFILAASGLKFILWGIALIA
                     VGVAAYLWRARVQAEWPFGPAEAKGAM"
BASE COUNT          574 a          757 c          636 g          543 t
ORIGIN      
        1 tcacggccaa caccagtcca cgcacggctt ccggcaactg ccctatggat aggggggtaa
       61 ctccgttgcg gctaatttgg caaggtactt ccaccacatc atcgtcatcc aaatccgcga
      121 tgttgccgcg attgcgtaca ttcacgacta cttgtcgcgg ttcttcggac gacagaccgg
      181 tcatcacttc tatcgcgatg cggtggtagc cggtggcaat ttcaaacgga tcgatttcct
      241 ctcgttcctc gtcgagagcc gagcctgctt cagcctccaa tcgaagatac gaagagttcc
      301 gctgcatcaa ataattccga taagtattta agccctcacg cggtgttccg gcttgcagtt
      361 gagcgaatag tgttctattc agccttaata gttcctcacc ccgggtcgcg ccggcgcgca
      421 attggttgcg atacgccttt cgttggctgt aataaaaaaa taaatattcc gaaggaatca
      481 attgcagggt ttgaatgagc aacggatcga acaaactggc gtgatacaac tgtcgcaaca
      541 attcgggatt ttgcagcagc tggcccgtca catcttttcc acgcacagtg acacgccgca
      601 cccacccaag gtgattcaat cccgcgtact cgcataccgc ttcggaaacg ggctcgccta
      661 gcgcgtgcgc gatgcgatga aacaattcgg ccggcgtatc gcagataccc actatgcgca
      721 agttggtatg ttgattgagc gcctgcgtta taagtccggc gggattcgta aaattaatga
      781 accatgcttg cggcgctagt cgttcgacca cgcgcgcatg ttccagcgca actggtacgg
      841 ttcgcaatgc catggccaca ccgcccggcc cggtggtttc ctggccagca agaccatgtt
      901 cgatcgccgt gcgttcgtct cgcgcccttg cagccatgcc tccgatgcgc acgctgctta
      961 aaacgaaggt gctgccctcc acggccgctt ccaatgagga ggtcgtagtc agccgaatat
     1021 ccacgcccat gttgcttgcg atttcccgtc ccagtgcggc cataacttcc gcgcgttcgt
     1081 ggctaacgtc gaaaagtact agctcgctga ttttcagaat gtcctgcgat tggaggaatc
     1141 catgaatgag cagcggcgtt cgcacaccgc cgccgccgat aatgcacact ttaccgctca
     1201 tatcagagca tccatttccg cgatcgtcgg aaatccctcg attcccccaa gacacctggt
     1261 ggagagggcg ccgcacacag ttcccgcttt gagacaatct cgtgggctat ctccgcgcag
     1321 ccacgcatac aaaaatcccg catcaaagca atcacccgcg ccagtggtat cgagtgattg
     1381 aatcgtgatt ggctcgcaaa acgtgattgt gccgtcgtac agcagccccg ctcctgccgg
     1441 ccctagtttg agtgctacgg tccgaaggcc cattttcctg aaatgctgca gaatcagccg
     1501 cgggtcagtt tcaccggtca ttagggctgc ttctcgctca tttgggaaga aaatgtttac
     1561 gctgcgaagc gcctccctag agtctctatc ggaaagccat tccggatgcc agcctacatc
     1621 gacagaaagc gtgcaacctt gcgagattat ctctctgaat aaattatctg cttcggccgg
     1681 ttttggagcg aacgcgagat gaatgtgccg ggcgttggca aaatctccac gcgacgcaat
     1741 ttgttgcaaa ttcgctggca gttcgcggtt agctcccatg taagttaaga aggtgcgatc
     1801 actagcgcca gatacactca cggtcactgc agtgggctcg cgcgagcttc gctgaatggc
     1861 agaggtattt acgccgcgcg attccagcct ctcgattaac cattgaccgt cggcttcgcc
     1921 ggcgactccg accacgccta gcttggcccc caacttcgcc aagccgcacg ctgtgattgc
     1981 tgctcctccg ccgatttccc ggcaaaaacg ctccgcgaat aattcgtccc cggcacgtcc
     2041 tggccatgat gcgaagccac tcatgaccag ttctgcgaac aggtcaccta cgaccattac
     2101 gtccagcggc cgggcagcac tcacatggct cctttagctt ccgccggccc gaacggccat
     2161 tcagcctgca cccgcgcacg ccacaggtaa gcagcaacgc ccaccgcgat cagagcgatt
     2221 ccccagagaa taaatttcag tccactggct gcgagaatga aggcccaccc tgcaaaagcg
     2281 attacgctgg tgaccggata aaaccacatt tgaaacggcc ggtgaatatc ggggcgatat
     2341 cgacggatca tggtcaccgc cacgacttga gccataaact gcacgagcac ttggataacg
     2401 accaacgcgg taatcagatc ggataaatcc agcaaactcg caactgcgga agtgacgccc
     2461 ataaatatca gggaaaccca cggaaaattt cgagtgggat gtaagcgccc
//
LOCUS       DATCBB010000470         1659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_241648, whole genome shotgun sequence.
ACCESSION   DATCBB010000470 DATCBB010000000
VERSION     DATCBB010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241648"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VII37_05205"
     CDS             complement(<1..351)
                     /locus_tag="VII37_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069158.1"
                     /translation="MSAGKTFRHGQILRLVSDGSIANQEDLRRRLAAQRLRVTQATLS
                     RDIQELRLIKTQRGYKSASALPEDLSPLPPLARALGEFLLDIRPAENLLVLKTPPSGA
                     QPLAAAVDGSKFPEI"
     gene            613..921
                     /locus_tag="VII37_05210"
     CDS             613..921
                     /locus_tag="VII37_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069159.1"
                     /translation="MPPALREQQVCVLHFTLSVENSCAGMRRETALGNAPHERQREIM
                     KFIAEHGERLARVATSGLPLTDDLKARILSTFLTLMNLRENLDRSNMRSSFGRTSQLR
                     "
     gene            1070..>1659
                     /locus_tag="VII37_05215"
     CDS             1070..>1659
                     /locus_tag="VII37_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY5069160.1"
                     /translation="MTLRTSFRVVSVFIVAVLIFGLHPIYTAASHARIIRLSLVQGDV
                     RILHDSKGDPLANDKAPWERAELNLPIRQHDVLATDNGRAEVEFENGAMAFINENSVL
                     EFFDLSLDDGSLTTRLILRQGTGSFYVNPARDDYFSVTGGDFTAEAAGRANFRMDNFD
                     EGSTVAVVKGQVNVVHKNDTKLLAKGQSFTMSASGAT"
BASE COUNT          365 a          463 c          441 g          390 t
ORIGIN      
        1 aatttccggg aatttggatc catccacggc cgcagcaagt ggttgcgctc cgctgggcgg
       61 cgtcttgagc accaagagat tttcggccgg ccgaatatcc agcagaaatt cacccaatgc
      121 tcgagccaac ggcgggagcg gcgatagatc ttccgggagc gcggaagccg atttgtaacc
      181 acgctgcgtt ttgatcagtc ggagttcttg aatatcgcgc gacaacgtag cctgcgtcac
      241 gcgcaaccgc tgcgccgcga gtcggcgacg cagatcttct tgattggcga tagaaccgtc
      301 gcttactagc cgaagaattt gaccatgacg aaacgttttg cctgcgctca ccggatcctc
      361 aaatacaaat atacagtcac atgtattttt atacagttac gcgtcgccag agtcaacgct
      421 ttaatgaatt tcccaggtgt ctactgttcg aacgaatggg gaacgtaact ggcccgcagg
      481 gcagtggtgt agcattcgtc gcactggccc gacacatact ggattttacg tgcgtaggat
      541 gttacctatc atgaacagca tctttagcgc tctatgacga acgcttgcga agtcacaggc
      601 tgtaagatgg aagtgccacc ggctctgcgt gagcaacagg tgtgcgtact gcattttact
      661 ttgtcggtgg aaaatagttg cgcgggcatg cgccgggaga cggcgctagg caatgccccg
      721 cacgaacgcc agcgcgaaat catgaaattc atcgccgaac atggcgagcg cctggcgcgc
      781 gtagccacca gcggcttgcc gctcaccgac gatctgaaag cgcggatcct tagcactttt
      841 ctcaccctca tgaacctgcg cgaaaatctc gatcgctcca acatgagaag ttccttcggc
      901 cgcaccagcc aattgcgcta gtttctcgcg ccgcaagcct cgtaagatct tacattctgt
      961 tgactttgct gcacttgacg ccgcgtatac cgcgagagac catatcttta gcagcatcag
     1021 cgccggaggc cgccattctc gaggctgctc acccggcagg ggtaatccaa tgactctccg
     1081 tacttctttc cgcgttgttt ctgtctttat cgttgctgtg ctgattttcg gacttcatcc
     1141 catttatacc gccgcgagtc acgcgcgcat tatccggttg agtctggtgc agggagatgt
     1201 gcgcattctt catgattcca agggcgatcc gctagccaac gacaaagcgc cttgggaacg
     1261 cgccgaattg aatctgccca ttcgccagca tgatgtgctg gccaccgata atggccgcgc
     1321 cgaagtggaa ttcgaaaatg gcgcgatggc cttcattaac gaaaattcgg tgctcgaatt
     1381 tttcgacctt tctcttgacg atgggtctct gacgactcga ctcattcttc gtcaaggcac
     1441 cggttccttt tatgtgaatc cggcccgtga cgactatttc agcgtcactg gcggtgattt
     1501 cactgctgaa gcggccggcc gcgcaaattt ccgcatggac aatttcgatg agggcagcac
     1561 cgtagcggtc gttaaaggcc aagtgaacgt agtgcacaag aacgacacca agcttttggc
     1621 gaagggccaa tcttttacaa tgtccgcgag cggcgctac
//
LOCUS       DATCBB010000471         3244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_145621, whole genome shotgun sequence.
ACCESSION   DATCBB010000471 DATCBB010000000
VERSION     DATCBB010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145621"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..547
                     /locus_tag="VII37_05220"
     CDS             191..547
                     /locus_tag="VII37_05220"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEY5069161.1"
                     /translation="MDNSVGLVAVILSLGFPLGAMYTYYCVRKLRSQERLAAMARGVE
                     IPMEPELPQGARSRRWGILLVSVALGYMAAAGIIARFAEPDAWTAAALGIIPFALGLG
                     FFVDWTLIRRDARVSS"
     gene            608..1390
                     /locus_tag="VII37_05225"
     CDS             608..1390
                     /locus_tag="VII37_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069162.1"
                     /translation="MHPAIPHLIELQRVDHTIGTLRAEIDSYPKRIQEADSKLSGARA
                     AVAAAKEAHTNALKERKKFELDVDQWKDRARKYRDQSGAVKTNEAYKALLHEIANAEA
                     EVAKAEDRQLDVMMSGEEFERRVRHAEADLKEAETSVAADRKEIHARYDEKQKLMKAA
                     LAERERIAAPVPKDLLELYERIAKRHNGTAMAQVRDDQCRGCGMRVLPHIIQELRQET
                     NEEIYRCEMCGLILYTLDPIPVANSGPGSGSDKSSATSAINS"
     gene            1390..2043
                     /locus_tag="VII37_05230"
     CDS             1390..2043
                     /locus_tag="VII37_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HEY5069163.1"
                     /translation="MIRRPTSSRETGLFAGSEPEAKPRAAAFQANIDGGSRGNPGPAS
                     YGVVVRNERGEIVAKLKKYIGRMTNNVAEYYGLIAALDYAQSSGVRALRIESDSELLV
                     RQMRGHYKVKSPDLRPLFERAKKMSQTFESFRIDHVYREQNAEADALANEALDETSGK
                     PSVTTKAEGRPKLPGDMQSAPRKLRARVRNGGLHFLEPLDLPENTEVEILLRPAPKN"
     gene            complement(2055..3089)
                     /gene="mqnC"
                     /locus_tag="VII37_05235"
     CDS             complement(2055..3089)
                     /gene="mqnC"
                     /locus_tag="VII37_05235"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEY5069164.1"
                     /translation="MSLTKQEALDMLESDDLLGIGMAAHQMRLKKNDPRVVTYQIDRN
                     INYTNFCTEYCSFCAFYRPLGAKDGYLLPFESIYEKIDEMLELGGTGVLLQGGLHPDL
                     QIGYYENLLRSLKQRYPQVHLHCFSAPEILCIAEVSELSVRETIQRLVEAGLQSIPGG
                     GAEILDDEIRSKIARLKCTSDDWEDMHRIAHSLGLRTTATMMFGCGEELRHRVNHLER
                     LRTIQEDTGGFTAFIPWMFAPDNTPLGKKIAEATAVDYLKTLAVSRLYLDNFDHIQSS
                     WLTPGIKVCQVGLQFGADDVGSILIEENVVFAAGVRNRTNESELRRIISDAGFVPAQR
                     DTLYRSYALK"
BASE COUNT          735 a          857 c          934 g          718 t
ORIGIN      
        1 ccccaatctg gccatcgatc aaaatatgca ggttcacgaa gtaaactagc ggaccggcag
       61 ccccggttta tcacggaaca tctttgcgga acattcctcg caacccctcc gtatcattta
      121 gcgacttcgg gcaacgaacg cgagcctggg ctactcaagg caattgcact tcccgaaaag
      181 gagaaggaat atggacaact cggttggact ggtcgcggtc attctctctt taggatttcc
      241 tctgggcgcc atgtacacct actactgtgt gcgcaaactt cgtagccaag agcgtctggc
      301 ggccatggcg cggggcgtcg agattcccat ggagccggaa ctccctcagg gagctcgttc
      361 gcggcgttgg ggcattctgc tggtttctgt tgcgctgggt tatatggccg ccgcgggcat
      421 catcgcccgc tttgcggaac ctgatgcctg gactgctgcg gccttgggca tcattccgtt
      481 cgctcttggg cttggctttt tcgttgattg gacgctgatc cgtcgggatg ctcgcgtttc
      541 gagctagcgt ttcctggcgc tggcgcaacg aggtccacga gcgcggccgt gttagaattg
      601 tccctcaatg caccctgcaa taccgcacct tatcgagctg caacgggtgg accacaccat
      661 tggcacgttg cgtgctgaaa tcgacagcta tcccaaacgg attcaggaag ccgacagcaa
      721 gctgagcggc gcacgtgcag ctgtcgcggc ggccaaagaa gcgcatacga acgcgctcaa
      781 agagcggaag aaattcgagc tagacgtcga tcaatggaaa gaccgggctc gcaaatatcg
      841 cgatcagagc ggcgcagtaa aaactaatga ggcttacaaa gcgttgctcc acgagatagc
      901 taatgccgaa gcggaagtag ccaaggctga agatcgccag ctggatgtga tgatgtccgg
      961 agaagaattt gagcggcgcg ttcggcacgc cgaggcggac ctgaaggaag cggaaaccag
     1021 cgtggcggcc gatcgcaagg aaattcatgc acgctacgat gaaaaacaaa aattgatgaa
     1081 ggcggcatta gcggaacgcg agcgcatcgc tgctccagtg ccgaaagatt tgctggagct
     1141 ctatgagcgc atcgcgaagc ggcacaacgg cacggcgatg gctcaggtgc gcgacgatca
     1201 atgtcgcgga tgtggaatgc gcgtgcttcc gcatatcatt caggagcttc gacaggaaac
     1261 taacgaagaa atctatcgct gcgaaatgtg tggcttgatc ttgtacaccc tggatcctat
     1321 tccagtggca aattccggcc caggctcagg cagcgacaag tcctccgcta cctcagccat
     1381 aaattcctga tgattcgtcg cccgacctct tcacgcgaaa ccggattgtt tgcgggttcg
     1441 gagccggagg caaaaccgcg cgccgccgcc tttcaagcta atatcgacgg gggctcgcgc
     1501 gggaatccgg ggcctgcttc ctacggcgtg gtggtccgca acgagcgcgg agagattgtc
     1561 gcaaaattaa aaaaatacat cgggcgcatg acgaacaatg tcgccgagta ctacggcctg
     1621 attgccgcgc tggactacgc gcaatcgagc ggcgtgcgtg cgttgcgcat cgaaagcgac
     1681 tctgagctgc tggtgcggca gatgcgcggc cattacaaag taaaaagccc cgatctgcgt
     1741 ccgttatttg aacgggccaa aaagatgtcg cagacgtttg aatcgtttcg catcgatcac
     1801 gtatatcgcg agcagaatgc cgaggctgac gcgcttgcga atgaagcgtt ggatgagaca
     1861 tcgggcaagc cttccgtcac gaccaaggcg gaagggcgcc cgaagttgcc cggagacatg
     1921 caaagcgctc cacgaaaact gcgcgcgcga gtccgcaacg gtggattgca ttttcttgag
     1981 ccgttggatt tgccggaaaa taccgaagtt gaaatcctgt tgcgacctgc acctaaaaat
     2041 taaatcggag ccatttactt cagggcatac gagcggtata gagtgtcgcg ctgcgcgggg
     2101 acgaaaccgg catcagagat tatgcggcgc agttcgcttt cgttggtgcg gtttcgaacg
     2161 ccggcggcaa acacgacgtt ttcttcaatc aatatgctgc caacgtcgtc tgcgccaaac
     2221 tgcaagccga cctggcacac ttttatccca ggggttagcc aactcgattg aatgtggtcg
     2281 aaattatcga gatatagccg gcttaccgcg agcgtcttca agtaatccac cgcggtggct
     2341 tcggctattt tttttccgag tggagtatta tcgggcgcaa acatccacgg aataaaagcc
     2401 gtaaagccgc cggtatcttc ctggatcgtg cgcagtcgtt cgaggtgatt tacgcggtgc
     2461 cgcagctctt cgccgcaacc gaacatcatt gtggcggtgg tgcgcaaacc gagcgaatgt
     2521 gcgatgcggt gcatatcttc ccagtcatca ctggtgcatt tgagccgggc gattttggat
     2581 ctgatttcgt catccagaat ttccgcgccg ccgccgggta tagattgcag gccggcttcc
     2641 accagtctct gaatggtttc gcggacactg agctcgctga cttccgcgat gcatagaatt
     2701 tccggcgcgg agaaacaatg cagatgcact tgcggataac gttgttttag agaacgcagc
     2761 agattttcgt agtagccgat ctgaagatcg ggatgcaggc cgccctgcaa tagaacgcca
     2821 gtgccgccca gttcgagcat ctcgtcgatt ttttcgtaaa tcgattcgaa tggcagcagg
     2881 taaccatctt ttgcgcccaa cgggcgatag aaagcgcaga aagaacaata ttcagtgcaa
     2941 aaattcgtgt aattgatgtt gcggtcgatc tggtaggtga ccacgcgcgg gtcattcttt
     3001 ttgaggcgca tttgatgcgc agccattccg atgcccagca ggtcatcgga ttccagcatg
     3061 tccagcgctt cttgttttgt caggctcaag ggtttccttg cgtaaatctt attctatcga
     3121 atgagctcta tgaatccgaa acctgttggc ctcgcttgcc agcagtttat tgcgatgcat
     3181 atcgaggggt gttgatcgtg gcccattcga tggccttggt tctcggtatg agttccagct
     3241 tggc
//
LOCUS       DATCBB010000472         1189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_241657, whole genome shotgun sequence.
ACCESSION   DATCBB010000472 DATCBB010000000
VERSION     DATCBB010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241657"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VII37_05240"
     CDS             complement(<1..1188)
                     /locus_tag="VII37_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5069165.1"
                     /translation="MSGVIREAVIAGALTGLLILLFLGSWRSTLIITISIPLSILASI
                     CCLSALGETINIMTLGGLALAVGILVDDATVTIENINYHLEQGKDVEAAILDGAHQIA
                     LPALVSTLSICIVFVPMFLLAGIAKFLFIPLAEAVVFAMLTSYLLSRTLVPTLAKFWL
                     KKHDAKRKQPGFLSRFQLRFERGFERFREHYHSLLGSALQSGRRFAAIFLGAMIATAI
                     LAFPLGPLPGLGQDFFPSVDGGQIKLHFRTRTGTRIEETAALCDRVEATIREIVPAKQ
                     ISSIVDNLGLPYSGINLSYSTSAPVGAGDADVYVNLTEDHKPTLQFVRALRKKLTATY
                     PSTQFSFLPADMIGQILNFGLPAPIDIQIIGENVQANHEFANGLLQKLHAVAGAVDLH
                     IHQS"
BASE COUNT          289 a          302 c          353 g          245 t
ORIGIN      
        1 tgattggtga atgtgcagat ccacagcacc cgcgacggca tggagtttct gcaatagtcc
       61 attggcaaat tcgtgattgg cttgaacgtt ttcgccgatg atctggatat cgatcggggc
      121 gggaagtccg aaatttaaaa tctgtccgat catatcggct ggcagaaagg aaaattgggt
      181 ggacggatag gttgcggtga gtttcttgcg cagggcgcgg acgaattgca gcgtgggctt
      241 gtgatcttca gtgaggttga catagacatc cgcatcgccg gcgcccactg gagccgaagt
      301 gctataagac agattaattc cgctataggg cagacctagg ttatcgacga tgctgctgat
      361 ttgcttggct ggaacaatct cccggatcgt cgcctcgacg cgatcgcata gggcggcagt
      421 ctcttcaatg cgtgtgccgg ttcgcgtcct gaaatgcagc ttgatttggc cgccgtcgac
      481 tgacgggaag aagtcctgtc ccaagcccgg cagcggcccg aggggaaagg ccaaaattgc
      541 ggtggcaatc atggcgccta gaaaaatggc ggcaaaacgc ctgccgctct gcagcgctga
      601 gccgagcagc gagtggtagt gttcgcggaa gcgctcgaat ccgcgctcga atcgcaattg
      661 aaagcggctt aagaatcccg gctgcttgcg cttggcatca tgtttcttta gccaaaattt
      721 cgcgagcgtc ggcaccaagg ttcgcgacag caagtaagaa gtcagcatcg cgaacaccac
      781 tgcttccgcc aagggtatga atagaaattt ggcgatcccg gccagcaaaa acatcggcac
      841 gaacacaatg catatggaaa gggtggaaac gagagccggc aaggcaatct gatgtgcgcc
      901 gtcgagaatg gccgcttcga cgtccttgcc ctgctcaagg tggtagttga tattttcgat
      961 ggtcacggtt gcatcatcaa ccagaatacc caccgccagc gccaagccgc ctaaagtcat
     1021 gatgttgatg gtttcgccca acgccgacag acaacagatc gaggcgagaa tcgatagggg
     1081 aatcgaaatc gtaatgatca gcgtgctgcg ccagctgccg agaaacagca ggatcaacaa
     1141 tccggtgagc gcgcctgcga tcactgcctc gcgtatgacg ccggaaatg
//
LOCUS       DATCBB010000473         1255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_372002, whole genome shotgun sequence.
ACCESSION   DATCBB010000473 DATCBB010000000
VERSION     DATCBB010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372002"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..569)
                     /locus_tag="VII37_05245"
                     /pseudo
     CDS             complement(1..569)
                     /locus_tag="VII37_05245"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
     gene            complement(693..>1255)
                     /locus_tag="VII37_05250"
     CDS             complement(693..>1255)
                     /locus_tag="VII37_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069166.1"
                     /translation="GMPNFAFLGDAKIEALVSYLRVLQGTSVPLPMPGNPQKGEVLFG
                     GTAQCAQCHMARGAGGFIATDLSAFAAGSSPADIRRAIVNTAQDGRQYRGKVVITFAN
                     GSSQEGVVRNEDNFSLQLQSLDGTFHLVQKAEISAVKPAEQPLAHADYGKTLTPGQLD
                     DLVSYLMHVAEKEPPKDPSKRKHQTD"
BASE COUNT          249 a          347 c          355 g          304 t
ORIGIN      
        1 atataaagct tttttccgtc gggagaaaag gccagcccgt tgggttgcga cagctccttt
       61 gttagaagcg tgacttttcc tttggaatcg aggcgaaaca cgccttgaaa cgctagttcc
      121 tgcttttcgc ctttgggtaa atccaacgtc gggtcggtga aatactaagc gccgtcaggg
      181 cccaggacca cgtcattagg gctgttgaat tttttccctt cgaagcgctc cgccagagtt
      241 tcgtatttgc cgtccgggct gatgcggatg atggcgcgca gcacgctggc gcagtcgatc
      301 aggcggtgct cgcggtcgta ggtgctaccg tccgggtcgc ccagcgcgat gagttctgat
      361 ttcttgccat cgagcgaaag tttatagatt ttgttctgct cttcgtcgct gacatacaga
      421 aaccctgcgg ggtcccaaac cggtccctcg gtgaacccaa aacccttgcc gaccgtctcc
      481 agtttggcgc tcttcggaat caggttccag aactgcggcg tatcagcgtg aagcgaaaat
      541 tctcgaggtt ttttggtttc ttgagccata gagatgctac cggctattag gagtccaacg
      601 gcaagtgtca gagctatttt caagagatct ccagcatgtg gcgcttaacc taaataccaa
      661 gtcatcggcg ccgcgcacca cgcgaatgaa aatcagtccg tttgatgctt tcgtttcgat
      721 ggatccttcg gcggctcttt ttctgctacg tgcatcaggt agctcaccaa gtcgtcgagt
      781 tggcccggcg tcagagtttt gccgtagtcc gcgtgcgcca ggggttgctc cgcaggcttc
      841 acggcggaaa tttccgcctt ttgcaccaga tgaaatgtgc cgtccagcga ttgcaattgc
      901 agagagaaat tatcttcgtt gcggactacg ccttcctgtg aacttccgtt ggcgaaagtg
      961 atcaccacct tgccgcgata ctgccggccg tcttgcgcgg tattcacgat ggcccgccgg
     1021 atgtccgccg gcgagctacc ggcggcaaaa gcggagaggt ccgtcgcgat gaagccgccg
     1081 gcgcccctgg ccatgtggca ttgcgcgcat tgcgcggtgc cgccaaaaag aacttcgccc
     1141 ttctgaggat ttcccggcat tggtaaagga acgctggtgc cctgaagaac gcggaggtaa
     1201 ctgaccagcg cttcaatttt ggcgtcgccc agaaacgcaa aattgggcat gcccg
//
LOCUS       DATCBB010000474         2139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_80065, whole genome shotgun sequence.
ACCESSION   DATCBB010000474 DATCBB010000000
VERSION     DATCBB010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80065"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..543)
                     /locus_tag="VII37_05255"
     CDS             complement(133..543)
                     /locus_tag="VII37_05255"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance family protein"
                     /protein_id="HEY5069167.1"
                     /translation="MNEFECVIPILNVKNFAVSMDYYVSKLGFRKKWDWGEPPTFGCV
                     TRGKVEIFLCEGAQGQPGVWMSIFLDDVDALHEEYRSSGAIIRQPPTNMPWGTREMNV
                     QDPDAHRFRMGSHSTGPTDEDGLKRFSEIEQFGK"
     gene            complement(702..1964)
                     /locus_tag="VII37_05260"
     CDS             complement(702..1964)
                     /locus_tag="VII37_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY5069168.1"
                     /translation="MRFSRRYFLKQGGVAMVGLSAMPAFLQRAVAATPMPNKKQLVVL
                     FQRGAADGLNIVVPFAEPNYYRLRPTIAIPQPRRGVVGEASIDLDGYFGLHPSLAPLE
                     PLFHKNQLAIVHAAGSPDPTRSHFDAQDFMESGTPGVKATDDGWLDRALQTIPEENAS
                     PFRAVAMGPSLPRMLRGSAGAIALPDLKNFKVMPQSAAMNNVVQGGFEAMYSQSVDHA
                     LHGTGTETFEAIDMLSKIDTSKLPPENGAQYPTSRLGQNMQQIGQLLKAKVGVEVLFV
                     DCGGWDNHVNEGGVQGQLSNLLKDLGQSLAAFHQDMGDRMQDIVVVTMSEFGRTAKEN
                     GNRGTDHGHANCMFVMGGEVKGGKVYGKWPGLDDHQLNDGRDLALTTDFRYVLGETLT
                     KHIGVKDLATVFPGFDNSAHRFPGLIKT"
BASE COUNT          420 a          567 c          640 g          512 t
ORIGIN      
        1 ttttctggct ggtgcgaatc cgcttcgcaa atgcatacgg gagaaggccg atacaagttg
       61 cgcccgcaag tgttgattaa ggtggcgggc tttttcgttt gccctgaggg gggcggcagg
      121 cgattcatgc gtttacttgc cgaactgttc aatctctgag aagcgcttca agccatcctc
      181 atctgtcggg cccgtcgagt gggagcccat gcgaaagcgg tgagcgtccg ggtcctggac
      241 attcatctct cgtgtacccc atggcatatt tgtcggtggc tggcgaatta tcgcgccgct
      301 gcttctatat tcctcatgca gcgcatccac atcatccaaa aaaatggaca tccacacgcc
      361 gggctgcccc tgcgctcctt cacagagaaa aatctccacc ttgccgcgtg tgacacagcc
      421 gaatgtgggt ggctcgcccc agtcccactt tttgcgaaag cccagcttgc tgacgtaata
      481 gtccatggag acggcaaagt tcttaacatt caagatggga atcacgcatt caaattcatt
      541 catataacga ctccggctag aagcataaga ttaccaaacg cccaaactct ggccagtttt
      601 cattcatatg tcaaattaat gatgcgctaa acggacaagt gcgtgttgtt catgtccggc
      661 tgatttcact tcggttttac cgctgtgagc gcgcctggat attaggtttt gatcaggccg
      721 gggaagcgat gggcgctgtt gtcgaagccg gggaataccg tggctaggtc ttttacgccg
      781 atgtgtttgg ttagggtttc gccgaggacg tagcggaagt cggtggtcag ggcgaggtcg
      841 cggccgtcgt ttagttgatg atcgtcgagg ccgggccatt tgccgtagac tttgccgccc
      901 tttacttcgc cgcccattac gaacatgcag ttggcgtggc cgtggtcagt gccgcgattg
      961 ccgttttctt tggcggtgcg tccgaactcg ctcatggtga cgactacgat gtcttgcatg
     1021 cggtcgccca tgtcctgatg aaaggccgcg agggattggc cgaggtcttt caggagattg
     1081 gagagctggc cttgcacgcc gccttcgttg acgtggttgt cccagccgcc gcaatcgacg
     1141 aacaaaactt ccacgccgac tttggctttc agaagctggc cgatttgttg catgttttgg
     1201 cccaggcggc tggtgggata ttgcgcgcca ttttcaggcg gcaatttgct ggtgtcgatt
     1261 ttgctgagca tgtcgatggc ttcgaaggtt tccgtgccgg tgccgtggag ggcgtggtcg
     1321 acgctttgcg aatacatggc ttcgaagccg ccctgcacca cgttgttcat cgccgccgac
     1381 tgcggcatga ccttgaaatt tttcagatcc ggcaaggcaa tcgcgccggc gctgcctcgc
     1441 agcatgcgcg gcagacttgg ccccatggcc acggcgcgaa atggcgaagc attttcttcg
     1501 gggatggttt gcagggcgcg gtcgagccag ccgtcatcgg tggcttttac gccgggcgtg
     1561 ccggattcca tgaagtcctg cgcgtcgaag tgcgagcgag tgggatcggg ggaacctgcg
     1621 gcgtgcacga tggccaattg atttttgtga aagaggggct cgaggggcgc gaggctggga
     1681 tgcaatccaa aatatccatc gagatcgatg gaggcttctc ctactactcc acggcgcggc
     1741 tgagggattg cgatggtggg gcgcaggcgg tagtaattcg gctcggcgaa aggcactacg
     1801 atatttaatc cgtcggccgc gcctcgttga aacaggacta cgagctgttt cttgttgggc
     1861 atgggtgtgg cagcgacggc gcgttgcaag aacgcgggca tggcgctcag gcctaccatg
     1921 gccacgccac cttgcttcaa aaaatatcgc cgggaaaatc tcatgtgcga ctccttctgc
     1981 tacaacacca actcaacggc aactcgatat caactcgaca tcaaaccgaa gccaataagg
     2041 cgcggttagc gtctctgaaa ttctggagcg ccgagaacca gtccggtcac tactccgagg
     2101 tcaacttttt ttactggatc atcgagcttg gcttgcagg
//
LOCUS       DATCBB010000475         1086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_275320, whole genome shotgun sequence.
ACCESSION   DATCBB010000475 DATCBB010000000
VERSION     DATCBB010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275320"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..979
                     /locus_tag="VII37_05265"
     CDS             89..979
                     /locus_tag="VII37_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069169.1"
                     /translation="MNKYTSTYYPRRARWYGRIFYWGTAIRNRIAMDRIRMPNEMTVG
                     GLIGSFVIPGLAVYLRGPRLWGQAALLACAFLFLFFIVWLGYPFGNYALGMLLSIHAT
                     GFVYYCGPYLLEKEFMFRIGFTIAVLIALGLMIYTPIRGAIQNHWLTPLRVKNHVVIV
                     EKFASARGIKPGDWVMYSLNEASTGDAHGEGGAVWVREGVGWGPVLAMAGDRVTFSTN
                     SFAVNGIEQPHRLHMPTSGELVIPEKQWFIWPDLGISGHGNAGEANISAVMLSLATVP
                     ESQFVGKPFKRWFWREQVLP"
BASE COUNT          219 a          293 c          314 g          260 t
ORIGIN      
        1 agtttaagat ttgcgagccg gaagaagtgg tgcgatacaa cattgaccag cggcattgac
       61 cggcgcgcaa atcttaaact ggcggcgcat gaacaaatac acctcgactt actacccgcg
      121 tcgcgcgcgg tggtatggcc gtatttttta ttggggaacc gccatccgta atcgaatcgc
      181 gatggatcgc atccgcatgc cgaacgaaat gacagtcggc ggactcatcg gcagttttgt
      241 cattcccggt ctggcggtgt atctgcgcgg cccgcgcctc tggggacagg cggcgctcct
      301 cgcctgcgcg tttctatttc tattcttcat cgtgtggctc ggttatccat tcggcaatta
      361 cgcgttgggc atgttgctct cgatccatgc cactggcttc gtttattatt gcggccccta
      421 cctgctggag aaagaattta tgttccgcat tggtttcacg attgcggtgt tgattgccct
      481 cggcctgatg atctacacgc cgatcagagg cgcgattcaa aatcactggc tgacaccgtt
      541 gcgcgtcaag aaccacgtgg tgatcgtgga aaagtttgct tccgcgcgtg gcatcaaacc
      601 gggcgactgg gtcatgtatt cgctcaacga agcttccacc ggcgacgcgc acggcgaagg
      661 tggcgcggtc tgggttcggg aaggtgtcgg ctggggtccg gtgctggcca tggccgggga
      721 ccgcgttacg ttctcgacaa attccttcgc ggtgaacgga atcgaacaac cacaccggct
      781 gcacatgcca accagcggcg aactggtcat accggaaaaa cagtggttta tctggccgga
      841 tcttgggatt tccggccatg gcaatgcggg cgaggcaaac atttccgccg tgatgttgag
      901 tctggcaacc gttccggaaa gtcaatttgt cggcaaaccc ttcaaacgct ggttctggcg
      961 cgaacaagtt ttaccatgaa ccagtctggc aatcttgaac tgggtgacgc atgagcaaag
     1021 tcttctcatc ctactaccca ccgcgagcgc ggtggtatgg ctggattttt tatctgggaa
     1081 tcgccg
//
LOCUS       DATCBB010000476         1277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_80117, whole genome shotgun sequence.
ACCESSION   DATCBB010000476 DATCBB010000000
VERSION     DATCBB010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80117"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /gene="ruvX"
                     /locus_tag="VII37_05270"
     CDS             <1..306
                     /gene="ruvX"
                     /locus_tag="VII37_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY5069170.1"
                     /translation="LPLRLDGSHGEMAEEASRFAERVRKQIGVPVEMVDERLTSWEAE
                     RMLEEQGGRTMNPPTRAVSGRDSGRDSCKQPRKPKGEGKLTVDALAAAVMLKDYLER"
     gene            365..>1277
                     /gene="mltG"
                     /locus_tag="VII37_05275"
     CDS             365..>1277
                     /gene="mltG"
                     /locus_tag="VII37_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY5069171.1"
                     /translation="MAKFLLLILLIAAAGAAWLWYSVHQPYRNFPAEGVFVNVPHGSS
                     SRSVARLLRRQGVIRSAIAFEFYARRHPRRSLQAGEYFFDSAGSAHDVFWKLANGQVF
                     EQPFTVREGETMFDIARDLETAKFMPAAEFLRVAEDPALIRDLAPRATTLEGFLFPAT
                     YHLPRHPTAATLTADMVRKFKEEWSIVTASNPAEAAGSNQADATALSSTGLTNSRPIS
                     SIVTLASLVERETPKAEERPLVAGVFENRLHKSMPLQCDPTVIYALEQKDSYKGTLTL
                     ADLRIDSPYNTYVHTGLPPGPIGNPGEA"
BASE COUNT          267 a          384 c          376 g          250 t
ORIGIN      
        1 ctgccgctgc ggctcgacgg ctctcacgga gaaatggccg aagaagcgtc gcgttttgcc
       61 gagcgcgtgc gcaagcagat tggcgtgccg gtggaaatgg tggacgagcg gctgaccagt
      121 tgggaagccg aacggatgct cgaagagcag ggcgggcgga cgatgaatcc tcccacgcgc
      181 gcggtttcgg gcagagattc gggccgggat tcctgcaaac aaccgcgcaa gcccaaaggg
      241 gaaggtaaat tgaccgtcga tgccctggcc gccgcggtga tgttgaaaga ttatctggaa
      301 cggtagcttt cgcgacggta aagcatctaa caaagtagga ttcaactccg gagattcagc
      361 cgacgtggcg aaatttttac tcttgattct gttgattgct gcggccgggg ccgcgtggct
      421 gtggtactcc gtccatcagc cctaccggaa tttcccagct gagggagttt ttgtgaacgt
      481 gccccatggc tcatcgagcc gctcggtggc gcgcctgctg cggcgtcagg gcgtcatccg
      541 cagcgccatc gcgttcgaat tttacgcccg caggcatccg cgccggtcgc tgcaggccgg
      601 agaatatttt tttgatagcg ccggctcggc tcacgatgtt ttctggaagc tggccaacgg
      661 ccaggtattc gaacagccgt tcacggttcg cgaaggcgaa accatgtttg atatcgcgcg
      721 cgacctggaa accgcaaagt ttatgcccgc cgcggaattc ctgcgcgtcg ccgaagatcc
      781 cgcgctgatt cgagatctgg cgccgagagc cacgacactg gaaggatttc ttttccccgc
      841 gacgtatcat ttgccgcgcc atcctacagc cgccaccctc accgccgaca tggtcagaaa
      901 attcaaagag gaatggagca tcgtcactgc ctctaatcct gcggaagccg ccggctcaaa
      961 tcaggctgac gcaactgctt tgagcagcac gggtttgacg aacagccgcc cgatctcttc
     1021 catcgtcaca ctggcttcgc tggtggaacg tgaaacgcct aaagccgaag aacgcccgct
     1081 ggtcgcaggc gtttttgaaa accgcctgca caaatccatg ccgctgcagt gcgatcccac
     1141 cgtaatttac gcgctggagc aaaaggacag ctacaaaggc acgctgacgc tcgccgatct
     1201 gcgcatcgat tcgccttaca acacttacgt gcacaccggc ctgcctcccg gacccatcgg
     1261 caatcccggc gaagcgt
//
LOCUS       DATCBB010000477         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_372150, whole genome shotgun sequence.
ACCESSION   DATCBB010000477 DATCBB010000000
VERSION     DATCBB010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372150"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VII37_05280"
     CDS             complement(<1..276)
                     /locus_tag="VII37_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069172.1"
                     /translation="MDYAAAVTYLLSLGRELAAPTQAAAAKFNLENITTLEERLGHPS
                     RAYPSAHIAGTNGKGSTAAFLESILRHARLRTGLNTSPHLEKINERIR"
     gene            complement(289..1137)
                     /gene="accD"
                     /locus_tag="VII37_05285"
     CDS             complement(289..1137)
                     /gene="accD"
                     /locus_tag="VII37_05285"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY5069173.1"
                     /translation="MTWFKRDKKAIEQTTPPEERRVRTEGLWVKCDSCRTIVFRKDLE
                     ENLFVCPKCQFHFKMDAKKRLEMLLDGRYTEHDAGMTSSDPLNFTDTKPYATRLKEAR
                     KKLGMNDAIITAEGQLNGRPVVICAMEFGFIGGSMGAVVGEKVTRGIELAIETRRPFI
                     AVSCSGGARMMEGTISLMQLAKVSAALGKLDDARLPYISLLTDPTTGGVTASFAMLGD
                     LNIAEPGALIGFAGPRVIEQTIGQKLPEGFQRSEFLLEHGFLDAIVPRKELKSFIGTS
                     LDLLLN"
     gene            complement(1156..1692)
                     /gene="cobO"
                     /locus_tag="VII37_05290"
     CDS             complement(1156..1692)
                     /gene="cobO"
                     /locus_tag="VII37_05290"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY5069174.1"
                     /translation="MPEEKGLLIVYTGPGKGKTTCALGTAFRAVGQGLRVLMVQFIKG
                     SWHYGELDAAKMLGDDKFEIRPMGRGFVKIGGAETDPEDIKLAQECWEAGREAIYGGK
                     YDLVILDEINYTISYKMLDPDEVARALKDRPERVHVICTGRNAHPHITELADLVTEMR
                     EVKHPYTKGILAQRGIDY"
     gene            complement(1802..2245)
                     /locus_tag="VII37_05295"
     CDS             complement(1802..2245)
                     /locus_tag="VII37_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069175.1"
                     /translation="MSIRIIADSNQPSAAIEIPLDKPLPDYDLDQLEQPTPRDVDSIL
                     VSQGFRDLVDDARGVLMDLLVHPPFQAGAPENAKLDFTHSKPPALELVQLTGAICPGD
                     DEVYRPGLWIVLSDPHAKTKTAMPAATQDRIHCIAEELVKRLQLL"
     gene            complement(2271..2699)
                     /locus_tag="VII37_05300"
     CDS             complement(2271..2699)
                     /locus_tag="VII37_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY5069176.1"
                     /translation="MRLKLVMLGKTRRPELRIIIDDYVKRISRYAPIEVIELRESEAA
                     LKKFAFDQPSTIVLLDDAGKSLASEAFAKWLGEHRDRSTRLLIFVCGGAEGFPEDMRR
                     QAQQKLSLSAMTYSHELARVMLAEQLYRAFAILAGSAYPK"
     gene            complement(2711..>3164)
                     /gene="rsfS"
                     /locus_tag="VII37_05305"
     CDS             complement(2711..>3164)
                     /gene="rsfS"
                     /locus_tag="VII37_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY5069177.1"
                     /translation="TKVIPVKLESFPKGVQLAVHAAQEKKAGAITVLDLSGLGAFTEY
                     FVICTGFSTPQVQAICSEVEEQLYKQAQRSPEHREGRRSAEWALLDFGGFVVHVFSEQ
                     ARRYYDLERLWRSAPKLEIPDSPESLGHDSVTRRRSCDPEHFPGEAVS"
BASE COUNT          673 a          853 c          908 g          730 t
ORIGIN      
        1 acggatgcgt tcgtttattt tttcgagatg aggagaagtg ttgaggccgg tgcgcagacg
       61 ggcgtgccgc aaaatcgact ccagaaacgc ggccgtcgat cctttgccgt tcgtgccggc
      121 aatatgtgcg ctgggatacg cgcggctggg atgacctaga cgttcctcaa gggtcgttat
      181 attttcaaga ttgaatttcg ccgcggcagc ttgcgtgggt gcggccaatt cccggccgag
      241 ggaaagcagg taagttaccg cggcggcgta atccattttc tgcggagttc agttcagcag
      301 gagatcgagc gacgtgccga tgaaggactt cagttctttg cgcggtacga tggcgtcaag
      361 aaacccatgt tccagcaaaa attccgagcg ctggaatccc tcgggcaatt tctggccgat
      421 ggtttgttca atgacacgcg ggccggcgaa accgatcaat gcgccgggct cggcaatatt
      481 caagtcaccg agcatcgcga agctggcggt aacgccgccg gtagtcggat cggtgaggag
      541 agagatataa ggcagcctgg catcatcgag cttgccgagc gctgcggaga cttttgcgag
      601 ctgcatcaaa ctgatcgttc cttccatcat gcgcgcgcca ccggaacagg aaacagcaat
      661 aaacggtcga cgcgtttcga tggccagttc gatcccgcgg gtcacctttt ctccaacaac
      721 cgcgcccatg gaacccccga tgaagccaaa ctccatcgcg cagataacga cagggcggcc
      781 gttcaattgg ccttccgcgg tgattatcgc gtcgttcatt cctagttttt tgcgagcttc
      841 cttcaggcgc gtagcgtacg gcttggtgtc ggtaaaattc agcgggtcgg aggaagtcat
      901 gccggcgtca tgctcggtgt aacggccatc cagcaacatt tcgaggcgct tttttgcatc
      961 cattttgaaa tggaattggc acttcgggca gacaaaaaga ttttcttcca ggtctttgcg
     1021 gaaaacgatg gtgcggcagg aatcgcactt gacccacaaa ccttccgtgc gcacccggcg
     1081 ttcttcgggc ggagtggtct gttcgatggc ttttttgtcg cgcttaaacc aggtcataga
     1141 tctcagcgat gcggactaat agtcgattcc ccgctgcgcc agtataccct tcgtgtaggg
     1201 gtgtttcacc tcgcgcatct cggtgaccag gtccgccagt tcggtaatat gtggatgcgc
     1261 gtttctgccc gtgcagatta cgtgaacgcg ctcagggcga tcttttaatg ccctcgcaac
     1321 ttcgtccggg tcgagcattt tgtaactgat ggtgtaattg atttcgtcga ggatcaccag
     1381 gtcgtatttg ccgccgtaaa tggcttcgcg accggcctcc caacactcct gggccagctt
     1441 aatgtcttca ggatccgttt ccgcgccacc gatttttacg aaaccgcgcc ccatggggcg
     1501 aatttcaaat ttgtcgtcgc ccagcatttt ggcggcgtcc agctcgccgt aatgccagga
     1561 gcctttgatg aattgcacca tcaaaacgcg caggccttgt cctaccgcac gaaacgccgt
     1621 gcccagcgca caggtggtct tacccttgcc gggtccggtg taaacgatga gcagtccttt
     1681 ttcttccggc atcagattcc gatcaacggt gtagcgtgat tggcgaagcg caaagaagtg
     1741 tgcggattga gtgcggaaag gtcaagagct acacggccat taacgctgaa aaagtttctg
     1801 gctataacaa ttggagacgt tttacaagtt cctcggcgat gcaatgaatg cggtcttgcg
     1861 tggctgcagg catggcggtt tttgtttttg catgcgggtc ggacaaaaca atccacaagc
     1921 ccggacggta aacttcatcg tcgccgggac aaatcgcgcc agtcagctga acaagttcga
     1981 gcgccggagg cttggaatgc gtgaaatcga gcttcgcatt ttcgggagcg cctgcctgaa
     2041 aaggcgggtg caccagcaga tccattaaca cgccgcgagc gtcatcgacc agatcgcgaa
     2101 atccctggga aactagaatg gagtcgacgt cgcgtggcgt cggctgctcc agttgatcga
     2161 ggtcgtaatc cggcaaaggt ttgtccagcg gaatttcgat ggcggctgag ggctgatttg
     2221 aatcggcgat aatgcgaatg gacatagttg ctgctttcaa ctgcaaaaaa ctactttggg
     2281 taggcgctgc cggcgagaat tgcaaaagca cggtagagct gctctgcgag cataacacgc
     2341 gcaagttcgt gcgagtaggt catggcgcta agcgataact tttgctgcgc ctgccgccgc
     2401 atgtcttcgg ggaagccttc ggcgccgcca cacacgaaga tcaatagacg ggtgctgcga
     2461 tcgcgatgct cgccgagcca ttttgcaaaa gcttcagagg ccaggctttt tcccgcatcg
     2521 tccagcagca cgatggttga tggctggtcg aaggcaaact tttttagcgc cgcttccgat
     2581 tcccgcaact caataacttc gatgggcgca taacggctga tgcgcttcac gtaatcgtca
     2641 ataatgattc gaagctcggg ccggcgagtc ttgccaagca tgaccagctt caggcgcatt
     2701 ttttaggcca tcaagaaact gcttcgcccg ggaaatgttc cggatcgcag ctgcggcggc
     2761 gggtcacgct gtcgtggccc aggctttccg gtgaatccgg gatttcgagt ttgggtgcgg
     2821 aacgccagag ccgctcgaga tcgtaatagc gccgggcctg ttcgctgaag acgtgtacga
     2881 cgaatcctcc aaaatccaga agagcccatt ccgcggagcg ccgcccttcg cggtgctccg
     2941 gcgaacgctg cgcctgtttg tacaattgct cctcgacctc gctacaaatg gcctgcacct
     3001 gtggagtgct gaagccggta cagatcacga agtactccgt gaacgcgccc agaccgctca
     3061 aatcaagaac ggtaatggcc ccggcttttt tttcctgcgc cgcatgaacg gccagttgca
     3121 caccttttgg aaaggattct agtttcactg gtataacctt tgtt
//
LOCUS       DATCBB010000478         1112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_307582, whole genome shotgun sequence.
ACCESSION   DATCBB010000478 DATCBB010000000
VERSION     DATCBB010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307582"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /gene="atpA"
                     /locus_tag="VII37_05310"
     CDS             <1..947
                     /gene="atpA"
                     /locus_tag="VII37_05310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY5069178.1"
                     /translation="QKASSVVNVVRKLEEHGALGYTIVVVATASDSAAMQFLAPYAGC
                     TMGEYFRDRGEDALIVYDDLTKQAWAYRQISLLLRRPPGREAYPGDVFYLHSRLLERA
                     ARVNAVYVEAFTKGAVKGKTGSLTALPIIETQAGDVSAFVPTNVISITDGQIFLEADL
                     FNAGIRPAINAGISVSRVGGAAQTKVIKKLGGGVRLALAQYRELAAFAQFASDLDEAT
                     RKQLERGRMVTELMKQLQYAPLSVAEMAVTLFSVNKGYFDDVPVVKALSFESALHAQL
                     KANNKSLMDAIESSKDLSADNEKILSAAIEKFKTTAVY"
BASE COUNT          259 a          294 c          326 g          233 t
ORIGIN      
        1 ggcagaaagc ctcgtcggtg gtcaacgtgg tgcgcaagct ggaagagcac ggcgcgctgg
       61 gttacaccat tgtggtcgtc gcgaccgctt cggattcggc cgcaatgcaa ttcctggcgc
      121 cctatgccgg ttgtaccatg ggcgaatatt tccgcgatcg cggcgaagac gcgctgatcg
      181 tttatgacga tttaaccaag caagcttggg cataccgcca aatctccttg ttgctgcgcc
      241 gtccgcccgg tcgcgaagcc tatcccggcg acgtgttcta tctgcactcc cgtttgctgg
      301 agcgcgctgc gcgcgtgaat gccgtttatg ttgaagcgtt caccaaaggc gcggtcaagg
      361 gcaagaccgg ttcgctgact gcgttaccta tcattgaaac tcaggcgggc gacgtatccg
      421 ccttcgtgcc gaccaacgtg atttcgatca ccgacggcca aatctttttg gaagccgact
      481 tgttcaatgc cggtatccgt cctgcgatca acgccggtat ttcggtatcc cgcgtgggcg
      541 gtgcagcgca aaccaaggtc atcaagaagc tgggcggcgg tgtgcgtctg gcattggcgc
      601 agtaccgtga attggcggcc ttcgcacagt ttgcttcgga tctggatgag gcgacacgca
      661 agcaactgga acgtggtcgc atggttaccg agttgatgaa gcagttgcaa tatgcgcctt
      721 tgtcggtggc agaaatggct gtcacgctgt tctcggttaa caagggctac ttcgacgacg
      781 tgccagtagt caaagcgctg tcgtttgagt cagcgctgca tgctcagctg aaagccaata
      841 acaagagttt gatggacgcg atcgaatcca gcaaggatct gtccgccgac aatgaaaaaa
      901 tattgtcagc agcgatcgag aaattcaaaa cgacagctgt ttactaagcg gagcgaataa
      961 tggccggcag taaagagata cgcaccaaga tcaagagtgt agaaaacaca cgcaagatta
     1021 cgcgcgcgat ggaaatggtc gcagcagcca agatgcgtaa agcgcaggaa cgcatgcgcg
     1081 cagcccgccc ctatgcagaa aaaattcgcg ca
//
LOCUS       DATCBB010000479         1073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_469831, whole genome shotgun sequence.
ACCESSION   DATCBB010000479 DATCBB010000000
VERSION     DATCBB010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469831"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VII37_05315"
     CDS             <1..193
                     /locus_tag="VII37_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069179.1"
                     /translation="AHQGRQAASAARRSHISRARLAPLPALQTLPQAKKTARQKTPTT
                     GNSCFLIRTNKMIVLLFPT"
     gene            complement(273..>1073)
                     /locus_tag="VII37_05320"
     CDS             complement(273..>1073)
                     /locus_tag="VII37_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5069180.1"
                     /translation="KTMGIPLLHGRDFTDADGPDARPVMIVSAAIAKKFWGDEDPLGR
                     TLRKTGNPRIPYTIIGVVGDVRSTSLIEEFPSLYYPMASRAWPSMDVVVRGEGPTPEA
                     LLPAIREKVHELDSELALANVRTMEQWLSNGAAQPRLNTVLLSVFAFVALLIASVGIY
                     GVLAYSVSQRTGEIGVRMALGATPRDALRLVIREGMIVVAIGIGAGLLGGLALGRAVS
                     SLVFGVPVRDPAVFIGVAATLGIVALAACTIPALRASRVDPIVALRYE"
BASE COUNT          243 a          409 c          238 g          183 t
ORIGIN      
        1 tgctcatcaa gggcgccaag ccgccagcgc cgcacgccgc agccacatca gccgcgcccg
       61 cttagcgccg ttgccagcgc tccagactct gccccaggca aaaaaaaccg cccgtcagaa
      121 aactccgacg accggcaact cttgctttct tatccgcacc aacaaaatga tcgtattgtt
      181 attccccaca taagttcggg atccagcctc ggtgaccttc gcccgacacc ccacggattt
      241 ttggacctaa ctgaatttcg tacggctcac ccttactcgt aacgtagcgc cacaatcgga
      301 tccactcgcg acgctcgcag tgcaggaatc gtgcaagctg cgagcgccac aatccccagc
      361 gtagccgcta ctccaatgaa taccgccggg tcccgcaccg gcaccccaaa caccaggctc
      421 gaaaccgcgc gcccgagcgc caaaccgccc agcaatcctg ctccgatccc aatcgctacc
      481 acgatcattc cttcgcgaat taccagacgc aaagcatcgc gaggcgtcgc ccccaacgcc
      541 atgcgcactc caatctcccc ggtgcgttga ctcaccgagt acgccaacac cccataaata
      601 ccaaccgacg caatcagcag cgccacaaac gcaaacacgc taagcagaac cgtattcagc
      661 cgcggctgcg ctgcgccgtt cgaaagccac tgctccatcg tccgcacatt tgccagcgcc
      721 agttccgaat caagttcatg caccttttcg cgaattgcag gcagcaacgc ttccggcgtg
      781 ggcccctcac cccgcaccac aacatccatc gacggccatg cccgcgaggc catcggataa
      841 tacagcgacg ggaactcctc gatcagagac gtgctcctca catccccaac caccccgatg
      901 atcgtgtacg ggatgcgcgg attccctgtc ttacgcagcg tccgccccaa cggatcctca
      961 tcgccccaaa atttctttgc tatggccgcg ctgacaatca tcaccggtcg cgcgtccggc
     1021 ccgtccgcat cagtaaagtc gcggccgtgc agcagcggta ttcccatcgt ctt
//
LOCUS       DATCBB010000480         1515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_15851, whole genome shotgun sequence.
ACCESSION   DATCBB010000480 DATCBB010000000
VERSION     DATCBB010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15851"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1515
                     /locus_tag="VII37_05325"
     CDS             <1..>1515
                     /locus_tag="VII37_05325"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY5069181.1"
                     /translation="TEDGIDSELWVYVAVEAPIKFAVLKVRNKSGCLRRLSATGYIEW
                     VLGELRPKSLMHVITEIGLNSGALLARNAYNEEFGERVAFFDVDNVDGATCSFSGDRT
                     EFIGRNGTLQNPAAMSLSRMSCRVGAALDPCGAIRVPFELANGEEREIVFRLGAARNG
                     DDTRQMVQRFRGSAAARGALEVVTNYWKHTLGAVQVETPDPSINVLANGWLVYQVMAC
                     RLWGRTAFYQSSGAFGFRDQLQDVMALVHTRSDLVRAHLLLCASRQFQEGDVQHWWHP
                     PQGRGVRTKCSDDFLWLPLVACRYVLSTGDAGVLDESIHFLEGRPFKPDEDSYYELPK
                     QSDETATLYEHCVLAIQRGLSFGEHGLPLMGTGDWNDGMNKVGALGKGESIWLGFFLH
                     QVLMEFENVAQLNGDAAFVELCRKETAQLRQNVEQHGWDGEYYRRAYFDDGTPLGSAS
                     NPECQIDSIAQSWSVLSGAASAERSRMAMDALDKHLVHREDAVIQLLTPPFDKST"
BASE COUNT          312 a          390 c          474 g          339 t
ORIGIN      
        1 acggaggatg gtatcgactc cgaactatgg gtttatgtgg cggtggaggc gccgatcaaa
       61 tttgcagtct tgaaggtgcg caacaagtca ggctgcttgc gccgcctttc cgccactgga
      121 tacatcgaat gggtgctggg agaactgcgg ccgaaatcgt tgatgcatgt gatcacggag
      181 atcggtctca acagcggcgc actgctggcg cgtaacgcat ataacgagga gttcggcgag
      241 cgagttgcat ttttcgatgt ggacaatgtg gacggggcga cttgcagctt cagtggcgac
      301 agaactgaat tcattggtcg caacgggacg ctacagaatc cggccgcgat gtcgttgtcg
      361 cggatgtcct gcagggtagg tgccgcactc gatccttgtg gtgcgatccg ggtgccattc
      421 gagctggcca atggtgaaga acgcgagatc gttttcaggc tgggggccgc tcgaaacggc
      481 gatgatacgc gccagatggt gcagcgtttt cgaggatctg cagccgcgcg tggcgcgctc
      541 gaagtggtaa cgaactactg gaagcacacc ctcggggcgg tacaggtgga aactcccgac
      601 ccatccatca atgtgctggc aaacggctgg ctcgtgtatc aagtcatggc ttgccgtctg
      661 tggggacgga ccgcgtttta ccaatcatcg ggcgcttttg gttttcgcga tcagctccag
      721 gacgtaatgg cactcgttca cactaggtca gaccttgtgc gcgcgcattt gctcctctgc
      781 gcctcccgcc agttccagga aggcgatgtt cagcattggt ggcatccgcc gcagggtcgt
      841 ggtgttcgta cgaagtgttc ggatgatttc ctttggctgc cgttggtagc gtgtcgctat
      901 gtcttgagta ctggtgatgc gggcgtgctg gatgagtcaa tccattttct ggaaggccgg
      961 ccgttcaagc cggacgaaga ttcgtattac gaactaccca aacaatctga cgaaactgcg
     1021 acgctttacg agcattgcgt gctagcgata caaaggggcc tcagctttgg cgaacatggc
     1081 ctgccactta tgggtaccgg cgactggaac gacggcatga ataaagttgg cgcgctaggt
     1141 aaaggcgaaa gcatctggct gggatttttc ctccatcagg tgctcatgga attcgaaaac
     1201 gttgcacagc tgaatggcga cgccgctttc gtcgaactct gtcggaagga aacggcacag
     1261 ctgcgccaga atgtcgagca gcatggctgg gacggtgaat attatcgccg cgcttacttc
     1321 gacgacggca cgccgcttgg atcggcaagc aaccccgaat gccagattga ttcgattgcg
     1381 caaagctggt ctgttctgtc cggcgcggct agtgccgagc gctcacggat ggccatggat
     1441 gcgctcgaca agcaccttgt gcaccgtgag gacgctgtga tccagttact gacgccgcca
     1501 ttcgacaaat caact
//
LOCUS       DATCBB010000481         1055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_372255, whole genome shotgun sequence.
ACCESSION   DATCBB010000481 DATCBB010000000
VERSION     DATCBB010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372255"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VII37_05330"
     CDS             <1..292
                     /locus_tag="VII37_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069182.1"
                     /translation="IAELSLKQGRILAHATEMVRPGGILLYSTCSLERDENEAVAAKF
                     VKEHAGFVQLRLANARILDAAADLATASGAIRTWPHRHDVDGFFMIAFQRPV"
     gene            388..>1055
                     /locus_tag="VII37_05335"
     CDS             388..>1055
                     /locus_tag="VII37_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY5069183.1"
                     /translation="MSFITQALTALKRILIVVGIAIAFTLGLLGAIYLSLRSSETKVP
                     DIIGKDRTAAEDTISSAGLNFRVRATRPTSDAKPDTVLIQLPHAGEIVKVGQTVAVDI
                     SRPTKEGETSTTANSNTNTNTEKKPENVNATNSNTNSNDNKQKKKPANKNDNANANAN
                     RAANRNRNSNGNVNRATNGNLNANPAGTPVHSNLNSNGVPRGNTNRGAGNVNTNRRPP
                     VTAPT"
BASE COUNT          268 a          303 c          283 g          201 t
ORIGIN      
        1 tatcgccgag ctgtcgttga agcagggacg cattctcgcg catgccactg agatggtccg
       61 gccaggtgga atactcttat actcgacctg ctcgcttgaa agggacgaga acgaggccgt
      121 ggccgcgaag ttcgtgaaag agcatgccgg cttcgttcag ttgcggctag ccaacgcgcg
      181 gattctcgat gccgcagctg atctggccac ggcgagtggc gccattcgca cttggccgca
      241 tcgtcacgat gtggacggct ttttcatgat cgcgtttcaa cgcccggtgt agcgcagctc
      301 ggttgcggct gtttgcgcgc ggatgttaga cttcgccctg acctgcgccc ggattgattt
      361 gcgccgtgaa aagagactga atccatcgtg tctttcataa ctcaagcgct cactgcgcta
      421 aaacgaatct tgatcgtggt cggaattgcc atcgctttca ccctggggct gcttggtgct
      481 atttatctgt cgttgcggag ttccgagaca aaagtgcccg acatcatcgg caaggaccgg
      541 acggctgcgg aggacactat tagctcagca ggtttgaatt ttcgcgtgcg cgccacgcgg
      601 ccaaccagcg acgcaaaacc tgatactgtt ctaattcaac tcccgcatgc cggagagatc
      661 gtcaaggttg gacaaactgt ggcggtggac atcagccggc ccacgaaaga aggcgagact
      721 tcaactactg cgaattcaaa caccaatacc aacaccgaaa agaagcccga aaacgtcaac
      781 gccactaatt ccaatacaaa ctcaaacgat aacaagcaga aaaagaaacc ggcgaacaag
      841 aatgacaacg cgaatgcgaa cgcgaatcgc gcggccaacc gaaaccgtaa cagcaatggc
      901 aatgttaacc gcgcgaccaa tggcaacctg aacgctaatc cggccggaac tcccgttcac
      961 agcaacctga attcgaatgg cgtacccagg ggaaatacga accgcggcgc cggtaatgta
     1021 aatactaacc gccgtccgcc agtcaccgcg ccgac
//
LOCUS       DATCBB010000482         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_307726, whole genome shotgun sequence.
ACCESSION   DATCBB010000482 DATCBB010000000
VERSION     DATCBB010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307726"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1613
                     /locus_tag="VII37_05340"
     CDS             168..1613
                     /locus_tag="VII37_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5069184.1"
                     /translation="MKSRLSSTIWTLVVLNGIVLAAVIALAYNAGRQSGNVSLLDFGA
                     LPSAAAGAGAVAVGLALLVTIILAWRLGGGLLSPVGELAKFSERLAVGDPKARVEVTS
                     TNELGYIADNLNRAVAKVSKAANNQESSDALQRSITELLTVINQVARGDLSLRGKVTN
                     DALGNVADSVNYMLDNFTKVLERVRKAAMEVTACSNNILVAADQMQAGATQQDQEITN
                     TSSAVEELTVSMKQVSNNAEASAEAARRALDAAEQGNRAVRDTLEGMQRIRSSVQATA
                     KKIKSLGDRSLEISEIINVINDITEQTNLLALNAAIEAARAGEAGRGFAVVADEVRKL
                     AEHSRSATKDIAALIKAIQAETNEAVVVMEEGTKEVESGATLADQAGRALDAISNVVR
                     QSAELVQEISLASKQQVRGTEGVAHAMQIISNITRQTSQGTRGTVATVSQLVKLSDQL
                     NDGLAQFRASSKQGGGTHQEESERVPVGVSR"
     gene            1750..>2363
                     /locus_tag="VII37_05345"
     CDS             1750..>2363
                     /locus_tag="VII37_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY5069185.1"
                     /translation="MSEIRMLIEERTGICFDKSRERFLVTRVREHLQARGFERGTDFL
                     RAARKTNVEYEALLERLLTQETTFFRYPAVYEAFQKRALPELHIKKFWKNPRTLRIWS
                     AGCSTGEEPYSIAITIADTLSFADAWNVEILATDVSRVALKHAENGLYSGRSISSVSE
                     AQLAAHFSPIKHRQQVKPRLHKMISFTQMNLASAVYVGRMDVIF"
BASE COUNT          551 a          636 c          675 g          501 t
ORIGIN      
        1 tgaaaatgtt ccacattgca tcggaatgct gcgacatgac gatcgtctcg cgttggtgat
       61 tgacttacgc aggcttttgg aagcttttcc gggaccaagt atttaggttt gtgtcggaat
      121 catccgtcga tggctgacgg ctcggtgaaa acgatcttgg aggaagtatg aaatcgcgat
      181 tgagttctac catctggacg ctggttgtgc tgaacggcat cgtgttggcc gccgtgatcg
      241 cactggccta taacgccggg cgtcaatccg gcaacgtgtc tcttctcgac ttcggcgctt
      301 tgcctagtgc agccgccggg gctggcgcgg ttgctgtagg cctcgctctg ctggtgacca
      361 tcatcctggc gtggcgactt ggcggagggc tgttgtcacc cgtcggagag ttggccaaat
      421 tttccgaacg gctggccgtc ggagatccca aggcgcgcgt ggaagtcact tcgaccaacg
      481 agctgggcta catcgctgac aatttgaatc gcgcggtagc taaggtttcg aaggccgcca
      541 ataatcaaga gtccagcgac gcattgcaac gcagcatcac ggaattgttg acggtcatca
      601 atcaggttgc gcgcggagat ttatcgctgc gcggcaaagt aaccaacgat gcgctgggca
      661 acgttgctga ttccgtgaac tacatgctcg acaacttcac caaggttctc gaacgcgtcc
      721 gcaaagcggc catggaagtt accgcgtgct ccaacaacat tctggtagct gcggatcaaa
      781 tgcaggccgg cgccactcaa caggatcagg aaattaccaa tacatcgagc gccgtggaag
      841 agcttaccgt ctccatgaaa caggtttcca acaacgcgga agctagcgcc gaagctgcgc
      901 gtcgtgctct agacgccgcc gagcaaggca accgtgccgt tcgcgacacg ctcgaaggca
      961 tgcaacgtat ccgttcgtcg gtgcaagcga ctgccaagaa aattaagtcg ctgggtgatc
     1021 gttcgctgga aatttcggaa atcatcaacg tcatcaacga catcaccgaa cagaccaact
     1081 tgttggcttt gaacgccgcc atcgaagccg cgcgcgctgg ggaagctggg cgcggctttg
     1141 cggtggttgc cgacgaagtt cgcaagctgg ccgagcatag ccgcagcgcc acgaaagaca
     1201 tcgccgcgtt aattaaagcc attcaggccg agaccaacga agcggtggtg gtcatggaag
     1261 aaggcaccaa ggaagtcgaa agcggcgcaa ccctcgccga tcaggccggc cgagcgctcg
     1321 atgccatctc caacgtcgtg cgtcaatctg ctgaacttgt acaggaaatt tctttggctt
     1381 ccaaacaaca ggtgcgcggg acagaaggcg tcgcacacgc catgcaaatc atctccaaca
     1441 ttacgcgcca aacttcgcaa gggacgcgtg gaacggtggc aacggtcagc cagctcgtaa
     1501 agctgtcgga tcagttaaac gacggtcttg cgcaattccg cgcctcttct aaacagggcg
     1561 gtggcactca tcaggaagaa tctgagcgcg ttccagtagg agtgtcgcgg taaaaattcc
     1621 tgaacgacgt agtgcatgag ttgctaaacg atttttagct ttggcaaatg ccgaagccga
     1681 acggaataac caagcgtgag agtcgcgaac gagcgcccga taacagagct cgaacttagt
     1741 gagcacgagt tgagcgagat ccgcatgcta attgaagagc gtacggggat ttgtttcgat
     1801 aaatcgcgcg agcgattcct ggttacccgt gtgcgtgaac acctgcaagc tcgtggcttc
     1861 gaacggggca ccgatttcct ccgcgcggct cgcaaaacca acgtggaata cgaagcgctt
     1921 ctggaacgcc tgcttaccca ggagacaacc ttctttcgtt acccggcagt ttatgaggct
     1981 tttcaaaagc gtgctttgcc agaattgcat ataaaaaagt tttggaagaa tccgcgtacg
     2041 ttgcgcatct ggagcgccgg ctgttctacc ggcgaggagc cttactccat cgccatcacc
     2101 atcgcggaca ccttgtcatt cgcagacgcc tggaatgtgg agattctcgc cacggacgtc
     2161 agccgtgtgg cgctcaagca cgctgaaaat ggcttgtatt caggacgaag catttcttct
     2221 gtcagcgagg cgcagttggc cgcacacttc agtcccatca agcatcggca gcaggtcaag
     2281 ccgcggctgc acaagatgat cagttttacg caaatgaatc tggcttccgc tgtgtacgtt
     2341 gggcgcatgg atgtaatttt ctg
//
LOCUS       DATCBB010000483         1167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_241988, whole genome shotgun sequence.
ACCESSION   DATCBB010000483 DATCBB010000000
VERSION     DATCBB010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241988"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VII37_05350"
     CDS             complement(<1..482)
                     /locus_tag="VII37_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5069186.1"
                     /translation="MSLETARRVLKIEAQAIQDVLARLDERFVRAVDVLFGCKGRVVV
                     SGMGKSGLIGRKISATFSSTGTPSFFLHPAEALHGDLGMLARGDALLAVSYGGETEEI
                     VRLLPALKRLEMALVTLTGKTNSTLAEASDVALDVSVAEEACSLNLAPTASTTVAMAV
                     G"
     gene            complement(479..>1167)
                     /gene="kdsA"
                     /locus_tag="VII37_05355"
     CDS             complement(479..>1167)
                     /gene="kdsA"
                     /locus_tag="VII37_05355"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR01362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY5069187.1"
                     /translation="PYIFKASYDKANRSSVKSFRGPGVKEGLRVLGKIKKDFGVPVLT
                     DIHESGQAAAAAEVCDVLQIPAFLSRQTDLLVAAAKTGRIVNVKKAQFLSPWDMSNVV
                     EKIASAGNEKIILTERGASFGYNNLVVDMRAFPVMRKFGYPVVYDVTHSLQLPGGEGK
                     TSGGQPEYIETLALAGVAAGVDGIFLETHDNPGAALSDGANALPLAQLASLLARVKQV
                     AELVRSWGTR"
BASE COUNT          234 a          459 c          259 g          215 t
ORIGIN      
        1 cccaccgcca tcgctacggt cgtgctcgcc gtcggagcca gattcagcga acacgcctcc
       61 tcggctacac tcacatccaa cgccacgtcg ctcgcctccg ccagcgtcga gttcgttttc
      121 cccgtcaacg tcaccagcgc catctccaag cgcttcagcg ccggcagcag ccgcacaatt
      181 tcttccgtct ctcccccgta agacaccgcc agcagcgcat ccccgcgcgc cagcattccc
      241 aaatctccgt gcagcgcttc cgcgggatgc aaaaaaaacg atggcgtgcc ggtgctcgaa
      301 aacgtcgccg aaatcttccg cccgatcaac cccgacttgc ccatcccgct caccaccacg
      361 cgccctttgc agccgaacaa cacatccacg gcgcgcacaa acctctcgtc cagccgcgcc
      421 agcacatcct gaatcgcctg cgcctcgatt ttcagcaccc gccgcgcggt ttccaggctc
      481 atcgcgttcc ccaactccgc accagctccg ccacttgttt cacccgagcc agcagggaag
      541 ccagctgcgc cagcggcaaa gcgttggcgc catccgaaag cgccgcgccc ggattgtcat
      601 gcgtctccag aaaaattcca tcgacacctg ctgccactcc cgcgagcgcc aaagtctcga
      661 tatattccgg ctgcccgccg ctcgttttgc cttcgccgcc cggcaattgc agcgaatgcg
      721 tcacgtcata caccaccgga tacccgaatt tccgcatgac cggaaacgcg cgcatatcca
      781 ccaccaggtt gttgtagcca aacgacgccc cgcgctccgt caaaataatc ttctcattcc
      841 ccgcgctcgc gattttctcc accacgttgc tcatatccca gggagaaaga aactgcgcct
      901 tcttcacatt gacaatccgc ccagtcttcg ccgccgccac cagcaggtct gtctgccgcg
      961 acaaaaacgc cggaatctgc agcacgtcgc acacttccgc cgccgcggcc gcctgtccgc
     1021 tctcgtgaat atccgtcagc accggcacgc caaaatcctt tttgattttc cccaggacgc
     1081 gcaggccctc tttcactccc ggcccgcgaa atgatttcac cgacgagcgg ttggccttgt
     1141 catacgacgc cttgaaaatg taaggca
//
LOCUS       DATCBB010000484         1254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_210218, whole genome shotgun sequence.
ACCESSION   DATCBB010000484 DATCBB010000000
VERSION     DATCBB010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210218"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..>1254
                     /locus_tag="VII37_05360"
     CDS             470..>1254
                     /locus_tag="VII37_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY5069188.1"
                     /translation="MASSVWKGYLSFGLLSIPIRLFTAARGDRIGLNQLHEVCHSRIK
                     MPLFCPTCDRKVERSEIVKGYEYEKDQYVLFSDEDLDKIEPESARAMEILEFVKVDEI
                     DPLYYEASYYLTPEEEGKKAYSLLLKAMEDSGYAAIAKVTMHQREHIVVIRARAEGMT
                     LHTMFYQSEIRAVEEFSHQDKVEPKEQEKKLAMQLIDSLAATFEPAKYRDEYQESMRA
                     MISAKQKGQEVTATVHPQRAPVIDLMDALRKSINAKPAASGKKP"
BASE COUNT          305 a          319 c          388 g          242 t
ORIGIN      
        1 cttggaatcc cactgggccc gatcaacgga cattgcggag ttatgagaaa gtctttggca
       61 gcccgtcgcg gtgagaatga cggccacgag aatcaaatta agaagtttgc gatgcataac
      121 tctcccatcc ggacccatac ggtaaccacg ccggggcaaa gaaaacggga catacggacg
      181 ttcgcggtta tcgcactgtc ttgatcccga ctagctttga acgatggagc atgattttct
      241 tgcgaggact ggcggggatc gcccgcgagt accgcccttg cggtttaggt agacaccggg
      301 tgacgggcag tggttccgca gcccggatgt tttgcactat tggaaaccca aagccaatgt
      361 tgtgttttgc acggctttcg ccttcaattc gccttgcgca gcctgcgggt ggcgattaac
      421 cttgagcgca agcttgcagc ggcacaatag cgtgccctaa ggagcagcga tggcgtcctc
      481 ggtctggaaa ggctatttgt cgtttgggct cctttcgatt cctattcgtc tgttcacggc
      541 ggcgcgcggc gaccgcatcg gattgaacca actgcatgaa gtttgtcact cgcggatcaa
      601 gatgccgctt ttttgcccca cctgcgaccg caaagtggaa cgctcggaaa tagtcaaggg
      661 ctacgagtat gagaaggatc agtacgtgct gttcagcgac gaagacctgg acaagatcga
      721 gccggagtca gcgcgggcga tggagattct ggaattcgtg aaagtggatg aaatcgatcc
      781 gctgtattac gaagcttcgt actatctgac gccggaagaa gaaggcaaga aagcgtactc
      841 gctgctgctg aaagcaatgg aggattccgg gtacgcggcg atagcgaagg tgacgatgca
      901 ccagcgcgag cacatcgtgg tgattcgcgc gcgcgccgag ggcatgacgc tgcacaccat
      961 gttttaccag agtgaaatcc gcgcggtgga ggagttcagt caccaggata aggtggaacc
     1021 caaggaacag gaaaagaagc tggcgatgca actgatcgac agcctggcgg cgacgtttga
     1081 gccggcgaaa tatcgcgatg agtaccagga aagcatgcgg gccatgatct ccgcgaaaca
     1141 aaagggccag gaagtgacgg ccacggtgca cccgcaacga gcgccggtga tcgacttgat
     1201 ggacgcgctg agaaagagca tcaatgccaa gccggcggcc agcggcaaaa agcc
//
LOCUS       DATCBB010000485         1910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_80511, whole genome shotgun sequence.
ACCESSION   DATCBB010000485 DATCBB010000000
VERSION     DATCBB010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80511"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VII37_05365"
     CDS             complement(<1..431)
                     /locus_tag="VII37_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069189.1"
                     /translation="MSERNFTLSIAAQPLTRLTEFDPEDEYRPEPEPEALPPAWLDGS
                     TRIGIHTSIAGGHRNALETARKLGCNALQIFSANPRQWQGGSARIPEVDALAFRARRA
                     ELGLGPLVIHANYLINLASPQPMLRVRSIQAFHDEIVRGMSL"
     gene            733..1098
                     /locus_tag="VII37_05370"
     CDS             733..1098
                     /locus_tag="VII37_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069190.1"
                     /translation="MLGALLLITGALIALFHPVMLVSPHDEINGAVHIYAGYLAARNL
                     ALAIMLLAALGFAAKRLLNNLLLLVSLVQFLDALIDVGEGRLPVAIGVIILAVLFSAA
                     SASLSGYPFWQIRAWKETD"
BASE COUNT          404 a          586 c          471 g          449 t
ORIGIN      
        1 agcgacatcc cgcgcacaat ttcatcgtgg aacgcctgga tcgaccgcac cctcaacatc
       61 ggctgtggcg aggccaggtt aatcaaataa ttggcgtgaa tgaccagcgg cccaagcccc
      121 agttcggctc ggcgagcgcg aaaagccagc gcatccactt ccggaatgcg cgccgacccg
      181 ccctgccact gcctgggatt ggccgaaaat atctgcagcg cgttgcaccc cagcttgcgc
      241 gccgtttcca gcgcgttccg gtgcccgccc gcaatagacg tatgtatccc gatgcgagtg
      301 ctgccatcca gccacgccgg cggcaacgcc tccggctcag gttccggccg gtactcgtct
      361 tcgggatcaa actcggtaag ccgcgttagg ggttgtgctg caatgctcaa tgtaaaattt
      421 cgctcactca taaaccagca attataacac acgcgcgaaa acgggtccct cgactcgaaa
      481 tcctggatag tcggtcagtt tccggccgcc gcgcttttta aggcatatcc gaaaggtcat
      541 gtaatttaac tcctccgctt agcgtattca caaagaacct aaacccagca gttagtgcgg
      601 tgagtgcgcg ccacttctct ggatcggagc atacccgtgt ctttgtccca atctggtaac
      661 aagagcgttc aaacaaattc ctctctgctc cgcgagaagc ccgcaattgg ttggttccca
      721 aaagtggttg cggtgctcgg cgcgctgcta ttaatcacag gagcgctcat cgcgctgttc
      781 catccggtaa tgttggtttc tccgcacgac gaaattaatg gcgcggtcca catctacgcg
      841 ggttaccttg ctgctcgcaa cctggcgctg gccatcatgc tgctcgcggc gctgggcttc
      901 gccgcaaaac gcttgttgaa caatctgttg ctgctcgtct cactcgttca gttcctcgac
      961 gcccttatcg acgtcggcga aggccgctta cccgttgcca tcggagtcat aattcttgcc
     1021 gtgcttttct ccgccgcatc tgcttctctc tccggttatc cgttctggca gatccgcgct
     1081 tggaaagaga ccgattagca aaacgtcgaa gaaaccaagc gaaaccgcaa ttccggtgcc
     1141 cggtaagcgt gttcatcaag actcttacag ttcccgttaa attcgtttgc cttgagtcct
     1201 acgtttccgc ggagtaccct aaaatttgga gggcatatga ttagctcacc ttcactccca
     1261 gttatcatca cttgttcagg ctgcctcctc gcgttggtcg taggggttat tttccgcact
     1321 accacttttc ttgggtacac tgctcgactc ggatttctgc ccaaaggttt aacccgctgg
     1381 ttgtacggcg tgaagaacac caatccgacg aactgaccgg cagcgctctt ccccgccatt
     1441 tgattgagaa atgcgcggta cggaaacccc gcaatcgcat atgcgagaaa tccagttagg
     1501 acaagggcca actcgatggc tcctacaacc ttggccaatt tgatcttatt gggcgtcgga
     1561 ttttgcttaa agatccgaag tgctgactcg ggataggcga acaagagaag accatagccg
     1621 ccgaccaaaa cacctgctaa tgcggaacct aacaggtctc tgtgtgcctc tgtgagcgtg
     1681 atttttgaat attatatcaa ttcctttacc gccgtcgttt gccccgtctg cgcccgaaca
     1741 ggtaaactgc actgttcctc atcctagccc ggagccgtca tgctagtcgt aatgcagtct
     1801 cacgccaccg agccacagat tcgcgctgtt tgcgagcgca tcgaaagcct cggccttaag
     1861 gcgcatccca ttcccggcgc cctccgcacc gccatcggca tcaccggcaa
//
LOCUS       DATCBB010000486         4472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_80520, whole genome shotgun sequence.
ACCESSION   DATCBB010000486 DATCBB010000000
VERSION     DATCBB010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80520"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VII37_05375"
     CDS             <1..633
                     /locus_tag="VII37_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY5069191.1"
                     /translation="EAIIKYWPADVQPLPALEEFSSPDGPAAIGKRYNIWAKSTGAPY
                     AAAKGDLTSGEWTGLLFETIAEDKLIQPTILYDFPIDISPLSKQKPDDPSLVERFEIY
                     IGGMEIANGFSELNDPAEQERRFLAQIAEGGDESPKRLDVDYIQALCYGLPPTAGEGI
                     GIDRLTMLLTDSRSIRDVILFPLLRPEASPHQELAEPQSRQPTANPSEKA"
     gene            649..1875
                     /locus_tag="VII37_05380"
     CDS             649..1875
                     /locus_tag="VII37_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069192.1"
                     /translation="MRFERFVARRYLRSPHRPAVLRLVTLFSIIGVAAGVATLVIALA
                     MNTGFRETLQDRLLGVTAHISLTRPGSGGIRDYEALATKFSTLPGVRSVTPAVYQTVL
                     MTFAGQSRGVVTKGIDPERERRADEALQRIVAGQLDFSPDKDGIGALLIGKQLAEEWK
                     LSPGDYVTLMSPQGRLTPFGLLPRSRRFRIAGVFDSGFYDYDQNWCFLNLADAQNLSG
                     GGDAVNVLEFRLNNPENASSIAQQIQQIAGPGFAASTWMDENRALFRALRMEKLVTAI
                     FIGLITFVAGLNILVVLSMTVTDKARDIAVLMSLGTRRAQIRKIFLWQGITIGAVGTL
                     VGLCVGYLFAFIAGFYHLIPLDPQVYAVPYVPFHPGLLDGVWITVIAMAISFTATILP
                     ARAAASLLPVEILRYE"
     gene            2209..2997
                     /locus_tag="VII37_05385"
     CDS             2209..2997
                     /locus_tag="VII37_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5069193.1"
                     /translation="MTAADSNSTLSNVQVADAAAQLATVVLEGRHLKKSYGQGPKILP
                     VLVDANITLHKGEMIAIVAPSGAGKSTLLHLLAALDMPTSGTVYFATKAIESNQDSLL
                     AEFRNRAVGFLWQRHQLLLDFTAEENVAMPLLLRGENFASAQGMARKWLTEVGLESRA
                     GHRAGELSGGEQQRVSIARALVTGPAVLLADEPTGNLDEQNAWAVFELLVRLHRTLGL
                     TSLIATHNLALAARCDRVLTLEHGVLTARKATAAASATPGGEAS"
     gene            2999..>4472
                     /locus_tag="VII37_05390"
     CDS             2999..>4472
                     /locus_tag="VII37_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY5069194.1"
                     /translation="MFERYTEKARRVIFFARYEASQYGSPYIETEHLLLGLMREDKAL
                     ANRFLRQQGSIESIRKEIEARITIRERISTSVEVPLSAECKRILNMAGEEAERLGVKH
                     VGTEHLLLGILREEKCFGAEILMERGLRLSTLREELARTSGEKTTAARPKETSLLAEF
                     SRDLTQAASEGSLDPLVGRDGEVERVIQILCRRTKNNPVLIGEPGVGKTAIVEGLAQR
                     ISDGDVPPFLADKRILSLDLSLIVAGTKYRGQFEERLKTIMKELMESQNSIVFIDELH
                     TLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPGEYRKSVEKDRSLERRFQSVKVP
                     APNEEEAVQVISGVRERYEKFHAVSYTDEALKYSVYLSNRYIPDRFLPDKAIDLIDEA
                     GARVKLRQASVPEEVGEVQKRVKFITHRMETAIANHEFEKARFYSEEERKEKENLRGL
                     RERYKLDDASTGIVTREDIEEVVSRWTGIGVSSIKEDEQQK"
BASE COUNT          958 a         1259 c         1227 g         1028 t
ORIGIN      
        1 gaagccatta ttaaatattg gcccgcggac gtgcagccgc ttccagcgct ggaagaattt
       61 tcgtcacccg atggcccggc cgccatcggc aagcgctata acatctgggc caaaagcacc
      121 ggcgctccct atgctgcggc aaagggtgat ttgacctctg gtgaatggac cggcctgtta
      181 ttcgaaacca ttgccgaaga caaattaata cagcccacaa ttctttacga tttccccatc
      241 gacatttccc cgctatcgaa acaaaaaccc gacgacccct cgctagtgga gcgctttgag
      301 atttacatcg gcgggatgga aatcgccaac ggcttttcgg agttaaacga ccctgccgaa
      361 caggagcgcc gtttcctggc ccaaattgcc gaaggcggcg acgaatctcc aaagcgcctg
      421 gatgtcgatt acattcaggc tctttgctat gggctgccgc ccaccgcggg cgaaggtatc
      481 ggcatagacc gccttaccat gctgctgacc gattccaggt ccattcgcga cgtcatttta
      541 tttcccttac tacgtccgga agcttcaccg catcaggaac ttgcagaacc tcaatcgcga
      601 cagccgaccg cgaatcccag cgagaaagcg taaccagcag ggccttccat gcgctttgaa
      661 cggtttgtag cccggcgtta tctgcggtca ccgcaccgtc ccgcggtact gcgcctggtt
      721 actttgtttt cgatcatcgg cgtggccgcg ggcgtggcta ctttggtcat cgccttggcg
      781 atgaacaccg gcttccgaga aacgctccag gatcggctgc ttggcgtgac tgctcacatt
      841 tcgctcacgc gcccaggctc cggcggcatt cgcgactatg aagcgctcgc caccaaattt
      901 tcgaccctgc ctggagtgcg ctcagttact ccagcggtat atcaaacggt gctcatgact
      961 ttcgccggcc aatcccgtgg ggtggttacc aaaggcatcg accccgaacg agagcgtcgc
     1021 gcggatgaag cgctccagcg gatcgttgca ggtcaactcg atttctctcc agataaagac
     1081 ggtattggag cgctgctcat cggaaagcag ctcgccgaag aatggaaact ctcgccaggc
     1141 gattacgtga cgctcatgag ccctcagggc cgtttgactc cgtttggact gcttccacgt
     1201 tcgcgaagat ttcgcatcgc cggcgttttc gattccggct tttatgatta cgatcaaaat
     1261 tggtgtttcc tcaaccttgc tgatgcgcag aatctttccg gcggcgggga cgcggtgaat
     1321 gttctggaat ttcgcctgaa taatcccgag aacgcctcga gtatcgcgca acagattcag
     1381 caaattgccg gtcccggttt tgcggcttcg acctggatgg acgagaaccg cgccttgttc
     1441 cgggctttgc gaatggaaaa attggttacc gctattttca ttgggcttat tacttttgtg
     1501 gctggtctta acatactggt ggtgctttcc atgaccgtaa ctgacaaagc tcgcgatatc
     1561 gccgtcctga tgtcgctggg cacccgtcgc gcgcagattc gaaagatttt tctgtggcag
     1621 ggaattacga tcggcgctgt gggaactctt gttggtctgt gtgtgggcta tctgttcgct
     1681 tttatcgccg gtttttacca cttaattccg cttgatcccc aggtttatgc cgtgccgtat
     1741 gttccttttc atccaggatt attggatggc gtgtggatta ccgtcatcgc catggctatc
     1801 tcgtttactg ccaccatctt gccggcgcgc gccgccgcga gcctcctccc tgtggagatc
     1861 ctgcgatacg agtgacccgt atgtacgatt ggtgcgcaat ttgctccatg tagttgtgca
     1921 gaggccaggc gggttttcac cggcgcaacc ctcgagccgg tgagtcaaga gccctcctca
     1981 accagttgaa acaaaggatg ttagcggtgc aacaaaatgg cacaccagcg cccattttcg
     2041 cgagcgaccc acgtcgggca aaagtggaat ctttttctcc tcttgtgtcg acgatcatgc
     2101 caggtttttg gcgaccgcta ctgccagcta caagtgacaa gaacaagcgt ctaactatat
     2161 gtggacggcg accgtagcgc agaggaaatt gattcacgta gagacaaaat gacggcagca
     2221 gattccaact cgactcttag caacgtgcaa gtcgcggacg ccgctgcgca gctggctacc
     2281 gtggtgctcg aaggccgcca tctgaagaaa agttacggtc agggtccaaa aattttgccg
     2341 gttttggtgg acgccaacat cactttgcat aagggcgaga tgattgccat tgtcgccccg
     2401 tcgggcgcgg gcaaaagtac tcttctgcat ttgctggccg cgctagacat gccgacaagc
     2461 ggtacagtat acttcgccac gaaagctatt gaatcgaatc aagactcgtt gctcgcggaa
     2521 tttcgcaatc gggccgttgg gtttctctgg caacggcacc agttgttgct tgattttacg
     2581 gccgaagaga atgtggccat gccgctgttg ttgcgcggcg aaaatttcgc ttctgcgcag
     2641 gggatggcgc ggaagtggct tacggaggtt ggcttggaat cccgagctgg ccaccgtgca
     2701 ggggaactct ccggcgggga acagcaacgc gtgtcgattg cccgggcgct tgtcactggg
     2761 ccggccgtgt tgctggcaga tgaaccgacg gggaatttag acgagcagaa tgcttgggct
     2821 gtgttcgagt tgttagttcg gttgcaccgc acgctgggtt tgacgtcgct tatcgcgacc
     2881 cacaatctgg cactcgccgc gcgatgcgat cgggttctga cgctggagca tggcgttctt
     2941 accgctcgca aggctacggc cgctgcgagt gcgacccccg ggggagaagc gagctaacgt
     3001 gttcgaacgt tataccgaaa aagcgcgtcg cgtaattttc tttgccagat atgaggccag
     3061 tcagtacggc agtccttaca tcgagaccga gcacctcttg ctcggcttga tgcgcgagga
     3121 taaagccctg gccaaccgct tcttgcggca acagggatct attgagtcca tccgcaaaga
     3181 gattgaagcg cgcatcacca ttcgcgagcg catctccacc tccgtggagg ttcccctcag
     3241 cgccgaatgc aaacgcatcc tcaatatggc tggtgaggaa gccgagcgtt tgggcgtaaa
     3301 gcacgtcggc accgaacatt tgctgctcgg aatcctgcga gaagaaaagt gttttggcgc
     3361 agaaattttg atggagcgcg gcctgcgact ctccaccttg cgtgaagagt tggctcgtac
     3421 ttccggtgag aaaactacag ccgcgcgccc caaggaaact tctttattgg cggagttcag
     3481 ccgtgatctc acgcaggcgg cttccgaagg ttctcttgat ccgctagtcg gccgcgatgg
     3541 tgaagtagag cgcgtcatcc agattttgtg ccgccgcaca aaaaataatc ccgtgctcat
     3601 cggtgagcct ggcgttggca aaaccgccat cgtcgaaggt ctcgcgcagc gcatctccga
     3661 tggcgatgtt ccaccctttc ttgcggacaa gcgcatcctc tctctcgatc tctccttgat
     3721 cgttgccggc accaagtatc gtggacaatt tgaagagcgc cttaagacca tcatgaaaga
     3781 attgatggag agccagaatt ccatcgtctt tatcgacgaa ctccatactc tagtcggtgc
     3841 tggttccgcg gaaggctcgc tcgacgccgc taacattctt aagcccgcgc tttctcgtgg
     3901 cgaaattcaa tgcatcggag ccactactcc cggcgagtac cgtaagtccg tcgaaaagga
     3961 tcgctctctc gagcgccgct tccagtccgt caaggttccc gcgcccaatg aagaggaagc
     4021 tgtgcaggtc atcagcggcg tgcgcgaacg ttacgaaaag ttccacgccg tcagctatac
     4081 cgatgaggcg ttgaaatatt ccgtctatct ttcaaaccgc tatattccag atcgtttcct
     4141 tccagacaag gccatcgatt tgatcgacga agctggagcg cgcgtgaagt tgcgccaggc
     4201 ctccgttcct gaagaagtcg gcgaagtgca gaagcgcgtc aaattcatca ctcatcgaat
     4261 ggaaaccgcc atcgccaatc atgaatttga gaaggcgcgt ttttactccg aagaagagcg
     4321 caaggaaaaa gaaaatctgc gcggccttcg tgagcgctac aaactcgacg acgcctctac
     4381 cggcatcgtc acccgcgaag acatcgaaga agtcgtctct cgctggactg gcatcggcgt
     4441 ctcctccatc aaggaagacg aacagcagaa ac
//
LOCUS       DATCBB010000487         1649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_16325, whole genome shotgun sequence.
ACCESSION   DATCBB010000487 DATCBB010000000
VERSION     DATCBB010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16325"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1649
                     /locus_tag="VII37_05395"
     CDS             <1..>1649
                     /locus_tag="VII37_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY5069195.1"
                     /translation="SDSASFDNAFELKLLEGLSAEEAMLGLVPPAFEKDPVLSRDVRA
                     ALTAVSQQNEPWDGPAALVFSDGQLVGVKLDRNGLRPLRYTLTHDGLLIAGSETGLID
                     FEESRIAERQRLGPGEMILADPASGLFLRWRDILKRLAANVDRAPATPRAATNFVSHA
                     APMPIEQPKRMAAAAGWTEDQFKTLFSALVHGKEADWSMGDDAPPAFLSTLTRTLWDY
                     CKQRFAQVTNPPIDPLRESHVMSLEVHLKGGVTLPTPLIDAAQLAKLTEVFGEPRRID
                     LTFPAAMGVPGARRAVAQLSTTPLSSGGRPGFLLLSDRGIGKERACLPALLATAALWK
                     GMVREGLWDVPLVVESAQIFDTHHVALLVAAGASAVLPYLADQFADGMESGGAEHVRN
                     SVNAGLRKVLARMGVSTIASYRNSQLFEIVGLSEDLCAEFFEDAADFPGQKSLDELLG
                     DYLRMHEKGFSAAADDLGDAGLYRFRKGAELHANSPELVRRMHAHVRAPDAKTYSALE
                     ELAERQGTVFLRDLLDIVPGTPVAMEEVEPAEEVLKRFSTQ"
BASE COUNT          318 a          404 c          549 g          378 t
ORIGIN      
        1 tcagcgattc cgcgagtttt gataatgcat tcgagttgaa attactagag gggctgtcag
       61 ctgaagaagc gatgctgggg ctcgttccgc cggcatttga gaaggatccg gttttgtcac
      121 gcgatgtgcg cgccgcgctt acggcggttt cgcaacaaaa tgagccttgg gacggaccag
      181 cggcgctagt tttctccgac gggcaattgg tgggcgtgaa gctcgatcgt aatggattgc
      241 gaccgctgcg ctatacgctg acgcatgacg gcttgctcat cgcgggctca gaaactggat
      301 tgattgattt tgaagaatcg cgcatcgcgg aacggcagcg tttgggtccg ggggaaatga
      361 ttctcgctga tccggcgtcg gggcttttcc tgcgctggcg cgatattttg aagcggttgg
      421 ctgccaacgt tgatcgcgcg ccggccacgc cgcgagctgc gaccaatttt gttagccatg
      481 ccgcgcctat gcccattgaa caaccaaaac gcatggcggc tgcggcgggc tggacagaag
      541 atcaatttaa aacattgttt tccgcgctgg tgcatggcaa agaagctgac tggagtatgg
      601 gcgatgatgc gcctccggca tttctttcca cgctgacacg gacgctgtgg gattactgca
      661 agcaacgttt cgcgcaagtg accaatccgc ccatcgatcc gctgcgcgaa tcgcacgtga
      721 tgtcgctcga agtgcattta aagggcggcg ttactttgcc gacaccatta attgatgctg
      781 cgcagttggc aaaacttacc gaagtgtttg gtgagccgcg ccgcatcgat ttgaccttcc
      841 ctgccgccat gggagtcccg ggcgcgcgcc gcgctgttgc tcagctttca actacccctc
      901 tgagtagcgg cggacgcccg ggattcctcc tccttagcga tcgcggaatc gggaaggaac
      961 gcgcgtgctt gccggcgctg ttggcgacgg cggcgttgtg gaaagggatg gtgcgcgaag
     1021 gattgtggga tgtgccgctg gtggtggaat cggcgcagat ttttgatacg catcatgtgg
     1081 cgctgttggt ggctgcgggt gcgagcgcgg tgttgccata cctggctgat caatttgcgg
     1141 atgggatgga aagcggcgga gcggagcacg tgaggaactc cgtgaacgcc gggttacgaa
     1201 aagttttggc gcggatgggc gtgtctacga ttgcaagtta tcggaacagc cagcttttcg
     1261 aaattgtggg gctgtccgaa gatttgtgcg cggaattttt tgaagatgcc gccgattttc
     1321 ctgggcagaa gagtttagat gagctgctgg gcgattattt gcgcatgcat gaaaaaggtt
     1381 tttctgctgc ggcggatgat ctgggcgatg cggggctgta ccgcttccgt aaaggcgctg
     1441 agctgcatgc taattctccg gaactggtgc ggcgcatgca tgcgcacgtg cgcgcgccgg
     1501 atgcaaagac atattccgcg ctggaagaac ttgcggagcg gcagggaacg gtatttctgc
     1561 gcgacttgct ggatattgtg ccgggaacgc cggtcgccat ggaggaagtg gaacccgcgg
     1621 aagaggtgct gaagcgtttc agcacgcag
//
LOCUS       DATCBB010000488         1393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_146284, whole genome shotgun sequence.
ACCESSION   DATCBB010000488 DATCBB010000000
VERSION     DATCBB010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146284"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VII37_05400"
     CDS             <1..496
                     /locus_tag="VII37_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069196.1"
                     /translation="LLELAMAADRVYMLDTQEGEARATVALSEMNFGPLPMVNHLSRL
                     DARFYQDETRVKDLRARAGQELAPSEAVEAGLVTAAPDDLDWQDELRTAIESRVALSP
                     DALTGLEANLRFGLPETMETRVFGRLSAWQNWIFIRPNAVGPSGALKVYGSGSPVKFN
                     WERV"
     gene            526..>1393
                     /gene="boxB"
                     /locus_tag="VII37_05405"
     CDS             526..>1393
                     /gene="boxB"
                     /locus_tag="VII37_05405"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEY5069197.1"
                     /translation="MIDYTEKIPNNVDLGRDRVLQRALEHWQPAYMSWWYDMGPTESH
                     NYEVYLRTAITVEPDGWAKFGYVRMPEYRWGIFLQPRSAERAVNFGTHKGDPAWQEVP
                     GEYRSTLRRVIVTQGDTEPASVEQQRMLGMCCPSLYDLRNIFQVNVEEGRHLWAMVYL
                     LHRYFGRDGREEAEALLERRSGDVDNPRILGAFNEPTPDWLAFYMFTFFTDRDGKFQL
                     SALAESGFDPLARTCRFMLTEEAHHMFVGETGVSRILQRTCEVMREHKVETPGEVRAL
                     GVIDLQTMQKYLN"
BASE COUNT          306 a          324 c          455 g          308 t
ORIGIN      
        1 gttgttggag ttagcgatgg cggccgatcg tgtctacatg ctggacacgc aagagggaga
       61 ggcgcgcgcc acggttgcgt tgtcggaaat gaattttggg ccactgccga tggtcaatca
      121 tctttcgcgt ttagacgcgc gtttttatca ggatgaaaca cgcgtcaaag acttgcgcgc
      181 gcgagctggt caggaactgg cgccgtccga ggcagtggag gctggattgg tcaccgccgc
      241 gccggatgat ctggactggc aggatgagtt gcgcaccgcg atcgagtcgc gagtcgcgct
      301 ttcgccggat gcactgacag gactggaagc aaatttgcga tttggattgc ccgaaaccat
      361 ggagacgcgc gtttttggaa gattatcagc gtggcagaac tggattttca tcaggcccaa
      421 tgcggtcggg ccgagcggag cattgaaagt ctacggcagc ggttcaccgg tgaaattcaa
      481 ctgggaaaga gtttaggcca gcttggtgat gcggggagag cagcaatgat cgactacacg
      541 gaaaaaattc cgaacaacgt ggacctgggg cgcgatcggg ttttgcagcg ggcgctggaa
      601 cactggcaac cggcctatat gagctggtgg tacgacatgg gcccaacgga gagccacaat
      661 tacgaagtgt atttgcgcac ggcaatcacc gtggagcctg atggctgggc taaatttggt
      721 tatgtgcgca tgccggaata tcgatgggga atttttttgc aaccccggag tgccgagcgc
      781 gcagtgaatt tcggaactca caaaggcgat cccgcgtggc aagaagttcc cggggaatac
      841 cgatcgacgc tgcgccgcgt aatcgttacc cagggagata cggaacccgc ttccgtggaa
      901 cagcagcgca tgctgggtat gtgctgcccc tcattgtatg acctgcgaaa tatttttcag
      961 gtgaacgtgg aagaaggacg gcatttgtgg gccatggtgt atttgttgca ccgctatttt
     1021 ggccgcgatg gacgggaaga ggcggaggcg ttgctggagc gacggtcggg agacgtcgat
     1081 aatccgcgga tccttggggc ttttaatgag cccacccccg actggctggc gttttacatg
     1141 tttacgtttt ttacggatcg cgacggtaag tttcaattaa gcgcgcttgc ggaatcggga
     1201 ttcgatcccc tggcgcgtac gtgccggttc atgctgacgg aagaagcaca ccacatgttt
     1261 gtgggcgaga ccggagtgag tcgtattctg caacgtacct gcgaagtgat gcgtgagcac
     1321 aaagtggaga ctcctggcga ggtgcgtgcc cttggggtga tcgatctaca aacgatgcag
     1381 aaatatttga att
//
LOCUS       DATCBB010000489         1907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_242367, whole genome shotgun sequence.
ACCESSION   DATCBB010000489 DATCBB010000000
VERSION     DATCBB010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242367"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VII37_05410"
     CDS             <1..804
                     /locus_tag="VII37_05410"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5069198.1"
                     /translation="SPPQPWESSNFLELPAGSNSIHKLSAPARLDEPPQTARLTVTSY
                     NIHSCEGLDLRVRCDRIAAILIHTHADVIALQEARAGQAEQIARLLGFHVLFARADRV
                     GGYEFGNAILSRFPIRRSDVYPLGVPHHEQRVCLHAEIAWPDDVSALHVFSAHLGQNE
                     TERRQQAERLASNSILENPSFHNAPRVLLGDFNEKSRNGIVNQSLASYQCATGRSWTA
                     FCPILYLDRIYISSDLKFQDFHIDRNGAALIASDHAPITAVLLNAANSH"
     gene            complement(849..1505)
                     /locus_tag="VII37_05415"
     CDS             complement(849..1505)
                     /locus_tag="VII37_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEY5069199.1"
                     /translation="MNIELNENEARVLGALLEKEITTPDYYPLSLNSLVNACNQKSNR
                     EPVMNLDDDAVRGALRTLHDNSLARSVSAADSRVTKYEHRLQEAFNFDRREAALFCEL
                     LLRGPQTPGELRTRAERMHRFDDLSEAQSALQRLMNREPPLVKVLARQPGTKESRYTH
                     LLSGDAQPVSTGAGREVPATLERRKMDEFARLSSEIAELRKDMADLKQQFAAFRKQFE
                     "
     gene            complement(1663..>1907)
                     /locus_tag="VII37_05420"
     CDS             complement(1663..>1907)
                     /locus_tag="VII37_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069200.1"
                     /translation="PMPGLVEWQFTESAEVQLYEQKEHAGHSILTVGVLPLEAERKRL
                     EEAGLEPGPIEEAKDFFIMRIRDPDQNLVVLASAKR"
BASE COUNT          414 a          588 c          473 g          432 t
ORIGIN      
        1 tcgcctccgc agccttggga aagttccaat tttttagaat tacccgcggg atccaacagc
       61 atccataaat tgtccgcccc cgcaagactt gatgaacccc cgcaaaccgc acgactgacc
      121 gtcacttcat acaacatcca ctcctgcgag ggtctggacc ttcgcgtccg gtgtgatcgc
      181 attgccgcaa ttctcattca cactcacgcc gatgtcattg ctctccagga ggcacgcgca
      241 ggccaggcag agcagatcgc acgtcttctc ggattccatg tcttattcgc gagggctgat
      301 cgcgtgggcg gttacgaatt tggcaacgcc atcttaagcc gctttcccat tcgaaggtct
      361 gacgtttatc cgctcggcgt gccccaccac gagcaacgcg tgtgcctgca cgcggaaatt
      421 gcttggccgg acgacgttag cgcgctccac gtgttcagcg ctcaccttgg tcagaatgaa
      481 acggaaaggc ggcaacaagc tgaacgcctc gcgtccaact ccattctaga aaacccttcg
      541 ttccacaatg cgccgcgggt actcttggga gacttcaatg agaaatcgcg aaatggtatc
      601 gtgaaccagt cccttgccag ctaccaatgc gccaccggaa gatcgtggac cgcattttgc
      661 cccatcctgt atctcgatcg catctacatc agcagcgacc tcaaatttca ggactttcac
      721 attgatagaa atggcgctgc cttgattgcc tcggaccatg ccccaatcac ggccgtccta
      781 ctaaacgctg cgaactccca ctgactgcgg acaatctcgg cgggcggcac aaacttacaa
      841 gcaacgccct attcaaactg ctttcggaaa gccgcgaatt gctgctttag atctgccatg
      901 tcttttcgaa gttcggcaat ttctgaggac aagcgagcaa actcatccat ttttctccgt
      961 tccagcgtcg cgggaacttc ccgtccagcc cctgtactaa ctggctgcgc atctcctgac
     1021 aataaatgcg tgtagcggga ttccttcgtg cccggctgcc tcgcgagtac tttgacaagc
     1081 ggtggctcgc ggttcatcaa tcgctgcagc gccgactgcg cttcgctcaa atcgtcaaat
     1141 cgatgcatcc gctcggcgcg cgtccgcagt tcgcccggtg tttgtggccc gcgcagcagc
     1201 aactcgcaga ataaggccgc ttcgcgccgg tcgaagttaa acgcctcctg tagacggtgt
     1261 tcgtacttgg tgacgcggct atccgcggcg ctgaccgagc gagccagaga attgtcgtgt
     1321 agcgtacgca atgcgccgcg aaccgcgtcg tcgtccaggt tcatgaccgg ctcgcgattg
     1381 gatttctggt tgcacgcgtt gaccagcgag ttgagcgaaa gcggataata atccggcgtg
     1441 gtgatttctt tttccagcag cgccccaagc actcgcgctt cattttcatt cagttcgata
     1501 ttcacagcac caaatcatac cggaaccgcg ctgcacgccc aacatggaac tgaggcgatc
     1561 cttcaactcc tgatcaaaat attaatcgta gtgggaaggc gcttgtcgag taacgcaaag
     1621 cacatgaata ctctctcgag cacgcaagag tccgcttatc aattaccgtt tcgcgctagc
     1681 gagcacgact aagttctggt caggatcgcg aatgcgcatg atgaagaagt ccttagcctc
     1741 ttcaatcggg ccgggttcca atcccgcctc ctccaagcgt ttccgctccg cttccaatgg
     1801 aaggacgccg acagtcagaa tgctatgccc ggcgtgttcc ttttgctcgt aaagctggac
     1861 ctcggcgctt tccgtaaact gccattccac taaaccaggc atgggac
//
LOCUS       DATCBB010000490         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_146342, whole genome shotgun sequence.
ACCESSION   DATCBB010000490 DATCBB010000000
VERSION     DATCBB010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146342"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2327
                     /locus_tag="VII37_05425"
     CDS             <1..>2327
                     /locus_tag="VII37_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5069201.1"
                     /translation="YGVSLETVRKALQSANANLAKGAVSNDKKIVNIADTDQIFKAYE
                     YQPLIIAGHAGSALRLQDVAQVVDSLEDTRNLGLAGGQRSVILAVFRQPGANMIATVD
                     RILPMLPMLRASISPSLHLNVLIDRTLTIRSSVHDVEVALIISILLVIFVVFAFLRNV
                     WATIIPSVAVPLSLVGTFGVMYLCHYSLDNLSLMALTICTGFVVDDAIVVIENIARHI
                     ESGMAPYAAAMKGAREIGFTVISMSASLVAVFIPILMMGGIVGRLFREFAVVLSVAIV
                     VSLFISLTTTPMMCARFLRHDTGRHNRLYRFSERAFNRVLSMYDFSLKWVLRHQFLIL
                     VLTIGTVCLNIYLFAKTPKGFFPQQDTGRIAGLIQGDQDLSFPAMAEKMRAFTDIVLQ
                     DPAVDTVASFAGGGNGGGTARMFASLKPLKERKISADEVIGRIREKTAAIPGASLFLQ
                     AVQDLGIGGRFGGAQYQYTLQADNLDDLNHWAPILMERMRAIPELRDVNSDQQMKGLQ
                     TSLFIDRDTASRMGVNVLDIDNTLADAFGQRQVSNIYKGMNQYHVVMEVAPEAQEGPQ
                     ALNTIYVPSKSGKLIPLSAFARYEPSNTALSVNHQGVFPAITLSFNLAPGASLGPAVE
                     HIEAAQRELRIPTTVHTMFQGNAQAFQDSLATEGILILTALAAVYIVLGILYESYIHP
                     ITILSTIPSAGIGALLAIRFMKSDLNIIALIGIILLIGIVKKNAILMIDFAVQVEREG
                     HKSPVEAIYEACLLRFRPILMTTMAALLGALPLAL"
BASE COUNT          477 a          676 c          616 g          558 t
ORIGIN      
        1 gctacggcgt aagcctggaa acggtgcgca aagcattgca gtccgccaac gccaatctcg
       61 ccaaaggcgc ggtctctaac gacaaaaaga tcgttaacat cgccgacacc gatcaaattt
      121 tcaaagccta cgaataccag ccgctgatta ttgccggaca cgccggctct gccctccggc
      181 tgcaagacgt ggcgcaggtt gtcgattcgc tcgaggacac ccgcaatctg ggtctcgccg
      241 gcggacagcg gtcggtaatc ctcgcggttt tccggcagcc cggcgcaaac atgattgcca
      301 ccgtcgatcg catcctgccc atgcttccga tgctgcgcgc ttccatctcg ccttcgctgc
      361 atttgaacgt gctcattgat cgcacgctga ccattcggtc gtcggttcac gatgtggaag
      421 tcgccctgat catctccatc ctgctggtga tttttgtggt cttcgcgttt ctgcgcaatg
      481 tgtgggccac gatcattccc agcgtggccg ttccgctttc cttagtcggc actttcggcg
      541 tgatgtacct ttgccactac agcctcgaca atttgtcctt aatggcgctg accatttgca
      601 ccgggttcgt cgtcgatgac gccatcgtgg taattgaaaa tatcgcgcgg cacattgaat
      661 caggaatggc tccctacgcg gcggcgatga aaggcgctcg ggagattggt ttcacggtta
      721 tttccatgag cgcctcgctc gtcgcggtat ttattcccat attgatgatg ggcggcatcg
      781 tgggccggct cttccgtgaa tttgccgtcg tcctcagcgt ggccatcgtg gtgtcgctgt
      841 tcatttcgct tactaccaca ccgatgatgt gcgcgcggtt tttgcggcac gacaccgggc
      901 ggcacaaccg gctctaccgt ttttccgagc gggcctttaa tcgcgtattg agcatgtacg
      961 atttttcgct gaagtgggtg ctgcgccacc agtttttgat cctggtgctt actatcggca
     1021 cggtgtgcct caacatttat ttgttcgcca agacgccaaa aggatttttc ccccaacagg
     1081 ataccggacg catcgctgga ctgattcagg gcgatcagga cctatctttc ccggccatgg
     1141 ctgaaaagat gcgcgcgttt accgacattg ttttgcaaga tccagccgtc gacacggtgg
     1201 cctcctttgc tggcggcggc aacggaggcg gcacggcgcg catgttcgcc tcgctgaaac
     1261 ctttgaaaga acgcaaaatc agcgctgacg aagtgatcgg gcgcattcgc gaaaagaccg
     1321 cggcaatacc gggagcttct ttatttcttc aggcggttca ggacctgggc atcggcggcc
     1381 gttttggtgg cgcgcaatac cagtacacct tgcaggctga caatcttgac gacctgaatc
     1441 attgggcgcc gatcctcatg gagcgcatgc gcgccatccc cgaactgcgc gacgtaaata
     1501 gcgatcagca aatgaaaggg ctgcaaacat cgctgtttat cgatcgcgac accgcctcac
     1561 gcatgggcgt gaatgttctg gatatcgaca acactttggc cgacgccttc ggacagcgcc
     1621 aggtttccaa tatttataaa gggatgaatc aatatcacgt cgtgatggaa gttgcgccag
     1681 aagcgcagga agggccgcaa gcgctcaaca cgatttatgt cccatcaaaa agcgggaaac
     1741 taatcccact gagcgccttc gcgcgttacg agccgtccaa taccgcgcta tccgtcaatc
     1801 accagggcgt atttccggcc atcacgctgt cattcaatct tgcgcccgga gcttcactag
     1861 gtccggccgt cgagcacatt gaagcggcgc aacgtgaact gcgaattccc actaccgtgc
     1921 acactatgtt tcaaggtaac gcgcaagcgt ttcaggattc tctcgccacg gaaggcatct
     1981 tgattctcac tgcgttggcc gctgtgtata tcgtgctcgg cattctttac gaaagctaca
     2041 ttcaccccat caccattctg tctaccattc cttcggcggg cataggcgcg ctgcttgcca
     2101 tccgtttcat gaaatcggat ctgaacatca ttgcgctgat tggaattatt ctgctgatcg
     2161 gcatcgtgaa gaaaaacgcc atcttgatga tcgacttcgc ggtgcaagtg gaacgggaag
     2221 ggcacaaatc cccggtggaa gccatctacg aagcgtgcct gctgcgcttc cgccccattt
     2281 tgatgaccac catggcggcg ttacttggcg ctttaccact ggcatta
//
LOCUS       DATCBB010000491         1259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_372710, whole genome shotgun sequence.
ACCESSION   DATCBB010000491 DATCBB010000000
VERSION     DATCBB010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372710"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..552)
                     /locus_tag="VII37_05430"
     CDS             complement(154..552)
                     /locus_tag="VII37_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069202.1"
                     /translation="MNRDIKIKAIAFGVLAGTLMSSWPARANTIFLQCPGAPSDYADT
                     LTVDLTNNTVNNHSAKKINKTAIDWQHDVKCGDCTSSNPGTATQIYHLDRTTGNLAIH
                     DEFHMQDGTGGRNGPFTYHCAVGHAPATKF"
     gene            777..>1259
                     /locus_tag="VII37_05435"
     CDS             777..>1259
                     /locus_tag="VII37_05435"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY5069203.1"
                     /translation="MTVVSAQKLRIEIGNAASVSALLLRPQRARACFVFAHGAGAGMT
                     HPFMETVATGLSDRGVATLRYQFSYMEKSSKRPDSPAVAHATVRAAVTEAARCCPGLP
                     LIAGGKSFGGRMTSQAQAIAPLAGVVGLAFMGFPLHPANKPSSERAKHLSDIDVPMFF
                     V"
BASE COUNT          234 a          343 c          384 g          298 t
ORIGIN      
        1 taagttgctc aggacgttca gcggcatgcc cggtttccgg ccagtctcga ttgcgtcgcc
       61 gatgcctttc cagcgccgtt tgcccggcgt ttagagcagc ggacgtgctg cgatcttgtt
      121 agagcgtgac agtggacagc aggtcagctt ggtctaaaat ttcgtcgctg gcgcgtggcc
      181 cacagcgcag tggtaagtga atgggccgtt ccgtccaccc gttccatcct gcatgtgaaa
      241 ttcatcgtgt attgccaggt ttccggtggt tctgtcgaga tgatatatct gcgttgccgt
      301 ccctgggtta gaggaggtgc agtcgccaca ttttacgtcg tgttgccaat ctattgctgt
      361 cttgtttatt ttttttgccg agtgattgtt cacggtgtta tttgttaaat ctaccgtcag
      421 ggtgtcggca tagtcgctgg gcgcgcctgg gcattgaaga aatatcgtat ttgctcgcgc
      481 cggccatgaa gacatcaacg tcccggccag cacgccaaag gcgatggcct tgatcttaat
      541 gtctcgattc atggtacttc tcctgtggag cggaatcagg atttacagat gacaacaacg
      601 caggcgtggt tcgtactatc cgggcttagg cacgatacga ccatcacaat cgtaactctt
      661 ttcgttggta gcgtagtgga tcggatgcgg acagagctga ttgcggccat ttccacattt
      721 gagccaggga aaaagccagc cgggtgcgcc aaaaaaccaa ataccggccg gatcagatga
      781 cagtcgtcag cgcgcagaag ctgaggatcg agatcgggaa tgcggcttcc gtctccgcgc
      841 tgctgcttcg gccacagcga gcgcgcgcgt gttttgtctt cgcgcatggc gcgggcgccg
      901 ggatgaccca tccgttcatg gagacggtgg caaccgggct atccgaccgc ggcgtcgcaa
      961 cgttgcggta tcagttttcg tacatggaaa aaagcagcaa gcggccggac agcccggcgg
     1021 tcgcgcacgc cacggtgcgg gccgcggtga cggaagccgc gcggtgttgt ccgggactcc
     1081 ccctgatagc cggcggcaaa tcgttcggcg gacgcatgac gtcgcaggcg caggcgattg
     1141 cgccgctggc tggcgtcgtt gggcttgcct tcatgggctt tccattgcat ccggccaaca
     1201 aaccgtccag cgaacgggcc aagcatcttt ccgatatcga tgtccccatg ttctttgtg
//
LOCUS       DATCBB010000492         1009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_340401, whole genome shotgun sequence.
ACCESSION   DATCBB010000492 DATCBB010000000
VERSION     DATCBB010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340401"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..397
                     /locus_tag="VII37_05440"
     CDS             143..397
                     /locus_tag="VII37_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069204.1"
                     /translation="MCRLRCHAPESEERQAKKHAANRAIISHVDLHQRPVPDNGTAYQ
                     KIGGAFAPESPGKGLGNHNSARASRPSRTRIAIVLTYINA"
     gene            445..897
                     /locus_tag="VII37_05445"
     CDS             445..897
                     /locus_tag="VII37_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5069205.1"
                     /translation="MHSIFVRVFLVAAILVGAIPVAHAQKSDLAAQNFDRGKYEYEAH
                     CAVCHGMSGIGDGSFAQLFRAGTLMPNLTEFSKKNNGVFPFKRVYEAIDGTQNVEAHG
                     TQLMPVWGPRYKFEAGRRFDDDFPADSEAFVRARILALTEYVYRLQAK"
BASE COUNT          243 a          253 c          288 g          225 t
ORIGIN      
        1 acggcagcgg gattcattga tcctcatcaa acatcgggtt gtcacgcgtg gatattgacg
       61 tggggggagg tacgctgtgc gatgggcttt ctgtcgtggg tttgcgcgat caccaacctg
      121 aacaaccatt tagcgcacac tattgtgccg tttgcgctgt cacgcgccgg aatcggaaga
      181 gcgccaggcc aagaaacacg ccgccaatcg agcgatcatc agccacgttg atctgcatca
      241 acgcccggtg cccgataacg gaacagccta ccaaaaaatt ggcggcgcat ttgcgccaga
      301 aagtcctgga aaaggtttgg gaaatcacaa cagcgcgcgg gcatcacggc ccagccgaac
      361 acgcatcgcc atcgtgttga cctacatcaa tgcgtagtga tttgaagagt ttctccgcgc
      421 cgcttcgtct gacgggagca aaatatgcat tcaatctttg ttagagtatt tttagtcgct
      481 gcaatcttag tcggcgcaat accggtggcg cacgcgcaaa agagcgatct tgcagcgcaa
      541 aatttcgata ggggaaagta tgaatacgag gcccattgcg ccgtctgcca tggcatgagc
      601 ggtataggtg atggatcgtt tgcgcagctc ttcagagcag gaacccttat gccaaacctt
      661 actgagtttt caaaaaagaa taacggggtg tttccgttca agcgcgtata tgaagccatt
      721 gatggcacac agaacgtgga ggcgcatggg acgcagctga tgcccgtttg gggccctcga
      781 tataagtttg aggcaggaag gagattcgac gacgactttc ccgcagattc cgaggccttt
      841 gtgcgtgctc gtatcctcgc attgaccgaa tacgtctacc gcctgcaagc gaagtaatat
      901 ttatatttcc agtgcaacgt atcgccggtg ggctcgccag cgtcgggctc gccagcgtcg
      961 ggcttgggga aaaagcggat gaagcagctg tgagcaaatt tgcgttgat
//
LOCUS       DATCBB010000493         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_146529, whole genome shotgun sequence.
ACCESSION   DATCBB010000493 DATCBB010000000
VERSION     DATCBB010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146529"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VII37_05450"
     CDS             complement(<1..435)
                     /locus_tag="VII37_05450"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY5069206.1"
                     /translation="MVLTEEQKRERIARRIALELRDGFYVNLGIGMPTAVANYVPQGM
                     EVVLQSENGMLGVGPYPLESQVDPDLINAGKETVTELPGTCYFSSSDSFGMIRGGHVD
                     LTVLGALQVDEKGTLANWAIPGKMLKGMGGAMDLVAGAKRVFI"
     gene            complement(472..1167)
                     /locus_tag="VII37_05455"
     CDS             complement(472..1167)
                     /locus_tag="VII37_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY5069207.1"
                     /translation="MDKRTPSADAAIEKLTDGVTILMGGFGLCGIPENLIAAVRRKGT
                     KNLTIVSNNAGIDDFGIGILLQSRQVKKMISTYVGENKLFERQVLSGELEVELNPQGT
                     LAERLRAGGAGVPAFFTPTGVGTMVAEGKETREFDGRQYVMERALRGDFAFVKAWKGD
                     RWGNLIYRKAARNFNPIMATAADYVIAEVEELVDLGQLDPDSIHTPGIFVNAIFQGMA
                     YEKRIEKHCIRKD"
     gene            complement(1248..2222)
                     /locus_tag="VII37_05460"
     CDS             complement(1248..2222)
                     /locus_tag="VII37_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY5069208.1"
                     /translation="MSPAHLINRVSILGTGLIGGSFALALRKYTEGIHITAWDRADTV
                     SRAKDIGAADEIFSGELKPAIQSADLIYIGLPISTTLDVLPEIARHSPQHALVTDACS
                     TKVRIVHAADELFPRSDEKLFLGGHPMAGKESSGIANADANLFRDTTYALIGESSSNV
                     DDAVTNSEVAQQSEAGGERAPSQRARPTPSFKEDPRVIAFLKIISKIGARPLWLGAQQ
                     HDYAVGLASHLPQLAAVALASFLYDHLDENGLPIVLAGPGLRDSLRLAGSPYSTWRDI
                     VLTNREVLSAGLDLFARRLDELRDKLASRELEADFDAANELYKLLRSF"
BASE COUNT          472 a          607 c          612 g          538 t
ORIGIN      
        1 gatgaacacg cgcttcgcgc cggccaccag atccatcgcg ccgcccatgc ctttcaacat
       61 ttttccaggt atggcccagt tggcgagcgt acctttttcg tccacttgca gcgctcccaa
      121 aacggtgaga tcgacgtgtc cgccgcgaat catcccaaat gaatctgagg atgaaaaata
      181 acaagtgccc ggtagctcag tgacggtttc tttgcctgca tttattaaat ctggatcgac
      241 ttgagattcc aatgggtagg gtccgacacc cagcatgccg ttttcggatt gcaaaaccac
      301 ctccatgccc tgcggcacat agttcgccac ggcggtcggc atgccgatgc ccaaatttac
      361 ataaaaaccg tcgcgcagtt ctagtgcgat gcgccgcgcg atgcgttcac gtttttgttc
      421 ttcagttaga accatgtacg cctttcagtt ctgcgtcccc aacgtggggc actaatcttt
      481 tctaatacag tgtttttcaa tgcgcttttc gtatgccatt ccctgaaaga ttgcgtttac
      541 aaaaattccc ggcgtgtgaa tagagtccgg atccagttga ccgagatcga ccaattcctc
      601 gacttcggcg atgacgtaat ccgctgctgt ggccatgatg ggattaaaat tccgggcggc
      661 tttgcggtaa atgagatttc cccagcggtc gcctttccag gcttttacaa atgcgaagtc
      721 gccgcgcagg gcgcgctcca tgacgtactg tcgtccatca aattcgcgtg tttctttgcc
      781 ctcggcgacc atggtgccca ctccggtggg tgtaaagaac gccggtacgc cggcgccgcc
      841 ggcgcgcaaa cgttctgcca aggtgccttg gggatttagc tccacttcca gttccccgct
      901 gagcacttgg cgttcgaaca atttattttc tcccacataa gtggagatca tttttttgac
      961 ctggcggctt tgtagaagta tgccgatgcc gaagtcgtcg atgcccgcgt tgttgctgac
     1021 gatggtgaga ttttttgtgc ctttacggcg aaccgcggcg attaaatttt ctgggatacc
     1081 gcacaaacca aatccgccca tcaatatggt tacgccatcg gttaactttt cgatggccgc
     1141 atcagccgaa ggagttcgtt tgtccatgaa aactctcgcg aataggagct tagtgtaatg
     1201 caacctgtcg cgccggcgac aggatgaatg cccgcgtttt gtcgtgctca gaaactacgt
     1261 agcagtttgt acaattcgtt ggcggcgtcg aagtcggctt ccagttcgcg ggaagcgagc
     1321 ttgtcgcgca attcatctag gcggcgagcg aacaaatcga gtccggcgga aaggacttcc
     1381 ctgttggtca gcacaatgtc gcgccaggtg ctgtaaggac tgcctgcgag cctcagcgag
     1441 tcgcgcaggc cgggcccggc cagtacgatg ggcaagccgt tttcatccag gtgatcgtaa
     1501 agaaacgacg ccagcgcgac tgctgcgagt tgcggaagat gcgatgcgag acccacggcg
     1561 taatcgtgct gttgtgcgcc gagccacagg ggacgcgcgc caattttgct aatgattttc
     1621 agaaatgcaa tcacacgtgg atcttctttg aacgaagggg taggacgcgc gcgttgcgat
     1681 ggcgcccgct cgccgcccgc ttcggactgc tgcgcaactt cggaattcgt gactgcgtca
     1741 tcgacgttgg acgaagattc accaatgagt gcgtaggtgg tgtcgcgaaa caaatttgcg
     1801 tcagcattcg caattccgga cgattctttt ccggccatcg gatggccgcc aagaaaaagt
     1861 ttttcatcac ttcgtggaaa taattcgtcc gcagcgtgca cgatacgtac cttggtgctg
     1921 caggcgtcgg tcaccaaggc gtgttgcggg ctgtgacgcg caatctctgg cagaacgtca
     1981 agggtggtac tgatcggtag cccgatgtaa atcaggtcgg cactttgtat ggcgggcttc
     2041 agttcacccg aaaaaatctc gtccgctgcg ccgatatcct ttgcacgtga cacggtatcc
     2101 gcgcgatccc acgcagtgat gtgaatccct tccgtatatt ttcgcagcgc caaggcaaac
     2161 gagccgccga tcaagccggt gccgaggatg ctgacgcgat taatgaggtg tgctggcgac
     2221 atgaatcag
//
LOCUS       DATCBB010000494         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_372899, whole genome shotgun sequence.
ACCESSION   DATCBB010000494 DATCBB010000000
VERSION     DATCBB010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372899"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VII37_05465"
     CDS             <1..368
                     /locus_tag="VII37_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY5069209.1"
                     /translation="APERARQWYKAVQDAQVAAIAAVKNGVLCGDVDAAARQVLEGQG
                     LGKYFVHSTGHGLGLEVHEEPRIARGQKKRLEAGNVVTIEPGIYIEGVGGIRIEDDVA
                     VHANRIEVLTRITRDLIEI"
     gene            376..927
                     /gene="accB"
                     /locus_tag="VII37_05470"
     CDS             376..927
                     /gene="accB"
                     /locus_tag="VII37_05470"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY5069210.1"
                     /translation="MTRKKISKQITNKATDSFANLELKQIEQLLQFMTEHNLEEFEYS
                     RGDLRIRLRKPSTGVVLQQTRSGYAPEIIVPGGGSGTTASANTQSTAGGADGRGSEDL
                     HLIKSPIVGTFYGAPSPGAEAFVKVGSQVETGQTLCIVEAMKLMNEIESDASGEVIRV
                     YVENGQPVEYGQPLFGIRPGRKK"
     gene            943..>2069
                     /locus_tag="VII37_05475"
     CDS             943..>2069
                     /locus_tag="VII37_05475"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY5069211.1"
                     /translation="MFQKILVANRGEIALRVICACKELGISTVAVYSEADRNSLHVRF
                     ADEAVCIGPARSSESYLNIPQVISAAEITNTDAIHPGYGFLSENANFAKVCEASEIAF
                     IGPTPEIIEMMGDKDRARREVRAAGLVTVPGSDGIIHGEEQLAASAEKIGYPLILKAS
                     AGGGGRGMRIVRRVEDLLAAFQAASSEAQQAFGNPDMYAEKFIEHPRHIEYQVLGDQH
                     GHIIHLGERECSIQRRHQKLVEESPSPAIDAKQRKELGAKVVKALGKIGYTNAGTVEF
                     LLDEDGSIYFIEMNTRIQVEHPVTELVTGVDLIKAQIRIAAGERLQDATGEIHFSGHA
                     IECRINAEDPETFVPSAGRITTFQAPGGTGVRVDSAAHADAV"
BASE COUNT          504 a          455 c          709 g          401 t
ORIGIN      
        1 aggcaccgga aagagcccgg caatggtata aggctgtcca ggatgcgcaa gtagcggcga
       61 ttgccgcggt aaaaaatggc gttctgtgtg gagacgtgga cgcggcggcg cggcaagtgc
      121 tagagggtca gggactaggc aaatattttg tgcacagcac tggtcacggg ctaggtttag
      181 aggttcatga ggaaccgcgc attgcgcgag ggcagaaaaa gcggcttgaa gcggggaacg
      241 tggtgaccat tgaaccggga atctatatcg agggcgtggg cgggatacgc attgaagacg
      301 atgtggcggt gcacgccaat cgcatagaag tgttgacccg cattacgcgg gatctcatcg
      361 agatttgacg accgaatgac gcgcaaaaaa atttctaaac aaataaccaa caaggcgacg
      421 gacagcttcg caaacctgga attgaagcaa atcgagcaac tattgcagtt catgacggag
      481 cataaccttg aggagtttga atactcgcga ggggacctgc gtatccgttt gcgcaagccg
      541 tcaacgggag tggtgttgca gcagacgcgg agcggatatg ctcctgagat aattgtgccg
      601 ggcggcgggt ccgggacaac agctagtgcg aatacgcagt caacggccgg tggcgcggat
      661 gggcgcggca gtgaagattt acatctgatc aagtctccca tcgtaggaac gttttacggg
      721 gcaccgagcc cgggggcgga agcttttgtg aaggtcggat cacaggtgga aacggggcag
      781 acgctgtgca tcgtggaagc catgaagttg atgaacgaga ttgaatccga tgccagtggg
      841 gaagttatac gggtatatgt cgaaaacggg cagccggtgg aatacgggca gccgttgttt
      901 ggcatccggc cgggcaggaa gaaatagcca cacctcagag ccatgttcca aaaaatcctt
      961 gtagctaatc gaggggaaat tgccctgcgg gtcatctgcg cctgcaagga actgggcatt
     1021 tccacggtgg cggtgtatag cgaagcggac cgcaattcgc tgcatgtgcg gtttgcggat
     1081 gaagcggtgt gcatcggacc ggcgcgcagc agcgagagtt acctgaatat tccgcaagtg
     1141 atcagcgcgg cggagattac gaatacggat gcaattcatc cggggtacgg atttctttcg
     1201 gagaatgcca acttcgcgaa ggtttgcgag gcttcggaaa ttgcgttcat cgggcctacg
     1261 ccggaaatca tcgagatgat gggggataag gaccgcgcgc ggcgagaagt tcgcgcagcg
     1321 ggactggtga ccgttcctgg tagcgacgga attattcatg gtgaagaaca actagctgcc
     1381 agcgcggaaa aaattggcta cccgctaata ttaaaggctt cggccggcgg cggcgggcgc
     1441 gggatgcgca tcgtgcggcg ggtggaagat ttgttggcgg cgtttcaggc ggcgagcagc
     1501 gaggcgcagc aggcttttgg caatccggat atgtatgcgg agaaatttat cgagcacccg
     1561 cgacatatcg aataccaggt gctcggcgac cagcacgggc acattattca cctgggcgaa
     1621 cgggagtgca gtattcagcg gcggcaccag aaattagtgg aggagtcacc gtcaccggcg
     1681 attgatgcga agcagcgcaa agagctgggg gctaaagttg ttaaagctct gggaaagatt
     1741 ggatatacga acgcagggac agtggaattt ttgctcgatg aagatgggtc catctatttc
     1801 attgagatga atacgcgcat acaggtggag catccggtga cagaactggt taccggagtg
     1861 gacctgatca aagcgcagat tcgaattgcg gcgggggaac ggttgcagga tgcgaccggg
     1921 gagattcact tttcggggca tgcgattgag tgccggatta atgcggaaga tccggagacg
     1981 tttgtgccat cggcggggcg gatcacaact tttcaagcgc cggggggaac gggagtgcgg
     2041 gtggattcgg cggcgcatgc cgatgcggt
//
LOCUS       DATCBB010000495         1057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_406001, whole genome shotgun sequence.
ACCESSION   DATCBB010000495 DATCBB010000000
VERSION     DATCBB010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406001"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1024)
                     /locus_tag="VII37_05480"
     CDS             complement(149..1024)
                     /locus_tag="VII37_05480"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY5069212.1"
                     /translation="MPRALEQIRRMRGGAQSHLMRCADGHYYVVKFQNNPQHRRVLVN
                     ELLGTRLASRLGLPTAPVASIDVPEELVRLTPDLVMEHPRHRFPCQGGPQFGSRYPGD
                     PRRLALHDFLPDQQLLQVENLHDFAGMLVFDKWTCNTNGRQTLFLADLSSTSGLAMDV
                     AGDGPHSSGGPGGSSVAGSAYRTLMIDQGFCFSAGDWKFPDAPLRGLYARNRVYESIT
                     GMHSFGPWLERLEERITERVLDDISRDIPPEWYEDDYDSLLRLLEQIFRRRTRVPELL
                     LEAKKSNRQPFPNWV"
BASE COUNT          238 a          302 c          314 g          203 t
ORIGIN      
        1 cggtagcgga attgaaaagc aggataccga tgttgaccca ctcatcgcgc accaggttgg
       61 gggtgtagcg cagcacgtga tacgtgcagg ccatctctcc aggggcggcc gcagcgggcg
      121 agtttgagga aggcacatta ggcatggttc acacccagtt cggaaacggt tgacggttcg
      181 attttttggc ctccagcagc agctccggca cacgcgtgcg ccggcgaaaa atctgttcca
      241 acaaacgcag caaagagtcg taatcgtctt cataccactc cggaggaatg tcgcgactga
      301 tgtcgtccag cacccgctcg gtaatgcgtt cttcgaggcg ctccagccag ggcccaaagg
      361 agtgcatgcc ggtaatgctt tcatagacgc gattgcgcgc gtacaacccg cgcagcggag
      421 cgtcgggaaa tttccaatcc ccagcgctaa aacagaatcc ctgatcgatc atcaacgtgc
      481 ggtacgcgct gccggcaacg ctcgaaccac caggcccgcc gctagagtga ggaccgtcgc
      541 cggcaacatc catcgccagg ccactggtgg agctcaaatc cgccaggaac aatgtctggc
      601 ggccattggt gttgcaggtc cacttgtcaa aaacgagcat gcccgcgaag tcgtgcagat
      661 tttctacctg cagcaattgc tggtcgggaa gaaaatcatg cagcgccaat cggcgagggt
      721 cgccgggata gcgagatccg aattgcgggc cgccctgaca gggaaatcga tgtcgcggat
      781 gttccatgac taaatcaggc gtcaagcgaa cgagttcctc gggtacgtct atgctggcca
      841 cgggcgcggt gggcaagccc aagcgcgagg ccaatcttgt gcctagcagt tcattgacca
      901 gaacgcgccg gtgctgaggg ttattttgga acttgactac gtaatagtgg ccatccgcgc
      961 aacgcatcaa gtgtgattgc gcgccgccac gcatccgccg tatctgctcc agtgccctgg
     1021 gcatgcgggt taatgctaac gatagttaat cgtctag
//
LOCUS       DATCBB010000496         1524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_80955, whole genome shotgun sequence.
ACCESSION   DATCBB010000496 DATCBB010000000
VERSION     DATCBB010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80955"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="rocF"
                     /locus_tag="VII37_05485"
     CDS             complement(<1..807)
                     /gene="rocF"
                     /locus_tag="VII37_05485"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY5069213.1"
                     /translation="MGQKVRIIGVPMDLGQSRRGVDMGPSALRVAGLQARLKQLGHQV
                     EDIGNIPVKQPEEMPVGEKRAKYMAEIAETCKDLAEIVQKCLEEDFVPLVLGGDHSIA
                     AGAVTGVAAHFRKEKKQIGYIWLDAHGDMNTPESSPSGNVHGMPLAAVMGYGAPELVD
                     LLGFKPKVEPQNIVLVGVRDLDLQERRLVKKSGVRVFTMRDIDERGMREVMADALKYA
                     TDDTDGISVSLDMDFVDPSDAPGVGTPVRGGVTYREAHLAMEMIADSDAMA"
     gene            complement(832..>1524)
                     /locus_tag="VII37_05490"
     CDS             complement(832..>1524)
                     /locus_tag="VII37_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY5069214.1"
                     /translation="TFGPTFRAEKSKTRRHLTEFWMLEPEAAYAELGDMMNLAEGLVS
                     AVVQSVLKNKSRELEMLKRDTKKLENVKPPFPRISYEEAIGILNKHNNPSKFGDDIGG
                     DEETIVSNEFDRPVMVHRYPASIKAFYMQPDPERPDLALAFDMLAPEGYGEIIGGSQR
                     IHDYDLLVKRLHEYKLPEEPFQWYLDLRRYGSVPHAGFGLGLERTVAWICGTEHIREV
                     IPFPRMIYRVYP"
BASE COUNT          299 a          455 c          406 g          364 t
ORIGIN      
        1 cgccatggcg tcactgtccg caatcatttc catggccagg tgagcctccc ggtacgttac
       61 cccgccgcgc acaggcgtgc ccacacctgg cgcatcggat ggatcgacga agtccatatc
      121 cagactgaca gaaattccat cggtgtcatc ggtcgcgtac ttgagggcgt cggccatcac
      181 ttcgcgcatg cctcgctcat caatatcgcg catggtgaat acgcgcacac cggacttttt
      241 aaccagacgc cgctcctgaa ggtccagatc gcgcacgcca accagaacaa tattttgtgg
      301 ttcaaccttt ggtttgaaac caagcaaatc aaccaactcc ggtgctccgt aacccatcac
      361 cgcagccagc ggcattccgt gcacgttgcc cgacggagac gactccggcg tgttcatgtc
      421 gccgtgagca tccagccaga tgtagccaat ttgttttttc tctttgcgga aatgcgcggc
      481 cacgccagtg acggcgccgg cagcaatgga gtgatcgccg ccaaggacca gcggcacgaa
      541 atcttcttcc aggcatttct gaacaatttc ggccaggtcc ttgcaggttt cggcaatttc
      601 cgccatgtac ttggcccgct tttcacccac cggcatttct tctggttgtt tgacggggat
      661 gttgccgatg tcttcgacct gatggccaag ttgcttcagg cgcgcctgca atcctgcaac
      721 gcgcaaagcc gatggcccca tgtcgacgcc gcggcggctc tgccccaggt ccatgggcac
      781 accgataatt ctgacttttt gccccatata gtgttctttc tttcgaggat actaaggata
      841 tacccggtaa atcatgcgcg gaaagggtat cacttctcgg atgtgctctg tgccgcaaat
      901 ccacgccacg gtgcgttcca ggcctaggcc aaatcccgca tgcggcacgc tgccatagcg
      961 gcgcagatcg agataccact gaaacggttc ttccggcagc ttgtattcgt gtagacgttt
     1021 caccagcaaa tcgtagtcgt ggatgcgctg gctgccaccg ataatttcgc catagccttc
     1081 gggcgccagc atgtcgaacg ccagcgcgag atcggggcgc tcgggatccg gctgcatgta
     1141 aaaagcttta atggatgccg gataacgatg caccatcacc ggccggtcga attcattgga
     1201 gacgatagtc tcttcgtcgc cgccaatgtc gtcgccgaat tttgagggat tgttgtgttt
     1261 gttgagaatg ccgatggctt cctcgtagct gatgcgcgga aaaggcggct tcacgttttc
     1321 cagttttttt gtgtcgcgct tcagcatttc cagttcgcgt gatttgtttt ttagtaccga
     1381 ttgcaccacc gcgctcacca gtccctcggc gaggttcatc atgtcgccca gttcagcgta
     1441 ggcggcctcc ggttcgagca tccagaactc cgtcagatgg cgcctggtct tcgatttttc
     1501 cgcgcgaaac gtggggccga aggt
//
LOCUS       DATCBB010000497         2963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_146659, whole genome shotgun sequence.
ACCESSION   DATCBB010000497 DATCBB010000000
VERSION     DATCBB010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146659"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2632)
                     /locus_tag="VII37_05495"
     CDS             complement(<1..2632)
                     /locus_tag="VII37_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5069215.1"
                     /translation="MALLTGSQLGPYQIVAPLGVGGMGEVYRAHDTRLRREVAIKVVP
                     EATAHDSDRMARFAREAQFLAALNHPNIGSIYGFEESSTMHALVMELVEGPTLADRIA
                     QGPVPIEDALPIMKQITEALEYAHDRGIVHRDLKPANIKIKPDGTVKVLDFGLAKALS
                     DEIKPTEISNSPTLTAAATKAGLILGTAAYMSPEQARGKTVDRRADIWSFGAVAFEML
                     AGKMAFKGDTVSDTLAAVIRGEPDWALLPGNVPNEIQQLIRRCLNKDPRQRLQSIGEA
                     RIAIENVMEGRAAPMRGSGISAAPSSSISGVKENRALKILAGAALAALLVVAGFLLAN
                     MLHPAGTRESPPIRFAILPPPGTSLNSVVRAVAISRDGTRIAFSANGSDGPRLYHRDM
                     NSANAAPISGSEGGVNPSFSPDGRWLAFFANGKLKKISLDGGTPVVIAEGIAYPKGLC
                     WGTDHNIYYAPSYSSPILRISDGGGTPQPATKLQADKGELADFSPDLLPDGKTILFTV
                     FSGGNMDEGTLLAQKIGSNERKVIVAKGADAHFFAPNHLLYMTAGNLMAVEFDPAKLE
                     TTGAPAVVVQDVQAASGAAQFDISDNGALIYVNGGKQAVENSLVVAEHDAKAQPLPVK
                     PNLYESPRFSPDGKLLALTVRLPDPDIWIFDMERGALRRITFAPGEDELPVWSPDGKR
                     IAFASNGRQQAFMVAVDGSGQEEPLMKNDSHFHLQSWSPDGKLIAYERLGTSGQYEIW
                     MLPLGGDRKPYPYLVSQFHVNQPAFSKDGNWLAYTSNESGRAEVYVQRFPGPGEKIQV
                     STDGGNHPVWSHDGKQLVYENAGTLWATEVLVSPFRVGKSRVLYQGDIWNDAAGPNYA
                     LAPGGHRIVVAERIKDPDGGN"
BASE COUNT          642 a          888 c          780 g          653 t
ORIGIN      
        1 cgtttccgcc gtccggatct ttgatccgct ccgccaccac gatgcgatgg ccgccgggcg
       61 caagcgcgta attgggaccc gcggcatcat tccagatatc gccttgataa agaacgcggg
      121 acttcccgac gcgaaacggc gacaccaaga cttccgtggc ccacagcgtg cccgcatttt
      181 cgtacaccaa ttgtttaccg tcgtgcgacc agaccggatg gttccctccg tccgttgaaa
      241 cctgaatttt ttccccggga ccaggaaaac gctgcacgta aacctcagct cggcccgatt
      301 cgttcgaggt atacgccaac caattgccgt ctttcgaaaa cgccggctgg ttcacgtgaa
      361 attgcgaaac cagatacgga tatggcttgc gatcgcctcc cagcggcagc atccatattt
      421 catactgccc cgaagtgccc aagcgttcat aagcaatcag tttcccgtcc ggcgaccacg
      481 attgcaggtg aaaatgtgaa tcattcttca tcagcggctc ttcctgtccg ctgccgtcca
      541 cagcaaccat gaaagcctgc tgcctgccat tggaggcaaa agcgattcgc tttccatccg
      601 gagaccagac cggcagctca tcctcacccg gcgcgaacgt gattcgacgc aatgcgccgc
      661 gctccatgtc aaagatccag atgtccggat ccggcaggcg caccgtcagc gccagtagct
      721 tcccatcagg agagaaccgc ggagattcat aaagattcgg ttttaccggc aacggctggg
      781 ccttggcatc gtgctctgcc acgaccaggc tgttttccac cgcctgcttc cccccattca
      841 cataaatcag cgcgccattg tctgatatat cgaattgcgc cgcaccgctc gcagcttgca
      901 catcttgaac caccaccgct ggcgcgcccg tggtctcgag cttggcggga tcaaactcga
      961 cagccatcaa attaccggcg gtcatataca acaaatgatt aggtgcaaaa aaatgcgcgt
     1021 cggcgccttt agcaacgata accttccgtt cattcgaccc gatcttctgc gccaatagtg
     1081 ttccttcatc catattgccg cccgaaaaaa ctgtgaacag tatggttttg ccatccggta
     1141 aaagatccgg agagaagtcc gccaactcac ctttatccgc ctgcagcttt gtggcgggct
     1201 gcggcgtccc accgccgtca ctgatacgca gaattggtga gctgtaagag ggcgcgtagt
     1261 aaatattgtg atccgtcccc caacatagtc ctttcgggta agcgattcct tctgcaatca
     1321 ccaccggagt gccgccatcg agcgagatct tttttaattt tccgtttgca aaaaaagcca
     1381 gccatctgcc atcaggcgaa aacgatggat ttaccccgcc ttcggaaccg ctgattggag
     1441 cggcgttcgc agaattcata tcccggtgat acaaccgcgg gccgtccgag ccgttggcac
     1501 tgaaagcgat gcgagtgccg tcacgcgaga tcgccactgc acgcacaaca gaattgagag
     1561 aagtgcccgg cggcggcaga attgcgaagc gtatcggtgg actctcgcgc gtgcctgccg
     1621 ggtgcaacat gttagccaat aaaaagcccg ctacaaccag caatgccgcc aacgcagcgc
     1681 cggccagtat ttttagtgcc cgattttctt tcaccccgga gatagaggaa ctcggtgccg
     1741 cgctgatgcc ggaacctctc attggcgcgg cccggccttc catcacgttt tcgatggcaa
     1801 tgcgcgcttc gccgatggat tgcaaccgct gccgtggatc tttattcagg cagcgccgga
     1861 tcagttgttg aatctcattt ggcacgttac ccggaagtaa ggcccaatcc ggttcaccgc
     1921 gaatgaccgc agccaaagtg tcggagacgg tatcgccttt aaaagccatt ttgcctgcaa
     1981 gcatctcgaa cgcgaccgcg ccgaaagacc agatgtccgc gcggcggtcc accgtttttc
     2041 cgcgagcttg ttccggcgac atgtatgcgg cagtgccaag aatcaatccc gctttggttg
     2101 ccgctgcggt cagtgtcggg gaattcgaga tttcggtagg cttgatttca tcggacaggg
     2161 ctttcgctaa gccgaagtcc aaaactttga ccgtgccatc aggcttgatt tttatgttcg
     2221 cgggcttcaa atcgcggtgc acgatgcccc ggtcatgagc atattcgagc gcttcggtaa
     2281 tttgcttcat gatcggaagc gcgtcttcga tgggtacggg gccttgagcg atgcggtcgg
     2341 cgagtgtcgg gccctccacc aactccataa ccaacgcgtg cattgtggaa gattcttcga
     2401 acccataaat gctgccgata ttcgggtgat tcaaagcagc aagaaattgc gcctcgcgag
     2461 cgaagcgtgc catgcggtct gagtcgtgcg cggtggcctc gggaactacc ttgatggcta
     2521 cttcgcgacg cagccgcgta tcgtgcgcgc gatatacctc gcccattccg cccacgccga
     2581 gtggtgcgac aatttgataa gggccaagtt gtgagccagt cagaagagcc atgcgcggcc
     2641 agattatact gcgcttcgcg tcaatttcac cgcgggttca gaacttttga atcgatgcgg
     2701 tcgacccata aacgacgaca cgggctgttt tggaaatgtg gacacgaata ttctggtcat
     2761 ctcaagaact gaaggaaatg cacccgtcct caggtgagcg ctggatggtg ggtcagtttc
     2821 cgagtaccgc gtttaccatg cactgagaac ctcggctgca tcgtctcgtc gcgctcaaat
     2881 tcctgcccga cgatgttgcc atggaccccc agtccttggc ccgttttcaa cgcgaagcac
     2941 aggcggcctc tgccctgaat cat
//
LOCUS       DATCBB010000498         1136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_114414, whole genome shotgun sequence.
ACCESSION   DATCBB010000498 DATCBB010000000
VERSION     DATCBB010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114414"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..578)
                     /locus_tag="VII37_05500"
     CDS             complement(180..578)
                     /locus_tag="VII37_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069216.1"
                     /translation="MNSKNKITLRLLAFCLALAMGIAPALAKPQASSSDSNPAAAKPA
                     AGNSGATSASQSAPVSSANGAPKASGKSAGGAASDSEIAAAKASGKVWVNTESRVYHK
                     GGRWYGKTKAGKFMTEPEAKAAGYKAAHKE"
     gene            complement(603..>1136)
                     /locus_tag="VII37_05505"
     CDS             complement(603..>1136)
                     /locus_tag="VII37_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEY5069217.1"
                     /translation="ILEVGGGLYNKNGINVENLWEFRQRNGTIHGFTGAEKYDPAELL
                     LTDCEILIPAATENQITSQNADRVKCKILAEGANGPTTAAADDILADKKVFIIPDILA
                     NAGGVTTSYFEWVQDRQGYFWKESEVNDRLGDIMVTAFDDVVRYAETHGVNNRIAAYM
                     LAIDRVAYTIRQRGIYA"
BASE COUNT          229 a          319 c          303 g          285 t
ORIGIN      
        1 tttgctggcg gagttgatgt cgagcagatc ggcgctggtt tttacggttg ggcttttggc
       61 ggcagatgtg gacgattggg ccacagcgac gccggtcatc aagctaagta taaaaattgc
      121 ggtgaaaatt ctgttcatga actttgcgga catcgtcatt gttagctttc cttgaattct
      181 tattctttgt gagctgcttt atagccggct gccttcgctt ccggttcggt cataaacttt
      241 ccagctttcg tcttgccata ccagcggccg cccttgtgat acacccggct ctcggtgtta
      301 acccagactt tgccgctggc cttggcggcc gcgatctccg aatcggaggc cgcgcctcca
      361 gcagacttgc cggaagcttt aggcgcacca ttggcggacg aaactggcgc cgactgacta
      421 gctgacgtcg cgccggaatt gcccgctgca ggctttgcgg cagctggatt actgtcgctt
      481 gagctagcct gcggtttcgc caaagcaggc gcaatgccca tcgcaagcgc caggcaaaag
      541 gccagcagcc ggagagtaat tttattcttg gaattcatac ttcctcccca gatcaacgaa
      601 agctaggcat aaattccgcg ttggcgaatc gtataggcga cgcgatcgat agccagcata
      661 taagcagcga tgcggttatt cacgccatgc gtttcagcgt accggacaac gtcgtcgaac
      721 gcggtaacca taatgtcacc cagacgatca ttgacctccg actctttcca gaaatatccc
      781 tggcgatcct gcacccactc aaagtaggag gtagtaactc caccggcgtt tgccagaatg
      841 tcgggaatta tgaaaacttt cttgtcggcg aggatgtcgt cggctgccgc ggtggttggc
      901 ccattcgcgc cctcggcaag tattttgcac ttcacccggt ccgcattctg gctggtgatc
      961 tggttttccg tcgccgccgg aatcaaaatc tcgcaatccg tcagcaagag ctccgctgga
     1021 tcgtatttct ccgccccggt gaatccgtga atggtgccat tgcgctggcg gaactcccac
     1081 agattttcga cattgattcc attcttgttg tagaggccgc ccccaacttc gaggat
//
LOCUS       DATCBB010000499         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_146681, whole genome shotgun sequence.
ACCESSION   DATCBB010000499 DATCBB010000000
VERSION     DATCBB010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146681"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VII37_05510"
     CDS             <1..1512
                     /locus_tag="VII37_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069218.1"
                     /translation="ARNSAQEKLNAGKPRLMLASFSASPEPEPQRPGGGAGAGSGPAG
                     GRGTGGSGSTGGTGGTGAKGGTGGTSGGTTGGAGGKGSTGGTSGGTGTGAGRGSGSSG
                     GVANPGAYNFNSPYNQPRTIVPPFPQSASTNQQILAMLAEGTGGFTIFNTNDLLGGLQ
                     RIGREQSEFYVLGYVPTDTPEGSCHTLKVKLSRGGTNVRSRSGYCNVRTGNTLEGKPL
                     EKQLESRATGAQGGSIHGGMQAPYFYTSANVARVNLAMEIPADAVHFNKEKGKYSANL
                     NVLGIAYRTDGSVGARFSDTVNLNLEKDEWKQFTQNPYRYENQFDATPGTYKLTVVLS
                     AGGDAFGKFEAPLQIDTYDGKHFSLGGVALTDSVQKLGDIPTSLDSALLEDRTPLVVQ
                     GLQILPSGTNRFKRDQNVVVYSEIYEPLLTSDSPPKIGMGYRILDRATKKEVMFTGVV
                     AADAFIKKGNPVVPVGLKVNVKDLNPGSYSLVLQAVDGAGNKAPNRTVDFDVL"
     gene            1795..>2250
                     /locus_tag="VII37_05515"
     CDS             1795..>2250
                     /locus_tag="VII37_05515"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY5069219.1"
                     /translation="MKPSHAKLLSRDSRKQEATVSLRPKALLAAFLSIACVLSLGVDV
                     PAIAAIPAAAAKDKKPKQVPALKGLPITELTADEAILHAMNRLAYGPRPGDVERVRQM
                     GLAKWIDQQLAPNSIDDKALNARLENLPTLSMSAAQLIEEYPQPKQAAAQ"
BASE COUNT          538 a          655 c          591 g          466 t
ORIGIN      
        1 gcaagaaaca gcgcacagga aaaattgaat gctggaaaac cacgtttgat gctggcgtcc
       61 ttttccgcct cacctgaacc cgaaccgcaa cggcccggcg gcggtgccgg agccggcagt
      121 ggacctgccg gaggacgcgg cacgggcggc tcaggtagta ccggaggaac cggtggaact
      181 ggcgccaagg gcggcaccgg cggaacttca ggcggcacaa ccggcggtgc cggcgggaag
      241 ggaagcactg gcggaacttc aggcggcact ggcacaggcg caggacgcgg ctccggtagc
      301 tcaggtggag ttgctaaccc gggcgcttac aattttaatt cgccgtacaa ccagccgcgc
      361 accatcgttc caccatttcc gcagtcggcg tccaccaatc agcaaattct ggcgatgtta
      421 gcggaaggca ctggtggatt tacgatcttc aacaccaacg atctcctcgg tggtctgcag
      481 cgtatcggac gagagcaaag tgaattttat gttctcggtt atgtgccgac cgacacgccg
      541 gaaggctcat gccacacgct taaagtgaag ttaagccgcg gcggaacgaa tgtgcgttca
      601 cgaagcggat attgcaacgt gcgaaccggc aacactctcg aaggaaaacc cctagaaaaa
      661 caactcgagt cgcgcgctac tggagcgcaa ggcggatcga tacacggtgg catgcaggct
      721 ccctattttt acacctccgc aaatgttgcg cgcgtgaatc tggcgatgga aattcccgcc
      781 gacgccgtgc atttcaataa agaaaaagga aaatatagcg ccaacttgaa tgtgttgggc
      841 atcgcctatc gcaccgatgg atctgtcggc gcgcgcttca gtgacaccgt gaatctcaat
      901 ctggaaaagg atgaatggaa gcaattcacc cagaatccct atcgttacga aaaccagttt
      961 gatgccactc ccggaaccta caaactgacc gtggtgctaa gcgccggcgg cgacgccttt
     1021 gggaaatttg aagcgccgct gcaaattgat acttacgatg gcaaacactt tagcctcggc
     1081 ggtgtggcgc ttaccgatag cgttcaaaaa ctcggcgaca ttcccaccag ccttgattct
     1141 gcgctactcg aagaccgcac tcccttggtg gtccagggct tgcagatttt gccatccggt
     1201 accaaccgct tcaaacgcga ccaaaacgtg gttgtatatt ctgagattta cgagcccttg
     1261 ctgacttccg atagcccgcc gaagatcggc atgggttacc gtattcttga tcgcgccacc
     1321 aagaaggaag taatgttcac cggagtcgtg gctgccgatg ccttcattaa aaaaggcaat
     1381 ccagtagtgc cggtgggctt gaaggtcaac gtcaaggacc tgaatccagg ctcatactcc
     1441 ctagtgctgc aggccgtcga cggagccggc aacaaagctc ccaatcgcac cgtcgacttc
     1501 gacgtgttgt aatttttcac taacaatact tatcgcgatt tatcgacgtg ttgtaatcat
     1561 ttccctatta ctccgttacc tcaaaaaagg cggagggtgc cccaccctcg gatttttgga
     1621 gggtgggtct tgtaacgtgg aaacatcgtt caactaaacc gcgaagctgt tctcagcgtc
     1681 gcctccgctc aacacccatt ccgtagcttt tttccggaat cgccgcattc gacacatcgc
     1741 cacgcaccct gcaacttccc gccacgtcta gggttaatct tgtatggagc aggcatgaag
     1801 cccagccacg caaaactgct atcgcgtgat tcccgcaagc aagaggctac cgtgtctctg
     1861 cggcccaagg ccctcctcgc ggcatttttg tcgattgcct gcgtattgtc cttaggagtt
     1921 gatgttcccg ccattgctgc gattcccgca gcagctgcca aagacaaaaa acctaaacaa
     1981 gttcccgcat tgaaaggttt gcccatcacg gagctcaccg ccgatgaagc cattcttcac
     2041 gccatgaacc gtctcgccta tgggccgcga ccaggcgatg tcgagcgcgt gcgccaaatg
     2101 ggattggcta agtggatcga tcaacagctc gcgccaaatt ccattgacga taaagctctc
     2161 aacgcgcgcc tcgagaatct ccccacgctc tccatgtccg ctgctcaatt gatcgaagaa
     2221 tatccgcagc ccaaacaagc cgcggctcag
//
LOCUS       DATCBB010000500         1118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_210804, whole genome shotgun sequence.
ACCESSION   DATCBB010000500 DATCBB010000000
VERSION     DATCBB010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210804"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VII37_05520"
     CDS             complement(<1..392)
                     /locus_tag="VII37_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069220.1"
                     /translation="MPVGTAFHERTFALCESLNYREWSGYYTVSVYEMHHEHEYNAIR
                     NSAALIDISPLFKYKVTGRDATKLVNRVISRDINKVAVDQVIYCCWCDPEGKVIDDGT
                     ITRLGENEYRWTAADPSLRWFQQNALGL"
     gene            complement(506..>1118)
                     /locus_tag="VII37_05525"
     CDS             complement(506..>1118)
                     /locus_tag="VII37_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5069221.1"
                     /translation="EVTWHGQLVTTGIFKEPIAGRVALRKLNLDGDRQADLTVHGGKY
                     KAVYCYPLEHYAYWKKELPGRDLPLAIFGENLTTEGMLEDSVQLGDRFSVGTAEVVVT
                     QPRLPCYKLGIRFQSDDMVRRFLASRRSGFYVAVTREGEVGAGDKIKEISRDKNQVSI
                     ADIVRLFVTKRFTGADAELVRRAAQVAAFPENWKEEFAERLAG"
BASE COUNT          240 a          377 c          258 g          243 t
ORIGIN      
        1 tccagcccca acgcgttctg ctgaaaccat cgcaagctgg gatccgccgc cgtccagcga
       61 tattcgtttt cgcccagccg cgtaatcgtg ccgtcgtcga tcactttgcc ttcgggatcg
      121 caccagcagc agtaaatcac ctggtcgacg gccaccttat tgatgtcccg cgaaatcacg
      181 cggttcacca gtttggtggc atcccgcccg gtcactttgt atttgaacag cggagaaata
      241 tcgatcagcg ccgcggaatt gcggatggcg ttgtactcgt gctcgtggtg catctcatac
      301 acgctcaccg tgtagtagcc cgaccactcc cgataattca ggctctcgca cagcgcaaac
      361 gttctttcgt gaaacgcggt accaaccggc acaagccctc caatattcgc agccacctgc
      421 ggcgaaaaca aaacccgtta caacgccatc gaattatagg tgcgaattcg ccgcgctcgc
      481 aaggcgcgca atcacggcat ggaaattatc ctgccagccg ctcggcaaac tcttccttcc
      541 agttctccgg gaatgccgcc acctgcgcag cccgccggac tagctccgcg tcagcgcctg
      601 taaatctctt ggtgacaaat aaccgaacga tatccgcgat tgatacctga ttcttatcgc
      661 gcgatatttc ttttatctta tctcccgcgc caacttcgcc ttcccgcgtc accgccacat
      721 aaaatccact acgccggctg gccagaaatc gccgcaccat gtcgtcggat tgaaacctga
      781 tgcccagctt gtagcaaggg agccgcggct gcgtcaccac cacttccgcg gtccccactg
      841 aaaatcgatc gcccagctga acggaatctt ccagcattcc ctcggtcgtt aaattttctc
      901 caaaaattgc cagcggcaaa tcgcgcccgg gcaattcctt cttccaatac gcgtagtgct
      961 ccagcggata gcaatacacc gccttatatt tgccgccgtg cacggtcagg tcggcctggc
     1021 ggtccccatc gagattcaat ttccgcaaag ccacgcggcc cgcaatcggc tccttgaaaa
     1081 tcccggtcgt gaccagttgc ccatgccagg tcacctct
//
LOCUS       DATCBB010000501         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_146720, whole genome shotgun sequence.
ACCESSION   DATCBB010000501 DATCBB010000000
VERSION     DATCBB010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146720"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VII37_05530"
     CDS             complement(<1..1115)
                     /locus_tag="VII37_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5069222.1"
                     /translation="MSIPRKARLEANPWKSTPDVLKDARENYVDRCAVCHGVDGSGQT
                     QVGSNLYPKVPDLRSPRTQRLTDGEIRYIIRNGVRLTGMPGWGKPHDEQSDDSWKLVL
                     FIRSLGILSKGEQKEQISGANSAHYTGSKSCEKCHAEIYERWRKTPMANVVRDPREHP
                     DAIIPDLATNTIAKFTKEDVALVYGSVWKQRYFTKRGDDYFPEPAQWDITGHVWRKYF
                     VAKGTDWWEPFYPADNMQRPTGTTCDGCHSVDYNIHTKQVAEWNVGCEKCHGPGSEHV
                     EHPSRTNILNPAQMDYVQASDTCIQCHSQGRPPVSPIEGKYYDWPVGYQVGLHLQDFW
                     QLEEHTLGETTFTHFPDGTAHKNRMQGNDFVQSVMYRR"
     gene            complement(1293..4019)
                     /locus_tag="VII37_05535"
     CDS             complement(1293..4019)
                     /locus_tag="VII37_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5069223.1"
                     /translation="MIGQTIAHYRIVEKLGGGGMGVVYKAEDTRLGRFVALKFLPENV
                     AQDPLSLERLRREARAASALNHPNICTIYEINESESQTFIAMELLEGSTLKHLIAGRP
                     MEMETVLELGIQIAAALEVAHSKGILHRDIKPANLFVTNHGQAKVLDFGLAKLTRDEN
                     AGATSAPTITEENLTSPGVAIGTVAYMSPEQVRGKELDARTDLFSFGAVLYEMCTGSL
                     PFQGETSGVIFEAILNRAPVAPVRLNSRVPARLEEIINKTLEKDREVRCQSAAELKAD
                     LKRLHRDSISGTAAHVTPAVASALPSKKWQIGGAIAATLVIVSALAIWFAWHRGSASK
                     GPQATLTARRLTSNPIENPIAVSAISPDGKYLAYSDHTGTYLRLMATGEVHALLPKNN
                     DVQYLSWYPDSTRLLLSWPPSPAAKMGLWVLPIVGGTPRQLSEEGWSASVSADGSQIV
                     FLKAAMFGETGAELWVMRADGSEQRRIISADEDGTAYSSPVWSPDGHWIAYDKFRFGA
                     YTNEAWVELFNPEHATRNVVITEPQLEWGVEWLPDGRLLYAIAEPPPSQNSSNFWAVN
                     VNPTTGRSSGAPVKITNGDDHVNQPSITGDGIKLVFSRYQPQLDVYVAEFFAKGPHLG
                     TPRRLTLDNSDDLPFDWTFDDSSVLFTSNRTNAGSIFNIFQQRIDQSSAEMLVSGPEQ
                     KAVTRLSADGSEILYLESLSSQDTGGQRRSETQQDSRPVRLLRAPAQGGPSQLVLEAP
                     NIINFQCSRRGTGVCVLSQAEPKDYPFSVFDPMKGTLRQAAKLEEAPSGWNWGLSPDG
                     TAIAAVQVGANQHQIRLVSLTGQPARVISVKDWNNFMSLDWAADGKGFFISSNPTGRL
                     DTVLYVDMAGKATPLWQVKNFQAAWVIPSHNGKYVAMPAPTTECNVWMVENF"
     gene            complement(4444..>4998)
                     /locus_tag="VII37_05540"
     CDS             complement(4444..>4998)
                     /locus_tag="VII37_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY5069224.1"
                     /translation="CAADETGEAEDGAFEGWTARTLHESKVVQGMPRKLTSDEHLDYV
                     AALLDDIFQIPGTKIRFGLDALIGWEPGIGDAMAGTASFLIVFASWQRGARAITLTRM
                     IANVLLETMVGAIPVAGDIFHVVWKANRRNYRLLMREREQPGFHTGRDWMFLVVILII
                     AIAAVGIPIGILIWILRLHPVVRT"
BASE COUNT         1096 a         1539 c         1242 g         1121 t
ORIGIN      
        1 cggcggtaca tcacactctg cacaaaatca ttcccctgca tgcggttctt gtgcgccgtg
       61 ccgtcgggaa agtgcgtgaa agtcgtctca cccagcgtgt gttcttccag ttgccaaaaa
      121 tcctgcaaat gtaaacccac ctgatagcca actggccaat cataatattt gccttcgatg
      181 gggctcaccg gcgggcgtcc ttgcgaatgg cactgaatgc atgtatcgct ggcctgaaca
      241 taatccattt gcgctggatt cagaatattt gttcgcgacg gatgctccac gtgttcgctt
      301 ccaggcccgt ggcatttctc gcaccccacg ttccactccg cgacttgttt cgtgtggatg
      361 ttgtagtcca cagaatggca cccatcacac gtcgtgcctg tagggcgctg catattgtcc
      421 gccggataaa atggctccca ccaatccgtt cccttggcca cgaaatattt ccgccaaaca
      481 tgcccggtaa tgtcccactg cgccggctcc ggaaaataat catccccgcg tttcgtgaag
      541 taacgctgct tccaaacgct gccgtaaacc agcgcaacat cctcctttgt gaacttggca
      601 atagtattcg tcgcaagatc aggaatgatc gcgtccggat gttcccgggg atcccgcacc
      661 acattcgcca tcggcgtctt gcgccagcgc tcgtaaattt ccgcgtgaca tttctcgcag
      721 gattttgacc ccgtgtagtg agccgaattt gccccggaaa tctgctcttt ttgttcgccc
      781 ttgcttaaaa tccccaggct gcgaatgaac agcaccagtt tccagctatc atcgctctgc
      841 tcgtcgtgcg gtttccccca cccgggcata cccgttaatc gcacgccatt gcgaatgatg
      901 tagcggattt ccccatccgt cagtcgctgc gttcgcggcg agcgcaagtc cggcactttg
      961 ggataaagat tgcttcccac ctgcgtctgc cccgagccat ccactccgtg gcaaaccgcg
     1021 caacgatcca cgtaattttc tcgcgcgtcc ttcagaacat ccggcgtact cttccacgga
     1081 ttcgcttcca gtcgcgcctt ccgcggaatc gacaaattcc gcgccgtccg cgccacaaca
     1141 gtttctaaaa atgacggctg gtccgcggta ctaaacccac gccaaaccaa ccgtattcca
     1201 taaaagccaa gcgcaatcag gatcacgccg cacagtgaaa gaaaaaaatt tcgtgagttc
     1261 atacggaaag tatttcagct accaagcact gctcaaaaat tttctaccat ccaaacattg
     1321 cactccgtcg tcggcgctgg catagccacg tacttcccgt tgtgcgaagg aatcacccaa
     1381 gccgcctgga agtttttcac ttgccacagc ggcgtcgcct tcccagccat gtccacgtac
     1441 agcaccgtgt ccaggcgtcc ggtaggattg ctggagataa aaaatccttt gccgtccgcc
     1501 gcccagtcaa gcgacatgaa attgttccaa tcttttacgc tgatcacccg cgcgggctgg
     1561 cctgtcagcg ataccaatcg aatctgatgc tggttggcgc ctacttggac tgcggcaata
     1621 gcagttccat ctggagaaag gccccaattc cagccgctcg gagcttcttc cagcttggcg
     1681 gcctgacgga gcgtgccctt catgggatca aatactgaaa agggatagtc cttcggttct
     1741 gcttggctta acacgcacac gcccgtgccc cttcgcgaac attggaaatt aatgatgttg
     1801 ggagcttcca gaacaagttg tgaaggacct ccctgggctg gtgcgcgcaa cagacgcacg
     1861 ggtcgggaat cttgctgggt ctcggaccgg cgttgcccac cggtgtcttg cgaactcaac
     1921 gattccaagt aaagaatctc agatccgtca gcactcagcc gcgtaacggc tttttgttct
     1981 ggacctgaca ccagcatttc cgcggagctt tgatcaattc gctgctggaa aatgttaaag
     2041 atgctacccg cgttcgttct atttgaagtg aacagtacgg aactatcatc aaacgtccaa
     2101 tcgaaaggca ggtcgtccga gttgtcaagc gtaaggcggc gaggcgttcc gaggtgtgga
     2161 cccttggcga aaaattccgc cacgtaaaca tccagttgtg gctggtagcg gctaaataca
     2221 agttttatcc cgtcgccagt tatgctcggc tgatttacat ggtcgtcgcc gttggtaatc
     2281 ttcacaggag cccccgagga acgtcccgtg gtcgggttca cattaactgc ccagaaattt
     2341 gaactgttct ggcttggcgg cggttcggct atggcataaa gcaaccgccc gtcaggcagc
     2401 cattccactc cccactccag ttgcggctcg gtaatgacta cgtttcgggt tgcgtgctcc
     2461 gggttgaaaa gctcgaccca cgcttcattg gtataggctc caaagcggaa cttgtcatac
     2521 gcgatccagt gtccatccgg ggaccacacc ggtgacgagt aggcggtacc atcttcgtct
     2581 gcggaaatta tcctgcgttg ctcgcttccg tcggcccgca tcacccacag ttccgcgccg
     2641 gtctcgccaa acatcgcggc cttgagaaat actatttgtg agccatccgc agacaccgaa
     2701 gcggaccaac cctcttcaga aagctgccgc ggggttcccc ccacaatggg cagcacccat
     2761 aagcccatct ttgcggctgg cgacggcggc cacgaaagca acaaccgcgt gctgtccgga
     2821 taccaactca ggtattgaac gtcgttattc tttggcaata aagcgtgcac ttcccctgtg
     2881 gccatcagtc gcaagtaagt gccagtgtgg tcggaatacg ccaggtactt tccatcagga
     2941 gaaatcgccg agacagcgat aggattttcg atgggattgg aagttaagcg tctcgcagtt
     3001 aatgtcgctt gcgggccttt cgatgcgctt ccacgatgcc aggcaaacca tatcgctagt
     3061 gcgctaacga tgaccaatgt tgcggcgatg gcacccccta tttgccattt ctttgaagga
     3121 agtgcgctgg ccaccgccgg tgtcacgtgc gctgcggttc cggagatgga atcccgatgc
     3181 aagcgcttca agtccgcttt cagttccgca gccgactggc agcgcacctc gcgatctttc
     3241 tccagcgtct tattgataat ttcttccagg cgcgcgggca cgcgcgaatt caaacgcacc
     3301 ggcgcaactg gcgcgcgatt taaaatcgct tcaaaaatca ctcccgaagt ttcgccctga
     3361 aaaggcagcg acccagtgca catctcataa agcaccgcgc caaacgaaaa taaatccgtg
     3421 cgcgcatcca gttcttttcc gcgcacctgc tccgggctca tgtacgctac ggtgccgatg
     3481 gccacgcccg gactggtcaa attttcttcg gtgatcgttg gcgccgaagt ggctcccgcg
     3541 ttttcgtcgc gggtcagctt cgccagcccg aaatccagca ccttggcttg cccgtggtta
     3601 gtaacaaaaa ggttcgccgg cttgatgtcc cggtgcagga tgcccttgct gtgggccact
     3661 tccagcgccg ccgcaatctg gatccccagc tccagtaccg tctccatttc catcggccgc
     3721 ccggcaatca aatgcttgag agtactgcct tccagcaact ccatggcgat gaaggtctgg
     3781 ctctcactct cgttaatttc ataaatcgtg cagatgttcg gatgattcag agcggacgcc
     3841 gcgcgcgctt cgcgccgcaa tcgttccagg ctcagcggat cttgcgcgac attttccggc
     3901 aggaatttca gagcaacgaa ccgccccagg cgcgtatcct cagccttgta caccacgccc
     3961 atgccgccgc cgcccaactt ctctacaatt cggtaatgag caatggtttg gccaatcatg
     4021 gcttacggat agcgctgaca tattaggagc gcgacctact caagtcaatt catcagcgga
     4081 tttcttcggc cgggaagatg ccacctacat ccacacgtgc cgcgctataa atacccgccg
     4141 ctcgccaaag tccatggctg catgcaagct cccatcagca gcggacagca gccaaagcgc
     4201 cgaatttatc gttcaaagtc agtgccaacc atttgttatt gggtgaaaac accgggtgca
     4261 actcctgcca cgccggaaca cgcgatcccg caattgtggc cagcagggaa gtaggcccgt
     4321 tttccgggcg cgctcgtcgt atttcataat ccgataatcc gttcactgtc tgcagcgtct
     4381 tgccacaaaa aaaaggcgcc gtcagaggac ggagccgcag ccaaagcctt aaccgcagcg
     4441 cattcatgtc cggacaaccg ggtgcaagcg caatatccag atcaaaatcc caatcggaat
     4501 ccccacggca gcaatcgcta taattaaaat gacgaccagg aacatccaat cacgcccggt
     4561 gtgaaatccc ggttgttccc tctcgcgcat cagtaaccgg tagttccgtc ggtttgcctt
     4621 ccaaacgacg tggaaaatat cgcccgcaac cggaatcgct cccaccatcg tttccagaag
     4681 cacattggcg atcatgcgcg ttaaagtgat ggcgcgcgcg ccgcgctgcc aggaagcaaa
     4741 cacaatcaaa aacgaagccg tccctgccat cgcgtcgccg attcccggtt cccacccgat
     4801 cagtgcatca agtccgaatc gaatcttggt ccccgggatt tgaaatatgt cgtccagcag
     4861 cgcagcaacg taatccaggt gctcgtctga ggtgagtttg cgcggcatgc cctgaaccac
     4921 tttactctca tgcagcgtcc gtgcagtcca gccctcgaaa gcgccgtctt ccgcctcacc
     4981 ggtttcgtct gccgcgca
//
LOCUS       DATCBB010000502         1026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_275908, whole genome shotgun sequence.
ACCESSION   DATCBB010000502 DATCBB010000000
VERSION     DATCBB010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275908"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(741..920)
                     /locus_tag="VII37_05545"
     CDS             complement(741..920)
                     /locus_tag="VII37_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069225.1"
                     /translation="MTADESKDLKKGTRVCWQGDAADRGTITQTSWDAVTITWDNGHV
                     AIVHHGDMREIQRAK"
BASE COUNT          224 a          315 c          249 g          238 t
ORIGIN      
        1 aatgtgatgg caaggccgag cacgccggcg aaagcgacaa ttcgccagtc gctatacctc
       61 tccacatctt tctgtgcttg acgcattgct gcacactcgg gctgcttgcg gcgcacgccc
      121 aacaatccgc gattttcaac aacttatagg gactggttgg ggagccacgt taatttccgc
      181 gagccacaat cgctaattgg caattagtga tacgaagata tgaacgcgat cgtgacattt
      241 tgcgcgaaaa tcgcggtacg gcagcctttc aaggcggctg caggaaagat aaataacggc
      301 tctttgaact ttcaggctcg gcagcaatgc ctacgcctgc aactcagccc cgccactcac
      361 gtgcgcggga ctttttcgtt cccctatccc caccagaaat gaattgcctc aaaaacgata
      421 agaccgatca gtactccgca aaatagtgcg gctgccatgg gccagcgcag cgtgcgccgt
      481 cgttgttccg atttgatata cgtcgaaata tgccgcacac cggcagtcta acatttgacg
      541 tgatgtcgcc gcgccacaca ttacaagaat gtcaagccgc agcattcgtt ccccggcact
      601 gcctccgcaa tgtccgcagt gccgtccgct cgttcgcgag cggacttatt cgcttctctg
      661 catatccctg ccgcgtgtta caccgcggcc gctcacttgt gagcggacta caagtcactg
      721 ctccatgttg tagagctgct tcacttcgct cgctggattt cgcgcatgtc cccgtggtgc
      781 acgatagcca catgaccatt gtcccaggtg atcgtgaccg catcccaact cgtttgagtg
      841 atagtgcccc tgtcagcagc atcaccctgc cagcaaacgc gagtaccctt cttgaggtct
      901 ttagattcgt cggcggtcac gtggcgcctc cttttgcatt agtcgcgcag aaccaacctc
      961 gaagatattc tcactcctcg tcattaggcg tatgatccat acagcttaac aggggcgccc
     1021 catgac
//
LOCUS       DATCBB010000503         1169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_242817, whole genome shotgun sequence.
ACCESSION   DATCBB010000503 DATCBB010000000
VERSION     DATCBB010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242817"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VII37_05550"
     CDS             <1..1103
                     /locus_tag="VII37_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5069226.1"
                     /translation="FFAGYFCFQLPSNLVLAQIGAPRWIALLMVLWGIVSASTVFVTT
                     PRSFYLLRFLLGAAEAGFFPGIIFYFKNWFPASVRARTIAWFMTAGPLSGAIGGPISG
                     ALLGFPQTGRLAGWQWLFLMEGLPAILLGIVVFFYLTASVETAPWLSPEQRKWLMDTL
                     RSERQLLAAAGGANAFAAFRSGKVWLLAFVYFGLNTCVYGISLWLPTLIRNLSGMSSF
                     AIGLISAIPYLAAATAMVLVGHHSDHSGERRWHIAIAAFVGAAALGLTAYSQSAVPLI
                     VATSFAIAGASAMDGPFWAMSTSLLSGTAAAAGIALINSVGNLGGFFGPYIIGLVRTS
                     TGGFKGGLLVSGATLAASGCLALLVRSRRVTA"
BASE COUNT          198 a          292 c          354 g          325 t
ORIGIN      
        1 tgttcttcgc tggatatttc tgtttccagt tgccaagcaa tctggtgttg gcccagattg
       61 gcgcgccacg ctggatcgca ctgctgatgg tgttgtgggg aattgtttcg gcctcaacgg
      121 tattcgttac cacgcctcga agtttttatc tgctgcgctt tttgctgggg gcggcggaag
      181 cgggtttctt tcccggaatc atcttctatt tcaagaactg gttccccgcg tcggtgcggg
      241 cgcgcacgat cgcgtggttt atgacagcag gtcctttgtc tggagctatt ggcggtccaa
      301 tttctggagc gctgctcggt tttcctcaga ccggccggct tgcaggatgg cagtggctct
      361 ttctaatgga gggactgccg gcaatccttc tcgggatcgt tgtttttttc tatttaaccg
      421 ccagtgtgga gaccgcccca tggctgtcgc ctgagcagcg aaagtggctc atggatactt
      481 tgcggagcga acgccaattg cttgccgctg ccggcggggc aaatgctttt gccgcattcc
      541 gcagtggcaa ggtttggctt ttggcatttg tttatttcgg cttaaacact tgcgtatatg
      601 gaatctcgct ctggctgcct accctgattc gtaatttgtc aggaatgagc agtttcgcca
      661 ttgggctgat ttcggcgatt ccgtatctcg cagctgcgac tgcaatggtt ttggtggggc
      721 accattccga tcacagcgga gagagacgct ggcatatagc catcgcagct ttcgtgggcg
      781 ccgcagcctt gggacttacg gcttattcgc aatccgcggt gccgctgatt gtggccacga
      841 gttttgcgat tgcaggtgca tccgccatgg acggcccctt ctgggcgatg tcaacctctt
      901 tgctttcggg aacagccgcg gccgctggaa ttgcgttgat caattcagtg ggtaatctgg
      961 gtggattctt tggcccttat attatcgggc tcgtccggac gtctaccggc ggcttcaaag
     1021 gaggattact cgtaagtgga gcgacgctgg cagcgagcgg atgtctggcg ctattggtga
     1081 ggagccggcg ggtaaccgca tagccgagcg catgaactcg atgcgggaac agatgcttta
     1141 tggggcaaaa agctccgaat atttttcaa
//
LOCUS       DATCBB010000504         1284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_242863, whole genome shotgun sequence.
ACCESSION   DATCBB010000504 DATCBB010000000
VERSION     DATCBB010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242863"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VII37_05555"
     CDS             complement(<1..352)
                     /locus_tag="VII37_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5069227.1"
                     /translation="MTVKDLTQQESTKQGTAKPESGKQVSTKQVQQGHMGRAFKLEDT
                     RNIGIMAHIDAGKTTSTERILFYTGVTYKIGSVDEGTATMDWMEQERERGITITSAAT
                     TASWVRNDKKYRVNI"
     gene            complement(385..855)
                     /gene="rpsG"
                     /locus_tag="VII37_05560"
     CDS             complement(385..855)
                     /gene="rpsG"
                     /locus_tag="VII37_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY5069228.1"
                     /translation="MPRKGLVTRRAQASDPVYDSQLVDKFICSMMWEGKKSTAQRAFY
                     GAMEQVAKKTNDDALKIFNKAVENVKPLLEVKTRRVGGANYQVPVEVNPFRRQSLAIR
                     WLLQYSRERSGKTMIDKLADELIDASNARGGAMKKKEDVHRMAEANKAFAHYRW"
     gene            complement(935..>1284)
                     /gene="rpsL"
                     /locus_tag="VII37_05565"
     CDS             complement(935..>1284)
                     /gene="rpsL"
                     /locus_tag="VII37_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY5069229.1"
                     /translation="QVQVKTKSPALMGCPEKRGVCIRVYTQTPKKPNSALRKVARVRL
                     TNSIEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTLDAAGVENRKQGRS
                     KYGAKRPKAGQTK"
BASE COUNT          243 a          380 c          318 g          343 t
ORIGIN      
        1 taatgtttac gcgatatttc ttgtcgttgc gcacccagga agcggtagtg gccgcgctgg
       61 taatggtgat gccgcgctcg cgctcctgct ccatccagtc catggtggca gtgccttcat
      121 ccaccgaacc gatcttgtag gtcacaccgg tatagaaaag gatgcgctcc gtcgacgtgg
      181 ttttcccggc gtcgatatgc gccataattc cgatgttgcg cgtgtcttcg agtttgaatg
      241 ccctgcccat gtgtccttgt tgaacctgct tagtagatac ttgtttccca gattccggtt
      301 ttgccgttcc ttgcttcgtc gattcctgtt gcgttaagtc ttttaccgtc acgattcaac
      361 cttcaattca atagcctgac gctgttacca gcgatagtgc gcaaaagcct tgttcgcttc
      421 cgccatgcga tgcacgtctt cctttttctt catggcgccg ccgcgagcgt tcgaagcgtc
      481 aatcaactcg tcagccaatt tgtcgatcat cgtcttccca gagcgttccc gtgaatactg
      541 cagcagccag cgaatcgcga gcgattggcg ccggaaagga tttacttcga caggaacctg
      601 gtagttcgcg ccgccaacgc gccgcgtttt tacttccagc aacggcttca cattttcgac
      661 tgctttatta aaaatcttca gcgcatcgtc gttcgtcttc ttggcgactt gctccatcgc
      721 gccataaaac gcgcgctgcg cagtggattt cttgccttcc cacatcatcg agcagatgaa
      781 cttatccacc agctggctgt cataaaccgg gtcgctggct tgcgcccggc gagtaactag
      841 tcctttgcgt ggcattgcgc tgtatagctc cttcagaaga ttgcaaaaac ccgaaaactt
      901 gcgagtcttg cttacaattt ctatcgattt cgttttactt ggtctgcccg gccttcgggc
      961 gcttagcccc gtatttcgag cggccctgct tgcgattctc tacgcccgcg gcgtccagcg
     1021 tgccgcgcac aatgtggtaa cgcactcccg gcaaatcctt cacgcgcccg ccgcgcacca
     1081 gaacgatcga gtgctcctgc agattgtggc caacaccagg aatgtaagtg gtgacttcaa
     1141 tcgaattcgt cagacgcacg cgcgcaacct tacgcagtgc agagtttggt ttcttgggag
     1201 tttgcgtgta cacgcgaatg caaacgccgc gcttctccgg gcagcccatc aacgcaggag
     1261 acttggtttt aacctggacc tgct
//
LOCUS       DATCBB010000505         1360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_406271, whole genome shotgun sequence.
ACCESSION   DATCBB010000505 DATCBB010000000
VERSION     DATCBB010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406271"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..910)
                     /locus_tag="VII37_05570"
     CDS             complement(266..910)
                     /locus_tag="VII37_05570"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY5069230.1"
                     /translation="MPWSDIYRVIGVAPQTVAHDVRHWIAQLGGVGLIPLGLLDASVV
                     PIPGSMDVLTIVLAARDSKLWFYYAGMATLGSVIGALLTFRLARRGGKETLKRKVSAA
                     RAERIIEIFSRWGFWSIAIAALLPPPVPMVPFVIAAGATQYSTEKFLAALTLGRVIRY
                     SLLALLAARYGRQIINFIVHPGHPSLYVAFGVAVIVSVIAFVMLSARKKKRVHA"
     gene            complement(1021..>1360)
                     /locus_tag="VII37_05575"
     CDS             complement(1021..>1360)
                     /locus_tag="VII37_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069231.1"
                     /translation="EELDEFLVRYNMNGIELNEEGFQKLGEEISLDSIETKLEMPWGK
                     ENVTLFFGEVPMGESVELLVLRKGFARQLLADGKVGEVSSHPYYEVCTSADLYELVAA
                     LIRSQQVAAV"
BASE COUNT          319 a          436 c          288 g          317 t
ORIGIN      
        1 ttcgcctttt tcgggaattc gaccgtgatg tgcgcttatt ttccccctgc atgcagcaga
       61 tttctgccgt cggtttatat tttcgcaatt acagtatcag tactttggct ctagtacgaa
      121 atatggtccg cataggtccg tggaatttgt tcccgcgcaa ctaggtagtt ttcgttctta
      181 ttgttgatcg ccgcatttca cggctcgagg ctaactcgga cttgatccgc ggaatttcct
      241 catttgcgag acacactttg caacactaag cgtgcacgcg tttctttttc cgcgccgata
      301 acatcacaaa cgcaatcacg ctgactatga ccgctactcc gaaagccaca tacagcgacg
      361 gatgccccgg atgcacgatg aaatttatga tctgccggcc gtaacgcgcc gcgagcaagg
      421 ccagcaagct gtaccgaatc acccgcccca acgtcagcgc cgcgaggaat ttctccgtgg
      481 aatactgcgt ggctcccgcg gcaatcacaa acggcaccat gggcactggc gggggcaata
      541 acgccgctat ggcaatcgac cagaaccccc agcgcgagaa aatttctatg attcgttcgg
      601 cacgcgccgc agaaactttt ctttttaatg tttctttgcc gcctctgcgt gcaagccgaa
      661 aggtcaacag cgccccgatt accgacccta gggtcgccat ccccgcataa taaaaccaca
      721 gcttcgagtc tcgcgcagcc agcacgatcg tcagcacatc catgctgccc ggaatcggca
      781 ctaccgaagc gtccagcaat cccagcggta tcaatcccac gccacccagt tgcgcaatcc
      841 aatgtcgcac atcgtgcgca accgtttgcg gcgcgacacc aataacccgg taaatgtcgc
      901 tccaaggcat ggcacattct gccacgccat tgacagcttt ctaaaatgtt ttgcggtatt
      961 cgctacttaa ggaaacaact cacttcgaac gcaatttgcg gcccatgcag tccccatcta
     1021 tcaaaccgct gccacctgct ggctgcgtat caatgcagca accagttcat acaaatctgc
     1081 gctggtacac acctcgtaat aaggatgcga cgaaacctca cccacttttc catccgccaa
     1141 taactgccgc gcgaatccct tgcgcagcac cagcagttcc accgactcgc ccatcggcac
     1201 ttctccaaaa aacagcgtca cattctcttt tccccacggc atctccagct tggtctcgat
     1261 cgaatccaga gatatctcct cgcccagttt ctgaaaccct tcttcgttca attcgatgcc
     1321 attcatgttg tagcgcacca gaaattcatc caactcctcg
//
LOCUS       DATCBB010000506         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_308706, whole genome shotgun sequence.
ACCESSION   DATCBB010000506 DATCBB010000000
VERSION     DATCBB010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308706"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2401)
                     /locus_tag="VII37_05580"
     CDS             complement(44..2401)
                     /locus_tag="VII37_05580"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY5069232.1"
                     /translation="MDEQNRLQLYEPRQSALERVNHSASAYPVEDREAVPDLLEYWRV
                     IQKRYATALVVLLVVFMIGLFATFRGKPVYEARALIEIQKENPDVPTLQELFQIEGVS
                     DAYIETQNRILKSENLARRVTTQLGLEKLPEFTRGSGSWQIAREKPAPQPAQPRFGVS
                     TAADKNVPEEVLKNFEERLTVEPVKRSRLIEVTFESNDPNIAAQVVNTLTSAYIDANL
                     EARWQAAQKASDWLSQQLLGMKAKLEKSEDELQKYGRGNGLLFLETEKGTSENIVVQR
                     LRELQQELTKAQADRYAKESLYRLLEERNYAELPGVFDNKLIQELTARLADLQREQSR
                     LSANFNPSYPRVKELQSQIDESKAMLDAERARAAAGIANDYKAAVSHERMLQQAFVEQ
                     ERQANDIAGKSVQYNILKREADTNKQLYVGLLEKLKETGVSSSLKATNIRVVDPAYPP
                     KKFARPRILLDLSITLIVGMCLGIAAAFLQEHLDNTLKSSEDIERFLQIPSLGAVPAM
                     ELSANPRRMHGFQSRTPMLAAEKANGTNGHNGRNGNNGSKNGAPLAPPWNRIEVQDGG
                     GHHNGALAEAFHGLRTSVLLSTAKRPPATLLVTSAQQGEGKTTVAANLAASLAQLGDS
                     VLLIDADLRRPSLQKFFQVSRSTGLVNYLTGDSDWRSLVWQAAPIGVSVLFCGPVPPN
                     PADLLSSEYMRTLIREASKEYKFVVLDSPPLLNLSDSRILATLVDGVILVVGGGSTPR
                     ELVQRAYLSAVDAGSHVIGATINFADVRNDYYYSGYHQEAEESEK"
     gene            complement(2406..>3451)
                     /locus_tag="VII37_05585"
     CDS             complement(2406..>3451)
                     /locus_tag="VII37_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY5069233.1"
                     /translation="QFAETRERQTGDYRIGPQDLLDINIFEAPELNRTVRVSENGEVS
                     LPLLGGIHVVRLTARELENTLAAKLREFLKDPHVSVMVTAIESHPVSVIGEVNKPGVF
                     QVRGSKTLLEMLSMAQGLAPEAGDEVLVMRNAGYNRGQEGGAQTTPAGSTGTGRKDED
                     GIKSGSGSDSNGNGDGALKKNATTSVEETNNAGAKQEREQGGDTIAINLRHLLNSRDP
                     RFNVPIYAGDIVKVTRAGIVYVVGAVKKPGGFTVKGNEQMSLLKAIALAEGLSSTSSK
                     GHTRIIRTDEGSGKRSEIPVPLGKILDGKAPDLNLQAADIVFVPSSTGKTVLFRSTDA
                     VVNTATGLAIFHP"
BASE COUNT          667 a         1124 c          794 g          866 t
ORIGIN      
        1 ttcagcactc gcccactctc ccgccccgaa aaaccgcgct tctctacttt tcggattcct
       61 ctgcttcctg gtgatatccc gaatagtagt aatcgtttct cacgtcggca aaattaatcg
      121 tcgctccaat cacgtgcgag cccgcgtcca ccgccgacaa atacgctcgt tgtaccaatt
      181 cgcgcggtgt gctgccgcct cccacaacca ggatcacgcc atccacgagc gtcgccagga
      241 tgcggctgtc tgaaagattc agcagcggcg gcgaatccag caccacaaat ttatattcct
      301 tcgacgcttc gcgaattaac gtgcgcatat actccgaaga aagcaggtca gcggggtttg
      361 gcggcaccgg cccgcagaaa agcacggaaa cgccgatggg cgcggcttgc cataccagac
      421 ttcgccagtc gctatctccg gtgaggtaat tcaccagtcc agtcgatctc gagacttgaa
      481 aaaatttctg taagctcggc ctgcgtaggt cggcgtcaat caacaaaacc gaatcgccca
      541 gttgggccag cgacgccgcc aggttcgccg ccaccgtagt tttaccttcg ccctgctgcg
      601 cgctagtcac cagcagcgtc gctggcggac gctttgccgt cgagagcagc accgaggttc
      661 gcagtccgtg aaatgcttcg gccagcgctc cattgtgatg accgccgccg tcctgcactt
      721 cgatgcggtt ccatggcggc gccaatggcg cgccgttctt cgatccgtta tttccattgc
      781 gaccgttatg tccgtttgtt ccgttggctt tttccgccgc aagcatcggc gttctactct
      841 ggaacccatg cattcgccgt ggattggcgg aaagctccat ggccggcacg gcgccgagcg
      901 acggtatttg caaaaatcgc tcaatatcct cggagctttt caacgtgtta tccagatgct
      961 cctgcaaaaa cgccgctgca atccccaggc acatgcccac aatcaacgtg atcgataaat
     1021 ccagcaggat gcgcggccgg gcaaattttt tcggcgggta agccggatca accacacgaa
     1081 tattggtcgc cttcaaactg ctcgacactc ccgtctcttt caatttttcc agcaggccca
     1141 catacaattg cttgttggtg tctgcttcgc gcttcaaaat attgtattgc accgattttc
     1201 cagcgatatc gttggcttgc cgctcctgct ccacaaatgc ttgttgcagc atccgctcat
     1261 ggctcaccgc cgctttgtaa tcgttagcaa tgccggcggc cgctcgcgcg cgctccgcgt
     1321 ccagcatcgc cttgctctcg tcgatctggc tctgcagttc cttgacccgc gggtagcttg
     1381 gattaaaatt cgcgcttagt cgcgactgct cccgctgcaa atccgccagc ctggcggtca
     1441 attcctggat cagcttgtta tcgaaaactc ccggcaactc cgcgtaattc ctttcttcca
     1501 gcaaccggta taaagattct ttcgcgtacc ggtccgcctg agccttggtc aattcctgtt
     1561 gcagctctcg caaccgctgc actacgatat tttcgctggt acccttttcc gtctccagaa
     1621 aaagcaatcc gtttccccgg ccatatttct gcaactcgtc ttctgatttt tcgagcttgg
     1681 ccttcattcc cagcagctgc tgcgatagcc agtcggaagc tttctgcgcc gcttgccagc
     1741 gcgcttccag atttgcatcg atgtatgcgg acgtcagcgt gttcactacc tgcgcggcaa
     1801 tgttcggatc gttcgattcg aacgtcactt caatcagccg gctccgcttc acaggttcga
     1861 ctgttagtcg ctcttcaaaa tttttcagca cttcttctgg aacgttcttg tcggcggccg
     1921 tgctcacccc aaaccgcggc tgtgccggct gcggcgccgg cttctcccgc gcaatttgcc
     1981 aacttccgct accccgcgta aactcgggca acttctcgag ccccaattgc gtagtcactc
     2041 gccgcgccag attttcgctt ttcaaaatac ggttttgcgt ttcaatgtac gcatcggaaa
     2101 cgccctcaat ctgaaacaac tcctgcagcg tcggcacatc gggattttct ttctgaattt
     2161 cgatcagcgc tcgcgcctca tagaccggtt tgccgcgaaa ggtcgcaaat agcccaatca
     2221 taaaaactac cagcaacacc accagggccg ttgcgtaccg cttctgaatt actcgccaat
     2281 attccagcaa atccggtacc gcctcgcggt cttccaccgg ataagcgctc gcgctatgat
     2341 tcacgcgttc cagcgcactt tgtcgcggtt cgtaaagttg caatctgttt tgttcgtcca
     2401 tatttttatg ggtggaaaat cgccaacccg gtggcggtat tgaccacggc atccgtgctt
     2461 ctaaaaagta ctgtcttccc cgtgctgctg ggcacaaaca caatatccgc ggcctgcaaa
     2521 tttaggtccg gagcttttcc atctaaaatc tttccaagtg gtaccggaat ttccgagcgc
     2581 ttgccgctgc cctcatcggt ccggataatg cgcgtgtgcc ccttcgacga ggtgctcgat
     2641 aatccttccg ccagcgcaat cgccttcagc agcgacattt gctcgttccc tttcactgtg
     2701 aaaccgccgg gctttttcac cgcacccacc acgtatacaa ttcccgcccg cgtgacttta
     2761 acaatgtcgc ccgcatagat cggcacgtta aatcgcgggt cgcgcgaatt cagcaggtgc
     2821 cgcagattga tcgcgatcgt gtcaccgcct tgctcccttt cttgtttggc acccgcgttg
     2881 tttgtttctt cgaccgacgt tgtggcattt tttttcagcg cgccgtcgcc attgccattg
     2941 ctatccgatc ccgacccact tttgattcca tcttcatcct tgcggcccgt ccccgtactt
     3001 cccgctggcg ttgtctgcgc gccaccttct tggccgcggt tatatcccgc attcctcatc
     3061 accaacactt cgtcgcccgc ctcaggtgcc aacccttgcg ccatggaaag catctccagc
     3121 agcgtcttcg accctcgcac ctgaaaaact cccggcttat tcacctctcc aatcaccgaa
     3181 accggatgac tctcaatggc ggtgaccatc acgctcacgt gcggatcctt caaaaactcc
     3241 cgcaacttcg ccgctagcgt attctccagc tcccgcgccg tcaatcgcac tacgtgaatg
     3301 ccccccagca gcggcagcga cacttcgcca ttttctgaaa cccgtacggt acgattcagt
     3361 tccggagcct caaaaatatt tatatccaat aaatcctgcg gcccgatgcg gtaatctccc
     3421 gtttgccgct cgcgcgtttc tgcaaattgt t
//
LOCUS       DATCBB010000507         1044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_406290, whole genome shotgun sequence.
ACCESSION   DATCBB010000507 DATCBB010000000
VERSION     DATCBB010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406290"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VII37_05590"
     CDS             <1..189
                     /locus_tag="VII37_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5069234.1"
                     /translation="LDEVRKVHIQRVLAMCMGNRLRAAQVLGIGRTSLYRYLKRDQLE
                     HSLQVKLRGAAAAGSVRT"
     gene            516..>1044
                     /locus_tag="VII37_05595"
     CDS             516..>1044
                     /locus_tag="VII37_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5069235.1"
                     /translation="MSKRLAGIGVLVLALLSANIARAQELTPADKERAMQYLENTKKN
                     IVDATKGLSAAQWNFKAGPDRWSVAEVMEHIAAAEDLIRGMIIEKAMKGGPPEPGRDL
                     KKIDDGVVAMIPDRSHKAQAPDALKPTNRFASPEGSLKHFLESRAQTEEFLNNTPDLR
                     QHVTDSPLGMKLDAYE"
BASE COUNT          248 a          281 c          296 g          219 t
ORIGIN      
        1 ctagacgaag tgcgcaaagt gcatattcag cgggtgctgg cgatgtgcat gggaaaccgc
       61 ttgcgggccg cgcaagtgtt aggcatcggt cgcaccagcc tctatcgtta cctgaaacga
      121 gatcagctcg agcacagtct gcaggtaaag ctgcgcggcg cggccgccgc aggatctgtg
      181 cgaacttgaa ttttagcggt ggcgaagcta gagctcgata gtttcgatct ctcgaagcat
      241 tattgttcta gctgctggct acaacatcgc tcgcccgcct acaagcattt ctgctaaatc
      301 gcaaatacct tccatcatcg cggcgctttc aaacatactg gccttacctc gcgggccttt
      361 cgcgtcatcg cggggcgtag accgtccttg caatcgctga tacagtgtgt gcgcccggaa
      421 cgtctcaggc cgcgtgagcg atcagcggga tcctcttacg cgcagatcga cgacttgtct
      481 ggtcattcta aatttttgaa ggagagaaga attttatgag caaacgcttg gcgggaattg
      541 gtgtccttgt tttggcattg ctcagtgcca acatcgcgcg ggcccaggaa ttaactccgg
      601 cagacaaaga gcgcgccatg cagtatctcg aaaacaccaa gaaaaatatc gtggacgcca
      661 cgaaagggct gtcggcagcg cagtggaatt tcaaagccgg gccggaccgc tggtcggtcg
      721 ccgaggttat ggaacatatc gcggcggcgg aagacttgat acgtggaatg attatcgaaa
      781 aggccatgaa gggtggccca ccggaaccag gccgcgacct caagaagatc gatgatgggg
      841 tggtagccat gattccggac cgctcccaca aggcacaagc tccggacgcg ctaaaaccca
      901 ccaatcggtt tgcttcgccg gaaggttcgt taaaacattt tctcgaaagc cgcgcgcaga
      961 cggaggaatt tcttaataac accccggact tacgccagca cgttacagac agcccccttg
     1021 gtatgaagct ggatgcctac gagt
//
LOCUS       DATCBB010000508         1217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_406309, whole genome shotgun sequence.
ACCESSION   DATCBB010000508 DATCBB010000000
VERSION     DATCBB010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406309"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VII37_05600"
     CDS             complement(<1..232)
                     /locus_tag="VII37_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069236.1"
                     /translation="MDYSTGTDLTASSEAFRPGPAAAPWWRSLRLAVSFPAMMAVLLM
                     ALALIGAEDRLLDPDTWWHITVGQEILKTHTWP"
     gene            complement(233..1078)
                     /locus_tag="VII37_05605"
     CDS             complement(233..1078)
                     /locus_tag="VII37_05605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5069237.1"
                     /translation="MGKNVTRQSTDVAVSALHDREAAFHDAWASETELSEILVRESFE
                     APTAMENQFILQCMGDLKGKRLLDIGAGLGESSVYFALQGAEVTIVDVSPRMVNTALD
                     LGARFGVKLQGIVSGAEILEVSPASYDLIYIANTIHHVQNRPALFAQMQRALKPGGRF
                     FSYDPLAYNPAINLYRRMASAVRTPDESPLSTADLDSARKYFRHVGHREFWVSSLLLF
                     VKYFLIDRVHPNEDRYWKRILRETPLSLRWWMPLRTLDKFLTRIPLVRWLAWNMVMWG
                     EKAAD"
BASE COUNT          290 a          353 c          309 g          265 t
ORIGIN      
        1 ttggccacgt gtgagtttta agaatttcct gccctacggt gatgtgccac cacgtgtcgg
       61 gatcaagcag ccggtcttcc gcgccgatga gcgctaaggc catgagcagc accgccatca
      121 tcgcgggaaa agaaaccgcc agccgtaggg agcgccacca cggcgccgct gctggccctg
      181 gccggaacgc ttcgctgctg gccgtcaagt ccgtaccggt ggagtagtcc atttaatcgg
      241 cggctttctc accccacatc accatgttcc acgccaacca gcgaaccagc ggtatgcgtg
      301 taagaaattt gtcgagtgtt cgcagtggca tccaccaacg caaactgagt ggtgtttcac
      361 gaagaattct tttccagtaa cggtcctcat tgggatgcac gcgatcgata aggaaatact
      421 tcacaaaaag cagcaaggag gaaacccaga attcccggtg tcccacgtgc cgaaaatatt
      481 tccgggctga atctaagtcc gctgtggaaa gtggcgattc gtccggcgtt cgcactgcgc
      541 tggccatgcg ccggtaaaga ttgatagcgg gattgtaggc taggggatca taagaaaaaa
      601 atcttccgcc gggtttcagc gcgcgctgca tttgagcgaa aagcgcaggg cggttctgga
      661 cgtggtgaat ggtattggca atgtaaatca aatcataaga cgccgggctc acttccagga
      721 tttccgcccc cgacacgatt ccctgcagct tgacgccaaa tcgcgcgccg agatcgagcg
      781 ccgtgttcac catgcgcgga gaaacatcca cgatagtaac ctccgcgcct tgtagggcaa
      841 agtacaccga ggactccccg agtcccgctc caatatccag caacctcttt cctttcaaat
      901 cacccatgca ctgaagaatg aactgattct ccatagccgt cggcgcttca aaactttctc
      961 gaacaagaat ctcacttaac tccgtttcgc tagcccaggc atcgtgaaaa gcggcttcgc
     1021 gatcatgcag cgcgctcact gctacatccg tggattgtcg cgtaacattt tttcccaaga
     1081 gatcgtccct agtgtttccc tgcgataaac agcgaagacg ggttggtcac gcgcttatca
     1141 cctttgaatc ccaacatctc cctaaccaca taaagcggcc gatgtttgga ttcgccgtaa
     1201 atgcgcccga tgtattc
//
LOCUS       DATCBB010000509         1239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_471013, whole genome shotgun sequence.
ACCESSION   DATCBB010000509 DATCBB010000000
VERSION     DATCBB010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471013"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1082
                     /locus_tag="VII37_05610"
     CDS             39..1082
                     /locus_tag="VII37_05610"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5069238.1"
                     /translation="MTKIAILGGGSWGTALSIVLCRSHNSHELSLWMRDSALAYAVNN
                     SRVNELYLPACVIPPAVNITSDLASALAGADVIVGAMPSAYARIVYSRALPLVEKTAR
                     VFVSATKGLEPSTHSRITEVLSQLLSEKFSPVPHIAVLSGPSFALEAARGDPTAVVVA
                     SQSQQPVENTRTAERVADYIQAEFAGPSFRLYTNDDVVGVELAGAMKNVMAIAAGACQ
                     GLGLGTNAIAALITRGLAEMARLALALGARAETLSGLAGLGDLVLTCTGSLSRNRQLG
                     IELGKGRALPEIVASMRMVAEGVGTAGALLGLASEYQIELPITTQVNAILHRGKPPRE
                     AMEEIMGRPQKRE"
BASE COUNT          251 a          386 c          339 g          263 t
ORIGIN      
        1 gaaccgaaat tttccgccag taagagcgcc aaaaaagaat gaccaaaatc gccatccttg
       61 gcggtggaag ctggggcacc gcgttatcca tcgtcttgtg ccgtagccac aactcccacg
      121 aactttcact atggatgcgc gactcggcct tggcgtacgc agtcaacaac agtcgcgtga
      181 atgagcttta cctgccggcg tgcgtgatcc ctcctgccgt caacatcact agcgatctcg
      241 cttctgcact ggctggagcc gacgtcattg taggagccat gccttccgcg tatgcgcgga
      301 ttgtgtattc ccgagctttg ccacttgttg agaaaactgc acgcgtcttc gttagcgcca
      361 caaaaggcct ggagccctca acgcactcgc gaatcaccga agtgctgtcg caactattgt
      421 cagagaagtt ttcgccggtc ccgcacatcg cggtgctttc tggaccctct tttgccctgg
      481 aagccgctcg gggagaccca accgctgtgg tggtggcgtc tcagagtcag cagcctgtcg
      541 aaaacacccg tactgccgaa cgtgtcgcag attacattca ggccgagttc gcgggcccat
      601 ccttccgcct gtataccaac gacgatgtag tcggtgtcga gttggcgggc gcaatgaaaa
      661 acgtgatggc catcgccgct ggtgcctgcc agggtttggg cctgggcacc aatgccatcg
      721 cggctttgat cacccgtggc ctcgcggaaa tggcacgtct cgccctggcg ctaggtgcgc
      781 gcgctgaaac cctgagcggg ctggccgggc tgggcgatct ggtgctgacc tgcaccggct
      841 ctttgagccg caatcgccaa ctgggaattg aactcggcaa aggacgcgct ctgcccgaga
      901 tagtcgccag catgcgaatg gtcgccgaag gtgtcggaac cgcaggtgcc ctcttaggat
      961 tggccagcga gtaccaaatt gaactcccca tcaccacgca ggtaaatgcc atcctgcacc
     1021 gcggcaaacc cccgcgagag gccatggagg aaatcatggg ccgccctcag aaacgcgagt
     1081 agcccgtaga cgctcgccac cctcctgtca cttagtgata gccatttgac gtaccaatat
     1141 agtaatatac gtaatgccct tcgcgtcgcc gacgttcatc gctactcgcc gcatatccgc
     1201 cgtgaccttt tccagtccgt ttcccgtcta ttttcagtt
//
LOCUS       DATCBB010000510         1269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_373412, whole genome shotgun sequence.
ACCESSION   DATCBB010000510 DATCBB010000000
VERSION     DATCBB010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373412"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VII37_05615"
     CDS             <1..180
                     /locus_tag="VII37_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069239.1"
                     /translation="DEEARSLAASYGAAALLDKMSLYTELLPAIMRLGEPSANPPFKL
                     DPKAGREAAPLGLSE"
     gene            264..>1269
                     /locus_tag="VII37_05620"
     CDS             264..>1269
                     /locus_tag="VII37_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY5069240.1"
                     /translation="MKIALAQFNPTVGDFAGNAARILSLATTAHQRGADLAVFSELCL
                     CGYLPQDLLERPAFIDRNHKELKELAAKLPLPSIVGYAGRVKNGREKSIGKSIANKAA
                     LICEGRIVFEQSKMLLPTYDVFDESRYFQPADRQFTFGLGNEQLGITICEDVWNDKNF
                     WANRRYDRDPVTEIIAQGTTVLLNVSASPYTIDKRGLRLEMLRSIAVHHRRPIVYVNQ
                     VGGDDSLIFDGASMALTADGKVAAQARAFEEDLVFFDTVTGKGEIHDQPHEEIAYAYQ
                     AIVTGTVDYVQKCGFKKVLVGLSGGIDSAVVAAIAADALGPENVLGVSMPGPYSSQGS
                     K"
BASE COUNT          286 a          358 c          333 g          292 t
ORIGIN      
        1 gatgaagaag ctagatccct ggcagcgagt tacggtgccg ctgcgctcct cgataagatg
       61 agcctgtata ccgagctgct gccggctatt atgcgattgg gggagccctc cgcaaatccg
      121 ccctttaaac ttgatcccaa agcgggcagg gaagcagcgc ccttgggact ttccgaataa
      181 ctgctcctcc tctctcactc cgcatcgccg caccccgcac catttgcttt caagcgcaac
      241 gaaccgtatc ctgaaatccg ggcatgaaaa tagcgctcgc gcagtttaat cccaccgtcg
      301 gtgattttgc agggaatgcc gctcgtattt tgagcctggc caccaccgcc catcaacgag
      361 gcgcggatct agccgttttt tccgagcttt gtctctgtgg ctatttgcct caggacctgc
      421 tggaacgccc tgcttttatc gatcgcaatc acaaggaatt aaaagagctc gccgccaagc
      481 ttcccttgcc gtccatcgtg gggtatgccg ggcgcgtcaa aaacggccgc gagaaatcta
      541 tcgggaaatc catcgccaac aaagccgcgc ttatctgcga aggccgtatt gtttttgaac
      601 aaagcaaaat gcttctgccc acctacgatg tcttcgatga atcgcgatat tttcagccgg
      661 ccgacaggca attcaccttt ggacttggaa acgagcagct gggcattacc atttgcgaag
      721 acgtgtggaa cgataaaaat ttctgggcga atcgccgtta tgatcgagat ccggtaacgg
      781 agattatcgc gcagggaact accgtccttc tgaatgtttc cgcttcgccc tacaccatcg
      841 acaaacgagg cttgcgactt gagatgttgc gctctatcgc tgtgcatcat cgccgcccta
      901 tcgtgtacgt gaaccaggtt ggaggcgacg acagcttgat ctttgatggc gccagcatgg
      961 cgctcactgc cgatggcaaa gttgctgcac aagcgcgtgc cttcgaagaa gacctggttt
     1021 tcttcgatac tgttaccggc aaaggggaaa ttcacgatca gccgcatgaa gaaatcgcct
     1081 acgcctatca agccattgtc accggcacag tagactacgt gcaaaaatgt ggtttcaaaa
     1141 aagttctcgt gggtttgagc ggcggtatcg attccgcggt cgtcgcggcc atcgctgcgg
     1201 atgcgctggg accggaaaat gtactcggcg tctccatgcc cggcccgtat tcgtcgcagg
     1261 gaagtaaga
//
LOCUS       DATCBB010000511         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_179200, whole genome shotgun sequence.
ACCESSION   DATCBB010000511 DATCBB010000000
VERSION     DATCBB010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179200"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VII37_05625"
     CDS             <1..920
                     /locus_tag="VII37_05625"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY5069241.1"
                     /translation="PPPSSGGVAVIEALNMLKQTFLKGWDDPQSVHMVVEVMRRVFAD
                     RAAYLADPDYSNVPVAGLISDCYAKERAASIDPQHASSSKQVKAGNPQACGISAGNAD
                     APNTVVSLGEGPHTTHFSVVDAAANAVASTYTLNDSFGSHATSSAGFLLNDEMDDFTT
                     QPGVPNALFGLIQSDANAIAPNHRPLSSMTPTMLLRDGKLSFVTGSPGGPTIISATLL
                     SVINWMRLGMDAQAAINAPRFHHQWLPDELVMEKEFSPVFEQALNANGYATKRKGHIG
                     LVNAIGIDAQTGDRLGAADPRDHGSAVGY"
     gene            1050..1913
                     /locus_tag="VII37_05630"
     CDS             1050..1913
                     /locus_tag="VII37_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5069242.1"
                     /translation="MHRLLSSYLFISRKVTPELLGQIANAGFEGVELFCSRSHFDYTS
                     RAQIQAMAEALSTHKLSLESLHAPNSRDLSAAREGGTPLSICEVERVRRIEAMDELKR
                     AIDVAEDLPYSRMVLHMGSSRETADPRKRDAAFSTLEHLVLHAHHCGVTLCIENTTSE
                     MGDPSYLRTFVDETRLSGLRFNFDIGHAHLCDGPAEERIEKCFEPMRDLVASVHLHDN
                     HGEKDEHLPPYAGNIDWPAAIKLLKTTPEGEKLPIVLELKEKTGNDAPAATELLESAR
                     AAMDKFEEEWG"
     gene            2054..>2844
                     /locus_tag="VII37_05635"
     CDS             2054..>2844
                     /locus_tag="VII37_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY5069243.1"
                     /translation="MTIISIEDAGKYEGQEVTIRGWLYNLRESGKLMFPIFRDGTGII
                     QGVCALKENPEAFEALKGLTQESSVIVTGKIRAEQRAPGGFEMGVSKIQVLQKVSEAQ
                     PYPIQLKEHGVDFLLDHRHLWIRTPRQASILRIRAEAIRAARNFMDSQGYILTDAPMF
                     TPAACEGTSTLFEVNYIDDQKAYLTQSGQLYIEATAMALGKVYTFGPTFRAEKSKTRR
                     HLTEFWMLEPEAAYAELGDMMNLAEGLVSAVVQSVLKNKSRELEML"
BASE COUNT          653 a          849 c          732 g          610 t
ORIGIN      
        1 ctccgccgcc cagctccggc ggagtcgccg tcatcgaagc actcaatatg ctcaagcaaa
       61 ctttcctgaa aggctgggac gatccgcaaa gcgtgcacat ggtagtggaa gtgatgcgtc
      121 gcgtcttcgc cgatcgcgcc gcctatctcg ccgatcccga ctattcgaat gtacctgtcg
      181 ccggactcat cagcgattgt tacgccaaag agcgcgccgc ctccatcgat ccgcagcacg
      241 cctcgtccag caaacaggtg aaagccggca atccgcaggc ctgcggaatt tcagctggca
      301 acgcagacgc cccgaacacc gtcgtcagcc ttggcgaagg cccgcacacc acgcactttt
      361 ccgtggtcga cgccgccgcc aacgccgtcg ccagcaccta cacgctcaac gacagcttcg
      421 gttcgcacgc caccagctcc gccggatttt tgctcaatga cgaaatggac gacttcacca
      481 cgcaaccggg cgtgcccaac gcgcttttcg gcttgatcca atctgacgcc aacgccattg
      541 cgcccaatca ccgcccgctc agctccatga ctccgaccat gctgttgcgc gacggaaaat
      601 tgagttttgt caccggatct cccggaggcc ccaccataat ttccgccacg ctcttgagcg
      661 tcatcaactg gatgcgtctg ggcatggacg ctcaagccgc catcaacgcg cctcgttttc
      721 atcatcaatg gctgcccgat gaacttgtga tggaaaagga attttcgccg gtatttgaac
      781 aggctcttaa cgccaatggt tacgctacaa aacgtaaggg tcacatcggg ctggtaaatg
      841 ccattggcat cgacgcgcag acgggtgacc gtctcggtgc cgccgatccc cgtgatcacg
      901 gctccgccgt gggctactga ccttgggtcc actgaccatc accccgaata taagtattcg
      961 cgcaactact cttgttattc tcgcgtcaac gtcgccaact cttcagcgcg tcaggcattt
     1021 tctaggaata taattcttaa gatcagctca tgcacagact tctctcctct tacctgttta
     1081 tttctcgcaa agtgactcct gaacttctgg gacagatcgc taacgccggc tttgagggcg
     1141 tggaactttt ttgttcacgc agccattttg attacacctc acgcgcccag atccaggcca
     1201 tggctgaggc tttgagtacg cacaaactgt cgcttgaatc gctgcacgcg ccgaatagcc
     1261 gcgatctcag tgcggcgcgt gaaggcggca cgccgctctc catctgcgaa gtggaacgcg
     1321 tacgccgcat cgaagccatg gatgaactga aacgcgccat cgatgtggct gaagatttgc
     1381 catattcgcg catggtcctg cacatgggaa gttcacggga aaccgcggac ccgcggaaac
     1441 gcgatgctgc gttcagcacg cttgaacatc tcgtgcttca cgctcaccat tgcggcgtga
     1501 ccttgtgcat cgaaaatacg actagcgaaa tgggcgatcc gtcttacctg cgcacctttg
     1561 tggacgagac ccggctgtcc ggcttgcgct ttaattttga catcggccac gcgcatcttt
     1621 gcgatgggcc agcggaagaa cgcatcgaaa agtgtttcga gcccatgcgc gatctcgtcg
     1681 ccagcgtaca cctgcatgac aatcacggtg aaaaagacga acatcttccg ccctatgccg
     1741 gcaatatcga ctggccggca gcgatcaaat tactcaagac cacccccgag ggcgagaaac
     1801 tccccatcgt gctcgaatta aaagagaaaa ccggcaacga cgcgcccgcc gccactgaat
     1861 tgctggaatc cgcgcgagcc gcaatggaca aatttgaaga agaatggggc tagccctcca
     1921 accctctcga cttttcaact tttcaacccg ccactgctaa aatacttatg agcaagcgat
     1981 ctagtggttt ggccctgtca gcattcctgc ttggatcggc cgcgaagatt tgcgaactca
     2041 ggaaaattaa cgaatgacta ttatttccat cgaagacgcc ggcaagtacg aggggcagga
     2101 agtaaccata aggggctggc tctacaattt acgtgagtcg ggcaagctca tgtttccgat
     2161 tttccgcgat ggtaccggga ttatccaggg cgtgtgcgcg ctcaaagaaa atcctgaagc
     2221 gtttgaagct ttgaaaggcc tcacacagga atccagcgtt attgtgactg ggaaaattcg
     2281 ggccgagcag cgtgctccgg gcggatttga gatgggcgtc tcaaaaattc aggtgctcca
     2341 gaaagtttcg gaagcgcagc cgtatcccat tcaactcaag gaacatggcg ttgattttct
     2401 tctcgatcat cgccacctgt ggattcgtac tccgcggcaa gccagcattt tgcggattcg
     2461 cgcggaagcc attcgcgccg cgcgaaattt catggatagc cagggttaca ttctcaccga
     2521 cgctccgatg tttacgccgg cggcctgcga aggcacctcc acgctgtttg aagtgaatta
     2581 cattgacgat cagaaagcgt atctaacgca gagcggccag ctttacatcg aagccaccgc
     2641 tatggcgctt ggcaaggtgt ataccttcgg ccctacattt cgcgcggaaa aatcgaagac
     2701 tcgacgccac cttacggagt tctggatgct cgagccggag gctgcctacg ctgaactcgg
     2761 cgacatgatg aacctcgccg aaggcttagt gagcgctgtg gtgcaatctg tgcttaagaa
     2821 caaatcacgt gaactagaaa tgct
//
LOCUS       DATCBB010000512         1265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_308942, whole genome shotgun sequence.
ACCESSION   DATCBB010000512 DATCBB010000000
VERSION     DATCBB010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308942"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VII37_05640"
     CDS             complement(<1..461)
                     /locus_tag="VII37_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5069244.1"
                     /translation="MTSNPPEATIMPPVPLEDPGGQMTFFEHLVELRKRIITSLIAVA
                     IGAGIGWFVAPYFVSWITKPMLKALSDQHLTQQLVYTNPTGYLSLLISLSIYLGIVLA
                     SPVVLYQFWLFVAPALYKHERSATTGFLFSTVILFLAGIGFGYFMMLPKVL"
     gene            complement(442..1155)
                     /gene="tatA"
                     /locus_tag="VII37_05645"
     CDS             complement(442..1155)
                     /gene="tatA"
                     /locus_tag="VII37_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY5069245.1"
                     /translation="MTAFDTVLYDANSRGNGYLEPMLSIPHMLVVFVIVLVVFGPQKL
                     PELARSLGKLMAEFRKASADFRGAFEQEMKDMERQVREVERKKAADAAAKSADEAPVQ
                     AATFATPAGVETPVAEANNVWVGGGAVSETSTGAEADAFGEPIDARVTEGSTDLVVTP
                     VTESVARTSTGLAETATERANGVATEAESPDKISNGSGSKTAAGTYAGTVAGTTPDTS
                     AETTGPVVSIDLPHDQQPA"
BASE COUNT          289 a          417 c          289 g          270 t
ORIGIN      
        1 ttcagcacct tcggcagcat catgaaatac ccaaaaccaa tgcccgccaa aaacaaaata
       61 accgtagaaa acagaaatcc cgtcgtagcg ctgcgttcgt gcttgtacaa agccggcgcc
      121 acaaacagcc agaattgata cagcaccacc ggcgacgcca acacgattcc cagataaatg
      181 gaaagactga tcaacaggct caggtacccg gtgggattcg tgtacaccaa ctgctgcgtc
      241 aggtgctggt ccgacagcgc cttcaacatc ggcttggtaa tccacgaaac gaaatacggc
      301 gctacaaacc atccgattcc tgcgccaatc gccactgcaa taagtgacgt aatgatccgc
      361 ttgcgcagtt ccaccaaatg ctcaaaaaat gtcatttgcc cgccgggatc ttccagcggc
      421 actggcggca taattgttgc ttcaggcggg ttgctggtca tgaggtagat caatactgac
      481 tactgggccc gttgtttcgg cgctcgtatc cggagtggta ccggcaaccg ttcctgcgta
      541 tgttccagcg gctgtttttg aaccgcttcc attagaaatt ttgtcaggac tttcggcttc
      601 agttgccact ccgttagctc tttccgtcgc cgtctccgcc aatcccgtcg aagttcgtgc
      661 cacagattcc gtcaccggcg taaccaccaa gtcggtcgac ccctcggtca cccgcgcatc
      721 aatcggctct ccaaatgcat ccgcctccgc gcccgtcgac gtttcactca ccgcgccgcc
      781 gcccacccaa acattattcg cttccgcaac cggcgtctcc actcccgcag gcgtagcaaa
      841 cgtcgccgcc tgcaccggcg cctcatccgc agacttagcc gccgcatccg cggccttctt
      901 gcgctccact tcgcgcacct gccgctccat atccttcatc tcttgctcaa atgccccgcg
      961 aaaatccgct gacgccttgc gaaattccgc catcaatttc cccaggcttc gcgcaagctc
     1021 cggcagcttc tgcggcccaa agaccaccag cacaatcaca aacaccacca gcatgtgggg
     1081 aatacttagc atgggctcca ggtagccatt tccgcggctg ttggcatcat agagaacggt
     1141 gtcaaacgct gtcaaactcg cacgcggcgc gtccaaccta tagaatacgc tgtgttataa
     1201 tcaattggat gtcgatattc ccgaggtcag catgaaatca aagtttcgcg gcctggtgct
     1261 cgtag
//
LOCUS       DATCBB010000513         2826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_147208, whole genome shotgun sequence.
ACCESSION   DATCBB010000513 DATCBB010000000
VERSION     DATCBB010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147208"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VII37_05650"
     CDS             complement(<1..330)
                     /locus_tag="VII37_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069246.1"
                     /translation="MAEQSSVIGKDITRIDGILKVTGRAQYGVEYPLENMAWGVGVGS
                     TIGAGRIASIDSAEAEKMPGVLAVLHHANTEKLFRPANSFEESSRPGESRPPFEDDQV
                     YYYGQFVA"
     gene            complement(341..1318)
                     /locus_tag="VII37_05655"
     CDS             complement(341..1318)
                     /locus_tag="VII37_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY5069247.1"
                     /translation="MHEFQYTKANDVGQAVSGATPGGAKFVAGGTTLVDLMKLDVERP
                     KLLVDINGLALDKIEPEGGGLKIGAMVRNSDLAHDEKIMSMYPVLSEALLSGASPQLR
                     NMATTGGNLLQRTRCFYFRDINYPCNKRQPGSGCSAMDGYNRIHAVLGTSPHCIATHP
                     SDMCVAMMALDATIFMTGPKGERSIPITKFYLLPGSTPDRENVLVPGEIITHVTLPAP
                     GQNAKSHYLKRRDRASYEFALASAAVIVELEGRKIRKAQVAMGGVGTIPWRSPEAEHA
                     LAGAETNERAFRKAAEAALHGAKGHKDNGFKIELAKRTLVRALQTVTQA"
     gene            complement(1363..2043)
                     /locus_tag="VII37_05660"
     CDS             complement(1363..2043)
                     /locus_tag="VII37_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5069248.1"
                     /translation="MKENSLDEPPKPEKSDSSTKSPSQLSRRTFLGNIATVGIATTAA
                     PLLQVGELEAQQVHQPATDVAEAPATSVGTVPVSLKINGQTKTLQLEPRVTLLDALRE
                     HLGLFGTKKGCDHGQCGACTVHVNGRRVNSCLTFAIMHQGEEITTVEGLAKNGELHPV
                     QAAFLEHDAFQCGYCTSGQIMSAVALLKEPCGKDDDAIRECMSGNICRCGAYTNIVAA
                     VRSVRGGA"
     gene            2472..>2826
                     /locus_tag="VII37_05665"
     CDS             2472..>2826
                     /locus_tag="VII37_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069249.1"
                     /translation="MPNRVGTQIFRSMDWLLCKTAGITFNTIQHFNKRNPNPSVTPQW
                     SDKPLLKSWEKTKPTLGFPRQTDSLCPACVKEAREAIIAGKKDWRDLMSEKVGEIKAQ
                     IVERDGEVWMVKDCPQ"
BASE COUNT          573 a          728 c          858 g          667 t
ORIGIN      
        1 ggccacgaat tggccgtagt aataaacctg gtcgtcttca aagggagggc gcgattctcc
       61 gggacggctg ctttcttcaa aactatttgc ggggcggaat aatttttcgg tgttcgcgtg
      121 gtgcagaacg gccagcactc cgggcatctt ttctgcttcg gcgctgtcga tgctcgcgat
      181 tcttccggca ccaatggtgc tgccgacgcc gacaccccag gccatatttt ccagcgggta
      241 ttccacgcca tactgcgcgc ggccggtgac tttcaggatg ccgtcaatgc gggtaatgtc
      301 cttgccgatg accgagcttt gttccgccac gcttgctcct ctaagcctgt gtaaccgttt
      361 gtaatgctcg aaccaaggtg cgctttgcta attcaatttt gaagccgttg tctttgtggc
      421 cttttgcgcc gtgtagcgcg gcttccgcgg ccttgcgaaa cgcgcgttcg ttggtttcag
      481 cgccagccag ggcatgctcg gcttccggtg accgccacgg gatggttccg actccgccca
      541 tggccacttg agctttgcga atcttgcggc cctcgagttc gacgattacc gcggcagagg
      601 ccagggcaaa ttcgtaggaa gcgcgatcgc gccgcttcaa gtaatgggat ttggcattct
      661 ggccaggcgc aggcaacgtg acgtgggtaa tgatttcccc gggaaccaga acgttttcgc
      721 ggtcgggagt gctgccaggg agcagataaa atttcgtgat gggaatactg cgctctcctt
      781 tggggccggt cataaaaatg gtggcgtcca aagccatcat ggccacgcac atatcggagg
      841 gatgcgtggc gatgcagtgt ggggaggtgc cgagcaccgc gtggatgcgg ttgtagccat
      901 ccatggcgga gcagccggaa ccaggctggc gtttgttaca cgggtaattg atgtcgcgaa
      961 agtagaagca gcgcgtgcgt tgtaagagat tcccgccggt ggtagccatg ttgcgcaatt
     1021 gcggggaggc gcctgaaagc agcgcttcag agagcaccgg gtacatgctc attatttttt
     1081 cgtcgtgagc gaggtcgctg ttgcggacca tggcgccgat cttcaagccg ccgccttcgg
     1141 gttcaatttt gtcgagcgcg aggccattga tgtcgacgag cagcttgggg cgctcgacat
     1201 ccagcttcat gaggtcgacc agcgtggtgc cgccggcgac aaattttgcg ccgccgggag
     1261 tggcgcccga aaccgcttgg ccgacgtcgt tagctttggt gtattgaaat tcgtgcacgc
     1321 tgacctcttc cagttcttac agcaaaattt ttctgaagat aattacgctc cgccgcggac
     1381 gctgcgcacc gcggcaacaa tattggtata ggccccgcac ctgcagatat tgccgctcat
     1441 gcattctcgt atcgcatcgt catccttgcc gcagggctct ttcaacagcg ccaccgcgga
     1501 catgatctgg ccggacgtgc aatatccaca ttgaaatgcg tcgtgttcca ggaaagcggc
     1561 ctgcaccggg tgaagttcgc cgttcttggc gaggccttcg acggtggtga tctcctcgcc
     1621 ttgatgcatg atcgcgaagg tgaggcagga atttacgcgg cggccgttga cgtgaacggt
     1681 gcaggcgcca cactggccgt ggtcgcagcc tttttttgta ccgaagaggc cgaggtgttc
     1741 gcgcagagca tcaaggaggg tgacgcgcgg ttcaagttgt aaagtttttg tctgcccgtt
     1801 gatctttagc gaaacaggaa cggtgccaac ggatgttgcc ggtgcttcgg ctacgtccgt
     1861 cgccggttga tgcacctgtt gtgcttccag ctccccgact tggagaagcg gggcggcggt
     1921 ggtggcaatg ccgaccgtag cgatatttcc gagaaacgtg cgcctggata actgggaggg
     1981 agacttagtg cttgaatcgc ttttttccgg tttcggcggc tcgtctaaag aattttcttt
     2041 caatgttgcc ctcagtcgaa agtagagttc tacggaaccc ttttgcaaag tcatttatgt
     2101 gcgcaggagg catgcagcgc aatgcaggaa aaaccttaga acgcgggtgg tcaggaagaa
     2161 aagtgcgacc cttcggctgt gataacggtt ccagggctgc ctgcctggta cagattggtt
     2221 tgaacgtact acccatttag tggttggttt aaccatttga cccctgaatc tgaccattca
     2281 ccggaacccg ctggtactgg ggacagttaa cagtcaaact ataggaggtc aaagacttag
     2341 tggtcggcct agctttggat gttaggcttg tagtagtact ttttgagccc gggggcaatc
     2401 gctcgtagct gttcgaacga ttgaatgggc tgaatcccag ttagcagttc gaaagtggag
     2461 ggtgtaggtt tatgccgaat cgagtaggaa cgcagatttt tagatcgatg gattggctcc
     2521 tgtgcaaaac tgccgggatc acctttaata cgatccaaca tttcaacaag cgcaatccga
     2581 acccatctgt tacgcctcag tggtcggata agccgctgct caaatcctgg gagaagacca
     2641 agcccacgct cggtttccca cgacagaccg attcgctctg ccctgcgtgc gtgaaggaag
     2701 cgcgcgaagc catcatcgcg ggcaaaaaag attggcgcga tttgatgagc gagaaggttg
     2761 gcgaaattaa ggcgcagatc gtggagcgcg acggcgaagt ctggatggtg aaagactgcc
     2821 cgcaac
//
LOCUS       DATCBB010000514         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_179221, whole genome shotgun sequence.
ACCESSION   DATCBB010000514 DATCBB010000000
VERSION     DATCBB010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179221"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1274
                     /locus_tag="VII37_05670"
     CDS             84..1274
                     /locus_tag="VII37_05670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5069250.1"
                     /translation="MAEIAIVGGGPSGAMCGEQLARAGNNVIIFDEHLAWEKPCGGGL
                     THKAIQCFPFLLDNPYPKKLVREIDIISSDEHHASLEMTHPIVIYSRTVLNGMLLDRA
                     RAAGCTVQRSHVFNVDTAGSKPRFSVDGECRDTDFLVLAAGARNQLVPETRALQRNEL
                     EMTQGYFVPQTADAITIKFLPHFEGYIWSFPRCDHLSVGICGSMETHTSPELRTHLKT
                     FVEKHKIPTEGAKFYSHVLPSPQEHTLSKRTVLGKNWALIGDAAAWVDPLTGEGLFYA
                     IRSGELLGKALAEGCPEKYPAWIRASFSAELEFASRIVRRFYRGSVLGTAITTRMVQL
                     MRRSPVFRQLVGDLFSGSQDYTSLKRRLWGHLGISVSEFISTVLNLDSPMAAPTARAG
                     TGAD"
     gene            complement(1354..>2029)
                     /locus_tag="VII37_05675"
     CDS             complement(1354..>2029)
                     /locus_tag="VII37_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069251.1"
                     /translation="TLGTDNGAALLAAYAGGNPYAASMVKLNDASPTNSQIFANLGAL
                     GQVATAAAMDSANGTSTLQELIVSKAIAAIDTVAGFAPSDSTNTGLASDLSGSSRVRV
                     AVDLYAVLLLGAVQNQFGITLTAYQSSTGPGVLSGANIPGLLGNRIDASPATPGVQEL
                     KEWMALLSYLGKGLGGSISSEYASTANFTQFGSFGAAVQTRNASYPVASIGQFIGTLS
                     SLQAAP"
BASE COUNT          447 a          640 c          531 g          411 t
ORIGIN      
        1 tccatattag taggctcacc aaacccaccg aatgacgcgt ttcgcatccc gatggatttg
       61 cggcatgcca ggaagatgta gcaatggctg aaatagcgat tgtcggtggt ggtccctcag
      121 gcgccatgtg cggtgaacaa ctcgcacgcg ccggcaataa cgtcatcata ttcgatgagc
      181 atctggcttg ggaaaagcct tgcggcggcg gactgactca caaagccatc cagtgcttcc
      241 cgtttctcct cgataatccc tatcccaaaa aattagtcag agaaatcgac atcatcagca
      301 gcgatgagca tcacgcctcg ctggaaatga ctcaccccat cgtcatttac tcacgcacgg
      361 ttttgaatgg catgctcctc gatcgcgccc gcgccgccgg ctgcaccgtc cagcgctccc
      421 atgtcttcaa tgtggacacc gcgggctcaa agccacgctt ctccgtcgac ggcgaatgcc
      481 gcgacacaga ttttcttgtg ctagccgcgg gcgccagaaa tcaactcgtc cccgaaacgc
      541 gcgccttaca gcgcaacgaa ctagaaatga cccagggcta tttcgtcccc caaaccgctg
      601 acgccatcac cataaaattt cttccacact tcgaaggcta catctggtct ttcccgcgct
      661 gcgatcatct ctccgtcggt atttgcggca gcatggaaac gcacaccagc ccggagctcc
      721 gcactcattt gaaaaccttc gtggaaaaac acaaaattcc caccgaaggc gcaaaatttt
      781 atagccacgt tttaccttcg cctcaggagc acaccctttc gaagcgcacc gtcctcggaa
      841 aaaattgggc gctcatcggc gatgccgccg cgtgggtcga tccacttacc ggcgagggtc
      901 ttttctacgc catccgctca ggagaattgc tcggcaaagc gctagccgaa ggatgccccg
      961 aaaaatatcc cgcctggatc cgcgcttcgt tttccgccga attggaattc gcgtcgcgca
     1021 tcgtgaggcg tttttatcgc ggctccgttc taggcaccgc catcaccacc cgcatggtgc
     1081 aactaatgcg ccgcagcccc gttttccggc aattggtcgg agatctgttc agcggcagcc
     1141 aggattacac cagcctcaag cggcgcctgt ggggccatct gggaatttcc gtcagcgaat
     1201 ttatctctac ggtgttgaac ctcgacagcc ccatggccgc acccactgcc cgcgcaggga
     1261 ccggcgccga ctgagcagca cctcgatttc cgcacggaag cgtaccggtg cgagtttcga
     1321 caaaaaccaa cattccatag ccacatttcg attctaaggg gcagcctgca ggctgctgag
     1381 agtgcctatg aactgcccga tgcttgcgac agggtagctg gcattccgtg tctgaacagc
     1441 cgcgccaaag ctgccaaatt gggtgaaatt ggcggtcgac gcatactcgg agctgattga
     1501 cccaccgagg cccttcccca agtaggaaag aagtgccatc cactccttga gttcctgaac
     1561 gcccggagtg gccggcgatg cgtcaatgcg attacccaac aaacccggga tatttgcgcc
     1621 ggataagaca cctggcccag tcgacgattg gtacgccgta agggtgatgc cgaactggtt
     1681 ctgaaccgcc cccaaaagca gcacggcgta caagtcgact gccacccgca cacgcgagct
     1741 acccgaaaga tcactggcga gtccggtgtt agtgctgtcg ctcggcgcga agccggccac
     1801 cgtgtcaatc gccgcgatcg cctttgagac gatcagctct tgcaacgtgg aggtgccgtt
     1861 ggcgctatcc atcgcggcag cggtggccac ctgacccaag gcgccgaggt tggcgaatat
     1921 ctgcgaattg gtcggactcg catcgttgag ctttaccatg gatgcggcat atggattccc
     1981 gccggcgtac gctgcaagca acgcagcgcc gttatccgta cctagagtc
//
LOCUS       DATCBB010000515         1516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_49901, whole genome shotgun sequence.
ACCESSION   DATCBB010000515 DATCBB010000000
VERSION     DATCBB010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49901"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1151)
                     /locus_tag="VII37_05680"
     CDS             complement(126..1151)
                     /locus_tag="VII37_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5069252.1"
                     /translation="MAHEWPTLLGDNARSGGQAPQPWRAPGRARWQTNVEGAVRGSPV
                     LDGDLLYVPSVAGYLNAINIRSGRLQWRYKAPAAIHCTPSISSGRVLFGCSDGKVIAI
                     DAVTGSKSWETTAEDEVWTSPVVRNKTVFFGSADGTMYAVQLESGTLKWKEGLGGRIY
                     STAHVAEQHVYFGCGDGNIYALDALTGHVVWKTATGNGIDCSPALAHDTLLIGSQDFF
                     FYALDAGSGAIRWKYEAELGITSAPAISGDLVIFGCKAGTLHALDIRTGKVIWKTQTG
                     QAITAPPVVSGSVIWLQAEITFALNVPDGKVIWRAGMGNSLHSAPVVTNQAVYLAGME
                     GAVYALE"
     gene            complement(1195..>1516)
                     /locus_tag="VII37_05685"
     CDS             complement(1195..>1516)
                     /locus_tag="VII37_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069253.1"
                     /translation="RMVWHGGPLTQNREFIFPAGLIMIGTDPVAMDTIELEKIEAKRS
                     KEGVPSVWSRDPTSLTNDDEAFYQDASKNFFYRQPHHIAAAGKLGLGISDRKQIELQA
                     IRIT"
BASE COUNT          333 a          458 c          392 g          333 t
ORIGIN      
        1 aggcgacaat ctcgaacagc gtccccatgg cgtaacgctt cttgtgaaca aaccgtgtgg
       61 tctggctgga tacatccggc acatgaataa gaaccgacga aatcagcgct gcggcaagca
      121 gaaacctatt ccagcgcata gactgctcct tccatgccgg caaggtacac ggcctggttt
      181 gtcaccaccg gcgccgaatg caaggaattt cccatgccgg ccctccagat aacttttcca
      241 tcgggcacgt ttagtgcgaa agtgatttcc gcctgaagcc aaatcaccga tccgctgaca
      301 acagggggcg cggtaatcgc ttgacccgtt tgggttttcc aaatgacctt tcccgtccta
      361 atgtccaacg catgcaaagt tccagctttg catccaaaaa tgacgaggtc gccggatatg
      421 gcgggtgccg aagtgatgcc caattcggcc tcatatttcc agcgaatcgc tccgctgccg
      481 gcatccagcg cataaaagaa gaaatcctgc gagccaatca gaagcgtgtc gtgggccaga
      541 gccggcgaac aatcaatgcc gttcccggtg gcagtcttcc aaacgacgtg gccggttagg
      601 gcgtccaagg cgtagatgtt gccatctcca cagccaaaat agacatgttg ttcagcaacg
      661 tgcgccgtcg aatagattct gccgcccagc ccctctttcc atttcagtgt gccgctttcc
      721 aactgcaccg cgtacatagt cccgtcagca ctcccgaaga acaccgtttt gtttcgcacc
      781 actggcgaag tccacacctc gtcttctgcc gttgtctccc aagacttaga gccggtgacc
      841 gcgtcaatgg cgatcacctt gccgtcgctg cagccgaaca gtacgcggcc agaactaata
      901 gaaggagtgc aatgaatggc agcgggtgct ttgtatctcc actgcaaccg cccgctacga
      961 atattaattg cattcagata tcccgccacc gacggcacgt aaagaaggtc accatcgagc
     1021 accggcgagc cgcgtaccgc tccttcgacg ttggtttgcc aacgcgccct gccaggcgca
     1081 cgccacggtt gtggagcctg cccgccgctg cgggcattat caccaagcaa ggttggccat
     1141 tcgtgtgcca tccgcgtcta agtaaccttt ctgcatccct gcgaggcgtt tccctcaggt
     1201 gatgcggatc gcctgaagtt caatctgttt acgatcggat attcccagcc cgagtttgcc
     1261 cgccgccgcg atgtgatgag gttggcggta aaaaaaattt ttcgaagcgt cctggtaaaa
     1321 cgcttcgtca tcattcgtaa ggctggtggg atcgcgggac cacacgctcg gcacgccttc
     1381 tttgctccgt ttggcttcaa ttttctccag ctcgatcgta tccatggcaa ccggatcggt
     1441 cccaatcatg atcaggccgg cggggaaaat aaattcgcgg ttttgggtaa gcgggccgcc
     1501 gtgccagacc attcgt
//
LOCUS       DATCBB010000516         4674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_341183, whole genome shotgun sequence.
ACCESSION   DATCBB010000516 DATCBB010000000
VERSION     DATCBB010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341183"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VII37_05690"
     CDS             <1..290
                     /locus_tag="VII37_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069254.1"
                     /translation="LIEGDFRITATELDAMLRLNLIHRSGVEAQNIEGIVELMGGARC
                     GERRLRCCEAEQYEKGCCEPHLESVVILGAVEQGKCLQDLLIPGMRDFALD"
     gene            431..1891
                     /locus_tag="VII37_05695"
     CDS             431..1891
                     /locus_tag="VII37_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069255.1"
                     /translation="MGSLSILRGCQAGVFSLALALGGAGVWADTIVLKNGHRISASNV
                     VEVGDKIRYETSVGELSLPKAIVDHIEKGGAGPRSASSSDNASNMAITPPVMDAPGSN
                     GGEIERAAIHDGSIDREYIAKLESETRSGSPLAGINAAVAHHAAANFELAHGDMDHAL
                     ADERTALTYAPEGPLLLMNVAYLHLRRSEYRESLDYLERAQRIAPNNPDVAKLAGWAY
                     YGLNKLPQAVAEWKRALALRPDAEVQAALEKAERDKGEEESYKENESAHFTLRYSGSA
                     EPALAREVLRTLEMHYARINSELNFSPPDSIGVILYTQQAFADITRAPGWVGALNDGR
                     LRIPVQGLSSMTDELSRVLKHELTHSFVQQKTHGRAPTWIQEGLAQWMEGKRSGDNAA
                     VLLDIYGDGHATSLRSLEGSWMAFPSNAAVYAYAWALANIEYIVQADGMGDVERILDR
                     IGSGSSTEAALHDVLHSDYDDVMRSSADYLRRTYSR"
     gene            complement(1932..3029)
                     /locus_tag="VII37_05700"
     CDS             complement(1932..3029)
                     /locus_tag="VII37_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY5069256.1"
                     /translation="MVDTLRRFVIAESPSLEKAYADRCCEIIATEWRKHGLQVRRIAQ
                     NNRGDHLHITCTPNSSRPKGQLLVLGHYDTVYASGTLKKMPFRLTAGKAYGPGTFDMK
                     AGIVQALFGYHALQKLNHVPNKRIVFLWTSDEEIGSDSSRKLIETEARRSDAVFVLEP
                     SYGPRGLIKTARKGVGEAEIIVHGRASHAGLAPEEGINAVHELCLQVSSILKWNNPRR
                     GTTVNADIIQGGTRTNVIADRARAVLDLRATSISEMTRLERQLHALKPHLRGARLEIH
                     GGFSRSPLERKMSAALFKRAKSLAAQMNLTLGECVVGGGSDGNLTAALGIPTLDGLGA
                     VGDGAHSTHEHILTAAMPIRAALLAALLATS"
     gene            3316..3816
                     /gene="fabZ"
                     /locus_tag="VII37_05705"
     CDS             3316..3816
                     /gene="fabZ"
                     /locus_tag="VII37_05705"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY5069257.1"
                     /translation="MILDTTEIQRILPHRYPFLMVDAIEEMERVKRIVGIKNVTINES
                     HFQGHFPGKPIMPGVLIIEAMAQTGGLLLLMEVPEREREQKLLYFVAIDGARFRRPVV
                     PGEQLKLEVKVISWRGDFCKLDGKATVNGELAAEAILMCKMVDREDLEKPKTAKQINS
                     SGNSHD"
     gene            3968..>4674
                     /gene="lpxA"
                     /locus_tag="VII37_05710"
     CDS             3968..>4674
                     /gene="lpxA"
                     /locus_tag="VII37_05710"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY5069258.1"
                     /translation="MSTQTKTEIHPQAVVSASARLGERVKIGAYAIVGEHVTLGDGCS
                     VDAHAVIQGPSTIGRRNVFHPFCSIGGDPQDLKFHGEQTELTIGDGNTFREYVTVSRG
                     TVGGGGKTTIGNGSLFMAYSHIAHDCHVGNETIFANGATLAGHVEVEDFATIGAFSPV
                     HQFCRIGRYAYVGACTVITQDVPPFSRVVTERETKCYGVNTVGLERRGFDRARLETIE
                     RAFRLLLRSKLNTTQAL"
BASE COUNT         1034 a         1053 c         1563 g         1024 t
ORIGIN      
        1 ccttgatcga aggagatttt cgcatcaccg cgacggagct cgatgcgatg ctcaggctta
       61 atttgataca ccgaagcggg gtcgaggcgc agaacattga gggcatcgta gagctgatgg
      121 gcggtgcgcg ctgcggagag cgaaggttgc gctgctgtga ggcagaacaa tacgaaaaag
      181 gctgctgcga gcctcatctg gaaagtgtag tcattttggg cgctgtggaa caagggaagt
      241 gcctgcagga tttattgatt cccggaatgc gagacttcgc tctggattga cgtattacgg
      301 accgaaggga tagcagacgc ggtccaaacg ccgattctga ctggcgttgt ttgaaggagc
      361 gcctttgcgc tactggaaca acgtcgtgcg agaaaaattc aaagggttga ggtgcgggta
      421 aaattggggc atggggtctc tctctatttt gcgcggctgt caagctggcg tgttcagttt
      481 ggcgttggca ctcggcggcg cgggagtgtg ggcagacacc attgtattga agaacgggca
      541 ccgcatttcc gccagcaatg tagtggaggt gggcgacaag attcggtatg aaacgagtgt
      601 aggggaactg agcctcccga aggcgattgt cgatcacatc gaaaaaggcg gcgcggggcc
      661 acggtctgca tcgtcaagcg ataacgcttc gaatatggcc attactccac cggtcatgga
      721 tgcgccgggt agtaacggtg gggaaattga gcgcgcagcg atacacgacg gctcgattga
      781 ccgggaatat attgcgaaat tggaatctga aacgcgcagc gggtcgccgt tggcggggat
      841 caatgccgcg gttgcgcatc atgccgccgc gaattttgaa cttgcgcacg gggacatgga
      901 tcatgcgctg gctgacgaac gtacggcact tacttatgcg ccagagggac cactgctgct
      961 tatgaacgta gcctatttgc atttgcgcag aagtgagtac cgggaatcac tggattatct
     1021 ggaacgcgcg cagcggattg ctcccaacaa tccagacgtg gccaagctgg cgggttgggc
     1081 ttattacggg ctgaataagt tgccgcaggc ggtggcggaa tggaagcggg ctttggcgtt
     1141 gcggccggat gcggaagtgc aagcggcgct ggaaaaagcg gagcgcgaca agggagaaga
     1201 agaaagctac aaagaaaatg agagcgcgca ttttacgctg cgctacagcg gaagcgcgga
     1261 gccggcactg gcgcgagaag tgctgcgaac tctggagatg cattacgcgc ggattaattc
     1321 tgagctgaat ttttcgccgc ctgattccat tggtgtgatt ttgtatacgc aacaggcttt
     1381 cgcggatatt acgcgcgcgc cgggatgggt gggggcgctg aacgatggaa gactgcgcat
     1441 tccggtgcag ggattgagca gcatgacgga cgagctttcg cgggtgctga aacatgagct
     1501 gacgcacagt tttgtgcagc agaagacgca tgggcgggcg cctacctgga tacaggaagg
     1561 gctggcgcag tggatggaag gcaagcgcag cggggacaat gccgcggtgc tgctggacat
     1621 ttatggcgat gggcacgcga cttctctgcg gagtttggaa ggctcctgga tggcatttcc
     1681 gagtaatgcg gcggtttatg cgtatgcctg ggcactcgcg aatattgaat atattgtgca
     1741 ggctgatggc atgggagacg tggaacgaat actggaccgg attgggtcgg gaagttcgac
     1801 ggaagcggcc ttgcatgatg tgctgcatag cgattacgac gacgtgatga gatcgagtgc
     1861 ggattatttg cgcagaacgt actcgcgtta gggatagact ttcctttttc gaaaacacga
     1921 taagcgcagt tttaactagt ggcgaggagc gcggctagca gcgctgcgcg gatgggcatg
     1981 gcagcggtca ggatgtgctc atgagttgag tgcgcgccat cgcccacggc gccgaggcca
     2041 tcgagcgtgg ggattcccaa cgcggcggta agatttccgt ctgagcctcc gcctacaaca
     2101 cactccccca gggtcaagtt catttgggca gcaagggatt tcgcgcgttt gaataaggct
     2161 gcgctcattt tgcgctcgag gggagagcga gagaatccgc cgtggatttc gagtcgggca
     2221 ccgcggagat gtggcttcaa ggcgtggagt tggcgttcga ggcgggtcat ttcggagatg
     2281 ctggtggcgc gaaggtcgag caccgcgcgg gcgcgatcgg cgatcacatt tgttcgagtg
     2341 ccgccctgga tgatgtcagc gtttacagta gtgccacgac gcggattgtt ccatttcaaa
     2401 atgctggaca cctggagaca gagctcgtgt acggcgttga tgccttcttc cggagccaag
     2461 ccagcgtgag aggctcggcc gtgaacgata atttccgctt cgccgactcc cttgcgcgcg
     2521 gtttttatca ggccgcgggg gccgtacgag ggctccagaa cgaaaaccgc atcgctgcgg
     2581 cgcgcttcgg tttcgatgag tttcctggag gaatcgctgc cgatttcttc gtcagaggtc
     2641 caaagaaaaa caatgcgttt gttggggacg tggtttagtt tctgaagtgc gtggtacccg
     2701 aagagggctt gcacgattcc agccttcatg tcgaaggtgc ccggtccgta ggctttgccg
     2761 gcggtgagcc tgaaaggcat tttttttaag gtgccggagg catatacggt gtcgtaatgg
     2821 ccgaggacga ggagttggcc tttgggtcga gaagaattgg gggtgcaagt gatgtgcaaa
     2881 tgatcaccgc gattgttttg cgcgatgcgg cgaacttgca gaccatgttt gcgccattcg
     2941 gtggcgatga tttcgcaaca gcggtcggcg taggcttttt ccagactggg cgattcagcg
     3001 attacaaaac ggcgaagcgt atcgaccatt tggggaagcg ctggttgcag gaagtttttg
     3061 attttctgag cgaccgaggg agcgggcatt gtgcgaacat gctaacaatg aggcgccgaa
     3121 tgggcaacga agcgagtagc catatcaagt gaacaggtag gcgggcgtac cggaggcgat
     3181 tggcgccatt ctggagatat tgcatttggg gcacaagata cgccgtcatt gtggggcgtt
     3241 ggtcgcatat aattgtaagt ttgccgggta agaccggacg ccgcagggtc tgacgtattc
     3301 gcgacggggg tgcgaatgat tctggataca acagaaatac aaaggatttt gccgcaccgt
     3361 tatccatttc taatggtgga cgccattgaa gagatggagc gcgtgaagcg catcgtggga
     3421 atcaagaatg taaccatcaa tgagtcgcat tttcaggggc atttcccggg taagccgatc
     3481 atgcccggag tgctgatcat tgaggccatg gcacagacag gcggtttgct gttgctgatg
     3541 gaagtgccgg agcgggagcg cgagcagaag cttttatatt ttgtggcgat tgatggggcg
     3601 cgattccggc gaccggtagt tcccggggag caattgaagc tggaagtaaa agttatttcg
     3661 tggcgtgggg atttttgcaa gctggatggt aaggcgacgg tcaacgggga gctggccgct
     3721 gaagcgatat tgatgtgcaa gatggtggat cgcgaagacc tggagaagcc taaaactgcc
     3781 aagcaaatta attcgtctgg taactcgcac gattaattga aagcagccac gagaaattca
     3841 tacgacacgg gtaacgcccc tgtgtggatc gcagtgttat tgccaagatt gatgatgcga
     3901 atgaacgcca ctcggtacga cgaaccgtgt gacacgtgtg acggcgatac ctaagggcta
     3961 aaaatttgtg agcacacaga ctaaaactga gattcatccg caggcggtag tgtccgcgag
     4021 cgccagactt ggtgaacggg taaaaattgg agcttatgcg attgttggcg agcatgtgac
     4081 tttgggcgac ggctgttccg tggatgcgca tgcggtaatt caagggcctt cgacgattgg
     4141 gcggcgaaat gtgtttcatc cgttttgttc gatcggcggg gaccctcagg atttgaaatt
     4201 tcatggcgaa caaacggaat tgacgattgg tgatggaaac acgtttcggg agtatgtgac
     4261 ggtcagtcgc gggactgttg gcggcggagg gaaaacaacc atagggaacg ggagtttatt
     4321 tatggcctac tcgcatatcg cgcatgattg ccatgtcggc aacgagacga ttttcgccaa
     4381 tggggcgacg ctcgcgggac atgtagaggt ggaagatttc gctacgattg gcgcgttttc
     4441 gccggtgcac cagttttgcc gcattgggcg ctatgcgtat gtgggggcct gtacggtgat
     4501 tacgcaggac gtgccgccgt tttcgcgcgt ggtcaccgag cgcgaaacga aatgctacgg
     4561 cgtgaacacc gtcggcctcg agcggcgcgg attcgaccgc gcgcgtctgg aaaccatcga
     4621 gcgcgcgttt cgccttctgc tccgctccaa actcaacacc acgcaggcgc tcga
//
LOCUS       DATCBB010000517         1717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_211403, whole genome shotgun sequence.
ACCESSION   DATCBB010000517 DATCBB010000000
VERSION     DATCBB010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211403"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VII37_05715"
     CDS             complement(<1..894)
                     /locus_tag="VII37_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5069259.1"
                     /translation="MSSPPGLITITIDGRAVAVPPGTSVFDAARIHGIAIPTLCHLQN
                     ETPVGVCRLCVVDTGARVLSASCVRPVEPGMKVETNSGKVLATRKTVLELLMADHPSP
                     CARQQKSGDCELETLAKSAGIEQPRFAKRSVARGLDDSSLAIAVDHDACILCDRCIRG
                     CDEIKNNFVLGRMGKGYSAGIAFDLNAPMGNSTCISCGECMVSCPTGALTNKGVAGTS
                     IAAADDGYTFQAEELLRIPVFKGVSGTFLELNRGAIVKRRYRKGALICREGEFGSTAF
                     YIVEGTAKVSISSPIAHVKTHG"
     gene            complement(951..>1717)
                     /locus_tag="VII37_05720"
     CDS             complement(951..>1717)
                     /locus_tag="VII37_05720"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5069260.1"
                     /translation="KPPFPVQAGLFQKPTVLNNVETFANVPQILARGVEWFKSQGQGG
                     SRGLKFVGVSGHVANPGIFEVPTGIPMREVLFKYAGGIRGGRTLKAFAPSGPSSGYLP
                     ASMVDVRLDFKALADAGSMLGSGAIVVCDDTTCMLDMALNAVRFYRNESCGKCVPCRV
                     GSQKMVDLLMRWAQGALPEAQYRADLALLDELSLAMGLTSICGLGQIVPAPIQSVLKH
                     FRAEIDAHVLKGLCPSGICFTEASRSIETQRVGIRP"
BASE COUNT          398 a          478 c          490 g          351 t
ORIGIN      
        1 gccgtgagtt ttcacgtgtg cgatggggct ggaaatcgaa actttcgcgg tgccttccac
       61 aatataaaac gcggtggatc cgaattcgcc ttcgcgacag atcaatgcac ctttgcggta
      121 acgcctttta acaattgcac cgcgattgag ttccaggaat gtgccggata cgcccttgaa
      181 aacgggaatg cgaagaagtt cttcggcttg aaatgtatat ccgtcgtctg cggcggcgat
      241 ggaagtgcca gcaacgcctt tgttcgtgag cgcgcccgtg gggcaagaaa ccatgcattc
      301 gccgcacgag atgcacgtgg agtttcccat gggagcgttc aaatcgaagg cgatgccggc
      361 cgaataccct ttgcccatgc gtccgagcac aaaattgttt tttatctcgt cgcagccacg
      421 aatgcagcga tcgcacagga tacacgcgtc atgatcgacg gcgatggcca gcgaggaatc
      481 atcgaggcca cgcgcgacac tacgtttggc gaaacgaggc tgttctatgc ccgcggattt
      541 cgccagcgtt tccagttcgc aatcgcctga tttttgctgg cgcgcgcacg gcgatggatg
      601 gtcggccatc aacaattcaa ggaccgtctt gcgcgtggcc aagaccttgc cagaattggt
      661 ttccaccttc atacccggtt cgacgggacg aacgcaggaa gcgctgagaa cgcgtgcacc
      721 ggtgtcgacc acgcagagcc ggcacacgcc tacaggagtt tcgttttgca gatggcacaa
      781 cgtgggtatg gcaataccgt gtatccgcgc cgcatcgaaa acactcgttc cgggaggaac
      841 ggccaccgct cgaccatcga tagtgatggt gatgagcccg ggaggtgaag acacgctagc
      901 cattatttag ttggttcgcc ggtcatagtt acaaaagcgg gctgcatgcc tcatggccgt
      961 atacctacac gttgcgtttc gatcgagcgc gatgcctcgg taaagcatat tccactaggg
     1021 catagccctt tcaaaacgtg tgcgtcgatt tcggcgcgaa aatgtttcag cactgactga
     1081 atgggcgctg gaacaatttg cccgaggccg cagatggacg tgaggcccat cgccaaagac
     1141 agttcgtcaa gcagagccag atcggcccga tattgtgctt ccggcagtgc gccttgggcc
     1201 caacgcatca gcaaatccac cattttttgc gagccgaccc ggcaaggcac acacttaccg
     1261 caagactcgt tgcgatagaa gcgcaccgcg ttgagagcca tgtccaacat gcaagtagtg
     1321 tcgtcgcaga ccacaatggc gccagagccc agcatggacc cggcgtcggc aagagctttg
     1381 aaatccaggc gcacgtcgac cattgacgcg ggcagataac ccgaagaagg ccctgaaggc
     1441 gcaaaggctt tcaaagtgcg gcctccgcga atgcctccgg cgtatttgaa aagaacttcg
     1501 cgcatgggta tcccagtggg gacttcaaaa atgccgggat tggcgacgtg gccgctgacg
     1561 cccacaaact tcaacccgcg cgagcctcct tggccctgcg atttaaacca ttccacgccg
     1621 cgagcgagaa tttgcggcac gttcgcgaaa gtttcaacgt tgttcagaac ggtcggtttc
     1681 tgaaacaagc cagcttgaac agggaaaggt ggtttgt
//
LOCUS       DATCBB010000518         1526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_49941, whole genome shotgun sequence.
ACCESSION   DATCBB010000518 DATCBB010000000
VERSION     DATCBB010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49941"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VII37_05725"
     CDS             complement(<1..1196)
                     /locus_tag="VII37_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5069261.1"
                     /translation="MKKHEDHIQRLPEFNVTAWDRSRQRLHAGAILFAAFAIAAGAPV
                     WAQDVAEAARQAQARKHQQTKHKKHVYTEEDLKRARILTREDQELLAAKQRNAVIPGA
                     EAPTTLDAQSLEHLPLGDVARMYRAMKELNHSQTGELAEYHLPVANAELASANVEFIV
                     PKPNAVTPLPYNTPAAPFFPNTEVNTPTLNTLLVSPKPNIPAPTPNRLTAAKLRSMAR
                     TVSADAKFAASRPNMLVRSAHEAPVAPAFSNADLISTPDLTFAAFRPTTQPAPTAPAF
                     SNASGPRSIIVKYGDSLWKLAQMNLGDGHRWHELAAINPEIVNPNYILPGTPINIAGT
                     APAPPASPSEPRVAVRKGDSLWKIAQKKLGFGGFWGCIAKANPVIRDANRIYAGQVLA
                     VPETCDT"
BASE COUNT          321 a          392 c          461 g          352 t
ORIGIN      
        1 gtgtcgcagg tctcgggtac cgccagaacc tgtccggcat agatacggtt tgcatcgcga
       61 atcacggggt tagcttttgc aatgcagccc cagaatccgc cgaagcctag ctttttctgg
      121 gcaattttcc acaggctgtc gcccttgcgt accgccactc tgggttcgga gggggacgct
      181 ggcggagccg gcgcagttcc cgcgatgttg attggcgtgc caggcagaat gtagttggga
      241 ttaacgatct caggattaat ggcggccagc tcatgccagc ggtggccgtc gcccaaattc
      301 atttgcgcca gcttccacaa cgaatcgcca tatttcacaa tgatgctgcg cggccccgat
      361 gcattagaaa acgcaggagc agtcggcgcc ggttgagtgg ttggtcggaa cgcagcaaac
      421 gtcaggtccg gcgttgaaat cagatcggcg ttcgaaaacg ccggagcaac tggcgcttca
      481 tgcgctgatc gtacaagcat gttcggtctt gaagcggcaa atttcgcgtc ggccgaaact
      541 gtcctcgcca tggaacgcag cttagcggcg gtcagacggt taggtgttgg cgctgggatg
      601 tttggttttg gcgagaccaa tagggtattc aacgtcggag tattcacctc cgtatttggg
      661 aaaaagggcg ccgcgggtgt gttatacggc agtggcgtta cagcgttcgg cttcggcacg
      721 atgaattcca cgttcgcaga tgcgagctcc gcgttggcaa caggcagatg atattctgcg
      781 agctctccgg tctgcgagtg attcaactct ttcattgcgc ggtacatgcg agccacatcg
      841 ccgagcggga ggtgttccaa cgactgcgcg tctaaagtgg taggagcttc ggcgccgggt
      901 attactgcgt tgcgctgttt cgcggccagc aactcctgat cttcgcgggt aagaattctt
      961 gcgcgcttca ggtcttcttc cgtatagacg tgctttttat gcttggtttg ctgatgttta
     1021 cgagcttgag cctgacgtgc cgcctccgcg acatcctgcg cccagaccgg cgctccggcg
     1081 gcaatggcga atgctgcgaa caaaatagcc ccggcatgca ggcgctgcct agagcgatcc
     1141 caggcggtga cattaaattc cggtaatctt tggatgtggt cttcgtgttt tttcatggca
     1201 taccccaaga attaagcttg gagccagttt atcgaaggct ggagtgcgtc aaaaaatcga
     1261 ttcgcgagag tgttctgttc gctatagaaa cattcgacac caaaatatcc gcattggaac
     1321 aggttgaggc ggggcttttg gggaattgcc accagtgcca taacggtacc aggcacagcg
     1381 catagagttt cccgtggctg cacggagggt gaacttgacg taacatgtct ccgcccaagt
     1441 tactttcacg aggtcacaaa tgaaggatga agcagacgta gacggcgaaa cgacgttgcg
     1501 caagactgcg cggcagaagg aacgcg
//
LOCUS       DATCBB010000519         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_438317, whole genome shotgun sequence.
ACCESSION   DATCBB010000519 DATCBB010000000
VERSION     DATCBB010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438317"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VII37_05730"
     CDS             complement(<1..1128)
                     /locus_tag="VII37_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5069262.1"
                     /translation="MYTELHARSAFSFLAGASLPEELASVCAEREMEAMAVLDRDGMY
                     GAPRFYMAAKKSSVRALIGAEVTAEEGWRYPLLVESRKGYQNICRLITSMKLRAKKGE
                     GQISRAELAAHLGNDGGGLICLSGGDEGPLAHALASGGMEAAMSSVQQLCELFGRRNV
                     YVELQRHYCRDEEVRNQCALEIARKLNLPLLATNGVCHALPEQRELLDVFSCIRHHRT
                     LATAGRLLARNAERHLKSGAEMAELFADLPEAIANTQVLASRLQFTLNDLGYQFPIYP
                     VPDGRSQMQFLRDRTYDGMLARYGTDNERARKQIAHELDVIEKLDLAGYFLIVWDIIR
                     FCGQQNILVQGRGSAANSAVCYALGITAVDPVGMELLFERFL"
     gene            complement(1128..>2483)
                     /locus_tag="VII37_05735"
     CDS             complement(1128..>2483)
                     /locus_tag="VII37_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069263.1"
                     /translation="APDTIVLDLAGLSGLFGAEEKIAHELAKRGSGLGLSARIAVAKN
                     IEVAILASRGLPGVTLIPPGEEANRLKTLPVHTLLPSPEILETLTRWGVETCDKLAAL
                     PVIQLSERLGQPGVRLHTLARGALVRSLVLAKPSGNFQEEMELDDSVEELESLSFMLG
                     RLLDQLCERLSARSLSARSIRMEFDLERSFEKGLQISAHANQHKLIPRKFEKTLALPV
                     PMRDSKLLLKLVRLRLQSDPPPAAVIKILLCAEADRVRTTQRGLFLPASPEPEKLELT
                     MARLANLVGDANVGSPQLMDTHRPDAIRMERFRLAQEAVGGRKKKPIAAVNKSVDKNS
                     SASRMAKPANGFRLLRPSVPARVELQDGLPVRVFFQGARGEIVAASGPWRTSGEWWQD
                     PWDQAEWDVEVRFSASPQKNKRSSGPSSNSSLNPGSERGLYLIYYDLRQQGWFLRGMY
                     D"
BASE COUNT          570 a          802 c          592 g          519 t
ORIGIN      
        1 caaaaaccgt tcgaacagca gctccatgcc caccggatcc accgccgtaa ttcccagcgc
       61 atagcaaacc gcgctgttcg ccgccgagcc acgcccctgc accagaatat tttgctgccc
      121 gcaaaaacga atgatgtccc agacaatcag gaaatagccc gccagatcga gcttttcaat
      181 cacatcaagt tcgtgcgcga tttgtttgcg cgcgcgctcg ttgtccgttc cgtagcgcgc
      241 cagcatcccg tcgtaagtgc ggtcgcgcaa aaattgcatc tgcgagcgcc catccggcac
      301 gggatatatg gggaattggt aacccagatc gttcaacgta aactgcagcc gcgaagccag
      361 cacctgcgtg ttggcgatgg cttccggaag atcagcaaaa agttccgcca tctccgcacc
      421 ggatttcaga tgccgttcgg cgttgcgagc cagtagccgc ccggcggtag ccagcgtgcg
      481 gtgatgtcta atacaagaaa atacatccag caattcacgc tgctccggca aagcgtgaca
      541 aacgccatta gtagccagca gcggcagatt taatttgcgc gcgatttcca gcgcgcactg
      601 gttgcgcacc tcttcgtcgc ggcaataatg ccgctgcaat tccacatata cattgcgcct
      661 tccaaataac tcacaaagct gctgaacgct gctcattgca gcttccattc ccccggacgc
      721 cagcgcatgc gcgagcggcc cttcatctcc cccggagaga caaatcaatc caccaccatc
      781 atttcccaga tgcgcagcta attccgcgcg cgagatttgc ccttcccctt ttttcgcgcg
      841 caatttcatg ctggtgatca agcggcaaat attttgataa cctttacgcg actccaccag
      901 taacgggtat cgccatcctt cttcagcagt aacttccgcc ccgatcagcg cgcggacact
      961 tgatttcttc gccgccatat aaaaacgtgg cgccccgtac atgccgtcgc gatcgagcac
     1021 cgccatcgct tccatttcgc gttccgcgca cacactggcc aattcttccg gcagagaagc
     1081 ccccgcaaga aagctgaacg cggaacgtgc atgcaattcg gtgtacatca gtcgtacatt
     1141 cccctcagaa accacccttg ctgccgcaaa tcgtaataaa tcaggtacag tccgcgttcg
     1201 ctgccaggat ttaatgacga atttgaagac ggacctgaag aacgtttatt tttttgcggg
     1261 ctcgctgaaa agcgaacttc cacatcccat tccgcttgat cccaggggtc ttgccaccac
     1321 tctccagaag ttcgccatgg cccagacgcc gccacgattt cgccgcgcgc gccctgaaaa
     1381 aatacccgca ccggaagccc atcctgcaat tccacacgcg caggaacgct ggggcgtaaa
     1441 agacgaaacc cattcgcggg cttcgccatt cgcgatgctg aagaattctt gtccacagat
     1501 ttgttcaccg ctgcaattgg cttttttttg cgaccaccga ccgcctcctg cgccagtcgg
     1561 aaccgctcca tgcgaatcgc atccggacgg tgcgtatcca tcagttgtgg cgagcccaca
     1621 ttcgcgtcgc ccaccagatt tgcgagccgc gccatggtca gctccagctt ttcaggctcc
     1681 ggcgaagctg gcaaaaataa tccgcgctgt gtcgtacgca cccggtccgc ttcggcgcat
     1741 agcaaaattt ttataaccgc tgcaggcggt ggatcggact gcaagcgcaa ccgcaccaat
     1801 ttcagtagca atttggaatc ccgcatcggc accggcagcg ccaaagtctt ttcaaatttg
     1861 cgtggaatca gtttgtgttg attggcatgc gcggaaattt gtaaaccctt ctcgaacgaa
     1921 cgctccagat cgaattccat tcgaatgctg cgcgccgaca gcgatcgcgc cgacagccgt
     1981 tcgcacaatt gatccagcaa acgccccagc ataaagctca gcgattccag ttcctccacg
     2041 gaatcatcca gctccatttc ttcctgaaaa ttacccgaag gcttggccag caccaacgaa
     2101 cgcaccaaag cgccgcgcgc cagcgtgtgc aaacgtaccc cgggttgccc cagccgctca
     2161 gaaagctgta tcaccggcaa cgctgcgagc ttgtcgcagg tttccacgcc ccagcgagtc
     2221 aatgtctcca gaatttcagg cgaaggaaga agcgtgtgca ccggcaaggt cttcagccga
     2281 ttggcttctt cccccggcgg aattaatgtg acacccggca acccgcgaga cgccaggatc
     2341 gccacttcga tatttttagc taccgcgatg cgcgcgctta aacccagccc ggaaccgcgc
     2401 ttcgcaagtt catgagcgat tttttcttcc gcgccaaata aaccgctcaa tcccgcgaga
     2461 tccagaacta tggtgtctgg cgc
//
LOCUS       DATCBB010000520         1384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_276412, whole genome shotgun sequence.
ACCESSION   DATCBB010000520 DATCBB010000000
VERSION     DATCBB010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276412"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VII37_05740"
     CDS             <1..225
                     /locus_tag="VII37_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069264.1"
                     /translation="IAIQALVEGLRSATVFIPASVGIQEAGYAALAPVFGLTPEIGLA
                     VSLLRRARDIVVAVPVLLIWQLIEGRRAAR"
     gene            813..1139
                     /locus_tag="VII37_05745"
     CDS             813..1139
                     /locus_tag="VII37_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069265.1"
                     /translation="MGFPVSDMACSIVRQLEILFCNDEGRGIDINDCYLSSRSHKHSS
                     QRAAAAAYQKNPAGPGLRNKPEDRMNIGGEAYAVTVCFTLAAAALFISNRARRFSFEN
                     RDRSEV"
BASE COUNT          296 a          399 c          391 g          298 t
ORIGIN      
        1 atcgccatcc aggcgctggt tgagggcttg cgcagcgcca cggtgttcat tcccgcctcg
       61 gtgggcatcc aggaagccgg ttatgcggcg ctggcccccg tgttcgggct gacgccggaa
      121 atcggccttg ccgtctcgct cttgcgccgg gcccgcgaca tcgtcgtggc cgtgccggtc
      181 cttctgatct ggcagctgat cgaaggacgc cgcgctgcgc ggtgaggcgt cgcctctctc
      241 cctgcgctcg taggcagcgt gggaatctga aaacctgaaa tgtccgcttg gggtcaaaag
      301 cggacatggg gctgacctgg acggaatgac cgctttcggc aaaaaaagcg gagattcgga
      361 cgatcccctt ccgggcctat gcgccaaatg cgtgcgcccc gatgggtcga catcgttccc
      421 cgtaaaaatc ataggccgga acgccaagcg cgaaaccgca tggtccccag ttcagcgtca
      481 attgcgtttg actcaatggt gggcgtgaca agcacatagg gtgcacttaa ttgcgatacg
      541 tttcccgcca ttactattgc gtttcgccaa ataaatccgt ccgaatcgag caaacgcacg
      601 ttgggccaca cattcgccat acattccgcg aggcgatctg cgtggtggtc aaaatcatgt
      661 tggacgtgaa catttgcgaa tatcgccccg cgctgagtga gtcgatcgcg cactagtcca
      721 aagaagcgca gggactggag atgcgcgggg atgcggtcac cgtgatacgc atcaagcacc
      781 accgcgtcgt atagatttga atccgatcgc aaatgggatt tcccgtcagc gatatggcat
      841 gcagtatcgt ccggcagctt gaaatattgt tttgcaatga cgaaggacgc gggattgaca
      901 tcaacgattg ttacctttcg tcccgctcgc acaagcatag tagccagcgt gccgccgccg
      961 cagcctatca aaagaatcct gcgggccccg gtctgagaaa taagcccgaa gatcgcatga
     1021 atataggagg cgaggcttat gccgttactg tctgcttcac tttggcagca gccgccctgt
     1081 tcatatccaa tcgcgcccgt cgattttctt ttgaaaaccg tgatcgttcc gaagtctgat
     1141 tcaacggcgt ccaatctaat catcacaaac ctgtccgcag taagccaagg cgtgtcgcag
     1201 gcgtggtagg cagcccctga cgagatggat tacctcctac tgccgtggaa caatgcttac
     1261 cgattaaccc ttgcggcatc gtaaacaaat caccattgaa gcgctcttag cggacattcg
     1321 aggaagggct gatgcggcgg cgctttggcc gccaccgccc catcgtctgt caacagtttg
     1381 ggtt
//
LOCUS       DATCBB010000521         1691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_211506, whole genome shotgun sequence.
ACCESSION   DATCBB010000521 DATCBB010000000
VERSION     DATCBB010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211506"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VII37_05750"
     CDS             complement(<1..288)
                     /locus_tag="VII37_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069266.1"
                     /translation="MIAMATAATAMGVSVLPAERFFRASLLLLIFTSVCTLALTGKLD
                     PFTAVLAPGAVVYKAVRWWRGRPNELQNSRATWLVIAYLGFFPLDMFILSRV"
     gene            complement(435..1559)
                     /gene="mutY"
                     /locus_tag="VII37_05755"
     CDS             complement(435..1559)
                     /gene="mutY"
                     /locus_tag="VII37_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5069267.1"
                     /translation="MSAPSFLKSSELKKFRTELLNWFATYQRDLPWRRDNDPYRIWIS
                     EIMLQQTRVAAVIPYFEKFCARFPNVRALAEAEESEVLRMWSGLGYYSRARNLQAAAQ
                     QIVREHGGTFPATEQHIRALRGIGKYTTNAILSIAFGKKHAVVEGNVARVIARLEALR
                     GDLREGARWESLQHTADRLLSLGAPANWNQAMMELGATICTPRSPQCLLCPVANFCRA
                     RKLGLQDVIPEKRKQRATVDVLLAAAVLVDQRNRCILLPPPENSLEKAQANDIPTLVS
                     KMWHFPTTPVSEDPASDLRKYLKNVLGAKGLPLRFVALPKIRHAVTYRAISLLPFRVE
                     FKKLPRVTGAKMIPLKDFSSLPVSNLTRKVARAALAVLDN"
BASE COUNT          396 a          441 c          501 g          353 t
ORIGIN      
        1 cacgcgagaa aggatgaaca tgtcgagggg aaaaaatccc aggtaggcaa tgaccagcca
       61 ggtcgcgcgg ctattctgga gttcgttggg ccgtcctcgc caccagcgaa cggctttgta
      121 aaccaccgcg ccgggcgcaa gaacagcggt gaaaggatcg agcttgccag tcaaggccag
      181 agtgcacact gacgtaaaaa tcagcagcag caacgaagcg cggaaaaagc gttcggcggg
      241 aagcacgctc acacccatag cggtggcggc agtggccata gcgatcatcg tcgcattcgg
      301 ccgttgggct tcgcttcctt agcgtggtac aggaccaccg cggaagttac tatcgaatct
      361 ccgctcgctg acggatggga agaatctggc ttgaaacggt aaagaaaaga tgtgctcagc
      421 gacggaatca attctcaatt atccagcacc gctagcgctg cacgcgctac tttgcgcgtt
      481 aagttggaaa ccggcaggga ggaaaaatcc tttagtggaa tcatttttgc gcccgtgacg
      541 cgaggcaatt ttttgaactc cacccggaag gggagcaagc tgatggcccg gtaggttacc
      601 gcgtggcgaa tcttaggaag cgcaacgaat cgcaatggca gtccctttgc ccctaagaca
      661 ttttttaaat atttacgcag gtcgctcgcc ggatcttcgc taacgggtgt ggttgggaag
      721 tgccacattt tcgaaaccag cgtgggaata tcgttggcct gcgctttttc caggctgttt
      781 tctggaggag gtaacaaaat acacctgtta cgctgatcga ctaacactgc tgcggcaagc
      841 agcacgtcga cggtcgcgcg ctgcttgcgt ttctctggaa ttacgtcctg gaggccgagt
      901 ttgcgggcgc ggcagaaatt cgccactggg cataacaagc attgcggcga gcgtggtgtg
      961 cagatggtgg cgccaagctc catcatggct tgattccagt tagcgggagc cccgagcgac
     1021 aatagccggt cggcagtgtg ctgcagcgat tcccaccgtg ctccctcacg caagtctccg
     1081 cgaagggctt ccagccttgc gattacacgg gcaacgttgc cttccactac ggcatgtttt
     1141 ttcccgaagg cgatgctcag aattgcgttg gtggtgtact tcccgatacc gcgcaaagct
     1201 cgaatatgct gttccgttgc ggggaaagtg ccgccatgtt cacgaacgat ttgctgggca
     1261 gcggcctgta aattccgcgc gcggctgtag tatcctagcc ccgaccacat gcgaagcact
     1321 tcagattcct cggcttccgc cagagcgcgc acgttgggga agcgcgcgca gaatttctca
     1381 aaatagggaa tcacggcagc aacgcgggtc tgctgcagca taatttcgga aatccagatg
     1441 cggtaagggt cgttgtcgcg gcgccaaggc agatcccgct ggtaagttgc aaaccagtta
     1501 agcaactccg tgcgaaattt cttgagttcg gaagatttga gaaaagaagg cgcgctcatg
     1561 aacgccgtat tctcgcacac tggctgaaat caactgggag aaaggcgaat ctacttcagc
     1621 acatcatcga ggaaaattac gtaggaggaa taccacacgt tggcggggat ggagccgcga
     1681 ggttggctgg t
//
LOCUS       DATCBB010000522         1164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_341349, whole genome shotgun sequence.
ACCESSION   DATCBB010000522 DATCBB010000000
VERSION     DATCBB010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341349"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VII37_05760"
     CDS             complement(<1..772)
                     /locus_tag="VII37_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5069268.1"
                     /translation="MSLASVSRMEAKLLLPTYERQPVLFVRGRGVYLWDSEGKRYLDF
                     LSGIGVNALGHNHPVIQATIKRQAGRLIHISNLFFHEYQAELARLLTEISGLDRAFFC
                     NSGTEAWEGALKLARAFAQTNNKNGSKPKWRILAMNNSFHGRTFGALATTGQPKYRAP
                     FVPLMPGVSFVNFNDVEELRRKFDSSVCAVCIETIQGEGGICPVDPEFLKLASRLTKE
                     SGALLVLDEIQSGLGRTGRHFAYQHYGVLPDIVTVAKPL"
     gene            complement(769..>1164)
                     /locus_tag="VII37_05765"
     CDS             complement(769..>1164)
                     /locus_tag="VII37_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069269.1"
                     /translation="RWLKAIWAEGGIPVLSSVALGGDGQYYNVNADQMAAACAIACEA
                     DALIFLTDVAGVKQADGSVMARLDIKQAAGLIVDSVVSGGMMPKLEACTQALKSGVGR
                     VRILPASEVEVLPLFYFTKIECGTEVLRA"
BASE COUNT          272 a          337 c          310 g          245 t
ORIGIN      
        1 ccagtggctt ggcgacggtc acaatgtcag gcagcacgcc gtagtgctga taggcgaagt
       61 ggcgtccagt gcggccaagt ccgctttgaa tctcgtcgag tacgagcaac gcgccgctct
      121 ctttcgttaa gcgcgaggcc agcttcagaa attctggatc gacgggacat atcccgcctt
      181 ctccctgaat agtttcaatg catacggcac agacactgga atcgaatttg cgtcgtaact
      241 cttcaacatc gttaaaattc acgaagctca cacccggcat gaggggaacg aacggtgccc
      301 ggtatttagg ctgacccgta gtcgcgagcg ctccgaatgt gcgtccgtga aaagaattat
      361 tcatggccag aatgcgccat tttggtttgc tgccattctt gttattggtt tgcgcaaaag
      421 cgcgggcaag tttcaacgct ccctcccacg cctcagttcc gctgttgcaa aagaaggcgc
      481 ggtcgaggcc agagatttca gtcagcaaac gggcgagctc agcttgatac tcgtgaaaga
      541 aaagattcga aatatgaatc aagcgtcctg cctggcgttt gattgtcgcc tgaataacag
      601 gatgattgtg gccaagcgcg ttgactccga ttccgctcag gaaatcgaga tagcgcttgc
      661 cctcggaatc ccagagatac acgccgcgcc cacgcacaaa aaggacaggc tggcgctcat
      721 acgtcggaag caagagcttc gcttccatgc gggaaactga agcgaggctc atgctcttaa
      781 aacctcggtg ccgcattcga tcttggtgaa ataaaatagc ggcaacactt cgacctccga
      841 ggcgggcaaa atacgtacgc gccccactcc gcttttgagt gcttgggtgc aagcctcgag
      901 cttcggcatc atgccgccgc tgactacgga atccacaatg agtcccgctg cctgcttgat
      961 atccaaacga gccatcacgg aaccgtcggc ttgcttcacg ccggccacgt ccgtcaaaaa
     1021 aatcaacgca tccgcctcgc acgcgatggc gcacgcggcc gccatttgat cggcattcac
     1081 gttgtagtac tggccgtcgc ctccaagtgc aaccgaggag agcacgggaa tgccgccttc
     1141 cgcccatatc gctttgagcc agcg
//
LOCUS       DATCBB010000523         1334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_179411, whole genome shotgun sequence.
ACCESSION   DATCBB010000523 DATCBB010000000
VERSION     DATCBB010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179411"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..>1334
                     /locus_tag="VII37_05770"
     CDS             84..>1334
                     /locus_tag="VII37_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY5069270.1"
                     /translation="MTSELHEKQLHETHAVLMELSEDSLLKPFRQMAGQAAPGADLGG
                     WYHYDPDYDSNNVDVGFAPSATFGQWVSALARTYAITGDAATREKVIRLNRLYAKTIS
                     GDFYEKNRFPAYCYDKLVCGLIDSHELVHDPDAFSILEHTTNTALPHLPGKAIEHGER
                     WRQNPAEGYTWDESYTISENLFLAYQRGAGNRYRDLGSQYLDDEHYYDPLADGRNVLA
                     GRHAYSYVNSLCSAMQAYLTLGSAKHLRAARDAFEMLKAQSFATGGWGPDEQLRAPGS
                     GDVAASLTNTHNSFETPCGSYAHFKLTRYLLRVTQDSRYGDSMERVMYNTVLGAKPLL
                     ADGSTFYSSDYNFEGRKIYKNVRWPCCSGTLPQVAADYRINTYFCDSDGMYVNLYIPS
                     TVRWSHEGAQISLTQKGEYPFDGAV"
BASE COUNT          305 a          365 c          365 g          299 t
ORIGIN      
        1 ctgtgacttc ccattttaaa tttccagtag cgctgggcac gtcaccaagc ccactcaaag
       61 aattcggata tgccgacgta actttgacca gtgagctaca cgaaaaacaa ctgcacgaaa
      121 cgcatgcggt attgatggag ctgagcgagg acagtttact gaaaccgttt cgtcagatgg
      181 ccggacaggc ggccccggga gcggatctgg gcggttggta tcattacgat ccggactacg
      241 atagcaacaa cgtggatgtt ggattcgcgc ccagcgccac tttcggccag tgggtctccg
      301 cactggcgcg aacttacgct ataaccggcg atgctgcaac gcgagaaaag gtaatcaggc
      361 tgaatcgcct ctacgccaag accatctcgg gcgattttta tgagaagaat cgcttccccg
      421 cctactgcta cgacaagttg gtgtgcggcc tgatcgattc acatgaactt gtgcatgatc
      481 cagacgcctt ttcgatcctg gagcacacta ccaacaccgc gctcccgcat ttacctggca
      541 aggcgatcga gcatggggaa agatggcgcc agaatcccgc cgagggatat acgtgggacg
      601 agtcctacac aatttctgaa aatttatttt tggcctacca acgtggtgcg ggaaaccggt
      661 atcgcgatct cggttctcag tatttggacg acgagcatta ctacgaccct ttggcggatg
      721 gccgcaacgt ccttgcgggt aggcatgctt acagctatgt gaattcgctg tgctccgcga
      781 tgcaggcgta tttgacgctg ggcagcgcaa aacatctgcg tgccgccagg gacgcttttg
      841 aaatgctgaa agcgcaaagt ttcgcgacag gtggctgggg gccggacgag caacttcgtg
      901 ccccgggcag cggcgatgtt gccgccagtc tgactaatac acataacagc tttgaaactc
      961 cctgtggctc ctacgcgcac tttaagctga cgcgttactt gcttcgggtg actcaagact
     1021 cgcggtatgg cgacagcatg gaacgtgtga tgtacaacac cgtgctgggc gctaaacctt
     1081 tgctggccga tggcagcact ttttattcct ccgactacaa tttcgagggg cgcaagatct
     1141 acaaaaatgt ccgctggccc tgctgctctg ggacgctgcc acaagtcgcc gccgactatc
     1201 gcatcaacac ttacttctgc gattccgatg gcatgtacgt caacttgtac atcccttcca
     1261 ccgtgcgctg gagtcatgaa ggtgcgcaga tctcactgac gcagaaaggc gaatatccat
     1321 ttgatggcgc ggtg
//
LOCUS       DATCBB010000524         1159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_276662, whole genome shotgun sequence.
ACCESSION   DATCBB010000524 DATCBB010000000
VERSION     DATCBB010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276662"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VII37_05775"
     CDS             complement(<1..365)
                     /locus_tag="VII37_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069271.1"
                     /translation="MNFRTTNCALFKAAKIYTFRFSVPIAVALFLCVPGGVPQEQLDS
                     DAVINHLNAVIKLYGNASAELQIGGAPSDVIYQENGKSYTADVVKLAFQAARAEAAAT
                     RATEKNGAPSTSSGQSSAQT"
     gene            complement(366..>1159)
                     /locus_tag="VII37_05780"
     CDS             complement(366..>1159)
                     /locus_tag="VII37_05780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5069272.1"
                     /translation="ELGRKKIKKDSPISWNDSGTLGVVNDVPGGTVDPGKLISGLAQA
                     AQQSGALIFEHARAEKISHEQPLELEVSGKRVRAGQVLIATNAESLELSDLSGGAEPK
                     FTLALATEPLTDAQLAEVGLSTHRPFYTVDMPYLWGRPLHGHQVIFGSGLVHLAHWSE
                     LLNLNISNGEAAGLMRLLESRVHSLHPALENIQFTNRWGGPILIADDMTPLFRHHLKS
                     KNIIVLGGYSGHGVALSVYLGRWAADVLAGKRKLPSWNKSSGAKH"
BASE COUNT          262 a          324 c          305 g          268 t
ORIGIN      
        1 gtctgcgctg agctttggcc agagctggta ctgggcgcgc cgtttttctc tgtggcgcga
       61 gtcgcagcag cttccgctcg ggccgcttga aatgccagct tcacaacatc ggcggtataa
      121 ctctttccat tttcctgata aatgacatcg ctgggggcgc cgccgatttg cagttcagcg
      181 gaggcgttgc catacagttt aatgaccgca ttcagatgat tgattaccgc gtcgctgtcc
      241 aactgctcct gtggcacgcc acctggtaca caaagaaaca aggccacggc aatgggcacg
      301 gaaaatcgaa acgtatagat ttttgctgct ttgaaaagtg cgcaattcgt ggtgcgaaaa
      361 ttcattcagt gtttggctcc cgaggattta ttccacgacg gcaactttct tttaccagcc
      421 aaaacgtcgg ctgcccagcg gcccagataa acggaaagcg cgacgccgtg gccgctgtac
      481 cctcccaaga cgattatgtt tttgcttttc aggtgatgcc ggaacaaggg agtcatatcg
      541 tccgcaatta aaatcggacc gccccaacgg ttggtaaatt gaatattttc gagcgccgga
      601 tgcagggaat gcacacgcga ctccagcagg cgcatcagcc cggcagcctc gccgttcgaa
      661 atattcagat tgagcagttc gctccagtgc gcgaggtgta ccaggccgct gccaaatatc
      721 acttggtggc catgaagagg gcgtccccac agataaggca tgtccacggt ataaaaaggt
      781 ctgtgcgtgg agagaccaac ttctgccagt tgagcgtccg tcaaaggctc cgttgccaac
      841 gccagagtaa atttgggctc cgccccgccg gacaaatcac tgagctccaa agattcggca
      901 ttggtggcga tcaatacctg gcctgcgcgc acacgcttcc ccgatacttc caattcgaga
      961 ggctgctcat gggaaatttt ctccgcacgc gcgtgctcaa aaatgagggc gcctgattgc
     1021 tgcgccgcct gggccagacc actaattaat tttcccggat cgacggtacc tcctggaaca
     1081 tcatttacca cgccaagagt tccggaatca ttccaggaaa tgggggagtc tttttttatt
     1141 ttcttgcggc ccagctccc
//
LOCUS       DATCBB010000525         1910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_179474, whole genome shotgun sequence.
ACCESSION   DATCBB010000525 DATCBB010000000
VERSION     DATCBB010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179474"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VII37_05785"
     CDS             <1..1219
                     /locus_tag="VII37_05785"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069273.1"
                     /translation="NRNGIYVFVNRRLVRDRLILHAIHEAYRNILPAGVFPATLLFLD
                     MPYDEVDVNVHPAKIEVRFRRPQFVHDFTRDTIRQTLTGTRAIASFATASAGAVQSMR
                     MPGAGVGISMAPTTAEFSTPEPGINGGAGPSGIPRAIIPVDDIGMGSGVGSDGAFDLT
                     SAPLRPLAQRLAFGQGGFSTESGLTRGASAGNWAANLAGPNGDAPASLPRPDQISDLK
                     PLGQVSSSFIVAVNGGGLWLVDQHVAHERVLFEQHLSARRAGKIEAQRMLMPMVIELA
                     PRQIVIYEKIAEELAANGFEVEPMGPRSVAIQAVPAGILASDAEKLLTEILDGIERES
                     AAISIETLQAKIAASTACHAAIKVNLPLDQTKMEWLLAELAKTDCPMSCPHGRPVVLR
                     YSVKEIERAFQRI"
     gene            1297..>1910
                     /locus_tag="VII37_05790"
     CDS             1297..>1910
                     /locus_tag="VII37_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5069274.1"
                     /translation="MSDTQKTAVIFGVANKRSIAWAIAQKLQQAGWQLAITYQNERLA
                     QEAKGLIDDLPGATGFMCDVAHDDQIAKLFEELKQKYGTLQGVVHSVAFAPAADMAGE
                     FLNTSREGFRIAHDVSVYSLIAVARAAAPLMSNGGGIVTLTYYGAEKVVPKYNVMGVA
                     KAALEACVRYLANDLGPKNIRVNAISAGPIKTLAARGVSGLGEML"
BASE COUNT          438 a          514 c          577 g          381 t
ORIGIN      
        1 aaatcgcaac ggaatttacg ttttcgtgaa tcgccgcctg gtacgtgatc gcttgatcct
       61 gcacgcgatt catgaagctt accgaaatat cttgcctgcc ggtgttttcc cggccacgct
      121 cttatttttg gatatgccgt atgacgaagt ggatgtgaat gtgcacccgg cgaaaatcga
      181 ggtgcgcttc cgccggccgc agttcgttca cgatttcacg cgcgacacca tccgccagac
      241 gttgacgggc acacgcgcga tcgcgagctt tgcaactgca tccgcaggtg ccgttcagtc
      301 gatgcgaatg ccaggagctg gtgtgggaat ctcaatggcc ccaacgaccg cggagttttc
      361 tacgccagag ccgggaatta acggcggcgc cgggccatca ggaatcccac gcgccataat
      421 tcccgtggac gatatcggta tgggttccgg cgtgggctcg gacggcgctt ttgatttaac
      481 ttccgcgccg ctcaggccgc ttgcgcagcg actggcgttc ggacagggtg ggttctccac
      541 cgaatcggga ctcacgcgag gagcttcagc tggaaattgg gccgcgaacc tcgcgggccc
      601 aaatggcgat gcaccggcca gcttgccgcg gcccgatcag atttccgatt taaaaccgct
      661 cggacaggtg agctccagct ttatcgtggc cgtgaatggt ggcgggctgt ggctcgtcga
      721 tcagcacgtg gcccacgagc gcgtcctatt cgagcaacac ttatcagcgc gccgcgcggg
      781 aaaaatcgaa gcacaacgaa tgctgatgcc catggtgatc gaactggcac cgcgacagat
      841 tgtcatctac gaaaaaatcg ccgaagaatt ggccgccaat ggattcgaag tggaaccaat
      901 ggggccgcga agtgtggcca tccaggccgt cccagcggga atcctcgcca gcgatgccga
      961 aaaattgctc acggaaattc tggatgggat cgaacgagaa agcgcggcca tttcgatcga
     1021 aacactgcag gccaaaatcg cggcgtcgac tgcctgccac gcggccatca aggtaaatct
     1081 gccgctggat caaaccaaaa tggaatggct gctggcggag ctagcgaaaa cggattgtcc
     1141 catgagttgt ccgcatgggc ggccagtggt gttgcgatac tccgtgaagg aaatcgagcg
     1201 cgcgtttcaa agaatataga tccacgtcgc gcgaagacct tcacgtgcgc gaaactgtag
     1261 cagtaaataa atgacgggcc gaggtgaatt cgaagcatga gcgacacgca aaagactgct
     1321 gtgatttttg gagtggccaa caaacgcagc atcgcgtggg ccatcgcgca gaaattgcag
     1381 caggccggct ggcagctggc catcacctac cagaacgagc ggcttgctca ggaagccaaa
     1441 ggactgatcg acgaccttcc gggagctacg ggattcatgt gcgacgtcgc acacgatgac
     1501 cagatcgcga aactatttga agaattgaaa cagaaatacg gcacgctgca gggtgtcgtg
     1561 catagcgtgg ccttcgcgcc tgctgcggat atggccggag aatttctgaa cactagccgg
     1621 gaaggttttc gcattgcgca cgatgtgagc gtttattctc tgatcgccgt tgcgcgcgct
     1681 gcggctccgt tgatgagcaa tggcggcgga attgtaacct tgacctacta cggcgcggaa
     1741 aaagtggtac cgaaatacaa tgtgatgggc gtggccaaag ctgcgctgga agcctgcgtg
     1801 cgttatttgg ccaacgattt ggggccgaaa aacattcgtg ttaacgccat atccgcgggg
     1861 ccgatcaaaa cattggcagc ccgaggagtt tccggtttgg gcgaaatgct
//
LOCUS       DATCBB010000526         1740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_438550, whole genome shotgun sequence.
ACCESSION   DATCBB010000526 DATCBB010000000
VERSION     DATCBB010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438550"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /gene="polA"
                     /locus_tag="VII37_05795"
     CDS             <1..1650
                     /gene="polA"
                     /locus_tag="VII37_05795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY5069275.1"
                     /translation="LPREQPIAVWLNLEPGDRESEGFGTRIASIEVSSKPGEGRAVWM
                     DEKGEALTALRPLLSDPQRPKIVHDPKLFHLLAGRMANVLHATQIYSYLLRPTTANHN
                     FTDVVMRQFNAMIGGGPGERADYLQRLAPALQAQIGAEQIANVYAKIDLPLAPVLADM
                     ERAGIRVDPKALDAMSQVMETEIRRLEKEIWELAGMEFNVNSPTQLAEILFDKLNLQP
                     SQRRGRVKGRSTAVDILEELSAKHPLPRKIIEYREIAKLKSTYVDSLPKLVNRETGRL
                     HTSYSQTGTATGRLSSSDPNLQNIPIRTELGRQIRAAFVADEGKVLLSADYSQIELRV
                     MAHFSRDPVLLEAFNSGEDIHARTAQEVFGVGQMAQTAEHRRAAKVINFGIIYGLSPF
                     GLAQQLNIENREAAQFINAYFARYRGVKQYLDDTLADTRATGVAKTLFGRIRPIPEIN
                     SAQFQLRNFAERTALNSPLQGTAADFIKLAMISIDRRLTEENLQAKMILQVHDELVFE
                     VPKNEIEAVSKLVREEMEGVYQIAVPLLVEVCSGPNWRDLD"
BASE COUNT          405 a          482 c          478 g          375 t
ORIGIN      
        1 ctaccccgcg aacaacccat cgcagtgtgg ctaaacctcg aacccggcga tcgcgagtcc
       61 gaaggtttcg gcacgcgcat cgcttccatc gaagtgtcct ccaaaccggg cgaaggccgg
      121 gcagtatgga tggacgaaaa gggcgaagcg ctcacggcgt tgcggcccct gctgagcgat
      181 ccacaacggc ctaaaattgt ccacgatccg aaattgtttc acttgctcgc gggaagaatg
      241 gccaacgttt tgcacgccac gcagatttat tcctatttgt tgcgtcccac caccgcgaat
      301 cacaatttta ccgacgtggt catgcggcaa ttcaacgcca tgataggcgg cgggcccgga
      361 gagcgcgctg attatctgca gcggctcgct ccagccttgc aggcgcagat tggcgccgaa
      421 cagatcgcca acgtatacgc aaagatcgat ctacctctcg cgccggtgtt ggccgacatg
      481 gagcgcgccg gtattcgcgt cgatcccaaa gcccttgatg ccatgtctca ggtaatggaa
      541 acagaaatac gtcggctgga aaaggaaatt tgggaacttg ccggcatgga attcaatgta
      601 aattcgccga cccagctggc ggagatttta ttcgacaagt tgaacctgca gccctcgcag
      661 cgtcgaggac gggtcaaggg ccgttcgacg gcggtggata ttctggaaga attatcggcc
      721 aagcatccgt tgccccgaaa aattatcgag taccgggaaa ttgccaagct caaatcgacg
      781 tatgtcgatt ctttaccaaa attggtgaat cgcgaaactg gcaggctgca caccagttat
      841 tcgcagacgg gcacggccac gggacgactg agctcttccg atcccaacct gcaaaatatt
      901 ccgattcgaa cggaattggg acgccagatt cgtgccgcgt ttgtggccga cgaaggcaag
      961 gttttgctat ccgccgacta ctcgcaaatc gagctccgcg tcatggcgca cttttcgcgt
     1021 gaccccgttt tgctggaagc attcaatagt ggcgaagata ttcacgcacg caccgctcaa
     1081 gaagtttttg gcgtgggcca aatggcgcag accgcggaac accgccgcgc cgccaaggtc
     1141 atcaatttcg gaattattta cgggctttcg ccgttcggtc tcgcgcagca attgaatatc
     1201 gagaaccgcg aggctgcgca atttatcaat gcctacttcg cgcgatatcg cggcgttaaa
     1261 caatatctcg acgacacgtt ggccgatact cgcgcgactg gagtggctaa aaccctgttt
     1321 ggcagaattc ggccgatccc tgaaattaat tccgcgcaat ttcaactccg taatttcgcg
     1381 gagcgcaccg cgctcaattc gccgctgcaa ggcaccgccg cggactttat caagctggcc
     1441 atgatcagta ttgaccgccg tctgaccgaa gagaatctgc aagccaaaat gattctgcag
     1501 gtgcacgacg aactggtttt cgaggtaccg aaaaacgaaa tagaggccgt ctcgaaattg
     1561 gttcgagagg agatggaagg cgtctaccaa atcgccgttc cgctgctggt tgaggtgtgt
     1621 tcaggaccga attggcgtga cttggactaa ggttctggcc ctaccggggc atgttcgcgt
     1681 gcgcaacctt ttaccgcgct cagggttctt aataacggtg gcgtgtgcgt gcatactttc
//
LOCUS       DATCBB010000527         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_276677, whole genome shotgun sequence.
ACCESSION   DATCBB010000527 DATCBB010000000
VERSION     DATCBB010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276677"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VII37_05800"
     CDS             <1..996
                     /locus_tag="VII37_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069276.1"
                     /translation="RAQMGDVAFKSLLDDFYTKYEGKSARIDDFAAMAQQHAQASVRK
                     GEAPPELRGFFTQWLNSTGIPEFAVDYVVYRTPKGFRISGKIKQPLDTFRMPVELRID
                     TEGNPELKTIDVIGSESDFTVETFGRPKPGGIRVDPNNLILKASTNLRARAAVARGEE
                     LAEQGRYYDAVTQYQRALAIQPNRPLANFRMGEAFFYQKNYQASANAFREALQTVPEP
                     AEKWTEVWSHIYLGKIFDLLAQRERAVNEYSKARQTNDNTGGAQQVAEGFLKKSYTEG
                     EKTAVAPGTGGTATPAAPAAIPPPSEGNRPVLKKRTDPAQFLPDYSNKTASDFHP"
     gene            1090..1539
                     /locus_tag="VII37_05805"
     CDS             1090..1539
                     /locus_tag="VII37_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069277.1"
                     /translation="MAHIYMQFDFADEENAQQARHKLDVWKQAFRLDKKLQYKLERPE
                     EADAENEPGSGDAAADSKDAEKPKSKSKSKHAKKAGRADGDSEGNGTGSVSLLVRLYF
                     SSHEKLSEQRWLDRIPTEEPFKSASPKVLRQGEDGFEEALKQFSDLD"
     gene            1678..>3072
                     /locus_tag="VII37_05810"
     CDS             1678..>3072
                     /locus_tag="VII37_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5069278.1"
                     /translation="MASQTRIKVAKLDAPKMHGLDRDTLVRLYRTMYLSRKLDDREIQ
                     LKRQNKIYFQISSAGHEAVTAAAGLLLRPGIDWIYPYYRDRALCLMLGVTPLETLLQA
                     VGAKDDPSSGGRQMPSHWGHPEWNIVSRSSCTGTQFVQAVGAAEATVYYQERPKALAK
                     ARKAPLGQYVSYEDNEIVYVSSGDGATSEGEFWESLNTACIKKLPMFYLIEDNGYAIS
                     VPVEVQTAGGSISKLVGSFPGLQVAECDGTDPLESYVACKEAIQYCRERRGPALVHAQ
                     VTRPYSHSLSDDEKLYKSAEEREKEAHRDPLSKFSLFLVREGLLDQKQIEDLEAQVDN
                     EVREAADQALAAAEPSIDSIKVNVYSPDVDPSSSTFEAQPQYHGAPKTMVEMVAATLS
                     DEMLRDERITVFGEDVADASREENLKSVKGKGGVFKATAGLQRKFGPDRVFNSPLAEA
                     SIVGRAIGMATRGLK"
BASE COUNT          765 a          839 c          845 g          623 t
ORIGIN      
        1 cgggcgcaga tgggtgacgt ggccttcaaa tccctgctgg acgattttta taccaagtac
       61 gaaggtaaga gcgcgcgcat cgatgatttt gccgccatgg cgcaacagca tgcgcaagcg
      121 tcagtcagga aaggcgaagc gccgccggaa cttcgcggat tttttacgca atggctgaat
      181 tccacgggca taccggaatt tgcggtggat tacgtggtgt atcgcacgcc caaaggattt
      241 cgtatttccg gcaagatcaa acagcccttg gataccttcc gcatgccggt ggaattgcgt
      301 atcgatacgg aaggcaatcc tgaattgaaa acgattgatg tgattggttc ggagtccgat
      361 tttaccgtcg agacgttcgg tcgcccgaaa cccggcggaa ttcgcgtcga tcccaataac
      421 ctgattctca aggccagtac aaatttgcgc gcccgggcgg cggtggcgcg tggcgaagaa
      481 ttggcagagc aggggcgtta ctatgatgcc gtcacgcagt accagcgtgc tttggctatc
      541 cagccaaatc gtccgctagc caatttcaga atgggtgagg cattttttta ccagaagaat
      601 tatcaggctt cagccaacgc gtttcgcgag gccttgcaga ccgtgccgga acccgctgag
      661 aagtggacgg aagtttggag ccacatttat ctaggaaaga tttttgattt actggcgcag
      721 cgggaacgcg cggtaaacga gtacagcaaa gcaaggcaga ccaacgacaa tactggcgga
      781 gcgcaacaag tcgctgaagg atttttgaaa aagtcttaca cggaaggtga gaagacagcg
      841 gtggcaccgg gcacgggggg cactgcgact cccgcggccc cagcggctat cccaccgccg
      901 tctgaaggaa atcgccctgt gttgaaaaaa cgcaccgacc ctgctcaatt tttgcccgac
      961 tactcgaaca aaacagcatc cgattttcat ccttaaataa gtaagaatcg ccgtacgcat
     1021 cgtttgaatc tgcgcgcggc gcggccctct ccttgacttg ccgcgtaacg cgtcgcaaac
     1081 tttgggccta tggcccacat ctacatgcag tttgattttg ctgacgagga aaacgcacag
     1141 caagcgcgcc acaagctgga cgtctggaag caagcgtttc gcctggataa aaagcttcaa
     1201 tacaaattag aacgcccgga agaagctgac gccgagaatg agccgggaag cggcgacgct
     1261 gcagccgact caaaagacgc ggaaaaaccc aagtccaaat cgaaatcgaa acacgctaag
     1321 aaagccgggc gcgccgacgg cgactctgaa ggcaacggca ctggcagcgt atccctgcta
     1381 gtacggctgt atttctccag ccacgaaaaa ttatccgaac aacgctggct tgaccgtata
     1441 cccacagaag agccgttcaa gagtgcctcc cccaaagtgc ttcgccaggg agaagatgga
     1501 ttcgaggaag cgttgaagca attcagcgac ctggattaac cgcctaaatc gatgtggctc
     1561 aagaaccttt agtaactcgg ctatgggcta cttttcgcac cagataagaa gcacaaaatt
     1621 ccgctataat taagggtccg caagagacag cttccatcct tcacgaggtc ggcacgaatg
     1681 gcgtcacaga cgcgcatcaa agttgccaag cttgatgccc cgaaaatgca cggcctggat
     1741 cgcgataccc ttgttcgtct gtaccgcacc atgtaccttt cgcggaaact ggacgaccgc
     1801 gagattcagc tcaagcgcca gaacaagatt tatttccaga tctccagcgc cgggcacgaa
     1861 gctgtaaccg ccgcagcagg gctgcttctc cggcccggca tcgactggat ctatccttat
     1921 tatcgcgatc gcgcactttg cctgatgctg ggcgttacgc cgcttgaaac gttgctgcag
     1981 gctgtcggcg caaaagacga ccccagttcc ggcgggcgcc agatgccctc tcactggggg
     2041 cacccggaat ggaacatcgt cagccgctcg tcctgcacag gcacccaatt tgtacaggcc
     2101 gtcggcgctg cggaagctac cgtctattac caggaacgtc cgaaggccct cgcgaaagcg
     2161 agaaaggctc cgcttggcca atacgtcagt tatgaagaca acgaaatcgt atatgtgtct
     2221 tcaggcgatg gggctaccag cgagggcgaa ttctgggagt cgctgaacac tgcatgcatt
     2281 aaaaagctgc cgatgtttta tttgatcgaa gacaacggtt acgccatttc cgtccccgtg
     2341 gaagtgcaaa ccgctggcgg aagcatttcc aagttagtgg gtagttttcc cggccttcag
     2401 gttgcggaat gcgatggcac cgatccgctg gaaagttacg ttgcgtgtaa ggaagcgatt
     2461 caatactgcc gcgagcggcg cggtccagct ttggttcacg cgcaagttac gcgaccatat
     2521 tcgcactcgc tgtctgacga tgaaaagctt tacaaatctg ccgaggagcg cgaaaaagaa
     2581 gcgcatcggg atccgctctc aaaattttcg ctttttcttg ttcgcgaggg actactcgat
     2641 caaaagcaaa ttgaggatct ggaagcgcaa gtcgacaatg aagttcgtga agccgcggat
     2701 caggctctgg ccgctgcgga accctccatc gactcgataa aggtaaatgt ctactccccg
     2761 gatgtcgatc ccagttcttc cactttcgaa gcgcagcccc aatatcacgg cgcgccaaag
     2821 accatggtgg agatggttgc cgccacgctt tccgatgaaa tgttgcgcga cgagcgcata
     2881 accgtatttg gcgaagacgt tgccgacgcc agccgcgaag aaaatctaaa aagtgtcaaa
     2941 ggcaagggcg gggtcttcaa agcgaccgcc ggtctacagc gcaaatttgg ccctgaccgt
     3001 gttttcaatt caccgctcgc ggaagccagc atcgttggcc gcgccatcgg catggccacg
     3061 cgcgggctca ag
//
LOCUS       DATCBB010000528         1319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_50253, whole genome shotgun sequence.
ACCESSION   DATCBB010000528 DATCBB010000000
VERSION     DATCBB010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50253"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VII37_05815"
     CDS             complement(<1..1035)
                     /locus_tag="VII37_05815"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY5069279.1"
                     /translation="MSVDAFQAGIFLLVVAALVKPVGAYLERGFQRQKTCLDPLLVPV
                     ERLIHRILGIDARGEMDWKRYSLAFGIFSAVNVVALYFLLRWQAWLPWYFPKVMTTPM
                     APDLAANTAVSFVTTTTWQAYAGENTLSYFSQILLAGQNFVAGAAGLAVGIAFIRGYV
                     REKTENLGNFWVDLIRALLWVLVPLSVVGSIFLVWQGVPMNFLPYAVAHTIEGGTQTI
                     ARGPVAALEFIKNLGTNGGGFFNVNGAHPFENPTPLTNFVELLAIVVLPAAFTHTFGR
                     MVGKPRDGWVIFWTMTALFIAGLVVCEWAEGSMHPALAHEIAGGNMEGKEVRFGVGGS
                     ALAAVVTSNGA"
BASE COUNT          311 a          392 c          366 g          250 t
ORIGIN      
        1 cgcgccattg gaagtcacga ccgccgcgag ggccgagccg ccgacgccaa agcgcacctc
       61 tttgccttcc atgttgccgc cggcgatctc gtgtgccagc gcagggtgca tgcttccttc
      121 cgcccattcg cacacaacta atcccgcgat aaagagcgcg gtcatcgtcc aaaagatcac
      181 ccagccgtca cgcggtttgc ccaccattcg gccgaaggtg tgcgtaaaag cggcgggcag
      241 caccacaatg gcgagcagtt cgacaaagtt cgtcagcggc gtcggatttt caaaaggatg
      301 cgcgccattg acattgaaaa agccgccgcc gttggtgccg aggttcttga taaactccag
      361 cgcggcgaca ggcccgcgag cgatggtctg agtcccgccc tcgatggtgt gcgcgacagc
      421 gtatggcagg aaattcatcg gcacaccttg ccagaccagg aagatgctcc caacgaccga
      481 gagcggcacg agcacccata agagcgcgcg aatgagatca acccagaaat tacccaggtt
      541 ctccgtcttc tcgcgcacgt aaccgcgaat aaaagcaatg cccacggcaa gtcctgccgc
      601 gccggcgaca aagttctgcc cggcgagcaa tatctgactg aagtagctca acgtgttctc
      661 gccggcgtag gcctgccacg tggttgtggt cacgaaactg acagccgtgt tcgccgccag
      721 atccggagcc attggcgtgg tcattacctt cggaaaatac caaggcaacc atgcctgcca
      781 gcgcagcagg aaatagagcg cgacaacatt gacggcactg aaaatcccga aggccagcga
      841 gtaacgtttc caatccattt cacctcgcgc gtcgatgccc agaatccggt gaatgaggcg
      901 ctccactggc acaagcagcg gatcgagaca tgttttttgc cgctggaaac cccgttccag
      961 atacgcgccg accggcttga ccagcgcagc gacgaccagc agaaaaatgc cagcctgaaa
     1021 agcgtcgaca ctcatgctgg aaactcttgc gagacggtca gagatcctga gtcataaatc
     1081 aagctgatca tcgcgccggt cgtgacagag tttggaattt tggaagagag gagcaatctt
     1141 ctaatcatcc cgtagccgat gaaagttgac gcgctaacac tatttggact cgcggccgtc
     1201 acgagcatga tgatctgcta cgcgttagaa gagcgaagcc gttggtttat tctaggcttc
     1261 gctctcgcat gcgtttttgg ttcgacgtac ggatttttgc aaggcgcctg gccattcgg
//
LOCUS       DATCBB010000529         1683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_309377, whole genome shotgun sequence.
ACCESSION   DATCBB010000529 DATCBB010000000
VERSION     DATCBB010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309377"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(558..809)
                     /locus_tag="VII37_05820"
     CDS             complement(558..809)
                     /locus_tag="VII37_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069280.1"
                     /translation="MADFAKAIEVKGRNMDKFEEIGQRLDKELARLRKYVEEEVAPGT
                     EKRTARFLREVSELLTDAARKLEARNTARETPAAKDTHS"
     gene            864..>1683
                     /locus_tag="VII37_05825"
     CDS             864..>1683
                     /locus_tag="VII37_05825"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5069281.1"
                     /translation="MALPYQWQMRVERWRNAMSGLFGGRNEPPRPKICPSCGTLVGIN
                     ATRCHQCGTNLNFSMAAINRRFSGFFGGRAPITTALLITNILIFGVSVVATLQTGQGG
                     GLRILWGLDGETLYRLGALWTPAIEHGEPYRLVTAMFLHGGLIHIGFNMMVLMDIGPV
                     VEEVYGSARFLFLYTAAGIAGYVLSAFTPFRPHPALGVGASGAILGLIGVMIAITTRR
                     GGSSMQALRSRLISWVVSIFVFGFLMSGIDNWAHFGGLAAGFGLGKVFADREPMN"
BASE COUNT          291 a          439 c          522 g          431 t
ORIGIN      
        1 agatcgatga tgcggttatt cacgaacgga ccgcggtcgg cgatgcgcac cacggtggat
       61 tttccatttg tctcgttggt gacccgcacc atggtttcga acggcagcgt gcggtgagct
      121 gctgtgagtt tattcatgtc ataaatttcg ccgttggagg cctgccggcc gtggaagggc
      181 acgccgtacc acgatgcttc tccttgttcc gtgtagccgg cgcggaccgg aacggagttg
      241 tccctgcggc gctttgtggc ggccccagaa cgcggcgggc tgggtgggct ggagttcctg
      301 gcggattctg acgtgtcttc aggcgtggag gatggttcag gtttgctggc tgccagcggt
      361 ctgcgtgacg cacatcctga gacaacgcca agaacggagg ctaccaaaac tattacaagt
      421 gcgaatttga aagcggcgat ttgtaaagta ctgcgtgcag caggaatttt tcgtggattg
      481 tattgttggg cgaactgttg tcggcgtgtc gctgcgttca ctggcggccc atagatgcgg
      541 ttctcgttcc ggtcctgtta agaatgagtg tccttcgcag ctggggtctc acgagcagta
      601 ttgcgtgctt ccaactttct cgctgcgtcg gtcaacaatt ccgaaacctc gcgaagaaat
      661 cttgcagtgc gcttctccgt gccgggcgca acttcttctt ccacgtattt gcgcaaacgg
      721 gccaattctt tgtcgagccg ttgtccaatt tcctcaaatt tgtccatgtt tcgccctttt
      781 acttcaattg ctttcgcaaa gtcagccaag ttcgctgact tgggtattat aggaacgcgg
      841 tgtgcgggtc gcaaggagga gaattggcgc tcccctatca gtggcagatg cgggtagaac
      901 gctggcgaaa tgccatgagt gggttgtttg gtgggcgcaa tgaaccgccc cggcccaaaa
      961 tttgcccctc gtgcggaaca ctggtgggca tcaacgcgac gcgttgccac cagtgcggca
     1021 ccaatcttaa tttttcgatg gcggcgataa accgacgctt ctccggattt ttcggagggc
     1081 gcgcgccaat caccacggct ctgctaatca ctaacatttt aatttttggc gtcagcgtgg
     1141 ttgccacgct gcagactggc caaggcggcg ggttgcgcat tctgtggggg ctcgatggag
     1201 agactcttta ccgactgggc gccctctgga caccggcgat cgagcatggc gaaccgtacc
     1261 gcctagtgac ggccatgttc cttcacggcg gtttaattca catcgggttc aacatgatgg
     1321 tgctgatgga tatcggaccg gtagtggagg aggtgtacgg ctctgcgcgc tttctgtttc
     1381 tgtacaccgc cgcgggaatt gccgggtatg ttctgagcgc ttttactccg tttcgcccgc
     1441 atcccgcgct gggcgtgggg gcaagcggcg cgatcctggg attgatcggg gtgatgatcg
     1501 ccatcaccac tcgccgcggc ggatcgtcca tgcaagcgct gcgttcgcgc ctgatttcct
     1561 gggtggtctc tatcttcgtt tttggttttc tcatgtccgg catcgataat tgggcgcact
     1621 ttgggggatt ggcggcgggc tttgggctgg ggaaagtttt tgccgatcgc gaaccgatga
     1681 atc
//
LOCUS       DATCBB010000530         1099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_309379, whole genome shotgun sequence.
ACCESSION   DATCBB010000530 DATCBB010000000
VERSION     DATCBB010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309379"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..838)
                     /locus_tag="VII37_05830"
     CDS             complement(227..838)
                     /locus_tag="VII37_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5069282.1"
                     /translation="MTEITTMFWDIGGVILSNGWDRAARKEAAEVFGLDWEEFQDRHD
                     LCFPALDAGLITLDEYLERALFHHPRSFTREEFIAHMFAQSREFPESRAILDEVTRTK
                     KYFIASINNEPLELNNYRIEKFNLRRNFEVFFSSCYVRSRKPEETIFRMALQISQRPP
                     EECLFIDDRPLNLEVPRRQGMNVIHYQDAERLRSELRKYRVEV"
     gene            complement(894..>1099)
                     /locus_tag="VII37_05835"
     CDS             complement(894..>1099)
                     /locus_tag="VII37_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069283.1"
                     /translation="LGIPLVVENFRGKLHRELAFALTEWAALEDARKSTIRLHLSQGP
                     EKGLKQFSDVVIQAMAQIRGGAG"
BASE COUNT          242 a          313 c          305 g          239 t
ORIGIN      
        1 cgaaaagccg actatggcat aacgggaacc cagcgattga tgcaccgcga ggtcaaacat
       61 cgcggggatt accttgcgtt tggcaaggtc gccggaggcc ccgaaaagaa ccaccgcgca
      121 tggttcggtg gttccgtcat aactccccgc aggtttactg ttgtccgccg ccatgacttc
      181 ctctcagaca aaaagctcgc attgaaccga tttaagctcc ccgaaactat acctcaaccc
      241 ggtactttcg cagctcggaa cgcaagcgct cggcatcctg atagtgaata acattcatgc
      301 cctgccgccg cggaacttcc aggttcagcg ggcggtcgtc gatgaacaga cattcttcgg
      361 gcggccgctg cgaaatttgc agggccatac ggaagatggt ttcttccggc ttgcgcgagc
      421 gcacgtagca ggagctgaag aacacttcaa aattcctgcg caggttgaat ttttcgatgc
      481 ggtaattgtt cagttccaac ggttcgttgt tgatcgaagc gatgaaatat ttcttcgtgc
      541 gcgtcacttc gtcgaggatc gcccgagatt ccggaaactc gcgggattgc gcaaacatgt
      601 gcgcgatgaa ttcttcacgc gtgaacgaac gaggatggtg aaagagcgcg cgctcgagat
      661 attcgtccag ggtgatgagg ccggcatcca gcgccggaaa acaaaggtcg tggcggtcct
      721 ggaattcttc ccaatccagg ccgaaaactt ccgccgcttc cttgcgcgcg gcgcggtccc
      781 agccgttgga gaggatcact ccgcctatgt cccagaacat ggtcgtaatt tcagtcaaga
      841 atgcggcctc caggtttacg ccttagccgc gggaatgaac tggcgaggtg tgcttagcct
      901 gcgcctccgc gaatttgggc catcgcttgt ataacaacat ccgagaattg cttcaaacct
      961 ttttccggcc cttgcgacaa atgcagccgg atagtggact tgcgggcgtc ttccagcgcc
     1021 gcccattccg tcagtgcaaa cgccagttcc cggtggagtt tgccgcggaa attttcaacc
     1081 accagcggga tgcccagcg
//
LOCUS       DATCBB010000531         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_407251, whole genome shotgun sequence.
ACCESSION   DATCBB010000531 DATCBB010000000
VERSION     DATCBB010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407251"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VII37_05840"
     CDS             complement(<1..596)
                     /locus_tag="VII37_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5069284.1"
                     /translation="MKTEELNAQPVAANWLSYNGDYTGQRFSGLEQINQTNVSQLRAQ
                     WVFHAPNSRDMEVTPVVVNGLMLVTVSNDVFALDAQTGRTVWHYSRPVSEGLIDDAAQ
                     HHSRGVGVWKSRIYAETDNAHLMCLDSRSGNLLWDVAYAEGNKNYGATSAPLVVKDRV
                     IVGTSGGDDGVRGFVAAYDAASGKLAWRFWTIPGPGEPG"
     gene            complement(865..1563)
                     /locus_tag="VII37_05845"
     CDS             complement(865..1563)
                     /locus_tag="VII37_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY5069285.1"
                     /translation="MSYLNTHAPGAENPANDGAVALLDGFRHVEVASVSDALEKISGH
                     KMYTSHRMRPIFGSKFAGFALTVQLKKEPNNDPHALDGMLAAIDQGGANSVYIMAVED
                     GIDIAGMGGLMGTAMSARGFSGAVIDGGVRDVAYLTKIGFPVYALGIVPSTSVSHYRF
                     AGANVPVICDGVSVNAGDIIVADADGVAVVPRAQAPQVLSLAQEMDYKEHSMYADIEK
                     LKSIQEAVKKFGRL"
BASE COUNT          499 a          555 c          665 g          481 t
ORIGIN      
        1 ccgggttcgc cgggaccggg aatggtccag aaacgccacg ccagtttgcc gcttgcggcg
       61 tcgtaagcgg ccacgaaacc acgaacgcca tcgtcgcccc cagaagtgcc gacgataact
      121 ctatctttga cgaccagcgg ggcactggtc gcgccgtaat ttttatttcc ttcggcgtag
      181 gcgacgtccc aaagcagatt tccagagcgg gaatcgaggc acatgagatg ggcgttgtcg
      241 gtttcggcgt agatgcggga tttccaaacg ccaacgccac ggctgtgatg ttgcgcggcg
      301 tcatcgatga ggccttcgct gacaggccgc gagtagtgcc acacggtgcg accggtttgc
      361 gcatcaagtg cgaaaacatc gttggagacg gttaccagca tcaggccgtt tactactacc
      421 ggggttactt ccatgtcgcg tgaattgggg gcatgaaaaa cccactgggc gcgcagttgc
      481 gagacattag tctggttgat ttgttcgagg ccgctgaatc gttggccggt gtagtcgcca
      541 ttgtaggaga gccaattggc tgctaccggt tgcgcgttca gttcttctgt tttcacgtcg
      601 atcgcaatct ccgagtgagg cgcttgctga gcaccaagag gtgtgaggca aagaactaag
      661 taagccaagg ctctgagtgt ttggcaggac agtacattcg ggcgtctcag ggcggatgaa
      721 attgcgaatt ttttacgagc gtcgccgccg ccacggtgtt cggcaatcac agggatggaa
      781 acacgcgcgt gtgaagagat tttcgtgagc tcatagatca ggttgggcat tcagcaaatc
      841 attcctaatt catggagcgc taaattacaa ccgtccaaat tttttcaccg cttcctgaat
      901 ggatttaagt ttttcaatat cggcatacat ggagtgttct ttgtaatcca tttcttgcgc
      961 taatgacagg acctggggag cttgcgcgcg cgggaccacg gccacgccat ccgcatcggc
     1021 gacgatgatg tcccctgcat tgacggagac tccgtcacag atcaccggca cattggcgcc
     1081 ggcaaaacgg taatggctta cggacgtgga gggaacaatg cccaacgcgt agaccggaaa
     1141 acctattttt gttaggtacg caacgtctcg gacgccgcca tcgatgactg cgccggaaaa
     1201 accgcgcgcg gacatcgcgg tgcccattaa tccccccatg ccggcgatat cgatgccatc
     1261 ttccacggcc ataatgtaaa cggagtttgc gccaccctga tcgatggcgg cgagcatgcc
     1321 atccaatgcg tgaggatcgt tattaggctc ttttttcaac tggactgtga gcgcgaagcc
     1381 ggcgaatttg cttccaaaaa ttgggcgcat gcggtgggac gtgtacattt tgtgcccgga
     1441 aattttctcc agggcgtcgg agaccgaggc cacttccacg tggcggaagc cgtcgagcag
     1501 ggctacggcg ccatcattgg ccggattctc tgcaccgggg gcgtgcgtgt tgaggtagct
     1561 catcgcggtt ctgccggaca gtccggcggc aatggcaaag ggcaggaggc acacgagaag
     1621 gaaagcaaaa agccgtctga atggtgcgaa accggagcgc tgcttcacag aaacctccaa
     1681 tgaacgatcg ccacggtacg gcggcggtaa aatgcgaact gacgatcagt gttaagtatt
     1741 cccgtatcta aatcgattca aacccagaaa gcaattgaca cgaacaaacg tcatgaagta
     1801 ataccacaca cactggttag agtgaatttt tattgtcgat tttttttggc acttatcgct
     1861 cataagatga cgtcgaggtt tgaaaccgct cgtgacaaaa ataatttctc ctaggtggct
     1921 ttcgccgaga tgtgcgcgac ttgtgggtgg tttgctattc atgagcgcgt gctgggcggt
     1981 tgggtcttgg tcatggagcg gcgaacaagc tgtcgtatac gcgcagaaaa gcaagcaggc
     2041 gactagcacg gggccgaggc ataagcagga ttcggaggca gggcgcgagc tgtttgagtc
     2101 tagctgcgcg ggatgtcacg ggctggacgg gcgaggcggc gaaaaggggc cgaatatcgc
     2161 gacacgggcg gagatctcac ggcgttcgga tggcgagatt
//
LOCUS       DATCBB010000532         1117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_341666, whole genome shotgun sequence.
ACCESSION   DATCBB010000532 DATCBB010000000
VERSION     DATCBB010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341666"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1117)
                     /locus_tag="VII37_05850"
     CDS             complement(<1..>1117)
                     /locus_tag="VII37_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5069286.1"
                     /translation="ASGGPTSQSPAVTISSPSPIGDTTALAKEAQGWLIDLIRTNTTN
                     PPGNEQATAKYLAHILEKEGIQSEMLDVAPGRSALVARLRSSAMADPSKALLLVAHMD
                     VVGVDRSKWTVDPFAAMIKDGYLYGRGSIDDKGMLAANLAAFIALKRSNARLNRDVIF
                     LATADEEQGGDASIKILISKYWDKFAAGYALNEGGEVFLKNGRVQYVAVQASEKVAMN
                     VAVVARGTSAHASRPTKDNAVVHLSAAVAKIGGYSAPAHFTTIVRRYFEQLATIEPDE
                     LGKWMRVLDSEDRGEHAQKVVSEASPLWNSMMRDTVAPTMLSAGVRNNVIPAEARANL
                     NIRLIPGNTIDVLLAALTKLVSDPQIKFEVQPDAGLAA"
BASE COUNT          221 a          315 c          330 g          251 t
ORIGIN      
        1 gccgcgagtc cggcatcggg ttgaacttca aattttattt gcggatcgct gaccagcttg
       61 gtcaacgccg ccagcaacac atcgatggtg ttaccgggaa ttagtcgaat atttaaatta
      121 gcgcgcgctt cggcgggaat cacattgttg cgcacgccag cagaaagcat cgtgggagcc
      181 acggtatcgc gcatcatgga attccagagc gggctcgcct cggatacgac tttctgcgcg
      241 tgttcgccgc ggtcctcgga gtccagcacg cgcatccatt tgcccagttc atcgggctca
      301 atggtcgcaa gctgctcaaa atatctgcgc acgatggtgg tgaaatgcgc aggcgcagaa
      361 tagccaccga tcttcgcgac cgccgccgac aggtgcacga cggcgttgtc ttttgtaggc
      421 cgcgaggcgt gcgccgaggt tccacgcgcc accaccgcga cgttcatggc aactttttcg
      481 ctggcctgaa cggccacgta ttgcacgcgg ccatttttga gaaaaacttc tccgccttcg
      541 ttcaacgcat agccggcggc aaatttatcc caatacttgg agatcaaaat tttgatgctg
      601 gcgtcgccgc cttgctcttc atcggcggtc gcaagaaaaa taacgtcacg attcaaacgc
      661 gcgttggaac gtttcagcgc aatgaatgcc gcaagattcg cagccagcat ccctttgtca
      721 tcgatggatc cgcggccgta caaatagcca tctttgatca ttgcggcgaa tggatcgacg
      781 gtccacttgg agcggtcgac gccaactaca tccatgtgtg cgaccagcaa taacgctttg
      841 gaaggatcgg ccatcgcgga actacgcaag cgggccacca gggcgctgcg gccgggcgcc
      901 acgtccagca tctcggactg gattccttcc ttctccagaa tatgtgcgag gtattttgcc
      961 gtggcctgct cgttgcctgg cgggttggtg gtgttggtgc ggatcagatc gatcagccag
     1021 ccctgggctt ctttggcgag cgcggtggtg tcgccgatgg gggatggact gctgatagtt
     1081 acggctggag attgggatgt gggcccgccg ctggctt
//
LOCUS       DATCBB010000533         1134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_407320, whole genome shotgun sequence.
ACCESSION   DATCBB010000533 DATCBB010000000
VERSION     DATCBB010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407320"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VII37_05855"
     CDS             <1..770
                     /locus_tag="VII37_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069287.1"
                     /translation="ADGGVRALPTNIVATLFAVALTAALVAILVLRAVRSRNIGLHPP
                     SDTYISPGPNSKTYHVRATYLIAAVLAGGLLAVCAIACAWFFGTHVETPSAAASLGAA
                     AALAILATGGVVFALDSVRSKLLLHTNALEIRDLFRVRWIPRDAIASRRILRDRYSRS
                     LLLALKAPPDREIKLPMVWATDTEFDAWFAGIPDVDVVAIQEFEDSVKNDTALGNTPE
                     ERSGRLSKARRIAQWSRYAGIGAYSGQLCIQGRSNCS"
     gene            908..>1134
                     /locus_tag="VII37_05860"
     CDS             908..>1134
                     /locus_tag="VII37_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069288.1"
                     /translation="MALGLRGHSDVHVLEWQRTIVCAAVGAAVLTAALVRIRPSMFEN
                     TAKWVLLAILLAPYGYGVGVIANAHWDRSVPT"
BASE COUNT          201 a          314 c          340 g          279 t
ORIGIN      
        1 tcgccgacgg tggagttcgc gcattgccga ccaatatagt agcgacctta ttcgccgtag
       61 cattgaccgc cgcgctagtt gcgatccttg tgctgcgcgc ggtccgttca agaaacatcg
      121 gcctgcaccc gccttcggac acctatatca gcccggggcc gaactccaag acttatcacg
      181 tccgagctac gtatctgatt gccgctgtac tggctggcgg gttattggca gtttgcgcta
      241 ttgcgtgcgc gtggttcttc ggcacgcatg ttgagactcc aagcgctgct gcaagtctcg
      301 gcgcggccgc agctctagcg atcttggcaa cgggcggtgt ggtatttgct ctggattcag
      361 tgcgctcgaa gcttttgctg cataccaatg cgctagagat tcgcgatctg ttccgcgtcc
      421 ggtggattcc tcgggatgcc attgcttcac ggcgcattct gcgtgacagg tactcaagga
      481 gtttgttgct ggcgcttaaa gcgccccccg accgcgaaat caagcttcct atggtatggg
      541 cgacggacac cgagtttgac gcctggttcg ccggaattcc agatgttgat gtcgtcgcaa
      601 tccaggaatt cgaagactcg gtgaaaaacg atactgcact agggaatacg ccggaggagc
      661 gttctggccg tctttccaag gcacggcgca tcgcgcagtg gtcgcgctac gcgggcatcg
      721 gtgcctattc tggtcaactt tgtatccaag gccgttcgaa ttgctcgtga ttgtcctggc
      781 tgttttgcct tggatcgctg tctggctcgc gacacaatct ccaagccttt acagtttcgg
      841 aataacgggg cgcagcgttt cggctcggac aaggcccgat ctggggacac tattgctgct
      901 accgggcatg gctcttggtt tgcggggcca ctctgacgtg cacgttctcg aatggcaacg
      961 cacaatagtt tgcgcggcgg tcggcgcagc ggtgttgacc gctgcgctag ttcgcatcag
     1021 gccttctatg ttcgagaaca ccgcaaagtg ggtgctgttg gcgatcctat tggcacccta
     1081 cggatatggt gtaggggtaa ttgcgaacgc gcattgggat cgatcggttc ccac
//
LOCUS       DATCBB010000534         1150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_471775, whole genome shotgun sequence.
ACCESSION   DATCBB010000534 DATCBB010000000
VERSION     DATCBB010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471775"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VII37_05865"
     CDS             complement(<1..510)
                     /locus_tag="VII37_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5069289.1"
                     /translation="MKYCCFDVAGKAHYGLVESVAGRDEITRVLLTAPEDSSGDIEDL
                     PTRRMDRLPLTEALLLAPVRPSKIVCVGRNYREHAAELGNEVPTEPLIFLKPPSSLLA
                     PGGNIVRPKMSQRADYEGELGVVIGKKCHQLATDEDVDSYILGYTCVNDVTARDLQNK
                     DTQWTRAKGF"
     gene            complement(564..>1150)
                     /locus_tag="VII37_05870"
     CDS             complement(564..>1150)
                     /locus_tag="VII37_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY5069290.1"
                     /translation="EGIVVSWTPIPHPTAVSGLRYLYRIYRREENSKADAIAGELALD
                     SASGARFVDHNFASEQRYSYHATVVTLINRAPGPVAEVEGDDTPQVSVFTHDVFPPAV
                     PSGLQAAFTSEDKHSFIDLIWNPDTESDLAGYNVFRRESDGRPLKINSDLVKLPAFRD
                     QGVSAGKKYYYSVSAVDIRGNESARSEEANETVP"
BASE COUNT          268 a          328 c          293 g          261 t
ORIGIN      
        1 gaaccccttg gcgcgggtcc attgggtgtc tttgttttgc agatcgcgcg cggttacgtc
       61 gttcacgcag gtgtatccga gaatgtagga gtcaacgtct tcatctgtag ccagctgatg
      121 acatttcttg ccgatcacca cgccaagctc gccttcgtaa tccgcgcgct gggacatctt
      181 cgggcgaaca atatttccac ccggtgccaa taatgacgaa ggcggtttga ggaaaatcag
      241 cggctcggta ggaacctcgt tgccaagttc tgcggcatgt tcccggtaat tccgtcccac
      301 gcaaacgatt ttcgacggac gcaccggcgc cagcaataaa gcctcagtca gcggcagccg
      361 atccatgcgc cttgtgggca aatcttcaat gtcgccactg ctgtcttctg gtgcggtcaa
      421 cagcacgcga gtaatttcat cgcggccagc cacagattcg actaatccgt agtgggcttt
      481 acccgcgaca tcgaagcagc aatatttcat agcagatatc taaccacaaa gacttggatg
      541 ggacggaggg aaaagagagc ggcttacggc actgtctcat ttgcttcttc ggaccgcgca
      601 ctctcgttcc cgcgaatgtc cactgccgag acagaatagt aatatttttt tccagcggac
      661 acaccctgat cccggaaagc cggcagcttc accaaatctg aattgatttt caaaggccgg
      721 ccgtcgcttt cacgccgaaa aacgttgtaa cccgccaaat cgctttcggt gtcaggattc
      781 catataaggt caatgaagga atgcttgtcc tcgctggtaa acgctgcttg caatccggaa
      841 gggaccgcag gcggaaaaac gtcgtgcgta aaaacggaca cctgcggcgt atcgtctcct
      901 tccacttcag ccaccggccc cggcgcccga ttaattaacg tcaccaccgt agcgtgatac
      961 gaatatcttt gttccgacgc gaaattgtga tccacaaacc gcgcgccgga cgccgaatcc
     1021 aaggccaact cgcccgctat ggcgtcagct ttgctatttt cttcgcgccg ataaatgcga
     1081 tagagatagc gcaatcctga aactgcggtt ggatggggaa ttggcgtcca actaacgacg
     1141 atgccttctg
//
LOCUS       DATCBB010000535         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_374006, whole genome shotgun sequence.
ACCESSION   DATCBB010000535 DATCBB010000000
VERSION     DATCBB010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374006"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VII37_05875"
     CDS             complement(<1..327)
                     /locus_tag="VII37_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069291.1"
                     /translation="MFIPKMRSYKLFSLEISGALRALLLSGSLFVALWVLPACSIDAK
                     NKDKNGEAHVDIKSPVGDLHVSEEADIRDAGLTIFPGAKPAPKNDSDDKKSANVNLWL
                     PGFSLKV"
     gene            complement(314..>2086)
                     /locus_tag="VII37_05880"
     CDS             complement(314..>2086)
                     /locus_tag="VII37_05880"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEY5069292.1"
                     /translation="RKMSPEEEVGQLFSIWTRVQFLNDADPIFIQLSDSIRKYHVGSV
                     VMSVPVDGPLLLRNQPYIAAELLNRLQKASKLPLIVAADFERGLSMRLYGTTVFPHAM
                     AFSATGDAENAEAFGRISALEARAIGVHWNFFPDADVNSNPANPVINIRSFGEDPKQV
                     GDFVAAYIRGAHEAGMLTTAKHFPGHGDTATDSHLGLAQVSGDRARLHAVELPPFERA
                     ISAGVDAVMVAHVTVPALDSDPTRVATTSKAIVTGLLKEDMGFKGVVVTDALDMAGLT
                     RIYANDVGRAAVESFKAGNDVLIIPADLDASYRSMLQAVRSGEISRSRLDESVRKILE
                     LKASVGLNKARFADLSQLSREIAKPENVAVGQRIADEAITLVRDNGKVIPLQSSVNSV
                     GTPEAALPYQSVPVVSNRLVAVIFSDDLRTDSGRMLERQILARVRDAHVIYVDSRSAA
                     GMTPSVMQAIEGADHVIAALYVVPVAGRAIRTASGGVTNTVAVDDSMGALLNAILDRA
                     ADRTAVLAMGNPYVIQNFPAIQNYVCAFSNASVSETAAVKAMFGEIPIRGHLPVTIPG
                     IASRGEGMERTAQSNAGGSNNVHP"
BASE COUNT          466 a          613 c          570 g          437 t
ORIGIN      
        1 cactttcaac gagaatccag gcagccaaag atttacgttg gcgctctttt tatcgtcgct
       61 gtcgttttta ggagcaggct tcgctccggg aaaaattgtg agccccgcat cgcgaatatc
      121 agcctcctcg ctgacatgca agtccccgac cggagacttg atgtcaacat gagcttcgcc
      181 attcttgtct ttgtttttcg catcgatact gcaggcagga agaacccaga gcgcgacaaa
      241 aagcgaacct gacagcaaca gtgcgcgcag agcgccgctg atttcaaggc tgaaaagctt
      301 atagctacgc atcttaggga tgaacattgt tcgaacctcc tgcgttggat tgtgccgtcc
      361 gctccatacc ttcgcctctc gatgctattc caggaatcgt gaccggcaga tggccgcgga
      421 tcggaatttc gccgaacatc gccttgaccg cagccgtttc cgacacgctt gcgttggaaa
      481 acgcgcaaac ataattctgg atggccggga aattctgaat cacatacgga tttcccatgg
      541 ccagtacagc tgtgcgatcc gccgcgcgat caagaatcgc gttgagcaaa gcgcccatgc
      601 tgtcgtccac ggcaaccgta ttcgttacgc ctccactggc cgtccgtatc gccctgcccg
      661 ctaccggaac cacatacagc gccgcgatca cgtggtctgc tccctcgatc gcttgcatta
      721 cggatggcgt cattccggcc gctgagcggc tgtccacata aatcacgtgc gcgtcccgaa
      781 cgcgagccag aatctggcgc tcgagcatgc gtccagaatc cgtgcgcaga tcgtccgaaa
      841 aaataacagc gaccaaccgg ttgcttacca ccggcactga ttgatacgga agagccgcct
      901 ccggagttcc aacagaattt acagaagatt gcagcggaat tacctttcca ttgtcccgca
      961 ccagggtaat tgcttcatcg gcaatgcgct ggccaacggc tacgttctcg ggcttcgcaa
     1021 tttcgcggga aagctggctg aggtctgcga accgcgcctt gtttagaccc acggacgcct
     1081 tgagttccag aatcttgcgc accgattcat cgagccggga gcggcttatt tcgccgctgc
     1141 ggacggcttg cagcatcgag cgataactgg cgtcgagatc agcgggaatg atgaggacgt
     1201 cattgcctgc cttaaaagat tccactgcgg cccggccaac atcgttggcg taaatgcgcg
     1261 tcagtccggc catatcaagt gcgtccgtca ccacgacgcc tttgaatccc atgtcttctt
     1321 tgagcagccc ggtcacaatc gcctttgaag ttgtcgcgac gcgggtggga tcggagtcga
     1381 gggccggaac ggtaacgtgc gccaccataa ccgcatctac tcccgccgag atggcacgct
     1441 caaacggagg caattccacc gcatggagcc gggcgcgatc gccactaact tgagcgagac
     1501 caaggtgaga atcggtagca gtgtcgccgt gtccgggaaa atgtttggcc gtggtgagca
     1561 tgcccgcttc atgggcgccc ctgatatacg ccgccacaaa atcgcccacc tgcttcggat
     1621 cttctccaaa cgagcgaatg ttgatgacgg gattcgcggg gttggaattc acgtcggcat
     1681 cgggaaagaa attccagtgc actccgatgg ccctcgcctc aagcgcagag atacggccaa
     1741 aagcttcggc attctccgcg tcgccagtag cactgaaagc catggcatgg ggaaaaacgg
     1801 tggtgccata gaggcgcatt gacaggccac gctcaaaatc tgcggcaaca atcaacggca
     1861 gcttcgacgc tttctggagc cggttaagca actcggcggc aatgtagggc tggttccgaa
     1921 ggagcagcgg gccatccacc ggcacgctca tgaccacgga tccgacgtga tatttgcgga
     1981 tgctgtcgct caattgaatg aaaatcggat cggcgtcgtt tagaaattgc actcgcgtcc
     2041 aaatcgagaa gagttgcccg acctcttctt cgggcgacat cttgcg
//
LOCUS       DATCBB010000536         1891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_147759, whole genome shotgun sequence.
ACCESSION   DATCBB010000536 DATCBB010000000
VERSION     DATCBB010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147759"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VII37_05885"
     CDS             <1..844
                     /locus_tag="VII37_05885"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY5069293.1"
                     /translation="GPEKDVPAPDMGTNEQTMAWMMDTYSMHMRQTVTAVVTGKPINI
                     GGSRGRREATGRGVMIVCDEALRKFEMSREATRVIVQGFGNVGSNAAKLMQDAGYKVI
                     GIAEYDGGLFNRHGINIDNLLQHRDRNKTIVGFPDAEAADPKELLLADCEILIPAATE
                     NVITSQNAAGIKAKIICEGANGPTTAAADEILADKKVFIIPDILANAGGVTASYFEWV
                     QDRQGYFWKESSVNEQLEFILREAFEDVVHFSETHGVNNRIAAYMLAISRVAHTIKLR
                     GIYA"
     gene            complement(919..1344)
                     /locus_tag="VII37_05890"
     CDS             complement(919..1344)
                     /locus_tag="VII37_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069294.1"
                     /translation="MSFYDETLKARHEDDLDDDDSETGGYGSSSTEEDYDEEEEESIG
                     AESDGGAEESRGGEAPAPVSAPAPARKSGGGGGGKKKPAAKKPAAKKKPAAKKKPVAK
                     KKSASKKSGGSRGKKAPAKKAAKKKPVAKKKAAKKKGRR"
     gene            complement(1479..>1891)
                     /locus_tag="VII37_05895"
     CDS             complement(1479..>1891)
                     /locus_tag="VII37_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5069295.1"
                     /translation="AGVARKYRTTERAISQANNLQGTQLATETKLVIPVNGASANGKI
                     LYSRYASHYKTHSGDTVLTVSEDFGVPPDRLRRWNGLKGNDLLHGRVLVVYKPLAPGD
                     TDKAPVRRHKKTANSKARAAAASGQGTSLNAKSQ"
BASE COUNT          390 a          519 c          504 g          478 t
ORIGIN      
        1 tggcccggaa aaagacgttc ccgcgcccga tatgggcact aacgaacaaa ctatggcctg
       61 gatgatggac acttattcga tgcacatgcg ccagaccgtg accgctgtgg ttactggaaa
      121 accgatcaac atcggcggat ctcgcggccg ccgagaagcc acgggacgcg gcgtgatgat
      181 cgtctgtgat gaagcgctca ggaaatttga aatgagccgg gaagctacgc gggtgatcgt
      241 acagggcttt ggcaacgtgg gctcaaacgc cgccaagctc atgcaggacg cgggttacaa
      301 ggtcatcggc attgcggaat acgatggcgg attgttcaac cggcacggca tcaacattga
      361 taatctccta cagcatcgtg atcgcaacaa aacgattgtc ggcttcccag acgcggaagc
      421 tgctgatcca aaagaactgc tgctggccga ttgtgaaatc ctgattcccg cggccacaga
      481 aaacgtcatc accagccaga acgcggcggg catcaaggca aagattattt gcgaaggcgc
      541 taacggtccc accacggcgg ctgcagatga aatcctagcc gacaaaaaag tgttcatcat
      601 tccagacatt cttgccaacg ccggcggcgt taccgcttcc tacttcgagt gggtgcagga
      661 tcgtcaggga tatttctgga aggaatcctc cgtcaatgaa caactggagt tcatcctgcg
      721 cgaagccttt gaggacgtgg tgcacttttc cgaaacgcac ggcgtaaaca accgcatcgc
      781 ggcttacatg ctggccatca gccgggtcgc gcacaccatc aagctgcgtg gcatctacgc
      841 gtaacaaggc tgcgataaaa agaaacgcat aaaaaaggcc gcgataacct cgcggccttt
      901 ttcattgctt gattgttctt agcgacggcc ttttttcttg gctgcttttt tcttggcgac
      961 aggtttcttc tttgccgctt ttttcgcggg tgcttttttg ccgcgcgagc caccggactt
     1021 cttgctggcg ctcttctttt tagcgaccgg ctttttcttg gcagcgggtt ttttcttggc
     1081 cgctggcttc ttcgccgcag gtttcttttt tccgccgccg cctccgccgc tcttgcgtgc
     1141 gggtgctggt gcgcttactg gagctggagc ttctccgccc cttgactcct cagcaccgcc
     1201 gtcactttcg gcgccgatgg attcttcttc ttcctcatcg taatcctctt cagttgagct
     1261 gctaccgtag ccgccggttt cgctgtcgtc atcgtccaaa tcgtcctcat gtcttgcctt
     1321 gagggtctcg tcgtaaaagc tcatgtttcc tccagaaaat tagggccggg aaagcacaat
     1381 cacataaaaa attaacaggc gcggattata tcaagccaga tgatttgcgt gtcaacattc
     1441 gcgcatcaat tctgcaagcg atggaaaatt tatgctgctt attggctctt ggcgttaaga
     1501 gatgttccct gtcctgatgc ggcagcagct ctggccttcg aattggccgt ctttttgtgt
     1561 cttctcaccg gcgctttgtc tgtatctccg ggtgccagag gtttatagac caccagaact
     1621 cgtccatgaa gtaagtcgtt tcctttcaat ccgttccagc gtcgaagccg gtccggtgga
     1681 acgccaaaat cctctgagac ggtaagcaca gtgtcaccgc tgtgggtttt gtagtgtgaa
     1741 gcatagcggg aatacaggat cttgccgttg gcgctcgccc cattcaccgg gatcaccagt
     1801 ttggtttcag tggcgagctg agttccctgc aagttgttgg cttgggaaat ggcccgttcc
     1861 gttgtgcggt atttgcgtgc gacacccgcc a
//
LOCUS       DATCBB010000537         1945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_438830, whole genome shotgun sequence.
ACCESSION   DATCBB010000537 DATCBB010000000
VERSION     DATCBB010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438830"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="VII37_05900"
     CDS             complement(<1..1935)
                     /locus_tag="VII37_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5069296.1"
                     /translation="MNLSRPFILRPVATTLLMVGILLVGFVAYRQLPVSALPQVDYPT
                     IQVQTFYPGASPDVMASSVTAPLERQFGEIPGLNQMTSTSSFGSSIITLQFGLDLNID
                     IAEQEVQAAINGASNLLPSGLPNPPIYSKINPADAPILTLALTSNSLALSKVEDLADT
                     TLAQKISQLSGVGLVSISGGQRPAVRVQANPTALASYGLSLEDLRTALSQANVDQAKG
                     TFDGKHQAYTIGANDQLIGSDSYRPIIIAYRNGGAVRLSDVATVIDGSENVRQAAWAN
                     KNPAVILNIQRQPGANIISVVDRIKKLLPQLQSSMPAAIQVNILTDRTTTIRASVADV
                     QFELMLTIGLVVMVIFLFLRSVRATIIPSFAVPLSIVGTFGVMYLLGYSLNNLSLMAL
                     TISTGFVVDDAIVMIENITRYIEEGESPLQAALKGSAQIGFTILSLTVSLIAVLIPLL
                     FMGDIVGRLFREFAITLAVTILVSAFVSLTLTPMMCAKLLRHRARDEEGWFYRKSEDA
                     FNRVIAAYGRTLKVVLQYRTITLLVTVGTLVATILLYIYVPKGFFPVQDTGVILGVSE
                     GPQDVSFPAMARRQQALADVILRDPAVESLSSFIGVDGTNTTLNSGRIQINLKPLEQR
                     KINASDVIRRLQPEVAKVDGV"
BASE COUNT          462 a          537 c          556 g          390 t
ORIGIN      
        1 cacgccatcg actttcgcga cctctggctg caggcgtcga atcacgtcgc tggcattgat
       61 tttgcgttgc tcgagcggtt tcaaattgat ctgaatgcgc ccgctgttca gcgtggtgtt
      121 ggttccgtcg acgccgatga aggatgacag gctctcgacc gcgggatcgc gcaaaataac
      181 atccgccagc gcctgctgcc ggcgcgccat cgcgggaaac gaaacatctt gcggcccctc
      241 ggaaactccc aggatcacgc cggtatcctg caccgggaaa aatcccttgg gaacgtagat
      301 gtacagcaga attgttgcga cgagggtgcc aaccgtgact aacagcgtga tggtgcgata
      361 ttgcagcacc accttcagtg tgcggccata ggcggcaatc acacggttaa acgcatcttc
      421 cgatttgcgg taaaaccagc cttcttcgtc gcgcgcccga tgacgcaaaa gtttggcgca
      481 catcatgggc gtcaaagtca gcgagacaaa cgcggagacc aaaatagtca ccgccagcgt
      541 gatagcgaac tcgcggaaaa gtctgcccac gatgtcgccc atgaacaaca gcggaatcaa
      601 caccgcgatc agcgacaccg tcaaagacaa aatcgtgaat ccgatttgcg ccgagccttt
      661 cagcgccgct tgcaacggcg attcgccttc ctcgatatag cgggtgatgt tttcaatcat
      721 gacgatggca tcgtccacca cgaaacccgt gctgatggtg agagccatca aggaaaggtt
      781 attgaggctg tagcccagca ggtacatcac cccaaacgtg cccacgatgg aaagcggcac
      841 ggcgaagctg ggaatgatgg tggcgcgcac gctgcgcaga aaaagaaaga tgaccatcac
      901 caccagcccg atggtcaaca tcaattcaaa ttgcacgtcg gccaccgacg cgcgaatcgt
      961 tgtagtgcgg tcggtgagga tattcacctg aatcgcggca ggcatagaag attgcagttg
     1021 cggcaaaagc tttttgatgc gatcgacgac agaaataata ttcgcgccgg gctggcgttg
     1081 aatgttgagg ataaccgcgg gatttttgtt ggcccaggca gcttgccgga cattctcgga
     1141 gccgtcaata accgtagcca catccgaaag gcggaccgct ccaccgttgc gataggcgat
     1201 gatgatgggc cggtagctgt cgctgccgat cagttggtca ttggcgccta tcgtgtacgc
     1261 ctgatgcttg ccatcgaacg ttcctttggc ctggtcgaca ttggcttgac tcaacgcggt
     1321 gcgcaaatct tccagactca gcccgtacga agcaagtgcc gtgggattcg cttgcacgcg
     1381 caccgcggga cgttggccgc cgctgatgct gaccaatccc actcccgaca attgagaaat
     1441 tttttgcgcc agcgtagtgt cggcaagatc ttcgactttg gatagcgcca gcgaattgga
     1501 agtcagcgcc agagtaagaa tcggcgcatc cgcgggattg atcttgctgt aaatcggtgg
     1561 attgggcagc ccacttggca acaaattgga cgcgccattg attgccgcct gcacttcctg
     1621 ttcggcgatg tcgatgttta agtccaaacc gaattgcaaa gtgatgatcg agctgccgaa
     1681 tgaactcgtg gaggtcattt ggttcagccc gggaatttcg ccgaattgcc gctccagtgg
     1741 agcggtgacc gaagacgcca tcacatcggg gctggcgccc ggataaaacg tctgcacctg
     1801 gatcgtgggg taatcaactt gcggtaaagc ggaaacgggc agctgccggt aagcaacgaa
     1861 tcccaccaac aagatgccga ccatcagaag cgtggtcgcc accggccgta aaataaacgg
     1921 ccgcgaaaga ttcatcgagt ggcgc
//
LOCUS       DATCBB010000538         1696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_407431, whole genome shotgun sequence.
ACCESSION   DATCBB010000538 DATCBB010000000
VERSION     DATCBB010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407431"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VII37_05905"
     CDS             <1..286
                     /locus_tag="VII37_05905"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069297.1"
                     /translation="NKFEVMTLSTRSGLRYLSYLPTLYMGRLRGTNGVFFSKTDSLVC
                     EPLNGNAVHAQVDGEPLGRLPVEFKIVPGALRILVPEVASMHGRRTAASA"
     gene            321..1331
                     /locus_tag="VII37_05910"
     CDS             321..1331
                     /locus_tag="VII37_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY5069298.1"
                     /translation="MDIGTHALASLALSRAIFPRAPRLIWAFTIPAGVIADIDACSAL
                     ISPLAYLAWHRTYTHSLLASLIIAFIFSAAYRLWASRDLMSRLSAGKVFVGALLVQWL
                     HLAMDAAQWQGVELFWPMNSARIAMDWLPTIDPWIVTILVATILLPEFFHLIGAEIGA
                     KDKRPRGFVGAIVGLFTVICYVGLRAELHGSAVAQMQNRSYANELPRQVAAFSDFVSL
                     VTWHGVAETESALHEVMVHINSSRRSSLDPGINLFKPEANPILQAAQATEAAKYFLRV
                     ARFPKATIQKMDTGSEVQVRDLRYAAAGEVKREPMVTVDFDPSGKIISEEIVWASRET
                     PR"
     gene            complement(1338..>1696)
                     /locus_tag="VII37_05915"
     CDS             complement(1338..>1696)
                     /locus_tag="VII37_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069299.1"
                     /translation="GGAVRHWIERNGFGGMLAAALLPPPTPFKFFVFAAGVFEMPLAS
                     FTSAIAMARLFRYFGVGYLAVRYGDGALPFLQQHKLNVALAAVILVAISYGLSQLILR
                     AKHPYDDAAQKKPVED"
BASE COUNT          372 a          430 c          484 g          410 t
ORIGIN      
        1 aaataaattt gaagtgatga ctttgagtac gcggagcggt ttacgttatc taagttattt
       61 gccgactctt tatatgggca gattgcgcgg aacaaacggc gtatttttct caaagaccga
      121 ttctctagtt tgtgaaccgc tgaatgggaa cgcggtgcac gcgcaagtcg atggcgaacc
      181 gcttggacgc ctgccggtgg aattcaaaat cgtgcctgga gcattaagaa tattggttcc
      241 cgaagtggcg agcatgcatg ggcgacgtac cgctgcgtcc gcttagcgtt gctatgcttc
      301 ttgcacgtgg ctaacatttt atggacatag gcacgcatgc cttggcttcg cttgcgctgt
      361 ctcgcgccat ttttcctcgc gcgcccaggc taatctgggc cttcaccata ccagctggtg
      421 tgatcgctga tatcgatgca tgcagcgcgc tcataagtcc gttagcatat cttgcctggc
      481 atcgcaccta tacgcattcc ttattggcgt cgctgatcat cgcgttcatt ttcagcgcgg
      541 cgtatcgttt gtgggcatcg cgagatttga tgtcgcgatt gtctgcagga aaagtttttg
      601 tgggggcact gttggtccaa tggctgcatc tcgcaatgga tgcggcgcag tggcagggtg
      661 tcgaattgtt ctggcccatg aattccgcgc gcattgcgat ggattggtta ccgacgatcg
      721 atccctggat cgttacaata ttggtggcaa ccattctgtt gccggaattt ttccatttga
      781 ttggtgccga aattggcgcg aaggacaaac ggccacgtgg cttcgtcggc gccatcgtgg
      841 gattattcac ggtgatttgt tatgtcggct tgcgtgcaga actgcatggc tcggctgtcg
      901 cgcagatgca aaatcgcagc tacgccaacg aattgccaag acaggtcgca gcgttttccg
      961 actttgtttc gctggtcacc tggcacgggg tcgcggagac ggagagcgcg cttcacgaag
     1021 tcatggtgca cataaactcc tcacggcgtt cctcgctcga tccgggcata aatctattca
     1081 agccggaagc gaatccgatt ctgcaagctg cacaagccac ggaagcagca aaatactttt
     1141 tgcgcgtggc gcgctttccg aaagcgacta ttcaaaaaat ggacacaggc tcggaagtgc
     1201 aagtccgcga cttgcggtat gcagctgcag gagaagtgaa acgtgaaccg atggtgacag
     1261 tcgatttcga tccgtcggga aagataattt ccgaggagat tgtgtgggcg agccgggaaa
     1321 ctcctcggta ggatagccta gtcctcaacg ggcttttttt gtgccgcgtc gtcgtaggga
     1381 tgtttggcgc gcaaaattaa ctgggaaagt ccgtaactga tggccaccag tatcaccgcg
     1441 gcaagggcca cgtttaattt gtgttgctgc aaaaacggaa gcgcgccatc gccgtatcgc
     1501 accgccaggt aacctacgcc gaaatagcgg aacagccgcg ccatggcgat cgcggaagtg
     1561 aaactggcca gcggcatttc aaatactccg gcagcaaaca caaaaaactt gaagggcgtc
     1621 ggtggcggca acaatgcggc cgcgagcatt ccgccaaacc catttctctc tatccagtgg
     1681 cggactgcgc cgccgt
//
LOCUS       DATCBB010000539         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_147867, whole genome shotgun sequence.
ACCESSION   DATCBB010000539 DATCBB010000000
VERSION     DATCBB010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147867"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VII37_05920"
     CDS             complement(<1..809)
                     /locus_tag="VII37_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY5069300.1"
                     /translation="MTRRVVVTGVGLVCALGTGTEETWANLVAGQSGVGPITLFDTTG
                     FDCRIGGEIKNFDPFRWIEKKELKKMGRFIQVALAGADFAIKMSGWKREDSDLDEVGV
                     YVSSGIGGFDIIEREHIKLMQGGPGRISPFFIPSAIVNLASGHISIRYGARGPNSATA
                     TACSASAHAIGDSFKIIERGAAEMMICGGTEATITPMGVGGFSSMKALSTRNDDPSHA
                     SRPFDAQRDGFVVGEGAGILILESLEHAQKRNAPIVGEIVGYGMSGDAYHIT"
     gene            complement(848..1090)
                     /gene="acpP"
                     /locus_tag="VII37_05925"
     CDS             complement(848..1090)
                     /gene="acpP"
                     /locus_tag="VII37_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5069301.1"
                     /translation="MASVEERVKQIIVEQLGVDEAEVTPTASFVDDLGADSLDQVELV
                     MAFEESFGIEVPDEDAEKMTTVKDAVEYIDKHSKGK"
     gene            complement(1297..2043)
                     /gene="fabG"
                     /locus_tag="VII37_05930"
     CDS             complement(1297..2043)
                     /gene="fabG"
                     /locus_tag="VII37_05930"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY5069302.1"
                     /translation="MFSLKDKVALVTGASQGIGWDIAQALVVAGAKVAAAARTEEKLA
                     LLVSEIEAAGGEAIAVKMDVADAEQVKTGFKQVLEKFGRLDILVNNAAITRDGLAVRM
                     KPDDWDAVIRTNLTGAHLCIQQALGTMMRARAGRIINVSSVVAQMGNAGQANYVAAKA
                     GLIGLTKAIAMEIASRNITVNAVAPGFIDTPMTNVLSHKVKEELKARIPLGRLGVPRD
                     VAASIVFLASDEAAYITGHVLDVNGGMYLA"
     gene            complement(2116..>2911)
                     /gene="fabD"
                     /locus_tag="VII37_05935"
     CDS             complement(2116..>2911)
                     /gene="fabD"
                     /locus_tag="VII37_05935"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY5069303.1"
                     /translation="EDLKLTANTQPAILTCSIATYRVLEEKGLAPDFVAGHSLGEYSA
                     LVAAGALKFADAVRLVRKRGTYMQEAVPAGKGAMAAIMGLSPAVVTDVCKRASQGQVC
                     APANLNSPDQTVISGDADAVKRAVELASQAGAKRATILPVSAPFHSALMMPAQEKLAH
                     DLQKTHFDKLRVPLVTNVDADTIETGEQARDALVRQVTAAVHWEESVRLLIEEGVNTF
                     VEVGPGKVLTGLLRQIERSVATLNVEDETSLLKTVEKIAGARSDAA"
BASE COUNT          611 a          845 c          765 g          690 t
ORIGIN      
        1 gtgatgtgat aggcgtcgcc ggacattccg taacctacaa tctcgccgac gattggggca
       61 ttgcgctttt gcgcgtgctc taacgactcc agaatcaaaa tgcccgcacc ttcgccgacc
      121 acgaaaccgt cgcgttgcgc atcgaagggg cggctggcgt gcgagggatc gtcattgcgc
      181 gtggaaagcg ctttcataga tgaaaagccg ccgacaccca tgggggtgat ggtggcttcc
      241 gttccgccgc aaatcatcat ttcggcggcg ccacgctcga taattttgaa agaatcgcca
      301 atggcgtgcg cggaagcgga gcaggccgtg gcagtcgcgg aattcggccc gcgcgcaccg
      361 taacgaatgg aaatgtggcc ggaagcaaga tttacgatag cggagggaat aaagaaggga
      421 gagatgcggc ccggaccgcc ctgcatcaat ttaatatgtt cgcgctcgat gatatcgaaa
      481 ccaccgatgc cggatgaaac gtaaacgcca acttcgtcca ggtcggaatc ttcccttttc
      541 caaccggaca tcttgatggc aaagtccgcg ccggcgaggg ccacctgaat gaagcggccc
      601 atttttttta attctttttt ttcaatccag cggaagggat cgaaattctt tatttcgccg
      661 ccgatcctgc aatcgaatcc cgtagtatca aacagcgtga tcggacccac gccgctttgc
      721 cccgcgacga ggttcgccca ggtttcctcc gtgccggtgc ccagcgcgca gaccaatcca
      781 acgcccgtca caactacacg gcgagtcaac tgtttggtgc tccctgttga ggtgataaga
      841 ccgtgactta ctttcccttg gagtgcttat cgatatattc gacggcgtct tttaccgtcg
      901 tcattttctc ggcgtcttcg tcaggcactt caatcccgaa actttcttcg aaggccatca
      961 ctaattccac ttggtcgagc gaatccgcgc ccaaatcgtc cacgaaagac gctgtgggcg
     1021 taacttcggc ttcatcgacg ccgagctgct cgacaataat ctgcttaacg cgttcttcca
     1081 cactggccat tcctgtttcc tccgcgatct gctatcgctt ggtttgcctt ttaccgatca
     1141 ggctcaaggt cgttctggaa aattggtatc cgctcagccg ggcgtagtgg tgccagccta
     1201 ctgccgatat cgatggcgcc gataacccgt tcattctagt caccgtatcc cgctaggtca
     1261 actttaacgg ctctgttaag gtcgccgtcc gaaaaattat gccaggtaca tgccgccatt
     1321 tacatccagc acatgacctg tgatgtaggc agcttcatcg ctagccaaaa agacgatcga
     1381 ggcggccaca tcacggggga cgcccaagcg cccgagaggg atgcgcgcct tcagctcttc
     1441 tttgactttg tgggataaaa cattggtcat gggcgtatcg ataaatccgg gcgccacggc
     1501 gtttacagta atgttccgcg aagcgatttc catcgcgatg gctttcgtta gtccgatgag
     1561 cccggctttc gcggccacat aattggcctg cccggcattg cccatttgcg ccaccacgga
     1621 gctcacgttg atgatgcggc cggctcgcgc gcgcatcatg gtacccagcg cttgttgaat
     1681 gcataaatgt gcgccagtca gattggtgcg aatcaccgcg tcccagtcat ctggtttcat
     1741 gcgcacggca aggccatcgc gagtgatggc cgcgttattg actagaatat ccaggcggcc
     1801 aaatttttcg agtacctgct tgaacccggt ttttacttgt tccgcatcgg caacatccat
     1861 tttcacggcg atggcttcac cgcccgccgc ttctatttcg cttacgagca acgcgagttt
     1921 ttcttctgtg cgcgcggcgg cggcaacttt ggcgccggca actaccagcg cttgcgcgat
     1981 gtcccatccg attccttggg aggccccagt taccaacgcg actttgtctt ttaaactgaa
     2041 catctataga actgctccct gaaactttta aatcacaacg ttcgccgatt gtgactgcgt
     2101 aatttttcgt agtctctacg ctgcgtccga acgtgcgcca gcgatttttt caaccgtctt
     2161 caataaactt gtctcgtctt ccacgtttaa ggtggccaca gaacgttcaa tttgccgcag
     2221 cagtccggtg agcactttgc ccggaccaac ttcaacgaag gtgttcacgc cttcttcaat
     2281 cagcaggcgc accgattctt cccagtgcac ggcagcagtt acctggcgca ccagtgcatc
     2341 acgggcctgc tcgccggttt cgatggtgtc ggcatcgaca ttcgtgacta gtggaacgcg
     2401 caacttatcg aaatgagttt tctgcaagtc gtgcgctaat ttttcctgtg caggcatcat
     2461 cagcgcgcta tgaaatggtg cagacaccgg aagaatggtg gcgcgttttg cgccggcctg
     2521 agaagctaat tcaacggcgc gtttcacggc gtccgcgtcg cccgagatga ccgtttgatc
     2581 gggagaattt aaattcgcgg gagcgcaaac ctgaccttgt gacgcacgct tgcaaacgtc
     2641 cgttaccaca gcaggagaaa gtcccatgat cgcagccatc gcgcctttac cggcgggaac
     2701 ggcttcctgc atataggtgc cgcgcttgcg cactaagcgc accgcatccg caaatttcaa
     2761 cgcgccggcg gccaccagcg cagaatattc gccaaggctg tgaccggcca caaagtcggg
     2821 ggcgaggccc ttctcttcca gcacgcgata cgtggcaatc gaacaggtta gaatcgccgg
     2881 ttgcgtgtta gcggtaagtt tgaggtcttc t
//
LOCUS       DATCBB010000540         1608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_309652, whole genome shotgun sequence.
ACCESSION   DATCBB010000540 DATCBB010000000
VERSION     DATCBB010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309652"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VII37_05940"
     CDS             complement(<1..377)
                     /locus_tag="VII37_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069304.1"
                     /translation="MLRSRRVEDLFYLLAVVQIAVGAYLIWQGVQWLGYARRRMRTDP
                     GFHAPRVAVLCPCKGVEPGLERNLVSLTEFNHQNYEVFFIVASASDPAVPILNRVISS
                     SRPKAHLIVAGQPQNCGEKVNNL"
     gene            450..797
                     /locus_tag="VII37_05945"
     CDS             450..797
                     /locus_tag="VII37_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY5069305.1"
                     /translation="MTMPRVNTAMVTSALELPGYRIVKNYGIVRGIIVRSRSVIGNLG
                     AALQTIVGGNITILTDLCEKTREDAFELMLQHALEHGANAVVSMRYDATEVMQGVTEV
                     LAYGTAVHVEPIA"
     gene            complement(1004..>1608)
                     /locus_tag="VII37_05950"
     CDS             complement(1004..>1608)
                     /locus_tag="VII37_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069306.1"
                     /translation="RMRSQPQNVPDLASELAQRLPEALVGERVIPRFVDSYVVEHGRH
                     ALQAHASQYRDRLALLQREALLALTVQALSAACFEPPASKKSKARPMSQKDAAIFRRK
                     FLSALARQQKWSVGDALDFQTDLQIYENLIRRNSATRRTRKSFEVANHPFVDRSAFLL
                     DSSFMEKARIAASRALGDLEDLAAHVVASAGSRKAAAKLR"
BASE COUNT          370 a          448 c          453 g          337 t
ORIGIN      
        1 ctcagattgt tcaccttttc tccgcagttt tgcggctgcc ccgcgacgat taggtgcgct
       61 ttcggacgcg aactggaaat gacgcggttc aaaatgggga cggcgggatc cgatgcggaa
      121 gccacaataa aaaacacttc atagttttga tgattgaatt ccgtgagcga aactagatta
      181 cgctctaatc ccggttcaac gcccttgcac ggacagagca ccgcgactcg tggcgcatga
      241 aatccaggat cggtgcgcat ccgccgtcgt gcgtagccga gccactgtac gccctgccaa
      301 ataagatagg cgccgacggc aatttgcacg accgccagta aatagaagag gtcttccacg
      361 cgccttgagc gtagcaaagt aagattgctt gggtttgatt atctgtgcgg ttagaatcgc
      421 agcggtaaac gtcaaataca ggaggaacta tgaccatgcc cagagtcaat accgctatgg
      481 tgacctcggc gctggaattg cccggatatc gcattgtcaa aaattacggc atcgtacgcg
      541 gaatcatcgt gcgctcacga agtgtaattg gaaatctggg cgccgccttg cagaccatcg
      601 tgggaggaaa tatcaccatc ttgaccgacc tgtgcgagaa gacccgcgaa gacgccttcg
      661 aattaatgtt gcagcatgca ctggaacacg gagcgaacgc cgtggtatcc atgcgttacg
      721 acgctacgga agtgatgcaa ggcgtgacgg aagtcctggc ctacggaacc gccgtgcatg
      781 tcgagcccat cgcgtaacta cctcgaatgc tcctgtgcaa aatcgcgtaa gcggcgacac
      841 ccgtatgcat gtgctacgag acgcgaagtg atgcaaggcg tgacggaagt gttggcctac
      901 ggaaccgcga tgcatgtcga gccctcgcgt aagtcccgct agtgagcgtg cgtatctttg
      961 taagcggcga tcgaacgaac gcgtattgca cctttatcgc ggctcaccgt aattttgcgg
     1021 cggccttacg ggaccccgcc gaagccacta cgtgcgcggc aaggtcttcc aggtcgccaa
     1081 gcgcacggct tgccgcaata cgtgcttttt ccatgaatga cgaatccaac aggaacgccg
     1141 agcgatccac aaacgggtgg ttggctactt cgaaagattt acgcgtacgc cgggtcgcgc
     1201 tattgcgcct gatcaaattc tcgtaaatct gcagatcggt ctgaaaatct agagcgtccc
     1261 caacgctcca tttctgctgg cgcgccagcg cactcaaaaa tttgcgccgg aatatcgccg
     1321 catctttttg cgacatcggc cgagccttgg acttttttga agccggcggc tcgaagcaag
     1381 cggcggaaag cgcctggacg gttaacgcca acagcgcttc acgttgcagc agtgcgagtc
     1441 gatcgcgata ttgtgacgcg tgcgcctgca gtgcgtgccg cccgtgttcc accacatagg
     1501 aatccacgaa acgcggaatg acccgctctc cgaccagcgc ttcgggcagt ctttgcgcta
     1561 gttcacttgc caggtcagga acattttgcg gctggctgcg catgcgga
//
LOCUS       DATCBB010000541         1526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_341878, whole genome shotgun sequence.
ACCESSION   DATCBB010000541 DATCBB010000000
VERSION     DATCBB010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341878"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VII37_05955"
     CDS             complement(<1..1192)
                     /locus_tag="VII37_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5069307.1"
                     /translation="MDTCSDPDRAVERLVVLLIIAFFVFRIALAVTLGLGVDESYGIS
                     VAHDLRLSYFDHPPLHYWIAHFFMPLLGDGRAARLPFDVIFIGTTWALYLLTRQLFGA
                     IAGFWAVLALNLSAFFTLAGGWVLPDGPLMLSLVAAAYTIARALFPEHAPPSPWRTWI
                     AAGIWIGIAALSKYHAVLFVGGLFIFVVSMPTRRNILWHPAPWVGATIALLIASPVII
                     WNAEHHWVSIVFQAGRANAHGFPKIGQFLANIGGQILWMLPWIFVPLAIASYNALRMG
                     RGAERSWYCLCLAMPAIIIFTVVPLWGDRGLPHWQMPGWLMLFPVLGEHLAREAAVRN
                     RPRTWAITSAALLLLLAVLIVGHAATGYGRILFPRLLAKHDPTLESLEWSPLRAELES
                     RGLLK"
BASE COUNT          416 a          416 c          425 g          269 t
ORIGIN      
        1 gcttgaggag tccgcgagat tccagttccg ctcgcagtgg ggaccactca agcgattcca
       61 aagttggatc gtgcttggct aggagcctcg gaaataggat tcgaccgtaa ccagttgccg
      121 cgtgaccaac gatcaaaacg gccagcagga gcaatagcgc tgcggatgtg atggcccaag
      181 ttcgcggccg gttccgcact gcggcttccc gcgcaaggtg ttcacccaat accggaaaca
      241 gcatgagcca gccgggcatt tgccaatgcg gtaggccgcg gtcgccccac aatggtacga
      301 cagtgaagat gattatcgcc ggcatcgcca aacaaaggca gtaccatgag cgttcggcgc
      361 cacgtcccat gcgcaaggca ttgtaggacg cgattgccaa ggggacgaaa atccatggca
      421 gcatccaaag gatttgaccg cctatattgg caagaaactg cccaattttc ggaaagccat
      481 gggcgtttgc gcgtccggcc tggaagacga tcgacaccca gtgatgttcg gcgttccaga
      541 tgatcaccgg tgaggcaatg agcaatgcaa tcgtcgcgcc tacccaaggc gcagggtgcc
      601 aaagaatatt ccgccgcgtc ggcatgctga caacgaatat gaacagccca ccaacgaaca
      661 acaccgcgtg atattttgaa agcgcggcga tgccgatcca tattccggca gcgatccagg
      721 ttcgccaggg cgatggtggc gcgtgttccg gaaacagcgc tcgcgctatc gtgtaagcag
      781 cggcgaccaa agaaagcatc aatggaccgt ctggcagaac ccagccacca gctagcgtga
      841 aaaatgccga gagattgagc gccagcactg cccagaagcc ggcgatcgct ccgaacaatt
      901 gacgcgtcag cagataaagc gcccaggttg tcccgataaa gattacatcg aatggcaggc
      961 gcgccgcacg cccatcacct agcaacggca tgaaaaagtg cgcaatccag taatgcagcg
     1021 gcgggtggtc gaagtaagaa agtctgagat cgtgggctac actgatcccg tagctttcgt
     1081 cgacgccgag cccaagcgtc accgctagag cgatacgaaa tacgaaaaat gcaatgatta
     1141 gcagaacgac cagtcgctca accgcgcgat ctggatccga acaagtgtcc aattctgaga
     1201 tcctcaaaag caatccagca ggttagaaag ctaaccgcct aacttcacgc agctagcgga
     1261 gaatccccgt gcaagcaacg gaatttgcgg gcggctaagg gacaaattca agctacgtga
     1321 gctaacgcga tgtccgaaaa agtagaccgt ttaggtgacc taggagtgac caagttgcgg
     1381 actgcatgtc cttttggttg catcgctaaa aagcaagaaa ccaaaaaaat gcacaattca
     1441 caattttatt gggaagtttt gcgcatacaa tcgcaaccta tggtgggcgc accagggcca
     1501 gaacctggga cccgctgatt aagagt
//
LOCUS       DATCBB010000542         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_374261, whole genome shotgun sequence.
ACCESSION   DATCBB010000542 DATCBB010000000
VERSION     DATCBB010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374261"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VII37_05960"
     CDS             <1..347
                     /locus_tag="VII37_05960"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069308.1"
                     /translation="SQLPGLITFERTVHKRPRGKVLMDALQNARGKPLATVYSARAYP
                     GAPVSTPVTPTELKTEFTADRWDLRNVEQRIKKVGNLWGDFWNKRQPLSEALDLLEQR
                     LSLGKKPAGKTR"
     gene            complement(366..860)
                     /locus_tag="VII37_05965"
     CDS             complement(366..860)
                     /locus_tag="VII37_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5069309.1"
                     /translation="MPVRILVADDHEVVRQGIRTILSARPDWEICGEAVNGQEAIKLA
                     DDLRPDAIIMDITMPVMSGLEAARQISRNKTSPPILIFTMHESRSLADSVRETGARGF
                     VFKSRAARDLINALDVLLQNGTYFGPPGDKQDAPPKEDTNRGISFLRALDFLKRPAFA
                     SDLA"
     gene            complement(1090..2724)
                     /locus_tag="VII37_05970"
     CDS             complement(1090..2724)
                     /locus_tag="VII37_05970"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY5069310.1"
                     /translation="MRRFVETCDAVRATTKKSIKVTLVSELFKSLPVEDAATAAIFLT
                     GRPFPHRDERVLGVGGSQLAKIVTEIAGADASDLGKSYRAHGDLGDMAEHLLLKRPST
                     ADVSLQQVAKTFSQLPEVRSSAQKMALIKTLFEHMSAGEVKYAIKIMTGDLRIGLKES
                     LVEEAIAKAFDRPLDAVRRANMLTGDIGETLNSAAANHLAAVRMRLFHPIGFMLATPV
                     ETASEILPDGHGSVLVEEKYDGIRAQVHKSATGVKVFSRTLDQIAEFPELDAFFAKLP
                     GDLILDGEILAWHGSRPLPFTELQKRLGRKQLDLWLQHEIPVRFAAFDLLYQDGTLLL
                     EETLQQRRGRLEKLLSLGGSSAIYTATAIGCDSVSAIQQVFQDSLAAGHEGIVVKRPE
                     SLYTPGRRGRSWFKLKEPFATLDVVVTAVEYGHGKRHGLLSDYTFSIRHGHQLLTIGK
                     AYSGLTDAEIKAGTEYFLQHTVEDLGARRIVEPAVVMEVAFNNIQKSQRHESGYALRF
                     PRILRLRPDKPVVEIDTLERVAQLYARQITLPRNNQ"
     gene            complement(2729..>3104)
                     /locus_tag="VII37_05975"
     CDS             complement(2729..>3104)
                     /locus_tag="VII37_05975"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY5069311.1"
                     /translation="LTQSLLSGIGNVFKSEICFACGVNPFRLVSDLSDQDLQRIVSTS
                     RKFMLANVTEDSGDKIVTYVPMRHTTGRINIEERLWVYKRTGEPCRNCGSAIVSRKQG
                     FDARTSFWCPRCQPMGMQRASA"
BASE COUNT          718 a          777 c          900 g          709 t
ORIGIN      
        1 acagccagtt gcccgggctt attacattcg agcgaacggt gcacaaaagg ccgcgcggaa
       61 aggtgttgat ggatgcgctg caaaacgcac gcgggaagcc gctcgcaacg gtgtattcag
      121 cgcgcgccta tccaggtgcg ccggtgtcaa cgccggtgac tcccacggaa ttaaaaacgg
      181 agtttaccgc tgaccgctgg gatttgagga acgtggagca acgaatcaag aaagttggaa
      241 atttatgggg agatttctgg aacaagcggc aaccgttatc ggaagctctg gacttgttgg
      301 agcagcgact atcgctggga aagaaacccg cgggtaaaac gcgctagctt gaagcaggca
      361 attgcctaag cgagatcgct ggcaaacgct ggacgtttca agaaatccaa agcgcgcaaa
      421 aagctgattc cgcggttggt gtcctctttc ggtggggcat cctgtttgtc gcctggaggg
      481 ccgaaatacg ttccgttctg tagcagaaca tctaaagcat tgattaaatc gcgggccgcg
      541 cgtgatttaa acacaaatcc gcgagctccc gtctcacgca cggaatctgc gagactgcga
      601 gattcatgca tggtgaaaat gagaatggga gggctagttt tattcctgct aatttgtcgc
      661 gcggcttcca ggccgctcat caccggcatg gtgatgtcca taatgatggc gtcggggcgc
      721 agatcatcgg caagcttgat ggcttcctga ccgttaacgg cctcgccgca aatttcccag
      781 tcgggacgag cggagagaat cgtgcgaatc ccctggcgca ccacttcatg atcgtcggca
      841 acaagaattc taactggcat aagaatctac tccgtagaca tttgtcgcgg cctgtcgcta
      901 caaacctgga acagcagaac agcaattaaa attatatggg acagttaccc gagatgtacc
      961 gtcctcgggt tgtggcaccg ggaaagcctg aataatttat atacaggatt tctgttccgc
     1021 gcaagtgtgg cattaatcat gacgacacat agttatcttc gggtcttggc gccctgagag
     1081 gccgcgatgt tattgattat ttctgggcaa ggtaatctgc ctggcgtaaa gctgggcgac
     1141 gcgctcgagg gtatcgatct ctacaacagg tttgtcgggg cgaagtcgca aaatgcgtgg
     1201 aaatctgagt gcgtagccgc tttcgtggcg ctgcgacttc tgaatgttgt tgaaagccac
     1261 ttccatgact acggcgggct caacaattct gcgcgcgccg agatcttcga cggtgtgttg
     1321 caggaagtat tctgtaccgg ccttgatttc ggcgtcagtg agcccagaat aggctttccc
     1381 aatggtcaag agttggtggc cgtggcgtat tgaaaaagtg tagtcagaaa gcaggccatg
     1441 ccttttgccg tggccatatt cgactgcggt cacaaccacg tcgagggtgg cgaagggctc
     1501 ctttagcttg aaccaggacc tgccgcggcg tcccggggtg tataacgatt ctgggcgttt
     1561 tacgacgatt ccctcgtggc cggcggccag cgaatcctgg aagacctgtt gaatggccga
     1621 aaccgaatca caaccaattg ccgttgcggt atagattgcc gacgagccgc caagggacag
     1681 aagtttttca agccgtccgc ggcgttgctg cagcgtttct tccagcaaca aagttccgtc
     1741 ctggtaaagc aaatcaaagg cggcaaaacg cacgggaatt tcgtgttgca gccatagatc
     1801 aagctgtttc ctgcctagtc gcttttgtaa ctccgtgaat ggaagaggtc gcgaaccgtg
     1861 ccaggctaga atttcgccat cgaggatcag gtcaccaggc aattttgcga agaaagcatc
     1921 gagctcggga aattccgcga tctggtcaag cgtacgcgaa aaaactttta cacccgtggc
     1981 gcttttatga acttgtgcgc gaatgccgtc atacttttcc tcgaccaaaa ccgagccgtg
     2041 cccatctgga agtatttcgc tggcggtttc cacgggcgtg gccaacatga aaccgatggg
     2101 atggaagagg cgcattcgaa cggcggctaa atggttcgcg gcggcggagt tcaaggtttc
     2161 gccaatgtcg ccggtgagca tattggcgcg gcggacggca tccaaggggc ggtcgaaggc
     2221 tttggcgatg gcttcttcga cgaggctttc cttcaatccg atgcgtaaat cgccggtcat
     2281 tatttttatg gcgtacttca cttcgccggc gctcatgtgc tcgaataaag tttttatcaa
     2341 agccatcttt tgagctgagc tgcgaacttc tggaagctgg ctgaacgttt ttgctacctg
     2401 ctgcagcgag acgtcggcgg tgcttgggcg tttaagcaag agatgttcag ccatgtcgcc
     2461 gaggtcacca tgggcgcgat aactttttcc gaggtcggag gcgtcagcac cggcgatttc
     2521 ggtaacgatc ttcgccagtt gcgagccgcc tacgcccaag acacgttcat cgcgatgtgg
     2581 gaaagggcgg ccagtcagaa aaattgccgc ggtcgcggca tcttccacgg gcagcgattt
     2641 gaagagttcg cttacaagtg tgaccttgat gctttttttg gtggttgcgc gtacagcatc
     2701 acacgtctct acgaaccggc gcatgaagtt aagcgcttgc gcgctgcatc cccatgggct
     2761 ggcagcgcgg gcaccagaag gaagtgcgcg catcaaagcc ctgtttgcgt gaaacgatgg
     2821 cgctgccgca attgcgacaa ggctcaccgg ttcttttgta gacccacaag cgttcttcga
     2881 tattgatgcg gccggtggtg tggcgcatag gaacataagt aacaattttg tcgccagagt
     2941 cttcggttac gttggcgagc ataaatttgc gcgatgtgga gacgatacgc tgcaagtcct
     3001 ggtcgctaag atccgagacc aggcgaaacg ggttaacgcc gcacgcaaaa caaatttccg
     3061 acttaaaaac atttccgatc cccgaaagca acgattgggt aagc
//
LOCUS       DATCBB010000543         1711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_341955, whole genome shotgun sequence.
ACCESSION   DATCBB010000543 DATCBB010000000
VERSION     DATCBB010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341955"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VII37_05980"
     CDS             complement(<1..208)
                     /locus_tag="VII37_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069312.1"
                     /translation="MPRYEYRPLTPSPEAEKNFLEWIAKLNQEFENSDPNHRSDVVRD
                     ALYQIFLGSPYEPPKPGDAPGTQAL"
     gene            complement(263..853)
                     /locus_tag="VII37_05985"
     CDS             complement(263..853)
                     /locus_tag="VII37_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069313.1"
                     /translation="MKANASLLLVVLLALFFIGPDASIADASQNAAAVRAGAKLQHIE
                     KNAGLARPDRAPTDLTDDEINAYFAAGKVKLPVGVQSVRFQGQPGIVTASCRVDFDQI
                     KAGRHTANPLLSLFSGIHDVVVVANAKGAEGQGLVEVQSVLLDEVEIPRFILELFVEK
                     FLQPKYPSVGLESRFALPQKIDTAIVGANKLNITQK"
     gene            complement(850..>1711)
                     /locus_tag="VII37_05990"
     CDS             complement(850..>1711)
                     /locus_tag="VII37_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY5069314.1"
                     /translation="DSASVAVLPNSVWRGSLIMPRPCGVAGMCPIQISLHADEISTAQ
                     LGSLFNPQNSKAPWYQFLMASQRDSYLLGLRATGQLTADRILVHKLAGTHFSTSFELD
                     RGKLHLADVRATVFGGMHSGDWRLDFTVRPPAYSGEGSFQNVVLTQFASAMHDNWISG
                     TASATYHATAFGSNAADLFSSATGIIQIEARDGSMPHIVLANFAGPLQLRMLKGNLLL
                     RRQELDFTDAKVETNAGTYRVAGTASLGRTLNLKLTRSGSRGFNIAGTISAPVISTNS
                     IPETQAALKP"
BASE COUNT          395 a          475 c          453 g          388 t
ORIGIN      
        1 tcagtgcctg tgttccaggc gcatcaccag gcttcggcgg ttcatatggg cttcccagga
       61 aaatttggta aagcgcatcc cgtaccacgt cgctgcggtg attgggatct gaattctcga
      121 attcttgatt taattttgcg atccactcta aaaaattctt ctcggcttcg gggctcggag
      181 taagtggacg atattcgtat cggggcatcg cacctcgcct cttcaattca gacgataaaa
      241 catagcacag tctcgcgcgg cattactttt gggtgatatt cagcttgttt gcgcccacta
      301 tcgctgtatc aatcttttgc ggcagggcga agcgggattc aagccctacg ctgggatact
      361 tgggttgaag gaatttttca acgaacagct ccagaatgaa tcgcggaatt tcgacttcat
      421 caagcaacac cgattgaacc tcaaccagac cctgcccttc ggcacctttc gcgtttgcca
      481 cgacgaccac atcgtgaata cctgaaaaaa gcgacaacaa tggattagcc gtatgccgtc
      541 ccgctttgat ctgatcgaaa tccacgcggc aggacgcggt cacaatcccc ggttgtccct
      601 gaaaccttac cgactgcact cccacaggca gcttcacttt gccggcggca aaatacgcat
      661 ttatttcatc gtcggtgagg tcggtgggcg cacgatccgg ccgcgccaga ccggcatttt
      721 tctctatatg ttgcaacttc gccccggcgc ggaccgccgc cgcattttgc gaagcatcgg
      781 caatggaggc atccggcccg atgaaaaata gcgctaatag gacgaccagc aggagcgaag
      841 cgttcgcctt catggcttca aagctgcttg cgtctctgga attgaattcg ttgagataac
      901 gggcgcgctg attgttccgg cgatgttaaa ccctcgactg ccactgcgcg tcagtttcaa
      961 gttgagcgtg cgccctaaag aagcggtgcc agccacccgg taggtaccgg cgttggtttc
     1021 gaccttagcg tctgtaaaat cgagttcctg gcggcgtaga agtaaattgc ctttcaacat
     1081 gcgcagttgt aacggtcccg cgaaatttgc caacactatg tgcggcatag atccatcgcg
     1141 ggcttcgatt tgaataattc cggtagcgga cgaaaacaaa tccgccgcgt tcgagccgaa
     1201 ggcggtcgca tgataggttg ctgaggccgt ccccgaaatc cagttgtcgt gcatcgcgct
     1261 tgcgaactgc gtcaacacaa cattctggaa agaaccttct ccactatatg ccggagggcg
     1321 caccgtgaaa tccagcctcc agtcgccgct gtgcatgccg ccaaataccg tggcccggac
     1381 gtccgccaaa tgcagcttcc cacgatccaa ctcaaaactg gtggaaaaat gagtgccggc
     1441 gagtttgtgc acgaggatgc ggtcggcagt gagttgtcct gtcgcgcgaa gtcccagaag
     1501 atacgaatcc ctctgcgatg ccatcagaaa ctgataccag ggcgctttgg aattttgcgg
     1561 gttgaagagc gagcccagtt gggcggtcga aatctcatcg gcgtgaaggc tgatctgaat
     1621 aggacacatt cccgcaaccc cgcatggacg gggcattatc aatgagccgc gccaaaccga
     1681 attaggcaat actgcgacgg acgctgaatc c
//
LOCUS       DATCBB010000544         1475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_82286, whole genome shotgun sequence.
ACCESSION   DATCBB010000544 DATCBB010000000
VERSION     DATCBB010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82286"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VII37_05995"
     CDS             <1..433
                     /locus_tag="VII37_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5069315.1"
                     /translation="AMLALMLFKQDLNVISIIGIVLLIGIVKKNAILMIDFAVQVERE
                     GHKSPVEAIYEACLLRFRPILMTTMAALLGALPLALGSGVGSELRRPLGITIVGGLIL
                     SQMLTLFTTPVVYIYMDRLQTFIQSLRRHGASATPAPAQSD"
     gene            509..1045
                     /locus_tag="VII37_06000"
     CDS             509..1045
                     /locus_tag="VII37_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069316.1"
                     /translation="MAGIDYVEPSHAGPERAIEADHRVRRLPPSAFPELPKKINQELE
                     RRGCTIPQVPSDKKPHNVISGEFTGKGRTDWAVLCSLNRVSTIVIFRNASEREPLEIA
                     PQADADSLQRGGGTAIEYSRAIEPVGREYILKHYRAYGGPEPAAIDHQGINDSFVGKA
                     SVVRYFHAGKWLELTGAD"
     gene            complement(1107..>1475)
                     /locus_tag="VII37_06005"
     CDS             complement(1107..>1475)
                     /locus_tag="VII37_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069317.1"
                     /translation="ALQERAKEIPNLISPHLGDSIPNSSTIASSAISRQPDSKSASNV
                     LAALPLGGRIIVQPWDDRLVMRKVEPEGAIAEFEKMPFEVAPFLIYLTRPSANSANAS
                     GVVAVKSEIDMDQPLPLSKP"
BASE COUNT          339 a          406 c          400 g          330 t
ORIGIN      
        1 cgccatgctc gcgttgatgt tgttcaagca ggatctaaac gtgatttcta ttattggcat
       61 tgtgctgctg atcggcatcg tcaagaaaaa cgccatcctg atgatcgatt ttgccgtgca
      121 ggtggaaaga gaagggcaca agtcgccggt ggaagcgatt tatgaggcgt gcctgctgcg
      181 cttccgcccc attttgatga ccaccatggc ggcgttactt ggcgctttac cactggcatt
      241 aggtagcggc gtaggctccg agttacgccg cccactgggc atcaccatcg taggcggatt
      301 aattctcagc caaatgctca cgctgttcac cacgccggtg gtgtacatct acatggaccg
      361 cctgcaaacc ttcattcaga gcttgcgcag gcacggcgca tccgccaccc cagccccagc
      421 acaaagcgat tagttacggc agtgaggatt tggagatgcg atcatgcatt gctgtcacca
      481 ggcggccctg agcgcgatta tctttctgat ggcgggcatt gattacgtcg aaccttctca
      541 cgcaggcccg gaaagagcta tagaagcaga tcatcgggtt aggcggcttc caccctctgc
      601 gtttccggaa ttaccaaaaa aaataaacca agaactcgaa cgccggggat gcacgatacc
      661 gcaggtaccg tctgacaaga agccacacaa cgtaatcagt ggcgagttca ccggcaaagg
      721 acgcaccgac tgggcggtgc tatgttcact aaatcgcgtt tccacaattg ttatttttcg
      781 aaatgcatca gaacgagagc cgttagaaat tgcgccgcaa gcggatgctg atagccttca
      841 gcgtggtggc ggcaccgcaa tcgaatactc tcgtgccatc gagccggtag gccgcgaata
      901 cattcttaaa cattaccgtg cttacggcgg gcccgagcca gcagcaatcg atcaccaagg
      961 aattaatgat tcgtttgtgg gaaaagcatc tgttgtacgt tacttccatg caggtaaatg
     1021 gcttgagttg acaggagccg actaaaaggt cgcatgcaag gcgcttccgc gacccccgca
     1081 ccagcccaat gcgattaacg tatgtcttac ggttttgaca gcggcaacgg ttgatccatg
     1141 tctatctctg acttcaccgc caccacgcct gatgcattcg ccgaatttgc gctcggcctg
     1201 gtcagataaa ttagaaacgg tgccacctca aagggcatct tctcgaattc cgcgatggcg
     1261 ccttcgggtt cgaccttacg catcaccagg cggtcgtccc acggctggac gatgatgcgt
     1321 ccgccgagcg gcaaggctgc aagaacgttc gaggctgatt tgctatccgg ctgccgggaa
     1381 atcgccgacg aagcgattgt cgaactgttg ggaatgctat cgcccagatg cgggctgatc
     1441 aggttgggta tttccttggc gcgctcctgc aacgc
//
LOCUS       DATCBB010000545         1041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_148049, whole genome shotgun sequence.
ACCESSION   DATCBB010000545 DATCBB010000000
VERSION     DATCBB010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148049"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VII37_06010"
     CDS             complement(<1..713)
                     /locus_tag="VII37_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY5069318.1"
                     /translation="MMRQIAVIICFNASLCSAQSFRGADIPWTTYEAEAMTNTGTTLG
                     PKYDPYLVETESSGQKCVKLAATGQFVKFIAQSSANAIVVRYSLPDAPGGGGLNSTLS
                     LYQNDKFIQKLPVTSRYSWLYGKYPFTNNPKAGRPRNFYDEVRLKGLSIAGGDVLRLQ
                     KDNDATAAYCIIDLVDLENVAPPLTAPPDSLSVSDFGANGNGETDDTSALRNCVAVAK
                     KQGKSVWVGAGTFKITDDI"
BASE COUNT          225 a          279 c          293 g          244 t
ORIGIN      
        1 ttgatgtcgt cggtgatttt gaaagtgccg gcaccaaccc agacactttt tccctgcttc
       61 tttgccactg cgacgcaatt tctcaaagca gaagtgtcgt cagtctcgcc atttccgttc
      121 gcgccaaagt cgcttacaga aagcgaatcc ggcggcgcgg tcagtggtgg cgcgacgttc
      181 tccaaatcaa caaggtcaat gatgcaatac gcggcggtgg cgtcattgtc cttttgcaaa
      241 cgcagcacgt cgccgccggc gatggacaaa cctttcaacc gaacttcgtc atagaaattt
      301 cgcggccggc cagctttggg gttgttggtg aatggatatt tgccgtagag ccacgaatac
      361 ctggacgtga ccggaagttt ttggatgaac ttgtcattct ggtagaggct gagggtggag
      421 ttgaggccac ccccgcctgg cgcgtccggc aaactgtagc gcacgacaat ggcattcgcg
      481 gatgattgcg cgatgaactt aacaaattgt ccggtggcgg cgagcttcac gcatttctgc
      541 ccggacgatt ccgtctcgac gaggtaagga tcatatttcg gccccagcgt cgttcccgtg
      601 ttcgtcatgg cttccgcttc gtaagtagtc cagggaatgt ccgcgccgcg aaacgattgg
      661 gcagagcaca gcgaggcatt gaagcatata atgacagcaa tctggcgcat cataatagcg
      721 gcgcgtagtg tttgccgccc attttccagc ggcctttgac tgatgataga aatctgtttt
      781 tgacacggac gcagttcatt ttgggcaccg tgaagacacc ctctccgtcc tgcaaggaaa
      841 aaatggaatg caattttggc gcatcatcac gaagtcgccg gtgaaaaatc ataaaaccct
      901 ttcaatttga agctttgggc atcagcggct acgctgggcg gcgatgctta acccccaatt
      961 catgcgacac gccctccggc ttgcccggcg cggctagggc gcgacttcgc cgaatccggt
     1021 ggtcggcgcg gtgctggtga a
//
LOCUS       DATCBB010000546         1152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_116018, whole genome shotgun sequence.
ACCESSION   DATCBB010000546 DATCBB010000000
VERSION     DATCBB010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116018"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VII37_06015"
     CDS             complement(<1..489)
                     /locus_tag="VII37_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069319.1"
                     /translation="MAAHGSRNFAVALVLILAFGTCLQAKPKPQQRWTENAANDWYAR
                     QPWLVGSNYIPATAINQLEMWQADTFDHKRIDLELGWAESIGLNTMRVFLHDLLWQQD
                     AEGFRARIDTFLKIANKHHIKPVFVLFDSCWDPDPHLGKQHEPRPGVHNSGWVQSPGA
                     KSL"
     gene            complement(587..>1152)
                     /locus_tag="VII37_06020"
     CDS             complement(587..>1152)
                     /locus_tag="VII37_06020"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069320.1"
                     /translation="PKVHLVICNTMVRHELGRSEYNIRRGQCEEGVHQLSKALPGIRA
                     LRDVSLADLEEHRELLSAIVYQRCRHVVTENARTHWAAAAFESGEIATLRQLMTDSHR
                     SLRDDYEVSCRELDIMVELAMQQRGVLGARMTGGGFGGCTINLVQAAESDRFCDRVAE
                     GYQAAIGRRPDIYVCEASNGAGIAEPG"
BASE COUNT          254 a          332 c          311 g          255 t
ORIGIN      
        1 caaggatttg gcgcccgggc tttgcaccca accggaattg tgaacgccag gacgcggctc
       61 gtgttgtttt ccgaggtgcg gatcgggatc ccagcaagaa tcaaataaaa caaaaaccgg
      121 cttgatgtgg tgtttattgg caattttcaa aaaagtgtcg atccgtgccc taaagccctc
      181 tgcatcctgt tgccacagga gatcgtgcag aaaaacgcgc atggtgttga ggccaatgga
      241 ttcggcccac cctagctcga gatcgattcg cttgtggtca aacgtgtccg cctgccacat
      301 ctcaagttgg ttgatcgccg tagcaggaat gtagttgctg ccaacgagcc agggctgcct
      361 ggcataccaa tcgttcgcag cattttcagt ccaacgctgt tggggcttgg gcttagcctg
      421 aagacaagtg ccgaaagcga gaatcaaaac taaagcgacc gcgaagtttc gtgatccatg
      481 cgccgccatt tttctctaac ccccttagca cagttggcaa gactcggaca aacgaacgaa
      541 tgaatgcatc gcgctgcgcc tttgcgcgct taatgctgat atccagctag cctggttccg
      601 cgattcccgc tccgttggac gcctcacaca cgtagatatc tgggcgccgc ccgattgcag
      661 cttggtaacc ttcagccaca cggtcacaaa atcgatccga ttcagcggct tgcaccaggt
      721 tgatcgtaca tccgccaaat ccgccaccgg tcatccgcgc tccgagcacg ccccgctgtt
      781 gcatcgcgag ctccaccatg atgtcgagct cgcggcaact gacttcatag tcgtcacgga
      841 gactgcggtg cgaatcggtc atcaactggc gcaaagtcgc gatttcgccg gactcaaaag
      901 cagcggctgc ccaatgcgtg cgcgcatttt cggtcacgac gtggcggcaa cgttggtaga
      961 cgatggcgct gagaagctcg cgatgctctt cgagatcggc aagtgacacg tctcgtaagg
     1021 cgcgaattcc aggtaaagcc ttggacagtt gatggacccc ctcttcgcat tggccccggc
     1081 ggatattgta ttcgctcctc ccaagctcgt ggcgcaccat ggtgttacag attaccaggt
     1141 gcactttagg tg
//
LOCUS       DATCBB010000547         1017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_18163, whole genome shotgun sequence.
ACCESSION   DATCBB010000547 DATCBB010000000
VERSION     DATCBB010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18163"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VII37_06025"
     CDS             complement(<1..989)
                     /locus_tag="VII37_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5069321.1"
                     /translation="MQDGRVCGIYVIAEAEQRRQIAASTVLLATGGMGQLYRNTTNPA
                     VATGDGVAMAFRAGAEISDMEFVQFHPTALYLKNAPRFLLSEALRGEGAYLRNAELDR
                     FMPKYHPLGELAPRDIVARAIVHELEISGAKDPVAYLDLTHLDSQHGKTRFPRIYATC
                     LKYNVDITTELIPVRPAAHYAMGGVWTNLDGRTTLPGLYAAGEAAATGVHGANRLASN
                     SLLEGLVFGARAGRSMREELRSMRGKPEPERRTDSGLADAQAEEVIQAIQHLMWKDAG
                     IVRTGDGLRNAIQRLEELSSRVINPQTRRGHEAQNLLIAGTLVARSALAREESR"
BASE COUNT          219 a          294 c          279 g          225 t
ORIGIN      
        1 cggctttctt cccgagccag cgccgaacgc gccaccagcg taccggcgat cagcagattt
       61 tgtgcttcat ggccacgcct ggtctgcggg ttgatcacac gggagcttag ttcctccagg
      121 cgttgaatcg cgtttctaag cccatcgccg gtgcgaacta tgcccgcgtc cttccacatc
      181 aagtgctgaa tggcctgaat tacttcctcc gcctgagcat cggcgaggcc gctgtcggtt
      241 cgtcgttctg gttcaggctt cccacgcatg gagcgcaatt cttcccgcat actacgtccg
      301 gctctcgcgc cgaagaccaa gccctcaagc agggaattgc tggccaggcg gttagctcca
      361 tgcacgcccg tagctgcggc ctcacccgca gcatacaaac cgggcaatgt ggtgcggcca
      421 tcgagattgg tccatacgcc acccatcgca taatgcgcgg caggacgtac cggaatgagt
      481 tcagtagtga tgtcaacgtt gtatttcaag caggtggcgt atatccgggg aaaacgcgtc
      541 ttcccatgtt gcgaatcaag gtgagtcaga tctaagtacg caacaggatc cttagcgcca
      601 ctgatttcca gttcgtgaac gattgcgcgg gctactatat cgcggggcgc cagttcaccg
      661 aggggatgat atttaggcat gaagcgatca agctctgcat ttcgcaggta cgctccctct
      721 ccgcgaagag cttcggacaa caaaaatcga ggagcattct tgagataaag agcggtggga
      781 tggaactgaa caaattccat atcgctaatt tccgctccgg cgcgaaaagc catggcaaca
      841 ccgtctcccg tagcgaccgc gggattagtg gtatttcgat aaagttggcc catgccgccg
      901 gtcgctaata acacggtcga tgctgcaatc tggcggcgtt gttccgcttc ggcgattacg
      961 tagatgccgc acacccttcc atcctgcaag cgtaagtcgg tggaaaattc gaattcg
//
LOCUS       DATCBB010000548         1140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_472286, whole genome shotgun sequence.
ACCESSION   DATCBB010000548 DATCBB010000000
VERSION     DATCBB010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472286"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VII37_06030"
     CDS             complement(<1..235)
                     /locus_tag="VII37_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069322.1"
                     /translation="MTKVGFVSLGCPKNLVDSEVMMGILARGGYELTPRADEAEVLVV
                     NTCSFIEAAQKESVDAILEMAEHKKFGAAKKLII"
     gene            complement(324..>1140)
                     /locus_tag="VII37_06035"
     CDS             complement(324..>1140)
                     /locus_tag="VII37_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY5069323.1"
                     /translation="ETRAGHCEYFASSMTVMLRTLGIPAREVNGFLPGEYNDLAGDYV
                     VRASDAHSWVEVFFPGSGWVTFDPTPAGPDVAGGILSRVEQYLDWLSLTWNEWVISYD
                     FAHQVVLAQSLKTNSRNWSDSFRSWFDKKQQQARSRMKDWEFQHSSLRYLLPVALVFF
                     LVILRADMIPELVRRLKVYAQMRVGRSAHTNPQLAARLYSELLHVLNKRGISRLETQT
                     PLEFAAAVGDARVAPAVREFIDIYSQARFGEAPCNAVRLRELMSQVRQSFRTR"
BASE COUNT          228 a          287 c          358 g          267 t
ORIGIN      
        1 cgataattaa ttttttggca gcgccaaatt ttttgtgctc ggccatctcc aggatggcgt
       61 ctacggattc cttttgtgcg gcttcgatga agctgcaggt gttgacgacg aggacttcgg
      121 cttcatcggc gcggggggtg agttcgtaac cgccgcgggc gagaatgccc atcatgacct
      181 cgctgtcgac gaggtttttg gggcatccga ggctgacgaa tccgacttta gtcatagcta
      241 tgatttgcgg ggtttgttgc ggtgtttacg cgctttttat tgtaacacgc gcgggtcttt
      301 cggttggctt tcgctgattt ggctcagcgc gtgcgaaatg actggcgtac ttgggacatc
      361 agttctctta ggcgcacggc gttgcagggc gcttcgccga aacgagcctg gctgtaaata
      421 tcgatgaact ctcgcacggc gggcgcgacg cgtgcgtcgc ctacggcggc ggcgaactcg
      481 agtggggttt gcgtttcgag gcgcgagatt ccgcgtttat tcaggacgtg cagcaattcc
      541 gagtacaaac gggcggccaa ctgtgggttg gtatgcgcgg agcgacctac gcgcatttgg
      601 gcatagacct tcaggcggcg taccagttcg gggatcatgt cggcgcgtaa aatcaccagg
      661 aaaaatacca gggcgacggg caataagtag cgcaggctgg aatgttggaa ctcccaatct
      721 ttcattcggc tccgtgcctg ctgttgcttt ttatcaaacc aggaacgaaa cgagtcgctc
      781 cagttgcgtg agttggtttt taggctttga gctagcacta cttgatgcgc gaagtcgtaa
      841 ctgatgaccc attcgttcca ggtcagcgag agccagtcga ggtattgctc gacgcgagag
      901 agaatgcctc cggcaacatc aggtccggcc ggtgtgggat cgaaagtgac ccagccactg
      961 ccggggaaga aaacttctac ccagctatgt gcgtcgctgg cgcgcactac ataatcgccg
     1021 gcgagatcgt tgtattcgcc gggcagaaag ccattgacct cgcgcgcggg gatccccaag
     1081 gtgcgcagca taacggtcat ggaggaggca aaatattcgc agtggccagc gcgagtctca
//
LOCUS       DATCBB010000549         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_116182, whole genome shotgun sequence.
ACCESSION   DATCBB010000549 DATCBB010000000
VERSION     DATCBB010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116182"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1240
                     /locus_tag="VII37_06040"
     CDS             116..1240
                     /locus_tag="VII37_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069324.1"
                     /translation="MPLDQLDQQMVDSLPPLADVYDRYGVQVNALSVPEIFALYERTG
                     FLYPDKAARLFPHLAQVRENWRRMLKVGDSLLYVLTAGDSKKGLASLAVWRASQNGWM
                     SQHLVSDNNPYASRAVLLAAAAARIRKDCDVSAQNWFRPENRFPARVFGSMVKTIGES
                     LSSVQRHAYFGLPRTLALPSVKRIRVVPYNPSHKAALLEIASQARGRIYVIAEELEHD
                     TEFETLADLYRSVGLRRSRHVWLAYRAHKEEPIGAVIAYRGPLGINFSYLENRCDLLL
                     QPTLPEAEVSDVAAALLSASAVAYKDFELDEIPVIADEMATHALMKLGGEFVRHYCQG
                     IWLKDGHPGFYKHVDGFYSRILARLERQQEPQESLIGQER"
     gene            1252..2052
                     /locus_tag="VII37_06045"
     CDS             1252..2052
                     /locus_tag="VII37_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5069325.1"
                     /translation="MSAVDYIMEDPREAKRLEAKVDAYAWVQKYVAHRVGAGAEVLSV
                     GCGPGVILREMVGLGKSIKATGLDVSADRIQEAKERHRGVEAMSFVRGDAHAMEFPSN
                     RFDLVYSRMLIEYLKDKQQAVREMVRVCRPGGTVLLQDLDGQLLWHYPEDVELQRNSE
                     KVVNALAATGFDPFVGRKLFSFAQKAGLPHIDVQVESYHLIAGVVGPEILKQWELKLE
                     IAAPQLTKVLGSERAAKEHAQRFLEYLRRPDTLTYSTVFTVSGKKPLQ"
     gene            2263..>2449
                     /locus_tag="VII37_06050"
     CDS             2263..>2449
                     /locus_tag="VII37_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069326.1"
                     /translation="MTVFLAANGAAQSQQSVPDVTALSMEDLMNIQVTSVSKRTQKVA
                     DAAAAIFVITQEDLRRSG"
BASE COUNT          570 a          624 c          728 g          527 t
ORIGIN      
        1 cacctaaaaa cgtactgata gtactagcta cgtccctcgt gtggccgtcg gctaggtttc
       61 gtagatgcgt ttacgcgcag tgtgaccagg tggccggatt ggccacggag aagaaatgcc
      121 gctagatcaa ttagatcagc aaatggtcga cagccttccg ccgctggcgg acgtgtatga
      181 ccgttatggc gtgcaggtca acgcgctgtc ggttccggaa atattcgctc tttatgagcg
      241 caccgggttc ctatatcccg ataaagctgc ccggcttttc cctcatcttg cgcaagtgcg
      301 tgaaaactgg cggcgaatgt taaaagtcgg cgactcgctt ttgtatgttc tgactgccgg
      361 cgacagcaaa aaaggactgg catctttagc ggtttggcga gcctcgcaga acggctggat
      421 gtcccagcat ttggtttccg acaacaatcc ctacgcatct cgcgcggtat tgctcgcagc
      481 ggctgcggcc agaattcgca aggattgcga cgtatcggcg cagaactggt ttcgcccgga
      541 gaatcgcttc ccggcgcgcg tttttggctc gatggtaaag accattgggg agtcactttc
      601 gtcagtgcag cggcatgctt acttcgggct accgcgaaca ctggcgctgc cgtccgtgaa
      661 acgcattcgc gtagtgccct acaacccatc gcacaaggct gccttgctcg aaattgcttc
      721 tcaggctcgg ggcagaatct atgtcatcgc tgaggaatta gaacacgaca cagagttcga
      781 aacattagca gacttgtatc gcagcgtcgg attacgtcgt tcgcgacacg tttggctagc
      841 gtatcgcgca cacaaagaag aacccatcgg cgcggtcatc gcgtaccgcg ggccgttggg
      901 cattaatttc agctacctcg aaaaccgttg cgacctgcta ctgcagccca cgcttccgga
      961 agcggaagtc tccgatgtcg ccgccgcgct tctaagcgcc tccgccgttg cttacaaaga
     1021 tttcgaactg gatgagattc cggtgatcgc ggacgaaatg gctacccacg cactgatgaa
     1081 actgggaggc gagtttgtcc gccactactg ccagggaatt tggctcaagg acgggcatcc
     1141 gggtttctac aagcacgttg acggtttcta ttcacgaata ttggcgcgac tcgaaaggca
     1201 gcaggagccg caggaatctc tgattgggca ggaacggtaa tgagtaaggg cgtgtccgca
     1261 gtcgattaca tcatggaaga tccacgcgag gcgaagcgcc ttgaggcgaa agtagacgcc
     1321 tacgcctggg tacaaaagta tgtggcgcat cgcgttggcg cgggcgcaga agttctttcg
     1381 gtgggttgcg ggccgggagt gatcttgcgt gagatggtcg ggctgggcaa atccataaaa
     1441 gcgactggac tggatgttag cgcggaccgc atacaggaag ccaaagagcg gcatcgcggg
     1501 gtggaagcca tgagttttgt gcgtggagac gcgcacgcca tggaatttcc atcgaatcgc
     1561 tttgacctgg tgtactcgcg gatgttgatc gaatacctga aggacaaaca acaagcggta
     1621 cgcgaaatgg ttagagtttg ccgcccgggt ggtacggtgc tgttgcaaga cctggatggg
     1681 cagcttttgt ggcattaccc ggaagatgtg gagttgcaac gtaacagcga gaaagtcgtt
     1741 aacgcgctgg ctgccaccgg atttgatccc ttcgtgggcc gcaagctttt ttcattcgcg
     1801 caaaaagcgg ggctgccaca catcgatgtg caagttgaaa gctaccactt gatagcagga
     1861 gtagttggtc ccgaaatttt gaagcagtgg gaactcaagt tagagatcgc agcaccgcaa
     1921 ctgacgaagg tgttgggcag cgagagggca gcgaaggagc acgcacaaag atttcttgaa
     1981 tatcttcgcc ggccggacac gctgacgtac tcgacagtat tcacggtaag cggaaaaaag
     2041 ccgctgcagt aagggcgtaa gcgtttcgca acttcaggta tcagcgggca aagcgttgaa
     2101 atgcagggaa ggaattcagg ccaggaaagc gcaaatgaat tttggggatc caaaacgatt
     2161 gcttttcaag atttcagaga agggaatata accatgtttt cgaatcgccc acccagttta
     2221 gccgccgcac ggttcgtcgg gcgaaccatg ctagcgagtt tcatgactgt ctttttggca
     2281 gcgaacggcg ctgcgcagag ccagcagagc gtgccggacg ttaccgctct cagcatggaa
     2341 gacctcatga acatacaggt aacctccgtc tccaaacgga cgcagaaggt cgccgatgcc
     2401 gccgcggcaa ttttcgtcat cactcaggag gatttgcgac gctcaggcg
//
LOCUS       DATCBB010000550         1200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_407900, whole genome shotgun sequence.
ACCESSION   DATCBB010000550 DATCBB010000000
VERSION     DATCBB010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407900"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          262 a          341 c          334 g          263 t
ORIGIN      
        1 atagaagacc cataggacgc tatggaatgg ctggcgcgcg tggtccacga gaccatccgc
       61 aggccaaatc gttccctggc ttggtgcggc cagtagttgc atgtgttgcc agtgccaaaa
      121 cactacagcg agggcagcga gtcctcgcag cgcatcaaga gagtgcaatc gtattttgct
      181 ttctgacatc gacggaccta agcggaggtt attcgaggat taaatggcgt cgcggcgcac
      241 gcttaggcga taagctccgc ctcgccgaga accgccacgt tgcgtgtgag ttacaatgat
      301 gccatggggc cggaaccgat accgaggcgc attttacggt tgtcggatga ccaaggatgc
      361 atgactgcgc tcgagacggg tgtcacgctg ggtgctcgcg ggctacgctg cttgtgtcga
      421 ttgcgcagat gatgtcgggc gtgcggtcgc cgcggccaac ggcacggcat gagtatcgcc
      481 gtggtccccg caactgccga ggtctgtagt ttctgacaag agtgcgtgcc cgcttccgac
      541 agattatttt ttatcttaca cgcaccatcc gacctcgata agagttttgg acagcttttt
      601 ttcttggagc ggtcatgcaa cctttatatc ttgcggtcgg tgcggcgctt tatctcggct
      661 ttgctgtctg gtgcatcctg gcaccgcaaa actgatagca cctggaacgg gtcgaggttt
      721 cgagccattg acgcaatagg cgcgcggcag cgccgcactg catgctggcg cagagagcgc
      781 tgccaatcac ttttactgaa acgtactgac tggctgcaaa gactgttgcg agccgcccgc
      841 acccgaacgc aaactcccat ccgcgagaaa cgcctgtgag agggtctcct atcacttatc
      901 ttgaagtgtc ccacccactg acccgaaaaa ttgagtcgcg gccggcactc acccgtcaag
      961 ctggaaaaaa ttggctgatt ataggtgcac aattgaaacg catgcccacc cgaggtccac
     1021 ttcccggccg acgccgataa tccagccaag ccaaaacaca tccgcgcgtc gaaattgata
     1081 gcacaacccc tacttcgttc actccctgtc tatcggcata gccggcttct aaccacttga
     1141 aactactgga atctgccacc attgagacct gacgattggc aatgtaggtg ggtttgcgag
//
LOCUS       DATCBB010000551         1087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_342395, whole genome shotgun sequence.
ACCESSION   DATCBB010000551 DATCBB010000000
VERSION     DATCBB010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342395"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VII37_06055"
     CDS             complement(<1..328)
                     /locus_tag="VII37_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069327.1"
                     /translation="MTDLLEWRSEFPILENSTYMISNSLGAMPRAVYESMRAYADSWG
                     NRGIRAWEEGWWEMAVGVGDKIAPLIGAGSGEISLHQNVTITQAVISSCFDFGSKRNK
                     IVMVEQE"
     gene            complement(357..>1087)
                     /locus_tag="VII37_06060"
     CDS             complement(357..>1087)
                     /locus_tag="VII37_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5069328.1"
                     /translation="GLEIVGATAGEAANPEIEVPRALRRTLSFLVLFYLGGLAIVVGI
                     VPWNELGLGESPFVRVFRSVGIPGAGNVMNFVVLTAAVSSAMCNLYFTARLLFSLARG
                     GYAPASLGKLSKRGMPVVAVMASGFGMVIALVLSRYFEQTAFVFMIGVAFFGGPFIWI
                     MILVTHLAFRRANERTGNAVMRFAPPGPWSSLFGLAALLAVLISTWWVPGFRITMAAG
                     AIWLVFITVCYVVWSRVARERANT"
BASE COUNT          265 a          399 c          236 g          187 t
ORIGIN      
        1 attcctgctc caccatcaca attttgtttc gcttactgcc aaaatcgaaa cacgagctga
       61 tcaccgcctg cgtaatcgtc acgttctggt gcagcgatat ctctccggag ccagccccga
      121 tcagcggcgc gatcttgtcg cccacgccca ctgccatttc ccaccagcct tcttcccaag
      181 cgcgtatccc acgattgccc cacgaatccg catacgcgcg catgctctcg taaaccgcgc
      241 gcggcatcgc gcccagcgaa ttgctgatca tgtacgtgga gttttccaga atcggaaatt
      301 cactgcgcca ttcaagaagg tcagtcatgg gagttccaca ttaaccgcat tttacgttag
      361 gtattcgccc tttcgcgtgc cacccgcgac cacaccacgt agcacaccgt gataaacact
      421 agccagatcg cacccgccgc catggtgatt cgaaatcccg gcacccacca agtcgaaatc
      481 aacaccgcca acagcgccgc cagcccgaaa agggaactcc acggcccagg cggcgcgaac
      541 cgcatcaccg cgttgcccgt gcgctcattc gcccgccgga acgccaggtg cgtcaccaga
      601 atcatgatcc agatgaacgg cccgccaaaa aacgccactc cgatcatgaa cacaaacgcg
      661 gtctgctcga aatagcgaga aagaaccagc gcgatgacca tcccaaatcc cgacgccatc
      721 accgcgacca ccggcatccc gcgcttgctc aattttccca acgatgccgg cgcatatccg
      781 ccgcgtgcca gcgaaaaaag cagtcgcgcc gtgaaataca aattgcacat cgcgcttgaa
      841 accgccgccg tcagcaccac gaaattcatc acgttgcccg cgccgggaat tcccactgac
      901 cggaacaccc gcacgaaagg actttccccc agtcccagtt cattccacgg aacaatcccc
      961 actacgatcg ccagcccgcc taggtaaaaa agcaccagaa aagacaacgt ccgccgcaac
     1021 gcgcgcggca cctcgatttc cggattcgct gcttctcccg ccgtagctcc cacaatttcc
     1081 agtccca
//
LOCUS       DATCBB010000552         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_116338, whole genome shotgun sequence.
ACCESSION   DATCBB010000552 DATCBB010000000
VERSION     DATCBB010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116338"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VII37_06065"
     CDS             complement(<1..1813)
                     /locus_tag="VII37_06065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5069329.1"
                     /translation="MPSAISTAANGLKKMFAPVSPFFILLFLVPPLITLAAASPAAPT
                     ATVKRQAGAQFQRAEQQRATLNLKSITKRTLGDYRQVVSSYRRVYLITPRANEVPDAL
                     IAIAQLYTEMGERFGRNYYQNAVDSYRFLLREYSASRHAPDAMLVCAKLQRDQLADYA
                     GATQTYKDFLKRFPRSPRKREAQEGVAELALLENSAKPSPDKDKATSNASAEAVALPP
                     DESSDALPPSPAASHSLGIPRLRRIRSAVTEDSTRVTIDLEDVVHYNSGRLENPERIF
                     FDLREARLSSDVAREVTRENTNVDGDVLTAIRVAQNQAGVVRIVLNVNRVKEYVASIS
                     QNPPQLLIDLYRKQPPPAILRIARASHNSSNIHETGDLRAAAAGGIESAVDGTSDTLV
                     RSPQTLKVSKTSKPDLDQPAIAPGPTHDGQSTLTRALGLKIGRIVVDAGHGGHDTGTI
                     GPTGLMEKDLCLDVALRLGKIIKQRLPGAEVVFTRSDDSFIPLEERTAIANEAHADLF
                     ISIHANSSQDHGARGIETYYLNLKGSPEAMEVAARENAVANVGIHDLEDLVKKITRNE
                     KIDESKEFAEDIQTSLSQRVQKANKTVKNRGVRKAPFV"
     gene            complement(1870..>2154)
                     /locus_tag="VII37_06070"
     CDS             complement(1870..>2154)
                     /locus_tag="VII37_06070"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5069330.1"
                     /translation="NPQQNIDGGVHYLKTLLDRYNGNLDMALAAYNAGEGAVDRAHGI
                     PAFRETRSYVQKVQNAYFQPGSGRLTDTWVRPHPIRRETDSNGRIIFNND"
BASE COUNT          465 a          527 c          640 g          522 t
ORIGIN      
        1 cgacgaatgg agctttgcgg acgccacgat tcttgacggt tttattggct ttttgcacgc
       61 gttgggacag ggaagtttga atgtcctcgg caaattcctt ggattcgtca attttttcgt
      121 tacgggtgat ttttttgacg aggtcttcca gatcatgaat acctacattg gcgacggcat
      181 tttctcgcgc agccacttcc atggcttcgg gagagccttt taaattcaga taataagttt
      241 caataccgcg ggcgccgtga tcttggctgg aattcgcgtg tatggagata aaaaggtcag
      301 cgtgcgcttc attggcaatg gcggtgcgct cttccaacgg gataaaagaa tcgtcggaac
      361 gggtgaagac gacttctgcg ccggggaggc gctgcttgat aattttgccg aggcgcaggg
      421 cgacgtccag gcacaaatct ttttccatca agccggttgg accaatcgtt ccggtgtcat
      481 gtccgccgtg tccggcgtca accacgatac gtccgatttt taggccgagc gcgcgcgtca
      541 gcgtggattg gccgtcgtgg gtgggtcccg gggcgatcgc gggctgatca aggtcgggct
      601 tggaggtctt ggacactttg agcgtttgcg ggctacgaac caaagtgtca ctggtcccat
      661 caaccgcaga ttcaataccg cccgcggcag cggcgcgaag atcgccggtt tcatggatat
      721 tggaagaatt atgagaagcg cgcgcgattc tcagaattgc cggcggcggc tgctttctgt
      781 acagatcgat caaaagctgt ggaggattct gtgagattga ggctacatat tccttgacgc
      841 gatttacatt aaggacgatg cgcactacgc cagcctgatt ttgagcgaca cgaatcgcgg
      901 tgagcacgtc gccatctacg ttggtgtttt cgcgagtcac ctctcgtgcg acgtcagagc
      961 taaggcgcgc ttcccgcaaa tcaaaaaaaa tgcgctcggg attttccaag cgaccagagt
     1021 tgtaatgcac tacatcttcc aggtcgatgg ttacgcgagt ggagtcttcc gttacggcac
     1081 tgcgaatgcg gcgcagtcgc gggatgccga gtgagtggga agctgccggc gaaggcggaa
     1141 gcgcgtcgct ggattcatct ggaggaaggg caactgcttc ggcggaagcg ttggacgtgg
     1201 ccttatcttt gtcaggcgat ggcttggcac tattttcgag cagcgccaat tccgctacgc
     1261 cttcctgtgc ctcgcgcttt ctcggtgaac gcggaaagcg cttcaaaaaa tccttatacg
     1321 tctgagtagc gccggcataa tcggcaagct gatctcgttg cagttttgcg cagaccagca
     1381 tggcgtcagg tgcgtgacgg ctggcagaat attcgcgcaa cagaaaacgg taggagtcca
     1441 cggcgttttg atagtagttg cggccaaagc gttcgcccat ttccgtatag agctgagcaa
     1501 tggcaatcaa agcgtcaggc acttcgttgg cgcgcggagt gatgagataa acgcggcgat
     1561 agctggagac tacctgacga taatcgccga gcgtgcgctt ggttatagat ttgagattta
     1621 gcgtggcccg ctgttgctcc gctctctgga attgggcgcc agcttgacgt tttacagtgg
     1681 cggttggcgc ggcaggcgat gcggcggcaa gagtgatcag cggaggaaca aggaacaaca
     1741 agatgaaaaa tggcgaaacg ggggcgaaca tcttttttaa accatttgcg gcggttgaaa
     1801 tcgcgctcgg cattttgttt tcgaagtcgt tcgcaagcga caaactatgc tgttacaaga
     1861 ctcctagttt caatcattgt tgaagataat tctaccgtta gaatcggtct ctcgtcgaat
     1921 aggatgcggt cggacccaag tatccgtgag acgtcccgag cccggctgaa agtaagcatt
     1981 ctgcactttc tggacgtaac tccgtgtctc gcgaaaggcc ggaataccat gagcccggtc
     2041 gacagcccct tcgcctgcat tgtacgccgc tagagccatg tccaggttgc cattgtagcg
     2101 gtccaacagc gtctttagat aatgaacacc gccgtcgata ttctgttgcg ggtt
//
LOCUS       DATCBB010000553         1630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_212767, whole genome shotgun sequence.
ACCESSION   DATCBB010000553 DATCBB010000000
VERSION     DATCBB010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212767"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VII37_06075"
     CDS             complement(<1..629)
                     /locus_tag="VII37_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY5069331.1"
                     /translation="MKWLGRAAILLLACIAATPLLTAQNPRGSLRGVVQDASGGRVSG
                     ATIIVTSADFSTERQTKSSDRGEFRVDDLLPGEYRVCVEATAFAAAESRVEVQVSSGR
                     DINVTLKPAGVHQEVNVSAEASSITTQPVDVASAVQQGIVTAQDLREIPLAHRSFANI
                     AYLVPGTEPVEPSDPTKARITAVSTGGSSGLNNELSVDGVDNSDDYIGG"
     gene            complement(700..>1630)
                     /locus_tag="VII37_06080"
     CDS             complement(700..>1630)
                     /locus_tag="VII37_06080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5069332.1"
                     /translation="AGGFSPTISLAKDGATMAFVRESYAAPPEVWAGKVGEWKQITRR
                     NEGVTREWGDAKSIEWKNGGHEVQGWLLYPKDFDASKKYPLVVNVHGGPSWASVAKWP
                     SPHGYASALAGAGYFVLSPNPRGSYGQGEAFTRANVKDFGEGDFADILAGVDEAMRVA
                     PIDGNRLGLTGWSYGGFMTMFGVTQSNRFKAAMAGAGIANWESYYGENLIDQWMIPFF
                     GKSVYDDPEIYAKSSAINFIKKVKTPTLVIVGDSDGECPAPQSYEFWHALKAQAVETQ
                     LVVYEHEGHLFAKPQHQRDVIERTLAWFDAHLK"
BASE COUNT          339 a          584 c          347 g          360 t
ORIGIN      
        1 aatccgccga tgtaatcgtc ggaattatcc acgccatcca ccgacaactc gttattcaaa
       61 cccgaacttc ccccagtgga caccgccgta atccgcgcct tggtaggatc cgaaggctct
      121 accggttccg tccccggcac caggtacgca atatttgcaa agctgcgatg cgccagcggg
      181 atttcgcgca agtcctgcgc cgtcacgata ccttgttgta ccgcgctcgc tacatccacc
      241 ggctgcgtcg tgatcgacga cgcttccgcg ctcacattta cttcctggtg tacgcctgcc
      301 ggtttcagcg tcacatttat atcgcgcccc gaactcacct gcacttctac acgcgattcc
      361 gccgccgcga acgcggtcgc ctccacacac acgcgatatt cgcccggcaa taaatcatcc
      421 acgcgaaact cgccacggtc gctggacttt gtttgccgtt ccgtcgaaaa gtccgccgac
      481 gtgacaataa tcgtcgcgcc actaacccgc cccccgctgg catcctgcac cacgccccgc
      541 aaacttccac gcggattctg ggccgtcaat aacggcgtcg cggcaatgca tgccaataac
      601 aaaatagccg cacggccaag ccacttcatc cctcacctct tgctgctttc ggagcttgat
      661 agcctagaga attgccgtcc ctttttccag ccacagtaat tatttcagat gcgcgtcgaa
      721 ccacgccagt gtgcgctcga tcacgtcccg ttggtgctgc ggtttcgcga acaagtgccc
      781 ttcatgttca tagaccacca gttgcgtctc cactgcctgg gcctttaatg cgtgccagaa
      841 ttcgtacgac tgcggcgcag ggcattcccc atcgctatcg cccacaataa ctaacgtcgg
      901 ggtcttcact ttcttgatga aatttatcgc cgaactcttc gcataaattt ccggatcgtc
      961 gtacaccgac ttcccaaaaa agggaatcat ccactgatcg attaaatttt ctccgtagta
     1021 gctttcccag ttcgcaatgc ccgcgccagc catcgccgcc ttgaaacggt tcgactgcgt
     1081 caccccgaac atggtcataa atccgccgta gctccaacca gtcagcccca gccggttccc
     1141 atcaatggga gccacgcgca tcgcttcatc cacgcccgcc agaatgtcgg caaaatctcc
     1201 ctcgccgaaa tccttcacgt tcgcgcgcgt gaacgcttcg ccttgtccat aacttccccg
     1261 cggatttgga ctcagcacaa agtatccagc tcccgccagc gcgctggcat agccatgcgg
     1321 cgaaggccac ttcgcaaccg aagcccaact cggcccgcca tgcacattaa ccaccagcgg
     1381 atattttttg ctggcatcaa aatccttcgg atacagcagc cacccttgta cttcatgccc
     1441 accgtttttc cattcaatac tcttcgcatc tccccactcg cgcgtaacgc cctcattccg
     1501 ccgcgtaatc tgcttccact cgcccacttt ccccgcccat acttccggtg gcgccgcata
     1561 cgattcccgc acaaacgcca tcgtcgcgcc atctttggcc agcgaaatcg tcggcgaaaa
     1621 tcctcccgcg
//
LOCUS       DATCBB010000554         1178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_342481, whole genome shotgun sequence.
ACCESSION   DATCBB010000554 DATCBB010000000
VERSION     DATCBB010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342481"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1178
                     /locus_tag="VII37_06085"
     CDS             <1..>1178
                     /locus_tag="VII37_06085"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEY5069333.1"
                     /translation="DVLENFWREGIQRNKNFDSIITLGLRGANDTPMAKGGPETNMAL
                     LEKIVEVQRKIIAEEVNSNLTVVPQLWCLYKEVLDFYNAGMRAPDDVTLLWAEDNWGN
                     VRRLPTAGERKRGGGAGVYYHFDYHGGPRNYQWLNTSPIAKIWDQLSLAKQYGADRIW
                     IVNVGHFKGYEFPLEYFMNLAWDTDRWTNDNLDEFTRLWAEREFGPEHAGDIADILSK
                     YTKYNGRRKPEMLAPDTYSLVNYQEAEDVVADFKAITGRAEAIYQELPDDRRDAFYEL
                     VLFPTKASALVNELYLAAGKNALFTRQGRASASDFATETRTLFQADTNLMAYFNLTFA
                     HGKWNHFMDQTHLGYTTWQDPPHNSLRAIKLTEIDVPDAAAMGVAVEGSEAAWPNTNG
                     "
BASE COUNT          277 a          317 c          352 g          232 t
ORIGIN      
        1 gatgtcctcg aaaacttttg gcgcgaaggc atccagcgga acaagaattt tgacagcatc
       61 atcaccctcg gtttgcgcgg cgcgaatgac acaccgatgg ccaaaggcgg gccggaaacg
      121 aacatggcct tgctggaaaa aatcgtggag gttcagcgga aaatcatcgc cgaagaagtg
      181 aattccaatc taaccgtagt gccgcagctc tggtgcctct acaaggaggt tttggatttc
      241 tacaacgccg ggatgcgtgc gccggacgat gtgacgttgc tgtgggcgga ggataattgg
      301 ggcaacgtcc gccggctgcc gacggcgggt gaacgcaagc gcggcggcgg cgcaggcgtt
      361 tattatcatt tcgattacca cggcgggccg cgaaattatc agtggctcaa caccagtccg
      421 atcgccaaaa tctgggatca gctgtcgctc gccaagcaat atggcgcgga ccggatttgg
      481 atcgtgaacg tcggtcattt caagggctat gaattcccgc tggaatattt tatgaacctt
      541 gcctgggaca cggatcgttg gaccaacgac aatcttgacg aattcacgcg gttgtgggcg
      601 gaacgcgaat ttggcccgga gcatgccggc gacatcgccg acattctttc caaatacacc
      661 aaatacaatg gccggcgcaa accggagatg ctcgctcccg acacttacag cctggtcaat
      721 tatcaggaag cggaagacgt ggtggcggat ttcaaggcca tcaccggccg cgcagaagcg
      781 atttaccaag aactgccgga cgaccggcgc gatgcgtttt acgaactggt tttgtttccc
      841 accaaggcca gcgcgctggt gaacgagttg tatctcgccg ccggcaaaaa tgcgctgttc
      901 accaggcagg gccgggcgag cgccagcgat tttgccaccg agacgcggac actttttcag
      961 gcggacacga acttaatggc ttatttcaac cttacgtttg cccatggcaa atggaatcac
     1021 ttcatggatc agacgcatct gggttacacc acctggcaag atccgccgca caacagcctg
     1081 cgcgcgatca agctcacgga aattgacgtg ccggacgcgg cggcgatggg cgtggcggtg
     1141 gaaggttccg aggctgcgtg gccgaataca aatggcga
//
LOCUS       DATCBB010000555         2041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_116479, whole genome shotgun sequence.
ACCESSION   DATCBB010000555 DATCBB010000000
VERSION     DATCBB010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116479"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VII37_06090"
     CDS             complement(<1..1451)
                     /locus_tag="VII37_06090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5069334.1"
                     /translation="MQRFAISILASFLATPLFAQFAPAPGTGPGLNETVEAIDSARVT
                     TRILYITAHPDDESAAVLTYLARGLHADVALLSLTRGEGGQNVLGPEQAPQLGLIRTQ
                     ELLAATRGYGVKLFFTSAKDFGFSKTPEETEKLWGDQVLADMVRVIRTFRPNIVINNF
                     GGVHSGHGHHQAAGVWTPQAVKLAADPSYGRAANAMATVASTNDSSTALETPWGDKDH
                     PVQILNVDRSGGSATNSASLQLPLDDVSPLWGKTWREIGIDAFANHRTQGISGVLGSP
                     FLHRPIALVSESGEKIDPAMFAQPLRALGGDAGENGCAPSSFFCPVLQHAEEDLAKAR
                     EDALSLHWPQSASAIAQAAREIASFYPGGGHAPHWIASDPRLADLHLAQRRVDRALAL
                     AAGLEIVAESDRSEVVVGEPFMVDAEAHCRKEAGCELGKLDLVLPAKANQTVSTTDPA
                     KTQKFTVLIEAALPPLTPWEQQQPEPPPLAIAK"
     gene            complement(1463..>2041)
                     /locus_tag="VII37_06095"
     CDS             complement(1463..>2041)
                     /locus_tag="VII37_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5069335.1"
                     /translation="AQNSPFLVSTFVETISGPFAARIATMLVLWIAFASIFASLLGYS
                     RIPYAAALDGSFFSIFARVHPQKKFPHVSLLFLGAVAFVSSLLFRLSTVIAAILATRL
                     LVQFIGQAIGLILLHRRWPPERFPFKMWLYPLPAILTMCGWAALFWATGAARKWGLLL
                     IGFGIVAFLLRAREVRQWPFAAAQARPEPEGA"
BASE COUNT          449 a          542 c          653 g          397 t
ORIGIN      
        1 tgtttggcaa tagcgagcgg cggcggttcc ggctgttgct gctcccacgg agttaatgga
       61 ggaagcgctg cctcgatgag tacggtaaat ttctgggttt ttgctgggtc cgtagtggag
      121 actgtttgat tggctttcgc gggcaacacg aggtccagtt tgcccagctc gcagccagct
      181 tcctttctgc aatgggcctc ggcgtctacc atgaatggct cgccgaccac gacttcactt
      241 ctatcggatt ccgcgacaat ctcgagtcca gcggccaagg ccagagcgcg atccacgcgg
      301 cgttgggcga gatgcagatc ggctaatcgc ggatcactag cgatccagtg tggggcgtga
      361 cctccgccgg gatagaacga agcgatttct ctggccgcct gggcgattgc cgaagccgat
      421 tgcggccagt gcagggataa tgcgtcttcg cgggctttcg ccaggtcttc ttccgcatgt
      481 tgtaggacag gacagaaaaa tgatgatggg gcgcagccgt tttcgccggc atcgccaccc
      541 aaggcgcgaa gcggttgagc gaacatggcg ggatcgattt tttctccgct ttcggagact
      601 aacgcgatgg ggcggtgaag aaagggcgag cccaagacac cggagatacc ctgggttcgg
      661 tgattcgcaa atgcgtcgat gccaatttca cgccacgttt ttccccacaa aggcgagacg
      721 tcgtctaagg gaagttgcag gctggccgag ttggtagcgc ttccgccgct acgatcgacg
      781 ttcagaattt gtacgggatg atctttgtcg ccccacggtg tttctaacgc tgtcgaggag
      841 tcgttcgttg atgcaacggt ggccatggca tttgccgccc gaccgtacga cggatcggct
      901 gccagcttta cggcctgagg agtccacacc ccggctgctt gatgatggcc gtggccggaa
      961 tgcacgccgc caaaattatt gatcacaatg ttcggtcgaa aagtgcggat tacacgcacc
     1021 atatccgcga ggacctgatc gccccacaac ttttctgtct cctcaggtgt tttcgagaag
     1081 ccaaaatctt tagcgctggt aaaaaaaagc tttacgccat agccacgggt cgcggcgagg
     1141 agttcttgcg ttcgaatcaa tccaagctgc ggggcctgtt cggggcccag tacgttctgg
     1201 ccgccttcgc cgcgagttag agaaagcaat gcgacatcgg cgtggaggcc gcgcgccagg
     1261 taggtcagta cagcggcgga ctcgtcgtca ggatgcgcgg tgatgtacaa aatgcgggtg
     1321 gtgacacggg cgctgtcgat ggcttcgacg gtttcgttca ggccagggcc ggtgccggga
     1381 gcgggcgcaa actgcgcgaa aaggggcgtt gcgagaaaag acgcgaggat ggaaattgcg
     1441 aatcgttgca atgctgaaag tctcaagcac cctccggctc ggggcgcgct tgggcggccg
     1501 cgaaaggcca ttgtcgcact tccctggcgc ggagcaaaaa tgccacgatt ccgaagccga
     1561 tcaggagcaa gccccatttg cgcgccgcgc cagtggccca gaagagcgcg gcccagccgc
     1621 acatggtgag gatggccggc agcgggtaca gccacatctt gaaagggaag cgttcgggag
     1681 gccaacggcg gtgcaacagt attaagccaa tggcctgtcc gataaattgc acgagcaggc
     1741 gggtggcgag tatcgcggcg attacagtgg acagcctgaa aagcaggctg gatacaaaag
     1801 ctaccgcgcc gaggaataat aacgacacgt gaggaaattt cttttgcgga tgcacgcgcg
     1861 caaaaatgga aaaaaagctg ccgtccagcg ccgccgcgta aggaatacgc gaatatccca
     1921 gcagggacgc gaaaatggac gcaaaggcga tccacaatac gagcatggtg gcgatgcgag
     1981 cagcgaacgg gcctgaaata gtctctacga aagtgctcac gaggaatggg gaattctgag
     2041 c
//
LOCUS       DATCBB010000556         1705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_408179, whole genome shotgun sequence.
ACCESSION   DATCBB010000556 DATCBB010000000
VERSION     DATCBB010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408179"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VII37_06100"
     CDS             <1..602
                     /locus_tag="VII37_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5069336.1"
                     /translation="RGSTLLMIIVALVGLIVAVAVSLLHNGLSARARPSAMEEMLARN
                     AWHLSIPSEARAMSNPLAASAENLKDARLHFADHCAICHANDGSGDAPFGRGLYPKPP
                     DLRQPETQKRSDGELFWIIENGVRFTGMPSFASEGMSDDSWKLVLFIRHLPQLTAEER
                     MEMSRYNPKSPEEREEEKQEDEFLNGTTPQKSGTPNSHP"
     gene            662..1003
                     /locus_tag="VII37_06105"
     CDS             662..1003
                     /locus_tag="VII37_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069337.1"
                     /translation="MFAILLSFLFVAITLSAHGDKKHVIGTIEKLNSGSVTVKTRDGK
                     SVQVKLLPSTVYVSRVATADKPAALSDLAVGENVVIHATPKGEDLEADEVRFSISGAS
                     PSASSKTQQHP"
     gene            complement(1034..>1705)
                     /locus_tag="VII37_06110"
     CDS             complement(1034..>1705)
                     /locus_tag="VII37_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY5069338.1"
                     /translation="ALARSLATFPRLLLLDEPLTALDEGLKKSIMEDLRRWNAAQNIP
                     ILYVTHSRAEVDALGERVIVMDQGKIISTGTPHEVLDTPRRSSLAQATGFENLFSGVV
                     TEIREADGVMRVRLVSGACEIETPLGDAVAGTAVKLAIRAGDILLATERPYGLSARNV
                     IPGRVASLEQRGTMFIAQVATNSDGAVVFTVHLTLGAKRALELDLNRPVWLVLKTHSC
                     HVLQD"
BASE COUNT          374 a          459 c          468 g          404 t
ORIGIN      
        1 gacgcgggtc cactctatta atgattatcg tcgcgttagt tggcctcatc gttgcggtcg
       61 ccgtttcgct tctgcataac ggcttgagcg ctcgcgcgcg gcccagcgct atggaagaga
      121 tgctggcgcg taacgcgtgg cacctttcca ttccatcaga ggcgcgcgcc atgagcaatc
      181 cactggctgc ctccgcggaa aatcttaaag atgcgcgtct ccatttcgcg gatcactgcg
      241 cgatttgtca cgcaaacgat ggcagcggtg acgctccatt tggacgcggc ttatacccaa
      301 agccgccgga tttgcgccag cctgaaacgc agaagcgctc ggatggtgaa cttttctgga
      361 tcatcgagaa cggagtgcgg ttcacgggca tgccgtcatt tgctagtgaa ggtatgagcg
      421 atgattcgtg gaaattggta cttttcataa ggcatttacc gcagcttacc gccgaagagc
      481 gcatggaaat gtcccgctac aatccgaaaa gcccggaaga acgcgaagaa gagaaacagg
      541 aagatgaatt tctgaatggc acaacgccgc agaaatccgg aacaccaaat tctcatcctt
      601 agggtattct tgaaaaggca ttcttgattc tgtaagtctt gggggcttta tgacgcgtcg
      661 cgtgttcgca atcttgttat cttttctatt tgttgctatc accctttctg cccacggcga
      721 caaaaagcac gtcatcggca cgattgagaa attaaattcc ggctcggtga cggtgaaaac
      781 ccgcgatgga aaatcggtgc aggtgaaact tttgccttct actgtatatg tttcacgggt
      841 ggctacggcg gataagcctg ccgcgctttc cgatctcgcc gtcggtgaaa acgtggtgat
      901 tcatgccact cctaaaggcg aggacctgga agccgacgaa gtcaggttct ccatctcagg
      961 cgcgtctccc tctgcttcgt cgaaaactca gcagcaccct tagggtttac tcgacgcagt
     1021 atggatttta tttttagtct tggaggacgt ggcaggagtg agtttttaac accagccaca
     1081 cagggcggtt taaatccagt tctaaggcac gtttggctcc gagggtcagg tgcacggtga
     1141 aaacgacggc accgtcggag ttggtcgcca cctgtgcgat gaacatggtg ccgcgttgtt
     1201 cgagcgaggc gaccctgcct ggaatcacgt tacgggcgct caatccgtaa gggcgctcgg
     1261 tggccaatag aatatctccg gcacgaatcg ccaatttgac ggcggttcct gcgacggcgt
     1321 cacccagcgg cgtttcgatt tcacacgcgc cgctcaccag gcgcacacgc atcacgccat
     1381 ccgcttctcg aatttcggtc accacaccgc tgaacagatt ttcaaatcct gtggcttgtg
     1441 ccagggatga acgccgcggc gtgtccagca cctcgtgagg cgttccagtg ctgataattt
     1501 ttccctgatc cattacgatc acgcgttcgc ctagcgcatc gacctcggcg cggctgtggg
     1561 tgacatacaa aattggaata ttttgcgccg cattccagcg ccgcaaatct tccatgatgg
     1621 atttttttag gccttcgtcg agtgccgtca ggggctcgtc gagaagcagc aatcgcggaa
     1681 aagttgccag tgaacgagcc agtgc
//
LOCUS       DATCBB010000557         1308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_439679, whole genome shotgun sequence.
ACCESSION   DATCBB010000557 DATCBB010000000
VERSION     DATCBB010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439679"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VII37_06115"
     CDS             <1..285
                     /locus_tag="VII37_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5069339.1"
                     /translation="PGFGDQREVELLVEAGFTPVQAIHVATENGARYLGQEDRIGTLA
                     AGKQADIVLIKGDPSKNIADIENVETVFKAGVGYDSAKLTELVRGQVGIR"
     gene            321..>1308
                     /locus_tag="VII37_06120"
     CDS             321..>1308
                     /locus_tag="VII37_06120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5069340.1"
                     /translation="MIAATRRVLSFIFICAAVTSIGAAQAQSPGATPNSPAAAAPAAR
                     GHAAAQPFDVNAAVEAYLAKMSPEQRARSNAYFEGGYWLQLWDFLSTVLVMWLLLRFG
                     WSKRMRDLAERITRFRPLQTALYWVQFIIAIYVVTFPLTVYEAYFREHKYGLLNQTFG
                     PWMRDQLVMLAVNIVLGGILVVVLFGVVRRLGKTWWVWGAVVSVVFLAFASLIAPVYI
                     FPLFNKYTKLQDARIKDPIISLARGNGIPASDVYEFDASRQSNRVSANVSGFAGTLRI
                     SLNDNLLRRCTLAEIETTMGHEMGHYVLNHSYQGLVMNGVVIVIAFAFVSWGI"
BASE COUNT          267 a          326 c          416 g          299 t
ORIGIN      
        1 ccgggatttg gcgatcaacg cgaagtggag ctgctggtgg aagcaggatt cactccggtg
       61 caggcgattc acgttgccac ggaaaatggc gcacggtatc tgggacaaga agaccgcatc
      121 ggcacactcg ctgcgggtaa acaagcagac atcgtgctga tcaaaggcga cccttcgaag
      181 aatattgctg acattgaaaa cgtcgaaact gtctttaagg cgggagtagg atacgactcg
      241 gcgaaattaa ccgaactggt gcgcggtcag gtggggattc gttagtaaac cggagattcg
      301 gctggccaga gggggagaga atgatcgctg caacgcgacg tgtactttct tttattttta
      361 tatgcgctgc tgtgacgagc attggcgcgg cacaggcgca gagtccaggc gcgactccga
      421 attctccggc ggccgccgct ccggccgcgc gtgggcacgc ggcggcacaa ccatttgacg
      481 tcaacgccgc cgtggaggca tatctggcga aaatgtcccc tgaacaacgg gcgcgttcga
      541 acgcatattt tgagggcggt tactggctgc agctgtggga ttttctgtcg acggtattgg
      601 tcatgtggct actgttgcgc ttcggctggt cgaagcggat gcgcgatctg gccgagcgca
      661 ttacgcgctt tcgtccgctg cagacggcgc tttattgggt gcaattcatt atcgcgatat
      721 acgtggtgac ctttccgctg actgtgtatg aagcgtactt ccgtgagcac aaatacggcc
      781 tgttgaacca gacctttggc ccgtggatgc gcgaccagct ggtcatgctg gcggtgaaca
      841 tcgtcctggg aggaattctg gtggtggtgc tcttcggggt ggtgcgacgg ctgggaaaaa
      901 cctggtgggt gtggggagcg gtggtaagcg tcgtgtttct ggcgttcgcc agtttgattg
      961 ccccggttta catttttccg ctgttcaaca aatacacgaa gttgcaggac gcgcgcatca
     1021 aggatccgat catcagcctg gcgcgcggta acggaattcc tgcatccgat gtgtacgagt
     1081 tcgacgcttc gcgtcaatcg aatcgcgtca gcgccaacgt cagcggattc gcgggcacgc
     1141 tgcgcatttc gctgaacgac aatctattga ggcgctgtac gctggcggaa attgaaacca
     1201 ccatgggcca cgagatggga cattacgttc tgaatcactc ctaccaagga ctggtcatga
     1261 atggcgtggt gatcgtgatc gctttcgcat ttgtcagctg gggcatcg
//
LOCUS       DATCBB010000558         1098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_408316, whole genome shotgun sequence.
ACCESSION   DATCBB010000558 DATCBB010000000
VERSION     DATCBB010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408316"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..>1098
                     /locus_tag="VII37_06125"
     CDS             253..>1098
                     /locus_tag="VII37_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY5069341.1"
                     /translation="MRRTVHISGQIEPFFGTLDDNVRLLEDVLHVRTHLHDTRLTIEG
                     DQDNVDQAVKIVEEYNHLTRHGRTLTSAEVKSLMRVATEVPHESPRTMFEPTRTRSFG
                     KKQVTPKNASQRRYMEEIEKHDMMFGIGPGGTGKTYIAVAMAVSALLTKQVNRIILAR
                     PAVEAGERLGFLPGTLQQKIDPYMRPLYDALYDMLDADKLERFLEKGIIEVAPLAFMR
                     GRTLNDSFVILDEAQNTTSEQMKMFLTRLGFNSKAVITGDVTQIDLPTGKKSGLVEAL
                     EVCGKI"
BASE COUNT          254 a          290 c          330 g          224 t
ORIGIN      
        1 aaagttgggt gcggctttgg acactttcgt tccggtacgc tgtcttcgct gcggaggtgc
       61 tattccgtct gggtggcgcg ccgatggcgc gattgcacaa caagctgcct gaaccttgac
      121 cgcgtcgaat ggctgggata gggtggaaga agtttcttcc cgagtcgatt gccgcattta
      181 gcctagcccg gaggagccgt cggccactca ttgcccgtaa ctgggcctgt agctggaaaa
      241 cctgccaact ggatgagacg taccgtacac atatccggac aaattgaacc gttttttgga
      301 acgctggatg ataacgtgcg tttgcttgag gatgtgctgc atgtgcgcac gcacctgcac
      361 gacacccgct tgaccattga aggcgatcag gacaacgtcg atcaggcggt gaagatcgtc
      421 gaagagtaca accacctgac tcgccacgga cgcaccttaa ccagcgcgga agtaaagtcg
      481 ctcatgcgcg tggccaccga ggtgccgcac gaatccccac gcaccatgtt tgaaccgacg
      541 cgcacgcggt cgtttggcaa gaaacaggtc acgccaaaaa acgccagcca gcgacgctac
      601 atggaagaaa ttgaaaagca cgacatgatg tttggcatcg ggcctggcgg caccggcaaa
      661 acttacatcg ctgtggccat ggcggtcagt gcgctgctga ccaagcaagt caaccgcatc
      721 atcctcgcgc ggcctgcggt ggaggccggc gaacggctgg gatttttgcc gggaacactg
      781 cagcagaaga tcgaccctta tatgcggcca ttgtacgacg cgttgtatga catgttggat
      841 gccgacaagc tggaacgatt tctggagaag ggcatcattg aagtggcgcc actggcgttt
      901 atgcgtgggc gcacgctgaa tgattcgttt gtgattctcg acgaagcgca aaacaccact
      961 agcgagcaga tgaaaatgtt tttgacgcgc ctgggtttta attccaaggc ggtgattacc
     1021 ggcgatgtca cgcaaatcga cctgccgact gggaaaaaat caggcctggt ggaggcgctg
     1081 gaagtctgcg gcaagatc
//
LOCUS       DATCBB010000559         4986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_374966, whole genome shotgun sequence.
ACCESSION   DATCBB010000559 DATCBB010000000
VERSION     DATCBB010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374966"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1450
                     /locus_tag="VII37_06130"
     CDS             68..1450
                     /locus_tag="VII37_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5069342.1"
                     /translation="MLALKYLLMLLGVGLFGSAGALVVYDVYVSERLRRLIKRNASTD
                     KDAPVHLPALPLAPVRWKQAQQLAVAAVLPVLLALSIVVIPDGSAGVRISQIWGARPG
                     TFYPGVHIVTPLIDSVALYDTREQVYSTVAAETDKKKGEVLSVQAREGLNIGLAVSVR
                     YRLDPRRLDSIHTNLPQPVGEQVIAPVVSTIYRQLAPNYMTREIFATKREELRSKAAE
                     ASTARLASDGIVVREVLLRDLQLPEEYAKGLEGLLLKEQEDERLTTEQDIKFKEVKIA
                     ELEAEAQKARSVKQAEAQAQVRVLQAKSESDAMQYTLPLKQKQIEQTKLEAQARKEAT
                     LQNAEAAAQAKIIDSKAEVERQRNLSEAEANRIRVTAAADSERMKFEAAVLKSNPMLI
                     QKIIAERLSDKLQIMMVPMDGRNFFASDVMRSAFSGLGSTNGNAADDADPSSQPTTRA
                     AVQGKAARRP"
     gene            1620..3491
                     /locus_tag="VII37_06135"
     CDS             1620..3491
                     /locus_tag="VII37_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069343.1"
                     /translation="MSLRTKLLLIFMLTVVTSVSVVAYGVTHYTRAAFEEMDAQRTEA
                     LVAQFQKEFAQRGEEVVHQVENIANAEITLRMALDLARPNADQSLYVHDGTGAAQDHG
                     LDFVQFVNSDGTLISSAQYPSRVGYKNEWVTANKNWRDSHAFLNKEELPDGVALSLTV
                     VRTLPVGDKNFYIIGGRRLDQNFLASLVLPAGMRTLLYQNLEPGFVPAELTDAGGAAK
                     EPERYASLIEQSKQQPQRPVIGTLNWSADPASAETFHAIPLTGRNDELLGILLVGSSR
                     QELVLLTERIGWIAASVGAGALLIALLLIWWFSARITHPVEELAAASREVASGRWDAR
                     IDVRSYDEVGQLATAFNDMTRTLAVHKERLLQTERVAAWRELARRLAHELRNPLFPLQ
                     ITVENLQRARQLDHEQFMEVFTESTATLKTELANLNSIVARFSDFSKMPTPRFERMDV
                     NEALREVLRLFDAQFNQVGKPAITRELFLKEGLPEIDGDSELLHRAFQNVVLNAVDAM
                     PEGGTLTLRTSDLEGKIRVEIADTGNGLTSEESARLFTPYFTTKPQGTGLGLAIVQSV
                     VSDHHGTVSISSEEGRGTTIRIELPQRQSERLETPHIEVVTAKSEPTTPSTLAAASD"
     gene            3509..4903
                     /locus_tag="VII37_06140"
     CDS             3509..4903
                     /locus_tag="VII37_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5069344.1"
                     /translation="MPRKAHLLLVDDDPNTLASLSRAFRMAGHEVTVCDNAARALETL
                     RSESFDLVFSDVVMPGKTGLDLLEEMKSLGIKTPIIMISGQANIEMAVRATKLGALDF
                     LEKPLSTDKLLLTIENALRLTRLESENQELRRRIGKHELVGGGAAMKKLLSEIERVAA
                     SETRVCILGETGTGKELVARAIHEKSARKENAFVTLNCAAVPAELIESALFGHEKGSF
                     TGAAAKHLGKFEQAEGGTLFLDEIGDMPLAMQAKLLRVLEEAEVERVGGDKPIRVNVR
                     VLVATHRNLENLVKENGFRQDLFHRIYVFPLRVPPLRERKEDMPELIAHFASQVASQN
                     GWKQKSFDEEAVEELRHYTWPGNVRELRNVVERLILLSDGNTVNGDDVRLVLPGYAGN
                     TADSVAGTNHHETTGTLAERSEAFERETLISEIRRQNFHMTNVARALGLERSHLYKKC
                     QQLGIDLQAIRKPA"
BASE COUNT         1202 a         1279 c         1466 g         1039 t
ORIGIN      
        1 cgccgctccg cgacagcact gtgaagaatt tttatgtgca ggaccacaga aagaagggag
       61 caataccatg ttggctttaa aatatctgct gatgcttcta ggtgtcggcc tgtttggcag
      121 cgccggggcc ttggttgttt acgatgtata cgtttctgaa cgattgaggc gattgatcaa
      181 gcggaacgca agtactgaca aggatgcgcc ggttcattta ccagcgctcc ctttagcccc
      241 ggtgcgttgg aagcaggcgc aacaactagc cgtcgcggcc gttttgccag tgctgctggc
      301 gctcagcatc gtggtcattc ccgacgggtc ggcgggagta cgcatcagcc agatttgggg
      361 cgcacgtccc ggcacgttct atccgggagt gcacatcgta acgccactga tagatagcgt
      421 ggcactgtat gacactcggg aacaggtata ttccacggta gcagcggaga ccgacaagaa
      481 aaaaggcgaa gtgctctccg ttcaggcgcg tgagggtttg aatattgggc tggccgtctc
      541 ggtgcgctac cgtctcgatc cgcggcgtct cgattcgatt cacacgaacc tgccgcaacc
      601 ggtcggcgag caagtgatcg cgccggtggt ctcgacgatc taccggcaat tggctcctaa
      661 ttacatgacc cgcgaaatat tcgcgaccaa gcgggaagaa ttgcgttcga aagccgcgga
      721 agcgagcact gctcggttgg caagcgacgg catcgtcgta cgcgaagtct tgctgcgcga
      781 tctgcaattg cccgaggagt atgccaaggg tctcgaaggg ttgttgctga aagagcagga
      841 agacgaacgc ctcactaccg agcaggacat taaattcaag gaagtgaaga ttgccgagct
      901 ggaagcggaa gcgcaaaaag ctcgctccgt gaagcaggcc gaagcgcaag cgcaagtgcg
      961 agtgctacag gccaaatccg aatcggacgc gatgcagtac accctgccgc tcaaacaaaa
     1021 gcagattgaa caaaccaagc tggaggccca agcacgcaaa gaagctacac tgcaaaatgc
     1081 cgaagccgcg gcgcaagcta aaataatcga cagcaaagcc gaagtggagc gccaaaggaa
     1141 tttgtcggaa gcggaggcga atcgcatccg ggtaactgcg gcggctgatt cggagcgcat
     1201 gaaattcgaa gctgcggtgc tgaaatccaa tccgatgctc attcaaaaaa tcatcgccga
     1261 acgcctttca gacaagctgc aaatcatgat ggtgcccatg gacggcagga atttctttgc
     1321 cagcgacgtg atgcgatcgg cattctcagg tttgggtagc actaacggca atgctgccga
     1381 cgatgcagat ccgtccagcc aaccgacgac gcgggcggcg gtgcagggta aagcggcacg
     1441 gcgtccttga tactaggtag cgataccagg gcacaaaggc agcgtggatg gactaattga
     1501 cggcccacct cgctgctggc tcttttacca cgacgcttaa tcgggatatg ctttcttctt
     1561 cgcggaagga caaagctaat tagcacgatc ggtctgaaaa gagtttctgg agtatttcgg
     1621 tgagtctccg caccaagttg ctgctgattt ttatgctgac cgtggtgacc tcggtctccg
     1681 tagtggctta cggcgtgacg cactacacgc gcgcggcgtt tgaggaaatg gatgcgcagc
     1741 gcaccgaggc cctggtggcg caattccaga aggaatttgc acagcgtggc gaagaggtcg
     1801 tgcatcaggt tgaaaatatc gccaatgcgg agatcacctt gcgcatggct ttggatctgg
     1861 cgcgccccaa cgccgaccag tctttgtatg tgcacgacgg tactggggcg gcccaggacc
     1921 acggcctgga tttcgtgcag ttcgtcaatt cagatggcac gctgatctcc tcggcgcaat
     1981 atccttcgcg ggttggttac aaaaacgagt gggtcaccgc caataaaaat tggcgagact
     2041 cgcatgcatt cctgaacaaa gaagagttgc cggacggcgt cgcactttcg ttgacggtcg
     2101 tgcgaacctt gccggtgggt gataagaatt tttacatcat cggcggtcgc cggcttgatc
     2161 aaaattttct ggcttcgttg gtgctgcccg ccgggatgcg tacgctcttg tatcagaatc
     2221 tggagcccgg ctttgttcca gcggaattga cggacgcagg cggcgcagct aaagagccag
     2281 agcgatatgc ttcactgatc gaacaaagta aacagcagcc acagcgaccg gtgattggca
     2341 ctctcaattg gagtgcggat ccagcgagcg ccgaaacatt tcacgccatt ccgctcacag
     2401 gccgaaacga tgagttactg ggaatactgt tggtgggcag ctcgcggcaa gaattggtgt
     2461 tgttaacgga acgcattgga tggatcgccg catccgtggg agctggcgcg ctgctgattg
     2521 ccttgctgct gatatggtgg ttttccgcgc gaattacgca cccggtagaa gaattggccg
     2581 ccgcgtcgcg cgaagtggca tccggaagat gggatgcgcg cattgacgtg cgcagctacg
     2641 acgaagtagg acaattggcg actgcgttta acgacatgac gcgcacgctg gcagtccata
     2701 aggagcggtt gctgcaaacc gaacgggttg ccgcctggcg ggaattggcc cgaagattgg
     2761 cgcatgaact gcgaaacccg ctgtttcccc tgcaaataac cgtcgaaaat ctgcaacgtg
     2821 cgcggcagtt ggaccatgaa cagttcatgg aggtttttac cgaatcgaca gcaacattga
     2881 aaacggaatt ggctaacttg aattcgattg tggcgcgctt cagtgatttc tcaaaaatgc
     2941 caacgcccag attcgaacgg atggatgtga acgaagcgct ccgcgaagtg ctgagacttt
     3001 ttgatgcgca gtttaatcaa gttgggaagc cggccatcac gcgcgaattg ttcctaaagg
     3061 aaggcttgcc agagattgat ggagattccg aattactgca ccgcgcgttt cagaatgtcg
     3121 tgttgaacgc tgtagatgcc atgcctgaag gcggaacgct cacactgcga acctcagatc
     3181 ttgaaggaaa gattcgcgtg gaaattgccg acaccggcaa tggcttgacg tctgaagaat
     3241 cagcccgact gttcaccccg tacttcacga caaaaccaca aggaacaggt ctcgggctgg
     3301 cgattgtgca atccgttgta agcgatcacc atggcacggt ttcgatctcc agtgaagaag
     3361 gccgcggcac gacgattcga attgaattgc cgcagcgcca gtcggaacgc ctagaaactc
     3421 cgcatattga ggtcgtaact gcaaaatccg agccgaccac accgagcaca ttggccgccg
     3481 cctcggacta aggcttcaag gagctcgctt gccgcgcaag gcccacttgc ttcttgtcga
     3541 cgacgatcct aatacgctgg cgtcgctttc gcgcgctttt cgcatggccg gccacgaagt
     3601 aaccgtttgt gacaacgctg cccgcgcgct agagactctc cgtagtgaat catttgacct
     3661 ggttttttcc gatgtggtga tgcctggcaa aacgggactg gatctgctgg aagaaatgaa
     3721 aagcctgggc atcaagactc ccatcatcat gatttccggg caagccaaca tcgaaatggc
     3781 ggtacgtgct accaagcttg gcgcgctgga ctttctggag aaaccactct ccaccgacaa
     3841 attgttgctg accattgaga atgcactgcg cctcacgcgg ctcgagagtg aaaatcagga
     3901 gctgcggcgt cgcatcggga agcatgagct cgtgggcggc ggtgccgcga tgaaaaaact
     3961 cctgagcgaa atcgagcgtg tagccgctag cgagacccgc gtgtgcatat tgggcgaaac
     4021 gggaaccggt aaggaactgg tggcgcgagc cattcacgaa aaatctgctc ggaaagagaa
     4081 tgcattcgtg accttgaatt gtgcggcagt ccctgccgaa ctgatcgagt cagcattatt
     4141 cggccatgaa aagggctcgt ttacaggagc ggccgccaag catcttggaa aattcgaaca
     4201 agccgaaggt ggcacgttgt ttctggacga aattggcgat atgccgctgg cgatgcaggc
     4261 aaaacttttg cgcgtgcttg aagaagcaga agtggaacgc gtgggcgggg acaagcccat
     4321 ccgcgtaaat gtccgggtac tggtggctac gcatagaaat cttgagaatc tcgtcaaaga
     4381 aaatggattt cgtcaggatt tattccaccg catttacgtt ttcccgctgc gtgtgccgcc
     4441 attgcgcgaa cgaaaagaag acatgcccga attgattgcg cacttcgcga gtcaggttgc
     4501 ttcgcagaac ggctggaaac aaaaatcctt tgatgaggaa gcggtggaag agctgcgaca
     4561 ttatacctgg ccgggaaacg tgcgggagtt gcgcaatgtt gtggagcgtt taatcctgct
     4621 ttccgatggc aatactgtaa atggtgatga cgtgaggctg gtgcttcccg gttacgccgg
     4681 caacaccgca gatagcgttg ctggtacaaa tcatcatgaa accaccggca ctctcgcaga
     4741 gcgcagcgag gcgttcgagc gtgagacgct aatctctgag attcgacgcc agaattttca
     4801 catgaccaac gtggcgcgcg ctttgggatt ggagcgcagc catttgtaca agaagtgcca
     4861 gcaactgggc atcgacctac aggccattcg caagcccgca tgaggggtcc aagcctttgc
     4921 aggtccggca aaaatcccta cctgacgttg cctttgtgca tgtcaaaatg aagtcgtgaa
     4981 tccatg
//
LOCUS       DATCBB010000560         1341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_148758, whole genome shotgun sequence.
ACCESSION   DATCBB010000560 DATCBB010000000
VERSION     DATCBB010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148758"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VII37_06145"
     CDS             <1..305
                     /locus_tag="VII37_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069345.1"
                     /translation="NWVLDAGMKAEPSLAAVLYEPTSGRVVEAWTTEPGIQVYTGNFL
                     DGTSHGKGARNYPRRSAVCLETQHFPDSPNHPDFPTATLVPGREFHSITIYKFSAK"
     gene            441..>1341
                     /locus_tag="VII37_06150"
     CDS             441..>1341
                     /locus_tag="VII37_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY5069346.1"
                     /translation="MERIYSEPGLSGHMLRGMAWTPDGKQVTFLEAESSDADADGAAE
                     DGVKNTRKDLQKKENKAALWIADAKSGDRRILVPAERLASMLPENSAAPTQATGLGRH
                     APADYQWAPNGEALLFQGPNALVWFELKSEAPHTLVAGKETLADPKISPDGKFVSFVR
                     DHNLWLVNIVDGQERAITRDGVEELRKGELDWVYPEELETKTAYWWAPDSSAIAYLEM
                     DERKVAQYPLVDFSSFDGKAEMERYPPAGGANPVVHVFVAPVSGGPPKQMDTGSETDQ
                     YIPRVNWLPDSKHVSIQRLNRPQT"
BASE COUNT          320 a          359 c          387 g          275 t
ORIGIN      
        1 acaactgggt actggatgcg ggcatgaaag cagaacccag tttggccgcg gtactttatg
       61 aacccacgag tggccgcgtc gtggaagcgt ggacgaccga gccgggtatc caggtctaca
      121 ccggcaattt tttggacggc acctctcacg gcaagggcgc cagaaactac ccgcgccgtt
      181 cggcagtgtg tctggaaacg cagcactttc cggattcgcc gaatcaccct gattttccaa
      241 ccgcgacgct cgtgccgggc agagaatttc actccatcac aatctacaaa ttttcagcga
      301 aataggagcg tatgccctca cgaagcatca tcactcgtca tccgattatt ttttgcgagt
      361 tcattacatt gtgtatggtt ttttcgggcg cgacgtgcgc ccagactgat gatgcctcca
      421 agtccggaaa gaaactcacc gtggaacgta tctattccga accgggtctg agtggtcaca
      481 tgctgcgagg gatggcgtgg actcccgatg gcaaacaggt gaccttcctc gaagcagaaa
      541 gctccgacgc cgacgccgat ggcgctgccg aggacggcgt taaaaatacc cgtaaggacc
      601 tgcagaaaaa agaaaacaaa gccgcactct ggatcgcgga tgcgaagtcc ggtgaccgtc
      661 gaattctcgt acccgcagag cgccttgcgt ccatgctgcc agaaaattcg gcggctccca
      721 cgcaggctac ggggctcggt cgccacgcgc ccgccgatta ccaatgggct ccaaatggag
      781 aggcgctttt atttcagggg ccaaacgcgc tggtatggtt cgagttgaag tctgaggcgc
      841 cgcacacttt ggttgccggt aaagaaactt tagcagatcc aaagatttcg ccggacggca
      901 aatttgtcag ctttgtgcga gaccacaatc tctggctggt aaatattgtg gacggccaag
      961 agcgcgcgat tacccgcgat ggagtggaag aactccgcaa aggtgagctg gactgggtgt
     1021 acccagaaga gttggaaact aaaaccgctt actggtgggc gccggattcc tccgcaatag
     1081 cgtatctgga aatggatgag cgcaaagtgg cgcaatatcc gctggtggat ttttcgtcat
     1141 tcgacggtaa agcggagatg gagcgttacc ctccggctgg cggcgcgaat cccgtggttc
     1201 atgtcttcgt agcgcctgtg agtggaggac cgcccaagca aatggacacg ggctccgaaa
     1261 cggatcaata cattccgcgg gtgaattggc tgccggattc aaagcacgtg tctatacaac
     1321 ggttgaaccg gccacaaaca a
//
LOCUS       DATCBB010000561         1641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_18796, whole genome shotgun sequence.
ACCESSION   DATCBB010000561 DATCBB010000000
VERSION     DATCBB010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18796"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..835
                     /locus_tag="VII37_06155"
     CDS             59..835
                     /locus_tag="VII37_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069347.1"
                     /translation="MHPAIPHLIELQRVDHNIGALRAEIDSYPKRVQAADARLSGARA
                     AVSAAKEAQTNALKERKKFELDVDQWKDRAKKYRDQSGAVKTNEAYKALLHEIANAEA
                     EVAKAEDRQLDVMMAGEEFERRVRHAEADLKEAETSVAKDRKEIQQLHTEKQKQMAAA
                     LAERARIIVPVPEDLLELYTRIAKRHNGTAMAQVKDDQCRGCGMRALPHIVQELRQEP
                     NEEIYRCEMCGLILYSLEPIPAAKTGTDEAKSAATPSANS"
     gene            892..1479
                     /locus_tag="VII37_06160"
     CDS             892..1479
                     /locus_tag="VII37_06160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEY5069348.1"
                     /translation="METKPLPAAYQANIDGGSRGNPGPASYGVVVRNERGEIVAKLKK
                     YIGRMTNNVAEYYGLIAALDYAQSHGVRALRIESDSELLVRQMRGHYKVKSADLKPLF
                     ERAKKMSQTFESFRIDHVYRAQNSEADTLANEALDETAGRTKAPTAIQKNDSLAEVPR
                     KLRAHYRDGGLQLLERLDLPENTEVEILLRLPPKR"
BASE COUNT          409 a          455 c          511 g          266 t
ORIGIN      
        1 tcgtggcgct tcgccgcgca gccgggcgga caagtgcagc cgtgttagaa tcgtctgaat
       61 gcacccggca atacctcacc tgatcgaact gcagcgggtt gaccacaaca tcggcgcgtt
      121 gcgcgcggaa atcgacagct atcccaagcg agttcaggcc gcggacgcca gactgagcgg
      181 cgcgcgcgcg gcggtgtcgg ccgcgaaaga agcgcagacc aacgctctga aagagcgcaa
      241 gaaattcgag ctggacgtcg atcagtggaa agaccgcgcg aaaaaatatc gcgatcagag
      301 cggcgcggta aaaaccaacg aagcgtataa agcgctgctg catgaaattg ccaacgccga
      361 agcagaagta gccaaagccg aggaccgcca gctggatgtg atgatggcgg gagaagaatt
      421 cgaacgccgg gtgcgccacg ccgaagcgga tttgaaagag gcggaaacca gcgtggcgaa
      481 ggaccgcaag gaaatccagc agctgcacac tgaaaaacaa aaacaaatgg ccgcggcgct
      541 ggccgaacgc gcgcgtatca ttgttcccgt gcctgaagat ctgctggaac tgtacacccg
      601 catcgccaag cggcacaacg gcacggccat ggcgcaggtg aaggacgatc aatgccgcgg
      661 gtgcggcatg cgcgcactgc cgcacatcgt tcaggagctt cgccaggaac ccaacgaaga
      721 aatctatcgc tgcgaaatgt gcgggctgat tttgtattcg ctggagccga ttcccgcggc
      781 aaagacgggc accgacgagg cgaagagcgc ggcgacgcct tctgcaaact cctgatggcc
      841 acgcgccgtt caccagctcc gcgcgcaaac ggtttattca cagacgcgga gatggaaacg
      901 aaacccctcc cagccgctta ccaggccaat attgatggag gttcacgggg gaatcccggg
      961 ccggcttctt atggcgtggt ggtacgcaac gaacgcggcg aaatcgtcgc gaaattaaaa
     1021 aaatatatcg ggcgcatgac caacaatgtg gcggaatact acggcctgat cgccgcgcta
     1081 gattacgcgc agtcccatgg agtgcgcgcg ctgcggattg aaagcgactc cgagctgctg
     1141 gtgcggcaga tgcgcgggca ttacaaagtg aagagcgctg acctgaagcc gctattcgag
     1201 cgggcgaaga aaatgtcgca aacgttcgaa tcgtttcgca tcgatcacgt ttaccgggcg
     1261 cagaattccg aagcggatac gctggccaac gaagcgctgg atgaaacggc cgggcgaacc
     1321 aaggcgccga cggcgataca gaagaacgac tcgttggctg aagtgccgcg caagctgcgg
     1381 gcgcattacc gtgatggcgg attacagctg ctggagcggc tggacttgcc ggaaaatacc
     1441 gaagtggaaa ttctgctgcg tctcccgcca aagcgttagc aatctcatta tttcagcgag
     1501 taagaacggt agagcgtatc gcgttgcgca ggcacgaagc cggcgtcgga gataatgcgg
     1561 cgcagctcgt tttcgttggt gcgatttctt actcccgcgg cgaaaaccac attttcttca
     1621 atcaggatgc tgcccacgtc g
//
LOCUS       DATCBB010000562         1037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_310440, whole genome shotgun sequence.
ACCESSION   DATCBB010000562 DATCBB010000000
VERSION     DATCBB010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310440"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..767
                     /locus_tag="VII37_06165"
     CDS             48..767
                     /locus_tag="VII37_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY5069349.1"
                     /translation="MTLRERLEWVSRMSLLVFILASAAFLSAITAMRVAIHGREVSMP
                     NLVGKNVSEASQLLRSRGLVLRVADRVYSDLPTNVVVRQTPTAGLLMKVSQQAHVVLS
                     LGQRQLSIPRLEGTTLRISRIEMLREGLQIGEVSNITLPNVPADTVVIQNPKPGTGAA
                     TPRVDVLVSQGDRDKAYVMPHLVGLNETDAQHRLDVAGLRRKVNYVTAPQWPHGAVVD
                     QTPLAGSRIPASAPVELTIAL"
BASE COUNT          226 a          250 c          329 g          232 t
ORIGIN      
        1 aagacaaagg ttcggactga tcttttttta aagctgagat tccatttatg acgctgcgcg
       61 aacgactgga atgggtaagc aggatgtcgc tgctggtttt tattctggcg tcggcggctt
      121 ttctcagcgc aattacggcc atgcgcgtgg ccattcacgg gcgcgaagtg agcatgccga
      181 atcttgttgg gaagaacgtc agcgaggcta gccagctttt gaggtcgcgg ggtttggttt
      241 tgcgggtggc agaccgggtg tatagcgatt taccgacgaa cgtggtggtg cggcagacgc
      301 cgactgcggg attgttgatg aaagtttcgc agcaagcgca cgtggtgctt agcctgggcc
      361 agcggcaact cagtattcct cggctggaag gaaccacgtt gagaatttcg cgtatagaga
      421 tgctgcgcga aggactgcaa atcggagaag tttccaatat tacgttgccg aacgtgcctg
      481 ccgatacggt ggtgattcaa aatcctaagc cgggaacggg ggcggctacg ccgcgggttg
      541 atgtgctggt atcgcaaggc gatcgcgaca aggcgtacgt gatgccacac ctggttgggc
      601 tgaatgaaac tgatgcgcaa caccggctgg atgttgcggg gttgcggcgc aaagtgaact
      661 acgtgacggc tccgcaatgg ccgcacgggg cggtggtgga tcagactccg ttagcgggtt
      721 cgcggattcc ggcttccgcg ccggtggagt tgacgatcgc gttgtagttt cgtcatcaca
      781 ggaaacgccg cgtaattcca atcccctttt tttgtgatga cacccgaaac caagcctggc
      841 tctcgataat tcaatcggga gcccagtagt tatgcgctgg gcgcttccac gttaaaaacc
      901 ccaccctcca aaaaaccgag ggtggggcac cctgctgaca tccaaaacaa ctccacacaa
      961 ttattgaagg gcgtatagat tgccgtcggt gctgccgaag tagagggtgt ctccggcgat
     1021 gacgggggaa cttagaa
//
LOCUS       DATCBB010000563         1022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_51485, whole genome shotgun sequence.
ACCESSION   DATCBB010000563 DATCBB010000000
VERSION     DATCBB010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51485"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VII37_06170"
     CDS             complement(<1..855)
                     /locus_tag="VII37_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEY5069350.1"
                     /translation="MRKLRISVVQYLNTAPLVRGFTHGPLKDKYDLSFTVPSQCADDL
                     RDGRADVAIIPAIEFQRIEDLVVLPDLAIAAKRRVRSLLIVSRGPIAEVQSIALDRSS
                     RSTQALTRILCAEHWHMKPAFFEETFNSTATILEHADAALVIGDPALRLAISIAGSAT
                     LGADGELMCSPSKAGVNATETLHVYDVVQEWRRLTGLPAVLAVWAGRRESITPDVVAD
                     FTASKDYGIAHLAEITATASVEQDLPAADLLNYLQDNIDFTLDSENLAGLELYYCLAA
                     KLGLIPSAK"
BASE COUNT          224 a          291 c          288 g          219 t
ORIGIN      
        1 cttggcgctc ggtatcaatc ccagcttggc cgccaggcaa taataaagtt caagcccggc
       61 gagattttcg ctgtccaacg tgaaatcaat attgtcctgc agataattca gcaaatcggc
      121 tgcaggcaaa tcctgttcca cgcttgcggt cgcggtgatc tccgcaagat gcgctatgcc
      181 atagtctttg gaagcggtga aatcggcaac cacgtccggt gtgatcgatt cacggcgccc
      241 ggcccacacc gccaaaaccg ccggcaggcc agtcaggcgg cgccattcct ggaccacatc
      301 gtacacatgc agcgtttctg ttgcattcac tccggctttg ctgggactgc acatcaactc
      361 gccatctgcc cccagcgtcg cgctgccggc gattgaaatc gcgagtcgca gcgctggatc
      421 gccgatcacc agcgcagcat cggcatgttc cagtatggtg gccgtggagt taaatgtttc
      481 ttcgaaaaaa gctggcttca tgtgccaatg ttcggcgcac aaaatccgcg tcaatgcctg
      541 cgtgctgcgg gaactgcgat ccagcgcgat gctttgcact tctgcgatag gcccgcgcga
      601 tacgatcagc agactgcgca cgcgtctttt tgcggcgatc gccaggtccg gcagcactac
      661 cagatcttcg atgcgctgaa actcgatggc cggaatgatg gccacgtcgg cgcgcccgtc
      721 gcgaagatca tcggcgcatt gcgagggtac cgtaaaggat agatcgtact tatccttcag
      781 aggtccgtga gtaaaaccgc gcaccaatgg ggcggtattc aggtattgca cgacggagat
      841 gcgaagtttt ctcaaaatgg cggacgctcg cggaagattc agtataacag cgaaaattta
      901 cgccgcgact gcggtcttct gttgtaaaac gttcgggacc tccggaggtt ttgaaaatga
      961 agaaatatac ccgcgccgtg cggctcatgc tggccttgca aatgaccgcg atcggtgggg
     1021 ca
//
LOCUS       DATCBB010000564         1044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_213196, whole genome shotgun sequence.
ACCESSION   DATCBB010000564 DATCBB010000000
VERSION     DATCBB010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213196"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..563
                     /locus_tag="VII37_06175"
     CDS             153..563
                     /locus_tag="VII37_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5069351.1"
                     /translation="MSRLEETLGQRTEELAATNRQLQRGVVRRKVMEDAAEANGKHHQ
                     KCLEESLQLQKRLRQLTHRVLATQEDDRTSISRELQDEIAQTLLGINVRLLSLKQEAR
                     RNTKGIKNEIASTQRLVLKSAKSVRRIAREFGSL"
     gene            560..>1044
                     /locus_tag="VII37_06180"
     CDS             560..>1044
                     /locus_tag="VII37_06180"
                     /inference="COORDINATES: protein motif:HMM:NF020257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase RsbU N-terminal domain-containing
                     protein"
                     /protein_id="HEY5069352.1"
                     /translation="MKRKFSRRYQAGLQAYLKQRSGAHLQRARGMGRQALNVGMQTLD
                     MAKLHEQILVSKVLPDSPAKKRAALIKQAGTFFTEAITPVEKNHHDTRVAAVHLNKIV
                     EMLSQRTVELAASNVELNAEIAQRKSVEAALKKSEHHYSQLLVESERLQQQLRQLSRQ
                     IL"
BASE COUNT          262 a          299 c          310 g          173 t
ORIGIN      
        1 tggatttggc ccggattcac gaacaggcac tcgctacctt ggaattatcg aacacaaaaa
       61 acgggttcac caagctggcc aggatttttt tcaccgaggc gaacattccc cttgccgaaa
      121 cgcatcgcgc cacgcgtaat acgaaaattc aattgagccg gctggaggaa accttaggcc
      181 agcgcacgga ggaactcgcc gccaccaacc gccagttgca acgcggggtc gtccggcgca
      241 aggtcatgga ggatgccgcc gaggcaaacg ggaagcacca ccagaaatgc ctggaggaat
      301 cgctccagtt gcagaaacgc ctgcggcaac tgacgcaccg ggttctggcg acgcaggagg
      361 atgaccgcac gagcatcagc cgcgaacttc aggatgaaat cgcgcaaact ttattgggca
      421 tcaatgtccg gctgctctcc ttgaaacagg aggcgcggcg caacacaaag ggtatcaaga
      481 atgaaatcgc cagcacgcag cggctggtgt taaagtcggc caaatccgtc cggcggattg
      541 cgcgcgaatt cggcagctta tgaaacgcaa attttcaaga cgttatcagg cggggttgca
      601 ggcttatctc aagcaacgct cgggggcgca cctgcagcgg gcgcgcggca tgggccggca
      661 ggcgctgaac gtcggcatgc agacgctgga catggccaag ctccatgagc aaatcctcgt
      721 cagcaaagtg ttgccggaca gtccggccaa aaaacgcgcc gcgctcatca agcaggcggg
      781 gactttcttt accgaggcga ttaccccggt tgagaaaaat catcacgaca cgcgcgtggc
      841 cgccgtccat ctgaataaaa tcgtcgagat gttgagccag cgcaccgtgg agctggccgc
      901 ctcgaacgtg gagttgaacg cggaaatcgc ccagcgcaag tcggtggaag ccgcgctcaa
      961 aaaaagcgag caccattaca gccagttgct ggtggagtcg gaacgcctgc agcaacagtt
     1021 gcggcaattg tcccgccaga ttct
//
LOCUS       DATCBB010000565         1992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_342911, whole genome shotgun sequence.
ACCESSION   DATCBB010000565 DATCBB010000000
VERSION     DATCBB010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342911"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..669
                     /locus_tag="VII37_06185"
     CDS             313..669
                     /locus_tag="VII37_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5069353.1"
                     /translation="MTGRNALSSGRGFRVQRTDSEDCTVLECFGRLTSDNVPLLKDAM
                     REACEKKVRIVLDLKEVPMMDSSGLGAVVTLHVSARTRGCSLEVVNASKQILELFSIT
                     NVLSLFESTGRCGRLM"
     gene            complement(1078..>1992)
                     /locus_tag="VII37_06190"
     CDS             complement(1078..>1992)
                     /locus_tag="VII37_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069354.1"
                     /translation="TEGTPSPAGGPCAHKAIKHGEPLPAAVGEPKGTPSPYPWAIPVL
                     RRDVATKPELRGHFDPHFADFKVDYPDQLRADEFLREFDGFVRARKANAKAQELPNYV
                     LVRLPNDHTGGTRPGFATPSASVADNDLAVGRVVDAVSHSPYWDDTAIFIVEDDAQDG
                     ADHVDAHRSIALVISKYAPGSTEHPHVEHGFFTTVSLVRTMEALLGLPPMNHNDAYAP
                     IMSVLFSGTGTQQPFQADYVNLHNGLIYQANAPTAAGAKASAAMNWRHADEANNQVLN
                     SILWRDRKGDVPMPPSQHTVFPAASGQR"
BASE COUNT          417 a          531 c          584 g          460 t
ORIGIN      
        1 ccagtctctt ccaccaaaaa tgctatctgt tgaaagctcg ggtgataatc tacgcctatc
       61 atcatgttcg gctccttcct gccgagcctc tggtcgtcaa gcaaccaaag tctactccgg
      121 tcgagggagc cgacactgtt aggcaatcaa gtgatttgag ggaagcagca catatttcgg
      181 caactcgatc tatcgggagt tgccatccta accgattggg gccctgagca agtttgaggg
      241 ctctgtgcag gaggacggaa atgaggtaca acgcattgcg cgtcgagaat gtgcaaccca
      301 gggaggggca gcatgacggg acgtaacgca ctgagttcgg ggcggggatt tcgagtgcag
      361 cggacggatt ccgaagattg caccgtcttg gaatgcttcg ggcggttgac aagcgacaat
      421 gtgccgctgc tgaaagacgc catgcgcgag gcctgcgaga aaaaagttcg aatcgtgttg
      481 gacttgaagg aagttccgat gatggatagt tcgggactag gggcggtggt gacgctgcac
      541 gtttcggcgc ggacgcgcgg ctgcagcctt gaagtggtga acgccagcaa gcagattttg
      601 gaattgttta gcatcacgaa tgtattgtcg ttgttcgaat ccacaggacg ctgcggcagg
      661 ttgatgtaaa tcttgcagtg tctgtggctc gacctggctg tgatccccca tgctccatta
      721 gaataacttg taattaatat acataaatag taaaataagg gatggttgcc acgaaagcat
      781 ccgcgccggc tagcgtcggt gaccgcaatt tcttccgtcc ttcttccagt tcgaacccgt
      841 ccgactgtcc cacgatcccg aaacactcta attccgattt ctcgagccgt gtctgcgagt
      901 caagtgattt gtattcagcg tcttgtgcac tttttgcagc gccgatcgtt tgttttcaac
      961 aagttgtgga ctcttttgcc aaaaacaggg gagtgggtag gggctctatg caaaaatcgc
     1021 gcgccgccca tcggagtggg gcgtcagtcg gctccgcgca gccagtcggt cgggtcctta
     1081 tctctgccct gacgccgccg gaaacacagt gtgctgggat ggcggcatgg ggacgtcgcc
     1141 tttgcggtcg cgccaaagga tggaattcaa aacttggttg ttggcctcat ctgcatggcg
     1201 ccagttcatc gcggcagatg ctttcgcgcc cgctgccgta ggcgcattgg cctggtaaat
     1261 gagtccgttg tgcaggttga cgtagtctgc ttgaaatggc tgttgcgtgc ctgtgcccga
     1321 aaacagcact gacatgatcg gagcatacgc atcgttgtga ttcattggcg gcaatcccag
     1381 cagcgcttcc atggtgcgaa ccaggctcac cgtggtgaag aaaccatgct ccacgtgggg
     1441 atgttcagtc gaacccggcg cgtacttgct gattaccagc gcgatgctgc ggtgcgcatc
     1501 cacgtgatcg gcgccgtcct gcgcatcatc ttccacaata aaaatcgccg tgtcatccca
     1561 gtaaggactg tgcgacaccg catcaacaac gcgacccacc gccagatcgt tgtccgctac
     1621 cgaagctgac ggagtggcga aaccaggtcg tgttccgcca gtgtgatcgt tgggcaagcg
     1681 taccagcaca taattcggca attcctgcgc tttggcgttt gctttccgtg cccgcacaaa
     1741 accatcgaat tcgcgcaaga actcatccgc gcgcaattgg tccgggtaat cgaccttgaa
     1801 atccgcaaaa tgcggatcga aatgcccgcg caactccggt tttgtagcga cgtcgcggcg
     1861 cagcaccggg atcgcccacg gataggggct tggtgttcct ttgggctcgc cgacggccgc
     1921 cggcaaaggt tcaccgtgtt ttatcgcttt gtgcgcgcag gggccgcctg ctggagatgg
     1981 cgttccttct gt
//
LOCUS       DATCBB010000566         1819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_310591, whole genome shotgun sequence.
ACCESSION   DATCBB010000566 DATCBB010000000
VERSION     DATCBB010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310591"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..777
                     /locus_tag="VII37_06195"
     CDS             172..777
                     /locus_tag="VII37_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5069355.1"
                     /translation="MKIRKNDQDEELLKRSAKGDEEAFTVLYRRHADALYRFAFRMTG
                     SAWGAEEIVQDVFMTLAREPGKYDAERGTLPAFLFGITRNKIMKHNERLPREISLVER
                     QEDGSGGGLTLRDSFTPAMWAEQRERLEKVRAAVMELPVEFRETVVLCELEEMSYEQA
                     ARMLDCPIGTIRSRLHRGRALLLAKLEMLRDAPRRWNSVAR"
     gene            796..1482
                     /locus_tag="VII37_06200"
     CDS             796..1482
                     /locus_tag="VII37_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069356.1"
                     /translation="MNCEEFEILGLDVQRDGSVDEDGRERALQHARVCGRCAALGASW
                     AAAQAELSALAESSRLLRVPARVESRVLQQFRLKHQPRQERKFLRLATWALAAAAVLV
                     CTLGIWDWHKWREGSLGANNTAAQDASGSAVSRSLTGSPETDANPVSDDSEILIAGNE
                     GNVGNDFTQLPGSSLQEVEDGAIVRVGMERASLAAFGLPVNEDEASDWIQVDLLVGSD
                     GSAQAVRLPE"
     gene            1534..>1819
                     /locus_tag="VII37_06205"
     CDS             1534..>1819
                     /locus_tag="VII37_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069357.1"
                     /translation="MKRNGWMNLVLGGVLVLSAGTAKAQGPGGGHPGPFGGPMELMGF
                     EGMHGGKLVKGAPFSATTTSTTQQTLQDGSTINRTGNGAIYRDSEGRSRRE"
BASE COUNT          401 a          451 c          589 g          378 t
ORIGIN      
        1 cgcggacgta acccaggaaa cccagactca ggccgcgccg tctaacatcc tgcgagcact
       61 caacttacct ttccatatga aatgccgcca aagccggccg aaaagccggg gaactttttg
      121 gtccttgcct gggaaatgct agatgaaacg cataatgcct gatctgaccc gatgaaaatt
      181 cgaaagaacg atcaggacga agagctcctc aagcgctccg ccaagggcga cgaagaagcg
      241 tttacggttt tgtatcgcag gcatgcggac gctctgtacc gctttgcgtt tcgcatgacc
      301 ggcagcgcct ggggagccga ggaaattgtt caggacgtct ttatgacgct agctcgcgag
      361 cctggtaaat atgacgctga acgaggcacg ctgcccgcgt ttcttttcgg catcacgcgc
      421 aacaagatca tgaagcacaa cgagcgcttg ccacgggaaa tttctctggt ggagcgccag
      481 gaagacggca gcggcggcgg gttaaccttg cgggattctt tcacgccggc catgtgggcg
      541 gaacaacgcg agcgcctgga aaaggtaaga gccgcggtga tggaactccc cgtcgaattt
      601 cgcgaaacgg tggttttgtg cgagttggaa gaaatgagtt atgagcaagc cgcgcgcatg
      661 cttgattgcc ccatcggtac gattcgttct cgtttgcatc gcgggcgcgc tttgttgctc
      721 gccaagctgg aaatgcttcg tgacgcgccg cggcgctgga actcagtagc acgatagtgg
      781 aaatggaaga ttgctatgaa ctgcgaagaa tttgaaattt taggtttgga tgtacaacgc
      841 gacggctccg tcgacgaaga cggccgggaa cgcgcactgc agcacgcgcg agtttgcggc
      901 cgctgcgccg cgctgggagc ttcatgggcc gcggcccaag cggaattatc tgcgttggcg
      961 gagagcagcc gattattgcg cgtgccggcc cgcgtggagt cgcgagtatt gcagcagttt
     1021 cgactcaagc accagcccag gcaagagcgc aagttcctgc ggcttgcaac ctgggccttg
     1081 gcagcggccg cggtcttggt atgcacattg gggatatggg actggcataa atggcgggaa
     1141 ggttcgcttg gtgcaaacaa cactgctgcg caggatgcga gtggcagcgc agtttcgcgc
     1201 tctttgacgg gttcgccgga gaccgatgcc aatccagttt cagatgattc tgagattttg
     1261 attgccggca acgaaggcaa tgtcggcaac gactttacgc agttaccggg gagttctttg
     1321 caggaagtgg aagatggcgc catcgtgcgc gtgggaatgg aaagagcttc gcttgccgca
     1381 ttcggtttgc cagtgaatga agatgaagcc agtgattgga ttcaggttga tttactggtg
     1441 ggcagcgacg gctctgcgca agcggtgcgt ctgcctgaat aaaagttttc tgcggtgatg
     1501 agtcaccgca ttgggagaga agaggagagc tgcatgaagc gaaatgggtg gatgaacctg
     1561 gttttgggtg gcgttttagt tttgagtgcg ggaaccgcta aggcgcaagg gcccggaggt
     1621 ggtcacccgg ggccgtttgg cggtccgatg gagttgatgg gctttgaagg aatgcatggc
     1681 ggaaagttgg tgaagggcgc gccgttctct gccacgacca cttcaacaac acaacagacc
     1741 ttgcaggacg gctcaaccat caatcgcacc gggaacggtg ctatttatcg cgatagcgaa
     1801 gggcgctcgc gccgcgaag
//
LOCUS       DATCBB010000567         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_408554, whole genome shotgun sequence.
ACCESSION   DATCBB010000567 DATCBB010000000
VERSION     DATCBB010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408554"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1383)
                     /locus_tag="VII37_06210"
     CDS             complement(94..1383)
                     /locus_tag="VII37_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5069358.1"
                     /translation="MVRTIGRWSLAALIVNSVIGGGVFGLPSVVAGRLGKYAPLAYLV
                     AAAGIALIAACLSEVASQFSETGGPYLYARTALGRFWGIQIAWMTWLSRLAAASGTAN
                     LFVTYLAEFYPRAVQPVYRVAILIALIGVLAIINYRGVRSATRVSDFLTSIKIFLLLI
                     FIGAGLGWLIAHGQVVPAPMTHDITSQDWLSTVLVLIFAFGGFEAVFFATGETRDPRK
                     DSAAALFYAMVLVTVIYTLVQVVVNGTLANPSASERPLAEAARHIFGNGAAAAIACAA
                     LVSIYGYLGANMLHTPRLTFALAERGDFPRFFAAVHPKFRTPHASILGYTVLLLVFAL
                     VGNFRWNITLSAVARLLTYASFAVALLVLRKRRPEADAFRLRGGRIIATLTLVFCVVL
                     FTQTPISNLSIVLGVIVIAALNWLLVRKRDATPRLEA"
     gene            1521..>2161
                     /locus_tag="VII37_06215"
     CDS             1521..>2161
                     /locus_tag="VII37_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5069359.1"
                     /translation="MSILPSATISYQSTMPSGSSIFPRNFRKDYPVAVRGEGCWIISA
                     DGRRFLDAAGQAAVINIGHGVPEIGRAMSEQASQIAFAHTSQFHSTPAEKLAARLLQL
                     APSNFQNGGRVYFTSGGSEATETAIKLARQYHLESGQPQRYGVISRRQSYHGSTLGAM
                     SVSGNVARRAPYQPLLAEWGHISECFAYHNAGNLKEEDYGLACANDLGKFLHGP"
BASE COUNT          547 a          651 c          541 g          422 t
ORIGIN      
        1 cgcgaacccg cgatcaagga atcctctcag ttgatccccg ggaatgtttc acggagaact
       61 caggatctaa agaaacagcc tcgccaattt ggactatgcc tctaatcgcg gagtcgcatc
      121 gcgcttgcgc accagcagcc agttcagtgc agcaattacg atgacgccga gaacgatcga
      181 caagttactg atcggcgtct gcgtaaatag caccacacaa aacactaatg tcagtgttgc
      241 gatgatacgg ccaccacgga gccgaaacgc atccgcttcc ggtctccttt ttcgcaatac
      301 caatagcgcc actgcaaatg aagcataagt cagcaaccgg gccacggcag aaagagtaat
      361 attccaccga aaatttccca ccagcgcaaa gactagcagt aaaacggtat aaccgagaat
      421 ggatgcgtgg ggagtgcgaa acttcggatg caccgctgca aaaaatcgag gaaaatctcc
      481 gcgctccgcg agcgcgaacg tcaatcgtgg cgtgtggagc atgttggcgc ccaaatagcc
      541 gtaaatggac accagcgccg cgcaggcgat cgctgccgca gccccatttc caaaaatgtg
      601 acgagccgct tcggcgagcg gacgttcgct cgcagagggg ttggcaagcg tgccgttgac
      661 cactacctgc accaaagtgt aaatcaccgt gaccagcacc attgcataaa acagcgctgc
      721 tgcagaatcc ttacgcgggt cacgcgtttc tccggtagcg aaaaaaacgg cttcaaatcc
      781 accgaaagca aaaattagta ccaaaaccgt gctaagccaa tcttgagagg tgatatcgtg
      841 ggtcattggt gccggcacaa cctggccgtg cgctatcaac cacccaagtc ccgcaccaat
      901 aaaaatcaat aaaagaaata tttttatgct ggtcagaaaa tcgctcacgc gcgtcgccga
      961 tcgcactccg cgatagttga ttatcgccag caccccgatc aacgcgatca aaattgccac
     1021 tcggtagaca ggttgcacag cacgtggata gaactccgca agataggtga cgaaaagatt
     1081 tgccgtgccg gacgccgccg ccagtcgtga taaccacgtc atccacgcga tttgaattcc
     1141 ccagaagcgc cccagcgccg ttcgcgcata gagataaggt ccacctgttt cgctgaattg
     1201 tgacgcgact tcgctcaggc acgccgcaat cagtgcgatc cccgcagcgg ccaccaaata
     1261 cgccagtggc gcatactttc ccaggcggcc tgcaaccaca ctgggcaagc cgaaaacacc
     1321 gccgccgatc accgaattta caattaacgc cgcgaggctc cagcggccga ttgtacgaac
     1381 caaacctccc gaagactcag ccgtcaatgc cgctccgtgc caagatttta attattagat
     1441 gagccgcgaa atcccaactc gccaaagtgc cgccagcgta gcacaactgc gaaaagcgct
     1501 ctcttgttga gctgtggctt atgtccattc ttccttctgc tacaatttcg taccaatcca
     1561 ccatgccgag cggctccagc atcttcccgc gaaattttcg aaaagattat cccgttgcag
     1621 tccgagggga aggctgctgg atcatttccg cagatgggcg tcgttttctt gatgcggccg
     1681 gccaagccgc cgtaatcaac atcgggcatg gcgtcccgga gattggccga gccatgtccg
     1741 agcaggcatc gcagatcgcc ttcgcgcata cttcgcaatt ccattccact cccgccgaga
     1801 aacttgccgc ccgtcttttg caacttgcgc cgtcgaattt tcagaatggt ggacgcgtgt
     1861 acttcacttc cggcggctcg gaagccaccg aaacggccat taagctggcg cggcaatatc
     1921 atctggaatc cggacagccg cagcgttacg gtgtgatttc gcgacgtcag agttatcacg
     1981 gcagcacact cggtgcgatg tctgtgagcg gcaacgtcgc gcgccgcgct ccgtatcaac
     2041 cattgcttgc cgaatgggga cacatctccg agtgcttcgc ctatcacaac gccggcaatt
     2101 taaaagagga agactacggc ctggcttgcg ctaacgacct gggcaaattt ctccatggcc
     2161 c
//
LOCUS       DATCBB010000568         1070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_51678, whole genome shotgun sequence.
ACCESSION   DATCBB010000568 DATCBB010000000
VERSION     DATCBB010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51678"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>1070
                     /gene="ribD"
                     /locus_tag="VII37_06220"
     CDS             91..>1070
                     /gene="ribD"
                     /locus_tag="VII37_06220"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY5069360.1"
                     /translation="MDANAIGYMQRAMSLARKAKGLASPNPLVGCVLVREGQIVGEGF
                     HQYDWRDHAEVVAIKSAGEKARGSTLYVTLEPCNHTGRTGPCTEAIIKAGIQRVVATI
                     DDPNPAVAGTGFDRLRAAGVEVFTGVLEEEARQLNESFAKWIRKRRPFVTLKSAMTLD
                     GQLALPQAGKRKSSTWITSEESRAEVHRMRHGSDALLTGIGTVLADDPLLTDRSGLPR
                     RRRLLRVILDSKLRLSPRARIVKTADDDLLVFTNEPFRSAKARNLQKAGVEIVSTKKR
                     AGHVDLQAVLKELGKREILSVLLEAGSALNGAALAADVVDKVFLFYAARFA"
BASE COUNT          258 a          259 c          337 g          216 t
ORIGIN      
        1 gccgattcgc ttcgtgggta ccggcgaaca gattaacgat atggttccgt tcgacgcgca
       61 aacctacgct gattccctct tcaactaatc gtggacgcca acgccatcgg gtacatgcag
      121 cgcgcgatga gcctggcgcg caaagcgaag ggcctggcga gcccgaatcc gttggtggga
      181 tgcgtgctgg tgcgcgaagg gcaaattgtt ggcgaaggct ttcaccaata cgattggcgt
      241 gatcatgcgg aagtcgtggc catcaaatct gcaggagaaa aagcacgagg ctcaacgctg
      301 tatgtaactc ttgaaccgtg caatcacacc gggcgcaccg gcccctgcac tgaagcaatc
      361 atcaaggcag ggattcagcg ggtggtggcg accatcgatg atccgaaccc cgccgtggcg
      421 gggaccgggt ttgatcgatt gcgagcggca ggagttgagg tatttactgg agtgctggaa
      481 gaagaagcgc ggcagctaaa tgaatcgttt gcaaaatgga ttcgcaagag gcgtccgttc
      541 gtcacgttga agtcggcaat gacgctcgat ggccaactag cgttgccgca ggcgggcaaa
      601 agaaaaagca gcacatggat cacgtcggag gaatctcgcg cggaagtgca ccgcatgcga
      661 catggttctg acgcgttgct cacgggcatt ggaacagtat tggcagatga tccgctcctg
      721 acggatcgca gcgggctgcc acgacgacga cgactgttgc gcgtaattct ggattcaaaa
      781 ttacggttgt cgccacgagc acgcatcgtg aaaaccgcgg atgatgatct gttggtattt
      841 acgaatgaac cgtttcgatc cgccaaggca cgtaatttgc agaaggctgg cgttgaaatt
      901 gtcagcacga aaaaacgcgc aggccatgta gatttgcagg cggtgctgaa ggagttggga
      961 aaacgtgaaa tcctgagtgt gctactggaa gcaggcagcg ctttgaatgg cgccgcgctg
     1021 gcagcagatg tagtggacaa agtgtttctg ttttacgcgg caagattcgc
//
LOCUS       DATCBB010000569         1031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_117077, whole genome shotgun sequence.
ACCESSION   DATCBB010000569 DATCBB010000000
VERSION     DATCBB010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117077"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VII37_06225"
     CDS             complement(<1..335)
                     /locus_tag="VII37_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069361.1"
                     /translation="MYAQEIRLNMASFWWSELSMQRADCIWLIMVMVAALAFSVWGLP
                     YFTQLVIQRGQSWFMRSSRFCLFMAAPIACAPAKLGRSSSDRHKTGEFAFPDMKKLSA
                     WEKSRPTLFR"
     gene            429..>1031
                     /locus_tag="VII37_06230"
     CDS             429..>1031
                     /locus_tag="VII37_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5069362.1"
                     /translation="MIRFGIAGFGMHAVKRLMPGFAKAQRCRVTALSRRDLQRAKESA
                     RQFEIDHAFASTAELCACPEVDAVFVSSPDGLHHYDVLEAIRHGKPVLCEKPMAMNPQ
                     EAREMVEAARAAEVLLGVAHVMRFEESVKFFRDHVAAGSIGRPLVARADFFAPMLTSA
                     RRWINDPSLATGGPLADLGVHCIDALRFIVGQEVRSVSALA"
BASE COUNT          220 a          287 c          306 g          218 t
ORIGIN      
        1 cgaaagagag ttgggcgcga tttttcccat gccgacaatt ttttcatatc gggaaaagcg
       61 aactcgcctg ttttgtgcct atcgctcgag cttcttccga gttttgctgg agcgcacgcg
      121 attggcgcgg ccataaataa acagaagcgc gaagaacgca taaaccagga ctgaccacgt
      181 tggatgacca gctgggtaaa atacggcagt ccccagacgc taaacgccaa agccgccacc
      241 attaccatga ttaaccaaat gcaatcggcg cgttgcatac taagttcgga ccaccagaaa
      301 ctcgccatgt tcaaccgaat ttcttgcgca tacacttgtg gacctccgat tagaaggccg
      361 ccatggaggt ggagcttgtc cgcgcaaaga aacttcacca accgctacgg aacgcaaagg
      421 acaaccgaat gattcgtttt ggcattgccg ggtttggtat gcacgcagtg aaacgcttaa
      481 tgccgggatt cgccaaggcc cagcgttgcc gcgtcacggc gttgtcacgt cgcgatttgc
      541 aacgggcaaa agagtctgcg cggcaatttg aaatcgacca cgcctttgcc tccactgccg
      601 aactctgcgc ctgtccggag gtggatgcgg tgttcgtctc cagtccggat gggctgcatc
      661 actacgacgt gctggaggct atccggcatg gcaagccggt gctctgcgag aaaccgatgg
      721 cgatgaatcc gcaagaggcg cgggagatgg tggaagctgc gcgcgccgcc gaagtcctgc
      781 tgggcgtggc ccacgtgatg cgctttgaag agagcgtgaa gtttttccgc gatcatgtgg
      841 ccgctggctc gatcgggcgg ccgctagttg cgcgcgccga cttctttgct cccatgttga
      901 ccagcgcacg taggtggatc aatgatccca gtctggccac cggagggcca ctggccgact
      961 tgggagtgca ttgcatcgat gcgctccgtt tcatcgttgg ccaggaggtg cgctcggttt
     1021 cagcgctggc g
//
LOCUS       DATCBB010000570         4589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_51805, whole genome shotgun sequence.
ACCESSION   DATCBB010000570 DATCBB010000000
VERSION     DATCBB010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51805"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..689
                     /locus_tag="VII37_06235"
     CDS             168..689
                     /locus_tag="VII37_06235"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY5069363.1"
                     /translation="MEELKKLIREVPDYPKPGILFYDLTTLLKDKRGFHTLIDRLCEH
                     YNGHTIDVVAGIEARGFIFAPALAYRLNAGFVPVRKPKKLPAKCASVSYALEYGTDTL
                     EIHEDAIKPGDRVIICDDLLATGGTAAATAKLIQQLGGKVEGLAFAVELTFLNGRSKL
                     PGQDVFSLIQYDK"
     gene            complement(787..1539)
                     /gene="rlmB"
                     /locus_tag="VII37_06240"
     CDS             complement(787..1539)
                     /gene="rlmB"
                     /locus_tag="VII37_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY5069364.1"
                     /translation="MSPLIDRLTGINAVREALQAGSAIDHVVIARGRQDTRTEEIVQL
                     ARGRGIPVRFEDRSQIDRLANTKDHQGVVALAAARAAGSLEDILANAKAGGHGGLIVL
                     LDGVEDPHNLGAIIRTALAAGAQGVVIPERRAVGLTDTVARASAGALAHLPIARVTNL
                     ARAMEELKEAGYWLIGLDEAADKTYTQVDYTSPVGIVLGGEGKGLHELTRKRCDFVVS
                     LPTSGPVKSLNVSVAAGVVLFEALRQRAGKTK"
     gene            complement(1603..2097)
                     /locus_tag="VII37_06245"
     CDS             complement(1603..2097)
                     /locus_tag="VII37_06245"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069365.1"
                     /translation="MDIWEILLQRRWTADEKFSPELQSWCERAAAMLGSHVADRAALG
                     DLLDLIFQYDAEKVLERVESHAVLARYGSREVVRRLALLLLDGGPLDSERFKEIITIM
                     KEASDLRSRDLFHPIRLALAGKTGEGELDRVILLIDEAAALPFAVPVKSVRARILEFC
                     SALD"
     gene            complement(2232..2711)
                     /gene="ispF"
                     /locus_tag="VII37_06250"
     CDS             complement(2232..2711)
                     /gene="ispF"
                     /locus_tag="VII37_06250"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY5069366.1"
                     /translation="MSVRCGIGYDLHRLEAGRKLIIGGLEVPFDKGPAGHSDGDVLAH
                     ALCDALLGAAGLGDIGTHFPDTDPKWKGADSMQFLEHAAQLLDDKAFSIEHVDAVVIL
                     ERPKLGPHFPKMREALAKALGVMADRIHLKAKTNEGVDAVGRGEAIAAYVVATLETT"
     gene            complement(2708..3424)
                     /gene="ispD"
                     /locus_tag="VII37_06255"
     CDS             complement(2708..3424)
                     /gene="ispD"
                     /locus_tag="VII37_06255"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY5069367.1"
                     /translation="MGRITAILPASGLGTRMGAETPKQFLELDGTPIVIHSLRRIASC
                     PLVTDIIVATRADVMESLQSKIQAEKFTQSVRMVRGGDSRQDSVAQALREVPADTEIV
                     LVHDAVRPFVTVEQITRVIEEARKCKAAILGIPAMDTVKEVKRASLPEDVALIIGTIP
                     RERVVLAQTPQAFSAKLLKEAFARAEADGVNASDEAGLVERIGHDVHVVLGSERNIKI
                     TRPADMELARFYLERERSKK"
     gene            complement(3550..>4589)
                     /locus_tag="VII37_06260"
     CDS             complement(3550..>4589)
                     /locus_tag="VII37_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5069368.1"
                     /translation="LAHASMGGLIGGACGALAGMGAALLLTLIISYTSQPASTKSFLD
                     FISLLAFSYLGMTTGFRCGEQWRFSGPKAEASSESPANGLMKLLDTSVLIDGRIAEIC
                     EAHFLDGVLAVPQFVLHELQLVADSSDPLKRQRGRRGLEVLQRIQKMPQVEVRVVDDD
                     FPHTTDVDHKLIELARRTGAKIVTNDFNLNKVATVQGFAVLNVNELANALKPAVLPGE
                     TMHVLIQREGKEAMQGVAYLSDGTMVVVDGARRMINKSVDIIVTSVHQTTAGKMIFGR
                     VDERVGPIGPFARAVAAGRGVSGNSTASYSAGSNAAREEDSNRATDSNGGDGRAADQT
                     TSGMAPQTDVD"
BASE COUNT         1038 a         1499 c         1091 g          961 t
ORIGIN      
        1 aaagaagtcc gtgaagaagt ggccacaccc gatccacagt acgaccaggt atttgtaata
       61 ggccgcggcg attgaaacat gaacatctaa taaggattcg cgtaataacg atccgcctgc
      121 tgcggcctta cctcaattaa accggaacga ctacaggagc cctgcgaatg gaagaattaa
      181 agaagctgat ccgcgaagtg cccgactatc cgaaaccggg gatccttttc tacgacctga
      241 ctacgctcct gaaagataag cgcggtttcc acactctgat cgatcgcttg tgcgaacact
      301 acaacggtca caccatcgac gtcgtcgccg gaatcgaagc ccgcggtttt atttttgccc
      361 ccgcattagc ctatcgcctc aacgccggtt ttgttcccgt gcgcaaacct aagaaacttc
      421 ccgccaaatg cgccagcgtc tcctacgctt tggaatacgg caccgacacg ctggaaattc
      481 atgaggacgc catcaagccc ggcgatcgcg tcataatctg cgatgatttg ctggccaccg
      541 gcggcaccgc tgcggctacc gccaaactca ttcagcaatt gggcggcaaa gtcgaaggct
      601 tggccttcgc cgtagaatta acattcctga atggccgctc gaaactaccc ggccaagatg
      661 ttttttcttt gatccaatac gacaaataat tccagctcag cggcacgcta gctgacaact
      721 gggcaactgg acttgctcta gcaacgagga atcagttttt ccttctccga ctttttcttc
      781 cgcaggctat tttgttttcc ccgcgcgctg ccgtaaggcc tcaaacaaaa ccacccctgc
      841 ggccacggat acgttcagcg atttcactgg accgctagtc ggcagtgaaa caacaaaatc
      901 gcaacgtttg cgcgtcaact catgcaagcc tttgccctcg cctcccaaaa caattcccac
      961 aggcgaagtg taatcgacct gcgtataggt tttatcggcg gcctcatcca gcccgatcag
     1021 ccagtaaccg gcttccttca attcttccat ggcgcgggcc aggttcgtaa cccgcgcaat
     1081 cggtaagtgc gccaacgccc ctgccgacgc gcgcgccacg gtatccgtca agcccaccgc
     1141 gcgccgctcc ggtatcacca cgccttgcgc acccgctgcc aatgctgttc gtataatggc
     1201 gccgagattg tgcggatctt ccactccatc caacaacaca atcaacccgc cgtgcccgcc
     1261 ggcctttgcg ttcgcgagaa tatcttccag cgaacccgca gctcgagccg ctgctaacgc
     1321 cacaacccct tgatgatctt tggtgttggc cagccggtcg atttggctgc gatcttcaaa
     1381 ccgcactgga atcccacgtc cgcgcgccaa ctgcacaatt tcttccgtgc gcgtgtcctg
     1441 ccgcccgcgc gcaatgacta catgatcaat cgcgctaccg gcttgcagcg cttcacgcac
     1501 cgcgttaata cccgtcaatc ggtcaatcag aggactcatg cgagcagaca gtgtacaagc
     1561 cgctgcaaat cgaagaaagc accaggccaa aattaagcgt acttaatcaa gtgcggaaca
     1621 aaattccagt atccgtgcac gcacggattt caccggcacc gcaaaaggca acgccgccgc
     1681 ttcatcaatc agcaaaatca cgcgatccag ttctccttcg ccggtcttgc ctgccaacgc
     1741 cagtcgaatc ggatgaaaca aatcccggct ccgcaaatct gaagcttctt tcatgatcgt
     1801 gattatttct ttgaatcgtt ccgagtccag cggtccaccg tcgagcaaca acaaagccag
     1861 ccgccgcacc acttctctgg acccataacg cgccagcacc gcatggcttt ccacgcgctc
     1921 cagcactttc tcggcgtcat attgaaaaat caaatctagg aggtcaccga gtgcggcgcg
     1981 gtcggcgacg tgtgatccaa gcatggccgc cgcacgttcg caccacgact gcaactctgg
     2041 agaaaacttt tcgtccgcag tccatctgcg ctggagcaaa atctcccaaa tgtccacgcc
     2101 ggtaaaaaga ttgggcgcgg caattgaatc ccgcgttcga cgctcagagt tcatgtggga
     2161 ggaagtctac agtaggcaaa taggagttcg cggcagcgta caggatcagc aatgattcag
     2221 acgatacaac atcaggtagt ctccagcgtg gccaccacat aagccgcaat agcctcgccg
     2281 cgtccgaccg catcaactcc ttcattcgtt ttggccttca aatgaatgcg atccgccatg
     2341 acgccaagcg ccttcgccaa cgcctcgcgc atcttgggaa agtgtgggcc caatttaggc
     2401 ctttcgagaa tgaccaccgc atcgacatgc tcaatcgaga atgccttatc gtctagcagc
     2461 tgcgccgcgt gctccaaaaa ctgcatgctg tcagcgcctt tccacttcgg atcggtatca
     2521 ggaaaatgcg tgccgatatc gcccagtccg gccgctccca aaagtgcatc gcaaagcgcg
     2581 tgggccaaaa catcgccgtc cgaatgcccc gccgggcctt tatcaaacgg cacttcaagc
     2641 cccccaataa ttaatttgcg ccccgcttcc aggcggtgca ggtcgtaccc gatgccgcag
     2701 cgcacgctca ctttttgctg cgctcgcgtt ccaggtaaaa ccgcgccaac tccatatccg
     2761 cgggcctggt aattttaatg tttcgctccg aacccaacac cacgtgcacg tcatgaccga
     2821 tccgttccac caggcccgct tcgtccgagg cattcactcc gtcggcctcc gcgcgcgcaa
     2881 acgcctcctt cagcagtttc gcggagaacg cttgaggggt ttgcgccagc accacacgct
     2941 cgcgcggaat cgtcccgata atcagcgcca cgtcttccgg caaactggcg cgcttcactt
     3001 ccttcaccgt atccatcgcg ggaatgccca ggatggccgc cttgcacttc cgcgcctctt
     3061 caatcacacg agtaatttgc tccaccgtaa caaatggccg caccgcatcg tgcaccaata
     3121 caatttccgt atcggcaggc acttcgcgca acgcctgcgc cacggaatcc tgccgcgaat
     3181 ctccgccgcg caccatacgc acagattgcg taaacttttc cgcttgaatc ttactttgca
     3241 gcgattccat tacgtcagcg cgcgtggcca caataatgtc tgtgaccagc gggcacgatg
     3301 caatgcgccg caggctgtga ataacgattg gcgttccgtc gagctccaga aattgcttgg
     3361 gcgtctccgc gcccatgcgc gtacccagtc ccgacgccgg aagaatggca gtgattcgtc
     3421 ccattttatc gtttgaacgt gtggggcccg tctttagatg aacgatcaga cgaacgatcc
     3481 gatgaacgaa caattatata ccgagcggaa ggttgcagcg tgctttgaca cttccggggc
     3541 aactacgcgc taatcgacgt ctgtttgtgg ggccatgcct gaagtagtct ggtccgccgc
     3601 gcggccatcg ccgccattgc tatcagtagc gcgattactg tcctcttctc gcgccgcatt
     3661 tgatcctgcg ctatacgatg ccgttgaatt accgcttacc ccgcgtcccg ctgccacagc
     3721 ccgcgcaaaa ggaccaatcg gccccacgcg ctcatccaca cgcccgaaaa tcatcttgcc
     3781 cgcggtcgtc tgatgcaccg aggtcacgat aatatccaca cttttattga tcatgcgccg
     3841 cgcgccgtcg accaccacca tggtcccgtc gctcaaatac gccacgccct gcatggcttc
     3901 cttgccttcg cgctgaataa gcacatgcat cgtttcgccg ggcagcactg ccggctttag
     3961 cgcattggcc aactcattca cgttaagcac cgcgaatccc tgcaccgtgg ccactttatt
     4021 caaattaaaa tcattcgtta cgattttcgc gccggtacgc cgcgccaatt caatcagctt
     4081 gtggtccaca tcagtggtat gcggaaaatc gtcatccaca acgcgcactt ccacctgcgg
     4141 cattttctga attcgctgca acacttccag cccgcgccgt ccgcgttgcc gcttaagagg
     4201 atcggaggaa tcggccacca gctgcaattc atgcagcaca aattgcggca ccgccagcac
     4261 tccgtccaga aagtgggctt cacaaatctc agcgatgcgc ccgtcgatga gtacgctggt
     4321 atccagcagc ttcatcagcc cgttcgcggg actctcgctg ctcgcttccg ctttaggccc
     4381 cgaaaaacgc cactgttctc cgcaacgaaa gccggtcgtc atacccaaat aactgaacgc
     4441 cagcaacgag ataaaatcca ggaacgactt ggtggatgcc ggttgcgagg tgtaagagat
     4501 gattaacgtc agcaataatg ccgcgcccat tccggccaag gctccgcagg cgcccccaat
     4561 caagccgccc atgctggcgt gcgccaaac
//
LOCUS       DATCBB010000571         1282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_278438, whole genome shotgun sequence.
ACCESSION   DATCBB010000571 DATCBB010000000
VERSION     DATCBB010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278438"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VII37_06265"
     CDS             complement(<1..1014)
                     /locus_tag="VII37_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5069369.1"
                     /translation="MPDQKPLTILCVTSYEKGQEFIRTCKGMGCRVLLLTVDKLKDGD
                     WPRECLDGTFTMPEDLPLQGLIYSVSYIARSQRIDRIVALDEFDMENVSALREHLRIP
                     GMGLTTVRYFRDKLSMRARAKEAGIRVPEFVPVLNYDDLREYMARVPAPWLLKPRSQA
                     SGIGMKKIQSPEELWPWLDELGDQQSFYLLEHFVPGTVYHVDSIASEREVVFAEAHKY
                     GTPPLTTSHQGGVFTTSTLPADAPEYSELLSLNQKVLGELGFVRGVTHAEFLRADSDG
                     KFYFIEVAARVGGAFISDVIEAATGINLWREWARLEVGAGREAYQLPAHKREYAGVIL
                     SLAR"
BASE COUNT          299 a          412 c          305 g          266 t
ORIGIN      
        1 gcgcgccagc gacagaatta cccccgcgta ttcacgcttg tgagccggaa gttggtaagc
       61 ctccctgccc gctcctacct ccaaacgtgc ccattcgcgc cataaattaa tgcctgtggc
      121 cgcctcgatc acgtcgctga taaatgcccc gcccacacgc gctgccacct cgataaagta
      181 gaacttgccg tccgaatcgg ctctcagaaa ttccgcatgc gtcacgccgc gaacgaaacc
      241 caattcgcct agaacttttt gattgagcga aagcagctcc gagtattccg gcgcatctgc
      301 cggcaatgta ctggtggtga agaccccgcc ttggtgtgag gtcgtcaatg gcggcgtgcc
      361 atatttatgc gcctccgcaa aaaccacctc gcgttcggac gcaatgctat ccacgtgata
      421 caccgtgccc ggaacaaagt gctccagcaa atagaaagat tgctgatcgc ccaactcatc
      481 aagccacggc caaagctctt ccggcgattg aatcttcttc atgccgatgc cggaagcctg
      541 cgagcgcggc ttcaagagcc acggcgcagg cactcgcgcc atgtactcgc gcaggtcatc
      601 gtaattcagc accggcacaa attccggaac acggatgccc gcttccttgg cccgcgcgcg
      661 catggaaagc ttgtcgcgaa aatagcgcac agtcgttaac cccatgcccg ggatccgcaa
      721 atgctcccgc agcgcggaca cgttctccat atcgaattcg tcgagcgcca cgatgcgatc
      781 gatgcgttgc gagcgagcga tgtaactcac cgaataaatc aagccctgca gcggaagatc
      841 ctcaggcatg gtaaacgtgc catcaaggca ctcccgcggc cagtcaccat cctttaattt
      901 gtccacggta agcagcagca cgcgacaacc catgcctttg cacgtgcgaa taaactcctg
      961 tcccttttcg taggaggtta cgcaaagaat tgtcagcggc ttctgatccg gcataaacac
     1021 cctgcaactt ttatggggta cctttaaccg cgcgatgata gatcacataa attctagatt
     1081 catcgagcca cgcagccggg cggattatac ttttttatct accgccggaa acagaaattg
     1141 caacaccggc ccaacacgct gtgaccatgc cgcttcgttg tgttccgcgc cttccactcg
     1201 ctcgtagtgc agatcaccat ccagctgcca cccttttttc accagcacgt cccggaaatt
     1261 ctcaacgtca cggaggatct gc
//
LOCUS       DATCBB010000572         1564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_440309, whole genome shotgun sequence.
ACCESSION   DATCBB010000572 DATCBB010000000
VERSION     DATCBB010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440309"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VII37_06270"
     CDS             complement(<1..737)
                     /locus_tag="VII37_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5069370.1"
                     /translation="MIARPPEMGAVRSPAEASRANNELRLSVEQVSYAYSPNPTQAPL
                     FTLEATSFQARAGEIVAILGPNASGKSTLLKLIAGTLEPLSGRVLLNGFVTHSLNART
                     RAQRIAMVQQESPLLFPARAWEFVLQGRHPHGRALRFESATDIAVARNALAQVGAAQL
                     SDRWMAELSGGEKQRVILARALAQQPLLLLLDEPTLHLDIAAQVDLLHGLRHLAVENR
                     YTVVVVTHELNLAAEYADQVVLMQRGK"
     gene            complement(740..>1564)
                     /locus_tag="VII37_06275"
     CDS             complement(740..>1564)
                     /locus_tag="VII37_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5069371.1"
                     /translation="LGIFVGASLSVAGTGFQALLRNPLADPYVLGVSNGAALGAILAL
                     LVSPHLALLPAVADLLTPLGAFLGAAATIAAVYFLGRREGQIDSTTLLLGGVITGSFL
                     AAIIMFLMTSETGSNLRGMVYWLMGNLSTSLQPSVYWVLWIGFLLGAGAIYTTASDLN
                     LLLSGEKEAMHLGVDLPRVRLVVYIAASLLTGLAVSVSGSIGYVGLLVPHIMRQIFGS
                     DHRVLLPTAAFGGAIAVVIADTIGRTVVPPAELPVGAMTALAGAPLFIYLLRRRMA"
BASE COUNT          395 a          559 c          357 g          253 t
ORIGIN      
        1 cacttcccgc gctgcatcag caccacctgg tccgcatatt ccgccgccag atttagctcg
       61 tgcgtcacca ccaccaccgt gtagcgattt tctaccgcca gatggcgcaa tccgtgcagc
      121 aaatccacct gcgccgcaat atccaaatgc agcgtcggtt catccagtaa caaaagtaac
      181 ggttgctgcg ccaacgctcg cgccagaatc actcgctgct tctcgcctcc agacaattcc
      241 gccatccaac ggtcacttag ttgtgccgcg cccacttgcg ccagcgcatt tctcgccacc
      301 gcaatgtccg tcgcactttc aaaacgcagc gcccgcccgt gcggatgccg cccctgaagc
      361 acaaactccc atgcccgcgc gggaaacagc agcggactct cctgctgcac catggcaatg
      421 cgttgcgcgc gcgtccgtgc gttaagcgaa tgcgtgacga acccattcag cagcacccgc
      481 cccgaaagcg gctccagcgt gccggcaatc aatttcagca acgtcgattt gccgctcgcg
      541 ttcggcccca ggatggccac aatctcgccc gcccgcgcct gaaagctggt cgcttccaaa
      601 gtgaacagcg gagcctgcgt cgggttaggc gagtacgcat aactcacctg ctccacactc
      661 aagcgcaact cgttattcgc acgcgatgct tccgcaggcg agcgcacggc ccccatttca
      721 ggcggccgcg caatcatgtt cacgccattc tccggcgcaa caaataaata aaaagcggag
      781 cgcccgccaa cgctgtcatc gctcccaccg gcaattccgc gggaggcacc accgtgcgcc
      841 caatcgtgtc cgcaatcacc accgcgatcg ctccgccaaa cgcagccgtt ggcagcaaaa
      901 cacggtgatc cgatccaaaa atctgacgca tgatgtgcgg caccagcaaa ccaacataac
      961 caatcgaccc gctcaccgat actgccaagc ccgtgagcag cgatgccgcg atataaacga
     1021 ccagccgcac ccgcggaaga tcgacgccca gatgcatggc ttctttttcg cccgacaaca
     1081 acaaatttaa atccgaagcc gtcgtgtaaa tcgctccagc gcccaataaa aatccaatcc
     1141 acaaaaccca atacacactg ggttgcaacg acgtggaaag attccccatc agccaataca
     1201 ccattccgcg caaattgctg cccgtctccg aggtcatcag aaacataatg atcgcggcaa
     1261 gaaatgagcc agtaataaca ccgccaagca gcagcgtcgt actatcaatc tgcccttcac
     1321 gcctccccaa aaaataaacc gcggcaatcg tagccgctgc tcccaaaaaa gctcccagcg
     1381 gcgtgagcaa atccgccact gcaggcaaca aggccaaatg cggagaaacc agcaacgcga
     1441 gaatcgcgcc cagggccgcc ccattcgaaa ctcccagtac ataaggatct gcgagcggat
     1501 tgcgcagcaa cgcctgaaat cccgtgcccg ccaccgacag cgacgcaccc acaaaaattc
     1561 ccag
//
LOCUS       DATCBB010000573         1028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_343303, whole genome shotgun sequence.
ACCESSION   DATCBB010000573 DATCBB010000000
VERSION     DATCBB010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343303"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VII37_06280"
     CDS             complement(<1..897)
                     /locus_tag="VII37_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069372.1"
                     /translation="MVRDKFGQMDFNSTWGGMAQKFLKQLESGKDMGGYINSDLAEVH
                     TAQEHGINKIELQSRPFAQTDWVAMLTADYQKNWPYLWFVVRKWCAFKLLPPFPFVNG
                     KPAFIAALDLPGNEIPKDNCATLVNGCPESWTALAELMRFDSRTGQSPVVDFLIAQKI
                     VGKKLLKLYHDHAGSNTHEFRRVIYREMSLHQSGDASFIPAPRGGDSELIAAGHLALS
                     GEYVWEELRGVAKQFSVGKGMPQPNRCVAVDCGYAEKFNREVLRKCYESATVYKFYDP
                     MSRNRPALFYRQPIHNYCQPCPA"
BASE COUNT          211 a          290 c          290 g          237 t
ORIGIN      
        1 cgccgggcag ggctggcaat agttgtggat aggctgccga tagaacaacg ctggccggtt
       61 gcggctcatg ggatcataga atttatagac ggtggcgctc tcgtaacatt tcctaagtac
      121 ttcgcgatta aatttttccg catacccgca atccacggcc acgcaacggt tcggctgcgg
      181 cattcctttg cccacggaga attgtttggc cacgccgcgc agctcttccc acacgtattc
      241 accgctcaat gccaggtggc cagcggcgat gagttcgctg tcgcccccac gcggcgctgg
      301 gatgaagctg gcatcgccgg attgatgcag gctcatctcg cgatagatca cgcgacgaaa
      361 ttcatgagtg ttggacccgg cgtggtcatg gtatagtttc aggagcttct tgccaactat
      421 tttttgcgcg attaaaaaat ccacgacggg cgactgaccg gtgcgcgaat cgaaacgcat
      481 cagctcggcc agggccgtcc acgattcggg gcagccattg accagggtcg cgcaattgtc
      541 cttgggtatt tcgttcccag gcaaatccag cgcggcaatg aacgcgggtt tgccatttac
      601 aaacgggaac ggtggcagca gcttgaatgc gcaccatttg cgcaccacga accataggta
      661 aggccagttt ttttgataat ccgcagtcag catggccacc cagtcggttt gggcgaacgg
      721 gcgggattgg agttctattt tgttgatgcc atgctcctgt gcggtgtgca cctcggccag
      781 atcggagttg atgtagccgc ccatgtcttt gccagactcc aattgcttca aaaacttctg
      841 agccatgccg ccccaggtgc tgttgaaatc catctggccg aatttatcgc gcaccatcgg
      901 cgcgtaatag ctcggcagat gataaccgcg gtgaaatttc tcgcccgcgc gtgtcggaat
      961 ccaccttcca tgcttatcca gccagaggcg gtgttcgtcc cggacatggc cgccgcaatg
     1021 cggacatt
//
LOCUS       DATCBB010000574         4289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_180976, whole genome shotgun sequence.
ACCESSION   DATCBB010000574 DATCBB010000000
VERSION     DATCBB010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180976"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VII37_06285"
     CDS             <1..1406
                     /locus_tag="VII37_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5069373.1"
                     /translation="GRVLKIAEASLIFCVTAAVLAFGGTEPISFAVVEIVLLGAVLAV
                     LVVSKDFEWVGSLRRAAVPVSLITLVMFQLVPLPVVLVRLLRPDSSTFGANLPGSDHH
                     SFSRLSIAPYNTRNHLILLICCVTVFFFARMVAQDRASRRRIVTWLVALGTFEALYGL
                     VQYLTGWQRIFGYVKKYNLEEATGTYINRNHFAGFLEMMIPFGVALVLYENAKLPRKA
                     VPGRDVNARIKRVLGGRKLSRIGLWLLAAMVMVAGLFFSLSRMGIISAVASLAVMAAF
                     AGFQRKAGLWVAGGIMACGIILVLWMGAAPMLERFGTIGEEYASANESRWSLWKDTAR
                     LVGGHPLLGSGLGTFPVAFTRVQSTFLGQFVNHAHNDYLELASDLGIPMAAVFFGSTG
                     ALLVRVARKAASSEVSFERAMALGCLGSIAAILLHSLADFNLYIPANALVFSLILGLA
                     ASIPATNSGVLRRANEA"
     gene            1396..2013
                     /locus_tag="VII37_06290"
     CDS             1396..2013
                     /locus_tag="VII37_06290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY5069374.1"
                     /translation="MRRELQLSAKRIMDVVLAILGLIVFVPLLLVCAIAIKLNSRGPV
                     CFRLPVAGLRGKGFDQIKLRTMVHGAREIGDRFETSLCDPRITRVGHFLRRWSIDELP
                     QLWNVLRGEMSIVGPRPAFFEIASKYSVDQARRLAMRPGLTGLAQVQGRNSLPWPERV
                     ELDIFYVEHHSLRLDCEIIARTIPVLFQGEGVYGKDGRVRVQDLG"
     gene            2021..2692
                     /locus_tag="VII37_06295"
     CDS             2021..2692
                     /locus_tag="VII37_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEY5069375.1"
                     /translation="MGNKRVLVWGAGGHGKVVIDALMASGEWKVVAVLDEDEKKRGTE
                     VLGVKVILLNGDVAEAARGSDCDRVAIAIGDNYVRLEKLRQARRAGLRAVNVVHPKAH
                     ISRFVKMGEGVTILAGATINPGTTIEDNVCVNTSASVDHDNYLSESCHIFPNATLTGG
                     VRIGEFAYVGSGAVIAPNLTVGKYSCVGAGAVVLADVAEGKVVFGVPAKVRGEQTKRP
                     GKENS"
     gene            2689..3879
                     /locus_tag="VII37_06300"
     CDS             2689..3879
                     /locus_tag="VII37_06300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5069376.1"
                     /translation="MSVADPASVETEKHTPAFSKLLPMVVPEGLPGEEFLEEVRGILA
                     SKQLTNGAHVRKFEEAAAEYLGVAHCVAVSSCTAGLLLVLKALDLRGEVILPSFTFHA
                     TAHAVAWNGLTPVFADCDAKTFCVASKAVRAQISSRTAAILVVHLFGNPAAVRELEEI
                     AVELQVPLIFDAAHALGSSTGGRRIGRFGTAEVFSFSPTKLVVAGEGGLITTRDAGLA
                     EKFRAARNYGDAGNYDPEILGVNARMTEINAAMALHGLAGLEARIERRNEIRTRYERN
                     LEDIAGLRFQEISEGGRSTCKDCSVIADQREFGHSRDWLVELLHRENIQARKYFWPPV
                     HRQKLYSAIWDGRPLPVTDMISDGVVSLPIYSSLRDESVDKVCEVIRRAHRTENSKWA
                     TGAA"
     gene            3882..>4289
                     /locus_tag="VII37_06305"
     CDS             3882..>4289
                     /locus_tag="VII37_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5069377.1"
                     /translation="MKTKPSVDGEANRRVLVLGGAGYLGSVLVRQLLEKGRRVRVLDS
                     FMFGEQALDGVRCHPNLEVVRGDVRDVGMVVRCMRNCDAVIHLAGIVGDPACEENKEL
                     AVEVNRAATRMLANVARECGVRRFIFASSCSVYG"
BASE COUNT          902 a          886 c         1471 g         1030 t
ORIGIN      
        1 gtgggcgagt gctgaagatt gccgaggctt cgctcatttt ttgcgttacg gcagcagtat
       61 tggcattcgg cggcaccgag ccaatttctt ttgcagtcgt ggagatcgtg ttattgggcg
      121 cggttttggc cgtgcttgtt gtaagtaaag attttgaatg ggtgggatct ttgcgaaggg
      181 cagcagtgcc cgtttctttg attactttgg tgatgtttca actggttcca ttacctgtgg
      241 tgttagtgcg gttgttgcgc ccggatagtt ccacatttgg cgcgaatcta cccggaagcg
      301 atcatcattc cttttcgcgt ttaagcatcg ctccttacaa cacgcggaac catttgatcc
      361 tgttgatttg ctgcgtgacg gtattttttt tcgcacgcat ggtggcgcag gatcgcgcaa
      421 gtagaagacg aatcgtgaca tggctggtgg ccctgggaac gttcgaagcg ctttacgggc
      481 tagtgcagta tctgaccggc tggcaaagaa tttttgggta cgtaaaaaaa tataacctcg
      541 aagaggcgac cggaacgtac attaaccgga atcactttgc gggattcctg gaaatgatga
      601 ttccgttcgg cgtggcgttg gtgttgtatg agaatgccaa gctgccgcga aaagctgtgc
      661 ccgggcgaga tgtaaatgcg cggatcaaga gggtgctagg aggtagaaag ctttcgcgaa
      721 tcggattgtg gttgctcgcg gctatggtga tggtagcggg attgtttttt tcactttcgc
      781 gcatgggaat tatttcggcg gtagcttcgc tagcggtgat ggcggcattt gctggattcc
      841 agcggaaggc cggactatgg gtcgctgggg ggattatggc ctgcgggatc attttggttc
      901 tgtggatggg agcagcgccg atgctggagc gcttcggaac cattggcgag gaatatgcca
      961 gcgcgaacga gagtcgctgg tcgctctgga aagataccgc gcgactggtt ggaggtcatc
     1021 cattgctggg aagcgggttg ggaacttttc ccgtggcgtt tacgagagtg cagagcacgt
     1081 ttctcggaca atttgtgaat cacgcgcaca acgactacct ggaattggcg agtgatttag
     1141 gaataccgat ggcagcggta ttttttggat cgacgggcgc gcttctggta cgcgttgcgc
     1201 ggaaagccgc ttcatcggaa gtgagtttcg agcgagccat ggctttaggc tgcctgggga
     1261 gcatcgcagc gatactgctg cacagcttgg cggattttaa tctgtacatt cctgcgaacg
     1321 cgctggtgtt ttcgttgata ttaggattgg ccgcttcgat ccctgctact aactcaggcg
     1381 tgctcaggcg ggccaatgag gcgtgaattg caacttagcg ctaaacgaat tatggacgtg
     1441 gtgctggcga tcctggggct aattgttttc gtgccactgc tcttagtttg cgcgatagcg
     1501 attaagctca attcccgtgg gccggtgtgt tttcgattgc ctgtagcggg actacgcggg
     1561 aagggcttcg atcaaataaa actgcggacc atggtgcacg gagcgcgaga aatcggagat
     1621 cgctttgaga cgtccttgtg cgatccacga attacccgcg tgggacattt tttgcggcgc
     1681 tggagtatcg atgagttgcc acaactgtgg aatgtgctgc gcggagaaat gagtatcgta
     1741 ggaccgcggc cggcgttttt tgaaatagcg tcgaaatatt ctgtggacca ggcgcggcgg
     1801 ctggcaatgc ggccgggtct gacgggattg gcgcaggtgc aagggcgaaa ttcactgccg
     1861 tggcctgagc gagtggagct ggatattttt tacgtggagc atcattcgct gcggctggat
     1921 tgtgagatca tcgcgcgcac cataccagtt ttatttcagg gcgaaggcgt gtacggcaag
     1981 gatggccgag tccgcgttca ggatttaggg tagtcgcgac atgggaaata aacgggtgct
     2041 ggtgtgggga gccggcggcc acggaaaagt ggttatagat gcgctgatgg ctagcggtga
     2101 atggaaagtt gtggcggttt tggatgaaga cgagaaaaaa cgcggcacag aagttttggg
     2161 cgtgaaggtg attttgctta acggtgacgt ggcggaagca gcgagaggat cggactgcga
     2221 cagagttgcg attgcgatcg gcgataacta cgtgcgttta gaaaaactcc ggcaagcgcg
     2281 gcgcgccggg cttagggcgg tcaatgtcgt acatccgaaa gcgcacattt cgcgttttgt
     2341 aaagatgggc gagggcgtga cgattctcgc gggagcgacg ataaatccag gcacaacaat
     2401 tgaggacaat gtttgcgtga acacttcggc gtcggtggat cacgataatt atttgagtga
     2461 aagctgccat atatttccca acgctacgct gaccggtgga gtgcgcattg gagagtttgc
     2521 ctatgtggga tcgggggcgg tgatcgcgcc gaatcttacc gtcgggaaat atagctgtgt
     2581 gggcgctggc gcggtggtgc tagctgatgt cgcagagggc aaggtggtct ttggggtgcc
     2641 tgcgaaagtg cgcggcgagc agacaaagcg cccgggcaag gaaaattcat gagcgtagct
     2701 gaccctgcga gtgtggaaac ggaaaagcac acaccggcgt ttagcaagtt gttgcccatg
     2761 gttgttccgg aaggactgcc gggcgaggag tttctggaag aagtgcgcgg gattttagcg
     2821 agcaagcaac tcaccaacgg cgcgcatgtt agaaaatttg aggaagctgc ggcggaatac
     2881 ttaggggttg cgcattgcgt agcggtttcg agttgcacgg cgggactgct cctggttttg
     2941 aaagctctgg atttgcgcgg ggaagtaatt ctgccaagtt ttacgtttca cgctacggcg
     3001 cacgcggtgg cgtggaatgg tttaaccccg gtgtttgcgg attgcgatgc gaaaacattt
     3061 tgtgtggcgt ctaaagcggt gcgggcgcaa atctcaagcc gaacggcggc gattctagta
     3121 gtgcatttgt tcgggaatcc cgcggcggta agggaattgg aagaaatcgc ggtggaattg
     3181 caggtgccgc tgatttttga tgcggcgcat gcgttaggga gcagcactgg tggaaggcgc
     3241 atcgggagat tcggaacggc ggaagtgttc agtttttcgc ctacaaaact ggtggtggcc
     3301 ggcgaaggtg gattgattac gacgcgggat gcggggctag ccgaaaaatt ccgagcggcg
     3361 cggaattacg gagacgcagg caactacgat ccggaaattc tgggagtgaa tgcgcgcatg
     3421 actgaaataa atgcagcgat ggcgctgcat ggtttggcgg gattggaagc ccggatagag
     3481 cggaggaatg agattcgaac gcggtacgag cggaaccttg aggatattgc ggggctgcga
     3541 tttcaggaga tttcggaagg cgggcgatcc acgtgcaaag attgttcagt gattgcggac
     3601 caaagagaat tcgggcattc gcgcgattgg ctggtggaat tgctgcatcg cgaaaatatt
     3661 caagcgcgaa aatatttttg gcccccagtg caccggcaga aactttatag cgcgatctgg
     3721 gacggccggc cattgccggt gacggatatg atttcggatg gcgtggtgag tctgccgatt
     3781 tattcttcgc tgagggatga aagcgttgac aaagtgtgcg aagttattcg gcgggcgcac
     3841 cgaacggaaa attccaaatg ggccacgggg gctgcctaat cgtgaaaacg aaaccgagcg
     3901 tggatggcga agcgaatcgt cgcgttctag tgttgggtgg cgcgggatat ctgggatcgg
     3961 tgttggtgcg gcagttactt gagaagggcc ggcgcgtgcg ggtgctggat tcgttcatgt
     4021 tcggtgagca ggcgctcgat ggagtgagat gccatccgaa tttggaggtg gtacgcgggg
     4081 acgtgcgcga tgtgggcatg gtggtgcggt gcatgcgcaa ttgcgacgcg gtgattcacc
     4141 ttgccggaat tgttggtgac ccggcgtgcg aagaaaataa agagttggcg gtggaggtga
     4201 accgtgcggc gacgcgcatg cttgcgaacg tggcacggga atgtggggtg cggcgattta
     4261 tttttgcgtc gtcgtgcagc gtttatggt
//
LOCUS       DATCBB010000575         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_117339, whole genome shotgun sequence.
ACCESSION   DATCBB010000575 DATCBB010000000
VERSION     DATCBB010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117339"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VII37_06310"
     CDS             complement(<1..832)
                     /locus_tag="VII37_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5069378.1"
                     /translation="MSTATDKAKRRDGKKPEVKKPEAKNAEMKKPETKNSNGMKASKS
                     DVERSLRMYRRMLMIRLFEEQVNELYTRALMPGLAHLYVGEEAVAVGICEALRDDDYI
                     TSTHRGHGHCVAKGAAPNRMFAELLGKEAGYCKGKGGSMDIADPATGNLGANAIVAGS
                     AGIATGAAFSAKHKGKGQVAVCFFGEGALGQGVVYEVMNLAALWKLPVIYVCENNMYN
                     EYTHFLETTAGDILSRGPAFGVPSESVDGQDVNAVYEVASRLVKRGRDGEGPSFLMCN
                     T"
     gene            complement(829..1710)
                     /locus_tag="VII37_06315"
     CDS             complement(829..1710)
                     /locus_tag="VII37_06315"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5069379.1"
                     /translation="MANLGFIGLGVMGSELVNRLLSKGHSVVGYNRTRSKAEWLVKKG
                     MKWGDSPRAVAASTDVIFSMVTNSAALAAITEGPDGILAGLTPGKVYADISTVSPAFS
                     REVAAKVRAKGCDMVDCPVSGSVITLQEGKLSVMVGGRSETFERIKPLLLDIGPKVTH
                     VGDNGLALVIKISANLSLAVQMLAFSEGVLLAEKSGIKREVAVDVLVNSAVASPMIKY
                     RGPFVLQLPPEAWFNVNMMQKDMLLALELGRQVDVPMPTAAISNEFLTDARAMGLVEQ
                     DFAVVFDVLAHMSGVKR"
     gene            complement(1743..2468)
                     /locus_tag="VII37_06320"
     CDS             complement(1743..2468)
                     /locus_tag="VII37_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY5069380.1"
                     /translation="MYKIVQTSRLYEQIVQQIEASINSGQLKLGDQLPAERELAQQFG
                     VSRTAVREAVKTLREKGLVEAHPGRGTFITNGSSHSIWQSLDRMIRDGQPEAMVHLVE
                     VREILEPEIASLAAARAEKDDIAAMREAVEVMDNSKTEPEAFIEADLDFHLALAEAAS
                     NPLILSLIDSIVGVLREQRMGIFAVEGGAERGQYHHKKILEAVEHRDSNGARASMRAH
                     LRQVREDSGRSPHGGSNFTKLNL"
BASE COUNT          611 a          891 c          675 g          670 t
ORIGIN      
        1 aggtattgca catcaaaaag gaagggcctt cgccgtcacg cccccgcttc accaagcggc
       61 tggccacttc gtagaccgcg ttcacatctt gcccgtctac gctctccgaa ggcactccga
      121 acgccggccc acggctcaaa atgtcgcccg cggtagtctc caggaaatgc gtgtattcgt
      181 tgtacatatt attttcgcag acataaataa cgggcagctt ccacagcgcg gccaggttca
      241 tcacttcata gaccacgccc tggcccagcg ctccttcgcc gaagaagcac actgcaactt
      301 gtcctttacc tttgtgcttc gcggaaaatg ccgcgcccgt cgcgattccc gcgcttcccg
      361 caacaatcgc attcgcgccc aggttgccgg tcgccgggtc ggcaatgtcc atcgagccgc
      421 cctttccttt gcaataaccc gcttccttgc ccagcaattc ggcgaacatt cggtttggcg
      481 ccgcgccctt tgccacgcag tgcccatgtc cgcgatgcgt gctggtaatg taatcgtcgt
      541 cacgcaatgc ttcgcaaatt cccaccgcca cggcttcttc gccgacgtac aaatgcgcga
      601 gaccgggcat taatgcgcgt gtgtacagct cgttcacctg ttcttcgaac aaccggatca
      661 tcagcatccg tctatacatg cgtagggagc gttccacgtc ggacttcgac gctttcatcc
      721 cgtttgaatt tttcgtttcg ggctttttca tttcagcgtt ttttgcttcg ggctttttca
      781 cttcgggctt cttgccatcg cgcctcttcg ccttatcggt ggcagtgctc atcgcttcac
      841 tcccgacatg tgcgcgagaa catcgaacac cactgcgaaa tcctgctcga ctaatcccat
      901 cgctctcgca tccgtcaaaa attcattcga aatcgctgca gtgggcatcg gcacatcaac
      961 ctggcgtccc aactccagcg ccagcagcat gtccttctgc atcatgttga cgttgaacca
     1021 tgcttccggc ggcaattgca ggacaaatgg tccacgatat ttaatcattg gcgaagcaac
     1081 tgcgctgttg accagcacgt ccaccgctac ttcacgcttg attccactct tctcggcgag
     1141 caacacgcct tctgagaaag ccagcatctg caccgcaagg cttaagttcg ctgatatttt
     1201 gatcaccagc gctaagccgt tgtcccccac gtgggtaact ttcggtccga tatccagcaa
     1261 caacggctta atacgttcga acgtttccga gcgtccgccc accatgaccg aaagcttccc
     1321 ttcttgcagc gtgatcacac taccggaaac cgggcagtcc accatgtcgc aacctttagc
     1381 gcgaactttg gctgctactt cacggctgaa tgccggactc accgtgctga tgtcggcata
     1441 aacttttcct ggagtaagac cggcaagaat tccgtcgggg ccttcggtta tggctgccaa
     1501 cgcggcggaa ttcgtgacca tcgaaaaaat gacatcggtc gatgccgcaa cggctcgcgg
     1561 cgaatcgccc cacttcatgc ccttcttaac cagccactcc gcttttgagc gcgtgcgatt
     1621 gtagccaact accgaatgac ccttgcttag caggcggttg actaactcgc tgcccattac
     1681 gcctaaaccg atgaatccca gatttgccat cagttttccg atctcctggt tacaaggctc
     1741 ttttacaaat ttagtttggt gaagttcgaa ccgccgtgcg gcgagcgacc tgagtcttcg
     1801 cgcacctgcc gaagatgcgc acgcattgac gcacgtgcgc catttgaatc gcgatgctcc
     1861 acagcttcta gaattttttt gtggtgatac tggccacgct cagcgccacc ttcaaccgca
     1921 aagataccca tgcgctgttc gcgcagcact cctacgatcg agtcaatcag cgacagaatc
     1981 agcggattgg aagcggcctc cgcgagcgcc agatgaaaat ccagatcggc ttcgatgaac
     2041 gcttctggtt ccgtcttgct gttgtccatc acttccacag cttcacgcat cgcggcaatg
     2101 tcatcttttt ccgcacgggc tgccgccagc gaggcgattt ccggctccag aatttcgcgc
     2161 acttccacca ggtgcaccat cgcttcaggt tggccatccc ggatcatgcg atctagcgac
     2221 tgccagatgg agtgcgagct accgttcgtg ataaaagtcc cgcgaccggg atgagcctcc
     2281 accaagccct tttcacgtaa cgttttaacc gcttcacgca ctgccgtcct actcacccca
     2341 aattgctggg ccaactcccg ttcggcgggt agttggtcgc ccagcttgag ctgaccgctg
     2401 ttgatggacg cttcaatctg ctggacgatt tgttcataaa ggcgggaagt ttggacgatt
     2461 ttatacatgg caaatctcca acaaaggtct cctcagtacg gacccggtat taaggtccta
     2521 ccaccgaaaa agtctagtcg ccgatcaaga gcgttcctgg ccactggttt tcattgctca
     2581 aacgtcgccg gcaacgcaaa gcgccgcaag cgcggacgtc cgtcgcgttg cgcactggcg
     2641 gtgtgacaag ttgttgacca tcgacttttc gatactgggg gacacttgta cctcgacgca
     2701 caaggtccga ccactatacc ccaagagaca catcgggaaa agaagcaact aaaactttca
     2761 tacaaagcat taaagttctt gacccgtccc ccgaaaaagt ggtacagcgg tcagacctat
     2821 tggccaccta aggagtaccg atgaaaa
//
LOCUS       DATCBB010000576         1742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_278583, whole genome shotgun sequence.
ACCESSION   DATCBB010000576 DATCBB010000000
VERSION     DATCBB010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278583"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VII37_06325"
     CDS             complement(<1..902)
                     /locus_tag="VII37_06325"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069381.1"
                     /translation="MPTQTLTTEVRMIKGVGPQRAALLAKRGILTLEDLLNYLPFRYE
                     DRIHFSNIKDIQPNGIYTIRVQVMSGQAVRTMRGRDAVFHMLVRDASGQLPVKFFHGG
                     YLEGRLKPGQSLILHGKAEIDKLRPARIEMVNPQMEMTGEQMDSTEVGRIVPIYEAIG
                     TFGSRAIRRAIYAALDLLDPHLPDVLPAALRARLSYPSRREALLHTHFPPPGESLDAL
                     NTFRSPAQQRLIFEELFLYQLSIALDRRATRKETAIAFRLREDNIREALKRILPFKPT
                     AAQKRVLGEIAADLEKPAPMNRLL"
     gene            1278..>1742
                     /locus_tag="VII37_06330"
     CDS             1278..>1742
                     /locus_tag="VII37_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069382.1"
                     /translation="MANDRLTAANKLALMLWVVAGIAGALFAYKYYFRAFPEAAVNFK
                     VSRGEALTRAKQFLSSMGEDAGSYQSAIVFEVDDNAKTYLERETGLEQANRLMSSELN
                     IFYWDVRFFKPQQEEEFHVRISPAGQVVGYEHHVAESRAGATLERAAAQLTAE"
BASE COUNT          341 a          374 c          658 g          369 t
ORIGIN      
        1 tgcagcaggc ggttcatggg cgcgggcttt tcgaggtcgg cggcaatttc gccgaggacg
       61 cgtttctgcg cggcggtggg cttgaagggc aggatgcgct tgagagcttc gcggatattg
      121 tcttcgcgca ggcgaaaggc gatggcggtt tccttgcggg tggcacggcg atcgagcgcg
      181 atgctgagct gatacagaaa taattcttcg aagatgaggc gctgctgcgc gggagaacgg
      241 aaagtgttga gcgcgtcgag cgattccccc ggcgggggga aatgggtgtg gagcagcgct
      301 tcgcggcgcg agggatagga gaggcgcgcg cgtaacgcgg cgggaagaac gtcgggcaag
      361 tgaggatcga gcaaatcgag cgcggcgtaa attgcgcggc gaatggcgcg ggatccgaac
      421 gtgccgatgg cttcgtagat ggggacgatg cggccgactt cggtggagtc catttgctcg
      481 ccggtcattt ccatctgggg attgaccatt tcgatgcgag cggggcggag cttatcgatt
      541 tcggctttgc cgtgaaggat gagggattgg ccgggtttga ggcggccttc gagatagccg
      601 ccgtgaaaaa atttgacggg gagctggccg ctggcgtcgc gaaccagcat gtgaaaaacg
      661 gcgtcgcggc cgcgcatggt gcggacggcc tggccgctca tgacctggac gcggatggtg
      721 tagatgccgt tgggctggat gtcttttatg ttggagaaat ggatgcggtc ttcgtagcgg
      781 aagggcaaat aattcaggag gtcttcgagg gtgaggatgc cgcgcttggc gagcagcgcg
      841 gcacgctggg ggccaacgcc cttgatcatg cggacttcgg tggtgagagt ttgcgtgggc
      901 acgtgtggaa gtgctgcttg attgcgtaag tacggtaatt tacttctctc tttataccat
      961 ttggggaaag cggacgcagg taaggcactg gtgcggcgaa acttgcgtgg atttggcgcg
     1021 tatcctagag taagcacgcc gcgcggcgtt ggcggacgtg gcgcgtcaat gttagaaggg
     1081 acgtgcgttg tcgggtctgt ggtgcggcag caaacagcag attcctcacg cgccgctatc
     1141 gcggcggttc ggaatgactc gtggttttga tagttgccaa gattagataa gttgcgggtt
     1201 cgggcagttg attgaaacgg ttgtcaagat atagctggaa ccaacgttag tgcggagatg
     1261 ttgggagtgg aggaaatgtg gcgaatgatc ggctgacggc agcgaacaag ctggcgctga
     1321 tgttgtgggt ggtggcggga attgccgggg cgctgttcgc ttacaaatat tattttcggg
     1381 cgtttccgga agcggcggtg aatttcaagg tgtcgcgcgg ggaggcgttg acgcgcgcga
     1441 agcagtttct atcgagcatg ggagaagacg cggggagcta tcagtcagcg attgtttttg
     1501 aggtggacga caacgcgaag acttatctgg agcgcgaaac cggattggag caggccaacc
     1561 ggctgatgtc ttcggagttg aacatctttt actgggatgt gcgatttttc aagccgcagc
     1621 aggaagaaga atttcacgtg cggatcagtc cggcggggca ggtggtgggc tacgaacacc
     1681 acgttgcgga atcgcgagcg ggggcgacac tggagcgggc cgcggcgcaa cttacggcag
     1741 aa
//
LOCUS       DATCBB010000577         3787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_52090, whole genome shotgun sequence.
ACCESSION   DATCBB010000577 DATCBB010000000
VERSION     DATCBB010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52090"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VII37_06335"
     CDS             complement(<1..760)
                     /locus_tag="VII37_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069383.1"
                     /translation="MRKSMIVIVILLAASGAFVTAQTQRQDKPAPAAIDAEKIRAHVK
                     FLSSDLLEGRGMGQRGSDIAAEYIATQFALDGLKPAGDQGTYFQEVPMVSVTTLPDTT
                     VSLVPKSGEPQQLKNLDDFVIHNETQSEVADIDAPIVFVGYGITAPEYKWDDYKGVDL
                     KGKVALLFVNEPTSDDPKFFKGKALTYYGRWTYKYEETARHGAVATLIIHRRDLASYG
                     WEVVRNSSGKDKSYLRLDGTPKLQGASWIQLDVAK"
     gene            complement(797..1261)
                     /gene="nrdR"
                     /locus_tag="VII37_06340"
     CDS             complement(797..1261)
                     /gene="nrdR"
                     /locus_tag="VII37_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY5069384.1"
                     /translation="MKCPFCAHIDDKVIDSREGRAGDTIRRRRECLKCSRRFTTYERI
                     DEIPYMVAKKDGRRERFERQKILQGLLKACEKRPVATPKLEAIVDEIEGVVHEATERE
                     LTTSEIGEMIMRGLKKLDKVAYVRFASVYMDFKDVQEFMAELKGLLKERTKK"
     gene            complement(1379..2419)
                     /locus_tag="VII37_06345"
     CDS             complement(1379..2419)
                     /locus_tag="VII37_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5069385.1"
                     /translation="MLGLLALGIIAYQASTFLHLATFSGSKLLHSVRDADPYYIALAM
                     LAIYSCYGIRSLRWQVFQRNIGSTNFWQIYKLTLAGFAAVFLLGRAGEPIRPLLLAKK
                     AHLPIADTFGIYVLERIFDMASVAVISGVGLILFGMRPHAGDMAAQLETAAKTTGAIL
                     FAGVLAATIALVYLRVHGTALLERRLQATIHAGGWRSSLARVVLGFVRGVQTIRTWKD
                     LILATVYSALHWLVVALVYFWVSHSFTGTLHTLGVADSLIVLTFTLVGSAIQLPGIGG
                     GSQAGAIIAYTTIFGVEREPAVAAAMVLWAITFAGCSILGVPMLIHEGWGLRELKRLA
                     AEEKKEVAAALE"
     gene            2580..2936
                     /locus_tag="VII37_06350"
     CDS             2580..2936
                     /locus_tag="VII37_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5069386.1"
                     /translation="MTETILKRFENPDEVRVFNKGRFEIIHIGGLTIGRASYEPGWKW
                     SQDVAPLAGTALCEVEHVGLVVSGRAIAAFADGTQTELTPGNLFYIPPVAHDSWVVGN
                     EPYVSLHFMGAAHYAK"
     gene            3405..3536
                     /locus_tag="VII37_06355"
     CDS             3405..3536
                     /locus_tag="VII37_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069387.1"
                     /translation="MSREKIESLMHAMNQSHVELSISEADGEAGPTGACAGQGEDCP"
BASE COUNT          837 a          951 c         1073 g          926 t
ORIGIN      
        1 ttttggcgac gtctagttga atccaggacg cgccttgcaa tttcggggtg ccgtccaaac
       61 gcagatagga tttgtctttg ccgctcgaat tgcggacaac ttcccagccg tagctggcca
      121 agtcgcggcg atgaataatc agcgtggcaa ctgcgccgtg gcgcgccgtt tcttcatatt
      181 tgtaggtcca cctgccgtag taggtgagcg ccttgccttt gaaaaacttg ggatcgtcgg
      241 aggtgggctc attgacgaac agcagcgcta cttttccttt tagatccacc cctttgtaat
      301 cgtcccattt atattcgggg gcggtgattc cataacccac gaacacgatg ggggcatcga
      361 tgtccgcgac ttccgattgc gtttcgttat gaattacaaa atcatcgaga ttttttagtt
      421 gttgcggctc accgctcttg ggtacaagcg aaacagttgt gtctggcaga gtggtgacgc
      481 tgaccatggg cacttcctgg aagtaggtgc cttgatcgcc ggctggtttg aggccgtcga
      541 gtgcgaattg cgtggcaatg tattcggcgg cgatgtcgct gccgcgctgg cccatgccgc
      601 ggccttctag aagatcgctc gagagaaact taacgtgggc gcgaattttt tccgcatcga
      661 ttgctgctgg agctggttta tcttgccgct gcgtctgtgc ggtaacaaaa gcgccgcttg
      721 ccgccagtaa aatcacaatt acgatcatcg atttgcgcat gggtcctcgt gggtgcgtaa
      781 ttctggtgca ctgccattac tttttagtgc gctctttcag caagcccttc aactccgcca
      841 tgaattcctg cacatcttta aaatccatat acacggacgc gaagcgcacg taagccactt
      901 tgtcgagttt cttgagtccg cgcatgatca tttcgccgat ttccgaggtg gtcagctcgc
      961 gctcggtggc ctcgtgtacc acaccttcga tttcatccac gatggcttca agttttggcg
     1021 tagctacggg gcgcttctcg caggccttta gcagaccctg caaaattttt tgacgttcga
     1081 agcgctctct gcgcccgtct ttcttggcga ccatgtaggg aatttcgtcg atgcgttcat
     1141 aagtggtgaa tctgcgggag catttgaggc attcgcggcg gcggcgaatg gtgtcgccag
     1201 cgcggccttc gcgagaatcg atcaccttgt cgtcaatgtg ggcacagaag gggcacttca
     1261 ctcgggataa tctcctgtca gctgcggagc ggacaaaacc gaattgatat tttgagtttg
     1321 caagaattct ggccgcctat tgtagcgagt ttatttcaag ttgctcacat cagggtgctc
     1381 attctaacgc ggcggccact tctttttttt cttccgcggc caatcgtttc agctcgcgca
     1441 agccccagcc ttcgtgaatc agcatgggga cgcctagtat gctgcacccc gcgaaggtta
     1501 tggcccacaa caccatagca gcagcgaccg cgggttctct ttctactcca aaaatcgtgg
     1561 tgtacgcaat gatggcgccc gcctgcgatc ctccgccaat gcccggcaac tgaatggccg
     1621 agcctaccag agtaaatgtc aggactatca aagagtcggc cacgccgagg gtgtggagcg
     1681 ttcctgtgaa actgtgcgag acccaaaaat aaacgagcgc gactactaac cagtgcagcg
     1741 cagaataaac cgtggcgaga atcaagtctt tccaggtgcg gatggtttgc acaccgcgga
     1801 cgaaacccag aacgacgcgc gccaaggaac tgcgccatcc accggcgtga attgtggctt
     1861 gcaggcggcg ctcgagaagt gcggtaccgt ggacgcgcag atagaccagc gcgatggtgg
     1921 cggccagtac gcccgcgaac aaaattgcgc cggtggtttt ggcggcggtt tccagttgag
     1981 cggccatatc accggcatgc ggtcgcatgc cgaacaagat taaacccacg ccggaaatga
     2041 ccgcgacgct ggccatatcg aaaatacgct cgagaacgta aatgccgaag gtgtcggcaa
     2101 tgggcaggtg cgcctttttt gccagcagca aaggcctgat tggttcacct gcccggccca
     2161 ggagaaaaac ggcggcaaat ccggccagcg tgagcttata aatttgccaa aaatttgtgc
     2221 tgccgatgtt gcgctggaaa acttgccagc ggagcgagcg gattccatag caagagtaaa
     2281 ttgccagcat ggccaaagcg atgtaataag gatcggcatc gcgcacagag tgaagcaatt
     2341 tggatccgct gaaagtcgcg aggtgcagga aggtgctcgc ctggtaggcg atgatgccca
     2401 aggcaagcaa tccaagcacg accaatatgg ttttcctgga tggcgaacgc atgtgacatg
     2461 agcctactga gcacatgtat gctcgtcaag ttttagagag ttcagtgaag gaatgctgcg
     2521 aaactgcaaa atttcagtag tggccgttcg aattaccgcc gtgcagacga ggagagacca
     2581 tgacggaaac tattttgaag cgattcgaaa atccggacga ggtgcgcgtt ttcaacaaag
     2641 ggcgatttga gattattcat atcggtggat tgaccattgg acgcgccagc tacgaacccg
     2701 gatggaagtg gtcgcaggat gttgctcctc tggctggtac agcattgtgt gaagtggagc
     2761 atgtcgggct ggtcgtttca gggcgagcga ttgccgcttt cgctgatgga acgcagacag
     2821 aattgacgcc gggaaatttg ttttacattc cgccggtggc acacgacagc tgggtggtcg
     2881 gaaatgagcc ttacgtgtcg ctccatttta tgggcgcggc gcattatgca aagtaggcgc
     2941 aggctgcaga ctattcctga taggaagcgc ggttcggcgt aacatgtgct tatgaagaag
     3001 gctgtcacca gtgcacataa tttattgatt ttcttatgcg tggcggctgc tttgttggta
     3061 gcgctcgcgc cttctgttgc gctcgctcta aaattacttg ttcctgagtg gttttttgtt
     3121 gcgctggtcg tcattgcgct agtcccgacg caaaaagaca cctggattcc acagttagcg
     3181 ccgacgcttt ctgttttctc tccacgacct cccccgattc agtagttaac cgcatcagca
     3241 aatcaattgt tacgaaattg aaatctcctt gcgattcgtc gcgccgcggc gatgacacgt
     3301 aaacccgtca tgcagtcgtt tgagggaaaa tgtcattcaa attgctcatt cgcaggtttg
     3361 ttatggcttt cgtgttggga ggatattcgg tacttggagt tggaatgagt cgagagaaaa
     3421 tcgaatcgtt gatgcacgcg atgaaccaat cgcacgtcga attatcaatt tccgaggctg
     3481 acggtgaggc gggtccgaca ggagcttgcg ctggtcaagg cgaagactgt ccgtgaacca
     3541 cgtattcaaa ctttttggct cccggcttcg tattgttctg ggtggctcaa tgtttgacaa
     3601 tgcaagccca cgttcaaact acgaacgtcg ggtaccctcg gtctgactgt gtacttggat
     3661 acatgaacta ggtccgggct ctcatcaaga gttgaaaggc ttacatgcga ctggtggtgg
     3721 aaaatgtcag caaagaatat cgcggcggcg cgcaaggcgt ccgtgcgctg agtaacgttg
     3781 ggttttc
//
LOCUS       DATCBB010000578         1792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_213900, whole genome shotgun sequence.
ACCESSION   DATCBB010000578 DATCBB010000000
VERSION     DATCBB010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213900"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="guaB"
                     /locus_tag="VII37_06360"
     CDS             complement(<1..1126)
                     /gene="guaB"
                     /locus_tag="VII37_06360"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY5069388.1"
                     /translation="MKDGPIAEGLTFDDVLLLPGKSSVLPTEVDTQTNFTRKLALNIP
                     LASAAMDTVTESRLAIAIARQGGIGMVHRNMTIERESEEVDRVKRSESGMIVDPVTIS
                     PDITLRQALEIMTKYRISGLPVTRGARLTGILTNRDLRFEKNHSQLVSEVMTKDNLVT
                     VPVGTTLDEAERILQKHRIEKLLVVDQDYNLKGLITVKDIQKKLAYPRAAKDEQGRLR
                     VGAAVGATGDFLERAVEMARAKADVLAIDTAHGHSARVMEAIKAIRNRLPEMQLVAGN
                     VATFEGAKDLIVLGIDGLKVGIGPGSICTTRVVTGAGVPQITAIMEASRAAQGTDVPV
                     IADGGIKFSGDISKAIAAGASFVMIGGLFAGTEEAPGETIL"
     gene            1453..>1792
                     /locus_tag="VII37_06365"
     CDS             1453..>1792
                     /locus_tag="VII37_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5069389.1"
                     /translation="MTIAPVFWRRNFSLPPPEDRQFQVEPESRLLAHCHWQPDKRSDA
                     PVIVIVHGLEGSSNSDYARGIAEAAFKRGYNAIRINQRNCGGSESLTPTLYNSGMSSD
                     YRAVCGELTDE"
BASE COUNT          380 a          552 c          453 g          407 t
ORIGIN      
        1 acaaaatcgt ttcgcccggg gcctcttccg tgccggcaaa caacccgcca atcatcacga
       61 acgaagcgcc ggccgcgatc gctttactga tgtcgccgga aaatttgatg ccgccatccg
      121 caatcaccgg cacgtcggtt ccttgcgccg cgcgcgacgc ttccatgatg gcagtgatct
      181 gcggcacacc agcgcccgtc actacccgcg tggtgcaaat cgatcccggg ccaatcccga
      241 ccttcaatcc atcaatccct agcacaatca aatccttagc gccttcgaaa gtggctacat
      301 ttcccgcgac caactgcatt tccggaaggc gattacgaat ggccttgatc gcttccatca
      361 cccgcgcgga atgtccgtgc gctgtatcga tggccagcac gtccgccttg gcgcgcgcca
      421 tttccaccgc gcgttccagg aaatctccgg tagcgcccac cgcggctcct acacgcaatc
      481 gaccctgttc gtccttggcc gctcgcgggt aggcgagttt cttctgaata tcctttacgg
      541 tgatcaggcc tttgaggttg taatcctggt ccaccaccag cagtttttcg atgcgatgct
      601 tctgcagaat gcgttcagct tcgtcgagcg tagttcctac cggaacggtg acgaggttgt
      661 ctttggtcat cacctcgctc accaattgcg agtgattctt ttcaaagcgc agatcccgat
      721 tcgtaagaat ccctgtaagt cgcgcgccac gggtaactgg caagcccgaa attcggtact
      781 tggtcatgat ctccagcgcc tgccgcagcg taatatccgg cgaaatcgtc accggatcca
      841 cgatcattcc gctctctgag cgcttcacgc ggtcgacttc ctcgctctcg cgctcaatgg
      901 tcatgttgcg gtgcaccatg ccaatgccgc cctgccgcgc aatggcaatc gccagtcgtg
      961 attccgttac ggtatccatg gcagcggacg ccagcggaat attcagcgca agtttgcgtg
     1021 tgaagttggt ctgcgtatcc acttccgttg gcagcaccga ggactttccg ggcagcagca
     1081 acacgtcatc gaaagtaaga ccctcggcta tcggtccatc cttcattcac aagccccgca
     1141 gaggaaattt taagaacaaa agtcagcgtg tcgcgaaaac tgagcgctaa aacaacgcgg
     1201 cccagcaggc ttctgctggg cttcaaactg attcatcgaa tcgaagaaat tgttcaccgt
     1261 ggatatttca ttgtaggggc acgcatggtc ggggtcaagt tgggcggctt atcgtagcgt
     1321 gtgcgtgcct gctcaaaaaa atcccgtcat cacgcatgtt actgtaatta acgaatattg
     1381 cggggctatc cacgtatgcc gctgtttctc gaaaatttcg agccccaccc actgcttcgc
     1441 ggcgcgcata tcatgaccat tgctcctgtg ttttggcgaa gaaacttttc tctgccaccg
     1501 cccgaggatc ggcaattcca ggtcgagcct gaatcccgcc tactggcgca ctgccattgg
     1561 caaccggaca aacgttcgga cgcaccggtg atcgtcatcg tccacggcct ggaaggctca
     1621 agtaattccg actacgctcg cggcatcgcc gaagctgcat tcaagcgcgg ctacaatgcc
     1681 attcgcatta atcaacgcaa ttgtggcggc agtgaatcac tgacacctac gctttacaat
     1741 tccggaatga gctcggatta tcgcgccgtt tgcggggagc taaccgatga ag
//
LOCUS       DATCBB010000579         1996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_409189, whole genome shotgun sequence.
ACCESSION   DATCBB010000579 DATCBB010000000
VERSION     DATCBB010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409189"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VII37_06370"
     CDS             complement(<1..942)
                     /locus_tag="VII37_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY5069390.1"
                     /translation="MAHIVPHPLALVGGLALAIIILVDAFEAIILPRRVTRKIRVTRL
                     FYRTTWSFWKAVVNKLPARKTREALLGGYGPCSLLLLIGIWALGLVLSFGTMQYGAGS
                     AIEIHEAAPPGFAMDVYLSATTFFTLGMGDVVPRVPLARFLLVAEAGLGYGFLAVIVG
                     YLPFIYGSFSRREVNISLLDARAGTPPTAGELLRRYSYPEGQEALRDLLAEWELWCAE
                     LMESHLSYPVLAYFRSQHDNQSWIASLTAILDTCALVKAGIEGACERQADLTFAIARH
                     AVVDLSQVFGTAPRAAATDRLSNADLRLIRDTLAAHGM"
     gene            complement(1001..1426)
                     /locus_tag="VII37_06375"
     CDS             complement(1001..1426)
                     /locus_tag="VII37_06375"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5069391.1"
                     /translation="MDRKASAVWTGGLKDGKGTLSTDSGVLKQAQYSFSTRFENGIGT
                     NPEELLAAAHAGCFTMALSGQLGKAGMTAQKLETTATVTLAKDGEGFSITKSHLDLVA
                     NIPGADKAKFDAAVKAAETGCPVSKLFKAEITVNARLQS"
     gene            1534..>1996
                     /locus_tag="VII37_06380"
     CDS             1534..>1996
                     /locus_tag="VII37_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEY5069392.1"
                     /translation="MIKILQHTAHRPWPLLAGPWIMKQEWHDLLFAHWAVPADVLCPL
                     IPRALEIDTFDGSAWLGVVPFRMSGVRMRGMLPMPGLARFPELNVRTYVVRDGKPGVW
                     FFSLDAANALAVWGARRAFHLPYFWAAMRCEEDSGWICYESNRKHSGVPPAS"
BASE COUNT          419 a          512 c          633 g          432 t
ORIGIN      
        1 catgccatgc gctgcaagcg tgtcgcgaat caagcgcaga tcggcgttgc tgaggcgatc
       61 ggtggccgcg gcgcgagggg cggtaccaaa aacctgggac agatccacta ccgcgtgacg
      121 cgcaattgcg aaagttagat cagcctgacg ttcgcaggcc ccttcgatcc cggctttaac
      181 caacgcgcag gtgtccagga tggcggttaa cgaagcgatc caggactgat tgtcatgttg
      241 cgaacggaag tacgcgagca ccgggtagga aagatgactc tccattaatt ccgcgcacca
      301 caattcccac tccgcaagta gatcgcgtaa agcttcttgg ccttccgggt agctgtaccg
      361 tcgcagaagt tctccggcgg taggcggcgt tcctgcacgg gcgtcgagga gcgagatgtt
      421 cacttcacgg cgagaaaaag aaccgtagat aaatggaaga tatccgacga tcacggcgag
      481 aaatccgtag cctaatccgg cttcggcgac gagtaggaag cgcgctaatg gaacgcgcgg
      541 aacaacatcg cccatgccca aagtgaaaaa agtggtagcg ctgagataca cgtccatggc
      601 gaaacctggt ggggctgcct cgtgaatctc aatcgcggag ccggcgccgt attgcatggt
      661 cccaaaactc agcaccaggc cgagggccca gatgccgatg agcagtaaca gggaacacgg
      721 accatagcca cccagcagcg cttcccgcgt tttgcgagcg gggagtttgt tcaccacggc
      781 tttccaaaac gaccaggtcg ttctgtaaaa gagccgcgtc acacgaattt tccgcgtaac
      841 gcggcgcgga agaatgatgg cttcaaaggc gtcaaccaga ataatgatgg ctagcgccaa
      901 gccgccgaca agcgcgagtg gatggggaac gatgtgcgcc attatttaga ctttcgtgat
      961 ttgaatgcgc gagatcggca tgacgaaaag cgctggcagg tcaggattgc aggcgcgcgt
     1021 tcacggtgat ttcggctttg aacagttttg aaactgggca gccagtttcg gcggctttga
     1081 cggcggcatc aaatttggct ttgtcggctc cagggatgtt ggcgacgagg tcgagatggc
     1141 ttttggtgat ggaaaaacct tcgccgtctt tggccagcgt aacggtggcg gtggtttcga
     1201 gtttctgcgc ggtcatgccg gctttgccga gttggccgga gagcgccatg gtgaaacatc
     1261 cggcgtgagc ggcagccaac aattcctcgg gattggtgcc gatgccattt tcaaaacgag
     1321 tgctgaagga atattgcgct tgcttcagta cgccgctgtc ggtcgacaga gtgcctttgc
     1381 cgtccttgag cccgccagtc cacactgctg atgctttgcg atccatgtgt gcctcttttc
     1441 tttgcgctgt cgcgtagaca ttcgattctg cgcgattgag gaagggtata ttgggcgcag
     1501 caagatgtcg agaagcgggt ttgttgggtt ctgatgatta aaattttgca gcacaccgct
     1561 caccggcctt ggccgttgct agccggtccg tggatcatga agcaggagtg gcacgatttg
     1621 ttattcgcgc attgggccgt gccggcggat gtattgtgtc cgctcatccc acgagcgctg
     1681 gagatagaca cgtttgacgg atcggcgtgg ctgggcgtgg tgccttttcg catgtcgggt
     1741 gtgcggatgc gaggcatgct gccgatgccg ggcctggcgc gtttcccaga attaaacgtg
     1801 cgcacttatg ttgtacgaga tggcaaaccg ggtgtgtggt tctttagcct ggacgctgcg
     1861 aacgcgctcg cggtctgggg cgctcgcagg gcatttcatc tgccgtattt ttgggcagcg
     1921 atgcgctgcg aagaagactc gggctggatt tgttacgaaa gtaaccggaa gcacagcggg
     1981 gtgccgccgg catctc
//
LOCUS       DATCBB010000580         1579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_149446, whole genome shotgun sequence.
ACCESSION   DATCBB010000580 DATCBB010000000
VERSION     DATCBB010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149446"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1296)
                     /locus_tag="VII37_06385"
     CDS             complement(217..1296)
                     /locus_tag="VII37_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY5069393.1"
                     /translation="MRGNDEQQEEMFSYVSMEKRVPKDHPLRRVRAMTDTALRGMSPD
                     FDAMYARVGRPSIAPERLLRALLLQALYTVRSERQLMERLEYDLLFRWFVGLSMDEKV
                     WDATVYTKNRERLLEGEIAEVFFEQVLGQAKEQGLLSDEHFTVDGTMIEAWANRRSFQ
                     EKKDPPEQGSGRGGEKLLRDTHESKSDPEARLYKKSAAGEARPSYLGHVVMENRNGLV
                     MKPCVTEAGTRQERDAALRMLAELLKEIRETRKAGAITVGADKGYQEQDFIEGLRKLS
                     AIPHVAEYEKRRRSWLTASERAGLGFQISQSKRKLVEKIFGWMKVVAGQRKTKFRGRR
                     RVHWMFSLGAAAFNLIRMAKLIPAA"
BASE COUNT          283 a          564 c          337 g          395 t
ORIGIN      
        1 tggttaagca agcaatcgtt cccaccttgg cgccgccagc cagtagcgta ggcttcacag
       61 gttgcggaaa aactcacgga atttcatccg cagggccttg cctaactaat tttagctcct
      121 acagcatgtg gggcagcgat ggttggtcgg ccgcttccac ctactcggag cgctttttct
      181 gttcttgccg cgtacttccg ttcgatccgt tttgctttac gctgccggga tcaacttcgc
      241 cattcggatc aaattgaagg ccgccgcacc cagcgaaaac atccaatgca ctcgccgtcg
      301 ccctcgaaat ttggtcttcc tctgtcccgc cacaactttc atccagccaa agattttttc
      361 caccagtttc ctcttgctct gactgatctg aaatccaagg cccgctcgct cgcttgccgt
      421 caaccaactc cgtcgtctct tttcgtactc cgccacgtgt ggtatcgccg atagtttccg
      481 caatccctca atgaaatcct gctcctgata tcccttgtct gctcccaccg tgatcgctcc
      541 cgctttgcgc gtctcccgga tttctttcag caactcggcc agcatccgca acgccgcgtc
      601 ccgctcttgc cgtgttcccg cttccgtcac gcacggtttc atcaccaacc cgttgcgatt
      661 ttccatcacc acgtgtccca aataactcgg cctggcttct cccgccgcac tcttcttata
      721 caaccgcgct tccggatcgc tcttgctctc gtgcgtgtcc cgcaataatt tctctcctcc
      781 gcgtccgctg ccttgctccg gaggatcctt cttctcctga aaactccgcc gattggccca
      841 cgcctcgatc atcgttccgt ccaccgtgaa gtgttcgtcc gacagcaacc cctgttcctt
      901 ggcctgtccc agcacttgct cgaaaaatac ttccgcgatt tccccctcca gcagccgctc
      961 ccggttcttc gtgtataccg tcgcgtccca caccttctcg tccatgctca gtcccacaaa
     1021 ccagcggaac agcaagtcat actccagccg ctccatcagc tgccgctcgc tgcgcaccgt
     1081 gtacagcgct tgcagcagca acgcccgcaa caacctctcc ggcgcgatcg acggccgccc
     1141 cacgcgcgca tacatcgcgt caaagtccgg cgacatcccc cgcaacgccg tgtccgtcat
     1201 cgcccgcact cgccgcagcg gatgatcttt cggcacgcgc ttctccatcg aaacgtaact
     1261 gaacatctct tcctgctgct cgtcgtttcc tctcatatac acttagacgc tctcgctctt
     1321 atccggttcc ggaaaaccga ctttttccgc aacctgttca gcctgcgcgg tttgtgaatt
     1381 agcaaggcgg attgggcatc gtttgtttag cctgtgtgag agcaaatgtc gcgctgacaa
     1441 acgcgcgcag gcttaaagcc tacgctacca aaacccataa atttactttg agttagctgc
     1501 cttgcggatc agctcttcca cgcgggccac gtcttccggc acatccacgc ttacggaatc
     1561 atgcggcgtt tcgacgacg
//
LOCUS       DATCBB010000581         3724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_83847, whole genome shotgun sequence.
ACCESSION   DATCBB010000581 DATCBB010000000
VERSION     DATCBB010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83847"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..931)
                     /locus_tag="VII37_06390"
     CDS             complement(95..931)
                     /locus_tag="VII37_06390"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY5069394.1"
                     /translation="MLLTIDVGNTNTVLGVFRDTELVANWRLSTAREQTVDEYGVLTR
                     NLFTLAGLDRDAITGVMISSVVPPVNWTLSEMARIYLGKKALFVEPGVKTGMAVLVDN
                     PLEVGADRIVNGVAAFHEYGPCIVVDFGTAITFDVISARGEYLGGAIAPGIGISSEAL
                     FARAARLSRVEIKDPGKVIGTNTVSHMQSGLYYGFVDLVDGMLKRMKDELGHEVTVVA
                     TGGHAKLVARGSKHIQHTDEFLTLKGLRLIWEKNQASDASKAGRLREASAGAAEKKAP
                     RQ"
     gene            complement(959..1978)
                     /locus_tag="VII37_06395"
     CDS             complement(959..1978)
                     /locus_tag="VII37_06395"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY5069395.1"
                     /translation="MAVRVKPSKDIAGSTERQVAALLTLLAENGTIVISGARIAREIG
                     VSRSTVFRWVTRLRELGVKVKGRAATGYFLEQVPDILTADMLRQRLRGSLFGKRIYHF
                     FKTDSTNRVALELGHASEAEGAVVLAEEQTAGRGRAGHTWHSERATGIYATILLRPRL
                     APVQAPLLTMMAGLSAHAAVQGVTGLNVDLKWPNDLVINGKKVGGILTEMHAEPAQVR
                     FVIVGIGINVNQEKFPADLASKSTSLRIETGRQQSRLEVLVQFLRQFERDYRELLDEG
                     AKSVVKRFEATSSYARGKRVRVTNGRESFTGTTAGLEEEGLLRVKRDTGETTTVIAGD
                     ITEIR"
     gene            complement(2018..>3724)
                     /locus_tag="VII37_06400"
     CDS             complement(2018..>3724)
                     /locus_tag="VII37_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5069396.1"
                     /translation="QGLLEKVTEHTHAVGLCERSRTIVEPRASTQWFCKMKPLAEPAI
                     AAVERGEIRIVPDNRREEYFNWMRNIRDWTLSRQLWWGHRIPAWYCEEFGHVTVARET
                     PAKCATCGSGKLRQDPDVLDTWFSSGLWPFSTLGWPEETKDLKTYYPTTLLITGYDIL
                     FFWVARMIMLGIHFTGQVPFRAVYLHSLVRTGSGEKMSKSKGTGLDPVLLNKQYGTDA
                     MRFCLASMAAPGTDIVLSDDRLAGARNFANKIWNAARFLFVNLEKFEQGGATLQELAA
                     PEIRAKAPYALTGAVPLRDAWIFARLAVITEQMNYALANYRFHEGAQGVYQFFWGDFC
                     DWYIEWVKPDLQSDDKERAVVAWRNLFAVFEAALRLLHPFMPFLTEELWHQFPQQAGA
                     KSIALERFPEVRESWRNNAALEEFGLLQEIITAVRNIRAEMKLDPKKKMAAELSVADE
                     RTRAAVESNRDVIVRLGLLTDLKITGGSLAQSGAVRSSAKFDLRIEYAETVDAAAECA
                     KITKELEGLRKAIGSKEKQLGDETFRSRAPEKIIRGLEATLATQRIEMQKLTERFEQL
                     DC"
BASE COUNT          820 a         1293 c          772 g          839 t
ORIGIN      
        1 ttcttcgatt tccgtgaagt agttgaagta gttccagccg tccatgtgtg acgacaaaaa
       61 ggggtaaccg accagttgct gaactctgcc gcggctactg tcgcggagct tttttctctg
      121 cggcgccagc actcgcttcg cgaagccgcc ccgccttgct cgcatccgaa gcctgattct
      181 tttcccagat caaccgcaaa cccttcagcg tcagaaattc gtcagtgtgt tgaatatgtt
      241 tcgaaccccg cgctaccaat ttcgcatgcc cgccagtagc caccaccgta acttcgtggc
      301 caagctcatc cttcatgcgc ttgagcatgc catcaacgag atccacaaat ccgtaataca
      361 acccggactg catatgactc accgtatttg tgccgatgac ttttcccggg tccttaatct
      421 ccacgcgcga tagccgtgcc gctcgcgcaa acaacgcttc cgaagaaatc ccgatccccg
      481 gtgcaatcgc tccacccaga tactcaccgc gtgcggaaat tacatcaaac gtaatcgccg
      541 tcccaaaatc cactacaata cacggcccgt actcatgaaa cgccgccacg ccattcacaa
      601 ttctgtccgc gcccacttcc aaaggattat caaccagcac cgccatcccg gtcttcaccc
      661 ccggttccac gaatagcgcc tttttcccca gataaatccg cgccatttcg ctcagcgtcc
      721 aattcacagg cggcaccact gagctgatca tcactccagt aatcgcatcc cggtccaacc
      781 ccgccagcgt aaacaaattc ctcgtcaaca cgccatattc atccacggtt tgctctcgcg
      841 ccgtgctcag ccgccaattg gccaccagct ccgtatcgcg aaaaaccccc agaaccgtgt
      901 tcgtattccc aacatcaatg gtcagcagca tattttccag ttcatctgca tatgcaattt
      961 accgaatctc tgtaatgtcg ccggcgatca ctgtcgttgt ctcgcccgta tcgcgcttca
     1021 ctcgcaacaa accttcttct tctaatcccg ctgttgtacc cgtgaaactt tctctcccat
     1081 tagtcactcg tacccgcttg ccccgcgcat agctcgaggt cgcttcgaac cgtttcacca
     1141 cactctttgc gccttcgtct agcagctcgc ggtaatcgcg ctcgaattgt cgcaggaatt
     1201 gcaccagcac ttccagtcgc gattgctgcc ggccagtctc gatccgcaaa gaagtgctct
     1261 tgctcgccaa gtcagcgggg aatttctcct ggttcacatt aatcccgatt cccacaatca
     1321 caaatcgcac ttgcgccggt tccgcatgca tttccgtcaa aatcccgccc acctttttcc
     1381 cgttgatcac caaatcattg ggccatttca aatcaacgtt caggcccgtc accccttgga
     1441 cggcggcatg cgccgacaac ccggccatca tcgtcagcag cggtgcctgc accggcgcta
     1501 gccgcggccg cagcaaaatc gtcgcgtaaa ttcccgtggc cctctcggaa tgccaagtgt
     1561 gcccagcccg gccacgccca gccgtttgct cttccgcgag caccaccgcg ccctccgcct
     1621 cactggcatg ccccaactcc aacgccacac gattcgtcga gtccgtctta aaaaaatgat
     1681 aaatccgctt cccaaataaa ctaccgcgca atcgctgccg caacatgtcc gccgtcaaaa
     1741 tatccggcac ttgttctaga aaatatcccg tggccgcgcg cccttttacc tttactccca
     1801 attcccgcaa tcgcgtgacc caccggaaca ccgtagaccg cgacacgccg atttcccgcg
     1861 caatcctcgc gccgcttatc acgatcgttc cattttccgc cagcagcgtc agcagcgcag
     1921 ccacctgccg ctccgtgctc ccagctatat ctttagaagg tttaacgcga actgccattt
     1981 ggagtgtcct gactcagcag cagccgtata tcttcaacta acaatctaat tgctcgaatc
     2041 gctccgtgag cttttgcatt tcgatgcgct gcgtcgcgag cgtcgcttcc aatcctcgaa
     2101 taatcttttc cggcgcgcgg cttcgaaacg tttcatcacc aagctgtttt tccttagacc
     2161 cgatggcttt gcgcagtcct tccagttcct tagtgatctt ggcgcactcc gccgcggcgt
     2221 ctaccgtttc cgcatactca atgcgcaaat caaatttcgc gctagaacgc accgcgccgc
     2281 tttgcgctag ggacccacca gtgatcttta aatccgtgag caagcctaac cgcacaatga
     2341 catcacgatt cgattccacc gccgcccgcg tcctttcatc agccaccgag agttccgccg
     2401 ccattttttt cttcggatcg agtttcatct ccgcacgaat attccgcact gccgtgatta
     2461 tttcctgcag cagtccaaat tcttccagcg ccgcattatt gcgccaactc tcccgcactt
     2521 ccggaaaacg ctccagcgca attgatttcg cacccgcttg ttgcggaaac tggtgccaca
     2581 attcttccgt caaaaacggc atgaatggat gcaacagccg cagcgccgcc tcgaacaccg
     2641 caaataaatt ccgccacgcg acaaccgcgc gctccttatc atcgctttgc aggtcaggtt
     2701 ttacccactc aatgtaccaa tcgcaaaaat caccccagaa aaattgatac accccttgcg
     2761 caccttcgtg aaaccggtaa ttcgccaatg cgtaattcat ttgctcggtg atcaccgcca
     2821 gccgcgcaaa aatccacgca tcccgcagcg gcacagcgcc cgtcagtgca taaggagcct
     2881 tcgcccgaat ctcaggagcc gccagttcct gtaatgtggc cccaccctgc tcaaactttt
     2941 ccaaatttac aaacagaaag cgcgccgcat tccaaatctt gttggcaaaa ttgcgcgcgc
     3001 ccgccagccg gtcatccgac agcacaatgt ccgtccctgg cgccgccatc gacgccagac
     3061 aaaaccgcat ggcgtccgta ccgtactgtt tattcagcaa taccggatca agtcccgtcc
     3121 ctttggactt ggacatcttc tcgccgctac cggtgcgcac cagcgagtgc aagtacaccg
     3181 cgcgaaaagg aacctgcccg gtaaagtgaa tccccagcat gatcatgcgc gcgacccaga
     3241 aaaacaaaat gtcgtatccg gtgatcagca aagtcgtcgg atagtaagtc ttgaggtcct
     3301 tcgtctcttc tggccacccc aaagtagaaa acggccaaag ccccgaacta aaccacgtat
     3361 ccaatacgtc cggatcctgc cgcagtttcc ccgatccgca cgttgcgcac ttcgcgggtg
     3421 tttcgcgcgc caccgtcacg tgcccgaatt cctcgcagta ccatgccgga atccgatgcc
     3481 cccaccacag ttgccgcgac aacgtccaat cgcgaatatt gcgcatccag ttaaaatatt
     3541 cttcccgccg attgtccggc acgatgcgaa tttcaccgcg ctccactgcc gcaatggccg
     3601 gctccgccag tggcttcatt ttgcaaaacc actgtgtcga cgcccgcggc tctacgatcg
     3661 tcctgcttcg ctcgcacaaa cccaccgcgt gcgtatgctc agtgactttt tctagtaatc
     3721 cctg
//
LOCUS       DATCBB010000582         2120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_213951, whole genome shotgun sequence.
ACCESSION   DATCBB010000582 DATCBB010000000
VERSION     DATCBB010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213951"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..474)
                     /locus_tag="VII37_06405"
     CDS             complement(4..474)
                     /locus_tag="VII37_06405"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5069397.1"
                     /translation="MFDNVRERTMRVARCIPPGKIDWTYAPAKFTLGDVLRHIAVTER
                     YMFAENVQNHESRYTSHGTELAADYESVIELMERLHAESLAIFAKLTDEDLKRKCKTP
                     GAAEIQTGKWLRAMVEHEVHHRGQIYLYLGLLGVPTPPLYGLTSEEVRAGSQAH"
     gene            complement(550..1875)
                     /locus_tag="VII37_06410"
     CDS             complement(550..1875)
                     /locus_tag="VII37_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5069398.1"
                     /translation="MATAITNRVDWKLIWAQRGFRYFFTAMFISLWGGGMNFAGVSWY
                     IMAATHSTVKVSLQVIVVTLPGLFIPFVGGVLIDRFDRRYLGVALDLARGVTVLATAY
                     IAWHGHLALWHLYLMTLVTGIASAMYWATVNALVQEVIPPSQFTAANAAVLIGVQSGL
                     LLAGAFVGFTYDRAGIAGILAIDGATYFVSAYCLYKVRRGYVSPREHQKYREYSEATE
                     ATAEALSTGENPEVAEAGISLAMYADLREGFQYLKQQPLVRALGITHSILMAGVVSAN
                     VVLVALTSDVLHAGARGLGYLESGWAAGAITGGLIATQLRQEVRLALYVAAFAGLALG
                     HMAAPFIAFLLAASLLQGLFGFCRALGGVVAQSALMSIVPRHFMGRTQSAMAIVTTAV
                     QLAMCVALGWIAERAGIIAGYTLLALLYAGATLTAFHARQLVRVAATES"
BASE COUNT          512 a          593 c          592 g          423 t
ORIGIN      
        1 actttagtgg gcctggctgc cggcgcgtac ttcttcagaa gttaagccgt acagcggcgg
       61 cgtgggcacg ccaagtaaac ccaaataaag ataaatttgg ccccgatgat gcacttcgtg
      121 ttcaaccatg gcccgcagcc atttaccggt ttgaatttcg gcagcacccg gcgttttgca
      181 tttgcgcttt aaatcttcat ccgtcagttt ggcaaaaatc gccaaagatt cagcgtgcag
      241 acgctccatc aattcgatca cgctctcgta atcggccgct agttcagttc cgtggctggt
      301 gtagcggctc tcgtgattct gcacattttc cgcgaacata tagcgttcgg tgacggcaat
      361 gtggcgcaaa acatcgccga gagtaaattt cgccggcgca taggtccagt cgattttgcc
      421 cggcggaatg cagcgagcga cgcgcatggt gcgttcgcga acattgtcga aaatttcaga
      481 aacgggccga tggtagtaat ttccataatg acctcatcga aattcgtgcc gttctcgaac
      541 gcgaaatttc taggattccg ttgcggcaac cctcaccagc tgccgcgcgt gaaaagccgt
      601 tagagtggcg cctgcgtaga gcaacgccaa caaagtgtag cccgctatga tgcccgccct
      661 ttcggctatc cagcccaggg ccacgcacat tgccagttgc accgcggtgg ttacgatggc
      721 catggccgac tgcgtgcgtc ccataaaatg gcgcggaacg atggacatga gcgcggactg
      781 cgctaccact ccgccgagcg cgcggcaaaa tccgaacaag ccttgcaata gtgacgctgc
      841 caaaagaaaa gcgataaacg gagcggccat atgtcccagc gcgagtccgg caaaagccgc
      901 aacgtacagg gcaaggcgca cctcttggcg caattgcgtg gcgatcaacc cgccggtaat
      961 cgcgccagcg gcccatccgg attccagata ccccagcccg cgcgcgccgg cgtgaagaac
     1021 atcgctggtc agcgccacga gcaccacgtt ggcactgacc actccggcca tcaggatcga
     1081 atgtgtgatt cccaaggcgc gcaccaaagg ctgctgcttg aggtactgaa aaccttcgcg
     1141 gaggtccgca tacatcgcga gagaaattcc cgcctcggcc acttccggat tttctcccgt
     1201 cgagagtgct tcggcagtag cttcggtggc ctcggaatat tcgcgatatt tttggtgctc
     1261 gcgcggcgaa acgtagccgc gccgaacttt gtacaaacaa taggcagaga caaagtaagt
     1321 ggctccatca attgccaaaa tgcccgcaat tcccgcgcga tcgtaagtaa aaccgacgaa
     1381 ggctccagcg agaagcagcc cgctctgcac gccgataagc accgcagcgt tggcggcggt
     1441 aaattggctc ggcggaataa cttcctgtac cagcgcgttg acggtggccc aatacatcgc
     1501 ggaggcgata ccggtaacca gcgtcatcag atacagatgc caaagcgcga gatggccgtg
     1561 ccaggcaatg tacgccgtcg ccaaaaccgt gacaccgcgc gcgagatcga gggctacgcc
     1621 taagtaccgg cggtcaaatc ggtcaatcag cacgccgccg acgaatggaa tgaacaggcc
     1681 cggcaaagta accacgatga cttgcaggct gacctttacc gtggaatgcg tggcggccat
     1741 gatgtaccaa ctcacgccgg caaaattcat gccaccgccc caaagagaaa tgaacatggc
     1801 ggtgaagaaa tagcggaaac cccgctgagc ccaaatgagt ttccagtcga cgcggttagt
     1861 gatcgctgta gccatggaac ggattagttt actattgtgg cggatatcgg cagcgcaaaa
     1921 gctgcgcgtt acgtgaattc aaatttgaaa tattgatcct tgaccgttgt tgcggatgga
     1981 aagtgcatct atgggaagcg atgtgaagtg ccgggtgcgc tttggcaaac aggaatcgga
     2041 gggcaaagcc ctgttagaga cgagcgaaat aatttttcgt ggtgatcttc gcttgaagat
     2101 tcccttcgcg acgattttgt
//
LOCUS       DATCBB010000583         5128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_343464, whole genome shotgun sequence.
ACCESSION   DATCBB010000583 DATCBB010000000
VERSION     DATCBB010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343464"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="asnB"
                     /locus_tag="VII37_06415"
     CDS             complement(<1..1067)
                     /gene="asnB"
                     /locus_tag="VII37_06415"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY5069399.1"
                     /translation="MDQAGQPKNLPVVDERPQNLCGITGFTSKNWEPSPNRIRTATST
                     LIHRGPDQTGVFESPLFSMGATRLKIIDLHGGDQPVLSEDGDTGIVFNGEIYNHLELR
                     SELEQRGHRFTSHSDTETVLHAFLEWDKDCFSRLRGMFAIALWCKSQKRIVLARDRMG
                     IKPLYISRKGEDLFFGSELKAIFAHPEIERSISLAGLDCYLSLNYVPCPWTLVDGIEK
                     LPPGHWLEWRDGKVTSDSYWRLPFGPSKHWTLDSAKEELDGLMQSALREHLLSDVPLG
                     VWLSGGIDSSAILHYAAQASSSQLKTFSISFRGRSFDETEYIHQVVKQYGTDHHEMDL
                     PPETNLRGAIEEFAYYADEPTA"
     gene            complement(1234..1701)
                     /locus_tag="VII37_06420"
     CDS             complement(1234..1701)
                     /locus_tag="VII37_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5069400.1"
                     /translation="MARAVLHAIELGQEDLTFWCGQLSEQELSATPSGLPSIAFHLRH
                     IARSLDRLLTYAEGRQLNERQLQALSSEAEPQCSRAEIFSELSAALTASSARILQLGE
                     KPDSLNQPRTVGRKNLPTTVAGLLVHIADHTQRHVGQAITTTKIVMAQRNQTL"
     gene            1924..2535
                     /gene="hpt"
                     /locus_tag="VII37_06425"
     CDS             1924..2535
                     /gene="hpt"
                     /locus_tag="VII37_06425"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY5069401.1"
                     /translation="MTTKQLKTHAAPRQAENVHESVRVLIPRERIKKRIKQLAHEIRR
                     DFGDQPLHLVGVLKGAVFFLADLARQIPGEVSFDFIAVSSYGDEMHSSGQVRLTRDLD
                     SSIEGKIVIVVEDILDTGMTMHYLLSIFQQRKPKHLRVAALLDKPERRITAVQADYVG
                     FRIPNEFVVGYGLDYAERYRNLLDVGILTIGKTEKRKRKGSKK"
     gene            2532..3224
                     /gene="deoC"
                     /locus_tag="VII37_06430"
     CDS             2532..3224
                     /gene="deoC"
                     /locus_tag="VII37_06430"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY5069402.1"
                     /translation="MSGQPTNSAGVAALIDHTLLKAEATRNDIARICKEALQYRFASV
                     CVNSVFVTQVSEALRNWPVKTCVVVGFPLGANLPAVKLDEAREAIEHGAQEIDMVIHI
                     GGLKGGDDAAVRSEIENLSRLVHERRAILKVILETALLSEEEKVRACRAARDAGADFV
                     KTSTGFSTSGAMIGDVELMRREVGREMGVKASGGVRTLDDLLNMVGAGATRIGTSNGV
                     KIVEEALAKFGV"
     gene            3304..5076
                     /locus_tag="VII37_06435"
     CDS             3304..5076
                     /locus_tag="VII37_06435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEY5069403.1"
                     /translation="MNPGQARVEVAPETTDTPLPAEVLATLTEIGEGVNSSLDLDEVL
                     AQTAALIKRHIDYEIFGVLMVEGGGAYLKHCFSIGYPRELVDNLRVPVGQGITGTAAA
                     TGYPVRVSDTSKDDRYIAAIENVRSELAVPLMLQGKCVGVLDIQSRHLDYFTPEQQSI
                     LTLLASRLAVAIENARLYQKERTQAETLLLLNEVGRETSSILDVEELLRRAAEQIKRV
                     IDYQILSLMLYDEKQKVFRHRIDVKHGQHVQGKLRVAVSEGIVGAAATLKAPVRVPDV
                     SADPRYLMVNPETRSELAIPMIHKGQVIGVLDLESPQLNYFTEDHVQTLSILAANLAV
                     SLENARLYEKLARDEARLERDLQAAKRIQGALLQPVPSEDYGLDMAARYLSAREVCGD
                     LYEFLRYGPQQLGIALGDVSGKGTAAALYGAVAIGIMRSLSPQKLQPADILKQMNQII
                     GERRIEGRFMTACFATWQKGKQKLRVANAGQSQPLLYKDGHCEKIELTGFPLGIFEEV
                     NYDEWNGVLEPGEILVFHSDGIAESTNPEGQFFGTEKLRKLIEQHHELQAAELADKIL
                     REVDWFTQNAPLADDRTLVIAKVR"
BASE COUNT         1181 a         1212 c         1572 g         1163 t
ORIGIN      
        1 gcagtcggct cgtccgcgta gtaagcaaat tcttcaatgg caccgcgtaa gtttgtctct
       61 ggcggcagat ccatttcgtg atgatcggtc ccgtactgct tgacgacctg gtgaatatat
      121 tccgtctcat cgaagctgcg gccgcggaag gaaatggaaa aagtcttaag ctgggaactc
      181 gacgcttgcg cggcgtaatg caaaattgcg gaagaatcga tgcctccact caaccacacg
      241 cccagaggaa cgtcggagag caaatgttct cgaagggcgc tttgcatcag gccatccagt
      301 tcttccttgg cggaatcgag cgtccagtgt ttcgatgggc cgaatggcaa acgccagtag
      361 gaatcagaag ttacttttcc atcgcgccat tccagccagt gacctggcgg aagtttttcg
      421 atgccgtcga cgagcgtcca cgggcaaggc acataattca gcgagaggta acaatccagg
      481 ccggcgaggc tgatgctccg ctcgatctcc ggatgcgcga aaatcgcttt aagctcagag
      541 ccaaagaaaa gatcttcacc cttgcggctg atgtatagcg gcttaatgcc catgcggtcg
      601 cgggccagaa cgatgcgctt ctgggattta caccacaggg caattgcgaa catgccacgc
      661 aggcgggaaa agcagtcttt gtcccattcg agaaaagcgt gcagaacagt ttcggtatcg
      721 gaatgcgacg tgaagcggtg tccgcgctgt tcgagttcgc tgcgcagttc gaggtgattg
      781 tagatttctc cgttgaagac gattccggta tcgccatcct cggaaaggac gggctgatct
      841 cctccgtgca ggtcaatgat cttgagacgt gtggcgccca tggaaaacaa tggggattca
      901 aagacaccgg tctggtcagg tccgcgatga atcagcgtac ttgtggcggt tctaatgcga
      961 tttggggaag gttcccaatt tttactagtg aagccggtta ttccacacaa attctgaggc
     1021 ctctcatcga caacgggcaa atttttcggt tggccggctt gatccaaacc cctggagatt
     1081 ggaggcgttt ggcgaaccgg cgatcgttcc gcctgtgacg gtagtgcaga agatcaatag
     1141 tgattcgatt ctatctgtgg aggttgcttg tgagcaatct cgtaatggct gtggaactga
     1201 atggaacgat gatgattatt tgtcaaacag gcgtcaaagg gtttggttac gttgggccat
     1261 gactatcttt gtcgtggtga tagcttgccc gacatgcctt tgggtgtgat ccgcgatgtg
     1321 caccagcagg ccggctacgg tggtcggcag attcttcctg ccgacagtgc gcggctgatt
     1381 tagagagtct ggcttttcgc cgagctgcag aattctggcg gaactggctg ttaaagccgc
     1441 tgacaactcg ctgaatattt cggcgcgaga acattggggc tcagcctcgc tgcttaatgc
     1501 ctgtagttgt cgctcgttca gttggcgtcc ttccgcgtat gtcagtaatc gatccaggct
     1561 gcgagcgatg tggcgaagat gaaatgcgat cgatggcaag cccgagggcg tggcggacaa
     1621 ttcttgttcg ctcaattggc cacaccagaa ggttaggtct tcctgtccga gttcgatggc
     1681 gtgcagaaca gcacgagcca cggcagggat ttctggatga gtgcctcgca gccagggttc
     1741 aggctgggat ttttgcatcg gtcacgctct ctgctaattt tgcttcacat gttttaaacc
     1801 tgcaattcgg atcgcacgca taaactggtc gaggctgctt agcataggcg aaccggcctc
     1861 ggtgcggctt gctctgatgg cgaggcgtgc gctagagtct cggttcggtt tgaatccgtt
     1921 tccatgacta caaaacaact gaagacccat gctgcgccgc gtcaagctga gaacgtacac
     1981 gaaagtgtgc gggtgcttat tccccgcgag cgcatcaaga agcgcatcaa gcaactggca
     2041 cacgaaattc ggcgagattt tggggatcaa ccgctgcacc tggttggagt attgaagggg
     2101 gcagtatttt ttcttgccga ccttgccagg caaataccgg gagaagtttc ttttgacttc
     2161 atcgcagtat ccagttatgg agacgaaatg cattcgtccg gtcaagtgcg gctgaccaga
     2221 gacctggact cgagtatcga aggaaagatt gtaattgtcg ttgaggatat tctggatacc
     2281 ggaatgacca tgcattacct gctgagtatt tttcagcagc gcaagcccaa gcatttgcgg
     2341 gtggccgcgc ttttggataa gccggagagg cgaatcaccg cagtgcaggc cgactatgtg
     2401 ggattcagga tacccaacga atttgtggtg ggctacgggc tggattacgc tgagcgttat
     2461 cggaacctgc tggatgttgg aatattgacg atcggaaaaa cggagaagcg aaagcggaaa
     2521 ggctccaaaa agtgagcggg cagcccacaa atagcgccgg tgtggccgcg ctgatcgatc
     2581 acacgctgtt aaaagcagag gctacgcgca acgacattgc gcggatttgc aaggaagcgc
     2641 tgcaatatcg atttgcatcg gtgtgcgtaa attctgtgtt tgttacgcag gtttccgaag
     2701 cgctgcgcaa ttggccggtc aaaacgtgtg tggtggttgg atttcctctg ggcgccaatt
     2761 tgccggcggt gaagcttgat gaagcgcgtg aggcgattga acacggtgcg caagagattg
     2821 atatggtgat tcacatcgga gggctgaagg gcggcgacga tgcggcggta cgaagcgaga
     2881 tcgagaattt gtcgcggctg gttcacgaaa gacgggcgat cctgaaagtc atcctagaga
     2941 cggcgctcct aagtgaagaa gaaaaagtgc gcgcttgccg agccgcacgt gacgcaggag
     3001 cggattttgt aaaaacgtcg actggcttca gcacgtcggg agcaatgatc ggggatgtgg
     3061 agttgatgcg tcgtgaggta gggcgcgaaa tgggcgttaa ggcttcgggc ggtgttcgca
     3121 cgctggacga ccttctgaat atggtgggcg cgggagctac gcgaattggc acgagcaatg
     3181 gcgttaagat cgtagaggaa gcgctggcga agtttggagt ttagtgcgcg gccgaatcgt
     3241 aactgattgt gaatacgtgg gatgccgggc ggcggttacc gcctgctata ataatgatac
     3301 acaatgaatc ccggacaggc acgggtcgaa gtggcgcccg aaacgactga tactccgctt
     3361 cccgctgaag tgctggcaac cctcaccgaa ataggcgaag gggttaactc gtccctggac
     3421 ctcgacgaag tgctggctca gaccgcggcg ctgattaaac ggcacatcga ttacgaaatt
     3481 tttggcgtgc tgatggtgga gggcggcgga gcttatctga agcattgttt ttcgatcggg
     3541 tatccgcgcg aacttgtcga caatttgcgg gttccggttg ggcagggaat taccggaaca
     3601 gcagcggcta ccgggtatcc cgtgcgcgtg agcgatactt ccaaagacga tcgttatatc
     3661 gccgcgatag aaaatgtgcg ctccgagttg gccgtgccgc tcatgctgca ggggaaatgt
     3721 gtaggcgtgt tggatattca gagccggcac ctcgattact tcacgccgga gcagcagagt
     3781 attttgacgt tgctagccag ccggctcgcc gtggcgattg aaaacgcccg cttgtatcaa
     3841 aaagagcgca ctcaagcgga aacactttta ttgctgaacg aagttgggcg cgagaccagt
     3901 tctattctgg acgtagagga attgctccga cgcgcggctg aacagatcaa gcgcgtaatt
     3961 gattaccaga tcctcagctt gatgctgtac gacgagaagc aaaaagtgtt tcggcatcgc
     4021 atcgatgtga agcacggcca gcatgtccag ggcaagttgc gcgtggcggt cagcgaaggg
     4081 attgtgggcg cggccgcgac gttgaaagca cccgtgcgtg tgccagatgt atcagcggat
     4141 ccgcggtatc tgatggtgaa tcccgagacg cgttcggagt tggcgattcc gatgattcac
     4201 aaagggcaag tcatcggggt gctggatctg gaaagtccac aactgaatta ttttaccgag
     4261 gaccacgttc agactctttc aattttggcg gcgaatcttg cggtgtcgct ggaaaatgcg
     4321 cggctgtacg agaagctggc tagggatgaa gcgcggttgg aacgggatct gcaagcggcg
     4381 aagcgcattc agggcgcgct gttgcagccg gtgcctagcg aagattatgg acttgatatg
     4441 gccgcccggt atctttctgc gcgtgaagtg tgcggcgacc tgtatgaatt tttgcgctat
     4501 ggaccgcagc agctgggcat agcgcttggc gacgtgagcg gcaaaggcac ggccgcggcg
     4561 ctgtatgggg cagtggctat cggcattatg cgaagtttat cgccgcaaaa actgcagcct
     4621 gcggacatcc tcaagcaaat gaatcaaatt ataggcgagc gccgcatcga aggccgcttc
     4681 atgactgcgt gttttgcaac ctggcaaaag ggcaagcaaa aattgcgcgt tgctaacgcc
     4741 ggtcaatccc agccgttgct ttacaaagat gggcactgcg aaaaaataga gttaaccgga
     4801 tttccgttgg ggattttcga agaagtgaat tacgacgaat ggaacggagt tctggagccc
     4861 ggggaaatac tggtttttca ttctgacggc atcgcggaga gtaccaatcc cgaagggcaa
     4921 ttttttggca ctgagaaatt gcgcaagctg attgaacagc accacgagtt acaagctgct
     4981 gagctggccg acaagatcct gcgcgaagtg gattggttta cgcagaatgc gccactggcg
     5041 gatgatcgaa ccctcgttat cgccaaagtt cgatgagcac tgccgcaccg cgatgctcca
     5101 atctgaaagc tgtagatcca accacgtt
//
LOCUS       DATCBB010000584         1371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_213999, whole genome shotgun sequence.
ACCESSION   DATCBB010000584 DATCBB010000000
VERSION     DATCBB010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213999"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VII37_06440"
     CDS             complement(<1..1246)
                     /locus_tag="VII37_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY5069404.1"
                     /translation="MKAAKHYVFLKRTKFAELLRSCVMVAVMLTAASSHGLVAVEAVA
                     AQEPGQNNQASPKPAAPLGTIKSIAGNTLVLTSDAGVEMIVDVRDSTKIVRIAPGQKD
                     LKDAQTIALTDLQVGDRILVRGKAADDNKTFAATSIIAMKKADIAEKQSHERDEWQKS
                     GVGGLVNNVDAAGGLISITTPSLREKKTVSVHVSKDTVLRRYAPGSVKFDDAKPAPLG
                     EIRPGDQLRARGEKNADGSELTAQEIVSGSFRNISGTISSLDNAQGTITVHDLATKKN
                     VAVKITSESQLRKLPAPMAQRIAQRLTGSSGTNGGANGDQAGSNQARSATSGTAAPAA
                     KEPAAGSGQGTGDFGGRARGAGAGAGGPGGGAMDLQQMINRMPASTIADLQKGDAVML
                     VGTEAGENAVAAITLLAGVEPIL"
BASE COUNT          254 a          432 c          342 g          343 t
ORIGIN      
        1 gcaaaatcgg ctcaaccccg gcaagcagcg tgatggctgc caccgcattt tcgccagctt
       61 cggtgcccac cagcattacc gcgtcgccct tctgtaaatc tgcaattgtg gacgccggca
      121 tacggttgat catttgctgc aaatccatgg caccgccgcc aggcccgccc gctccagcac
      181 cggcgcctcg tgcccgtccg ccaaaatctc ctgtcccttg tcccgaaccc gcagctggtt
      241 ccttcgccgc cggcgcagct gtaccggaag ttgcggaacg ggcctggttg gagcccgctt
      301 gatctccatt cgcgccgcca ttcgtgccac tcgatccagt caatcgttgc gcgattcgtt
      361 gcgccattgg cgcgggcaac ttgcggagct gcgactctga agtaattttc accgcaacgt
      421 tctttttcgt ggccaggtca tgcacggtga tagttccctg agcgttatcc agcgacgaaa
      481 tggttccaga aatatttcga aagctgccgg agacaatttc ctgcgccgtt agctcgctgc
      541 catccgcgtt tttctcaccg cgcgcgcgca attgatcgcc gggccggatt tcccctagcg
      601 gcgccggctt ggcatcgtcg aactttaccg atcccggtgc gtaccgacgt agcacagtgt
      661 ctttcgacac gtgcacggag accgttttct tttctcgtaa tgacggcgtt gtaatgctga
      721 tcagtcctcc ggcggcatcc acattattta ccagcccgcc gacgccggat ttctgccatt
      781 cgtcccgctc gtggctttgt ttttccgcga tgtcggcctt cttcatcgca ataatggaag
      841 ttgccgcgaa cgtcttattg tcgtccgccg ctttcccgcg taccagaatg cggtctccca
      901 cctggagatc ggtcagcgcg atggtctgcg cgtccttcaa atctttttga ccaggggcga
      961 tgcgaacgat ttttgtggag tcccgaacgt caacaatcat ttcaacgccc gcatcgctgg
     1021 tcagtactag agtattcccc gcaatcgatt taatagttcc cagaggtgcc gctggtttag
     1081 gggatgcttg attgttttgc cctggctctt gcgctgcgac ggcttccacc gcaacaagac
     1141 catgactcga cgctgccgtt aacatcaccg caaccataac gcacgaacgc agcagctcag
     1201 cgaatttcgt tcttttcagg aaaacgtagt gtttcgcggc cttcatattc cttttcaacc
     1261 tcaggcttcg tacgaggcac accatatctt tatcggttag acgcctcgct tgagcgaaat
     1321 cgtttcatgc tcttccaaca tcgcacaaaa acttgtcgcg agcgcaggaa t
//
LOCUS       DATCBB010000585         1125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_278920, whole genome shotgun sequence.
ACCESSION   DATCBB010000585 DATCBB010000000
VERSION     DATCBB010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278920"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1125
                     /locus_tag="VII37_06445"
     CDS             <1..>1125
                     /locus_tag="VII37_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY5069405.1"
                     /translation="VQWLMSAPLSRGQIEILAGNLTVGETYFFREKRTFEIFEEHVLP
                     ELTRLRRGGEQRLRLWSAACCSGEEPYSIAISLRKTLPDCAEWHVTILGSDINPRFLH
                     KASTGVFSEWSFRNAPPWLQERYFEKVGNGRWEILPEIKRMVSFSHINLAEDVYPSLL
                     NDTNAVDVIFCRNVLIYFAPEQVEKVIRNFHRCLTEGGWLVLSPSETSHVLFPQFVPV
                     NFDGAILYQKDSTRTRPVDRRAPQLEEARPVFFQPLPELVSETDATAPQVWAPRELAE
                     SKTAEPPPGAYDQAFALYEQGRYAETAKTLAALLASPPVEPRAMALLARALANQGNLT
                     EALGWCDKVIAADKLNPGAHYLRASVLHEQGNLNESAQSF"
BASE COUNT          229 a          323 c          335 g          238 t
ORIGIN      
        1 gtgttcagtg gttgatgtcc gcgccgttga gccggggcca gattgagatt ctggccggca
       61 acctcacggt cggcgaaacc tatttctttc gcgagaagag aacgttcgag attttcgagg
      121 agcatgtgct tccggaattg acgcggttgc gccggggagg cgaacagcgg ctgcgccttt
      181 ggagcgcggc ctgttgttcg ggcgaagaac cgtattccat cgccatttcg ctccggaaaa
      241 ccctcccgga ctgcgcggaa tggcacgtga cgattctggg cagcgacatc aacccgcgct
      301 tcctgcacaa ggcatcgaca ggtgtgttca gtgagtggtc atttcgcaac gcaccgccgt
      361 ggctccagga acgatatttc gagaaagttg gaaacgggcg ctgggagatt cttcccgaaa
      421 tcaaacggat ggtgagtttc tcccacatca acctggcgga agatgtttat ccgtcgttgc
      481 tcaacgacac gaatgcggtg gacgtcattt tctgccgtaa tgtgctgatt tattttgcgc
      541 ccgaacaggt ggaaaaagtc attcgtaatt ttcaccgttg tctcacggaa ggcgggtggc
      601 tggtgctcag ccccagcgaa acctcgcacg ttctgtttcc ccagttcgtg ccggttaatt
      661 tcgacggggc aattctctat cagaaagaca gcacccggac caggccggtg gaccggcgcg
      721 ccccgcagtt ggaagaagcc aggccggtgt tttttcagcc gctgccggaa ctcgtttcag
      781 aaacggacgc gacggcgccc caggtttggg cgcctcggga actggcggaa tccaaaacgg
      841 cggaaccgcc gcccggtgcc tacgaccagg ccttcgcgct ttatgaacaa ggccgctatg
      901 ccgaaacggc gaagacactc gctgctttgc ttgcctctcc gccagttgaa ccgcgggcca
      961 tggcgctcct cgcgcgggcc ttggccaatc aaggcaacct gaccgaggcg ctcggctggt
     1021 gcgacaaagt tattgcggct gataaattaa atcccggcgc ccattatctg cgcgccagcg
     1081 tcctgcacga acaaggcaac ttgaacgaat cggctcagtc gttca
//
LOCUS       DATCBB010000586         1073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_375902, whole genome shotgun sequence.
ACCESSION   DATCBB010000586 DATCBB010000000
VERSION     DATCBB010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375902"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VII37_06450"
     CDS             <1..281
                     /locus_tag="VII37_06450"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY5069406.1"
                     /translation="VFIRDGTRVFRTYFINSRGDEAMGSTWSYLDATPLGRQETWEDS
                     PEGYSQSAPYKWWNWHDNYAEASTDPKWVKVSDAGEAAFRKRDAKAKS"
     gene            complement(413..>1073)
                     /locus_tag="VII37_06455"
     CDS             complement(413..>1073)
                     /locus_tag="VII37_06455"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY5069407.1"
                     /translation="LLGMGAANHTLPATTWSTWSRTPFEFNGIRYISAMAPLFIHQYS
                     HAWFDFRGRRDHFADYFTNSVIATKVHKLWCLELAQQFPDYSEDLWGITASDSIRGYT
                     AWGGPPEMGRIDGSVVTCATAGSLPFLPQETLRVLRYMRENYAQKAWRNYGFVDAFNP
                     LTNWYSADVLGIDLGITILMAENARTGFVWEQFMKNDEAIRGMTRAGFHELVTPTPLN
                     S"
BASE COUNT          255 a          306 c          309 g          203 t
ORIGIN      
        1 atgtgttcat ccgcgacggc actcgtgtgt ttcgtaccta cttcatcaac agccgcggtg
       61 acgaggcgat ggggagcacc tggagctacc tcgacgcgac accgctcggc cgccaggaga
      121 cctgggagga ctcgccggag ggctactccc agagcgcgcc ctacaaatgg tggaactggc
      181 acgacaacta cgccgaggcc tccactgacc cgaagtgggt caaggtgtcc gacgctggag
      241 aagccgcctt ccgaaagcgc gacgcaaagg ccaaatcatg accgaggttc gcaagggggg
      301 cgccggcacg acgagcgtcg agcgaatcac ttctcgcgtt tctgtgtaat cagcacggag
      361 acaactctgc caagattagt gcatatcaat ttaaaaggct cacagagcct actcacgaat
      421 tgagcggtgt aggagtgact aattcgtgaa agcccgcgcg cgtcatgccc cggatggctt
      481 catcattttt catgaactgt tcccacacaa aaccagtgcg ggcgttttct gccataagaa
      541 tggtgatgcc caaatctatg ccgagcacgt ccgcgctgta ccagttagta agtggattga
      601 aagcatccac gaagccatag ttgcgccagg ctttctgcgc atagttttcc cgcatgtacc
      661 gtaaaacacg cagcgtctcc tgcggcaaaa atggcaagga gcccgcggta gcgcacgtaa
      721 ctaccgagcc atcgatgcgc cccatttccg gagggccgcc ccaggcggta tacccgcgaa
      781 tggagtcgga cgcggtaatc ccccacaagt cttcgctgta atcagggaat tgctgcgcca
      841 attcgaggca ccacagcttg tgtactttag tggcgatcac cgaattggtg aaatagtccg
      901 caaagtggtc gcgccgtccc cgaaaatcaa accacgcgtg agaatactga tggatgaata
      961 gaggcgccat cgcgctgatg tagcggatgc cattgaattc gaaaggggtc cgggaccacg
     1021 tggaccaagt ggttgcggga agcgtatggt tcgcggcccc catgcccaac agg
//
LOCUS       DATCBB010000587         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_83976, whole genome shotgun sequence.
ACCESSION   DATCBB010000587 DATCBB010000000
VERSION     DATCBB010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83976"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..743)
                     /locus_tag="VII37_06460"
     CDS             complement(129..743)
                     /locus_tag="VII37_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5069408.1"
                     /translation="MFVPKLLRDVPVQPTRRDFLKITTAGGVAAAVFGFDLQPAYAEL
                     RELKIARASETRSTCPYCSVSCGVIIYTIGDRAKNVTPQVVHVEGDPDHPINRGTLCP
                     KGASLQQDILNERRILKPQVRRPGSDHWEDISWDTALNEIAQKVKKTRDDSFIEKDAQ
                     GKTVNRCESIAWTGGCTDTNEFNYLVVKTMRALGVVHLENQARV"
     gene            1005..>2191
                     /locus_tag="VII37_06465"
     CDS             1005..>2191
                     /locus_tag="VII37_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5069409.1"
                     /translation="MATQPQSIKPDLGSLRIGDAHRKSGAGAGKRIWIIVLVLIPFAA
                     IAAATFAFRSQKPVVQVMTASKAEAGGRQSLLNASGYVTPRRRATIAAKITGRVTGVF
                     FDEGTHVKQGQLLATLDDSDVKRALESAKADRESAQAAIADYVVQLKNAKIQLGRAEQ
                     LQKAGVNTQENLDNASTAAESLNAKIALAKQQVVGADARIREAQQAVDNTVIRAPFDG
                     IVVSKDAQVGEMVSPISAGGGFTRTGIATIVDMNSNEIEVDVNESYIARVLPNQPVTA
                     TLDAYPDWEIPCKVRTVIPTADRQKATVKVRISFLKLDPKILPDMGVKVAFLAEEQPK
                     AKGPEPLTYIPKSAVRSEGGTSFVYLVHDGKVEHRAVKTGADRGTDVAIMAGVMPGDS
                     LVV"
BASE COUNT          476 a          549 c          685 g          481 t
ORIGIN      
        1 cagggatggt tttccgccgg gttaccgccc atgatcagca tcatgtcggt gttcttgata
       61 tccacccagc cgttggtcat tgcgccccgg ccaaacgttg gtcccaaact tgagaccgta
      121 ggggcgtgtc aaactctggc ctggttttcc aagtgcacga cccccagggc gcgcatggtc
      181 tttactacga ggtagttgaa ttcgttggtg tcggtgcagc cgccggtcca ggcgatggac
      241 tcgcaacggt tgaccgtttt tccttgagca tccttctcga taaaactatc gtcgcgggtt
      301 ttcttgacct tttgcgcgat ttcgttgagt gcggtgtccc acgagatgtc ttcccaatga
      361 tctgaacccg gcctgcggac ttgcggcttc agaatgcgtc gttcgttcaa aatatcctgc
      421 tgaagcgatg cacccttggg gcagagggtg ccacgattga tggggtggtc gggatcgccc
      481 tccacgtgaa cgacttgcgg ggtgacgttt tttgcgcgat caccgatagt ataaataatc
      541 acgccgcagc taacggagca gtacggacac gtggagcgcg tctcgctggc cctggcgatc
      601 ttcagttcgc gaagctcggc gtaggcgggc tgcagatcaa atccaaaaac cgctgcggcg
      661 acaccaccag cggttgttat tttgaggaag tcacgacggg taggctgcac gggcacgtcc
      721 cttagcagtt ttggcacaaa cacttgtgtt acaggtcctt aatagaacga tgtgacgctc
      781 ggagtcaagt aaacaattcc actttttagg atgcagtctg gagcgagacg gacacatact
      841 aaaattcgca agacaagtta caatatcgtt gcggcgggca tgcttcgcta tgcagccggt
      901 aatcttttac atctcgggcg cgtatcaata tacacagcac tttcgaagag ttgtctgcgt
      961 agcgactgac tacgcagttc ttggcccaag gagcatctga gcggatggcg acgcagccac
     1021 aatccattaa acccgaccta ggtagcctgc gtatcggaga tgcccaccgg aagtctggtg
     1081 ctggagccgg caagcgcatc tggatcattg tgctggtgct gattcctttc gcggccattg
     1141 ccgcggctac gttcgctttt cgaagtcaaa agccggtggt gcaggtgatg acggcttcga
     1201 aagcggaagc cggcgggcgc caatcgttac tgaatgcgag cggatacgtg acgccgcgca
     1261 ggcgtgccac catcgcggcc aagattaccg ggcgtgttac cggggttttt ttcgatgaag
     1321 gcacgcatgt gaagcagggg caattgctgg ctacccttga tgattcggac gtgaagcggg
     1381 cgctggaatc ggccaaggca gatcgtgaat cggcgcaggc ggcgattgct gattacgtgg
     1441 tgcagctgaa aaacgccaag attcaactgg ggcgagcgga gcaactgcag aaagcgggag
     1501 taaatacgca agaaaatctg gacaacgcat ccaccgcggc ggagagcttg aacgcgaaga
     1561 ttgcgctggc taaacaacag gtggtgggcg cggatgcgcg cattcgcgaa gcgcagcagg
     1621 cggttgacaa tacggtgata cgcgcgccgt tcgacgggat tgtggtttcg aaagacgcgc
     1681 aggtgggaga gatggtgtca ccgatttccg cggggggcgg atttacgcgc actggtatcg
     1741 cgacgattgt ggatatgaat tcgaatgaga ttgaagtgga cgtgaatgag tcttatatcg
     1801 cgcgggtgtt gcccaaccag ccggttacgg ccactctgga tgcttatccg gattgggaaa
     1861 ttccgtgcaa ggtacgcact gtgattccca ctgctgatcg gcagaaggcc acggtaaaag
     1921 tgcgcatctc gtttctgaag ctcgatccca agattttgcc ggatatgggc gtgaaggtgg
     1981 ctttcctggc ggaggagcag cccaaggcca aggggcctga gccgctcacg tacatcccga
     2041 aaagcgcagt gcgcagcgag ggcggaactt catttgtgta tctggtgcat gacggtaagg
     2101 tcgagcatcg ggctgtaaaa accggagcgg atcgcggaac ggatgtcgcg atcatggccg
     2161 gagtgatgcc gggagattct ctagtggtca a
//
LOCUS       DATCBB010000588         1042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_117660, whole genome shotgun sequence.
ACCESSION   DATCBB010000588 DATCBB010000000
VERSION     DATCBB010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117660"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VII37_06470"
     CDS             complement(<1..645)
                     /locus_tag="VII37_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY5069410.1"
                     /translation="MKVTNVRQAAELELSEHELSEIRMLIEERTGICFDQSRERFFVG
                     RVREHLLARGFERGTDFLRAARKANVEYEALLERLLTQETTFFRYPAVYEAFQKRALP
                     ELHIKKFWKTPRTLRIWSAGCSTGEEPYSIAITIADALTFADAWNVEILASDVGRVAL
                     KHAETAIYSGRSLSGVSEKQLATHFSPVKGGQQVKPRLRKMVTFTQMNLASAVYV"
     gene            complement(693..>1042)
                     /locus_tag="VII37_06475"
     CDS             complement(693..>1042)
                     /locus_tag="VII37_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069411.1"
                     /translation="KEVESGATLADQAGRALDAISNVVRQSAELVQEISLASKQQVRG
                     TEGVAHAMQIISSITRQTSQGTRGTVATVSQLVKLSDQLNDGLAQFRASSKHGNNAVQ
                     EESERQVPVGALR"
BASE COUNT          193 a          300 c          312 g          237 t
ORIGIN      
        1 cacgtagacg gcggaggcca ggttcatctg cgtgaaggta accatcttgc gtaaacgtgg
       61 ctttacctgc tggccgcctt tcacgggtga aaagtgcgtg gcgagttgtt tctcgcttac
      121 gccggaaagg ctgcgcccgg aataaattgc ggtttcagcg tgtttgagcg ccacgcggcc
      181 tacgtcgctg gccaggattt cgacattcca ggcatcggcg aacgtcagcg catcggcgat
      241 ggtgatggcg atggaataag gttcttcgcc ggtagagcaa cccgcgctcc agatgcgcag
      301 cgtgcgggga gttttccaga atttcttgat gtgcagttcc ggcaacgcgc gcttttgaaa
      361 cgcttcgtaa acagcgggat agcggaagaa agtggtctcc tgggtaagca aacgctcgag
      421 cagcgcttcg tattccacgt tggccttgcg cgcggcgcgc agaaaatcag tgcctcgttc
      481 gaatcctcgc gccaacaggt gttcgcgcac acgtccgacg aagaatcgct cacgcgattg
      541 gtcgaaacaa atccccgtcc gctcttcgat tagcatgcgg atctcgctca actcgtgttc
      601 gctaagttcc agctcagctg cctggcgcac gttggtaact ttcacgcttg gtcattccgt
      661 tcggccccgg cgcttgcccg agcgggaaat atttaccgca atgcgccgac cggaacctgc
      721 cgctcggatt cttcctgtac agcgttattg ccgtgtttcg acgatgcgcg gaattgcgcg
      781 agcccgtcgt tgagctggtc agacaacttg accaactggc ttaccgtcgc caccgtgcca
      841 cgcgtgccct gagacgtttg gcgggtgatg ctggaaataa tctgcatggc gtgcgctacg
      901 ccttccgtac cgcgcacttg ctgcttggag gccagtgaaa tttcctggac aagttcggcg
      961 gattggcgca ctacgttgga gatggcgtcc agagcacggc cggcctgatc ggcaagcgtt
     1021 gcgccgcttt ccacttcctt gg
//
LOCUS       DATCBB010000589         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_278966, whole genome shotgun sequence.
ACCESSION   DATCBB010000589 DATCBB010000000
VERSION     DATCBB010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278966"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VII37_06480"
     CDS             <1..263
                     /locus_tag="VII37_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069412.1"
                     /translation="LPHVAQAALRDGWVDVVALGRMALSYPGVLWDASAGNTLQRNKV
                     CRTFSDCTTAPRKGLPSGCYPLDAYYKNSEHAAPLAAAKAKA"
     gene            346..1311
                     /locus_tag="VII37_06485"
     CDS             346..1311
                     /locus_tag="VII37_06485"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF017951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5069413.1"
                     /translation="MSIEAFDKRRVVRRKVEGIAAALLPYEVDGRIAVDAFQRHVIAT
                     HRAGLVNAVNMDTGYVNYLSEAEKREVLQWTREALGNDTPFVAGAYIEGQDGHVVALY
                     RRQMDAIVEMGATPILFQTARLHGKSAREKIAVYAAAAQGYKSVLAFELGRMFAPNGE
                     IFDRETFLGILNIPEITGMKHSSLHRVTELERLALRDEHRPDFRIYTGNDLGVNMIEY
                     GSDYLLGLATFAPEKFAERDRMWQSGDVEYFALSDALQHLGNVAFRAPVPAYKHSAAV
                     FLHEVGRVPSNLTHTKSPQRPAWEAEIMRECAQRLGFEGAGRTAP"
     gene            1350..2291
                     /locus_tag="VII37_06490"
     CDS             1350..2291
                     /locus_tag="VII37_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY5069414.1"
                     /translation="MTDEGSSRRTFLRNATLTGATLALSRRLPAAEKRSVLLFTKSSG
                     WEHDVVKTKDGNPSIVERAVRELGAKHGFEVTATKDGRIFDSKEFRAHSALLFFTTGD
                     LTTPGTDKQPPMSESGKQAMLAAIHDGLGFVGVHAASDTFHTKPDPEDRSNRYVAHGE
                     QSDAYLRMLGGEFIVHGSEPRLQTADLIVNDANFPGLGGVKSPVAFNEEWYSLKDLVP
                     DIHVILTLDTKGMQGECYQRPAYPVTWARMDGKGRVFYTAMGDRPENWQDTFFLNVLG
                     GGIRWSIGDAKATLNKNLTEVAPGYATIPPKNAPAKK"
     gene            2326..>2646
                     /locus_tag="VII37_06495"
     CDS             2326..>2646
                     /locus_tag="VII37_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069415.1"
                     /translation="MKNSITIAALAAFALAAGIKAQSGVTKAPSESRASATAGANYDW
                     PVYGGAPENNHYSPLAQIHRGNVARLKVAWNFDTEEEGGLQTSPIVVHGVLYGITPTQ
                     KIFAL"
BASE COUNT          603 a          636 c          854 g          553 t
ORIGIN      
        1 ttctgccgca tgtggcacaa gcggcgctgc gggatggttg ggtggacgtt gtggctcttg
       61 gccgcatggc actctcttac ccgggagtgt tgtgggatgc ttcggcgggg aacacgctgc
      121 agcgtaacaa ggtgtgccgc acattcagcg attgcaccac cgctccgaga aaaggtttgc
      181 cgtccggatg ctacccgttg gacgcgtatt acaagaactc ggagcacgcg gcgccacttg
      241 ccgcggcgaa agcaaaggca tgattctcgt ttggagtcgg gtgggccgct cgcgctggtc
      301 caacttggga tttaaggatg ctgccgattt aagttccata agggtatgag catcgaagca
      361 ttcgataaac gacgcgtcgt tcgccggaaa gttgaaggta ttgcggcagc gctgctgccc
      421 tatgaagttg acggacgcat tgccgtagat gcgtttcagc ggcacgtgat cgctacacat
      481 cgtgccgggc tggtgaatgc cgtgaacatg gacaccggct atgtgaatta cctgagtgaa
      541 gcggaaaaga gagaggtgct gcagtggacg cgcgaagcgt tgggaaacga tacgccgttt
      601 gttgctggag cgtacattga gggacaagac ggccacgtgg tcgctttgta ccggaggcag
      661 atggacgcca tcgtggagat gggagcgacg cccatactct ttcagaccgc tcgattgcac
      721 ggaaaatcgg cgcgcgagaa gatcgcggtg tatgcggcgg cggcgcaggg atataagagt
      781 gtgttggcgt tcgaattggg ccgcatgttt gcgccgaatg gcgagatttt tgatcgagaa
      841 acgtttctcg gtatcctcaa tattccggag attaccggga tgaaacactc ttcgctccat
      901 cgagttactg aattggagcg gctcgcgcta cgggatgaac accgcccgga ctttcggatt
      961 tacacgggca acgatctggg cgtcaacatg attgaatatg ggtcggacta tttattggga
     1021 ctggcgacgt ttgctcccga gaaattcgcg gagcgggacc gaatgtggca gagcggggac
     1081 gtggaatatt tcgcattgtc ggacgcgctg caacatctgg ggaacgtagc atttcgtgcg
     1141 ccggtgcccg cttacaagca ctccgccgcg gtcttcttgc atgaagttgg ccgggttccg
     1201 tcgaatttga cgcacacaaa aagtccgcag cgtccggcgt gggaagcgga aataatgcga
     1261 gagtgcgcgc agcgcttggg atttgaaggt gccggcagaa cggcgccgtg aagagggcaa
     1321 gttgcaatag ctatccggga gaaccgcaga tgaccgacga aggatcaagc cgcagaactt
     1381 ttcttcgcaa cgcaactctg acaggggcaa ctttggcgct atctcggcgg ctaccggccg
     1441 cggaaaagcg ctcggtgtta ttgttcacaa agtcgtcggg ctgggaacac gatgtggtga
     1501 agaccaaaga tggaaatccg agcatcgtgg agcgcgcggt tcgcgaactg ggggcgaaac
     1561 acggctttga agtgacggct acgaaagacg gaagaatttt tgattcgaaa gagtttcgag
     1621 cgcattcggc gttgctgttt tttacgacgg gcgatctaac cacgccgggg acggataaac
     1681 agccgcccat gtcggagagc ggaaaacagg cgatgctcgc tgctattcac gacggacttg
     1741 gatttgtagg tgtgcatgcc gcgagcgaca cgtttcacac caagcctgat ccggaggacc
     1801 gttcgaacag atatgtcgcg cacggagagc agagcgatgc gtatctgcgc atgctgggcg
     1861 gagaattcat tgttcacggc agcgagccac ggctgcagac cgcggacctg attgtaaacg
     1921 atgcgaattt tcccgggctg ggtggagtga agtctccggt ggcgttcaat gaggaatggt
     1981 attcgttaaa agatttggta ccggatatcc acgtgattct gacgctggat acgaagggca
     2041 tgcaaggcga atgctaccag cggcctgcgt atcccgtgac gtgggcgcgt atggatggca
     2101 aaggccgggt tttttatacg gcgatgggcg accgtccgga gaattggcag gatacatttt
     2161 ttctaaatgt gcttggtgga ggaattcgct ggtcgatcgg ggacgccaag gccacattga
     2221 ataaaaacct gacggaggtt gcgcccggct acgcgaccat tccgccgaaa aatgcaccgg
     2281 caaagaaata accattgttc tttgcggttc tgaggcaagg agccagtgaa aaactcgatt
     2341 accattgctg cgctcgcagc ctttgcgctt gcggctggaa ttaaggccca gtccggcgtt
     2401 acaaaagcgc catcggaaag cagagcatcg gcaacggccg gagcgaatta cgattggccg
     2461 gtctacggcg gagcgcctga aaataatcat tactcaccgc tggcgcagat ccatcgcggc
     2521 aatgttgcgc ggctcaaagt tgcatggaat ttcgacacag aagaggaagg cgggttgcag
     2581 acgagcccca tcgtggtgca cggggtgttg tatggcatta caccgacgca aaaaatattc
     2641 gcgctg
//
LOCUS       DATCBB010000590         4360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_375970, whole genome shotgun sequence.
ACCESSION   DATCBB010000590 DATCBB010000000
VERSION     DATCBB010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375970"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2211)
                     /gene="acnA"
                     /locus_tag="VII37_06500"
     CDS             complement(<1..2211)
                     /gene="acnA"
                     /locus_tag="VII37_06500"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY5069416.1"
                     /translation="MLERTERMAISAGNSFGTRDTLRVGAQSFEIHRLEALEKNRVAN
                     LGKLPFSLRILLENLLRCEDNRFVHAPDIQSLANWRPGTPEKEIAFMPARVLLQDFTG
                     VPAIVDLATMREAVQRMGGNPKRINPLFPAELVIDHSVQVDSFGSVNSFGLNAELEFQ
                     RNVERYAFLRWGQTAFHNFKVVPPDTGICHQVNLEYLARVVCATAKGNKIEAYPDSVV
                     GTDSHTTMINALGVFGWGVGGIEAEAAMLGQPSSMLVPEVVGFRLHGKLNEGATATDL
                     VLTVTEMLRKHGVVGKFVEFYGSGLSTLSIPDRATIANMAPEYGATMGFFPVDAETLD
                     YLRFTARPVEQIALVEAYCKEQMLFRTDATPAPDFNGTLELDLGTVESTVAGPKRPQD
                     RVALRNAKNSFAKVVEGAPSIHATVKNNGDSFDLSSGAVVIAAITSCTNTSNPSLMLG
                     AALLAKKAVERGLSVKPWVKTSLAPGSKVVTDYLHAAGLTPYLEKLNFHLVGYGCTTC
                     IGNSGPLPEAIGAAIKDNNMVAVAVLSGNRNFEGRIHPLVRANYLASPPLVVAYALAG
                     RMDVDLTTESLGSDTKGQPVYLRDIWPTPQEIEKTVRQAVTSEMYSKQYAQVFEGDAH
                     WKSMPVPAGDIYQWDPKSTYIKMPPYFDNMPKEAPALTDVRGARVLAILGDSVTTDHI
                     SPAGSIPVDSPAGKYLIAHGVKPHEFNSYGARRGNHEVMMRGTFANIRLRNQLAP"
     gene            2274..3755
                     /locus_tag="VII37_06505"
     CDS             2274..3755
                     /locus_tag="VII37_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEY5069417.1"
                     /translation="MSTTLKIVLISTYELGRQPFGLASPAAWLRKRGHSVVCMDLARQ
                     ALNELAIKEADLIAIYLPMHTATRLAAQILPSLRAENPSAHFCCYGLYAPMNEAYLRS
                     LGVATILGGEFENGLVHLAERLSQSNRSNGNGIARSAPQEESLISLERLRFEVPDRSG
                     LPAMQKYAHLIIPGDGYRVVGSTEASRGCKHLCRHCPIVPVYNGVFRTVPREIVLEDV
                     RRQVGAGAQHISFGDPDFFNGIGHAIPLVEQFHRECPDVTYDVTIKIEHLRKYEKHLP
                     KLKETGCLFVISAVESVNDVFLAALEKGHTRDDFMYVAATFRALGLTLHPTFVPFSPW
                     TSVDNYLDLLRVLYDEQLMENVAPIQLGIRLLIPEGSRMLELEEIRKVIGPFDAQSLV
                     YPWRHSDSRVDALSETVQEIAAAADRDKLSRSTAFERIWIAAHRAAGIEAPALLSTRT
                     ARAVPFLSEPWYCCAEPTTDQLISIGRPKPVVSNPTVSADSFV"
     gene            3778..>4360
                     /locus_tag="VII37_06510"
     CDS             3778..>4360
                     /locus_tag="VII37_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5069418.1"
                     /translation="MENSSKEPGSKPRRLLLFTNKLGYQTRSFEEAAQKHAVEIIYVT
                     DRCHQLEDPWGDRAIAVHFESAQTAAANVVEALKGKSVDGIVALGDSPSVAAAYGARE
                     LGIFYNHPAAVEACRSKLRMREVFRGAGLKTPWFRTINLDPLPEPTLLGISYPCVLKP
                     LSLSASRGVIRANNRDEFIAAAQRIRKLLESPEI"
BASE COUNT          946 a         1151 c         1250 g         1013 t
ORIGIN      
        1 gggagcgagc tggttgcgca gccgaatatt cgcaaacgtg ccgcgcatca tgacttcatg
       61 attgccgcgg cgcgcaccgt acgagttgaa ttcgtgcggc ttgacaccgt gggcgatgag
      121 atatttaccc gccgggctgt cgacgggaat ggagcccgcc ggggaaatgt gatcggtggt
      181 cacgctgtcg ccaagaattg ccaatacacg tgctccacgc acgtcggtaa gcgcaggcgc
      241 ctcctttggc atgttgtcga aatacggcgg catcttgatg tacgtggatt tgggatccca
      301 ttggtaaatg tcgcctgcgg gcacaggcat ggatttccaa tgcgcgtcgc cctcaaaaac
      361 ctgggcgtat tgcttgctat acatttctga agtgacggcc tggcgaacgg ttttttctat
      421 ttcctgagga gtgggccaga tgtcgcgcag gtaaacgggt tgacccttgg tgtcggaacc
      481 caacgattcg gtggttagat ccacatccat tcgtccggcc agcgcatacg cgaccaccag
      541 cggcggtgat gccagataat tcgcacgtac cagcggatga atgcgtcctt caaaattgcg
      601 attgccgctg aggactgcga cagccaccat gttgttgtct ttgatggccg cgccgatagc
      661 ttcgggcaga ggcccgctat tgccgatgca agtcgtgcag ccataaccga ccagatgaaa
      721 gttcagtttt tcaaggtaag gcgtgaggcc agcagcatgg agataatcag tgaccacttt
      781 tgatcccggg gcgagactgg ttttaaccca cggttttacg cttagtccgc gttcgactgc
      841 ttttttcgcg agcaatgcgg cgcccagcat caacgaagga tttgaagtgt tggtacaact
      901 ggtgatcgct gcaatcacta ccgcgccgct ggatagatca aaggagtcac cattattttt
      961 gaccgtggca tgaattgacg gcgcaccttc gacaactttt gcgaaggagt tcttcgcatt
     1021 gcgtagcgct acgcgatcct gcggccgctt cgggccggcc acggtggact ctacagtccc
     1081 gagatcaagc tccagcgtgc cgttgaaatc cggtgcgggg gttgcatcgg tgcggaagag
     1141 catttgttct ttgcagtagg cttcgacgag cgcgatctgc tcgacaggcc gcgccgtgaa
     1201 tcgtagataa tccagcgtct ctgcgtcgac ggggaagaaa cccatggtcg cgccgtattc
     1261 gggggccatg ttggcaatgg ttgcgcggtc tggaatgctc agggtggaaa gtcccgagcc
     1321 ataaaattcc acaaattttc cgaccactcc atgtttgcgc aacatttctg ttaccgtgag
     1381 caccaggtcg gtggcggtcg cgccctcatt cagtttgccg tgcaagcgaa atcccactac
     1441 ttcgggaacc agcatcgagg aaggctgccc taacatggcg gcttctgctt caatgccacc
     1501 aacgccccat ccaaagacgc cgagtgcgtt aatcatggtg gtgtgcgaat cggtgcccac
     1561 tacggaatcg ggatacgctt caattttatt tcccttggcc gtcgcgcaaa ctacgcgcgc
     1621 gaggtattcg agatttacct ggtggcaaat cccggtgtct ggcgggacta ctttgaaatt
     1681 gtgaaatgca gtttggcccc aacgcaggaa ggcatagcgc tccacattgc gttgaaattc
     1741 caattcggca ttcagtccga aggaatttac gctgccaaaa ctatcgactt gcaccgaatg
     1801 atcaatgacc agctccgcgg ggaacagcgg attgatacgc ttgggattcc cgcccatgcg
     1861 ctgcaccgct tcacgcatgg tggccagatc gacgatggcc ggcacgccgg tgaagtcctg
     1921 taagagcacg cgcgcgggca tgaaggcaat ttctttttct ggagttccgg gacgccaatt
     1981 ggccagcgat tgaatatccg gggcgtgaac gaacctgttg tcttcgcaac gcagtagatt
     2041 ttcaagcaga atacgcagag aaaaggggag cttgcccaaa ttcgctacgc gatttttctc
     2101 gagcgcttcc aagcggtgta tttcgaagct ttgcgcgcca acgcgtagcg tgtcacgcgt
     2161 gccaaacgaa tttcctgcag agatggccat cctctcggtc ctttcaagca ttgcccagcc
     2221 ctccatgata accgaaatcg aattacagcg cgggcggcta tactggtggt ggcgtgagca
     2281 ctactttgaa aatcgtctta atttccacct atgagttggg gcgccagcct tttggcctgg
     2341 cttccccggc ggcctggctg cgtaaacgcg ggcatagtgt tgtgtgcatg gatctggcgc
     2401 ggcaggcgtt gaatgagctt gcaattaaag aagctgactt aatcgctatt tatctgccta
     2461 tgcacacggc gacgcggttg gcagcgcaaa ttcttccgtc gttgcgcgcg gaaaatccga
     2521 gcgcgcattt ttgctgttac gggctctatg cgcccatgaa tgaggcgtac ttgcggagcc
     2581 ttggtgtcgc aacgatctta ggcggcgagt ttgaaaatgg gttggttcat ttggcggagc
     2641 ggttgtcgca gtcgaaccgg tcgaacggga atggcatcgc tagatcagcg ccgcaggaag
     2701 aatctttaat ttcgctggaa cggttgagat ttgaggtgcc ggaccgctca ggattgcccg
     2761 caatgcagaa atacgctcat ctcattattc ctggcgatgg ttaccgcgtt gttgggtcca
     2821 cggaagcaag ccgcggatgc aagcatctgt gccgccactg cccgatcgta ccggtatata
     2881 acggcgtttt caggaccgtg ccacgagaaa ttgtactcga ggatgtacgc cgccaagttg
     2941 gcgcgggtgc gcaacatatt tcgttcggcg acccggactt ttttaatggt ataggccacg
     3001 ccattccgct cgtcgaacaa tttcaccgtg aatgtcccga cgtgacttat gacgtgacca
     3061 taaaaatcga gcacctgaga aagtacgaaa agcatttgcc taaactgaag gaaactggtt
     3121 gcttgtttgt gatttcggca gtcgagtctg taaacgatgt attcctcgcg gccctggaaa
     3181 aaggccacac gcgcgacgat ttcatgtatg ttgcggccac atttcgtgct ttggggctga
     3241 ccctgcatcc cacgttcgtt cctttttcgc cgtggactag cgtcgataat tatctggatc
     3301 tgttgcgcgt cttgtatgac gagcagctca tggaaaatgt ggcgcccatt caactgggta
     3361 ttcgcctgtt gattccagaa ggctcgcgca tgctggaact tgaagaaatc agaaaagtga
     3421 ttgggccttt tgacgcgcaa tcgctagtgt atccgtggcg ccactctgat tcgcgagtgg
     3481 acgcgttgag cgagacggta caggaaattg ctgctgcggc cgaccgagac aagctgtcgc
     3541 gctcgactgc ctttgaacgc atttggatag ccgcacatcg agccgcggga atcgaggcgc
     3601 cggcgctttt aagcactcga accgcgcgag ccgtgccatt cctcagcgag ccctggtatt
     3661 gctgcgcgga gccaacaacg gatcagctga tttcgatcgg ccgcccaaaa ccggtggtca
     3721 gcaatcccac cgtttcggcg gacagctttg tttgacgccg cgagaattta attaacaatg
     3781 gaaaattcat ccaaagagcc tgggtccaaa ccacgacgat tgcttctgtt cacgaataag
     3841 ctgggctacc agacacgcag ctttgaagaa gctgcccaaa aacacgccgt tgaaatcatc
     3901 tacgttacgg accgttgtca ccaactggaa gatccgtggg gcgatcgagc gattgcggtg
     3961 cactttgaat ccgcgcagac cgcggcggct aatgtggttg aggcgctgaa aggcaaaagc
     4021 gtcgatggaa tcgtggcctt gggagactca ccgtcagtag ctgcagcgta cggcgctcgt
     4081 gagctgggaa tcttttacaa tcatccagct gcggtggaag cctgccgttc aaaattacgc
     4141 atgcgcgaag tgttccgcgg agcgggattg aagacaccat ggtttcggac catcaatctc
     4201 gatccactgc ccgagccgac attgctgggg atttcttatc catgcgtgtt gaagccgctt
     4261 tcgctttcgg ctagccgtgg cgtgatacgc gcgaataatc gcgacgaatt tatcgcggcg
     4321 gcacaacgca tacgcaagct gctcgaatcg ccggaaattc
//
LOCUS       DATCBB010000591         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_214130, whole genome shotgun sequence.
ACCESSION   DATCBB010000591 DATCBB010000000
VERSION     DATCBB010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214130"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VII37_06515"
     CDS             complement(<1..266)
                     /locus_tag="VII37_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069419.1"
                     /translation="MHTSQILQAWGKILKGEHPALSIEITRECPLRCPGCYAFDDAHL
                     GGGQTLRDLNDRKGQALIDGVLEVVDSLKPLHLSIVGGDPLVRYR"
     gene            700..>2054
                     /locus_tag="VII37_06520"
     CDS             700..>2054
                     /locus_tag="VII37_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5069420.1"
                     /translation="MVEFRDWAPKSFLVLRHYSWKQFAADLLAGITVGLVALPLAMAF
                     AIASGVPPQLGLYCAVIAGFLISALGGSSTQIGGPTGAFVVVVFGIVTRYGLEGLYLC
                     TLMAGVFLLVLGATGLGTAVKFIPRPVVVGFTNGIAVIIASAQIKDFFGLKIEHVPGE
                     FWARLETLAKNFGTISYTETMLGIWALIVILFCVRRLPKVPGYIVALFVGTAVVWVFK
                     LPVETIGSRFGGIPSGLPVLNIPAFHFSHLRPLIGPAITVAMLGAIESLMSAVVSDRM
                     SGDKHNPNVELVAQGIANIVSPLFGGLPATGAIARTATNIRSGARTPVAGMVHALTLL
                     AILLFAAPMAKFIPLTVLAAILFVVAYNMGEWREIGSIVRLGPADRSVWFITFALTVM
                     ADLTVAVEVGMALAALLYIYRVAQTTTVGVLTPADVEDARPHQLQGQQVPPYVTILRI
                     HG"
BASE COUNT          418 a          538 c          607 g          491 t
ORIGIN      
        1 cgataacgga ccagcgggtc gccacccaca atggagagat gcagcggttt caaggagtcc
       61 actacttcca gaacgccgtc gattagagct tgtcccttgc gatcgttgag atcccgcaag
      121 gtctggccgc cgccaagatg cgcgtcatcg aacgcgtaac agcctgggca tcgcaatggg
      181 cattctcgag tgatttcaat agaaagcgcc gggtgttcgc ctttgagaat ctttccccaa
      241 gcttgaagaa tttgagaggt gtgcatcttt aatgatccct ttgagaagca cgtttacatt
      301 tcttaaattg aattgttcag cgagcctcac cagagcgagc gggacgccga tgttttctgc
      361 gaagcatacc ccgcgtgcct acagtcgaca taccgtaaat tggaaagtgt ggagcgagct
      421 gctccgcaag caacaccctt tccttgtcat ttggatgcgc gaactttggc aggagtcacc
      481 cgatccagga agaatacacc agacctcccg tctaatcttc tgtaatgaaa agcaagcgta
      541 tatcaggcaa cggcgtaacg ggtttggaat acgcatcaga aaaatagtct gccgtggagg
      601 ttgcgggtga aagaatggtt gcacgacaac gattgcatgc ccttccgagt gggtcgctca
      661 gtaaagtgaa atcgcgattt aaagatcaga tggataacaa tggttgaatt tagagactgg
      721 gctcccaagt ccttcctggt tttgcgacat tattcctgga agcagtttgc ggctgaccta
      781 cttgcgggca tcaccgtggg gttggtggcg ctgccgctgg ccatggcttt tgccattgct
      841 tcgggcgtgc ctccgcaact gggattgtat tgcgcggtga tcgcgggatt tttgatttcg
      901 gcattgggag gatcgtcaac gcaaatcggc gggcccactg gcgcgttcgt tgtggtggtg
      961 ttcggcatcg taacgcgcta cggccttgaa gggctgtatt tgtgcacgtt aatggcgggc
     1021 gttttcctgc tagtgctggg cgcgacgggt ctgggcactg cggttaaatt tattccgcga
     1081 ccggtggtgg tgggattcac caacgggatc gcggtgatta tcgccagcgc gcaaatcaag
     1141 gacttcttcg ggctgaaaat tgagcatgtg cccggagaat tttgggcgcg gctggaaacg
     1201 ctggccaaaa acttcggcac tatttcctat accgaaacga tgctggggat ctgggcgctg
     1261 atcgtaattc ttttttgtgt gcgacgactg ccgaaggtgc cgggatacat cgtggcgttg
     1321 tttgtgggca ccgctgtggt gtgggtattc aagctgccgg tggagaccat cgggagcaga
     1381 ttcggcggaa tcccttcagg attgccggtg ctgaatattc cggcgttcca tttcagccac
     1441 ctacgcccgt tgatcggccc ggccattacc gtggctatgc ttggcgccat tgaatcactg
     1501 atgtcggcgg tggtttctga tcgcatgagc ggcgacaaac acaatcccaa cgtggagttg
     1561 gtggcgcaag gcatcgcaaa tattgtgtcg ccactctttg gcggtttgcc tgcgacggga
     1621 gcaatcgcgc gaacagccac aaacattcgt tcgggtgcac gaacccctgt tgcggggatg
     1681 gttcatgcgc tgacgctgct ggcgattctt ttgtttgcgg cgccgatggc taaattcata
     1741 ccgctaacgg tgttggcggc aattttattt gtagtggcct acaacatggg cgagtggcgc
     1801 gagatcggct ccatcgttcg cctgggcccg gccgaccgct ccgtgtggtt catcaccttc
     1861 gccctaaccg taatggctga cctcaccgta gccgtggaag tgggcatggc gctagccgcc
     1921 ttgctctaca tctaccgcgt ggcgcaaacc accaccgtcg gcgtcctcac tccagcagac
     1981 gtagaagacg cgcgtccgca ccaactgcag ggccaacagg tgccgcccta cgtcaccatc
     2041 ctgcgcatcc acgg
//
LOCUS       DATCBB010000592         1113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_376000, whole genome shotgun sequence.
ACCESSION   DATCBB010000592 DATCBB010000000
VERSION     DATCBB010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376000"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="VII37_06525"
     CDS             <1..>1113
                     /locus_tag="VII37_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEY5069421.1"
                     /translation="LASTSNPVGGQVQAAPRGIFEISKLNLTGRGDSLSLKLRGSTIE
                     DRALLAYSDPNTFGHRNLSMQATAYTEKTQDINTFTATRYEGSLQLTDQVTPRTTLIF
                     RYVFRKVLVSNIADTINPVAIPLFEQPTLVSQFGVTWVHDRRDNPADATRGSYNSADF
                     GVAGSNIGSSASFLRFYYQNSTYHPIKRRFSFARSIRIGGLIPYGDTVSLTFPLPTAT
                     PLPTVIPLPERLFAGGGTSLRGFALNQAGPRDSVTGFPVGGQALLVLNQEFRFPMRLP
                     YFGTSLGGAIFYDGGNVYSRLSRISFRSSTPAPTFDTSNPATATCLTNCSNELNYFAH
                     TVGFGFRYATPVGPIRIDLGYQINRPTFVIPCTTLTG"
BASE COUNT          238 a          331 c          266 g          278 t
ORIGIN      
        1 cctggccagc acttcgaatc ccgtcggcgg tcaggtgcaa gccgcaccgc gaggaatttt
       61 tgagatatca aaattaaatc tgaccggccg cggcgattcg ctgtccctta aattgcgggg
      121 cagcactatt gaggaccgcg ccttattggc ctattccgat ccaaatacct tcggccacag
      181 gaatttaagc atgcaagcta ccgcatacac ggaaaaaacg caggacatca acacctttac
      241 cgcaacgcgt tacgaaggat cgctacaact cactgaccag gttactccgc gcaccactct
      301 tattttccgt tacgtatttc gtaaagtatt agtctccaac atcgccgaca ccattaatcc
      361 tgtggccatc ccgctgtttg agcaacccac gctagtgtcg caatttggtg tgacttgggt
      421 tcatgaccgt cgcgataatc ccgcggatgc cacgcgcggc agctataaca gtgcggattt
      481 tggcgtggcg ggcagtaaca tcggatcgag cgcaagtttt ttacgattct attatcaaaa
      541 ttctacctac catccgatta aaagacgttt cagcttcgca cgctccattc gcatcggcgg
      601 gctgatcccc tatggcgaca cagtttcgct gacatttccg ctgccaaccg ccacaccgtt
      661 accaacggtt attccgctgc ccgagcgtct ttttgcaggt ggcggaactt cgttgcgtgg
      721 gtttgctttg aaccaagcgg gcccgcgtga ttccgttacc ggctttcctg tgggcggtca
      781 ggccttgctg gtgctgaatc aggagtttcg tttccccatg cgcctgcctt acttcggaac
      841 gtccctgggc ggcgccatct tttacgacgg cgggaatgtt tacagcagat tgagccgtat
      901 cagctttaga agctcaacgc ctgcgccgac ttttgacacc agcaaccccg ccaccgccac
      961 gtgtttgaca aactgcagca acgaactgaa ttattttgct cacacggtcg gctttggctt
     1021 ccgttatgcc acgccagtcg gtccaattcg catcgacttg ggctaccaga ttaatcggcc
     1081 aacctttgtg attccatgta ccactctcac cgg
//
LOCUS       DATCBB010000593         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_84032, whole genome shotgun sequence.
ACCESSION   DATCBB010000593 DATCBB010000000
VERSION     DATCBB010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84032"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VII37_06530"
     CDS             complement(<1..209)
                     /locus_tag="VII37_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5069422.1"
                     /translation="MMLREAGVDSRNEPRQEILQAAARLFQQQGYDATSMNDVAAALK
                     LSKGGLYHHFQSKDEILFHIMSHAM"
     gene            305..1255
                     /locus_tag="VII37_06535"
     CDS             305..1255
                     /locus_tag="VII37_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY5069423.1"
                     /translation="MPNKVPINPVLVVHGGAWDIPDEMVEAHLNGVRNAAAAGWRTLE
                     GGGSALDAVEEAVVVLEDDETFDAGRGSFLNRDGKVQLDAFIMDGATLRGGGVGCVER
                     LRNPVRAARKILSDSPHVYFVAEGAERFAEEHGITLCRNEDLIIPREVDRLRAYQSQI
                     EASGDGKDLFAPAIAHDLLSHDTVGAVALDRDGNIAASTSTGGTLNKAPGRLGDSSLI
                     GCGCYADNLSAAASTTGWGEPIMKLVLAKWAADRVESGSLPEWVAEEAMNYLQQRLNG
                     HGGIILLDAKGRFGIAHNTPRMAWAIHTVEKQETGIQRDS"
     gene            complement(1366..>2326)
                     /locus_tag="VII37_06540"
     CDS             complement(1366..>2326)
                     /locus_tag="VII37_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY5069424.1"
                     /translation="AMIHKVFALSKDKIENQRSFLSLFDELPRRRLEIRFGKFSMPDF
                     FDVNSVGADTHFQFMNWSVDNNGAWDYAADTRGYTVGIVADYEDRNWGFRFAETLMPK
                     VANGIDLVWRPWQVHAENFEYELRHGVLPKKSGVIRLLAYTNYANMGIYRDANMQFKE
                     GLVPVPQITNHPWHITHKYGFGVNLEQNLTRNFSAFARWGWDDGKTESFAYTEIDSTF
                     NQGIGANGALWRRKQDRAGVAFVSNGIKKDHQVYLADGGLGFLLGDVHLTYGRENIVE
                     SYYTAHIWRGIYIAPGVQHLNNPGYNRDRGPVVVPTMRAHVEF"
BASE COUNT          493 a          631 c          674 g          528 t
ORIGIN      
        1 tccatcgcgt gggacatgat gtgaaacaga atttcgtctt tcgactgaaa gtggtgatac
       61 aggccgcctt tcgaaagctt gagcgccgca gcgacatcgt tcatcgaggt ggcgtcgtat
      121 ccctgctgct ggaaaaggcg ggcggcagcc tgcaggattt cctgccgagg ttcgttgcga
      181 gaatcgacgc cagcttcgcg aagcatcatc agatggtatc gcggaatggg gtttgcactc
      241 tagcccgctc gccatttgcc gcagctgcta gacttatttc cgcgacttcc tgcaaggact
      301 gaaattgcca aataaagtgc caatcaatcc cgttctagtt gttcacggcg gtgcctggga
      361 cattcctgat gagatggtcg aagcccatct caatggagtg cgcaatgccg ctgctgctgg
      421 atggcggacg ctggaaggcg gcggttccgc tctcgatgcg gtcgaagaag ctgtggtcgt
      481 tctcgaagac gatgagactt ttgacgctgg ccgcggcagt tttctgaacc gcgatggcaa
      541 agtgcaactc gacgctttca tcatggacgg ggcgactttg cgcggtggcg gtgtcggttg
      601 cgtcgagcgg ctgcgcaacc cggtgcgcgc cgccagaaaa attttgagcg acagcccgca
      661 cgtatatttc gtcgccgaag gggctgagcg ctttgccgag gagcacggca tcactctttg
      721 ccgcaacgaa gatttaatta ttccccgcga agtcgaccgc ttgcgcgctt atcagtccca
      781 gattgaagct tcaggcgacg gcaaagatct gttcgcgccg gctattgcgc acgacctgct
      841 ttctcacgac accgtgggag ctgtggcgct cgaccgggac ggcaacattg cggcttcaac
      901 gtcgactggc ggcactctca ataaagctcc gggccgcctc ggcgattctt cgctgatcgg
      961 atgtggatgc tatgccgaca atctcagcgc ggccgcttcc actacgggat ggggcgaacc
     1021 gatcatgaag ctggtgcttg caaagtgggc ggcggaccgc gtggaatcag gcagtttgcc
     1081 ggagtgggtg gcggaggaag ccatgaacta tctccagcag cgtctgaatg gccacggcgg
     1141 aattattctg cttgatgcta aaggccgctt cggaattgcc cacaacacac cgcgcatggc
     1201 ttgggcaatt cacaccgtag aaaagcaaga gacgggcatt cagcgagata gttgaacagg
     1261 taaaagaggt gtttcgccga tgtgccctgt cccagtgtcc cctgtgaaat ctctgtgtcc
     1321 cctgtggtaa agattttgac tttcggtgag agctgagaac tctcttcaaa actccacatg
     1381 cgcccgcata gtcggcacaa caaccggccc gcgatcgcgg ttatagcctg gattgttgag
     1441 gtgctgtact ccgggagcga tataaattcc gcgccaaatg tgggctgtgt aatacgattc
     1501 gacgatattc tcgcgcccgt aggtcagatg gacatcgccc agcaggaaac ccaacccgcc
     1561 atccgccaga tacacctggt gatctttctt gatgccgttt gacacgaagg caacgccggc
     1621 gcggtcttgc ttgcgacgcc agagcgcgcc gttagcgccg atgccctgat tgaatgtgga
     1681 atcaatttcc gtgtaagcga acgattcggt ttttccgtcg tcccaacccc agcgggcgaa
     1741 ggcgctgaag ttcctggtga gattttgttc cagatttacg ccgaacccat acttgtgggt
     1801 gatgtgccag ggatggttcg tgatctgcgg gaccggcacc aggccttctt tgaattgcat
     1861 attcgcatcg cggtagatac ccatgttggc gtagtttgta taagcgagca gccggatcac
     1921 tcccgatttt ttaggcagca cgccatgccg cagttcgtat tcaaaatttt ctgcgtgaac
     1981 ctgccacggc cgccaaacca ggtcgattcc atttgcgacc ttcggcatta gcgtttcggc
     2041 gaaacgaaaa ccccagttgc gatcttcata atcggctacg atgccgacgg tgtagccgcg
     2101 ggtatcggct gcgtaatccc aagcgccatt gttgtccacc gaccagttca tgaattgaaa
     2161 gtgcgtatcg gcgccgacgg aattgacatc gaagaaatcc ggcatactga acttgccaaa
     2221 gcgaatttcc agacggcgtc gcggcaattc gtcgaaaagc gacaggaaac tgcgctggtt
     2281 ctcgatcttg tccttactca gcgcgaagac tttgtgaatc atggcg
//
LOCUS       DATCBB010000594         1506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_278997, whole genome shotgun sequence.
ACCESSION   DATCBB010000594 DATCBB010000000
VERSION     DATCBB010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278997"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1242
                     /locus_tag="VII37_06545"
     CDS             304..1242
                     /locus_tag="VII37_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069425.1"
                     /translation="MRSNLATLRSDVELSLAGRVVSPFGYRDRKSVETVLTGIPEIDA
                     LAGGFPRGALTEICGPPCSGRTTVLLSALASRTAEAEVCALIDARDSFDPRSAEAAGV
                     ELQQLLWVRCRSLDQSLRAADLLIQGGGFGFIALDLSDVAPETVRHVPLNAWFRFRRA
                     VEDTSTVLLVLEQESNAKTCASLVLRMSMKAATWSGRAESDVHDKTAVINHAGISRHS
                     QLLAHECLGQKFLARGCLPHARLLDGSDVQAEMLHTRMQSINFRQTQNGPVSIERHFG
                     GRDLTDHSDDNIQSFATQSIWSYLQALPVEAKARLK"
     gene            1302..>1506
                     /locus_tag="VII37_06550"
     CDS             1302..>1506
                     /locus_tag="VII37_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069426.1"
                     /translation="MSFAAIHIPDFIVQAVVRGEEALRERAVAIVEGTAPLWSVVAAN
                     RRAAQAGIELGMAKSQAAQFTGVE"
BASE COUNT          316 a          320 c          492 g          378 t
ORIGIN      
        1 cattgaattg cgtgccagag atgccgtacg actggccttg gccaaccacg gtaggcgcgg
       61 atgtcaagca aaaatcggga aaattgaatg ctcggaagaa gtcgtgcgtt ggttggtggt
      121 ggcagcgggg atatttttta aaaatcttta gttcaatgca cgatagtgtt gcgcggtttg
      181 tgtctttcga aattttatcc actaaagtta aaagtaaaag gcttgacagg ggcgcgggaa
      241 ttttacagaa tacgctaggc gaacaaaaag cgaatatcgt tttggggttc ccactatcct
      301 gtcatgcgct ccaatctcgc tactctccgt tctgatgtcg aactttccct tgctgggcgg
      361 gtggtttctc cgtttgggta tcgcgaccgc aagagcgtgg aaacagtgct taccgggatt
      421 ccggagattg atgctctggc cggaggcttt ccgcgtgggg cgttgacgga aatttgcggg
      481 ccgccgtgtt ccgggcggac tacggtgctg ctttcggctt tggcgtcgag gacggcggag
      541 gcggaagtgt gcgcgttgat tgatgcgcgt gattcgtttg atccgcgtag cgcggaagct
      601 gcgggggtgg aactgcagca attgctgtgg gtgcgctgcc ggagtcttga tcagtctttg
      661 cgggctgcgg accttttgat tcagggcggc gggtttggat tcattgcgct ggatctaagt
      721 gatgtggcgc ctgaaactgt gcggcatgtg ccgttgaatg cgtggtttcg gtttcggcgc
      781 gcggtggaag atacgtccac ggtgttgctg gtgctggagc aggagtccaa cgcgaaaacg
      841 tgtgcttcgc tggttttgcg gatgagcatg aaggcggcga cgtggtcggg cagggcggaa
      901 agcgatgttc acgataagac cgcagtaatt aaccacgcag gaatttcgcg gcattcccaa
      961 ttgttagcgc atgaatgttt ggggcaaaag tttttggcac gcggatgttt gccgcatgca
     1021 cgattgctgg atggctcgga tgttcaagcg gagatgctgc atacgcgcat gcagtcgatc
     1081 aattttcggc aaacacaaaa tgggccagtt tctattgagc gacattttgg cgggcgcgat
     1141 ttaacggatc atagtgacga caatatacaa agttttgcga cgcaatcgat ctggagctat
     1201 ctgcaagcct tgcctgtgga agcgaaagcg agacttaaat gaggagcgcg ggccgcaaat
     1261 aattttgctg aattttactt ccaattcgcg attgaaccgt catgtctttt gccgccattc
     1321 atattcccga ttttattgtg caagccgtgg tgcgtggcga ggaggcgctt cgcgagcgtg
     1381 cagtagcgat tgtggaaggt acggcgccgt tgtggagcgt ggtggcggcc aatcgacgag
     1441 cggcgcaagc gggaatcgaa ttgggaatgg cgaaatcgca agcggcgcaa tttacgggcg
     1501 tagaaa
//
LOCUS       DATCBB010000595         1390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_245975, whole genome shotgun sequence.
ACCESSION   DATCBB010000595 DATCBB010000000
VERSION     DATCBB010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245975"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1352)
                     /locus_tag="VII37_06555"
     CDS             complement(240..1352)
                     /locus_tag="VII37_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5069427.1"
                     /translation="MKAISGKILTVSVVTLLACALVLSSFAQAPAGPQGGQPQQSAPP
                     TPPPPAQNPASKPPTQAPPEAGVTIAVDVPVVTVDVIATTKNGDILTGLKKENFRVLD
                     DGAPQTITNFAPTDAPITMVVLMEFSGRAYGWFAAYAKYWADALFPNLQQKDWVALET
                     FDMKTRLEVDFTQDKNEVRAAINHLYFPGFSESNVFDAILETNERLKDVKGKKSILLL
                     ASGVDTFSKHTLDQTMKSLRQSDVTIFAVGLDKTFTNYLDLHGSRGAHMDYLQAENQL
                     KTFATETGGFSWFPQFDGEIPGIFQQVAAMLRHQYSISYTPGNGAKDGRFHKVKIELV
                     APDGGPLTVLDQKGKKQKYVVYAREGYQAPKNGVGD"
BASE COUNT          307 a          404 c          333 g          346 t
ORIGIN      
        1 aatttattta tcaccccgca caccagcgcc ctcagcgaca ggttatggcg ccgcgagacc
       61 gctatgttgg tggatttact ggagcgctgg ttcgacggcc gcgaactttt caatcgcgtc
      121 gatttcgcgc gcgtctactg aacttgcctc aaccagcggc tactgaagtg cctgaaaaag
      181 atctgcggct aaaaaacacg cacgcggttt tctttggaaa tgtaattcgg cgattcgatt
      241 tagtccccga cgccattctt tggagcctgg tacccttccc gcgcgtaaac cacgtatttc
      301 tgtttcttgc ctttttggtc gagaacggta agtggaccgc cgtcgggagc caccaattcg
      361 attttcacct tgtgaaagcg cccgtccttc gcaccgtttc ccggagtgta agaaatgctg
      421 tactgatggc gcagcatcgc agccacttgc tgaaagattc ccggaatttc gccatcgaat
      481 tgcggaaacc acgaaaatcc gccggtctca gtcgcaaatg tctttaactg attttccgcc
      541 tgcagataat ccatgtgcgc gccgcgcgat ccatgcaaat ccagataatt cgtaaacgtt
      601 ttgtccagcc ccacggcgaa aatggtcaca tcgctctggc gcaacgattt catggtctga
      661 tccagcgtgt gtttggaaaa agtatccacg ccgctggcca gtagcagaat cgattttttt
      721 cctttcacat ccttcaaccg ttcattcgtt tccagaatcg catcgaaaac atttgattcg
      781 ctgaagcccg ggaaatatag atgatttatg gccgcgcgaa cttcattctt atcctgcgta
      841 aaatccactt ccagccgcgt cttcatgtca aacgtttcaa gcgctaccca atctttttgc
      901 tgaaggttgg gaaaaagcgc gtcggcccaa tacttggcgt aagccgcaaa ccagccatac
      961 gcccgcccgc tgaactccat aagcaccacc atcgtgatag gcgcatccgt aggcgcaaaa
     1021 ttcgtaattg tttgcggcgc cccatcgtcc agcacgcgaa aattttcttt cttcaggcca
     1081 gtcaatatgt cgccattttt tgtcgtagcg ataacatcca cggtaactac cggaacgtcc
     1141 accgcgatcg tcaccccagc ttcgggaggc gcctgcgtcg gaggcttcga cgccggattc
     1201 tgcgcgggcg ggggaggcgt gggtggcgcg ctctgttgcg gctgcccgcc ctgcggcccc
     1261 gcaggcgctt gcgcaaatga tgaaagtacc agtgcgcatg ccagcagcgt aacgacgctc
     1321 accgtcaaaa ttttgccaga gatagctttc atatctgcat ccccattttt atgtcgccca
     1381 tttctctaaa
//
LOCUS       DATCBB010000596         1647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_474142, whole genome shotgun sequence.
ACCESSION   DATCBB010000596 DATCBB010000000
VERSION     DATCBB010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474142"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..665
                     /locus_tag="VII37_06560"
     CDS             333..665
                     /locus_tag="VII37_06560"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5069428.1"
                     /translation="MKDQERRRLPRYNLRTPLRFRALGVAADTSEHFTEALNVSRGGF
                     FFASSAPLRVGMQLEVTLRMPVEVTGTVAKETNCTARVTHTRSEAFGDGRIGFGAQIE
                     EHTDGWVD"
     gene            complement(717..>1647)
                     /locus_tag="VII37_06565"
     CDS             complement(717..>1647)
                     /locus_tag="VII37_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY5069429.1"
                     /translation="KEWSPWSGPYPHSGAEMEIPPARFAQWKAVIHGGQPGDGVDWAS
                     LAYFPRNVAPVIDGIAIQEPGVRANATNIMAANQPTTVTLKLPPAPNTSGVIITQSGT
                     PPRFEPPPQGIQQKGYATVLWTAHDDNEDELRYAVYYRGENEQDWKLLKDKLEQKFYS
                     WDTTSFPDGAYYLKIVATDAPSNPSATALTAERISERFEVDNTPPVVEHLQAIAVVTS
                     SGAIQLTGIIVKFIARDAGSSVERAQYSLDGADWILISPAGNISDAPEEHYEFTISDT
                     SAGEHTIAVRAYDRFENVGSAKTTVTVTNSNHR"
BASE COUNT          336 a          399 c          517 g          395 t
ORIGIN      
        1 tgagtacggg cgtgcgatgg atttttgaag cgatgcgtgc gtgagcttgc ttgatggagt
       61 cccacgaagg aggcgcaaaa ttttctgcgg ttacagtttt ttgagtaggg tccgagtgcg
      121 cgagattttt tgaatttgtg agagccatta ttggtagtcc aatgcacagc agaatactgg
      181 aagcggtagt tggcgcaaaa tgattaggct tggctgcgcg agactagtgg gcgctaattt
      241 gtcccttgac gtgccccgca gctttgctac gctgacttga accccgcaag tgagactcgc
      301 tagccggttt tccccgcgtg gagcaagaca aaatgaaaga ccaagagcgc cggcggttac
      361 cccgctacaa cctgcgcacg ccattgcgct ttcgggcgct gggcgttgcc gcggatacca
      421 gtgaacattt tacggaagcg ctgaatgttt cgcggggagg attttttttc gcttcctcgg
      481 caccgctacg ggtcggaatg caactggaag ttacgctgcg catgcctgta gaggtgactg
      541 ggaccgtggc gaaggaaaca aattgcacgg cgcgagtgac tcacacgcga agcgaagctt
      601 ttggcgatgg acgaatcggt tttggagcgc agattgaaga gcacacggat ggctgggtcg
      661 attaagacga aataatagcg gtgcagcttg cgggcgataa gttattcggg cgatagttat
      721 cggtggtttg aattcgtgac ggtcacggta gtttttgcac ttcccacatt ttcaaagcgg
      781 tcgtaagcgc gcaccgcgat ggtgtgttct ccggcggacg tatcgctaat cgtaaattca
      841 tagtgttcct cgggcgcgtc gctgatgttt cccgcgggag atatgagtat ccagtctgcg
      901 ccgtcgagac tgtattgggc gcgctccaca ctgctgccgg catcgcgagc tataaacttt
      961 acaattatgc cagtgagctg aatagccccg ctgcttgtaa caaccgcgat agcctgaaga
     1021 tgctcgacga cgggtggagt gttgtcgact tcgaagcgct cactgattcg ttccgcggtg
     1081 agcgcggtgg ccgatgggtt ggacggcgca tcggtggcga caatcttcag gtaataagcg
     1141 ccatcaggga aggacgtggt gtcccaggaa taaaatttct gctccagttt gtcctttaac
     1201 aatttccaat cctgctcatt ttctccgcgg taatacaccg cgtagcgcaa ttcatcttca
     1261 ttgtcgtcat gcgcggtcca caggacggta gcgtagcctt tttgttgaat gccctgcggg
     1321 ggcggctcaa atcttggcgg ggtgcccgac tgggtgatga tcacgccgga agtattcggc
     1381 gccggaggga gtttgagagt gacggtggtt ggctggttgg cggccattat atttgtggcg
     1441 ttggcgcgga cgccgggttc ctgaatggcg atgccatcaa ttaccggcgc gacattgcgc
     1501 gggaaatacg cgaggctggc ccaatcgacg ccgtcacccg gctggccgcc gtgaatcacg
     1561 gctttccatt gcgcaaatcg agccggggga atttccatct ccgcgccgga gtgtgggtaa
     1621 ggaccagacc aaggggacca ttctttg
//
LOCUS       DATCBB010000597         1097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_149781, whole genome shotgun sequence.
ACCESSION   DATCBB010000597 DATCBB010000000
VERSION     DATCBB010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149781"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="uraD"
                     /locus_tag="VII37_06570"
     CDS             <1..447
                     /gene="uraD"
                     /locus_tag="VII37_06570"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY5069430.1"
                     /translation="GARRWASAMVALRPSGSVVELSEAADHVWGAMKEADWMEAFACH
                     PRIGERPPRRTAHATAKSVAWSSQEQSSAASAAEKVLTELAEGNARYEERYGFTYIVC
                     ATGKSAEEMLTILNRRLLSDRAAELIEAAEQQRQITQIRLGKWLIE"
     gene            444..785
                     /gene="uraH"
                     /locus_tag="VII37_06575"
     CDS             444..785
                     /gene="uraH"
                     /locus_tag="VII37_06575"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY5069431.1"
                     /translation="MTGRMNAITTHVLDAVLGKPAAGITVRLEKRERFLWNVISSGVT
                     DGDGRCRDLAPGAKKGVYRLTFETRAYLAHNGRESIYPEISITFVCSGKEHYHLPLLL
                     SDNSYTTYRGS"
     gene            789..>1097
                     /locus_tag="VII37_06580"
     CDS             789..>1097
                     /locus_tag="VII37_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069432.1"
                     /translation="MAKLGGNRYGKSRVRLSRIMRHGDRHDFNEWSVHVLLEGDFESS
                     YTEADNSNVLPTDTMKNTVYYVARGSKAATIEEFAMELGDYLLANNAQVSGVKVEIEE
                     R"
BASE COUNT          254 a          288 c          360 g          195 t
ORIGIN      
        1 ggcgcgcggc gctgggcttc ggcaatggtt gcgctgcgcc cgagcggcag cgtggttgaa
       61 ctgagcgagg cggcagacca cgtttggggc gcgatgaagg aggccgactg gatggaggcc
      121 ttcgcatgcc atccgcgcat cggcgagcgt ccgccgcggc ggaccgcaca cgctacggcg
      181 aagtcggtcg cgtggtcgag ccaggaacaa tcatctgccg cgagcgcggc ggagaaagtg
      241 ttgacggagc ttgccgaagg aaatgcgcgc tacgaagagc gctacggttt tacctatatt
      301 gtgtgcgcca ccggaaagag cgcggaagag atgctgacga tcctaaatcg ccgcctgttg
      361 agcgaccgcg cggcggaatt aatcgaggcg gctgaacagc aacgccagat cacacagatt
      421 cgtctgggaa agtggctaat tgaatgactg gccgaatgaa cgcgattacg acacacgtgc
      481 tggatgcagt tcttggtaag ccagccgccg gcatcacagt ccgtctcgaa aaacgggaac
      541 gctttctgtg gaacgtaatt tcgtccggcg tcacggacgg ggacggacgt tgccgcgatc
      601 ttgcaccggg agcaaagaag ggcgtctaca ggctcacctt tgagactcgg gcctacctgg
      661 cgcacaacgg ccgcgaaagt atttatccgg agatttcaat cacctttgtc tgcagcggca
      721 aggaacacta ccacttgccg ctgctgttga gcgacaacag ctacacaact tatcgaggaa
      781 gctgagagat ggctaagctg ggtgggaatc gctacggaaa atcgcgcgtg cggctatcgc
      841 ggattatgcg ccacggagac cggcacgact tcaacgaatg gagtgtgcac gtgctgctgg
      901 aaggggattt tgaatccagc tacacggaag cggacaacag caacgtgctg ccaaccgaca
      961 ccatgaagaa caccgtgtat tatgtggctc gcggctccaa ggcggcgact atcgaagaat
     1021 ttgcgatgga gttgggcgac tatctgctcg caaataacgc gcaggtctcc ggggtcaagg
     1081 tggagattga ggagcga
//
LOCUS       DATCBB010000598         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_117777, whole genome shotgun sequence.
ACCESSION   DATCBB010000598 DATCBB010000000
VERSION     DATCBB010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117777"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /gene="serA"
                     /locus_tag="VII37_06585"
     CDS             complement(<1..1133)
                     /gene="serA"
                     /locus_tag="VII37_06585"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY5069433.1"
                     /translation="MKVIVADKISARGVQLLKEQPGWNVVLTTKDNLVAEIADAQALI
                     VRSATKVSSDLLDKAPNLRCVGRAGVGVDNIDLEAATKRGIIVMSTPGGNAVSVAEHT
                     FALMLSLARQVPRLDKAFHEGRWEKSSAAGMELSGKTLGLIGLGRIGSEVALRAEAFD
                     MRVLGYDPYISEAAAKEFSVELVPLERLLAESDFVSLHTALSPATQNLINAESLAKMK
                     KGVFIINAARGELIEEAALADALKSGHIGGAGLDVFAVEPPKNSPLVGLPNVVGTPHI
                     AGSTTEAQEEVGTQVAVQVKDYLAEGIIRNAVNLPVLSAEQYRRVRPYMELAERLGSL
                     VSQASDTRPSRIRIRYAGEVSEVGTHLLRNAVLAGVLNAVLDE"
     gene            1306..1761
                     /locus_tag="VII37_06590"
     CDS             1306..1761
                     /locus_tag="VII37_06590"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY5069434.1"
                     /translation="MSLSERIQKDLVDAMRAKDELRLSVLRGMKAAIKHKEVEKIRVL
                     DETESIQILQTLVKQRKESIEQFTKGNRPELADKETKELAILESYLPASASKEEMNAA
                     IDKAIAETAANSMKQMGAVVKAAKSALEGKSVDGKALSDLVRERLAKLG"
     gene            complement(1804..2664)
                     /locus_tag="VII37_06595"
     CDS             complement(1804..2664)
                     /locus_tag="VII37_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069435.1"
                     /translation="MILAIKLPEGVSDPSLVAFLNDTKLGKLLIFDPTDEMTPFGQLR
                     GALQANYGLLVTSSGGQLVELPQLPATLNGTERIAKLKLDNKGTLSGDVREIRRGDPA
                     KHQRYTLQGVTQERDRIKPLEAALGDSLGTFQITKASIGNLAQTNLPFVYDYSVVAEN
                     YAKSAGDLLLVRPRVIGYESQNFMETKEARKYPVEFAGPWRNTDTFEIALPAGYEVDD
                     LPPPVNADYSFASYHSKAEVTGKTLKYTRSFEIKELSVPVSKADELKTFYRVIGNDER
                     NTAVLKPAAN"
     gene            complement(2661..>2890)
                     /locus_tag="VII37_06600"
     CDS             complement(2661..>2890)
                     /locus_tag="VII37_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069436.1"
                     /translation="QDLRPYVLQQEWLFQRPVPTVEARYTLQLPPGWEYKAVWVNHAE
                     IAPASVGNNQWQWVVKNVPAVKIHKSESLIT"
BASE COUNT          655 a          857 c          719 g          659 t
ORIGIN      
        1 ttttcatcca acaccgcgtt caatacgccg gccaatacgg cgttgcgcag caagtgtgtg
       61 cctacttcag aaacttctcc ggcataacga atcctaattc tcgacggacg cgtatccgaa
      121 gcttgcgaca ccagcgagcc caaccgttcc gcgagctcca tgtacgggcg cacccgccga
      181 tactgctcag cggaaagcac cggcagattc accgcgttcc tgataattcc ttccgccagg
      241 tagtctttca cctgcaccgc gacctgcgtt cccacttctt cctgagcctc cgtcgtcgac
      301 cctgcgatgt gcggagtgcc aacaacattg ggcaatccga ccagcggcga attttttggc
      361 ggctccacgg caaaaacgtc cagccccgcg ccgccgatgt gcccgctctt caaagcgtcg
      421 gccagagcgg cttcttcgat caattcaccg cgcgcggcat taatgatgaa cacgcctttt
      481 ttcatcttcg ccagcgattc cgcgttgatc agattttgtg tcgcgggact cagcgccgtg
      541 tgcagcgaaa caaaatcgct ctccgccagc aagcgctcca gcggcaccag ctccacggaa
      601 aattccttgg ccgccgcttc gctgatgtac gggtcataac ccagcacgcg catgtcaaac
      661 gcttcggcgc gcagggcaac ttcgctgccg atgcgcccca gcccgataag tcccagcgtt
      721 ttgccgctca attccattcc agccgcgctc gatttctccc agcgcccttc atgaaaggct
      781 ttatccaatc gcggaacctg ccgcgccagc gaaagcatca gcgcaaaagt gtgctccgcg
      841 acgcttaccg catttccgcc cggcgtgctc ataacgatga tgccgcgttt ggtagcggct
      901 tccagatcga tattgtccac gcccacgccg gcgcgcccca cgcaccgcag gttcggcgct
      961 ttatccagca ggtcggaact aacctttgta gcgctgcgca caatgagcgc ctgggcatcc
     1021 gcaatttccg caacgaggtt gtcttttgtg gtcaagacca cattccagcc aggctgctcc
     1081 ttgagcagct gcacaccccg cgcgctaatt ttgtcggcaa caattacttt catgcgcttc
     1141 ttctccgcct atgtggtgaa actttgcgtt tcgtgaacca tcaacggtag cgagccctca
     1201 ccgcagtgtc aatgcaatgg gcgaacaatt ctgtgataat tttgcaatta caaatatctt
     1261 gcgcgaaagc gcccagcgct tgcgatccaa attgaggagc cattgatgag tctgtctgag
     1321 cggattcaaa aggatttggt ggacgccatg cgcgccaagg atgagctacg cctgagcgtg
     1381 ctgcgcggca tgaaagccgc catcaagcat aaagaagtag aaaaaattcg cgtactcgac
     1441 gaaaccgaat ctattcagat cctgcaaacc ctggtcaagc agcgcaaaga atccatcgag
     1501 caatttacca aaggaaatcg cccggaactc gccgataaag aaaccaaaga actggccatc
     1561 cttgaaagct atctccctgc cagcgccagc aaagaggaaa tgaacgccgc catcgacaaa
     1621 gccatcgccg aaaccgccgc caattccatg aagcaaatgg gcgcagtagt caaggccgca
     1681 aaatccgcac tggaaggcaa atccgtcgat ggcaaagccc tcagcgacct ggttcgcgag
     1741 cgcctcgcaa aattaggatg agtggcaact cgcggaacgc cagcgctcga aacggtcgct
     1801 gacttaattt gcggcgggct ttagaaccgc ggtattacgc tcgtcatttc cgattacgcg
     1861 atagaacgtt ttcaactcgt cagctttgct gaccggcacg ctcaattctt tgatttcaaa
     1921 gctgcgcgtg tattttaatg ttttaccggt gacttccgct ttgctgtgat aactcgcgaa
     1981 actgtaatcc gcattaaccg gtggaggcag gtcgtctact tcgtatcccg ccgggagcgc
     2041 gatctcaaag gtatcggtgt tgcgccaggg tcctgcaaat tcaacgggat attttcgcgc
     2101 ttccttggtc tccataaaat tttgcgactc gtacccgatc actcgcggac gcaccagcaa
     2161 taaatccccc gctgatttgg cataattttc cgccaccacc gagtagtcat agacgaaggg
     2221 caaattcgtt tgtgccagat ttcctatgga agctttggtg atttgaaaag tgcccagcga
     2281 atcgcccagt gccgcttcaa gaggtttgat tctgtcgcgc tcttgagtaa caccttgcag
     2341 ggtgtagcgt tggtgtttcg caggatcgcc gcggcgaatt tcccggacgt ctccgctcaa
     2401 cgtgccctta ttatctaatt tgagtttggc gatccgctcc gtgccgttca gcgttgccgg
     2461 caattgcggc aattccacca gttgtcctcc ggaggacgtc accagtaatc cgtagttggc
     2521 ttgcagtgcg ccacgcaatt ggccgaacgg agtcatctca tcggtaggat cgaaaatcaa
     2581 taatttgcca agtttggtgt cgttcaggaa agctaccagc gaaggatcgg aaactccctc
     2641 cggtaattta atagccagaa tcatgtgatt aaagattccg atttgtggat cttgaccgcg
     2701 ggaacatttt tgaccaccca ttgccactgg ttatttccta ccgaggctgg cgcaatttct
     2761 gcatggttga cccacactgc tttgtattcc caacccggcg gaagttgcaa agtgtatcgc
     2821 gcttccacgg taggaacggg gcgctggaac aaccactcct gctgcaaaac gtagggtcgc
     2881 aaatcttgtt
//
LOCUS       DATCBB010000599         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_214234, whole genome shotgun sequence.
ACCESSION   DATCBB010000599 DATCBB010000000
VERSION     DATCBB010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214234"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VII37_06605"
     CDS             complement(<1..1604)
                     /locus_tag="VII37_06605"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY5069437.1"
                     /translation="MRSVRGFLLSIFTILVIAAGSGIWWFVYRPLPQVDGTVVVNGLH
                     RDVSVERDRWSIPHIRASSAEDMVEAQGYVMAQDRLWQMDLLRRAARGQLSEIFGPAV
                     LSIDKRFRTMGFGHAAEREAAAAKGEPRLIVEAYARGVNRFIEQHQNQLPLEFSLLNY
                     KPQPWTPADTFVIGGYMYQTLTDTWESKLDRANVIERVGLDRAKDLFSSEAAMDHFIV
                     GDPNDKKDGSQHAHSGDDDEDEDEMDSDDVIKAQNLKPHGPREEEIPPDINSMLWPAA
                     RDFLSGSNRGMRKSLGSNNWVVSGAHTATGKPLLANDTHLELSIPSIWYQVHLTAPGW
                     NVEGFTFPGAPLVVIGHNDRIAWGFTNNGADVQDLYIETFNPATPDEYRVNGAWKKAQ
                     TLDEVIHIKGQADQHLAVVSTRHGPIVRRDGNKGYALRWTATEPGALSNSYNWLGAAQ
                     NWDEFRKAIKQSWGPAQNAVYADVDGNIGYVMSARVPIRKKGHGEVPVPGDTDDYEWT
                     GYIPFEQLPQALNPESGLIVTANARVT"
     gene            1721..>2235
                     /locus_tag="VII37_06610"
     CDS             1721..>2235
                     /locus_tag="VII37_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY5069438.1"
                     /translation="MFVLRLIGVLALIVLNGFFAAAEFSLVAVRISRVRQLVAKGNLR
                     ARVVEMLVGDLRRVVSGVQLGITLSSLAIGALGESSLAKLFQQIWPGTLGTHSALIAH
                     AVALTVAFALLSALHVVIGELVPKTISLSRAERVALLIALPFHWFLKTFRWAIYLLEA
                     TSNGIIKALGVT"
BASE COUNT          429 a          592 c          640 g          574 t
ORIGIN      
        1 gtgacccgtg cattggcggt aacaatcaag ccactttctg gattgagcgc ctgaggcaat
       61 tgttcgaatg gaatgtatcc cgtccattcg tagtcgtcgg tatcgcctgg caccggcact
      121 tctccgtggc cttttttgcg aatgggaacg cgcgcagaca ttacgtaacc aatatttccg
      181 tcgacatcgg catatacagc gttttgcgcg gggccccacg actgtttgat cgctttgcga
      241 aattcatccc agttttgtgc cgcgcccagc caattgtagg aatttgagag agcacctggc
      301 tctgtagccg tccagcgcag ggcgtatcct ttgttgccgt cgcgtcgaac tattgggccg
      361 tggcgagttg atacgaccgc gaggtgctgg tcagcttgcc cttttatatg gatgacttcg
      421 tcgagtgttt gtgctttttt ccaagcgcca tttacgcggt attcatccgg tgtagctggg
      481 ttgaacgtct cgatgtataa atcctggacg tccgcaccgt tgttggtgaa gccccaggcg
      541 atgcgatcgt tgtgaccgat gaccaccagt ggcgcacctg gaaatgtaaa gccttcaaca
      601 ttccaaccag gcgcggtgag atgaacctgg taccagatgg agggaatgct taactccaga
      661 tgcgtatcgt tggctagcag cggttttccg gtagcggtgt gcgcgccact aacgacccag
      721 ttgttacttc ccagtgactt tcgcattccg cggtttgagc cggaaagaaa atcacgcgct
      781 gcgggccaca acatcgaatt tatgtccggc gggatttcct cttcccgggg gccatgaggt
      841 ttaagatttt gcgccttgat gacgtcatct gaatccattt cgtcctcatc ttcatcatca
      901 tcgccgctgt gagcgtgctg ggagccgtct tttttgtcgt tcgggtcgcc aacgatgaag
      961 tggtccatcg cggcttccga agaaaaaaga tctttggcac gatctagtcc gacacgctca
     1021 attacattgg cgcggtctag tttgctttcc cacgtatccg tcagggtttg gtacatatag
     1081 ccgccaatga caaaggtgtc ggcaggcgtc cacggttggg gtttgtaatt cagaagagaa
     1141 aattccagcg gcagctgatt ttggtgctgc tcgataaatc tgtttacccc tcgagcataa
     1201 gcttccacga ttaggcgagg ttcaccttta gccgctgcgg cctcgcgctc ggcggcatgc
     1261 ccgaaaccca tagtgcgaaa tcttttgtcg attgacagta ccgccggacc aaatatttcg
     1321 gagagttgcc cgcgggcagc gcggcgcagc agatccatct gccaaaggcg atcctgggcc
     1381 attacgtagc cctgcgcttc caccatatct tccgcggagc tcgcgcggat gtgcggtatg
     1441 ctccagcggt cgcgctcgac ggacacatcg cggtgcaagc catttacgac tacagttccg
     1501 tccacctgtg gcagcgggcg atagacaaac caccagattc cgcttcccgc ggcaatcacg
     1561 agaattgtga atatcgaaag taagaatcct cgaacgctac gcaagcgctt ccttagtcat
     1621 ctggagtttc caatcgagtg tagaagacgg cgtaccgtca agcaacggcc aacgcctggc
     1681 aattgtctgc tgctacaatg cggcagggga ctgtgtgtct atgtttgtgc tgcgcttgat
     1741 tggcgtgttg gcgctgattg tcctgaacgg attttttgcc gccgcggagt tttcgcttgt
     1801 ggcggtcaga atatcccggg tgcggcaatt ggttgccaag ggcaatctgc gggcgcgcgt
     1861 agtggagatg ctggtgggcg atctgcgccg tgtggtttcc ggcgtgcagc tggggattac
     1921 gctttccagt ttggcgattg gcgcccttgg cgaatccagc ctggcgaaat tgtttcagca
     1981 gatctggcca ggcactttgg ggacgcactc tgctctaatt gcgcacgcgg ttgcgctgac
     2041 ggtggcgttt gcgttgctca gtgcgctgca cgtggtgatc ggggaactgg ttcccaagac
     2101 gatcagcctt tcacgcgccg aacgcgtggc gttgttgatc gccttaccgt ttcactggtt
     2161 tctaaaaact tttcgctggg cgatttatct tttggaggct acttccaacg ggattatcaa
     2221 agccctgggc gttac
//
LOCUS       DATCBB010000600         1088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_474276, whole genome shotgun sequence.
ACCESSION   DATCBB010000600 DATCBB010000000
VERSION     DATCBB010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474276"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1088)
                     /locus_tag="VII37_06615"
     CDS             complement(<1..>1088)
                     /locus_tag="VII37_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5069439.1"
                     /translation="ESEAAFAALVERHVNLVYSTALRSVGGTHAAEEITQAVFIILAR
                     KAGKMLRRTVLSGWLYQTTRLTAANFLRTEIRRQNREQEAFMQSTLSETETWTQIAPL
                     LDDAISRLGERDRNAILLRYFENKSAREMAAALRVDEPAAQKRLTRAVEKLRKFFAKR
                     GVTLTAAAIAGAVSANSVQAAPIGLAATVTAAAAKGAAVSASTLTLIKGALKIMAWTK
                     MKTAIVVGVTAILAAGTTTVVVKEINSRQVYYKGKSLQVWLKNLDDQHPGPANDEATD
                     AVRHIGKNGLPIIISLLQLKNPLHHSAVLACQILGSDAKPAIPALMKLLDSGYANGYV
                     GAAFDEIGSDAIAPLIETLTNENAEVRT"
BASE COUNT          224 a          306 c          281 g          277 t
ORIGIN      
        1 ggttcgcact tctgcgtttt catttgtgag cgtctcaatt aatggagcaa tggcatccga
       61 tccaatttca tcgaaagcag ccccaacata tccgtttgca taaccactat cgagtaactt
      121 catcaaagct ggaattgcag gtttggcatc tgaaccaagt atttgacaag caaggacggc
      181 actatggtgt aatggatttt tcaattgcaa caaactgata attattggaa ggccattttt
      241 tccgatgtga cgaaccgcat cggtagcttc atcgtttgcc ggaccaggat gttggtcatc
      301 caaatttttc agccatactt gtagcgactt gcctttgtaa tagacttgtc tgctgtttat
      361 ttctttcacc acaaccgttg tcgttcctgc ggccaaaatt gcggtcacac caacaacaat
      421 cgccgttttc attttagtcc acgccataat tttcaatgct cctttgatga gggttaaagt
      481 tgaggcgctg accgccgcgc cttttgccgc cgccgccgtg accgtcgccg ccagaccgat
      541 tggcgcggcc tggacggaat ttgccgaaac tgcgccagca atcgccgccg ccgtcagcgt
      601 gacgccgcgc ttcgcaaaaa atttccgcag tttttccacc gcgcgcgtca agcgcttttg
      661 cgccgccggt tcgtccacgc gcagcgccgc cgccatttcg cgcgcagatt tgttttcaaa
      721 ataccggagc aggatggcgt tgcggtcgcg ctcgccgagt ctggaaatgg cgtcgtcgag
      781 cagcggcgcg atttgcgtcc acgtttccgt ttcactcaaa gttgattgca taaatgcctc
      841 ctgttcgcgg ttctggcggc ggatttccgt ccgcagaaaa ttcgccgccg tcagccgcgt
      901 cgtttgataa agccagccgg acagcaccgt gcgccggagc atttttcccg ccttgcgcgc
      961 gaggatgatg aagaccgcct gtgtgatttc ctcggcggcg tgcgtgccgc caacgctgcg
     1021 taatgcagtg gaatagacaa gattgacgtg ccgttcaacg agcgcagcaa aggcggcttc
     1081 ggattccg
//
LOCUS       DATCBB010000601         1100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_311535, whole genome shotgun sequence.
ACCESSION   DATCBB010000601 DATCBB010000000
VERSION     DATCBB010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311535"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          234 a          319 c          281 g          266 t
ORIGIN      
        1 atggcgcttg gtatgcatat ggtgtcggcc cgtgctgggt caacgttgac ggcctgtggt
       61 tctggaacgc tgcggtttgc gctccctgaa agcttggatt gcagctatcg caatctgatc
      121 gattgcttct aagatcaaat aaaaccccgg gcaaaatgtc ccggggtttt tcgttgacag
      181 atattgggtg ctcacatcat gccgtgtttc gtccccatgt ctccgtttaa ggcggtggtc
      241 tcaaccgatc gacgcaacac cgggggccag ctggcaataa ataacgatga cggcttgcca
      301 aacgctcacc aaatcgcttt cggcgacttc cctcgcaacc agctatccca caacactatt
      361 ttctctgccg cccccatacc actacgaagt cactgaccta tcgtcctaca ctctctatct
      421 ttttcctcta cgataaagtg ttgcgtcgat cggttgagcc ggcttcaaaa gcggacattg
      481 gccggcgccc gaacggcgat ttcggcgcat cagcaactga atgtattttt ctctccagaa
      541 taattcattt cgttgcttgg cgcccgccag gcaatcactt cttgaaaccg gcatcacgcc
      601 cactgttgtc acaacgaatt aatttggctc gcaatcgaca aaattatttc attagtacgc
      661 cgttcgcagc gcagcgcaat cgcaacgaat tcaagcttga cttgattcca gatcttggcg
      721 gcgcgcggcc gtgatcactt tgggctccac gatcagcaaa tcatgcgcga cctcaagctg
      781 ccgatgttcg agtccagcgc gcaactggcg tggccgccgc atcggccgat cgattggtcg
      841 agcgcggttc ccgtggcgcg ccacccagtc gcgaactggg cgagcgagcg ccaggaacgc
      901 gcgcaggccg tgcgggagga gcatgagcgg gttacgatcc gcgattattc agcgccgcaa
      961 gaccggcttg tagctcgtcg aattttttct gcaatgatgg attgccttct tgccactcac
     1021 gcacgagccg gttgatggct tcaatacatc gctccagatg aaaaagctgg agtctacctt
     1081 gtgcaatatc acgttctgaa
//
LOCUS       DATCBB010000602         1292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_117884, whole genome shotgun sequence.
ACCESSION   DATCBB010000602 DATCBB010000000
VERSION     DATCBB010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117884"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VII37_06620"
     CDS             complement(<1..992)
                     /locus_tag="VII37_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HEY5069440.1"
                     /translation="MADLKQLARRIFHETLAAIDIPLTIQRKLAREGSLLHCGKATID
                     LSKFERVRVVAIGKAAHAMLDGLKACLPPDFSFAGIASAPVAPANPHRGIQYFLGGHP
                     IPNEDSWNAAKAVLEMLGACDSQTLVFFLLSGGGSALIELPLDPQLSLADMQQIHRLL
                     IGCGAPIEAINTVRKHISAVKGGRLATAANRATKITLAVSDVPAGKEWALASGPTLPD
                     PTTRSDVEKILNEYALRDKFPEEFHRWVARESIPETPKADDPAFATAHFSLLLGLDNL
                     FHPAHGAAEAAGCVTCCDNVTDDWPVERAAEYLLAQLEELRSNNPTRRVAVIADG"
     gene            complement(1006..>1292)
                     /locus_tag="VII37_06625"
     CDS             complement(1006..>1292)
                     /locus_tag="VII37_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5069441.1"
                     /translation="FINTARGSVVDEAALVHALESKKIAGAALDVYEKEPFILAGLKR
                     PNVVLAPHLASASLETRTKMACIAATNVRDFFDGKRPANILNPEVLKTAK"
BASE COUNT          286 a          357 c          351 g          298 t
ORIGIN      
        1 ccatcagcga ttaccgctac acgccgcgtg ggattattgc tgcgcaattc ttcgagttgc
       61 gccaatagat attccgcggc gcgctcaacg ggccagtcgt cggtgacgtt atcgcaacag
      121 gttacgcagc cggcggcttc cgccgcgcca tgcgctggat gaaacagatt gtccagcccc
      181 aacaaaagtg aaaaatgcgc agtggcaaac gcgggatcat cggctttcgg agtttctggt
      241 atggattctc gcgcaaccca gcgatgaaac tcctccggaa atttatctct caacgcgtat
      301 tcgttcagaa ttttttccac gtcagaccgc gtggttgggt cgggcaatgt gggcccgctg
      361 gccagcgccc attcttttcc ggcgggaacg tcgctgactg ccagtgtaat tttggtggca
      421 cgattcgccg cagtggctaa ccgtccacct tttaccgcgg aaatatgctt gcgcaccgtg
      481 ttaatggctt ctatgggagc gccgcaaccg atcagcagtc ggtgtatctg ctgcatgtcc
      541 gccaaactaa gttgcgggtc gagaggtaat tcaatgaggg ctgagcctcc gcctgacagc
      601 agaaaaaaaa ccagcgtctg actgtcgcaa gcccccaaca tttccaggac tgctttcgcg
      661 gcgttccaac tgtcttcatt gggaatcgga tgtccgccca ggaaatattg aatgccgcga
      721 tgcgggtttg ccggggcaac cggggctgaa gcaatacccg cgaatgaaaa atctggcggc
      781 aaacaagctt tgaggccatc caacatcgcg tgagcggctt ttccgatggc cactacgcgt
      841 accctttcaa atttggatag atcgatggtt gccttgccgc aatgcagcaa cgatccttca
      901 cgcgctaatt ttcgttgtat ggtcagcgga atgtcgatgg ccgccaaagt ttcgtggaag
      961 atacggcggg cgagctgctt caaatccgcc atgggaatta gcggctcact tagcagtttt
     1021 caaaacttcg ggattcaaaa tattcgcagg gcgttttcca tcaaaaaaat cgcgcacgtt
     1081 ggttgctgca atgcaggcca tcttggtgcg cgtttccaac gaagcggacg ccaggtgcgg
     1141 agcaagcaca acattcgggc gtttcagtcc ggccaggata aacggctctt tttcgtaaac
     1201 gtctaaagcc gcgcctgcaa tcttcttgct ttcaagcgcg tgcaccagcg ccgcttcgtc
     1261 caccactgag ccgcgcgctg tattgatgaa aa
//
LOCUS       DATCBB010000603         1194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_343957, whole genome shotgun sequence.
ACCESSION   DATCBB010000603 DATCBB010000000
VERSION     DATCBB010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343957"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VII37_06630"
     CDS             complement(<1..795)
                     /locus_tag="VII37_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY5069442.1"
                     /translation="MIPRYTREEMGAVWSDSNKYAKWLEVELAATETLAEAGDVPKDA
                     AARIRAHAKINVARIQELESRVKHDVVAFTMAVGESIGDASAARWFHYGMTSNDVVDT
                     AQALQVRDASRLIEAALVKFGDALAARAQEFRATPQIGRTHGVHAEPITFGLKVANWY
                     AENQRNQQRFRDAAAQMAVGKVSGAVGNASHLGPDIEERICQRLGLTVAPVASQVIQR
                     DRHAQYLSALAILTATLEKIALEIRHLQRTEVREAEEPFGGEQRGSS"
     gene            complement(906..>1194)
                     /locus_tag="VII37_06635"
     CDS             complement(906..>1194)
                     /locus_tag="VII37_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069443.1"
                     /translation="TGQRESFVLVLDEVQALVLNDVKAGNIILDLVIRKGAEITKSDI
                     EQVYGMIDDSVTNNKLQTAVNGGFQILEINPSYGAEGLILFKTWSLVPQTD"
BASE COUNT          225 a          351 c          342 g          276 t
ORIGIN      
        1 ggagctgccg cgctgctcgc cgccgaacgg ctcctcggcc tcgcggactt cggtgcgctg
       61 caggtgccgg atttcgagcg cgattttttc cagcgtggcc gtaaggatcg ccagggcgga
      121 aagatactgt gcgtggcggt cgcgctgaat cacctgcgaa gcgacgggcg cgacggttaa
      181 ccccagccgc tggcagatgc gctcttcgat atccgggccg aggtgcgaag cgttgcccac
      241 cgcgccagaa actttgccga ccgccatttg cgcggcggca tcgcggaagc gctgctggtt
      301 gcgctgattt tcggcgtacc agttggcgac tttcagtcca aaagtgatgg gctcggcgtg
      361 gacgccatgc gtgcggccaa tttgcggagt ggcacgaaat tcctgggcgc gcgcggccag
      421 cgcgtcgcca aatttcacca gtgcggcttc aatcaatcgc gacgcgtcgc gcacctgcag
      481 ggcctgcgcg gtgtcgacca catcgttgga ggtcatgccg tagtgaaacc agcgcgcggc
      541 ggatgcgtcg ccgatggatt cgccgaccgc catggtgaag gccaccacgt cgtgcttcac
      601 gcgcgattcc agttcctgga tgcgcgcgac attgattttg gcgtgggcgc gaattcttgc
      661 ggcggcgtct ttcggcacgt cgccggcttc cgccagtgtt tccgtggcgg ccagctccac
      721 ttccagccat ttggcgtatt tgttggagtc gctccagaca gcgcccattt cttcgcgggt
      781 gtagcgtgga atcaactttg gcctctattc aaaatagatc agacgaaaaa ctcgatgcgc
      841 gaatctatag taataatacg gtatttccga ggtgcctaca cgtcgcgctt ggttcgcatg
      901 ccgcgttagt cagtttgggg tacgagactc cacgttttga ataaaatcaa accctctgca
      961 ccatacgacg gatttatctc cagtatttga aacccaccgt tcaccgctgt ctgtaattta
     1021 ttgttcgtta cagaatcatc gatcatgccg taaacctgtt ctatatcgct cttggttatt
     1081 tccgcgccct tacgaatgac caagtccaaa ataatgttgc cggcctttac atcgtttaga
     1141 accagggcct gcacttcatc caacaccaag acgaaagact ctcgctggcc tgtc
//
LOCUS       DATCBB010000604         1160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_376344, whole genome shotgun sequence.
ACCESSION   DATCBB010000604 DATCBB010000000
VERSION     DATCBB010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376344"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..949
                     /locus_tag="VII37_06640"
     CDS             62..949
                     /locus_tag="VII37_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5069444.1"
                     /translation="MTQRDLVTSRRRGLFGRRVPTQMPGGESTALVKSRPARNPVSQL
                     TRTQEYFLEWNAQRLGITFAESHARYIQSWDAVPHGHNGRAFEKFHGQCYELFRVFAD
                     DRPTEVIDAYKLHAVVHFLTMLTYPEPQWFDEDVIVKQFRNRSAISILDFGCGLAQHS
                     RTLAEYMLAKGLKVNIALADIPTVREDFLIWWGKRCGIPTTFLECSVEHPIPELPQVD
                     LCFALEFFEHVYDPVTYFIRINEAMTPGGVLVTNFSDHHKEFMHVSPQLAPLREAVQE
                     RGYEEIFANFIYRKPHAAP"
BASE COUNT          241 a          350 c          324 g          245 t
ORIGIN      
        1 cagcgactcg tagcaatcgt cggcacactt gcgagctgcc attgaatcaa ggattctcat
       61 catgacgcaa cgggacctgg tgacctcccg cagacgcggc ttgttcggtc ggcgggtgcc
      121 gacacagatg cctggcgggg agtccacggc gctcgtgaag tcgaggcccg cgcgcaatcc
      181 cgtctcgcag ctcacgcgca cgcaggagta tttcctggaa tggaatgcgc agcgccttgg
      241 aataacgttc gccgaaagcc acgcgcgcta catccagtca tgggacgccg tgccccacgg
      301 tcacaatggc cgcgcattcg agaaattcca cggccagtgc tacgagctgt ttcgggtgtt
      361 tgccgacgac cggccaactg aagtcatcga cgcttataaa ttacacgcgg tggtgcactt
      421 cctgacgatg ttaacgtacc ccgagccgca atggttcgat gaggatgtca tcgttaagca
      481 gttccgcaat agatcggcaa tcagcattct cgattttggt tgtggactcg cgcagcattc
      541 cagaacactg gcggaatata tgctggccaa gggactcaaa gtgaatatag cgcttgccga
      601 catcccgacg gtgcgcgagg attttctgat ctggtggggc aagcgctgcg ggattcccac
      661 gacgttcctc gagtgcagcg tcgaacatcc cattcccgaa ctgccgcaag ttgatctgtg
      721 ctttgctctg gagtttttcg agcacgtcta cgatccggtc acgtatttca tccgcatcaa
      781 tgaggcaatg acgcccggtg gcgtactggt cacaaatttc tccgatcatc acaaagaatt
      841 catgcatgtc tccccacagc tcgcgccact gcgcgaagcc gtgcaggaac gcggctatga
      901 ggagatcttt gcgaatttca tttaccgtaa accgcatgct gcgccgtgat cgcgcagctg
      961 cggctcagta gtttgcagcg gcggcgttgc cgagtcttcc ctcgccatca gcgatgacca
     1021 atactcgccc tcccccaaga gtcgcacggg aagcgctcaa atggcagcgc taaaggccgg
     1081 cacgcccgca ccagcattct cgttgtcgag tacacctgag cagcgcgtcg cactgtctca
     1141 gtttcgcggt cgccccgtga
//
LOCUS       DATCBB010000605         1416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_279232, whole genome shotgun sequence.
ACCESSION   DATCBB010000605 DATCBB010000000
VERSION     DATCBB010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279232"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1416
                     /locus_tag="VII37_06645"
     CDS             <1..>1416
                     /locus_tag="VII37_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5069445.1"
                     /translation="IESNPTNLDPRFASDGQSQHLDGLIFSSLVERDEQMNLHGDLAE
                     SWDTPDPLTYIFHLRPGVRFHDGSAVTAKDVKATFDFILNAANHSAKRGAFRMIASIE
                     APDARTVIFHLKEPYASFLWTVSRPAIGIVPADASANLSRGLIGSGPFRFVSQAQDDE
                     VVLAANTNYFGGAPDISRLRARVVPDAIVRALELRKGSADLEISSLSPDMVPVLARRP
                     ELGVSERAGTNLAYLGFNMDNAIVGRREVRQALAYATDREMLIRYLLHGQARIANGVM
                     PPNHWAYESNVTTYSLDTARAEALLDAAGFPRKENGVRMHVMLKTSTDEQARLVGAAL
                     QEQWRHVGVALEVRSLEAATLLSDLVKGNFEINLFRWVGANNDPDVFEFLFSSKRFPP
                     DGANRGHYRNARMDALTDQIRVEMDREKRRALCSEAQKILATDLPYLPLWFVDAVSVH
                     QKELGDLPLSPSGDFDFLAKL"
BASE COUNT          277 a          318 c          477 g          344 t
ORIGIN      
        1 gattgaatca aatccgacta atcttgatcc gcggtttgcg agtgacgggc agtcgcagca
       61 tctggatggt ttgatattca gcagcctggt ggagcgcgat gagcagatga atttgcacgg
      121 ggatttggcg gagtcgtggg atacgccaga tccgctgact tatatttttc atttgcgacc
      181 cggagtgcgc ttccacgacg gcagcgcggt caccgccaag gatgtcaaag ctacatttga
      241 ttttattttg aatgctgcga accactctgc caagcgcggg gcgtttcgca tgattgcatc
      301 catagaggcg ccggatgcgc gtacggtgat ttttcatttg aaggagccgt acgcgtcgtt
      361 tttgtggacg gtatcgcgtc cagcaatcgg gattgtgccg gcagatgcaa gcgcgaactt
      421 gtcgcgtgga ttgattgggt cggggccgtt tcgattcgtg agccaggctc aggatgacga
      481 ggtggtgttg gcggcgaata caaattattt tggcggcgcg cctgatattt cacgattgcg
      541 ggcgcgcgtg gtacccgacg cgattgtgcg ggcgttggaa ctacgcaaag gctcggcgga
      601 tttggagatt agttcccttt caccggacat ggtgccagtg ttggcgcggc ggccggagct
      661 cggcgtatcc gaacgtgctg gaacgaacct ggcgtatctc ggcttcaaca tggacaacgc
      721 gattgtcgga cgacgggaag tgcggcaggc gttggcatac gcgacggatc gcgagatgct
      781 gattcgttat ttgctgcacg ggcaggcgcg catcgcgaat ggagttatgc cgccgaacca
      841 ttgggcgtat gagtcgaacg tgacgaccta ttccctggat acggcgcgtg cggaggcgtt
      901 gctcgacgcg gctggattcc ctcgcaaaga aaatggcgtg cgaatgcatg tgatgctgaa
      961 gacttctacc gacgagcagg cgcgactggt tggggcggcg ctgcaggaac aatggcggca
     1021 cgtgggtgtt gcgttggaag tgcgttcgct ggaggcagcc acgctgcttt cggatctggt
     1081 gaaggggaat tttgagatca atttgtttcg ctgggtggga gcgaataacg atccagacgt
     1141 ttttgaattt ttgttttcgt cgaagcggtt ccctccggac ggggcaaatc gtggtcacta
     1201 ccgaaatgcg cggatggacg cgctgaccga tcagattcgc gtggaaatgg atcgcgagaa
     1261 gcgcagggcg ctgtgtagcg aagctcagaa gattttggct acggatttgc cgtatttgcc
     1321 gctgtggttt gtggatgcgg tttcggtgca tcagaaggaa ttgggagatt tgccgctgtc
     1381 gcccagcgga gattttgatt ttttggccaa attgaa
//
LOCUS       DATCBB010000606         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_409716, whole genome shotgun sequence.
ACCESSION   DATCBB010000606 DATCBB010000000
VERSION     DATCBB010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409716"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1871)
                     /locus_tag="VII37_06650"
     CDS             complement(15..1871)
                     /locus_tag="VII37_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069446.1"
                     /translation="MQLRLRTKLTIVMTGLVLLVVAVLSGVFLAQLLGQTLLETDKRA
                     SDLAHQVFVQAQHALADAANQGLRPSSDDPDDIHDYVRRAFEINAGLQAQLEAAISSP
                     SVYEVSITDDEGLVLISNDQSLPGKTQPRRPPLSQLVQSGFVNQVKVLRGPSRIFEYD
                     YPFNMASKPFGEVRVVLSTGLLLNEIAPSLKTSGTIVLLALGICTLLAAVVSGATLAP
                     LRDISAQLDRISAGQYDTPSGSLRIFGGSGDELGQVSRKITQVGQQLRGVHEIFSTLR
                     ENMNSVMAGLEDGLLLFTRDSRAVMVSPAAEKFLGAPSGEFLGRRVEDIFPEGHPLRA
                     VLHLEGDELSEVAAETELVTAGVARRVGVSVQAIQEGGERIGALVTLRDLDSLESINT
                     QLQVSERLAALGRITAGVAHEVKNPLNSMRLWLENLKESLPADQESGAQQAVNVLDKE
                     IDRLDAVVKRFLDFTRPMDIRLEATQLSQLLPEVLEIAQPQLQKAGIQLAQLLPIDVP
                     EVYVDRALLKQAVLNLVLNAAEAMPNGGQLSLVLSRRGEMAEITVQDTGKGIPPENKQ
                     KIFQLFFTTRPGGSGIGLASAFRIVQLHNGSIDFTSEVGRGTTFRIELPLAA"
     gene            complement(1880..>2521)
                     /locus_tag="VII37_06655"
     CDS             complement(1880..>2521)
                     /locus_tag="VII37_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY5069447.1"
                     /translation="EMPAPTQAKLLRVLEDRKVRRLGSKSETPVDVRVLAATNKDAEQ
                     AVASGDLRQDLFFRLNVFHINLPPLREHKEDIPLLVEHILRDVNSKHGKHVRGIGAEV
                     LDIFMSHTWPGNIRELRNILERACIMCEKELITRSCLPEEFGRTAAKGPSDLSSIKFP
                     VGTTVDAMERELILQTLSATGNNKTRAADLLAISLKTLHNKLKEYGADRAGAE"
BASE COUNT          549 a          716 c          675 g          581 t
ORIGIN      
        1 ttcgccgtca ttagttaggc cgctagaggc aactctatgc ggaaagtcgt gccacgacct
       61 acttctgaag taaagtctat cgaaccgttg tgaagttgca caattctgaa tgcgctcgcc
      121 aggccgatac cacttccacc cggccgcgtg gtgaaaaata attggaaaat tttctgctta
      181 ttttcgggtg gtatgccctt gccggtatct tgcacggtga tttccgccat ttcgcccctc
      241 cggctaagaa ccaggctcaa ttggccgcca ttaggcatcg cttccgccgc gttgagtacg
      301 agattcagca ccgcttgttt cagtaaagca cggtctacat atacttcggg aacgtcaatc
      361 ggcagcagtt gtgccagttg aatacctgcc ttctgcaatt ggggctgagc gatttcgagg
      421 acttccggca gcaactgaga aagctgcgtg gcttccaatc gaatatccat cggccgcgtg
      481 aaatcgagga agcgcttgac caccgcgtca aggcggtcga tttccttgtc cagcacgttg
      541 accgcctgct gcgcgccact ttcctggtcc gctggtaaag actcttttag attttccagc
      601 cacagtcgca tggagttcag cggatttttt acttcatgcg caacgcccgc ggtgatgcgg
      661 ccgagcgcgg ccaggcgttc gctcacttgc agttgggtat tgatactttc cagagagtcc
      721 aaatcacgta aagtaacgag agctccgata cgctccccgc cttcttgaat cgcctgcaca
      781 ctgacgccaa cgcgccgagc cacgccggcc gtgaccagtt cagtttccgc cgcaacttcg
      841 ctgagttcat cgccttccaa atgcagcacc gcacgcagcg gatgcccttc agggaaaatg
      901 tcctcgacgc gacggcccag aaattctcca gagggcgcgc cgagaaattt ttccgccgcg
      961 ggactgacca ttacagcgcg cgaatcccgc gtgaataaca acaagccatc ttcgagaccg
     1021 gccatgacgg aattcatgtt ctcgcgcaag gtactgaaaa tttcatgcac gccccgaagt
     1081 tgctgtccga cttgagtgat ttttctgctt acttgcccga gctcgtcgcc acttccgcca
     1141 aaaatgcgca gacttcccga tggcgtgtca tattgcccgg cggaaattcg gtccagttgt
     1201 gctgagatgt ctcgcaacgg tgccagtgtg gctccgctaa cgaccgcagc caggagcgtg
     1261 cagatcccca gtgcgagcaa aacaatagtt ccagaagttt tcagactggg ggcaatttca
     1321 ttcagcaaca agccggtaga caacaccacc cgcacctcgc cgaatggttt gctggccata
     1381 ttgaaagggt aatcatattc aaaaattctc gatggtccgc gcaacacctt gacctggttc
     1441 acgaagccac tctgcaccaa ctgcgagagg ggagggcgtc gcggctgagt cttgcctggc
     1501 agggactggt cgttggaaat caataccagt ccctcgtcat ccgtaatcga aacttcatac
     1561 accgacggcg agctgatggc cgcttccagt tgcgcctgca agccggcgtt aatctcaaac
     1621 gcccggcgaa cataatcatg aatatcgtct ggatcgtcgg acgaagggcg aaggccctgg
     1681 ttggccgcgt ctgctaacgc gtgttgcgcc tgaacgaata cctgatgagc aagatcgctt
     1741 gcgcgcttat cggtttcaag cagggtttgc ccgagaagtt gcgccaaaaa tacgccggac
     1801 aacacggcga ccaccagcag caccagcccg gtcatgacga tggttaattt tgtgcgcagc
     1861 cgtagctgca tgaaagtccc tattccgcgc cagcgcggtc agcgccatat tctttgagct
     1921 tattgtgcaa agttttgagg ctgattgcga gaaggtctgc ggcgcgtgtc ttattgttac
     1981 cggtggcact gagggtttgc agaatgagct cgcgctccat ggcatccacg gtggtgccca
     2041 ccgggaactt gatgctggaa agatcgctgg gccctttggc ggcggtacgc ccgaattcct
     2101 cgggcagaca cgaacgggtg atcaattcct tctcgcacat aatgcaggcg cgttccagga
     2161 tattgcgcag ctcgcgaata ttgcctggcc aggtgtggct cataaaaata tcgagcactt
     2221 ccgctccgat gccccggaca tgtttgccgt gtttggaatt aacatcgcgc aagatgtgct
     2281 cgaccagtag aggaatgtct tctttgtgct cgcgaagcgg cggcaaatta atatggaaaa
     2341 cgttcaaacg gaaaaataaa tcctggcgca agtctccact ggccaccgct tgttcggcgt
     2401 ctttgttcgt agctgcaagc acccgcacgt cgaccggcgt ttcgcttttg ctcccgaggc
     2461 ggcgaacttt gcggtcttcc agtacacgca ataatttggc ttgtgtaggc gcgggcattt
     2521 c
//
LOCUS       DATCBB010000607         1573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_409770, whole genome shotgun sequence.
ACCESSION   DATCBB010000607 DATCBB010000000
VERSION     DATCBB010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409770"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VII37_06660"
     CDS             complement(<1..1556)
                     /locus_tag="VII37_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069448.1"
                     /translation="MPYLNPGQLVVLWSKIQGRHNVVSAGDFQDWQRENTTFEALVSS
                     SGASFNLARPGSQPEMVDGTRSTYGFFDKFFGERPWMGRYFLPDEMQAGKDHVVILTH
                     RMWEHMGSNPNIIGTQLRLDGEPYAVVGVRPPGAPDRMAGDIFAPMTFKPEQLNHDFH
                     WLLAFGRLKPGVSLAQAQADMNVVAHRIAQDNPKSNKGWDISVEPLHNDFLPPEAKKS
                     MWILMAAVGFILLIACVNVANLLLARSTTRQREVAVRAALGASRRQVFAQFLSESLVL
                     AFIGGVLGTALGLVMIKIFMATMPQYTLPSEADVRLSVPVLLFTISVTMISGVIFGCA
                     PAWQASKLDLNETLKEGGRSASGSARNGLRRTLVILEFALALTLLASAGIAVHSFWKL
                     ARVNPGFEADHLLTFSLPVPNKKFSAPEQMIAFYRQVLDKVESLPGVTRAEVGEGRPL
                     QGTEFGMAFNIAGKPVSDPSARPGAGFSMVTPGYFQTFGIQMARGRSFTEQDVASSIP
                     VAVVNETFADR"
BASE COUNT          352 a          469 c          436 g          316 t
ORIGIN      
        1 cgatccgcga aggtttcgtt cactacagcc acgggaatgc tgctggcgac gtcctgttca
       61 gtgaagcttc gcccgcgcgc catctgaatc ccaaatgttt ggaaatatcc cggagtgacc
      121 atcgaaaaac cagcacccgg ccgcgcggaa ggatcgctta cgggctttcc ggcaatgttg
      181 aatgccatgc cgaactcggt gccctgtaac ggcctccctt cgccgacctc ggcacgggtt
      241 acgcctggaa gcgattcaac tttgtccaga acctggcggt agaacgcgat catctgttct
      301 ggcgcagaga attttttgtt ggggaccgga agagaaaacg taagtaagtg gtcagcctcg
      361 aagccgggat tgacgcgtgc caatttccag aagctgtgga cggctatccc cgcgctggct
      421 agcagcgtga gcgccagagc aaattccaga attacgagcg tgcggcgcaa cccgttgcgg
      481 gcggagcccg aggccgagcg gcctccttct tttaacgtct cattgagatc gagtttcgat
      541 gcctgccatg cgggggcgca tccgaagatt acgcccgaga tcatcgttac cgagatggtg
      601 aagagcaata caggcacgct caagcggaca tccgcctctg acggcaaagt gtattgcggc
      661 atggtcgcca taaagatctt gatcatcacc aggcccagtg ccgttcccag cacgccgcca
      721 ataaacgcca acaccaaact ctcgctcagg aattgagcga acacctgacg ccgcgacgcg
      781 cccagtgcgg ctcgcactgc tacttcccgt tgtcgcgtcg tgctgcgcgc caaaagtaaa
      841 ttcgcaacat ttacgcatgc gataaggagt atgaaaccaa ccgccgccat caagatccac
      901 atgcttttct tcgcttcagg aggcagaaaa tcgttgtgga gcggttccac gctaatatcc
      961 caacccttgt ttgacttggg attgtcctgc gcgatgcggt gggccacgac gttcatatcg
     1021 gcctgcgcct gagccaggga aactccgggc ttcaaacggc cgaaagcaag cagccagtgg
     1081 aagtcatgat tcagctgctc gggcttgaag gtcatcggcg caaaaatatc ccccgccatc
     1141 cggtcaggag ctccgggcgg ccgcaccccg accaccgcgt aaggttcgcc gtcgaggcgc
     1201 agttgtgtcc caattatgtt gggatttgaa cccatgtgtt cccacatccg gtgagtgagg
     1261 atgaccacgt ggtccttccc ggcttgcatc tcgtcgggta gaaaatagcg gcccatccag
     1321 ggcctttccc cgaaaaattt gtcgaagaag ccatacgtcg agcgcgtgcc atccaccatt
     1381 tcgggctggg aaccgggcct cgccaaattg aaactcgcgc cggaagaact caccagagct
     1441 tcaaacgtcg tattctcccg ctgccaatcc tgaaagtcgc ccgcggaaac cacgttgtgc
     1501 ctgccttgta tcttcgacca cagcacaaca agctgcccgg gattgagata gggcatgggt
     1561 tcgaacagag tgg
//
LOCUS       DATCBB010000608         1018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_20029, whole genome shotgun sequence.
ACCESSION   DATCBB010000608 DATCBB010000000
VERSION     DATCBB010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20029"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VII37_06665"
     CDS             complement(<1..544)
                     /locus_tag="VII37_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5069449.1"
                     /translation="MAKRKVKWGVLGAASIAVRKVIPGMQKGAWSEVAAIASRDLNKA
                     KAAAAGLKIEKAYGSYEELLSDPEIEAVYNPLPNHLHVPWSIKAAEAGKHVLCEKPIA
                     LNAAEARTLLAVRERTGVKIGEAFMVKTHPQWLRTRELIREGRIGELRAIVGVFSYFN
                     RNAENVRHKPEWGGGGLLDIG"
     gene            complement(581..>1018)
                     /locus_tag="VII37_06670"
     CDS             complement(581..>1018)
                     /locus_tag="VII37_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069450.1"
                     /translation="SPRALQEGGLDYGWPRCYYSGGKAKVDPRFPSGASRTDCPGAPD
                     AFAFFGAHSSPLGLEYFGVDSTAPALKAQFLVALHGASNKKLGRGYRVVRVDNHGQVQ
                     DFITGFIDHGVVRGRPCDIFKLSEESFLLSDDNAGVIYYVRAK"
BASE COUNT          210 a          373 c          229 g          206 t
ORIGIN      
        1 acccgatgtc caacaacccg cctccgcccc actccggttt atgccgcacg ttctccgcgt
       61 tccggttgaa gtagctgaac acccccacaa tcgcgcgcaa ttccccaatc cgcccttcgc
      121 gaatcaactc tcgggtgcgc agccactgcg ggtgcgtctt caccatgaac gcttcgccaa
      181 ttttcacgcc ggtgcgttcc cgcaccgcca gcagcgtccg cgcttccgcg gcgttcagcg
      241 cgatgggctt ttcgcatagc acgtgcttcc ctgcctccgc cgctttgatg gaccacggca
      301 cgtgcaggtg attcggcaac ggattgtaga cggcctcaat ttccggatcg ctcagcaatt
      361 cctcatacga tccataagcc ttttcaattt tcaacccggc agccgccgcc ttggccttgt
      421 tcaaatcacg cgaagcgata gcggccacct cactccaggc gcccttctgc atccccggaa
      481 tcactttgcg caccgcgatc gacgccgcgc ccaaaacgcc ccacttcact tttctcttcg
      541 ccactcgttc accaatcttt ctagcagcaa cgccccatca tcatttcgcg cgaacataat
      601 aaatcacgcc cgcgttatca tccgatagca agaaagactc ctcgctcaac ttaaaaatat
      661 cgcagggtcg tccccgcacc actccatgat caataaatcc ggtgataaag tcctgcacct
      721 ggccatgatt gtcgactcgc acgacacgat agccgcgacc cagttttttg ttgctcgccc
      781 cgtgcagcgc gacaaggaac tgcgccttca aggccggcgc tgtggagtcc actccaaaat
      841 actcgagccc cagcggcgaa ctatgcgccc cgaaaaacgc gaacgcatcc ggcgcacccg
      901 ggcaatccgt tcgggaagct cccgaaggaa accgcggatc caccttggct ttgccgcccg
      961 agtagtaaca gcgcggccac ccgtaatcta gccctccctc ctgcaatgct cgcggact
//
LOCUS       DATCBB010000609         1603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_311675, whole genome shotgun sequence.
ACCESSION   DATCBB010000609 DATCBB010000000
VERSION     DATCBB010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311675"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1521)
                     /locus_tag="VII37_06675"
     CDS             complement(58..1521)
                     /locus_tag="VII37_06675"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5069451.1"
                     /translation="MFPRTDDVLRALPELIWCGFGMLAMLLQPFVRSRHFFTVLALVG
                     AIGGTGAAFVAGATPGEGFYGLIESDVFSFFFRLLIGLVAVLVVLAANSYLERENLPF
                     AEFYALLLFATAGMGVLASARELLTAFIGLELSSISSYILAGFRRDSMKSSEASLKYF
                     LLGSFATAFFLYGIALVYGATGTTNLSRMDDADPGSNLLKLGLSLILIGIGFKVAAAP
                     FQIWTPDVYEGAPTPVTALFSAGPKAATFALLLRIFASVPAATHFWFWAFWVLAALTM
                     FAGNLGALLQTNVKRLLAYSSIAHAGYILVAFAAVTFMANDLNGGATPAYAAVLFYLL
                     SYALVKIGAFTIVSEIGGMGEKNLTLDDYAGLAQRQPVAAAVLSLYLLSLLGLPVTAG
                     FFGKFYIFKAAVNSHLIWLAVLMAINSIIGAYYYLRVIVFMYMREGSAEAMAAAPVRF
                     PVAVNVVLAVTAIGTVYFGLFPNQVLNFILQPNLIAR"
BASE COUNT          436 a          496 c          389 g          282 t
ORIGIN      
        1 agaaaaaggc ccggaaaaat tccgggcctt tctaattttg gtttaactcc ggcctactta
       61 acgggcaatc aaattaggct gcaaaataaa attcagcacc tggttaggga acaacccaaa
      121 ataaaccgtc ccaatcgccg taaccgccag caccacattt accgccacag ggaaccgcac
      181 aggcgccgcg gccatggctt ccgcgctgcc ttcgcgcatg tacatgaaca caattacccg
      241 caagtagtag tacgccccga taatgctgtt gatggccatc aacaccgcca gccagatcaa
      301 atgtgaattc actgccgctt taaaaatata aaatttccca aaaaatcctg ccgtcaccgg
      361 caatcccaac aacgacaaaa gataaagact caacaccgct gcagctacag gctgccgttg
      421 cgccaggccc gcgtaatcat ctagcgttaa attcttctcg cccatcccgc caatttcaga
      481 aacgatggtg aacgccccaa tcttcacgag cgcataactc aacagataaa acagcaccgc
      541 ggcataagca ggcgtcgcgc cgccattcaa atcattggcc atgaacgtga ccgcggcgaa
      601 cgccactaaa atgtaaccgg cgtgggcaat cgagctatat gccagcaatc gcttcacgtt
      661 ggtctgcagc aaggcgccaa gattcccagc aaacatcgta agcgcggcta gcacccaaaa
      721 cgcccagaac cagaagtgcg tcgccgccgg aacgctcgcg aagattctta acaacagcgc
      781 gaacgtcgcc gccttgggcc ccgcggaaaa tagcgcggtc accggagtgg gtgcgccttc
      841 gtacacgtcc ggcgtccaga tctggaaggg tgccgccgcc actttaaatc caatgccaat
      901 cagaatgagg gacaagccca gtttcagcag gttgctgcct gggtcggcat cgtccatcct
      961 gctcaggttc gtggtgcccg tcgcgccgta aaccagcgcg atgccgtaaa gaaaaaacgc
     1021 ggtggcaaat gatcccaata aaaaatattt gagcgacgct tcgctggatt tcattgagtc
     1081 gcggcggaat cccgccagga tgtaactgga aatcgagctc aactccagtc cgatgaatgc
     1141 ggtcaacaat tcacgcgcgg aagccaaaac tcccatgccc gctgtggcga ataggagcaa
     1201 tgcataaaat tccgcgaatg gtagattctc gcgttccagg tatgaattcg ccgccagcac
     1261 caccaggacc gccaccaacc cgatcagcag ccggaagaaa aaactaaaaa cgtcggactc
     1321 aatcagcccg taaaatcctt caccaggcgt agcgccggcc acaaacgccg cgccggtgcc
     1381 gccaatggcg cctaccagcg cgagaactgt aaaaaagtgc cggctgcgca caaaaggctg
     1441 cagcaacatc gccagcatgc caaagccgca ccagatcagc tccggtagcg cgcgcagcac
     1501 gtcatcggtg cgtgggaaca ttagcggttc gcgccctctt ccgccggaat ttctgcggcg
     1561 ggaatctccg cgacattctg cgaattattt gtcctgccca ccg
//
LOCUS       DATCBB010000610         1579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_150186, whole genome shotgun sequence.
ACCESSION   DATCBB010000610 DATCBB010000000
VERSION     DATCBB010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150186"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VII37_06680"
     CDS             <1..620
                     /locus_tag="VII37_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069452.1"
                     /translation="SLDVPGDLSSAVFFIAAASLFPESSLLIHNVGLNPTRTAILDLF
                     LSMGASIQTPTLRSAQGELVGDLAVKGAQLKGGVIEGDQIALLIDELPMLAALGPYTE
                     NGIEIRDAKELRVKESDRIAALVENLKRMGATVEERPDGLKVEGRKAGKLRGAEIDPH
                     GDHRIAMAFAVAGLAAEGTTVILGAECAGVSYPTFFEDLTRVAER"
     gene            complement(681..>1579)
                     /locus_tag="VII37_06685"
     CDS             complement(681..>1579)
                     /locus_tag="VII37_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY5069453.1"
                     /translation="YEIHPTGSNTGDSRLGFDMEIVVPKKSQLTVRNEKGDVVVSDIA
                     APVIIDNHNGDVDIRNTTGDVTIDMRHGDVKVADTKGDIKLAGKGGEVDVTSASGGLT
                     VDGEFYGPIRADKIAKGVRYISQRSDLTLSQLGGHLELGSGNLEITDAPGNLQLRTNR
                     YDVNVENVGGKAKIENRDGTVELRFSSQPKDDIDITNANAVISLSLPASSSFEISADC
                     HSCDIDSDFSGGTLSKSSGGSGDNHLQGKYGTGRATKITLKTSYGNISLRKTSGDSMQ
                     PPAPPHVPNPPHAAEIPEPEEN"
BASE COUNT          323 a          375 c          493 g          388 t
ORIGIN      
        1 aatcgctgga cgttccggga gatttatctt cggcggtatt ttttatcgcc gccgcttcgc
       61 tatttcccga gtcgagcttg ctgattcaca atgtggggtt aaatccgacg cgcacggcga
      121 ttctggattt atttttgtcc atgggggcgt ctattcaaac gccgacattg cgcagcgcac
      181 aaggcgaact ggtgggcgac ctggcggtga aaggcgcaca gctgaagggc ggggtgattg
      241 agggagatca aatcgcgctg ctgattgatg aattgccgat gctggcggcg cttgggcctt
      301 acacggaaaa cggaattgag attcgcgatg ctaaggaatt gcgcgtcaag gaaagcgatc
      361 gcatcgcggc gctggtggaa aatttgaagc gcatgggcgc taccgtagaa gaacggcctg
      421 acggacttaa agtggaggga cgcaaagcgg ggaagttgcg tggcgcggaa atcgatcccc
      481 acggcgacca tcgtattgcg atggcttttg cggtggcggg gctagcggcg gaaggaacga
      541 ccgttattct cggggcggaa tgcgcgggag tttcttatcc gacgtttttt gaggatttga
      601 cacgagtggc ggaacgatag ggccgaagcg cgctaagacg cgagagattc gcgtgtgagg
      661 ttttctaact ttctaatttt ctaattctct tctggttcgg gaatctcggc tgcgtgtggg
      721 gggttaggaa cgtggggagg ggccggtggc tgcatggaat cgccgctggt cttgcgcagc
      781 gaaatgtttc cgtaacttgt tttcaaagtg attttagtag ctcggccagt gccgtatttt
      841 ccctgcaagt gattatcccc tgagccgccc gaggatttag atagcgtacc acctgagaag
      901 tccgaatcga tgtcgcagga atgacagtcg gcggagatct cgaaacttga agaggcgggc
      961 aagctcaagg aaataaccgc gttggcattg gtgatgtcga tgtcatcttt gggctgggaa
     1021 gaaaatcgca gctccacggt gccatcgcga ttctcgattt tcgctttgcc gccgacgttt
     1081 tcgacattga catcgtaacg attggtgcgc agctgcagat ttccgggcgc atcggtaatt
     1141 tcgagattgc cggaacccaa ttccaggtgg ccgccgagtt ggctgagggt caaatcgctg
     1201 cgttgcgaaa tataacgcac acccttagca attttgtcgg cgcgaatggg cccgtagaat
     1261 tcgccgtcga ccgtgaggcc gccggatgcg ctggttacgt ccacttcacc gcctttgccg
     1321 gccagcttga tatcgccttt ggtatcggca acttttacgt cgccgtggcg catgtcaatg
     1381 gtgacgtctc cagtggtatt gcggatgtcg acgtcaccat tgtgattgtc gatgatcacc
     1441 ggggcggcta tgtcggaaac caccacgtcg cctttttcgt tgcgaaccgt tagctgcgat
     1501 tttttcggga cgacaatttc catatcgaag cccagccgcg agtcgccagt attcgatcca
     1561 gtgggatgaa tttcgtagc
//
LOCUS       DATCBB010000611         1142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_376514, whole genome shotgun sequence.
ACCESSION   DATCBB010000611 DATCBB010000000
VERSION     DATCBB010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376514"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VII37_06690"
     CDS             <1..643
                     /locus_tag="VII37_06690"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY5069454.1"
                     /translation="NAVLHSFVTVYRGAKIGSDFFAHAHAVVREFCVVGDRVILQNGV
                     IIGGDGLGFAKRKDGSWYKIAQSGVAVLEDDVEVQANACVDRATVGETRIGRGSKLDD
                     LVLVGHASKVASNAMLCGQVGLAGSTQIGHGVILAGQVGTAGHLSVGDHAVVTAKSGI
                     PNDVPARAMYSGYPAVDNRQWLKSVAALNRLPELQKRVWELEEEIEKLKGSKS"
     gene            complement(654..>1142)
                     /locus_tag="VII37_06695"
     CDS             complement(654..>1142)
                     /locus_tag="VII37_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5069455.1"
                     /translation="VVGAIPRRIAILLKESLFRWPIVGQAFRLAHFIPIERGGRDSAI
                     ASLDKATEELRAGQSFLIYPEGTRSPDGRLQKFKRGAVVMAIKAGAPIVAVACSGAHR
                     LMKKRSLVLRPGEILVEFLPPIDASAYTLETRDKLNDLVHDTLAAALPSDQRPIGFPG
                     AG"
BASE COUNT          247 a          318 c          342 g          235 t
ORIGIN      
        1 taatgcggtg ttacatagct ttgttacggt ctaccgaggc gcaaagattg gctcggactt
       61 tttcgctcac gcgcatgcgg tggttcgcga gttttgcgtc gtcggcgatc gcgtcatctt
      121 acaaaatgga gtgatcatcg gtggcgacgg tttgggcttc gcaaaacgaa aagatggcag
      181 ttggtacaaa attgcgcaat ctggtgtcgc ggttcttgag gacgatgtgg aagtgcaggc
      241 caatgcctgc gtggaccgcg ctactgtggg cgagacgcgc atcggccgtg gctccaagct
      301 ggatgacctc gttctcgtgg gtcacgcatc caaagtcgcc agcaacgcga tgttgtgtgg
      361 ccaggttggt ttggcgggct ccacgcagat cggtcacgga gtaattcttg cggggcaggt
      421 cggcaccgcg ggacatctct cggtcggcga ccatgccgtg gtcacggcca agagcggaat
      481 ccccaacgac gttcctgctc gcgccatgta ttccggttat cccgcagtag acaaccggca
      541 atggttaaag tccgtcgcgg cgctgaaccg cttgcctgaa ctgcaaaagc gcgtgtggga
      601 attggaagaa gaaattgaaa aactaaaagg gagtaaaagt tagctttgcg aacctaccct
      661 gccccaggaa atcctatcgg gcgctggtcg gacggaagtg ccgccgccaa agtgtcgtgc
      721 accaggtcat tgagcttatc gcgagtctcc aacgtatagg ccgacgcgtc gatgggcggc
      781 aaaaactcca ccagaatttc gcccggccgc agaaccagcg agcgcttctt catcagtcga
      841 tgagcaccgg aacacgccac tgccacgata ggcgcaccag ctttgatggc catcaccacc
      901 gcgcctctct tgaatttctg caaccgccca tccgggctgc gcgtgccttc cggataaatt
      961 aaaaacgatt gtcctgcgcg cagctcttcc gtggctttgt ccaggctggc tatggcggag
     1021 tcacggccac cgcgttcaat cggaatgaag tgcgccagcc ggaaagcctg ccctacgatg
     1081 ggccagcgaa aaagcgattc cttcaagagg atggcaatgc ggcgcggaat tgcaccgact
     1141 ac
//
LOCUS       DATCBB010000612         1153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_376539, whole genome shotgun sequence.
ACCESSION   DATCBB010000612 DATCBB010000000
VERSION     DATCBB010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376539"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VII37_06700"
     CDS             complement(<1..268)
                     /locus_tag="VII37_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5069456.1"
                     /translation="MRLEGADRKPLHRDVIRGLQNILGAYMVLHRPEDLLLYEYDGSV
                     EKGRPGAVVFPTTTHEVSRVVRLAAEHDIPIIGRGAGTGLSGGAL"
     gene            412..>1153
                     /locus_tag="VII37_06705"
     CDS             412..>1153
                     /locus_tag="VII37_06705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY5069457.1"
                     /translation="MPRPKLFATRPLFEEARRILQEKCQVEYWPEPGRISRQDLAARV
                     QDKQGLVCLLTERIDETFLNAARELRIVANVAVGFDNIDVPACTKRGIAATNTPGVLD
                     ETTADFAWTLMMAVARRLGEGEALARSGKWLGWDLDQLVGADVWGKTLGLVGFGRIGR
                     AMARRASGFKMKVLYTDVARVSEDLEKELNAEYRDLDTLLAQSDFVSVHVPLLPETQD
                     LFTSAKFARMKPTAFFINTARGSVVDEAA"
BASE COUNT          255 a          326 c          330 g          242 t
ORIGIN      
        1 ccagcgcgcc tcccgaaaga cccgtgcccg cgccacgccc gatgatggga atgtcgtgtt
       61 ccgcagctaa tcgcacaact cgcgacacct cgtgcgtcgt tgtagggaaa accactgcac
      121 ccggccggcc tttttccacc gagccgtcat attcatacag caataaatct tcgggccggt
      181 ggagcaccat gtaagcgccc agaatatttt gcaacccgcg aatcacgtcg cgatgcagag
      241 gttttctatc agcgccttct agtcgcacgt taaatagccc cttcccacta tagtgagcct
      301 cgcgccaccc tggcaatcgc aggcacgata atttgcttac acgcggtgca agtgcccggc
      361 tctgtggaac ctgtgctagt gtatttttcc agtctcttca gaggaagatg gatgccccga
      421 ccaaaacttt tcgccacaag accgttgttc gaagaagcgc gccgcatctt gcaagaaaaa
      481 tgtcaggtgg agtattggcc ggagccgggg cgcatctcaa gacaagacct tgccgcccga
      541 gtgcaagata aacagggttt agtgtgcctg ctgacagaac gcatcgacga aacatttttg
      601 aatgccgcgc gcgaactccg catcgtcgcc aacgtcgctg tgggcttcga caatatagat
      661 gttccggcgt gcaccaagcg cggcattgcc gcgaccaata ctcccggcgt ccttgatgaa
      721 accaccgcgg actttgcgtg gacgttgatg atggccgttg cgcgacggct cggcgaaggt
      781 gaagctctcg cgcggtccgg caagtggtta ggttgggacc tggaccaact ggtgggtgcc
      841 gacgtatggg ggaaaacgct tggcctggtt ggcttcggac gtatcggacg ggccatggcg
      901 cgcagagcga gcggattcaa aatgaaagtt ctgtacaccg atgtggcgcg agtatcggaa
      961 gatctcgaaa aggaattgaa cgcagaatat cgcgatttgg ataccttgct ggcgcaatcc
     1021 gatttcgtca gtgtgcacgt gcctttactg ccggaaactc aagacttgtt caccagcgcg
     1081 aaatttgcgc gcatgaagcc gaccgccttt ttcatcaaca cagcgcgcgg ctcggtggtg
     1141 gatgaagcgg cgc
//
LOCUS       DATCBB010000613         1140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_52962, whole genome shotgun sequence.
ACCESSION   DATCBB010000613 DATCBB010000000
VERSION     DATCBB010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52962"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VII37_06710"
     CDS             complement(<1..487)
                     /locus_tag="VII37_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069458.1"
                     /translation="MPSVIALDAMGSDKAPKPEIEGALQAARHHGVRVLLVGPEARLR
                     ADLERYPGARRLQIEVVHASEVITMDDKVDAIRAKRDSSIRVGLRLVREGRADGFVTA
                     GNTGAAMATAKVVLGALPGVDRPALAAAFPTVNNTAAVLLDVGANVDCTAVNLEQFAV
                     MG"
     gene            complement(500..685)
                     /gene="rpmF"
                     /locus_tag="VII37_06715"
     CDS             complement(500..685)
                     /gene="rpmF"
                     /locus_tag="VII37_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY5069459.1"
                     /translation="MPNPKRRHSQKRTATRRAHDSLSKHSLSECPNCHERKMPHRACP
                     KCGTYKGREVVEVDEKS"
     gene            complement(778..>1140)
                     /locus_tag="VII37_06720"
     CDS             complement(778..>1140)
                     /locus_tag="VII37_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY5069460.1"
                     /translation="LDTSLEVPCARCLDPVVHPVERSFDLLYRPLGIDSGHAELSVTD
                     AEAEIGYYENSGLLLEDTLREQVLLALPLKTLCREDCKGLCPHCGKNMNEIECGCVDE
                     LEDPRWSALKEIRDKLNQ"
BASE COUNT          214 a          350 c          322 g          254 t
ORIGIN      
        1 cgcccattac cgcgaactgc tccagattaa ccgcggtgca atcgacattc gcgccgacgt
       61 cgagcagaac ggctgcggtg ttgttgactg tcggaaacgc cgctgccagc gcgggacgat
      121 ccactccggg cagcgcgccc agcactactt ttgcagtggc catcgctgcg cccgtgttgc
      181 cggccgtaac aaagccgtcg gcgcggcctt cgcgcaccag gcgcaatccg acgcggatcg
      241 acgaatcgcg cttggcgcgg atcgcatcca ctttgtcatc catggtgatg acttcgctcg
      301 catgcacgac ttcgatctgc agccggcgcg ctcccggata acgctccagg tcggcgcgca
      361 atcgcgcctc gggaccgacc aaaagcacgc ggacgccatg atggcgtgcc gcttgcagcg
      421 cgccctcaat ttcgggcttc ggcgctttgt cggaccccat cgcatccagg gcgattacgc
      481 taggcatggg aagaagaaac taggactttt cgtcgacctc tactacttcg cggcccttgt
      541 aggttccgca cttggggcag gcgcggtgcg gcattttgcg ctcatggcaa ttcgggcact
      601 ccgacaagga gtgcttcgaa agggaatcgt gggcgcgccg cgtggcggtg cgcttctggg
      661 aatgtcttcg ttttggatta ggcatgatgt ccttttttag ttctcagttc ccagttctcg
      721 gttctcagta gagctgcttt tactgagaac tgagaactgg gaaccgaaaa ctcgtgtcta
      781 ttgattcagt ttatcgcgta tctctttcag cgccgaccac cgcggatctt ctagttcgtc
      841 cacgcatccg cattctattt cattcatgtt cttgccgcag tgcggacata accctttgca
      901 gtcctcgcgg cacagcgtct tcagcgggag ggctaaaagc acctgctcgc gcagcgtatc
      961 ctctagcagc aggccgctgt tttcgtagta tccgatttcc gcttcagcat ccgtgactga
     1021 aagctcggcg tgaccggagt caattcccag cggccgatac agcagatcga aactgcgctc
     1081 gactggatgc acaaccggat caagacaacg tgcgcaaggc acttccaagc tcgtatcgag
//
LOCUS       DATCBB010000614         1310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_118234, whole genome shotgun sequence.
ACCESSION   DATCBB010000614 DATCBB010000000
VERSION     DATCBB010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118234"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VII37_06725"
     CDS             <1..501
                     /locus_tag="VII37_06725"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY5069461.1"
                     /translation="PANRQTVDPNRPPSSQYPGGGQYSRDGQYSRGGQNNYNTYKNNQ
                     QHFSPEERQRVLQNEQRFRQLSPQQQQDMRDRQRVWQQMTPQQRDHIKYDVLPTWRNL
                     PPQRKKAIEQRLGVLQNMPEFARNQHLNDPNFTRGMSPEDRATLRDLSHLHVGGAPDP
                     PTNNPD"
     gene            766..>1310
                     /locus_tag="VII37_06730"
     CDS             766..>1310
                     /locus_tag="VII37_06730"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5069462.1"
                     /translation="MSTRFRALQHRNFQLFIGGQLVSLIGTWMQSTAQLWLVYKLTGS
                     AALLGVFGFANQVPILFLASVGGYVGDRYNRHYGVIATQAVLMLLAFALAALTFAEAI
                     KVWELIFIAFLVGVVNAFDVPIRQSFLVQMVVKEDLPNAIALNSSIFNGARVVGPAVA
                     GFALAWKGPAWCFLLNGISFLA"
BASE COUNT          289 a          363 c          349 g          309 t
ORIGIN      
        1 cctgccaatc gtcagacagt cgatcccaat cggccgccga gcagtcaata tcccggaggt
       61 gggcaatatt cacgagatgg gcaatattcg cggggcgggc aaaataatta caatacctat
      121 aaaaataacc agcaacactt cagccccgaa gagcgacagc gagtgctcca gaacgagcaa
      181 cgctttcggc aattgtcgcc gcaacagcaa caggatatgc gcgaccgcca gcgggtgtgg
      241 cagcagatga cgccgcagca acgcgatcat attaaatatg acgtgctgcc cacgtggcgc
      301 aatttgccgc cacaacgaaa gaaagccatc gagcaacgtc tgggcgtgct ccagaacatg
      361 ccggagttcg cgcgaaatca acatctgaac gaccccaatt ttactcgtgg gatgagtcca
      421 gaagatcgcg ctacgctgcg cgatctgagc catctgcatg taggtggcgc acctgatccg
      481 ccaacgaata atcccgatta gcgcaagcat tcagtgcatc gggctgaata gctaaacttc
      541 tatccgcgaa actgccagca atcttgttgc ggctgacacg gggaaacaac tggaagccca
      601 tcggaacttt gtacagcttc acggtcacaa agtcgtcact ttgcttcatc tcacacggtt
      661 ggctttcact cctccggtag ccatccgtcc gttacactgg ttagtgatgc gttgcgtgac
      721 gcgccatcaa cccacgcatc gaactggaga ggaatttttc cgctagtgtc tacgcgcttc
      781 cgcgccttgc agcaccgcaa ctttcagctt ttcatcggcg gccaacttgt ttcactcatc
      841 ggcacgtgga tgcagtccac tgcgcagttg tggctggttt ataaacttac gggatcggcc
      901 gctctattgg gcgtttttgg atttgccaac caggtgccaa ttctatttct cgcttccgtc
      961 ggcggttatg tgggcgaccg ctataaccgg cattacggag tcatcgcgac ccaggcagta
     1021 ttgatgctgc tggctttcgc tctcgcggct ctgaccttcg ccgaagctat caaagtgtgg
     1081 gagttgattt tcatcgcgtt tctggttggc gtagtcaacg cctttgacgt gcccatccgc
     1141 cagtcatttc tggtgcaaat ggtggtgaaa gaggatttgc cgaatgccat cgctctgaac
     1201 tcttcgatct tcaatggcgc tcgggtggta ggacccgcgg ttgcgggctt tgcgttggcc
     1261 tggaaaggac cggcatggtg tttcttgctg aatgggatca gtttccttgc
//
LOCUS       DATCBB010000615         1237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_441328, whole genome shotgun sequence.
ACCESSION   DATCBB010000615 DATCBB010000000
VERSION     DATCBB010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441328"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..>1237)
                     /gene="hemA"
                     /locus_tag="VII37_06735"
     CDS             complement(21..>1237)
                     /gene="hemA"
                     /locus_tag="VII37_06735"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5069463.1"
                     /translation="SYTAEQARRAATELRSRGILEETLVLSTCNRSEVYGVPPETSHE
                     SAAGLSSFLSEFHKVQPDILSVSLYHHYDRQAVRHLFRVSAGLDSMMLGEAEILGQVR
                     EAYKFAYEQGATGPVLNRMFQAALEVGKRVRSETELGTRPMSVASAGVKLAERIFGKL
                     GQRTALVLGAGTISEQVLQQLRDRGIGQLLMMNRSRERAEKLATEFGGTVMSWGEWAE
                     ALKAPDVIVSSVTAEEPILERSILERAMSTRGNRALFIMDLGLPRNIEPAAAEIYNVY
                     LYNLDDLGGIVQQSKAMRENEIPRAETIVDEHVGKFLSWQASVELVGLVDELRTRLQH
                     ERSAFIHERMESMKHLSAQDRQQMEALMGELLEKLLLEPAQRLRGEKELRRKIQNVEA
                     LRDLFLSNREKQ"
BASE COUNT          265 a          379 c          298 g          295 t
ORIGIN      
        1 tgttccgatc cgtaaatgct tcattgtttc tcccgattgg aaaggaacag atcgcgcaat
       61 gcttctacgt tctgaatctt gcgccgcaat tctttttcgc cacgcaagcg ctgcgcaggt
      121 tccagcaata atttctccag cagctcgccc attaacgctt ccatctgttg gcggtcttgc
      181 gcgctcaaat gcttcatgct ctccatgcgc tcgtggatga aagcactgcg ctcgtgctgt
      241 aaacgagtac gtaactcgtc tactaagccc actaactcga cgctagcctg ccaggaaaga
      301 aatttgccca catgctcgtc gacaattgtc tccgcccgcg gaatttcgtt ttctcgcatg
      361 gctttgcttt gctgcacaat gccgccgagg tcgtccaagt tataaaggta cacattgtag
      421 atttcagccg ccgccggctc gatattgcgt ggcaaaccga gatccataat gaacaacgcg
      481 cggttgccgc gggtggacat cgcgcgctcg agaattgagc gctccagaat gggctcctcc
      541 gcggtgacgg atgaaacgat cacgtctgga gccttcagcg cttctgccca ttcaccccac
      601 gacatcactg tgccgccgaa ttccgttgcc agtttttcag cgcgctcccg cgaacggttc
      661 atcatcaaca actgccctat gccccggtcg cgcaattgct gaagcacttg ctcgctgatc
      721 gttccggctc cgagcaccag cgcggttcgt tgaccgagct tgccgaaaat tctttctgcc
      781 aatttcacgc cggcagaggc taccgacatt ggccttgtgc ccaactcggt ttctgaccgg
      841 acgcgcttcc ccacttccaa tgcagcttga aacatacgat ttaagaccgg tccggttgca
      901 ccttgttcat aggcgaattt atacgcttcc cgaacttgac ccagaatttc cgcttcaccc
      961 aacatcatgg aatccaagcc ggcggaaact cggaataagt gtcgcaccgc ttgccggtca
     1021 tagtggtgat agagcgagac actcaaaata tcgggctgta ccttgtgaaa ctcacttaaa
     1081 aaagacgaaa gccctgccgc gctctcgtgt gaagtctctg gcggcacccc gtacacctcg
     1141 ctgcgattac aggtcgacaa aaccaatgtt tcttcgagga ttcctcgcga acgcagctcg
     1201 gtagccgcgc gtcgcgcctg ctccgcggtg taagaga
//
LOCUS       DATCBB010000616         2574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_441331, whole genome shotgun sequence.
ACCESSION   DATCBB010000616 DATCBB010000000
VERSION     DATCBB010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441331"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VII37_06740"
     CDS             <1..1212
                     /locus_tag="VII37_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY5069464.1"
                     /translation="NLKAMLEAATPESLVLVDEMGTGTAPEEGAALAVALLDEFRAKN
                     CIVLATTHQDRLKTYASTTPGVVNAAVEFDDVNLRPTYRLMVGVPGGSSGIAIARRLG
                     IAEPIIGRARSLMAPEALEAADLIAYLHRSRDELDRMQQQMTAERHALDEERKKLRDE
                     WVGRQQSRIRELEEKFAEMQKRFDENVARVVEAVKERELRGAVEKFARRKAQDVRGEA
                     RDELNAAVVQTISESQQDLGAPAAGKESLTQAQLQSGTRIHVRGFSKPVVLRRIDGIS
                     AEIEAGPLRMKVGIDEITGIQGASTASTPRSSKGSITVRAQASDEGTGGEINLIGCTV
                     EEATERVDKFLDEAALANRSRVRIIHGHGTGALRKGLAQFLSKHPLVDTQSFETEERG
                     GKAVTIVELRA"
     gene            1213..>2574
                     /gene="dnaG"
                     /locus_tag="VII37_06745"
     CDS             1213..>2574
                     /gene="dnaG"
                     /locus_tag="VII37_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5069465.1"
                     /translation="MAEAGSFAERVKQQADIVRVIGEYVRLKKSGQNFTGLCPFHAEI
                     SPSFAVHPTKQIYHCFGCGVGGDVFKFVMEMDKVAFPEAIRVVAEKCGIAIPRPKSSS
                     PEERKENQQRSLLVEMHREARTFFVQQLGGTLEGKAARAYLEDRGLDKDAIARFGIGY
                     APSGGDALLRFLKSKYKEPLLIESGLVSRDQSGARMYDRFRRRITFPIANESGKIVAF
                     GCRALGDDQPKYLNSPETPIYSKSNVLYHLDRAKEALRRADFAVLVEGYMDAIAVARA
                     GISNVVASCGTSLAEPQIKLLSRFTKRVIVNYDPDTAGQLATERSISLLLENDFEVRV
                     LALPAMNLTAGDGTKYSKNADPDLYIREMGVEAYTKLLKDAPPYVDYLIGRARHMDLS
                     TGEGKLRAVNFLLPYVQKIPNRLLRSEWATRIAQQLRLEEPVLRAALSKAAAERRSGL
                     KVAP"
BASE COUNT          616 a          662 c          805 g          491 t
ORIGIN      
        1 aatctgaaag ccatgctgga agcggccacg cccgaatccc tggtattagt cgatgaaatg
       61 ggcacgggta ctgcgccgga agaaggagcg gcgctggcgg tggccttgct ggatgaattt
      121 cgcgcgaaaa attgcatcgt actggcgacc acgcatcaag accggctgaa aacgtacgcc
      181 tcgacaacgc caggagtggt gaacgccgca gtggaatttg acgacgtgaa tcttcgaccg
      241 acgtaccggc tgatggttgg tgtgccgggc gggtccagcg gaatcgccat cgcgcgtcga
      301 ttaggtattg ccgagccaat catcggccgt gcgcgttcgc tcatggcccc ggaagcgctt
      361 gaagccgcgg acttaattgc ctacctgcat cgcagccggg atgagctgga ccgcatgcag
      421 cagcagatga ccgcggagcg gcatgcgctc gatgaagaac gcaagaaatt acgcgatgag
      481 tgggtggggc ggcagcaaag ccgcattcgc gaactagaag aaaaatttgc agagatgcaa
      541 aagcgcttcg acgagaacgt cgcgcgcgtt gtggaagcgg taaaagaacg ggaattgcgc
      601 ggagcggtcg agaagttcgc gcggcgcaaa gcgcaggatg tgcggggcga agcgcgcgac
      661 gagttgaatg ccgcggtagt gcagaccatt tcagaatcgc aacaggacct tggtgcaccc
      721 gcggctggga aagagagcct tactcaagcg caattgcagt cgggcacgag aatccacgtg
      781 cgcggtttta gcaagcccgt ggtgttgcgg cgcattgatg ggatttcagc ggaaattgaa
      841 gccgggccgt tacgcatgaa agtaggaatc gatgagatca ccggcatcca aggagcgagc
      901 accgccagca ctcctcgatc gtcaaaaggc agcattaccg tgcgcgcgca agccagcgat
      961 gaaggaacgg gcggcgaaat taatttgatc ggttgcaccg tggaagaagc cacggaacgc
     1021 gtggataaat ttctggacga agcggcactg gccaaccgct cgcgagtgcg aattattcac
     1081 ggacatggca ccggagcgtt gcgtaaaggg ctggcgcaat ttctttccaa gcatccgttg
     1141 gtagataccc aatcgtttga aaccgaagag cgcggcggca aggccgtcac cattgtggag
     1201 ttgcgggctt aaatggcgga agcgggatca ttcgcggagc gcgtgaagca gcaggcggac
     1261 attgtccggg tcatcggcga atacgtgcgg ctgaaaaaga gcggtcaaaa ttttaccggg
     1321 ctttgccctt tccatgcgga gatatcgccc tcgtttgcgg tgcaccccac aaaacagatt
     1381 taccactgct ttggctgcgg ggtgggcgga gacgttttca aatttgtaat ggagatggac
     1441 aaggttgcgt ttcccgaagc gatacgcgtc gtggcggaaa aatgcggaat cgccattccg
     1501 cgcccaaagt ccagttcgcc ggaagagcgc aaagaaaatc agcagcgttc tttgttggtg
     1561 gaaatgcatc gcgaagcgcg aacatttttt gtgcagcaac tgggcggaac gctggaagga
     1621 aaagcggcgc gagcgtacct tgaggatcgc ggcttggata aagatgccat cgcgcgcttc
     1681 gggattggtt atgctccaag cggcggcgac gcgctgctgc gatttctcaa atcgaaatat
     1741 aaagagccgt tgctgattga atcgggcctg gtatcccggg atcaaagcgg cgccaggatg
     1801 tatgaccgct tccgcaggag aatcacgttc ccaatcgcta acgaatcggg aaaaatcgtg
     1861 gcctttggat gccgcgcgct gggcgacgac cagccaaaat acctgaactc gccggaaact
     1921 ccaatttatt cgaagagcaa cgttttgtat cacctggacc gcgccaagga agcattgcgg
     1981 cgtgcggatt tcgcggtgct cgtggaaggc tacatggatg cgatcgccgt ggcgcgtgcc
     2041 ggcatcagta atgttgtggc cagttgcggc accagtttgg cggagccaca gatcaaactt
     2101 ctttcgcgct tcaccaaacg cgtgatcgtc aactacgatc ccgataccgc gggacaactc
     2161 gccaccgagc gctctatttc gttactgctc gaaaatgact ttgaagtgcg cgtgctggcc
     2221 ttgccggcaa tgaacttaac ggccggcgac ggcaccaaat actcgaagaa cgccgatccg
     2281 gacctttata ttcgcgaaat gggcgtcgag gcctacacca aattattaaa agatgctccg
     2341 ccttatgtgg attatttgat cgggcgggca cggcacatgg acctgtcaac cggcgaagga
     2401 aaattgcgcg cagtgaattt tcttttgccg tacgtgcaga aaattccgaa ccgcttgttg
     2461 aggtccgaat gggccacacg catcgcgcaa caactacggc tcgaagaacc cgtgttgcga
     2521 gcggcgctga gcaaggccgc cgcggaacga cgcagcgggc taaaagtggc tcct
//
LOCUS       DATCBB010000617         1526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_246642, whole genome shotgun sequence.
ACCESSION   DATCBB010000617 DATCBB010000000
VERSION     DATCBB010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246642"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VII37_06750"
     CDS             <1..728
                     /locus_tag="VII37_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5069466.1"
                     /translation="VNEQFAKHYWPGEDAVGKHIRLDSRDGTPVEIVGIAQNIKYHST
                     AERPIDFIYMPLAQHPVPRMTLMLRSSGDPLQLVGPLKDVVRTLDANLPMLDTRSYEE
                     YYRNQAVEGPRIAMKLVGSMGAVGLLLTIAGLYGLVAYNASRRTREIGIRMALGAARS
                     DVMRLVMGKGLVLVAVGTALGLVMGFAIEHLMNSMLFNAGGVDVAAYVAVVPVMFLVT
                     MLAAFVPARKASRIAPTQALRYE"
     gene            853..>1526
                     /locus_tag="VII37_06755"
     CDS             853..>1526
                     /locus_tag="VII37_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5069467.1"
                     /translation="METYDIICFGAGPTGLATAIEARRAGMRSLVIDKGTLCNSIFNY
                     PVNMYFFTTPELLEIGDLPLVCAAEKPTRVEALKYYRKCAEHFELELRLFELIEGLEG
                     SDGNFTVRTRAQDGTQQQYHGRKIAIATGYYDLPNMLGVPGEDLPNVSHYYTEPHPYW
                     RQDVVVVGGKNSAAEAALDLYRNGARVTLVHRDAELGSAIKYWVRPDIENRIKAKQIT
                     GLFNTRV"
BASE COUNT          314 a          345 c          520 g          347 t
ORIGIN      
        1 tcgtgaacga gcaattcgcg aaacactatt ggccaggcga ggatgcggtg ggcaaacaca
       61 tccggctcga tagccgcgac ggcaccccgg tggagatcgt agggattgcg cagaacatta
      121 agtaccattc gaccgccgaa cggccgatag atttcatata tatgccgttg gcccagcacc
      181 ctgtcccgag gatgacgttg atgttgcgct ctagcggcga cccactgcaa ttggtggggc
      241 cgctgaagga tgtggttcga acgctggatg cgaatctgcc gatgttggat acgaggtctt
      301 acgaggagta ttaccggaac caggcggtgg aagggcctcg aattgcgatg aaactggtgg
      361 gttccatggg cgcggtggga ctgctgctga cgatcgctgg gctgtatgga ctggtggcgt
      421 acaacgcgag ccgcaggacc cgcgagatcg ggatacggat ggcgctgggc gcggcaaggt
      481 cagatgtaat gcggcttgtg atgggcaagg gcctcgtgct ggtggcggtc ggaacagcgc
      541 tcggattggt gatggggttc gcgatcgaac accttatgaa ttcgatgctg ttcaatgccg
      601 gcggcgtaga tgtcgcggct tacgtcgcgg tcgtgccggt gatgtttctg gtgaccatgc
      661 tggcggcttt tgtgcccgcg cgtaaagcat cgcggatcgc tccaacgcag gcgctacgat
      721 acgagtagcg cggtcggttt ctgaaaccgt tgctcatcct cgacacggtc ttagcgtaaa
      781 ttcctcccta taaaatgcgc ctggttttgg gtcggtttgc aattgtggag gcgagactgc
      841 aaactgaaat tcatggaaac ctacgacatt atttgctttg gggctgggcc gactgggctg
      901 gctaccgcta ttgaggcacg gcgtgcggga atgcgttcgc tggtgattga taagggtacg
      961 ctgtgcaatt cgatttttaa ttatccagtg aacatgtatt tctttacgac gccggagtta
     1021 ctggagattg gggatttgcc gctggtttgc gccgcggaaa aaccgacacg tgtagaggcg
     1081 ctgaaatatt accgtaagtg tgcggagcat tttgaactgg aattgcggct gtttgagttg
     1141 attgaggggc tggagggcag cgacgggaat tttaccgtga ggactcgcgc gcaggatggg
     1201 acgcagcagc agtatcacgg aaggaagatc gcgattgcga ccgggtatta cgacttgccg
     1261 aacatgctgg gtgtgccggg cgaggatctg ccgaatgttt cgcactacta cactgagcct
     1321 catccttact ggaggcagga cgtggttgtg gtcgggggga agaattcggc ggcggaagcg
     1381 gcgctggatt tatatcggaa cggcgcgcgc gttacgctgg tgcatcgcga cgctgagttg
     1441 ggatcggcga ttaaatattg ggtgcggccg gatatcgaga atcggatcaa ggcgaaacag
     1501 attaccgggt tattcaatac gcgggt
//
LOCUS       DATCBB010000618         6186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_118295, whole genome shotgun sequence.
ACCESSION   DATCBB010000618 DATCBB010000000
VERSION     DATCBB010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118295"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VII37_06760"
     CDS             complement(<1..1265)
                     /locus_tag="VII37_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5069468.1"
                     /translation="MANRTILEEERATKSSKSTKKTPNGSSKAGGVAERERVLELFRR
                     WGFYESNLDPLGFLEPVKRPELNLSGPAADEARAIYCGTIGAEFMHLPEQERRQWIAE
                     RLESPAPAVDQRQILERLVRADLFEQVLQARYLGAKRFSLEGVTALIPLLDALLESSA
                     ETGAQESVIAMSHRGRLNVMVHTACKPAHQVVSGFEDVDPRSVLGAGDVKYHVGATGV
                     FKTTTGKQIGIHLVSNPSHLEAVDPVAMGRARAKQTRLGGDSTKAIVPIVMHGDAAFA
                     GQGIWAETLNLADLKAYTVGGTIHIVVNNLIGFTTRPVQTQSSRFASDIAKQQSVPVF
                     HVNAEDPDAVVRVGKIAAEYRAKFGSDVVVDIIGYRRHGHSEVDDPTITQPLLYEKIK
                     RHPQLWKLYAKQTGIDPAAMAEEIRAEYE"
     gene            1475..2602
                     /gene="hpnH"
                     /locus_tag="VII37_06765"
     CDS             1475..2602
                     /gene="hpnH"
                     /locus_tag="VII37_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY5069469.1"
                     /translation="MPVPISQMWTVASYVLKQKIRGRKRYPFVLMLEPLFRCNLACAG
                     CGKIQYPAHILKKELTPEECFKAVDECGTPMVSIPGGEPLMHSQIDKIVEGLVARKKY
                     IYLCTNALLLKEKLHLFKPSKYLTFSVHVDGEREHHDFSVCREGGYDLAIEGVREAVK
                     QGFRVTTNTTLFDGADPNSVRAFFDEMMEIGVEGMMLSPGYSYEKAPDQRHFLGRART
                     RRLFRAILSNRKSTWEFNQSPLFLEFLMGKRTYACTPWGMPTYNVFGWQKPCYLLQDG
                     YADSFQELLEATEWQNYGTESGNPKCANCMVHSGYEASAMDHTFSGLGGIWAAAKAML
                     SGKYEDPDALALLNEPTKPVHAYNPLVQIDSSKGQLTETRV"
     gene            2658..3062
                     /locus_tag="VII37_06770"
     CDS             2658..3062
                     /locus_tag="VII37_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069470.1"
                     /translation="MKTNPDKLEVAPGTVHEGIEGWVPELASDDDIRVALEKAFDYRG
                     DVTVTLKSGAKVEGYIFDRVAGPSLATSFVRLLPKTSNERLKVCYADISALAFTGRDT
                     AAGKSFEAWIRRYWEKKAAGEGNLNLEPEALE"
     gene            3261..3566
                     /locus_tag="VII37_06775"
     CDS             3261..3566
                     /locus_tag="VII37_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069471.1"
                     /translation="MDIQVEPFERQPKEGTVQAMQEGQTIKHDVYGLGIVTASDMDRT
                     SVDFDDHGPKLFVTSIMTAELIGEAPATPPKTKRRRKASTAAKAAKKAAAAAAASHA"
     gene            complement(3633..4556)
                     /gene="lipA"
                     /locus_tag="VII37_06780"
     CDS             complement(3633..4556)
                     /gene="lipA"
                     /locus_tag="VII37_06780"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY5069472.1"
                     /translation="MAAPFNILSNAAPPLPIVPTAQARPDWLRVKFFGGERYQDLKRI
                     MRTLDLHTVCESARCPNMGECWEHGTATFMILGDICTRACGFCAVPSGKPAGPPDENE
                     PLRVAEAVAKMGLRYAVVTSVNRDDQPDGGAQIFARTIEEIRRRVPGCKVEVLIPDFR
                     GNWDALNVVLATRPDVLNHNTETVPRLYRQVRKGALYERSLELLKRAKTAHPEVPTKT
                     GLMLGLGEEKDEVLATMQDLAAQGTNILTLGQYLQPTREHLPVVRYVHPDEFAEFKVW
                     GEAMGFKHVEAGPLVRSSYHAFEQTESAHKA"
     gene            complement(4618..4893)
                     /locus_tag="VII37_06785"
     CDS             complement(4618..4893)
                     /locus_tag="VII37_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069473.1"
                     /translation="MTTWHKRDWKQFYELARRPWQRHRPPRPIYPTGINRVLPAAGYS
                     LSELDDAGLDLDLAESLGLPVDAGRIGAYGPNVATLRDFVRSSRQPL"
     gene            complement(5398..5817)
                     /locus_tag="VII37_06790"
     CDS             complement(5398..5817)
                     /locus_tag="VII37_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY5069474.1"
                     /translation="MKKLYIGNLPFSATEEQLNEWFAQVGVTPSGVNLIRDRFTGQSR
                     GFAFVEVNNDEDADRAVNSLNGQNFGGRNLVVNEARPQAERGSGGGGGRPGGGGGGYG
                     GGRGGHGGGGGGRGGGGGRGGDRGDRGGDRGDRGNRW"
BASE COUNT         1435 a         1790 c         1595 g         1366 t
ORIGIN      
        1 ctttcatact cagcgcgaat ttcttcagcc atcgccgccg gatcaattcc cgtttgcttg
       61 gcatacaact tccaaagttg ggggtgacgc ttgatttttt cgtaaagcag cggctgcgtg
      121 atcgttggat cgtccacttc gctgtggcca tgtcggcgat atccgataat atccaccact
      181 acgtcgctgc caaattttgc gcgatactcc gcggcaatct ttccaacgcg aaccaccgca
      241 tccggatcct cggcgtttac atgaaacacc gggaccgact gttgcttggc gatatccgat
      301 gcgaagcgcg acgactgagt ttgaactggg cgcgtcgtga acccgatcaa attatttaca
      361 acgatgtgaa tcgtgccacc gacggtgtac gcctttaaat cagccagatt caaagtttcg
      421 gcccagatgc cttgcccagc gaacgccgcg tcgccatgca tcacaatcgg cacaatcgct
      481 ttcgtgctat ccccgccgag gcgtgtttgc ttcgcgcgag ctcgtcccat cgctaccgga
      541 tcgacagcct cgaggtggct cggattcgat accaggtgaa tcccgatttg cttgcccgtc
      601 gtggttttga ataccccggt tgcgcccacg tgatacttca cgtcgccggc gccaagaacg
      661 ctgcgcggat ccacatcttc aaatccggaa acaacctggt gagccggctt gcacgcggtg
      721 tgcaccatca cgttcagacg cccgcgatgg ctcatggcaa taacgctctc ctgcgctcca
      781 gtttctgccg aggactccag cagcgcatcc agcagaggaa tcagcgcggt cacgccttcg
      841 agcgaaaacc gtttcgcccc aaggtagcgt gcctgcaaaa cctgctcgaa taagtccgcg
      901 cggaccaagc gctcgagtat ttgccgttga tccactgcag gtgccgggct ttccaggcgc
      961 tcggcaatcc attgccgccg ttcctgctcc ggcaagtgca taaattcggc gccaatggtt
     1021 ccgcaataaa ttgcgcgagc ttcgtcggcg gcggggccgc tcaaatttag ttcggggcgt
     1081 tttaccggtt ccagaaaacc gaggggatcc agattggatt cgtaaaaacc ccaccggcgg
     1141 aataattcca aaacacgctc gcgctctgcc acgcccccgg ctttgctgct gccattgggc
     1201 gtcttttttg tcgatttcga cgactttgtc gcgcgttctt cttcgagaat ggttctgtta
     1261 gccataaccg tgcaatcttc attttgccat agattaaaat tggctgccgg agcgcgtgtt
     1321 cagcggctaa ataattggtt cctcacacaa aagctcctaa gcagaaatac aggtatctca
     1381 ggtacaatag aaagggacaa tcttgcgtca gaactggtcg tttgcagtag cgtagtaaag
     1441 actttattcc tgcagctaag gtgtaagcac aacaatgcca gtacctattt cccagatgtg
     1501 gacggtagcc tcctacgtcc tcaaacagaa aatccgggga cgtaagcgct atccctttgt
     1561 cttgatgttg gaaccgcttt tccgctgcaa tctagcctgt gccggttgcg gcaaaatcca
     1621 atatcccgcc cacattttga aaaaggaatt gacgcccgaa gaatgtttta aggccgtgga
     1681 cgagtgcggt acgccgatgg tttccatccc tggcggcgag ccgctgatgc actcgcaaat
     1741 cgacaaaatc gtcgaaggcc tggtcgcgcg caaaaaatat atttatcttt gcaccaacgc
     1801 actattgctg aaggaaaagc tgcatctttt caagcctagc aaatacctga ctttttcagt
     1861 ccacgtggac ggcgagcgcg agcatcacga tttctcggtg tgccgcgaag gcgggtacga
     1921 cctggccatc gaaggcgtgc gcgaagccgt caaacagggc ttccgcgtga ctaccaacac
     1981 aacgctcttc gacggcgccg accccaacag cgtgcgcgcg tttttcgatg aaatgatgga
     2041 aataggcgtg gaaggcatga tgctttcgcc cggctactcc tatgaaaagg ccccggatca
     2101 aagacatttc cttggccgcg cgcgaactcg ccgattgttc cgagcgattt tgtccaaccg
     2161 caaatccacc tgggaattta atcagtcgcc gctatttttg gaatttctca tgggcaagcg
     2221 cacttacgcc tgcaccccat ggggcatgcc gacatataac gtcttcggct ggcagaaacc
     2281 ttgctacctg ctccaagacg gatacgccga cagcttccag gaattgctgg aagcgacgga
     2341 atggcagaat tacggcaccg aatccggcaa tcccaagtgc gctaattgca tggtgcacag
     2401 cggctacgaa gcatcggcga tggatcacac ctttagcggc ttaggcggca tttgggccgc
     2461 cgcgaaggct atgctgtctg ggaagtacga agacccagac gcgctcgcgc ttctgaacga
     2521 accgactaag ccagtgcacg cttacaaccc tctcgtgcag atcgattcga gcaagggcca
     2581 actaacagaa accagagtct agtggcttag gcgcatctgt aagtttgaaa aacatgtaac
     2641 cggaacctta tccaatcatg aaaactaatc ccgacaaatt agaagtcgct ccaggcacgg
     2701 tgcacgaagg catcgaaggc tgggtccccg aacttgcaag tgacgacgac atccgcgtag
     2761 ccctcgaaaa ggcttttgac taccgcggtg atgttaccgt gacgctgaaa agtggcgcca
     2821 aagtcgaggg atatattttc gaccgcgtag ccggaccttc ccttgccacc agcttcgtcc
     2881 gtctccttcc gaaaacttca aatgaacgcc tcaaagtttg ctatgccgat atatcggcgc
     2941 tagcctttac cggccgagac actgcggcgg gcaaaagctt tgaagcctgg atccgcagat
     3001 attgggagaa aaaggctgct ggcgagggca atttgaacct agagccagag gctttggagt
     3061 aacttcccct ccgcgcctac cacctagcta gcatcgttaa gtgtttataa atattagcgt
     3121 tacagtcaat tcatgattgc ctgcccttcc ctagagcgct agacaattgc tctcttttgt
     3181 agtaatatga ctgagtgatc gagttcgtcc ctcggcccta agcggcttga ggcggcggtt
     3241 tcagcctacc tgtttcaggc gtggatatcc aagtcgagcc attcgaacga caacccaagg
     3301 agggcactgt gcaggcgatg caggaaggtc aaactattaa gcacgacgtt tacggtctgg
     3361 gaattgtgac cgcttcggat atggaccgca cctcggtcga cttcgacgat cacggcccta
     3421 agctctttgt caccagcata atgaccgccg aattgatcgg cgaagcgccc gcaacgcctc
     3481 cgaagaccaa gcgtcgccgc aaagcctcca cagcagcaaa ggccgccaag aaagccgcag
     3541 cagcagcggc agcgagtcac gcataattta gtaactcgat cttctgattg attagttcca
     3601 acgccctcga atgagggcgt ttttgttttc cgtcaagctt tatgcgcgga ttcggtttgt
     3661 tcgaaggcgt gatacgaaga gcgaaccagc ggcccggcct caacatgctt gaaacccatc
     3721 gcctcgcccc atactttaaa ctccgcaaat tcatcgggat gaacgtagcg caccactgga
     3781 agatgttcgc gggtaggctg tagatattgt cccagcgtca aaatattcgt gccctgcgcg
     3841 gccaagtcct gcatggtcgc gaggacctcg tctttttcct cgcccaggcc caacattaat
     3901 ccagtcttgg taggaacttc cggatgcgcg gtcttcgcgc gtttcaataa ctccagcgag
     3961 cgttcgtaga gcgcgccctt ccgaacctgg cgatacaatc gcggaaccgt ttccgtattg
     4021 tgattcagca catcggggcg cgtcgccagc acgacgttga gggcatccca gttgccgcga
     4081 aaatccggaa tcagcacttc cactttacat ccaggcaccc tgcggcgaat ttcttcgatg
     4141 gtgcgagcaa agatctgagc gccgccgtca ggctgatcgt cacgatttac cgaagtcacc
     4201 accgcgtatc gcagacccat tttcgcgacc gcttccgcaa cgcgtagggg ttcgttttca
     4261 tctggaggtc cagcgggctt accggacggc acagcgcaga atccacaggc acgcgtgcaa
     4321 atatctccca ggatcatgaa ggtcgcggtc ccatgttccc agcattcacc catgttggga
     4381 caacgcgcgc tttcgcatac ggtgtgcaga tccagcgtgc gcatgatgcg ctttaagtcc
     4441 tggtagcgct cgccgccaaa aaacttaacg cgcagccaat ccgggcgcgc ctgagccgtc
     4501 ggcactatcg gtagtggcgg agccgcgtta gaaaggatgt taaagggtgc agccattgga
     4561 tgggttcaag ctattttaca cgcggaggcg cgaaagcgcc aaaaaagcca gtgaagttta
     4621 aagaggctgg cgggaagaac ggacgaaatc tcgcagcgtc gcaacgttag gaccgtaagc
     4681 gccaattcgc ccagcgtcca caggtagccc aaggctctcc gccaaatcca gatccaaacc
     4741 cgcatcatcg agttcagaca ggctgtatcc cgccgctggt agaacacgat ttatgcccgt
     4801 cgggtagata ggccgaggcg gacgatgacg ctgccacgga cggcgcgcca gctcgtaaaa
     4861 ttgtttccaa tcgcgtttgt gccaggtggt catttgcttt ggtcgccgct tacaatcggt
     4921 ccttaacctc gctaccacta atgttagtac gtcttgcttc aaacgttgga tgcatgtctc
     4981 gcattttggg ttcgcccccc tgtgtaaaat ccttgtaaag aggagcgacc taaaataaac
     5041 gcgagctact gatatacttc acattctccc caggcaatgc agtttctttc cgcattgttt
     5101 caaacccgtc attactcgat ttgttccgct cgccaagccc tcagcaggag cctgtaggaa
     5161 atgagagaaa cgactgtatg cgggagttaa agccggacaa aaacgaaact cttttaattg
     5221 ttcttcggcg gaaatactaa agaatcagct caaaacgcgc gcggctaggg catcgcatct
     5281 ttgctgagta acttatgaag agaggtagtt ccggacacag gtctcttaaa cgacgccagg
     5341 aaagaactac ttgtggcctt gcaggataga aacgccgaga atttagacag gacccactta
     5401 ccagcgattg ccgcggtcac cacgatcccc gccgcggtcg ccgcgatcac caccccggcc
     5461 gcctccgccg ccgcgtccac caccgccgcc tccgtggccg ccccggccgc cgccgtagcc
     5521 accaccgcca ccacctggcc gccctcctcc accgccgctt ccgcgttcag cttgggggcg
     5581 tgcttcgttg actaccagat tgcggccgcc aaaattctgg ccattgaggg agttcacggc
     5641 gcgatcagca tcttcatcat tatttacttc cacaaatgcg aacccgcgcg attgtccggt
     5701 gaagcggtca cgaattaagt taacacctga gggagttaca ccgacttgag cgaaccactc
     5761 gttgagctgc tcttccgtgg cggaaaacgg aagattaccg atatagagct tcttcacctg
     5821 cgcactcctt ctttcaggaa ccccactgcg agtcacggag cgcactgaag aaacatctgt
     5881 agcagggctg tcgtaagacc gggccaccgc ggacgcagac tgtgacttca gcggtcagtg
     5941 gaatcctctc gaaaaattat cccttttgca accggaatga ttttgttaga gccatctgta
     6001 tctttgctca ttgatggccg aagcaaacat ggaacgtgag cgcgaatctc aaatacatca
     6061 cgtgcgatag ctggcgttaa tgcgcacata ttccgcggtg aaatcgcatg tccaatagcg
     6121 cgccaccgcg ccaccagcac gcatgttcac aatgattggg acgtgtttac cgagcagacg
     6181 gttact
//
LOCUS       DATCBB010000619         1007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_410153, whole genome shotgun sequence.
ACCESSION   DATCBB010000619 DATCBB010000000
VERSION     DATCBB010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410153"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="murD"
                     /locus_tag="VII37_06795"
     CDS             complement(<1..992)
                     /gene="murD"
                     /locus_tag="VII37_06795"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY5069475.1"
                     /translation="MVAGNIGTPLISRVALSSPDTITVAELSSFQLELIETFHADIAV
                     LLNLTPDHLDRHKTMEAYAAAKARLFENQTKMDFAVLNADDPAATRLAPKRPQLYWFS
                     RKREVLQGAYLRGEEIVFRTSKQVEVLLKREEISLPGAHNLENVLAAVTAARLEGAPA
                     SAVREAVRTFPGVEHRLEFVAEIGGVRYYNDSKATNVDAALKALEAFPGRVLIILGGK
                     DKDSDYSVLQPALRERSVLALLIGAAAEKIEQQISGSVALERAGTLERAAEIASQAAR
                     RGDVVLLAPACASFDQFENYEHRGRVFKELVKQQEQKLASLSDEVKSDGKANTSA"
BASE COUNT          210 a          322 c          256 g          219 t
ORIGIN      
        1 gcactcgtat tcgcctttcc atccgatttc acttcgtcgg acaaggaagc aagtttctgt
       61 tcctgttgct tgaccaattc cttaaaaacc cgcccgcgat gctcgtaatt ttcaaactgg
      121 tcgaagctcg cacatgccgg cgcgagcagc acaacatcgc cacgccgcgc agcttgcgag
      181 gcgatttccg ctgcgcgttc tagagtcccg gcgcgctcca atgcgacgct gccggagatt
      241 tgctgttcga ttttttccgc cgctgcgccg atcagcaaag ccagcacgct tctctcgcgc
      301 agcgcgggct gcagcacaga ataatcgctg tccttatctt tgccgccgag gataatcagc
      361 acccttccag gaaatgcttc cagcgccttc aaggccgcat ccacattggt cgctttcgaa
      421 tcgttgtaat agcgaacgcc gccaatttcc gccacaaatt ccaaacggtg ctccactccg
      481 gggaaggtgc gcaccgcctc acggacggcc gacgcaggcg cgccttccag acgcgcggcc
      541 gttacggcgg ccaagacgtt ttcaagattg tgcgcgccgg gcagcgaaat ctcttcacgt
      601 ttcaacaaca cttctacctg cttgctggta cgaaaaacaa tttcctcgcc tcgcaaatac
      661 gcgccctgca acacttcacg tttgcggctg aaccaataga gctgcggccg cttcggcgcc
      721 aggcgcgtcg ccgcaggatc atccgcgttc aaaaccgcga aatccatttt ggtttgattt
      781 tcaaacaagc gggctttcgc cgccgcataa gcttccatgg tcttatggcg gtccagatga
      841 tcgggcgtga gattcagtag caccgcgatg tctgcgtgaa acgtttcaat cagctccagc
      901 tgaaaactgc tgagctccgc gaccgtgatg gtatccgggc ttgaaagcgc cacacgagaa
      961 atcagcggcg tcccaatgtt ccctgcgacc atcgtggaga agcccgc
//
LOCUS       DATCBB010000620         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_246721, whole genome shotgun sequence.
ACCESSION   DATCBB010000620 DATCBB010000000
VERSION     DATCBB010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246721"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VII37_06800"
     CDS             complement(<1..849)
                     /locus_tag="VII37_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY5069476.1"
                     /translation="MTARSDVPMVRESASRSERATVRKPRSLANRSVVTPFAPASPAE
                     TSKIAAGMAELTRLGWKVTERAPTVNQGYFAGTTKTRRDELVGALERNDIDALVGTRG
                     GYGSNYLLDELHIPAAAIPKIILGFSDLTSLQIYLWQRFQWVTFYGPMLAAGLDAGAG
                     APKGYDDKSLLAAIRNTESGWALDLQGKVLNSGEARGLVLGGCLTLVETAIGTAWELE
                     TDGAILVLEDRGMKPWQVDRALMHLMQAGKFKGVHGIMLGDFPECESPVAGGPTVRDV
                     CRRILSP"
     gene            complement(846..1511)
                     /locus_tag="VII37_06805"
     CDS             complement(846..1511)
                     /locus_tag="VII37_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY5069477.1"
                     /translation="MIPLNDIIVRLLWAALLGSLIGAERAFHRRPAGLRTGLCVGVGA
                     AFFTILSIEIARRTGDTSTTRIASNIVQGIGFLGAGVILRDPGGVTGLTTAATIFVVA
                     ALGMGAGAGLFWPSGFACVLVLFALVFLVYVESWFGLKPRFMLFRISTDPTVDLVSEV
                     HQCFSALGITLDNFQVSMAGEKNLIQFDADVSHRKQEKIFAGLTRPGITVEMLPVERQ
                     QQA"
     gene            complement(1555..>2342)
                     /locus_tag="VII37_06810"
     CDS             complement(1555..>2342)
                     /locus_tag="VII37_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5069478.1"
                     /translation="VNLVGIVGDDFTSRDAEIFRGRTIDIEGLERAAGKTFFWSGRYS
                     ENLNERTTLVTDLNVFADFKPKLPEKYRKSKHVFLANIAPDLQRDVLQQVTKRPTIVA
                     LDTMNYWIERTNAELRETLKHVDILMINDFETRQLSSEHNLLRAAKHIFKMGPSTLVV
                     KRGEYGAMMVDKRGVFCVPAFPLEEPHDPTGAGDSFAGGFMGYLAGAGKTNDAALRRA
                     MVYGSVLGSFAVEKFGLERLRSLKRSEIHARARHFAKLTQFSL"
BASE COUNT          560 a          719 c          574 g          489 t
ORIGIN      
        1 cggcgaaaga attcgccggc acacatcgcg aacggttggg ccgccggcaa caggcgattc
       61 gcactccgga aaatcgccta gcatgattcc gtggacaccc ttaaattttc cagcctgcat
      121 cagatgcatc aaggcgcggt caacctgcca cggtttcatg ccgcgatctt ccaggacaag
      181 aatcgcacca tcggtttcca attcccacgc cgttccgatg gccgtttcca caagagtcaa
      241 gcacccaccg agcaccaggc cgcgcgcttc gccggagttc aacactttcc cttgcaggtc
      301 taacgcccag ccgctttccg tgtttcgtat agctgccagc aacgatttgt cgtcatatcc
      361 ctttggtgcg ccggcgccag cgtcaagacc tgcagcgagc atcgggccgt aaaatgtgac
      421 ccattgaaat cgctgccaca aataaatctg caaagaagtc aggtcgctga aacctaaaat
      481 aattttagga atcgcagccg ccggaatatg cagctcgtcc agcagatagt tcgagccgta
      541 gccgccgcgc gttcccacaa gtgcgtcaat atcgttacgt tccaaagcac ctaccagctc
      601 gtcgcgtcgg gtcttcgtcg ttcctgcaaa atatccctga ttcacggtcg gagcgcgttc
      661 cgtgaccttc catcccagac gcgttaactc cgccattccc gcggcaattt tagatgtttc
      721 ggctggcgat gccggagcga acggcgtgac cacgctgcgg ttggctagcg atcgtggctt
      781 acgcaccgtg gcacgttcac tccgcgaagc ggactcgcgc accatgggca cgtcagaacg
      841 agccgtcatg cttgttgctg cctctcgacc ggaagcatct ctacagttat cccgggccgc
      901 gtcaaacccg cgaatatttt ttcctgcttc cgatgactca catccgcatc aaactgaatc
      961 agatttttct ccccggccat ggacacctga aaattatcca gcgtgatacc aagggccgaa
     1021 aaacactggt gaacctcaga aaccagatcg acggttggat cggtggaaat tcgaaacaac
     1081 atgaaacggg gcttcaaacc gaaccaactt tctacgtaca ccagaaatac cagcgcaaaa
     1141 agcaccagca cgcaggcaaa tcccgaaggc caaaacaatc cagcaccagc acccattccg
     1201 agcgcggcga ctacaaaaat tgtggcggcc gtggtcaatc cggtcacgcc gccgggatcc
     1261 cgcaaaataa cgcccgcgcc aaggaaacca attccctgaa cgatgttgga agcaatgcgc
     1321 gtggtgctgg tatcgcctgt gcggcgcgca atttctatgg agagaattgt gaaaaacgcc
     1381 gcgcccacgc ctacacacaa gccggtgcgc aaaccagcag ggcgacgatg gaaggcccgc
     1441 tccgcgccaa ttaacgaacc caacagcgcg gcccacagca agcgcacgat tatgtcgttg
     1501 agagggatca tgcttttcgc cggtgtagcg agcgaactct gaaaagttta cagtttatag
     1561 cgagaattgc gttaatttcg cgaagtgccg tgccctggcg tgtatctcgc tgcgttttag
     1621 cgaccgcaaa cgctccaacc caaatttctc caccgcgaag ctgcccaata ccgagccgta
     1681 taccatggcg cgtcgcagag ccgcatcatt cgtttttccc gcgccggcca gataacccat
     1741 gaatccgccc gcaaaactat ccccagcgcc cgtcggatca tgcggctctt ctaacggaaa
     1801 cgctggaacg caaaataccc cgcgcttgtc gaccatcatg gccccgtatt caccgcgctt
     1861 taccaccaag gtgctgggtc ccatcttgaa aatgtgcttg gccgcacgca gcagattgtg
     1921 ctcgctggac agttggcgcg tttcaaaatc gttgatcatc aaaatatcca catgtttcag
     1981 cgtttcgcgc aactccgcgt tggtccgctc gatccagtaa ttcatggtgt ccaacgccac
     2041 gattgtcggc cgcttggtaa cttgctgcag tacgtcacgc tgcagatcag gagcaatatt
     2101 cgcgaggaag acgtgtttgg atttgcggta tttttccggc agcttgggct tgaagtccgc
     2161 gaaaacattc aaatcggtca ccaacgtggt gcgttcattc aaattttcgg agtagcgccc
     2221 cgaccagaaa aacgtttttc cggcggcacg ctctaatcct tcgatatcta tcgtccgtcc
     2281 acgaaatatt tcggcatcac gcgaagtgaa atcatcacca acgataccca ccaggttcac
     2341 gt
//
LOCUS       DATCBB010000621         1664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_118425, whole genome shotgun sequence.
ACCESSION   DATCBB010000621 DATCBB010000000
VERSION     DATCBB010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118425"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1190)
                     /locus_tag="VII37_06815"
     CDS             complement(3..1190)
                     /locus_tag="VII37_06815"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY5069479.1"
                     /translation="MLSEFNASLLAELQAGLRDLESRSQRRTLVEITGVNLCSNDYLG
                     LADHPGLKQTVIEAVLKANRIGGTGSRLLSGHAAVWNELEEEVAAFAGSAAALYFGSG
                     YAANLGLLTSLANKNDVIFSDALNHASIIDGIRLSGARKIIYPHRDLNALESALKENQ
                     SKPGRKLIVTETVFSMDGDVAPLDAIVALAEKYGASVIVDEAHATAVHGQQGRGIAQQ
                     LLTDGRVLAAMHTCGKALASAGAFVCGSTILREHLINHARTFIFSTAMPPYMAEQIRA
                     ALRLVAGMDIERAELISRSQGFAQSLQRDGWETLTTTTQIVPAVVGPNDAAVAAAEFL
                     QRAGFAVRAIRPPTVPQGTARLRFSVTHKISAAELERLTAVLSTWRAQSHHPSPLALA
                     GSA"
     gene            complement(1180..>1664)
                     /locus_tag="VII37_06820"
     CDS             complement(1180..>1664)
                     /locus_tag="VII37_06820"
                     /inference="COORDINATES: protein motif:HMM:NF018651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069480.1"
                     /translation="EFYGSIITTRVYDDRLRTLASVRKAGITVCCGGILGMGESERDR
                     IGLLHQLVTLEPHPESVPINMLVRVAGTPLADKPALDPLEMVRAIATARVLMPASRVR
                     LAAGRKQLSPEAVTLCFLAGANSIFVGEKLLTTPNPGADEDEQLLQTLGLKPLESHAI
                     "
BASE COUNT          337 a          460 c          499 g          368 t
ORIGIN      
        1 cctcacgcgg agcccgccaa tgccagaggg ctgggatgat gcgattgcgc gcgccacgtg
       61 gataaaactg cggtgagcct ttcaagttca gctgcactga ttttgtgcgt gaccgaaaag
      121 cgcagccgcg cggtgccttg cggcactgtg ggcgggcgga tagcccgtac ggcaaatccg
      181 gcgcgctgca aaaattcggc ggcggctacg gcagcatcgt ttggaccaac gaccgctgga
      241 acaatttgtg tagtagtggt gagcgtttcc cagccgtcgc gctggagtga ttgcgcgaag
      301 ccttgcgacc ttgaaattaa ttccgctcgc tcgatgtcca ttccggcgac cagacgcaga
      361 gctgcgcgaa tttgttcggc catgtagggg ggcatggcgg tgctaaaaat aaatgtgcgt
      421 gcgtgattga ttaaatgctc ccgcaatata gttgatccac aaacgaatgc gccagcgctt
      481 gccaaagctt tgccgcaagt gtgcatggcg gcgaggactc gtccgtcggt caggagttgc
      541 tgggcgattc cgcgtccttg ctgtccgtgc accgccgtgg catgggcttc atcgacaatc
      601 acgctggcgc cgtacttctc tgccagcgcg acgatagcgt cgagtggggc gacgtccccg
      661 tccatgctga agaccgtttc ggtgacgatc agcttacgtc caggcttcga ctgattttct
      721 ttcagggccg actccaacgc gtttagatca cgatggggat aaattatttt tcgcgcgccc
      781 gacaagcgaa ttccatcgat gatgctggcg tgattaaggg catcggaaaa aatcacatcg
      841 ttcttgtttg ccaacgacgt cagtaagccg aggtttgcgg cgtaaccgct gccaaaatat
      901 aaagccgcgg cgctacctgc gaacgcggcg acctcttctt ccagctcatt ccaaactgca
      961 gcgtgcccgg agagcaggcg cgagcccgtg ccgccaatcc gattggcctt gagcacggct
     1021 tcgatcacgg tttgtttcag cccgggatgg tccgccaatc caagataatc attcgagcat
     1081 agatttacgc cggtgatttc gacgagcgtc cggcgctgac tccgcgattc aagatcgcgc
     1141 aggccggctt gtaattcggc taggagtgat gcgttaaatt cagatagcat gtgattcgag
     1201 tggtttcaat cccaaggttt gcagtaactg ttcgtcctca tccgcgccgg ggttgggagt
     1261 tgttagtaat ttttcgccga cgaaaatcga atttgcgcct gcgagaaaac atagcgtcac
     1321 ggcttcaggc gacaattgtt ttctacccgc ggcgagacgc acgcgcgaag cgggcatcag
     1381 cacgcgtgcc gtggctatcg cgcgcaccat ttcgagcgga tcgagcgccg gtttatccgc
     1441 gagcggtgtg cctgcgacgc gcaccagcat gttgatgggt acactttccg ggtgcggctc
     1501 gagagtgacg agctggtgca acaagccgat gcggtctctt tcgctttcgc ccatgcccaa
     1561 aattccgccg caacacacgg tgatgcctgc tttgcgcacg gaggctagcg tccgcaagcg
     1621 gtcgtcgtag acgcgagtgg taatgatcga gccgtaaaat tcag
//
LOCUS       DATCBB010000622         1016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_475563, whole genome shotgun sequence.
ACCESSION   DATCBB010000622 DATCBB010000000
VERSION     DATCBB010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475563"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VII37_06825"
     CDS             <1..265
                     /locus_tag="VII37_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069481.1"
                     /translation="GRWFYCAEEVRVCRHFDECICNRRPWSAKKGLKSNSLGGAGKRH
                     SLGAGKFDRATATSGDQLSNLALTTRAAIRGDRSAAVTRLIFF"
     gene            520..750
                     /locus_tag="VII37_06830"
     CDS             520..750
                     /locus_tag="VII37_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069482.1"
                     /translation="MAYAPSAVAPRDLAPTHAVARNGRGLLLRFFDAMMASRQRQVDR
                     EIARFIHDNGGKFTDNTEREIERRFLSGPSRW"
BASE COUNT          218 a          282 c          301 g          215 t
ORIGIN      
        1 agggcggtgg ttctattgcg cggaggaagt gcgagtgtgc aggcattttg acgaatgcat
       61 ttgtaatcgt cgaccatggt cagcgaagaa agggctcaag agcaattccc ttggtggggc
      121 gggaaagcgg cattccctcg gagccggcaa gttcgatcga gcgaccgcga ccagcgggga
      181 ccagctaagc aacttggcgc tcacaactcg ggccgcaatc cgcggcgacc gcagcgccgc
      241 cgtcacacgc ttgattttct tttaatgttc tgcggcggaa aagcggcggg actgcatcgc
      301 tgccgtcaca atgacgacgc gataagcgat ccgcgccgca tttggaatca aatgcacaca
      361 attgcgacaa tggcgcaacg caacgcatga gtcatgcgca tggaccgcat gctatccggc
      421 cacttctgcc actggcgaat ccgcgtatat tgcttagcaa caacaaaaag gaggtacggg
      481 aaacgccttg gatcgtcgac ctttgaacgg agatgaacca tggcttacgc cccgtccgcg
      541 gttgccccgc gtgacctcgc tcccacccac gcagtcgccc gcaacggccg cgggctgttg
      601 ctgcgctttt tcgacgccat gatggcgtcg cgccagcgcc aggttgatcg agagattgcg
      661 cgctttatcc acgataacgg cggcaaattc accgataata ccgaacgtga gatcgagcgt
      721 cgttttctgt ccgggccgtc gcgctggtga aatgatttcg gtgctggcgt tttcgcccac
      781 gagcgcatga ttccgaaagg tgagagccgg tttttggaaa cggccatgcg cacagacaat
      841 attaaggcgg cgttctgatt aaatcagagc cgatcagact caaaggagaa ttgaaatatg
      901 ttgacgttat cgattcgcgc tccgcatgtg ccgtatggat ggttgctccc cggatttgcg
      961 cgcatcgttt cctttgtcga ggccgtactt gacgtgcttg cctatgcgga ccggca
//
LOCUS       DATCBB010000623         1447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_344687, whole genome shotgun sequence.
ACCESSION   DATCBB010000623 DATCBB010000000
VERSION     DATCBB010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344687"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VII37_06835"
     CDS             <1..243
                     /locus_tag="VII37_06835"
                     /inference="COORDINATES: protein motif:HMM:NF023190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEY5069483.1"
                     /translation="PVARAVWRAEPDLKVAATTWILAGGSHHTALSKALTAEHVQDFA
                     DIAGLECLRIDANTNVRTFSNELKWNDLYYHLAKAV"
     gene            259..>1447
                     /locus_tag="VII37_06840"
     CDS             259..>1447
                     /locus_tag="VII37_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY5069484.1"
                     /translation="MSGVLAALITATRLVRLSPIDLVIVVFYFVLVLAIGFYLKSRSN
                     TAEDFFLAGREMTAWIAGLSFLSANLGALELMGWAAAAYQYGILAAHWYWIGAIPAML
                     FLGIVMMPFYYISKTHSVPGYLKLRFGEASRALSAISFALMTVFMSGINMYSMALVMK
                     VVLGWDINFSIWVSSLTVAVYVTLGGLRSAIFNEVLQFILIWAGALLIPILGLVEAGG
                     WRNLQAQVTANASAEYTHLWSTLGSFSDNPMGIHWTGIVFGLGFIISFGYWTTDFLVV
                     QRVLSAKDLRSAKMAPIIGAAFKMFVPFIVILPGLLALAVLPVKLTGESVAVATGGHS
                     YNEVLPLMLARYCGPGLLGLGITALIAGFMSGMAGNVSAFSTVWTYDIYGALIKKNAS
                     DKHY"
BASE COUNT          267 a          369 c          439 g          372 t
ORIGIN      
        1 ccggtcgcca gagccgtctg gcgcgccgag cctgatctta aagttgcggc gaccacgtgg
       61 attctcgcgg gcgggtcaca tcacacggca cttagtaagg ccttgaccgc ggaacatgtt
      121 caagatttcg cggatatcgc cgggctggag tgcttgcgca tcgatgcgaa cactaacgtg
      181 cgaactttct cgaacgaact caaatggaat gatctttact atcacttggc aaaggccgtc
      241 taaaccaagg agcgatcgat gagcggtgtt ctagcggcgc taatcacggc aacacggctg
      301 gtaagacttt cgccgatcga cttggtcatc gtggtctttt atttcgttct ggtgctggcg
      361 atcggttttt atctgaagag ccgctccaac accgccgaag attttttcct ggcggggcgc
      421 gaaatgaccg catggatcgc ggggttaagt ttcctttccg cgaatctggg cgcgctggag
      481 ctgatgggct gggcggcagc ggcttaccag tatgggattc tggccgcgca ctggtactgg
      541 atcggcgcga tccccgcgat gcttttcctg ggcattgtga tgatgccgtt ttattacatc
      601 tccaaaacgc attcggtgcc gggttatttg aagctgcgct ttggcgaagc ttcgcgcgcg
      661 ctgtcggcga tttcgtttgc gctgatgacg gtgttcatga gcggcatcaa catgtactcc
      721 atggcgctgg tgatgaaagt ggtgctgggc tgggacatca atttcagcat atgggtatcc
      781 tcacttacgg tggcggtgta cgtcacgctt ggcggcctgc gctcggctat tttcaatgaa
      841 gtgctgcagt tcatcctgat ctgggccggc gctttgctga ttcccattct tggcttggtg
      901 gaagctggcg gctggcgaaa tcttcaagcg caggttaccg ccaatgcttc cgcggaatat
      961 acgcacctgt ggtccacgct gggttcattc agcgataacc cgatgggcat tcactggaca
     1021 gggattgtct tcggactggg cttcatcatt tctttcggat actggacaac ggattttctg
     1081 gttgtgcaac gcgtgctttc ggccaaagat ttgcgctccg cgaaaatggc tccgatcatc
     1141 ggtgcggcgt tcaagatgtt tgtgccgttc atcgtgattt tgccgggctt gctggcgctg
     1201 gcggtgcttc cggtgaagtt gactggcgaa tcggttgcgg ttgccaccgg ggggcacagc
     1261 tacaacgaag tgttgccatt gatgctggcg cgctattgcg gacccggatt gctgggactg
     1321 ggaatcaccg cgctcattgc aggctttatg tccgggatgg ccggcaatgt cagcgctttt
     1381 tccacggtat ggacctatga catctacggc gcgctgatca aaaagaacgc cagcgataaa
     1441 cattacg
//
LOCUS       DATCBB010000624         4311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_150704, whole genome shotgun sequence.
ACCESSION   DATCBB010000624 DATCBB010000000
VERSION     DATCBB010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150704"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VII37_06845"
     CDS             complement(<1..642)
                     /locus_tag="VII37_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY5069485.1"
                     /translation="MSNAHEEEALGKAYDSRLMRRLLAYMRPYKWRVILALTLTLAVT
                     PLELAPPKLFQVSVDRYLVPASHQAINVATARHGLVLISLLYLLVLFFDFLAQYVQIR
                     IMQRVGQQTMYDMRQEIFAHIQRLSMSYYDKNPVGRLVTRVTTDVDALNDLFAAGVVT
                     MINDFFLLAVMVGVLLYLNWRLALAALAVLPLILAVTFVFRRFVREANRRIRTA"
     gene            complement(639..2663)
                     /locus_tag="VII37_06850"
     CDS             complement(639..2663)
                     /locus_tag="VII37_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5069486.1"
                     /translation="MSAAATTSATLENKAATAKQPPKVSAWKNLRGLFPYLRRYKGGI
                     ALGLFALALMGVVGNVVPLATGIIIDVLAGSARPFSNGATGVGALTGSWLSRIVPFYA
                     PHSRHTLGIYCLLLVGFIALKGILSFSTRWILIGVSRDIEFDIRNDLLDRLLVLEPEF
                     YVRNRTGELMSRATNDLNAVRMVLGPGIMYSATTLVTMLMAVFLMFKLSPSLTLWVLL
                     PVPIVALVVRHFGQVIHELYEKIQASLAALSVKVQENLSGVRVIRAYAQEDAQIRAFD
                     EPNREYVARNVKLIRTWSMFMPSLQALIGTTFLIVLWKGGFLFLRNQLSLGGLVSFSN
                     FLTLLVWPMIALGWVTNIFQRGAASMGRLTYILNAQPNIDDRGAKIPAGKELQGEIEF
                     RDLTFTYPTMLSGDGNAGVAPSNGTSANKASSSRSITGNGTGPSANTNSSHPILSNIN
                     LRIPAGSTLAIIGPTGSGKTTLAALVARLWDAPNGELLIDGRPIREWPLETLRRAIGY
                     VPQDTYLFGDTLGENIAFGLPQYDAERVREAAEVASLEGDIEDFTEKYETVVGERGIT
                     LSGGQKQRTAIARALVRDPRILILDDSLSAVDTQTEERILSRLRGIMAGRTTILISHR
                     TSTVQGADQIVVLRDGQIIERGKHEDLLARGGYYADLYQKQLLEEELERA"
     gene            complement(2660..3307)
                     /locus_tag="VII37_06855"
     CDS             complement(2660..3307)
                     /locus_tag="VII37_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069487.1"
                     /translation="MEYRVFRTRLVKDFTQNIFIVALTMAAFGSLGVGCGTEAPKPSS
                     EPAAQAKPAEPAIPDDIQDAAKHLLGSDAQVLLVGDLAKNGKRQFLAANVVPKTPKNN
                     VPGTVVTRAVVAQDDDGKWTELLRCDEHLKNQKGYLGLTPLDSINGWRLQFEQIAEKG
                     LQLYFTPLKVNGDTHVLPVGVRWNPATNRYQSLDASYEHFLKESTSLQNVRSTLR"
     gene            complement(3442..4122)
                     /locus_tag="VII37_06860"
     CDS             complement(3442..4122)
                     /locus_tag="VII37_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069488.1"
                     /translation="MANRAYLRVWTRDFSEELMIAEFARFLASAPLSASMNTFAELTV
                     QPVDATETPVASWDLRGQGYGPAEVAALSLQHLNTDSAYVATAKWDLWELNVESLKWQ
                     HAPQPLVLTCNGMDYDNGVAATEGHFVADLGFEHFFTGHAGLLAPGKATNPFDSSDHP
                     VEHTFRRWMAAGGNLKEYHAKTRENIQHLFKWVEALQSALPVERSELWSEGEENFEAR
                     LDAILAQR"
BASE COUNT          996 a         1289 c         1094 g          932 t
ORIGIN      
        1 ggcggttcga atgcgtcgat tggcctcgcg cacaaatctt cggaacacaa aggtgaccgc
       61 gaggatgagc ggcaacactg ccaatgctgc gagtgccagg cgccagttca ggtacagcag
      121 tactccgacc atcaccgcca acaggaaaaa atcgttgatc atggtgacca cgccggccgc
      181 gaatagatcg ttgagcgcat ccacgtcggt cgtcacgcgc gtaaccaacc ggcctaccgg
      241 gtttttgtcg taataactca tcgacagccg ctggatatgc gcaaaaatct cctgccgcat
      301 gtcgtacatg gtttgctgcc caacgcgctg catgatgcga atctgcacgt actgcgccag
      361 aaaatcgaaa aataaaacca gcaaatagag caggctgatc agcacgagcc catgtctggc
      421 cgtcgccacg ttgatcgcct gatgcgaagc aggcaccagg tagcggtcca cgctcacctg
      481 aaacaatttc ggcggtgcca attccagcgg agtgaccgcc agcgtcagcg tcaatgccag
      541 aatcacacgc catttgtacg gacgcatgta tgccagcaaa cgcctcatca accgcgagtc
      601 gtatgctttt ccgagtgctt cttcttcgtg agcgttactc atgcccgttc aagttcttct
      661 tccagcaatt gtttctgata aagatccgcg taatatccgc ctcgcgccag caagtcttca
      721 tgctttccgc gttcgataat ttgcccgtcg cgcaaaacca caatctgatc ggcgccctgc
      781 accgtggaag tgcgatgcga aatcaaaatg gtcgttcgcc cggccatgat cccacgcagg
      841 cgggacagaa tgcgctcctc ggtctgggta tccacggccg acagagaatc atcgagaatc
      901 agtattctgg gatcccgtac cagagcgcgt gcgatcgcgg tccgttgttt ttgtccgcca
      961 gacaacgtga ttccgcgttc accaacgacc gtctcgtatt tctcggtgaa atcttcaatg
     1021 tcgccctcga ggctcgcaac ttccgcggct tcgcgcactc gctcggcgtc gtattgcggc
     1081 aatccaaagg cgatattttc acctagcgta tcgccaaaca aataggtatc ttgcggcacg
     1141 tagccaatgg cacggcgcaa agtttccaga ggccactcac gtatcggacg cccatcgatc
     1201 agcagttcac cgttaggcgc atcccaaagg cgagccacca gcgcagccag cgtggtcttg
     1261 ccgctacccg tcggaccaat aatcgccagc gtggaacctg caggaatcct cagatttata
     1321 ttgctaagta tcgggtggct cgaattggtg tttgcgctgg ggccggtgcc gttaccagta
     1381 atagatctgc tggaagacgc cttgttcgca gacgttccgt tgctgggcgc aacacccgcg
     1441 tttccatctc cggataacat cgtcggatag gtaaacgtca aatcgcgaaa ctcaatttct
     1501 ccctgcaatt ccttgccggc cggaattttt gcgccgcggt cgtcaatgtt cggctgcgca
     1561 ttcaagatgt acgtcaaccg gcccatggat gccgcgccgc gttgaaaaat attcgttacc
     1621 caccccaacg caatcatggg ccacaccagc aaagtcaaga aattcgaaaa tgaaaccaaa
     1681 ccgccgagag aaagctgatt gcgcagaaat aagaagccgc ccttccaaag cacaatcagg
     1741 aatgtggtcc caataagcgc ctgcaatgac ggcatgaaca tgctccaggt gcgaatcaac
     1801 ttcacgttgc gcgccacata ctcgcggttc ggttcgtcga aagcccgaat ctgcgcgtct
     1861 tcctgcgcat aagcccggat cacccgcacg cccgatagat tttcctgtac cttgacggac
     1921 agcgcggcca gtgaggcctg aatcttttcg tacaactcat gaattacttg gccaaagtga
     1981 cgcactacca gcgccacgat cggcaccggc agcaaaaccc acagcgtcaa agaaggcgac
     2041 agtttaaaca tcaaaaagac ggccattaac atggtgacca gcgtcgttgc gctgtacatg
     2101 attccgggtc ccaacaccat gcgcacggcg ttcaaatcgt ttgtagcccg cgacatcagc
     2161 tcgccagtgc ggttgcgcac ataaaattcc ggctccagaa ccagcaagcg gtcgagcaga
     2221 tcattgcgaa tatcgaactc aatatcccgg gatacgccga ttaaaatcca gcgagtggaa
     2281 aatgacaaga tcccttttag cgcgatgaat ccaaccaata gcaggcagta aattcccagc
     2341 gtgtgcctgc tgtgcggagc gtagaaggga actattcggc tcagccaact tcccgtaagg
     2401 gcgccaactc ccgtcgcgcc attggaaaac ggccgcgcgc taccggccag cacatcgata
     2461 ataattccgg tcgccagcgg cacgacgttg cccacaactc ccatcagcgc tagcgcgaac
     2521 aagcccagcg ctatgccacc tttgtagcgc cgcaaataag gaaagagccc gcgcagattc
     2581 ttccacgcgg aaaccttggg cggctgcttt gcagtggccg ccttgttttc caatgtggca
     2641 ctcgttgtgg cagccgcact catcgcagcg tggacctcac attttgcagc gacgttgatt
     2701 ctttcaggaa atgctcataa ctggcgtcca gcgactgata acggttggtg gccgggttcc
     2761 agcgcactcc caccggcagt acgtgtgtat ctccatttac ttttaatgga gtgaaataca
     2821 gttgtagccc cttctccgca atttgttcga attgcaaccg ccatccattg atcgaatcga
     2881 gcggcgtcaa acccaaatac cctttttggt ttttcaaatg ctcatcgcat cgcagcaatt
     2941 ccgtccactt cccgtcatcg tcttgggcca cgacagcgcg tgtgaccacc gtgccgggca
     3001 cattgttctt cggcgttttg ggcaccacat tagccgcgag gaactgccgt ttcccatttt
     3061 ttgccaggtc cccgaccagc agtacttgcg catcgctgcc aagcaaatgt ttcgccgcgt
     3121 cctggatatc gtcaggaatc gccggctccg ctggcttcgc ttgagcagcc ggctctgaac
     3181 ttggtttcgg agcctcggta ccgcatccaa cgcccaaact tccaaacgcc gccatcgtca
     3241 gcgcgacgat aaaaatattt tgcgtgaaat ctttaaccaa ccgcgttctg aacactcggt
     3301 attccattaa agtaatcggc cttttccgcc gggttcggca cgtgaccgcc ctcaacttct
     3361 gaacaaactg gacggttcat ttattgctca ttctagctgc tgtgcgctaa agactctacg
     3421 acttgcttga aacagcgtgt gtcagcgttg cgcgagaatt gcgtccagtc gagcctcgaa
     3481 attctcctcg ccctcggacc acaattcgct gcgctccact ggcaacgcgc tctgcagggc
     3541 ttccacccac ttgaataagt gttggatgtt ctcccgcgtt tttgcgtgat attccttcaa
     3601 attgccgcct gcggccatcc agcggcgaaa ggtatgttcc accggatgat cggaagaatc
     3661 aaacggattg gtggccttgc ccggtgcaag caaaccggca tgcccggtaa aaaaatgttc
     3721 gaatcccagg tcggcaacga agtgcccttc agtcgcggcc acgccattgt cgtaatccat
     3781 cccattgcat gtgagcacca gcggctgagg agcgtgctgc cactttaaac tctccacgtt
     3841 cagctcccac aagtcccact tcgcggtggc aacatacgcg ctatctgtat tcaagtgctg
     3901 cagcgacaac gccgcaactt ctgccggccc atatccctgg ccgcgcaggt cccacgaagc
     3961 aaccggagtt tccgtggcgt ccactggttg gacggttaat tctgcgaaag tattcatgga
     4021 ggccgatagc ggagcgctag ccagaaatcg cgcgaactca gcaatcatca gttcttcaga
     4081 gaaatcgcgc gtccagacgc gcagatatgc acgattagcc atgtatggag gagcttctac
     4141 attataccgg ggacgcacga accatgcgaa ttcgcgctaa cgcgaggagg ggccgccggt
     4201 gcccggactt gctgggatcc ccccttagga aaatgatggg agcaactctg gacggctaac
     4261 tgctgctggt ggtcgctgaa cgcgtgcggc tgttaggatc ggtagataag g
//
LOCUS       DATCBB010000625         1966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_20658, whole genome shotgun sequence.
ACCESSION   DATCBB010000625 DATCBB010000000
VERSION     DATCBB010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20658"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1033)
                     /locus_tag="VII37_06865"
     CDS             complement(65..1033)
                     /locus_tag="VII37_06865"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY5069489.1"
                     /translation="MSNSKSTKRALVTGGAGFLGSHLCDGLLGEGYSVVVADNLLTGS
                     LANIEHLRNDARFEFAEKDVCQPGDWDKLDYVFHFASPASPVDYAEHGIETLRVGSYG
                     TFEALEAARRSGAKFMMASTSECYGDPLVHPQTETYWGNVNPIGPRSVYDEAKRFSEA
                     VTMAYHRYHQVDTRILRIFNTYGPRMQINDGRVIPNFMWQALRGENLTVYGDGSQTRS
                     FCYVSDEVDGILRLARSTEHEPVNIGNPTEFTILECARKVIATTGSASQIKYEALPQD
                     DPKQRRPDIAKAKRLLGWEPKVDLEKGLKLSLEYFRRAVAEKERVA"
     gene            complement(1036..>1966)
                     /locus_tag="VII37_06870"
     CDS             complement(1036..>1966)
                     /locus_tag="VII37_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY5069490.1"
                     /translation="FLYPDRIVVGVESEQAADVMRELYGPLTSGWYYGRADAIPRPEA
                     AKIPPRLIITSTKSAEMIKHASNAFLAMKISFINAVASICESVGANVEQVKDGIGTDS
                     RIGSRFMSPGIGYGGSCFPKDLMAFRAVAKESGYDFRLLEEVIRINEEQRHRFLRKVR
                     SVLWTFRGKRIGVLGLAFKGGTDDIRESPAMLLVQELLREGCHICAYDPAAMERAKEV
                     LKSGVEFASDAYQAAKGADALLILTEWEEFAALDLARLRTLVKYPIVLDGRNLYDPAV
                     MVEHGFSYYSVGRAAALAEESATTSISKKRERA"
BASE COUNT          398 a          623 c          503 g          442 t
ORIGIN      
        1 cgctaggacg acttcactgt gacggtcttt tcttgcgtcc cgcggcatcc acgggagacg
       61 cgagctaggc gacccgctct ttttctgcca cggctcggcg aaaatattcc agagaaagtt
      121 tcaggccctt ctccagatca actttcggtt cccatcccag cagacgtttc gcttttgcaa
      181 tgtcgggacg ccgctgcttt ggatcgtcct gcggcagcgc ttcatattta atctggctgg
      241 ctgagcctgt cgtagctata actttccgcg cgcactccag tatcgtgaat tctgttggat
      301 tgcctatgtt tacgggctcg tgttcggtgc ttcgcgccag acgcaaaatt ccatccacct
      361 cgtcgcttac gtaacagaag cttcgcgtct ggctgccatc gccgtagacc gtgagatttt
      421 ctccgcgcaa cgcctgccac atgaaatttg gaatcacccg cccgtcattg atctgcatcc
      481 gcgggccgta ggtgttgaag atgcgcagaa tgcgcgtgtc gacttgatga tagcggtgat
      541 acgccatggt caccgcctcg gaaaagcgct tggcctcgtc atacaccgag cgcggcccga
      601 tcgggttcac attgccccaa taggtctcgg tctgcggatg caccagagga tcgccgtaac
      661 actccgatgt ggatgccatc atgaacttcg caccgcttcg ccgcgccgct tcaagagctt
      721 caaatgttcc ataggatccg acccgcagcg tctcaatgcc gtgttcggca tagtccaccg
      781 ggctcgcggg gctcgcgaag tgaaatacat aatccagttt gtcccagtca ccgggctggc
      841 aaacatcttt ctccgcaaac tcgaagcgcg catcgtttcg tagatgctcg atattggcca
      901 acgatccggt cagcagattg tctgccacga caaccgaata gccttcaccc aacagcccat
      961 cgcacaaatg cgatcccaaa aagcccgctc ctcctgttac cagtgcacgt ttcgtgctct
     1021 tggaattgct cacgatcagg cgcgctccct ttttttcgaa atcgatgtgg tcgcagattc
     1081 ttccgctaag gcggccgcgc gccccacgct gtaataggag aatccgtgtt ccaccatcac
     1141 cgccggatcg tacaggttcc gtccgtcgag cacgatggga tatttcacta gcgtgcgcag
     1201 acgagccaag tcgagcgccg cgaattcttc ccactcggtc aggatcagca gggcgtcggc
     1261 gcctttcgcc gcctggtagg catccgacgc aaattctact ccgctcttca gcacctcttt
     1321 cgcgcgctcc atcgctgcgg gatcgtaggc gcaaatgtgg cagccctccc gcagcagttc
     1381 ctgcaccaga agcatcgcgg gtgattcacg aatatcgtcc gtgcctccct taaacgccag
     1441 gcccagcacg ccgattcgct tgccgcgaaa ggtccacaac acgctccgca ccttgcgcaa
     1501 gaagcggtgc cgctgttcct cgttgatgcg aatgacttct tccagcagcc gaaagtcgta
     1561 cccgctctct tttgcgacgg cacgaaatgc catcagatcc ttcgggaagc aggatcctcc
     1621 atagccgatt cccggactca taaagcgcga accgattcga gaatccgtgc caatcccatc
     1681 cttcacctgc tccacattcg cgcccaccga ctcgcagatc gaagccacgg catttataaa
     1741 ggagattttc atcgcgagaa aagcattgga ggcgtgcttg atcatttccg cgctcttggt
     1801 gctggtaatg atcaggcggg gcggaatctt ggccgcttcc ggacgcggaa tcgcatcggc
     1861 gcggccgtaa taccatccgc tagtcaatgg cccgtaaagc tcgcgcatga catcagccgc
     1921 ttgctcgctt tcgactccga ccacaatgcg gtcgggatag agaaaa
//
LOCUS       DATCBB010000626         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_20723, whole genome shotgun sequence.
ACCESSION   DATCBB010000626 DATCBB010000000
VERSION     DATCBB010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20723"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..>2266)
                     /locus_tag="VII37_06875"
     CDS             complement(249..>2266)
                     /locus_tag="VII37_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEY5069491.1"
                     /translation="FLTQSAAVSVRNPFVSSKLSANYKAAALTVSADLQNSSEHAVRG
                     VLRAEIGDTKVSQPVQLKAGAIKTVRFSPGKFAALKLTRFHLWWPYTMGEPFLYSAKI
                     SFEVAGHVSDTATTNFGVREVTSELTDKGFRLFKINGRKVLIRGAAWAPDLLFRWVPG
                     KLDADLSYARDMGLNTIRLEGRLDREEFYEKTDRLGILVMPGWTCCDAWERWKDWKGD
                     QREIAAASLKSQIHILRQHPSVFVWLNGSDGPPPADVENMYLGILKDLEWPNPTLSSA
                     SETATTVSGGSGVKMTGPYEYVPPVYWLTDKDAGGAHGYNTETSPGPAIPPRESLERF
                     IPKDHLWPIDDVWNFHAGKERFTTINVFADGLNRRYGTAASLDDFERKAQAVTYDGQR
                     AMFEAYARNKYTSTGVIQWMLNNAWPSLIWHLYDYYLVPAGGYYGTKKACEPVHVQYS
                     YDNNSVAVINSTYEPLAGIKVTAKIYNLDASEKGTKEATLDLGPDSSTTAFELPKVDG
                     LSKTYFLRLQLQDHAGKLVSDNFYWLSTKADVLDWKNKQDTVYTPQAEFGDLTGLATL
                     PEVKLDVTSSSNMDAAKNTTHVTVKNLDNSIAFMVHLRATRKGGTDITPILWDDNYFS
                     LLPGEQREVTATYAASALGGDSAVLEVEGFNVAKQTVEADTATGGNH"
BASE COUNT          511 a          747 c          508 g          500 t
ORIGIN      
        1 agcccgatgg aagctgcgcg ggcttaccga gctgggatgc tgcgccaaac gaccatttcg
       61 gaaaaccgct gtttgcagca cccaagtacg gctcgcgcaa tctgcagctc tcggtgcgat
      121 tcacgttcta gaccttcaaa atacgtgaac aaaaacggat tgcacgtgac ggccgtttct
      181 gccgccacgt gtaattccca cacgcaaata gcaccggcat tcttggatcg gcgtaaatcg
      241 atcggggttt agtggttgcc gcccgtggcc gtgtccgctt ccaccgtttg tttcgccacg
      301 ttaaaacctt ctacctcaag cacggcggaa tcgccgccca acgccgaagc cgcataagtc
      361 gcggtgacct cgcgctgttc acccggaagc agcgaaaaat aattatcgtc ccacaggatg
      421 ggagttatat ccgtcccacc tttgcgcgtg gcccgtaggt gcaccataaa cgcgatgcta
      481 ttgtccaaat ttttaacggt gacgtgcgtc gtatttttcg ccgcatccat atttgaagaa
      541 gacgtcacat ccaatttcac ttccggcaga gtagccagcc cagtcagatc cccaaattcc
      601 gcttgcggcg tataaaccgt gtcctgtttg ttcttccaat cgagcacatc cgccttggtg
      661 gaaagccaat aaaaattatc gctcaccagt tttcccgcgt ggtcctgcaa ctgcagccgc
      721 aggaaataag tcttactaag cccgtccacc ttgggcaatt cgaacgccgt agtagaactg
      781 tctggcccca gatcaagcgt cgcctccttc gtgcctttct ccgaagcatc caggttataa
      841 atcttcgccg taaccttgat gcctgccagc ggctcataag tgctgttgat caccgccacc
      901 gagttattgt cgtaagaata ttgcacgtgc accggctcgc acgccttctt cgtcccgtag
      961 tacccgcctg ccggcaccag gtagtagtca tacaaatgcc agatcagcga cggccaggca
     1021 ttattcagca tccactgaat cactccggta gacgtgtact tattcctggc gtaagcctca
     1081 aacatcgctc gctgcccatc gtaagtaacc gcctgagcct tgcgttcgaa atcatccagc
     1141 gaagctgccg ttccataccg ccggttcagc ccatccgcga atacattgat ggtcgtaaat
     1201 cgctctttgc ccgcgtgaaa gttccacacg tcatcaatcg gccatagatg atcctttgga
     1261 atgaaccgct ccagcgactc tcgcggcgga atcgcaggcc ccgggcttgt ctcggtgtta
     1321 tatccgtgcg ccccgcccgc atccttatcc gttaaccaat acaccggcgg aacatattcg
     1381 taaggtcccg tcatcttcac gccagacccg ccgctgaccg tggtagccgt ctcgctagcc
     1441 gaagaaagcg tcggatttgg ccactccaga tccttcagaa tccccagata catattctca
     1501 acgtcagcgg gcggtggccc gtcactccca ttcagccaaa cgaacacgct cggatgctgc
     1561 cgcaaaatgt ggatctggct cttcaacgaa gccgccgcaa tttcgcgctg gtcgcccttc
     1621 cagtccttcc accgttccca agcgtcgcaa caagtccagc ccggcatcac cagaatgcct
     1681 agccgatcgg tcttctcgta aaattcctcc cgatccagcc gcccttccag ccgaatcgta
     1741 ttcagcccca tatcccgtgc ataagacaaa tccgcatcca gttttcccgg cacccaacga
     1801 aataataagt ccggcgccca cgccgctccg cgaatcaaaa ccttccggcc attaatctta
     1861 aacagccgga aacccttgtc ggtcagctcc gacgtaactt cccgtacgcc aaaatttgtc
     1921 gtcgccgtgt cgctcacatg ccccgcaacc tcaaaagaaa tcttcgctga ataaagaaac
     1981 ggctccccca tggtgtacgg ccaccacagg tgaaaccgcg tcaatttcaa cgccgcaaat
     2041 ttcccgggcg agaaccgcac cgtttttatc gcacccgcct tcagttggac aggctggctc
     2101 accttcgtat ccccaatttc cgcccgcagc acgccccgca ccgcatgctc gctgctgttc
     2161 tgcagatccg cactaacagt caacgccgcc gccttataat tcgcactcaa tttcgacgac
     2221 acaaagggat tgcgcactga cactgccgcg ctctgcgtca aaaaca
//
LOCUS       DATCBB010000627         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_279941, whole genome shotgun sequence.
ACCESSION   DATCBB010000627 DATCBB010000000
VERSION     DATCBB010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279941"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VII37_06880"
     CDS             <1..210
                     /locus_tag="VII37_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069492.1"
                     /translation="VFFNQRASRRDHVALFIMREFRQDAQPAPNYEIIAHGFFAPDAL
                     PDGVTRATRARIAEVLGGAAVSEVW"
     gene            complement(424..585)
                     /locus_tag="VII37_06885"
     CDS             complement(424..585)
                     /locus_tag="VII37_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069493.1"
                     /translation="MSDTITIGRDRPEEEMLTYEVSDEALESAAGDESERAGKYSIAW
                     CTFLTGCPI"
     gene            complement(1804..>2002)
                     /locus_tag="VII37_06890"
     CDS             complement(1804..>2002)
                     /locus_tag="VII37_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY5069494.1"
                     /translation="HSSGVEIGDWNNNGQTIPAQADIQSITINTATTPLPAALPLFAS
                     GLGALGLLGWRRKRKAAALAA"
BASE COUNT          447 a          567 c          544 g          444 t
ORIGIN      
        1 gtgttcttca accagcgggc gtcgcggcgc gaccacgtgg cgctgttcat catgcgcgaa
       61 ttccgccagg acgcgcagcc ggcgcccaat tatgaaatca tcgcgcatgg cttctttgcg
      121 cccgacgcgc tgccggacgg cgtcacccgc gccacccgcg cccgcatcgc cgaggtgctc
      181 ggcggcgcgg cggtgagcga ggtgtggtga gggctcggtg tcgtccccgc gaaagcggcc
      241 tcgtgccctc tactttgcca atgtccgctt tgcccccgaa agcggacatt cattgcggca
      301 gacggaatgt ccgctttgtg ccaatcgcgg acaatcgcta aatgaactgc acaaataaga
      361 aagaccgcct cgcggcggtc cttccgaaat ccgctaacgg acagggtgcc aatagtaatc
      421 tggtcaaatt gggcagccag ttaaaaaagt gcaccatgca atcgagtact tccccgctct
      481 ttcgctctcg tcccccgccg cgctctccag cgcttcatcg gagacttcgt aagtgagcat
      541 ctcttcttca ggccggtcgc gtccgatggt gatggtgtca ctcatagcag tgatccttcc
      601 gttggccagt tgccaaacaa aggcccttgc atttttatta tcgtcataat tgagggcgct
      661 gcatagggcc taccggcagc attgacgcca ccaagacggc atagcgaatg tccgctttgg
      721 tccaagagcg gacaagcgcg ggtgcggttg gaatgtccgc tttgtgtcaa tagctgccgt
      781 cagccaagtc tcgcttcttg atctttgagc gcaaatggaa agaccgcctc gcggcggtca
      841 ttctgtttca gtaagaaaca taagcgccct acccgcaccc cagcgccggc accagcgttt
      901 ttatgattac acaattttat tccgatgccc gacctacgga tttcgtgggt cggcactaat
      961 cgtccagcct tgccaaatta tccgatcaat cactatcgga aaacacgaag gacatgtctt
     1021 tcatcttctg cacaggaagg cttttcacat ctaaatcact gtgcatttga agaacgttca
     1081 tactgacact taggacaggg tttatgtttg catctgcttg tctaggcgga aagaaatact
     1141 gcttcgctcc caacccgata acgagaacag cagcaacagc gattacgtgg tgatgtgcac
     1201 gcatgaacgc ctccctttgc cttgggttgg tgcgcgctac tgtggatcag acgcgataag
     1261 tgcccgttaa aaagacctcg gctatttaag taaatactcg cgcggatgcg cggaaacatt
     1321 gcccaatttg cccgtgaaat ggcagcgttc aaggcggcgt gggagcgcag gtagcccgaa
     1381 ttaccgttgt gtgaactagc tcatatttga cggatcaagc cgagactaca gtccccgaga
     1441 attaaagaag ggatgcaggc accttacggg gatgtcgaat gaaaaataag tatgcgccag
     1501 agccgctatt caatccgtta gtaattgtcc tcacgctcgc cgctatcact ctcatggcga
     1561 tcctactcac gacgggctga tgaatagacc gtattccgca agcagcgagg aggcgatgac
     1621 ggattttaag gcgcggtggg gcgcttaatc catgtccgct cttgtccaat aacggacaaa
     1681 atgttgcggt gccgcgaatg tccgctttgt gccattcgcg accaacgcac ttgcagcaac
     1741 cagctcaaaa agaaagaccg ccaaattgcg gcggtctctc cgaaatccta tcaggtgttt
     1801 tgatcaggct gcgagtgcag cagccttccg cttcctgcgc cagccgagca gacccaacgc
     1861 gccgagacca ctcgcgaaga gcgggagggc ggcgggcaac ggagtagtgg cagtgttgat
     1921 ggtgatcgac tggatgtcgg cctgtgcggg gattgtctgc ccattattgt tccaatcacc
     1981 tatctccacg ccggacgaat gg
//
LOCUS       DATCBB010000628         1060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_53380, whole genome shotgun sequence.
ACCESSION   DATCBB010000628 DATCBB010000000
VERSION     DATCBB010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53380"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..>1060)
                     /locus_tag="VII37_06895"
     CDS             complement(236..>1060)
                     /locus_tag="VII37_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069495.1"
                     /translation="WLRGQMARTSRNVNPSFDEPDSFVLDVKTGVVHTNVGDLANFLN
                     DSVTNSPLKNIKLTGDGDQLKVSGTFRKILPIEAIATIGVAPDNRIQMHVTKINVLKL
                     PFKKLLGGLNVTVASLFHPKDIPGIEVKDNDVFLDTAKAVPPPHIRGQLTSIRIVNPD
                     IQQIYGNAEVDVTRVAQWRNFLQLQGGTIDFGKLTMRHVDLIMVDLSDNAWFDLDLTN
                     YQDQLVNGYTRMTPEAGLQIFMPSLDQIPAEKKNHSISMEWLKHRNVPPPADITSR"
BASE COUNT          224 a          303 c          296 g          237 t
ORIGIN      
        1 tgcgaatcaa aatcgatgaa atccactccg ccgaatccgg ccatgctgct ctcctaaacg
       61 cgccgatact tgccaccgcg cactcacgct gcgccaaaat cttcagagac ttgactgagg
      121 gtgggtggaa aagcgcggtc tgtttcctga gacagtgtaa caagccgctt ctggaggcgg
      181 caaccgcgcg ggcgcaaagc gcagcgagcc gttgccttcg gaagaaatgc tcaattcagc
      241 gcgaggtgat atccgcggga ggcggcacgt tgcgatgctt cagccactcc atgctgatgc
      301 tgtgattttt cttttcggcc ggaatctggt ccaggctggg catgaaaatc tgcagcccgg
      361 cctcgggagt catgcgcgtg tagccgttca ccagttgatc ctggtaattg gtcaggtcca
      421 ggtcaaacca ggcgttgtcc gaaagatcga ccatgatcag gtcgacgtgc cgcatggtga
      481 gtttcccgaa atcgatggtg ccgccctgca gctgcagaaa attccgccac tgcgccacgc
      541 gcgtcacgtc cacctccgcg ttgccataaa tctgctgaat gtcgggattg acgatgcgaa
      601 tgctggtcag ttgcccgcga atgtgcggtg gcggcaccgc cttcgcggta tccagaaaca
      661 catcgttgtc tttcacctct atcccgggaa tatctttcgg atgaaacagg ctggcaaccg
      721 tgacattcaa tccaccaagc aactttttaa aaggtaactt cagtacgttg attttcgtga
      781 cgtgcatctg aatgcggttg tcgggcgcca ctccgatggt ggcaatcgcc tcgatgggca
      841 aaattttacg gaacgtgccg ctcaccttga gctggtcgcc gtcgccggta agcttgatat
      901 tcttcagcgg cgaattcgta acgctgtcat tcaggaaatt cgccaagtcc ccgacgttgg
      961 tgtgcaccac gccggttttc acgtccagca cgaacgaatc gggctcatcg aacgagggat
     1021 tcacgttgcg cgaagtgcgc gccatctgtc cgcgcagcca
//
LOCUS       DATCBB010000629         4408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_279972, whole genome shotgun sequence.
ACCESSION   DATCBB010000629 DATCBB010000000
VERSION     DATCBB010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279972"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VII37_06900"
     CDS             <1..502
                     /locus_tag="VII37_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069496.1"
                     /translation="IEEKTGSLRGFKLAYVGDGNNVCNSLIFLAARLGVHLRIATPAG
                     YEPAREVIQEAKRVARETRAKIELFTDPREAVTGAQGVYTDAWTSMGFEAEGDVRESV
                     FRAYQVNAELMSLAAPDALFLHCLPAHRNHEVTSEVLDGTQSVVLDQSENRMYVQKAI
                     LHTLFS"
     gene            651..1871
                     /locus_tag="VII37_06905"
     CDS             651..1871
                     /locus_tag="VII37_06905"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY5069497.1"
                     /translation="MNKEKYKPKKVVLAYSGGLDTSIIIPWLKENYGCEVIAVIGDVG
                     QQEDLEAARKKALATGASTAVVEDLREEFVRDYIWPTLRAGAVYEHTYLLGTSMARPV
                     IAKRQAEVALKLGADGLSHGCTGKGNDQVRFELAYKAIAPNVQIIAPWREWDIDSRED
                     AIAYAQKHNVQVEQSKKNIYSRDRNIWHLSHEGGELEDPANSPNEAMWQWIASPQNAP
                     DKAEEVEIGFEAGSPVSVNGTKLGPVDLLNTLNEIAAKHGVGRTDIVENRLVGMKSRG
                     AYETPGGTLLITAHRELETLTVDRETAHYQQALSLRYAELVYYGLWFSPLRAALDGFF
                     NVSQQNVTGTVGLKLWKGNLNVTKRVSPFSLYRTDLASFTMGRYNPKDAEGFINLFAL
                     PVTVRPKAKAEGGN"
     gene            1931..3340
                     /gene="argH"
                     /locus_tag="VII37_06910"
     CDS             1931..3340
                     /gene="argH"
                     /locus_tag="VII37_06910"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY5069498.1"
                     /translation="MTERKDDRLWGGRFERAPNAGFDAFQGSFAFDRRLLPYELAVDR
                     AWAKALQGVGILTAEEVRQTLAALDRIGERAQKDKAWLDSSDAEDVHHFVEKALVEEL
                     SALGWKLHTGRSRNELVATDFRLFVKDAAAQIKTAVAELVAAFVALAKANLHLPMAGM
                     THMQHAQPILLSHFLLAHGEAFLRDIPRLESAASSADACPMGSGALAGCSFPIDREAI
                     ARELGFSRITANSLDAVGDRDFALDYLFALAGISTHLSRLSEDFILFASQEFGYVVLP
                     DEFSTGSSLMPQKKNPDAWELIRGKTGRITGMLMGLLTTLKGLPSGYQRDLQEDKEAL
                     FAAHDQVAAMLAVATGALSATKFREDKLNCAASNSALLATEAADYLVRRGVPFRQAHD
                     IVGKLLSEAERQNKKWTEFPLSELKKLSPAFGDDFSDGLNVESALAAKSVPGGTARES
                     VRAACEEIENKLTKLGAKP"
     gene            3440..>4408
                     /gene="argJ"
                     /locus_tag="VII37_06915"
     CDS             3440..>4408
                     /gene="argJ"
                     /locus_tag="VII37_06915"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY5069499.1"
                     /translation="MKHALSEASLPHGFRFAATACGLKKTGALDLGLLCSDEPASAAA
                     VFTQNLVVAAPVSISKENLRNSKGRMRGVVVNAGNANCATGTAGHAAAQRTVAEAAKR
                     LGCHPEELFVCSTGVIGVPLPLEKILRALPVIARHRRPSARSFAELSLAICTTDTRPK
                     TAASSFKMSGKRIHFVGCAKGAGMIHPNMATMLAFVITDAAIAPGLLQKTLRDVTSRT
                     FNAISIDGDTSTNDTLLVLANGAAGAPNIKSGSAAHRAFTAALEDVCRSLALQIVADG
                     EGAQRVIEIEVRGAKSETAAKRIAETIATSPLVKTAFAGGDPNWGRI"
BASE COUNT          985 a         1122 c         1332 g          969 t
ORIGIN      
        1 cattgaagaa aagaccggtt cactgcgcgg ttttaagctg gcttatgtcg gcgacggaaa
       61 taatgtgtgc aattcgttga tatttttagc ggcgcggctg ggagttcatt tgcgtattgc
      121 cactcctgcc ggttacgagc cggcgcgcga agtgattcag gaagcgaagc gggtggctcg
      181 agaaacgcgc gcaaaaatcg aattgttcac ggacccgcgc gaggccgtca ccggtgcgca
      241 gggtgtttac accgatgcct ggaccagcat gggctttgaa gcggaagggg atgtgcggga
      301 aagcgtgttt cgggcttatc aggtaaatgc ggagctgatg tcgctagccg cgcccgacgc
      361 attgtttctg cactgccttc cggcgcaccg caatcacgaa gtcacgtcgg aagttcttga
      421 cgggacgcag tccgtggtgc tggaccaatc agaaaatcgt atgtatgtgc agaaagccat
      481 cctgcacaca ttgttcagtt agcgcaggcg gcggttgcgt cacaatttgg ccgagtgaac
      541 acatatttat tagcgatata aagtccgaag tgttagaact tttcacgata ttagtgtgcg
      601 tcgaattccc gcaacaatga ttgccgatta gttttttgga aagaggagtt gtgaataaag
      661 agaaatataa accgaagaag gttgtgctcg cctattccgg agggcttgat acctccatca
      721 ttattccctg gcttaaagag aattatggtt gcgaggtcat agcggtcatt ggagatgtgg
      781 ggcagcagga ggatctggaa gcagctcgca agaaggctct cgccaccggc gcctcgacag
      841 ctgtggttga agatttgcgc gaagaatttg tgcgcgatta catctggccg acattacggg
      901 ccggcgcggt atacgaacat acttatttgc taggtacatc gatggcccgg ccggtgatcg
      961 cgaagcggca ggccgaagtg gcgttaaaac tcggagcgga cggactctcg cacggctgca
     1021 ctggaaaagg gaatgaccag gtgcgttttg agttggcgta caaagcgatt gcgccgaatg
     1081 tgcaaatcat cgctccgtgg cgtgaatggg atatcgactc tcgcgaagac gccatcgcgt
     1141 atgcgcaaaa acacaacgtg caggtggagc agagcaaaaa gaatatttac agccgcgacc
     1201 ggaatatctg gcacttgagc catgagggtg gcgagctgga agatcccgcg aattcgccta
     1261 acgaagccat gtggcagtgg attgcgtctc cgcaaaatgc tcccgacaag gccgaagaag
     1321 tggaaattgg atttgaagcg ggttctcccg tttcggtgaa tggcacgaaa ctcgggccgg
     1381 tggatttgct gaacacgcta aacgaaatcg ccgcgaagca tggcgtgggg cgtactgata
     1441 ttgtcgaaaa tcgcctggtg ggcatgaagt ctcgcggggc gtatgaaacg cctggtggaa
     1501 cactgctgat caccgcgcac agagaattgg agacgcttac ggtcgatcgt gaaacggcgc
     1561 actaccagca agccttatcg ctgcgctatg cggagttggt ttactacggg ctgtggtttt
     1621 cgccgctgcg cgctgcgcta gacgggtttt ttaatgtttc gcagcagaat gtgacgggaa
     1681 cggtgggatt gaaattgtgg aagggcaatc ttaacgtcac caagcgagtt tcgccgtttt
     1741 cgttgtatcg caccgacctg gcgagcttca ccatgggacg ttataacccc aaagatgcag
     1801 aaggcttcat caatcttttt gcattgcccg tcaccgtgag gccaaaagcc aaagcggaag
     1861 gtggaaatta gaaacgtgat ggtgtgtttt caggattcat aatttggcga gcgcaactga
     1921 agcaacaaaa atgaccgaac gcaaagatga ccggctgtgg ggcgggcgct ttgaacgcgc
     1981 gccgaatgcg ggtttcgacg catttcaagg atcgtttgcg tttgaccggc gtttgctgcc
     2041 ttacgaattg gcggtggatc gggcttgggc caaggcgctg cagggcgtgg gaatactaac
     2101 cgctgaggaa gtgcggcaaa cgctcgcagc gctcgaccgc attggggagc gcgcgcaaaa
     2161 agataaggcc tggctcgatt cgtcggatgc tgaagatgtg caccactttg ttgagaaggc
     2221 actggtcgaa gaactgagcg cgttgggatg gaaattgcat accggccgca gtcgaaatga
     2281 gttggtggcc acggattttc gcctgttcgt caaagatgct gcggcgcaaa tcaagacggc
     2341 tgtcgcggag ctggtcgccg cattcgtcgc gttggccaaa gcaaatttgc atctgcccat
     2401 ggccggcatg acgcacatgc agcacgcgca gcctattttg ctttcacatt ttttattggc
     2461 gcatggggaa gcgtttctcc gggacatccc gcgtctggaa agcgcggctt ccagcgcgga
     2521 cgcttgcccg atgggctctg gggcgttggc cggctgctct tttccaattg accgtgaagc
     2581 aatcgcgcgg gagcttggtt tctcgcgtat tacggcgaac agtctggatg ccgttggcga
     2641 ccgcgacttt gcgctggatt atcttttcgc gcttgctgga atttccacgc atctgtcgcg
     2701 gctgtccgaa gattttattt tgtttgcgtc ccaggaattt ggctatgtcg tgctgcccga
     2761 tgaattttct actggcagca gtttgatgcc gcagaaaaaa aaccctgatg cgtgggaact
     2821 catacgcggc aagaccggac gcattaccgg gatgctgatg ggcctgctca caacgctgaa
     2881 gggtctgccg agtggctacc agcgcgatct tcaggaagac aaggaagcgc ttttcgcggc
     2941 tcacgaccag gtcgcggcga tgctggcggt ggcgacgggt gctttgagcg cgacgaagtt
     3001 tcgcgaggac aaattgaatt gtgccgcgtc taacagcgca ttgctggcca cagaagcagc
     3061 ggattacctg gtgcgccgtg gggtcccttt ccggcaagca cacgacattg tcggcaaact
     3121 cttgagcgag gcggaacggc aaaataaaaa atggacggag tttccgttgt ccgagttaaa
     3181 aaaactctcg cccgcctttg gagatgattt ttctgatgga ctaaacgtgg aatccgcctt
     3241 ggctgccaaa tccgtccctg gcggcacggc gcgggaatca gttcgggcag cgtgtgaaga
     3301 aatagaaaac aaattaacta aactgggagc caagccatga cagcaacaat aagcggtatc
     3361 caacaacaat ctgcatttgt ggcggaggat cggaggcctc ttccgggagc ctcgagccca
     3421 cgcagctgat gtacccgcga tgaaacacgc gctttctgaa gcctccttgc cgcacggttt
     3481 tcgtttcgcg gccaccgcat gtggcttgaa aaaaactggc gcgctagacc ttgggctact
     3541 gtgcagcgat gaacccgcct ctgccgcagc ggttttcacg caaaacctgg tagtggccgc
     3601 acccgtaagt atttccaaag aaaatttgcg taactccaag ggccgcatgc gcggcgtggt
     3661 ggtgaacgcc ggcaacgcta attgcgccac cggcaccgcc ggccacgcgg ccgcgcaacg
     3721 caccgtggcc gaagcggcca agcgtttggg ctgccacccg gaagaacttt ttgtgtgctc
     3781 cactggcgtc atcggggtgc cgttgcctct cgaaaaaata ttgcgtgcgc tgccggtgat
     3841 tgcgcgccac cggcgtcctt cggcgcgttc tttcgctgaa ctgtcgctgg cgatctgcac
     3901 taccgatacg cgcccaaaaa cggcggcctc ctctttcaaa atgtccggca aaagaatcca
     3961 ttttgtgggc tgcgcgaagg gtgcgggcat gattcatccc aacatggcca ccatgctggc
     4021 tttcgtaatt accgacgcgg ccattgcacc cggcctcctg cagaaaacgt tgcgcgacgt
     4081 tacctcgcga accttcaatg ctatttctat cgatggagat acttccacca acgacacgct
     4141 gctggtgtta gctaacggtg ccgctggcgc tccaaacatt aaatccggat ccgcggccca
     4201 tcgagctttc accgcggcgc ttgaagatgt gtgccgttcg ctggcgctgc aaattgttgc
     4261 cgacggcgaa ggcgcgcagc gtgttatcga aattgaagtt cgcggcgcaa aaagtgagac
     4321 cgcggcgaaa cgcatcgcgg agactatcgc tacttctccg ctagtgaaga ccgccttcgc
     4381 cgggggcgat ccgaactggg gtcgtatt
//
LOCUS       DATCBB010000630         1007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_441759, whole genome shotgun sequence.
ACCESSION   DATCBB010000630 DATCBB010000000
VERSION     DATCBB010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441759"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>1007
                     /locus_tag="VII37_06920"
     CDS             66..>1007
                     /locus_tag="VII37_06920"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069500.1"
                     /translation="MHAVRKQAISSAFAIVLIGTLALAGHAQQKQKDRDKKDRDQKAG
                     EQKDSSADFSKPAIDPKLYGAMKWRLIGPFRGGRVLAVTGVTSQPNTYYMGAVSGGVW
                     KTLDGGVSWDPLFDKQSVSSIGAIAVSESDPNVVYAGTGEACIRGNISFGDGVYRSND
                     GGKNWTNIGLKDSRHIGKVIVHPTNPDTVFVAALGHAYGANTERGIFRTRDGGKNWEK
                     VLYLDDRTGGIDIVFDPHNPHVLFAAMWEGYRTPWMLNSGGDKDGLYRSNDDGSTWKR
                     VEGHGMPDGPLGRIGVGVSGEDSNVVYALIEAKKGGLY"
BASE COUNT          231 a          268 c          284 g          224 t
ORIGIN      
        1 aaacacttcc gctaaagcgc ccatcgctgt acgatgccca acttgccatt ttccaggagg
       61 tctgcgtgca cgcagttcgg aaacaagcaa tttcttcagc gttcgcaatc gttttaattg
      121 gcacacttgc tctcgccggt cacgcgcaac agaaacagaa ggatcgcgac aagaaagatc
      181 gcgaccaaaa ggccggcgaa caaaaagact cttccgctga tttcagcaaa ccggccatcg
      241 atccaaaact ttatggcgcg atgaaatggc ggctgatcgg cccattccgc ggaggtcgag
      301 tcctggccgt caccggcgtg accagccagc ccaatactta ttacatgggc gcggtgtccg
      361 gcggagtttg gaagacgttg gacggaggcg tgtcctggga tccgctattt gacaagcagt
      421 ctgtatcctc cattggcgcg attgctgttt ccgaatctga tcctaacgtg gtttacgccg
      481 gcaccggcga agcctgcatc cgcggaaata tttcttttgg cgatggcgtt taccgttcga
      541 atgacggtgg taaaaactgg acaaatatcg gcctcaaaga ttcccgccac atcggcaaag
      601 taatcgtgca tcccacaaat ccggacactg ttttcgtggc cgctctgggg catgcctacg
      661 gcgcgaatac cgagcgcgga atttttcgta ctcgcgacgg cggcaagaac tgggaaaaag
      721 ttttatatct tgatgatcgt accggcggta tcgacattgt tttcgatccg cacaatccgc
      781 acgtgctttt cgccgccatg tgggaaggct atcgcacgcc gtggatgctt aatagcggag
      841 gcgacaaaga tggcctttac cgttcgaatg atgatggcag cacctggaag cgtgttgaag
      901 gccacggcat gcctgacggc ccgctgggcc gcatcggtgt gggcgtatcc ggggaagatt
      961 cgaatgttgt ttacgcgttg atcgaagcga agaagggcgg cctgtac
//
LOCUS       DATCBB010000631         1545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_312443, whole genome shotgun sequence.
ACCESSION   DATCBB010000631 DATCBB010000000
VERSION     DATCBB010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312443"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..345)
                     /locus_tag="VII37_06925"
     CDS             complement(13..345)
                     /locus_tag="VII37_06925"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069501.1"
                     /translation="MPNTDKLVVEREKIADYLLNPAHPDNGGKAEFFVKLGFRREEWE
                     VLAAALRALAQTAEITSHAESPHGGKYVIIGQIESPAARPVAVKTIWIVDKGLDGARL
                     VTAYPHKE"
     gene            594..1049
                     /locus_tag="VII37_06930"
     CDS             594..1049
                     /locus_tag="VII37_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069502.1"
                     /translation="MVSTVGFVVINLKALQGRQSLSCGLLFEYRLGAHSENLNHGRAK
                     KHRYSEVSGAVASFGSTERAVPTWFVRTRDAGTAYHFMHDLAGRLASRVQLTRDGHKT
                     HLAAVEEHSRFLSELKTPSIQTEPLLKNQIAWWKLIFLSPLQGLDSFYN"
     gene            complement(1145..>1545)
                     /locus_tag="VII37_06935"
     CDS             complement(1145..>1545)
                     /locus_tag="VII37_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069503.1"
                     /translation="IGSFTNIRGGHVYPDLILTNEPKPIRIFLQDGLNDNRGKRRGTN
                     TYDAKWDWHAQNIKMVAALTTKGYDVNYCWGIGTHSGKQGGAMLPEMLRWLWRDYPRP
                     DDPQDDNNRNLYVPVEKQPKKSPPSRAIGF"
BASE COUNT          321 a          425 c          415 g          384 t
ORIGIN      
        1 tcataatgcc tttcattctt tgtgcggata agccgtgacc aatctagccc catccaatcc
       61 tttgtccaca atccaaatcg ttttcactgc cacaggcctt gccgccggcg attcaatctg
      121 cccaatgata acatattttc caccgtgcgg cgactccgcg tgactggtta tctccgccgt
      181 ctgcgccaag gcccgtaacg ccgccgccaa aacttcccat tcctcacggc ggaatcccag
      241 cttcacgaaa aactccgcct tgccgccatt gtcgggatgc gccgggttga gcaaataatc
      301 cgcgatcttc tcgcgttcca cgaccagctt gtctgtgttg ggcaatttca ttgacggtgt
      361 tgctataacg aaacagaaat ttctgaatgg ctcaattcaa aataaattcc ttctccgctc
      421 cgggaaggtg gccggaggcc ggatgaggtg tcctcgattt gcgcgaagcc agcgagaaac
      481 agaaacgctg gcaggatgcc ttctggaaaa agaaaacggc gtaaagctct ttaaccacga
      541 tgaacatgaa ccggcaagac gccaagccgc ggagtggcga ccgataaaag cccatggttt
      601 caaccgtggg ttttgtcgta ataaatctca aagccctgca agggcggcag agcttgtcgt
      661 gcggtttgct cttcgagtat cgtttgggcg cacattccga aaacttgaat catggccgag
      721 caaaaaaaca ccgttattca gaggtatctg gagcggttgc ttcgttcggt tctacggagc
      781 gggctgttcc gacttggttt gtccgcacac gcgacgctgg caccgcatat cattttatgc
      841 acgacttggc cggacggctg gcaagccgtg tgcaactaac aagggacggc cataagacgc
      901 atctggccgc cgtagaagaa cactccagat ttttgtctga attaaagacc ccgtcaatcc
      961 aaactgagcc actactcaag aatcaaatcg cttggtggaa attgattttt ctttcgccct
     1021 tacagggctt ggattccttt tacaattaac ccagggcggc gcacgtttca cgttgcttgc
     1081 cctgggctat tatctttcgg gcttacagcc ctttgaatcc gtgcctatcg gtgttcatcc
     1141 gtggttaaaa ccctatcgcc cggctcggcg gcgatttttt tggctgcttt tccaccggca
     1201 catacaggtt gcggttgttg tcgtcttgcg ggtcgtcggg gcgggggtaa tcgcgccaga
     1261 gccagcggag catttcgggc agcatcgcgc cgccttgctt gcccgagtgc gtgccgatgc
     1321 cccagcagta attcacgtcg tagcctttcg tggtcagcgc ggcgaccatc ttgatgttct
     1381 gcgcgtgcca gtcccatttc gcgtcgtagg tgttggtgcc gcgccgtttc ccgcgattgt
     1441 cgttcaggcc gtcctgcaaa aagatgcgga tgggtttggg ttcgttggtg aggattaggt
     1501 cgggataaac gtggccgccg cggatgttgg tgaaactgcc gatgg
//
LOCUS       DATCBB010000632         5088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_280035, whole genome shotgun sequence.
ACCESSION   DATCBB010000632 DATCBB010000000
VERSION     DATCBB010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280035"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VII37_06940"
     CDS             complement(<1..582)
                     /locus_tag="VII37_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5069504.1"
                     /translation="MKLTGAEILCESLTRLGVKHIFGYPGGAILPVYDALGKSKLHHV
                     LVRHEQGATHMADGYARASGEVGCAMATSGPGATNMVTGIATAMLDSSPVVCITGQVS
                     SKVLGSDAFQEIDITGITMPITKHNVVVTRAEDIARTVREAFLIAASGRPGPVLVDIT
                     KDAQQASCEFDWDASAPKLPPKRQRINYDQAEFD"
     gene            complement(579..2309)
                     /gene="ilvD"
                     /locus_tag="VII37_06945"
     CDS             complement(579..2309)
                     /gene="ilvD"
                     /locus_tag="VII37_06945"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY5069505.1"
                     /translation="MSESLPRPRSHTLVDGANRSPARAMLRASGLADGDFKKPLIGVA
                     NTWIEIGPCNYHLRELAKHVKDGIRAAGAVPLEFNTVSISDGITMGTEGMRASLISRE
                     VIADSIELVTRGNLFDALIVLVGCDKTIPAGIMALARLNIPGLILYGGSIAPGQFEGH
                     SVTIQDVFEAVGTYGRGEMSEQRLTALENVACPGAGACGGQYTANTMALVCEFLGVAP
                     MGLSSTPATNDAKTESARRAGELVVDLLRRNVLPSQIINKASIENAIAGVAATGGSTN
                     AVLHLLAIANEAKLPLSIDDFDRISSRVPILADLKPGGRFVATDLYAAGGTALVAKRL
                     LEAGLLRGDAITVTGRTLAEEASKAKEAPGQEVVRKLSEPLKPTGGLVILKGNLAPEG
                     CVVKVAGHNIQNFSGTARVFDSEEAAFQGVENRSVKAGDVMVIRYEGPKGGPGMREML
                     AVTAALVGAGLGDSVALLTDGRFSGATRGLMAGHVAPEAANGGPIAAVVEGDTIVFDL
                     PSRTLNLEITSAEMQRRLASWRAPAPRYTSGVMAKYALLVSSSALGAITAVPQILPSA
                     MQAGTNARTL"
     gene            2599..3708
                     /locus_tag="VII37_06950"
     CDS             2599..3708
                     /locus_tag="VII37_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY5069506.1"
                     /translation="MINGTDVFVIGGGPAGLAAAIAARRRGFQVMIADGNKPPIDKPC
                     GEGLMPDSISALHRLGVEIPSGEGYPLAGIKFLEKEKSAIANFPTGQGVGLRRPILHK
                     KMLDRAAALGVQFLWETAVTGIQGGGVNLAGGKFVRANWIIGADGGQSRVRRWAGLES
                     ADKRDSRLAWRAHFAVAPWSEYVEIHWAEKAQAFVTPVSANEVCVVMVSRNGNRDIRL
                     ALNEFPELARRLRDERLSRPARGTITTMHRLKHVSRGNVALIGDASGSVDAITGEGLS
                     LSFHQAVALAEAMEKNCLDLYQKAHRQFARRPTFMARLLLLLDGRTGLRRRTLSVFRN
                     NPEVFARLVSIHVGETSPAHFAATGALLGWRFLAV"
     gene            3727..4356
                     /locus_tag="VII37_06955"
     CDS             3727..4356
                     /locus_tag="VII37_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5069507.1"
                     /translation="MIYMFRNCIAALVLLLGTAAPIRQAAAQAQSTHAAPAATQIVLS
                     VDPAKSTVHWTVESSLHTVHGTFHVKRGTLSVDPATGKTSGEIVVDATSGESGNDSRD
                     RRMHKEILESSQYSEVVFRPDRADGTILAQGNSSLKLHGIFSLHGADHGLDVPAQATL
                     KDDQWKGTATFEVPYIEWGLKNPSNFLLKVKPVVEVQLDLAGSIRGEAR"
     gene            complement(4395..>5088)
                     /locus_tag="VII37_06960"
     CDS             complement(4395..>5088)
                     /locus_tag="VII37_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069508.1"
                     /translation="EPSMGSIRKYDVEGKLRLYIKFVRIHGFEPALRSIRNLELQSRI
                     KQSAKRVQVQGIEPAVRSFRKLDLNNRLKNSAKTVRNVTEPGLARFSKAFGTEVKKRI
                     HSARIGAAPLADSVQRWWIRNFNEETYGKTLIASVLAFGLLLDILFRMNLAQSSQHPG
                     RLIVRFVGILLCGLFLTAGVSILVRARHKIGADTSELLARTLRWLRAPISPIQTRELS
                     SVAGKIQSHRRA"
BASE COUNT         1191 a         1373 c         1397 g         1127 t
ORIGIN      
        1 atcaaattct gcctggtcgt agtttatgcg ctgacgcttt gggggaagtt ttggcgcgga
       61 agcatcccaa tcaaattcgc aggaagcttg ctgcgcatcc ttggtgatgt cgaccaaaac
      121 tggtccgggg cgtccagacg cggcaatcag aaacgcttca cgaacggtgc gcgcaatgtc
      181 ttcggcgcgg gtcaccacca cgttgtgctt ggtaatcggc atggtgatgc cggtgatgtc
      241 gatttcctgg aatgcgtcgg agcccagaac cttgctgctc acttggccgg tgatacaaac
      301 gaccggcgaa gaatcaagca tggccgttgc aataccggta accatatttg tggcaccagg
      361 gccggaagtg gccatcgcac accctacttc gccactggcc cgcgcgtacc cgtcggccat
      421 atgcgtggcc ccttgttcgt ggcgcaccag cacatggtgc aatttcgatt taccaagcgc
      481 gtcgtagacg ggcaaaattg cgccgccggg atagccaaag atgtgcttca cgcccagtct
      541 ggtcagcgat tcgcaaagaa tttccgcgcc agttaatttc ataaggtcct cgcattggtc
      601 ccagcttgca ttgcagaagg cagtatttgc ggaacagcgg tgatcgcgcc taaagccgat
      661 gacgaaacca gcagcgcata cttggccatc acgccagaag tgtagcgcgg cgcaggggcg
      721 cgccaggagg cgagccttcg ctgcatttcc gcactggtaa tttccaggtt taatgtgcgc
      781 gatggcaaat cgaacacaat ggtatcgcct tcaaccacag cggcgatagg accaccgtta
      841 gcagcttcag gagcaacgtg gccagccatg agcccgcgcg tggcgccaga gaaacgccca
      901 tcggttaaca gcgcgacgct gtcgccaagg ccggcaccaa ctaacgccgc agtgaccgcg
      961 agcatctcgc gcattcccgg gccgcctttc ggcccctcat agcgaatcac catgacgtct
     1021 ccagctttga cgctgcgatt ctccaccccc tggaatgccg cttcttcgct atcaaagacg
     1081 cgggctgtgc cgctgaaatt ttgaatgttg tgacccgcga cttttaccac gcaaccttcc
     1141 ggcgcaagat tgcccttcaa gataaccagc cctccggttg gcttaagcgg ctcgcttaac
     1201 ttgcgaacga cttcctgtcc gggcgcttct tttgccttgc tggcttcttc tgcgagggtg
     1261 cggccggtaa cggtgatagc gtccccgcgc aataatccag cttcaagcag gcgctttgcg
     1321 acgagcgcag tgccgcctgc tgcgtaaagg tcggtagcga caaatcttcc cccaggtttt
     1381 agatcggcaa ggatcggaac acgcgaactg atgcgatcga aatcgtcgat agacaaagga
     1441 agttttgctt cgttggcgat agctagcaaa tgcagcactg catttgtaga gccgcctgta
     1501 gccgccacac cggcgatagc gttttcgatg gatgccttgt ttatgatttg cgacggcaac
     1561 acattcctgc gaagaaggtc gacgacaagt tcgccggcgc gacgagcaga ttcagttttt
     1621 gcatcgttcg tcgcgggtgt gctggaaagg cccataggag cgacgccaag aaattcgcac
     1681 accagcgcca tggtgttggc cgtatattgc ccgccgcaag cgcccgctcc cggacacgca
     1741 acgttctcca acgcggtcaa acgctgctcg gacatttccc cgcggccata cgtgcccact
     1801 gcttcgaaga catcttgaat cgtaacggaa tggccttcaa actggcccgg ggcgatggag
     1861 ccgccgtata aaatcaatcc cggaatattc aggcgagcca gcgccataat gccagcagga
     1921 atggttttgt cgcagcccac caacacgatg agagcgtcaa agagattgcc tcgggtaacc
     1981 aactcgatgg aatctgcgat cacttcccgg ctgatgagcg aagcgcgcat gccttcggtg
     2041 cccatggtga tgccgtcgga aatcgagacg gtattgaact ccagcggaac agcaccggcc
     2101 gcgcgaattc cgtccttcac atgttttgcc agctcgcgaa gatgatagtt acacgggcca
     2161 atttcaatcc atgtgttggc cacaccaatc aatggtttct tgaaatctcc atcagccagg
     2221 cccgaagcgc gaagcatcgc gcgcgccggc gaacgattgg cgccatctac aagcgtgtga
     2281 ctacggggtc tggggagcga ctcgctcaat taaaatttct ccgtttactg gtggtcttct
     2341 tatgggctca agtggcacaa ttactttagt cgacttagag accgaccatc tagatgtgcc
     2401 gacaacaaac cagtccgtga aaataagaat gagcccaaag ctcttataga tcaaattaat
     2461 acttttcgct cggcagataa attcatttta tacagaagtt acgaaccatt tacaccaggc
     2521 atcttgtgtc tcccgtcacg catctttaac aataccctcc tggtggaaag ctaaagcgtc
     2581 aaggaaagcg gacgactcat gatcaatggc acggacgtgt tcgtgatcgg aggcggcccc
     2641 gcgggcttag ctgcagcgat cgctgcgcga cgccggggct tccaagtgat gatcgctgac
     2701 ggaaacaaac ccccaattga taagccttgc ggcgaaggtt tgatgccaga ttcgatttca
     2761 gccctacacc gacttggcgt ggagatcccg agtggtgaag ggtatccact cgccggaata
     2821 aaattcctgg aaaaagaaaa gtccgcaatt gcaaatttcc cgactggaca gggcgttgga
     2881 ttacgccgcc ctatactgca caaaaaaatg ctggatcgtg cagctgccct gggcgttcaa
     2941 tttctttggg aaacggcggt gaccggaatc caaggcggcg gtgtcaacct tgccggtgga
     3001 aagttcgtcc gagcgaattg gattattggc gcggacggcg gtcaatcgcg cgtcagacga
     3061 tgggccggct tagagtcagc tgataagcga gactctcgac tggcgtggcg ggcacatttt
     3121 gctgttgcac cctggtcgga atatgtagag attcattggg ctgagaaggc gcaggcgttc
     3181 gttacgcctg tatctgccaa cgaagtatgc gtggtgatgg tatccagaaa cggaaaccgt
     3241 gacatccgac ttgctcttaa cgaatttccg gaactggcta ggcggttgag agatgaacgg
     3301 ctttccagac cggcacgcgg caccattaca accatgcatc gactcaaaca cgtttctcgc
     3361 ggcaacgtcg cgctgattgg cgacgcctcg ggaagcgtgg atgccattac cggcgaggga
     3421 ttgagcctga gttttcatca agccgtggcc ttggccgagg ccatggagaa aaactgcttg
     3481 gatttgtatc aaaaggcgca ccgtcaattc gcgcggcggc ccacgtttat ggctcggctt
     3541 cttctgttgc ttgatgggcg cacgggcctc cgtagaagaa cgctgagtgt ttttagaaat
     3601 aatccagagg tattcgcacg attggtatcc attcacgtag gcgaaacttc gccggcacat
     3661 tttgcggcca cgggcgcctt actgggttgg cgatttctgg cggtataaat caaggatttt
     3721 ccatacatga tctatatgtt caggaattgt attgccgcgc ttgtactgct cctgggcact
     3781 gcagcaccga ttcgtcaagc ggctgctcag gcccagagta cacatgcagc gccggccgcc
     3841 acacaaattg ttttgtccgt tgaccccgcg aaaagtaccg tgcattggac cgtggaatcg
     3901 agtttacata ccgtgcacgg aacgtttcac gtgaagcgcg gaacattatc tgtcgatcct
     3961 gcaacgggga agactagcgg cgagatcgta gtggatgcga cttcgggtga gagcggtaac
     4021 gacagtcgag accggcggat gcacaaagaa attctcgaaa gcagccaata ctccgaagtg
     4081 gtgtttcggc cagatcgcgc cgacggaacc atactggccc agggaaattc gagtttgaag
     4141 cttcacggaa tattttcttt gcacggggct gatcatgggc ttgatgtgcc cgcccaggcg
     4201 acgctaaaag acgatcagtg gaagggtaca gcgacgttcg aagttcctta tatagagtgg
     4261 ggtttgaaaa accctagtaa ttttctgctg aaagttaaac cagtcgtgga agttcaactt
     4321 gatctggccg gctcgatccg cggcgaagca cggtaacaaa cattattcag cggtgtcgaa
     4381 aactactgta gcggtcaggc gcgcctgtga gattggatct tgcctgcgac ggagctcagc
     4441 tcgcgggtct gtatgggaga tataggagcg cgaagccagc gcaacgtcct cgccaacaat
     4501 tcggaagtat cggccccaat cttgtgacgt gctcgcacta agatcgaaac gcccgccgtc
     4561 aaaaaaagac cacacaacag gattcccacg aaacggacaa ttaagcggcc aggatgttgt
     4621 gacgattgag ccagattcat tctgaaaaga atatccagta gcaagccgaa cgccagaaca
     4681 ctggcgatta aagtctttcc gtaggtctct tcgttaaaat tacgtatcca ccaacgctgt
     4741 acggaatctg caagcggagc ggcgccaatt cgagcgctgt gaatcctctt ctttacttca
     4801 gttccaaagg ctttcgaaaa gcgggcgagg cccggttcag taacgttccg gacagttttc
     4861 gcggaattct tcagcctgtt gttaagatcc aattttctaa acgagcgtac cgccggttcg
     4921 atcccctgaa cctgaacacg ttttgcgctt tgttttatgc gggattgcaa ctccaaattt
     4981 ctaatggagc gaagcgccgg ttcaaagcca tgaatccgaa caaacttgat gtacaacctt
     5041 agttttcctt cgacgtcgta tttcctgatg gagcccatcg aaggttca
//
LOCUS       DATCBB010000633         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_247160, whole genome shotgun sequence.
ACCESSION   DATCBB010000633 DATCBB010000000
VERSION     DATCBB010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247160"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VII37_06965"
     CDS             <1..560
                     /locus_tag="VII37_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069509.1"
                     /translation="ARIGVVFCLCALQLVAGCAERKKPTVSWVGAGLSRPIVPATITA
                     PKPRPLPDESVDPVPELQLEIPPPPPLLAVHTSPARPHVAPAPVAENNGENKPEAPVI
                     APQMSDSEASAARQQMTSSINEAEKNLARSQGKKLNPTQSDLATKIKQFIGDAQSAGR
                     EGDWTRARSLATKAQVLSEELASSL"
     gene            637..1764
                     /gene="thiO"
                     /locus_tag="VII37_06970"
     CDS             637..1764
                     /gene="thiO"
                     /locus_tag="VII37_06970"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEY5069510.1"
                     /translation="MKTFDVAIAGGGLIGGTIAFELARAGLHVAVFDRQQPGEGSSWA
                     AAGILSPAPENAGMISTVPLGRASLQLYPEFVASVEESSGQSAGYRARGTLEALFSTD
                     AQEKLSTIIALHHGLGLKAEPVSAEDAREMEAALSPDLEAAVFRPDEACVDNRALTKA
                     VLIAAERSGVKLFSGSNVQSIAKNGGRCAGLIVDGKKIESRWTVIAAGCFSAEIEGAA
                     QYAPVRPAKGQMIALRGKDFSIERVLWSDHVYLVPRNEGRILAGATVEYVGFEKNVTA
                     GGVQKILTAALELAPALADAQIEETWAGLRPDSPDHLPIIGPTDLDGLLIATGHFRSG
                     ILLAPITAQLIREWITMQNVTVEWARFSPTRFGEVARATNS"
     gene            complement(1800..2597)
                     /locus_tag="VII37_06975"
     CDS             complement(1800..2597)
                     /locus_tag="VII37_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069511.1"
                     /translation="MSTKRIAVIKYAGVTLAAAMILLAQKTSAQQSDGPQTSAPPAVG
                     PVARSSVPRHSGTTERITVPAGTRLAVVLENGISTRDAKAGDSVYLRSSFPVTQNNRI
                     VIPIGSYVRGELLDSKRPGRIKGRGEFRMRLTTLIFPNGYTVDLLGAPRSADSGGNET
                     MDAEGKVTGPGGKGKDAETIAGTTATGAGIGAIASGAKGLGIGAGVGGAAGLAAVLLT
                     RGPDAHLPRGSTLDIVLERDLTLEGDQLRYTDVGQFSPITPPSTREQ"
     gene            2768..2971
                     /locus_tag="VII37_06980"
     CDS             2768..2971
                     /locus_tag="VII37_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069512.1"
                     /translation="MMAKLALIYGMRLLPEDDLGEVRDATVVLKNGRSARVTMHLIEG
                     SKEEIEATFRQSMEAFFEMYPEI"
     gene            complement(3019..3333)
                     /locus_tag="VII37_06985"
     CDS             complement(3019..3333)
                     /locus_tag="VII37_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069513.1"
                     /translation="MDERSFYNEKEEVRKVKLICPSCRGEFEAPVRWKVARKKRELPR
                     GAGEEDKKRFAKASSYMVRLDDLVACYKCRKRFELTGQSTVLINDTLGDPNADPENFG
                     NR"
     gene            complement(3336..>3994)
                     /locus_tag="VII37_06990"
     CDS             complement(3336..>3994)
                     /locus_tag="VII37_06990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5069514.1"
                     /translation="WRTNSSRKLAITFDDGPNPKITPDLLNLLERYEARATFFLVGRF
                     AQECPELVREIAVRGHAIGNHTATHANLFWNSPTQIREQMQRCNEAIATITGIRPKWF
                     RPPWGMRNPWVVSTASAIGMRTALWTILPGDWRAKPASWLIKRMEPIAKRAQSSSISD
                     NNSQPTHGDVLCLHDGDYRFLNADRHATVEALQYWLSRWRDVGLEFVTIDEAVSLPAR
                     "
BASE COUNT          856 a         1115 c         1128 g          895 t
ORIGIN      
        1 acgcgcgtat aggcgttgta ttttgcttgt gcgcgttgca attggtcgcg gggtgcgcgg
       61 aacgcaagaa gccgacagtt tcctgggtcg gggccgggct ttcaaggccc atcgtgcccg
      121 ccactattac ggctcccaag cctcgtccgt tacccgatga aagcgttgac cccgtaccgg
      181 aactacagct ggaaattccc ccaccgcctc cgttgctagc cgttcatact tcacctgcgc
      241 ggcctcatgt tgcgcctgcg ccggtggctg agaacaacgg ggagaacaaa ccggaggctc
      301 cggtcatcgc gccgcaaatg tcagattcgg aggcgtcggc agcccgacaa caaatgacct
      361 ccagcatcaa tgaggcggag aaaaatctcg ctcgttcgca aggcaagaaa ctgaatccca
      421 cgcaatctga cttggcaacc aaaattaaac aatttatagg cgacgcgcag tcggctggtc
      481 gcgaaggaga ttggacgcgc gcgcgcagcc tggctaccaa ggctcaggtt ctgtcagaag
      541 agctggcctc ctcgctataa cagtcagcgt gaccccggaa atcgacgtga atcgccaacc
      601 gcgtccagca gcatcggcat gcgctatgat taatttatga agacgtttga tgtcgctatt
      661 gcgggaggcg gattgatcgg cggcacgatc gcgttcgaat tggcgcgggc tggcctgcat
      721 gtagctgtct tcgaccgcca acagcccggc gaaggctcat cgtgggctgc ggccggaata
      781 ttgtctccgg cccccgaaaa cgcgggaatg atttcaactg ttccgctggg ccgcgcgagc
      841 ttgcagcttt atccagaatt cgtcgccagt gtcgaagaaa gttcgggcca aagcgccggc
      901 tatcgagcgc gaggaacact cgaagcactc ttctccactg atgcgcaaga gaaactcagc
      961 accatcattg ccctccatca tggacttgga ttgaaagcgg agccagtcag cgcggaggat
     1021 gcccgtgaaa tggaggcggc cctgagtcct gatctggagg ccgcggtatt tcggcccgac
     1081 gaagcctgtg tggataatcg ggcgcttaca aaagcggtct taatcgcggc agagcgctcc
     1141 ggcgtaaagc ttttttccgg atctaacgtg caatcgatcg ccaagaacgg agggcgttgc
     1201 gcaggattga ttgtcgacgg caaaaaaata gagtctcgtt ggacagtcat tgccgccggc
     1261 tgcttctctg cagaaatcga aggggctgca caatacgcac ctgtccggcc tgccaaagga
     1321 caaatgattg cgttgcgcgg aaaggatttc agcatcgaac gggtgctgtg gtccgatcac
     1381 gtgtacctcg ttccaaggaa tgagggaaga attcttgccg gcgccacggt tgagtatgtc
     1441 ggcttcgaaa aaaacgtaac tgccggcggc gtgcaaaaaa tcctcaccgc cgcgttggag
     1501 ctcgcaccag ctttggccga tgctcaaata gaggagactt gggcggggtt gcggcctgac
     1561 tcaccggatc atctgccaat cattggtcct acggatctgg acggcctgct aatcgcgact
     1621 ggacattttc gtagcggaat tttgctcgcc cccattaccg ctcaattaat tcgagaatgg
     1681 attacgatgc aaaatgtcac cgtggagtgg gcgcgcttta gtccgacgcg ctttggggaa
     1741 gttgctagag cgacaaattc gtgaagaaat tctccgacac tatatgtaga agcgagttct
     1801 tattgctcgc gcgtcgatgg cggcgtgata ggggagaatt ggcccacgtc tgtataacga
     1861 agctgatcgc cttccagagt caaatcgcgt tccagcacaa tgtccagtgt cgagcctcgc
     1921 ggcaagtgcg cgtccggccc gcgagtcagc aataccgctg ccaatcccgc agcgccgccg
     1981 acacctgcgc caatgcccaa accttttgca cctgaagcaa tcgcgccgat gcccgcccca
     2041 gtagccgtgg tcccagctat cgtttcagcg tctttgcctt ttccgccggg tcccgttacc
     2101 ttcccttccg cgtccatggt ttcatttcca ccgctatccg cgcttcgtgg agcgccgagc
     2161 agatccaccg tgtatccatt tgggaaaatc aacgtggtaa gccgcatgcg gaattctccg
     2221 cgtcccttga ttcgtcctgg gcgctttgaa tccagaagtt cgccgcgaac gtaagagccg
     2281 atgggaatca cgatgcgatt attttgggtg accggaaaag agctgcgcag gtaaacggaa
     2341 tcaccggctt tggcgtctcg tgtggaaatg ccattctcca ggactacggc cagccgtgtt
     2401 cccgctggaa ccgtaattct ctcggtcgtt ccgctatggc gaggaacact cgacctggct
     2461 acaggaccaa cagcaggtgg agccgaagtt tgcgggccgt cagattgctg cgcagatgtt
     2521 ttctgggcca ataggatcat cgcggcagct aatgtcacgc cggcatattt gataacggca
     2581 attcgcttag tggacatgcg cttcataggc accttcccgt aacgtttggt ccgatgctgt
     2641 cgctggaatg gaggatgcct aaagtatcac gttatacatg cccagaccct gcctaattca
     2701 acttgaaggc tgacgctcat tgacctgttt gacagtgcaa gctaggctgg cgtgacactt
     2761 ggaggccatg atggcaaaac tggcgctgat ttacgggatg cgtcttctac ctgaagacga
     2821 tcttggcgag gtgcgcgacg ccacggtagt tttgaaaaat ggacgcagcg ctcgcgttac
     2881 catgcacctg atcgaaggca gcaaggaaga aatcgaggcc acctttcgcc aatccatgga
     2941 agcttttttt gaaatgtatc ctgagatctg atcgacggga gatacgcatc cgcggcgggc
     3001 cgtgctctcc gcaattttct accggtttcc gaaattttct ggatcggcgt taggatcgcc
     3061 gagagtatcg ttgattagca cggtagactg gccagtcagc tcaaaccttt ttcggcattt
     3121 gtaacaggcc accaggtcgt cgagcctcac catataagaa ctggccttcg cgaagcgttt
     3181 cttgtcttct tcgcccgcgc cgcgtggcag ctctcttttc ttgcgggcca ctttccagcg
     3241 caccggggcc tcgaattctc ccctgcaact agggcagatc agttttacct tccgtacttc
     3301 ttctttctcg ttgtaaaaag aacgctcgtc catggttatc gcgcgggaag gctcaccgct
     3361 tcatcgattg taacaaattc aaggcccacg tcacgccagc gcgatagcca gtattgcaaa
     3421 gcttccaccg tggcgtggcg gtcggcattt agaaaacggt agtctccgtc gtgcaggcaa
     3481 aggacgtcac catgggttgg ctgtgaatta ttgtcagaga tgctcgagga ctgcgctcgc
     3541 ttagcgatcg gctccattcg ttttatgagc cacgacgccg gtttggctcg ccaatcgccg
     3601 ggcaggattg tccacaacgc agtgcgcatg ccaatcgcgc tcgcggtgga aaccacccac
     3661 gggttgcgca ttccccacgg cggacgaaac cacttgggcc ttatgccggt aatagttgcg
     3721 atcgcttcgt tacaccgctg catctgctct ctgatttgcg tgggagaatt ccagaagaga
     3781 tttgcatgcg tggcagtgtg gttcccgatg gcatgcccgc gaactgcaat ttcgcggact
     3841 agctctggac attcttgcgc gaatcgtccc accagaaaaa aggtggcgcg cgcctcgtaa
     3901 cgttccagca ggttcaatag gtcaggcgtg attttgggat tggggccatc gtcaaacgta
     3961 atcgcgagtt tccgtgacga gtttgttcgc caca
//
LOCUS       DATCBB010000634         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_118737, whole genome shotgun sequence.
ACCESSION   DATCBB010000634 DATCBB010000000
VERSION     DATCBB010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118737"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1152
                     /gene="argC"
                     /locus_tag="VII37_06995"
     CDS             121..1152
                     /gene="argC"
                     /locus_tag="VII37_06995"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY5069515.1"
                     /translation="MRNLDHIAVVGVTGYTGFELARLLVRHPGVKASTYYMRDTRGAK
                     CLDELFPQLRGRGDAPLQTFSVDAVTSSKAGVAFLATPHDVSAAVAPALVRAGLRVVD
                     LSGAFRFRSAETFTNWYKLAAPPAELLAEAVYGVPEIYEEQIRNARIVANPGCYATSV
                     ILALRPLTDASWIAADTSVVCDCKSGVSGAGKELRRDLQFVEVDENFKAYNLFSHRHT
                     PEILEHTGLNESQDVFSTHLLPISRGILSTIYVTLEASQTGEAVEALYRKFYAGRSMV
                     RIWRAGSLPELQHVAHTNFCDIGFVLDKGGRRMVIVSCLDNLGKGAAGQAVQNFNHML
                     GIEEHTALQ"
     gene            1149..2018
                     /gene="argB"
                     /locus_tag="VII37_07000"
     CDS             1149..2018
                     /gene="argB"
                     /locus_tag="VII37_07000"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5069516.1"
                     /translation="MRIVIKFAGALLEDDQTVRTLASQVAALAKDGHQILVVHGGGRL
                     FTTTLKRMGIESKFVNGLRVTDRESRDVAVMVFAGLLNKKVSSAISAEGQPAVGISAA
                     DAQCFTAEPMMHNEVEGSLGYVGYLTGMNTQFIESLWNDGLVPIAPCLGLGADNELYN
                     INADHMAAACAEFIGADQLIYLTDVAGVLDGERVLSAVGCEEIERLVQCRVVSGGMIL
                     KLEAAKRALEGGVREVRIVGGTGPDALLLASRSKEIKEHQEVTNATGVPGTPGTLVIR
                     EPRSSAQTALSAA"
     gene            2018..>2568
                     /locus_tag="VII37_07005"
     CDS             2018..>2568
                     /locus_tag="VII37_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5069517.1"
                     /translation="MGNASKKSAGSSSARFTQHARLKVAAAKSDSRVEDAEKFLLPTY
                     KRQSFVLERGKGCYVYDAAGKKYLDFLGGIAVNALGHAHPRIVKVIRREAARAIHFSN
                     LYHNAYQGPLARKLAEWSGLDRVFFCNSGTEGIDGAMKLARLCARTAQDAAGQPSKKH
                     RFLALENSFHGRTYGAISVTATE"
BASE COUNT          564 a          658 c          787 g          559 t
ORIGIN      
        1 ggatggatcc aaattcccgg aaattgccgg gaccatcgca ggtgacgaca ccgtgctgat
       61 cattaccccc aataagaaga gtcgcgaatc gctgcaacgg cgcatcgaag ctttgatgaa
      121 atgagaaatc tcgatcacat cgcggtggta ggcgttacag gctacaccgg ctttgagctc
      181 gctcgactac tggtgcgtca tcctggcgta aaagcttcca cgtactatat gcgcgatacc
      241 cggggcgcaa agtgtctgga tgaacttttc cctcaacttc gcggacgagg cgacgctccg
      301 ctgcaaacat tttcggtcga tgccgtaacg tccagtaaag caggcgtagc gtttttagct
      361 actcctcatg acgtgtccgc ggcggtggcc cctgcgttag tgcgagcggg attacgggtg
      421 gtggatttga gtggagcgtt tcgcttccgt tcggctgaaa cgttcacgaa ttggtacaaa
      481 ttggcggctc cgcccgcgga gttgttggcc gaagcggttt atggcgtgcc tgaaatttac
      541 gaagagcaaa taaggaatgc gcgcatcgtg gcgaatcctg gttgctatgc aacgagtgtg
      601 attctggcgc tgcggccact taccgacgcc agttggattg ctgcggatac cagcgtggtg
      661 tgcgattgca aatccggagt tagcggagcc ggcaaggagt tgcggcgcga tttgcaattc
      721 gtggaagttg acgagaattt caaagcctac aatctgtttt cacaccggca cacgcctgaa
      781 attctggaac ataccggcct gaatgaatcc caagacgtct tctccacgca tttgttgccg
      841 atcagccgag gcattctctc gaccatttac gtgacgctgg aagcgagcca aaccggcgag
      901 gcggttgaag ctctttatcg caagttttac gcgggccgtt cgatggtgcg catctggcgc
      961 gccggatcgc tgcccgaatt acaacacgtg gcacacacaa atttctgtga tatcgggttt
     1021 gtgctcgata aaggcgggcg ccgcatggtg atcgtctcct gcctggataa tctgggcaaa
     1081 ggcgctgccg gccaggcggt tcaaaatttc aatcacatgc tggggataga ggagcacacc
     1141 gcactgcaat gagaatcgtg atcaagtttg ctggagcatt attggaagac gaccagacgg
     1201 tgcgcacgct ggctagccag gtcgcggcgc tcgcgaagga tgggcaccag atacttgtgg
     1261 tccatggggg cggccgcctt tttaccacga cgctgaagcg catgggcatt gagagcaaat
     1321 tcgtcaacgg cttgcgagtc acggaccggg agtcacgcga cgtggccgtg atggtttttg
     1381 cggggttgct gaacaaaaag gtttccagtg ctatttcggc ggaaggccag ccagcggtag
     1441 gaatttctgc cgcggacgcg caatgtttta ctgccgagcc gatgatgcac aacgaagtgg
     1501 aaggcagttt gggctacgtc ggctatttga cgggaatgaa tacgcagttt atcgaatcgc
     1561 tctggaacga tgggctggtg ccgatagcgc cctgtctcgg cttgggtgcc gacaacgaac
     1621 tttacaacat caacgctgat cacatggccg cggcttgcgc ggagttcatc ggcgctgacc
     1681 agttgattta tctaaccgac gtcgccggcg tgttggatgg cgagagggtc ctctcggcgg
     1741 tcggttgcga agagatagaa cgactggtgc aatgccgtgt agtttcgggc ggcatgatct
     1801 tgaaactgga agcagctaag cgcgcccttg agggcggcgt tcgtgaagtt cgcattgtgg
     1861 ggggcaccgg tccggatgcg ctgctgttgg catcgcgatc aaaagaaata aaagagcacc
     1921 aagaagtgac caacgcgact ggcgttccgg gaactcccgg cacactggta attcgcgagc
     1981 cccgaagttc cgcgcaaacc gcgctatcgg cggcgtgatg ggcaacgctt ccaaaaaatc
     2041 ggcgggatcc tcgagcgcgc gctttacgca gcatgcaaga ctgaaggttg ccgcggcgaa
     2101 gagcgattcg cgggtagagg acgccgaaaa attcctgctg ccgacctaca agcgccagtc
     2161 gtttgtgttg gagcgtggca agggttgtta tgtgtatgac gcggccggaa aaaaatatct
     2221 cgattttctc ggcgggattg cggtcaatgc tttgggccac gcgcatccac gaattgttaa
     2281 ggtgattcgt cgcgaagccg cgcgcgccat tcatttttca aatctctatc acaacgcgta
     2341 tcaaggaccg ctggctcgta agcttgcgga atggtccggc ctcgatcgcg tgttcttctg
     2401 caatagcggt acagaaggaa tcgatggcgc gatgaagctt gcgcgcttat gtgcgcgaac
     2461 ggcccaggac gcagcaggac aaccctcaaa aaagcaccgc tttctggcgc tggagaattc
     2521 ttttcacggg cgcacctatg gcgcaatcag cgtcaccgcg actgaaaa
//
LOCUS       DATCBB010000635         3699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_182522, whole genome shotgun sequence.
ACCESSION   DATCBB010000635 DATCBB010000000
VERSION     DATCBB010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182522"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..894
                     /locus_tag="VII37_07010"
     CDS             187..894
                     /locus_tag="VII37_07010"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5069518.1"
                     /translation="MNPFDSSIITFLNSFARHSWAFDSFVYMLSGNDLLKGGVVVALI
                     WWIWFRPNADKDDTRQHLICAIFACLLAVLIARALAILLPFRARPMAVTALHFQHPYG
                     AGDGGLIDWSSFPSDHAAVFFALAMSIFFVWRAAGSAALSYVFLFIAMPRIYLGFHYP
                     SDVLGGAFIGIALAFLARAKLFCDWVRRFIMPWLQRSPSSFYACLFILTFQIATLFQS
                     MRFIARFLFSLRHSHNI"
     gene            1026..3323
                     /locus_tag="VII37_07015"
     CDS             1026..3323
                     /locus_tag="VII37_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEY5069519.1"
                     /translation="MNRWAVVGSVLFSVLALTLAGAYKVVKVSADDQSLAGYSTESSR
                     AERQWEEKLREIPSPENLRAYMQHLSARPHNVGTAYDKENAEWIAAKFKDFGLDTHIE
                     QFDVLYPTPKERVVELVEGGPKFSAKLQEPALPEDPTSNQQAEQLPTYHAYSKDGDVT
                     APLVYVNYGIPEDYEQLDRMGVSVKGKIVIARYYHSWRGIKPKVAAEHGAVGCLIYSD
                     PHEDGFVQGETFPKGAWRPKDGVQRGSVADMPVYPGDPLTPGVGATKDAKRLKIEDAQ
                     TITKIPVLPISYADAQPLLEALTGRVAPPQWRGGLGITYHVGPGDAKVHLKVKSNWLT
                     KPVYDVIAKIPGTTLPDEWVLRGNHHDAWVNGAEDPTSGMVAVLEEARALGELLKSGW
                     KPKRTIIYCAWDGEEPGLLGSTEWAETHYDELRTHAVAYINSDSNGRGYLEIEGSHTL
                     EKFANDIARDIADPETKLSAWKRNHLHEIAEAKSPEERDKMRQRPDERIGALGSGSDY
                     TAFLQHDGVASMNIGFGGEDGGGIYHSIYDDFYWYTHFSDTNFQYGRALAQTGGTAIL
                     RLADADLLPFEFGDFADTMDTYVKELKTLAQKTQADIQERNREIEEGVFQATDDPKKP
                     LIVPSVEPAAPYLNFAPLDNATVALGRSATEYRKALEKINANGGNAMAAASLADVNKL
                     LIQSERKLTNTEGLPNRPWFKHQIYAPGFYTGYAAKTMPAVREAIEQKQWKQAEAGIV
                     VVASVLQDEAELISAAAAKLGTAAH"
     gene            3440..>3699
                     /locus_tag="VII37_07020"
     CDS             3440..>3699
                     /locus_tag="VII37_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069520.1"
                     /translation="MRVLIAKESELRRRYDLRWRLTGVATRRSGWLADPHGLNPIALL
                     NGHWPPQRETPPKDIREWLEKSHADVFVEASSLNLQSGQPAI"
BASE COUNT          841 a         1074 c          949 g          835 t
ORIGIN      
        1 gccagttagc tgaggtgctc attttaccgg cgataccgga cctacagtaa cgtcccgcat
       61 ttgctagtga gtgatacaac atggtcatgg gcaatcatgg acaagcgata atttttcaga
      121 tggtatgctg cgattcggta gtaaataggt tcctccccca attgctgtta agcacttaaa
      181 atattcatga accccttcga ttcctccatc attacttttc tgaattcgtt tgcgcgccat
      241 tcatgggcct tcgactcctt tgtttacatg cttagcggca acgatttgct caaaggtgga
      301 gtagttgtgg cgctgatctg gtggatctgg ttccgtccca acgccgacaa ggacgacact
      361 cgtcaacatt tgatatgcgc gatttttgcg tgtttgctcg cggtgctcat tgcgcgcgcc
      421 ttggcgatcc tgcttccttt tcgtgctcga cccatggccg tcacagcact ccactttcag
      481 catccgtatg gagccggcga cggcggactc attgactgga gctcttttcc gagcgaccac
      541 gcggcggtgt tttttgcctt ggcgatgtcc atcttcttcg tatggcgcgc agcaggaagt
      601 gcggctttgt cttatgtctt tttattcata gccatgcctc gcatctatct tggattccac
      661 tatcccagtg atgttctggg cggagcattc atcggaatcg cgctagcgtt tttagcgcgc
      721 gccaaactgt tttgtgattg ggtacggcgc tttatcatgc cgtggttgca acgttcaccg
      781 agcagctttt acgcctgttt atttattctg acatttcaaa ttgccacact gtttcagtcc
      841 atgagattca tcgctcgctt tttattttcc ctccggcact cgcataacat ttagccatcg
      901 agaaaacctg ctcgtgcagc ggtgcgttct ggacgtattg gggtcgtcat ttctgctgat
      961 gaatccttcc gcattccaaa ctaagccatc ttcaggcaga atgtctccgc cattcaggag
     1021 acgttatgaa tcgctgggct gtggttggat ccgtcttatt cagcgtgtta gcgctgacgc
     1081 tcgcaggtgc ctataaggtt gtaaaagttt cggccgacga tcaatcgctg gcgggttact
     1141 caacggaatc atcgcgcgcc gaacgccagt gggaagaaaa gcttcgcgaa atccccagcc
     1201 cggaaaatct gcgcgcctac atgcagcatc tgtcggcgcg tccgcacaat gtcggcaccg
     1261 cttacgacaa ggaaaacgcc gaatggatcg ccgcgaaatt caaagacttc ggtctcgata
     1321 cccacattga acagttcgac gtgctttatc ctacgcccaa ggaacgcgtc gtggagctcg
     1381 tggaaggcgg gccaaaattt tcagcgaaac tccaggaacc cgcgctaccg gaagacccaa
     1441 cttcaaacca gcaagccgaa cagctcccca cctaccacgc ctactccaag gatggcgacg
     1501 ttaccgcccc gctcgtttac gtgaactacg gaatccccga agattacgaa caactcgatc
     1561 gcatgggtgt ttccgtgaag ggcaagatcg tgatcgcccg ttactaccat tcttggcgag
     1621 gcatcaagcc aaaagttgcc gcagaacatg gcgctgtcgg ctgcctgatt tattccgacc
     1681 ctcatgaaga cggatttgtg caaggtgaaa ccttcccgaa aggcgcgtgg cgccctaagg
     1741 atggcgtgca gcgcggcagc gtggcagaca tgcccgttta tcccggtgat ccgttgacgc
     1801 cgggcgtggg cgccaccaaa gatgccaagc gcttgaagat cgaagatgcg caaactatta
     1861 caaaaattcc agtcttgccg atttcctatg ccgacgccca gccgctcctc gaagcgctaa
     1921 ccggacgtgt cgcgccaccg caatggcgcg gcggcttggg catcacctat cacgtcggcc
     1981 ccggcgacgc aaaagtgcat ctcaaggtga aatcaaactg gctcaccaag cctgtttacg
     2041 atgtcatcgc aaaaattccc ggcacgactc ttcccgacga gtgggtgtta cgcggaaatc
     2101 atcacgacgc ctgggtgaat ggcgcggaag atccaacttc tgggatggtc gcagtgctgg
     2161 aagaagcccg cgccctcggt gaattactga agtccggatg gaagcccaag cgcaccatca
     2221 tttattgcgc gtgggatggc gaagagccgg ggttactcgg atccacggaa tgggctgaaa
     2281 ctcattacga cgaattgcgc acgcatgctg tcgcgtacat caactcggat tccaacggca
     2341 gaggctatct ggaaatcgaa ggttcgcata cgctcgaaaa attcgccaac gacatagccc
     2401 gcgacatcgc cgatccggaa accaaactct ccgcctggaa gcgcaaccac ctccacgaaa
     2461 ttgccgaagc caaatcaccc gaagagcgtg acaaaatgcg ccagcgcccg gacgaacgca
     2521 tcggtgcgct cggctccggc tctgactaca ccgcttttct gcaacacgat ggcgttgcct
     2581 ccatgaatat cggcttcggt ggagaagacg gcggcggcat ttaccattcc atttacgacg
     2641 acttctactg gtacacacat ttcagcgaca ccaattttca gtacggtcgc gccttggcgc
     2701 aaaccggtgg caccgcgatt ttgcggctgg ctgatgccga tctattgccc ttcgaattcg
     2761 gcgatttcgc cgacaccatg gatacctacg tgaaagaatt gaaaacgctg gctcaaaaaa
     2821 cgcaggccga tattcaggaa cgcaaccgcg agatcgaaga gggtgtattc caggccaccg
     2881 acgatccgaa aaaaccgctg atcgtgccgt ccgtcgaacc tgcagctccg tatttaaact
     2941 tcgccccgct cgacaacgcc acggtggcac tcgggcgcag cgctacggaa tatcgcaagg
     3001 ccctcgagaa aataaatgca aatggcggca acgccatggc cgctgcctct ctcgccgatg
     3061 tgaacaaact tttgatccag agcgagcgaa aacttaccaa caccgaaggc ttgccgaacc
     3121 gcccgtggtt taaacatcag atttacgcgc ccggttttta caccggctat gccgccaaaa
     3181 ccatgcccgc ggtgcgcgag gctatcgaac aaaaacaatg gaaacaagcc gaggccggca
     3241 tcgtggtcgt ggccagcgtt ctgcaagacg aagccgagtt gatttccgca gcggccgcaa
     3301 aacttggcac cgccgcacac tagctggcta tttcagtttt ttgcaagtag cgaaaattac
     3361 cgcggacgct tggaaggact gacccattcc ttcatacaat ctcgctctaa tcggtttcgg
     3421 caatgtcggc cgcgcttttt tgcgcgtgct catagccaaa gaatccgagc tccgtaggcg
     3481 ctatgatctg cgctggcgtt taactggcgt tgccacccgc cgctccggct ggctcgctga
     3541 tcctcacggc ctgaatccca tcgcattatt aaatggccat tggccgcccc agcgagaaac
     3601 accgcctaaa gacattcgag aatggctcga gaaatcgcac gccgatgtct tcgttgaagc
     3661 gagttcgctg aacctccagt ccggccagcc cgccatcga
//
LOCUS       DATCBB010000636         1346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_345019, whole genome shotgun sequence.
ACCESSION   DATCBB010000636 DATCBB010000000
VERSION     DATCBB010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345019"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VII37_07025"
     CDS             complement(<1..343)
                     /locus_tag="VII37_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069521.1"
                     /translation="MRWPILSGSPVLDSLRPVIENSREVRTNIGKIVEVAGWMGYEEL
                     PLPEFTLPFGVGANDANEAIDFILVADSIDTAFTDFSTHEKFQVDFAGQHWSDSEAEF
                     ACLKRALDAGKP"
     gene            complement(530..>1346)
                     /locus_tag="VII37_07030"
     CDS             complement(530..>1346)
                     /locus_tag="VII37_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY5069522.1"
                     /translation="SLANRCDIPVAGGARHPLFQKLITAEFWHGKNGLANIELPRSKC
                     KADPRFGPDLIIELIHASPHEITLVPVGPLTNIALAVERDPSIVPLIKEVILMGGSIK
                     GGNVNAAAEANIYNDPEAAQIVFQAGWPLTMVGLDVGDKTLLGHKQLDQLASTHGPVN
                     DFIYGVTKFLVELSEKFGDSGTPMYDPLAVGVAIDSTLVTAPAMHVDVETRGEFTRGE
                     TVANRRNEIERNVLHGDRYIIEGVDRVEPNAKVCVEVQAERFLQLFVSRIQGK"
BASE COUNT          321 a          409 c          298 g          318 t
ORIGIN      
        1 tcggttttcc cgcgtccaac gctcgcttga ggcaagcgaa ctccgcctcc gagtccgacc
       61 aatgctgccc ggcaaaatca acttgaaatt tctcgtgcgt ggagaaatcg gtgaacgccg
      121 tatcgatcga atccgctacc aaaatgaaat cgatcgcttc attcgcatcg tttgcgccga
      181 ccccaaaggg aagcgtgaat tccggaagcg gcaattcctc atatcccatc catccggcaa
      241 cttccacaat tttcccgata ttggtgcgca cctcacgaga attttctatg actggtcgta
      301 agctatccag aaccggacta ccgctcaaaa tcggccagcg cacagggttt tccttcggga
      361 cactcgttga atttttcatt aagcaggagg caaaaactcg attcaccggc aaaatcgcca
      421 gcgatccagc caaatccgta aggaagcgcc gtctttccct gatcgctgat cgcatttata
      481 actgaagtcg caaaaaaaat aaacgaacgt tctcgcgcca gccctcggct cacttgccct
      541 gaatgcgcga aacaaaaagt tgcaaaaaac gttccgcctg cacttcgaca cacactttgg
      601 cgttgggctc cactctatca acgccttcga tgatgtatcg gtccccatgc agaacgttgc
      661 gctcaatttc gttccgccgg ttggcgacgg tttctccgcg cgtgaattcg ccccgagtct
      721 ccacatcaac gtgcatcgcg ggcgccgtca ccaacgttga gtcgatcgcc actccaaccg
      781 ccagcggatc gtacatcggc gtaccgctat cgccgaattt ttcagaaagt tccaccagaa
      841 acttggttac tccataaatg aaatcattaa ccggtccgtg cgtgctggct aattgatcca
      901 attgcttatg ccccagcagc gttttgtcgc ccacatccag gcccaccatg gtcagtggcc
      961 agccagcctg gaaaacaatt tgtgccgctt ccggatcgtt ataaatgttt gcttccgccg
     1021 ccgcatttac atttcctcct ttgattgacc cgcccatcag gattacttcc ttaatcagcg
     1081 gcacaatgga gggatctctt tccactgcca acgctatatt cgttagcggc cccactggca
     1141 cgagcgtgat ctcgtgcggc gacgcgtgaa ttaattcaat gatcagatca ggcccaaaac
     1201 gcgggtccgc tttgcatttg ctgcgaggca attcaatatt ggccagccca ttttttccgt
     1261 gccaaaattc cgccgtaatc aatttttgaa acagcggatg ccgcgcgcct cccgcaaccg
     1321 gaatgtcaca acgattagcc agcgaa
//
LOCUS       DATCBB010000637         1744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_53564, whole genome shotgun sequence.
ACCESSION   DATCBB010000637 DATCBB010000000
VERSION     DATCBB010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53564"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="argS"
                     /locus_tag="VII37_07035"
     CDS             complement(<1..1269)
                     /gene="argS"
                     /locus_tag="VII37_07035"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY5069523.1"
                     /translation="MYLKLTTRFREALGAHIREKYGLDIGIVTERPPKIEMGEAASPV
                     SFELAKRLKRAPRQIAQEIAGSLAPIEGIANVEVAGAGYLNAYFERTNFLRKVQREAG
                     EAAVPDRAGKIIVEHTSINPNKAAHIGHVRNAVLGDTIARVLRHTGGAVQVQNYIDNT
                     GVQVADVVIGFVYMEKKNLAQVRELAAQSKFDYLCWDVYARASQFLAEDKTRAAALRG
                     ETLKAIEAGRGEIAEMGALIADAIVSLHLQTMARLGIFYELLARESEILHLHFWDAAF
                     EQLKAAGAIRLATSGKMAGCWIMPAKEKAGAEKEKVAATESPDTNDSADADSDEDLNN
                     QDKIIVRSNGTVTYVGKDIAYQLWKFGLLGKDFFYSKWPDAPGSHTLWSTASTVGDPS
                     APNFGAPAKTVYNVIDSRQAYLQDVVSEGLR"
     gene            complement(1360..>1744)
                     /locus_tag="VII37_07040"
     CDS             complement(1360..>1744)
                     /locus_tag="VII37_07040"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEY5069524.1"
                     /translation="ALAYWLLGAVWYGVLFHNAWMELEHVTPEQASAAGTPIAPYIIS
                     FVLNLVIGFVLAQLCAWRNANTAARGAALGILIWIGFLGPVTYTTYMYEMRPKQLFAI
                     NEFYVLVGMCLMGAILGAWPKKQIA"
BASE COUNT          398 a          546 c          395 g          405 t
ORIGIN      
        1 ccgcaagcct tccgagacca catcctgcag atacgcctgc cgggaatcga ttacgttgta
       61 gacagtcttc gctggagccc caaaatttgg cgctgacgga tcaccaaccg tgctcgcggt
      121 actccataaa gtatgtgacc ccggcgcatc gggccactta ctgtagaaaa agtccttacc
      181 aagcaaacca aatttccata attggtaagc gatatctttc ccgacgtagg tcaccgtgcc
      241 attcgaacgc acaataatct tgtcctgatt attgagatct tcatcagagt ccgcgtctgc
      301 gctgtcgttg gtgtcggggc tctccgtcgc cgccaccttc tctttttccg cgccagcttt
      361 ttcttttgcg ggcatgatcc agcaaccggc catcttcccg ctagtcgcta gccgaatcgc
      421 cccggccgcc tttaactgtt caaacgccgc atcccaaaaa tgtaaatgta aaatttcgct
      481 ctcgcgcgcc agcagttcgt agaaaattcc caggcgcgcc atggtctgca gatggagact
      541 gacgatggca tccgcaatta gtgcgcccat ctcggcaatt tcaccgcgtc ctgcttcgat
      601 ggccttcaaa gtctcgccgc ggagtgccgc tgcgcgagtt ttgtcttcgg ccaggaactg
      661 cgaggcccga gcgtacacat cccaacacag gtaatcgaat ttggactgcg ccgccagttc
      721 gcgtacctgt gcaaggttct tcttctccat atacacaaat ccaataacca catcagcgac
      781 ctgcaccccg gtgttatcga tgtaattttg cacttgcacc gcgccgccag tgtgccgcag
      841 cactcgcgcg atggtgtcgc ccagcaccgc gtttcgcaca tgcccgatgt gcgccgcctt
      901 atttggatta atgctggtgt gctcaactat aatttttccg gcacgatccg gtacggccgc
      961 ctcacctgcc tcgcgttgca cctttcgcaa aaagttcgtg cgctcaaaat aagcatttag
     1021 atatccagcc ccggcaactt caacgtttgc aatcccttcg atgggcgcga ggctgcccgc
     1081 aatttcttgc gcgatctgtc gcggcgcacg cttcagccgt ttcgccaatt caaaagaaac
     1141 gggtgaagcc gcctcgccca tttcgatctt cggcgggcgt tccgtaacaa tcccaatatc
     1201 aagcccatat ttctcgcgga tgtgcgcgcc taacgcctcg cgaaatcgcg ttgtcagctt
     1261 cagatacacg tttcctcaat cccctgaatg aacttcacca tctagaatga acatcaagga
     1321 gtgaaatgga aagtagttgt tcggcaatct caacgaaact tatgcaattt gtttcttcgg
     1381 ccacgcgccc agaatggcgc ccatcaaaca cattcccacc agcacataga attcattaat
     1441 cgcaaacaac tgcttgggtc gcatttcata catataagtc gtgtacgtga ccggtcccag
     1501 aaatccgatc catatcagta tacccagcgc cgctccccgt gcagccgtgt tggcatttcg
     1561 ccacgcacaa agttgcgcca gcacaaaccc gataactaag ttcagcacaa acgaaatgat
     1621 gtacggcgcg ataggggtcc ctgcggcact ggcctgctcg ggcgtcacat gctcaagctc
     1681 catccaggcg ttatgaaaca gcactccata ccacaccgct cccagcagcc aataagctaa
     1741 cgcg
//
LOCUS       DATCBB010000638         3828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_182639, whole genome shotgun sequence.
ACCESSION   DATCBB010000638 DATCBB010000000
VERSION     DATCBB010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182639"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VII37_07045"
     CDS             <1..1492
                     /locus_tag="VII37_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069525.1"
                     /translation="LGELERYGIPTTAKLSRATMLHVAEDLDADFVVFGSFNYDGKNL
                     TIDSRLLRVNPASLLPPLHESGPLETVMDLHTRLMWRMLTENDKAYPLSLTEFGQMHK
                     PIRLDAFEHYSRGLIANEDETRLRELREAVRLEPEWVEANYALGEAYFTRRDCASALP
                     WYAKVPKTHDRYLEALFSIGVCKLSLNQPDQAEEAFTTLQSLLHGSSAPGVDFPEVLN
                     NLAIAKARQNKLPAAQEDLKRASALDPDEDDYPFNLGLIAIRQGDFNGAAALFREASH
                     REPDIPEDRTLLVAALEKAGKQEEADQERNAALEAFGPNGVTTTHLDAKNDSLTHFER
                     VTTEIDPETLRFEMESVEARSATSGAQDARETPEDTPASHLRRARQALSGGQLDAAER
                     EYHVTLASDKGNASAHLGLGEIARRQGRLDDSVKELLLSLEARDSAVVRTTLARVYLE
                     QKKPALARMEVERALKLAPNYAEAKQLLEHLQSGKAAEVSPKGGPQ"
     gene            1489..2886
                     /locus_tag="VII37_07050"
     CDS             1489..2886
                     /locus_tag="VII37_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY5069526.1"
                     /translation="MKRSTGFLTRWPERLGAAGVVATVLCVVCLALVLGVRLNAAAAV
                     EQQTSTEVVELELNGEVEPILATYMEEGLASAAQRHAALVLITMDTPGGLSDSMKNII
                     QHILASPVPVAVYVEPTGARGASAGFYILLSADIAAMAPGTHAGAASPVIIIGGFAQQ
                     IDETFRKKLNNDAMAFLRSFTERRGRNPALAETAITEAKAFTEKECLDQKMIDVVASS
                     REDLLRQLNGRSITRFDGSKVTLALEHPTFVPFELSARQKFLARIVEPDAFFILLLIG
                     VLGLYTEFTHPGVVAPGVFGGICAVLALYAMHLLPVNYAGLFLILLALGFFILEAKFT
                     SHGVLALGGIVSMLLGAMFLVRSPLTNGGVSLGIALAATLPFAALTVFLMRLVLRSRK
                     WKAATGMEELIGEQGIAVSALRAGVEGMIRIHGELWGAVSSQEAPEGAPVRVLRIEGL
                     KLYVEPARSAAVSHS"
     gene            2927..3718
                     /locus_tag="VII37_07055"
     CDS             2927..3718
                     /locus_tag="VII37_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY5069527.1"
                     /translation="MEIGFLGIVVIIGIGLIWFWNSLYIIKEWERGVVLRLGRLLPSA
                     KTAGINLVFWPIETLYRINMRLETLDVPPQDIITRDNVSVKVNAVCYFRVVDANLAMS
                     QVQNYQYATSQLAQTTLRSIVGQFELDEILSQRDKVNAKLQIILDTDTEPWGIKVSKV
                     EVKQVDIPEQMQRAIARQAEAERERRAKIIHAEGEYEASQKLADAAKVLETQPTAIQL
                     RYLQTLTEIGTEKNTTIVFPLPIDIINQWTRALGSGPGSGQISAK"
BASE COUNT          813 a          849 c         1319 g          847 t
ORIGIN      
        1 gttgggggag ttggagcgct acggcattcc caccacggcg aaattgagcc gcgcgacgat
       61 gttgcatgtc gcggaagatc tggacgccga tttcgtggtg tttgggagtt ttaattacga
      121 cggaaagaat ttgacgattg atagtcggtt gttgcgggtg aatccggctt cactgttgcc
      181 gccgctgcac gaaagcgggc cgctggaaac agtgatggac ctgcacacgc ggttgatgtg
      241 gcggatgctg acggagaacg ataaggcgta cccgttgagc ctgacagaat tcggccagat
      301 gcataagccg atccgtctgg atgcgtttga gcattacagt cgcggattga ttgctaacga
      361 agacgaaacg cgcctgcggg agttgcggga agcggtgcga ctggaaccgg aatgggtaga
      421 agcgaactac gctctcgggg aagcttattt tacgcgaaga gattgcgcgt cggcactgcc
      481 gtggtatgcc aaagtgccga agacgcacga tcgttattta gaggcgctgt tttcgatcgg
      541 ggtttgcaag ttgtcgctga atcagccgga ccaggcggaa gaagcgttca cgacgttgca
      601 aagtttgttg cacggcagca gcgcgcctgg agtggatttt ccggaggtgt tgaataatct
      661 tgcgattgcg aaggcgcggc aaaataaact tcccgcggcg caggaggatt tgaagcgcgc
      721 gtctgcgctg gatccggatg aagatgatta tccgtttaat cttggactga tcgcgatacg
      781 ccagggagat tttaacgggg ccgcagcgct atttcgcgaa gcgtcgcatc gcgagccgga
      841 tattccggag gaccgcacgt tgctggtggc ggcgcttgag aaagcgggta agcaggaaga
      901 ggcggaccag gagcgcaatg cggctctgga ggcttttggt cccaatggcg tgacgactac
      961 gcatttggat gcgaagaatg attcgttgac gcatttcgaa cgggtgacca cggagattga
     1021 tccggagacg ctacgctttg agatggagtc ggtggaagcg cggtccgcaa caagtggggc
     1081 gcaagacgcg cgcgagacgc ctgaagatac gcctgcatcg catctgcggc gcgcgaggca
     1141 ggcactttcc ggcgggcaac tggacgccgc ggagcgcgaa tatcacgtga ccctggcctc
     1201 cgacaaaggg aatgcgtccg cgcatttggg gttgggcgaa attgcgcggc gtcagggacg
     1261 gttggacgat tcggttaagg agttactgct gtcgctggag gcgcgggatt cggcggtagt
     1321 gcgcaccacg ttggcgcgcg tttacctgga gcagaaaaaa cctgcgttgg cgcgcatgga
     1381 agtggagcga gcgctgaagc ttgcacccaa ttatgcggaa gccaagcaat tgctggaaca
     1441 tttacaaagt ggaaaggcgg cggaggtgtc tccgaaagga gggccgcaat gaaacgctcg
     1501 actggatttt tgacgcgctg gcctgaaagg cttggggccg ccggagtggt cgcgactgtt
     1561 ttgtgcgtgg tgtgtttggc gttggtgttg ggcgtgcgct tgaacgccgc ggcggcggtg
     1621 gaacagcaga cctcgacgga agtggtggag ctggagctca acggggaagt ggaaccgatt
     1681 ctggcgacgt acatggaaga ggggctggcg agtgcggcgc aacggcatgc cgcgttggtg
     1741 ctgatcacca tggatacgcc cggtgggctc agcgactcca tgaagaatat tatccagcac
     1801 attttggctt cgccggtgcc ggtggcggtt tatgttgagc cgactggggc gcgcggagct
     1861 tcggcgggat tttatatttt actctcggcg gatattgcgg ccatggctcc gggcacgcac
     1921 gcgggcgcgg catcgccggt cattataatt ggcggattcg cgcagcagat tgacgaaaca
     1981 tttcgcaaga aacttaataa tgatgcgatg gcgtttttgc gcagcttcac ggagcggcgc
     2041 gggcggaacc ccgcgctggc agagacggcc atcacggaag ccaaggcgtt cacggaaaaa
     2101 gaatgcctgg accaaaagat gatcgatgta gtggccagtt cgcgggagga tttgctgcga
     2161 cagttgaatg gaagatcgat cacgcggttt gacggaagca aggttacgct ggcgttggag
     2221 catcccactt ttgtgccgtt cgaattgtcg gcgcggcaga aatttttggc acgtattgtg
     2281 gagcccgatg cgttttttat tctgttgctc attggggtgc tggggcttta tacggaattt
     2341 acgcatcctg gagttgtggc gccgggagtt tttggcggga tttgcgcggt gctggccctt
     2401 tacgccatgc atttgctccc tgtgaattat gcgggcttgt ttttgatttt actggcgttg
     2461 ggatttttca ttctggaagc gaagttcacg agtcacgggg tgctggcgct gggcggaatt
     2521 gtgtcgatgc tattgggagc catgttcctg gtgcgctcgc cattgacgaa tggcggagtg
     2581 agcttgggaa ttgcgctggc ggccacgctg ccgttcgccg cgctcacggt gttcttgatg
     2641 cggctggtgt tacgctcgcg aaaatggaaa gcagcaacgg ggatggaaga attgatcggg
     2701 gagcagggca tcgcggtgtc ggcgttgcgt gcgggtgtgg aaggcatgat tcgaattcat
     2761 ggtgaattgt ggggcgcggt gtcttcgcag gaggcgccgg aaggtgcgcc ggtgcgggtg
     2821 ttgcgaatcg aagggttgaa actttatgtg gaaccggcgc ggtcggcggc ggtgagtcac
     2881 agctaagaga caaagttaag aggttgtccg gcttggggag gacagcatgg agatagggtt
     2941 cctgggaatc gtggtgatta tcggcattgg gctgatctgg ttctggaatt cgttgtacat
     3001 cattaaggaa tgggagcgcg gagttgtgtt gcggctgggg cgcttgctgc cgtcagcgaa
     3061 aacggcggga atcaatctgg ttttctggcc gatcgaaacg ctttaccgca tcaatatgcg
     3121 gctcgagact ttggacgtgc cgccgcagga catcattact cgcgataacg tttcggtgaa
     3181 ggtgaacgcg gtttgttatt tccgggtggt ggatgcgaac ctggcgatgt cgcaggtgca
     3241 gaattaccag tacgcgactt cgcaactggc acagaccact ttgcgtagca tcgtcgggca
     3301 attcgaattg gacgaaattt tatcgcaacg cgataaagtg aatgccaaat tgcagattat
     3361 tctggacacg gacacggagc cgtggggcat caaagtttcc aaggtggaag tgaagcaggt
     3421 ggatattccc gagcagatgc agcgggccat tgcgcgtcaa gcggaagcgg aacgcgagcg
     3481 gcgggcaaaa attattcatg ccgaaggcga gtacgaagcg tcgcaaaaac ttgcggatgc
     3541 cgccaaagtt ctggagacac aaccaaccgc gattcaactg cgttatctgc aaactttgac
     3601 ggaaataggt actgagaaga acaccacgat tgtgtttcct ttgcccatcg atattattaa
     3661 ccagtggacg cgggctttag gaagtgggcc gggtagcgga caaatcagcg cgaaataagt
     3721 agaagataag gttattgatg gcggcgaaaa aaagaggtgc gggcgagcga gtgctggaaa
     3781 gccggcacgt catcggctta tttctgctga tgctggtttt ttccgcgg
//
LOCUS       DATCBB010000639         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_247356, whole genome shotgun sequence.
ACCESSION   DATCBB010000639 DATCBB010000000
VERSION     DATCBB010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247356"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="rplF"
                     /locus_tag="VII37_07060"
     CDS             complement(<1..297)
                     /gene="rplF"
                     /locus_tag="VII37_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY5069528.1"
                     /translation="MSRIGRKPIPLPSGVKVAMQGGKIDVQGPKGKLSFAIPRGISFE
                     QKDGVLTALRETEDHRALHGLARALVANAVHGVTQGFKKDLDIVGVGYRAELKGK"
     gene            complement(368..766)
                     /gene="rpsH"
                     /locus_tag="VII37_07065"
     CDS             complement(368..766)
                     /gene="rpsH"
                     /locus_tag="VII37_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY5069529.1"
                     /translation="MQTDPIADFLTCIRNAAAAKHQRVDVPASRLKSEIARILKDEGY
                     ISTYKMVDENKTRKMLRVFLKYTPDRRSVITGLKRISRPGARRYLGVTDIRPVVGGLG
                     ISIMTTPKGLMSGRAARKAKMGGEILCEVW"
     gene            complement(782..967)
                     /locus_tag="VII37_07070"
     CDS             complement(782..967)
                     /locus_tag="VII37_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY5069530.1"
                     /translation="MARLAKIAKSLKKPKFKVRVRNRCRVCGRARAYIRKFQMCRICF
                     RGMALKGEIPGVTKASW"
     gene            complement(968..1525)
                     /gene="rplE"
                     /locus_tag="VII37_07075"
     CDS             complement(968..1525)
                     /gene="rplE"
                     /locus_tag="VII37_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY5069531.1"
                     /translation="MKSRLQEKYRNETVPALMKRFGWKNNMAVPKLQKITVNIGLGEA
                     SQNAKLLDTAAQELGQITGQKAMITKAKKSIANFKIRKGMPIGCAVTLRGDKMYEFLD
                     RLCNVVLPRVRDFRGLPFNAFDGRGNYTLGLKDQLVFPEIDYTRVDKIKGMNVTLTTT
                     AKNDEEGRELLKGLGFPLRVPGGAQ"
     gene            complement(1561..1878)
                     /gene="rplX"
                     /locus_tag="VII37_07080"
     CDS             complement(1561..1878)
                     /gene="rplX"
                     /locus_tag="VII37_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY5069532.1"
                     /translation="MAIRKGDNVRVIAGKDAGKSGRVLSIDAKKNRVVVEHVAIMKRH
                     TRPNPQKNIKGGIVEKEASINVSNVMVVCASCGKHTRIGHTVLPDGSKVRSCRRCNTA
                     LDK"
     gene            complement(1918..2289)
                     /gene="rplN"
                     /locus_tag="VII37_07085"
     CDS             complement(1918..2289)
                     /gene="rplN"
                     /locus_tag="VII37_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY5069533.1"
                     /translation="MSIMMRTWLTVADNSGARKLMCILPLGGDAGLKAGLGDVITASV
                     KEATPESAVKQGTVVKAVVVRMRKEARRKDGTYIRFDDNAAVLINDAGEPVGTRVFGP
                     VARELRERQYTKIVSLAPEVW"
BASE COUNT          458 a          710 c          575 g          564 t
ORIGIN      
        1 tttgcccttg agctccgcgc gatagcctac gcccacgatg tcgagatctt ttttgaagcc
       61 ttgcgtaacg ccgtgcaccg cattcgctac gagagcgcga gcaagaccgt gtaatgcgcg
      121 atggtcttcc gtttcgcgca aagccgtgag tacgccgtct ttttgctcaa aagaaatacc
      181 gcgcgggatc gcgaaagaca acttgccctt gggtccttgc acatcgatct tgccgccctg
      241 catggcgact ttaacgccag aaggaagcgg aattggttta cgcccgatac gtgacatcag
      301 ttctatccct taactacgaa cgcgcttcga caaaaattgc tgatgcagac tttgttacca
      361 gacttcgtta ccagacttcg caaagaatct cgccgcccat tttggcttta cgcgcggcgc
      421 ggccgctcat caagcccttg ggcgtggtca tgatactgat gcccagcccg cccaccaccg
      481 gacgaatgtc cgtaacgccc agataacggc gcgctcccgg acgcgaaatg cgcttcagcc
      541 cggtgatcac actgcggcgg tccggggtgt acttcaggaa aacgcgcagc atcttgcgag
      601 tcttgttttc atccaccatt ttgtaagtgg aaatgtagcc ttcatctttc aagatgcgcg
      661 caatctcgga cttgagccgc gacgcgggca catccactct ttggtgcttg gcggccgccg
      721 cgttgcggat gcacgtcaga aaatcggcaa tgggatccgt ctgcatattt ctcctaaata
      781 cctaccagct agctttcgtg acgccgggaa tttcaccctt gagcgccata ccgcggaaac
      841 aaattctgca catctgaaac ttgcgaatgt acgcgcgcgc gcggccgcaa actcggcaac
      901 ggttgcgcac gcgaacctta aacttcggct tcttcaaact tttggcgatt tttgcaaggc
      961 gtgccattta ttgcgctcct cctggaacgc gaagcgggaa gccaagcccc ttcaggagct
     1021 cgcgaccttc ttcatcgttt ttcgcggtgg tggtcaaggt cacgttcatg cccttgattt
     1081 tgtccacgcg ggtgtaatca atttcgggaa acaccaactg atctttcaag ccgagcgtgt
     1141 aattgccgcg gccatcgaat gcgttaaacg gcagaccgcg aaaatcgcgc acgcgtggca
     1201 gaaccacgtt gcacaagcga tcgagaaatt cgtacatctt gtcgccccgc aaagtaacgg
     1261 cgcagccgat gggcatgcct ttgcggattt taaagttggc gatggacttc ttggccttgg
     1321 tgatcatcgc tttctgtccg gtaatctgtc ctagttcctg cgcagcagta tccagcagct
     1381 tggcgttctg gctggcttcg cccaagccga tgttcacggt gattttctgc agcttcggaa
     1441 cggccatatt gttcttccag ccaaagcgct tcattagcgc cggcaccgtt tcgttgcgat
     1501 atttttcctg caatcgactc ttcattgcct tccttcgcct cgtgtcaaat tcaaattcgt
     1561 ttacttatcc agcgccgtgt tgcaacgccg gcaactgcga accttgctgc catccggcaa
     1621 aaccgtgtgt ccgatacgcg tatgcttgcc gcagctggcg cataccacca tcacgttgga
     1681 aacgttgatg gacgcctctt tttccacgat gccgcccttg atgtttttct gcggattggg
     1741 gcgcgtgtgg cgcttcatga tggccacatg ctccaccacc acgcgatttt tcttcgcgtc
     1801 gatggagagc actctgccgc tcttgcccgc atctttaccc gcgatcactc ttacgttatc
     1861 gcccttgcga atggcgataa tgtgccgctc agttctgtca tgcctcaaag tcatgagcta
     1921 ccaaacctcc ggagccaaag aaactatttt tgtgtactgg cgctcgcgca attcgcgagc
     1981 gaccggacca aacacgcgcg tgcccaccgg ctcgcccgcg tcgttgatca ataccgcggc
     2041 gttgtcgtca aaacggatgt acgtaccatc cttgcggcgc gcttccttgc gcatccgcac
     2101 caccacagct ttcacaactg tgccctgctt cactgcgctt tccggcgtgg cctcttttac
     2161 cgacgcagtg atcacgtcgc caagtccggc ctttaatccc gcatcgccgc ccagcggcaa
     2221 aatgcacatc agctttcgag cgccggaatt gtcggccacc gtcagccacg ttcgcatcat
     2281 gatgctcatc gagctgctcc ctgccca
//
LOCUS       DATCBB010000640         1001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_377222, whole genome shotgun sequence.
ACCESSION   DATCBB010000640 DATCBB010000000
VERSION     DATCBB010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377222"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VII37_07090"
     CDS             complement(<1..982)
                     /locus_tag="VII37_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069534.1"
                     /translation="MADAFRLGLSVDEVYALSKIDPWFLVQIEDLMRQEKSLAGRTLE
                     SLGADELRALKRSGFADARLARLLGTDTDAMRAYRHALGVRPVFKRVDTCAAEFATDT
                     AYMYSTYEEECEAQPTNNKKIMVLGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMV
                     NCNPETVSTDYDTSDRLYFEPLTLEDVLEVVAVEKPIGVIVQYGGQTPLSLAHGLEKN
                     GVPIIGTSPDRIDCAEDRARFQQMLFLENLRQPPNRTACSAEEGLAGVAEIGYPLVMR
                     PSNVLGGRAMEIIHAQVDLERYMRDAQEMSKTFPERMPILLDRFLDDAIEV"
BASE COUNT          201 a          326 c          264 g          210 t
ORIGIN      
        1 ccacctcgat cgcatcgtcc aaaaagcgat ccagcagaat cggcatacgc tccgggaaag
       61 tcttggacat ctcctgcgca tcgcgcatgt agcgttccag atcaacctgg gcatggatga
      121 tttccatcgc ccgcccgccc agcacattgg aagggcgcat caccaatgga taaccgattt
      181 ctgccacgcc tgcgaggcct tcctcggcgc tgcatgcggt acgattcggc ggctgcctca
      241 aattctccag aaacagcatt tgctggaagc gcgcgcgatc ttccgcacag tcgatcctgt
      301 ccggtgaggt gccaatgatg ggcacaccgt tcttttccag tccatgcgcc agactgagcg
      361 gagtctggcc gccgtactgc acgatcacgc cgatgggttt ttccaccgcc accacttcca
      421 gtacatcttc cagcgtcagc ggctcgaagt acaaacgatc cgaggtgtca tagtcggtgg
      481 acacggtttc cggattgcag ttgaccatga tggtttcata gccgtcttcg cgcatcgcca
      541 gtgcggcatg cacgcagcaa taatcgaatt cgatgccctg cccgattcga ttcggcccgc
      601 cgcccagcac catgattttc ttgttgttgg tcggctgcgc ttcgcattcc tcctcgtagg
      661 tggaatacat ataggcggtg tcggtggcga actcggcagc gcaggtatcc acccgtttga
      721 acaccggacg cacacccagc gcatggcggt aggcgcgcat cgcgtcggta tcggtaccca
      781 gcaacctggc cagccgcgca tcggcaaaac cgctgcgttt caatgcgcgc aattcatccg
      841 cgcccagact ctccagagtg cggcccgcca atgatttttc ctgacgcatc aggtcctcga
      901 tttgcaccag aaaccatggg tcgatcttgc tcaacgcgta cacttcatcc acgctcaaac
      961 ccaggcggaa tgcatccgcc accgcccaga tgcggtgcgg a
//
LOCUS       DATCBB010000641         1205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_53674, whole genome shotgun sequence.
ACCESSION   DATCBB010000641 DATCBB010000000
VERSION     DATCBB010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53674"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..>1205)
                     /gene="bshA"
                     /locus_tag="VII37_07095"
     CDS             complement(148..>1205)
                     /gene="bshA"
                     /locus_tag="VII37_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEY5069535.1"
                     /translation="GHDIHFISYAPPIRMNPNDPHTHFHEVEVASYPLFDHPPYTLAL
                     ATKMLEVFESEALDLLHVHYAIPHSVSAMLARSMAAPRRLPFITTLHGTDITLVGSNR
                     SYLPITRFSIEQSDGVTAISSYLQQQTVRDFEIKRPIEVIPNFVNCDLYTRKDDPKVK
                     AKWAPNGEPILMHLSNFRPVKRVTDAVEIFAMVREKMPAKLVLIGDGPDRGAAEYLVR
                     KKKLQKDVIFLGKQDQVYEKLGTADLFLLPSQLESFGLAALEAMACEVPVIATNVGGL
                     PEVVEHGVDGYLVEPGDVKRAGAYAIEILSRADRGREMGHTARLNAKQKFCANDVIPQ
                     YERYYQRVLESAAVATA"
BASE COUNT          306 a          391 c          251 g          257 t
ORIGIN      
        1 ttgcgccgcg cctatcgctg gatcgctgcc catcgcaaat gcgcagcagc aatctcttgc
       61 gccacacggc aaacagctca cgcaactaaa cactaattga ttttcagatt ctggaaaatg
      121 aactaacttg cagaagggaa atattaacta cgctgtcgcc actgccgctg actccaaaac
      181 ccgttgataa taccgctcgt attgaggaat cacatcattc gcgcagaatt tctgcttggc
      241 attcagcctc gccgtatgcc ccatctcccg cccgcggtcc gcgcgtgaca atatctcaat
      301 cgcatacgcg ccagcgcgtt tcacgtcgcc aggctcaacc aaatatccat ccacgccatg
      361 ttctacaact tcaggcagcc caccaacatt cgtagcgatc accggcacct cgcaagccat
      421 ggcctccaac gccgccaacc cgaatgactc aagttgcgat ggcagcaaaa acaaatccgc
      481 agtgcccagc ttctcgtaaa cctgatcctg cttgcccaaa aaaatcacat ctttctgtag
      541 ctttttcttg cgcaccaaat attccgccgc gccacgatcc ggcccatcgc caattaacac
      601 cagcttcgct ggcatcttct cgcgcaccat cgcaaatatt tccaccgcat ccgtcacccg
      661 cttcaccgga cgaaaattcg aaaggtgcat caaaatcggc tccccatttg gcgcccactt
      721 ggctttcacc ttcggatcat ctttccgcgt atacaaatcg caattcacaa aattgggaat
      781 cacttcaatg ggccgcttga tctcaaaatc ccgcaccgtc tgctgctgaa gataactcga
      841 aatcgccgtc accccatcgc tctgctcaat cgaaaaccgc gtaatcggca ggtagctgcg
      901 gttacttccc accagcgtaa tgtccgtccc gtgaagagta gtaataaacg gcagcctgcg
      961 cggcgccgcc atggaccgcg ccagcatcgc gctcaccgaa tgcggtatcg cgtaatgtac
     1021 atgcagcaaa tccagcgcct cggactcaaa cacctccagc atcttggtcg ccagcgccag
     1081 cgtgtaagga ggatgatcaa acagcggata agacgccact tccacttcat gaaaatgggt
     1141 atgcggatcg ttaggattca tacggatcgg cggcgcataa cttataaagt gaatatcgtg
     1201 cccac
//
LOCUS       DATCBB010000642         1182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_151489, whole genome shotgun sequence.
ACCESSION   DATCBB010000642 DATCBB010000000
VERSION     DATCBB010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151489"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1035)
                     /locus_tag="VII37_07100"
     CDS             complement(16..1035)
                     /locus_tag="VII37_07100"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HEY5069536.1"
                     /translation="MNISKGKQKGLEAIANSRGVIAALAIDQRSALRKLFTAATGIAE
                     EKIPRELLEQFKEAVSRILTPHASAILLDPEIGLSAATKRAKNVGLLLAYEQTGYDKS
                     VPGRLPRLLDGWSAAALAAAGANGVKTLLYYSPFSAPRINELKKDWVQRVGEECEAEG
                     APYFLELVAYHDELDEKGREFAALKPEVVTRGIEEFSKPQYRVDVLKVGVPVNMAFVG
                     GREKQIADAVYTREEAKEHFRRAAAAALKPFIYLSEGVSNELFSETLDLAVEAQANFS
                     GVLCGRATWKDGVPIFAAGGLPALEDWLANQGVKNIQRVNALLGSAKPWFSFHKMTDA
                     RVSPQ"
BASE COUNT          262 a          324 c          315 g          281 t
ORIGIN      
        1 tgcggttaga cgtggtcact gaggtgatac ccgagcatcg gtcatcttgt gaaaactaaa
       61 ccagggtttt gctgatccga gtagcgcgtt cactcgttga atatttttta ccccctggtt
      121 cgccaaccaa tcctcaagag cgggaaggcc cccggcggca aaaattggca cgccgtcctt
      181 ccacgtggca cggccgcaga gtacgcccga aaaattcgct tgggcctcga ccgcaaggtc
      241 gagagtttcg ctgaacaatt cgttgctgac gccttccgac agatagatga acggcttgag
      301 agcggctgcc gctgcacggc gaaaatgttc cttggcctct tcgcgagtgt agacagcatc
      361 tgcgatttgt ttttctcgac cgccaacgaa ggccatgttg acggggacac cgaccttaag
      421 gacgtcaacg cgatattgcg gtttggagaa ttcctcaatt ccacgagtca caacttccgg
      481 tttaagagca gcgaattcgc ggcctttttc gtccaattca tcgtgatagg cgactagttc
      541 aaggaaatat ggggcgccct ccgcttcaca ctcttcgccg acgcgctgca cccagtcttt
      601 tttgagttcg ttgatccgag gtgcactgaa tggagagtaa tacagcagcg tttttacccc
      661 attggcaccc gcggccgcca atgctgccgc ggaccagcca tccagaagtc gtggaagcct
      721 gccgggtacg gatttgtcat aaccagtttg ttcatatgcg aggagcagac cgacgttttt
      781 tgcgcgctta gtggcggcgg aaaggccaat ttccggatcg agcagaatgg cactagcgtg
      841 cggcgtcaaa atgcggctga ccgcctcctt gaattgttcc agaagttcac gcggaatctt
      901 ctcctcggcg atgccggttg ccgcagtaaa aagtttgcgg agggcgctgc gctggtcaat
      961 cgccaaagcg gcgatgacgc cgcggctatt ggcaatcgct tccaaaccct tttgttttcc
     1021 tttagaaatg ttcacatttt tccttggcta ctatcgcgaa aataaaggat aaagccttag
     1081 tgaattgatt ccacatcctc aaaccatgcg accgctccgc gctccagggc gggcaactta
     1141 aagaccaagc ggtctccgat acgttgtcca agcacggttt ga
//
LOCUS       DATCBB010000643         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_21185, whole genome shotgun sequence.
ACCESSION   DATCBB010000643 DATCBB010000000
VERSION     DATCBB010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21185"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VII37_07105"
     CDS             <1..546
                     /locus_tag="VII37_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HEY5069537.1"
                     /translation="MRLAPGFTRDFPAAVILVQHMPAAFTTQYATQLSEFTGVRVKEA
                     ENNESLIPGTFYICPGGQHLRITSGGRIQLDGNSGRINGYLPNIDITMESVAAFAGPL
                     SIGVILTGMGNDGTAGATAIKSAGGMVLAQDEATSVIFGMPAEAIKAGAVSQVLGIDD
                     IYPAIEKQVLAIARIATVGVR"
     gene            546..1091
                     /locus_tag="VII37_07110"
     CDS             546..1091
                     /locus_tag="VII37_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5069538.1"
                     /translation="MQFPAPSKSPALRTEQIILFRVSGQLFAVSSGSVQEVRSVDSLA
                     GSAAEISAPGLKKVRHIVRRGDKSLFVVNGALHFGLPVTSGLLVFVLRRTRTALLIDG
                     IEKMTTMTRLQALPQAFCNEERIWYRGVTALDQNVVPVVNPEGFLTSEEIALLEQSQT
                     LAASAVKAAAGSIGSIHEPAQ"
     gene            1075..1554
                     /locus_tag="VII37_07115"
     CDS             1075..1554
                     /locus_tag="VII37_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5069539.1"
                     /translation="MNPRNDLGSQSFVLLRLGDRRFAMIASQIAELVAPSRVFRFPHR
                     TERVEGVILRRGRIVPVCDVSEKLIGKNLLTRRFYLIAVRNYGEKSEWVALPVTGECE
                     LIATEMSPATEADAPHVAGWISYDGNVIEVLNIDALTPGPVPAPSVCVPLEMQEATT"
     gene            1551..>2170
                     /locus_tag="VII37_07120"
     CDS             1551..>2170
                     /locus_tag="VII37_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5069540.1"
                     /translation="MKTGRPPKILLIDSNVYFSKRVADALRKEGFEVIAASQAAFALT
                     TLEYDTPVAILCATNMREMGALEIANVVHADPKNAMLPIIAIGDGSQRALLESFQAGC
                     DDYIDRHRPPATIAAHVKQIIISKAEGFQPTQMLDQSNTSLSGSLAHHDLPGVMQMLG
                     QARQTGALHINAGEIDALMFFDAGEITHAESGNYFGDEAVIHILKSV"
BASE COUNT          480 a          604 c          600 g          486 t
ORIGIN      
        1 atgcggttgg ctcccggttt tacgcgagat tttcccgcgg ctgtaattct ggtacagcac
       61 atgccggccg ctttcaccac acaatacgcc acccagttgt ccgagttcac tggcgtgcgc
      121 gtcaaagaag ccgaaaacaa tgaaagcctc attcccggaa cattttacat ttgccccggt
      181 ggtcaacatt tgcgcattac gtccggagga cgcattcaac tcgacggcaa cagtggccgc
      241 atcaatggct acctaccaaa tattgatatc accatggaaa gcgtggccgc cttcgccgga
      301 ccgctgagta tcggggtaat cctaacgggc atgggaaatg acgggacggc cggcgccacg
      361 gccataaaat ccgccggcgg aatggtgctg gctcaggatg aagccacctc agtaattttt
      421 ggcatgcctg cggaagccat caaagctggc gcggtaagcc aggttctggg catcgacgac
      481 atttatcctg ccatcgagaa gcaggtcttg gcgatcgccc gcatcgcgac agtgggagtg
      541 cgctgatgca atttccagct cccagcaaat cgcctgcact gcgcacggag caaattattt
      601 tgttccgcgt gagtggacaa ttattcgccg tgtcctcggg atccgttcag gaagttcgca
      661 gcgtggacag cctggccggt tcagccgcgg aaatatccgc cccaggttta aaaaaggtgc
      721 gccacatcgt ccgacgcggt gacaaatcgc ttttcgtcgt gaacggagct ttgcatttcg
      781 gattgccggt gacgtccgga ctgcttgtat tcgtgcttcg ccgcacgcgc acggcgttgc
      841 ttatcgatgg catagagaaa atgaccacca tgacgcggct gcaggctctt ccgcaggcat
      901 tttgcaacga agaacggatc tggtatcgcg gggtcacggc gttggaccaa aatgttgtgc
      961 cggtcgtgaa tcctgaaggt tttttgacct ctgaagaaat cgcattgctt gagcaatctc
     1021 agactctcgc ggcttccgcc gtgaaagctg cagccggttc cataggatcg atccatgaac
     1081 ccgcgcaatg atctgggttc gcagtctttc gtgctgttac ggctgggaga ccggcgtttt
     1141 gccatgattg ccagtcaaat cgccgagctg gtcgcaccca gccgggtctt tcgctttccg
     1201 caccgaaccg agcgtgtgga gggtgtaatc cttcgtcgcg gccgcatcgt tcctgtgtgt
     1261 gatgtgtccg agaaactaat cggcaagaat ctgctgaccc gccgctttta cctgatcgct
     1321 gtacgcaatt acggagaaaa atccgagtgg gttgcgttgc cagttaccgg tgagtgcgaa
     1381 ctgatcgcca ccgaaatgtc tccggccaca gaagccgatg ctccgcacgt agccggttgg
     1441 atttcttacg acggcaacgt catagaggtt ttgaatatcg atgcgcttac gcctggacct
     1501 gttcccgcac catctgtatg cgtgccactt gaaatgcagg aggcgacgac atgaagaccg
     1561 gccgtcctcc taagattttg ctgatcgaca gcaacgttta cttttcaaag cgcgtagcag
     1621 acgcgttgcg gaaggaaggt ttcgaagtga ttgccgctag tcaggcggca ttcgccttga
     1681 ccactctgga atatgacacg ccggtagcga ttctctgtgc caccaatatg cgcgaaatgg
     1741 gtgctcttga aatcgccaac gtcgttcacg ccgatccaaa aaacgccatg ctaccgatca
     1801 ttgctattgg ggacggcagc cagcgcgcgt tgttggaatc ttttcaggcc ggatgcgacg
     1861 actatatcga cagacaccgt ccgcccgcga cgatcgcggc gcatgtgaaa caaattatca
     1921 tcagcaaggc cgaaggcttt cagcccacac aaatgctgga tcagtccaac accagcctca
     1981 gcggaagcct ggctcatcat gaccttcccg gtgtgatgca aatgctcgga caggcccgcc
     2041 agaccggtgc tctgcacatc aacgccggcg agatagacgc cttgatgttt tttgatgccg
     2101 gagaaattac ccacgcggaa agtggcaatt actttggcga cgaagcagtg attcacattc
     2161 ttaagagcgt
//
LOCUS       DATCBB010000644         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_119051, whole genome shotgun sequence.
ACCESSION   DATCBB010000644 DATCBB010000000
VERSION     DATCBB010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119051"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..581
                     /gene="folK"
                     /locus_tag="VII37_07125"
     CDS             78..581
                     /gene="folK"
                     /locus_tag="VII37_07125"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY5069541.1"
                     /translation="MESTKPEIVFLSLGSNVGDREKNLRTAIAALPELGVKLKKVSSI
                     YETEPVDLLEQPWFLNCVVEGETTVQPATLLRELRELERQMGSKKLVPRGPRLIDLDI
                     LVYGERTIDSPELQVPHPRMHLRRFVLAPLAEIAPDLKHPTWSGTAAQLLNGLSDQSL
                     VKKLARK"
     gene            complement(594..1223)
                     /locus_tag="VII37_07130"
     CDS             complement(594..1223)
                     /locus_tag="VII37_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069542.1"
                     /translation="MESTTPTQPETPRQVAILVLEEDADNAMAVRQLLDSEGWRVNIV
                     TGSDLLLNELRNGEWSLVVANAAVTGIDSAAFTMLRELASVPADEGGRIRVLFIVPEM
                     ADIKYMQALERVRLPYVVRPFNFHDLIQKVSDLLFEVRAIAVPLRQVGREFGVVRKKK
                     ADASRTNNSMFASRESYSYSEEEVAEYERQEASSSRRHKPRTNLGDPNG"
     gene            complement(1375..1893)
                     /locus_tag="VII37_07135"
     CDS             complement(1375..1893)
                     /locus_tag="VII37_07135"
                     /inference="COORDINATES: protein motif:HMM:NF021390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEY5069543.1"
                     /translation="MAGKFWATAASRSSITYGAFALVLLIAPSLRAQQPSPDPGPIPP
                     PSQPAPNSPTAPTAPVAPTNPPAGASPSSHMMRVWRQLKYTCDGDVKVVVNLHAKQAR
                     VVFKGHTYNLQQADESDGQKYTDGAVVWRDKDEVGALERSSKSGGNKSMAAGCHLQST
                     GTAPTTPAPSHP"
     gene            complement(1913..>3062)
                     /locus_tag="VII37_07140"
     CDS             complement(1913..>3062)
                     /locus_tag="VII37_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069544.1"
                     /translation="EGPEAWVYRAKDIATGADVTIHLAREVCVDAVATAVPETTPKAA
                     VSGKPATSAPSAAPSSTIAKNAFRIVLEHGQIGTLRGCARVAAELFPKIVNQSADDDD
                     PDKKKPPVTAITNFKAPTAIAFITSSGKIMVSRGAAKKIIPVTGTELSLSHDGKKLLY
                     TRNDSKMGSERTIVLYEVDAARSKDLIHGAVREAFWSPDDSRFAYLQSQDQKWRVLSA
                     PVSTPETATPLYSETVDSLQGGVDGHTVLANDAQSLYWIGDDRPQQSLPLKEIYGDAF
                     RVSDTDTIRICPVNPDLLLVSAKYFTAPAGAPADAAGIFLYEIRAKRRVVLTPPEQWS
                     SHGEWSRDGIQIFYTRRMSATSSVTYRIFWDGSGPRRYQDGTDLVVGQ"
BASE COUNT          646 a          800 c          940 g          676 t
ORIGIN      
        1 ctgccaaggc gccagcctag gctattttga ttttgctgcg cgaataatgc ggctgcctgc
       61 gaagccaacg gactatcgtg gaatcaacaa agcctgaaat tgtttttttg tcgctggggt
      121 cgaatgtcgg agaccgcgag aaaaatcttc gcacggcgat cgcggccttg cccgaactag
      181 gagtgaaact aaaaaaggtt tcctcaattt atgagactga gccggtggat ttgctggagc
      241 agccatggtt cttgaattgt gtggtggagg gagaaacaac ggtgcagcca gcaacgctgc
      301 tcagagagtt gcgcgagctc gagcggcaaa tgggcagcaa aaagctagtg ccgcgcggcc
      361 cgcggttgat cgatctggat attttagttt acggcgagcg gacgattgac tcgccggagt
      421 tgcaggttcc acacccgcgc atgcatttgc ggcgattcgt cctggccccg ttggccgaga
      481 ttgcgccgga cttaaagcat ccgacatgga gcggcaccgc ggcgcagttg ctgaacggac
      541 tttcggatca aagtttagtg aagaagctag ctaggaaata acgcgaaacg gaactacccg
      601 ttgggatcgc cgagattggt gcgcggtttg tggcgcctgc tggaactggc ttcctgccgc
      661 tcgtactccg cgacttcttc ctctgagtag gagtaggatt cgcgcgaagc aaacatgctg
      721 ttgttcgtgc ggctggcgtc cgctttcttt ttccgcacca cgccgaattc acggccaacc
      781 tgacgcagcg gaacggcaat ggcgcgcact tcgaatagca ggtcgctgac tttttgaatc
      841 aaatcatgga aattaaaagg ccgcacgacg tagggaaggc gcacgcgctc cagcgcttgc
      901 atgtatttga tgtcggccat ttccggaacg atgaaaagca cgcggatacg cccaccctcg
      961 tcggccggaa cggacgccag ttcccgcagc atggtaaacg ccgcgctgtc tatgccggtt
     1021 acggccgcgt tggccactac cagggaccat tccccgttgc gcagttcgtt taaaagtaga
     1081 tcgctgccgg tgacgatgtt gactcgccag ccttcggagt caagaagttg gcgaacagcc
     1141 atggcgttat cggcgtcctc ttcaagaacg agaatggcca cctggcgagg cgtttcaggc
     1201 tgggttgggg tagtgctttc cacctctcaa ttatagctgg tggataagcg cggcgatttt
     1261 gcaagagtac aggtgcggcg tatcggccaa tgaacaaggg tgcttacata gtctgaccgt
     1321 ttgtaccctc gaatagggcg ccgggtcagc gatttgaacc ctgataaatc cacatcacgg
     1381 atggcttggc gcgggcgtcg tgggtgcggt gccggtgctt tgcaggtgac agccggcggc
     1441 catagatttg ttgccaccgg atttggagga acgctcgagc gcaccaactt catccttatc
     1501 gcgccacacc acggccccat ccgtatattt ttggccatcg gattcgtcgg cctgctgcaa
     1561 attgtatgtg tggcctttga acacgacgcg agcctgtttt gcgtgtaagt tcaccaccac
     1621 tttaacgtcc ccgtcgcatg tgtacttgag ttgccgccaa acacgcatca tgtgggacga
     1681 aggagaagcg ccggcgggag gattggtcgg agcgacgggc gcggtgggag cagtaggaga
     1741 attgggggcc ggctgtgatg gcggcggtat ggggccggga tcgggcgaag gttgttgcgc
     1801 tcgaagcgac ggcgcgatca agaggaccag agcgaacgcg ccgtaagtga tcgatgatct
     1861 cgatgccgca gttgcccaaa atttgccagc catgatttgt tctcccaact tcttactgac
     1921 ccactactaa atcggttccg tcctgataac ggcgcggccc gctgccgtcc cagaaaattc
     1981 ggtaggtcac cgatgaggtg gccgacatgc gacgagtata aaagatctgt atgccgtcgc
     2041 gagaccattc gccgtggctg gaccactgtt ccggaggggt taaaacaaca cgccgcttgg
     2101 cgcgaatttc atagaggaaa attcctgctg catcggcggg cgcgccggcc ggcgcggtga
     2161 aatatttggc cgacaccaac agcaaatccg gattcacggg acagatgcga attgtgtccg
     2221 tgtccgacac gcgaaaagca tcgccgtaga tttctttcaa cggcaaagat tgttgcggcc
     2281 ggtcgtcgcc aatccagtac agactctgcg cgtcgttggc cagtacggta tggccgtcga
     2341 ccccgccctg cagcgaatca acagtctcgc tgtacagagg tgttgcagtt tcgggtgtgc
     2401 tgaccggggc agacaagact cgccatttct gatcctgcga ttgcagatac gcgaagcgcg
     2461 aatcgtccgg cgaccagaag gcttcacgca ctgcgccatg aatcaaatct ttggagcggg
     2521 cggcatcaac ctcgtagagc acgatggtgc gctcggagcc catcttggaa tcatttcgcg
     2581 tgtagagaag ttttttgccg tcgtgagaaa gggaaagctc ggtgcctgta acggggataa
     2641 tttttttggc cgcgccacgg ctgaccatga ttttgccgct ggaagtaatg aacgcaatcg
     2701 cggtgggggc tttgaaattg gtgatggcgg taaccggcgg ctttttcttg tcggggtcgt
     2761 cgtcatctgc gctttgattt acgattttcg ggaagagttc cgcggcaacg cgcgcgcaac
     2821 cgcgaagtgt gccgatttgt ccgtgttcca gaacaatgcg aaaggcattc ttggcgattg
     2881 ttgaagaggg cgcggctgat ggggcagagg tcgcgggttt tccggaaact gcagccttcg
     2941 gcgtcgtctc cggaacagca gttgctacgg catcgacgca aacttcgcgc gccaggtgaa
     3001 tggttacatc ggcgccagtg gcaatatctt ttgcgcgata aacccaagct tcaggtcctt
     3061 ca
//
LOCUS       DATCBB010000645         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_476526, whole genome shotgun sequence.
ACCESSION   DATCBB010000645 DATCBB010000000
VERSION     DATCBB010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476526"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1377)
                     /locus_tag="VII37_07145"
     CDS             complement(235..1377)
                     /locus_tag="VII37_07145"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY5069545.1"
                     /translation="MQVPLNGHSRKLRVGVLFGGRSGEHEVSLVSAASIIRGLDPQKY
                     EAVPIGISKEGHWLIGEGAQKMLPDVLRTGQRVVMSADPTESALMPIDGSARGQKLDV
                     VFPVIHGTFGEDGTMQGLLELAGLPFVGAGVLGSAIGMDKDVAKKLMQVAGIPVVPWI
                     AVQRADWERDPKAIRRAVEKKFKYPVFVKPATLGSSVGMTKVHSRAELGPALDLAAEF
                     AMKIMIERAVSAREIEVSVLGNHDPRASIPGEIVPHREFYDYAAKYLEEGTQLLIPAK
                     LKKSEIKKVQAMAVTAFRALELSGMARVDFFIEKRGGKIFLNEVNTIPGFTSISMYPK
                     LWEANGIPFRELITNLIDLALEQHREKARTKYQIELPEGASGALGG"
     gene            complement(1393..1839)
                     /locus_tag="VII37_07150"
     CDS             complement(1393..1839)
                     /locus_tag="VII37_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069546.1"
                     /translation="MTLFDPPADKSNKSRAMAFTIGALALFVVVLLYFAFRYYPERRA
                     AEHFFDALVAGDTDTAYRLWKPGPSYAMKDFMADWGPQGYYGPVKSYKILKVNAPKKA
                     NAIAVRVATSPFSPLPDAGDAEKSRKTRVVSVWVLTSDKSLSFPPE"
     gene            complement(1953..2126)
                     /locus_tag="VII37_07155"
     CDS             complement(1953..2126)
                     /locus_tag="VII37_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY5069547.1"
                     /translation="MAVRQDLLDILVCPLCKAPVKLLADGSGLKCQACKRVYPIRDDI
                     PVMLPEEAKIATD"
     gene            2328..>3056
                     /locus_tag="VII37_07160"
     CDS             2328..>3056
                     /locus_tag="VII37_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5069548.1"
                     /translation="MSVPSVSPLLAWAAGEKLKNWAGNIEYGTDRLYSATSVEQAQEF
                     VRKHARMKVLGTRHCFNHIADSTDEFLSLRPMNKVLELDAKARTVTIPANMSYGQLAP
                     YLHEHGFALHNLASLPHISVAGACATATHGSGEKNGNLSTSVSALEMITASGDIVTLS
                     RKQDGEKFLGAVVGLGALGVITKITLDLQPTFLMKQYVYENLPLSQMQEHFDTIEASG
                     YSVSLFTDWQKKRINEVWIKSRVEA"
BASE COUNT          689 a          801 c          848 g          718 t
ORIGIN      
        1 ggaggcgcac ctggccatgg aaatgatcgc ggatagcgac gccatggcgt ccatggaagt
       61 ggtggaaatt aatccggtga ttgacgagca caaccgcacg gctttgttgg gtgtggagtt
      121 gattctgtcc ggattagggc aaaaaattct gtaggactga acgttaaaac cggcgccgca
      181 agttgtccta attctcaaac gaaaactatc gtctgcgttg actcgccagc ctttttatcc
      241 gccaagtgcg ccgctggcgc cttccggtag ctcaatttgg tacttggtgc gagctttttc
      301 tcgatgttgc tccagcgcca gatcgatcaa gttggtgatt agttcgcgga aggggatgcc
      361 gttggcttcc cacagtttcg ggtacatgct gatggaggtg aaacctggaa tcgtgtttac
      421 ttcgttcaag aagatttttc cgccgcgctt ttcgataaaa aaatccacgc gtgccattcc
      481 gctcaactct agcgcgcgaa acgctgttac cgccatggcc tgtacttttt tgatttcaga
      541 ttttttgagt ttcgcgggaa ttagtaactg cgtgccttct tccaggtatt tcgccgcgta
      601 atcgtaaaac tcgcgatgcg gtacgatttc accgggaatg gatgctcgag gatcgtgatt
      661 gcccagaacg gaaacttcga tttcgcgagc acttacggcg cgctcgatca taattttcat
      721 ggcgaattcg gcggctaagt ccagcgcggg gccaagttcc gcgcgagaat gcaccttggt
      781 catgcccact gaggagccta gcgtcgcagg cttcacgaaa acgggatatt taaatttttt
      841 ctcgacggcg cgacggattg cttttgggtc ccgctcccaa tcagcgcgtt gcaccgcgat
      901 ccacggcact accgggatcc cggccacctg catcaatttt ttggcgacat ccttgtccat
      961 accaatcgcg gagcccaata cgcctgcgcc cacaaatggc aagccggcga gctcaaggag
     1021 accttgcatg gtgccatctt cgccgaaggt tccgtgtatc accggaaaga ctacatccag
     1081 tttttgtccg cgggcgctgc cgtcaattgg catcaacgcc gattcggtgg ggtcggcact
     1141 catgaccacg cgctgacccg tcctcaaaac gtcaggcagc attttttgcg cgccttcgcc
     1201 gatcagccag tggccttctt tggaaatgcc gatgggcacg gcttcgtatt tttgcggatc
     1261 gagaccgcga ataatggagg cggcggagac cagcgaaact tcatgttctc cggagcgacc
     1321 gccaaaaagg acgccgacgc gcagctttct tgagtgaccg ttcaatggga cttgcattcg
     1381 cctaggatgg cactattcag gcgggaaact taaagacttg tcgctggtga gcacccaaac
     1441 gcttactacc cgggtcttgc gacttttttc cgcatcgccg gcatctggca agggcgaaaa
     1501 cggactggtc gccactcgca cggcaatagc attcgctttt ttgggcgcgt taactttaag
     1561 aattttgtag ctcttcaccg ggccgtagta accctgcggt ccccaatcgg ccatgaaatc
     1621 tttcatggcg taactgggac ccggtttcca caagcgatat gcggtgtcgg tatcgccggc
     1681 gacgagcgcg tcgaagaaat gctcggcagc gcgtctttcc gggtagtagc gaaacgcaaa
     1741 atataaaagt acgaccacga aaagggccag cgccccaatc gtgaaagcca tggcacggga
     1801 tttattagat ttatcggcag gtgggtcaaa taacgtcatg gttttctcct gccccgcgag
     1861 cagtgaaaat cagtatagct taagcgcgta tttcttcggg ctaagcggcc gggaaaatcg
     1921 aacggggcgg gttaaactct aaaatcctgg aactaatcgg tcgcgatttt cgcttcttcc
     1981 gggagcatta ccgggatgtc atcgcggatg ggatagaccc gtttacacgc ttggcatttg
     2041 agccccgagc catctgccaa gagttttaca ggagctttgc aaagcgggca caccaaaata
     2101 tccagcagat cctgtcttac agccatggca acggctccta tcgtggaaaa tcggcgtgaa
     2161 tgcgacttta tcaaagcgac gctgcgcggt aaacccgcac ggccatgaca cggcagggaa
     2221 aaacattcag cctgggttca attgggttac gatactttcg gttttcgacg cggctaagca
     2281 gatcgactat gaataaaaga tctttcctga aacttgtctc cgctgcaatg tctgttccga
     2341 gtgtgtctcc gctactggcg tgggcggcgg gagaaaaatt aaagaattgg gctggcaaca
     2401 ttgagtacgg cactgaccgc ctttactcgg cgacttcggt tgagcaggcg caggagtttg
     2461 tcaggaagca tgcccggatg aaagtgctgg gcacgcgcca ttgcttcaat cacattgccg
     2521 atagcacgga cgaatttctg tcgctgcgtc cgatgaataa ggttcttgag ctggacgcga
     2581 aggcacgcac ggtgaccatc ccggcgaaca tgagctatgg gcagctggct ccttacctgc
     2641 atgaacacgg tttcgcgctg cacaatttgg cgtctctgcc gcatatttcg gtggcgggcg
     2701 catgcgctac cgcgacgcac ggatcggggg agaagaatgg caacctctcc acctcggtgt
     2761 ccgcgctgga aatgattact gcgtcgggtg acatcgttac gctttctcgc aagcaagacg
     2821 gcgagaagtt cttaggcgcg gttgtcgggc tgggcgcgct tggcgtgatc acaaaaatca
     2881 cgctcgatct tcaacctact ttcctgatga agcagtacgt ttatgaaaac ctgccgctca
     2941 gccagatgca ggaacatttc gatactatcg aagcaagcgg gtatagcgtc agccttttca
     3001 ctgactggca gaaaaaacgc atcaatgaag tgtggatcaa gagtcgcgtg gaagcg
//
LOCUS       DATCBB010000646         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_442114, whole genome shotgun sequence.
ACCESSION   DATCBB010000646 DATCBB010000000
VERSION     DATCBB010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442114"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..>2882
                     /locus_tag="VII37_07165"
     CDS             179..>2882
                     /locus_tag="VII37_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY5069549.1"
                     /translation="MVDAAPGIGIPGDLKIDLGIQPVSGRLAEVIYPTRRENLRGQME
                     YKTEVNFDRQEFWRARLILHSSQGDGESAANVEATPAGFGRWDLLLYLLPFAGVGFLW
                     FKAVTAKRNYRRKQLETGKPLLAVCVLAILCGAAWMLSSGCRGNKLPRKSSKEYVGFV
                     SIFYTGLAALQVGDDVRAESKLGQATQMAPGEPAGWADWGILALRQRNFDVAAQRLGA
                     ARDLAPKNDHIYYLLGLLESNRGRSAEAIADWRKSVELNPQNLRATYQLAEELERQGE
                     VGGADEFQKLVQNILGVQPNNLAALLELSRIAAKRGDATTLRAAVERIEKQSAGWAPE
                     AKQQLQALQAALAGQDTRPAATRTAFLRNVLMRDSTYRQSLGVIKAAAGEEAEPFTHF
                     LRLETPVFAPAAADTALTFETKLLEMVALDQGAKEHWDWIGSVSLSSEAPVTVIVANG
                     REVRLGTGAKLAFPGGTSNESPHPEGILPIDFNYDFKSDLVVAGAGGVRFWRQDSPEK
                     FTEVTAQTKLPGPIVNASYLGAWAVDIEADGDLDVVMGSKEGAPIVLRNNGDGTFTPI
                     HPFANVIGLQGFAWADFNGDGNPDAAIIDGAGGLHVFMNDRAGKFHERTLPDNPPHIK
                     TITVADANKNGVLDLLAVQEDGKIISLADKLGDSEKNDGSDWDSTVLAQVPDPGSNLA
                     GEVRLRVADLDNSGAFDLWLARTSAETGKTNTGALVWLADQNGKFALMAKPMGPPRSF
                     DAADLDSNGRMDLLGLSADGQAMRGVNRGTRNYHWQTIRPRARQATGDQRINSFGVGG
                     EMEIRSGLLVQKQPITGPQLHFGLGEQSGVDVARIVWPNGSVRAEFDLKADQQVVTEQ
                     RLKGSCPFLFAFDGHGMRFVKDAVPWSSALGLRINNTGTARIAAT"
BASE COUNT          622 a          701 c          922 g          637 t
ORIGIN      
        1 caaaatttca atgccggtgc tcgccgtctt catttcgctg tgcctttgcg tttccttcgc
       61 gccattggcg cgtgggcaca atggttctcc atttccgatc attgtcgatc agcgcgttgg
      121 gccgtgtatc gtttcgttgt ggacgcatcc ggatgttgga gttggaacct tttttgtcat
      181 ggttgatgcg gcaccaggga ttgggattcc cggcgatttg aaaattgatc tggggattca
      241 gccggttagc ggacggctcg ctgaggtgat ttacccaacg aggcgcgaaa atttgcgcgg
      301 tcaaatggaa tacaagaccg aagtaaattt cgatcgacaa gaattttggc gcgcgcgact
      361 gattttgcat agctcgcaag gtgatggcga gtccgccgcg aacgtggaag cgacgccggc
      421 gggatttggt cgctgggact tgctgctgta cttgcttcct ttcgcgggcg tgggtttttt
      481 gtggttcaag gcggttacag caaagcgcaa ctacagaaga aaacaactag aaaccggcaa
      541 accgctcctt gcggtttgcg tacttgcgat tttatgcggc gcggcctgga tgctttcttc
      601 cggatgcaga ggcaacaagt tgccgcggaa gtcttcgaag gaatacgtcg ggtttgtgtc
      661 cattttttat accggactcg ctgcgttgca agtgggcgac gatgttcgag ctgaaagcaa
      721 acttggacaa gccacgcaaa tggcacccgg tgagcccgct ggttgggcgg attggggaat
      781 actcgcgctg cgacagagaa actttgatgt tgcggcccag cggcttggag ctgcgcgcga
      841 tctcgcgccg aagaacgatc acatttatta cctcctcgga cttttggaga gcaatcgcgg
      901 gcgctccgct gaagccatcg ccgactggcg caaatctgta gagctcaacc cacaaaattt
      961 gcgcgcgacg tatcagttgg cggaagagct tgaacgtcaa ggtgaggtag gcggagctga
     1021 cgaatttcaa aagttggtgc agaacatctt gggggtccag cccaacaacc ttgctgcatt
     1081 gctggaattg agccgcatcg cggcaaagcg gggcgacgca accacgttac gcgccgcggt
     1141 ggaacggatt gaaaagcaat ccgcgggctg ggcccccgaa gcgaaacaac agctgcaggc
     1201 gttgcaagcg gcactggctg gccaggacac tcgcccggcg gcaacgcgca ccgcgttttt
     1261 gcgaaatgtc ttgatgcgcg attcgactta ccggcaaagc ctgggtgtta ttaaagccgc
     1321 ggcgggagaa gaggccgaac cgttcacgca ttttttgcga ctggagacgc cggtgtttgc
     1381 tcctgctgct gcggataccg cgctgacgtt cgaaacaaag ctgctggaaa tggtggcact
     1441 cgatcaaggc gcgaaagagc attgggattg gattggttcg gtgagcctca gcagcgaggc
     1501 gcctgttact gttatcgtcg cgaatggtcg ggaagtgcgg ttaggcaccg gagcgaaact
     1561 tgcttttccg ggcggcacat ccaatgaatc gccccatccc gaagggattt tgccgatcga
     1621 ttttaattat gactttaaga gtgacttggt ggtcgcgggc gctggtggag tgcggttctg
     1681 gaggcaggat agtcccgaaa agtttacgga agtgacggcg caaacaaaat tgccgggccc
     1741 gatcgtcaac gcctcgtact tgggcgcgtg ggccgtggat atcgaagctg acggggatct
     1801 ggatgtggtg atgggctcaa aggaaggtgc gccgatagtt ttgcgaaaca atggtgacgg
     1861 cacgtttaca ccgatccatc cgttcgcgaa cgtgatcggc ctacagggtt ttgcgtgggc
     1921 ggatttcaac ggcgacggaa atcctgacgc tgcgattatc gatggggcag gcggcttgca
     1981 cgtttttatg aacgatcgcg ccggtaaatt tcacgagcga acgttgccgg ataacccgcc
     2041 gcacatcaag accataactg tcgcagatgc aaacaagaac ggagttttag atttgttggc
     2101 agtgcaagag gacggaaaaa ttattagttt ggcggacaaa ctcggcgatt cagaaaagaa
     2161 cgatggatcc gattgggatt caacggtgct cgcgcaggtg cccgatccgg gcagcaatct
     2221 ggcgggcgaa gtacgattgc gagtcgcgga tctggacaac agtggggcgt tcgatttgtg
     2281 gttggcgcgc acttctgctg aaaccggaaa gaccaatact ggcgcgctgg tgtggctcgc
     2341 ggatcagaat ggaaagtttg cgctgatggc caagccaatg ggcccgccgc ggtcctttga
     2401 tgcggccgat ctggatagta atgggcggat ggatctgttg ggcctttccg cggatgggca
     2461 agcgatgcgc ggcgtgaatc gcggcacccg aaattatcac tggcaaacca ttcggccgcg
     2521 tgcaagacag gcgaccgggg accagcgcat taattcattt ggcgtcggcg gggaaatgga
     2581 aattcgctct gggttgctgg tgcaaaaaca acccattact ggaccgcaat tgcactttgg
     2641 cctgggcgaa caaagcggcg tggatgtggc ccgcattgtg tggcctaacg gatcggtgcg
     2701 tgcggaattt gatctgaaag cggatcagca agtggtcacc gagcagcgac tgaaaggttc
     2761 ttgtccattt cttttcgcgt ttgacgggca cgggatgcgc ttcgtgaagg atgcggtgcc
     2821 gtggagctcg gcactgggcc tgcgtatcaa caacaccgga acggctcgca tagccgcgac
     2881 cg
//
LOCUS       DATCBB010000647         1634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_215855, whole genome shotgun sequence.
ACCESSION   DATCBB010000647 DATCBB010000000
VERSION     DATCBB010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215855"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VII37_07170"
     CDS             <1..288
                     /locus_tag="VII37_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069550.1"
                     /translation="QLVVLYGARTPDDFCYRDETQSWEDAGVELRQVISKPDGHDWSG
                     PTGYVQTLLDHVLPDLNSPVALVCGSREMIAQTRDRLQQMGFAPEAILTNY"
     gene            complement(512..1582)
                     /locus_tag="VII37_07175"
     CDS             complement(512..1582)
                     /locus_tag="VII37_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5069551.1"
                     /translation="MTQAQLALQNTQTGETQFPMAHRWKTFAGLSILVIITLVVVGIT
                     FTIGWRPIIGAKKRALTDRKFEATPQRLARGKYLVDGVMGCFSCHTDADWSKPGAPPV
                     AGREGSGHVRSDQDTPWLIAPNITPDKETGAGNWSDDTLARAIREGIGHDGRALFSVM
                     PYPNYRQMSDEDLASVIVYVRSVPAVRNQLPTTRMPFSLNFFIQNVPEPVTAAVPAPD
                     QSTPVARGTYLVRMAACANCHTPQEKGQPLPGMDFAGGFLLKEPKGDVVSNNITPAAS
                     GIGYYNDTSFVQALRTGKVGARPLHASMPWYFFGKMSDDDLKSIFAFLQTLKPVRHQL
                     DNTEPPTYCRLCKQKHGFGHTN"
BASE COUNT          326 a          450 c          474 g          384 t
ORIGIN      
        1 caattggtag tgctttatgg cgcgcgcacg cccgacgatt tctgctatcg cgacgagacg
       61 caaagttggg aagacgccgg cgtggaactg cgtcaggtaa tttccaaacc cgatggccac
      121 gactggtccg gtcccaccgg ctatgtgcag acgctcctgg atcacgtcct gcctgatttg
      181 aattctcccg tggctttggt ttgcggttcg cgcgaaatga tcgcgcagac gagagatcgt
      241 ttgcagcaga tgggctttgc gcccgaggcg atcctgacga actattgact tggcagtagc
      301 gttttccgcc gtgatgtatc cacagattgc gcagattaca cggataagaa cgtaagcaaa
      361 cagtttcatg gcagcgcaga gtgacccttc tacttatcgg cctgtctgcg aaatctgcgt
      421 aatctgtgga tcactttttg tggctaacag tcttgcgtgt tctgatccgt gctaatccgc
      481 gttcatccgc ggctggactt ggctgtgccc ctcaattcgt gtggccaaac ccatgcttct
      541 gtttgcacag acgacagtag gtcggaggtt cggtgttgtc taactgatgc ctgaccggct
      601 tcagcgtttg cagaaacgca aagatggatt tcagatcgtc gtcagacatc ttgccaaaga
      661 agtaccacgg catcgatgca tgcagcgggc gcgcgccgac ttttccggtg cgcagggcct
      721 gcacaaaaga agtgtcgttg tagtaaccga tccccgacgc ggcgggcgtg atgttgttgg
      781 aaactacatc acccttcggc tccttcaaaa ggaaaccacc cgcgaaatcc attccgggca
      841 gcggctggcc tttttcctgc ggcgtgtggc aattagcgca cgctgccatc cgcaccagat
      901 aagtaccgcg agcgaccggc gtcgattgat ccggcgccgg caccgcggca gtcaccggtt
      961 cgggaacatt ttggatgaaa aagttgagcg agaaaggcat cctggttgtc ggcaactgat
     1021 tacgcaccgc gggcaccgag cggacataga cgatcactga cgccagatct tcgtccgaca
     1081 tctggcgata atttgggtaa ggcatgacgg aaaagagcgc ccgtccgtca tggccgatgc
     1141 cttcgcgaat ggcgcgcgcc agcgtgtcgt cgctccagtt gccggcacca gtttctttgt
     1201 cgggcgtgat gttcggggcg atcagccagg gcgtgtcctg atcagaccgg acgtggcctg
     1261 agccttcacg cccggcgacc gggggcgcgc ccggctttga ccaatcggca tcggtatgac
     1321 aactaaagca gcccatgacg ccgtcgacca ggtacttacc gcgggccagt ctttgcggcg
     1381 tcgcttcaaa ttttcgatcc gtgagcgcgc gtttcttcgc gccgatgatg ggacgccagc
     1441 cgatcgtgaa ggtgattcct acgactacca gcgtgatgat tacgagtatg gataggccgg
     1501 caaatgtttt ccaacgatgc gccatgggga attgagtttc tcctgtttga gtattttgta
     1561 acgcaagctg cgcttgcgtc accttcaatc aatctttttc cattcgtgtt cgtgtgattt
     1621 cgtggatcgt cctg
//
LOCUS       DATCBB010000648         1013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_411055, whole genome shotgun sequence.
ACCESSION   DATCBB010000648 DATCBB010000000
VERSION     DATCBB010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411055"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VII37_07180"
     CDS             complement(<1..976)
                     /locus_tag="VII37_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069552.1"
                     /translation="MDASLAVLARLFPIETAGRMYLSLCVLALPGAAWFFLRQAQPDA
                     EAASLWALLIAYNVFFLEGFLNFDLSIAMGFLALGLWLRWLAKPETGRWIAALVAFTA
                     LYFTHLLGFAIAGLIVVAYLVLSRRHVRDWLWCGALAVPGVAFYLHSSRVGLSANKII
                     FHGWTDKLDSLGMILHGYWPLLDWISLVALAVWFLAAWWRNPEFRWDQKWIAIAVFFF
                     ALFWVIPWMWGQEASDLDIRVLPFLFVAILAMARVGRRAKALATIPLLLFAARTVSVT
                     RHFKEAQPALAGLARSFDAVPRGALVLPIVEGDEDPIERPFTHFWAYGV"
BASE COUNT          275 a          329 c          267 g          142 t
ORIGIN      
        1 tcactccgta ggcccagaaa tgcgtgaagg gacgctcgat ggggtcttcg tctccctcca
       61 cgatgggcag cacgagcgcg ccgcgcggca cggcgtcgaa cgaacgcgcg aggccagcca
      121 atgccggctg tgcttcctta aaatgccgcg tcacgctcac ggtccgcgcc gcgaaaagta
      181 aaagcggaat cgtagcgagc gcttttgcgc gccgccctac gcgcgccatg gcaaggatcg
      241 caacaaacaa aaaaggcagg actcgaatat cgaggtcgga cgcttcctga ccccacatcc
      301 acgggatcac ccagaacaac gcaaagaaaa acacggcgat ggcgatccat ttttgatccc
      361 agcgaaattc gggattgcgc caccacgccg ccaaaaacca taccgccagc gcgaccagcg
      421 aaatccaatc cagcaggggc caatagccgt gcaggatcat gccgagcgag tccagcttgt
      481 cggtccatcc gtgaaagatg atcttgttcg cgctcagccc cacacgcgag gagtgcaaat
      541 aaaacgccac gcccggtacg gccaatgcgc cacaccacag ccagtcacga acgtgccgtc
      601 tcgaaagcac gaggtatgcc accacgatca atcctgcaat ggcaaaaccg agcaggtgcg
      661 tgaaatataa cgccgtgaat gcaacgagtg ctgcaatcca ccggcctgtt tccggcttcg
      721 caagccatcg cagccataaa ccaagcgcca aaaatcccat cgcaatgctc aaatcgaaat
      781 tcagaaatcc ttcaaggaaa aagacgttgt acgcgatcag cagcgcccac aaagatgctg
      841 cctccgcatc cggctgcgcc tggcgaagaa aaaaccatgc cgcgccagga agcgcgagga
      901 cgcacagact aaggtacatg cgccccgccg tctcaatcgg aaaaagccgc gcaagcacgg
      961 cgagcgaagc gtccatcccc aaatagggat acgcgcccca atccgcgcgg tag
//
LOCUS       DATCBB010000649         1164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_247574, whole genome shotgun sequence.
ACCESSION   DATCBB010000649 DATCBB010000000
VERSION     DATCBB010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247574"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1164
                     /locus_tag="VII37_07185"
     CDS             <1..>1164
                     /locus_tag="VII37_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5069553.1"
                     /translation="RAVRDAAKSADKEVELDLGGSETELDNSIIQQISDPLVHLVRNS
                     VAHGIESSADRIAAGKPQLGKVSLRAYHRGNHIYIEVQDDGRGIDYDRVRNSAIERGL
                     VSSETADRLTERDLREMLFHPGFSTAPVKTELAGRGVGLDVVRANLNTLNGEIDIQSI
                     TGLGTTFTLKVPLTLIISPALFVRCGATSFALPLAVVEEIRRLRSDEIENVGGKLLTK
                     VRDVVTEVVRLDGYLGLPPLEAINGYFHMVVANTGNRQIGLVVEEVLGKDEIVIKNLG
                     EYLRRVKLFPGTTIAPDGSLILLLDLNRMVATDPTERRTLQASASAARIFAPGSAAVA
                     HGTIPAEAIDRVTHERLIVVADDSISVRKFVGRMLEKNGYVVKLAADGLEAAEL"
BASE COUNT          251 a          324 c          337 g          252 t
ORIGIN      
        1 gcgcgcggtt cgcgacgccg caaagtccgc tgacaaagag gtagagctcg atttaggtgg
       61 cagtgaaacg gagctggata acagcatcat ccagcaaatt tccgacccgc tggtccacct
      121 ggtgcgcaac tcggtggcac acggtatcga atccagcgcg gatcgtattg cagccggtaa
      181 gccgcagctc ggcaaagtca gcttgcgcgc ttatcaccgc ggaaatcaca tttacatcga
      241 agtgcaagat gatgggcgcg gcatcgacta cgaccgcgtg cgaaatagcg ccattgagcg
      301 cggactagtc tcctcggaaa cggctgatcg cctcactgag cgcgatctgc gcgaaatgct
      361 tttccatcct ggattctcaa ccgctccggt caagaccgag cttgcaggac gcggcgttgg
      421 cttggatgta gttcgcgcca atctcaatac cctcaacggc gaaatcgata ttcaaagcat
      481 cactggattg ggcaccactt tcactctgaa agttcctttg accttgatca tctcgcccgc
      541 gctattcgtg cggtgcggtg cgactagctt cgccttgcct ttggcagtgg tcgaggaaat
      601 tcgacgcctg cgctccgacg agatcgaaaa tgtcggcgga aagctactca caaaagttcg
      661 cgatgtggtc acggaagttg tgcgcctcga tggctatctg gggttgccgc cgctggaagc
      721 catcaacggc tatttccata tggtggtagc caacaccggc aaccgacaga tcggtttagt
      781 ggttgaagaa gtcctcggca aagatgaaat cgtcatcaaa aatctgggcg aatatctgcg
      841 gcgcgtcaaa ctttttccgg gaacgaccat cgctcctgac ggcagcttga tccttttgct
      901 ggatttgaat cgcatggtcg ccactgatcc taccgagcgc cggactttgc aggcctcggc
      961 gagtgctgcg cgcatatttg cgccgggctc cgcggctgtg gctcacggca ctatccccgc
     1021 tgaggctatc gatcgtgtaa cgcacgagcg gctcattgtg gtggccgacg attccatcag
     1081 cgtgcgcaag tttgtcggcc gcatgttgga aaagaacggc tatgtcgtga aactggcagc
     1141 cgatggactg gaagcagcgg agct
//
LOCUS       DATCBB010000650         1236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_21316, whole genome shotgun sequence.
ACCESSION   DATCBB010000650 DATCBB010000000
VERSION     DATCBB010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21316"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VII37_07190"
     CDS             complement(<1..468)
                     /locus_tag="VII37_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069554.1"
                     /translation="MRKLQVGCLPLILCGASFLLGQTSATSSPVVERSVTPSSATSPT
                     ASRAHNRISLDVLVTDKAGKAVSDLEPFDFSILDNNQPRKVLGFRRTDGKIGSKVDPP
                     VELIIVADAVNMAYQGVTLMRLEVEKFLRRDGGHLAQPTSVFIFTSEGMRIQPQ"
     gene            complement(527..>1236)
                     /locus_tag="VII37_07195"
     CDS             complement(527..>1236)
                     /locus_tag="VII37_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069555.1"
                     /translation="LAQPVSLILLTDLELRMQPRPSTDGKAVASVLDQIKGRVSHITP
                     AMGGEGALERFQLSARQMGNIAENEARKPGRKLLIWIGPGWPLVTRTELGYYSAREQR
                     RYFDGIVQLSTKLREARMVVYSVAPANIGAGGGNSLNTLRYKDFLKGVKSASEADSGN
                     LSLKVLVTQTGGQILGPDNDVSGQIDRCIADADLFYRISFDPHLGGHADEYHELNVAV
                     GKPGLTVRTNTGYYGQP"
BASE COUNT          261 a          410 c          300 g          265 t
ORIGIN      
        1 ctgcggttga atcctcatcc cctcgctggt aaaaataaat accgacgtgg gctgtgcgag
       61 atgaccgccg tcgcgccgca gaaacttctc cacctctagc cgcatcaggg ttaccccctg
      121 gtacgccata ttcactgcat ccgccacaat gatcaattca accggaggat cgaccttgct
      181 tccaatcttt ccgtccgtgc gccgaaaacc aagaaccttt cgcggctgat tgttgtccag
      241 aatgctgaaa tcgaacggct ccagatcgct cacggcctta cccgccttat cggtaacaag
      301 cacatcgagg ctgatgcgat tgtgcgctct gctcgcggtc ggcgaggtcg cgcttgaagg
      361 ggtaacactc cgctctacaa caggtgacga tgtcgcgctt gtctgcccca acagaaaact
      421 cgccccgcac aaaatgagcg gcagacaccc tacttgcagc ttgcgcacgc aaactcccct
      481 tcctaacaat gctttacttt cctcgcggaa gtgtacaacc tccgctttac ggctggccgt
      541 aatatcccgt attggtgcgc accgtcagtc ccggcttgcc caccgccaca ttcagctcgt
      601 gatattcgtc ggcgtgcccc ccaagatggg gatcaaacga aatcctgtag aacaaatcgg
      661 cgtccgcgat gcagcgatcg atctggcctg acacatcgtt gtccgggccc aggatctggc
      721 cgcccgtctg cgtcaccagc accttcagcg acaggttgcc ggaatccgct tcgctcgcgg
      781 acttaacccc cttcaaaaaa tctttgtagc gaagcgtatt caaagaatta ccgccgcccg
      841 ccccaatatt tgccggagct acgctgtaaa caaccatgcg cgcctcccgc aattttgttg
      901 aaagctggac tatcccgtcg aagtaccgcc gctgctccct tgcggagtag tatccaagct
      961 cggtccgcgt cacaagaggc cagcctggac ctatccagat cagcagcttt cggcctggtt
     1021 tccgcgcctc gttttcggca atgtttccca tctggcgcgc cgaaagctga aagcgctcca
     1081 gcgctccctc cccacccatc gccggagtga tgtgactaac cctccccttg atctgatcca
     1141 gcaccgacgc caccgctttg ccgtctgtcg aaggccgcgg ttgcatgcgc aattcgagat
     1201 cggtaagcag aatcaatgag accggctgtg caaggt
//
LOCUS       DATCBB010000651         2042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_345506, whole genome shotgun sequence.
ACCESSION   DATCBB010000651 DATCBB010000000
VERSION     DATCBB010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345506"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /gene="hpnC"
                     /locus_tag="VII37_07200"
     CDS             complement(<1..1817)
                     /gene="hpnC"
                     /locus_tag="VII37_07200"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY5069556.1"
                     /translation="MKEAQKYTRWLATHHYENFNVASWLLPAELHQHFYNLYAYCRWA
                     DDLGDEIPEPARALQLLNWWEHELNACYDGRPAHPVFVALRETIVAKDIPEQEFADLL
                     KAFRQDQKIKRYPNWSTLLHYCEYSANPVGRLVLYLCGYRDEHRQRLSDATCTALQLA
                     NFWQDVSRDMEKDRIYIPLDEAAAHGITEKDILERNFDERYISLMKSLIARTRTLFAE
                     GAPLAKIVDARLSVDLELFSKGGIAVLDAIEAMGYDTLHRRPSISKTKQVRLLGRALV
                     TRAVATNRKPDDLQNSIAVSSLADSYQECQRIIKAAHSNFYYAFFLLPKPKRDALTAL
                     YAFMRLVDDVADEGDNVADKQRGLAKWRAAFDRALTQRTTDVPLDDEPNGVSVLPALV
                     DTMQRYSMPSRYLHDLISGAEMDLTVRTYPTFERLKEYCYRVAGTVGLTCTHVFGFRD
                     SHALDLAEKLGLAFQLTNIIRDVHEDYKLGRIYIPAEDLARYNVSSADFGLSHATLGV
                     RELLRFEAERAWLLYKQGAELLSLIDEDSRGTLWLLTHTYSALLARIESQDFAVFGER
                     VSLSRAEKMVFIAKARFGRLTEENIIEKRDRDRRRPGGTG"
BASE COUNT          455 a          498 c          638 g          451 t
ORIGIN      
        1 ccagtcccgc caggccgccg ccgatcacga tcgcgctttt caataatatt ctcttccgtc
       61 aggcggccga agcgcgcctt ggcaataaac accatttttt ccgcgcgcga aaggctcacg
      121 cgttcaccga acacggcaaa atcctgagac tcgatgcgcg cgagaagcgc gctgtaggta
      181 tgagtaagca gccataaggt gccacggctg tcctcgtcga tcaggcttaa tagctcggcg
      241 ccctgtttat atagcagcca ggcacgctcg gcttcgaaac gcagcaactc gcgcacgcca
      301 agggtggcgt ggcttaaacc gaaatcggcg gatgatacgt tgtagcgagc caaatcttcc
      361 gcagggatat agatgcggcc caatttatag tcttcgtgaa cgtcgcgaat aatattggtg
      421 agttgaaagg cgagtcccag cttttccgcg aggtccagcg cgtgcgaatc gcgaaagcca
      481 aagacgtggg tgcaggtcag gccgacagtg ccggcgacgc gataacaata ttctttgagg
      541 cgttcgaagg ttgggtaagt gcgtacggtt aaatccattt ccgcgccgga aattaaatca
      601 tgaagatagc gggaaggcat gctgtaacgc tgcatggtgt cgacgagcgc gggtaggacg
      661 gagacgccgt tgggttcgtc atcgagcggc acgtcggtgg tgcgctgggt tagggctcgg
      721 tcgaaagcgg cgcgccattt ggctagtccg cgctgcttgt cggcgacatt atcgccttcg
      781 tcggctacat catcgactag gcgcatgaag gcgtatagag cggtgagcgc gtcacgcttt
      841 ggtttcggca agaggaaaaa cgcgtaataa aaattgctgt gcgcggcttt gatgatgcgt
      901 tggcattcct gataggaatc cgcgagagag ctgaccgcga tggaattttg cagatcgtcc
      961 ggtttgcgat tggtcgctac cgcgcgtgtt accaaggcgc ggccgagcaa gcgcacttgc
     1021 ttggttttgc tgatagaggg gcgacggtgc agggtgtcgt agcccattgc ctcgatggcg
     1081 tcgaggacgg cgattccgcc tttgctgaag agttcgaggt ctacgcttaa gcgggcgtcc
     1141 actatttttg ccagcggcgc gccttcagcg aacaaggtgc gggttctggc gatcagagat
     1201 ttcatgagcg agatgtagcg ttcgtcgaag ttgcgctcga gaatatcttt ttcggttatg
     1261 ccgtgagcgg cggcctcgtc gagtggaatg tagatgcggt ctttttccat gtcgcgagaa
     1321 acgtcttgcc agaaattggc gagttgcagc gcggtgcagg tggcgtcaga aaggcgttgg
     1381 cggtgctcat cacggtatcc gcagagataa agaaccaggc ggccgacggg attggcggaa
     1441 tattcgcagt agtgcaggag cgttgaccag tttggataac gcttaatttt ctggtcctgg
     1501 cgaaaggctt tcaatagatc ggcgaattcc tgttcgggga tatccttggc gacgatggtt
     1561 tcgcggaggg ccacgaagac ggggtgcgcg ggacggccat cgtagcaggc gtttaactca
     1621 tgttcccacc aattcaataa ttggagcgcg cgggcgggct ccggtatttc gtcacccaaa
     1681 tcgtcggccc agcggcaata ggcatacaaa ttgtaaaaat gctggtgaag ctcagcgggc
     1741 agaagccagg aagctacatt gaaattttcg tagtggtggg tggcgagcca gcgcgtgtat
     1801 ttttgagctt ctttcacgga gcagccggag gcgggtgctc gatcggcgat gtgcaattcc
     1861 ccaggacaca gcatacagcg aataaacctc acgatgactg ctcaagtcta ggaaaaaatt
     1921 ccggcgattc gattttttca gatgccggaa caattacacc cgaaacagaa tgaatcgcgc
     1981 actattgcga tggtggcgcg gcaataataa ttacggcccg acaaaattat gggcgaacgg
     2041 cc
//
LOCUS       DATCBB010000652         1381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_377592, whole genome shotgun sequence.
ACCESSION   DATCBB010000652 DATCBB010000000
VERSION     DATCBB010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377592"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VII37_07205"
     CDS             <1..507
                     /locus_tag="VII37_07205"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5069557.1"
                     /translation="AAERAGFFVDYQAQTHSGAALANELGEKLRGQTIFLPRSDRANP
                     DLPQLLRNYGAEVTEVIAYRTVTPVNLDEEKIAAIVNGEVEAILFFSPTAVEHFVGIV
                     GKENLRNLQNQSAITAVGPITANALRQAGADTLLVAEDTTADAVIAALERHFAGPQKA
                     SAAGVVHE"
     gene            504..>1381
                     /gene="hemB"
                     /locus_tag="VII37_07210"
     CDS             504..>1381
                     /gene="hemB"
                     /locus_tag="VII37_07210"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY5069558.1"
                     /translation="MSFPTHRGRRLRRNEALRGLVRETRLSAAGLIYPMFVCPGQKVR
                     QEVSSMPGVFQQSADQIVQECREVESLGIPGVILFGLPEKKDARGMSSVAADGVVQRA
                     IEAIRKAKLKLLIITDVCLCEYTDHGHCGVIEDGEVANDATLDILAEQAVSHARAGAD
                     IVAPSDMMDGRVGVIREALDEHKFENISILAYAAKYSSGFYGPFREAAQSTPQFGDRR
                     GYQMDPANAREALREVEMDLDEGADMVMVKPALPYLDIIRRVRDEFDVPVAAYNVSGE
                     YSMVKAAVQKGWLDEQR"
BASE COUNT          328 a          356 c          420 g          277 t
ORIGIN      
        1 gcagcggaac gcgcaggttt ttttgtggat taccaggcgc agactcacag tggagcagca
       61 ctcgcgaatg aactgggaga gaaactgcgc ggccaaacaa tatttttacc gcgaagcgac
      121 cgcgccaatc cggatctgcc acagcttttg agaaactatg gtgcggaagt gaccgaggtt
      181 atcgcgtacc ggacagttac cccggttaat ctcgacgaag aaaaaatcgc ggccattgtg
      241 aatggtgaag ttgaggcgat tttatttttt agtcccaccg ctgtggagca cttcgttgga
      301 atcgtgggta aagagaatct ccgcaatttg caaaaccaat ccgcaatcac ggctgtggga
      361 cccataacgg cgaatgcgtt gcgccaagcg ggtgccgata ctttgctggt ggctgaggat
      421 accaccgcgg acgcggtcat tgcggcgctt gagagacatt tcgcaggccc acagaaagcc
      481 tcggctgccg gagtagttca cgaatgagct ttccgacaca ccgagggcgg cgattgcgcc
      541 gcaacgaagc cctgcgcggc ctggtgcgcg aaacgcgact gagcgctgcc gggttaatct
      601 atccgatgtt tgtatgtcca gggcaaaaag tgcggcagga agtcagctcc atgcctggag
      661 tgtttcaaca atcggccgat cagattgtcc aggaatgccg cgaagtcgag tcgctgggta
      721 ttccgggcgt aattctattc gggctgccgg agaaaaaaga cgcccggggc atgagctcgg
      781 ttgccgccga tggtgtagtg caacgcgcga tagaagccat ccgtaaggca aaactgaaat
      841 tgctgatcat caccgatgtt tgcctgtgtg aatataccga ccacgggcat tgcggcgtaa
      901 tcgaagacgg cgaagtggcc aacgatgcca ctcttgatat tcttgccgag caagccgtct
      961 cgcacgcccg agccggagcc gacatcgtgg ctccatccga catgatggac ggacgcgtcg
     1021 gtgttattcg cgaagcgctc gacgaacaca aattcgagaa catttcaata ctcgcgtatg
     1081 cggcaaaata ctcctctgga ttctacggac cctttcgcga agccgcgcag tcaacgccgc
     1141 agtttggcga ccgccgcggt taccagatgg atcccgcaaa tgcccgcgaa gcattgcgag
     1201 aagtggaaat ggatttggac gaaggtgcgg acatggtgat ggtcaagccc gctctgccgt
     1261 atctcgacat catcaggcgc gtacgcgatg aattcgatgt gccggtggca gcgtataacg
     1321 tgagtggaga gtactcgatg gtgaaggccg cggtgcaaaa gggatggctg gacgaacagc
     1381 g
//
LOCUS       DATCBB010000653         4386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_85672, whole genome shotgun sequence.
ACCESSION   DATCBB010000653 DATCBB010000000
VERSION     DATCBB010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85672"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="VII37_07215"
     CDS             complement(<1..1441)
                     /locus_tag="VII37_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY5069559.1"
                     /translation="MSERTELSPTRVLRFGVFQVNLAARELRKHGVRIRLPGQPFRVL
                     SILLERPGQVVSREEMRDKLWDSETFVDYERSLNSAIRKLRAVLGDSQESSRYIETVP
                     RLGYRFIAPVEQVPSLAETPRASVLEPPPSAPKVVRPPARRGSLILGICAILLTLTVS
                     YFTWSRMQARPQPSAERLMLAVLPFENLTGDPAQEYLSDGLTEEMIAQLGRLDPEHLG
                     VIARTSVMHYKHTTEAAGQIGRELGVQYVLEGSLRREADRVRITAQLVQMKDQTHIWS
                     RQYDRELKSLLALQGEIAQETADEIQLRLGQGHKLRAADRKLASLTSYETYDLYLKGR
                     YFWNKRTKDGFQQAADYFQQSIASDPNSARAYAGLADSYALMSSWSFVPQTEAIPKAR
                     AAALRAMQLDVSLAEAHTSLALIAENYDYDWQTAEKEYRRAIQLDPQYATAHQWYAEC
                     LSFQGRFSEALEESERARQLDPLSLIIATD"
     gene            1658..3244
                     /locus_tag="VII37_07220"
     CDS             1658..3244
                     /locus_tag="VII37_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069560.1"
                     /translation="MRTISARRSGFSRRLYPFASLSSQSKRAFVSVTILTVAMLMAMS
                     AISRQPLNFIPNGTPFRDSRGASETYSTVGGGIDQTGPFFQSMGTNGRSCATCHQPSD
                     GMSIAAEEVQERFFMTRGRDPIFRTVDGSNCNHNIDVSTLEGMYDAYSLLRTRGLIRI
                     PIAIPATADYQVVSVQNQYGCNESDVVSMYRRPLPTTNLRFLSAVMFDGRESTPLTGT
                     MKIADANYPTSLVSNLIHQSVDATTIHAQGDGTRPTPAEQQEIVNFEMALFTAQTFGD
                     DIGPLNTHGANGGPQPLITQPFFISINSSVHPIVQTLELPAGLVTRGDNIFTPAIFDT
                     FGAWSEFPEHSRRAAVARGEAIFNSRAINITGVAGINDPAGAGGLLPNGVQAFQGTCG
                     TCHDTPNVGNHSFPTPLNIGTGDPSPTNPSANMGGLDIRYLPNITVCKLDTTTTPPVP
                     TSNCKTTTDLGQGAIDGKFDHVGKIKGPILRGLSARAPYFHNGSARTLLDAVHFYESR
                     FGLVLTHEEESDLVAFLSSL"
     gene            3339..>4386
                     /locus_tag="VII37_07225"
     CDS             3339..>4386
                     /locus_tag="VII37_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5069561.1"
                     /translation="MTPRYLLWKRCVLICALLIPVAVFGNSGSIIASNRSDKSANKTP
                     PAATQEIPVLPLDNPARRNMIWLTSPVTDARQYLIQQVEQKFASGEQNYKAGHLEAAR
                     KDFDDAVDWMLQSGYDPNSDPQLSELFHRVVDTVYTYELQAFRAGDGFNEAPAVPAPI
                     DEVAEMTFPVDPRLKARAEEAAKNISHDLPLTVNDEVLSFLNFFQTPRGRAIVETGLR
                     RAGRYRGMIARVLSEEGVPQDLIYLAQAESAFQPTALSRAGARGMWQFVAYRGQEYGL
                     RHTWWLDERQDPEKATHAAAQHLRDLYHMFGDWYLAMAAYNCGPGNVQKAIERTGYAD
                     FWELYRRNVLPRETK"
BASE COUNT          934 a         1277 c         1231 g          944 t
ORIGIN      
        1 gatcggtagc gatgattaac gatagcgggt cgagctggcg ggcacgctcg ctttcctcca
       61 aggcttccga aaaacgtccc tgaaaggaaa ggcactctgc ataccattgg tgggcggtcg
      121 cgtattgggg gtcgagctgg atggcgcgac ggtactcttt ctccgctgtt tgccagtcgt
      181 aatcgtagtt ttcagcaatc agtgccagcg aggtatgcgc ctcagccaga ctgacatcaa
      241 gctgcatggc tcgcaatgcg gccgcgcgtg ctttagggat ggcttccgtt tgcggtacga
      301 aggaccaact gctcatgagt gcatagctat ccgccagccc ggcataggct cgcgcagaat
      361 taggatcgct ggcgatggac tgctgaaaat aatccgcggc ttgctgaaac ccgtccttgg
      421 tccgtttgtt ccagaagtat cggcctttaa gataaagatc gtaggtctca tatgaagtaa
      481 gagaagccag cttgcggtct gcggcccgca gcttgtgacc ctggcccagc cttagctgta
      541 tttcgtcagc cgtttcttgg gcaatttctc cctggagagc taaaagactt ttgagctctc
      601 gatcatattg ccgcgaccag atgtgcgtct ggtctttcat ctgcaccaac tgagcggtga
      661 tgcgcacgcg gtccgcttcg cggcgaaggc tgccctccag cacatattgc acacctagct
      721 cacgcccgat ctgcccggct gcctcggtgg tgtgtttgta gtgcatcacc gaggtccggg
      781 caatcactcc cagatgttct ggatccaggc gcccaagctg cgcgatcatc tcttcggtga
      841 ggccgtcgct taggtattcc tgcgcagggt ccccggtcag gttttcgaat ggaaggacgg
      901 ccagcatcaa acgctcggcg gacggctgcg gcctggcttg cattcgcgac cacgtaaaat
      961 agctgaccgt gagtgtgagt agaatcgcgc atatacccaa aattaaagac ccacggcgtg
     1021 cgggcggccg tacgacttta ggggcactcg gtggaggttc gagcacgctc gcgcgagggg
     1081 tttctgcgag cgaggggacc tgttctacag gagcgataaa tcgatacccc aaacgaggaa
     1141 ccgtttcgat gtatcgtgaa ctctcctggg aatcgcccag tacggcacgc aactttctga
     1201 tcgcgctatt tagactccgc tcataatcca caaaagtttc ggaatcccac aacttgtctc
     1261 gcatttcctc gcgggacacg acttggccgg gtctctcaag gaggatcgac agcacgcgaa
     1321 acggctgacc cggcagccga atcctcaccc catgtttgcg cagttcacgt gcagccagat
     1381 tgacctggaa gacgccgaag cgcaaaacgc gggtgggcga caattctgtt ctttctgaca
     1441 tgttttactg cgccccagcg caatcgtctc gcgaagggcg agttacctgg cgacctaacc
     1501 caaagacaat ctaggacttg gctggtcaca aatagatgat ggcagctgca ttgtgtttgt
     1561 atcactaacg gcatagcttc acggcaactt ttttgtactt atcctgaggc aatcataaat
     1621 ctattggcgc ctagcggcgc ccaaagggtg tgtttccatg cgcactatct cggcccggcg
     1681 atcaggtttt agccgccgct tgtatccatt cgcctcgtta agctctcaat ccaaacgcgc
     1741 cttcgttagt gtaactatcc tgacggtcgc gatgctgatg gccatgagtg ccatcagcag
     1801 gcaaccgctc aacttcattc ctaacggcac acctttcagg gattcccggg gcgcctcgga
     1861 aacctacagc accgttggcg gcggcatcga tcaaaccggg ccttttttcc aaagtatggg
     1921 caccaacggc cgttcctgcg ccacttgcca ccagcccagt gacggtatgt ccattgccgc
     1981 cgaagaagtc caggaacggt tcttcatgac gcgcggccgc gacccaattt tccgcaccgt
     2041 cgacggctcc aattgcaatc acaacatcga cgtgtcgacg ttagagggca tgtatgacgc
     2101 gtacagcctg ttgcgaacgc gcggtttgat ccgcatacct atcgccatcc cggcaaccgc
     2161 tgactaccag gtggtcagcg tgcaaaacca gtacggctgt aacgaatctg atgtggtttc
     2221 catgtaccgc cgtccgttac ccaccaccaa cctgcgattc ctaagcgcgg tgatgtttga
     2281 tggccgcgaa tcgactccat tgaccggtac catgaaaatc gctgacgcaa actatccaac
     2341 ttctctcgta agtaatttga tccatcagtc tgtggatgcc actaccattc acgcgcaggg
     2401 ggacggaacc aggccgacgc cggctgagca gcaggaaatt gtgaattttg aaatggcgct
     2461 attcaccgcg caaaccttcg gcgacgatat cggaccgtta aacacccacg gcgcgaatgg
     2521 cgggccgcag ccgctgatta cacagccatt ctttatcagt atcaactcaa gcgttcatcc
     2581 gatagttcag accctggagc tgccggctgg acttgtgacg cgaggcgata atatcttcac
     2641 gccggcgatt tttgatacgt ttggcgcgtg gtcggagttt ccggagcatt cgcgccgggc
     2701 tgcggtagcg cgcggtgagg cgattttcaa ttccagggcg attaatatta ccggtgtggc
     2761 cggaatcaac gaccccgcag gggcgggcgg cctgcttcca aatggggttc aggcatttca
     2821 aggaacgtgc gggacgtgcc acgatactcc caatgtcggc aatcactcct tcccgacacc
     2881 gttgaacatc ggcaccggtg atccgagtcc caccaatcct tccgccaaca tgggcgggct
     2941 ggatatccgc tacttgccga acatcacggt ctgcaagctg gacactacca ccactccacc
     3001 tgtgccgacg agcaactgca agaccaccac cgatcttggc cagggggcca tcgatggaaa
     3061 attcgatcac gtgggcaaaa tcaagggacc tattcttcgt ggtctctcag cacgcgcacc
     3121 gtactttcat aatggttccg ccaggactct gctggatgcg gtgcattttt atgaaagccg
     3181 ctttggtctg gtgctgacgc acgaggaaga atcggacctg gtggcattcc taagttccct
     3241 gtagcgctcc gcgccgcaaa gtcgatgtgg tgtgaacgcc aataccttca cggtgcaaag
     3301 cgttgaccgc tgaggcatgc ggtccttacc atggatgaat gacgccgcgg tatctgctct
     3361 ggaaacggtg tgtgttgatt tgcgcgctgc tgatcccagt ggccgtgttt ggtaattctg
     3421 gatcaatcat agcgtcgaac cgctccgata aatctgccaa caaaacgcca ccagcggcca
     3481 cgcaggaaat tcccgtgttg ccgctcgaca atccggcgcg gcgcaacatg atttggctca
     3541 cctcgccggt cacggacgcg cggcaatatt taattcagca ggtagaacaa aaatttgcgt
     3601 ccggggagca aaactacaaa gccgggcacc tggaagccgc gcgcaaggac ttcgacgacg
     3661 cggtcgattg gatgctgcaa agcggctacg accccaactc cgacccgcaa ttgagcgaac
     3721 tgttccaccg cgtggtggac actgtttata cctacgagct gcaggccttt cgcgccggcg
     3781 atggtttcaa tgaggcgccc gcggtgccgg caccgattga tgaagttgca gagatgacct
     3841 ttcccgtgga cccgcggctg aaagcgcgcg ccgaggaagc ggccaaaaat atttcgcacg
     3901 atttacctct gaccgtaaac gatgaagtgc tgtccttcct gaatttcttt cagaccccac
     3961 gcgggagagc catcgtcgaa actgggctgc gccgcgccgg ccgttaccgc ggcatgatcg
     4021 cgcgagtgct cagcgaggag ggcgtgccgc aggacctcat ttacctggcg caggcggaga
     4081 gtgcgttcca gcccaccgcg ctttcgcgcg cgggagcccg tggcatgtgg caattcgtcg
     4141 cttaccgtgg acaggagtat ggcctgcgcc acacctggtg gcttgacgag cgccaggatc
     4201 cggagaaagc tacacacgcg gccgcgcagc atttgcgcga tttgtatcac atgtttggag
     4261 actggtattt agccatggcc gcctacaact gcgggccggg caacgtgcaa aaggccatcg
     4321 agcgcaccgg ttacgcggac ttctgggagt tgtaccgccg caacgtgctg ccgcgcgaaa
     4381 cgaaaa
//
LOCUS       DATCBB010000654         1493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_377651, whole genome shotgun sequence.
ACCESSION   DATCBB010000654 DATCBB010000000
VERSION     DATCBB010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377651"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VII37_07230"
     CDS             complement(<1..329)
                     /locus_tag="VII37_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069562.1"
                     /translation="MTNFSKSICKIALHVCATIIAIGAIDISVRAQSQPDPATPPAVN
                     QLAHDIFKQLIEINTTDSVGNVTTAAEAMAKRLRDAGFTDKDVIVAGPNEKKKNLVVR
                     FHGTVRR"
     gene            complement(342..1454)
                     /locus_tag="VII37_07235"
     CDS             complement(342..1454)
                     /locus_tag="VII37_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY5069563.1"
                     /translation="MGTLEVAAPGLILPQEPPDLLDLSLVLPTYNESAHIDAIVDDDS
                     PDCTWEKALQLSQKFPQVRAVRRQDQRDLSTAVLRGWQIARGDVFAVMDADLQHPPET
                     IPELWRAIRRGADLAIASRNIEGGGVSDWTLPRRIVSRCAQLLGLVVLPEVTGKVSDP
                     MSGYFMVRRSALEGRELHPLGYKILVEVLARGTVEKISEVPYVFRETPHRPSKHSAAV
                     SAQYLQHLIRIRIHLLKTSTFLRFCIVGASGTLIDTAMLFLLSDPRTLNWNLTGSKII
                     AAELALTNNFIWNDLWTFGKFSNRQNTFRQRARRFLKFNLLCSVGIILNLIILNIGFK
                     VFHMNRYIANLSAIFIVTFWNYNTNRKLSWRTTAKE"
BASE COUNT          324 a          326 c          485 g          358 t
ORIGIN      
        1 ttacgcctga cggtgccgtg aaagcggacg acgaggtttt ttttcttctc gttgggaccg
       61 gcgacgatga cgtctttgtc ggtgaatcca gcgtcgcgta ggcgtttggc catggcctcg
      121 gcggcagtgg ttacgttgcc cacggagtcg gtggtgttga tttcgatcag ttgcttgaag
      181 atgtcgtggg caagttggtt gacggcgggc ggcgtggcgg ggtcaggctg cgattgagcc
      241 cgaacggata tgtcgattgc gccgatggcg attatggtcg cgcacacgtg aagtgcgatc
      301 ttgcaaatac ttttggaaaa atttgtcatg ggaatgattt tctattcttt tgcggtggtg
      361 cgccagctaa gtttgcgatt ggtattgtag ttccagaaag tgacgatgaa aattgcagac
      421 aaattggcga tgtagcggtt catatggaat accttgaaac cgatattcaa gataatgaga
      481 tttaaaataa ttcctaccga acatagcagg ttgaatttca ggaagcggcg agcgcgttga
      541 cgaaaagtat tttggcggtt ggagaatttg ccgaaggtcc acaaatcgtt ccagataaaa
      601 ttatttgtga gtgccagttc ggcggcaatt attttgcttc cggtgagatt ccagttgagg
      661 gtgcggggat cgcttaagag aaagagcatg gcggtgtcga tcaaggtgcc gctggcgcca
      721 acgatgcaaa agcggaggaa ggtggaagtt ttcagcaggt gaatgcggat gcggatcagg
      781 tgctgtagat actgggcgga aaccgcggcc gaatgtttgc tggggcgatg aggagtttcg
      841 cgaaatacgt aaggtacttc tgagattttc tccaccgtgc cgcgcgccag cacttcgacg
      901 aggattttgt atcccagggg atgcaactcg cggccctcca atgcggaacg ccgcaccatg
      961 aaatagccgc tcatggggtc gcttactttc ccggtgactt cagggagcac gacgagtccc
     1021 agcaattggg cacagcgcga aacgatacgg cgcgggaggg tccagtcgct gacgccgccg
     1081 ccttcgatgt ttctgctggc gatggccagg tcggcgccac gcctgatggc acgccacaat
     1141 tcgggaattg tttcgggggg atgttgcagg tcggcatcca ttacggcaaa aacgtcgccg
     1201 cgggcgattt gccatccgcg caaaacagcg gtggacaggt cgcgctggtc ctggcggcgt
     1261 acggcgcgga cttgcggaaa tttttgggag agttgaagcg ctttttccca agtgcaatcg
     1321 ggactgtcgt cgtcgacgat ggcgtcgatg tgggcgcttt cgttgtaagt ggggagaacc
     1381 agggagagat ccaaaagatc tggaggctcc tgcggaagga tcaggccagg ggcggctact
     1441 tccagagtac ccatctgcag ttattgttct ccaagatttg gggttgaaat aat
//
LOCUS       DATCBB010000655         1766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_476898, whole genome shotgun sequence.
ACCESSION   DATCBB010000655 DATCBB010000000
VERSION     DATCBB010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476898"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VII37_07240"
     CDS             complement(<1..463)
                     /locus_tag="VII37_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5069564.1"
                     /translation="MTTTYHPSVHRFAIFVVCWTILLLIAGALVTSSEAALSVPDWPL
                     SYGTLTPPMVGGIRYEHSHRVIAGVLGLLSIVLAVWVWAKDERRWLRWFSVIAVAGIA
                     AQAVLGGEVVRQLLHYWLPVAHACFAQIVFASILSIAVFTSRWWVSDQPQVD"
     gene            complement(595..1662)
                     /gene="hemH"
                     /locus_tag="VII37_07245"
     CDS             complement(595..1662)
                     /gene="hemH"
                     /locus_tag="VII37_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY5069565.1"
                     /translation="MAAKKRVGIVMFQLGGPDSLNAVEPFLLNLFLDPDIIPLGPLGL
                     LRKPIAKLISSRRSKPVMGRYAQIGRRSPIATLTERQRVRLVNSLRPHIDPIAVIAMR
                     YWHPMSIEALDTLRNAGPLDEIVLLPLYPHYSYATTLSSMKEWHRVADRAKWTTSRGA
                     PPERVVESFYNHPLYIRGLVENIGRVLRQFPDSSRIHLLFSAHGLPMSLVEKGDPYPH
                     QIESTVRLTCERGAQQFPNWPRKHLLCYQSRVGPAKWLQPPLTTTIERLAHEGVKEML
                     VVPISFVTEHIETLHEINIEAREEAKKLGVERFRMMPALGDSPYFIAALKDLVLRAVG
                     LESVNPAIPPSPGIPATAAQA"
BASE COUNT          403 a          476 c          542 g          345 t
ORIGIN      
        1 catccacttg gggctgatcg gatacccacc acctgctcgt aaagaccgcg atgcttaaga
       61 tcgacgcaaa gacgatctgc gcgaaacaag catgcgcaac cgggagccag taatgtaaaa
      121 gctgacgcac cacttccccg ccaagcaccg cctgcgcagc tatgccggcg accgcgatca
      181 cgctaaacca tcgcagccag cgtcgctcgt ctttggccca cacccaaaca gccagaacga
      241 tggacagcag ccccaaaact ccggcaatga cgcgatgaga gtgttcgtag cggatgccac
      301 ccaccatcgg cggcgtgagc gtgccgtagg aaagcggcca atccggaacg gagagcgcgg
      361 cctcgctgga agtaaccaga gcgcctgcta taagaaggag gatggtccag cagacgacaa
      421 aaatagcaaa ccgatggaca gacggatggt aagtggtcgt cattggtccc agtcccaacg
      481 gcggattata catgagtggt ggatgcgcgc taacgggaaa atagcttcaa attcttcaaa
      541 gatttagaac taatcgaggc gcggagttcg gaccgcggtt ccgcgccagg cggttcaagc
      601 ttgcgcggcg gttgcgggaa taccgggtga gggaggaatt gccggattca cggattcgag
      661 cccaacggct cgcagcacca ggtcttttag agcggcgatg aagtatggcg agtcgcccaa
      721 ggctggcatc atgcgaaacc tttcgacccc gagtttcttc gcttcctcgc gagcctcgat
      781 gttgatttcg tgaagtgtct caatgtgttc ggtgacgaaa gaaatgggca ccaccagcat
      841 ttcctttacg ccttcgtggg cgagacgctc gattgtggtg gtcagcggtg gctgcagcca
      901 tttggcgggg ccaacgcggc tttgatagca cagcaggtgc tttcggggcc agttgggaaa
      961 ttgttgcgcg cctcgttcgc acgtcaagcg taccgtggac tcgatctgat gcgggtaggg
     1021 atcacccttt tcaaccaggc tcatgggtag tccgtgagcg ctgaagagca gatgaatccg
     1081 cgagctatcg ggaaattgcc gcagcacgcg gccgatgttt tccaccaggc cgcggatgta
     1141 cagcggatga ttatagaaac tttctactac gcgttcgggc ggcgctccgc gggacgttgt
     1201 ccacttcgca cggtccgcga cgcggtgcca ttccttcatg ctgctaagcg tagtcgcata
     1261 ggaataatgc ggataaagag gcagcaagac aatttcatcg agtggcccag cgttgcgcag
     1321 ggtgtccagg gcctcgatgc tcatgggatg ccagtaacgc atggcgatga ccgctatcgg
     1381 atcgatgtgt gggcgaagtg aattcaccaa gcgaacgcgc tggcgttcgg tgagcgtagc
     1441 aattggagaa cgacggccaa tttgcgcgta acgtcccatg accggtttgc tgcgacgcga
     1501 agaaattagt ttggcgatgg gtttgcgcag caagcccaga gggccgagcg ggatgatgtc
     1561 cggatcgagg aaaagattca ggagaaacgg ttcgaccgca ttgagcgaat ccgggccgcc
     1621 gagttgaaac atcactatgc ctacgcgctt ctttgcggcc acttatttgc cccggtgcaa
     1681 tgagtttttc aggcgctcaa tgtcttcgtg cagcgccgcg gcgcggcgcg ctaccgtaag
     1741 gtagtaggca aagaaaattc cccacg
//
LOCUS       DATCBB010000656         1197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_85739, whole genome shotgun sequence.
ACCESSION   DATCBB010000656 DATCBB010000000
VERSION     DATCBB010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85739"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..>1197
                     /locus_tag="VII37_07250"
     CDS             147..>1197
                     /locus_tag="VII37_07250"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5069566.1"
                     /translation="MNDALMPPAFTRTLRLKVKREAYPWLNKAAFETNQVFNYCNEAS
                     LLAATRTDLKRKWLTGFDLCNLTSGATKHFEHIGADTVQSICVHFAQKRIAAKKLKLR
                     WRASGGSKRALGWVPFKAASVKRKGNSLRFAGKNFRVFERAYLGEHKFRDGCFAQDAL
                     GDWYLCIPVRMTIEQTVAPREAVGIDLGLKTIATTSDGEKLEAGRWTHGYAVALANAQ
                     RRGHKKQAKRIHRKAARCRADALHKFSRQIVDQYQNVIVGDVSSTKLVKTKMAKSVLD
                     SGWGMLKTQLQYKGRQAGRSVQVVSEKYTSVACSSCGCLSGPRGVNGLIVRSWICCEC
                     GSSHDRDVNAAKNILA"
BASE COUNT          284 a          303 c          374 g          236 t
ORIGIN      
        1 cgccatctta ccgatccact gttgaacgac cgataaataa cccggtcggt gatagatttt
       61 tggctatact catttttgcg caacgaagca acagggacgt tgcgcccccg ttgcgacagt
      121 tgcgttagca gaggagctaa ggaaaagtga atgatgcgct catgccgccc gcgttcacgc
      181 gcacgcttcg tcttaaagtg aaacgtgaag cctacccatg gttgaacaag gcagcgttcg
      241 agaccaatca ggtgttcaac tattgcaacg aggcgagcct gctggctgct acgcgcaccg
      301 atcttaagcg caagtggttg acggggtttg atctttgcaa tctcaccagc ggcgcgacga
      361 agcatttcga gcacatcggg gcggacacgg ttcagtcgat ctgcgtacac tttgcgcaga
      421 agcgcattgc ggcgaagaag ttaaaactgc gctggcgcgc aagtggcggc tcgaagcggg
      481 cgctgggctg ggttccgttc aaagccgcga gcgtgaagcg caagggaaac tcactgcgtt
      541 tcgcaggaaa gaacttccgc gtatttgagc gcgcgtacct gggagagcac aagttccggg
      601 acggctgctt cgcgcaagat gcgttgggag attggtatct gtgcattcct gtgcgcatga
      661 cgatcgagca gacagttgcg ccgcgtgaag ccgtaggtat tgatctcggc ctcaagacta
      721 tcgcgacgac gagcgacggc gagaagctag aagcgggacg atggacgcac ggctacgcgg
      781 tagcgcttgc caatgcgcag cgccgcgggc acaagaagca agcgaagcgc attcatcgca
      841 aggccgcgcg ttgtcgcgcc gacgccttgc ataagttttc aagacagata gttgatcagt
      901 atcagaacgt aatcgtaggc gacgtgagca gcaccaagct agttaaaacc aagatggcaa
      961 agagcgtttt ggatagcggt tggggcatgc tcaaaacgca gctgcaatat aagggccggc
     1021 aggcgggcag aagcgttcaa gtcgtcagcg agaagtacac cagcgtcgcc tgttcttcgt
     1081 gcggatgctt aagcggtccg cgcggggtca acgggctgat tgtaaggtca tggatatgtt
     1141 gcgagtgtgg tagctcgcat gatcgcgacg taaacgccgc gaaaaatatc ctcgccg
//
LOCUS       DATCBB010000657         1140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_476956, whole genome shotgun sequence.
ACCESSION   DATCBB010000657 DATCBB010000000
VERSION     DATCBB010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476956"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..>1140)
                     /locus_tag="VII37_07255"
     CDS             complement(173..>1140)
                     /locus_tag="VII37_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5069567.1"
                     /translation="VYVRLASWTKSSLIFHYDPKKNVLEDTKIIPPSPVDFSGIESEE
                     VKAKSQDGTMVPLSIVHQRGLKMDGSHPTLLHGYGSYGITYDPGFDPTSLAWLERGGV
                     VAVAHVRGGGEYGEDWHSGGRKKTKENTITDFLACAQYLIDHKYTSPQRLAGEGTSAG
                     GITIGGAITERPDLFGAALDNVGMSDDLRAELQVNGPANIPEFGTVKNADDFKNLLAI
                     SAFHRIKSHTAYPAVLLTTGINDPRVDPWQMNKMTARLQAANSSGKPVLLRVDYDAGH
                     GGIGATKSQHTALLTDQYSFLLWRLGDPEFAAPNGGAKESVAKQP"
BASE COUNT          243 a          356 c          266 g          275 t
ORIGIN      
        1 cgccggcggc cggttcaagc cctctatctt cggctggatg gtgcacgtgt acccctacga
       61 agacggcctg gacaaaatct tcgccatgca tcaccacatg gattagtttt ttagtaagcc
      121 ggacttaact cgcgattgcg agcagcagac ctgaaaccat catttcggcg ctctacggtt
      181 gctttgcaac tgattccttc gccccgccgt tgggcgcagc aaattccgga tcgcccagtc
      241 gccacaacaa aaatgaatat tgatccgtca gcaacgcggt gtgctgcgat tttgtcgctc
      301 caattccgcc atgcccagcg tcataatcca cccgcagcag caccggtttc ccgctggagt
      361 tcgccgcctg cagtcgcgcc gtcatcttat tcatctgcca cggatcaacg cgcggatcat
      421 taattcccgt ggtcaacaac accgcgggat acgcggtgtg actctttatc cgatgaaacg
      481 cactgatcgc cagaaggttt ttgaaatcat ccgcgttctt taccgtcccg aattccggaa
      541 tattcgcggg gccattcact tgcaactctg cccgcaaatc gtctgacata cccacattat
      601 cgagtgccgc cccaaataaa tcaggccgct cggtaattgc gccgccaatg gtgatcccgc
      661 ctgcgctggt gccttcgccc gcgaggcgtt gtggcgaagt gtatttgtgg tcgatcagat
      721 attgcgcgca cgccagaaaa tccgtgatgg tattttcctt cgttttctta cgtccgccgc
      781 tatgccaatc ctcgccatat tctccgccgc cgcgaacgtg cgccacggcc accactccgc
      841 cgcgttctaa ccacgccagc gaggtaggat caaatcccgg gtcatacgtt attccgtaag
      901 atccgtaccc gtgcagcagc gtcggatgcg atccatccat tttcaacccg cgctggtgca
      961 cgatcgaaag cggcaccatc gtgccatctt gcgatttggc cttcacttct tccgattcaa
     1021 ttcccgaaaa atccaccggc gaaggtggaa ttatcttggt atcctccagc acattttttt
     1081 taggatcata gtgaaaaatc agtgacgatt tggtccacga agccagcctc acataaactc
//
LOCUS       DATCBB010000658         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_280636, whole genome shotgun sequence.
ACCESSION   DATCBB010000658 DATCBB010000000
VERSION     DATCBB010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280636"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="rpsB"
                     /locus_tag="VII37_07260"
     CDS             complement(<1..440)
                     /gene="rpsB"
                     /locus_tag="VII37_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY5069568.1"
                     /translation="MAVEITMKELLEAGVHFGHQTRRWNPKMKEYIFGERNGIHIIDL
                     QKTLKMFREASRFVGELSAQGRNVLFVGTKRQAQEAVAEEAKRCGAFFVNHRWLGGTL
                     TNWSTLQKSIKRLKLLKAMSEDGRMNELSKKEKARLEREMKHLF"
     gene            complement(656..1051)
                     /gene="rpsI"
                     /locus_tag="VII37_07265"
     CDS             complement(656..1051)
                     /gene="rpsI"
                     /locus_tag="VII37_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY5069569.1"
                     /translation="MGKQTWILGTGRRKESVARVFLRAGSGKFKVNGRTAEEYFPNVA
                     WKHNATEPLKFTSLHDQVDVVITAKGGGVGGQAGAVRMGLARAISVFNPELRPALRKN
                     GFLTRDSRMKERKKYGQKGARKRFQFTKR"
     gene            complement(1248..1709)
                     /gene="rplM"
                     /locus_tag="VII37_07270"
     CDS             complement(1248..1709)
                     /gene="rplM"
                     /locus_tag="VII37_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY5069570.1"
                     /translation="MTVSMRTYVAKGEEAEALKVGANWFVIDATDMVLGRLATKVARI
                     LIGKDNPSFTPYLDSGDHVVVINADKIRMTGNKVEQKVYYSHSGYPGGLKEVPAKRLR
                     VAKADWMVREAVLGMLPKNKLRARRAKKLRIYRDAAGLARHAGQKPQAMAS"
     gene            1865..>2052
                     /locus_tag="VII37_07275"
     CDS             1865..>2052
                     /locus_tag="VII37_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069571.1"
                     /translation="MEVVAGVDLGGTAVNYTLVNREEKFLIEGLCEHPSLAKQGPDVC
                     LKQIEDGLQIAADRAGVQ"
BASE COUNT          458 a          608 c          513 g          473 t
ORIGIN      
        1 tgaaagaggt gcttcatttc gcgctcgagc cgcgcttttt ctttcttgga gagctcgttc
       61 atgcggccat cttcgctcat ggccttcagt aacttcaaac gcttgatgga tttctgaagc
      121 gtggaccaat tcgtcagcgt gccacccagc cagcggtggt tcacgaaaaa tgcgccgcag
      181 cgcttcgctt cttcggcgac cgcttcctgg gcctggcgct tggtgcccac aaaaagaacg
      241 ttcctgcctt gcgcggaaag ttcgccaacg aagcggctcg cttcgcggaa cattttcagc
      301 gtcttttgaa gatcaatgat gtgaatgcca ttgcgttcgc cgaaaatata ctccttcatt
      361 ttcggattcc agcgtcgtgt ctggtgaccg aagtgaactc cagcctccag caactccttc
      421 attgtaattt ctactgccaa gtttcctcct ttaaggattg ctgtttgcgc tcacgagcga
      481 aaatgctctc gcgaaagaac agcctttttg ccggctccat gccggccgtg atctcggcgg
      541 gccgcttacg cgaaaccgcc ggagctcgaa gctgagcagc cactaaaggc atgcccagca
      601 aaatccaaaa caactcgcga attcctgcgg tccaacaccg cgggatccgg gtcgcttagc
      661 gcttggtgaa ctggaagcgc ttgcgtgcgc ccttctggcc gtacttctta cgttccttca
      721 tgcgcgaatc gcgagtcaga aatccatttt tgcgcaacgc cggccgcaat tccggattaa
      781 acacggaaat ggcgcgtgcc aggcccatgc ggaccgctcc agcctgcccg ccgactccac
      841 cgcccttggc ggtgatgacc acgtccacct gatcgtgcaa gctcgtgaat ttcagcggct
      901 ccgtggcatt atgcttccag gccacgtttg gaaaatattc ctccgccgtg cggccattga
      961 ctttgaactt gccagaaccc gcgcgcagaa aaactcgcgc aacagattcc ttgcggcgac
     1021 ctgtacccag aatccaagtt tgcttgccca tgaggggcga accagtagaa ctcaacgttc
     1081 ctcctaaact ccaaaacaaa agctcgttcg cgaaatcgat caaagcgatt tcgcagacac
     1141 ttagtgcgtg cgtctgaaaa atcccgatgg aaaaccctgg agactggaat actgcaggtt
     1201 gttacaccca ccagaacaac aatccccgaa ccttacggtt ctacgaacta acttgccatc
     1261 gcctgcggct tctgccccgc gtgacgcgct aaaccagcgg catcccggta tatgcgcaat
     1321 ttcttggcgc gtcgcgcacg caatttattt ttcggcagca tcccgagcac tgcttcgcgc
     1381 accatccagt cggccttagc cacgcgcaaa cgcttcgctg gcacttcctt cagcccgccg
     1441 ggataaccgc tgtgcgagta ataaaccttt tgttcgacct tgtttccggt catgcggatc
     1501 ttgtccgcat tgatgaccac cacgtgatcg cccgaatcga gatacggagt aaaactcgga
     1561 ttatctttgc cgatcaaaat gcgagccact ttggtggcca gccgccccag aaccatatcc
     1621 gtcgcatcaa tcacaaacca gttagcgcct actttgaggg cttcggcttc ttcacccttg
     1681 gctacgtacg tcctcattga aacggtcaca cccttccctg tagagacaaa cataaatctt
     1741 agagtacacg ccggttaatg tcaaaggaat gtattgtggt agcgagtgaa gcccgttggc
     1801 caagaacggt gtagactctc agacattttc cgggcgccca ccgcgtctga aaggatacta
     1861 aaacgtggaa gtcgtggctg gagtggatct gggcggaacc gccgttaact acacgctcgt
     1921 taaccgtgaa gaaaaatttc taattgaggg cttgtgcgag cacccttcgc ttgcaaagca
     1981 ggggccggac gtgtgtctga agcagatcga agatggtttg cagatcgccg ctgaccgggc
     2041 tggcgtgcag at
//
LOCUS       DATCBB010000659         1438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_152056, whole genome shotgun sequence.
ACCESSION   DATCBB010000659 DATCBB010000000
VERSION     DATCBB010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152056"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VII37_07280"
     CDS             <1..1309
                     /locus_tag="VII37_07280"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEY5069572.1"
                     /translation="LPLVYTENPITKKHNLGIYRMQVYDGQSAGLHWQIQKGGGFHYH
                     AAEEMNQPLPVTVFLGGPPALIISAIAPLPEDVPELILASLLGGEKFRMAKNPAGAHR
                     LVAESEFALIGHAQPHERRAEGPFGDHYGYYSLRHEYPVFHCEAIAHRRDAIYPATVV
                     GKPRQEDFFIGDYLQSLLSPLFPLVMPSVRNLWSYGETGFHSLAAAVVRERYPREALV
                     AGFRILGEGQLALTKFLILTDTSVDLHDFPRVLEHVLARVRWETDFFIFANTSMDTLD
                     YTSGRVNEGSKAILMGLGDAIRDLPKEFRDELPRGITRAESFCGGCLVAEGAPYLDEP
                     DQAARLAREKVFAEWPLVILHDDAGVARSAIDFLWSTWTRFEPAGDIYAAETNVVRHH
                     LAYRGPMVIDARKKPSLPEELIVRDDIAKLVDLRWREYFPNEL"
BASE COUNT          283 a          384 c          440 g          331 t
ORIGIN      
        1 gctaccgctg gtctataccg aaaatccaat cacgaagaaa cacaacctgg gcatttatcg
       61 catgcaggtt tacgacggac agtccgccgg cctgcattgg cagatccaga aaggcggagg
      121 ctttcactat cacgcagcgg aagagatgaa ccagcctctg ccggtgacgg ttttcctggg
      181 cgggccaccg gcgctgatca tttcggcgat cgcgccgttg cctgaagatg tgcctgaatt
      241 aatccttgcg tctctcctcg gcggagagaa attcagaatg gcgaagaacc ctgcgggcgc
      301 gcatcggctc gttgcagaaa gtgaatttgc acttattggc catgcgcaac cgcatgagcg
      361 ccgagcagaa ggaccgttcg gtgatcatta tggctattac tcgctgcggc atgagtatcc
      421 ggtttttcac tgcgaggcga tcgctcatcg gcgcgacgcg atttatcctg cgacagttgt
      481 aggcaagcca cgccaggaag attttttcat cggcgattat ctacagtcgc ttttgtcgcc
      541 gttgtttccg ctggtgatgc cgagcgtgcg taatttgtgg tcgtatggcg agactggatt
      601 tcattctctc gccgcggccg tcgtgcgcga acgctatccg cgcgaggcgc tggtggccgg
      661 cttccgaatc ctcggcgaag ggcaactggc gctgacgaag tttctgattc tcactgacac
      721 ttccgtagac ctgcatgatt ttccgcgcgt gctggagcac gtgctggcgc gcgtgcgttg
      781 ggagacggac ttcttcattt ttgcgaatac gtcgatggac actttggatt acaccagcgg
      841 gagagtgaat gaaggcagta aggcaatcct gatgggtctc ggtgacgcga ttcgcgattt
      901 gccaaaggag tttcgcgacg aactcccgcg cggcatcacc cgggccgaaa gtttctgtgg
      961 cggctgtctg gtagcggaag gcgcgccgta tttggacgag ccggaccagg cagcgcgtct
     1021 ggcgcgcgaa aaagtattcg ctgaatggcc tctggtgatc ctgcatgatg atgccggcgt
     1081 cgcacgctca gctatagact ttttatggtc aacctggacg cgcttcgaac cggccggaga
     1141 tatctatgcc gccgagacaa atgtggttcg tcatcatctc gcttatcgcg gcccgatggt
     1201 gatcgacgcg cgcaagaagc ccagtctgcc ggaggaatta atcgtgcgcg atgacatagc
     1261 caagttggtc gatttgcggt ggcgtgaata ttttccgaat gagctgtagg aagtttggaa
     1321 gtggggtgac tagtgctggg agcgcggacg tcccgtccgc acagcgccgc aggcgcttag
     1381 tttcttgaaa tgctcttcgc gcttcgccct caatgcggac gagacgtccg cgctccca
//
LOCUS       DATCBB010000660         4126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_280657, whole genome shotgun sequence.
ACCESSION   DATCBB010000660 DATCBB010000000
VERSION     DATCBB010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280657"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VII37_07285"
     CDS             complement(<1..195)
                     /locus_tag="VII37_07285"
                     /inference="COORDINATES: protein motif:HMM:NF044766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069573.1"
                     /translation="MSINQDKLHEFLGRAIVDFGATFNAALIRIGDKLGLYKALAKGG
                     PQTPAQLASATNTSERYNREW"
     gene            complement(281..529)
                     /locus_tag="VII37_07290"
     CDS             complement(281..529)
                     /locus_tag="VII37_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CstA-like transporter-associated
                     (seleno)protein"
                     /protein_id="HEY5069574.1"
                     /translation="MQSAQTMTAARMQNLRGLIVALRLKRLTSKLWQGIREWCGDNAY
                     ERYIQAQRTKPEKSCLLTPAEFYVERVNRRYSRPNRCC"
     gene            complement(532..2652)
                     /locus_tag="VII37_07295"
     CDS             complement(532..2652)
                     /locus_tag="VII37_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY5069575.1"
                     /translation="MKRPLTVVIWIVVALIGAAALGTIAFHRGEPLNATWFVVAAGCS
                     YLVAYRFYSAFIAARLLALDDTRATPSERHDDGRDFVPTNKWILFGHHFAAIAGPGPL
                     VGPTLAAQFGYLPGTLWLIAGAVFAGCVQDFIILFCSIRRDGKTLGEIAREEVSQRGG
                     FIAQLAVLAIMVILLGVVALVIVNALKSSPWATFTLAATVPIALLLGLYLRFLRPGRV
                     LEASFIGVALVLFVVVAGQWVADSPSWAHVFTLSGVTLAFALIAYGFAASALPVWLLL
                     APRDYLSAFIKAGAIFSLAAGILLVRPQVLMPPLTRFIDGSGPVFAGKIFPFCFITIA
                     CGAISGFHSLISSGTTPKMIIRESHARFIGYGAMLMESFVGVMAMVAACAMTPGVYFA
                     INSPASIVGATSDAAAATISSWGYPLQAQTMTDLARAVGEQTLLNRAGGAPSLAVGMA
                     QIFSSTIGGPRLLSIWYHFAIMFEALFILTVLDAGTRVGRFMVQELGGRVWKPFARTG
                     WWPGILLSSALIVGAWGYFLYQGVVDPLGGINSLWPLFGISNQLLAAVALVVATTILL
                     KMKRIRWIWLTLIPMTWLVIITMTASYQKIFDPNPRLGFLSYAKALATQIAAGKIPAA
                     KIADTQRVIFNQRLDAAVTAVLAAMIIVLIVEALMQWYRILSKNPKSVLHETAYVATR
                     WPENFSGAAYAATAAEPETPAMEG"
     gene            2773..3429
                     /locus_tag="VII37_07300"
     CDS             2773..3429
                     /locus_tag="VII37_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5069576.1"
                     /translation="MSEFTGTDRTKVRRHPERGTYDREIIYKILDEAFVCHVGFIVDG
                     VPYVVPTNYVRGGDKLFLHGSTASRLMRTIGSGAPFCLSVTLLDGIVLSPTATGHSVN
                     YRSVVVMAKAEQIVDPQDKLAAMRDFVEYVIRGRWADVRPPTDQELKATMVLAVPLVE
                     ASAKVRTGFAVDKDNEYAEAGWTGVIPFKWAAQKPVPDPRGNAALPIPANLLNYSRPQ
                     "
     gene            3477..>4126
                     /locus_tag="VII37_07305"
     CDS             3477..>4126
                     /locus_tag="VII37_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY5069577.1"
                     /translation="MSVRSGIIFLFLFCGCSHAAGQERIAPPGVVKEEPPFRKKVYTD
                     KKGTKMPYRLFVPPGYDSSKKYALIFWIHGGSGRGSNNEAQISNENEKGSHVWITADN
                     QATFPALVLAPQCPERENWSSPEFNEVSGPLQLAMEIFALVQKDYSIDPDRIYLAGQS
                     MGGVGVWTLLQTYPELWAGAVVVSSYDNFTNVRAVTRVPLWLFQSEMDMTVPVDLVR"
BASE COUNT          927 a         1070 c         1257 g          872 t
ORIGIN      
        1 ccattcgcgg ttgtagcgct ctgaagtgtt ggtggcgctc gcgagttggg cgggggtttg
       61 tggtccgcct tttgcgagtg ctttatacag gcccaacttg tctccgatgc ggatcagcgc
      121 ggcattgaag gtggcgccaa aatcaacgat ggctctgccc agaaattcat ggagtttgtc
      181 ttgattgatg ctcataggac gctcctcggt gggtggacgt gtgggaactg acttggctgc
      241 attctaggag cctctgggca tgttcggcaa gtacagattt ttagcagcag cggttggggc
      301 gagagtagcg gcggttcact cgttcgacgt agaactcggc cggagtcagc aggcaggatt
      361 tttcaggctt cgttctttgt gcttggatat aacgttcgta ggcgttgtcg ccgcaccatt
      421 cgcgaattcc ctgccacagc ttgctggtca aacgtttcaa gcgtagtgca actataagac
      481 cacgcaggtt ctgcatgcga gccgctgtca tggtttgcgc ggattgcata ctcatccctc
      541 catcgcaggt gtttcgggtt cggccgctgt cgcggcgtag gcggcgccgg aaaagttctc
      601 tggccaacgt gtggccacgt aagccgtttc atgtaagacc gatttggggt ttttgctgag
      661 gatgcgatac cactgcatta gcgcttcgac gattagaaca ataatcatcg cggccagcac
      721 ggcggtcact gccgcatcca gccgctggtt gaaaattacg cgctgggtat cggcgatttt
      781 tgcggcagga attttgcccg cggcaatttg tgtggcaaga gccttggcgt aggagagaaa
      841 accgaggcgt ggattagggt cgaatatttt ctggtagctg gcggtcatgg tgatgatgac
      901 cagccaggtc atggggatca gcgtcaacca aatccagcgg atgcgcttca tcttcaacag
      961 aattgtagtg gccaccacca gggccacggc ggcgagcaac tggttcgaaa tgccaaataa
     1021 cggccacaac gaattgattc ccccgagcgg atcgacgacg ccctggtaca agaaatatcc
     1081 ccatgcgccg acgattagcg cgctcgaaag taaaattcct ggccaccatc cggttctcgc
     1141 gaagggcttc cacactcggc cgccaagttc ctgcaccata aaacggccaa cgcgcgttcc
     1201 ggcgtccagt acggtgagga tgaacagcgc ttcgaacatg atcgcgaagt ggtaccagat
     1261 gctgagcagg cgcggcccgc cgatggtgct cgaaaatatt tgcgccatgc ctacggccaa
     1321 cgaaggagcg ccgccggcgc gattcaagag ggtttgttct ccgacggcgc gcgccagatc
     1381 ggtcatggtt tgtgcctgca gagggtagcc ccaactgctg atggttgccg cggcggcatc
     1441 ggaggtcgcg cccacgatac tcgccgggct gttgatcgcg aaatatacgc caggggtcat
     1501 ggcgcaagcg gcgaccatgg ccataacgcc gacgaaagat tccatcaaca ttgcgccgta
     1561 gccaataaat cgcgcgtggc tttcgcgaat gatcattttc ggcgtggtgc cggaggaaat
     1621 gagggaatga aaaccgctga tcgctccgca agcgatggtg atgaaacaaa atggaaatat
     1681 ctttccagcg aataccgggc cgctgccgtc gatgaagcgc gtaagcggag gcatcagtac
     1741 ctgaggccgc accagcaaaa ttccagcagc caaggaaaaa atagctccgg ccttgatgaa
     1801 ggcgcttaaa taatcgcgcg gagcgaggag cagccatacc ggaagcgcgg acgcagcaaa
     1861 cccataggcg attagcgcga acgccagcgt tacgccgctc aaagtgaaga catgcgccca
     1921 ggaaggcgaa tccgccaccc actggcccgc gactaccaca aacaaaacga gagccacgcc
     1981 gatgaatgag gcttccagaa cgcggcccgg ccgtagaaat cgcagataaa gtccgaggag
     2041 taaagcgatg gggacggttg ccgccagcgt gaaggtggcc catggcgaag acttgagcgc
     2101 gtttacgatt accagtgcga ccacgccaag aagaatgacc atgatcgcga ggacggcaag
     2161 ttgggcaatg aatccgccac gttgactaac ttcttcgcgg gcgatttcgc ctagagtttt
     2221 tccgtcgcgc ctgatggagc agaaaagaat aatgaagtcc tggacgcatc cggcgaagac
     2281 ggctccggcg attagccaga gcgtgccggg caagtaaccg aattgtgccg ccaatgtggg
     2341 gccgaccagc gggcccgggc cggcgatggc ggcgaaatgg tggccaaaca aaatccactt
     2401 attggtgggc acgaagtcgc gtccatcgtc gtggcgctcg gagggagttg cgcgagtgtc
     2461 atccagcgca agcaggcgtg ccgcgatgaa cgcggaataa aagcggtagg cgaccaggta
     2521 agaacaccct gcggccacta caaaccaggt ggcatttagc ggttcgcccc ggtgaaaagc
     2581 aatcgtgcct agtgcggcgg ctccaatgag cgccacgacg atccagatga caacggtgag
     2641 gggccgcttc attccctggt caacaaattc cccggcggca tcgtatgcga gggcgtacga
     2701 agcgtcaatc agttctatgt taaatgcgga ttggctcttg atgcgcagga tgctcggagc
     2761 taggcttttg tgatgagcga atttactggc actgacagaa cgaaagtgcg ccgtcatccc
     2821 gagcggggta cttacgatcg cgagatcatt tataagatcc ttgacgaggc ctttgtttgc
     2881 cacgtgggct ttatcgtcga cggcgtgccg tatgtggtgc cgaccaatta tgttcgcggg
     2941 ggggataaac tatttttgca cggctccacg gcgagtcgac tgatgcgcac aataggcagc
     3001 ggtgcgccat tttgcttgag cgttacgctg cttgacggca tcgttctttc gccgaccgct
     3061 acgggccact cggttaatta tcgttccgtg gtggtgatgg ccaaagccga gcagattgtc
     3121 gatccgcaag acaagcttgc agcgatgcgc gactttgtcg aatatgtcat tcgtggacgt
     3181 tgggcggatg tgcgtccgcc aaccgatcag gagctgaagg ccacgatggt tctggctgtt
     3241 ccgctggtgg aagcgtcggc gaaagttcgt actggatttg ccgtcgataa ggacaacgaa
     3301 tacgcggaag ccggatggac cggggtgatt ccgttcaagt gggcggcaca gaagccggtg
     3361 ccggaccctc gcggaaatgc cgcgcttccc atacccgcta atttgcttaa ttactcacga
     3421 ccgcaataaa aaagatttcg ctgcgccaga tcatcgcgtc ttgaaagagg caacgcttgt
     3481 ccgttcgttc aggaatcatc ttcttatttt tattttgcgg ttgctcgcac gccgcaggcc
     3541 aggaaaggat cgcgccgccc ggcgtggtga aagaagagcc gccctttcgg aaaaaagtct
     3601 atacggacaa aaaaggtacg aagatgcctt atcgtctttt cgtgccgccc ggctacgatt
     3661 cctctaaaaa atatgccctg attttctgga ttcacggcgg cagcgggcgc ggctcgaata
     3721 acgaagcgca gatctccaat gaaaacgaaa aaggttcgca cgtgtggatt actgcggata
     3781 atcaggccac atttccggcg ttggtattgg cgccacaatg tcctgagcgg gaaaattggt
     3841 caagtccgga attcaacgaa gtaagcggcc cattgcaatt ggcgatggag attttcgcgc
     3901 tggtgcaaaa agattattcc atcgatcccg atcgaatcta tctggctggg caatcgatgg
     3961 gcggagttgg ggtctggacg ctgctgcaaa cctatccgga gctctgggcc ggggccgtgg
     4021 tagtttcttc ttacgataat tttacgaatg tgcgggccgt tacgcgggtt ccgctttggc
     4081 tttttcagtc agagatggac atgacggtgc cggtggatct ggtgcg
//
LOCUS       DATCBB010000661         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_21510, whole genome shotgun sequence.
ACCESSION   DATCBB010000661 DATCBB010000000
VERSION     DATCBB010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21510"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VII37_07310"
     CDS             <1..1877
                     /locus_tag="VII37_07310"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEY5069578.1"
                     /translation="AWAKKEKRLLITDTTFRDAHQSLMATRVRTHDLLATAGAVARRL
                     PNLFSLEMWGGATFDTSLRFLHEDPWQRLRDLRIRVPNICFQMLLRGANAVGYASYPD
                     NVIVEFVREAHAQGIDIFRIFDSLNSIDNMRVSIDAALETGAVCEPAICYTGDILDSA
                     RPKYSLKYYVGLAKQLEKLGAHFLAIKDMAGLCKPYAAYELVKTLRQEIGVPVHFHTH
                     DTSGLNAASILKAAEAGVDVADAAVASMSGGTSQPNLNAIVESLRHTPRDTQLDIETL
                     NECSDYWETVRNFYGPFDSGPKSGSARLYQHEIPGGQYTNLREQATSMGLGHRWHEAE
                     KMYAEVNQLFGDIVKVTPSSKVVGDMALFLMANELKPADILALDEKHDVSLPNSVVEM
                     FSGVLGVPPGGWPKKLQKIILRGEAPVHGRPGASMQAVDLDKEQKALEKKLNHSVRRD
                     DLLSYLLYPEVFTKYDKFRQTYGDVSVLPTYAFFYGLRSGEEITVEIEAGKSLIIRFL
                     TFSEPRPDGTRTLFFELNGQPRELNVRDRSLRVVERAHPKADPADQGQVAAPTAGVIS
                     GIVVQANQAVERGAKLLTLEAMKMQSNIYAPISGRISKLSVSSGQNVEAKDLLVTITP
                     "
BASE COUNT          443 a          494 c          615 g          481 t
ORIGIN      
        1 cggcttgggc gaaaaaagaa aagcggctgt tgatcacgga cacgactttt cgcgacgcgc
       61 accagtcgtt gatggccact cgtgttcgca cgcatgactt gcttgcgact gctggagctg
      121 tagcgcggcg gctgccgaac cttttcagcc tggaaatgtg gggcggcgcg acgtttgata
      181 cgtcgttgcg ctttttgcat gaagatccgt ggcaacgact gcgcgatctg cggattcgtg
      241 tgccgaatat ttgctttcag atgctgctgc gcggggcgaa tgcggttggt tacgcttctt
      301 atccagataa tgtgattgtg gagtttgtgc gtgaggcaca cgcacaaggg atcgacattt
      361 ttcgaatttt tgattcgctt aattccatcg acaacatgcg cgtttcgatc gacgcagcgc
      421 tggaaaccgg cgcggtttgc gaaccggcca tttgttacac gggcgacatt ctcgatagtg
      481 cgcggccgaa atattcgctg aaatattacg tgggactggc gaagcaactt gaaaagctgg
      541 gcgcccattt tctggctatt aaagacatgg ccggcttatg caaaccgtac gctgcttacg
      601 aactcgttaa gacgctgcga caagagattg gcgtaccggt tcatttccat acgcacgaca
      661 ccagcgggct caacgcagcc agtattttga aagctgctga ggctggagtc gacgtggcgg
      721 atgcggcggt ggcttccatg agcggcggca cgagccagcc gaatttgaat gccattgttg
      781 aatcgctgcg gcacacgccg cgcgacacgc agctcgacat cgaaactttg aacgagtgct
      841 ccgattattg ggaaacggtg cgtaactttt acggcccgtt tgattcgggg ccgaagtcag
      901 ggtctgcgcg cttgtaccaa cacgagattc ctggcgggca atataccaat cttcgggaac
      961 aagctacgtc tatgggcctg gggcaccgct ggcatgaggc ggaaaaaatg tatgcggagg
     1021 tcaaccagct ttttggcgac atcgttaaag tgacgccgtc cagcaaggtg gttggcgaca
     1081 tggcgctttt tctgatggcc aacgaactga agccggccga tattctggcg ttggacgaga
     1141 agcacgacgt ttcgctgccg aattcggtgg tggaaatgtt ttccggcgtg ctgggtgtgc
     1201 cgccgggcgg ttggccgaag aaactgcaga aaattatttt gcgcggtgaa gctcccgtgc
     1261 acggccggcc tggtgcaagt atgcaggctg tggatttaga caaggagcaa aaagcgctgg
     1321 agaagaaact gaatcattcg gttcggcggg atgacctctt aagctacctg ctgtatcccg
     1381 aggtgttcac caagtatgac aagttccggc agacatacgg cgatgtgagc gtacttccta
     1441 cgtatgcttt tttttatggc ttgcgatctg gggaagaaat tacggtagag atcgaggctg
     1501 gaaaatcact gattatcagg ttcctgacgt ttagcgagcc gcgtcctgat ggcacgcgca
     1561 cgttattttt tgagttgaat ggccagccgc gggagctgaa tgttcgcgat cgctcgttgc
     1621 gggttgtgga gcgggcgcat cccaaggcgg atccagctga tcagggccag gtcgcggctc
     1681 ctaccgcggg cgtcatcagt gggattgttg tgcaggccaa tcaggcggtg gagcgcggcg
     1741 cgaaactact gacgctggag gccatgaaga tgcaatccaa tatttacgcg cctatttcag
     1801 gacgaatctc caagttgtcg gtctcctcgg ggcagaatgt ggaggccaag gatttgctag
     1861 tcacaattac tccatgacct ttcgcaagca cgagaaatgt ccttccaaag ttttgaaaaa
     1921 tgaacgagac ctggtggtat acctgccacc cggctacgag gatcaacctt cgcggcgctt
     1981 tccggtgctg tatttacacg atgggcaaaa cctttttgat ggttccacag cgt
//
LOCUS       DATCBB010000662         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_477094, whole genome shotgun sequence.
ACCESSION   DATCBB010000662 DATCBB010000000
VERSION     DATCBB010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477094"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /gene="priA"
                     /locus_tag="VII37_07315"
     CDS             <1..1965
                     /gene="priA"
                     /locus_tag="VII37_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY5069579.1"
                     /translation="SLLARKHKTQTILAWRTEDSEKRKKSADSPGQHRTRPLQGKALE
                     KFQEQTRRVRELLEAERGPLPLSQVLRLADLSRGAVDRMLASGLLYSWEEPIDPAEDP
                     FDVGYAPPAHELNAEQDMALKRIRARFELGEFGVQLLYGVTGSGKTEVYLRAIQETLA
                     RQKTALVLVPEIALTLWVGRQCRAWFGARFEGVAVLHSALTDVERAREWWRVRNGEAR
                     VVVGTRSAVFAPLERLGLIIVDEEQENSYKQEETPRYHGRDVAIVRAKMENAVALLGS
                     ATPSLETYHHATTGKYELLTLGSRVENRALATVEVVDLREEFQQTHQASSISATLRAG
                     IQECLDGRAQALVLINRRGYSWSVLCRSCGASVQCANCSISMTYHKNRNRLECHYCGF
                     IAQVPKNCPKCDSKYVYFFGEGSEHLEERLRKEFPTARIARLDRDTTRTKRQYQETLG
                     AFAGGALDILVGTQMLAKGHDFQRVTLVGVISADGLLSMPDFRAAERTFQLLTQVAGR
                     AGRGELHGRVLIQTYYPEHYAIQDAVKQEYLAFFEREANFRRTMGYPPFTSLANVIVR
                     NTNLEKAIRWSRQLSEYFSPHNGKGVRILGPATAPLARLKKEFRFQFLLKSPKRSLLT
                     KLLAGALDHCEANEIPDTAVIVDMDPLSLM"
BASE COUNT          467 a          498 c          672 g          425 t
ORIGIN      
        1 agcttgcttg caagaaagca taagacgcag acaattcttg cctggcgaac tgaggattcc
       61 gaaaagcgga aaaagagcgc ggattcgccg ggacaacaca ggacgcgccc gctgcaagga
      121 aaagcgctgg agaaatttca ggaacaaacc aggcgggttc gcgagttgtt ggaggccgaa
      181 cgcgggccgc tgccgctgtc ccaggtgttg cgattggctg atctttcgcg tggcgcggtt
      241 gatcgcatgc tcgccagcgg gttgctctac agctgggagg agcctattga tccggccgag
      301 gatccttttg atgtgggata cgcgccgccg gctcacgaat tgaatgctga acaggatatg
      361 gcgcttaaac gcattcgtgc gcgattcgaa ttgggggaat ttggtgtgca gttgttgtac
      421 ggcgtgaccg gtagcgggaa aacagaagtg tatttgcgag cgattcaaga gacgctggcg
      481 cggcaaaaaa cggcgttggt gctggtgcca gagatcgcgc tgacgttatg ggtggggcgg
      541 caatgccggg cgtggtttgg cgcgcgattc gaaggagtgg cggtgctgca ctccgctctt
      601 accgatgtgg agcgcgcgcg ggaatggtgg cgggtacgaa acggtgaggc ccgcgtggtg
      661 gtgggcacgc gctcggcggt atttgcgccg ctggagcgac tcgggctaat catcgtggac
      721 gaggagcagg agaacagcta caagcaggaa gagacgccgc gatatcacgg gcgggatgtg
      781 gccatcgtga gggccaaaat ggaaaacgcg gtggcgctgc taggctcggc caccccttcg
      841 ctcgaaacct atcatcacgc cacgaccggg aaatacgagt tgttgacgtt ggggtcgcgg
      901 gtcgagaatc gcgcgctggc gacggtggaa gtcgtcgatt tgcgggaaga atttcagcag
      961 acccatcaag ccagttcgat ttccgccacg ctgcgtgcgg gaattcagga atgcctagat
     1021 ggccgcgcgc aggcgctggt gttgatcaac cggcggggat attcctggtc ggtgttgtgc
     1081 cgcagttgtg gagcttcggt gcaatgcgcg aactgcagca tttccatgac gtatcacaag
     1141 aacagaaatc gcctggagtg ccactactgc ggatttatcg cacaggtacc gaaaaactgt
     1201 ccgaagtgcg attcgaaata tgtttatttt tttggggaag gttcggagca tttggaagaa
     1261 cggttacgta aagaatttcc gactgcacgg attgcgcggc tggaccgcga caccacgcgc
     1321 accaagcgcc aatatcagga aacgttagga gcgttcgcag gcggggcact cgacatcctt
     1381 gtagggacgc agatgctcgc gaaggggcac gattttcaac gtgtgacact ggtgggcgtg
     1441 atctccgcgg acgggttact aagcatgccg gattttcgcg cggcggagcg cacctttcaa
     1501 ttgttgacgc aggtcgctgg acgtgccggc cggggagagc ttcacggaag ggtgctgatt
     1561 caaacgtatt atccggagca ttacgcgatt caggacgcgg taaaacagga atacctggcg
     1621 ttctttgagc gtgaagcgaa ttttcgcaga accatgggat atccaccgtt cacgtcgctg
     1681 gccaacgtaa tcgtgcgcaa tacgaatctg gagaaagcga ttcgctggtc gcgtcaactc
     1741 tcggaatatt tttcaccaca caatggtaag ggcgtacgga ttctgggtcc ggcgacagcc
     1801 cctttggcgc gcctgaaaaa ggaattccgc tttcaatttc tgctgaaatc gccaaagcgc
     1861 tcgctgctga ccaaacttct ggccggcgca ctcgatcact gcgaggctaa cgaaatcccg
     1921 gatacggcag tgattgtgga catggacccg ttgagcctga tgtaacgatg gggctaggcg
     1981 gaagtttgtt ttcttcgcca gaaataatag gctgggagtc caagcaaaaa aattacgata
     2041 gctacagcca aatagtggaa ct
//
LOCUS       DATCBB010000663         1240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_152078, whole genome shotgun sequence.
ACCESSION   DATCBB010000663 DATCBB010000000
VERSION     DATCBB010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152078"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..792)
                     /locus_tag="VII37_07320"
     CDS             complement(4..792)
                     /locus_tag="VII37_07320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5069580.1"
                     /translation="MNSEWRGKWALITGASAGIGVALAKELAAGGANLVLTARRQDRL
                     EQLANDLSGQHKIKTSIFAADLAEASAPEKIYAFTRAQGISIELLVNNAGFGAYGELP
                     SVEVSRLLDMVQVNCAAVVHLTRLYLPEMVTRRSGDILILASTAAFQAVPYISTYAAT
                     KAFDLLFAEGLAEEMKPHGIRVCALCPGSTESEFHVVAGQEKFMRKAETAEKVARTGL
                     KALAAGKSYVISGLGNYLGVQGERLVPRRMVTRVAAGMFKPRER"
BASE COUNT          284 a          364 c          312 g          280 t
ORIGIN      
        1 tattcaacgc tcgcgaggct taaacatacc cgcagctacg cgggtgacca ttctgcgcgg
       61 caccaggcgt tcgccttgca cgccaagata atttcccagt ccagagatca cgtaactctt
      121 gccggccgcc aaggccttca gtccggtgcg cgctactttc tccgccgttt cggctttacg
      181 cataaatttt tcctggcccg ccaccacgtg aaattccgac tccgtcgagc ctgggcaaag
      241 cgcgcaaacg cggatgccgt gaggcttcat ttcttcggcc aaaccttctg cgaagagtaa
      301 gtcgaatgct ttagtcgctg cataggtgga aatgtacgga acggcctgga acgcagccgt
      361 cgaagccaga atcaaaatgt caccactgcg ccgcgttacc atctccggca aatacagtcg
      421 cgtcaaatgc accacggcag cgcagttgac ttgcaccatg tccagcaagc ggcttacttc
      481 aacggaaggc agttcgccgt aggcgccgaa acccgcattg ttcaccagaa gttcaatgga
      541 gattccttga gcgcgcgtga aggcataaat cttttccggc gcgcttgcct ccgccaggtc
      601 cgccgcaaat atggaagttt taattttgtg ctgcccgctg agatcgttcg ccaattgttc
      661 aagtcgatct tgccgccgcg cagtcagaac gagattcgct ccgcccgccg ccaattcctt
      721 tgctagcgcc acgccaattc ctgcgctggc cccggtaatc aacgcccatt ttccacgcca
      781 ctctgaattc atgaagctgt ccctcctgct ggcgctcgct gcacgcggca tgtcagccag
      841 cttacttgaa tgcccgcaga attgcggtgg atacgcacgc gggcctagcg gtagatcgat
      901 tttagaattc gcctaggatg caacaaaaaa tacctcgtgg acttgttttc aaaatcatgg
      961 aaaccccaaa actcgtgcaa aaccttcttt tccagaggga taggggagcc caaatgtgca
     1021 caaggggtta atactgtaac cgtatgggtg ggtcaaatca gtactaaggt cccattctga
     1081 aacacttctc tgccacctaa aaacgtactg atagtactag ctacgtcccc cgtgtggccg
     1141 tcggctaggt tccttgaatg cgtttacgcg cagtgtgacc aggtggctgg atgggccacg
     1201 gagaagaaat gccgctagat cagcttgatc agcaaatggt
//
LOCUS       DATCBB010000664         1275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_216407, whole genome shotgun sequence.
ACCESSION   DATCBB010000664 DATCBB010000000
VERSION     DATCBB010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216407"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VII37_07325"
     CDS             complement(<1..927)
                     /locus_tag="VII37_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY5069581.1"
                     /translation="MARKSGSTISQDELYPASEVRAYGRGNGREDAREELDETRLLDL
                     DAEQESPFLRGQKRVSVRRGTIPKKTAAGLAWTALAICVALFCIAGVAALYHYGEHSW
                     RFRIESSDDIDITGIHNVTRPQILEIMGADIGRNIFFIPLGQRKAQLEQIPWVQAASV
                     MRFVPNRIAVQITERTPVAFARIGSKIFLLDDAGTPMDLSATKKKYSFPVILGMNAGE
                     PPSTRKARMKIYNELVSQLDSAGAHYSQDLSEVDLSDLDDVKVLANNADGNVLVHLGS
                     SQYLERYKVYVAHVKEWRQQFAKVDSVDLRYDR"
     gene            complement(1085..>1275)
                     /locus_tag="VII37_07330"
     CDS             complement(1085..>1275)
                     /locus_tag="VII37_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069582.1"
                     /translation="STGDLVAQNVSTFQDAAASVAAIAKPGDMILTLGAGSVGQLAPM
                     ILEKLRATNIAASIADSI"
BASE COUNT          285 a          341 c          340 g          309 t
ORIGIN      
        1 ccggtcatat cgaagatcga ccgaatctac ttttgcgaac tgctgccgcc actctttcac
       61 gtgagcgacg taaactttat agcgctccaa atactgtgac gaaccgagat ggaccagaac
      121 gtttccgtca gcattgttgg cgagaacttt tacatcatcc agatcggaga ggtccacttc
      181 gctaagatct tgtgaataat gcgcgcctgc tgaatcgagc tggctcacca gttcgttgta
      241 aattttcatc cgggctttgc gggtggaggg aggttcgcct gcattcatcc ccaggatgac
      301 cggaaatgaa tatttcttct tggtcgccga taaatccatt ggagtacccg catcgtcaag
      361 caggaaaatc ttcgagccaa tgcgcgcgaa tgcgacaggc gtacgctccg ttatctgcac
      421 cgcaatgcga ttaggaacaa atctcatcac gctggcggct tgcacccatg gaatttgctc
      481 caattgggcc ttccgctggc ccaacggtat gaaaaatatg ttgcggccga tatcggcgcc
      541 cataatttcc agtatttgcg gccgcgtaac gttgtgaatg ccggttatat cgatgtcatc
      601 gctggactcg atgcgaaatc gccatgaatg ttcgccgtaa tgataaagag cagcgacgcc
      661 cgcgatgcaa aagagcgcga cgcagattgc cagcgctgtc cacgccaaac ctgccgcggt
      721 ttttttgggg atggttccgc ggcggacgga gactcgcttt tgtccgcgca ggaatggaga
      781 ctcttgttcc gcatccaggt cgagcagccg cgtttcatcc aactcttcgc gggcatcttc
      841 acgaccattt ccgcggccgt aagcgcgcac ttcggacgct ggatacaact cgtcctgcga
      901 gatggtggaa ccgcttttcc ttgccatcaa atcaggtcag ttgtcgtcag cgaatcgcga
      961 gcaaatttac tataacggct ttttgcgatg ggagataggg atagtttggt tcgtttttac
     1021 gtggaagggc tgaatcattc ccgatctaca tcgcgtagac cgccattgcc aattggggga
     1081 acagctaaat cgaatcggca atgctcgctg cgatgttcgt ggcccgaagt ttctccaaaa
     1141 tcatcggcgc gagttggcct acgcttccag caccgagtgt aagaatcatg tcgcccggtt
     1201 tggcaattgc cgctacagag gcggctgcat cctgaaacgt gctgacattc tgcgcaacaa
     1261 ggtcgccggt cgacg
//
LOCUS       DATCBB010000665         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_280769, whole genome shotgun sequence.
ACCESSION   DATCBB010000665 DATCBB010000000
VERSION     DATCBB010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280769"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VII37_07335"
     CDS             complement(<1..671)
                     /locus_tag="VII37_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5069583.1"
                     /translation="MTTTLIHAKRALTPTGEIADAGILMRDGVIELIGPREGMNLPND
                     AAEISATDKSAIPGFVDVHIHGAGGRDVMEGTPEALSVVAKTLALHGTTSFVATTVTA
                     SADDICRSSEGIAKYIATQHEPHEPRAEVLGIHFEGPFLSEIRRGVHPAEWLRLPSAE
                     LLSRFLAAAGGNARILTLAPELLGAMPCIDAAIKAGVVVGIGHTDATYEQARAAIAHG
                     AHHAV"
     gene            complement(889..1734)
                     /locus_tag="VII37_07340"
     CDS             complement(889..1734)
                     /locus_tag="VII37_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069584.1"
                     /translation="MSEYQEPIVVQTAEAPRWVALAVAVVAILSLIGLGVGWSAISHA
                     NSVEQSTQASVKQTNDTLGQRLAKAEEQNQQLQSDLKVVTNKLNVTQSELIAARKQTK
                     SATVAYEKKLNGLQSSVSSELATKASADDVNRLNGDVTGVKGDLDATKSSIQMARSEM
                     GTLIARNHDEIDQLRRMGQRDYFEFALTRKSGPQKVGGIQVQLKSTNVKKNRYTVDVL
                     ADDKSFEKKDRSVNEPIFFYTGGSRAALELVINKVTKTGATGYLSLPKSAGATTAASA
                     SAASS"
BASE COUNT          393 a          561 c          565 g          481 t
ORIGIN      
        1 tgcaccgcgt gatgcgcgcc gtgcgcaatc gcggcgcgag cctgctcgta agtagcgtcg
       61 gtatggccga tgccaacgac cactcctgct tttattgcgg cgtcgatgca gggcattgct
      121 ccaagtaatt ccggcgcgag cgtgagaatt ctcgcgtttc cacccgcagc agcgagaaat
      181 ctggatagca attccgccga aggaagccta agccactcgg cgggatgcac gccgcgcctg
      241 atttcgctca agaagggccc ctcaaaatgt atacccagca cttccgcacg aggttcgtgg
      301 ggttcgtgct gggtggcgat atatttggcg atgccctcgg agctgcgaca aatatcatcg
      361 gcgctggcgg ttactgtggt ggccacgaaa gaagtggtgc cgtggagagc gagcgtctta
      421 gcgacaacgc tcaacgcttc cggggtacct tccatcacat cgcgtccgcc agccccgtgg
      481 atatgcacgt cgacgaatcc gggaattgcg gatttatcgg tggcggagat ttcagcagca
      541 tcgttgggta gattcatccc ctcgcgtggc ccgatcagtt cgatcacgcc gtcgcgcatc
      601 agtattcccg catccgcgat ttcgcccgtg ggcgttagcg cccgtttggc gtgaatcaga
      661 gtggtcgtca tggcagccat acatcgtagc ggaaagattg cgtcacacaa aactcgcaga
      721 aagccaagct tgatttgcgc gcttaaagcc aaggcgggag acggttgggg tttacttcct
      781 aagagtgtgc ggctcaaaca aaaaggagga ctcattagag tcctcctttt cgtgatcaga
      841 atccgactct caggcggaac tgatctctgt tgcaatatgg ttataaattt agctgctcgc
      901 cgcgctggcg ctagctgcag tggtagctcc cgcggacttg ggaaggctca ggtagccggt
      961 ggctccggtc ttggtaactt tgttgattac cagttcgagc gccgcgcggc tcccgccggt
     1021 atagaagaag atgggctcgt tcaccgaacg atccttcttc tcgaagctct tgtcatcggc
     1081 cagcacatcg acggtgtagc ggttcttttt tacgttggtg gatttcagct ggacttggat
     1141 accgccgact ttttgcggtc cactcttgcg cgtcaacgca aattcaaaat aatcgcgctg
     1201 gcccatgcgg cggagttgat caatttcatc atggttacga gcgatgagcg tgcccatttc
     1261 gctgcgcgcc atttggatgc tgctcttggt ggcgtccaga tcgcctttca cgccggtgac
     1321 atcgccattc agcctgttaa catcgtcagc gctcgccttg gttgccaatt ccgaactcac
     1381 ggagctctgc aggccattga gtttcttctc gtacgctacg gtagcggact tggtttgctt
     1441 gcgcgctgcg atcaattccg attgcgtcac gttgagtttg ttggtgacca ctttcaaatc
     1501 gctttgaagc tgttgatttt gctcttcggc tttcgctaag cgctgtccca acgtgtcgtt
     1561 ggtctgcttc acagatgctt gtgtggattg ttcaacgctg tttgcgtggc tgatagcgct
     1621 ccaacctacg cccagcccaa tcaaagacaa aattgcgacg accgccaccg ccaaggccac
     1681 ccagcgcggg gcttcagctg tctggacaac tatcggttcc tgatattcgc tcatttggac
     1741 gtctcctctt cggcgggcac ccacccgggc tcgccacata catggaacac tgcatacggg
     1801 cggccaacgt ccctgtatat ctgcggtaga gtcaagacca gcggctgcag gcacataggc
     1861 gggaatgcat cagtggcagg gcgaggccta atcgtactag tactggaaag tcttacacgc
     1921 tctattaccc acacgtaaaa tttgctgacc aagtgtcatt tcgtggggtg cattcgcgcg
     1981 aggcgaatcc acccgccggg
//
LOCUS       DATCBB010000666         1060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_477302, whole genome shotgun sequence.
ACCESSION   DATCBB010000666 DATCBB010000000
VERSION     DATCBB010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477302"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VII37_07345"
     CDS             complement(<1..635)
                     /locus_tag="VII37_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069585.1"
                     /translation="MNRVNLVVLWHMHQPQYRDPETERYVLPWTRLHATKDYFGMVKV
                     LEEFPGFHATFNVVPALGQQLEEYASGNFKEPWFSLAFKNAEKLTKEDKTEILARAFQ
                     VNHERLMSRWKRFVELYEWSRPADGAQAKVTFTVRDWRDLQVLSQLAWMEESWLEKDE
                     VVSRLASKGRDFTESDKEALKAKEIELIGLVLPAHREAAQRGQIEISTTPF"
     gene            692..>1060
                     /locus_tag="VII37_07350"
     CDS             692..>1060
                     /locus_tag="VII37_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY5069586.1"
                     /translation="MRILDRYIVREVFRHALLGLIVFTFVFFVPQLIRLMELFVRHTG
                     SGSQILKLFLCIFPAVFTFTIPMAVLIGVLLGLGRMSADSEIIALTALGIGRRRILLP
                     VGVLAIAGLIVSLTMTLWLSP"
BASE COUNT          197 a          361 c          222 g          280 t
ORIGIN      
        1 taaaaagggg tggtgctgat ctcgatttgg ccgcgctgtg cggcttcgcg gtgcgccggc
       61 aacaccaatc caatcagctc aatttccttg gccttcagcg cctctttatc gctttctgtg
      121 aaatctcttc ccttcgacgc cagcctgctc accacttcat ctttctcgag ccaggactct
      181 tccatccacg ccagttgcga tagcacctga agatcgcgcc aatcccgcac cgtaaacgta
      241 actttggcct gcgcaccatc cgcgggccgc gaccattcat acaattccac aaagcgcttc
      301 catcgcgaca tcaaccgctc gtgatttacc tgaaaagcac gtgccagaat ctcggtcttg
      361 tcttctttgg tcagcttctc ggcattcttg aacgccagcg aaaaccatgg ctccttaaaa
      421 ttgccgctcg catattcctc caactgctgt cccagcgccg gaaccacatt aaatgtcgcg
      481 tgaaatcccg gaaactcctc cagcactttc accattccaa aataatcttt cgtcgcatgc
      541 agccgcgtcc acggcaacac ataccgctcg gtttccggat cgcgatattg cggctggtgc
      601 atgtgccaca acaccaccag gttcactcgg ttcatcttct acaccgctcc tgtctcaatt
      661 cttcgcgcgt gttacactca ccgttcgcca catgcgcata ttggaccgtt acattgtccg
      721 cgaagttttt cgccacgcgc tcctcggact catcgtcttc acctttgttt tcttcgtacc
      781 tcagcttatt cgcctgatgg agctgttcgt tcgccacacc ggctccggct cccaaatcct
      841 taaacttttc ctctgcattt ttccggccgt cttcaccttt accattccca tggccgtact
      901 tatcggcgta ctgctcggac ttggccgcat gtctgccgac agcgaaatca ttgcgctcac
      961 cgccctaggc atcggacggc gccgcatcct gctcccggta ggcgtgctgg ctatcgccgg
     1021 gttgatcgtg agtctgacca tgactctctg gctttcccct
//
LOCUS       DATCBB010000667         1016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_85996, whole genome shotgun sequence.
ACCESSION   DATCBB010000667 DATCBB010000000
VERSION     DATCBB010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85996"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1016)
                     /gene="tyrS"
                     /locus_tag="VII37_07355"
     CDS             complement(<1..>1016)
                     /gene="tyrS"
                     /locus_tag="VII37_07355"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY5069587.1"
                     /translation="AYLKKGSAEIIREAELQTKLEKSAKTGKPLRVKLGVDPTAPDIH
                     LGHTVVIRKLKHFQEMGHTAVFLIGDFTAMVGDPTGQSETRPPLTREQVNANAETYLE
                     QVFKILDREKTEIRYNSEWLDKLSSYDIVRLCAKYRVARMLEREDFHTRLTNQQPISM
                     HELLYPLLVAYDSVVLESDVELGATEQKFNLLMGREIQREYGQESQVAFTMPILVGLD
                     GSRKMSKSLGNYVGITETPDQIFGKMMSIPDELMWSYYELVTDRTPEEITKLKSDVSN
                     GTTHPMDIKMLLAQEVVSGFHNEAAGRKASEMFQRVFRDRQAPEEAPVKNVAAGTPQM
                     IKTL"
BASE COUNT          209 a          281 c          269 g          257 t
ORIGIN      
        1 caaggtcttg atcatttgcg gcgtgcccgc cgcgacattt ttaacgggcg cctcctcggg
       61 cgcctggcga tcgcgaaaaa cgcgctgaaa catttcggac gctttgcggc cagcggcctc
      121 gttgtgaaaa ccgctcacca cttcctgcgc cagcagcatc tttatatcca tggggtgcgt
      181 agttccgttg gagacatcgc tcttcaactt cgtgatttct tcgggcgtgc gatccgtgac
      241 caactcgtag taagaccaca tcaactcatc cggaatagac atcatcttcc cgaagatttg
      301 gtccggcgtt tcggtgatgc ctacataatt gccgagactc ttggacattt tgcgcgagcc
      361 gtcgagtccc acgagaatcg gcatggtgaa ggccacctgc gattcctggc cgtactcgcg
      421 ctgaatttcg cggcccatca agagattgaa tttttgttcc gtagccccga gttccacgtc
      481 agattcgagc accaccgagt cgtaggcaac cagcagcgga taaagcaatt catgcatgga
      541 gattggctgc tgattggtta gccgcgtatg aaaatcctcg cgctccagca tccgagcgac
      601 gcggtatttt gcgcacagcc gcacgatatc gtagctggac aatttatcga gccactcgct
      661 gttatagcga atttcggttt tctcccgatc cagaatcttg aagacctgct ccagatacgt
      721 ttccgcattg gcattcactt gctcgcgagt gagcggaggg cgcgtttcgg actggccagt
      781 tggatcaccg accatggcag tgaaatcgcc gattagaaaa actgcggtgt ggcccatttc
      841 ctgaaaatgt ttcaattttc ggatcactac ggtgtggccc aggtgaatgt ccggtgcggt
      901 aggatcgacg cccagcttca ctcgcaaggg cttgccggtt ttagcggatt tttcaagctt
      961 ggtctgcagc tccgcttcgc gaataatttc cgcgctgccc ttttttaggt aagcga
//
LOCUS       DATCBB010000668         1042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_378044, whole genome shotgun sequence.
ACCESSION   DATCBB010000668 DATCBB010000000
VERSION     DATCBB010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378044"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VII37_07360"
     CDS             <1..884
                     /locus_tag="VII37_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY5069588.1"
                     /translation="VIAALAEAATNDKTWGVRAVAAESLGEIGGPAAAKKIIEALNSA
                     KEPWLRARMVAALGNFKDDKDVFAKLQTVAEQDTSYRARAAALQALGKLKAPNAFAIL
                     SASITADSPDDFLRNAALRSLGPLNDEKAVPVLREWVIPGKPMETREAAIFSLARLQK
                     DNKEITSQIASYLPEHHFGVRMAALAALGARGDASAIPALEQLLKSEDLSIEVAPMIK
                     EQIAHLKRPAKEKKEGHATAGTDSDDDDAEGSADSNSGGSRDTVVAERLDKLEHLIQE
                     MNSRLKTIETRLPTEKR"
BASE COUNT          264 a          254 c          313 g          211 t
ORIGIN      
        1 atgtgatcgc ggccttggcg gaggccgcga ctaacgataa aacctggggc gtgcgagccg
       61 tggccgccga gagtcttggc gagattggag ggccggctgc agccaagaaa ataattgaag
      121 ctttaaattc tgcaaaagaa ccatggctgc gggcgcgcat ggtcgcggcc ttgggcaatt
      181 tcaaagatga caaagatgtt ttcgcgaagc tacaaaccgt cgcggagcag gatacttctt
      241 atcgcgcgcg agcggcggca ctgcaggcgc tcggaaaatt aaaagctcca aacgcgtttg
      301 caattctaag tgcgtcaatc accgcggatt cgccggacga ttttcttcgt aatgcggcat
      361 tgcgctcgct gggcccgttg aatgacgaga aagctgtgcc ggtccttcga gagtgggtaa
      421 ttcccgggaa gccgatggag acacgagaag cggcaatctt cagcctcgcc cggctgcaaa
      481 aagataacaa ggaaattacc agccagatcg cgtcgtattt gccggagcat cactttggtg
      541 ttcgcatggc cgcgttggcc gcgttgggcg cgcgcggaga tgcttcggcg attcctgctc
      601 tggagcagtt gctcaaaagc gaggacctga gcatcgaggt tgctccgatg atcaaagaac
      661 agatcgcgca cttgaaacgg cccgcgaaag aaaaaaagga aggccatgcc actgctggca
      721 cggatagtga tgatgacgat gcggaaggat ctgccgacag caattccgga ggtagcaggg
      781 atacggtagt cgccgagcgc ctggataaac ttgaacacct tatacaggaa atgaacagcc
      841 gtctgaagac tatcgaaaca cgcctcccga cagaaaaacg ttagtcagga tttgccgttc
      901 aggattcgaa gttcaaagtt ctgcagagga agattttgtg tttggtgcgg cgatcaagat
      961 tcgcaaatcc cgcagattgt ttccggtcgg cccggtgatg atcgcgtcat ccaaacactt
     1021 gaaaaatgtg aatgcgtcgc tg
//
LOCUS       DATCBB010000669         1327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_442696, whole genome shotgun sequence.
ACCESSION   DATCBB010000669 DATCBB010000000
VERSION     DATCBB010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442696"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /gene="trpE"
                     /locus_tag="VII37_07365"
     CDS             <1..1316
                     /gene="trpE"
                     /locus_tag="VII37_07365"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY5069589.1"
                     /translation="LETRNRLVWEERDPVSFLRQRMEKFKPVRIPGLPPLVAGAIGYF
                     SYDMVRLIERLPKTLRDEIGLYDAVLMFYHGILAFDHVQHRLWIVRNVFTEGPGTLRA
                     KYNAAVREIKRTRALLEEPAPAELVRGAAKKKARPLKVSSNFRKPEYLAAVRKAKAYI
                     RAGDIFQVVISQRFSAKVQAEPFEIYRELRALNPSPYLFYLRMNDVDVVGSSPEMLVK
                     VQGRDVFYRPIAGTLPRGRDEAEDQRFEKQMLASEKERAEHVMLVDLGRNDLGRVCEY
                     GSVKVEQLMLVERFSHVMHLVSSLKGRLREDVDCFDALMACFPAGTVSGAPKVRAMEI
                     IEELERTRRGIYAGGVLYMDFAGNLDSCIALRTMVIKNGMAHVQAGGGIVADSTPIGE
                     YQETLNKSRALLTALEAAHLSKGSATALAARVKKPRGTRGGRKLK"
BASE COUNT          302 a          285 c          443 g          297 t
ORIGIN      
        1 tgctggagac ccgcaatcgc ctggtgtggg aagaacgcga tccggttagc tttctgcggc
       61 aacggatgga gaagttcaag ccggttcgaa tacccgggtt gccaccgctg gtggccggag
      121 ccatcggata ctttagttac gacatggtgc ggctgatcga gcggctgcca aagactctgc
      181 gagatgaaat cggtttatat gatgcggtgt tgatgtttta tcacgggata ttggcgttcg
      241 atcacgtaca acaccggttg tggattgtgc ggaatgtgtt tactgaaggg ccgggaactt
      301 tgcgcgcgaa atacaacgca gcggtgcggg aaataaagcg gacgcgggca ctgctggaag
      361 aacccgctcc agccgagctg gtgcgggggg cggcgaagaa aaaagcgcgg ccgttgaagg
      421 ttagttccaa ttttcgaaag ccggaatatc tcgccgcggt tcgaaaagca aaagcttata
      481 ttcgcgccgg cgatatcttc caggtggtca ttagccaaag attttccgcg aaggtgcagg
      541 ccgagccatt cgagatttat cgcgaattgc gggcgttgaa tccttcgcca tatttatttt
      601 atttgcgtat gaatgatgtt gacgtggtgg gaagttcgcc ggaaatgctc gtgaaggtac
      661 aggggcgcga tgttttttat cgtccgattg ccgggacatt gccgcgcggg cgtgatgaag
      721 cggaagacca gcgtttcgaa aagcagatgc tggctagcga gaaagaacgc gcggaacatg
      781 tgatgctcgt ggatttggga cgcaatgacc tggggcgcgt gtgcgaatac ggcagcgtca
      841 aagtagaaca actgatgttg gtggaaagat tttcccatgt catgcatctg gtttcgagct
      901 tgaaggggcg tttgcgtgaa gatgtggatt gcttcgatgc gctgatggct tgttttccgg
      961 cgggtacggt gtcgggcgcg ccaaaagttc gagccatgga aattatcgag gagttggaac
     1021 gcacgcggcg ggggatttat gcgggtggcg tgttgtacat ggattttgca gggaacctgg
     1081 attcatgcat cgcgctgcgc accatggtga tcaaaaatgg aatggcgcac gtgcaagctg
     1141 gcggcgggat agtggcggat tccactccca ttggggagta tcaagaaacg ttgaataagt
     1201 cgcgagcgtt gttgaccgcg ttggaagccg cgcatctaag taagggcagc gcaacggcgc
     1261 tggccgcgcg cgtgaaaaaa ccgcgaggca cgcgaggcgg gagaaaactg aaatgaatct
     1321 cctgctc
//
LOCUS       DATCBB010000670         5381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_152390, whole genome shotgun sequence.
ACCESSION   DATCBB010000670 DATCBB010000000
VERSION     DATCBB010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152390"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VII37_07370"
     CDS             complement(<1..499)
                     /locus_tag="VII37_07370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY5069590.1"
                     /translation="MKLILASASPRRAEILRDAGMTFTVMSSAVDETPIPGETPNDYI
                     HRLACAKVELVAARSVGPAIVIGADTVVVLDGQIIGKPRSAEDARRTLELLSGRTHSV
                     VTGVMLVRLPDAERRGFVETTQVQFSAISPEEISRYLATDEPFDKAGAYGIQGRAGRY
                     IPRIEG"
     gene            complement(584..2233)
                     /locus_tag="VII37_07375"
     CDS             complement(584..2233)
                     /locus_tag="VII37_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5069591.1"
                     /translation="MADSNSKPVSSAARRSDRKEKFTTLSGLPLEALYTAEDLASRNR
                     EETIGYPGEFPYTRGIYPTMYRGRFWTMRQYAGFASAAESNERYRYLLGKGQVGLSVA
                     FDLPTQIGMDSDHPLAHGEVGKGGVAIDSIEDMEILFDRIPLEKVSTSMTINSTAAIL
                     LCLYVAVAKKQGASLAKLSGTVQNDILKEYIARGTYIYPVRSAMRIVTDIFSWCSEQL
                     PKWNTISISGYHIREAGSTAIQEVAFTLGDGMAYVQAALDAGLNVDDFAPQLSFFFNA
                     HNDLLEEIAKFRAARRLWARIMRERFHAKDPRSWLMRFHAQTAGSSLTAQQPENNIVR
                     VAIQALAAVLGGCQSLHTNSLDEALALPTEDAALIALRTQQIIAHETGVTNSIDPVAG
                     SYAIEQLTNEIEAGAQMYIDKINAMGGPLNGTLRAIESGFIQGEIQKAAYEFQRAVEK
                     NEQIVVGVNDFRVEEERPITILRVEEQIARDQIERLKAFRLKRDATRVKTVLAELARR
                     ASGAENLLPAILDAVENNCTVGEISDTLRSVFGEYQESVVI"
     gene            complement(2270..2524)
                     /locus_tag="VII37_07380"
     CDS             complement(2270..2524)
                     /locus_tag="VII37_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069592.1"
                     /translation="MSSAASLPGARTKLGGFRRLWRTLKQLFHEVIGALFAVLAFSWL
                     NAAIRAWSRDAAYWLVGIAAVLGALFLFFAVTSFRRSRKI"
     gene            2700..3062
                     /locus_tag="VII37_07385"
     CDS             2700..3062
                     /locus_tag="VII37_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069593.1"
                     /translation="MQLDDHLKNLLRELGHAINDTVSDSDRITGAIAGVRAHGYDIVL
                     KLDATIGLAKRDAASGETTSLTTQDRRFLESLRIQVDQDSFMEESEVNKKIPARLSMT
                     PQDVRFLKSLRIATDEME"
     gene            3161..3451
                     /locus_tag="VII37_07390"
     CDS             3161..3451
                     /locus_tag="VII37_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069594.1"
                     /translation="MLDTAVPIVVNAVKPKPTGLSKFEGYVMNANIAQITVRAKGNDM
                     AIQTFALSPQAAAKMQGIVDKGGYQYGDKVTLLYDPASLQVIKLKGKPSKAS"
     gene            3467..4276
                     /gene="amrB"
                     /locus_tag="VII37_07395"
     CDS             3467..4276
                     /gene="amrB"
                     /locus_tag="VII37_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY5069595.1"
                     /translation="MVRLPAVAGRFYPSDPAELSAQISEYTSTKGESASRNAKACLVP
                     HAGLMYSGHVAGAVFSSVRIPKKIVVLGVRHRPPGSPAAIISNGAWRTPLGDAQIDQE
                     LAEKLRVACPLLTEDAVAHSKEHSLEVEIPFLQVLAPDFRFVPIALGTAHFETMVNVG
                     QAIGEAVAAEKQEILLVTSSDLNHYEDDGTTRVKDRKAIEQLLKMDARGLYDVCRKEE
                     ISMCGLGPAVAMLTALQVLKASNAELIRYATSADRGGDTSAVVGYAGMVFW"
     gene            complement(4350..>5381)
                     /locus_tag="VII37_07400"
     CDS             complement(4350..>5381)
                     /locus_tag="VII37_07400"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY5069596.1"
                     /translation="ATANVSSAPQSDDFDRWVSGREDSVVTATNAAMHYSNSSSYVSG
                     FGDLYTYGGFYPIAGMGYGWQPYGVGLGWSPFDYGNWFYGPQFGWSFIGSQPWGWLPY
                     HYGGWFFQPGIGWLWTPTGFGGGNVGVKYHPVTATFVRSGSTVAMVPTHPLDSGSKTP
                     LNLAHIMPLTSASGAQPVSADGKWKVVKEVPAQALVSHATSAAVPGRSTRSFSGSGQS
                     NGSTIAYNAKEHRFVNSNVNAASAALTSPEMAAKGSATSTSPARSIATNQQVRAVPSA
                     SSRVVPSASARVAAPPPAARSYASARGGSFEGGSVARGARSQMATSSHETMSSAGRSS
                     SSSGGGRAH"
BASE COUNT         1287 a         1486 c         1492 g         1116 t
ORIGIN      
        1 aaccctcaat gcgcgggata tagcgcccgg cacggccttg aatcccgtaa gcgcccgctt
       61 tgtcgaaggg ctcgtcagtg gccaggtacc gcgaaatttc ttccggtgag attgcggaaa
      121 actgcacctg cgtggtttcc acaaaaccac ggcgctccgc atctggaagc cgaacaagca
      181 tgactccggt tacgaccgaa tgggttcgac cggagagcaa ctccagcgtt cgtcgggcgt
      241 cctcggcaga acgaggcttg ccaatgattt ggccgtccag caccaccacg gtgtcggcac
      301 cgatcacaat cgcggggcca accgaccgcg cggcgaccaa ttccactttt gcgcaagcca
      361 agcgatgtat ataatcgtta ggagtttcgc ccgggatcgg cgtttcatcc acagccgaag
      421 acatcaccgt aaaagtcatt cccgcatcac gaagaatttc ggcgcgacgg ggagaggcgg
      481 aagcaaggat cagtttcatg gcgggtggac cggcaagaat actacagagg ggaccgatgc
      541 gctgggaaga cgtagcgctg aacctgctgc tcaggagcgg gaattaaatt acaaccgact
      601 cctgatattc gccgaacacg cttcgcaaag tatctgaaat ttcgccgacc gtacaattat
      661 tttcaacggc gtcaagaatg gccggcaaaa gattctcagc tccagatgct ctgcgggcca
      721 actcagcgag aacggtctta acccgcgtgg catctcgttt caaccggaag gctttcaacc
      781 gctcaatctg atcgcgtgcg atttgttcct ccacccgcag aatagtaatg ggccgttctt
      841 cttccactct aaaatcgttc acgcccacta caatttgctc atttttctcg actgcgcgct
      901 gaaattcgta tgcggctttt tggatttcac cctgaataaa accggattcg atggcccgca
      961 gtgtcccatt taaagggccg cccatggcgt taattttgtc gatgtacatt tgcgcgccag
     1021 cttcaatttc gttggtaagt tgctcgatgg cgtaagagcc cgccaccgga tcgatgctgt
     1081 tggtgacccc cgtttcatgc gcgatgatct gttgcgtgcg cagcgcgatc aaagcggcat
     1141 cttccgtggg tagcgctagc gcttcgtcga gcgagttggt gtgcagcgac tggcacccgc
     1201 cgagaaccgc ggctaaagcc tgaatcgcta cgcgcacaat attattctcg ggttgctgcg
     1261 cggtgagcga agagcccgct gtctgcgcat gaaaacgcat cagccacgag cgcgggtctt
     1321 tggcgtggaa tcgctcacgc atgatgcgcg cccacaggcg gcgcgcggcg cgaaacttgg
     1381 cgatttcctc cagcaaatca ttgtgcgcgt tgaaaaagaa agacagctgc ggcgcgaaat
     1441 cgtccacgtt cagtccggca tcaagcgcgg cttgaacata ggccatgccg tctccgagcg
     1501 tgaatgccac ttcttgtatg gccgtggagc cagcctcgcg aatgtgatag ccggagatag
     1561 agatagtgtt ccacttggga agctgctcac tacaccacga aaaaatatct gtgacgatac
     1621 gcatggccga acgcaccgga tagatgtacg tcccgcgcgc gatatattcc ttgaggatat
     1681 cgttctgtac cgtgcctgaa agcttcgcaa ggctggcgcc ctgctttttt gcgaccgcca
     1741 catacaggca aagcagaatc gcagcggtgg agttgatggt catggaagtg gaaacttttt
     1801 ctagcgggat acggtcaaaa agtatttcca tgtcttcgat ggaatcgatg gccacgccac
     1861 ccttaccaac ttcgccatga gccagaggat gatcggagtc cattccgatt tgcgtgggca
     1921 agtcgaaggc tacggaaagt cccacctgac ctttgccgag gagatagcga taacgctcgt
     1981 tggattccgc tgcgctcgcg aaccctgcat actggcgcat ggtccaaaaa cggccgcgat
     2041 acatcgtcgg ataaatccca cgtgtatagg gaaattctcc ggggtagccg atagtctctt
     2101 cacgattccg ggaagctaaa tcctcagcgg tgtataaagc ctccagcggc aagcctgaca
     2161 aggtagtgaa tttttcttta cgatccgagc ggcgcgcagc agaactaacg ggtttggaat
     2221 tagaatcagc cataaattta gtcgagtttg tgcaactctc tcaaggccgc taaattttcc
     2281 gagagcgccg gaatgacgta acggcaaaaa ataaaaatag cgcgccaagc acagccgcaa
     2341 tccccaccag ccagtaggcg gcgtcgcggg accaggcgcg aatcgctgcg ttcagccagg
     2401 agaatgccaa taccgcgaaa agcgcgccga tcacctcatg gaagagctgc ttgagggtgc
     2461 gccacaagcg ccggaagccg ccgagtttgg tgcgtgcgcc tggcaagctg gcagcagaag
     2521 acatgctggt atcttaacgt aaaagcattc tcgccctccg gttctacggg taaagatgta
     2581 gaagcgggtc gccaaggctg cccggaggac ctgtgaacga attggagaca acaacttgaa
     2641 gctcgcttgt atccaatcaa acgtgggcct aaaatgtcga agagtagggg agaaccgcct
     2701 tgcaactaga cgatcacctg aagaacttgc tccgtgaatt gggacacgcg atcaacgaca
     2761 cagtgtccga ctctgaccgc atcaccggcg cgattgccgg agtgcgcgcg cacggctacg
     2821 acatcgtgct gaagctggat gccaccatcg gcctcgccaa gcgcgatgct gcgtctggag
     2881 agacgacttc gttgaccact caggatcgcc gcttcctgga atcgctgcgc attcaggtag
     2941 atcaggattc attcatggaa gagagtgaag tgaacaagaa aatccctgcg cgactctcga
     3001 tgacgccgca ggatgtgcga ttcctgaagt ctttgcggat cgccacggat gaaatggaat
     3061 gaagtgttta cttaaagcgg cgggattcgt attcgtccta acagggatga taatgtgccc
     3121 gcgcgctcag gcgcagagcc ccgcgacagt tgtaccaatc gtgttggaca ccgccgtgcc
     3181 tatcgtagtc aacgcggtaa agcccaagcc aacaggatta agcaaatttg aaggttacgt
     3241 catgaacgcg aacatcgcgc aaatcactgt gcgcgccaag ggcaatgaca tggcgataca
     3301 gaccttcgcg ctctcgccgc aagcagccgc caagatgcag ggcattgtcg acaagggcgg
     3361 atatcagtac ggagacaaag ttacgttgtt gtacgatccg gccagtttgc aagtcatcaa
     3421 gctcaaaggg aaaccctcca aagcctctta aattctgcaa aagcaaatgg ttcgtctccc
     3481 cgccgttgcc ggtcgttttt atccttccga tcccgctgaa ttgtccgctc agattagcga
     3541 gtacacctca acaaaaggcg aatctgcttc tcgcaatgcg aaggcctgtc tggttccgca
     3601 cgccggcttg atgtattcgg gccatgtggc aggtgccgtt ttttcgagcg ttcgcatacc
     3661 aaaaaagatt gtggtactcg gcgtccggca tcgtcctcct ggttcgcccg cggctattat
     3721 ctccaacggg gcgtggcgca ctccgctggg tgacgcgcaa atcgaccagg agttggccga
     3781 gaaactccga gtagcttgcc cgctgctaac cgaagacgcc gtggcccaca gcaaagaaca
     3841 ctccttggaa gtcgaaattc cgtttctcca agtgctcgct ccagattttc gtttcgtgcc
     3901 aatcgcgctg ggaacggcgc actttgaaac tatggtgaat gtgggacaag caataggtga
     3961 agcggtcgcc gcggaaaagc aggaaattct attggtcact agctccgacc tgaatcatta
     4021 cgaggatgat ggtacgaccc gtgtgaaaga ccgcaaagcc attgagcaat tgttgaaaat
     4081 ggatgcgcgc ggtttatacg atgtctgccg caaagaagaa atttccatgt gcggtctggg
     4141 cccggcggtc gcgatgctca cggctttgca agtattgaag gccagcaatg cggaattgat
     4201 tcgctatgct acgtcggctg atcggggagg agatacttcc gctgttgtgg gatatgcggg
     4261 aatggttttc tggtgattaa tcgcggcacg aacacaaaaa aaagacggcc gcaaatccag
     4321 ccgtctttcg tttcaagtat ttcgccaaac tagtgcgcgc gaccgcctcc cgaactggat
     4381 gagctgcggc cagccgacga cattgtctca tgactagacg ttgccatttg tgaccttgcg
     4441 ccacgtgcaa ccgagccacc ttcaaaagat ccaccgcggg cacttgcgta ggagcgcgca
     4501 gcgggagggg gagccgctac gcgggctgag gccgaaggta ccaccctgga ggatgccgaa
     4561 ggcacggcgc gaacttgctg gtttgtagca atggagcggg cgggcgaagt gctggttgca
     4621 gagccctttg ctgccatctc cggcgaagtc aacgccgccg aagccgcatt cacgttgcta
     4681 tttacaaagc gatgttcctt ggcattgtaa gcaatggttg aaccattgct ctgaccggaa
     4741 ccactgaagc tgcgtgtgga gcgccccgga accgccgcag acgtcgcgtg cgaaaccaac
     4801 gcctgagcgg ggacttcctt tacaactttc cacttgccat cagccgaaac cggctgggca
     4861 ccgctcgcag aagtcagcgg cataatatgt gccaaattta acggcgtctt gctgccgcta
     4921 tccagtggat gagtgggaac cattgccacg gtgctgccag aacgcacaaa cgtcgccgta
     4981 acaggatggt atttaacacc tacgtttccg cctccgaatc cagtcggagt ccacagccaa
     5041 ccgataccag gttggaaaaa ccagccacca taatgatacg gcagccatcc ccacggctgg
     5101 cttccgataa aagaccatcc aaattgcggc ccataaaacc agttgccgta atcgaacggt
     5161 gaccagccca gcccgactcc ataaggttgc catccgtaac ccatgccggc gatgggataa
     5221 aaaccgccgt aggtgtacaa atcgccaaat ccggatacgt aggaagaaga atttgaatag
     5281 tgcatcgccg cattagtggc ggtgacaacg ctatcttcgc ggcctgatac ccaacgatca
     5341 aaatcgtcgc tctgcggagc actgctgaca ttcgcggtag c
//
LOCUS       DATCBB010000671         4193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_442822, whole genome shotgun sequence.
ACCESSION   DATCBB010000671 DATCBB010000000
VERSION     DATCBB010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442822"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1434)
                     /gene="mqnE"
                     /locus_tag="VII37_07405"
     CDS             complement(277..1434)
                     /gene="mqnE"
                     /locus_tag="VII37_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEY5069597.1"
                     /translation="MAELLITDQHLKPIAAKVLAGERLSQDDGVALYRSPDLLAVGWL
                     ANHVREKRHGNVTYFNVNRHINPTNVCVAHCKLCAFGRDPNAPGAYTFALEEIYQRAE
                     QGVREGATEFHMVGGLHPDLPFEYFLDMIRGLKQRCPTVHLKAFTMVEVGYFARIAKL
                     SVKETLQKMKDAGVDSLPGGGAEIFPPRVRKLICDHKVSGQMWLSIARQAHELGFHSN
                     ATMLYGHVETEEERVDHLIKLRDLQDQTHGFVTFIPLAFHPDNTALSHIPKPTGYADL
                     RNIAVSRLLLDNFAHIKAYWIMLSPAIAQIALRFGANDLDGTVVEEKIYHDAGAVTSE
                     FTSRADLERLIRAAGRVPVERDTLYNPVDRSKMPPIPPPTRPDLVSLTLNV"
     gene            complement(1514..2428)
                     /locus_tag="VII37_07410"
     CDS             complement(1514..2428)
                     /locus_tag="VII37_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEY5069598.1"
                     /translation="MATELTPGIVPGVKRPNRIKTVLEMIKFEHSVFALPFALTGALL
                     GARATHQGWPTLRQLTWVVIAMVAARSAAMTVNRIVDLRYDRENPRTRQRALVTGSLS
                     VPFAWTFTLIAVAIFFLAAWQLNLLALELAPLAVAILFFYSFTKRFTTWSHLFLGFAL
                     GISPAAAWIAITGGFDPRTLILCAAVTLWVGGFDVLYACQDIEYDRSAGLFSIPQRFG
                     IANALLIARIMHVGVVALLIWLAASFALPWPAWLGIAVVAALLGYEHSLVKADDLSKL
                     DAAFFAMNGYISMSFLLFWGAAVALWRV"
     gene            2608..3000
                     /locus_tag="VII37_07415"
     CDS             2608..3000
                     /locus_tag="VII37_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5069599.1"
                     /translation="MASAMASAAPLETVVEFIKRINAGNVDKLCELMTEDHVFQDALG
                     KRFIGRETLRGGWKMYYEMVSDYKVRGEQFFVDKNVVAVFGTASGTSKRDGKFSNEGF
                     WEIPAAWKAIVRDGQIAEWCVYAESSRA"
     gene            3052..3819
                     /gene="larB"
                     /locus_tag="VII37_07420"
     CDS             3052..3819
                     /gene="larB"
                     /locus_tag="VII37_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEY5069600.1"
                     /translation="MTESDLLQLLDEVRSGALAPRKAMERLKLLPFEDLDFAKVDHHR
                     TLRQGFAEVVFGKGKTTAQVAEIVKALLGRKPGRQNVLVTRADSKMFAAVKRAAGKKN
                     GAVKFHQLSGAITIERNQEISGKGTIVVVSAGTSDIPVAEEAVLTARLMGNRVEHLYD
                     VGVAGIHRLLKHSEGLMRARVIICVAGMEGALPSVVGGLVAVPVIAVPTSVGYGASFG
                     GVAALLGMLNSCASNVTVVNIDNGFGAARVASCINRL"
     gene            complement(3912..>4193)
                     /locus_tag="VII37_07425"
     CDS             complement(3912..>4193)
                     /locus_tag="VII37_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069601.1"
                     /translation="LALAQHMTEPRGTLVLKSTFHGAAPVETWPLVVKEITLIGSRCG
                     PFVKAIALLRSGKVDPRPLITRTFPLSQAPAAIKYAQQPGVMKVLLKPE"
BASE COUNT          942 a          998 c         1379 g          874 t
ORIGIN      
        1 agccgatgcg gttggcgggc acctggaaac aatattcgaa gaaggcgatg ccccaactgg
       61 cgagaatcac tttccagagc gcctcgttgc gatacttcag gtgaccgtac caggcgaagg
      121 tcatgaagat attcgaaatg gtgagcagga gaattgttcg cattgtgcag attttcctcg
      181 acgtggatat taatgtgccc ggagcttatg gcaaatacag atttctacgc ggttgcagga
      241 gcggggcaaa gcggatcgaa gaagcttgcc tggaacttaa acgttgaggg tgagggagac
      301 gaggtctggg cgagtgggcg gaggaatcgg aggcattttg gagcgatcca cgggattgta
      361 gagggtgtcg cgctctacgg ggacgcgacc ggcggcgcga atgaggcgtt ccagatccgc
      421 gcgcgacgta aattcagagg tgaccgcgcc ggcatcgtga taaatctttt cttcgacgac
      481 cgtgccgtcg agatcgttgg cgccaaagcg cagcgcgatc tgcgcaatcg cgggagacag
      541 catgatccaa taggccttga tgtgcgcgaa attatcgagg agcaagcgcg ataccgcgat
      601 gttgcgcagg tcggcgtaac cggtgggttt gggaatgtgc gaaagcgcgg tgttgtccgg
      661 atgaaatgcc aaaggaataa aagtgacgaa gccgtgagtc tgatcctgca ggtcgcgcag
      721 cttgatcagg tggtcgacgc gttcttcttc ggtttccacg tggccgtaga gcatggtggc
      781 gttggaatgg aagccgagtt cgtgggcctg gcgcgcgatg cttagccaca tctggccgct
      841 cactttgtgg tcgcagatca gcttgcgtac gcgcgggggg aaaatttccg cgccgccgcc
      901 cggcaaggaa tcgacgccgg cgtctttcat tttttgcagc gtttccttaa cggagagctt
      961 ggcaatgcgc gcgaagtagc cgacttccac catggtgaag gctttgagat gcacggtagg
     1021 gcagcgctgc ttcagaccgc gaatcatatc caggaaatat tcaaagggca gatcgggatg
     1081 caggccgccg accatgtgga attcggtagc gccttcgcgt acgccttgtt cggcgcgctg
     1141 gtagatttct tcgagggcga aggtgtaggc gccgggggcg ttggggtcgc gaccgaatgc
     1201 gcacagtttg caatgcgcga cacacacgtt ggtgggattg atgtgtcggt tgacgttgaa
     1261 ataggtgacg ttcccatggc gtttttcgcg tacgtgattg gcgagccagc ctacggcgag
     1321 gagatcgggc gagcgataca gcgcgacgcc gtcgtcctgc gacaggcgct cgccagctaa
     1381 aactttagcg gcgatgggtt tcaaatgctg gtcggtgatg agtaactctg ccacgtctat
     1441 atcttatcct ttgccggaat acctggcgat tgcagcagga atttcaaacg tttcaatatc
     1501 ttcaaacgtg attttatacg cgccagaggg ccactgctgc gccccagaat aaaagaaacg
     1561 acatactaat ataaccgttc attgcgaaaa acgcagcgtc gagcttgctc aggtcgtcgg
     1621 cttttaccag cgagtgttcg tagccgagca atgcggcgac tactgcaatg cccagccacg
     1681 ccggccatgg caacgcgaag ctggcggcga gccatatgag cagcgcgacc acgccgacat
     1741 gcataattcg cgcaatgagc agcgcgttag cgattccgaa acgttgcggt atggagaaaa
     1801 gcccggcgct gcgatcgtac tcgatgtcct ggcacgcgta taaaacgtcg aagcctccga
     1861 cccacagcgt gactgcggcg cagagaatca gcgttcgcgg atcgaagccg ccagtgatgg
     1921 cgatccatgc ggctgcggga gaaatgccta acgcgaagcc gagaaaaaga tgcgaccagg
     1981 tagtgaaacg cttggtgaag gaatagaaaa agagaatggc cacggcgagc ggtgcgagtt
     2041 cgagcgccaa aagattcagc tgccacgcgg cgagaaagaa aatagccacc gcgatcagtg
     2101 tgaaggtcca ggcgaaaggc acggagagcg atccggtgac gagggcgcgc tggcgggtgc
     2161 gcggattttc tcgatcatag cgcaggtcca cgatgcgatt gacggtcatg gcagcggatc
     2221 gggctgccac catggcgatt accacccatg ttagttgacg cagcgtcggc caaccttgat
     2281 gagtggcacg cgcgccgagc aacgctcccg tcagggcgaa cggcagggca aacacggaat
     2341 gctcgaactt gatcatttcg agcacggttt tgatgcggtt ggggcgtttc acgccaggaa
     2401 cgatacccgg tgttaattcc gtcgccaaat caagcctctg cttttttgct gccgtcgtgg
     2461 aagtgcttcg ccacgactaa atcatgtgca atttgtggat agattcagtg tcgcaacgcg
     2521 atgaaggcaa gtcaagattg ccaggacgaa gagtgcgatt tggtgcgacg tcgttatact
     2581 ctagattccc ggcggaagga gagcccgatg gcgagtgcga tggcgagcgc ggcaccactg
     2641 gaaacggtag tggagttcat taaacggatc aacgcgggaa atgtagacaa gctgtgcgag
     2701 ctgatgaccg aagatcacgt gttccaggac gcgctgggaa agcggttcat tgggcgggag
     2761 actttgcgag gcggctggaa gatgtattac gaaatggttt cggattacaa ggtgcgcggt
     2821 gagcagtttt ttgtggataa gaatgttgtg gcggtgtttg gcaccgctag cggcacgagt
     2881 aagcgcgacg gcaaattttc caacgaaggc ttctgggaga taccggcggc ctggaaagct
     2941 atcgtgcgag acgggcagat tgccgagtgg tgcgtttacg cggaatcctc acgcgcgtga
     3001 aggcgctcgt tgagcgcccg ataaaaagaa aagataaaac gaaacatcct tatgaccgaa
     3061 tctgacctgc tgcaattgct tgatgaagtg cgctccgggg cgctggctcc caggaaagcc
     3121 atggagcggc tgaaacttct accgtttgaa gatttggatt ttgccaaggt ggatcatcac
     3181 cgcacattgc gccagggatt cgcggaagtt gtttttggga aaggtaaaac cacggcacag
     3241 gtggcggaaa tagtaaaagc gttgctgggg cgaaagcccg ggcgacaaaa tgttctggtg
     3301 acgcgcgcgg attcgaagat gtttgccgcg gtgaaacgag ctgctggtaa gaaaaatgga
     3361 gcggtgaagt ttcatcagct gtccggcgcg atcactatcg aacgcaacca ggagatttct
     3421 ggtaagggaa ccattgtcgt ggtttctgcg ggcaccagcg acattccggt ggccgaagag
     3481 gcggtgctca ccgcgcgctt gatgggtaat cgcgtggagc atctgtatga cgtgggcgtg
     3541 gcggggattc accggctgtt gaagcacagc gaaggattga tgcgcgcgcg cgtgattatt
     3601 tgcgtagcgg gaatggaagg ggcgctgccg agcgtagtgg gaggtctggt ggcggtcccg
     3661 gtcatcgccg tgccgacgag cgttggctat ggtgcgagct tcggtggtgt ggcggcgttg
     3721 ttggggatgt tgaatagttg cgcgtcaaac gtgacggtag tcaacatcga taacggtttt
     3781 ggcgcggcgc gcgtggcgag ctgcattaac cgcctgtgag ttgagctgga tttgcgaaaa
     3841 actcgttgcc ctcaaaaaaa gcgtggcaca accgccttct agttttgtct aaatagaaac
     3901 ttcccagaaa atcattcagg ttttagcagg accttcatta cgcccggttg ctgcgcgtat
     3961 ttaattgcgg cgggggcttg gcttagtggg aatgtgcggg tgatcagcgg gcgcgggtcc
     4021 acctttccag agcgcaacag tgcgatggct ttaacgaacg ggccgcagcg cgatccaatc
     4081 agggtgattt cttttactac tagcggccag gtttctaccg gggcggcgcc gtgaaatgtg
     4141 gacttcagga ccagcgtacc gcggggctcg gtcatgtgtt gcgcgagagc cag
//
LOCUS       DATCBB010000672         1659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_183274, whole genome shotgun sequence.
ACCESSION   DATCBB010000672 DATCBB010000000
VERSION     DATCBB010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183274"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VII37_07430"
     CDS             complement(<1..678)
                     /locus_tag="VII37_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEY5069602.1"
                     /translation="MVIVMQEGATDAQIQHVIDRLVGSGFNVHRSTGESHTVLGAVGV
                     HRDFDHRDFELLDGVREVMRVTQPFKLASRQFNPQGTIINLGRGVTIGGNEVVVAAGP
                     CSVESREQIHIVAESVAKSGAKILRAGAFKPRTSPYAFQGMGEKGLELMREAAEKFGL
                     FTVSEVMDASQIQMMIGYADILQVGARNMQNYHLLRALGEIRKPILLKRGMSATMEEL
                     LLSAEYIL"
     gene            complement(737..1027)
                     /gene="pheA"
                     /locus_tag="VII37_07435"
     CDS             complement(737..1027)
                     /gene="pheA"
                     /locus_tag="VII37_07435"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY5069603.1"
                     /translation="MNLQDWRSRIDEIDRKLVELLNERSRCALEIGKLKQAANLPLYQ
                     PEREKEVLQNAEQSNPGPLTDAAIRRLFERIIDEARSAERAAMEPEAKSSKK"
     gene            complement(1024..>1659)
                     /gene="trpA"
                     /locus_tag="VII37_07440"
     CDS             complement(1024..>1659)
                     /gene="trpA"
                     /locus_tag="VII37_07440"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY5069604.1"
                     /translation="IQRASERALKSGASLPKILELVRRIRKSSQIPLVLFSYYNPILQ
                     MGLEAFAAAASQAGVDGVLATDLSPEESVEYRRILATHHLDTIFLCAPTSTDERLASI
                     AKCSSGFLYLVSRTGVTGAKDTLPDDLPALLRRVRNFTTLPIAVGFGISLPGHITLLG
                     GLADAAVVGSSLVAEIEHAKTSQQAAKALRERIGILKQAARHGLSKREVAP"
BASE COUNT          346 a          479 c          443 g          391 t
ORIGIN      
        1 gaggatgtac tcggcgctca ataacagctc ttccatggtt gcggacattc cgcgcttgag
       61 caagatgggc ttgcgtattt cacccaaggc gcgcagaaga tgatagttct gcatgttgcg
      121 cgcgccgacc tgcaaaatat ccgcgtagcc gatcatcatc tgaatctgcg aagcatccat
      181 gacctcgctt acggtgaaga ggccgaactt ttcagcggcc tcacgcatca gttccaggcc
      241 cttttcgccc atgccttgga aggcgtacgg tgatgtgcgg ggcttgaatg ctccggcgcg
      301 caaaattttg gctccggatt tggccacact ttccgcgaca atgtgaatct gctcgcgcga
      361 ttccacggaa cacgggcccg ccgcgacgac cacttcatta ccgccaatgg ttacaccgcg
      421 acccagattg ataatcgttc cttggggatt aaattggcgg ctggccagct tgaagggctg
      481 cgtgacgcgc ataacctcgc gcacgccatc gagcaattcg aaatcgcggt gatcgaaatc
      541 gcgatgcacg ccaacggcgc caagcacggt gtgcgattct ccggtggagc gatgcacgtt
      601 aaatccgctt cccaccagcc ggtcgatcac atgttgaatc tgggcgtctg tagcgccctc
      661 ttgcatgaca attaccatga gcgcgtttta ttcccctctt cgattggcat tggtctctcg
      721 ttcttcttga cccaacttat tttttggaac ttttcgcttc cggctccatg gccgcgcgtt
      781 ccgccgaccg cgcttcgtcg ataatgcgct cgaatagacg gcgaatcgcg gcatctgtta
      841 gcggacccgg attcgattgc tcggcgtttt gcaacacttc cttctcccgc tccggctgat
      901 acagcggcaa atttgctgct tgcttcagct tgccgatctc cagtgcgcag cgcgagcgct
      961 cattgagcaa ttccaccagc ttgcgatcga tctcatcgat gcggcttcgc cagtcctgaa
     1021 gattcacggc gctacctcgc gcttggaaag cccgtggcgc gccgcttgtt tcaagatacc
     1081 gatccgttcg cgaagagcct ttgcggcttg ttgcgacgtt tttgcgtgtt caatttcagc
     1141 aaccaaagag gaccccacta ctgccgcatc tgcaagcccg cctaacaaag taatgtgacc
     1201 cggaagcgag attccgaagc ctacggcaat aggcagcgtc gtaaaatttc taactcgccg
     1261 tagcagtgcc ggcaaatcat cgggcaaagt atctttcgct ccagtaacac ccgtacgcga
     1321 tacgaggtac aaaaagccgc tcgagcattt tgcaatgctt gctagccgct cgtccgttga
     1381 agttggcgcg cacagaaaaa ttgtgtccaa gtgatgcgtc gcgagaatgc gccggtactc
     1441 cacggattct tccggtgaaa gatcggtggc gagtacaccg tcgactcctg cttgggaagc
     1501 cgcagcggca aatgcttcca gccccatttg cagaatggga ttgtaatagc taaagagcac
     1561 caggggaatt tgtgaagatt tccggatgcg gcgcaccagt tccagaattt ttggcaagct
     1621 ggcgccggat ttcaatgcgc gttcggatgc gcgttggat
//
LOCUS       DATCBB010000673         1199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_442861, whole genome shotgun sequence.
ACCESSION   DATCBB010000673 DATCBB010000000
VERSION     DATCBB010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442861"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VII37_07445"
     CDS             complement(<1..594)
                     /locus_tag="VII37_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5069605.1"
                     /translation="METPLTPLDFARRARKLYGGREAVVDGDLRLTYAQFFDRCDRWS
                     ATLQKLGVAQGDRIAYIAPNTHAQLESFYAVPQIGAVLVPVNYRLTPDDFAYLINHSG
                     ARVVCAHSDYLAAVDGIRSQLPRVEHFVALEGSPAGWLGYESLLAEGASDFTRPTIRE
                     SDFLTINYTSGTTSRPKGVMITHRNAYMNTVGTLVHEH"
     gene            complement(701..>1199)
                     /locus_tag="VII37_07450"
     CDS             complement(701..>1199)
                     /locus_tag="VII37_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069606.1"
                     /translation="WLYSRIAEESIKQNADQTQAREVLDEVVAAAAKAPKTPVTARAL
                     LSVAYLYTKIDMNQAIAVMGEAVKCINQIENPDFSRPGVIRRIEGKGFARYASFVTPG
                     FSPESAFREIAKVDFDGMLNQAANFSDKSLRALTTLAVVEQCLKDLTAAPKPKQSKPA
                     PAKPE"
BASE COUNT          260 a          343 c          335 g          261 t
ORIGIN      
        1 gtgctcgtga accagcgtgc ccaccgtatt catgtaagcg ttgcgatgcg tgatcattac
       61 gcccttgggc cgcgaagtag tgccactggt gtagttgatc gtgagaaaat cactttcccg
      121 gatcgttggc cgagtaaagt cgctcgcacc ctcagccagc aacgactcgt aaccaagcca
      181 gccggcaggt gaaccctcaa gcgcaacgaa gtgctcaacc cgcggcagtt gcgatcgaat
      241 accatcgacg gccgcgagat aatccgaatg agcacaaacc actcgcgccc cactgtgatt
      301 gatcagataa gcgaagtcgt cgggcgtcag acgatagttg accggcacca gcaccgcgcc
      361 aatctgtggc actgcataga acgactcgag ctgggcgtgc gtgttgggtg cgatgtaggc
      421 gatgcgatca ccttgagcca ccccgagttt ctgcaaagta gccgaccagc gatcgcaccg
      481 gtcaaagaac tgcgcatacg tcagacgcag atcaccatcc acgacagctt cgcggccgcc
      541 gtacagcttt cgcgcgcggc gcgcaaagtc caggggagtt agcggagttt ccattcgcct
      601 tgtctggaat gatttttgtg cctgagaacg ggcgagactg tatcaacgcg caactggcgg
      661 acgcaagctt catatgatcg caacggttta cctgcgaagt tcattccggc tttgccggcg
      721 ccggtttact ttgctttggt tttggcgccg cggtcaggtc tttcaggcat tgttcgacaa
      781 ccgcaagcgt ggtgagcgcg cgcagcgact tatcggaaaa attggcagcc tgattcagca
      841 tgccgtcgaa atcaactttg gcaatttcgc gaaaggcgct ttcagggctg aagccgggag
      901 ttacgaatga cgcgtacctg gcaaacccct tgccttcaat cctcctgatg acgcccggcc
      961 ggctgaaatc cgggttttcg atttggttga tgcatttaac ggcctcgccc atcacagcaa
     1021 tagcctgatt catatcaatt tttgtataaa gatatgccac gctcaacaaa gcgcgagcag
     1081 tgaccggagt ctttggcgcc ttcgccgcgg cggcaacgac ttcgtcgagc acttcacgcg
     1141 cctgggtctg gtctgcgttc tgcttgatcg attcttcggc aatgcgcgag tagagccac
//
LOCUS       DATCBB010000674         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_54699, whole genome shotgun sequence.
ACCESSION   DATCBB010000674 DATCBB010000000
VERSION     DATCBB010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54699"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VII37_07455"
     CDS             complement(<1..741)
                     /locus_tag="VII37_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY5069607.1"
                     /translation="MNPIRSILLAASQNAWLRDHAARHTFVRRAVSRFMPGEELSDAL
                     SAAQRLQSKNIGSIFTRLGENITDEAEARSVAEHYLEVLRSVREKQLDVEISVKLTQL
                     GLDLSSELCYANLKSIIEQEDSSGVVWIDMEASNYVDATLQMYLRALQAFPNAGVCLQ
                     AYLHRTRDDLASLLPLRASIRLVKGAYSEPPEIAFPEKSRVDENYFLLAKEMLLAKKS
                     EPKIRAAFGTHDMVLLRRISEFAAASGLP"
     gene            complement(763..1497)
                     /locus_tag="VII37_07460"
     CDS             complement(763..1497)
                     /locus_tag="VII37_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY5069608.1"
                     /translation="MTMPSPLLGEFLGTMIMILLGDGVVAAVLLKRSKAEGSGWMVIT
                     AGWAFAVMAGVFVALACGGEANLNPAVTLGLAVRTGIYTKFLPFFVVQLLGGIAGAAL
                     VWLQYLPHWRETPDAPAKLACFCTSPAIRSFLPNLLSEIIATFVLIFVIGAIYSRLVF
                     ANGPGPGFLAVYLVSSLVWGVGLSLGGSTGYAVNPARDLGPRIAHAILPITGKGGSDW
                     SYAPVPILGPLVGGALAGLSLRLLNI"
     gene            complement(1522..>2847)
                     /gene="glpK"
                     /locus_tag="VII37_07465"
     CDS             complement(1522..>2847)
                     /gene="glpK"
                     /locus_tag="VII37_07465"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY5069609.1"
                     /translation="AEGLAQRSLQPDDLAAIGITNQRETTVVWDRKTGEPIANAIVWQ
                     DTRVSEDVARFAAKGGQDRFRARTGLPLSTYFSALKIRWLLENVSGARAKAAAGDLLF
                     GNIDTFLVWNLTGGVAGGVHVTDVTNASRTQLFNLQALDWDPELLSAFDIPRAMLPQV
                     RSSSEIYGEAAIHEIKGVPIAGILGDQQAALVGQACFQPGEVKNTYGTGCFLLMNTGE
                     RMVPSKCGLLTTVAYKFGSHAAHYALEGSVAIAGALVQWLRDNLGLIQRSEDVETLAR
                     TVNDNGGVYFVPAFSGLYAPHWKENARGVIAGLTRYANKGHLARAVLEATAYQTREVV
                     EAMEQDAGISLANLRVDGGMVVDELLMQFQADILRREVLRPVIQETTALGAAYAAGLA
                     VKFFSGLEELRANWAVARSWKPRLDEATREQLYRQWKKAVVRSLDWADS"
BASE COUNT          630 a          851 c          780 g          586 t
ORIGIN      
        1 cggaaggccg ctggctgcgg caaattctga aatgcgccgc aacaatacca tgtcgtgcgt
       61 tccgaacgcg gcgcggattt tcggctcaga cttttttgcg aggagcattt ctttggcgag
      121 cagaaaataa ttttcgtcca cgcgcgactt ttccgggaag gcgatctcag gaggctcgct
      181 gtatgcccct tttaccaagc gaatggaggc tcgcaagggc agcaggctcg ccagatcgtc
      241 tctggtgcga tgcaagtaag cctgcaggca cacaccggcg ttgggaaacg cctgaagcgc
      301 gcgaaggtac atttgcagcg tggcgtccac gtagttgctg gcttccatgt cgatccacac
      361 cacgccgctt gaatcttcct gttcaataat acttttcaga ttggcgtaac aaagctcgga
      421 agacaggtcc agccccagct gcgtgagttt tacggatatt tccacgtcaa gctgcttttc
      481 tcggacgctg cgcaaaactt ccaggtaatg ctccgcgacg ctgcgcgctt cggcttcgtc
      541 ggtgatgttc tctcccaggc gggtgaagat cgatccgata tttttgctct gcaaacgctg
      601 cgcggcggac agcgcgtcgc tgagctcttc gcccggcatg aatcgtgaca cggcgcggcg
      661 gacgaaggtg tgtctggcgg cgtgatcgcg cagccaggca ttctgcgaag cagcaagcaa
      721 gatggatcgg ataggattca tttcacaatt tcaaatgata tttcaaatat tgagaagccg
      781 cagcgagagt cccgctaacg ccccaccgac tagcggcccg aggatgggca ccggagcgta
      841 gctccaatcg gacccacctt tgccggtgat cggcaaaatg gcatgcgcga tgcgcggccc
      901 caaatcacgc gcgggattga cggcatagcc tgtggagccg ccgagcgaaa ggccgactcc
      961 ccagaccagg ctggaaacca ggtacaccgc caaaaatccc ggccccggtc cgttggcaaa
     1021 aaccaagcgc gaataaatcg cgccgatcac aaatatcaaa acaaatgtgg caatgatttc
     1081 gctaagcagg tttggtagaa agctgcggat cgcgggagac gtgcagaagc acgccagttt
     1141 tgcgggagca tccggcgttt cgcgccaatg cggaagatat tgcagccaga ccagcgcagc
     1201 gcctgcgatt ccgccaagta gttgcaccac aaaaaaaggc aggaacttag tatagattcc
     1261 tgtgcgtacc gccagaccca aggttaccgc gggattcaag ttggcttcgc cgccgcaagc
     1321 gagagccacg aatactccgg ccattaccgc aaaagcccaa cccgcggtga tgaccatcca
     1381 gccgctgcct tctgctttcg aacgcttcag gagcaccgcg gcaaccacgc cgtcacccag
     1441 caaaatcata atcatggtgc caagaaattc gccgagcaga ggcgatggca tggtcatcaa
     1501 atctccttat gcgttgtaaa tctaggaatc agcccagtcc aatgagcgaa ctacggcttt
     1561 cttccattgc cggtaaagtt gttctcgggt cgcttcgtcg agtctaggct tccatgaacg
     1621 cgccacggcc cagttggcgc gcaactcttc cagcccggaa aaaaacttta ccgctaatcc
     1681 tgcggcgtaa gcggcaccca gtgcggtcgt ttcttgaatc accggacgca agacctcgcg
     1741 acgcaaaata tcagcctgga actgcatcaa caattcatcg acgaccatgc cgccatccac
     1801 ccgcagattg gcgagcgaaa taccggcatc ctgttccatg gcttccacca cttcgcgggt
     1861 ttgatacgcg gtggcttcca gcacggcgcg cgccaggtga cctttgttgg cgtaacgcgt
     1921 gaggccggca atgactcccc gcgcgttctc tttccagtgc ggcgcgtaca atcccgaaaa
     1981 agccggcaca aaataaacgc cgccattgtc gttgactgtg cgcgccaacg tttccacatc
     2041 ttcgctgcgc tggattaaac cgagattgtc gcgcagccat tgcaccagcg cgcccgcgat
     2101 ggctacgctg ccttccagtg cgtaatgggc agcgtgacta ccaaatttat acgctacggt
     2161 agtgagcagg ccgcatttgc tcggcaccat tcgttccccg gtgttcatta aaagaaaaca
     2221 accagtgccg taagtatttt tcacttcgcc gggttggaaa caggcttggc caaccaacgc
     2281 cgcttgctgg tcgccaagaa tgcctgcgat tggcacgcct ttgatttcgt ggatcgcggc
     2341 ttcgccataa atttcgctgc tggaccgaac ttgcggcagc atcgcgcgag gaatatcaaa
     2401 cgccgaaagc agctcgggat cccagtccag cgcttgcaaa ttgaaaagtt gcgtgcggct
     2461 ggcgttcgtt acatccgtga cgtgcactcc gcctgcaacg ccgccagtaa gattccagac
     2521 caagaaggtg tctatgttgc cgaaaagcaa gtcgccggca gccgctttgg cgcgcgcgcc
     2581 cgagacattt tccagcagcc acctgatttt cagcgcgctg aaatatgtgc ttaagggcaa
     2641 acccgttcgc gcgcgaaatc gatcctgtcc gcccttcgcc gcgaatcgcg ccacatcttc
     2701 ggagacacgc gtgtcttgcc agacaatcgc gttggcaatg ggctcgccgg ttttgcggtc
     2761 ccacaccacc gtcgtttcgc gctgattggt tatgccgatg gccgccaggt cgtctggttg
     2821 caggctgcgc tgcgccagtc cttccgc
//
LOCUS       DATCBB010000675         4961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_216780, whole genome shotgun sequence.
ACCESSION   DATCBB010000675 DATCBB010000000
VERSION     DATCBB010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216780"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VII37_07470"
     CDS             complement(<1..819)
                     /locus_tag="VII37_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5069610.1"
                     /translation="MKLTSRQQHALQLICDTFAPAADGWPSASELGVPQATADAMDFN
                     PRRGDHKQFLQLLNFWDSQFHSVISIKRASRFSTLSEEARVRVLLSWAESSLGRRRAA
                     FQALRKAVSYLYVMLPQLQGGRTPVWGKIGYPGPPNLANRASSSRLLTTLVPQQDTKL
                     SCEICIIGSGAGGGTAAAVLAAAGKDVIILEAGGYYDDADFDGAELAGYQRLYLEGGS
                     AATADHSVGLLAGECLGGGTVVNYTTSFRTPDDVRAEWAASGVPWFTRNEYTRSI"
     gene            complement(911..1693)
                     /locus_tag="VII37_07475"
     CDS             complement(911..1693)
                     /locus_tag="VII37_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069611.1"
                     /translation="MRPEQNPDTMLPEQRVAKAREILSQLTDALGGPAYLEVRERECD
                     GRRAQFGHAGELMGFIDFKDYWRYPDKHRVDYSKKGNIIDAFDGNQGWTMDRSGVSEE
                     PAQAVSDFAATVKYNVDNVLRSGLKDQSLNLNYAGQGIVDMRPVDWVEISDDQRTLRL
                     GIERSSHLLLHSVMISVDENTQERTQETTIYTNYQRKGGVMIALQISRERNGQRYYQA
                     FYDTCTFDPHLPEDMFTKAALEKRYAQVGNKKDKDRYRNARD"
     gene            complement(1783..2547)
                     /locus_tag="VII37_07480"
     CDS             complement(1783..2547)
                     /locus_tag="VII37_07480"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5069612.1"
                     /translation="MISLAGKAALITGGSRGIGAAAVKLFAQAGADVVFNYYKSKEPA
                     TQVEQDAAKHGTRVEAFKADVGRHADNKKLVDFAISRLGRLDIVVPNAGVWNSDDLPI
                     DKMTEKQWDDMIRVNLTSVYSIIHFSVPHMIKQKSGKIIPISSTAGQRGESLHTHYGA
                     SKGGIISLTKGLASELARHNILVNSVAPGWVATDMSNDVLNSKAGQKMVAAAIPLGRA
                     ATAEEIAGPILFLASDLANFMTGEVINVNGGSVLCG"
     gene            complement(2645..3421)
                     /locus_tag="VII37_07485"
     CDS             complement(2645..3421)
                     /locus_tag="VII37_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY5069613.1"
                     /translation="MSSMTPQKLQRPRVGIPWRTSAEEAEVNLAKTKNYQDAVRAAGG
                     EPVLLSLQRPQELQELLPSLDAFVLPGSPADVAPAEYGEVNRGKSEPPDAPRENTDRA
                     ILKHAFSEKKPVLAICYGCQLLNVYLGGTLIQDLREETDTPIAHTRHDIVPEPKDDPI
                     HAATFEEASRLAKIAGTVEAPVNSSHHQAVERVGKGLQVTAHAPDGTIEAVEWTDDSN
                     WVVGVQWHPERMFGDAFSERLFRDFIAAANQSRSVVAQKV"
     gene            complement(3501..4706)
                     /locus_tag="VII37_07490"
     CDS             complement(3501..4706)
                     /locus_tag="VII37_07490"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY5069614.1"
                     /translation="MTIGILGGGQLGYMLALAGYPLGLRFRFLDPSPEAPVGRIAQRV
                     TGDFDDEAALEKFASGLELVTYEFENVPVAATRFLSERVPVFPPPAALEVAQDRLKEK
                     ALFRKLGIATTEFAPVHNLKSLDAAVTSIGLPAVLKTSRFGYDGKGQWTLRTAADVEK
                     LKTEIPDALIKAQSMNGTQGSHDSAPFILERFISFTRELSILGVRARNGDIAFYPLVQ
                     NRHHGGILRLSIAPAPSLAPGLQREAEDIARRILTELDYAGVLAIEFFEYQGRLVANE
                     MAPRVHNSGHWTIEGAVTSQFENHLRAVLDLPLGSTNVHGSAAMLNLIGETPDSAEVL
                     AIPGAHLHLYGKSPRPGRKLGHITLWSSSFEKLSASLRALPPFFHQPSFCLDEPYASA
                     SHNEQSHFA"
     gene            complement(4703..>4961)
                     /locus_tag="VII37_07495"
     CDS             complement(4703..>4961)
                     /locus_tag="VII37_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEY5069615.1"
                     /translation="SLLSIAQMPGGIPVGTLAVGKSGAINSALLAAAILGIKYPAIRE
                     SLRKFREAQTNRVLETPDPSLLAQSKKNGPRPRTSKSRKSS"
BASE COUNT         1040 a         1365 c         1471 g         1085 t
ORIGIN      
        1 aatactgcga gtgtattcgt ttctcgtgaa ccatggcacg ccactcgctg cccattctgc
       61 gcggacgtca tcgggcgttc gaaacgaggt ggtgtaattt acgaccgtac cgccgcccaa
      121 gcattctccc gccaataatc cgacactgtg atccgcggtg gccgcagaac cgccctcgag
      181 ataaagccgc tggtaaccgg caagttccgc gccgtcgaag tcggcgtcgt cgtaatatcc
      241 tccggcttcg agaatgatta cgtcttttcc ggcggcagcg agcacggctg ccgcagtgcc
      301 gccaccagct cctgaaccga tgatgcagat ttcacaggat agcttggtgt cttgttgagg
      361 gacgagggtg gtcaacagac gcgacgagct agcgcgatta gcgagattcg gcggaccggg
      421 ataaccgatt ttgccccaca ccggtgtgcg cccaccctgc agctgcggca acatcacgta
      481 aaggtaagac accgctttgc gcaaagcctg gaaagcggcc cggcggcggc caagcgagct
      541 ttccgcccat gagagcaaca cgcgaacgcg ggcttcttct gacaatgtcg agaagcgcga
      601 tgcacgcttg atcgaaataa cgctgtgaaa ttgtgaatcc cagaagttca gcagctgcag
      661 gaattgttta tgatcgccgc ggcgcggatt gaaatccatg gcgtcggcgg tcgcttgcgg
      721 aacgcctaat tcagaagccg atggccagcc atccgccgcg ggcgcgaatg tgtcacaaat
      781 taattgcagg gcatgctgct ggcgtgaagt taatttcatc agcaggagca gactagcaaa
      841 agatggtcag cgcgggaagg aaacggcggg cacgcaacaa ctgaggcaga tcgagaacaa
      901 tttcaaataa tcagtctcgg gcattcctgt atctgtcttt gtcttttttg tttccgacct
      961 gcgcgtagcg cttctccagc gcggccttgg tgaacatgtc ttcgggaaga tgcggatcaa
     1021 aggtgcaggt gtcatagaac gcctggtaat aacgttggcc gttgcgctca cgggaaattt
     1081 gcaatgcgat catcactccg cctttgcgct ggtagttcgt atagatggtg gtttcctgcg
     1141 tacgctcctg cgtgttttcg tcaacggaga tcattacgct gtgcagcaat aaatgcgagg
     1201 accgctcaat tcccagccgc agggtacgct ggtcatcgga aatttccacc cagtcgacgg
     1261 gccgcatatc gacgatgcct tgtcccgcat aattcagatt caaactctgg tctttaagcc
     1321 cagagcgcag cacattatcc acgttgtatt tcaccgtcgc cgcgaaatca gagaccgctt
     1381 gggcaggctc ttcgctgacg ccggagcgat ccatggtcca tccctggttg ccatcgaacg
     1441 cgtcaatgat gttgcctttt ttggagtaat cgacgcgatg cttgtcgggg taacgccaat
     1501 aatccttgaa gtcgatgaag cccatgagtt cgccggcgtg gccgaattgc gcacggcggc
     1561 cgtcgcattc gcgctcgcga acttcgagat aggcgggacc gccgagagca tccgtcaatt
     1621 ggctgagaat ctcacgtgct ttcgcgacac gctgctcggg cagcatggta tccggattct
     1681 gttccggacg cattgcagca atggctccat tgctcgcgac atacgcagca gtcactagaa
     1741 caagcgcgca tgacaatcgc gtgcaaactt tcggaaactt gttcaaccgc acaagaccga
     1801 tcctccattt acattgatga cctcaccggt catgaagttg gctagatcgg aggccagaaa
     1861 cagaatcggc ccggcaattt cttcagcagt agcggcgcgc ccgagtggaa ttgcggcggc
     1921 caccattttt tggcctgctt tcgaattcag gacatcattg gacatatccg tggcaaccca
     1981 gccgggcgcg acggaattaa ccagaatgtt atgccgagcc agctccgagg ccagcccttt
     2041 ggtgaggctg atgatcccgc ctttggatgc cccgtaatgc gtgtgtagcg attcgccccg
     2101 ctgccctgcc gtggagctaa tgggaatgat tttgccggat ttctgcttga tcatgtgcgg
     2161 cacggaaaaa tgaatgatgg agtacacact ggtgagattc acgcgaatca tgtcgtccca
     2221 ctgcttctca gtcattttgt cgatgggcag atcgtcgctg ttccagacgc cggcgttggg
     2281 aaccacgata tccaggcgcc cgaggcggga tattgcgaag tccaccagct ttttgttatc
     2341 tgcgtggcgg ccaacatcgg ctttaaaggc ttccacgcgg gtaccatgct ttgctgcgtc
     2401 ctgctccacc tgggtggcgg gctctttgga tttgtagtaa ttgaaaacca cgtccgcgcc
     2461 ggcttgcgcg aagagtttta ccgccgccgc gccgatgcct ctggaaccac cggtaatcag
     2521 tgccgctttg cccgccaatg aaatcatctg tcccccaact gtcttttgaa gcctacgtat
     2581 aaacttttgc ccgaattttt cgggttacct gcggacgccg ctttattctg caggaacttg
     2641 gcagctaaac tttttgtgcg accacgctac gagattgatt ggccgccgca atgaagtccc
     2701 ggaataaccg ttcagaaaaa gcgtctccga acatgcgttc gggatgccat tgcaccccga
     2761 cgacccagtt cgaatcgtcg gtccactcca ccgcctcgat ggtgccgtcg ggagcatgcg
     2821 cggtgacttg caagcctttt ccaacgcgct cgacagcctg gtggtgcgag ctgttcacgg
     2881 gtgcctcaac ggtcccggca attttggcca ggcggctagc ctcctcgaaa gtggcggcat
     2941 gaatcgggtc gtccttcggc tcgggcacga tgtcgtgcct ggtgtgcgct atgggcgtgt
     3001 ccgtctcttc acgcaggtcc tgaatcaggg taccgcccag atagacgttc aggagctggc
     3061 aaccgtagca aatagctagg accggttttt tttcgctgaa agcgtgcttc aaaatggctc
     3121 gatcggtatt ttctcgcggg gcatcgggtg gctcggactt gccgcgattt acctcgccgt
     3181 attcggcggg cgcgacgtcg gcagggctcc cgggcaggac gaaggcatcg aggcttggta
     3241 atagctcctg aagctcttgt ggacgctgca gcgacaggag cacgggctcg ccaccggcgg
     3301 cccgcacggc gtcctgataa tttttggtct tggcgagatt gacttccgcc tcttccgcag
     3361 aggtccgcca aggaatcccc acgcgggggc gctgtaattt ttggggggtc atggaactca
     3421 tgaatcctag catcccagct agaacggcag caacccggcc aggtgctcac ttgtgggcca
     3481 aatcggccgg gtgctcagcc ctaggcaaag tgggactgtt cgttgtgaga ggcgctggcg
     3541 tatggctcat ccaggcaaaa cgacggctgg tgaaagaacg gcggcagggc gcgaaggctg
     3601 gcagaaagtt tctcgaaaga actgctccaa agggtgatgt gacccaactt gcggcccgga
     3661 cgaggcgatt taccgtagag gtgcagatgg gcgcccggaa tcgcaagaac ttcggcactg
     3721 tccggggttt caccaataag gttcagcatg gcggcggaac cgtgcacatt cgtggagccg
     3781 agaggcaaat ccaaaactgc ccgtaggtga ttttcgaact ggctggttac ggccccttcg
     3841 atcgtccaat gaccggaatt gtggacgcgg ggcgccattt cgttggccac caggcggccc
     3901 tgatattcga aaaactcgat agccaaaacg ccggcatagt cgagttcggt gagaatgcgc
     3961 cgagcgatat cttcagcttc acgctggaga ccaggggcaa gcgagggggc aggtgcgatg
     4021 gacagtcgca aaatgccgcc gtgatggcga ttctgcacca gagggtagaa ggcgatatct
     4081 ccgttgcgcg cgcgtacgcc gaggatggaa agctcacgcg tgaaagaaat gaagcgctcc
     4141 aggatgaacg gggccgagtc gtgtgagccc tgcgtcccgt tcatgctctg ggctttgatc
     4201 agagcgtcgg ggatttcagt ttttagcttc tctacatcag ccgcggttcg cagtgtccac
     4261 tgccccttgc cgtcgtaacc gaagcgagag gttttcagta ccgcgggcaa accgatggag
     4321 gttactgcgg cgtccagcga ttttaaatta tggactggcg caaactccgt ggtggcgatt
     4381 cccagtttgc gaaacaaagc tttttctttc agccggtctt gcgcgacttc caatgcagcc
     4441 ggcggcggaa agaccggaac gcgttcactg agaaatctgg tagccgcaac ggggacattt
     4501 tcgaattcgt aggttaccaa ttcgagcccg ctggcaaatt tttcgagcgc ggcctcatcg
     4561 tcaaaatcac cggtgacgcg ttgcgctatg cggcccacgg gcgcttccgg agaaggatct
     4621 aggaatcgga accgcaagcc aagtggatag cctgccagcg ccagcatgta tccgagttgg
     4681 ccgccgccga ggatgccgat ggtcacgagg atttcctcga cttacttgtg cggggccggg
     4741 ggccgttttt tttggattgc gcgagtaacg agggatcggg agtttcgagg acgcgattgg
     4801 tttgagcctc gcgaaatttg cgcagcgatt ctcgaattgc aggatatttg atgcccagaa
     4861 ttgctgcagc gagcagcgcg gagttgatgg ctcctgattt gcccacagcc aaagtgccca
     4921 ccggaatacc gccaggcatc tgggcgatgg aaagcaggga a
//
LOCUS       DATCBB010000676         1786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_216784, whole genome shotgun sequence.
ACCESSION   DATCBB010000676 DATCBB010000000
VERSION     DATCBB010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216784"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VII37_07500"
     CDS             <1..207
                     /locus_tag="VII37_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5069616.1"
                     /translation="FAEDIGFAQRDVIAEVNRTAISSIEDYRSAIGKLKAGDSVVFKV
                     LRHQDTDRVLTVFLPGVVPAEDKR"
     gene            242..736
                     /locus_tag="VII37_07505"
     CDS             242..736
                     /locus_tag="VII37_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY5069617.1"
                     /translation="MNRHLRPMHLILLSLLMVGLGVSPCLLSAQTTAKKKAKSSSSSH
                     KGKSTKTSRRQVGQKAPTPQRITEIQQALAKNGAYAGTPNGKWDGSTAEAMKKFQGAH
                     GLNASGKLDAKTLQQLGLGSQTAGIAAPTPAVRISSTNNPQGSLVDPQNDTLSSKYKA
                     GSKQ"
     gene            869..>1786
                     /locus_tag="VII37_07510"
     CDS             869..>1786
                     /locus_tag="VII37_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5069618.1"
                     /translation="MATAASPRVFKNFINGEWVESRSGKAYENRNPANTEELVGMFVS
                     SSSEDVSAAVDAAKEAYKSWRLVPAPKRAEILYRTAELLLQRKEELSRDMTREMGKVL
                     AETRGDVQEAIDMTYYMAGEGRRLFGQTTPSELPNKFAMSARQSIGVCGMITPWNFPM
                     AIPSWKMMPALVSGNTVVLKPAEDTPLSSYHLVQILMEAGLPNGVVNLVSGDGPGAGA
                     PLAGHKDVPVVSFTGSTAVGRIIAQTCAPDFKHCSLEMGGKNIIIVMDDANLDLAVDG
                     AIWGGFGTTGQRCTAASRVAVHKSVYREFI"
BASE COUNT          422 a          418 c          570 g          376 t
ORIGIN      
        1 tttgctgaag acatcggatt cgcgcaacgg gacgttattg ccgaagtgaa ccgaacggcg
       61 ataagctcca tcgaagacta ccgaagcgcc attggtaaac tgaaggccgg cgacagcgtg
      121 gtgttcaagg tgttgcgtca tcaggatacc gaccgggtac tgacagtgtt tctaccgggc
      181 gtagtgccgg cggaagataa acgataaagg cctcgattta attcgagctt agaaaaacta
      241 tatgaaccga cacctacggc cgatgcacct gatcctgctg tcactactca tggtgggatt
      301 gggtgtgtcg ccgtgtttgt tgtcggcgca gactaccgcc aagaaaaaag ccaagagcag
      361 cagtagctcg cataagggca agtccacaaa aacttcacgt cgtcaggttg ggcagaaagc
      421 ccccacgccg caacggatta ctgaaattca acaggcgcta gcgaagaatg gcgcgtacgc
      481 gggaacgccg aatgggaagt gggatggttc caccgctgag gctatgaaga agtttcaagg
      541 cgcacatggt ttgaatgctt ctggaaagct ggacgcaaag accttgcagc aacttgggtt
      601 gggctcgcag acggcaggca tcgcggctcc cacgcctgcg gttagaatct cttctacaaa
      661 taatccacag ggttcgttgg ttgaccctca gaacgatact ctgagcagca agtacaaggc
      721 cggcagcaaa cagtaattcg tcactgcgat ttagtgtttt gtgaaaaggg ttcctgcccg
      781 tggtgtgcct gcgagcgtca aatcgattat catattaaat atcgtcccgc gcattctctg
      841 cgcttgaagt gtgcattcag gaggaagtat ggctacagcg gcatcgcctc gcgtttttaa
      901 aaattttatc aatggcgaat gggtggaatc gcgcagtggc aaagcctacg aaaatcgcaa
      961 ccctgcgaat accgaagaac tggtgggcat gtttgtgtct tcgagctcgg aagacgtgag
     1021 tgccgcggtg gatgcggcca aggaggctta taaaagctgg cggttggtgc ctgctcccaa
     1081 gcgcgcggag attttgtatc gcactgcgga attgctgctg caacgcaagg aagagctctc
     1141 gagagatatg acgcgggaga tgggtaaagt actggcggag acgcgcggag acgtgcagga
     1201 agccatcgac atgacctact acatggcggg agagggacgg cgtttgtttg ggcagaccac
     1261 tccttcggag ctgccgaaca agtttgcgat gagcgcgcgg cagtccattg gagtgtgcgg
     1321 catgatcacg ccgtggaatt ttccgatggc gattccgtcg tggaagatga tgccggcgct
     1381 ggtgagcgga aacaccgtgg tgttgaagcc agcggaggat acgccattgt cgtcgtatca
     1441 cctggtgcaa attttgatgg aggcgggatt gccgaacggc gtcgtgaacc tggtaagcgg
     1501 ggatgggcca ggtgcgggcg cgccgctcgc ggggcacaag gatgttccgg tggtcagctt
     1561 caccgggtcc actgcggtgg gaaggatcat tgcgcaaact tgtgcgccgg attttaagca
     1621 ctgtagcctg gagatgggcg ggaagaacat catcatcgtg atggatgatg cgaatcttga
     1681 tctggcggtg gacggagcaa tctggggcgg attcggcaca actgggcagc gttgcacggc
     1741 ggctagccgg gtggcggtgc acaaaagcgt ttaccgcgaa tttatt
//
LOCUS       DATCBB010000677         1193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_442902, whole genome shotgun sequence.
ACCESSION   DATCBB010000677 DATCBB010000000
VERSION     DATCBB010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442902"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VII37_07515"
     CDS             <1..529
                     /locus_tag="VII37_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5069619.1"
                     /translation="AQYYHHNLPRTLITSGGLGTMGFALPAAIGVKFAKPDAEVWVVV
                     GDGGFQMTMSELATIVQEKINIKIAIINNGFLGMVRQWQEFFYDKRYVATPLVAPDFA
                     ALANAFGIRGERIATRADVLPCIRAARQSPETVLIDFRVEQEDSVYPMVAAGASLHDM
                     IRRPDSPLVETATEP"
     gene            526..1101
                     /gene="ilvN"
                     /locus_tag="VII37_07520"
     CDS             526..1101
                     /gene="ilvN"
                     /locus_tag="VII37_07520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY5069620.1"
                     /translation="MKKIEETMQTLVAYVENKPGVLNRVSSLARRLAINIDSLTVGPT
                     ENEEIARMTIVAHTDEKGAHRLEASLEKLVDVLLAENISNRSVLERDLALIKVLADQK
                     NRPHLMELIKVFRARVVDVAPESLILEVSGTVDKIDGLLEVLRPFGVLEMARTGRIAM
                     TRGGDLLRPPVVNEPEVTANPGTRAGITVTN"
BASE COUNT          280 a          332 c          323 g          258 t
ORIGIN      
        1 ggcgcagtat taccaccaca atttgccgcg tacgctgatc acttccggtg gactcggcac
       61 catgggtttc gctctccccg ccgcgatagg cgtcaaattc gccaagcctg acgcggaagt
      121 ttgggtggtc gtcggcgacg gcggattcca aatgaccatg agcgagctag ccaccatcgt
      181 gcaggaaaaa atcaacatca agatcgccat catcaacaac ggcttcctgg gcatggtccg
      241 ccagtggcag gaattttttt acgataaacg ctacgtcgcc actccactcg tagctcccga
      301 ttttgctgcg ctggccaacg cttttggcat acgtggagag cgcatcgcta ctcgagccga
      361 cgtgttgccc tgcattcgcg ccgcacgcca atccccggaa acagtactca tcgattttcg
      421 cgtggagcag gaagactccg tttaccccat ggtggcagcg ggcgcttcgc ttcacgacat
      481 gattcggcga ccggatagtc cgcttgtgga gactgcgact gagccatgaa aaaaatagag
      541 gaaaccatgc aaacactcgt cgcttacgtg gagaacaaac caggcgtcct gaaccgcgtc
      601 tcgtctctgg cccggcgttt ggccatcaat attgattcgc ttactgtagg ccccacggaa
      661 aatgaagaga tcgcgcgcat gaccatcgtg gcacacaccg acgaaaaggg cgcgcaccgc
      721 ttggaggcca gcctcgaaaa attagtggat gtattgctgg ctgaaaatat ttccaaccgc
      781 tccgtccttg aacgcgatct ggcgctgatt aaagttttgg ctgaccagaa aaatcgtccg
      841 caccttatgg aattgatcaa agtattcaga gcccgggttg tggacgttgc gccagagtcc
      901 ctgattctgg aagtctccgg caccgtggat aagattgacg gcctgcttga agtcttgcgc
      961 ccctttgggg ttttggaaat ggcgcgcact ggaaggattg ccatgactcg tggaggcgat
     1021 ttacttcgtc caccggtcgt taacgagcca gaagttaccg caaatccagg gactcgcgcc
     1081 ggaataacgg tcacgaactg acaattgctt tagcgggctt ttcgtaaatc tgtagaacgc
     1141 acgaaattta tcgcggctgc gcaggccgcc caaggaaaac gcatggcaaa act
//
LOCUS       DATCBB010000678         1699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_248197, whole genome shotgun sequence.
ACCESSION   DATCBB010000678 DATCBB010000000
VERSION     DATCBB010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248197"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VII37_07525"
     CDS             complement(<1..557)
                     /locus_tag="VII37_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5069621.1"
                     /translation="MGILLGLLTAVTWGGSDFLARFATHRIGTLRAMFYMQATGLVLL
                     SICLPWLGGWGHLHDGSGWQPWAWGIFAGCINAASTLTLYRSFEVGKLSVVAPLSSSY
                     PALTVILSLLTGEHLRAARGAGIIFTMLGVVLVARGEQEPHKDDVEGNARKGKGVVWA
                     LCSACGFGVLFWLLGIRIVPRVGAP"
     gene            complement(632..1444)
                     /locus_tag="VII37_07530"
     CDS             complement(632..1444)
                     /locus_tag="VII37_07530"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5069622.1"
                     /translation="MTGRLPENLQEKLSLRLRYYEDIGIREFFRDRIGPVHLEPLEQV
                     VPQTQLTLTSEGSETMNETPAPRPLITSIPKIGEGTLQTIREDIGDCIRCKLHKGRTK
                     IVFGDGHPQAKLLFIGEGPGRDEDLQGLPFVGRAGKLLTQMIEAMGLRRSDVYICNVV
                     KCRPPENRAPERDEVAACSPFLFRQIDLVAPKVIVCLGSIAAQTILETARGISQFRGQ
                     WLEFRGRKLMATYHPAYLLRNPAAKSEVWKDLQKVMAELGLEVKKKPAQTEA"
     gene            complement(1453..>1699)
                     /locus_tag="VII37_07535"
     CDS             complement(1453..>1699)
                     /locus_tag="VII37_07535"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY5069623.1"
                     /translation="GQNARKKLTAKNVDLIVANDVTAEGAGFDHNTNVVSLYFRDGRD
                     VTLPKMSKADVAQRVLDEIVRLRGALRTKPAKRLSVV"
BASE COUNT          390 a          522 c          407 g          380 t
ORIGIN      
        1 tgcggcgcgc ccacccgcgg cacaatgcga atccccaaca accagaacag caccccaaat
       61 ccgcacgcgg aacaaagcgc ccacaccact cccttgccct tgcgcgcatt tccctctaca
      121 tcatctttgt gcggctcttg ctcaccgcgc gccaccagca ccactccaag catggtgaaa
      181 attattcccg ctcctcgcgc ggcacgcaga tgctcgcccg tgagcagcga caaaattacc
      241 gttaacgctg ggtagctgga cgaaagcggc gcgaccactg aaagtttccc gacttcaaac
      301 gatcggtaaa gtgtcagcgt agaagcggca ttaatgcatc cggcaaatat tccccaggcc
      361 catggctgcc acccggatcc atcatgcaaa tgcccccaac cgcccagcca aggcagacag
      421 atactcaaca acaccagccc ggtcgcctgc atgtaaaaca tggcccggag tgtgccaata
      481 cggtgggtgg caaatcgcgc aagaaaatcc gagccgcccc aagtcacggc ggtgagtagg
      541 cccagcaaaa ttcccataaa gttagtttct catcaccagg caggtagtaa gacgctgaac
      601 taaattcttc cgagcagttc tatttcaacg atcaagcttc tgtctgagca ggctttttct
      661 tcacttcaag tcccaactcg gccattactt tttgcaaatc tttccaaact tcgcttttcg
      721 ccgcgggatt acgcagcaaa taagcaggat gataggtggc cattaacttc ctgccgcgaa
      781 actcaagcca ctgaccgcga aactgcgata ttcctcgtgc ggtctcgagt atggtctgcg
      841 ccgcgatgct gccaagacaa acgattactt tgggagcaac caagtcaatt tgcctgaaca
      901 gaaatggcga gcacgccgcc acttcatctc tttcgggtgc gcgattctcc ggaggccggc
      961 acttcaccac attgcatatg tacacgtcgc tgcgccgcag tcccatagct tcgatcatct
     1021 gcgtaagcag tttcccagcg cgtcccacga acggcagccc ttgtaaatct tcatcgcgtc
     1081 cgggaccttc cccgataaaa agcaatttcg cctgcggatg cccatcgcca aaaacaattt
     1141 tcgtgcgtcc cttatgcagc ttgcaccgta tacagtcgcc gatgtcttcg cgaattgtct
     1201 gaagcgtgcc ttcgccaatt ttgggtatgg aggttatcag cggtcgtggt gcgggtgttt
     1261 cattcatcgt ttcagagcct tcagaggtca gggtgagttg ggtctggggc acaacttgct
     1321 cgagcggctc taaatgaacc ggcccgatcc ggtcgcggaa aaattcccga atgccgatat
     1381 cttcgtaata tcgcagtcgc aaagaaagtt tttcttgcag gttttcagga aggcgaccgg
     1441 tcatgatggt tatcagacca ccgaaaggcg cttggcaggc ttcgttcgca atgctccgcg
     1501 caatcgcaca atttcgtcca gcacgcgctg cgctacgtcg gccttactca ttttcggcaa
     1561 cgtcacgtcg cgcccgtctc ggaagtacaa cgagactacg ttggtattat ggtcgaaccc
     1621 cgcgccttcc gcggtaacgt cgttcgccac gatcagatcc acattctttg cagtcaattt
     1681 cttgcgcgcg ttttgtccc
//
LOCUS       DATCBB010000679         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_477895, whole genome shotgun sequence.
ACCESSION   DATCBB010000679 DATCBB010000000
VERSION     DATCBB010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477895"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VII37_07540"
     CDS             complement(<1..300)
                     /locus_tag="VII37_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5069624.1"
                     /translation="MRRLVPLILAATLAFLTSCRAPKPQIVVGSKFFTEQVVLAELLA
                     QHIEARTGIHVERKTNLGGTLLVHKAMQAGQVDLYVEYTGTALNAVLNERPTGDSS"
     gene            complement(375..1007)
                     /locus_tag="VII37_07545"
     CDS             complement(375..1007)
                     /locus_tag="VII37_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069625.1"
                     /translation="MSPWTFMVTHRSEIWSATLDHVGLVLIAMSIAIAIAVPLGMFIV
                     QRPALRTLSLGVASVFQTIPSLALFGFLIPIPFIGGIGPRTAIVALVLYALLPILRNT
                     YVGLTSIDPAVLEAAEAMGMTDTQILIRVRLPLALGIILAGIRTATVITIGVATIAAA
                     IGAGGLGTFIFRGVAMVSDAVILAGAIPAALLAILADLSLALIERRLRAQ"
     gene            complement(1004..1726)
                     /locus_tag="VII37_07550"
     CDS             complement(1004..1726)
                     /locus_tag="VII37_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5069626.1"
                     /translation="MTYSVKGRSAPLISKLSVSVNSRETLVLLGRSGSGKTTLLRLIN
                     RMLVPSSGEVLVEGKSTVDWDAIRLRRHIGYVIQEAGLFPHFTVAENVGLVPGLEKWD
                     PTRISKRTDELLRIVGLDPKEFAQRRPAQLSGGQRQRVGVARALGADPSILLMDEPFG
                     ALDPVTRSELQHEFSELARRLGKTIVFVTHDLREALLLGSRIVLLETGRIVANAKPEE
                     FLRIDHPEVKAFSATLHTGAPA"
     gene            1924..>2560
                     /locus_tag="VII37_07555"
     CDS             1924..>2560
                     /locus_tag="VII37_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5069627.1"
                     /translation="MAAITSAVRLRHNLSARLTDARKRSDELFAIVRSESMYDRPIPE
                     RHRIIFYVGHLEAFDWNLLAERAFGLRPFQRTFDQLFSFGIDPVGGGLPTDTPADWPS
                     REEITKYVRGVRESLDHAIDEALSKPDEGHPQLKTMLEVAIEHRLMHSETLAYMLHRL
                     PTEKKHGEAIVRTRANARTRTELIEIPAGRATLGLEMKPGNEFGWDNEMEGH"
BASE COUNT          617 a          708 c          744 g          491 t
ORIGIN      
        1 ggaggaatcc ccggtcgggc gttcgttcag gacagcgttg agtgccgtgc cggtgtactc
       61 gacataaagg tcaacttgtc cggcttgcat ggctttgtgc accagcagag tgccgccaag
      121 atttgttttg cgctccacgt gaataccggt gcgcgcttcg atgtgctgcg cgagtagctc
      181 cgcaagaaca acttgctccg tgaaaaattt agagccgact acgatttgag gttttggcgc
      241 gcgacaagaa gtgaggaacg ctaatgttgc ggcaaggatc aagggaacga gtctgcgcat
      301 cgcgcactaa tttctcacgt gtaagagtaa acaggaagta cgaactaaat cggttggcac
      361 gtgtgcggta agcattattg ggcgcgtaga cggcgctcga ttaatgcgag cgacaggtcc
      421 gcgagaattg ctaataacgc tgctgggatg gccccagcaa gaatcactgc atcgctcacc
      481 atggcgactc ctcgaaagat gaacgtgcct aaaccacccg cgccgatggc cgcggcgatg
      541 gtggctactc caatcgtgat tactgtcgcg gtgcggatgc ccgccaaaat aattcccaga
      601 gcgagcggca agcgcacacg aatgagaatc tgcgtgtcgg tcatacccat agcttcagca
      661 gcttccagca cggcgggatc gatgctcgtg agtcccacgt aggtgttgcg aagaatgggc
      721 agcagcgcgt agagcaccag cgcgacgatg gcagttcgtg gaccgatgcc gccaataaat
      781 ggaatgggaa ttaagaatcc gaacagcgcc aagctgggaa tggtctgaaa tacgctggca
      841 acacccagag aaagagtccg caacgccggc cgctgcacga tgaacatccc tagcggcacc
      901 gcaatcgcta tggcaatcga cattgcaatt aaaacgagcc ccacgtgatc aagcgtggcg
      961 ctccaaattt ccgagcggtg cgtgaccatg aatgtccagg ggctcaagcc ggcgctcccg
     1021 tgtgaagcgt agcgctgaac gctttcactt ccggatgatc gattcgcaga aattcctcag
     1081 gcttcgcgtt ggcgacgatt cgcccggttt ccagcagcac gatacgcgac cccaacagca
     1141 gagcttcacg caggtcgtga gtgacgaaaa cgatagtctt gcccaggcgg cgggctagct
     1201 cagaaaattc gtgctgcaac tccgaccgcg tcaccggatc gagtgcgcca aaaggttcgt
     1261 ccatcaacaa gatggatggg tcggcaccga gggcgcgagc aacaccgacg cgttgacgct
     1321 gcccgccgga gagttgcgca ggacgccgct gtgcaaactc cttcggatcc agaccgacga
     1381 ttcgtagcaa ttcgtccgtg cgcttggaaa tacgcgtggg atcccatttt tccaatccag
     1441 gaaccaaacc aacgttttct gccaccgtga aatgtggaaa caagccggcc tcttgaatca
     1501 cataaccaat atgccggcgc agccggatgg catcccaatc gacggtggat tttccttcca
     1561 ccaacacctc tccggacgag ggaaccagca ttcgattgat gagcctgaga agggtcgttt
     1621 ttccactccc gctccggcca agtaagacca atgtttccct agagttaacg gacaccgaaa
     1681 gcttcgagat caggggtgcg gacctaccct taacgctgta tgtcacggcc tggaacctaa
     1741 tgatagcgtc agcctttggg ggaggagaaa ccgcgtccat catgctaacc cggccatcgt
     1801 aagcttgtcc gcatcgggcg cctatcatag tgcatctaac tcctcgcaat aaatcgaata
     1861 cacgttcgaa ctacggcatg gttaaatgcc gacccagata atgcagaccg agggctacaa
     1921 atcatggcgg cgataacgag cgcagttcgc ctgcggcaca atctttcggc gcggttgacc
     1981 gacgcgcgaa agcggagcga cgaacttttt gcgattgtgc gcagcgaatc catgtacgac
     2041 cgtcctatcc ctgaacggca ccgcattatc ttttatgtcg ggcacctgga agcgttcgat
     2101 tggaacctgc tggcggagag agcgtttggg ctgaggccct ttcagcgcac cttcgatcag
     2161 ttgttttcct ttgggatcga tccggtgggc ggaggactgc ccacggatac gcctgccgac
     2221 tggccaagcc gcgaagaaat cacaaaatat gtgcggggtg tgcgcgagag ccttgatcac
     2281 gcgattgatg aggcgctcag caagccggac gagggtcatc cgcagctgaa aaccatgctg
     2341 gaggtggcca tcgagcaccg gctgatgcat tcggagacgc tggcgtacat gctgcaccgc
     2401 ctgccaaccg agaaaaaaca tggcgaagcg atcgtcagaa ctcgcgccaa tgcgcgcacc
     2461 aggacggaac tgattgagat tccagcgggg cgcgctacgc tcgggctgga gatgaaaccc
     2521 ggaaatgaat tcggatggga caacgagatg gaaggacacg
//
LOCUS       DATCBB010000680         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_152722, whole genome shotgun sequence.
ACCESSION   DATCBB010000680 DATCBB010000000
VERSION     DATCBB010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152722"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VII37_07560"
     CDS             complement(<1..647)
                     /locus_tag="VII37_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5069628.1"
                     /translation="MATATTTPQGTKGGSFLLESPRPQDVFTPADLNDDQRLIGQSAE
                     EFVIKEVMPHVKNLEAKKPGLLPELVKKAGELGLLSGGIPEQYGGAGLDKVATTVLTE
                     KLSIYGGFAVTHGAHAGIGTLPIVYFGTEEQKKKYLPKLATGEWIGAYCLSEPQAGSD
                     AQNSLTRAELTPDGKHYILNGQKMWITNGGFADIYIVFAKIGGEKLSAFIVERTFP"
     gene            complement(806..1195)
                     /locus_tag="VII37_07565"
     CDS             complement(806..1195)
                     /locus_tag="VII37_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEY5069629.1"
                     /translation="MTIPNLRQDQRMDQIMAVLLRSGVLLAASLVFIGGIVYLSRHDL
                     PAINYRVFQGEPEELRNVGGILREAGKFHGRGLIQLGLLILIATPVARVLFSVFAFLY
                     ERDWIYVAITMIVLALLCYSLFGGGAL"
     gene            complement(1197..2033)
                     /locus_tag="VII37_07570"
     CDS             complement(1197..2033)
                     /locus_tag="VII37_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5069630.1"
                     /translation="MHTLEFTLLVGMGSLLAGFLGSLTGLGGGVVIVPMLTLLFHVDI
                     RYAIGAALVSVIATSSGAAAAYVKEGYSNVRVGMFLEIATTVGALVGAAVALHLHVSV
                     IAIIFGLALIYSAFSSLREKSEHRTDVAPDRIATRLRMDSSYPTDKGMKQYHVRGVPL
                     GFALMFGAGVLSGLLGIGSGAVKVLAMDQAMKLPFKVSTTTSNFMIGVTAAASAGIYL
                     SRGYIDPGIAMPVMLGVLGGSLVGARILAGAKVRTLRLVFAVVIGFLAVEMIYNGFAG
                     KM"
BASE COUNT          538 a          679 c          579 g          477 t
ORIGIN      
        1 gggaatgtgc gttccacaat gaacgcactc aatttttcgc cgccaatctt ggcgaacaca
       61 atataaatgt ccgcgaaccc tccattggta atccacattt tctgcccgtt caaaatgtag
      121 tgtttgccgt cgggcgtaag ttcggcgcgc gtcaaggaat tctgcgcatc ggagccggcc
      181 tgcggttcgg aaaggcaata ggcaccgatc cattcgccgg tagccaactt gggaagatat
      241 tttttctttt gctcttcagt gccgaaatac acgataggca gcgtgccgat gccggcgtgg
      301 gctccatgcg ttacggcaaa gccgccgtag atggaaagtt tctccgtcag aactgtcgtg
      361 gccaccttat cgagtccggc accaccgtat tgttctggga tgccgccgct caagagccca
      421 agctcaccgg cctttttcac cagttccggc agcaagcccg gttttttggc ctccagattc
      481 ttgacatgcg gcattacttc cttgatgaca aattcttccg ccgattgtcc gatcagtctt
      541 tgatcgtcgt ttaagtccgc aggggtgaaa acgtcctgag gccgcggcga ttccagtaga
      601 aagctgccgc ctttagttcc ttgtggcgtg gtggttgcgg tggccatggt caggggctcc
      661 tagttgggca cggctgtgct cgcttcaatt atctcatctt tgcggaaggt ccggcgtgcc
      721 gagtgacatc gacagagtac aggtaaacgc ctgcacttat tggcaggttt tcttatggcg
      781 cccggtctaa ttcacggcgg gcagcttaca acgctccgcc accgaacaag ctgtagcaca
      841 gcaaggccaa cacgatcatc gtgatcgcga catatatcca atccctttca tacaaaaacg
      901 caaaaaccga aaaaagcacc cgagccacag gtgtggcaat caatatcaga agccctaatt
      961 gaatcaatcc ccgcccgtga aattttccag cctcacggag aattccgcca acattgcgca
     1021 actcttccgg ttcgccctga aagacgcggt aatttatcgc cggtaaatca tgacgtgaca
     1081 ggtaaacaat tccgccaata aataccagac tggccgcgag cagaactcca ctacgcaaga
     1141 gcaccgccat gatctgatcc atgcgttgat cctggcgtag attcggaatg gtcatttcac
     1201 atcttccccg cgaagccgtt ataaatcatt tccactgcga gaaatccgat gacaacggcg
     1261 aagacgagtc gcagggttcg caccttggcg cctgccagga tgcgtgctcc gacaagcgat
     1321 ccgcccaaca ctccgagcat caccggcatc gcgatgcccg ggtcaatata tccgcggctc
     1381 aaataaattc cagcgctggc cgcggccgtc acgccaatca taaaattgct tgtagtggtc
     1441 gacactttaa acggcagctt catggcctga tccatggcca aaacttttac tgctcccgat
     1501 ccaattccca ataagccgga cagaacgccc gctccgaaca tcagcgcaaa gcccagcggc
     1561 actccgcgga cgtgatattg cttcatcccc ttatccgtcg gatagctgct atccatgcgc
     1621 agcctggtgg caatgcggtc gggcgccaca tcggtccggt gctcagattt ttcgcgcagg
     1681 ctggagaacg ccgagtaaat cagcgcaagc ccaaagataa tggcaatcac ggacacgtga
     1741 agatggagcg ccaccgccgc acctaccaac gctcccacgg ttgtcgcgat ttccaggaac
     1801 attcccacgc gcacgttcga atacccttct ttcacgtacg cagccgcggc tcccgaagaa
     1861 gtcgcgatga cggaaacgag cgcggcgcca atcgcgtagc gaatgtccac gtggaacaac
     1921 aaagtgagca ttggaacgat gaccacgccg ccgcccagtc cggtcagtga gccgagaaac
     1981 ccggccagca gcgaacccat gcccaccagc agggtgaatt ccagcgtgtg caagagtttc
     2041 tccgggagcg ctgatccgtt taagctcagc gcgcacaatt cacaggtggc agacgaactt
     2101 cttacaacgg ttgagatgca aaaatactga agaccggctc aaaaattcag gagagcagct
     2161 cgaaaatccc cgccgcgccc attccgcctc ccacgcacat ggtcaccatg ccgtagcggc
     2221 ccttgcgacg ttgcagatcg cgaatgatgg tggccgtaag ttttgcgccg gtg
//
LOCUS       DATCBB010000681         1069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_378398, whole genome shotgun sequence.
ACCESSION   DATCBB010000681 DATCBB010000000
VERSION     DATCBB010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378398"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VII37_07575"
     CDS             <1..687
                     /locus_tag="VII37_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5069631.1"
                     /translation="ETLQFFYDLGIPVANGYGLTEAGTAITVNDLKPFRADTVGKPLP
                     GMEVRVMHPDAEGIGEVAVRSKTVMSGYLNEPELTAETIVNGWLMTGDLGRFDLTGHL
                     QLFGRKKNMIVTEEGKNIYPEDVESVFESLAVKEFCVMAVNYIWPQRSMVGEQLVLVI
                     HLEPGQSLTKDLRQEIAARNNKLLNYKRVSGIVLTTEDFPRTASMKIKRNVLAEGLAK
                     LDRSSAVLEL"
     gene            737..>1069
                     /locus_tag="VII37_07580"
     CDS             737..>1069
                     /locus_tag="VII37_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEY5069632.1"
                     /translation="MERFFAIVNPAAGGGRSAKLAGPALERLRKSGLEVDEATTGAPG
                     DAVILAREAYTEGYRRFLAVGGDGTGHEILNGIFGRAASAQRAGVQHASTERRAAAQH
                     ATAESAAAD"
BASE COUNT          259 a          252 c          349 g          209 t
ORIGIN      
        1 gagacgctgc aattttttta cgacttggga attcccgtcg ctaacgggta tggccttacg
       61 gaagcgggaa ccgccattac cgtcaatgac ttgaaaccgt ttcgcgcgga caccgtgggc
      121 aagccgcttc cggggatgga agtgcgcgtg atgcacccgg acgcggaagg catcggcgaa
      181 gttgcggtgc gcagtaaaac ggtgatgtca ggttatctga acgaaccgga attaacggcg
      241 gaaaccattg tgaatggctg gttgatgacc ggggatctgg ggcgcttcga tctcaccggg
      301 cacctgcaac tcttcgggcg taagaaaaac atgatcgtca cggaagaagg gaaaaatatt
      361 tatcccgagg acgtggagag cgtgttcgaa agtctggcgg taaaggaatt ttgcgtgatg
      421 gcggtgaatt acatttggcc gcaacgcagc atggtgggcg agcagttggt gctggtaata
      481 catctggaac cgggacaatc cctgacgaaa gatttgcgcc aggaaattgc agcgcgaaat
      541 aacaagctct taaactacaa gcgcgttagc ggaatcgtgc tgaccaccga agattttccg
      601 cgcaccgctt ccatgaaaat taaaaggaat gttctggcag aggggctcgc gaaactggac
      661 cgcagcagcg cagttctgga actgtaaggg ccacacagca cagcacaaaa tcatttttgg
      721 gagagaatag gccggcatgg aacgattttt tgccatcgtg aatccggcag ccggtggagg
      781 tcgctccgcg aaactggcgg gccctgcgct ggagcgtttg cgcaaaagcg ggctggaagt
      841 ggatgaggcg accaccggcg ctcctggaga tgcggtgatc ctcgcgcgcg aagcgtatac
      901 ggaaggttac aggcgctttc ttgcggttgg cggcgatggc accgggcatg aaattttgaa
      961 tgggattttt ggcagagcgg ctagtgcaca acgtgcgggt gtgcaacatg cgagcacgga
     1021 acgccgggcc gcggcacaac atgcgacggc tgaaagtgcc gcggccgat
//
LOCUS       DATCBB010000682         1416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_346433, whole genome shotgun sequence.
ACCESSION   DATCBB010000682 DATCBB010000000
VERSION     DATCBB010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346433"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            650..>1416
                     /gene="cysD"
                     /locus_tag="VII37_07585"
     CDS             650..>1416
                     /gene="cysD"
                     /locus_tag="VII37_07585"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY5069633.1"
                     /translation="MAETSKESVNTSPTNGGERAALLTPQKINYLRALEAESIHILRE
                     AVAEFARPVMLYSIGKDSSVMLRLAQKAFFPGKIPFPLLHIDTSYKFPEMVEFRDRYA
                     REIGAELIVHKNQEALDAGANPFVLGTQKCCGLLKTKSLLDALAEGDFTAAFGGARRD
                     EEKSRAKERFFSFRDSFGQWDPKNQRPEIWNLYNARLNPGENMRVFPLSNWTEMDVWE
                     YIKQEKLQVPTIYFSHQRQCVRRLGQWLPVNDLVPPKP"
BASE COUNT          313 a          377 c          432 g          294 t
ORIGIN      
        1 gtcgtaaacc acttcgagcg gcgagaggca ctcgtcgcaa aatgagcgcg cctgattgcc
       61 ccagagcttt ccgcattctc ggcatttcaa ctgataatgt tcggccattt ggcctcctgc
      121 gctcggcccg ccaaggaatg agatggcgca gcgggcgaat cggctccaga tttatggctg
      181 gtagactgcc gcaggtttgg cggcgcgaga gggggtaaat tgaaaaaccc tccgcgccaa
      241 agagggggag ggctcaaaaa actcgatctg agtgctcgtc cgactcttta gcagtttttt
      301 acgtggagca agttgcctta aatcgccacg tgtccagcga acgaagtata ggaattaact
      361 tcgaccatgt caaggcgttt ttgccggaaa gctttcgcct tgctagagat agaagattcg
      421 ggtactctga gggcgtgagg cttgcgcagc attccgcgtc gaatacggtt ctgggctgtt
      481 gttgtacggt catgggctag gttcttcgag agtgggcaag atactcaatc taaagctgct
      541 taagtagatt tgcaagccgc tacccaccgc cgcaggacgt ggccggtggg tttaatgttt
      601 taggggcagc aagaaagccc ttcgagttgg gtggcaggaa tcgacatcaa tggctgagac
      661 atcaaaggaa tctgtgaaca cgtccccaac caatggcggc gagcgggctg cgcttttgac
      721 gccgcagaag atcaactacc tcagggcgct ggaggcggag agcattcata ttctgcgcga
      781 ggcggtagcg gagtttgcgc ggccggtgat gctctactcg atcgggaaag attcctcggt
      841 gatgctgcgg ctcgcgcaga aagcgttctt tccggggaaa attccgtttc cgctgctgca
      901 catcgatacc agctacaaat ttcccgagat ggtcgagttt cgcgatcgct acgcgcggga
      961 aattggcgcg gagctgatcg tgcacaagaa tcaggaagcg cttgatgcgg gcgcgaaccc
     1021 gtttgttctg ggcacgcaga agtgttgcgg tctgctgaaa acgaaatcgc tccttgatgc
     1081 gctggctgag ggcgatttca cggcggcgtt tggaggcgcg cggcgcgacg aggaaaaatc
     1141 ccgcgccaag gaacgcttct tcagcttccg cgacagcttc ggccagtggg accccaagaa
     1201 ccagcgcccc gagatctgga atctctacaa cgcccggctg aatcccggcg aaaacatgcg
     1261 cgtcttcccg ctcagcaact ggacggagat ggacgtgtgg gaatacatca agcaggagaa
     1321 gctccaggtc ccgaccatct acttcagcca ccagcgccaa tgcgtccgca ggctgggcca
     1381 atggctgccc gtcaatgatc tcgtgccgcc gaaacc
//
LOCUS       DATCBB010000683         1091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_411882, whole genome shotgun sequence.
ACCESSION   DATCBB010000683 DATCBB010000000
VERSION     DATCBB010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411882"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VII37_07590"
     CDS             complement(<1..524)
                     /locus_tag="VII37_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5069634.1"
                     /translation="MLSLLEELNPEQRVAAEITEGPLLILAGAGTGKTRAITFRMANL
                     IANGVPSEAILAVTFTNKAAQEMRTRVSDLLLRAGVPPSSPWLSTFHSLCARLLRREA
                     VSAGLPRDFVIYDDDDQLAAVKLVMATLKIDDDALTPRNVLSRISHAKNHGKTPESIR
                     AEAIDKNGRLVADI"
     gene            complement(653..>1091)
                     /locus_tag="VII37_07595"
     CDS             complement(653..>1091)
                     /locus_tag="VII37_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069635.1"
                     /translation="MQVVFRNDSNESMKVSLDRIRLSFQVDEDHRQELQALTSGQVAD
                     EVLKPGGKDPSKSRSRIPLPVSIPHNNDKHWTELQQQVDAAGVPANVIAPHSTVQGLL
                     YFDLQGQFDLLNGAHLYVPDVVVMENNRSLTYFEIELSKPAGK"
BASE COUNT          256 a          317 c          259 g          259 t
ORIGIN      
        1 aaaatgtccg ctaccagccg cccgttttta tcgatggctt cggcgcgaat cgactccggc
       61 gttttgccat ggtttttggc gtgactaatg cggcttagca cattcctggg cgttaacgcg
      121 tcgtcatcaa tcttcagcgt cgccatcacc aacttcacgg ccgcaagctg atcatcgtcg
      181 tcgtaaatca cgaaatcccg cggcagcccg gcgctcactg cttctcgtcg caaaagccgc
      241 gcacacaaag agtgaaaggt agaaagccac ggcgaagacg gcggcactcc cgctcgcagc
      301 aacaggtccg atacgcgcgt ccgcatttcc tgcgcggcct tgttggtgaa agttaccgcc
      361 agtatggcct cagacggaac gccgttcgca atcaaattgg ccattcggaa agtgatcgcc
      421 cgggtcttgc cggtgcccgc gcctgccaga atgagcaggg ggccttctgt aatctctgcc
      481 gcgacacgct gttcgggatt taattcttcg agaagactta acatccgacc gttagtttca
      541 gaaatatttt gcacatctta tcgtagccga ccgtttcggt gcgcgcaaac gcagatatgt
      601 tgatgaaatt agccggcaaa aaattatttt cctgcgcgaa agaaaaacaa agctactttc
      661 cggcgggctt actcaattca atttcaaaat acgtcagcga gcggttattt tccatcacca
      721 caacgtccgg cacatacaag tgcgccccat tcaataaatc aaattgtccc tgaagatcaa
      781 agtacaaaag cccttgcact gtgctgtgtg gcgcaatcac attcgccgga acgcccgcgg
      841 catccacttg ctgttgcagt tccgtccagt gcttatcatt gttatgaggg atgctcaccg
      901 gaagcggtat cctcgaccgc gatttcgatg gatctttccc accgggctta agtacttcgt
      961 ccgccacttg gccggatgtc aaggcctgca attcctgccg atgatcctca tcaacctgga
     1021 acgatagacg aatacggtcc aggctaactt tcatgctttc gttcgaatca ttacgaaaca
     1081 ccacctgcat c
//
LOCUS       DATCBB010000684         1055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_346484, whole genome shotgun sequence.
ACCESSION   DATCBB010000684 DATCBB010000000
VERSION     DATCBB010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346484"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..874)
                     /locus_tag="VII37_07600"
     CDS             complement(join(114..547,547..874))
                     /locus_tag="VII37_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612353.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY5069636.1"
                     /translation="MRRNLFWLNDEQWRQIEPHLPRDVRGKQRVDDRRVISGIIHVLK
                     SGCRWCDCPPEYGPSTTIYNRFVRWAERGVWERLFCVLAARGRSTSTQMIDSTHIKAH
                     RSASGAKKGEQKQAIGRSRGGRNTKIHAIADAKGRLLSILLSGGEAHDCPPAQRLIRR
                     TKTAKKLLGDKAYDSAELRQWLKERGTRAVIPNRSNRKQPFRFDKKSYKQRHLIENAF
                     CRLKDFRRIATRYDRLARNFLASVCIAAAIVWWIL"
     gene            818..>1055
                     /locus_tag="VII37_07605"
     CDS             818..>1055
                     /locus_tag="VII37_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069637.1"
                     /translation="MRLDLPPLFIVQPKQIAPHLSLLRINRDSESATDSASNKLLGFG
                     PSFFPPTGNTQAAFGLMSERSRVLGWLRPRHHPNF"
BASE COUNT          213 a          296 c          295 g          251 t
ORIGIN      
        1 gcttcttcgt gtttttatgg gggaaacatg gacggaggca aacgtatatg gtccgtctga
       61 taaacctcat cttccaaaga aaacctatgg tttttttgaa ctagggccaa gactcataaa
      121 atccaccaca cgatagcagc ggcgatacag acggaggcaa ggaagtttct tgcgagccga
      181 tcatagcggg ttgcgatacg ccggaagtcc ttgagacggc aaaatgcgtt ctcgatgagg
      241 tggcgctgct tgtaggattt cttgtcgaag cggaacggct gtttgcggtt tgatctgttg
      301 ggaatgactg ctctggtgcc gcgctccttc agccattggc gcagttctgc gctgtcgtat
      361 gccttgtcgc cgagaagttt ctttgccgtc ttggtgcggc ggatcaggcg ctgggcaggc
      421 ggacaatcgt gtgcctcacc gccactcaac aggatggaaa gaagacggcc tttggcatct
      481 gcgattgcgt ggattttcgt atttcgcccg cctcgcgagc gaccgatcgc ctgcttctgt
      541 tccccctttt tgcgcccgag gccgagcggt gagctttgat gtgtgtcgag tctatcatct
      601 gtgtgcttgt tgatcggccg cgtgcggcaa gtacacagaa cagccgctcc cacacgccgc
      661 gctccgccca gcgcacaaaa cgattgtaga tcgtggtcga agggccgtat tccggcggac
      721 agtcgcacca gcggcaaccg ctcttcagca catggatgat gccgctgatc acgcgacgat
      781 cgtccacccg ctgcttgccg cgcacgtccc tgggtaaatg cggctcgatc tgccgccatt
      841 gttcatcgtt cagccaaaac agattgcgcc gcatttgtcc ctgctccgaa tcaacagaga
      901 cagcgaatca gcaaccgatt cagccagcaa caaattattg ggtttcggcc ctagcttctt
      961 cccgccaacg ggtaacaccc aagcggcatt tggtctcatg agcgagcgtt cacgagttct
     1021 cggatggctg cgaccacgtc atcatccgaa ttttg
//
LOCUS       DATCBB010000685         1036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_152856, whole genome shotgun sequence.
ACCESSION   DATCBB010000685 DATCBB010000000
VERSION     DATCBB010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152856"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VII37_07610"
     CDS             complement(<1..228)
                     /locus_tag="VII37_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069638.1"
                     /translation="MSHSRRNFIKYVVAGSVAAGCPIDASLLAVPDEKSSTTPRVEGE
                     HFEICHQVRDGHQFQRPEATRKADVVIVGGGV"
     gene            complement(242..>1036)
                     /locus_tag="VII37_07615"
     CDS             complement(242..>1036)
                     /locus_tag="VII37_07615"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069639.1"
                     /translation="ATAAKRGAPLLAAYSWLVFAFIYLPIVVLIVYSFNRDGVGGFPP
                     HHLTLNWYRQLFGDGAIWTSVLNSLIVAACSVALSLTLGLLAALALDRASFPGKLLFR
                     RLVLLPLILPGIITGLSLLMFAVYAGAHLSLLTVFLGHGTALISVATTELFAGLQKMD
                     RAQEEASLDLGATPLQTFWRVTLPNLKLSLIAAGLLIFTLSMDEIAVTFFLIGRDNTL
                     PLEIWGRLRRGITPEINAISTLIFAASVILIVIWFQLRTRAQRKTA"
BASE COUNT          255 a          279 c          317 g          185 t
ORIGIN      
        1 gacgccgccg ccgacaatca cgacgtcagc tttgcgtgtg gcctcggggc gctggaactg
       61 gtggccgtcg cgcacctggt gacaaatttc gaaatgctcg ccttcgacgc gcggcgtggt
      121 ggatgatttc tcgtccggaa cagccagcag gctcgcatca atggggcagc cggcggccac
      181 cgatcctgcc actacgtatt taatgaagtt gcggcgtgag tgcgacatgg agctcctctt
      241 ttcaagcggt cttgcgttgc gcacgtgtgc gcaactgaaa ccagatcaca atcaaaatca
      301 ccgaggcagc aaaaatcagt gtggagatgg cattgatttc cggggtgatt ccgcggcgca
      361 ggcggcccca gatttccagc ggcaaggtat tgtcgcggcc gatcagaaaa aaagtgacgg
      421 caatctcgtc catggagagg gtgaagatca gcaggcccgc cgcgattagt gaaagcttta
      481 agtttggcaa ggtcacgcgc cagaaagttt gcaacggcgt cgcgcccaga tcgagggatg
      541 cttcttcctg cgcgcgatcc attttttgca gcccggcgaa gagttcggtg gtggccactg
      601 agatcaatgc cgtgccatga cctaagaaaa ctgtaagcaa actcaagtgc gcaccggcgt
      661 agactgcgaa catcaacaag gaaagtccag tgatgatccc cggcaaaatc agtgggagca
      721 aaaccagccg gcggaaaagt aacttacccg gaaaactggc gcgatcaagc gctagcgcgg
      781 ctagcaggcc cagcgtgagc gaaagcgcga cgctgcaggc ggcaacgatc aggctattca
      841 gcaccgaagt ccagatggcg ccatcgccaa aaagttggcg gtaccagttc agtgtcaggt
      901 ggtgtggcgg aaaaccgcct acgccgtcgc gattaaacga ataaacgatg agcacgacga
      961 tcggtagata gatgaacgcg aacaccagcc atgaatacgc tgcaaggagg ggcgcgcctc
     1021 gctttgctgc ggtagc
//
LOCUS       DATCBB010000686         1256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_248508, whole genome shotgun sequence.
ACCESSION   DATCBB010000686 DATCBB010000000
VERSION     DATCBB010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248508"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1009)
                     /locus_tag="VII37_07620"
     CDS             complement(173..1009)
                     /locus_tag="VII37_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY5069640.1"
                     /translation="MTRLVQQIFAELRSTATVAIPRRLLLAISAAIFAAMFASAPIAA
                     ATQPRILVYTRNYTPDGKGYVHDNIATSVEAIQKMGAEKGFAVDYTDNPSVFSKTSLK
                     QYDAIVFSNSDNEAFSSDAQREAFKRYIESGGGFVGIHSAAGSERDWPYYTSVLGGKF
                     AAHPKIQPFTVRVADPQFPAVKGQPASFEWTDECYFLDHLNPDIHPVLVTDRTKLNYL
                     DALKIDVSSFPNPMPLAWFHEFDGGREFYIALGHHKEDYANPVLYGIIENGILWSMRH
                     KK"
BASE COUNT          271 a          304 c          399 g          282 t
ORIGIN      
        1 caattgcttg aaagaacgaa tcttgcgcgt ctcggcgcct gttttgaagg aaagcaaaga
       61 attagaaaat cgatttttcg gacgttggcg cgcgctaaac catagattta tctcgcgcac
      121 ttccccgttg aacacatcgg acgtgggcgg cgcactccgg accacggcaa aattacttct
      181 tatgcctcat agaccacaga atgccgtttt cgataatgcc gtagaggact ggattcgcgt
      241 agtcctcttt gtgatggcca agcgcgatgt agaattcgcg gccgccatca aactcgtgga
      301 accaagccag cggcattgga ttcgggaagc tggaaacatc aatcttcagc gcatccaaat
      361 agttgagttt ggtgcgatcg gtgaccagaa ccggatgaat gtcagggttg aggtgatcga
      421 ggaagtagca ctcgtcggtc cattcgaacg atgcgggctg gcctttcacc gccgggaatt
      481 gaggatcggc gacccgtacg gtgaagggct gaattttggg atgggccgca aattttccgc
      541 ccaacactga ggtgtagtag ggccaatcgc gctcggaacc agctgcggag tgaatgccga
      601 cgaagccgcc cccggattcg atgtagcgtt tgaaagcttc gcgttgagcg tcgcttgaaa
      661 aagcctcatt atcgctgttg gagaagacga tggcgtcata ctgcttgagg ctggtcttgc
      721 tgaagacgga tggattgtcg gtgtagtcaa ccgcgaaccc cttttccgcg cccattttct
      781 gtatcgcctc cacgctggtt gcgatgttgt cgtgcacgta tccttttccg tcaggcgtgt
      841 agttgcgcgt gtagacgagg atccggggct gggttgccgc agcgattggc gccgatgcaa
      901 acatcgccgc gaaaattgcc gcagagatcg caaggagaag tcgccgcgga atcgcgacag
      961 ttgcagttga gcgcagctcg gcaaaaatct gttgcaccag tctggtcata ttcttcctcc
     1021 tctttcagat ggacttttct tgccgacgtg attataggcg cggggcagga ggtgagcgaa
     1081 aacgcaggcg ggagtgatca gaaacgggag aggcactaaa ctggaggtat ggcggaattc
     1141 catgtcgagc attttgggtg cagggctgcg cgcgctgacg gtgaggcggt tggcggccag
     1201 ctgcgcgcgg cggggctcga cgaggggaat gctgcggcgg cagatgtggt ggtggt
//
LOCUS       DATCBB010000687         1026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_86724, whole genome shotgun sequence.
ACCESSION   DATCBB010000687 DATCBB010000000
VERSION     DATCBB010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86724"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VII37_07625"
     CDS             <1..519
                     /locus_tag="VII37_07625"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY5069641.1"
                     /translation="SQPWRNPRPAPQLSQMEGRVRMPRKYSLLMAWLPAVCWAGLIFS
                     LSTDAFSAHNTGKFLRPLVFWLMPHTTEAGFRTIHFVVRKAAHFTEYFVFCLLLYRGI
                     RGGRQGWRWTWALTALFISVGYSALDEIPQAFVASRTASPYDSLLDSIGAFFAMAVLV
                     LWFRARAVRTSG"
     gene            complement(610..>1026)
                     /gene="rplQ"
                     /locus_tag="VII37_07630"
     CDS             complement(610..>1026)
                     /gene="rplQ"
                     /locus_tag="VII37_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY5069642.1"
                     /translation="RHLKSGSKLGRNPSHRRATLRALVTNLFEHGRITTTLTRAKAAR
                     PVAEKIITLGKRDTLQSRRQAAAYIMLPAITKKLFSEIAPKFSDRAGGYTRIIHAGNR
                     VGDNAKVAILELVGYEFKKKEKKERPKKEEALDEKS"
BASE COUNT          167 a          254 c          341 g          264 t
ORIGIN      
        1 tcgcaaccgt ggcgcaatcc gcgccctgct cctcaattat cgcagatgga aggccgggtg
       61 cgcatgccgc gcaaatacag cctgttgatg gcctggctgc cggcggtgtg ctgggcaggg
      121 ctgatttttt cgctgtcgac agatgcgttt tccgcgcata acaccggaaa atttctaaga
      181 ccgctggtct tctggctgat gccgcacacg acggaagcgg gatttcgcac catacatttt
      241 gtggtgcgca aggccgcgca cttcacggaa tattttgtgt tttgcctgct gctgtatcgc
      301 ggtattcgtg gagggcgaca aggctggcgc tggacatggg cgctgacggc gttgtttata
      361 tccgtcggtt attccgcgct ggatgagatt ccccaggcat tcgtggccag ccggacggcg
      421 tcgccgtacg attccctgct ggattcaatt ggtgcgttct ttgcgatggc ggtgctggtg
      481 ttgtggtttc gcgctcgagc agttcgaaca tcaggatagg ttcggcgaaa gacgagttcg
      541 agaaacagaa aagcccgctt cggtggattg aggcgggctt ttcatttggg ccggaccaat
      601 atcgagcggc taactctttt cgtcgagggc ttcttctttc ttggggcgtt ctttcttttc
      661 tttctttttg aattcgtagc cgaccagttc gaggatggct actttggcgt tgtcgcctac
      721 gcggttgccg gcgtggatga tgcgggtata gccgccggcg cggtcggaaa acttgggcgc
      781 gatttcactg aacagtttct tggtgatcgc gggaagcatg atgtaagcgg cggcctggcg
      841 gcgcgattgc agcgtatcgc gtttgcctag agtgatgatt ttctcggcga ccggccgagc
      901 ggctttggcg cgcgtcagcg tggtggtgat gcggccgtgt tcgaacagat tggtaaccag
      961 ggcgcgcaaa gtggcgcggc gatgggacgg gttgcgtccg agcttcgatc ctgacttgag
     1021 atggcg
//
LOCUS       DATCBB010000688         3025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_120279, whole genome shotgun sequence.
ACCESSION   DATCBB010000688 DATCBB010000000
VERSION     DATCBB010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120279"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VII37_07635"
     CDS             <1..1313
                     /locus_tag="VII37_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5069643.1"
                     /translation="ALAGAARIILTAAFARELGAKKFGIALAAALAATPAVWYVNDHQ
                     FAMNAFEPLFWTGCAFAVARMIYTNNSKWWVVFGAIAGVGLENKYSIAAFAFSLLTGL
                     LLTPQRRLLFSPWILAGGVAALLLFLPNLLWNVKHHWPFLELMRNIRASGRDVVSSPS
                     AFLAQQILIMTPATLPFWLGGLWFYFSDAGKKLRVFGWTFIFTIGFFLLAHGKNYYPA
                     PAYPLVLAAGAVASENFLGKRHFEERWREGAAVKVAIFGWLAVGILLLLPVVLPVLPI
                     SAYLRYQAHLPFAVPKSEHNQDTAILPQHYADEFGWEDMVRRVASFYHSLSPEEQSRT
                     AIFASNYGEAGAVDFFGSRYGLPTAISTHQNYFLWGPRNYDGEIVIRIGTKLEDARES
                     YESVVVAATLDNPYTMPHEKNPILLCRHRKKNLHADWPDIKNWD"
     gene            1377..2159
                     /locus_tag="VII37_07640"
     CDS             1377..2159
                     /locus_tag="VII37_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5069644.1"
                     /translation="MILQAQTALITGSGRGIGRAIASLFASEGASVFLCARSHDQLAS
                     AAAEISRGGGQVAFATADVTREDDCRRVVDAAREKFGPVTILVNNAGHYGPVVPVEEY
                     PLADFDEVIAVHLRAAFLLTQLVLPEMYEHEHGVILNISSLSAKAAFSWGSAYAAAKA
                     GMLGLTRVTAAEAARKGVRVNAICPGPVTETVMSQELGATLAKKIGVSSEEQLAGFLQ
                     SILQGRGQTADEIANAALFLCSKASSAMTGQSINVDGGAAFF"
     gene            complement(2205..>3025)
                     /locus_tag="VII37_07645"
     CDS             complement(2205..>3025)
                     /locus_tag="VII37_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5069645.1"
                     /translation="GPRMQVATRAMTPTGMYPLLGYSWELKVPTGVQYVDGVDGARKA
                     VREQVMYGADWIKYYSDRKYHFEADNVLHSMVNFTDEEAKAIVDESHRLGKRVAAHAI
                     GSDGIAAALRAGVDTIEHGDGLTDALLDEMAKKGVYWVPTITVGAYVAPGRGGNWTRM
                     VDLERVAFQKALKKNVKIVFGTDAGGFDWKELNQAKEFEYYVQYGMTPMQAIRSATSM
                     AGELLGWKDKVGTIEAGKWADMVAVSGDPLKDISELSRVKFVMKAGTVYKNELH"
BASE COUNT          613 a          849 c          797 g          766 t
ORIGIN      
        1 ctgcattggc gggggctgct cgcatcattt tgacggcggc tttcgcgcgc gagcttggag
       61 caaagaaatt tggcatcgcg ctcgctgcag cattggctgc tacgcctgcg gtctggtatg
      121 tcaacgacca ccagttcgct atgaatgcct tcgagccgct gttctggact ggctgcgcgt
      181 ttgccgtggc ccgaatgatc tatacgaaca attctaaatg gtgggtcgtc ttcggtgcca
      241 tcgctggagt cggcctggaa aataaatatt ccatcgccgc atttgcgttt tctctgctta
      301 ctgggctgct actgacaccg cagcgccgac tcttgttttc tccgtggatt ctcgccgggg
      361 gcgtcgccgc tctattatta tttcttccta acctgctttg gaatgttaag caccattggc
      421 cgtttctgga gttgatgcgc aacattcgcg cgtccggtcg cgatgtggtt tcgtcgccgt
      481 cagctttcct ggcacaacaa attctgatca tgactccggc gacgcttccc ttctggttgg
      541 gcgggttgtg gttttatttt tctgatgccg gtaagaaatt gcgcgtcttt ggctggacct
      601 ttattttcac gataggcttt ttcttgctcg cgcacgggaa gaattattat cccgcgccgg
      661 cctacccatt agttcttgcc gccggagccg tcgccagtga aaatttctta ggcaaaagac
      721 atttcgaaga gcgctggcgg gagggggcag cagtaaaagt ggcgatattc ggctggctcg
      781 ctgtcgggat actccttttg ttgccggttg tgcttcccgt tttaccgatc agtgcgtacc
      841 tgcgttatca agcgcatctg ccattcgctg ttccaaaatc cgagcataac caggacacgg
      901 caatcttgcc acagcattac gccgatgagt tcggctggga agatatggtc cgccgcgtag
      961 caagctttta tcattcgctc tccccagagg aacaatccag aaccgcgatt ttcgccagca
     1021 actatggtga agccggcgct gtggactttt tcggcagcag gtacggtttg ccgacagcga
     1081 taagcacgca tcagaattat tttttgtggg gaccacgcaa ttatgacgga gaaatcgtca
     1141 ttcgaattgg aaccaaactt gaggatgcgc gtgaatccta tgagagcgtc gttgtagcag
     1201 ccacgctgga caacccgtac accatgcccc acgaaaagaa cccgatctta ctttgccggc
     1261 accgcaagaa aaatctccat gcagattggc ctgacatcaa gaattgggac taatccaact
     1321 gtggacataa ccaagcgact tgcatgcgct ccctggtcct tctattctcc ttccctgtga
     1381 ttctccaagc gcaaacggct ttgatcaccg gctcaggccg cggtattggc cgagccatcg
     1441 catcgctatt cgccagtgaa ggagcatccg tttttctttg cgctcgctcg catgatcaac
     1501 tggcttcagc tgcggcggag atctctcgcg gcggcggtca agttgcattt gcaaccgccg
     1561 atgtaacccg tgaagacgat tgccgacgag tggtcgacgc ggcgcgcgaa aaatttggtc
     1621 cggttactat tcttgtgaat aacgcgggcc actacgggcc ggttgttccg gtggaagaat
     1681 acccgctcgc tgattttgat gaggtgatcg cggtgcatct gcgcgccgct tttctgctta
     1741 cacaattggt attgccagaa atgtacgagc atgagcacgg agtcattctc aatatttcca
     1801 gtttgtcggc caaggccgct ttttcgtggg gttctgctta tgctgccgcc aaagccggca
     1861 tgctgggctt gacgcgggta accgcggccg aggcggcgcg caaaggcgtt cgcgtgaacg
     1921 ccatttgtcc ggggccagtt accgagacgg tgatgtccca ggagctgggc gcaacactgg
     1981 cgaaaaaaat tggtgtgagt tcggaggagc aacttgccgg cttcctgcaa tcgattttgc
     2041 agggccgcgg ccaaactgcc gacgagatcg cgaacgctgc gctttttctg tgctccaaag
     2101 cttccagcgc catgacgggt cagtccatca acgtagacgg tggcgccgca ttcttctgat
     2161 gggcgcctat ctggtgagga aagcgcctca atatacgatc gcacttagtg cagttcgttc
     2221 ttgtagaccg ttccggcctt catgacgaat ttcacgcgcg ataattctga aatatctttc
     2281 aagggatcac cgctcacggc aaccatgtcc gcccatttgc cagcctcgat cgttccaact
     2341 ttatctttcc agcccaacag ttcaccggcc atcgaagttg ccgatcgaat ggcctgcatg
     2401 ggagtcattc catactgcac gtagtattca aactccttgg cttgatttaa ttctttccaa
     2461 tcgaagccgc cggcgtccgt accaaaaaca attttcacgt ttttcttcaa tgccttttga
     2521 aaggcaacgc gttccaggtc caccatccgg gtccagtttc caccgcgtcc gggtgcaacg
     2581 taagcgccca cggtgatcgt aggtacccag tagacgccct tttttgccat ttcatccagc
     2641 aaagcatcgg taagtccgtc gccgtgctcg atggtatcga cgccggcacg caacgctgcg
     2701 gcgattccgt ccgaaccaat cgcgtgggcc gcaactcgtt tacccagccg gtgcgattcg
     2761 tcaacgatgg ctttggcttc ttcgtcggta aaattcacca tgctgtgcag cacgttatca
     2821 gcttcaaaat gatatttgcg atccgaataa tatttgatcc agtcggcgcc gtacatcacc
     2881 tgctcgcgta cagccttgcg cgcgccatcc acgccatcga catattgcac accagtgggc
     2941 actttcagtt cccacgaata tcccaacaat ggatacatgc cagtgggagt catcgcccga
     3001 gtggccactt gcatgcgcgg cccgg
//
LOCUS       DATCBB010000689         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_217319, whole genome shotgun sequence.
ACCESSION   DATCBB010000689 DATCBB010000000
VERSION     DATCBB010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217319"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="eno"
                     /locus_tag="VII37_07650"
     CDS             complement(<1..1124)
                     /gene="eno"
                     /locus_tag="VII37_07650"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY5069646.1"
                     /translation="MSTKITRVHARQVIDSRGNPTVEADVTLTSGAVGRAAVPSGAST
                     GEHEALELRDGDKSRYLGKGVLKAVANANGEIAKAVTGLDAADQRALDHKMISLDATP
                     TKSRLGANAILAVSMAAARAASIALKQPLYKYLSRYSTDSSANLLPVPMMNILNGGAH
                     ADSSVDFQEFMVMPVGAPNFSEALRWGVEIFHALKAALKKRGYSTAVGDEGGFAPSCK
                     SNEEAIQIVLEAITAAGYKPGEQVRIALDPASSEFYDKASGKYIFKKSDKSQHSSSEM
                     AAYWTSWVEKYPIVSIEDGMAEDDWAGWKELTESVGRKGAGKKSIQLVGDDLFVTNTE
                     RLGRGITEGVANAILIKLNQIGSVTETIDAIELARKAGYNS"
     gene            1504..>2049
                     /locus_tag="VII37_07655"
     CDS             1504..>2049
                     /locus_tag="VII37_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5069647.1"
                     /translation="MADTTGVRDSVLQAIGNTPVVRLRKLVSSSMGEVLVKLEYYNPT
                     GSYKDRMALAMIEGAEARGELRPGMRVVEWTGGSTGSSLAMVCAIKGYRFTPISSDGF
                     SDEKLRTMRLFGGDLEIFPAEGGKLAPELYQKMKRRAEELAKEPGTFYTNQFNNTDAL
                     RAYMGIGKELAEQAGTGLSAFC"
BASE COUNT          423 a          513 c          641 g          472 t
ORIGIN      
        1 gagttgtagc cggctttgcg ggctagttcg atggcgtcga tggtctcggt aacgcttcca
       61 atttgattca gcttgatcag aatggcgttg gcaacacctt cggtgatgcc gcggccgagg
      121 cgttctgtgt tggtgacaaa gagatcgtcg ccgaccagtt ggatggattt tttcccggcg
      181 ccctttcggc cgacactttc tgtaagttct ttccagccgg cccagtcgtc ttcggccatg
      241 ccgtcttcga tgctgacgat gggatacttt tcgacccaac tggtccagta cgcggccatc
      301 tcgctgctgc tatgttggga tttgtcggac ttcttgaaaa tgtatttgcc gctggctttg
      361 tcgtaaaact cgctcgatgc gggatccagt gcgatgcgta cttgctctcc cggtttgtag
      421 cctgcggcgg tgatggcctc gagcacgatc tggatggcct cttcgttgga cttgcagctt
      481 ggagcgaaac cgccttcgtc gccgactgcc gtggagtagc cgcgtttctt tagagctgct
      541 ttcagcgcgt ggaaaatttc cacgccccag cgcagggctt ccgaaaaatt cggggctccg
      601 acgggcatga ccatgaattc ctggaagtcc accgagctat cggcgtgggc gccgccgtta
      661 agaatattca tcatgggtac gggtaagagg tttgcggagg aatccgtgga atagcgcgaa
      721 agatatttgt acagcggctg cttcagtgcg atggaggcgg cgcgcgcggc ggccatggac
      781 acggccagaa tagcgttggc gcccaaccgc gatttggtgg gcgtggcatc cagcgagatc
      841 attttatgat ctagggcgcg ctggtcggcg gcatcgaggc cggtcaccgc tttggcaatt
      901 tcgccgttgg cgttcgcgac tgctttcaag actcctttgc cgaggtagcg cgatttgtcg
      961 ccatcacgca attccagcgc ttcgtgttcg ccggtggatg cgccagacgg aaccgccgcg
     1021 cggcctaccg cgcccgacgt cagcgttaca tccgcttcca cagtgggatt cccgcgggaa
     1081 tcgatgacct ggcgggcgtg tactcgagta attttcgtgg acattattga gatgaatctc
     1141 cttggagtga ataaagattt tacagaaaag cgcgaaaaag agcacgctgg gcgacagtcg
     1201 gttgtgtcct aatctcaggg caaagctgcg agaactccca gatccaatct gaaacgccag
     1261 ctaccattac cggaatgaac tcggcaaagg tcgtagcatg cgaaaatata gccgaacaaa
     1321 ttgactgtgg taggcattgc gggtcatccc cgttgttgat ctccggcctt tgacggggct
     1381 ttgttttaag accgtaaaaa ttcagcaagc gcttgctatt tgccaagtac tgtcgcgaaa
     1441 actggagcct tgccatagtc gaatctacaa gcatatgctg ctgcggaatt caggagaaga
     1501 atcgtggcgg ataccacggg agttcgagat tccgttttac aggcgattgg gaatacgccg
     1561 gtggtgcggc tgcggaagtt ggtgtcatct tcgatgggcg aggtgctggt aaagctggag
     1621 tattacaatc ccaccggttc ttacaaagat cgcatggcgc tggcgatgat tgaaggggcg
     1681 gaggcgcgcg gagagttgcg accaggcatg cgtgtggtgg agtggacggg agggagcacg
     1741 ggatcgtcgc tggcgatggt gtgcgcgatc aagggatacc gcttcacgcc catttcttcg
     1801 gacggatttt cagacgagaa gctgcggacg atgcgccttt tcggcggaga cctggaaatt
     1861 tttccggcgg aaggaggcaa gctggcacca gagctttacc agaagatgaa aaggcgcgcg
     1921 gaagaactgg caaaagagcc gggcacgttc tatacaaacc aattcaacaa cacggatgcg
     1981 ctgcgggcgt acatgggcat cggcaaggaa ctggcggaac aagcgggcac cggtttgtcg
     2041 gcgttttgc
//
LOCUS       DATCBB010000690         1385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_55255, whole genome shotgun sequence.
ACCESSION   DATCBB010000690 DATCBB010000000
VERSION     DATCBB010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55255"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="ruvB"
                     /locus_tag="VII37_07660"
     CDS             complement(<1..890)
                     /gene="ruvB"
                     /locus_tag="VII37_07660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY5069648.1"
                     /translation="MPDGTAKRVIGKTAERIVSAQAFDEDARIEASVRPKRLDEYIGQ
                     KRVKENIQIAIEAARSRGEALDHVLLYGPPGLGKTTLAQIIANELQVPIKTSAGPLLE
                     RKGDLTAILTSLETKEVFFLDEIHRLQPAVEEVLYPAMEDFRVDLIIGQGPGARIHPF
                     QLGHFTLIGATTRAGLITAPLRSRFGIVHRLDFYEPADLLIIIRRSAKILNIEMDEAG
                     AEEIARRCRGTPRIANRLLRRARDFAEVRAAGRITQAVAQEALAMLGVDEQGLDEVDR
                     NLMLALLDKYGGGPVGFGTLA"
     gene            complement(892..>1385)
                     /gene="ruvA"
                     /locus_tag="VII37_07665"
     CDS             complement(892..>1385)
                     /gene="ruvA"
                     /locus_tag="VII37_07665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY5069649.1"
                     /translation="EVTLLIHTHVREDALSLYGFVSSREKHLFEMLLSASGVGPSLAL
                     KILSGMSVDELVPAIRGSDLGRLTQIPGVGRKTAERMVVELKDKLDAVIVESQKPASS
                     SPAGVEADVVSALVNLGYDARVAEKTVEEARKESGSANFEKLLRAALQALSQSKGRAA
                     RTS"
BASE COUNT          277 a          452 c          343 g          313 t
ORIGIN      
        1 gccagtgtgc cgaaacccac cggcccgccg ccatatttat cgagcagcgc cagcatcaag
       61 ttgcggtcca cttcatcgag tccctgttca tctacaccaa gcattgcgag cgcttcctgc
      121 gctaccgcct gggtgatgcg tcccgccgcg cgcacttccg cgaagtcgcg cgcgcgccgc
      181 aacaatcggt tggcgattcg cggtgttccg cggcaccgtc ttgcaatctc ctccgcgcca
      241 gcctcgtcca tctcgatatt cagaattttt gccgagcgcc gaataataat cagcaaatcc
      301 gcgggctcgt aaaaatccag ccggtgaaca attccaaaac gcgaacgcag cggcgcggta
      361 atcaatcccg cgcgcgtggt ggccccgatc aaggtgaagt gccccaactg aaatggatgg
      421 atgcgcgcgc ccggcccctg gccgataatc aaatcgacgc gaaaatcttc catcgccgga
      481 tacagcactt cttctactgc cggttgcaag cgatgaattt cgtcgagaaa aaacacttcc
      541 ttcgtttcta gcgaagtaag aattgccgtc aaatcccctt tgcgctccag caatggcccc
      601 gcgctggtct tgatcggcac ctgcagctcg ttggcaataa tctgcgccag cgtggtcttc
      661 cccaaccctg gcgggccgta caacagcacg tgatcgagtg cttcaccgcg gctgcgcgcc
      721 gcctcgatgg caatctgaat attttctttg acgcgttttt gcccgatgta ttcatccagc
      781 cgtttgggcc gcacactggc ttcaatgcgc gcgtcttcat cgaaggcttg cgccgacacg
      841 atgcgctccg ccgtcttgcc gatcactcgt tttgcggttc cgtctggcat tttaggaagt
      901 cctggcagct cgacccttgc tctggcttag cgcttgcaac gcagcgcgca acaatttttc
      961 aaaattcgct gacccggatt ccttacgcgc ttcctcaacc gttttttccg cgacgcgcgc
     1021 gtcgtagccc agattcacca gcgctgaaac tacatcggcc tccacacccg caggcgaact
     1081 cgacgctggt ttttgcgatt ccacgattac cgcgtccagc ttatctttta gttccacgac
     1141 catgcgctcg gccgtttttc tacccacgcc cggtatttgc gtcaatcgtc ccagatcgct
     1201 tccgcggatc gctggcacca attcgtccac gctcatgccc gacaaaattt tcagcgccag
     1261 gctcggaccg acgccagacg cggaaagcaa catttcaaaa aggtgttttt cgcgtgagga
     1321 aacaaatcca tacaacgata gcgcgtcttc gcgcacgtgg gtatgaatca gcagggtcac
     1381 ttcgg
//
LOCUS       DATCBB010000691         1221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_443506, whole genome shotgun sequence.
ACCESSION   DATCBB010000691 DATCBB010000000
VERSION     DATCBB010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443506"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VII37_07670"
     CDS             <1..739
                     /locus_tag="VII37_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069650.1"
                     /translation="AQACFLAAICIFSSQPFQPLLRAQDHPMPAGHEGMVMDDSAPEP
                     SPAKLLADKRESEFNHHLAGFFVVLAGIFILAEGRLSARWPAVRYAWPLCFLLAGIFV
                     FVFSDTELWPFGRQSWLFGLSHHAEVRQHKTFAAILLALGVIEVLRVRGTLKAAWSAW
                     VFPVLAIAGSIILIFHEHGGGMHGANHMEVMARIQHQHMSYLAAGLGIGLTKGLSEIK
                     TGWQGLFAKLWPSIMIVLGVLLMFYVE"
     gene            complement(757..>1221)
                     /locus_tag="VII37_07675"
     CDS             complement(757..>1221)
                     /locus_tag="VII37_07675"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5069651.1"
                     /translation="WGKQKSGNGFGMGVAMHRSFLTYVATVVRVQVDDAGHVQIKRVD
                     TALDAGLVVNPEMARQQFEGAAVMGTSLAFYGEITASNGAIEQSNFNDFQMARMKDAP
                     RETYVHLVASDAPPAGIGEPGLPPFAPALCNAIFAATGKRIRELPLSRAGFA"
BASE COUNT          215 a          385 c          339 g          282 t
ORIGIN      
        1 cgcgcaggcg tgcttcctgg ctgcgatttg cattttttca tcccagccat ttcagccatt
       61 gttgcgtgcg caggaccatc ccatgcccgc gggccacgaa ggcatggtga tggacgattc
      121 cgctcccgaa ccatcacccg ccaagctgct tgccgataag cgcgaaagcg aattcaatca
      181 tcacctcgcg ggattctttg tcgtgctcgc cggcattttt attcttgccg aaggccggct
      241 cagcgcccgc tggccggccg ttcgctatgc ctggccgcta tgttttttgc tggcgggaat
      301 ttttgtattc gttttcagcg acaccgagct gtggcccttc ggccgccagt cctggctctt
      361 cggattgtca catcacgcag aagttcggca gcacaaaaca ttcgccgcga tcctgctcgc
      421 cctcggagtg atcgaagtgc ttcgcgttcg tggcactcta aaggccgcgt ggtccgcctg
      481 ggtatttcct gtcctggcga ttgccggctc catcatcctg attttccacg agcatggcgg
      541 cggtatgcac ggcgcgaatc acatggaagt catggcgcgc atccagcatc agcatatgag
      601 ctacttggcg gcaggattag gaattgggct gaccaagggt ttgtcggaaa tcaaaaccgg
      661 ctggcaggga ctttttgcca aactctggcc gagcatcatg attgttctcg gcgtgctgct
      721 gatgttctac gtggagtagc gccgccgccg aaaatctcag gcgaatcccg cgcggctcaa
      781 gggcagttcc cgtatgcgct ttccggtggc cgcaaaaatc gcgttgcaca gcgccggcgc
      841 gaatggcggc agccccggct caccgattcc cgcgggaggc gcgtcgctcg ccaccaggtg
      901 cacgtaagtt tcgcgcggcg catccttcat ccgcgccatc tggaaatcgt taaaatttga
      961 ttgctcgatg gcgccattgc tggcggtgat ctcgccgtaa aacgccaggc tggtgcccat
     1021 caccgccgcg ccttcaaact gttgccgcgc catttccggg ttcaccacga gtcccgcgtc
     1081 gagcgcggtg tccacacgct ttatttgcac gtggcccgcg tcatccacct gcactcgcac
     1141 cacggttgcg acataggtca aaaaactgcg atgcatcgct acgcccatgc caaagccatt
     1201 gcctgatttt tgctttcccc a
//
LOCUS       DATCBB010000692         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_314120, whole genome shotgun sequence.
ACCESSION   DATCBB010000692 DATCBB010000000
VERSION     DATCBB010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314120"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VII37_07680"
     CDS             complement(<1..960)
                     /locus_tag="VII37_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069652.1"
                     /translation="MSALRRFFRRLFNTAAMRAQEERLREEIAEHIALQTEENLRAGL
                     SAVEARRQAMLKFGGVEAMKQDYRAERGLVLIENLLGDLRNAVRAISRMPGLATVIVL
                     SLAIGIGVNTTILSWIQLILLDPLPAVSGASKFVLVEPHTETDGYPGASWLEYRDLQT
                     QLPALRDVVASEMVPFNVGEKGKTERTHGQFISGNFFSALGLKPASGRFIRPEEAERS
                     GTEPVVVISYDYWQTRFRGTAEAVGQKLRVNDRDLVVIGVAPREFQGTLLPLKFELWV
                     PATMAPALLGGTRDLEDRNSRGFSLIGMLKPGATREEAQTEFSA"
     gene            complement(957..1304)
                     /locus_tag="VII37_07685"
     CDS             complement(957..1304)
                     /locus_tag="VII37_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5069653.1"
                     /translation="MKQKTDVLQGTLALMVLKTLDVLGPLHGYGIARRIEQISGDLLA
                     VNQGTLYPVLLRLEHEGAIASEWGASENNRRARFYRLTREGRKQLQVERQDWEQTAAI
                     IGRFFDVKTGDLS"
     gene            complement(1417..>2027)
                     /locus_tag="VII37_07690"
     CDS             complement(1417..>2027)
                     /locus_tag="VII37_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEY5069654.1"
                     /translation="YIQLGNVAGEPQCWWFWERAGFSSTDLSRMFDEGHVRLRNEQLP
                     ELEIIAAGTRKNLKLPSRARLVVVPMLVAHAATHGQQADNVIDMDTAPVGDLLAAPEA
                     WCPNPGQTTCLTVRGDAMAPLINEGDIVAVDGAQTDPKELSGKIVVTWQPQRGLMLSR
                     FLMVNGTQMLESENLSYQPVPQLKNRNWRIVGRVLWWIRKAS"
BASE COUNT          450 a          686 c          459 g          432 t
ORIGIN      
        1 ggcactaaat tcggtctgcg cttcctcgcg cgtcgcgcca ggcttcaaca tgccgatcaa
       61 cgaaaacccg cggctgttgc gatcttccag atcgcgtgtt cccccaagca acgcgggcgc
      121 catcgtggcc ggcacccaca gctcaaactt caacggcagc aatgttccct ggaattctct
      181 gggtgccacg ccgatgacca caaggtcgcg atcattcacg cgcagttttt gccctacagc
      241 ctcagccgtg ccgcgaaacc gcgtttgcca gtaatcatac gaaatcacca cgaccggctc
      301 ggttccgctc cgttctgcct cctccggtcg aataaatcgt ccgcttgcag gcttcaaacc
      361 cagcgcagaa aaaaaattgc ctgagatgaa ttgtccgtgc gtccgctcag tctttccctt
      421 ctctcccacg ttgaatggca ccatctccga cgccaccacg tcccgcagcg ccggcaattg
      481 cgtttgcaag tcgcggtatt ccaaccacga agccccggga tacccgtccg tctccgtgtg
      541 cggttccacc agcacaaact tcgatgcccc actcacggcc ggcaacggat ctaaaaggat
      601 cagctgtatc caggaaagaa tcgtcgtgtt aacgccaatt ccaatggcca gcgaaagaac
      661 aatcaccgtc gcaagcccgg gcatgcgcga aatcgctcgc accgcgtttc gcaaatcccc
      721 cagcagattc tcaatcaaca ccagtccgcg ctccgctcga taatcttgtt tcattgcctc
      781 cacgccgcca aacttaagca tcgcctgccg tcgtgcctcc acggctgata acccggctcg
      841 aagattttct tccgtttgca gagcaatatg ttctgcaatc tcttcccgca accgttcttc
      901 ctgcgcccgc atcgccgccg tgttgaacag ccgcctaaaa aaccgccgca gagcgctcac
      961 gacaaatccc ccgtcttcac atcgaagaac cgcccgataa tcgccgccgt ttgctcccaa
     1021 tcttgtcgct caacctgtaa ctgcttccgc ccttcccggg tcaatcgata aaaccgcgcc
     1081 ctgcgattat tctccgaggc cccccactcc gaagcgattg cgccctcatg ctccagcctc
     1141 aacagcactg gatacagcgt gccctgattc accgccagca aatccccact gatctgctca
     1201 atgcgccgcg caatcccata cccatgcaac ggccccagca catccagcgt cttcaaaacc
     1261 atcaacgcca acgtcccctg cagcacatcg gtcttttgtt tcatgacaac acctgtttca
     1321 tcccacgcgc catgttgttt tccaacagga gactgccaca cttcctttgg gaaagcaaca
     1381 ggtaagcgca attcactggt tcaaaagcac tggaaatcag ctagcttttc gaatccacca
     1441 cagcacgcga ccaacgatcc gccaattgcg gtttttcagc tgcggcaccg gctggtagga
     1501 gaggttctcg gactccagca tctgtgtacc attcaccatt aagaatcgcg agagcatcag
     1561 gccacgttgt ggttgccagg tcacaacgat tttaccgctc aattctttag gatccgtctg
     1621 cgcaccatcg acggcgacga tgtcgccttc gttgatcagc ggagccatcg catcgccgcg
     1681 gactgttagg caagtggttt gacccgggtt cggacaccac gcctctggag cagcaagcaa
     1741 atcgccgacc ggcgcagtgt ccatatctat tacattgtcg gcttgttggc cgtgggtggc
     1801 ggcgtgagcg actaacatag gaactaccac aagacgcgca cgcgagggta gtttgagatt
     1861 ttttctggta ccagcggcta tgatttccaa ttccggaagt tgctcattac gcaacctgac
     1921 gtgcccttca tcgaacatac gagaaagatc cgtgctgctg aatcccgcgc gctcccaaaa
     1981 ccaccagcat tgcggttccc cggccacgtt gcccaactga atgtaac
//
LOCUS       DATCBB010000693         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_86951, whole genome shotgun sequence.
ACCESSION   DATCBB010000693 DATCBB010000000
VERSION     DATCBB010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86951"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VII37_07695"
     CDS             <1..902
                     /locus_tag="VII37_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY5069655.1"
                     /translation="NPESGFIVTANARVTGPDYKPFLTDRWEEPYRTARIFDLLQGRH
                     DLRPEDMLKVQNDTYTYPHMFLAEQLSAAAKSVPPKDARAQRLIQQLKDWNGIANADS
                     VEVSFLQAVRRSLMEMILAPYLGNDTDLYEWRSMAFLQKVLTERPAKWLPPTCKSYDE
                     LLTSAADRAVSKLAAQTNSQRPTDWPWKRFNSLQMLHPMGQTGILKLLFSISDKPQSG
                     TVYSVRAASKRHGPSERFVANPANWDDSILLITSGESGQLGSSHYTDQFSFWYEGKPI
                     VLPFSDAAEKSTRKHTLTLKPGP"
     gene            1104..1376
                     /locus_tag="VII37_07700"
     CDS             1104..1376
                     /locus_tag="VII37_07700"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY5069656.1"
                     /translation="MNSPVPKKPDTPKKPDFERSLARLEEVVRRLESPQLSLDDAMQL
                     FEEGVALSRECQKQLEEAEGRVEILLKKADGKLAPEPFEPETENES"
     gene            1414..>2241
                     /locus_tag="VII37_07705"
     CDS             1414..>2241
                     /locus_tag="VII37_07705"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY5069657.1"
                     /translation="MNLPDFFEQDRLAVDAALDRLLPPESTEPVSIHTAMRYSVFAGG
                     KRIRPILCLEAARIFEAEAQADASGNPESSPAMHAACAIEFIHTYSLIHDDLPALDND
                     HLRRGKPTCHKKFGEATAILAGDGLLTLAFQIIGESSTSAERTLAILQEVSSAAGTVK
                     GMVGGQVADLESEGKRVGPQTLEYIHRSKTAALIRASVSAGAISAGAGIADVARLRNF
                     GETIGWAFQVTDDILDVEESSSALGKTAGKDIAQQKATYPAVFGVAQSHEIAAKLAND
                     "
BASE COUNT          548 a          646 c          569 g          478 t
ORIGIN      
        1 tcaatccgga aagtggcttt attgtcaccg ccaatgcacg ggtcaccggc ccagattaca
       61 aaccgttctt gaccgatcgg tgggaagagc cgtaccgcac cgcaagaatt tttgatttgc
      121 tgcaaggccg gcacgatttg cgtccagaag acatgctcaa ggtgcagaac gacacctaca
      181 cctatccgca catgttcctg gccgaacaat tatccgcagc tgccaaaagc gtgccgccga
      241 aggatgcacg tgcccaacgc ctaattcagc aattaaagga ctggaatggc attgccaacg
      301 cggattcggt agaagtttct ttcttgcaag ccgtgcggcg ctcgctgatg gaaatgattc
      361 ttgcgcctta tctcggcaac gatactgacc tgtacgagtg gcgcagcatg gcgtttctgc
      421 aaaaagtttt aaccgagcgc cccgccaagt ggcttcctcc cacgtgcaaa agttacgacg
      481 aactcctgac atcggcggcg gatcgagcgg tttcaaaact cgcggcgcaa acgaacagtc
      541 agcgccccac ggattggcct tggaagcgat ttaattccct tcaaatgctg catcccatgg
      601 gacaaacggg aattcttaag ttgctgttca gcatcagcga caaaccgcag tctggaacgg
      661 tttacagtgt acgtgccgca agcaagcgcc atggcccgtc ggagcgcttc gtcgccaatc
      721 ctgccaactg ggatgactca atactgttaa tcacctctgg agaatccgga cagctaggaa
      781 gttcccacta caccgatcag ttttcctttt ggtatgaagg aaaaccgatt gtgctgccct
      841 ttagcgatgc agctgaaaaa tccacgcgga aacacacttt aacgttaaag cccggaccat
      901 aactcgcgag acgaatagct gcagacaatt tgatttgcga tagcggtcgc gcctatcacc
      961 aattaaaatc gtcgtcgcac cataaagcga ttgtgtcctt tctcgcgcct gccttagaat
     1021 gctcacatgt tgtcaccacg acgatttcag atggctggcg ttaattaccc gcaatcttgc
     1081 aatgctaaag acaaggctga cgcgtgaatt ctcctgtacc taaaaagccg gacaccccta
     1141 agaaaccaga ttttgagcgt tcgctcgcgc gcctggaaga agtagttcgc cgcctcgaga
     1201 gcccacaact ctctctcgac gacgccatgc agctttttga agaaggcgtg gctctttctc
     1261 gcgagtgcca gaaacaactg gaagaagccg aagggcgtgt tgaaattctg ctgaagaagg
     1321 ccgacggaaa actcgcaccc gagccgtttg aacccgaaac cgagaacgaa tcttgaagtt
     1381 gcacatactg ccccctctgt tcgtgcccac gcgatgaacc tccccgattt ctttgagcaa
     1441 gatcggctgg ccgttgacgc cgcgctggac cgcttgcttc cacctgaaag cacagagccg
     1501 gtttccatcc ataccgccat gcgctacagc gttttcgccg gcggcaaacg catccgccct
     1561 attctgtgtc tggaagccgc ccgtattttt gaggcggaag cccaagctga cgcgtcaggc
     1621 aatcctgagt cttccccggc catgcatgct gcctgcgcaa ttgaattcat ccatacctat
     1681 tccttaattc acgacgatct gcccgcgctc gataatgacc atctccgccg cggtaaacct
     1741 acttgccaca aaaaattcgg cgaggctacc gcaatcctgg ccggagatgg attgctcacc
     1801 ctggcatttc aaatcatcgg cgaaagttcg acgagtgctg agcgcactct cgcaattctg
     1861 caggaagtaa gcagcgctgc aggcaccgtg aaaggtatgg ttggcggaca agttgcggac
     1921 ctcgaaagcg aaggcaagcg cgtcgggccg caaacgctcg agtacattca tcgttcgaag
     1981 accgccgctc taattcgggc atctgtgagc gccggagcaa tcagcgccgg cgcaggaatt
     2041 gcggatgtag cccgacttcg taattttggc gaaacgatcg gctgggcctt tcaggtaacc
     2101 gacgacattc tggatgtgga agaatcatcc tcggcgctcg gcaaaactgc cggcaaagac
     2161 atcgcccaac aaaaggctac ctatccggcg gtgtttggtg ttgcgcaatc gcacgaaatc
     2221 gctgcaaagc ttgccaacga c
//
LOCUS       DATCBB010000694         8477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_314194, whole genome shotgun sequence.
ACCESSION   DATCBB010000694 DATCBB010000000
VERSION     DATCBB010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314194"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VII37_07710"
     CDS             complement(<1..799)
                     /locus_tag="VII37_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069658.1"
                     /translation="MNNLFRGFGAVFYKEVLHVRRDSATLFFSLFIPLIQMFVLGFGI
                     DTNVRQIHTVVFNGDGRRESRELIDRLKNSDTFNIIDYVATDRDLNDAIIAGRARVGI
                     KIPVDYSDRLIHNMSSQVLVLIDGSDSSVAGQAINVTTAIGLDESLRRVLADRASFAV
                     DMRPKLLFNPDSRSPNFLLPGLTAILLLNVTTFLTAFAIVREKERGTLEQLFVTPVRP
                     FGLLLGKLLPYFFIGFTELCLILTCMRFIFQVPIHGSVLLLAFLSLPY"
     gene            complement(792..1778)
                     /locus_tag="VII37_07715"
     CDS             complement(792..1778)
                     /locus_tag="VII37_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5069659.1"
                     /translation="MPPENQQRPEEASISAKNLVRRFGDFVAVDGVSFRVEKGEIFGF
                     LGPNGSGKTTVIKMLTGLLPLSEGSAWVEGLDVRKDAESVRERIGYMSQKFSLYDDLT
                     VAENLQFYGRIYSLPPARMKQRIDEIVQLNGLAPYMNRLAARLSGGWKQRLALGCAML
                     HEPKLLFLDEPTAGIAPVARRQLWDLLFELSGHGITFFVTTHYMDEAERCSHVAYIYY
                     GKLIADGTPVELKKIPDVQPAGTKRVEISTPEVTRALRVARKIPGIRSATIFGESIHA
                     LVEDRYDLPDLRAQLLKNDIAVTDIRPLTPSLEDVFVELTYKHQAQLETARE"
     gene            complement(1782..2885)
                     /locus_tag="VII37_07720"
     CDS             complement(1782..2885)
                     /locus_tag="VII37_07720"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5069660.1"
                     /translation="MSRKNIAILIVLIVLITAAVVSARWFQRRNDSALQGSGTVEARY
                     IRVGSKIGGRIDKVLVREGDSVKAGQVLVTFDDKELQAALGQSRAAWEKAHTGSRPED
                     IAQARAATAEAKADYEQRKNGYRTEDIAGAQSDLDRANAEEVRTHNDFERYDALAKKD
                     LVSRQQRDAAEAAWKVAAAQQKNAQQKLAELQRGYRPEEIASAEARYRAMLATQEKFE
                     RGSRSEDIDLAKAAYNYDEARAREAQVVSPADASVEVLDVRPGDLIAPNTPVATLLER
                     DQVYIRIYIPETEIGRVRLGQQAEVRVDSFPNTIFNGVVEQINQQAEFLPRNVQTRSE
                     RVHEVIGVKVRVNDTTGGVRSGMAADVKLKAAN"
     gene            complement(2882..3535)
                     /locus_tag="VII37_07725"
     CDS             complement(2882..3535)
                     /locus_tag="VII37_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5069661.1"
                     /translation="MALSTSTAPPDRRQRRSSQTRERLFRAALDLFARRGFADTTVED
                     ITNAADVGKGTFFNYFPSKDHMLLAFGEMQLGKLQSIIENLRRTREPVPQFFRTLAVR
                     MTEEPARNPSIVRALLLANLSSTPVREGMRKIQAQMHTLLSEFVQIGQQRGEVRDDLP
                     PGDIAQVFRQTVFGTLLVWSLYGDDSLATRIRTALDILWQGLSPRRFGASNAAGDAT"
     gene            complement(3709..4422)
                     /locus_tag="VII37_07730"
     CDS             complement(3709..4422)
                     /locus_tag="VII37_07730"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY5069662.1"
                     /translation="MKRMLSFLALAALALPSSGAGSKETERLQNCGTVLREIMDIPED
                     IPQDLIDKAECVIVFPSVLKAAFVVGGSYGRGAMTCRTGEHYSGPWSAPSMMALEAGS
                     VGFQIGGQATDFVLLVMNPRGAHAMLKSKVKLGADASAAAGPKGRTAEASTDLTLRAE
                     VLSYSRSRGLFAGVSLAGASLRPDGDANERVYGKGVDAHEIIFGHGVKTPKAAQRMIA
                     YLNRRTPKNTSDPRSLRED"
     gene            4642..6225
                     /gene="dacB"
                     /locus_tag="VII37_07735"
     CDS             4642..6225
                     /gene="dacB"
                     /locus_tag="VII37_07735"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY5069663.1"
                     /translation="MAVAICGLVFCGLFLNASALPALPAQKKTNRHSGTSTSAAAATS
                     GFAKRVDTLLGAEPASKGEWGLLVADAETGQTLYEQNAAKYFVPASNMKLFTTTLALD
                     KLGADFRFHTTLESAGVVSPEGELSADLVLVGRGDPNLSNRKFPFNLKEEFDGPPERA
                     LAELADALVAKGIKKISGDVIGDDSYFPRERYPNGWEIDDMVWEYGAAISAIVVNDNT
                     VTVTLTPGQVGDAVQAVVSPHTPDFLMDNEVTTSAANAKPDLTLTREPGAHLVVVRGT
                     LPEKSAPRKLVLAIEEPALHAATLLKVLLEQRGVPVDGGVLARHETITAEGITPTVLA
                     EHVSIPLGDSVKLVNKISQNLHTEVLLRTAMRQAGTWNFFDDVAKFAAGFYARVGIAA
                     QDIIQTDGSGLSRHDMVTPRAMLSLLRYAQTRPWFESFFASLPVAGVDGTLEERMKNT
                     PAAGRVHAKTGSVEHVRTLSGFADTAGGRRLAFSFMTNNEYGKNHETTDAMNGLCLAM
                     IEEFNAVPAKRSREHKQPQ"
     gene            6238..6798
                     /locus_tag="VII37_07740"
     CDS             6238..6798
                     /locus_tag="VII37_07740"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY5069664.1"
                     /translation="MKKEMIVSAFGSSRPRENDSEYTQARELGRALAGKGFAVCTGAY
                     AGTMEAVSRGAKEAGGKTYGVTAEFFPAAKANKWVDVEVRVKTWQDRLFEIIRLGDGF
                     VACKGGTGTLAELAVAWEMMNKSVMPTKPFVALGDFWAPILNCVRGVEVGHENPWAEA
                     QAQISTTAATPEKAAEFLWNSLEKEM"
     gene            6805..>8477
                     /locus_tag="VII37_07745"
     CDS             6805..>8477
                     /locus_tag="VII37_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069665.1"
                     /translation="MSRTALDVLEFDKLLELLRQRTTCAPGRRFVDGLQPGMDRAAIT
                     LGFALIREAREWLRAARELGFGGLADPQNWLERIEGPGMVLEPREFLDAATLLETAGW
                     LRQQFREEETKFPLLAARAAALSEFKDLLAVIRRSVLPNGEISDDASPALRRIRASMN
                     QTRDAIQKTLKHILRTKNAEAGEDYVTLRNDRFVIPLRAENRRSVPGVVHGASATGQT
                     IFMEPLETVETNNQLVQLREDEAAEIVRILRELTERLQRVREPLVLASGTIAELDSVF
                     ARARFTQEFDAAIPEFSEGSELRLEAARHPVLENKLRKEGRAVVPMSLELGGEERVLV
                     ISGPNTGGKTVALKTTGIAALSTQSGIPVAAQRAVLPFFDRVLVDIGDEQSIAADLST
                     FSAHMLNLKAMLEAATPESLVLVDEMGTGTAPEEGAALAVALLDEFRAKNCIVLATTH
                     HDRLKTYASTTPGVVNAAVEFDDVNLRPTYRLMVGVPGGSSGIAIARRLGIAEPIIAR
                     ARSLMAPEALEAADLIAYLHRSRDELDRMQQQMTAERHALDEERKKLRD"
BASE COUNT         1921 a         2227 c         2583 g         1746 t
ORIGIN      
        1 ggtaaggcaa ggagagaaaa gcgagcagca gaacgcttcc gtggataggc acctgaaaaa
       61 taaaacgcat gcaagtaaga atcaggcaca gctcagtgaa cccgatgaag aaatacggaa
      121 gcaattttcc cagtagcaat ccgaacggac ggaccggcgt tacgaacaat tgctcaagcg
      181 tgccgcgttc tttttcgcga acgatagcga aggcggtgag aaacgtggtg acgttcagaa
      241 gcaagatggc ggtaagcccg ggcagcagga aatttggaga gcgcgagtcc ggattgaata
      301 gcaatttcgg ccgcatgtcc acggcgaagg aagcgcggtc agcgaggacg cggcgaagcg
      361 attcgtccag cccgatggcg gtggttacgt taatggcctg acctgcgacc gaggaatcag
      421 agccatcgat gagcaccaaa acttgcgaac tcatgttgtg gatgaggcga tcagaatagt
      481 ccacgggaat ctttatgccc acgcgagcgc gtccggcgat gatggcgtca ttaagatcgc
      541 ggtcggtcgc aacgtaatcg atgatgttga aagtatcgga gtttttgagg cggtcgatca
      601 gttcgcgact ttcgcgccgg ccgtcgccat taaaaaccac ggtgtggatc tggcggacgt
      661 tggtgtcaat tccaaaacca agcacaaaca tttgaatcag cggtatgaac agtgagaaaa
      721 acagcgtggc cgaatcacgc cgcacgtgca gcacttcttt gtaaaaaact gcgccgaagc
      781 cgcgaaagag attattcacg ggcggtctcc aattgcgcct ggtgtttgta ggtgagctcg
      841 acaaacacgt cttcgagact tggtgttagc gggcggatgt cagttactgc aatatcgttt
      901 ttgagcagct gcgcacgaag atccggaagg tcgtaacggt cttccaccag cgcgtgaatg
      961 gattcgccaa aaattgtggc gctgcgaatt cccggaattt tgcgcgccac ccgcagcgcg
     1021 cgagtcacct caggagtgct gatctcgacg cgcttggtgc cggcgggctg cacgtctgga
     1081 attttcttca gctcaacggg agtgccgtcg gcaatgagtt tgccgtaata gatgtaggcc
     1141 acgtgactgc agcgctcggc ttcgtccatg taatgcgtag tgacaaaaaa ggtgatgccg
     1201 tggccggaga gctcgaacag caaatcccat aattggcgcc gcgcgacggg ggcgatcccc
     1261 gccgtgggct catcgagaaa caaaagttta ggctcgtgaa gcatggcgca accgagcgcc
     1321 aaacgctgtt tccatccacc agaaaggcgc gccgcgagcc gattcatgta tggagccagc
     1381 ccattgagct gaacgatttc atcgatacgc tgcttcatgc gtgccggcgg caagctatag
     1441 atgcgtccgt agaattgcaa gttttcggca accgtgaggt catcgtacag actgaatttt
     1501 tgagacatgt aaccgatgcg ctcgcgaacc gattccgcgt ccttgcgcac gtcaaggcct
     1561 tccacccagg cggacccttc gctcagaggc aacaggccag taagcatttt aatgacggta
     1621 gttttgccgc tgccgttggg accgaggaag ccaaatattt cccctttttc gacgcggaag
     1681 ctgacgccgt caaccgccac aaagtcgcca aaacggcgaa ccagattttt cgcggagatg
     1741 gaggcttctt cggggcgctg ttggttttcc ggaggcataa attaattcgc agctttcaac
     1801 ttcacgtcag ccgccatccc actgcgaaca ccaccggtgg tgtcgttgac gcgaactttt
     1861 acgccgatta cttcgtgcac ccgttcgcta cgagtctgca cgttgcgagg aagaaattcg
     1921 gcttgctgat taatctgctc cacgacgcca ttgaaaatgg tgttgggaaa agagtcgaca
     1981 cgaacttccg cttgctggcc caagcgcaca cggccaattt ccgtctcagg gatataaatg
     2041 cggatgtaca cctggtctcg ctccagcaat gtggcaaccg gcgtattggg tgcaatcaaa
     2101 tctcccggac gcacgtccaa gacctccacg gatgcatccg ccggcgaaac cacttgcgct
     2161 tcacgcgcac gcgcttcgtc atagttataa gcagctttgg caagatcaat atcttcactg
     2221 cggctgccac gctcgaactt ttcctgtgtt gccaacattg cgcgatagcg cgcttccgct
     2281 gaggcgattt cttccggacg gtagccgcgt tgaagttcgg ccaatttttg ctgggcattt
     2341 ttctgctggg ccgccgccac cttccaggcg gcttccgcgg catcccgttg ttggcgcgaa
     2401 accagatctt ttttcgctag agcgtcatag cgttcgaaat cattgtgcgt gcggacttct
     2461 tccgcattgg cgcgatcgag gtccgattga gccccagcaa tgtcttcagt gcggtagccg
     2521 ttcttgcgct gctcgtagtc ggctttggcc tcggcggtcg cggcgcgcgc ctgcgcgata
     2581 tcttccgggc gcgaaccggt gtgagctttt tcccacgcgg cgcgcgactg cccgagcgcg
     2641 gcctgcagtt ccttgtcgtc aaaggtaacc aaaacctgcc cggccttcac ggaatcgccc
     2701 tcgcgcacca ggactttgtc gatacgtccg cctattttgg agcccacccg gatataacgc
     2761 gcttccaccg tgccagatcc ctgcaaggcg gaatcgttgc gtcgctgaaa ccatcgtgcg
     2821 gatacgactg cagcagtaat gaggacgatc aacacaatta aaatcgcaat attcttacga
     2881 ctcatgttgc gtctcctgcc gcgttagagg cgccaaaccg ccgcggagac aaaccctgcc
     2941 aaagtatatc gagtgcggtg cgtatgcggg tggccagcga atcgtctcca tataatgacc
     3001 aaaccaggag ggtgccaaaa actgtttggc gaaagacttg agcaatgtcg cctgggggca
     3061 aatcgtcgcg cacctcgccg cgctgctgac cgatttgtac aaactcactc agcaatgtat
     3121 gcatctgagc ctggattttg cgcataccct cgcgcaccgg ggtgctggac aaattggcga
     3181 ggagtagcgc gcgcacgatg gagggattgc gagcaggctc ctccgtcata cgcacagcaa
     3241 gtgtgcgaaa gaattgcggg acgggctcgc gagtgcggcg aagattttca ataatggatt
     3301 gcagcttgcc aagctgcatt tcaccgaaag ccagtagcat gtgatctttg ctgggaaagt
     3361 aattaaaaaa cgtgcctttg ccgacgtcgg cggcgttagt aatgtcttcc acggtggtgt
     3421 ccgcaaaacc acgccgagca aaaagatcga gagcagcgcg aaataggcgc tctcgggttt
     3481 gcgaagagcg ccgctggcga cgatctggtg gggcggttga ggttgaaagg gccatatgac
     3541 cattacccat atatgactat aagtcatgtt gccgcgtggg acaagctttc agattgctgg
     3601 aagatccaaa gcgacacgaa aaatgaaacg cccaagcacg agagtcttgg gcgttcattg
     3661 ccgttcggag gcctgggaag ggcttcaacg cccctctacg caatattgct agtcttcgcg
     3721 cagagaccgg ggatccgacg tgttcttcgg ggtgcggcgg ttgagatagg caatcatgcg
     3781 ctgggccgct ttcggcgttt ttacgccgtg gccgaagatg atctcgtgag cgtcgacgcc
     3841 tttgccatac acccgctcgt tggcgtcacc atccggccgt aggcttgctc ctgccaacga
     3901 aactccggca aagagcccgc gcgaacgtga gtagcttaga acctcggcac gcagtgttag
     3961 gtcagtggaa gcttcggcag tgcgtccctt ggggccagca gcggctgaag cgtccgcacc
     4021 tagttttacc ttgctcttca gcatggcgtg agccccgcga ggattcatca ccagcaggac
     4081 gaagtccgta gcttgtccgc cgatctgaaa cccaacgctg ccagcttcca aagccatcat
     4141 ggagggcgcg ctccaaggac cgctgtagtg ctcaccggtt cggcaagtca tggcgccgcg
     4201 gccgtagctg ccaccgacaa caaacgcagc tttgaggacg gaggggaaaa caattacgca
     4261 ttcggctttg tcgatcaggt cttgcggaat gtcttctgga atatccatga tttcccgcaa
     4321 caccgtaccg caattctgaa gtctttccgt ttcttttgaa cctgcgccgg acgatggcag
     4381 cgccagcgcg gccagagcca ggaaggaaag cattcgcttc atttttgatt cacctcggtt
     4441 tcgatttttg acgcaatagt tggaaaaacg gttaacgtgg acgccaccgg cggacattgc
     4501 gttctcagac ccaagcgcgc aacgccaatg ttaagcgagc gctctaaggg gagtgatccg
     4561 cggcgcttcg actatacttt cgttgaccgc aatttaccag cagggagcgc aatgggcatc
     4621 aagcgagcga cttggaaacg cgtggcagtg gcgatatgcg gacttgtgtt ttgcggtcta
     4681 tttttgaatg catcagcatt gccagcatta cctgcgcaaa agaaaacgaa ccgccacagt
     4741 gggacttcca cgagcgcagc cgcggccacg tctggcttcg ccaagagggt agatacgctg
     4801 cttggcgcag agccggccag taaaggggag tggggcttgt tagttgccga tgcggaaacg
     4861 ggccaaacgc tgtacgagca gaatgcagcg aagtatttcg tgcccgcatc gaatatgaag
     4921 ctgtttacca ccacactcgc gctcgacaaa ttgggagcgg attttcgttt tcataccacc
     4981 ctggaaagcg cgggagtggt aagcccagaa ggtgagttgt ccgcggacct ggtgctggta
     5041 ggccggggcg accccaatct ttcgaatcgc aaattcccgt ttaatttgaa agaagaattc
     5101 gatggaccgc cggaaagggc tctggccgag cttgctgacg ctttggtggc caagggcata
     5161 aagaaaatct ccggcgacgt aattggagat gacagctact ttccgaggga gcgctatccc
     5221 aatggttggg aaattgacga catggtctgg gaatacggcg cggcgatttc cgcgatagtt
     5281 gtaaacgaca acaccgtgac agtgacgctt acgccgggcc aggtaggtga cgcagtgcag
     5341 gccgtcgtat cgcctcacac tccggatttt ttgatggata acgaagtaac gaccagcgcg
     5401 gcaaatgcaa aacctgattt gacgctgacg cgtgagcctg gcgcgcacct ggtggtggtg
     5461 cgcggaacgt tgccggaaaa aagcgctccg cgaaaattgg tgctagcgat tgaggaaccg
     5521 gcgttgcatg ccgccacatt attaaaggtg ctcctggaac aacgcggagt accagttgac
     5581 gggggagtac tcgcgcggca cgaaaccatt acggccgagg ggatcacgcc gactgttttg
     5641 gctgagcatg tgtcgatacc tttgggcgac tccgtcaagc tggtgaacaa gatcagtcag
     5701 aatttgcaca ccgaagtgtt gctgcgcacc gccatgcgcc aagcaggcac ttggaatttc
     5761 ttcgatgacg tggcgaaatt tgcggcgggc ttttatgccc gggtgggaat cgctgcgcaa
     5821 gacataattc agacggatgg gtctggatta tcgcggcacg acatggtcac gccgcgagcg
     5881 atgctcagct tgttgcgtta cgcgcaaacg cggccctggt ttgaaagctt ttttgcgtcc
     5941 ttgccggtgg ccggcgttga cggaaccctt gaagagcgca tgaaaaacac gcctgcagcc
     6001 ggaagagtgc acgccaaaac aggatcggta gaacatgtca ggacgctgtc gggattcgcc
     6061 gacacggcgg gcgggcgcag attggcgttc tcttttatga ccaacaatga atacggaaag
     6121 aatcacgaga ccacggacgc gatgaacggg ctgtgcctgg cgatgattga agaattcaat
     6181 gctgtgcccg ctaaacgctc ccgcgaacac aagcaaccac agtaagcagc agggaaaatg
     6241 aaaaaggaaa tgattgtcag cgcgtttggt agttcgcggc cgcgcgaaaa tgattcggag
     6301 tatacccagg cgcgcgagct ggggcgcgcg ctagcgggaa agggctttgc agtatgcaca
     6361 ggcgcgtacg ccggaacgat ggaagcagtg tctcgcggcg cgaaagaagc tggcggcaag
     6421 acttacgggg tgaccgcgga attttttcct gcggccaaag ccaataaatg ggtggacgtg
     6481 gaagtacgcg tgaaaacctg gcaggacagg ttatttgaaa ttattcgctt aggggatggg
     6541 ttcgtcgctt gcaaaggcgg cacgggaact ttggcggagt tggcagtggc ttgggaaatg
     6601 atgaacaagt cagtgatgcc caccaaaccg ttcgtggcgc tcggagactt ttgggctccc
     6661 attttgaatt gtgtccgcgg ggtggaagtg gggcacgaaa atccctgggc cgaagcacag
     6721 gcgcagattt cgacaacggc ggccactcca gaaaaggccg cggagttttt gtggaatagc
     6781 ttagaaaagg aaatgtaacc gagcatgtcg cgcacggcgc tggatgtatt ggaattcgac
     6841 aagctgctgg aattgctgcg gcagagaacg acttgcgcgc cggggcgaag atttgtggat
     6901 ggcttgcagc ccgggatgga tcgcgcggcc attacgctgg ggtttgcgtt gattcgcgaa
     6961 gcgcgtgaat ggctgcgtgc cgcccgagaa ttaggatttg gagggctggc cgatccacaa
     7021 aactggctcg agcgtattga agggccggga atggtcctcg aaccccgcga atttttggat
     7081 gctgccactt tgctggagac tgccggatgg ttgcgccagc agtttcgcga agaagagacc
     7141 aaattcccgc tgctggccgc gcgtgctgcg gcgctgagcg agttcaaaga tttgctggcg
     7201 gtcattcgcc gaagcgtgct gcccaatggc gagatcagcg acgacgcgtc accggccctt
     7261 cgaagaatac gcgccagcat gaaccagacg cgtgatgcca ttcagaaaac gctgaaacat
     7321 attttgcgca ccaagaatgc cgaggccggc gaagactacg ttactttgcg caatgaccgc
     7381 ttcgtgattc ccttgcgcgc ggagaatcgc cgcagcgtgc ctggcgtggt tcacggggct
     7441 agcgccactg gacagacgat ttttatggag ccgctggaga cggtcgagac caataaccag
     7501 ctggtgcaac tgcgcgagga tgaggccgcc gaaattgtac ggatattgcg tgaactcacg
     7561 gaaagattgc agcgcgtgcg agagccgtta gtgttggcgt cgggaaccat cgcggagttg
     7621 gacagcgtct ttgcgcgggc aaggttcaca caggaatttg atgctgctat accggaattt
     7681 tcagaaggca gcgagctacg gctggaggcc gcgcggcatc cggtgctgga aaacaaactg
     7741 cgcaaggaag gccgcgccgt ggtgccgatg agcctggagc tcggcggcga agagcgcgtg
     7801 ctggtgatca gcgggccgaa caccggcggg aaaacagtag cgctgaagac caccgggatt
     7861 gcggcgctgt ctacacaatc ggggattccg gtggcggcgc aacgcgcagt gcttccgttt
     7921 tttgaccgcg tgctcgtgga cattggagac gaacagtcca ttgccgccga tctttccact
     7981 ttttccgcgc acatgttgaa tctgaaagcg atgctggaag cggccactcc cgagtccctg
     8041 gtgttagtcg atgaaatggg cacggggact gcgccggagg aaggggcagc gctggcggtg
     8101 gcgctactgg atgaatttcg cgcgaagaat tgcattgtgt tggccaccac gcaccatgat
     8161 cgtctgaaaa cgtacgcctc gacaacgcca ggagtggtga acgccgcagt ggaatttgac
     8221 gatgtgaatc ttcgaccgac gtaccggctg atggttggtg tgccgggcgg gtccagcgga
     8281 atcgcgatcg cgcgacgatt agggattgcc gagccgatca tcgctcgtgc gcgttccctc
     8341 atggccccgg aagcgctcga agccgcggat ttgattgcct acctgcatcg cagccgggat
     8401 gagctggacc gcatgcagca gcagatgacc gcggagcggc atgcgctcga tgaagaacgc
     8461 aagaaattac gcgatga
//
LOCUS       DATCBB010000695         1674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_443635, whole genome shotgun sequence.
ACCESSION   DATCBB010000695 DATCBB010000000
VERSION     DATCBB010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443635"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VII37_07750"
     CDS             complement(<1..229)
                     /locus_tag="VII37_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5069666.1"
                     /translation="MSKGSILVVDDESEIREGLELLLTGEGYGVSSAGTGESGLAKLG
                     EHPFDLLLLDVSLPDRNGLDLLKEIHKRDPHL"
     gene            complement(222..>1674)
                     /locus_tag="VII37_07755"
     CDS             complement(222..>1674)
                     /locus_tag="VII37_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069667.1"
                     /translation="RLAIFQENSTHPGHMRLARSMGVRLTEPLDLSFLEPARPEFARG
                     ALFFESPRAARDVSESVRRTLEQLDLNYFVPCRIREHTVAVLGLGKTVDGDFLSSDDV
                     ELVETIAGYVAIALDNSQLYTSLEQKATEIARLKDFSENIVESLNVGVLAVDLEGLVE
                     SWNTRMEQLFGVVREQAMGRHLNSLLPEELSKEINARSDDELITGIYKQRLLHLGKTL
                     TLNVSITPLVSKSGERIGRLLLFDDVTQRERLEEQMTQTEKLTSLGLLAAGVAHEVNT
                     PLAVISNYIQMLGKQMPDGDPRQAIIEKIVKQTFRASEIVNNLLNFSRTSATEVVDID
                     VNRVVEETLSLVAHPLKTSQIQVVRQLGETLPAVRGSANKLQQVFLNLFLNARDAMPG
                     GGMLEVRTTAHNGSVEVEVVDTGAGIAREHIHRIFDPFFTTKASGRGTGLGLSVSYGI
                     IKEHSGKIDVRSTVGKGTSFHVELPAVRKAIHV"
BASE COUNT          359 a          541 c          393 g          381 t
ORIGIN      
        1 aaagatgcgg gtcccgtttg tgaatttcct tcagcaggtc cagaccattg cggtccggca
       61 ggctcacatc cagcaacaac aaatcgaaag gatgttcccc caatttcgca agcccggatt
      121 cgcccgtccc cgcgctcgaa actccgtaac cctcaccggt taggagaagc tcaagtcctt
      181 cgcgtatttc tgattcatca tccaccacca gaatcgagcc cttagacatg aatcgctttc
      241 ctgaccgccg gcagctctac gtggaacgaa gtccccttac ccacagtcga gcgcacatca
      301 atttttccgg agtgttcttt aatgattccg taactcaccg acagccctag cccggtcccg
      361 cggccgctcg ccttggtggt gaagaatgga tcgaatatcc ggtgaatgtg ttcgcgcgca
      421 atccccgccc cggtatcaac gacttccact tccacacttc cattgtgggc cgtcgttcgc
      481 acttccagca ttcccccgcc gggcatggca tcgcgcgcat tcaaaaatag attcaaaaac
      541 acctgctgca gcttgttcgc tgatccacgc actgctggaa gcgtttcgcc cagttgccgt
      601 accacctgaa tctgcgaagt cttgagcggg tgcgccacca gcgaaagtgt ttcttccacc
      661 acgcgattca catcgatatc caccacttcc gtcgcgctgg tgcgggaaaa attcagcaaa
      721 ttattcacaa tttcgctggc gcgaaaagtc tgctttacga ttttctcgat gatcgcttgc
      781 cgcggatcac cgtcgggcat ttgttttccc agcatctgta tgtaattgga gatcaccgct
      841 agcggcgtat tgacttcatg cgccacgccg gctgccagca gccccaatga cgtgagcttc
      901 tccgtctggg tcatctgttc ttccaaccgc tcgcgttgcg tcacatcgtc aaatagcagt
      961 aaccgcccga tgcgctcacc cgatttgctt accagcggcg tgatggaaac atttaatgtc
     1021 agtgtcttcc ccagatgaag cagccgctgc ttgtaaatcc cggtgatcaa ttcatcgtcg
     1081 ctgcgtgcgt taatctcttt cgacaattct tccggcagca gggaattcaa atgacgcccc
     1141 atcgcttgct ctcgcaccac gccaaacaac tgctccattc gcgtgttcca ggattccacc
     1201 agcccttcca ggtccaccgc cagcactccg acgtttagcg actccacgat gttttcgctg
     1261 aagtccttca accgcgcgat ctctgtggcc ttttgttcta gcgaggtata tagctgcgaa
     1321 ttatccaacg cgatggccac gtaaccggcg atggtctcca ccaactccac gtcgtcgctg
     1381 gaaagaaaat ccccgtccac ggtcttgccc aggcccaaca ccgccacggt atgctcccga
     1441 atccggcaag gcacaaaata atttaaatcc aactgttcca gcgtgcggcg caccgattcg
     1501 ctcacatctc tcgccgcgcg gggcgactca aaaaagagcg ccccacgggc gaactcgggc
     1561 cgggccggct cgaggaaact aagatcgagt ggttcagtta ggcgcacgcc catcgaccgc
     1621 gccagccgca tatgacccgg atgcgtcgag ttttcctgga aaattgccag ccgg
//
LOCUS       DATCBB010000696         1134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_478520, whole genome shotgun sequence.
ACCESSION   DATCBB010000696 DATCBB010000000
VERSION     DATCBB010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478520"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VII37_07760"
     CDS             <1..672
                     /locus_tag="VII37_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5069668.1"
                     /translation="KNAPAVKFRAVESYGAQVTLCEPTVTARNETAARVRAETGAYLI
                     HPYDDDRIISGQATAAKELLEEIPDLDAVFAPVSGGGLLSGTCLGAKGLRADIRIFGC
                     EPERADDAYRSLASGTLQSQDSSDTIADGLRASLAPRTFAILRKLVDGILLASEEEII
                     SATRLIWERMKIIIEPSSAVAIAPLLRPGAVTALNLPPRQDGRPAKLGVIFSGGNVDL
                     SSLPF"
     gene            complement(893..>1134)
                     /locus_tag="VII37_07765"
     CDS             complement(893..>1134)
                     /locus_tag="VII37_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069669.1"
                     /translation="SYEELKAKVAELEKKGSGRRTGALEFRVGEKGGVSVYGLGRFPV
                     TLYYEQWVRLLDATQDLRAFLEENKAGGKLKLRET"
BASE COUNT          235 a          377 c          261 g          261 t
ORIGIN      
        1 aaaaacgcgc ccgccgtaaa attccgcgcc gtcgaaagct acggagcaca agtcactttg
       61 tgcgaaccga ctgtcaccgc gcgaaatgaa accgccgctc gcgttcgcgc cgagacgggc
      121 gcctatctca tacatcccta tgacgacgac cgcatcatct ctggacaagc caccgcagcc
      181 aaagaacttc tcgaagaaat ccccgacctc gacgccgtct ttgctcctgt gagtggcggc
      241 ggcctgctca gtggcacgtg cctcggcgca aaaggcctgc gcgctgatat tcgcattttt
      301 ggttgcgagc ccgaacgcgc cgatgacgcc tatcgctccc tcgccagcgg cacattacag
      361 tctcaggact ccagcgacac catcgccgac ggtttgcgcg cctctctcgc tccccgcacc
      421 ttcgccattc ttcgaaaact tgtagatgga attttgctgg ccagtgaaga agaaattatt
      481 tccgccacac gcctcatttg ggagcgcatg aaaatcatca ttgagccatc atccgccgtg
      541 gccatcgccc cgttgcttcg gcccggcgca gtgaccgcct tgaatttacc gccgcgccag
      601 gatggccgcc ccgctaaact cggcgtaatc ttttccggcg gtaatgtcga cttatcgtcc
      661 ctaccctttt aaatttttta gtttcttctt tcgacaggaa gaacacttcg cgccactcat
      721 aacccggcag ggtgccccag gttcgcagtt gagttatgaa cctgggtctg cggttgactc
      781 ttcccggaaa ccacgtgcgc cactcatcgc aattccaaac aacgcgggac gggtgcccca
      841 ggttcgcagt taagttatga acctgggtct tgcggttgac tcttctcgga atctacgttt
      901 cccgcagttt caatttaccg ccagctttat tttcttccag aaacgcccgc aaatcctgag
      961 tagcatccag caaccgcacc cactgctcgt aataaagtgt gaccggaaag cgtcccaacc
     1021 cgtacacact tacgccaccc ttttcgccta cacgaaattc cagcgccccg gtgcgccgcc
     1081 cggagccttt cttttccagc tccgctactt ttgccttcaa ttcttcatat gatg
//
LOCUS       DATCBB010000697         1105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_412351, whole genome shotgun sequence.
ACCESSION   DATCBB010000697 DATCBB010000000
VERSION     DATCBB010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412351"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..>1105
                     /locus_tag="VII37_07770"
     CDS             49..>1105
                     /locus_tag="VII37_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEY5069670.1"
                     /translation="MTGSEAVHQLCLEANANSNCIGLIAWMHTFSPARMWIAGLRNLA
                     KPLAHLHTQFNEELPWSTIDMDFMNLNQSAHGDREFGYIGSRMRLQRKVIVGYWKDKN
                     IQSELGTWTRAACAWHDAQHLKISRFGDNMRNVAVTEGDKVEAQIRLRYTVNGFGVGD
                     LAQSVSDVSDAEADKLTREYDDAYAVAETLRPAGRQRQSLREAARIELGVRRFLTENN
                     SYAFTDTFEDLRGLEQLPGIAVQRLMADGYGFGAEGDWKAAALVRAMKVMSHGLPGGT
                     SFMEDYTYHLKDGGQVLGAHMLEVCPSIAKGKPSAEIHPLSIGGKADPVRLVFTASSG
                     PAVNASIVDMGNRFRLIV"
BASE COUNT          251 a          278 c          330 g          246 t
ORIGIN      
        1 ttgtccggct gttctgcgat acccgttaaa gttataccca agccagtcat gactggcagc
       61 gaagctgtcc atcaactttg tttggaagct aacgcgaatt ccaactgcat tgggttaatt
      121 gcctggatgc atacattttc ccctgctcgc atgtggatcg cgggcctgcg gaacctggcc
      181 aaaccgttgg cgcacctgca tacgcagttc aacgaagaat tgccctggtc taccatcgac
      241 atggacttca tgaatttaaa tcaatccgcg catggcgacc gcgaattcgg atacatcggg
      301 tcgcgcatgc ggcttcagcg caaggttatt gtcggctact ggaaagacaa aaatattcag
      361 tccgagttag ggacatggac gcgggcagcc tgtgcatggc atgacgccca gcacttaaaa
      421 atttcacggt tcggcgataa catgcgcaac gtggccgtca cggaaggcga taaagtcgaa
      481 gcgcagattc gcctgcgata taccgtcaat ggtttcggcg tcggagatct ggcgcaaagt
      541 gtaagcgacg taagtgacgc ggaggctgac aagctcactc gggaatatga tgacgcgtat
      601 gcggttgctg aaacgttgcg tcccgcgggg cgtcagcgcc aatcattgcg cgaagctgct
      661 cgcatcgagc tgggagtgcg gcgatttttg actgaaaaca attcgtacgc gttcaccgat
      721 acgtttgagg atctgcgcgg cctggagcaa ttgcctggca ttgcggtgca gcgcttgatg
      781 gccgacggct acgggtttgg ggcggaaggc gattggaagg cagcagcgct ggtacgcgcg
      841 atgaaggtga tgagtcacgg actcccgggt ggcacgtcgt tcatggaaga ctacacgtac
      901 catctgaaag atggcggcca agtgttggga gcgcacatgc tggaggtatg tccatccata
      961 gcaaaaggca agccctcggc ggagatccac ccactttcta tcggaggaaa agctgatccg
     1021 gttcggttgg ttttcactgc ttccagcggg cctgcggtca acgcttctat cgtggatatg
     1081 ggaaaccgct ttcgacttat cgtaa
//
LOCUS       DATCBB010000698         1375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_153339, whole genome shotgun sequence.
ACCESSION   DATCBB010000698 DATCBB010000000
VERSION     DATCBB010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153339"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VII37_07775"
     CDS             complement(<1..630)
                     /locus_tag="VII37_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069671.1"
                     /translation="MGRAFRWAQPVVFYVGTLLIATGTLRAQDPLGIFAPAMPHGIPQ
                     DWSHRHIIYTRNGSVEDMMRLRDDPRFLHSVVLHYMREHRNQTGQSATAGLNEAGWNE
                     SNLGEGTTAADTQESRAIAPWHPIRPIFPARNKRPKVDWSVSLGPTAGLALGETPAVY
                     TYNYAKPSCSNLSATPPVIGDFVVYTINAAAAVGKPGQANLVGLTNLYTA"
     gene            complement(767..1366)
                     /gene="recR"
                     /locus_tag="VII37_07780"
     CDS             complement(767..1366)
                     /gene="recR"
                     /locus_tag="VII37_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY5069672.1"
                     /translation="MSRFAEPMTRLIDELKKLPGIGSKSAQRLAFHILRSSEDDAESL
                     ASAVRDVKAKLRLCSICNNITDVDPCVYCSSPTRNQKLVCVVEEPTNISSVEKTRHFN
                     GVYHVLHGSISPLHGVGPDQLRIANLITRVATGEIEEVILATNPTVEGEATATYLAQQ
                     LKRQGLRVTRIAMGIPVGSDIEYTDEVTMLKAMEGRREL"
BASE COUNT          266 a          359 c          433 g          317 t
ORIGIN      
        1 ggcggtgtag agattggtca atccgaccag attggcttgg cccggcttgc ctaccgccgc
       61 agccgcgtta atcgtgtaca cgacaaagtc gccgataacc gggggcgtgg cgctgagatt
      121 actgcaactg ggctttgcat agttataggt gtagactgcc ggggtttcgc ctaacgccag
      181 gccggcggtt ggccccaggg aaacggacca gtcaaccttg gggcgcttgt ttctagcagg
      241 gaaaatcgga cggattgggt gccacggagc gatcgcgcgt gattcttggg tatcggcggc
      301 ggtggttcct tcgcccaaat tactttcatt ccatccagct tcattcagcc ctgctgtcgc
      361 cgattgtccg gtttgattgc ggtgctccct catgtaatgc agaacaacgc tatgcaggaa
      421 gcgtggatcg tcgcggagcc tcatcatgtc ttccaccgag ccgttgcggg tatagatgat
      481 gtgccggtgg ctccaatcct gcgggatgcc atgcggcatg gcgggagcga aaattccgag
      541 cggatcctgc gcgcgcaacg tgccggtcgc tatcaacagc gtaccgacgt agaaaacgac
      601 tggttgcgcc cagcggaagg cacggcccat aggttgatcc cccgaaaaaa gcatttcggg
      661 taattatagg ggggggccag ggtgccaact agtgcctttc gattgacctt tcggatgctt
      721 tggttaaaga gtatggaact tcgtttgctg gcgcccgggg caaacgctac agttcgcggc
      781 ggccttccat ggccttcagc atggtgacct cgtcggtata ttcaatgtcg ctgccgactg
      841 gaattcccat ggcgatgcgg gtgacgcgga gaccctggcg tttgagttgc tgcgccaggt
      901 aggtggccgt ggcctcaccc tcaactgtgg gattggtggc caggatcact tcttcgatct
      961 ctccggtagc cacgcgcgtg atcaaatttg cgatgcggag ctggtccggg ccaactccat
     1021 gtaaggggga gatcgagccg tggagcacgt ggtagacgcc gttaaagtgc cgcgtttttt
     1081 ctaccgaaga gatgttggtt ggttcctcca ccacgcaaac aagtttttga tttcgcgtcg
     1141 ggctactgca ataaacgcag ggatcgacat cggtgatgtt gttgcaaatg gaacacagcc
     1201 gcagtttggc tttcacatcg cgcacagcgg acgcaagaga ctcggcatcg tcctcgctcg
     1261 atcgcaggat gtggaatgcc aggcgctgcg cgcttttgct gccgataccc ggcagttttt
     1321 tgagttcgtc gatgaggcgg gtcatgggtt cggcaaatcg ggacatagaa agttg
//
LOCUS       DATCBB010000699         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_281832, whole genome shotgun sequence.
ACCESSION   DATCBB010000699 DATCBB010000000
VERSION     DATCBB010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281832"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..855
                     /locus_tag="VII37_07785"
     CDS             127..855
                     /locus_tag="VII37_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY5069673.1"
                     /translation="MAQEKIVVEADARSKRGKNEARRLRLTGKVPAVLYGGKGEAITL
                     AVNAKQLGAIFRSQSGHNTLFQVDLSGKHEPAILKDWLVDPVTGNLLHVDLLRVAMDV
                     RMRVKVPVHTFGEPAGVKQQGGVFEMVTREVEIECLPAEIPTEFKADISGLMLGQQLR
                     AADLAIDKNMKLVTDPERVLAHVVALKVEEEKPADAVAADAAAPAEPEVIKKGKKEVE
                     GEEGAEEAGKPKAAEKSDKGEKKK"
     gene            952..1545
                     /gene="pth"
                     /locus_tag="VII37_07790"
     CDS             952..1545
                     /gene="pth"
                     /locus_tag="VII37_07790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY5069674.1"
                     /translation="MRLIVGLGNPDPEYQWTPHNLGFMAVDELANRNGIRVSRPEGKA
                     LVGRGKLAGEDVLLAKPQTYMNLSGISVRELLEKYELGPSDLLVMWDEAQLPMGTIRI
                     SGEGSAGSHNGAKSVIGSLGTPVFTRLRLGCAPEHEVSSRKEYVLRPMRKSELEVAAE
                     MIAEAADAVEMIVAQGIDAAMNKYNRRKEPPAEETQA"
     gene            1593..2027
                     /gene="rpsF"
                     /locus_tag="VII37_07795"
     CDS             1593..2027
                     /gene="rpsF"
                     /locus_tag="VII37_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY5069675.1"
                     /translation="MEERLYDLIFIARPATADEDVKKVLSVIEHACTEKGGKVEKAET
                     WGTRKLAYKVAKHREGMYYYQQIRTSHPDLIAELERRLRVLDTVIKYQTVRLDEDLKR
                     QKKLMDRRDKRTARRPRRATTPPSAAAPSAPPAAPEHSAPAS"
     gene            2130..2681
                     /gene="rpsR"
                     /locus_tag="VII37_07800"
     CDS             2130..2681
                     /gene="rpsR"
                     /locus_tag="VII37_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY5069676.1"
                     /translation="MAEEQKQATAPSAPPRRDGAGGHGGGHSGGGHSGGGHSGGSHGG
                     PRREGGGPGGAGPGGPRRGKRQYFRKKKVCKFCVEKMDFIDYKRADILSQFVQERGKI
                     LPRRMTGVCSRHQRWLGVAIKRARNIALLPFAGSAAGTQQPRAVVAPAPGAPAGTPSS
                     SHAATPPAAATPAAAAPTEVSNA"
     gene            2733..>2942
                     /gene="rplI"
                     /locus_tag="VII37_07805"
     CDS             2733..>2942
                     /gene="rplI"
                     /locus_tag="VII37_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY5069677.1"
                     /translation="MQIILQEDIEKLGHRGDVVTVKPGYARNFLLPRKLAVEASAGNL
                     KAIEKIRGSLAKKTATEMEAAQKQAA"
BASE COUNT          706 a          667 c         1036 g          533 t
ORIGIN      
        1 ggatttattg gctcgcggga ttcgctcgat tcacgaagaa acgtccatca gcgaattgtt
       61 tatttgattt tcgaagaacc gatttataca cgggattgcg tagcaacatg agggaaggaa
      121 attaagatgg ctcaagaaaa aattgttgta gaagcggatg cccgcagcaa gcgcggcaaa
      181 aatgaagcgc ggcggttgcg gctgaccggc aaagtgcctg cggtattgta tggcggcaag
      241 ggcgaagcaa tcacgctggc ggtaaatgcg aagcaactgg gcgcgatctt ccgttcgcag
      301 agcggccaca acaccttgtt tcaggtggac ttgagcggca agcatgaacc ggcgattttg
      361 aaggattggc tggtggaccc ggtcacggga aatcttttgc atgtggattt gctgcgcgtg
      421 gccatggatg tgcgcatgcg cgtgaaagtt cctgtacata cctttgggga acccgccggc
      481 gtgaaacaac aaggcggcgt gtttgaaatg gtgacgcgcg aagtggagat tgaatgtttg
      541 ccggcggaaa ttccgacgga attcaaggcg gacataagcg gcttgatgct ggggcagcag
      601 ttgcgcgctg cggatttggc gattgataaa aatatgaagc tagtcaccga tccggagcgg
      661 gtactggcgc acgtggtggc gctgaaggtc gaagaagaga agcccgcgga tgcggtggct
      721 gcggatgctg cggcgcctgc ggagccggaa gtcatcaaga agggcaagaa ggaagtggaa
      781 ggcgaagagg gcgccgaaga ggcgggcaag ccgaaggccg cggagaagag cgacaagggc
      841 gagaaaaaga agtagcacgc ggcggtcggc gctacgggtt tctaggtcaa atgaactacg
      901 gatcgattca cgaaatgctt cacggaatgc ttttcgggga atgcggagtg tatgcggctg
      961 attgtaggac ttggcaatcc cgatccggag tatcagtgga cgccgcacaa cctgggattc
     1021 atggcggtag atgagctggc gaatcgaaac gggattcgcg tttcgcgacc tgagggcaag
     1081 gcgctggtcg ggcggggaaa gttagcagga gaagatgtgc tgctggcaaa gccgcagacg
     1141 tacatgaatc tgagcggcat ttcggtgcgt gagctgctgg aaaagtacga gctaggtccg
     1201 agcgatttgc tggtgatgtg ggacgaagcg cagttgccga tgggcacgat tcgcatcagc
     1261 ggcgaaggta gcgcgggcag ccacaacggt gcgaagtcgg tgatcgggtc gctggggacg
     1321 ccagtgttta cgcgattgag acttggttgc gcgccggagc atgaggtcag cagccgcaag
     1381 gaatatgtgt tgcggccgat gcgcaagagt gagttggaag tcgcggcgga gatgattgcg
     1441 gaagcggccg acgcggtgga aatgatagtg gcgcagggaa tcgacgcggc catgaacaag
     1501 tacaaccggc gaaaagaacc gccggcggaa gaaacgcaag cgtaaacggg tttgccgggc
     1561 ggctgcccgg agagaagaga aacgagggca acatggaaga gcggctatac gatttgattt
     1621 ttattgcgcg gccggcgact gcggacgagg acgtgaagaa agtgctttcg gtcatcgagc
     1681 atgcctgcac ggaaaagggc ggcaaggtgg agaaggcgga gacctggggc acgcgcaagc
     1741 tggcttacaa agtggccaag catcgcgaag gaatgtatta ctaccagcag attcgcacat
     1801 cgcatccaga tttgattgcg gagttggaac ggcgcttgcg agtgctggat accgtgatca
     1861 agtatcagac cgtgcggctg gatgaagatt tgaagcggca aaagaagctt atggatcgcc
     1921 gggacaaacg caccgcgcgt cgtccacggc gtgctaccac tcctcctagt gcggcagctc
     1981 catcggcgcc gccggcggct ccggagcata gcgctcccgc gagttagttt taaattgatc
     2041 gggctgcgtt tttttaggaa ttgagtcacg gctgaaaaca aaatttagtt gagattgttt
     2101 tttgcggttc aagtgaaagg gtggcgggta tggcagagga acagaaacag gcaacagcgc
     2161 cgagcgcacc tccgcggcgt gatggtgcag gcggtcacgg cggtggccat agtggcggcg
     2221 gacacagtgg cggaggccac agcggtggca gtcatggtgg gccgcggcgt gaaggcggcg
     2281 ggccgggcgg ggctggtcca ggcggaccac gtcgtgggaa gcgccaatat tttcgcaaga
     2341 aaaaagtctg caagttttgc gtggagaaga tggacttcat cgactacaaa cgcgcggaca
     2401 ttctgtcgca gtttgtgcag gaacgcggca agattctacc ccggcgcatg actggggtgt
     2461 gctcgcggca ccaacggtgg ctgggcgtgg cgatcaagcg cgcaagaaat atcgcgttgc
     2521 tgccgtttgc gggaagtgcg gcggggacgc agcagcctcg cgcagtggta gcgccggcgc
     2581 ctggagcacc agccggaacg ccgtcgtctt cgcatgcggc aacaccgcca gctgcggcga
     2641 cgccggcggc cgctgctccg acagaagttt ccaacgcata agattcaaga attacaatca
     2701 ggacgaggaa cgggagcgaa gaggaagagc atatgcaaat tattcttcag gaagatatcg
     2761 agaagctggg acaccgcggc gatgttgtga cggtaaagcc aggctacgcg cgtaattttt
     2821 tgttgcctcg caaactggcg gtggaagcca gcgccggaaa tttgaaggcc attgaaaaga
     2881 tccgcggttc gctggcgaaa aagacggcta cggaaatgga agcggcgcag aagcaggctg
     2941 ct
//
LOCUS       DATCBB010000700         1113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_22885, whole genome shotgun sequence.
ACCESSION   DATCBB010000700 DATCBB010000000
VERSION     DATCBB010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22885"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VII37_07810"
     CDS             complement(<1..856)
                     /locus_tag="VII37_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEY5069678.1"
                     /translation="MLDAAMVCGCSQKTVARGCNSEGGAMKRDLYAEVSTRIVAELER
                     GAARWIKPWSATAGMNTPCNAATNRPYSGSNVVLLWMAQTAGYRTPRYLTFKQALDLG
                     GNVRKGEHGTKVYFVKQLQVADQNGKEGETRIVPMMREYTVFNVDQCEGLPDRVLTLG
                     KVKVRNFDERDATIDEFLGSSGVEIREGMGEAYYRPGADFISLPSFEAFKSAATFYAT
                     AFHELGHATGHKSRLNRDLRGRFGEKAYAAEELIAELCAAFLCAEFSLDGELRHAGYI
                     QSWIGLLKA"
BASE COUNT          234 a          338 c          302 g          239 t
ORIGIN      
        1 cggccttgag taagccaatc caggattgga tgtagcctgc atgacgcagc tcgccatcca
       61 gcgaaaactc ggcacagagg aacgccgcgc atagctcagc aatcaattcc tcggcagcgt
      121 atgccttctc gccaaagcga ccgcgcaaat cgcgattaag gcgcgacttg tggcctgtgg
      181 cgtggcccag ctcatgaaac gccgtcgcgt aaaaggtagc tgcgctcttg aaggcttcaa
      241 agctcggcaa gctgatgaag tcggcgccgg gccgataata ggcttcgccc atcccctccc
      301 ggatctcaac tccgctcgaa ccaaggaatt catcgatcgt cgcgtcgcgt tcatcgaaat
      361 tgcgcacctt cactttgccc agcgtcagaa cgcggtcggg caaaccttca cactgatcga
      421 cgttgaacac ggtgtattcc cgcatcatgg gaacgatgcg cgtttcgcct tccttgccat
      481 tttgatcggc aacctgaagc tgcttcacga aatagacctt cgtgccgtgt tcgcccttgc
      541 gcacgttgcc gcctaaatcc aaagcctgct tgaaagtgag atagcgcggt gtccgatagc
      601 cggccgtttg tgccatccac aacagcacga cgttgcttcc tgagtaaggc cgattggttg
      661 cggcgttgca gggcgtattc atgccggccg ttgccgacca aggcttgatc catcgcgccg
      721 cgccacgctc caactccgca acaatgcgcg tcgatacctc tgcatagaga tcgcgtttca
      781 tagcaccacc ctcgctgtta cagccacgcg cgaccgtctt ttgcgaacag ccgcacacca
      841 ttgccgcgtc gagcaaaatt tctaagtttt gcagcgtgat gcgaggctgg cggcctcagt
      901 gggcagggcg cgaccccccg ggtatgctgg acaggccggg tggtgtgggt gccgcgtaac
      961 aggtgagctg aaattttgta ggaaccaaaa accaaatctt taataaatta taaacttcgc
     1021 ggttgccctc tgcgtctccg cgtagcgctt ggcaaggttg actattctgt gccgcagata
     1081 gccatgccga taggtgccgt accgctcacg tcg
//
LOCUS       DATCBB010000701         1418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_55539, whole genome shotgun sequence.
ACCESSION   DATCBB010000701 DATCBB010000000
VERSION     DATCBB010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55539"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..>1418)
                     /locus_tag="VII37_07815"
     CDS             complement(142..>1418)
                     /locus_tag="VII37_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5069679.1"
                     /translation="EVGKCGVAISSLADMEVLFDKIPLANVTTSMTINSPAAIIWAMY
                     LAVAEQQGADWKKLSGTLQNDILKEYIAQKEYIYPPEPSMRLVIDTFEFGVKNTPKFN
                     PISISGYHIREAGSTAIQELAFTLRDGMEYVEWGMRRGMDVDAFVPQLSFFFNAHNDF
                     FEEIAKYRAARRIWHKAMVERYKSKNPRSWALRFHTQTAGCSLTAQQPYNNVVRTAIQ
                     ALAAVIGGTQSLHTNSLDEAWALPTEFAATLALRTQQIIAHETGVANTVDPLGGSYFV
                     ETLTNEVERGAWDYIHKIDAMGGMVTAIERSYPQREIAEASYRYQMEVDRKEKIVVGV
                     NDFVTKENPLDILQIDETVADRQAARLKKLRADRSQAEVDRRLNTLRTAARGKENLMP
                     FIYDTVKAYATLGEICEAMRDVFGTYEEVAIT"
BASE COUNT          258 a          328 c          475 g          357 t
ORIGIN      
        1 tagatgacct ccatgcctgc gtcgcggagg gcgcgggcga taatttttgc gccgcggtcg
       61 tgaccatcga ggccgggctt ggcaattaag acgcggattt tttttgtggg catggtttgt
      121 ttagcttggc acggttgcta attacgtgat ggctacttct tcgtaggtgc cgaagacgtc
      181 gcgcatggct tcgcagattt cgccgagggt ggcgtaggct ttcacggtgt cgtagatgaa
      241 gggcatcagg ttttctttgc cgcgggctgc ggtgcgcaag gtgttgaggc ggcgatcgac
      301 ttcggcttgg gagcgatcgg cgcggagttt tttgaggcgt gcggcctggc ggtcagcgac
      361 ggtttcgtcg atctgcagaa tatctagcgg gttttcctta gtgacaaaat cgtttacgcc
      421 cacgacgatt ttttctttgc gatcgacttc catttgatag cggtaggagg cttcggcgat
      481 ttcgcgttgc gggtagctgc gttcgatggc ggtgaccatg ccgcccatgg cgtcgatttt
      541 atggatgtaa tcccaggcgc cgcgctcgac ttcattggtg agggtttcga cgaaatagga
      601 gccgccgagc gggtcgacgg tgttggctac gccagtttcg tgggcaatga tttgttgcgt
      661 gcgcaaggcg agagtagcgg caaattcagt ggggagcgcc caggcttcat cgagggaatt
      721 ggtgtgcagc gattgcgtgc cgccgattac tgcggcgagg gcctggatcg cggtgcgcac
      781 tacgttgttg tagggctgct gcgcggtaag ggaacagccg gcggtctggg tatggaaacg
      841 cagtgcccag gagcgcggat tttttgattt gtaacgctcg accatggctt tgtgccagat
      901 gcggcgagcg gcgcgatact tggcgatttc ctcgaagaaa tcgttgtgcg cgttaaagaa
      961 gaaagaaagt tgcgggacga acgcatccac gtccatgccg cgacgcatgc cccactcgac
     1021 gtactccatg ccatcgcgca gagtgaacgc caattcctga atggcggtgg agccggcttc
     1081 gcgaatgtga tatccgctga tggagatggg attaaatttg ggagtatttt ttacgccgaa
     1141 ttcaaaagtg tcgatgacca ggcgcatgga aggttcgggc gggtagatgt attccttctg
     1201 cgcgatgtat tccttgagga tgtcgttctg caaagtgccg gagagttttt tccagtccgc
     1261 gccttgttgt tcggccacgg cgagatacat cgcccatatg atggcggcgg gcgaattgat
     1321 ggtcatggag gtggtgacgt tggccagcgg aattttgtcg aagagcactt ccatgtcggc
     1381 gagtgaactg atagccacgc cgcattttcc gacttcgc
//
LOCUS       DATCBB010000702         1074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_314361, whole genome shotgun sequence.
ACCESSION   DATCBB010000702 DATCBB010000000
VERSION     DATCBB010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314361"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..865
                     /locus_tag="VII37_07820"
     CDS             137..865
                     /locus_tag="VII37_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY5069680.1"
                     /translation="MNPNAAIVEKHKTAQTGAVGCDDLLHRSLYHVQDGIRIFCGDNR
                     KLAPMLGEYDLLCTDPPYGIGEGNQKRILSRANAAKAIDYGHTDWDSTPPEPWMLQMA
                     MAKARWQIIWGGNYYGLPAAKCWLVWDKENGENDFADCELAWTNLDMAVRRIKYRWAG
                     MLQANMAEKDVRYHPTQKATSVMEWCIGKVPDAKTVLDPWMGSGTTLIAARAKGLQAD
                     GIEINEQYCAAAVARLSQGVLLAV"
BASE COUNT          274 a          293 c          327 g          180 t
ORIGIN      
        1 agtagcgcgt ggactgtgcg aaacgtatcg gaatgaaccc gtttcaaggt ttgaactgcc
       61 gcaactggct gaaaagacag agccacgcga acccgacgac tcaaatttct aacgatgaat
      121 ccagacaaag cgcccaatga accctaacgc cgccatcgtc gaaaagcata agaccgccca
      181 aactggcgct gttggctgcg atgatttgtt acaccgcagc ctctaccacg tacaagatgg
      241 aatccgaatc ttctgcggcg acaatcgaaa gctcgcgccg atgctcggtg aatatgacct
      301 gctctgcacc gacccgccgt atggtatcgg cgagggcaac cagaaacgaa tcctcagtcg
      361 tgctaatgcc gcgaaagcga tagactacgg gcacacggat tgggactcga cgccgcctga
      421 accgtggatg ctccaaatgg cgatggcaaa agcacgctgg caaatcatct ggggcggaaa
      481 ctactacggc ctgcccgccg cgaaatgctg gctcgtgtgg gacaaggaaa acggtgagaa
      541 cgactttgcg gactgtgagc ttgcgtggac gaacctcgac atggcggtgc gccgaatcaa
      601 ataccgctgg gctgggatgc tccaagcgaa catggccgaa aaggacgtgc gctatcaccc
      661 gacgcaaaag gcaacgtccg taatggaatg gtgtatcggc aaggtgcccg atgcaaaaac
      721 cgtgctcgac ccgtggatgg gaagcggcac gacactgatc gccgcccgcg ccaaagggct
      781 gcaggcggac ggaatcgaaa tcaacgaaca atactgtgcc gctgcggtag cccggctatc
      841 ccagggcgtg ctgctggcgg tgtaacgccc cgagctatgc agcaccccgg aaggacaact
      901 aactttatga aatgcgaaaa atgtggtgga gcgatgaatc tggtcgaagc gtggcattgg
      961 gacggcaagc gtcatggaca tcttgtttgt cctgaagggg cggagcgcag cggagtcctg
     1021 caaggatgtc tgccatcagc gacgggttcg gctgacgcgc gagcggcgga cgaa
//
LOCUS       DATCBB010000703         6361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_443745, whole genome shotgun sequence.
ACCESSION   DATCBB010000703 DATCBB010000000
VERSION     DATCBB010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443745"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VII37_07825"
     CDS             <1..1099
                     /locus_tag="VII37_07825"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5069681.1"
                     /translation="GTVKGPVVGIAVEKVEDLQQYKGKLKGAVVVLGKPLDLQAPQNP
                     MLTPWGEETIPIAFPKTDKPFDFTAYMKLRTAELAFFAEEKAAAVLLGSEKWFGLLNM
                     SISGRNYQPGLIPTAYIAREDFTLLWRLLDSGAVQAEVNIGGNFSGKPAEVYNTVAEI
                     PGTEKPDEVVIIGAHLDSWDLGTGATDNGTGSTAVLEAARALKKSGVKPKRTIRFVLF
                     TGEEQGLNGSKTYVTMHQDELKKISAVLVHDSGTGKVLTIGLMGNYDAREIIDRAMYP
                     LARAKEIGLAEPTLRKEDGSDHVPFDEAGVPGFWCVQEVADYDKTHHSQADTVDRVRW
                     DDLTEGAQVLAIFAYNVAQLPELLPRKSPKP"
     gene            1147..1596
                     /gene="dtd"
                     /locus_tag="VII37_07830"
     CDS             1147..1596
                     /gene="dtd"
                     /locus_tag="VII37_07830"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY5069682.1"
                     /translation="MRAVLQRVSRARVTVEERTVGHIAHGLMIFLGVGAADTSSTAAS
                     MAEKIANLRIFEDDAGKMNRSLLDVKGSAMVVSQFTLYGDARGQRRPSFIAAARPEQA
                     KILYEEFCSALANLNIPVQTGIFQATMSVELVNEGPVTILLDSDKTF"
     gene            1641..2117
                     /locus_tag="VII37_07835"
     CDS             1641..2117
                     /locus_tag="VII37_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5069683.1"
                     /translation="MATKKKQINSAAKQAGIKIRRAETSDAARIAELSGQLGYPATPA
                     EIAQRLRNIKPTSHHAVLVAESPERKVIGWLHVSVSPLLETQLRAEVNGLVVTDEERS
                     RGTGALLLSTAEHWARKRGCKSMSVRSNVIRDRAHQFYLRHGYEHYKTQKAFRKSI"
     gene            2250..2795
                     /locus_tag="VII37_07840"
     CDS             2250..2795
                     /locus_tag="VII37_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5069684.1"
                     /translation="MKAAISDTEYRALAELRYRIRHFIQKGDAAAQRASLEPQQYLML
                     LAIRGLPKGAVATIRTLAERMALKHHSAVELIDRLESHGLVRRSRSPGDKREVRVSLL
                     PRGSKLLERVARERLSELKSSGMALADAITALVERKHPLHKITQINQLTDPQRRLAIR
                     SKTVRRKRKTILTPFERKRGA"
     gene            2830..4641
                     /locus_tag="VII37_07845"
     CDS             2830..4641
                     /locus_tag="VII37_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5069685.1"
                     /translation="MAIVTIPNPSDITAAPPGRTPWWHAPGRQWRAFRDQREEQVFLV
                     LTLLIGAIVGLIVVAFILITERFGARLYPVGAATWRRIFVPVAGSLAMGYLLYRFFPD
                     ARGSGVPQTKAALWARGGRISFATVFGKFFCTSATLACGIPLGREGPAVQVGSGIASM
                     LGRYLGLKPEKVKALIPVGAAAAIAAAFNTPLAAVVFALEEVVGDLHAPVLGSVVLAS
                     ATSWGMLRLLLGNDPLFQVPQYQLVHPGELGIYALLGVLGGFISAAFTRLLLWMRAWF
                     LRRPKKTVWFQPLAGGLTVGVMACFVPQILGVGYKYVGDVLNGGMALKLMFLLLVLKL
                     FAVTISYASGNAGGIFGPSLFLGAMLGGIVGTLAHHFAPGYVATPGAYALVGMGTAFA
                     GIVRAPMTSVLMIFEITRDYAVIVPLMISNLISFFISSRFQREPIYEVLAHQDGIHLP
                     TAGSRIQDAQRQVYQAMHAATEVLETRATIQESLNKVASSRLHAWPIVDQSGVIGMIS
                     LSRLQKALTEGETEKPLSDILKDSEFPHVHMDHSLNMALDRMGSSQLDVLPVVNRANV
                     HKLEGIVTLQDVLRLYGVGPSETTVASGESATTPPQH"
     gene            complement(4680..>6361)
                     /locus_tag="VII37_07850"
     CDS             complement(4680..>6361)
                     /locus_tag="VII37_07850"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY5069686.1"
                     /translation="GVASASGKELRIENFDAHIVIGGNGTIDVTETIRAHFIGTNWHG
                     LYRSIPVEYVTPQGLNYTLFLDVKQVTDGSGHRLKFDSNRVRHYRKLKIYIPDPDNSV
                     QTISIEYTISDAIKFFEDHDELYWNVTGDEWDVPIQSARANIILPAGSSNIRATAFTG
                     VYGSRAKDAEIDVAGNGVEVQTTAPLPYHAGLTVAVAMDKGAIREPSVADLVGRFLRS
                     NWPLFIPIIVFCVTFYLWWTRGRDPRLRPIAAQYEPPNQLTPGEAGTLVDDSANMRDI
                     TASIVDLAVRGYMVIEEVQKDRMLGLMHEKDYNFILQKDRAQWSALKTHEQALLDGFF
                     TMGTAGESISMDSLQNRFYKTLPVMRIGIFDSLVSQGFYKRRPDSVRSTYIGAGVVVG
                     MLMIWGGGAMARALGIAPLPFIVGGVISALVICAFGWFMPAHTEQGTRALENVLGFED
                     FLNHVEADRFNRTIKTPQMFEKFLPFAMAFGVEKNWSKAFDGIMTQPPGWYRGGSGPI
                     FYPVNFTNNLNSMSSRASTVMASAPRSSGGSGFVGGGSSGGGFGGGGGGGF"
BASE COUNT         1520 a         1855 c         1643 g         1343 t
ORIGIN      
        1 gggaaccgta aaaggaccag tagtaggcat agccgtagaa aaagtcgaag acctgcagca
       61 atacaaaggc aagctaaaag gcgccgtcgt tgttttgggc aagcccctcg atctgcaagc
      121 gccgcagaat cccatgctga caccgtgggg cgaagaaacc attcccatcg ccttccccaa
      181 aactgacaag cccttcgact tcaccgccta tatgaaattg cgcaccgcgg agctggcatt
      241 ttttgccgaa gaaaaagccg cggcagtatt gctcggatca gaaaaatggt ttggcctgct
      301 gaacatgagc atcagcggtc gaaattacca accaggtttg attcccaccg cctacatcgc
      361 cagagaagat ttcacgttgc tgtggcgact gttggattca ggcgcagtgc aagccgaagt
      421 gaacatcgga ggaaatttca gcggaaagcc cgcggaagtc tacaacaccg ttgccgaaat
      481 tcccggcacc gagaagcctg acgaagtcgt gatcatcggt gcgcatctgg attcctggga
      541 tttgggaacg ggcgccacgg ataacggaac cggctcaacc gcggtcctcg aagccgcgcg
      601 cgcactgaaa aaatccggcg tgaaacccaa gcgcaccatt cgcttcgtgc tgttcaccgg
      661 cgaagaacag ggcctgaacg gttctaagac ttacgtcacc atgcatcaag atgaactcaa
      721 aaaaatctcc gccgttctcg tgcacgatag cggtaccgga aaagttttga ccatcggcct
      781 gatgggaaat tacgacgcgc gagaaatcat cgatcgcgcg atgtatccgc tcgcccgagc
      841 caaagaaata ggcctcgcgg agcctacgct gcgaaaagaa gacggctccg atcacgtgcc
      901 attcgatgaa gccggagtgc ccggcttttg gtgcgtgcaa gaagtagccg actacgacaa
      961 aacgcaccat tcgcaagccg acaccgtcga tcgcgtgcgc tgggatgact tgacggaagg
     1021 cgcgcaggtg ttggccatat ttgcgtacaa cgtagcccag ttgcccgaat tattgccgag
     1081 aaaatcgccg aagccataag gacagggccg ctgccgttcg aatgaccaag gagatggcca
     1141 caatttatgc gagcggtatt gcaacgcgtc agccgcgccc gcgttacagt tgaagaacgg
     1201 accgtaggcc acatcgcgca cggcctcatg atttttctcg gcgtgggcgc cgcggatacg
     1261 tcgtcgacgg ccgcttcgat ggccgaaaaa attgcgaatt tgcgcatttt tgaagacgat
     1321 gcaggcaaga tgaatcgctc tctactcgat gtaaagggta gcgctatggt cgtttcccaa
     1381 tttacccttt acggagacgc gcgcggccaa aggcgtccaa gctttatcgc cgccgctcgc
     1441 cctgaacaag caaaaatatt atatgaagag ttttgttccg ccttggcgaa tttgaatatc
     1501 ccagtgcaaa cagggatttt tcaagccacc atgtccgtgg aattggtcaa cgaagggccc
     1561 gtaaccatct tgctggattc cgacaaaacc ttttgaccgg ttggaatttc aggccgaaaa
     1621 taaattaggc attgccaacc gtggcgacca agaaaaaaca aatcaattcc gccgccaagc
     1681 aagcaggaat aaaaatccgc cgcgccgaga ccagcgacgc cgcacgaatc gccgagcttt
     1741 ccggccaatt gggttatccc gccacacctg cagagattgc ccagcgattg cgcaacatca
     1801 agccgacctc gcatcacgcc gtgctggtcg ccgaatcacc tgagcgaaag gtcatcggct
     1861 ggttgcacgt cagcgtctct cctctgctcg agacacaact gcgcgccgaa gtgaatggat
     1921 tggtagtgac cgacgaggaa cggagccgcg gcaccggcgc ccttctctta agcaccgcgg
     1981 aacactgggc gcgtaaacgc ggatgcaaga gcatgtccgt gcgctccaac gtaatccgcg
     2041 accgggctca tcagttttac ctgcgccacg gctacgagca ctacaaaaca caaaaggcct
     2101 ttcgcaaatc catctgacta tcttacgaag tgaacccggt ccagtttgcc ggcattaaac
     2161 ccataagctc accgccgtaa ccaattcccc atgcgatgag actggtcagt tggcatgata
     2221 tatcgtatta cgatatactt ttgatagcga tgaaagctgc tatctcagac acggaatatc
     2281 gcgccctcgc ggaacttcgc taccgcatac gccatttcat ccagaaaggc gatgccgcgg
     2341 cccagcgcgc aagtctcgaa ccccagcaat acctgatgct cctcgccatt cgtggtttac
     2401 ccaagggcgc tgtggccacc atacgcacct tggccgagcg catggccctc aaacatcaca
     2461 gcgccgtcga attgatcgac cgcctcgaat cgcatggctt ggtgcgcaga agccgcagcc
     2521 caggcgacaa acgagaagtg cgagtgtcgc tgctgccacg tggatcaaag ctgctggaac
     2581 gcgtcgcccg cgagcgcctc agcgaattaa aatccagcgg catggcgcta gccgatgcca
     2641 tcacggcact cgtggaacgc aaacacccgc tacacaaaat aacccaaata aaccagctga
     2701 ccgacccaca acgtcgacta gcaatacgca gcaaaacggt aaggcgaaaa cgaaaaacga
     2761 tacttacgcc gttcgagaga aaaaggggcg cttaagaatt ccttcgcgca ggctgaatat
     2821 atttctaata tggctattgt gactatcccc aaccccagcg atataactgc ggctcctccc
     2881 ggccgcacgc catggtggca tgcgcccggc aggcaatgga gagccttccg tgaccagcgc
     2941 gaggaacagg tcttcctcgt tctaacactt ctcatcggcg cgattgtcgg tttaattgtt
     3001 gtagctttca ttttaatcac cgaacgcttc ggcgcgcgcc tatatccagt gggtgccgcg
     3061 acgtggcgca gaatttttgt cccagtcgcc ggttccttgg ccatgggata tctcctgtat
     3121 cgattttttc ccgatgcgcg cggcagcggc gtcccacaaa cgaaagcagc gctgtgggct
     3181 cgcggaggac gcatttcttt tgcgaccgtc tttggaaagt ttttttgtac ttcggcgacg
     3241 cttgcttgcg gaattcccct ggggcgcgaa ggacccgccg tgcaggtggg ttcaggcatc
     3301 gcctccatgc tgggccgata tttaggattg aaaccggaaa aggtaaaggc gcttattccc
     3361 gtaggcgccg ccgctgcgat cgctgcggca ttcaacaccc cgcttgcagc agtagtcttt
     3421 gcactggaag aagtagtagg cgacttacac gccccagtat tgggttccgt agtgctcgct
     3481 tccgcaactt cctggggaat gttgcgattg ctgctcggga atgacccgct ctttcaagta
     3541 ccgcaatacc agctcgtgca cccaggtgaa ctcggaattt atgcgctcct cggagtcctg
     3601 ggtggcttta tctctgctgc atttacccgg ctacttttgt ggatgcgcgc gtggttcttg
     3661 cggcggccga aaaaaacggt gtggttccag ccgctggcgg gcggtttgac cgtcggggtc
     3721 atggcctgtt tcgtgccgca gatactgggc gtcggctata aatacgtcgg cgacgtctta
     3781 aacggcggca tggcgctgaa attaatgttc cttctgctgg tcctcaagct atttgcagta
     3841 accatcagct atgcctccgg aaatgccggt ggaattttcg gcccaagctt gtttctagga
     3901 gccatgctcg gcggaattgt aggaacgttg gcgcatcact tcgcacccgg ttatgtcgcc
     3961 acgcccgggg cctacgcact cgtgggcatg ggcacagcct tcgccggaat tgtgcgagcc
     4021 ccgatgacct ccgtgctcat gatttttgaa attacccgcg actatgccgt gatcgttccc
     4081 ctgatgattt ccaatctcat cagctttttc atttcttcgc gctttcaacg agagcctatt
     4141 tacgaagttt tggcccacca ggatggcatt catttaccaa ctgccgggtc gcgtatccag
     4201 gacgcccagc ggcaggttta ccaggccatg cacgcggcta cagaggtttt ggagacacgt
     4261 gccaccattc aagaatctct aaataaagtt gcctccagcc gccttcacgc ctggcccatc
     4321 gtcgatcaaa gcggcgtgat cggaatgata agtctcagcc ggctgcaaaa agcccttacc
     4381 gagggagaaa cagaaaaacc gctcagcgac attctgaaag acagcgaatt tccccacgtg
     4441 cacatggacc attctttgaa catggcgctt gatcgcatgg gttctagcca gctcgacgtt
     4501 ttgccggtag tcaatcgcgc caacgtgcac aagctcgaag gaattgtcac gctgcaagat
     4561 gtcttgaggc tctacggcgt ggggccaagc gaaacaaccg tggcttctgg cgagtcagcc
     4621 acaacgccac cgcagcatta atgcgattgc gcgaagcgca gtgctaaatt gagaacgacc
     4681 tagaacccgc ctccaccgcc gccgccgaaa ccgccgccag aagatcctcc gcctacaaaa
     4741 ccagaaccgc ccgagctgcg cggcgcagac gccatcactg tgctcgcgcg cgacgacata
     4801 ctgttaaggt tgttggtaaa atttaccgga taaaaaattg ggcccgagcc gccgcgatac
     4861 caaccgggcg gttgcgtcat gattccatca aaagctttgc tccaattttt ctccaccccg
     4921 aacgccatcg cgaaaggcag aaatttctca aacatttgtg gagtctttat cgtgcgatta
     4981 aaacggtctg cttccacatg atttaaaaag tcctcgaagc ccagcacgtt ttctaacgcg
     5041 cgcgtccctt gctccgtgtg cgccggcata aaccagccaa atgcacagat caccaatgcc
     5101 gagatgactc caccaacgat gaatggcagc ggcgcgatgc ccagagcgcg cgccatggcg
     5161 ccgccgcccc atatcattaa cattccaacg acgacgcctg cacctatata tgtagagcga
     5221 acactgtccg gccgccgctt ataaaaaccc tgcgacacca gtgagtcgaa aattccgatc
     5281 ctcataactg gcaaagtctt gtaaaagcgg ttctgcagac tatccatgga aatagattcg
     5341 cccgccgtac ccatggtaaa gaagccgtcc agaagcgctt gttcatgtgt cttaagcgcg
     5401 ctccactgcg cgcgatcctt ttgcaatatg aagttgtagt ccttctcgtg catcagcccc
     5461 agcatgcggt ccttctgcac ttcttcgatg accatatacc cgcgtactgc gagatccaca
     5521 atagaagcgg tgatgtcgcg catattcgcg gaatcatcca ccagcgtgcc tgcctctcca
     5581 ggcgtgagtt gattgggtgg ctcgtattgc gctgcgatgg gacgtagccg gggatcccgc
     5641 ccgcgcgtcc accacaaata aaacgtcaca cagaaaacaa tgatcggtat gaacagcggc
     5701 cagttactgc gcaaaaacct tccaaccaga tctgcaacgc taggctcacg aatcgcgcct
     5761 ttatccatcg ccaccgccac ggtcaatcct gcgtggtaag gaagcggcgc agtggtctgc
     5821 acttcgacgc cgttacccgc gacatcaatt tccgcgtcct tggctcgtga accgtacact
     5881 ccggtaaacg ccgttgctcg aatattactg gaccctgccg gcagaataat atttgcgcgc
     5941 gccgactgaa tgggcacatc ccactcgtcg cccgtcacat tccaataaag ttcgtcatgg
     6001 tcctcgaaaa atttaatcgc gtcgctaatc gtgtattcga tggaaatcgt ttgtaccgaa
     6061 ttatccggat caggaatgta gattttgagc ttgcggtaat ggcggacccg gttggaatcg
     6121 aatttgagcc gatgtccact cccatcggtc acctgtttca catccaggaa caacgtgtaa
     6181 ttcaagccct gcggcgtcac atactccaca ggaatagaac gatacagccc gtgccagttg
     6241 gtaccgataa aatgcgctcg tatcgtctcg gttacatcga tcgtgccatt gccgccgatc
     6301 acgatgtgcg catcaaaatt ctcaatacgc agttctttgc cgctggcact ggccacgcca
     6361 g
//
LOCUS       DATCBB010000704         1245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_184172, whole genome shotgun sequence.
ACCESSION   DATCBB010000704 DATCBB010000000
VERSION     DATCBB010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184172"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VII37_07855"
     CDS             complement(<1..585)
                     /locus_tag="VII37_07855"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY5069687.1"
                     /translation="MKNSKKSWLLSACALALLLALAVPHAAAQNVGTIRGQILDVSGK
                     PWAEIGIQAVNDQGAKVDTKTDKDGNYTFHNLKSGVYSVFVQLPAPNPPYEVKTQVQS
                     GGEARVDLNFKDIVAKQGSQYAEQAKKQEEEKSKFEGMKAHFAAGNALLDQERKAKED
                     ALKAPADQRDAAKAKVVDLSNQAITELDAAQKATG"
     gene            complement(683..>1245)
                     /locus_tag="VII37_07860"
     CDS             complement(683..>1245)
                     /locus_tag="VII37_07860"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEY5069688.1"
                     /translation="RQAFEAIADFYGITERLVVQLDHFRAIGGSALTDQNIRVPENQQ
                     PSSSAEIPVTYVPFRNAHFLSVAVSWAEVIGAHSIYIGAVAEDSSGYPDCRPEYYRVF
                     QELIRVGTRPETSIEIVTPVITLKKSAIIRKGLELGAPLHLTWSCYQDDASACGVCDS
                     CVLRLRAFAEAGLADPISYRPLAAQP"
BASE COUNT          234 a          342 c          377 g          292 t
ORIGIN      
        1 accagtcgct ttctgcgcgg catccaactc cgtgatcgcc tggttggaca ggtccacaac
       61 cttcgctttg gccgcgtcgc gctggtcagc gggagccttt agtgcgtcct ccttggcttt
      121 gcgttcctgg tccagcaacg cattgccagc ggcgaagtgc gccttcatgc cttcaaactt
      181 ggacttctcc tcttcctgtt tcttggcctg ctcggcgtac tgcgaacctt gcttggcaac
      241 gatatcttta aaattaaggt ctacgcgcgc ttcacctccg ctttgcacct gggtcttcac
      301 ttcgtatggc gggttgggag cgggaagctg cacgaatacg ctgtaaacgc cgcttttgag
      361 attgtggaac gtgtaattgc cgtctttatc tgttttcgtg tctaccttcg cgccctgatc
      421 gttgaccgcc tgaattccga tttccgccca aggtttgccg ctgacgtcca ggatttgtcc
      481 gcggatcgtg ccgacgtttt gcgccgcggc gtgaggaacc gcgagggcca gcaacagcgc
      541 cagcgcgcag gcgctcagca accaggattt cttggaattc ttcatactcg attctccttt
      601 tgcacggcaa cttcttggac ggcgacttgc gagccggacg agcggggacc attatcacta
      661 catttgatgc cggattcaaa gctcaaggct gcgcagccag gggccggtag gagatagggt
      721 cggccaaccc ggcctccgcg aaggcgcgca atcgtaacac gcaactatcg catacgccgc
      781 aggcggaagc atcatcctga tagcacgacc aggtcagatg gaggggcgcg cctagctcga
      841 ggcctttgcg aatgatggcg ctttttttga gggtgatgac cggcgtgacg atttcgatgc
      901 tggtttccgg gcgcgtgccg acgcgaatca attcctgaaa tacgcgatag tattccgggc
      961 ggcaatcggg atatccggag ctgtcttcgg cgactgcgcc gatgtagatg gagtgagcgc
     1021 cgatgacttc agcccaactg acggccacgg aaagaaaatg ggcgttgcga aaaggcacgt
     1081 aggtgacggg aatttcagcg gaggaggagg gctgctgatt ttcggggact cttatatttt
     1141 ggtcggtgag ggccgagcct ccgatggcgc gaaaatgatc gagctggacg accaggcgct
     1201 ctgttatgcc gtaaaaatcg gcgatggctt cgaaggcttg gcgct
//
LOCUS       DATCBB010000705         1057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_314399, whole genome shotgun sequence.
ACCESSION   DATCBB010000705 DATCBB010000000
VERSION     DATCBB010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314399"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>1057
                     /locus_tag="VII37_07865"
     CDS             40..>1057
                     /locus_tag="VII37_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5069689.1"
                     /translation="MKTFPSTLTTTSERLTNVLSYGALLLLGYVVFLIFEPFLVPLTW
                     SAVLAIFFYPVHERLAKRLKPNYAAMMSTVGVTVLLIAPTLVVLSYTAKQAIDATARL
                     QTALMNPERELPAEVVARVRAQLPEAWQSTDFSEPLRQGAEKVATFLAGKFGALVKNL
                     LSFFVALFILVFALFFMFRDGHDVVRAIKHLFPFDRTIQEEMLMESKELIFASVAVGL
                     LVAGIQGFLGGTAFAITGLHSPVFWGVVIAFFSLVPVVGSAMIWLPTALWLGFSGHWG
                     KALTIMIICGAVAGIADNIVRPLLLRNRTQLNELLLFIGVLGGLSVFGLLGLVVGPAI
                     VSAAM"
BASE COUNT          193 a          307 c          294 g          263 t
ORIGIN      
        1 taaccacttc ccctcaaatt gcggcagaat agaccccgca tgaagacttt tccctccacg
       61 ctcaccacca ccagcgagcg cctcaccaac gtactttcgt acggagcgct gctgttgctc
      121 gggtacgtgg tgtttctgat cttcgaaccg tttctggtgc ccctgacgtg gtccgcggtg
      181 ctagctatat ttttctaccc ggtgcacgag cgcctggcga agcgcttgaa gcccaactac
      241 gcggctatga tgagtaccgt tggtgtgacc gtgctactca tcgcgccaac actggtagtg
      301 ctctcctaca ccgcgaaaca ggccattgat gccaccgcaa gattacaaac cgcgctcatg
      361 aatcctgaac gcgaactgcc cgccgaagtt gtggcgcgag tgcgcgcgca gcttccagag
      421 gcctggcaat cgaccgattt ttcagagccc ttgcgacagg gtgccgagaa agtcgcaacc
      481 ttcctcgccg gcaaattcgg agcgctggtt aagaatctgc tgagtttttt tgtcgccttg
      541 ttcatcctgg tattcgcgct gttctttatg tttcgcgacg gccacgatgt agtgcgagcc
      601 ataaaacact tatttccatt tgaccgcacc atacaggaag agatgctcat ggaatccaag
      661 gagctgatct tcgccagtgt ggcagtggga cttctcgtcg cgggcatcca gggatttctg
      721 ggcgggacgg cgttcgcgat caccggcctg cactcaccgg ttttctgggg agtggttatc
      781 gccttttttt cgctggtacc ggtagtgggt tcggcgatga tctggctgcc caccgctctc
      841 tggttgggat tcagcggaca ctggggaaaa gcccttacca tcatgatcat ttgtggcgcg
      901 gtcgcgggca tcgccgacaa catcgtgcgc ccattgttac tgcgtaatcg cacgcagctc
      961 aacgagctac tcctgtttat tggcgttctg ggaggacttt cggtattcgg cttacttggc
     1021 ctggtggtcg ggcccgccat tgtgtccgcc gcgatgg
//
LOCUS       DATCBB010000706         1620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_478770, whole genome shotgun sequence.
ACCESSION   DATCBB010000706 DATCBB010000000
VERSION     DATCBB010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478770"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VII37_07870"
     CDS             <1..554
                     /locus_tag="VII37_07870"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY5069690.1"
                     /translation="LGLLLLHPDTTYHVRELARLTGTSAGTLHKELTKLTNGGVLRRQ
                     EVGNQVRYSADRDCPIFEELASILRKTSGLVNVLVDALSSIEKNITLAFVFGSMARGE
                     QQSNSDVDVMLVGSLGFADAVQVLHPVQATLQREINPVVYSLEEFRRRAASDDSFVRE
                     VLAEPKLFVVGNENELRKLTQDQ"
     gene            523..963
                     /locus_tag="VII37_07875"
     CDS             523..963
                     /locus_tag="VII37_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069691.1"
                     /translation="MSLENLLKINKLQTHNPSRESLQRLLQAAARNIADARIAEISSE
                     TRFDAAYKCIMQCAMVALWANGYRTSTSQPGHHQVAIQTLPITIGLEQGTVIVLDALR
                     KQRNINDYEGDPISDQAVIECIKQAEQLYKVVSAWLATNRPDLA"
     gene            1156..>1620
                     /locus_tag="VII37_07880"
     CDS             1156..>1620
                     /locus_tag="VII37_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5069692.1"
                     /translation="MQNFSSADAQLDQIKLPPHSVEAEQSVLGGLLLEASALDKITDL
                     MTADDFYRQEHRLIFRQIVRLSEQAKPVDVITVAEALEIAGELDKVGGLAYLGGLAQN
                     VPSAANIRRYGEIVRERSIMRKLAEVGTDIASSAYNPTGRDAAQLLDEAETKV"
BASE COUNT          383 a          393 c          477 g          367 t
ORIGIN      
        1 tgcttgggct attactgctg cacccagaca caacctatca tgtgcgtgag ttggcgcgcc
       61 tgacgggcac gtctgccggt accttgcata aagaattgac caagctaacc aatggcgggg
      121 tgttgcggcg gcaagaggtg ggaaatcagg tgcgctatag cgcagatcgc gattgtccga
      181 tttttgagga gttggcgagc attttgcgca agacttcagg gctggtgaat gtgctggtag
      241 atgcattaag cagcattgaa aaaaacatta ccttggcttt tgtgtttggc tcgatggctc
      301 gtggcgaaca gcaatccaac agcgatgtgg atgtgatgct ggtgggcagc ttgggctttg
      361 ccgatgctgt gcaggtgttg catcctgttc aggcgacttt acagcgcgag atcaatcccg
      421 tggtgtattc gttggaggag tttcgtcgtc gtgccgccag tgatgattcg tttgtccggg
      481 aagtgttagc cgagcccaag ctgtttgtag tgggcaatga aaatgagctt agaaaactta
      541 ctcaagatca ataagctgca aacgcacaat ccgtcgcgcg aatcgctgca gcgattgttg
      601 caagccgcag ctcgcaatat tgcggacgcg cggattgcag agatcagcag cgagacacgt
      661 ttcgatgcag cctataagtg catcatgcaa tgtgcgatgg tggcgctatg ggcgaatggc
      721 tatcgcactt ctaccagtca gccggggcat catcaagttg cgattcaaac gctgccaatc
      781 accatcgggt tggaacaagg cacggtgatt gtgctggacg cattgcgcaa gcaacgcaat
      841 atcaacgatt acgagggcga cccaattagc gatcaggcgg tcatcgagtg tatcaaacag
      901 gcggagcaac tttacaaggt agtatctgcc tggcttgcaa ctaaccggcc tgatttggca
      961 tgagagaaaa gctggacgga accaaatgta ggtcgggctt aaccagcggc ttatgtcacc
     1021 gttttttggt gacttagccg aatggctatg caacgcaaca caaaaaggac tggcaacagt
     1081 ccttttttgt ttttaggctt tcgcctatta caaggtaaaa tagcccccgc tcaaaagccc
     1141 ctgtgaatta atcccatgca aaatttttcc agtgccgatg cacagctcga ccagatcaaa
     1201 ttgccgcccc attcggtgga agccgagcaa tcggtgctgg gtggcttgtt gctggaagcg
     1261 agcgcgctgg acaagatcac cgacttgatg accgccgacg atttctatcg tcaggaacat
     1321 cgcctgatct tccgccagat cgtgcgcctg agcgagcagg cgaaaccggt agacgtgatc
     1381 accgtggccg aggcattgga gatcgcaggt gaattggaca aagttggcgg cttggcttat
     1441 ttgggcgggt tggcgcagaa cgtgccgtcg gccgccaaca tccgccgcta tggcgaaatc
     1501 gtgcgcgagc gttccatcat gcgcaaactg gcggaagtcg gcaccgatat cgccagtagc
     1561 gcctacaatc cgactgggcg cgatgccgcg caattgctcg atgaggcgga aacgaaagtg
//
LOCUS       DATCBB010000707         1570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_347108, whole genome shotgun sequence.
ACCESSION   DATCBB010000707 DATCBB010000000
VERSION     DATCBB010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347108"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VII37_07885"
     CDS             <1..258
                     /locus_tag="VII37_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069693.1"
                     /translation="IKNGAIRRAADTQTKKIGAYSILRKENRDGIKCYLDAPNHGNGA
                     DPWVLFRASGTEPLLRCYAEAAAPELVEEILESGERFVRQG"
     gene            383..1543
                     /locus_tag="VII37_07890"
     CDS             383..1543
                     /locus_tag="VII37_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5069694.1"
                     /translation="MITLKENAPVVADSPESRRYNSTKRWLGVADFLVGFGLLVMLLA
                     TGWSGALRDLAERGASQNYPLAVFLYVLMLMLISKAVGTPLEYYEFRLEHRYNLSNQK
                     LRSWIWDEFKGLVLGLIMGTIVVELLYALMRQTPQHWWIVAWVVFLGLMILLAQIAPV
                     VLFPIFYKFEPLENEELKRRLIVLSERAGTRVRGVYKWHLAEKSKKANAALTGLGATR
                     RIILADTLLDNYSDDEIEAVLAHELGHHVHRHILKSIFVQAGITLFGFWLANEVLRFA
                     VERRNMFETMHDFANLPLLILVSTVLSFLLMPALNAYSRFNERQADRYCFESVATVEP
                     FISSMNKLAEQNLSEKTPSRWVEWLLHSHPAITKRVSAAESWAKSQRKLVIE"
BASE COUNT          343 a          425 c          438 g          364 t
ORIGIN      
        1 atcaaaaatg gagcgatccg tcgcgccgca gatacgcaga ctaaaaaaat cggcgcatat
       61 tccattctgc gaaaagaaaa tcgtgatgga atcaagtgtt atctcgacgc tcctaaccac
      121 ggcaatgggg ccgacccgtg ggtattgttt cgcgcctccg ggaccgaacc gctgctgcgc
      181 tgctatgcgg aagcggcggc gcccgaactg gtggaagaga ttctcgaaag cggcgagcgc
      241 ttcgtgcggc agggctgata aaagagatac gagcttcgag ccgcgagctg cgcgtgcgag
      301 tcaccgcctc gaagcagcgg tgccgagact ttcgtgtact agctagacac gagacacgat
      361 aggataatta caacgcacca gcatgattac tctcaaagaa aacgcacccg ttgtcgccga
      421 ttctccggaa tcgcgccgct ataacagcac caagcggtgg ctgggcgttg ctgatttttt
      481 ggtcggtttt ggcttgctgg ttatgctcct ggctacggga tggagcgggg cgctccgcga
      541 tctggcggaa cgcggcgctt ctcagaatta tccgctcgct gttttccttt acgtgctgat
      601 gctgatgctt atcagcaagg cggtcggcac tccgctcgaa tactacgaat ttcgcctcga
      661 acaccgctac aatctttcca atcagaaact ccggtcgtgg atttgggacg aattcaaggg
      721 actcgtcctc ggcctgatta tggggacaat tgtcgtcgag ttgctctatg cgctgatgcg
      781 ccaaaccccg caacactggt ggatcgtggc gtgggtggtt ttcctcggac tcatgatcct
      841 gctcgcgcag atcgcgccgg tcgtcctctt ccccatcttt tacaaattcg agccgctcga
      901 gaacgaggaa cttaagcgcc gcttaatcgt cctgagcgaa cgcgccggga cgcgggtgcg
      961 aggtgtttat aagtggcacc ttgccgaaaa aagcaagaag gcaaacgccg cgcttaccgg
     1021 gcttggagcg acccgccgca ttattctcgc cgacaccttg ctggataact attcagatga
     1081 tgaaatcgaa gccgtgctgg cgcacgaact cggccatcac gtacaccgtc atatcctgaa
     1141 gagcattttc gtccaggcgg ggatcacgct cttcggcttc tggctggcca atgaagttct
     1201 gcgcttcgcc gtggagcgcc gcaatatgtt cgagacaatg cacgatttcg ccaatttgcc
     1261 gctgttgatt ctggtctcga ccgtgctctc gtttctactg atgcccgcgc tgaacgctta
     1321 ttcgcgcttc aatgagcgtc aagccgatcg ctactgcttc gagtcggtgg ccacggttga
     1381 accatttatt tcgtcgatga ataagttggc cgagcaaaat ctttcggaaa aaactccgtc
     1441 acgctgggtg gagtggttgc tccattcgca cccggcaatc acaaagcggg tttccgccgc
     1501 cgaaagctgg gcgaagtcac agaggaaatt ggtgattgag taatttgtaa tttgataaat
     1561 tgattaatcg
//
LOCUS       DATCBB010000708         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_184235, whole genome shotgun sequence.
ACCESSION   DATCBB010000708 DATCBB010000000
VERSION     DATCBB010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184235"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VII37_07895"
     CDS             complement(<1..904)
                     /locus_tag="VII37_07895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5069695.1"
                     /translation="MSVNHLDPNPVGAPCVMVIFGASGDLTKRKLIPALCNLASDGLL
                     SKQFAIVGFAANDYTTETFRKMLTDEIPKYSGSPIDQKMWNWFTERIYYVKGDFNDVQ
                     AYQRLKQQIEEADKQHNTPGNKFFYLAVAPRFFSPIAKQLGEAGLTKEENGHWARVIV
                     EKPFGHDLESAKQLNQDLKQVLTENQIYRIDHYLGKETVQNLMVFRFSNNIIEPLWNR
                     NYVDHVQITAAETVGVEHRGGFYETAGALRDMVPNHLFQLLTMTAMEPPISFDADEVR
                     NKQAEVLHAIQPFGPEEVLTCMVRG"
     gene            complement(953..1972)
                     /gene="gnd"
                     /locus_tag="VII37_07900"
     CDS             complement(953..1972)
                     /gene="gnd"
                     /locus_tag="VII37_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY5069696.1"
                     /translation="MQLGMIGLGRMGANMVRRLVKNGHQCVVYDRTPDTVKKLASDGA
                     TGSNSLDEFVKKLEKPRAIWLMVPAGVVDATLHDLVPKLEKGDILIDGGNSYYIDDIR
                     RAKELTEKGLHYVDVGTSGGVWGLERGYCQMIGGENDVVKRLDPIFKTLAPGRGNIER
                     TPGREKATGTAEEGYLHCGPSGAGHFVKMVHNGIEYGLMAAYAEGLNILKHANVGKSH
                     RETDAETAPLRNAEHYQYDFNLADVTEVWRRGSVVASWLLDLTAISLLDKPNLEGFSG
                     RVSDSGEGRWTIVAAIESTTPAPVLSAALYQRFSSQGEDDFAGKILSAMRFQFGGHVE
                     KKTAK"
BASE COUNT          434 a          622 c          536 g          518 t
ORIGIN      
        1 gaccgcgaac catgcacgta agaacctctt ccggcccgaa cggctgaata gcgtgaagaa
       61 cctccgcctg tttattgcga acttcgtcgg catcaaagga aatgggcggc tccatggccg
      121 tcatggtcag caactgaaac aggtgattgg gcaccatgtc gcgaagcgcc ccggctgttt
      181 cgtaaaatcc gccgcgatgt tccactccaa ccgtctccgc ggcagtgatc tgcacgtggt
      241 caacgtaatt gcgattccac agcggctcga taatgttatt ggaaaaacgg aacaccatca
      301 ggttctgaac ggtttctttt cccaggtaat gatcgatgcg gtaaatctga ttttccgtga
      361 gcacctgctt gaggtcctgg ttgagctgtt tcgccgattc caggtcatgg ccgaaaggtt
      421 tctcgacaat gacgcgcgcc caatgcccgt tttcctcttt tgttagcccg gcttccccta
      481 gctgcttggc gataggtgag aaaaatctcg gtgcgacggc caggtagaag aatttattac
      541 ccggcgtgtt gtgctgttta tcggcttcct ctatttgttg cttaagccgt tggtaagctt
      601 gaacgtcgtt aaaatcccct tttacgtagt agatgcgttc ggtaaaccaa ttccacattt
      661 tctgatcgat tggcgaaccc gagtatttgg gaatttcgtc cgtcaacatt ttgcgaaatg
      721 tttcggtggt gtagtcgttg gcggcaaaac cgacgatggc gaattgtttg gagaggagcc
      781 cgtcgctcgc caggttgcac agcgcgggaa tcaatttacg cttggtgaga tcaccgcttg
      841 cgccaaaaat caccataacg cagggtgccc ctacggggtt cggatccaga tgattgacgc
      901 tcatgaacga gggccctgcc tttgatttgc acttaccgct gacacttcaa tattatttcg
      961 ctgttttttt ctcgacgtgt ccaccgaact gaaatcgcat ggcggacaaa attttcccgg
     1021 cgaaatcatc ttcgccctgc gaagaaaacc gctggtacag cgcggcactc agcaccggtg
     1081 ccggcgtggt ggactcaata gcggcaacaa tagtccaccg gccttccccg gagtcggaca
     1141 cccggcctga aaaaccctcc aggttgggct tgtccagaag cgaaatggcc gtcaagtcca
     1201 gcagccatga agccaccaca ctgccgcggc gccaaacttc cgtcacgtcc gcaagattga
     1261 agtcgtattg atagtgttcc gcgttccgca aaggcgcagt ctccgcgtca gtttcgcgat
     1321 gcgatttgcc cacgttagca tgcttcaaaa tattcaggcc ttccgcgtac gccgccatca
     1381 gcccatattc gattccgttg tgaaccatct tcacaaaatg gcccgcgccc gacggcccgc
     1441 aatgcagata gccttcttcc gcggtccctg ttgctttttc ccggccagga gtgcgctcga
     1501 tatttccgcg gccaggtgcc agcgttttga aaatcggatc gaggcgcttc accacgtcat
     1561 tttcgccgcc aatcatctgg caatagcccc gctccagtcc ccaaacgccg ccgctggttc
     1621 cgacatccac atagtgcaaa cctttttcgg tcagctcttt tgcgcgacga atatcgtcga
     1681 tgtagtagga gtttcctccg tcgataagga tgtcgccttt ctcgagcttc gggacgagat
     1741 cgtgaagcgt ggcgtccacc acgccagcgg gaaccatcag ccaaatcgcg cgcggcttct
     1801 ccagtttttt tacaaattcg tcgagggagt tactgcccgt cgcgccatcg ctagcgagtt
     1861 tctttaccgt gtcaggtgtc cggtcataga ccacgcactg gtgaccattc ttcaccagcc
     1921 ggcgcaccat gttggcgccc attcttccca gcccgatcat gcccagctgc atcttctcgc
     1981 cctccgcgat agttcctgag gctttgcaaa ttgtttgatt acgcgatggc ctgcttcacc
     2041 gctttcgtca atgtagtcag cccggccgcc acgtctttac cgagatgcac gcgcaacgca
     2101 cggcgcccgc
//
LOCUS       DATCBB010000709         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_120760, whole genome shotgun sequence.
ACCESSION   DATCBB010000709 DATCBB010000000
VERSION     DATCBB010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120760"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="VII37_07905"
     CDS             <1..2099
                     /locus_tag="VII37_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5069697.1"
                     /translation="PHNRALAFHPDYEYAVVDTEKGALLLAADRIAALQSECDIKEAR
                     IRETFKGSEFEGRKFQHPFLPLQVPGILADYVTLDQGSGIVHTAPGHGADDFYSGQKY
                     GLETYAPLDDRGVYLEGLPEYKGKNVFEANPIIVKLLRDRGALLGHHEYKHTYPHCWR
                     CHNPVIFRATEQWFIKMDAIVPGRDKTFRQEALDEVEGVKWIPAWGEERIYEMVEKRP
                     DWCVSRQRFWGVPIIVFYCDACGKRLEDFSALRNVLDWFRKEGADAWYQHTPEELLPP
                     GTKCSCGSIKWRQEHDILDVWFDSGSSNLAVLHGTEWPADIYLEGPDQYRGWFQSSLL
                     VATGIKNAAPFRGVVTHGWTLDEQGRPMSKSLGNVVEPAEICEKWGADLLRLWVASQE
                     YQADVKMSERVMVQLSEAYRKIRNTFRFALGNLANFDPSRDVLPNDQMEEIDRWMLDR
                     TAELSRKCQEWYAGYDFHRVYHAIHDYCVVDLSSFYFDVLKDRLYTKAPRSKSRRSAQ
                     TAVWKITSALVRLLAPILVFTTEEIWKMLPAPTQGASSVHQAEFAEPDSLASGISKDA
                     AEQWTRLAQVRSAVLVALEQARAAKTIGGGLEAKVKLHAGSEAPGLQKLLQERRDVLP
                     ALFIVSQVEVEPNASEAATKSELLPGLTIKIERADGAKCERCWNYSTHVGENPRYPTV
                     CERCTEAMAEIESAAA"
     gene            2202..>2605
                     /gene="lspA"
                     /locus_tag="VII37_07910"
     CDS             2202..>2605
                     /gene="lspA"
                     /locus_tag="VII37_07910"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5069698.1"
                     /translation="MRWFWLTIAVVIADRGTKAWFEASTTESYRHEVVHNLIYLVHST
                     NPGIAFSFFADSNSPWMKSILIAGSIVVVIILTWLLIAGKTGGPMTFAGLALLIAGAT
                     GNVTDRILHGGVTDFLEVWIGSYRWPAFNAADS"
BASE COUNT          599 a          780 c          671 g          555 t
ORIGIN      
        1 ttcctcataa ccgcgcgctc gcttttcatc ccgattacga atacgccgtc gtggataccg
       61 aaaaaggcgc tctgcttctc gccgccgacc gcatcgccgc actgcaatcc gaatgtgata
      121 tcaaggaagc ccgcatccgc gaaaccttca aaggcagcga attcgaaggc cgcaaatttc
      181 agcatccctt cctgccgctt caagtccccg gcattttagc ggattacgtg accctcgatc
      241 agggcagcgg aatcgtccac accgctcccg gccacggtgc cgacgacttt tattccggcc
      301 agaaatatgg tttggaaacc tacgcgccgc tcgatgatcg cggcgtttat ctggaaggat
      361 tgccagagta caaaggcaaa aatgtttttg aagccaaccc catcatcgtc aagttgctcc
      421 gcgatcgcgg cgccctcctc ggccatcacg aatacaaaca cacttacccg cattgctggc
      481 gctgccacaa tcccgtcatc tttcgcgcca ccgaacagtg gttcatcaaa atggacgcca
      541 tcgtacccgg tcgcgacaaa acctttcgcc aggaagccct cgatgaagtc gaaggcgtca
      601 aatggattcc cgcctggggc gaggaacgca tttacgaaat ggtcgaaaaa cgccccgatt
      661 ggtgcgtctc ccgccaacgc ttctggggcg tgcccatcat tgtcttttat tgtgacgcct
      721 gcggaaaacg cctcgaagat ttcagcgcgc tacgaaacgt cttggactgg ttcagaaaag
      781 aaggcgctga cgcctggtat caacatactc cggaagaatt actgccaccc ggaacaaagt
      841 gttcctgcgg ctccatcaaa tggcgtcagg agcacgacat cctcgacgtc tggtttgatt
      901 ccggctcttc caatctcgcc gtcctgcacg gcacggagtg gcctgccgat atttatctcg
      961 aaggtcccga ccaataccgc ggatggttcc agagttcgct cctggtagcc accggaatta
     1021 aaaacgccgc gccttttcgt ggcgtagtga ctcacggctg gactttagat gaacagggcc
     1081 gccccatgtc caaatcctta ggaaacgtcg tcgagcccgc cgagatatgc gagaaatggg
     1141 gcgccgatct tctgcgcctg tgggtggcct ctcaggaata tcaagccgat gtaaaaatga
     1201 gcgaacgcgt aatggtccag ctcagcgaag cctatcgcaa gattcgcaac accttccgct
     1261 tcgcgcttgg aaatttggcg aatttcgatc cttcacggga tgtcctgccg aacgaccaaa
     1321 tggaagaaat cgatcgctgg atgcttgatc gtaccgcgga gttgtcgcgc aaatgccagg
     1381 agtggtacgc cggatatgac ttccatcgcg tatatcacgc catccacgac tactgtgtcg
     1441 tcgatctcag ctccttttac tttgacgtct tgaaggaccg cctctatacc aaagcgcctc
     1501 gcagcaaatc gcggcgctcg gcgcagaccg ccgtctggaa aattaccagc gccttggtac
     1561 gcttactcgc gcccatcctg gttttcacta ccgaagaaat ttggaagatg ctcccggcgc
     1621 ccacccaggg cgcgagcagc gtgcatcagg cggagttcgc cgaacctgat tcgcttgcaa
     1681 gcggaatcag caaagacgcc gccgagcaat ggacacgcct ggctcaagtg cgttcagctg
     1741 ttttggtggc gctcgaacaa gcgcgcgccg ccaagaccat cggcggtgga ctggaggcca
     1801 aagtaaagct tcacgccggt agcgaagcgc caggactaca gaaattgctt caggaaagac
     1861 gcgacgtgct gcccgcgctc tttatagttt cgcaagtgga agtcgaacca aacgcttctg
     1921 aagccgccac gaaaagcgaa ttgcttcccg gactcaccat caaaatcgaa cgcgcagacg
     1981 gagccaaatg tgagcgctgc tggaactatt cgacccatgt aggtgaaaac ccgcgctatc
     2041 caacagtttg tgaacggtgc accgaggcaa tggccgaaat cgaaagcgca gcagcttagc
     2101 aggcgcgatc acgtgacccg cggcatcacc gccaggttcc acagaactgc agaatctgtt
     2161 gccgcgttga aagtgaaagc ttagtgcaaa tttcccaacg attgcgctgg ttttggctca
     2221 ctatcgccgt ggtcatcgcc gaccgcggca ccaaagcctg gtttgaagcc tccacaaccg
     2281 aaagttaccg ccacgaggtc gtgcacaatt taatttatct ggtgcacagc acaaatcccg
     2341 gaatcgcatt tagctttttt gccgattcca attcgccttg gatgaaatca attcttatcg
     2401 ccggctcgat cgtagtcgtg atcatcctca cctggctgtt gatcgctggt aaaaccggcg
     2461 gccccatgac ctttgcgggt ctcgctctgt taattgcggg cgcaaccgga aatgttaccg
     2521 accgcatttt gcacggcggc gtcaccgatt tcctcgaagt ttggatcggc tcttaccgct
     2581 ggccggcttt taacgccgcc gattc
//
LOCUS       DATCBB010000710         1257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_478823, whole genome shotgun sequence.
ACCESSION   DATCBB010000710 DATCBB010000000
VERSION     DATCBB010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478823"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(501..866)
                     /locus_tag="VII37_07915"
     CDS             complement(501..866)
                     /locus_tag="VII37_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069699.1"
                     /translation="MGISMLRIGCVLLASATLAGCAYEFSQPQPKMVWLRLDGQRGAG
                     NPVLTQQFETNLTICLGKVQQENERSQPVDAVAKACMAEKGYIQVPEDQAEAKSQELA
                     AINPQRQAPASPSRKIPGP"
     gene            complement(956..1150)
                     /locus_tag="VII37_07920"
     CDS             complement(956..1150)
                     /locus_tag="VII37_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069700.1"
                     /translation="MKSEHPSSRIEFNYPLPSMCSAPEDHRWFWTITARVPQYPHDRG
                     YAESREQAMADFKAAWERKP"
BASE COUNT          266 a          358 c          346 g          287 t
ORIGIN      
        1 ggagagttcc taacaccacc catcacagcg aacacatgat gcggccgatg cgctgctcgc
       61 cgtcgaacac gccgtaatcg tcggtcgagc attcgccgct cgggcgggat ttggaagcgc
      121 gtttcagaac gagcatgggt cagaatagcg gtgatcgcag ccgcgaccga gacaccacgc
      181 ggcgagtatt tgcacgccgt tggcgcgcat gttgccggga gtcagccggt agagcgccac
      241 caattcccgc catgtgtaca tccgggtgaa gcgaccacac atggcctatg catctgtgtt
      301 cgtttcttgc gtcggcggcg cagcaaacgg gtcacgtctg actgcttcgc tggcaccaat
      361 ctcgtctggt tcccagaggc gccgaatgat gatgtaggtg atcacaccca cgaccggggg
      421 aacgatcagc attaccaaga gctggcccat ggctgcgtgc cttccaatcg cagaacagcg
      481 taaacctagt ttgcagtccg ctaagggcca ggaattttgc gagaaggcga tgctggcgcc
      541 tgtctttggg ggttgatagc cgccagttcc tgacttttcg cttctgcttg atcttcgggc
      601 acttgaatat agcctttttc agccatgcag gctttcgcta ccgcgtcaac cggttggctt
      661 cgctcatttt cttgctgcac ctttccaagg caaatcgtga gattagtctc aaattgttgg
      721 gtgagaacag gatttccggc tccccgttgc ccgtcaaggc gaagccaaac cattttcggt
      781 tgtggttgcg agaattcata agcgcagcca gctaacgtgg cgctcgcgag taaaacacac
      841 cctatacgca acatactgat ccccatctgt cagtctgcga cagttgcgaa atcgcaatct
      901 agcccaaaat taggttaggt cacactacgc ggcgctcacc ccggcgggtt ttgcttcatg
      961 gcttgcgctc ccacgccgct ttgaaatccg ccatcgcttg ctcacggctc tccgcgtagc
     1021 ctcgatcatg cgggtactgc ggcacccgcg cggtgatcgt ccagaaccag cggtgatctt
     1081 ccggtgctga gcacatcgaa ggcaatggat agttgaactc tattctgcta gacgggtgtt
     1141 cacttttcat gggggcttac atagcaacgc aaactgcggg cttggacgaa gaaagatttt
     1201 gccgagctta aggctcattc aaaatccaga acgccggtct cgaagatttc gaaagtg
//
LOCUS       DATCBB010000711         1236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_87395, whole genome shotgun sequence.
ACCESSION   DATCBB010000711 DATCBB010000000
VERSION     DATCBB010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87395"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VII37_07925"
     CDS             complement(<1..1198)
                     /locus_tag="VII37_07925"
                     /inference="COORDINATES: protein motif:HMM:NF006775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEY5069701.1"
                     /translation="MRDSESLAREVVARCQALARYSEDVDGIRRTFLSAAMRDCHREI
                     AGWVAPLGITSRVDAVGNLRVIYPGAKSDARRLLIGSHLDTVPNAGAYDGVLGVVIAI
                     ALLSAVNGRRFPFGIEVIGFSEEEGVRFGTPFIGSRAVAGTLDEKVLLRQDAQRISVR
                     DAIADFGLNPADIPLAAVGEDAIGYLEFHIEQGPVLETAGHPLGVVEAIAGQNRLTFT
                     FTGKSNHAGTTPMNLRRDAMAGAAEWITAVECHARTTPGLVATVGAIRVKPGVGNVIA
                     GEAELSLDVRHRDDGVRKKAVGHLSQVAESIAKSRGLNLQTTVLLDQPAVSMNPFLVS
                     QIAEAMRKACCEPYRMVSGAGHDAMIMAARIPSAMIFLRTPGGISHDPAESVEAADVA
                     KAIECGL"
BASE COUNT          275 a          389 c          316 g          256 t
ORIGIN      
        1 gcaatccgca ttcgattgcc ttagccacat cagccgcttc aaccgattcc gcgggatcgt
       61 ggctgatacc gcctggcgtc cgcaaaaaga tcatagcgga aggaattctt gccgccataa
      121 tcatcgcgtc atggccagcg ccactcacca tccgataagg ctcgcaacac gcctttcgca
      181 tagcttccgc gatttgactt accagaaatg gattcattga taccgctggc tggtctagca
      241 atacagtcgt ttgcagattt aatccgcgac tcttcgcaat actttctgcc acctgagata
      301 aatggcccac ggccttcttt cgcactccgt cgtcgcggtg gcgcacgtcg agactcaatt
      361 cggcttctcc ggcaataaca tttcctacgc cgggcttgac ccggatcgcg ccgaccgtag
      421 ccaccaggcc tggggtcgtg cgcgcatggc attcgaccgc tgtaatccac tccgccgctc
      481 cggccatcgc atcacggcga agattcatcg gcgtcgttcc ggcatgattg gatttccccg
      541 tgaaagtgaa cgtgagtcga ttctgaccgg caatcgcttc gaccacgcca aggggatggc
      601 cagcagtctc cagtactggc ccttgttcaa tgtggaattc taaatagccg atggcatctt
      661 cgccgacagc agctagtgga atgtctgccg gattcaaccc gaaatctgca atcgcgtcgc
      721 gcaccgaaat gcgctgcgca tcctgacgaa ggagaacttt ttcatcgaga gtgccagcaa
      781 ctgcgcggct tccaataaat ggcgtgccga agcgcactcc ctcctcttcg gaaaatccga
      841 taacttcgat gccgaaagga aaccgccgtc cgttcactgc actgagcaac gcgatagcga
      901 taactactcc caagacgccg tcgtaagctc cggcattcgg caccgtgtcg aggtgcgagc
      961 caatgagcaa ccgccgcgca tcggattttg ctccaggata aatcacgcgt aaatttccaa
     1021 ctgcgtccac gcgtgatgtg atccccagag gggcaaccca tccggcaatt tcgcgatggc
     1081 aatcgcgcat ggccgccgac aaaaaggtgc ggcgaatgcc gtcaacgtcc tcactgtaac
     1141 gcgcgagcgc ctgacagcgc gcaacgactt cacgagctag gctttcgctg tcgcgcaaca
     1201 agttcaccct tgacgacgaa ggcgcgcgcg aatgct
//
LOCUS       DATCBB010000712         1246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_87420, whole genome shotgun sequence.
ACCESSION   DATCBB010000712 DATCBB010000000
VERSION     DATCBB010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87420"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1246)
                     /locus_tag="VII37_07930"
     CDS             complement(<1..>1246)
                     /locus_tag="VII37_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY5069702.1"
                     /translation="DAAAYQDIRRKTVRILGLQPTDILEYRVVTTTLHAPLAPDFYLF
                     HTFAKDAIVTQEKFELDLPSARAVQLKVNPAAAASYEGKTGEGSDSRIIRRWEYRWSS
                     AEGKAKSSQDVSAEPDVALTTFTSWKQLSARLAEKFAAPENIAPEVIAKSNELTKSHE
                     KPEEKIEGIYDFVSQRIVTVELPLGSNGFRPRLPAEILSSGYATTEDKMVLFSALARA
                     AQMPAIVALAGAPDAAEKMLPRPSMFTHALVWSGKDGPGFWLDPGMEVAPFRMIGATL
                     RGKPAFLLLPDTKENSSLAPWLTVPVDLPFAASQQVNVDAVLGPDGALSAKVRYSLRG
                     DNELLLRVAFHQTAKEKWRDLAQLLSISDGFRGQVTNVNASDPSNTRHPFSVDYEIAM
                     PKFIDWSKKPVRIPALLPQLGLP"
BASE COUNT          278 a          342 c          352 g          274 t
ORIGIN      
        1 gggcagacct aattggggaa gcaaagcggg aatgcgcacc ggtttcttgg accaatcaat
       61 aaacttgggc atagcaattt cataatcgac tgagaaagga tgacgggtgt tcgacggatc
      121 cgatgcattc acattcgtga cttgtccgcg aaatccgtcg gagatggaga gaagttgggc
      181 cagatcccgc catttttctt ttgcggtctg atggaaggcc acgcgcaaca acaattcatt
      241 gtcgccacgc agcgagtaac gcaccttggc gctgagtgcg ccgtcagggc cgagaaccgc
      301 gtccacattc acctgctggg acgccgcgaa cggtaaatca accggcacgg tcagccacgg
      361 cgccaacgaa gagttttctt tggtatcggg caaaagcaaa aaagccggct tgccacgcaa
      421 agtggcgccg atcatgcgaa aaggcgccac ttccatcccg gggtccagcc aaaaacccgg
      481 accatcttta cctgaccaca ccagcgcgtg agtgaacatg gaagggcgcg gaagcatttt
      541 ctctgccgcg tccggcgcgc ccgcgagagc cacaatcgcc ggcatctgtg ccgcgcgtgc
      601 tagcgccgag aacaggacca ttttgtcttc agttgtcgcg taacccgagg aaagaatctc
      661 cgccggcaga cgtgggcgaa agccatttga tcctagcggc agttctacag tgacgattct
      721 ttgcgacaca aaatcgtaaa taccttcgat tttttcttcg ggtttttcgt gcgatttggt
      781 caattcgttg gacttggcga tcacttccgg cgcaatattt tctggcgctg cgaatttttc
      841 cgccagccgc gcggaaagct gcttccagga agtgaacgta gttagcgcca cgtccggttc
      901 ggcggaaaca tcttgcgagc tctttgcttt accttccgca gaactccagc gatattccca
      961 gcggcgaatg atgcgcgagt ccgatccctc accggttttg ccttcatagc tcgctgcggc
     1021 agcgggattt actttcagtt gcacggcgcg cgccgaaggc agatccaatt caaacttttc
     1081 ctgtgtgacg atcgcgtcct tggcaaaggt atgaaatagg taaaaatccg gtgcaagcgg
     1141 ggcgtgtagc gttgtggtga cgacgcgata ttccaggatg tccgtcggct gcagcccgag
     1201 gatgcggacc gtcttgcgtc gaatatcctg ataagcagcg gcgtct
//
LOCUS       DATCBB010000713         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_412641, whole genome shotgun sequence.
ACCESSION   DATCBB010000713 DATCBB010000000
VERSION     DATCBB010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412641"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..885
                     /locus_tag="VII37_07935"
     CDS             109..885
                     /locus_tag="VII37_07935"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY5069703.1"
                     /translation="MSDQLVIAGRAFKSRLIVGTGKYRSFQEMARAHAASGADMVTVA
                     VRRVNLTDKSKESLLDYIDREKMFILPNTAACYSAEEAIRTARLGREVGLSHWVKLEV
                     IGDQQTLFPDTEQLIEATRVLVKEGFVVLPYTNDDLIVARKLVDAGAAAVMPLAAPIG
                     SGLGIQNPANLRILRERITEVPLIVDAGVGTASDAAFAMELGADGLLMNTAIAEAEDS
                     VLMAEAMRDATIAGRKAFLAGRMPKRLYASASSPLSGVVR"
     gene            926..2101
                     /locus_tag="VII37_07940"
     CDS             926..2101
                     /locus_tag="VII37_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY5069704.1"
                     /translation="MPIKRLAYIDWMRGLACVLMIQAHCYNSWLNPEARKTLLYRWSQ
                     ELSTLPAPIFLFLSGVSFALVTEGLRRKNKSRTRIFRTAVLRGAEILGLGFLLRVQEF
                     VLGYPKSPWTDLLKVDVLNILGVSIIFMALFWRLVSSSGSSEHSGHPTTLLQSGHSVV
                     TRRSVAIGLSLLIAAAIAIATPPLWTTHRPNFLPWMLESYINGVHNFGVPTPWIFSIF
                     PWCGFAFVGLASGSFLFSDFAQRKEVMALSIVGAIGALACALSLWCDYSSVKIYSVYD
                     YWHSSPNFFLMRCGILLLLTLTIFAWCRWGWATEGFSAFIQFGKTSLLVYWVHIEFVY
                     GRLSILPKGRTTIPLATAGMAVIFAAMLGLSIWRTRAKKRAAPNLQTARAQSLSEAM"
     gene            complement(2125..2463)
                     /locus_tag="VII37_07945"
     CDS             complement(2125..2463)
                     /locus_tag="VII37_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY5069705.1"
                     /translation="MKMKVEEKGSGLLRHGPALLVLFVAVLFVHDIFGAHGYLSMRRT
                     QDGIQRVKAEIQQLNKENAALEQAVKDLKTDPRAIEKIAREELILARPGEIIIKIPQS
                     QLLPQAFNRP"
     gene            complement(2478..>2690)
                     /locus_tag="VII37_07950"
     CDS             complement(2478..>2690)
                     /locus_tag="VII37_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069706.1"
                     /translation="RVTIDASELLGRKILSVDRTDGAKFVFDDGSWMLVRLSGTEPLL
                     RLYVEAASLAASTKLTNEAKNWLLHG"
BASE COUNT          566 a          646 c          785 g          693 t
ORIGIN      
        1 gagccgcgac ccttgcttca agattgacgg ctaaattggc tgatgttttt ggctttccgc
       61 gatttgctgc tagtgtccag ccagactaaa atcaaagtcg gggaacaaat gtccgatcaa
      121 ctggtcatcg cggggcgcgc gtttaagtcg cggcttattg tcggcactgg gaaataccga
      181 agctttcagg aaatggcgcg cgcacacgcc gcgtcagggg ccgacatggt tacggttgca
      241 gtgcgccgag tcaacctcac cgataaatcg aaagagtcgc tgctggatta tatagaccgt
      301 gaaaaaatgt ttattttgcc gaatactgcg gcatgttact cggccgaaga agctattcgc
      361 actgcgcgct tgggacgcga agtgggattg tctcattggg tgaaactcga agtgatcggc
      421 gaccagcaga cattgtttcc ggataccgag caattgattg aggccacgcg cgtgttggtg
      481 aaagagggat tcgtagtatt gccatatacc aacgatgatt tgattgttgc tcgaaagctc
      541 gtggatgcgg gggctgcagc ggtgatgccg ctggcagcgc caatcgggtc ggggctgggc
      601 attcagaacc cggctaattt gcgaattttg cgagaacgca tcacggaagt gccgttgatc
      661 gttgatgccg gtgtgggtac ggcgtcggac gcggcattcg caatggaact gggcgcggac
      721 ggcctgctca tgaataccgc tatcgcggaa gccgaggact cagtattgat ggcggaagcg
      781 atgcgcgatg ctaccatcgc ggggcgcaag gcgtttcttg ccggacgcat gccgaagaga
      841 ctctacgcct ccgcttccag tcccctgagc ggagttgtac gctagagtat tcccgactcc
      901 attgggtacg acgactcagc tgcccgtgcc aatcaaaaga ttagcgtata tcgactggat
      961 gcgcgggttg gcgtgcgtgc tgatgattca ggcgcactgt tataactcct ggctgaatcc
     1021 cgaagctcga aaaacgctgc tgtatcggtg gtcgcaggag ttgtcgacgc tgccggctcc
     1081 gatattttta ttcctctctg gcgtctcctt cgcgttggtc actgaaggtc tgcggcggaa
     1141 gaacaaatct cgaacgagaa tttttagaac ggcggttttg cgcggggcgg aaatccttgg
     1201 gcttggattt cttttgcgag tgcaggaatt tgtgcttggg tatcccaagt cgccgtggac
     1261 ggatttgctc aaagtggatg tgttgaacat tttgggagtg tcgatcattt tcatggcgct
     1321 gttttggcga ctggtaagca gtagcggttc gtccgagcat agcggccacc ccacgacatt
     1381 gcttcaaagc gggcactctg tggtaacccg gagaagtgtc gcgattggat tgtcgctgct
     1441 gatcgccgcc gcgatcgcga tcgctacacc tccgttatgg acgacgcatc gcccgaactt
     1501 cctgccgtgg atgctggagt cctatatcaa cggggtgcac aattttggag tgccgacgcc
     1561 ttggattttc tctatttttc cgtggtgcgg atttgcattc gtaggattgg cgtctggttc
     1621 atttctattt tcggattttg cgcaacgcaa agaggtcatg gcgctgagta tagttggggc
     1681 gattggcgcg ctggcctgtg ctttatcgct ctggtgcgat tattcgagcg tcaagattta
     1741 ctcggtctac gattactggc acagcagtcc aaattttttc ctgatgcgct gcggtatttt
     1801 gttgctgctc accctgacga tttttgcctg gtgtcgctgg ggttgggcaa cggagggatt
     1861 cagtgctttc atacaattcg gcaaaacatc gttgctggtc tactgggtgc atatcgagtt
     1921 tgtgtatggc cgattatcga ttttgcctaa gggacgaacc actattccgc tggcgacagc
     1981 gggcatggcg gtgattttcg cggcgatgtt ggggctgtca atatggcgca cacgcgcgaa
     2041 aaagcgtgcc gcgccaaatc tacagactgc gagagcgcaa agtttaagcg aggctatgta
     2101 atcgagttcg cgcgaattga caaattacgg gcgattgaac gcttggggta gcaattgcga
     2161 ttgcggaatt tttatgatga tttcgcctgg ccttgcgaga atgagttctt cgcgggcgat
     2221 tttttcgata gcgcgtggat cggtctttaa atctttaacg gcctgctcga gcgcggcatt
     2281 ttccttattc aactgctgaa tttcggcctt cactctttgg atgccatctt gagtgcggcg
     2341 catggagagg tagccatggg cgccaaagat atcgtgaaca aaaagaaccg ccacaaacaa
     2401 aacaagcagg gctggcccgt gtcgcagtaa gccgctccct ttttcttcaa ccttcatttt
     2461 catctgtatc gttttcctta tccatgcaaa agccaattct tggcttcgtt ggtcagcttc
     2521 gttgaagcag ccaaactggc ggcctcaaca tataagcgca gaagcggttc ggtgccggaa
     2581 agccgaacga gcatccagga accgtcatcg aaaacaaact ttgcgccatc agtacgatcc
     2641 acggaaagaa tttttcgtcc cagtagttct gaagcgtcaa tggtcacccg
//
LOCUS       DATCBB010000714         1828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_379233, whole genome shotgun sequence.
ACCESSION   DATCBB010000714 DATCBB010000000
VERSION     DATCBB010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379233"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1602
                     /gene="aroC"
                     /locus_tag="VII37_07955"
     CDS             430..1602
                     /gene="aroC"
                     /locus_tag="VII37_07955"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY5069707.1"
                     /translation="MLQFYTAGESHGQALLAFISGLPASLPVDVAFINHELHRRQLGY
                     GRGGRQKIEKDRADIFAGVRHGKTIGAPVALRIENRDWANWENSLPVEESAESAGKER
                     KLVAPRPGHADLAGSQKFNFHDARYILERASARETAARVAAGAFAKLLLRELGTEIAS
                     HTIRVGHFALERAATWEEIRAVSGDLESPLRCVDADLQEKMKTEVDVALKAGNTVGGI
                     FEVMAHNVPVGLGSHAQWNEKLDGRLAQAVMSIQAVKAVEIGAGVAGAGSYGSEVQDE
                     ISYDRDAKRFLRSSNRAGGIEGGITNGQDVVVRGYLKPISTLRRALGTADMVTKEPVQ
                     AAYERSDWCVVPAGGVAGEAMVALVLADAFLQKFGGDSLAEMRRNFDNYARQIDEF"
     gene            1607..>1828
                     /locus_tag="VII37_07960"
     CDS             1607..>1828
                     /locus_tag="VII37_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5069708.1"
                     /translation="MTATTETVASTVSAGPPRKIYSIIKYGDPILEKPTAAITEFGPE
                     LEELTEDMFASMYAAQGVGLAAPQIGKNLR"
BASE COUNT          395 a          391 c          647 g          395 t
ORIGIN      
        1 acggtgaacc cgaggacact ggaatcaaga gttcgaccga ggaaccggca gcggggggat
       61 tgggagtttt taagaacaat ccgccgagtc cgaccgttcc caggcgagat gcgccacgct
      121 gctcacctga gcgccaaaca acaatcaaat ccttgggcgc gagtatgcgt ggataagtgc
      181 gtaggtcttt gtgttgagta tcgatggccg gttctccgag taaatgtcgc cgttgttaag
      241 aagatagagt ttgcgacagg tatgaatgct gggccatagc gccgacgtac tcgcctgaaa
      301 ggttggagcg tcacagagat atctagtact ttagtaatga cagaaactgg acgcgagtcg
      361 gttcgacaga tacactcctt ttcttaatct gaacagataa atccaatttc tctgaggatg
      421 cctgagggaa tgctgcaatt ttacactgcg ggtgaatcgc acgggcaggc gctactggct
      481 tttatttccg ggctgccagc ttcgctgccg gtggatgtgg cgtttattaa tcacgagttg
      541 caccggcggc agcttgggta tggccgcgga gggcggcaga agattgagaa ggaccgggcg
      601 gatatttttg ccggagtacg gcatggcaag acgattggcg ctccggtggc tttgcggatt
      661 gagaatcggg actgggctaa ttgggaaaac agtttgccgg tggaagagtc ggcggaatcc
      721 gctgggaaag aacggaagct ggtcgcgccg cggcctgggc atgcggatct ggcgggatcg
      781 cagaaattta attttcatga cgcgcggtat attttggagc gggcgtcggc gcgagagacg
      841 gctgcgcgcg tggcggctgg ggcgtttgcg aagctgttgc tccgcgaact tgggacggag
      901 attgcgagtc atacgattcg cgtgggacat tttgctttgg agcgtgccgc tacgtgggag
      961 gaaattcgag cggtgagtgg agatctggag tcgccgttgc gttgcgtgga cgcggacttg
     1021 caggaaaaga tgaaaacgga agtggatgtg gcgctgaagg ctgggaatac cgtgggcggg
     1081 atttttgagg tgatggcgca caatgttccg gtgggacttg ggagccacgc gcagtggaat
     1141 gaaaaactgg atgggcgact ggcgcaagcg gtgatgagta ttcaggcggt taaggcggtg
     1201 gagattgggg cgggcgttgc gggcgccgga tcgtatggaa gtgaagtaca ggatgagatt
     1261 tcttacgatc gagatgctaa gcgatttttg cggtcgtcta atcgcgctgg gggtattgaa
     1321 ggcggaatta ccaacgggca agacgtagtg gtgcgcgggt atttgaagcc gatttccact
     1381 ttgcggcggg cgctggggac ggcggacatg gtgaccaagg agccggtgca ggcggcgtat
     1441 gagcgctcgg actggtgcgt ggtgcctgca ggcggcgtgg caggcgaggc gatggtggcc
     1501 cttgtgctgg ccgatgcttt tctgcaaaaa tttggaggag actccctggc ggagatgcgg
     1561 cggaatttcg ataactatgc ccgacaaatc gacgagttct gagcctgtga ccgcgacgac
     1621 ggagactgtg gcttcgacag tgtcagccgg accaccgcga aagatttatt caattattaa
     1681 atacggcgat ccgattctgg aaaagcccac ggctgcgatt acggagttcg gccccgagct
     1741 ggaagaatta accgaggaca tgttcgcgtc gatgtatgcg gcgcaaggcg tggggctggc
     1801 tgctccgcaa atcgggaaaa atctgcgg
//
LOCUS       DATCBB010000715         1455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_379274, whole genome shotgun sequence.
ACCESSION   DATCBB010000715 DATCBB010000000
VERSION     DATCBB010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379274"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VII37_07965"
     CDS             <1..1165
                     /locus_tag="VII37_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069709.1"
                     /translation="VYADLCEGDSCVRSALGLPGIRLPLHENSRFGERKDGIYFIRTH
                     VLHRDRALELRFGFDERPTRVAIAQMRRLLLWTLGICFVLSTLASLLLSYLLARPRLR
                     LERFDRQLRTATRLLPSAFALHNIADDPQHARDTLATASRKVQHRERLETVGAFAGGI
                     AHEFNNLLVPITLLSDFVLKKLPANDGSRSDLKAILVAARYARALVAQILAFSREKHD
                     LNVQPLDLCDVVTEALRLFTPLISNGVRLEVELSTTCPPVLADRTLALQLVMNLCRNG
                     YQSLGGASGVLKVAVQEAVDAVGSMSCDVRARHVELSIQDSGRGMDSKTLERIFEPFF
                     TTRSETDGTGLGLTVVRGIVESFGATIQVHSVRGSGSTFRVLFPVSSAMAAAS"
BASE COUNT          296 a          392 c          434 g          333 t
ORIGIN      
        1 cgtttatgcc gatctgtgcg aaggcgacag ctgcgtacgt agcgctctgg ggctccctgg
       61 aattcgctta ccgctgcatg agaactccag atttggcgag cgcaaggacg ggatttattt
      121 catccgcact cacgtattgc acagggaccg cgcgcttgag ttacgtttcg gtttcgacga
      181 acggccaact cgcgttgcga tagcacaaat gcgccggttg ctgttatgga ctttggggat
      241 ttgtttcgta ctgtcgacgc tcgcgtcgct gctgctgagt tacttgctgg cacgcccgcg
      301 actccggcta gagcgctttg accgccagct cagaacagcg acgcgccttc taccctcggc
      361 gttcgcactg cataacattg cagacgatcc gcaacatgcg cgtgacacac tcgcgactgc
      421 gtcacgcaag gtgcagcacc gtgaacgcct ggaaaccgtt ggtgcgtttg cgggcggcat
      481 agcgcatgaa ttcaataatt tgttggtgcc gatcactctg ctaagcgact tcgtacttaa
      541 gaagctgccc gcgaacgatg gatctcgttc tgacctcaag gcgatattag tggcggcgcg
      601 gtacgctcgt gccctggttg ctcagatctt ggcattcagc cgcgaaaagc acgacttaaa
      661 tgtacaaccc ttggacctgt gtgacgtcgt aaccgaggcg ctacggcttt tcacgccttt
      721 gatctcaaac ggagtgcgat tggaggttga actttcgact acatgtccgc cggtgctcgc
      781 tgatcgcacg cttgcgctgc agttggtaat gaatctgtgc agaaacggct atcagtccct
      841 cggcggggcc tcgggtgtgc tgaaggtggc cgtgcaggaa gcagtcgacg ctgttggaag
      901 tatgtcctgt gatgtgcgcg cgcgccacgt tgaactgtca atacaggact ccgggcgcgg
      961 catggatagc aaaacgctcg agaggatctt tgagccattt ttcacgacgc gatccgaaac
     1021 tgatggcacc ggtctcggct tgactgtcgt acgcggcatc gttgagagtt ttggcgcaac
     1081 gattcaagtc catagcgtgc ggggcagtgg cagcacattc agggtgctat ttccggtgag
     1141 ctctgctatg gctgctgcct cctagcagcg cgcaggagct gccgacttaa tgttagtggt
     1201 gcattagacg ctgactttga cgaccgcgcg cgtatcgcaa gcccccgcgc gacgcgcgaa
     1261 tatggtgcag gcgttgctga atgccagccg atgccccaaa cacgtgtaat ccgcatccga
     1321 ggggcagagc acagagatgg cgcgattgca ctgaagtagc ttttgggttg cggcactttc
     1381 ggcgctaaag tttgagaaaa aaatgcgccg gctactacgc gacaaaaact tagtcgcgga
     1441 tggctagcag aatta
//
LOCUS       DATCBB010000716         1151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_153654, whole genome shotgun sequence.
ACCESSION   DATCBB010000716 DATCBB010000000
VERSION     DATCBB010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153654"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VII37_07970"
     CDS             <1..689
                     /locus_tag="VII37_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HEY5069710.1"
                     /translation="LGHTLEAPVPSLFTFHIETPWLHELAGVSVESVEASVPETKLRE
                     RGALLITHWGLSGPAILRLSAWGARELHAKNYRFTIQINWLPYLNAEKLAAAFQTCRQ
                     SQPAKFIVNTPLAKLPSRLWEQLVIASGLARDTRWAALSGAGQHQFIQQLLHTEFPVT
                     GKSLNKDEFVTCGGVKLNEVNFKTMESKICPGLFFAGEVLDIDGITGGFNFQAAWTTG
                     WLAGRAMAES"
     gene            788..>1151
                     /locus_tag="VII37_07975"
     CDS             788..>1151
                     /locus_tag="VII37_07975"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY5069711.1"
                     /translation="MAAAALLAAGIFAVVHSAGRAQQTDLSNNQIKKPTNMSDFKKPS
                     AMELKQKLTAEQFAVTQQSATEPAFHNEFWNNHQPGIYVDIVSGMPLFSSLDKFDSDC
                     GWPSFTAPLNGTDVVEHTD"
BASE COUNT          263 a          321 c          337 g          230 t
ORIGIN      
        1 cgctcggcca cacgctcgaa gcgcccgtgc cttcgttgtt caccttccac atcgaaacgc
       61 cttggctcca tgaactggcg ggcgtttcag tcgaatccgt tgaagcatcc gtgcccgaaa
      121 ccaagctccg cgaacgcggc gcgctgctga tcacacattg gggtttgagc gggccggcga
      181 tcctgcggtt gtcggcttgg ggcgcgagag agctgcacgc gaaaaactac cggttcacaa
      241 ttcagataaa ctggctgccg tacttgaacg cggaaaaact cgcggcggct tttcaaacct
      301 gccgccaatc gcagccggcc aaattcatcg tcaatacgcc gctggcgaag ttgccgtcgc
      361 ggctctggga acagcttgtg atcgcgtccg gcctcgcgcg cgatacgcgt tgggcggcgc
      421 tttcgggtgc gggacagcac cagttcatcc agcaattgct gcacacagaa ttcccggtca
      481 cgggtaaaag cctgaataag gacgagttcg tgacgtgtgg cggcgtcaag ttgaacgaag
      541 tgaatttcaa gacgatggag agtaaaattt gtccgggact ttttttcgcc ggcgaagtgc
      601 tggacattga tggcatcacg ggtggattca atttccaagc cgcctggacg accggctggc
      661 tggcgggtcg cgcaatggca gaaagctgat tagtgttgta aggtttcggt gatttgcgcg
      721 actcatgttt gaacatgagt ggccatgaaa tcatcaaacc agtatgcaac cgataaaaat
      781 tgcgggcatg gcggccgcgg cgttgctggc ggcgggcatt tttgccgtgg ttcacagcgc
      841 gggccgcgct caacaaacag atctttcgaa caaccaaatc aagaaaccaa cgaacatgag
      901 cgatttcaag aaacccagcg caatggaact gaagcagaaa ctgacggccg aacaatttgc
      961 cgtcacccag cagagcgcca ccgaaccggc gttccacaac gagttttgga acaaccacca
     1021 gcccggcatc tatgtggaca ttgtgtcggg catgccgctg ttcagttcgc tggacaagtt
     1081 tgattccgac tgcggctggc cgagcttcac cgcgccactg aacggcacgg acgtggtcga
     1141 gcacaccgac a
//
LOCUS       DATCBB010000717         1281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_282232, whole genome shotgun sequence.
ACCESSION   DATCBB010000717 DATCBB010000000
VERSION     DATCBB010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282232"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..892)
                     /locus_tag="VII37_07980"
     CDS             complement(41..892)
                     /locus_tag="VII37_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY5069712.1"
                     /translation="MPATLKMRHDAHYVESLTSYSGAAVGRMIPVDQIRPNPDQPRKA
                     LGDLRELTDSIREKGVLEPLLVRFIAREDCFYIISGERRYHASRAAGLREVPCIEKTA
                     DDAETLEIGLIENLQRKDLTAFEEAEGLDRLGTQFEYTHDDIARKIGRARTSVTETLS
                     LLHIPEPLRKKCAEQGINTKSLLLQIARQPTEKKMMETVQRIMQGGLTRDEARRERQE
                     HVAGPQRPQPFLFQFEPEDEEFKFRIQFKKSHVTRDELISTLREILSQLEGDSSSSSS
                     KADSTAA"
     gene            complement(864..>1281)
                     /locus_tag="VII37_07985"
     CDS             complement(864..>1281)
                     /locus_tag="VII37_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY5069713.1"
                     /translation="SLGILTVNALVASTHLLVPIQAAYFAIEGTDDLLETYERIRARP
                     NPGLKMLGVVITLFDKRTNISRDTHEQIRTVFGEVLFKTKISKNVRLEESPAYKETIL
                     TFAPKSPGAVEYKKLAGEVIQRVEENRLASHAEDAA"
BASE COUNT          274 a          398 c          298 g          311 t
ORIGIN      
        1 gtctgagact acgtcaaact gccgtcatag ctgctgacaa ctacgccgcg gtcgaatcag
       61 ccttcgagga cgagctcgac gaatcgcctt ccaactgcga gaggatctcc cgcagcgtgc
      121 tgatcaattc gtcgcgcgta acgtggctct tcttgaattg aatcctgaat ttgaattctt
      181 cgtcttccgg ctcaaactga aacaggaacg gctgcggcct ctgcggcccc gcaacatgct
      241 cctggcgttc cctgcgcgcc tcatcccgcg tcaagccgcc ctgcataatg cgttgaaccg
      301 tttccatcat cttcttttca gtaggctgcc gcgcaatttg cagcaacagc gacttggtgt
      361 taattccttg ctccgcgcac ttcttgcgca gcggttccgg aatgtgcagc agcgagagtg
      421 tctcggtaac agaagtccgc gcccgtccga ttttccgtgc aatgtcgtca tgcgtatatt
      481 caaactgcgt gcccagtcgg tcgagtccct cagcttcctc aaaagccgtc aagtccttgc
      541 gctgcaaatt ctcgatcagc ccgatttcta gcgtctcagc atcatccgcg gttttttcta
      601 tgcacggcac ttcacgcaat cccgctgcgc gcgaagcgtg atacctgcgc tccccagaaa
      661 taatataaaa gcaatcctcg cgcgcgataa accgcactag cagcggctcc agcacgccct
      721 tctcgcgaat cgagtctgtc agttcgcgca aatccccaag agccttgcgc ggctggtccg
      781 ggttcggacg aatctgatcc accggaatca ttcttcctac tgccgcgccg ctgtagctcg
      841 ttaacgattc gacgtagtgc gcgtcatgcc gcatcttcag cgtggctggc aagccgattc
      901 tcttcaacac gttgaataac ctccccggcc aatttcttat actccaccgc tcccggcgat
      961 ttgggagcga aagtcaaaat ggtttccttg tatgcgggac tctcttcgag ccttacattt
     1021 ttactgatct tcgtcttaaa caatacttcg ccaaacaccg tgcgaatttg ttcgtgtgta
     1081 tcgcgcgaaa tatttgtgcg cttatcaaac agagtaatca ccacgcccaa cattttcaaa
     1141 ccaggattgg gccgcgcgcg aattcgctcg tacgtttcca gcaaatcatc cgtgccttcg
     1201 atcgcaaaat acgcggcctg aatcggcacc agcaaatgag tggaggccac cagcgcattc
     1261 acggtcaaaa ttcccagcga a
//
LOCUS       DATCBB010000718         1093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_249356, whole genome shotgun sequence.
ACCESSION   DATCBB010000718 DATCBB010000000
VERSION     DATCBB010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249356"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VII37_07990"
     CDS             <1..213
                     /locus_tag="VII37_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069714.1"
                     /translation="ITSQALVDRISGAGNREVTYAGSFSDAAAAVADRAQPGDMVLTL
                     GAGSVFQLGGMILEKLARTATPNISR"
     gene            546..>1093
                     /locus_tag="VII37_07995"
     CDS             546..>1093
                     /locus_tag="VII37_07995"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY5069715.1"
                     /translation="MAAIAACAILSAAAFNLYGYGKHSWRFRIDSSDQIEVAGAEHVA
                     HAQIMEVMGGDIGRNIFFIPLAQRQQQLEQIPWVESASVMRFVPNRLRIEIHERTPVA
                     FARIGSHISLIDAGGTLMELAPGVKRKYSFAVIAGMNSGEPLSTRAARMKIYNALVRS
                     LDSTGAHYSRELSEVDLSDPEDV"
BASE COUNT          227 a          322 c          295 g          249 t
ORIGIN      
        1 atcacctcgc aggcgcttgt cgatcgcatt agcggagcag ggaatcgcga agtcacctac
       61 gccggatctt tttccgatgc cgctgctgca gtcgcggatc gcgcacagcc aggtgacatg
      121 gtgttgactc tcggcgcggg aagcgttttc cagctcggtg gaatgattct cgagaagctc
      181 gcacgcactg ctactcccaa tatttcccgg taaagccccg gcgaacctgc gactgtggaa
      241 atcattgttc aattggaccc aacttcgcac tgtctcaatt cgcgaaaaca cgttatagtg
      301 tttttgctca gcggttttct gtcgacaatt gactcgaaag agtggcccgg aaatcaagtt
      361 ccaccgcggt ccaggaagag ttgtacccac ttccgaacga ggctcccgag cgggaggcct
      421 tcgacgatgc acggctcgtc gatttggaag cagagcagga atctcctttc ctgcgcgggc
      481 aaaagcgagt ctcggtccgc cgcggatccc ttcccaaaaa aactgccaat cgattgctct
      541 ggataatggc cgcgattgcc gcttgtgcaa ttctgagcgc tgctgccttc aatctctacg
      601 gctatggcaa gcactcctgg cgttttcgca tcgattccag cgaccagatc gaagtggccg
      661 gagccgaaca cgttgcccac gcccagatca tggaagtcat gggcggcgat attggccgca
      721 acattttctt tatccctctg gcccagcgcc agcagcagct agagcagata ccgtgggtgg
      781 aatcggcgag cgtgatgcgg tttgttccca atcgcctgcg cattgaaatc cacgaacgta
      841 cgccggttgc ttttgcccga atcggttcgc acatatcgct gatcgatgcc ggcggtacgt
      901 tgatggaact ggcccccggc gtaaaacgca agtattcatt tgccgttatt gcaggcatga
      961 attccggcga gccgctttcc actcgcgccg cgcgcatgaa gatctacaac gctcttgtcc
     1021 gctccctcga ttcaaccggt gctcattatt cgcgcgagct cagcgaagtc gatcttagcg
     1081 atcccgaaga cgt
//
LOCUS       DATCBB010000719         3988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_184491, whole genome shotgun sequence.
ACCESSION   DATCBB010000719 DATCBB010000000
VERSION     DATCBB010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184491"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VII37_08000"
     CDS             <1..1050
                     /locus_tag="VII37_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069716.1"
                     /translation="MSISILPPDVNESDLYFTPVNEAIRFGMAAIKNVGENTAKAIRE
                     SRIAQGEFRSLYDFCERIEPRFLNKRVFESLIKSGAMDSLGPREGMLASVDEAVNALQ
                     RASRSRESGQHGLFGTAAAPAPILFELREAAPWSEDERLTSEYAMLGFYVSGHPLARY
                     ASRLQDMKALTLDQVEGQRNNKDITVAALIVGIRPMRTRKGARWGIFTLQDMTGVQEM
                     LVFPETFTRLESMLKPGVPLLLKARVQVEEAGTRLSLQEARRLEDIAERAAAPQEFRV
                     RMDMQAINPDCLDRLEMLFGNFPGTCVVVFELRSADGSVALLQAQQRVRSNPELVEAV
                     RHICGDRAVEMAAAV"
     gene            1047..2066
                     /locus_tag="VII37_08005"
     CDS             1047..2066
                     /locus_tag="VII37_08005"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY5069717.1"
                     /translation="MSAKDKEKEKEKEKDKDKNKAKSNHTARQKEIERLEKDVEDLRN
                     LAGDEASDAEFERVSKQVAELRREFYAHLGPWQRAQIARHQNRPYTLDIIGMLFTDFI
                     ELHGDRNFGDDKALVTGFAKFHGRPVAVIGHQKGRDTKQRVQRNFGQPKPEGYRKALR
                     VMQLAAKFCRPIFTFIDTPGAYPGIDAEERGQAEAIARNLREMSRLPVPIIVTVTGEG
                     GSGGALAIAVGDRVNIFENSFYSVISPEGCAAIMWRDSSKAEIAASALKITASDLKEF
                     GIIDEIIPEPEGGAHTDPEAASRLLDAVLDRQLLMLSQQPQKELLETRYEKFRKMGQF
                     FDLGG"
     gene            2229..2549
                     /locus_tag="VII37_08010"
     CDS             2229..2549
                     /locus_tag="VII37_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069718.1"
                     /translation="MSEPFRPYEKLVEITLLGRKFQVPERNSLLRCFQFISPETIPYG
                     RFCWNQECQYCRVSCQLPDDDESREMLSCKFIVMAGMEITEISQELKWCLRAKLPSDT
                     PVSS"
     gene            3298..3735
                     /locus_tag="VII37_08015"
     CDS             3298..3735
                     /locus_tag="VII37_08015"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY5069719.1"
                     /translation="MQKRAKFGVGIGVIVISLGFLAWMGYGESKTYYHTIAELQTLQG
                     GALSHRMRVGGTVEPGSIKRLSGRVDFVLQGEGKSLPVSYVGSEPLPDTFVDKSQALV
                     EGRPAADGRFVAEHVQAKCASKYEAAPGGTPAPAADGARTSTM"
     gene            3758..>3988
                     /locus_tag="VII37_08020"
     CDS             3758..>3988
                     /locus_tag="VII37_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069720.1"
                     /translation="MGGSVDVFGSFALLLAFICAAYAFVGGIAAIRTRHPLLVKSTRQ
                     AGLATCGLIFLATFSLEYLFFSDNFSIAYVVAH"
BASE COUNT          869 a         1094 c         1140 g          885 t
ORIGIN      
        1 atgagcattt cgattcttcc gccggacgtg aatgagagtg atctgtactt cacgcccgtc
       61 aatgaagcga ttcgcttcgg tatggcggct atcaagaatg tgggagaaaa caccgccaag
      121 gccattcgcg aatcgcgcat cgcccaaggc gaattccgct cgctttacga tttttgtgag
      181 cgtatcgaac cgcgcttcct caacaagcgt gtctttgaaa gcctgatcaa gtccggagcg
      241 atggattcgt tgggcccgcg cgaaggcatg ctggcatccg tggatgaggc tgtgaatgcg
      301 ttgcaacgtg cgtcacgttc acgcgagtcg gggcagcacg gactattcgg aacagcagct
      361 gcacccgcgc caattttgtt cgaactgcgc gaggccgcgc cgtggagtga agacgagcgg
      421 ctgaccagcg aatacgccat gctcggtttt tacgtgtcgg gacacccact ggctcgctat
      481 gcatcgcgct tgcaggatat gaaagcgctg actctcgacc aggtcgaagg ccagcgcaac
      541 aataaagaca ttaccgttgc ggcgctgatt gtcggcatcc gcccgatgcg caccaggaaa
      601 ggcgcgcgct ggggcatttt cactttgcaa gatatgaccg gagtgcagga gatgctggtg
      661 tttcctgaaa catttacccg ccttgaatcc atgctgaaac caggtgtccc gctgttactc
      721 aaagcgcgcg tacaagtcga agaagcggga acacgtttgt cacttcaaga ggcccgccgc
      781 ctggaagaca ttgcggaacg tgcagctgcg ccgcaggaat tccgcgtacg catggatatg
      841 caagctatca acccggactg cctcgatcgc ctggaaatgt tgttcgggaa ttttccgggg
      901 acgtgcgtcg tcgtgtttga gttgcgttcg gctgatggct cggttgcgct gttacaggcc
      961 cagcagcggg tgcgttccaa ccccgaactt gtcgaggcgg tgcgccacat ttgtggcgat
     1021 cgcgccgtgg aaatggcggc cgctgtatga gcgccaagga caaagagaaa gaaaaagaga
     1081 aggaaaagga caaggacaag aataaggcca agtccaatca caccgcccgc caaaaagaaa
     1141 ttgaacgcct cgaaaaagat gtagaagatc tgcgcaacct cgccggcgat gaagcttccg
     1201 atgcggaatt cgaacgcgtc agcaaacagg tcgccgagct tcgccgggaa ttctatgccc
     1261 acctaggccc ctggcagcgt gcgcagattg cgcgccacca aaaccggcct tacactctcg
     1321 acatcatcgg catgcttttt accgatttca tcgagctgca cggcgatcgc aattttggcg
     1381 acgataaagc gctggttacc ggttttgcca aatttcatgg ccgcccggtg gcggtcatcg
     1441 gccaccagaa aggccgcgac actaagcagc gtgtgcaaag aaatttcggc cagcccaaac
     1501 cggaaggtta tcgcaaggcg ctgcgcgtga tgcagctggc tgcaaaattc tgccggccga
     1561 ttttcacttt tatcgacacg ccaggcgcgt acccgggcat tgatgccgaa gagcgcgggc
     1621 aggccgaagc catcgcgcgg aaccttcgcg aaatgtcgcg cttgccagta cccattattg
     1681 tcacggttac aggagaaggc ggatccggtg gcgccttggc gattgccgtg ggagaccgcg
     1741 taaatatatt cgagaacagt ttttattccg tgatatcccc cgaaggctgc gcggctatta
     1801 tgtggcgtga ttccagcaaa gcggagattg cggcctccgc gctgaaaatc accgcatcgg
     1861 atttgaagga atttggaatc attgacgaaa tcattccgga gcccgaaggc ggcgcgcaca
     1921 ccgaccctga agcagccagc cgtctgctcg atgccgtgct cgaccggcag ttgctgatgc
     1981 tttcgcagca gccccagaaa gaactgctcg agacacgcta cgaaaagttc cgcaagatgg
     2041 ggcaattctt cgacttaggt ggctaacgcc gctgagctaa cgtggctggt aaagaacgcc
     2101 tccgctgacc cttggcacat tcgcaactct cctcaatacc tctggttgtt gccatctcgt
     2161 tgggggacta atctggagac gtgttcggat cgcctagagt ctgggcccgg gcgcggagga
     2221 atctgcagat gtccgagccg ttccgccctt acgaaaaact cgtggagatc acgctactgg
     2281 gaagaaaatt ccaggtccca gaacggaact cgttgctgcg ttgtttccaa ttcatcagtc
     2341 ccgaaacgat tccatatgga cgcttctgct ggaatcagga gtgccaatat tgccgtgttt
     2401 cctgccaact cccggatgac gatgagtccc gtgaaatgtt gagttgcaaa tttatcgtca
     2461 tggccggaat ggaaatcacg gagattagcc aggaactcaa gtggtgcctg cgcgccaagc
     2521 tgccttcgga tacgcccgtt tcgtcctaat tccgcgttac gtttgctctc gctgttcctc
     2581 tgcattacat tgctaagcat cgatgaacgc caatacttca gaaatcttgt gcgacggctg
     2641 cggccagcct gcttctccgg aacacctttc gcgacgtttg caacgattgg aatgggctac
     2701 tcgttaccgg ccaattcatc tgcatacgct gttgctcggg gcgatttcac cacaggcgtc
     2761 agccgagttc ctgtattcgc ctgaggatcc acacttcgga gaagcggcga atcttattgc
     2821 cgccgctgga atctcagccg gagataaatc cgcggacgtg attcaatcag aggttcagcg
     2881 gcgtgggatt tacatgacgc acgcgctcga atgcccgttg gaggtcccat ccaggggaga
     2941 tagctttttg aacagcgtat tgattgcccg cctacctatg ctcttcacga ggattcgccg
     3001 atcactaaaa cctaaacgaa tcgcattcgt ttccgggagc ttgacgcctc tcattgagcg
     3061 gttcgcctcc gcacagctaa attgtgagct ggccctcgat gatggtcgag cattcgcgtt
     3121 agataattca aatcccgccg aggtgaaggc ggcaattaca cgctttcgcg acgtcctagc
     3181 cgtgcccgca agtcgctagt cgggcgaggg ttactcccaa ggtgttcgcg cggtgcccgt
     3241 ttttgagctg tgatagaatc ggtctaactc ggatcggggc tattgcgagg tgcagctgtg
     3301 cagaaacgcg cgaaattcgg cgttggaatc ggtgtcattg ttatctccct cggttttctc
     3361 gcctggatgg gctacggcga aagcaaaact tactatcaca ccatcgccga actgcaaacc
     3421 ctacagggcg gagcattaag ccatcgcatg cgcgtgggag gcacagttga gcccggtagt
     3481 atcaaacggc tctccggccg cgtggatttt gtgttgcaag gggaagggaa gtcgctgcct
     3541 gtcagttacg tgggtagcga gccattgcct gataccttcg tcgataaatc gcaagcgttg
     3601 gtagaaggcc gtcccgctgc ggacggccgt tttgtagcgg aacacgtgca agcaaagtgc
     3661 gcgtcaaaat acgaagctgc tccaggtggc acaccagctc ctgccgctga cggagcgcgc
     3721 accagcacta tgtaacactc ctctgttcgg ggaggaagtg gggggctccg tggatgtatt
     3781 cggttctttt gcgctgctgt tggcgtttat ttgcgcggcg tacgcgtttg tcggcggaat
     3841 tgccgccatc cgcacgcgcc atccgttgct ggtcaaaagc actcgtcagg cggggcttgc
     3901 gacatgcggt ctgatattcc ttgctacctt cagtctcgaa tacctgttct tcagcgataa
     3961 tttttccatt gcctacgttg tagctcac
//
LOCUS       DATCBB010000720         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_444044, whole genome shotgun sequence.
ACCESSION   DATCBB010000720 DATCBB010000000
VERSION     DATCBB010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444044"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VII37_08025"
     CDS             complement(<1..442)
                     /locus_tag="VII37_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY5069721.1"
                     /translation="MPAVSPHHVAVFRVLWRTVNALLIASLLFVGYSATWEYSVRRYL
                     KGFSDAIVPSTATPEEKIDSILTWIRSGAPRPLAPNLDELSIRDPETTLNYSQLLAIC
                     GTATNAFLNLSRSAGLDARRLLLLTPERTTKHVVAEVLVDGRWII"
     gene            complement(451..1692)
                     /locus_tag="VII37_08030"
     CDS             complement(451..1692)
                     /locus_tag="VII37_08030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5069722.1"
                     /translation="MRILNVTETYDPFLEFGGPPVKVRALAHGLARRGHAVNVLTADW
                     GLEKRLAHHSESDLQTLGWDRSPFGWRHAQDGVQSIYLPSWFRYRALSWNPAVKRYCR
                     ARLQNFDVVHIFGIYDLLGPPVAAAALARNMPYVVEPIGMFVPIVRNVWLKRMYHYFV
                     GRRLFAGASAVIATSDLEVQELAAAGVAPEKIVLRRNGVESPASWPPRGKFRAAHDIP
                     EQAKLILFLGRLSLKKSPELLLHAFGNLPERIGEHALMLAFAGPDENSMQQHLVATAS
                     QLGMSSRVKFSGPLFGEDKWAAYRDADVFVLPSLNENFGNTAAEAVAASTPVVVTEQC
                     GIAPLVKDVAGLVVAHDETSVANALQRLLGDSQLHAKFVSGCAELTSRLSWQEPVAQM
                     ESLYATLTTPAVSGSQPRSME"
     gene            complement(1680..>2302)
                     /locus_tag="VII37_08035"
     CDS             complement(1680..>2302)
                     /locus_tag="VII37_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5069723.1"
                     /translation="DPEFESVLPAASKAPPPPGFPPGRVILTVGRWLATERYKGMDTL
                     INSLPRLLPRWPELQLAMVGEGDDRDWLENLADKNGVLSHVHFLSGLSYSQLASCYSA
                     CEIFALPSQAEGFGLVYLEAMACCKPVIGGAHGGAPEVIIDGVTGYLVQHGDAPQLSI
                     AIETLLSDPALAREMGARGCQRVEHEFRFSVFAKALKKILREQCES"
BASE COUNT          523 a          584 c          759 g          436 t
ORIGIN      
        1 cgatgatcca gcggccatcc acgagtacct cggcgacgac gtgcttggtg gtgcgttcgg
       61 gagtgagcag caggagacgg cgagcgtcga ggcctgcgct gcgggagaga ttcaagaacg
      121 cattggtggc ggtgccgcaa atggcgagga gttggctgta gttcagggtg gtttcgggat
      181 cgcggatgga gagttcgtca aggttaggag ccagagggcg aggggcgcca ctgcgaatcc
      241 aagtcaggat ggagtcgatt ttttcttcgg gggtggctgt gctgggaacg atggcgtctg
      301 agaagccctt gaggtagcgg cgcacggaat attcccaagt ggcggagtaa ccgacgaata
      361 gcaatgaagc gatgagcaga gcattgaccg tgcgccagag tacgcgaaag acggctacgt
      421 gatgggggct cacagcgggc accgtattac tcactccatg gaccggggtt gagagccgga
      481 aactgccggc gtcgtcagtg tggcatacaa ggattccatt tgcgccaccg gttcctgcca
      541 gctcaagcga gaggtgagct cggcgcaacc ggagacaaat ttggcgtgaa gctgcgaatc
      601 gccaagcaaa cgctgcaagg cgtttgcaac ggatgtttca tcgtgggcga cgaccaagcc
      661 ggcgacgtct tttaccagcg gagcaatgcc acattgttcg gtaactacta ccggggtgga
      721 cgcggcgacg gcttcggcgg cggtgttgcc aaagttttcg ttcagcgatg gcagcacaaa
      781 aacgtcggcg tcacgatagg ccgcccattt atcttcgccg aatagagggc ctgaaaattt
      841 tacgcgcgag gacatgccga gttgggaagc ggtagcgacg agatgctgct gcatggaatt
      901 ttcatccggt ccggcgaacg ccagcatcaa ggcatgttcc ccaattcgtt ctggaaggtt
      961 cccgaaagcg tgaagcaata attccgggct ttttttcagc gacaaacggc ccagaaataa
     1021 tatcagtttg gcctgttcag gaatgtcgtg agcagcgcgg aacttgccgc gcggcggcca
     1081 ggaagcaggc gactctacac cattccgacg aaggacgatt ttctcaggcg cgacgccagc
     1141 ggcagccaac tcctgaactt ccagatcaga ggtggcgata accgcgctgg cgccagcaaa
     1201 taatcgccgg ccaacaaaat aatgatacat ccgtttcagc cagacgtttc ggacaattgg
     1261 gacaaacatg ccgatgggct cgacgacgta gggcatgttt cgtgctagtg ccgcggcggc
     1321 gacaggtggt cccagaagat cgtaaatacc gaaaatgtgg accacatcaa aattttgcag
     1381 acgagcgcgg cagtagcgct taaccgcggg attccaactt agcgcgcgat agcgaaacca
     1441 gctgggcaga tagatggatt ggacgccgtc ctgggcgtgg cgccagccaa agggcgaacg
     1501 gtcccaacca agggtttgaa gatcggattc ggaatggtgg gccaggcgct tttcaaggcc
     1561 ccagtccgcg gtcagcacgt ttacagcgtg gccgcggcgc gccagaccgt gggccaaggc
     1621 gcgcactttt actggagggc cgccgaattc cagaaaagga tcgtaggttt cggtgacgtt
     1681 caggattcgc attgttcgcg aagaattttt tttagcgctt tagcgaaaac gctgaagcga
     1741 aattcgtgct ctacacgttg gcagccgcgc gcgcccattt cgcgtgcgag agccgggtcg
     1801 gagagcaacg tttcgatggc gatggaaagc tgtggtgcgt cgccgtgctg tacgaggtat
     1861 ccggttactc catcgatgat cacttcgggc gcgccaccat gagccccacc gatgaccggt
     1921 ttgcagcagg ccatggcttc gaggtagacg aggccaaaac cttcggcttg gctcggcagc
     1981 gcaaaaattt cgcaagcaga gtagcacgag gccagctgcg aataggaaag tccgctgagg
     2041 aagtggacgt gagagaggac accatttttg tccgcgagat tctcgagcca gtcgcgatca
     2101 tcgccttcgc cgaccatggc cagctggagt tcaggccagc gcgggagcaa gcgcggaagc
     2161 gaattgatga gggtgtccat gcctttatag cgttcggtgg cgagccaacg gccgacggtg
     2221 agaattacgc ggccgggggg aaaaccgggg ggaggaggcg ctttggatgc agcgggaaga
     2281 acggattcga attctggatc ga
//
LOCUS       DATCBB010000721         1918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_153737, whole genome shotgun sequence.
ACCESSION   DATCBB010000721 DATCBB010000000
VERSION     DATCBB010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153737"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1768)
                     /locus_tag="VII37_08040"
     CDS             complement(311..1768)
                     /locus_tag="VII37_08040"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006629.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY5069724.1"
                     /translation="MQSLRLNPILNTDSYKLTHWWQYPPDTRHIYSYVESRGGMFDET
                     MLAMLQYIIKSNFAGNVFTLEDVEETRRIAHAHFQGHPKSFNYEGFKSLYAKHGGRLP
                     LRIRAVKEGTVVKSHNALITIENTDPEFYWLTNWAETVLLQVWYPITVATLSRAIKQV
                     IGKALVRTGDPALLPFKLHDFGFRGVSSKESAAIGGAAHLLNFFGTDTLAAIQLLNQF
                     YSADLARDKPLDCAGYSIPASEHSTITAWGEEHELEAYANILKNCPEGLVACVSDSYD
                     IYNAVRNLWGGALREKILQRNGTLIIRPDSGDAVTVLEELFKIVSEVFGYEVNRKGWK
                     TTVPCVRFIQGDGVNFYTIQNITAQLTRRGWSQDIWSYGMGGALLQQVNRDTLKFALK
                     CSAIDRDGKWQNVYKNPITDPSKASKGGRFNLIQNGKEFATVEVVDGEDLPPSPRSGS
                     NALETVLEDGVILRDQSLAEVRAIAGTYDTYNEAF"
BASE COUNT          442 a          491 c          566 g          419 t
ORIGIN      
        1 ggtcaccggg cgaggatgct ccaggataac cagcaccgca ccaactactg gcagcgccga
       61 cgccagaagc acagtgctca acgtcgtcac tcgcatacgt ttcatcattc acctgaatta
      121 aattctggct gggggcgccg attggctggc gcttagacga ggagcatcta aaacaccaaa
      181 agtggactta tgtaactgta actaaataaa atagttagcg agatgacccg aaagacacgt
      241 atgccgggga acgaaaatac cggaaaaatt tacaggctgt cgcggcggat gcgggagacg
      301 aacggacgaa tcagaatgct tcattatagg tatcgtaagt cccggcgata gcacgcactt
      361 cggccagcga ttgatcccgc aggatcacgc catcttccag aacggtttca agggcgttcg
      421 aaccagatct cgggctgggc ggaagatcct cgccgtcgac aacttcgacg gtggcaaatt
      481 ccttgccgtt ttggattaga ttaaatcggc cgcccttgct ggccttgctg gggtcagtga
      541 tgggattctt gtaaacgttt tgccatttcc cgtcgcggtc gatggcggaa catttcagcg
      601 caaacttcaa cgtgtcgcga ttcacttgct ggagcagtgc gccgcccatg ccgtaactcc
      661 agatgtcctg ggaccagccg cggcgcgtca gttgcgcggt aatattttgt atggtgtaga
      721 aatttacgcc gtctccctgg atgaatcgca cacacggcac ggtggttttc cagcccttgc
      781 ggttcacttc gtagccgaac acttcactga caattttgaa cagctcttcg agcaccgtta
      841 cggcatcgcc ggagtcgggg cgaatgatca gcgtgccatt acgttgcagg attttttcgc
      901 gcagcgcgcc gccccataga ttgcgcaccg cgttgtagat gtcgtacgaa tcgctgacgc
      961 aggcgaccag gccttcggga caatttttca ggatattggc gtaggcctcc agctcgtgct
     1021 cttcgcccca ggcggtaata gtggaatgtt ccgaggccgg gatgctgtag cccgcgcagt
     1081 ccagcggttt gtcgcgggca agatcggcgg aataaaactg gttgagcagt tgaatggcgg
     1141 ccagagtgtc ggtgccgaaa aaattcagca gatgcgcggc gccgccgata gccgcggatt
     1201 ccttggacga gacgccgcga aatccgaagt cgtgcaattt aaagggaagc agcgcaggat
     1261 cgccggtacg caccagcgcc tttccgatca cctgcttgat ggcgcgcgaa agcgtggcca
     1321 cggtgatggg ataccagacc tgcagcagga cagtttcggc ccaattggtc agccagtaaa
     1381 actctgggtc ggtattttcg atggtgatca gcgcgttgtg actcttgacc accgtgcctt
     1441 ctttaaccgc gcgaatacgc agcggcaacc gcccgccatg cttggcgtat aacgatttga
     1501 agccctcgta gttgaaagac tttggatgtc cctggaaatg agcgtgggcg atgcgccggg
     1561 tttcttccac gtcttccagg gtaaaaacgt tccccgcgaa attggatttg atgatgtatt
     1621 gcagcatggc cagcatggtt tcatcgaaca tgccgccgcg cgactccaca taggaataga
     1681 tgtggcgggt atcgggtgga tattgccacc agtgagtgag tttgtaggaa tccgtgttga
     1741 ggatggggtt caggcgcagc gactgcatcg aaactcctcg ccggaagtaa atagaaagcg
     1801 gaggcaagcc aagctaagct ttgcttgagg ggcaggcacg caatgttcgg acgaaggatt
     1861 gattggcact gatatgagaa tagcaaagaa catgatgaaa acggctgtgc tggcaggg
//
LOCUS       DATCBB010000722         1278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_347406, whole genome shotgun sequence.
ACCESSION   DATCBB010000722 DATCBB010000000
VERSION     DATCBB010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347406"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VII37_08045"
     CDS             complement(<1..410)
                     /locus_tag="VII37_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5069725.1"
                     /translation="MIAQIKNQKSKVFLMPQKLQIDKWLFSATAGLALFGVVMVYSAS
                     AVIALQENHSQFHYVIKQGVWTMLGFVVMFAAMRLDYQRLNRRWIVYGVLLVTVFLLL
                     AVFAFSPVNGARRWIKFGGFSAQPSEIAKLALAL"
     gene            complement(422..>1278)
                     /gene="murD"
                     /locus_tag="VII37_08050"
     CDS             complement(422..>1278)
                     /gene="murD"
                     /locus_tag="VII37_08050"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY5069726.1"
                     /translation="ETIVDFHPAVAAVLNVTPNHMDRYDSLVGYAAAKHRVFANQTAS
                     DVAILNADDEIVSSWAPGLRAHVVQFSTKRELEEGLFLRGRDLVSRTKDGERVLLTRD
                     EMLLRGTHNVENVLAAMAAGLACGVGPESLRETVGRFRPVEHRLEEVAEINGVRFFND
                     SKATSVDATMKALEAFANEPGKVVLILGGRGKQAPYSPLAELIRKRVRKMILIGEDAP
                     AIERELKSAAPFEPAADMHDAVVRAFAATQAGDVVLLAPACASFDMFESFEHRGRVFK
                     EEVKKLRF"
BASE COUNT          301 a          414 c          305 g          258 t
ORIGIN      
        1 aacagcgcca gcgccagctt cgcaatctct gacggctgcg ccgagaagcc gccaaacttg
       61 atccaacggc gcgcgccatt taccggcgaa aaagcaaaga ctgccagcag cagaaagaca
      121 gttaccagca acaccccgta cacgatccag cgtcgattca agcgctggta atccaagcgc
      181 atcgccgcga acatgacgac gaacccgagc atcgtccaga cgccttgctt aatgacgtaa
      241 tggaactggc tgtgattttc ctgcaacgca atgacggccg atgcgctgta aaccatgact
      301 acgccgaaca gcgccagccc cgccgtcgcc gagaataacc atttatcgat ttgtaacttt
      361 tgcggcatca gaaacacttt cgatttctga ttcttgattt gcgcgatcac aaatccaaaa
      421 tctaaaatcg caacttcttg acctcttctt taaatactct tccccggtgc tcgaaactct
      481 cgaacatatc aaagctcgca cacgccgggg ccagcagcac tacatcgccg gcctgggtcg
      541 ctgcaaatgc gcgcacgacc gcatcgtgca tgtcggcagc cggctcgaaa ggcgcggctg
      601 attttagttc gcgttcgatt gccggcgcgt cttcgccaat cagaatcatt ttccggaccc
      661 gcttgcgaat caactcagcc aaaggcgagt agggcgcctg ctttccccga ccgccaagaa
      721 ttagaactac ttttcccggt tcgttggcga atgcttccag ggccttcatc gtggcgtcca
      781 cgctggtcgc ttttgaatca ttgaagaaac gcacgccatt gatctcggcg acttcttcca
      841 gccgatgctc aaccgggcga aagcgtccca cggtttcgcg caacgattcc gggcccacgc
      901 cacacgccaa tcctgcggcc attgccgcca gaacattctc gacattgtgc gtaccgcgca
      961 acagcatttc atcccgcgtc aacaaaacgc gctcaccatc tttggtgcgc gaaaccagat
     1021 cgcgtccgcg cagaaacaaa ccttcctcca actcgcgttt ggtgctgaac tggacgacgt
     1081 gcgcccgcag gcctggcgcc cacgacgaca cgatttcatc gtcagcgttc aaaatcgcca
     1141 cgtcgctcgc ggtctggttc gcaaacacgc ggtgcttcgc ggccgcatag ccaactaaag
     1201 aatcgtaacg atccatgtga ttcggcgtaa cgttcagcac ggccgccacc gccggatgaa
     1261 aatcgacaat tgtctcca
//
LOCUS       DATCBB010000723         1655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_412894, whole genome shotgun sequence.
ACCESSION   DATCBB010000723 DATCBB010000000
VERSION     DATCBB010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412894"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..539
                     /gene="hfq"
                     /locus_tag="VII37_08055"
     CDS             225..539
                     /gene="hfq"
                     /locus_tag="VII37_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY5069727.1"
                     /translation="MENKPAQNIQDSFLNTARKERNTITIYLLSGVKLTGRIRSFDKY
                     SIVLDANSQEQLIFKHAISTVAMGRTSHAHGDKAGSPASAGSELGSSSSEPSATTSAN
                     EA"
     gene            609..>1655
                     /gene="hflX"
                     /locus_tag="VII37_08060"
     CDS             609..>1655
                     /gene="hflX"
                     /locus_tag="VII37_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY5069728.1"
                     /translation="MNSADATARERAFLVGLDYRKRASKAKSAGSSHATSGKGATNSP
                     PSKAPPIPEFTAEESLDELRSLAASAGADVAGEFLQRRERPDAATLIGKGKLQEIAGA
                     AASVSADLLLFDHDLTASQQRNIEKVVGIRVIDRTQLILDIFARHARTREGQLQVELA
                     QLEYMLPRLSGRGIEMSQLGGGIGTRGPGETQLETDRRKIFRRVRHVKQQLENVRRVR
                     AQQRQRRESVPVSTIALVGYTNAGKSTLFNAMTQAGVLESSKMFATLDPTIRGISLPS
                     RREVLLSDTVGFIRNLPHTLVSAFRATLEELQRADLILHVSDASSPLSAEQDAQVEKV
                     LQELEVEAKPRLRIV"
BASE COUNT          374 a          462 c          476 g          343 t
ORIGIN      
        1 actgcgccac tacctgcgaa acccccagct tgtcccaatt ccgctccttg cagttgaaaa
       61 atactctggt agcccgtatt gtacgaatgt tatcccaaag gctttgccgt gctccggcgt
      121 tcggtgagcc ggcgatatcc tttgaaggta actgggccga gtctgggagt ttgagtattt
      181 cgcggtagag tggctttgct gccagaagcc caaggagatc ggcgatggag aataagccgg
      241 cgcagaacat acaggattct ttccttaata cggctcgcaa ggaaagaaac actatcacta
      301 tttatctttt gagcggcgtg aagcttacag ggcgcattcg ctcgttcgat aagtactcga
      361 tcgtgcttga tgcgaatagc caggagcagc ttatctttaa gcacgccatc tccacagtgg
      421 cgatgggtcg aacgtctcac gcgcacggcg acaaagcggg gtctcccgcg tccgctggaa
      481 gcgaattagg ttcatcatcg tctgaaccat cggccaccac ctccgcaaac gaagcatagg
      541 catcctaaaa cttgccaggc ccacaatcca atcggtcacg caaggcgtct ctctccaggt
      601 cttccaattt gaattcggca gatgcgactg cgcgggaacg cgcttttctg gtggggctgg
      661 attatcgcaa gcgagcatcc aaggcaaagt ccgcgggatc ctctcacgca acgagtggga
      721 agggcgcgac gaattccccg ccgtccaaag ccccgccgat cccggaattc accgcggagg
      781 aatccttgga cgaactgcga tcgcttgccg ccagcgctgg cgccgatgtt gcgggcgaat
      841 ttctgcaacg ccgtgagcgg cccgatgccg cgacattgat agggaaggga aagctgcagg
      901 agattgccgg cgcagccgca tcggtctcgg cggatcttct actattcgac cacgatctga
      961 cggcttctca gcagcgcaat atcgaaaagg ttgttggtat ccgggtgatt gatcgaaccc
     1021 aactgatcct ggacattttc gcgcggcacg cccgaacccg cgagggccag ttgcaagtcg
     1081 aactggcgca gttggaatac atgcttccgc gattgagtgg acgcgggata gagatgtccc
     1141 aactgggcgg aggaattggg acgcgcgggc cgggagaaac ccagcttgag actgaccgta
     1201 gaaaaatttt ccgccgtgtt cgccacgtca agcagcaatt ggaaaacgtg cggcgggttc
     1261 gcgcgcagca gcggcaacga cgagagtccg tacctgtttc cacgattgca ctcgtcggat
     1321 atacaaacgc ggggaagtcc acactattca acgcgatgac gcaggctgga gttttggagt
     1381 cgtccaaaat gttcgccacg ctggacccga ccattcgggg gatttcgttg ccttcgcgcc
     1441 gcgaggtact gctttcagat acggtgggat ttattcgcaa tctgccgcat acactcgtgt
     1501 ccgcttttcg cgccactctg gaagaattgc aacgcgcgga tctcatattg cacgtctccg
     1561 acgccagcag cccactctca gccgaacagg atgcgcaagt ggaaaaggtt ctgcaagaac
     1621 tggaagttga agcgaaaccg cgactgcgga tcgtg
//
LOCUS       DATCBB010000724         1663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_249489, whole genome shotgun sequence.
ACCESSION   DATCBB010000724 DATCBB010000000
VERSION     DATCBB010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249489"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VII37_08065"
     CDS             <1..185
                     /locus_tag="VII37_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069729.1"
                     /translation="GVEGLSRVITRKSAERILAHLNAPVEQPDGADGAAAHSDANERN
                     MGENGGTDSKVTTTTS"
     gene            237..527
                     /locus_tag="VII37_08070"
     CDS             237..527
                     /locus_tag="VII37_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY5069730.1"
                     /translation="MADNVGSKVSYFLVGLGVGALVGVLFAPKSGEDTREYLAGKADE
                     GKDYAQRKARELRERADELIERSKDVASRKKDSLSAAVDAGREAYLREASKS"
     gene            590..1093
                     /locus_tag="VII37_08075"
     CDS             590..1093
                     /locus_tag="VII37_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEY5069731.1"
                     /translation="MEFWLEVFSIVAAIALVVQVIILGALFFELKRTTEKVNKLVSDM
                     QTRVGPILSRVQLLLDDTQPRISEMVADASHVVYLARGQAQKMDRVLSEESDRLRGQL
                     IHADRILSGALEAVEETGAKVRQGFSGPMRRASAVVTGIRVGLDLLRSRRVPRRPEEQ
                     HEEELFI"
     gene            complement(1167..>1663)
                     /locus_tag="VII37_08080"
     CDS             complement(1167..>1663)
                     /locus_tag="VII37_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5069732.1"
                     /translation="LFDFGCYGADLMTVLMHGHPPVSVTAVTQTDKPEIYPRVDDDAT
                     VILRYPKAQAVLMPSWNWSFARKDMEVYGNAGYAITVGPDRVRLRYHGEKAEPDAPLT
                     APPLGGAQRDSLSYLAAVLRGEVQPHGDLSSLETNLIVMQILEAALKSAQSGKTVMLT
                     PLPQ"
BASE COUNT          376 a          374 c          544 g          369 t
ORIGIN      
        1 cgggcgtcga gggcttgagc cgggttatta cgcggaaaag tgcggaaaga attctggcgc
       61 atctgaacgc accagttgag cagcccgatg gagctgatgg tgcggccgcg catagcgatg
      121 cgaatgagag aaatatgggg gaaaacggcg gaactgatag caaagtgacg acgactacca
      181 gttagcctgt attgaacctg acttggtgct attatacggt cacaacggag gtcatcatgg
      241 ctgataatgt tggttccaag gtgagttatt ttttggtggg attgggcgta ggcgcgctgg
      301 taggagtttt gtttgctccc aagtcgggtg aggatacgcg cgaatacctg gcgggcaaag
      361 ccgatgaggg caaagattat gcgcagcgta aggcgcggga actgcgcgag cgcgcggatg
      421 aactgattga acgcagcaag gacgtagcat cgcggaagaa ggattctctc tcggcggcgg
      481 tagatgcggg gcgcgaagcg tatctgcggg aagcgtcaaa gtcttaacgg aatttaaggg
      541 acgaagttaa caaaatttcc gctcagaaat gtgggcggct ggggcgctca tggaattttg
      601 gcttgaggtg ttcagcattg tcgcggccat cgctctggtg gtgcaggtga tcattctggg
      661 tgcgcttttt tttgagttga agcgcaccac ggaaaaagtg aacaaactgg tcagcgatat
      721 gcagacgcgc gtggggccga tcttgagccg cgtgcagttg ctgctggacg atacgcagcc
      781 gagaatctca gagatggttg ctgatgcctc gcacgtcgtt tatctggcac gagggcaggc
      841 gcaaaagatg gaccgcgtat tgagcgagga atcggaccgg ttgcgcggac agttgattca
      901 tgcggaccgc attttgtccg gcgctttgga agcggtggaa gagactgggg cgaaggtgcg
      961 gcaggggttt tcggggccga tgcgcagagc ttccgcggtg gtgaccggga ttcgcgtcgg
     1021 gttagatttg ttgcggtcga ggcgggtgcc gcgacgaccg gaagagcagc atgaagagga
     1081 attgttcatc tagggtagga aattaccgga actaaagaag tcgatcaaac ttcgttacaa
     1141 actcacagaa aaatctaaaa gaaaaattat tgaggcagtg gcgtcagcat tacggttttg
     1201 ccgctctgtg ctgatttcag ggccgcttcc agaatctgca tgactatgag atttgtttcc
     1261 agcgatgata agtctccgtg aggctgaacc tcgccgcgca gtactgctgc cagataacta
     1321 agtgaatcgc gttgcgcgcc gcctagtggt ggggcggtaa gcggcgcgtc tggctccgct
     1381 ttctcaccgt gatatcgcag acgtacacgg tctggtccca cggttatggc atagccggcg
     1441 ttgccgtaga cttccatgtc cttccgtgcg aaagaccagt tccacgaggg cataagcacc
     1501 gcttgggctt tcgggtaacg caaaatcacg gtcgcatcat cgtccacgcg aggataaatt
     1561 tccggcttgt ccgtttgcgt cactgctgtg acgcttacgg gcggatggcc gtgcattagt
     1621 acggtcatca ggtctgcgcc gtaacaccca aagtcaaaca atg
//
LOCUS       DATCBB010000725         1157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_153863, whole genome shotgun sequence.
ACCESSION   DATCBB010000725 DATCBB010000000
VERSION     DATCBB010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153863"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..771
                     /locus_tag="VII37_08085"
     CDS             121..771
                     /locus_tag="VII37_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069733.1"
                     /translation="MRTRCSQIPLALLIAAVLLCGHPVALAQQKPGEKPKRISKTIWN
                     FDGGVFLETDGSLSENTCFRLAGHMMDKGFFDNLKRIDDNQGTRYVRGKEIVTEFPER
                     LKLLFVIHDLPCPSQVPNSKGREYLTREMMSTLHLSLFWKRGVDLRPAEKFKVKFFSV
                     EPVQPYATELAKELPKRFQWAYELEIESAGIPLSDSLVLIFRRDDGRIAARVAARL"
     gene            complement(818..>1157)
                     /locus_tag="VII37_08090"
     CDS             complement(818..>1157)
                     /locus_tag="VII37_08090"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY5069734.1"
                     /translation="DVAVLGGFQVSEKGDLANWKVPGARGSGGIGGAMDIAAGAKILI
                     VCMEHTTKEGAPKIVKQCTYPLTGLACVDTIVTDLAVIDVRPQGLLLRETAPGWTKKE
                     IQEVTGAPLL"
BASE COUNT          258 a          333 c          296 g          270 t
ORIGIN      
        1 tccattgcca gcagttcgca tccacctagc cgccgaagtg cgtccctcgc gagttgacag
       61 cgctgctcgc gacacacgta cactagtcga gccgataaac tttccgttta actacttgcg
      121 atgcgcaccc gttgttctca aattccgctg gcattattga tcgccgcagt gcttctctgc
      181 ggccatccgg tagcgctggc gcaacaaaag cccggggaga aaccaaagcg catttcaaaa
      241 acaatctgga attttgatgg cggcgtattc ctcgaaacag acggcagcct gagcgaaaac
      301 acttgctttc ggctcgccgg ccacatgatg gataaggggt tctttgataa tttaaaacgc
      361 atcgacgata accagggcac caggtatgtt cgcgggaaag agatcgtgac tgaatttcct
      421 gagcgcctga aactgctgtt tgttatccat gacctaccct gtccatctca ggtcccaaac
      481 tccaaaggcc gcgaatacct tacgcgcgaa atgatgagca cgctgcatct ttcgctcttc
      541 tggaagcggg gagtcgattt gcgcccggca gaaaaattta aagttaagtt tttctcggtc
      601 gagccggttc aaccctacgc cacggaactt gccaaggaat tgccgaagag attccaatgg
      661 gcttatgaac tggagattga aagtgccgga attcccttga gcgacagtct ggtcttgatt
      721 ttccgtcgcg acgacggccg catcgccgcg cgtgtggcgg cgcgcctcta gtcgctgaaa
      781 agtgccggcc ccgttcctgt aggaatttta gcttgggcta taaaagtggc gcaccggtta
      841 cttcctgaat ttccttttta gtccagccag gagcggtctc gcgtaacagc agtccttgcg
      901 gtcgtacatc aatcaccgcc aaatcggtaa cgatggtgtc cacgcacgcc agtccggtga
      961 gcgggtaggt gcactgcttc acaattttcg gcgcgccctc ttttgtagtg tgctccatgc
     1021 acacaatcaa tattttcgcg cctgccgcga tatccatggc gccgccgatg ccgccggaac
     1081 cgcgcgcgcc aggaactttc cagttagcca ggtcaccttt ttccgagact tgaaatccac
     1141 caaggaccgc gacatcc
//
LOCUS       DATCBB010000726         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_23434, whole genome shotgun sequence.
ACCESSION   DATCBB010000726 DATCBB010000000
VERSION     DATCBB010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23434"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VII37_08095"
     CDS             <1..472
                     /locus_tag="VII37_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5069735.1"
                     /translation="VLREHLINHARTFIFSTAMPPYMAEQIRAALRLAITMDRERAEL
                     IARAADFAKSLLREGWESMGSATQIVPAVIGENDAALAAAEYLQHSGFAVRAIRPPTV
                     PQGTARLRFSLTHKINGTELQRLAEVLCAWRAQQQHATSHATSQATPMTMAGTA"
     gene            469..1104
                     /gene="bioD"
                     /locus_tag="VII37_08100"
     CDS             469..1104
                     /gene="bioD"
                     /locus_tag="VII37_08100"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY5069736.1"
                     /translation="MRHHFFITGTDTGVGKTTLAALLCAALDAVYWKPIQTGTLEGSD
                     RVTVMRLAGLGTDRTLEEVYKFVPPVSPDLAARWAGVEIDLARINLPTTLVNDWLIVE
                     GAGGVLVPINAKQFMLDLMAKFKLPVILAARSGVGTINHTLLTLAALHAAELPVHGVV
                     LIGDQNRENRETIERFGHVRVIGEIPRLPNLHRTSLMQVFMNNFDRAAFSQ"
     gene            1111..2409
                     /gene="bioA"
                     /locus_tag="VII37_08105"
     CDS             1111..2409
                     /gene="bioA"
                     /locus_tag="VII37_08105"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY5069737.1"
                     /translation="MSSPLRIWHPFTNAAMDAPPLHVVRAERVYLYTKDGRRILDAVS
                     SWWVNLHGHANPRIAEAIAEQARKLEHVIMAGFTHDAAEELASRLRKWLPAQMTHLFF
                     SDDGSTAVEVALKLAVQHFSNSGRAQKSEIVALTHGYHGDTAGAMSASDDSLFNKPFQ
                     SMRYPIHRVHSAYCHRCPVGLTRATCHIECVQQLATLLEENHERIAAVIVEPLLQGAG
                     GMIVHPVEFLEKVRERCTRYDVLLIADEVLTGFGRTGKMFACELADVIPDLMCLSKGI
                     TGGFLPMGATVCSDRVEAPFRSKDRLHTFYHGHSYTGNALACAAANASLQIFDDEPVF
                     ERIEMISAVNTERLEQFRKLPQVADVRQIGTMGAIELRAEDAGYLSSMRTKLYQFFID
                     RGVLLRPLGNVVYVLPPYVITREELHFVYDVILQGIEALL"
     gene            2525..>2821
                     /locus_tag="VII37_08110"
     CDS             2525..>2821
                     /locus_tag="VII37_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069738.1"
                     /translation="MVISLHTWKQKIVAFAGSLGAPGLFLISFLDSSVLTFPVINDLL
                     LIELSMQHHSRMPLYALMAASGSTLGCILLYFIARKGGEAFFHSRAGKHGGAVRH"
BASE COUNT          591 a          818 c          773 g          639 t
ORIGIN      
        1 ggtgctgcga gaacatttga tcaatcacgc ccgtacgttt atcttcagca ccgccatgcc
       61 gccgtacatg gcggagcaga ttcgcgcggc gctgcgtttg gcaatcacca tggaccgcga
      121 gcgcgcggaa ttgattgcgc gtgcggcgga tttcgcaaaa tccctgctac gcgaaggctg
      181 ggaatcgatg ggcagcgcga cgcaaattgt tcccgcggtt atcggcgaaa atgacgccgc
      241 cttggccgcg gccgaatatc tgcagcactc cggctttgcc gtcagagcga ttcgcccgcc
      301 gaccgtgcca caaggcaccg cgcggctccg tttttcattg acgcataaaa tcaacgggac
      361 tgaattgcag cgactggccg aggttttgtg cgcgtggcgc gctcaacaac agcacgcgac
      421 ttcgcacgca acttcgcaag cgactcctat gaccatggcg ggaaccgcat gaggcatcat
      481 ttttttatta ccggaacgga caccggcgtg ggcaagacca ccctcgctgc gttactctgc
      541 gccgcgctag atgcggtcta ttggaaaccc atacaaaccg gcacgctgga gggctccgat
      601 cgcgtaacgg tgatgcgcct cgccggcctc ggcaccgatc gcacgctcga agaagtctac
      661 aaatttgtgc ccccagtttc gcccgacctg gccgcgcgct gggctggcgt ggaaatcgat
      721 ttggcgcgta ttaatttgcc gaccactctg gttaatgact ggctgattgt cgaaggcgcg
      781 ggcggcgtgc tggtgcccat caatgcaaaa caattcatgc tggacctgat ggcaaaattc
      841 aagctgccgg taattcttgc cgcgcgaagt ggcgtgggca ccatcaatca cacgctactc
      901 acgttggccg ccttgcacgc tgcggagctt cccgtgcacg gcgtggtttt gattggcgat
      961 cagaatcgcg aaaatcgcga aaccatcgag cgcttcggac acgtccgcgt catcggagaa
     1021 attccacgct tgcccaattt gcaccgcact tcgctgatgc aagtgttcat gaataatttc
     1081 gaccgcgcgg ccttttccca ataagacaaa atgagttcgc cgctgcgcat ctggcacccc
     1141 ttcaccaacg ccgcaatgga tgcacctccg ctgcacgtgg tgcgcgcgga acgcgtttat
     1201 ctctacacca aagacgggcg gcgcattctg gatgcggttt cgtcctggtg ggtgaacttg
     1261 catggacacg ccaatccgcg catcgccgaa gccatcgcgg aacaggcccg caaattggag
     1321 cacgtcatca tggcgggctt cacgcacgac gccgccgagg aattagcctc gcgcttgcgc
     1381 aagtggttgc ccgcgcaaat gacgcacctg tttttctccg atgacggctc cacagccgtg
     1441 gaagtcgcat tgaaactcgc ggtgcagcat ttcagcaaca gcggccgtgc gcaaaaatcg
     1501 gagattgtgg cgctcacgca tggctatcac ggcgataccg ccggcgccat gtccgcgagc
     1561 gacgattcgc tgttcaacaa accatttcag tccatgcggt acccaataca ccgcgtgcat
     1621 tccgcgtact gccaccgttg tccggtgggt ctcactcgcg caacttgtca catcgaatgc
     1681 gtgcagcaac tcgcaacatt gttggaggaa aatcacgaac gcatcgccgc cgtcattgtt
     1741 gaaccgttgc tgcaaggcgc aggtggaatg attgtgcatc ccgtggaatt cctggaaaaa
     1801 gtgcgcgagc ggtgcacccg ttacgatgtt ttgctgattg ccgatgaagt gctgaccggt
     1861 ttcggccgca ccggaaaaat gtttgcctgc gaacttgccg atgtgattcc agatttaatg
     1921 tgtctgtcaa aaggaatcac cggcggattt ttacccatgg gcgcgacggt atgcagcgac
     1981 cgcgtagagg ctccgtttcg cagcaaagac cgcttgcaca ccttttatca cggacattct
     2041 tataccggga acgctctagc ctgcgcagcg gccaacgcca gcctgcagat tttcgacgac
     2101 gagccggtct ttgaacgcat cgaaatgatt tctgcggtta acaccgagcg gctggagcaa
     2161 ttccgtaaat tgccgcaggt cgcggatgtg cggcagatcg gaaccatggg agcaattgaa
     2221 cttcgcgctg aagacgccgg atatttatcg agcatgcgca ccaagcttta tcaatttttt
     2281 atcgaccgcg gtgtgttgct gcggccgctt ggaaacgtag tttacgttct ccccccgtat
     2341 gtaatcaccc gcgaagaatt acatttcgtc tatgacgtaa ttttgcaggg catcgaggcc
     2401 ctgctttgac caaagcggca gccggcctcg ccatctatca gcgttttttc tttttcgcac
     2461 taaggttttg agaaccaaaa attgccagca agagtgttag tgtgacttgc gcgaggtgct
     2521 gtacttggtg atttcgctcc atacctggaa acaaaagatc gtcgcttttg ctggcagctt
     2581 aggcgctccc ggactttttc tcatttcatt tctggattcc tctgttttga ctttccccgt
     2641 cataaacgat ctcttgctga tcgaactatc catgcaacat cattcgcgca tgccgcttta
     2701 cgctttgatg gcggcgtcgg gctctacact gggatgcatt ctgctgtatt tcatcgcccg
     2761 caaaggcggc gaagcttttt ttcacagcag agcagggaag cacggcggcg cagtccgcca
     2821 c
//
LOCUS       DATCBB010000727         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_87757, whole genome shotgun sequence.
ACCESSION   DATCBB010000727 DATCBB010000000
VERSION     DATCBB010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87757"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VII37_08115"
     CDS             complement(<1..864)
                     /locus_tag="VII37_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5069739.1"
                     /translation="MRLAEICVKYPVFTVMLILFLVTLGIFSYRGLAVDLFPKADPAT
                     VNVEVRLPGATPEEVITGVVLPLEDAISSVSGIDEITVYSTEGYAEITCTFILEREIE
                     GAAQDIREKVAAATDRLPRDTLAPVITKQDPQSDPIMTVLVTGSRSRRELTEIADKQV
                     RRAIQTVDGVGSVDLSGGQGREIRVLLDARRLTSHSFTVLDVRDALRRENIEAPGGRM
                     VVGPQELGLRTLGRVTSAEQFGEIVIGTRGGAPVRVRDVAQVEDGAAELRTWSAFFRK
                     NVPGEDVVTVQV"
     gene            complement(895..2154)
                     /locus_tag="VII37_08120"
     CDS             complement(895..2154)
                     /locus_tag="VII37_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5069740.1"
                     /translation="MTQQLPCHKNSPAKLGYLAILATALSLWACSKSTSSSVLGDASN
                     PTPVNVYTVAEETAQRHVQATGSLFPFEESVVSAQVEGRISEVLADIGDSVKEGQALV
                     ILDPQELQFEVDRQRGIVRQVRAQLGIGPGDPPPADPKTLPSVQRAEADRFEADHKYN
                     RAREMFEGKLISQQQLDEAASRYQSTKATYDVALQEVDRLKALLVSSEASEKLAEKKL
                     GDATIRAPFPGAVKTRDVHPGEFLHVQSPVMVLVRTDRLRARLAVPERWAGWVKDGVV
                     VEVHVEAFPNETFQGKVSRINPSVSQDSRTFDAEALLVNADGRLKPGFFVQASIPSEK
                     EEKTIFLPERAVNYRYGVYKVFLLHGNQVSEHQIQPAGQTEDKRGRRFEVAEGLKPGD
                     RVAVAVTGELQDGITVKEQPESSTPTK"
BASE COUNT          497 a          762 c          658 g          528 t
ORIGIN      
        1 cacctgcacg gtcaccacgt cctcaccggg aacgttcttg cggaagaacg ctgaccaggt
       61 gcgtagctct gcggcgccat cttccacctg ggcgacatcc cgcacacgca caggcgcgcc
      121 gcctcgcgtg ccgatgacga tttcgccgaa ttgctcagcg gaggtgacgc gtccaagagt
      181 acgcaggccc aattcctgcg gaccaaccac catgcggccg ccaggcgctt caatattttc
      241 ccgccgcaac gcgtcccgca catcgagcac cgtgaagctg tgcgaggtca atcgtcgcgc
      301 atccagcaaa acgcgaatct cgcggccttg accgccactg agatctacgc tgccaacacc
      361 atcaacggtc tggatcgccc gccgcacctg cttgtccgcg atctccgtta gttcgcggcg
      421 gctgcgcgat cccgtcacca gcacggtcat gatcggatcc gactgcggat cctgcttggt
      481 gataaccggc gccagcgtgt cgcgcggcaa acgatccgta gcggcggcta ctttttcgcg
      541 gatatcctgc gcagcccctt cgatctcccg ctccaggata aatgtgcagg tgatctccgc
      601 atatccttcc gtggagtaca ccgtgatttc atcgatgccg ctgaccgaag agatggcatc
      661 ttcgagcggc aaaaccacgc cggtaatcac ttcctccggt gtggcgcccg gcaggcgcac
      721 ttccacattg accgtggcgg gatcggcttt ggggaaaaga tctaccgcga ggccgcgata
      781 cgaaaaaatt cccagcgtga ccagaaacag aatcagcatc acggtgaaga ccggatactt
      841 cacgcagatt tcagcaagtc tcatagaacc gtttcacccg gtctgttcag gaacctattt
      901 cgtgggcgtg gaactctcag gctgttcttt tacggtgatg ccgtcctgca actcgcccgt
      961 aaccgccacg gccacacggt cgcccggttt caggccctcg gcaacttcaa aacgacggcc
     1021 gcgcttatct tcggtctgtc ccgccggctg aatctggtgc tcggaaacct ggttgccgtg
     1081 cagcaagaaa actttgtaaa cgccgtagcg gtaatttacc gcgcgctccg gcaggaagat
     1141 ggttttctct tccttctcgc tgggaatgga cgcctgcacg aaaaatcccg gtttcaatcg
     1201 gccatccgca ttgactaaca gcgcttccgc atcaaaggtg cgcgagtcct gtgaaaccga
     1261 tggattgatg cgggaaactt tgccctgaaa agtttcattc gggaacgctt ccacgtggac
     1321 ttcgacgaca actccgtctt ttacccagcc tgcccaacgt tccggcactg cgagccgcgc
     1381 acgcagccgg tccgtccgca ccagcaccat caccggactt tgcacatgca gaaattcacc
     1441 agggtgaacg tcgcgggttt ttaccgcgcc gggaaatggt gcgcggatgg tggcatcacc
     1501 taattttttc tcggcgagct tctcactggc ctcgctggaa accagcagtg ccttcaaccg
     1561 gtccacttcc tgcaacgcta catcgtaagt agccttggtg ctttgatagc ggctggcggc
     1621 ttcatcgagc tgctgctgcg aaattagctt gccctcaaac atttcgcgcg cgcggttgta
     1681 cttgtggtcc gcttcgaaac gatccgcctc ggcgcgctgc acgctgggga gcgttttcgg
     1741 gtccgcaggc ggcggatcgc cgggcccgat acccagttgg gcgcgaactt gccgcacgat
     1801 gccccgttgg cggtcaactt cgaattgcag ttcttgcgga tcgaggatca ccagagcctg
     1861 gccctcttta accgagtctc cgatatcggc taaaacctcc gaaatgcggc cctccacttg
     1921 tgcgctgacg acgctttctt caaacgggaa gagcgagccg gtggcctgta catggcgctg
     1981 agcagtttcc tcggcaacgg tgtacacgtt gacaggcgtc gggttgctgg cgtcgcctaa
     2041 aacgctgctc gaggtggatt tactgcatgc ccacaaagat aaagccgttg ccaggatcgc
     2101 taaatatccc agcttagccg gcgagttctt atgacacgga agttgctgag tcatgaccac
     2161 cgaatgcgtc gtagcccctg tagtacttcg acgtctcaag atgcctcttg tcacctgcgg
     2221 ataggctaca aatcgattta agttcacgcc agattatgaa cgcaaaaaag tattagtcga
     2281 agcagaattt gtccctgaga ccgcaggcac gagttccggc gtaatatctg gaaaccctat
     2341 cggttcaatc gattcgacct gaatcgcgtt cgctcccgag gtccttactc tgtcacagct
     2401 tttgctcgac gcctctggcc aacgcgctct gagaaggcca atttt
//
LOCUS       DATCBB010000728         1011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_87798, whole genome shotgun sequence.
ACCESSION   DATCBB010000728 DATCBB010000000
VERSION     DATCBB010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87798"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VII37_08125"
     CDS             complement(<1..311)
                     /locus_tag="VII37_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEY5069741.1"
                     /translation="MNKEKFKPKKVVLAYSGGLDTSIIIPWLKENYDCEVIAMIGDVG
                     QQEDLEAARKKALDTGASTALVEDLREEFVRDYIWPTLRAGAVYEHTYLLGTSMARPV
                     IA"
     gene            complement(464..>1011)
                     /locus_tag="VII37_08130"
     CDS             complement(464..>1011)
                     /locus_tag="VII37_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069742.1"
                     /translation="SDKFHPCQGLADFLTIEEKVGSLRGFKLAYVGDGNNVCHSLIFL
                     AARLGVHLRIATPPGYAPLPEVIQEAKRVARETRAKIELFTDPREAVAGAQGVYTDAW
                     TSMGFEAEGDVRETVFQPYQVNTELMALAAPDALFLHCLPAHRGHEVTAEVLDGPQSV
                     VLDQSENRMYVQKAILQTLFS"
BASE COUNT          200 a          312 c          283 g          216 t
ORIGIN      
        1 gcgatcaccg gacgcgccat cgaggtgccc agcagatacg tgtgctcgta aaccgctccg
       61 gcacgcagcg tgggccagat gtagtcgcga acaaactctt cgcgcagatc ttccaccaat
      121 gccgtggacg ctcccgtgtc cagagctttc ttacgcgccg cttccaaatc ctcctgctgg
      181 ccgacatcgc cgatcatcgc gatgacttcg caatcgtagt tttccttgag ccagggaatg
      241 atgatggacg tgtcgagccc gccggaataa gcgagtacga cctttttcgg tttgaatttc
      301 tctttgttca cgattcctct ctcaaatgat ggtgatcatt attgccgcag acggcgcgcg
      361 atgcacacca atatcgcggg cctttcgaat acgccgaaat ctgtggctgt gattgtacct
      421 gcgcttgcgc ggcgcgagat caataccaat cgggccgccg cgatcaactg aagagcgtct
      481 gcagaatggc tttctgcacg tacatgcggt tttccgattg atcgaggacc actgattgcg
      541 gcccgtcaag cacttccgcg gtgacttcat gtccgcggtg cgccggaagg cagtgcagga
      601 ataaagcatc cggcgcagcc agcgccatca gctccgtgtt cacctgataa ggctggaaca
      661 cggtttcccg cacatcccct tccgcttcaa agcccatgct ggtccacgcg tcggtgtaca
      721 cgccttgcgc gccggccacc gcctcgcgcg ggtcggtgaa cagttcgatt ttcgcgcgcg
      781 tttcccgcgc cacgcgcttg gcttcctgaa ttacctccgg cagcggggcg taacccggcg
      841 gagtggcgat gcgtagatga acgcccaggc gcgccgccag aaaaatcaac gaatggcaaa
      901 cgttgttgcc gtcgcccaca taggccagct tgaatccgcg cagcgagccg actttttctt
      961 cgatggtcag aaaatcggcc agcccctggc agggatgaaa tttgtcggag a
//
LOCUS       DATCBB010000729         1606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_87834, whole genome shotgun sequence.
ACCESSION   DATCBB010000729 DATCBB010000000
VERSION     DATCBB010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87834"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VII37_08135"
     CDS             complement(<1..722)
                     /locus_tag="VII37_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069743.1"
                     /translation="MFLEIFREAWAALGRNPVRSLLTMTGISWGIVAVTLLLSYGSGF
                     RNVLMYTFEVFGKGAVVCWPGTTSEQAGGERAGKAVRFEQEDADWVKAQSPLVKRVTL
                     ETVKFKGISHEERLSDTAIRGVYPEYGEMRNEVPLEGRWISQEDIAERRRVAFLGAIL
                     RKKLFSGTPAIGETVRINGVKFTVIGTMDTKFSDSNYFTSDDESAFIPYTAASDLWDA
                     RYASVMLFEPVAPNFEANAMQQV"
     gene            complement(725..>1606)
                     /locus_tag="VII37_08140"
     CDS             complement(725..>1606)
                     /locus_tag="VII37_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069744.1"
                     /translation="MPEFTEIRSIAVAQGRFYNHEDNSAARNVAFLGSDTKKQLFSDH
                     EAVGQQVWMNEIPYSVIGVMKSKDQNSSYDGMDTRKIFIPYNTMRRDFPTKAPGVEHT
                     IDRLLVAPKSLETHPDCLRQIRRSLGRLHNFDPRDKEAAGIWDTVKNAEANRMIIVGM
                     ELFMGAVGIATLFLGGLGVMNVMLVSVRERTREIGVRMALGATRKSILRQFFMETVIV
                     VALSGGTGLLVSYGFCALVNLLPMPPFFAGLLASWKLGALSVTLLGLISVLSALYPAN
                     RAASVDPIEALRFEAGG"
BASE COUNT          355 a          506 c          403 g          342 t
ORIGIN      
        1 acctgctgca tggcattggc ctcaaaattt ggcgccacgg gctcaaataa catcacgctg
       61 gcataacgcg catcccacaa gtccgaagct gccgtatacg gaatgaaggc ggactcatcg
      121 tcgctcgtaa aataattgct gtcggaaaat ttggtgtcca tcgtgccgat gaccgtaaat
      181 ttcacgccgt tgatgcggac cgtttcgcca atcgccggtg tcccgctgaa taatttctta
      241 cgcagaatcg cgccgaggaa cgccacccgc ctgcgctccg cgatgtcttc ctgcgaaatc
      301 catcgccctt cgagcggcac ctcgttgcgc atctcgccat attcgggata aacgccgcgg
      361 atggcggtat ccgacaagcg ctcctcgtgc gaaatgccct tgaacttcac ggtctccagc
      421 gtcacgcgct ttaccagcgg cgattgcgcc tttacccagt cagcatcttc ttgttcgaag
      481 cgcactgctt tgccggcgcg ttcgccgccg gcctgctcgc tggtggtgcc cggccagcaa
      541 actaccgcgc ctttgccaaa tacttcaaag gtgtacatca gcacattacg aaatccggag
      601 ccgtaactca gcaacagcgt taccgccacg atgccccagg aaattccggt catggtcagc
      661 aggctgcgca ccggattgcg tcccagcgcg gcccacgctt cgcgaaatat ttcgaggaac
      721 atagttagcc gcccgcctca aaccgcagcg cttcgatggg atccaccgac gcagcccggt
      781 tcgcgggata gagcgccgat agaacggaga tcaaccccag taaggtcacc gacagcgctc
      841 caagcttcca acttgccagg agcccggcga aaaatggcgg catcggcaac aggtttacca
      901 gcgcacaaaa tccatacgac accagcagcc ccgttccgcc gctgagcgcc accacaatca
      961 ctgtctccat aaaaaattgc ctcagtatcg atttgcgcgt cgcgcctaac gccatgcgca
     1021 caccaatttc ccgcgttcgt tcacgcacgg acacaagcat tacgttcatc acgcccagcc
     1081 cgcccagaaa cagggtggca attcccaccg cgcccatgaa gagttccatg cccacgataa
     1141 tcatgcggtt ggcttccgcg ttttttaccg tgtcccagat tcccgcggct tctttgtcgc
     1201 gcggatcgaa attgtgcaaa cgccctaagg agcgtcgaat ttgacgtaaa caatccggat
     1261 gcgtctcgag cgatttcggc gcaaccagca ggcggtcaat ggtgtgctct acgcccggcg
     1321 ccttggtggg aaaatcccgg cgcatggtgt tgtaaggaat aaaaatcttg cgagtgtcca
     1381 tgccatcgta gctggagttt tggtccttgg atttcattac gccaatcacg gagtagggaa
     1441 tctcgttcat ccacacttgc tggcccaccg cttcgtgatc cgaaaaaagt tgtttcttcg
     1501 tgtcactgcc caaaaacgcg acgttacgcg ctgcggaatt atcttcgtga ttgtaaaagc
     1561 gcccttgtgc caccgcgatg ctacgaatct ccgtgaattc cggcat
//
LOCUS       DATCBB010000730         1017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_347636, whole genome shotgun sequence.
ACCESSION   DATCBB010000730 DATCBB010000000
VERSION     DATCBB010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347636"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..>1017)
                     /locus_tag="VII37_08145"
     CDS             complement(1..>1017)
                     /locus_tag="VII37_08145"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY5069745.1"
                     /translation="SAGAVPGPVCYGRGMNPTVTDAHAVLGHLGDAGLLGGSFSLDIP
                     RARAEMQNVVKQSDERFRTVEAFAQGIIAVANAVMEKAIRVISVERGHDPRDYTLIAF
                     GGAGGLHACDLAAALEMRGVLIPVFPGGLSALGILQADVVKELSRTVLLHAEELLEES
                     RQLKGIVKRLGDEAMRVLHAEAFSPDKMRFQYSLDMRYLGQAYELNIPFSAVGHTGSV
                     GHDVIGDFHRAHEMRYGYHHRNKKVEIVNVRCRATGITEKPPSQKIAPRSRTKPLLPE
                     QTMGTTFYERKRETILYRRDNLHAGDILSGPAIVAEYSATTLVPPEWSARVNGYGQLL
                     LTPK"
BASE COUNT          231 a          283 c          277 g          226 t
ORIGIN      
        1 ttattttgga gtcaacaaaa gttggccata gccgttcacc cgagcgctcc actccggcgg
       61 aacgagggta gtggcactgt actccgccac aattgccgga ccggaaagaa tgtcgccggc
      121 gtggaggtta tcgcgccggt acaaaatggt ttctcgcttg cgctcgtaaa acgtagtccc
      181 catcgtctgc tctggtaaca aaggcttggt tcgcgaacga ggagcaattt tctggctcgg
      241 gggcttttcc gtgattccgg tagcccgaca gcggacgttt acgatttcca cttttttgtt
      301 tctgtgatgg tagccgtaac gcatctcatg agctcgatga aaatcaccga tcacgtcatg
      361 accaactgag ccagtgtggc cgacagcgct gaacggaata ttgagttcgt aagcctgccc
      421 cagataacgc atatcgaggg aatactggaa gcgcattttg tctggagaga atgcttctgc
      481 gtgaagaact cgcattgctt cgtcgccaag gcgcttcaca atacctttta attgtcgcga
      541 ttcttcaagt agttcttctg cgtgaagcaa gaccgtgcgg gacaattctt taacgacatc
      601 ggcttgcaga attccaagtg ccgacaaacc gccaggaaaa acaggaatca acacgccgcg
      661 catttcgagc gcggcagcga gatcgcaggc gtgcaagccg cccgccccgc caaaggctat
      721 caacgtgtag tcacgcgggt cgtggccgcg ttccacggaa atgacgcgaa tagctttttc
      781 catcactgcg ttggccaccg cgatgatgcc ttgggcgaaa gcttccacgg tgcggaatcg
      841 ctcgtcagat tgtttgacaa cattttgcat ttccgcacgc gcccgcggaa tatccagcga
      901 aaatgatccc ccgagaagtc ctgcatctcc gagatgaccg agcacggcgt gagcatcggt
      961 gacggtggga ttcattccac gcccgtaaca gaccggtcca ggcaccgcgc cggcgct
//
LOCUS       DATCBB010000731         1090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_413138, whole genome shotgun sequence.
ACCESSION   DATCBB010000731 DATCBB010000000
VERSION     DATCBB010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413138"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VII37_08150"
     CDS             complement(<1..287)
                     /locus_tag="VII37_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069746.1"
                     /translation="MIPRYTREQMGAVWRDANKYAKWLEVELAATETLAEAGDVPKEA
                     AAKIRANAKIDVARIQELESRVKHDVVAFTMAVGESIGDPAAARWFHYGMTS"
     gene            536..>1090
                     /locus_tag="VII37_08155"
     CDS             536..>1090
                     /locus_tag="VII37_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5069747.1"
                     /translation="MNESVESVRAADAIAIERTIAGDRDAFRVLVERHSATIFRLAYR
                     LTGNHHDTEEIVQEAFLRAYQKLEQFASQANFATWVYRIAANYTIDRMRQRKQEDARK
                     QAPSAEMSDDVEQDPLRQVPDAAPTPERLTQSVELRRQMQNALQSLTPAERIAFVMRH
                     CDGCGIEEIAAALKSNSGAAKNTVF"
BASE COUNT          244 a          311 c          312 g          223 t
ORIGIN      
        1 gaagtcatgc cgtagtgaaa ccagcgcgcg gcggcgggat cgccaatgga ttcgccgacg
       61 gccatggtga aggccaccac gtcatgcttt acgcgcgact ccagctcctg aatgcgcgcg
      121 acatcgattt tggcgttggc gcgaatcttg gccgcggctt ccttgggcac atcgccagct
      181 tccgcgagcg tttcggtcgc ggccagctcc acctcgagcc acttggcata tttgttggca
      241 tcgcgccaca cggcgcccat ttgctcgcgc gtgtatctag gaatcaacct aatctcctca
      301 atttccttgc ggactcgatg tgcacaaccg gtttgcggag gcggcttccg cgcgcaaatc
      361 catggtgcaa atccacggta atgatacggt atttcaaacc acgacgcgac tttcgcggtt
      421 tgccgcatca agatgttgca acggcgtgcc ttgcagtatt caaataagcg ccaactgagc
      481 caccgggccc ttggcggacc taaccgcgag cctgaaaacc tgtctaagga tacgaatgaa
      541 cgaaagcgtt gagagcgtcc gggcagccga cgccattgcc atcgagcgca caattgcggg
      601 cgatcgcgat gcttttcgcg ttctcgtgga gcgccatagc gccaccattt tcaggctggc
      661 gtaccggcta accggcaacc atcacgacac cgaggaaatt gtgcaggagg catttttgcg
      721 tgcttatcaa aaactcgaac agtttgcctc gcaagcgaat tttgccactt gggtgtatcg
      781 catcgccgcg aactacacca tcgatcgcat gcgacagcgt aaacaggaag atgcgcgcaa
      841 gcaagctccg agcgcggaga tgagcgatga cgtggagcag gatcctctac ggcaggttcc
      901 ggacgctgca ccgacaccgg agcggttgac gcaaagcgtc gagctcagaa gacagatgca
      961 aaatgcgctg caaagtttaa ctcctgcgga acgcatcgcg tttgtgatgc ggcattgtga
     1021 cggttgtggc attgaagaaa ttgccgcggc gctgaaatcc aattcgggcg cagcgaagaa
     1081 tacagttttt
//
LOCUS       DATCBB010000732         3415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_218433, whole genome shotgun sequence.
ACCESSION   DATCBB010000732 DATCBB010000000
VERSION     DATCBB010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218433"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VII37_08160"
     CDS             <1..222
                     /locus_tag="VII37_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069748.1"
                     /translation="AVVFVLFSCSNSKPTTNQIAHRNPNQNPNRIRKPTPNQKKLPGK
                     AAHKYAHNRFSTPMGLDHRSVTFSVRAVS"
     gene            359..1204
                     /gene="kdsA"
                     /locus_tag="VII37_08165"
     CDS             359..1204
                     /gene="kdsA"
                     /locus_tag="VII37_08165"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY5069749.1"
                     /translation="MSPHPPHEIALGPLRLGAANPLFLIAGPCVIESEAHACGMAEKI
                     ARIAHDCGVPYIFKASYDKANRSSLKSFRGPGLAEGLRILKKIKKDFAVPVLTDIHES
                     TQAAPVAEVCDILQIPAFLSRQTDLLVAAAKTGRIINVKKAQFLSAWDMSNVVEKITA
                     AGNEQIILTERGASFGYNNLVVDMRAFPVMQKLGYPVVYDVTHSVQLPGGQGHASGGQ
                     PEFIETLARAGVAAGVDGIFLETHDNPSAALSDGPNALPLAQLPPLLKRLKQVAELVR
                     SWSPR"
     gene            1201..2160
                     /locus_tag="VII37_08170"
     CDS             1201..2160
                     /locus_tag="VII37_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY5069750.1"
                     /translation="MSLDCARRVLRIEAQAIQDVMARLDGSFERAVDVLFACKGRVVV
                     SGMGKSGLIGRKISATLSSTGTPSFFLHPAEALHGDLGMLARGDALLAVSYGGETEEI
                     VRLLEALKRLEMSLVTLTGKTDSTLAQASDVVLDVSVKEEACSLNLAPTASTTVAMAV
                     GDALAVALLERRGFQPDDFAALHPAGRLGSKLLRVEHLMHAGEALPRVSPETMMPDVF
                     HEMSKKGLGMTTVTEADGRLAGILTDGDLRRLMEKHRGATLEMRAGDCMTRGAQTIGP
                     HVLASEALNLMEKRKITSIVVVDEVKAALGVVHLHDLWTLELM"
     gene            2200..2772
                     /locus_tag="VII37_08175"
     CDS             2200..2772
                     /locus_tag="VII37_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY5069751.1"
                     /translation="MAKAAKKIGARITAQVAKRAKQITVFLMDVDGTITAGGVTLLSL
                     ENGSTEEIKTFDAHDGQGLSLAHTAGIRTGVITGRGSAALQRRAKELKIEFVYEHQAH
                     KIAAYEEILKKTGASEAMVAYLGDDLPDITIMRRVGLAVAVGDAAPEVKRVAHHVTRA
                     IGGKGAAREVVELILKSKGIWEEMIDKARA"
     gene            2835..>3415
                     /locus_tag="VII37_08180"
     CDS             2835..>3415
                     /locus_tag="VII37_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY5069752.1"
                     /translation="MLHAWHDVSPGDDLPRDFQAVIEIPLGSNVKYELDKTSGMLRVD
                     RIIHSAVYYPANYGFIPQTYAEDDDPLDVLVLCQEAVQPLALIQARAIGLMTMVDSGA
                     SDDKVIAVATGDPEFNSYVEARDLPPHRLLVLKRFFSDYKQLEGKTVQVEDIRPAYAA
                     ITVIEKALARYKMVREKLLDDDGQGPIKFPVPK"
BASE COUNT          815 a          858 c         1024 g          718 t
ORIGIN      
        1 gccgtggtct tcgttctttt ttcgtgctcc aactccaaac caaccactaa ccaaatcgcc
       61 cacagaaacc ccaaccaaaa ccccaaccga atccgcaaac caacccccaa ccaaaaaaag
      121 ctaccaggca aggctgcaca taaatatgcc cataaccggt tctccacccc catggggctg
      181 gaccaccgat ccgtaacatt ctcggtgcgc gcagtcagct agcccagccg ttcgccaagg
      241 ttcacccccg cgaaggctgc gccgcccagg gatgaatggc gatgaatgag cttctcacgc
      301 ccttcgtgtt atcttcagtg ttccgcatga cacgaattca taaaattgcg tggagcgcat
      361 gagcccgcat ccgccgcacg aaatagcgtt gggcccgctg cggctgggcg cagcaaatcc
      421 gctcttcctg atcgctggcc cctgcgtaat cgaaagcgaa gcccacgcct gcggcatggc
      481 cgaaaaaatc gcgcgcatcg cgcacgattg cggcgtcccc tatattttta aagcttcgta
      541 cgacaaagcc aatcgctcat cgctcaaatc ttttcgcggc ccaggactcg cagaaggctt
      601 gcgcatccta aaaaaaataa aaaaagactt cgccgtccca gtgctgaccg atattcatga
      661 aagcacgcaa gccgcgccag tcgcagaagt ctgcgacatt ttgcaaatcc ccgccttctt
      721 gtcgagacaa accgatctgc tggtcgcggc agcaaaaact ggccgcatca tcaacgtaaa
      781 gaaagcacaa tttctttcgg cctgggatat gagcaacgta gtagaaaaaa taaccgccgc
      841 aggaaatgaa caaatcattt tgaccgagcg cggcgcctcc ttcggctaca acaatttagt
      901 cgtggatatg cgcgcattcc cggtgatgca aaaactcggc tacccagtag tctacgacgt
      961 gactcattca gtgcagctac ccggcggcca agggcacgcc agcggcggcc aaccggaatt
     1021 tatcgagaca ctcgcgcgcg cgggagtagc cgcaggcgta gatggaattt ttctagagac
     1081 gcacgacaat cccagcgccg cgctttccga tgggccaaat gcgctgccgc tcgcgcagtt
     1141 gcctccgctg ctgaagcgat tgaagcaagt tgcagaattg gtacgaagct ggagtccacg
     1201 atgagtctcg attgcgcgcg ccgtgttctg cgcatcgagg cgcaagccat tcaagatgta
     1261 atggcgcgcc tggatggcag cttcgagcgc gccgtggatg tgttgtttgc gtgcaagggc
     1321 cgcgtagtgg tcagcggcat gggcaaatcc ggtttgattg ggcgtaaaat ttcggcgacg
     1381 ttgtcgagca ccggcacgcc gtcgtttttt ctacatcccg cggaagcgct gcacggcgat
     1441 ttgggaatgc tggcacgcgg cgacgcgctg ctggcggttt cttacggcgg agagacagaa
     1501 gaaattgtgc ggctgctcga agcactgaag cgcctcgaga tgtcgctggt caccttaacg
     1561 ggaaaaacgg actcgacact agcacaagcg agcgatgtag tgttggacgt cagcgtgaag
     1621 gaggaagcgt gctctttgaa tctcgcgccc acggcgagta ctacagttgc catggccgtg
     1681 ggcgatgcgc tagccgtggc gcttttggag cggcgcggat ttcagcctga tgattttgcg
     1741 gcgctgcatc ccgcgggccg attggggtca aaactgcttc gtgtggaaca tttgatgcac
     1801 gcgggcgaag cgttgccgcg cgtatctccc gagacgatga tgcccgatgt gtttcacgag
     1861 atgagtaaga agggcctggg catgacaacc gtgacggagg cggacggaag attagcggga
     1921 attttgaccg atggagattt gcggcgtttg atggagaagc atcgcggcgc cacgctggaa
     1981 atgcgcgcgg gagactgcat gacgcgcggc gcgcaaacga ttggcccgca tgtgttggcg
     2041 agcgaggctt tgaatttgat ggagaagcgc aagattactt cgattgtggt ggtggatgaa
     2101 gtaaaagcag ccttgggagt agtgcatttg catgatttgt ggactttgga attgatgtaa
     2161 acgcgtacag cgggaaaacg atctgcggag gaattttaat tggctaaagc ggcgaagaaa
     2221 attggcgcgc ggattacagc gcaggttgca aagcgagcga aacagattac cgtgtttttg
     2281 atggacgtag atggcacgat taccgctggc ggggttacgt tactttcgtt ggagaatgga
     2341 tccaccgaag aaatcaaaac gttcgacgcc catgatgggc aggggttgag tctggcgcac
     2401 acggcgggaa ttcgtaccgg cgtgattacc gggcgaggga gtgccgcatt gcagcgacgc
     2461 gcgaaagaac tgaaaatcga atttgtctat gaacatcaag cgcacaagat tgcagcgtat
     2521 gaagaaattt tgaagaagac cggcgcctct gaagcgatgg tggcttatct cggcgacgat
     2581 ttgccggata ttacgatcat gcggcgagtt ggcttggccg tggcggtggg agacgcagct
     2641 ccggaagtaa aacgcgtggc gcatcatgtg acgcgcgcga tcggcgggaa gggtgcggcg
     2701 cgtgaagtag tggagttaat tttgaagagc aaaggaattt gggaagagat gatcgataag
     2761 gcgcgggcgt gatccgcggg agaattatct gtttgcgttt ttggattcgg ccacagtaag
     2821 gaaagaggga ttttatgttg cacgcatggc atgacgtttc acctggcgat gatcttcccc
     2881 gggattttca agcggtaatc gagataccgc tgggatccaa tgtgaagtac gagttggata
     2941 agaccagcgg catgctgcgc gtggaccgca tcattcattc ggcagtctat tatccagcca
     3001 actacggttt cattccgcaa acttacgcgg aagatgatga tccactggac gtgctggtgc
     3061 tatgccagga agcggttcag cccctcgcgc tgattcaagc gcgcgccatc ggattgatga
     3121 ccatggtgga ctcaggtgcg agtgacgaca aagtgatcgc agtggctact ggtgatccgg
     3181 agttcaatag ttacgtggaa gcgcgggact tgccacctca ccgtttgctg gtgcttaaga
     3241 gatttttttc agattacaaa caactggaag gcaaaaccgt gcaggtggag gatattcgtc
     3301 cggcctatgc ggcaattacg gtgattgaaa aagcattggc gcgatataaa atggttcgcg
     3361 agaaattgct ggacgatgat gggcaggggc cgatcaagtt tcctgtgccg aaatt
//
LOCUS       DATCBB010000733         1150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_249840, whole genome shotgun sequence.
ACCESSION   DATCBB010000733 DATCBB010000000
VERSION     DATCBB010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249840"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VII37_08185"
     CDS             complement(<1..1130)
                     /locus_tag="VII37_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069753.1"
                     /translation="MLAGSGLVGITNLVSNVVTARLLGPVGFAHATAVYTILMLVSCI
                     TLAFQVVSAKYIARNPLVEDRVSVFASLHRRAWIAGISIGALLFLFERPLTNYLNLPD
                     PVLISLLALGTAFYIPLGVRRGYIQGVHAFRALAINFMLEGLVRLGGLSLLIGLGMGV
                     KGAVLSSSLAVVVCYFAAIPSPGLVSLRRHGVPISFREGLQAIVFFSGQTVINNIDIV
                     LVKHFFPPAEAGFYAAVALVGRLVNMCAWSVVNTMFPVSAGAGEDEQEGRPVLMTSLL
                     LVFGILSVLVFGLWLVPSFLWKILFGAQFGLGNYGALAPLLILYAVTTGVYSLSSVII
                     TYEMSRKIANTSWLQLVFSVALAVGVYTLHQTLRQVILVQLV"
BASE COUNT          332 a          334 c          309 g          175 t
ORIGIN      
        1 aaaaccagct ggacgagaat tacctggcgc agagtttgat gcagcgtgta cacgcccacc
       61 gcaagagcca cgctgaatac caattgcagc cagctggtat tcgcgatctt acgcgacatt
      121 tcgtaagtga tgatcaccga actcagcgaa taaacgcccg tggtgacggc gtagaggatc
      181 agcagcggcg ccagcgctcc gtagttgccg agtccaaatt gagcgccaaa aagaatcttc
      241 cacagaaaac ttggaaccag ccacaggccg aataccagca cgctaagaat gccgaaaaca
      301 aggagcaagg aagtcatcag caccgggcgc ccttcctgct catcttcgcc cgcgcccgcc
      361 gaaaccggaa acatggtatt gaccacggac cacgcacaca tgttaaccaa ccgtcccaca
      421 agagccacgg ccgcatagaa tcccgcttcc gccggcggaa aaaaatgttt taccagaacg
      481 atgtcaatat tgttgatgac cgtctggccg gaaaagaaaa cgatcgcctg caagccttcg
      541 cggaaggaaa tcggaactcc atgacgacgg agagaaacga gcccggggct cgggatcgcg
      601 gcaaaataac aaaccacaac tgcgagactg ctggaaagga cggccccttt cacgcccatg
      661 cctagtccga taagaaggga aagaccgccg agccgaacca gcccttccag catgaaattg
      721 atagccagag cgcgaaacgc gtgaactccc tggatatagc cgcgccgtac tcccaaagga
      781 atatagaatg cggtgccgag cgcgagtaat gaaatcagaa ccggatcggg caaattcaga
      841 tagttggtca gcggccgctc gaacagaaaa agcagtgcgc caattgaaat tcccgcaatc
      901 caagcgcggc ggtggaggct ggcaaagacg ctgacgcgat cttcgaccag cggattccgg
      961 gcaatgtact ttgccgacac gacctggaac gccaaggtga tgcacgacac cagcatcaaa
     1021 atggtgtaaa ccgcggtggc gtgtgcgaat cccaccggac ccagcagccg ggccgttacg
     1081 acattggaga ctagattcgt aatcccgacg agtcccgaac ccgcgagcaa cgtaagcgtg
     1141 ccgccaagaa
//
LOCUS       DATCBB010000734         1420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_218480, whole genome shotgun sequence.
ACCESSION   DATCBB010000734 DATCBB010000000
VERSION     DATCBB010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218480"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VII37_08190"
     CDS             complement(<1..472)
                     /locus_tag="VII37_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069754.1"
                     /translation="MSFQDLLKDTLSTLWAHKRRTLLTMFGIAWGIISITVMVAAGEG
                     LGKGIQANQETFGKDVMIVFAGRTSMQAGGTRSGRAVHWGEDDYLQVAAESPACKYVM
                     PELGNNVQVHSLYNSGSISTVGSMPEFTEIRSITTAEGRFYNHEDNEEARNVAFL"
     gene            complement(492..>1420)
                     /locus_tag="VII37_08195"
     CDS             complement(492..>1420)
                     /locus_tag="VII37_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5069755.1"
                     /translation="ILAELDKIQLEAAQRAAKANLQAAEAAHHSSMAALERNKVDAEG
                     PDVPFLKLTEERAEHEFNEGVVSKSVVEDAEKNYRMALNKQASAQAALAVAHADIAKA
                     EAQVAQSRAALDNAEEDLRNSTIVSPIDGLVLSRDVNVGDAVSSILILGSQATPIMTL
                     GDVSEVYVQGKVDEADIGKVYLNQPARIVVESFKDKKFNGKVTKISPLGKEKDNVTTF
                     EVRVSIENSTLELKANMSANAEIMLEEKKNVLMVPEAALIYDKERKAQAELPDAKGPN
                     GKKKVHVKLGISNGVKTEILEGLNEKQQVILQ"
BASE COUNT          271 a          437 c          323 g          389 t
ORIGIN      
        1 ccaaaaatgc cacgttgcgc gcttcctcat tatcttcgtg attgtaaaac cgcccttccg
       61 ccgtggtgat actgcggatc tccgtaaact ccggcatcga gcccaccgtc gaaatacttc
      121 cgctgttgta cagactgtgg acctgcacat tgttccccag ctccggcatc acgtatttgc
      181 acgcaggcga ttccgccgcc acctgcaggt aatcgtcttc gccccaatgc accgcgcggc
      241 cagagcgcgt ccctccggct tgcatactgg tgcgcccggc aaagacgatc atcacgtctt
      301 ttccaaatgt ctcctgattt gcctggattc ccttgcctag accttcaccc gcggccacca
      361 tcacggtgat ggaaataatt ccccacgcga tgccgaacat ggtcagcagc gttcttcgtt
      421 tgtgcgccca caaagtgctg agcgtatcct tcagcaaatc ctggaatgac aaagcgccgc
      481 cttattcgta gctattgcag gattacctgc tgcttctcat ttaatccttc aagaatttcc
      541 gttttcacgc cgttggagat tcccaatttc acatgtactt ttttctttcc gttggggcct
      601 ttggcatctg gaagctccgc ctgcgctttg cgttctttgt cataaatcag cgcggcttct
      661 ggaaccatca gtacgttttt cttctcttcc aacataattt ctgcgttggc gctcatattg
      721 gcttttagtt ccagcgtgga attctcaatg gagacgcgaa cctcaaacgt ggtgacgtta
      781 tctttctcct tgcctaaagg agaaatcttc gtcaccttgc cgttgaattt cttgtctttg
      841 aaggattcca ctacgatgcg cgcgggctga ttcaaataaa ctttgccaat atccgcttca
      901 tccactttgc cctgcacgta gacttcgctg acatcgccga gagtcatgat cggcgtggcc
      961 tgcgaaccga gaattaagat cgagctcacc gcgtcgccta cgtttacgtc gcgtgaaaga
     1021 accaagccgt cgatggggct gacaatcgtg gagttgcgca agtcctcttc cgcgttatcc
     1081 agcgccgcgc gtgattgcgc tacttgcgct tccgccttcg cgatgtccgc atgcgccacc
     1141 gccagtgccg cctgagcgct agcttgctta ttcaaagcca tgcgataatt tttttctgca
     1201 tcctcgacca ccgactttga cactacgcct tcgttgaatt cgtgttccgc gcgctcctca
     1261 gtaagcttca gaaacggcac atccggccct tcggcatcca ctttattgcg ctccagcgcc
     1321 gccatcgagg aatggtgcgc cgcttcagcc gcctgtaaat tcgctttggc cgctcgttgc
     1381 gccgcttcga gttggatttt gtccaactcc gccagaattt
//
LOCUS       DATCBB010000735         1569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_379872, whole genome shotgun sequence.
ACCESSION   DATCBB010000735 DATCBB010000000
VERSION     DATCBB010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379872"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VII37_08200"
     CDS             complement(<1..581)
                     /locus_tag="VII37_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY5069756.1"
                     /translation="MTNSPTIDLVGVGLNAIDTLIPLPYYPELGSKVEFRSANILPGG
                     QVASAVIACQQWGLNTRYVGKVGEDRFADIHRAEFAKAGVEAHLFTAPGCASQQAFIL
                     VDDAGERTVLWKRDNNLTLRAEELKQEWIVGARALHVDGHDTAAAIQAATWAKASGLP
                     VIADLDEVYPGVQGLLGSIDYLIASRDIPGRLTG"
     gene            complement(585..>1569)
                     /locus_tag="VII37_08205"
     CDS             complement(585..>1569)
                     /locus_tag="VII37_08205"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEY5069757.1"
                     /translation="LTIFAIRDIGLSIAFPLWNSNSLLGIFWGFVFFCELRQAGWRRW
                     TGVLGGALIMCVGATLLAIASSTQAPAGHSMRGVWAALGAGILWGTMYIPYRKAYLSG
                     MNPLSFITFFTFGELGMMTTLTVSYLGFAVLWRELVSARAVLFWLMAGGFIWVIGDLF
                     QQYAAKYLGISRGIPLSNSNQLWGLLWGIFVFGELHGSGASTYMQVIGGSLLMALGVA
                     AIAFSSTTGAEQVQWAAATQRECQRYGMDSEFAAARMQGEDLAEGGRLVREPLDWIVV
                     AVATGVFIFFATLAHAPSLPFNAPIAVLLSAAIVILLAICGVRLWRTTRFN"
BASE COUNT          404 a          494 c          370 g          301 t
ORIGIN      
        1 cctgtgagac gccccggtat atctctgctt gcaatcaggt agtcgatgct acccagcaaa
       61 ccctgcacac caggataaac ttcatcgaga tcggcgataa ccgggaggcc tgaggcctta
      121 gcccacgtgg ctgcttgtat cgccgccgcc gtgtcgtgac cgtcgacatg caaagcccgt
      181 gctccgacaa tccactcttg tttcagttcc tccgctcgca gcgttaaatt attgtcgcgc
      241 ttccacagca cggtgcgctc accggcatcg tcgaccaaaa taaacgcctg ctggctcgcg
      301 caccctggag cggtaaatag atgtgcttcg acaccggcct tcgcaaattc cgcgcggtga
      361 atgtccgcaa agcgatcttc gccaactttt ccaacgtacc ttgtgtttaa tccccattgt
      421 tggcaggcaa tgactgcgct cgcgacctgg ccaccaggca aaatatttgc tgagcgaaac
      481 tctaccttgg accccaactc cgggtaatag ggcaacggaa ttaacgtgtc gatcgcgttt
      541 agtccgacgc cgacgaggtc aattgtgggt gagttggtca tgaattaatt gaagcgagtc
      601 gttcgccaca gcctgacacc gcaaatagct aggaggatga caattgctgc gctcaacaac
      661 accgcgatcg gcgcgttaaa aggcaaactt ggcgcgtgcg caagagtcgc aaaaaatatg
      721 aagacgcccg tggccactgc aaccacaatc cagtcgaggg gctcgcgtac aagtctgccc
      781 ccttccgcca aatcttcacc ctgcattcgc gctgctgcga attcgctatc cataccatag
      841 cgctggcatt cccgttgcgt agccgccgcc cactgtactt gctccgcgcc agtcgtcgac
      901 gaaaacgcaa tcgccgcgac gcccaacgcc atcagcaacg aaccgccgat gacttgcata
      961 tacgtagatg caccacttcc atgtaattct ccaaaaacaa aaatccccca caacaatccc
     1021 cacagctgat tcgagttcga tagcggaatg ccccggctta ttcccaaata cttcgcagca
     1081 tattgctgga atagatcgcc gatcacccaa atgaatcctc cggccatcag ccagaaaagc
     1141 accgcacgcg cgctgaccaa ctcacgccaa agaaccgcga aaccaagata actcaccgtg
     1201 agcgttgtca tcattcccag ctcgccaaag gtgaaaaagg taatgaatga aagtggattc
     1261 attcccgaga gataggcctt tcgataagga atgtacatcg ttccccagag tattccggcc
     1321 cccagagcgg cccacacgcc acgcatggaa tggccggccg gagcttgcgt tgaagaagca
     1381 atcgccagca gcgttgcgcc tacacacatg atcagcgcgc cgccaagcac cccggtccaa
     1441 cggcgccagc ccgcttgacg caactcgcaa aagaaaacaa agccccaaaa tatgcccaac
     1501 aggctgttcg aattccacaa aggaaacgcg atgctcagcc cgatgtcgcg aatagcaaaa
     1561 attgtcagc
//
LOCUS       DATCBB010000736         1358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_88076, whole genome shotgun sequence.
ACCESSION   DATCBB010000736 DATCBB010000000
VERSION     DATCBB010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88076"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1283
                     /locus_tag="VII37_08210"
     CDS             213..1283
                     /locus_tag="VII37_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY5069758.1"
                     /translation="MPRHARPLECAAETRAELVAISRSRAGEVRMVERARIVLACLEG
                     KEIQQVAIETGASIPTVGKWRKRFGEKGLSGLKDLPRSGKPPTFGAAFRDRVLALLEQ
                     APPSGQSQWDGPAVAERLNASVHAVWRVLRREGIYLQRLRTWCVSTDPEFATKAAEVV
                     GLYLNPPLNALVISVDEKPSIQAIQRPSGYVETDSGAIVRAMKSTYKRNGTLNLFAAL
                     EIASGQVHAQTTERKKREDFRRFLDSVILELPADREIHVILDNYSTHKRNTDWLEKYQ
                     GRVQFHFTPTSASWLNQVEIWFGLLTRKALRGASFASKDNLRIAIEAFVAKTNEHPKP
                     FHWRKREVKGSQLRNTIINLCN"
BASE COUNT          306 a          359 c          395 g          298 t
ORIGIN      
        1 agcgtcttgc gccatgccgc ttgttggctg tcgttgattt cgcccaaata caaaacttgt
       61 ctctgcgcga cttttcccgg atctcagacg ccggctctct accactgtgt agtagcggtg
      121 ctctttccca tcttttgcgg gatcttgaac cagctatttg caagctagtg cttagttgcg
      181 atagtacatt attgtattaa aatgtttggg agatgcccag acatgctcgt ccgttagaat
      241 gcgcggcgga aacccgcgcc gagttagttg cgatcagccg aagccgcgcg ggcgaagtcc
      301 ggatggtgga gcgggcccgg atcgtacttg cgtgtttgga aggcaaggaa attcagcaag
      361 tagcaatcga gaccggcgcc tctattccta cggtcggcaa gtggcgcaaa cgattcgggg
      421 agaaaggact gtccggcttg aaggatctgc ctagatctgg caagccgcca acgtttggcg
      481 cggcttttcg tgatcgagtg ctggctttgt tggagcaagc gccgccgtca gggcagtcgc
      541 aatgggatgg tccggcggtg gcggagcggt tgaacgccag cgttcatgcg gtatggagag
      601 tgcttcgccg cgaaggaatt tatctgcaac gtctgcgaac ttggtgtgtg agcaccgacc
      661 cggagttcgc cacgaaggcg gcggaggttg tgggcttgta cttgaatccg ccgctgaacg
      721 ccttggtgat tagtgttgac gagaagccca gcatccaagc gatccagcgg ccttccggct
      781 acgtcgaaac cgacagcgga gccattgtcc gggctatgaa aagcacgtac aaacgcaacg
      841 gcacgctgaa tcttttcgcc gcgctggaga tcgccagcgg acaggttcac gcgcaaacga
      901 cggaacggaa gaagcgtgaa gacttccgcc gctttctgga ttcggtgatt ctggagttgc
      961 ccgccgaccg ggagatccat gtaattctgg acaactacag cacgcacaag cggaacacgg
     1021 actggctgga aaagtatcag ggccgcgtcc aattccactt caccccgacc tcagccagct
     1081 ggctcaatca agtcgaaatc tggttcggtc ttttgacccg caaagcactg cgcggggcca
     1141 gtttcgccag caaagacaac cttcgcatcg ccattgaagc gttcgtcgcc aaaaccaatg
     1201 agcaccccaa gccattccac tggcgtaagc gcgaagtcaa gggtagtcag ttgcggaata
     1261 cgattattaa cttatgcaac taagcactag cgacacatcg ctctttttag attgatatgc
     1321 cataagaact gtgatagtgt cccaaataag ttcggagg
//
LOCUS       DATCBB010000737         1017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_218615, whole genome shotgun sequence.
ACCESSION   DATCBB010000737 DATCBB010000000
VERSION     DATCBB010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218615"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VII37_08215"
     CDS             <1..927
                     /locus_tag="VII37_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5069759.1"
                     /translation="RISKRNVDLEEHIAAPGENAPLFWLDVVTSNYFRVMGISTLAGR
                     EFNDADEAGSPVAMVTASTARRYWPGENAVGKHIRLLKEEGWRTVVGVIPDVRAFDIQ
                     SGEPEWIEGTAYVPYNSSATLEDGRVPAEMTIAVRTKMDESQAEGTLRRLVSGLNPEV
                     PISEMKTMQEVVSEAVATPASTTSLFATFASLALVLGMIGIYGVLTFLVSKRTREIGI
                     RMALGARKQDVLWLVIREGAKLSLTGIAIGLAGAVVVTRLLASELYGVSPMDAVTYFS
                     VTAAMGVVTMLACYVPTRRAMRVDPLTALRQE"
BASE COUNT          234 a          226 c          343 g          214 t
ORIGIN      
        1 agaatcagca aacgcaacgt ggacctggaa gagcacatcg ctgctcccgg cgaaaacgcg
       61 ccgctgttct ggctggatgt ggtcacttcc aattattttc gtgtgatggg cattagcact
      121 ttagcgggaa gagaattcaa cgacgcggac gaggccggaa gtccagtggc gatggtgacc
      181 gcgagcacgg cgcgacgcta ctggccgggc gaaaacgcag ttggtaagca tatccggctg
      241 cttaaagagg aaggctggcg tacggtggtg ggagtgattc cggacgtgcg cgcttttgat
      301 attcagagcg gcgaaccgga atggatcgaa gggaccgcgt atgttccgta caattccagc
      361 gcgacgctcg aagatggcag ggttccggcg gaaatgacta tcgcggtgcg gacgaagatg
      421 gatgagtcgc aagcggaggg aacgctgcgg cgactggtta gcgggctgaa tccggaagta
      481 ccgataagcg aaatgaaaac gatgcaagag gtggtgtcag aggcggtagc aacgccagct
      541 tccaccactt ccctgttcgc tacgtttgcg tcactggcat tggtgctggg gatgatcgga
      601 atctatggag tgctgacgtt tctggtttcg aagcgcacgc gggaaatcgg tattcgcatg
      661 gcgctggggg ccaggaagca ggatgtgttg tggctggtga tacgggaagg cgcgaaactt
      721 tcgcttacgg gtatcgcaat aggcttggcc ggggccgttg tggttacgcg attgttagca
      781 agcgaactgt acggtgtgag cccgatggat gcggtaactt acttcagcgt gactgcagcg
      841 atgggggtgg taaccatgtt ggcttgctat gtgcccacga gacgtgcgat gcgcgttgac
      901 ccactaaccg cgttgcggca agagtgatga aaagggcaag ctagagcggc tgaagtggcc
      961 agcttggcca gctaatttaa taagtctaat gtgttcatac acttacatta aactaaa
//
LOCUS       DATCBB010000738         1105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_413433, whole genome shotgun sequence.
ACCESSION   DATCBB010000738 DATCBB010000000
VERSION     DATCBB010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413433"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..862)
                     /locus_tag="VII37_08220"
     CDS             complement(26..862)
                     /locus_tag="VII37_08220"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5069760.1"
                     /translation="MDRVCDTSESALAGKRVVITRAARQSVELVEKLEKLGAMPILLP
                     LVAFSAPEDYGPLDAALDRLEQFDWIIFTSENAVRAVVKRAGVRGNLRNVAGRRSRAA
                     AVGPTTAVAAERAGFFVDYQAQTHSGAALANELGEKLRGQTIFLPRSDRANPDLPQLL
                     RNYGAEVTEVIAYRTVTPVNLDEEKIAAIVNGQVEAILFFSPTAVEHFVGIVGKEKLR
                     NLQSRLAISAVGPITANALRQAGVDILLVAEDTTADAVIVALERHFASTQKASAAGVV
                     HE"
     gene            complement(855..>1105)
                     /locus_tag="VII37_08225"
     CDS             complement(855..>1105)
                     /locus_tag="VII37_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069761.1"
                     /translation="TERALLAALQGGCQVPIGAWARIERGEMVLEACVTSVDGQQHVK
                     QRLTGAADQGPALGEQMARLLIESGAQEILEAVGRQRG"
BASE COUNT          221 a          352 c          281 g          251 t
ORIGIN      
        1 cgccgccctc gatgtgtcgg gaagctcatt cgtgaactac tcccgcagcc gaggctttct
       61 gtgtgcttgc gaaatgtctc tcaagcgcca cgatgaccgc gtccgcagtg gtatcctcgg
      121 ccaccagcaa aatatccacg cccgcttggc gcaacgcatt cgcggttatg ggtccgacag
      181 cagaaatcgc caaccggctt tgcaaattgc ggagcttctc tttacccacg attccaacga
      241 agtgctccac ggcggtggga ctgaaaaata aaattgcctc aacttgacca ttcacaattg
      301 cagcaatttt ttcttcgtcg agattaaccg gcgtaactgt ccggtacgcg ataacctcgg
      361 tcacttccgc accatagttt ctcaaaagct gtggcagatc cggattggcg cggtcgcttc
      421 gcggtaaaaa tatcgtttgg ccgcgcagtt tctctcccag ttcattggcg agtgctgctc
      481 cactgtgggt ttgcgcctgg taatccacaa aaaaacctgc gcgttccgct gcgactgccg
      541 tggtcggtcc aactgctgca gcgcgcgaac gtcttcccgc cacattgcgc aaattgccgc
      601 gaactcccgc gcgcttaact accgcgcgca ctgcattttc gctggtgaag ataatccaat
      661 cgaattgctc caatcgatcc aaagctgcgt ccaacgggcc gtagtcctct ggagccgaga
      721 aggccacgag cggcaacaaa atcggcatgg cgcccaactt ctcaagtttc tctaccagtt
      781 caacactttg gcgcgcagcg cgagtgatca ctacgcgttt gcctgctaag gcggattcag
      841 aagtgtcgca gactctatcc acgctgccgt cctactgctt cgagaatttc ttgcgcgcca
      901 ctttctatca gcagccgggc catctgttct ccgagcgcgg ggccttggtc agccgcgcct
      961 gtcagacgct gttttacatg ttgctggccg tccactgagg tcacgcaggc ttccagcacc
     1021 atttcgccgc gctctatccg ggcccatgct ccgatgggca cctgacagcc accctgtagc
     1081 gcggccagca gcgcgcgctc agtaa
//
LOCUS       DATCBB010000739         1287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_121616, whole genome shotgun sequence.
ACCESSION   DATCBB010000739 DATCBB010000000
VERSION     DATCBB010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121616"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1287
                     /locus_tag="VII37_08230"
     CDS             <1..>1287
                     /locus_tag="VII37_08230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5069762.1"
                     /translation="ASAQDAAQTPAPSQKQTPPVGGAPKPFNVPAHETYALPNGMRVT
                     LVPYGNIPKVAVSLAIRAGNLNEPQDVLGVADITGELLKEGTKTLSAQALAEAAARMG
                     SSLSVTVGADESAIDLDVLSEYGGRAVSLVSDVAMHPLLPESELARLKANLLRQVLVA
                     KSRPGQIALARFRKLLYGDHPYGEILPTEASLNKLTIEDARKFYAGNYGAERAHLYVA
                     GKFDVAEVKKAIAAGFSDWSKGPAPVLNVPHPKTERLLDVTDRPGAPQSTIYVGLPVI
                     DPTNPDNIPLTVTNALLGGSFGSLITSNIREQKGYTYSPTSQLSRRYHDAYWAEIADV
                     TTAATGPSLKEIFGEIDRLQKEAPAAAELKGIQSYISGLFVIQNSTRQALIGQLRNVD
                     LQGLGEDYLKNYVQRVNAVTPDDVQHMTAKYIKPEQ"
BASE COUNT          307 a          357 c          379 g          244 t
ORIGIN      
        1 tgcgtccgcg caggatgcgg cgcaaacacc agcgccttcg caaaaacaaa cgcccccagt
       61 gggcggcgcg ccaaaaccgt tcaatgttcc cgcacatgag acctatgcgc tgccaaacgg
      121 catgcgcgtg acgctggtgc cgtacggaaa tattcccaag gtggcggtca gcctggcgat
      181 ccgcgcgggc aacctgaacg aaccgcaaga tgtgctgggc gttgcggaca tcaccggcga
      241 attacttaag gaaggcacca aaacgctgag cgcccaggca ctcgcggaag ccgcggcaag
      301 aatgggcagc agcttgagcg tcacggtggg cgcggatgag agcgcgatcg acttggatgt
      361 actgtcggaa tacggcggac gcgcggtaag tttggtgtcc gatgtggcga tgcatccgtt
      421 gctgccggaa tcggaattgg cgcgcctgaa ggctaatctg ctgcggcaag tattagtcgc
      481 aaaatctcgc cccggtcaga tcgcattggc gcgcttccgc aaattgctct acggcgacca
      541 cccttatggg gaaattttgc ccacggaagc cagcctgaac aaactaacca tcgaagatgc
      601 gcgaaagttt tatgccggca attatggcgc ggagcgtgcc catctttacg ttgccggaaa
      661 attcgatgta gcagaagtga agaaagccat tgccgcgggt ttctcggatt ggagcaaagg
      721 tccagctccc gttttgaatg ttccgcatcc caaaacggag cggctactgg atgtgacgga
      781 ccgccccggc gcgccgcaat cgacgattta cgtgggcctg ccggttatcg atcccacgaa
      841 cccagacaat atcccgctga cggtgacgaa cgcgctcctg ggcggttcgt ttggctcgct
      901 gatcacttcg aatattcgcg agcagaaggg ctacacctac tcacctacca gccagctttc
      961 gcggcgatat catgatgcgt actgggcgga aattgccgac gtgaccaccg cggcgactgg
     1021 gccttcactc aaggaaattt tcggcgaaat cgatcggctg caaaaggaag cgccggccgc
     1081 agcggaattg aaaggcattc agagttatat atccgggctt ttcgtgattc agaactccac
     1141 gcggcaagct cttatcgggc aattgcgcaa tgtggattta caggggctcg gcgaagacta
     1201 cctgaaaaat tatgtgcaaa gagtgaatgc cgttacgcca gacgacgtgc aacacatgac
     1261 cgcgaaatac atcaagccgg aacagat
//
LOCUS       DATCBB010000740         1154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_56522, whole genome shotgun sequence.
ACCESSION   DATCBB010000740 DATCBB010000000
VERSION     DATCBB010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56522"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1154
                     /locus_tag="VII37_08235"
     CDS             <1..>1154
                     /locus_tag="VII37_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY5069763.1"
                     /translation="PETLGSGCAFLDYDNDGWQDILLVNGMDWPGHKRQKSTLRLYRN
                     NRNGSFTDVTRAAGLDIEMYGMGVAIGDYNNDGFPDIFISCVGQSHLFRNNGDGKFTD
                     VTRAAGLLSHEGFSTSALWVDYDRDGLLDLFVCNYVRWSPEHDVFCSLDGTHKSYCTP
                     EAYRGSTCWLFHNRGDGTFEDVTATSGIFDSSSKSLGVAMFDYDQDGWPDLLVANDTQ
                     PNKLYRNLRNGKFKDVAVEAGLAFSSEGKARAGMGVDTGDFDNSGRPGVAITNFDNEM
                     IGLYRAISATAYDDISQVSGIGGPSKSTLGFGCAFLDVNLDGSLDLAVANGHIDETVR
                     NIRGNVGYAQPPQLFLNGGNGNFRDVAGEVGGGFEKSRVGRGLAYGDFDG"
BASE COUNT          250 a          294 c          339 g          271 t
ORIGIN      
        1 cccgaaacgt tgggctctgg ttgcgcattt ctcgactacg acaacgacgg ctggcaagac
       61 attctgctcg tcaatggaat ggattggccc ggccacaagc gccagaaatc gactttgcgt
      121 ttgtaccgca acaatcgcaa cggatctttc actgacgtca cccgcgccgc cggcctggat
      181 atcgaaatgt atggcatggg agtcgccatc ggcgattaca acaacgatgg ctttccggat
      241 atttttatca gctgcgtcgg gcagagtcat ctctttcgca acaacggtga cggcaaattc
      301 acggacgtga cgcgcgcggc cgggcttttg agccacgagg gctttagtac ttccgcgctg
      361 tgggtcgatt acgaccgcga tggtttgctg gacttatttg tatgtaacta tgtgcgctgg
      421 tcgccggaac acgatgtgtt ttgcagcctc gatggcacgc acaaatcgta ttgcacaccg
      481 gaagcatatc gcggcagcac ttgctggctc tttcacaatc gtggagacgg cacgttcgaa
      541 gacgtgaccg cgacgagtgg aatttttgac agcagctcaa aatctctcgg cgtggccatg
      601 tttgattacg accaggatgg ctggcccgat ctgctggtag ctaacgatac gcagccaaat
      661 aagttgtacc gcaatttgcg caacgggaaa tttaaggatg tcgcggtcga agcggggctg
      721 gccttcagca gcgaaggcaa ggcccgcgcc ggaatgggcg tggatactgg cgatttcgac
      781 aattccgggc gccccggtgt ggctatcacg aattttgata acgaaatgat tggtttgtat
      841 cgcgccattt ctgccaccgc ctacgatgat atttcacagg taagcgggat cggcggacct
      901 tctaaaagta cgctcggctt tggctgtgct ttcctggatg tgaatctcga tggctcgctt
      961 gatctcgcgg tagccaacgg tcacatcgac gaaactgttc gcaatattcg cggcaatgtc
     1021 ggttacgcgc agcctccaca gctttttctg aacggcggaa acggaaactt tcgagatgtc
     1081 gccggcgaag taggcggcgg attcgagaaa tcaagagtgg gccgtggctt ggcttacggg
     1141 gattttgacg ggga
//
LOCUS       DATCBB010000741         1110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_380063, whole genome shotgun sequence.
ACCESSION   DATCBB010000741 DATCBB010000000
VERSION     DATCBB010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380063"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VII37_08240"
     CDS             complement(<1..662)
                     /locus_tag="VII37_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEY5069764.1"
                     /translation="MTDTETHAQHAGLAHQFEGLEQQQEADALGIWIFLVTEIMFFGG
                     LFAAYAIYRWLYFAAFEGGSHILDVRLGAANTIVLLGSSLTMALSVRSAQTGNRRALI
                     LFLIATMILGGIFLGVKGYEYHQKFVEHVVPSLDWAPEGQTLARLAPGGLGHAQIYFF
                     FYFAMTGLHALHMIIGLGLLLWLVLRARKNSFTPHYFAPVEVVGLYWHFVDIVWIFLF
                     PLL"
     gene            complement(655..>1110)
                     /locus_tag="VII37_08245"
     CDS             complement(655..>1110)
                     /locus_tag="VII37_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5069765.1"
                     /translation="HFHYVMVGGMVMAYLGGVHFWFPKITGRLYPEGWAKFAALVVFV
                     GFNLTFFPQFILGYLGMPRRYAFYPAEFQVLNVMSSAGATVLGVGYLIPLIYLIWSMR
                     YGPIAEPNPWGAKGLEWETASPPPTENFAAVPVVTEEAYAYAPEEAAGD"
BASE COUNT          276 a          328 c          318 g          188 t
ORIGIN      
        1 agcaacggaa acaggaaaat ccagacgata tcgacgaaat gccagtacag gcccaccacc
       61 tcgaccggcg cgaagtaatg aggcgtgaag ctgttcttcc gcgcgcgaag gaccagccag
      121 agtaacaggc ccaatccgat aatcatgtgc agggcatgca ggccggtcat cgcgaaatag
      181 aaaaagaaat aaatctgcgc gtgaccgagg ccgccgggcg cgaggcgcgc cagtgtttgt
      241 ccttcgggcg cccaatccag gctgggaacg acgtgctcca cgaatttttg gtggtactcg
      301 taccctttga cccccagaaa gattccgccg agaatcatcg tggcgatcaa gaagaggatg
      361 agagcacggc gattgccggt ctgcgcggag cgcaccgaca gcgccatggt gagactgctg
      421 ccgagcagga caatggtatt cgcggcgccg aggcgaacat ccagaatgtg gctgccgccc
      481 tcaaatgccg caaagtagag ccaacgataa atggcgtagg ccgcgaaaag gccaccaaaa
      541 aacatgattt ccgtgaccag gaagatccag attccgaggg catcggcctc ctgctgctgt
      601 tccagcccct caaactggtg tgccagtccg gcgtgttgtg cgtgcgtttc ggtgtcagtc
      661 accggcggcc tcctccggtg catatgcgta ggcttcctcg gttaccactg gaactgccgc
      721 gaagttttcc gtaggcgggg gggacgccgt ctcccattcg aggcccttgg ctccccacgg
      781 gttgggttcc gcgatcgggc cgtagcgcat cgaccagatc aggtaaatca gcgggatcaa
      841 atagccgact cccaaaaccg tggctcccgc cgaagacatc acgttcaaca cttgaaattc
      901 cgcgggataa aatgcgtacc ggcgcggcat gcccagataa ccgagaatga actgcggaaa
      961 aaacgtcaga ttaaatccca cgaagaccac cagcgcggca aatttcgccc aaccctccgg
     1021 gtacaggcgg cccgtaatct tagggaacca gaagtgaacg ccgcccaggt aagccattac
     1081 catcccgcct accatcacat agtgaaagtg
//
LOCUS       DATCBB010000742         1454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_24051, whole genome shotgun sequence.
ACCESSION   DATCBB010000742 DATCBB010000000
VERSION     DATCBB010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24051"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VII37_08250"
     CDS             complement(<1..856)
                     /locus_tag="VII37_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5069766.1"
                     /translation="MSQNIRNIAIIAHVDHGKTTLVDAMLRQSGIFRANEALVERVMD
                     SNDLERERGITILAKNTALFFHDTKINIVDTPGHSDFGGEVERALRMVDGVLLLVDAS
                     EGPLPQTRYVLQKALAAKLPPIIVLNKIDRVDARAKEVLDEIYDLFIDLDATEDQLDF
                     PVVYTNARDGLAHRKIGDDSKDLLPLFETIVKSIPSPPGDPNGILQIQVMNLDYSEFL
                     GRIAIGRVFNGTLKRGEDVGIFRLDGKLTSTRITKLYTFRGLERDEADAVAAGDLVAI
                     AGVEGIQIG"
BASE COUNT          335 a          402 c          366 g          351 t
ORIGIN      
        1 cgccgatctg aatcccttcc acgcctgcaa tggccactag atcgccggcg gcgacagcgt
       61 cggcttcgtc gcgctccagc ccacggaagg tataaagctt ggtgatgcgc gtcgaggtca
      121 gttttccgtc gagtctaaaa attccgacat cttctccgcg cttcaaggtg ccattaaaaa
      181 cgcggccgat ggcaatccgt cccagaaatt cgctgtaatc caggttcatt acctgaattt
      241 gtaaaatgcc attgggatcg cccggtggcg aaggaatgga tttgacgata gtttcaaaca
      301 gcggaagcaa atccttggag tcatcgccga ttttgcgatg ggcaaggcca tcgcgcgcgt
      361 tggtgtagac cacaggaaaa tccagctgat cttccgtggc gtccagatcg ataaacaaat
      421 cgtaaatttc gtccagaact tccttcgcgc gcgcatccac gcgatcgatt ttgttcagca
      481 caataatcgg aggcagcttt gcagccagag ctttttgcag tacatagcgc gtttgcggca
      541 acggaccttc gctggcatcc acaagcagga gcacgccatc gaccatgcgc agcgcgcgtt
      601 ccacttcgcc gccaaagtca ctgtgacccg gggtgtccac aatgttgatt ttggtgtcat
      661 gaaaaaacag cgcggtgttt ttggcgagaa tggtgatacc gcgttcgcgt tccaaatcgt
      721 tggaatccat cacgcgttcc actagcgcct cattggcgcg aaaaatcccg ctttgccgca
      781 gcatggcatc cacgagggtg gtcttcccgt ggtctacgtg cgcgatgata gcgatgttgc
      841 gaatattttg actcataaaa ttttagcgtc gagtggccgc gtgacggcca gccattaaag
      901 acaaaaatag ctgctgaaac aaccagcagc ccggacggtt ctcctcatat tctcaggtgt
      961 ggtcgagctc tgtcagttta tcagagggaa ccgtgtgtaa ccagcattct gacacttgtg
     1021 atgccaggaa ccggtaattc ccgagttatt cgtcaacttg acggccccac agcccgatgc
     1081 tagattgcgc tcctgctcga taaacacggg ggctttcact gatccggcgt tccaagaaac
     1141 gcggttctat cggtatcagc cgccgcgaat tcctgcaata tttccaagcc gcacccctcg
     1201 catttctgcc cgctgcgatc tcctcgtttc cattaatgcc gttgtattca ttgaaagaat
     1261 cccgctccgc tgatgatttt catgttcatc ctcgctatcg catgccacga ggattcgaag
     1321 caatgttgcg aaaagtgccg gccggttttg acgaatttgt caccgaaaaa tatcaccatc
     1381 gcgtcgaggc agttttcact gagtggagtg cgcaacttct tcagtcgcca caatcgaccg
     1441 cggcattaga aaaa
//
LOCUS       DATCBB010000743         1151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_444879, whole genome shotgun sequence.
ACCESSION   DATCBB010000743 DATCBB010000000
VERSION     DATCBB010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444879"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..>1151
                     /locus_tag="VII37_08255"
     CDS             535..>1151
                     /locus_tag="VII37_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069767.1"
                     /translation="MKNSLPVFLLLAGLWTSGTALAAQMPAGPAQGPLEQTPPHQPPA
                     AQAAPASTVSDSAPKAAPAMQERYVVPSGTRLPLILHNAVTTRNAKPGDPIYLETLFP
                     IVIDNRILIPAGSYVQGEIQEARRPGRVKGTGEIRLRLNSMILPNGYAVDFNAIPTNA
                     GTGGNEATDSEGKIHGDTDKADDVGTILKSTGVGAGVGGIATQSA"
BASE COUNT          264 a          326 c          324 g          237 t
ORIGIN      
        1 atggaaacgc cggtgtcgtt cggcgcggcg acttgcgccc acgaagcgcg ggcgggaatt
       61 agcatcgcga gaccgagaat cagtatcaga tttttcactt gaatgctcct tgtgtcggcg
      121 aaattggtgg aatctcagcc acccattccg cgcggaatca gctttctgct ttttaattca
      181 ttctttgatt cgttgcgcaa aacagtaggt cacctgtccg tatgtgtcaa ggaaaggctt
      241 gccccacaaa ggtagtgact ccgcttgacc gtgaatatgc cctttccgtc cccgaaaacg
      301 cgggtcggaa cgcctgttac aagaccgttc tgccttgctg gtaaggggtt ttgtgaaggc
      361 cgccccattc ttagggcgga catccgtttg cctccttcag aaccaaacgg agtcactacc
      421 caaatggaag ggaatcgcaa cgtttatcag agtttcaagc ggggcggcag ggcgtataat
      481 cgcgacatcc ccgggttgtg aattcgcatc gaagctgcca tcggagaaaa ccgcatgaaa
      541 aacagcttgc cggtatttct attgcttgcg gggttatgga ccagcggaac tgcactcgcc
      601 gcgcagatgc cggcaggtcc tgcgcagggc cccttggagc aaactccgcc gcatcagcct
      661 ccggcagcgc aagcggcgcc ggcttccacg gtttcagatt ccgctccgaa ggctgcgcca
      721 gccatgcagg aaagatacgt ggttccatcg ggcacgcgcc tgcccttgat cctgcacaat
      781 gcggtgacca cgcgaaatgc caagcccggt gacccgatct atctcgaaac ccttttccct
      841 atcgtaatcg acaaccgaat tctcatccct gccggatcct acgtccaagg cgagattcag
      901 gaggccaggc gccccggcag agtaaaaggc acgggcgaaa ttcgcctgcg attgaattcg
      961 atgattctcc cgaacggtta cgcggttgat ttcaacgcca ttcctacaaa tgccggaacg
     1021 ggcggcaatg aagcgaccga cagcgaaggg aaaattcacg gcgatacgga caaggcggat
     1081 gacgtcggaa ctattttgaa atcgacgggc gttggcgcag gcgttggagg aatcgcgacg
     1141 caaagcgcca a
//
LOCUS       DATCBB010000744         1095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_315605, whole genome shotgun sequence.
ACCESSION   DATCBB010000744 DATCBB010000000
VERSION     DATCBB010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315605"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VII37_08260"
     CDS             <1..446
                     /locus_tag="VII37_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5069768.1"
                     /translation="LETQLAELQTELLARVKDQVTPKGLGGMTLVTLDGEVCDWRRFS
                     NRKQIGSYTGCCPGEHSSGNKRRVGGIDRLGNGRVRALLVEAVWRFLHWQPNWHAALK
                     MKTKLAAGTAMKKKTVIALARQLAIDLWRWRTGRCTMEELGWVPA"
BASE COUNT          258 a          324 c          315 g          198 t
ORIGIN      
        1 cgttggagac ccaactggcg gagctacaga ccgaactgct cgcgcgcgta aaagatcagg
       61 tcacgcccaa gggcctgggc ggcatgaccc tcgtgacctt ggacggcgaa gtgtgcgact
      121 ggcggcgctt cagcaatcgc aaacagattg gcagttacac cgggtgttgt ccgggcgaac
      181 acagttccgg caacaagcgg cgcgtgggcg gcattgatcg gctgggtaac gggcgcgtcc
      241 gcgccctgct ggtcgaggca gtgtggcgct tcctgcattg gcagccgaac tggcacgcgg
      301 ccttgaaaat gaaaaccaag ctggccgcgg gcaccgccat gaagaaaaaa accgtcatcg
      361 ccctcgcccg gcaactggcc attgacctct ggcgctggcg caccggccgt tgcaccatgg
      421 aagaactcgg ctgggtgccg gcataacctt caacccctga tttgattttg tctgattccc
      481 gagaacagag cgtgagcgaa tttgcgaccg ggcggttcct gcccgcagcc ggagctggta
      541 ggcgcaagcc ttgcccgtcg gttagaaggg gcggaacttc cggtcgccgc cgtcacacaa
      601 gtatgacacc gtgggcgttg ggcctcgtcc caactgtccg gataggagct ggtgcgtggc
      661 acctttgcca ggcatgcgtt caagcggcga caccggtcgt tcatactcac gcaacgaact
      721 cagggaaaaa caccgccacc acggcgggga aaggaattac ttggaagagg aataagtgac
      781 ccgagcctgg aaccagcccg acgcgattac tggttagttg gacccatcgc tgcgcgcacg
      841 tcttcgctcc actccgttgc gctccgcgtg acgattacct cactcaaaaa atcttactcg
      901 aacacctcac ttccagttga caaccctcat aggggctccg gcgaattgtt aggcggcgtc
      961 ataacaaata aaatgaaagc tctttgagtg ctgaaaataa agcccatgct ggaaatataa
     1021 ctaaaaaaat cactccgccg cgataactct gcgataatat ccaaaataac ggcaccacaa
     1081 gccacaagct gacgg
//
LOCUS       DATCBB010000745         1049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_218956, whole genome shotgun sequence.
ACCESSION   DATCBB010000745 DATCBB010000000
VERSION     DATCBB010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218956"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VII37_08265"
     CDS             <1..212
                     /locus_tag="VII37_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY5069769.1"
                     /translation="VLNLPAPLESVVGGHAVLAVGYDDAAQRFIARNSWGAKWGQQGY
                     FTIPYTYLMTGSLARDFWTIRMVEG"
     gene            351..>1049
                     /locus_tag="VII37_08270"
     CDS             351..>1049
                     /locus_tag="VII37_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5069770.1"
                     /translation="MNKPIFALTIAAVVISLLTGCNRKPAENSPGPENRHTIAVIPKG
                     TTHIYWQSVKAGAGAAGKEFGYEIQWNGPERETDRERQIQIVEDFIVQKVDGVVLAPL
                     DRDALVPSVEKLASLKIPCAIVDSGIATDKYVTYAATDNYQGGVLAARRLGEVLGGKG
                     NVIVLKYVPGSASTTDRENGFMETIQKEFPGMKVVDSKYGQDTVETALQAAEDMLTKN
                     QDVQGFYACNASTAV"
BASE COUNT          250 a          279 c          319 g          201 t
ORIGIN      
        1 gcgtgctgaa cctgcccgcc ccgttggaaa gcgtcgtggg tggccacgcg gtgctggccg
       61 tgggctacga cgatgcggcg cagcggttca tcgcgcgcaa ctcgtggggc gcgaagtggg
      121 gacagcaggg ttatttcaca attccgtaca cctatttgat gaccggcagc ctcgcgcggg
      181 atttctggac catccgcatg gtggaaggat gagtttaaaa aacatttggg tccgcagtag
      241 ttgtcttccg gcggctgaaa aatctgccgt cagcatcagc ccacctttta gggacaagac
      301 caaccattaa acaattgcca accgtcgttc agataattaa acatgggcgc atgaacaagc
      361 ccatctttgc tctaacgatc gcggccgtcg ttatttctct tttaaccggt tgcaatcgga
      421 agccggcgga aaattcaccc ggcccggaaa acagacacac cattgctgtg attccaaaag
      481 ggacgacgca catttactgg cagtcagtga aggcgggtgc cggggcggcg ggcaaggagt
      541 tcggttatga aattcaatgg aacggaccgg agcgcgagac cgaccgcgaa cgccagatcc
      601 aaatcgtcga ggacttcatc gtgcaaaaag tggacggtgt ggtgctggcg ccactcgacc
      661 gggatgcgct ggtgccgtcg gtggaaaaac tggcgagcct gaagattccc tgcgccattg
      721 ttgattcagg cattgcgacg gacaaatacg tcacctatgc ggccacggat aattatcagg
      781 gcggcgtgct ggcggcgcgg cgtctgggcg aagttctggg cggcaagggc aacgtcatcg
      841 tcctcaaata tgttcccggc tccgcctcaa ccacggatcg cgaaaacggc tttatggaaa
      901 cgatccaaaa agaatttccg ggaatgaagg tcgtggacag caaatacggc caggacacgg
      961 tcgagaccgc gttgcaggcc gcggaagaca tgctgacaaa aaaccaggac gtgcagggat
     1021 tctacgcgtg caatgcgtca accgccgtc
//
LOCUS       DATCBB010000746         1353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_218961, whole genome shotgun sequence.
ACCESSION   DATCBB010000746 DATCBB010000000
VERSION     DATCBB010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218961"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VII37_08275"
     CDS             <1..1185
                     /locus_tag="VII37_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5069771.1"
                     /translation="SQIALVTGDQIIATTLSPSEEKALGQMHADWPGSSKSHELTLGT
                     SSYQAANVLLHAGAPTDVRCYVLMSLEPANAFLRRLNRTIILLGVSAIMLAGLLLSFV
                     SRTITHPLDNLVAGVRALAGGDYGYSINPRGSSEVAELGEAFSKMRGELLASEQRRFA
                     NERIAALGRAASSISHDLRHYLAAVVANAEFLYEAERLKLNRDEIYCEIKTASEQMTD
                     LLDSLRELSRGEGSISPRPASIDHTIRHAVDAVRARPEMRNREISIRSSGDMRGTFDP
                     QKIERAFFNLVLNACEASVSTQGEIEIELHSSAAIFEIRVIDQGSGVPPAIGQTLFDP
                     FVSSGKSNGTGLGLAIVSKVIHDHGGSVAVEATSISGTTFLVRLPRAPQALLETAHAV
                     SL"
BASE COUNT          290 a          367 c          376 g          320 t
ORIGIN      
        1 agtcagatcg cgctggtcac cggggatcaa atcattgcta cgacactgtc gccttccgaa
       61 gagaaggcgc tcggccagat gcatgcggac tggcccgggt ccagcaagtc tcatgagctg
      121 acgcttggca caagttccta ccaggctgct aatgttttgt tacacgcggg ggcgccaacc
      181 gacgtgcgat gttatgtgct tatgtcgctt gagcccgcca acgcgtttct gcggcggttg
      241 aatcgcacca ttattcttct cggcgtgtct gccattatgt tggctggact gttgctgagt
      301 ttcgtttcgc gcaccatcac tcatcctctc gataatctcg tggcgggcgt tcgagcgttg
      361 gccgggggcg attacgggta ctccattaat ccccgaggca gcagcgaagt ggcagagctt
      421 ggcgaagctt tttccaaaat gcgcggcgaa ctgcttgctt cggagcagcg gcgtttcgct
      481 aatgaaagaa tcgctgcgct tggtagggct gccagttcaa tatctcacga cttgcgtcac
      541 tatctagccg cggtggtggc caacgcggag tttctatatg aagcggaaag attaaagctc
      601 aatcgcgacg aaatttattg cgaaatcaaa acggcttccg agcagatgac cgatttgttg
      661 gattcgctgc gcgaactgtc ccgcggggaa ggctccattt caccgcgtcc cgcgtctatc
      721 gatcacacca ttcgtcacgc ggtggacgct gtgcgcgcgc ggccggaaat gcgcaatcgg
      781 gaaatctcca ttcgaagctc cggagacatg cggggaacct tcgacccgca aaaaattgag
      841 cgcgcctttt ttaacctggt gctgaatgcc tgcgaggctt cggtcagtac gcaaggtgaa
      901 atcgagattg aattgcattc ctctgccgca atctttgaga tacgcgtaat cgatcaggga
      961 tccggtgtac cgccggcaat aggccagaca ttatttgatc cctttgtgag ctccggaaaa
     1021 tcaaatggaa cgggcttagg attggccatc gtgagcaaag taattcatga tcacggcggc
     1081 tcagtggcgg tcgaagcgac gtccatatca ggaaccacgt tcttagttag attgccgcgt
     1141 gcgccacaag cgctgctgga aaccgcccac gcagtcagcc tttgactcat ttctttgcag
     1201 cgtaatcact cggctttctt gcccccgcgc cggcacatcg ggacaatttt gtggccgcga
     1261 attgacaagt cctttgctta ttggcgacaa cctgcgactt cccgctaagc acaattgcaa
     1321 ttgaacgaga gcccagcagg agaattctct ggc
//
LOCUS       DATCBB010000747         1121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_154653, whole genome shotgun sequence.
ACCESSION   DATCBB010000747 DATCBB010000000
VERSION     DATCBB010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154653"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VII37_08280"
     CDS             complement(<1..445)
                     /locus_tag="VII37_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5069772.1"
                     /translation="MKKIFTGRRDFLKQAGVMGSMAMMAGMPEGAMALGGAHGRVVEE
                     VALPAQAEEAPKNHIKFAVCGMSHDHVYGMIGAIQRGGGEMVAAWAGEPDKIATFKKR
                     FPNVKMVATQDEILNDASVQLVLSSQIASERAPLAVRAMKHGKDFL"
     gene            755..1078
                     /locus_tag="VII37_08285"
     CDS             755..1078
                     /locus_tag="VII37_08285"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY5069773.1"
                     /translation="MTRYGMVIGLKPEAEEKYKQYHAAVWPGVLEMIHKCNIRNYSIY
                     LKDHTLYAYFEYHGTDYAADMAKMAADKTTQEWWAIMEPMQSPVPTRKEGEWWACMEE
                     VFHLD"
BASE COUNT          233 a          310 c          336 g          242 t
ORIGIN      
        1 cgagaaagtc cttgccgtgc ttcatagcgc ggacggccag aggcgcgcgt tcgctggcga
       61 tctgcgaact caggaccagt tgcacggatg cgtcattcag aatttcgtcc tgggtcgcga
      121 ccattttcac gttcggaaag cgctttttga aggtggcaat cttgtccggc tcgccggccc
      181 atgctgcaac catctcgccg ccgccgcgct gaattgcgcc gatcatgcca tagacgtggt
      241 cgtggctcat gccgcacacg gcaaacttga tgtgattttt gggggcctct tcggcctggg
      301 cgggcagcgc cacttcttcg actacgcggc catgggcacc gccaagcgcc atcgcacctt
      361 ccggcatgcc ggccatcatg gccatcgatc ccattacccc ggcttgcttc aggaagtcac
      421 gccggccagt aaaaatcttc ttcatttctc ctccgcatcc gcaacagttt tgttaaacca
      481 ggtaacgaat tcgactttcc tgaaatgact catccggtcg cgccgaattt ccgaacagag
      541 cgttttccaa ttttcgtccg catcgatgat aaccgggctt gccgggcgat gagaattgcc
      601 aggggtaatt gcggctgcct tggtggggtg aggaggcggg aagaggaggc cggcgggacc
      661 gaggcccgat gatggttaaa tggatggaaa cagcgtgtag gcattatgtg cgcgcgtggc
      721 aattgcggtg ccagaatttt tgcaaggaga tacgatgact cggtacggaa tggtgatcgg
      781 cctcaagccg gaggccgaag agaagtacaa gcaataccac gccgccgttt ggccaggcgt
      841 gctggagatg attcacaagt gcaacattcg caactactcg atctacctga aggaccacac
      901 tctttacgcc tattttgaat accacgggac cgactatgcc gccgatatgg caaagatggc
      961 cgctgataag acgacgcagg agtggtgggc gattatggag cccatgcaga gccctgtgcc
     1021 gacgcgcaaa gagggcgagt ggtgggcctg catggaagag gtttttcatt tggactgagg
     1081 tggatgcggt cctatccttg ccgcattccc agcgcccgcc g
//
LOCUS       DATCBB010000748         1347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_380364, whole genome shotgun sequence.
ACCESSION   DATCBB010000748 DATCBB010000000
VERSION     DATCBB010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380364"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /gene="nadD"
                     /locus_tag="VII37_08290"
     CDS             complement(<1..702)
                     /gene="nadD"
                     /locus_tag="VII37_08290"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY5069774.1"
                     /translation="MTTAQAHRTAAKHHPRRIAVFGGSFDPIHNGHLAIARAADRRFN
                     FDEIHFIAASRPPHKLKQHLAPFPHRFAMVSLACTEHPHFVPSLAEAGEDFSGSQLHY
                     SVDTVRYFRHTYNGAGDRVFFIIGADAFLDIPMWKEYETLLGLCDFIVANRPGIRPEA
                     LRLVIPPDAIARPDLKKETEAAHPSQVVAHLNRSTVYLLENVNSDISATDIRRRANRG
                     QSIHGLVAARVEEYIL"
     gene            complement(764..>1347)
                     /locus_tag="VII37_08295"
     CDS             complement(764..>1347)
                     /locus_tag="VII37_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY5069775.1"
                     /translation="YPFTTLEPNLGVVNADGGTGAHGTELGRTFVVADLPGLIEGAHE
                     GAGLGMRFLRHVERTRLLVHLIDTSDSNDDDPIHSYEVISGELAAFSKTLTEKPLIVV
                     ATKLDATTDRTRLEALRDFCRKEHLEFHSISAATGEGVKELVRSIADALDKIPRHAPD
                     TADDQVLDSPQVIPESAGERDVHAPLVSRDEKS"
BASE COUNT          289 a          358 c          404 g          296 t
ORIGIN      
        1 cagaatgtat tcctccacac gagcggccac aagaccgtga atggattgac cgcgattcgc
       61 gcgacggcga atatctgttg cggaaatatc gctgtttacg ttttccagaa gatagaccgt
      121 gctgcgattc aagtgcgcta ccacctgtga aggatgcgca gcttcggttt ctttttttaa
      181 atccgggcgc gcaatcgcgt cggggggaat caccaggcgc agcgcctccg gccgaatgcc
      241 cgggcgattg gccacgatga aatcgcacaa ccctagaagc gtttcgtatt ccttccacat
      301 cggaatgtcc agaaacgcgt ccgctccaat gatgaaaaaa actctgtcac cggcgccgtt
      361 gtaggtgtgc cgaaaataac gcaccgtatc gaccgaatag tgcagctggc tgccgctgaa
      421 atcttcccca gcttcagcca gcgacggcac gaagtgagga tgttccgtac aagcgagaga
      481 gaccattgca aagcggtgcg ggaaaggcgc gagatgctgt ttcagtttgt ggggcggccg
      541 gctggccgcg atgaaatgaa tttcgtcaaa attgaagcgc ctgtctgccg cgcgagcaat
      601 ggccaggtgg ccgttgtgaa tgggatcgaa agagccgccg aaaacagcga tgcgacgcgg
      661 atgatgtttt gcagcggtgc ggtgggcttg cgcggtggtc aatgcttctc cgggattaaa
      721 gtcgctgtcc agactgcggt ccgggcggca tatggtaatt ctctcacgat ttttcgtcgc
      781 ggcttacaag tggcgcatga acgtcacgtt cgccggcgct ctcaggaata acttgcggtg
      841 agtccaacac ttgatcgtcg gcagtatcgg gcgcatgtct gggaattttg tccagtgcgt
      901 cggcaataga acgcaccagt tctttcacgc cttcgccggt ggcggcggaa atggagtgga
      961 attccagatg ttcttttcgg caaaaatcgc gcagtgcttc aagtcgcgtg cggtcggtcg
     1021 tagcgtcaag cttggtggcc accacgatga gaggcttttc tgtcagagtt ttgctgaaag
     1081 ctgccagctc tcctgaaata acttcataag agtggatggg atcgtcatcg ttcgaatcgc
     1141 tggtgtcgat cagatgaacg agcaagcgcg tacgctccac gtgccgaaga aaacgcatgc
     1201 ccagccctgc gccttcgtgc gcgccctcga tcagtcccgg aagatccgcg acgacgaatg
     1261 tgcgccctaa ttccgttccg tgcgcgccgg tgcccccgtc ggcgttcact acaccgaggt
     1321 tgggctccag agtggtgaag ggataat
//
LOCUS       DATCBB010000749         1958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_88578, whole genome shotgun sequence.
ACCESSION   DATCBB010000749 DATCBB010000000
VERSION     DATCBB010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88578"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="ftsW"
                     /locus_tag="VII37_08300"
     CDS             <1..1071
                     /gene="ftsW"
                     /locus_tag="VII37_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY5069776.1"
                     /translation="ALCLLGAVMVFSASAVTAEQHYGRSYIFLLRQAAWLVIGLFGMF
                     ALMRLDYRRLREPKVVYTALVTVFLMLVGAFFLDKSHATHRWIKFGPVGIQPSELAKL
                     AVILYLAWFLDRKRRSAAKMEFCKHDLVQTILPAVGPILLCVGVILLQPDLGTSVDIV
                     VIATAILFVAGLSWKWIACGLAGALPILYLLITHVSYRQARLMAFLNPASDPQGSGFQ
                     LLQSLIAVGSGGFSGVGLMESKQKLFYLPEAHTDFIYAVICEELGFIGAMLVIGLFVV
                     YGWRGLRASFTAPDGFGRLLALGITAMVISQALINFAVVLGMVPTKGIPLPFVSYGGS
                     SLLVMLLATGVLLNISQQATEQ"
     gene            1148..>1958
                     /locus_tag="VII37_08305"
     CDS             1148..>1958
                     /locus_tag="VII37_08305"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEY5069777.1"
                     /translation="MGLKLLIAGGGTGGHVFPAMAIAREWLSRGSDREVVLVGTARGI
                     EMKLVPQAGLPLETLRVAGLKGKGGSTLLKNLFKLAPAMLDASRVFKKHKPIAAFGVG
                     GYAAGPMLLTTWLRGIPNVIFEPNAEPGFTNKSLARISNRIATGYEISARTWGAKAVV
                     TGCPVRREFLSIAQRTPQKPLRVLITGGSQGALPVNRAFVDAMNFLATRKNEMSIVHQ
                     TGERDYDAVRTAYARHEILAEVVPFLGNMPERFAWADIIVCRAGAITAAEVA"
BASE COUNT          369 a          483 c          633 g          473 t
ORIGIN      
        1 gcgctgtgcc tgcttggcgc ggtgatggtg ttttcggctt cggcggtcac agccgaacag
       61 cattacggac gctcttatat ttttctgctg cggcaagcgg cttggttggt tatcggcctc
      121 tttggaatgt ttgcgctgat gcgcttggat tatcgccgct tgcgcgagcc caaagttgtt
      181 tatacggctc tggtcacggt gtttttgatg ctggtcgggg cattctttct ggataaatcc
      241 catgctacac atcggtggat caagttcggt ccagtgggga ttcagccgtc ggagttagcg
      301 aaactcgcgg tgattttata cctggcatgg tttctggacc gcaagcggcg cagcgccgcg
      361 aagatggaat tctgcaaaca cgatttggtg cagaccattt tgccagcggt cgggccgatt
      421 cttttgtgtg tcggggtgat attgctacag cccgacttgg gtacttccgt ggacatcgtg
      481 gtgatcgcca cggcgattct tttcgtagcg ggactttcct ggaagtggat tgcctgcgga
      541 ctagcgggcg cgcttccgat tctttatctg ctgatcacgc acgtttcgta tcgccaggcg
      601 cggctgatgg cgtttcttaa tccggcatcc gatccgcaag gctcaggatt tcaattgtta
      661 caatccttga tagccgtggg ttcgggcgga ttcagcggcg tgggcctgat ggagagcaag
      721 cagaaattat tttatttgcc ggaagcgcac accgatttta tttacgcggt gatttgcgaa
      781 gagctcggtt ttatcggggc catgctggtg atcggtttgt ttgtggttta cggctggcga
      841 gggttgcgcg cctcgttcac tgctccggat ggatttgggc ggctgttggc gctgggtatc
      901 accgccatgg ttatcagcca ggcgctgatt aattttgcgg tggtgttggg gatggtgccc
      961 accaagggta ttccgctgcc gttcgtgagt tatgggggat cgagtttgct ggtgatgctg
     1021 ctggcaaccg gcgtgctcct gaatatcagt cagcaggcga ctgagcagtg agccacaaaa
     1081 cgataagcca tcgagccgtg aggatggcgc gcgcagagaa gatttgtgtg gcgtgggctt
     1141 ggatgaaatg ggactgaaat tgttaattgc gggcggcggc accggcgggc atgttttccc
     1201 ggcgatggct attgcgcggg agtggttgtc acgcgggagc gaccgggaag tggtgctggt
     1261 gggaacagca cgcggaatcg aaatgaagct ggtgccgcaa gcggggctgc cgctcgaaac
     1321 gttgcgtgtg gcaggactga aaggcaaagg cggaagcacg ctgctgaaga atcttttcaa
     1381 gcttgcgccc gccatgctgg acgcttcgcg agtattcaag aaacataaac ccattgcggc
     1441 ctttggcgtg ggcggatacg ccgcggggcc aatgctactg actacctggc tacgcggaat
     1501 tcccaacgtg atcttcgagc ctaacgccga accaggattc accaacaaat cgttggcgcg
     1561 catttccaac cgaattgcca ccgggtatga aatttctgca cggacttggg gagcgaaagc
     1621 ggtagtcacc ggatgcccgg tgcgccggga atttctgagc atcgctcaac gtactccaca
     1681 aaagccgctg cgagtactga ttaccggagg cagccagggc gcattgccgg tcaatcgcgc
     1741 gtttgtcgac gccatgaatt ttctggccac caggaaaaat gaaatgagca tcgtgcacca
     1801 gaccggcgag cgcgactacg atgcggtgcg caccgcttac gcgcggcacg aaattctcgc
     1861 ggaggtggtg cctttcctgg gcaacatgcc ggaacgcttt gcatgggcag atattatcgt
     1921 gtgccgcgcg ggcgcgatca ccgccgcgga agttgccg
//
LOCUS       DATCBB010000750         1053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_380373, whole genome shotgun sequence.
ACCESSION   DATCBB010000750 DATCBB010000000
VERSION     DATCBB010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380373"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..>1053)
                     /locus_tag="VII37_08310"
     CDS             complement(180..>1053)
                     /locus_tag="VII37_08310"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY5069778.1"
                     /translation="MELHTLGVEGGYTQKDVTELARCFTGWTIDKPRLNPVFKFDDKL
                     HDPTQKIVLNKKIHAGGMKDGEQAIELLSKHPSTAKFISTKLARRFVSDTPPAPLVAR
                     MAKTFESSDGDIHAVMHTMIYSPEFWSREEYRAKIKTPFELVVSSVRALGTDVDTAMP
                     LVQWVGRIGEPLYQCQPPTGYADNAEAWVNTGALLNRLNFSLTLAGNKVRGSRADVSS
                     LLGADAPEPKVALERALQVFLGGQAGPTTLETLQKQLDSPQVLQAKLDDPVKKVDLGV
                     VTGLVLGAPEFQRR"
BASE COUNT          223 a          289 c          299 g          242 t
ORIGIN      
        1 tggcagcgac ggcgcgttgc aagaacgcgg gcatggcgct caggcctacc atggccacgc
       61 caccttgttt caaaaaatat cgccgggaaa atctcatgtg cgactccttc tgctaacaac
      121 accaactcaa cggcacctca atatcaactc gacatcaaac cggtgccgaa aagccacggt
      181 tatcgtctct gaaattctgg agcgccgaga acaagcccgg tcactactcc gaggtcaact
      241 ttttttactg gatcatcgag cttggcttgc aggacctgcg gactgtctag ttgcttttgc
      301 agcgtctcca gcgtcgtcgg tccggcctgg ccgcccagaa aaacctgcag cgcacgttcc
      361 agcgcaactt ttggctcggg ggcatccgcg cccagcaatg aactgacatc ggcgcgcgag
      421 ccacgaactt tgtttcccgc cagcgtcaaa gaaaaattca gacgattcag gagtgcgccg
      481 gtattgaccc acgcttccgc gttatccgcg tagcccgtgg gcggttggca ttgatacagc
      541 ggctcaccga tgcgtccgac ccattggacc aggggcatgg cggtgtctac gtcggtgccg
      601 agcgcgcgaa cggaagacac tacgagttcg aacggcgttt taatttttgc gcggtactct
      661 tcacgagacc aaaactccgg cgaataaatc atggtgtgca tgaccgcgtg aatgtcgcca
      721 tcgctagatt caaatgtttt ggccatgcgc gcgactagcg gggcgggagg agtatcggag
      781 acaaagcggc gcgccagttt tgtggagata aatttggcgg tactgggatg cttcgagagg
      841 agttcgatgg cctgttcgcc gtctttcatt ccgccggcgt ggatcttttt gtttaagaca
      901 attttttggg tgggatcgtg caacttgtcg tcaaatttaa atacgggatt cagtcgaggc
      961 ttgtcgatgg tccagccggt gaagcagcgg gccagttcgg ttacgtcttt ctgcgtgtag
     1021 ccgccttcca cgcccaaggt gtgaagttcc atc
//
LOCUS       DATCBB010000751         1124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_315774, whole genome shotgun sequence.
ACCESSION   DATCBB010000751 DATCBB010000000
VERSION     DATCBB010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315774"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VII37_08315"
     CDS             <1..1117
                     /locus_tag="VII37_08315"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY5069779.1"
                     /translation="LLGDSLLPDSKTDGADFCRARLALPLALFRKMLRFLGWEIQRNA
                     GAPWRGLTVYYVDGTTLPLFNSKKNRAAFGGSRNQNGESRMPIARVVLLVSAGAIVDL
                     VCGPYAASELRLFYSLLRQLSPESLVVGDTLYNSFFNMASARARGSHMLCRSRAQRHA
                     NVIGTLGRGDRLHRWTKPQREHTLIASAWESLPSTLTVRVITRTVRRKGYRDYVLTLT
                     TTMLDPVRYPAEELVALYLSRWNIELDLRTLKAVHGWARLKCKTPQSVHREIFSGALA
                     FNAVRLLMSQSGKPIRSLSHSGANQTLIETASRMSAARTESLPAMLNHALANIARQIL
                     DKTARLPQPRLLAYNLRRYGFLKTSRAQWRKENDVAS"
BASE COUNT          228 a          337 c          320 g          239 t
ORIGIN      
        1 ccttttaggc gacagtcttt tgcctgattc taaaaccgat ggcgccgatt tttgccgggc
       61 gcgcctggcc cttccgcttg ccttgttccg taagatgctg cgctttctgg gatgggaaat
      121 tcagcgcaac gcgggagcgc cgtggcgggg actcacggtt tattacgtgg atggaaccac
      181 cctgccgctg ttcaattcga aaaagaaccg cgcggccttt ggcggctccc gcaatcaaaa
      241 cggcgagagc cgcatgccga tcgcgcgcgt ggttttattg gtcagcgcgg gcgcgattgt
      301 cgatctggtc tgcgggccgt acgccgccag cgaattgcgt ttgttttatt cgctgctgcg
      361 gcagctctcg cctgaatcgc tggtcgttgg cgacacgctt tacaatagtt ttttcaacat
      421 ggcttcggcg cgggcgcgcg gttcgcacat gctctgcagg agccgcgccc aacgccatgc
      481 gaatgtgatt ggaactctcg ggcgcggcga tcgcttgcat cgctggacta aaccgcagcg
      541 agaacacacc ctcattgcct cggcatggga gtcgctgccg tccacgctga ctgttcgcgt
      601 tatcacccgc acagttcggc gcaaaggcta ccgcgattat gtgctgacgc tcaccaccac
      661 aatgctggat ccggtccgct accccgcgga agaactcgtg gcgctttacc tttcacgctg
      721 gaatattgaa ctggacttac gaacgctcaa ggcggttcat ggctgggcga ggctcaaatg
      781 taagaccccg cagagcgtcc accgcgaaat cttttctgga gcattggcgt tcaacgcggt
      841 tcggctgctg atgtcgcaaa gtggaaaacc catccgcagc ctcagccact cgggagcgaa
      901 tcaaaccctg atcgaaacag cgtcgcgcat gagcgccgcg agaacggagt cgctgccggc
      961 aatgctcaat cacgcgctcg ccaacatcgc acgacagatt ttggataaaa cagcgcgcct
     1021 gcctcaaccg cgcctgctgg cctacaactt aagacgctat ggatttttga aaacctcccg
     1081 cgcgcaatgg agaaaagaaa atgatgtcgc ctcgtgaagc agca
//
LOCUS       DATCBB010000752         1482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_154794, whole genome shotgun sequence.
ACCESSION   DATCBB010000752 DATCBB010000000
VERSION     DATCBB010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154794"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VII37_08320"
     CDS             complement(<1..829)
                     /locus_tag="VII37_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY5069780.1"
                     /translation="MAVKANNSSTMKNSSTQKWGPQTRSVHAGELNKHGVGAPVGTEI
                     CRSSTFTFSSTEEMKLWAEGKSKAYIYTRYGNPTLTVAQEKIAALEGAEAAVVTASGM
                     AAISCALLGTLKAGDELISTAQLYGGSYRLMRDIFPDLGIKVHHVETDLAGMEEKLSS
                     KTKVLYVETPTNPMLRLVDLHKAVAFAKKHELVSIIDNTFATPVLQNPIAMGYDMVVH
                     SATKALAGHSDIIAGAVTGSATWMKRIQHMVISLGGSMDPEAAFLLIRGMKTLGLRVA
                     "
     gene            complement(870..>1482)
                     /gene="fusA"
                     /locus_tag="VII37_08325"
     CDS             complement(870..>1482)
                     /gene="fusA"
                     /locus_tag="VII37_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5069781.1"
                     /translation="SGGHGQFGVCRIKMEPTERGKGIEFVDDIFGGSIPKNWIPSVEK
                     GIRDAAARGYLAGFPVVDFRVSLYDGKYHDVDSSDMAFKIAGSLAFKEAMKAAKPALL
                     EPIMQVEVYAPDQYSGDLMGDLSSRRGRIAGSDLRGHNVVIKAQVPFAEMLTYANDLI
                     SKTQGRATYTMEFSHYDYVPNELAEKVIATHKPHADQHADDEA"
BASE COUNT          264 a          407 c          460 g          351 t
ORIGIN      
        1 gcgccacgcg caggcccagc gtcttcattc cgcgaatcag caggaaggcg gcttccgggt
       61 ccatcgaacc gcccagggaa atcaccatgt gctggatgcg cttcatccag gtggcgctgc
      121 cggttacggc tcccgcgatg atgtcggagt ggccggcgag cgctttggtg gcgctgtgca
      181 ccaccatgtc gtagcccatg gcgatgggat tctgcagcac cggcgtggcg aaggtgttgt
      241 cgatgatgga aacgagttca tgtttttttg cgaacgccac cgctttgtgc agatccacga
      301 gacgcagcat gggattcgtg ggcgtctcga catacaggac tttggtcttg gaagataatt
      361 tttcttccat gccggccaga tcggtttcca cgtggtggac tttgatgccc aggtcgggaa
      421 agatgtcgcg catcagccga tagcttccgc cgtatagctg cgcggtggag atcagttcgt
      481 ctccggcttt cagcgtgccg agcaacgcgc acgaaatggc ggccatgcct gacgcggtaa
      541 ccaccgcagc ctccgcgcct tcgagcgcag cgattttttc ctgcgccacg gtaagcgtgg
      601 gattgccata tctcgtatag atgtacgctt tgcttttgcc ttcggcccag agcttcattt
      661 cttccgtgga agaaaatgta aaagtggagc tgcggcatat ctccgttccg accggagcgc
      721 cgacgccgtg cttgttcagt tcgccggcgt ggactgagcg tgtctgcggg ccccacttct
      781 gcgtgcttga atttttcatg gtgctggaat tgttggcctt cacggccatt cgcggcaagc
      841 gccattttgg cgtcccgcgg acgccggtgc taggcttcgt cgtctgcgtg ttgatcggcg
      901 tgcggtttgt gagtggcgat tactttttcc gccagctcat tgggaacgta gtcgtagtgg
      961 gaaaattcca tggtgtaggt ggcgcgcccc tgtgttttgg aaatgaggtc attcgcgtag
     1021 gtcagcattt cggcgaaagg aacctgggcc ttgatcacta cgttgtggcc acgaagatcg
     1081 ctgccggcga tgcgcccgcg acgcgagctc aggtcgccca ttaagtcgcc ggaatactgg
     1141 tcgggagcat aaacctcaac ttgcatgatg ggctcaagca gcgcgggctt cgcggctttc
     1201 atggcttctt tgaaggccag cgagccggcg attttgaaag ccatgtcgct tgaatccacg
     1261 tcgtgatatt tgccgtcgta gaggcttacg cgaaaatcga cgactgggaa tcccgcgagg
     1321 tagccgcgcg cggcggcgtc gcgaatgccc ttttcaacgg agggaatcca gttctttgga
     1381 attgacccgc caaaaatatc atcgacgaat tcaatgcctt tgccgcgttc ggtgggttcc
     1441 atcttgatgc ggcagacgcc gaactggccg tggccgccgg ac
//
LOCUS       DATCBB010000753         1339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_445178, whole genome shotgun sequence.
ACCESSION   DATCBB010000753 DATCBB010000000
VERSION     DATCBB010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445178"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1339)
                     /locus_tag="VII37_08330"
     CDS             complement(<1..>1339)
                     /locus_tag="VII37_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY5069782.1"
                     /translation="ELMAIDIAGALYANASERERLKKLLQEHGAVADFEFEMRRKDGE
                     IRTVLESSIAVRDNSGNVTAYQGFVLDISERKRAEHEIRRRNRELLVLNSIGQTLTES
                     MDLTDSLRRTLSQLTELFSLDTSSLYLFDDGGSTLRRVAAVGQRSEYARHLSPVTLQP
                     ELIDHIKSVHATFLSVHGLSLPPIFRDALTKEGIVSAYIVVLWSKGRPLGGLVVGSRT
                     SREFSSADVNLLIAVGSQISNATERALLYDESRQAYENVRRTQEQLLNSEKMAAVGQL
                     ISGVAHELNNPLTAILGYSQLLSSSGQVGPQGIEYSEKLYKQAQRTHRIVQNLLSFAR
                     QHKPERIPTRINQILEDTLALRDYDLRMGNIRVHLDLAQDLPSMSADPHQLQQVFLNM
                     VNNAVDAILESSEDGDLWVCTRSDSEKVYVEFTDSGPGVQEASRVFDPFYTTKP"
BASE COUNT          285 a          365 c          407 g          282 t
ORIGIN      
        1 ccggcttggt agtgtaaaaa ggatcgaaaa cgcgagacgc ttcctgcaca ccaggaccgc
       61 tgtcggtaaa ttccacgtaa actttttcgc tgtctgagcg ggtgcacacc cacaggtcac
      121 cgtcttccga actttccaga atggcgtcga cggcattgtt caccatgttg agaaacacct
      181 gctgcaactg gtgcgggtcg gcagacatcg acggaaggtc ctgcgccaaa tccaggtgca
      241 cacggatatt ccccatgcgc aagtcgtaat cgcgcagggc cagggtatct tcgagaatct
      301 gattgatgcg cgtgggaatg cgctcgggct tgtgctggcg ggcgaagctc aagagatttt
      361 ggacgatgcg atgggtgcgc tgcgcctgct tgtagagttt ttccgagtat tcgattccct
      421 gcggccctac ctggccactg gagctaagca actggctgta acccaggatg gcggtaagcg
      481 gattgttcaa ttcatgagcg acgccggaaa tcaactgacc tacagccgcc attttttcgc
      541 tattgaggag ctgttcctgg gtgcggcgca cgttctcata ggcctggcgg gattcgtcat
      601 acaaaagcgc gcgttcggtg gcattggaga tctggctgcc gacggcaatg aggagattga
      661 cgtcggcgga tgaaaattca cgcgaggtgc ggctgccgac aaccaacccc ccgagcgggc
      721 ggcctttaga ccacaacacc acgatgtacg cggaaacgat gccctcttta gtcagggcgt
      781 cgcgaaaaat aggaggaagc gaaaggccgt gaacggacaa aaatgtggcg tgcacgctct
      841 tgatgtggtc aatgagctcg ggctgcaggg tcaccggtga aagatggcgc gcgtattctg
      901 aacgctgccc gaccgccgcg acacgccgca gcgtgctgcc accgtcatcg aacaggtaca
      961 gggaagaggt atcgaggctg aacaactccg tcaactggct gagggtgcgg cgcagcgaat
     1021 cggtaaggtc catcgattcg gtaagcgttt gcccgatgga attcaatacc agcagctcgc
     1081 gatttctgcg gcgaatttcg tgctcagccc ttttccgctc gctgatatcc aggacgaagc
     1141 cctgataggc ggtgacgttg ccggaattgt cgcggaccgc gatggaggat tccagcacgg
     1201 ttcttatttc tccatctttg cggcgcatct cgaactcgaa gtccgcgacg gcgccatgct
     1261 cctgcaacag tttcttcagc cgctcgcgtt cagaggcgtt ggcgtagagc gcgccggcga
     1321 tatcgatggc catcagttc
//
LOCUS       DATCBB010000754         1969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_154934, whole genome shotgun sequence.
ACCESSION   DATCBB010000754 DATCBB010000000
VERSION     DATCBB010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154934"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VII37_08335"
     CDS             complement(<1..302)
                     /locus_tag="VII37_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069783.1"
                     /translation="MVAVAAGIVHTVPMNNHKNDQNTEMQTATQRNRITGEANLTNRI
                     VAVNRAPQGFDPKRDLPAGFVGFLRPLHAALTARQQVLAERRAVALAESNAGKLPE"
     gene            475..>1969
                     /locus_tag="VII37_08340"
     CDS             475..>1969
                     /locus_tag="VII37_08340"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069784.1"
                     /translation="MSPSTLKPDITQANEIPHDFLAQIKEHIANRRIDAGLRCLTAHQ
                     QLLESIPLRPNNSVILLGYFAQWVDVGFPGRPLLKKLLSHFASASPESLTLLEYAHLR
                     MADGLVAMSEEEFPKAIKSFNTVLTLENEISDKQMIAIANFWMGRCLRRQGRYEDALS
                     FVAKGRDLAMQLKYPKMAAVMQVLEGWVAFQEGHPEQAAKILGEAEEVLADTDDYVTL
                     GNISSAYGRIARRQGNSEHALSKFEKAIELYNKRDPYNRNLARSFVNIAFVKRLLALQ
                     LGNKIDSEAARLRKKAAKKSKANSKSSPPSKPPSREQRTQLRQEAFEHLTKAQEIYQR
                     YDDHRGNGNVHITLGYLNLDDGELDRAAVQAATAFRLGAEKKDNVLKARARMLQSSVQ
                     GAMFEGQIEESSSRVPSSQLACEFAREALESARHTQNRRLIAKAHISLGLALCLDFPD
                     DLEAIQQCIDGAAALLKPAHQDYVWRELQDLKRKLRGAGNVNATLREW"
BASE COUNT          464 a          654 c          477 g          374 t
ORIGIN      
        1 tactccggca atttccccgc attggattcc gccagcgcca ctgcccgacg ttccgccagc
       61 acctgctggc gcgcagtcaa cgccgcatgc agtggccgga gaaacccaac aaatccggcg
      121 ggcaggtcgc gcttcggatc aaacccttgc ggcgcgcgat tcaccgcaac gatgcgattc
      181 gtgagattcg cctcgcccgt tatgcggttt cgctgtgtgg cagtttgcat ctcggtgttt
      241 tggtcgtttt tgtggttgtt catcggaaca gtatgcacaa tcccggcagc cacagcgacc
      301 atgatggagt agactccaga attgggttcc ggtcaaaggc actagtccga ttcggacgtg
      361 tgcccagttt gaccatttcc ccacccgcgg caaaagccgc gcctgcagag tttcgcgaag
      421 accgctgttt ttgaatgttt tcacgaggcg gaaccttcga gtgcttagaa aaatatgtct
      481 ccctcaacgc tcaaacccga tatcactcaa gcaaacgaaa tcccccacga cttcctcgcc
      541 caaatcaagg aacacatcgc caaccgtcgt atcgacgcgg gcctgcgttg cctaaccgca
      601 caccagcaac tcctggaatc catcccgctg cgcccgaaca attccgtcat cttgctcggc
      661 tatttcgccc aatgggtcga cgtcggattt cccggacgcc cgctcctaaa aaaactgttg
      721 tcgcattttg caagcgcttc acccgaaagc ctcacgcttc tcgagtacgc ccacctgcga
      781 atggccgacg gcctggtcgc catgtccgaa gaagaattcc ccaaggccat caaaagcttc
      841 aacaccgttc tcaccctgga aaacgagatc agcgacaaac aaatgatcgc catcgccaat
      901 ttctggatgg gccgctgcct tcgccgtcag ggccgctacg aagacgccct cagcttcgtt
      961 gctaaaggca gagatttggc catgcagctc aagtacccca aaatggccgc ggtcatgcaa
     1021 gttctcgaag gttgggttgc tttccaggaa gggcacccgg aacaagccgc caagattctc
     1081 ggcgaagccg aggaagtcct agccgacacc gacgactatg tgacgctcgg aaacatcagt
     1141 tccgcgtatg gcagaatcgc ccgccgccag ggcaattccg aacacgcgct ctccaaattc
     1201 gagaaggcca tcgagctcta caacaagcgc gatccctaca atcgcaatct ggcccgctcc
     1261 ttcgtcaata tcgcgttcgt caaacgcctg ctcgcccttc aactgggaaa taaaatagat
     1321 tcggaagccg ccagactcag aaaaaaggcc gccaaaaaaa gtaaagcgaa ttccaaatct
     1381 tcgccaccat ctaagccgcc ctcccgcgag cagcgcaccc agcttcgtca agaggccttc
     1441 gagcacctca ctaaagccca ggagatttat cagcgctacg acgatcaccg cggcaatggc
     1501 aacgtgcaca tcactctcgg ctacctaaac ctcgacgacg gcgaattgga tcgcgccgca
     1561 gtccaggccg ccaccgcctt ccgcctcggc gcagaaaaaa aggacaacgt cctcaaggct
     1621 cgcgcgcgca tgctgcaatc ttccgtgcaa ggcgccatgt ttgaaggcca aatcgaggaa
     1681 agctcaagcc gcgtgccctc ctcccaactc gcctgcgaat tcgcacgcga agctctggaa
     1741 tccgcccgcc acacgcaaaa tcgccgcctc atcgccaaag cccacatctc tctagggttg
     1801 gcgctctgcc tcgattttcc cgacgatctc gaagccatcc agcagtgcat cgacggcgcc
     1861 gccgcattgc tcaaacccgc tcatcaggat tacgtctgga gggaacttca ggatctaaaa
     1921 cgcaaacttc gcggcgccgg caacgtcaat gccacgctcc gcgaatggt
//
LOCUS       DATCBB010000755         1040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_122279, whole genome shotgun sequence.
ACCESSION   DATCBB010000755 DATCBB010000000
VERSION     DATCBB010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122279"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VII37_08345"
     CDS             complement(<1..730)
                     /locus_tag="VII37_08345"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HEY5069785.1"
                     /translation="MKRFRNFPIQQKMLVMTLLICGAVLLVAIASLFVFQVLNFRSNF
                     QRDTSTLAAIIANQSTAVMQFKDDATATEIVDSLQAKPTILSASLVLPDRSILAHYGI
                     TEQAIDLAKFPPPGEPRFIGEYLLLTQPVVLKGEQVGTLYLRSNYQQTFLKLLTFYSL
                     VIAGILIVSISLAVFLSGRLGRTITQPVLELARTAQIVGEQKNYSVRAVVGGRRDELG
                     RLAESFNEMLSRIESQDAALNLSQQ"
     gene            complement(735..878)
                     /locus_tag="VII37_08350"
     CDS             complement(735..878)
                     /locus_tag="VII37_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069786.1"
                     /translation="MNWFWDIFIKRKLLATSAAVSTVASLLVAGGFVGANGWEVITRV
                     NHS"
BASE COUNT          241 a          262 c          303 g          234 t
ORIGIN      
        1 tttgttgcga taaattgagc gctgcatcct ggctttcgat gcggctcaac atctcattga
       61 aggattccgc gagacggccc agttcatcgc gccggccgcc cacgaccgcg cgaaccgagt
      121 aattcttttg ctcgccaaca atttgagcgg tgcgcgccag ttcaaggacg ggttgcgtga
      181 tcgtgcgtcc caaccggcca gaaaggaaca ccgccaggct gatggacact attaaaatgc
      241 cggcgatgac cagactataa aaagtcagca gtttcagaaa tgtttgctgg tagtttgatc
      301 gcaagtaaag tgtgccgact tgctcccctt tcaaaacgac cggttgagtg agcagaagat
      361 attcaccgat gaaccgcggt tctcccggtg gcggaaattt tgccagatca atcgcctgct
      421 ccgtgattcc gtagtgggct aaaatagatc tatcaggcaa aaccagcgag gctgacaaga
      481 tggtaggttt ggcctgaaga gagtccacga tttcggtcgc ggtcgcgtca tctttaaatt
      541 gcatcacggc ggtgctttga ttggcgatga tggctgccag cgtggaggtg tcgcgctgga
      601 agttggaacg gaagttcagg acttggaaaa caaaaagcga tgcgatggcg accaaaagga
      661 ccgcgccgca gatgagcagg gtcatcacca gcattttctg ttgaatgggg aagtttcgga
      721 atcgtttcat ggtttcagga atggttcacg cgcgttatga cttcccatcc attcgcaccc
      781 acgaacccgc ccgcgacgag cagtgacgcc accgtgctga cggccgcgct ggtcgccagc
      841 agcttgcgct tgatgaaaat gtcccaaaac caattcattg ctgtcctgtt ttcaagtgta
      901 gggcgtcggg gcggctcaat cagatgcgat taaattgcca ctttgaccgg cgaccgatgt
      961 tccatagggc taaagcgatt tccgttccag ccggggagcg aagcggccgg gggttgtgga
     1021 aaatagcggg tcttcagcgg
//
LOCUS       DATCBB010000756         1039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_480485, whole genome shotgun sequence.
ACCESSION   DATCBB010000756 DATCBB010000000
VERSION     DATCBB010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480485"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VII37_08355"
     CDS             <1..260
                     /locus_tag="VII37_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY5069787.1"
                     /translation="GGEEKLKIPEGTQSDAVFRVKGKGLPDPRGGGKGDLYYHVRVLT
                     PTKLTREQRKLMDQLGASLKVENKPADRNSSIFEKVKDIFG"
     gene            426..>1039
                     /locus_tag="VII37_08360"
     CDS             426..>1039
                     /locus_tag="VII37_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY5069788.1"
                     /translation="MVLRAQARQLYELSDGTRVFLGRIEKVARDAVEFALLEELPAVE
                     LRLNVRLLLSVVKFDAFEWAIEKATELGVTEIVPLAAARSEKGLIAAAEKRAERWRKI
                     VLESAQQSRRVRLPVVLPVAKPEAAFASRAERLGIFLSERVEAPALRVVLGDRASSHA
                     VLAIGPEGGWTDEERDGALKAGFHEASLGRLILRTETAVIAALAS"
BASE COUNT          207 a          218 c          373 g          241 t
ORIGIN      
        1 tgggcggcga agagaaatta aaaattcccg aaggtacgca gagcgatgcg gtgtttcgcg
       61 taaaaggcaa gggcttgccc gatccgcgcg gcggcggtaa aggcgatctt tattatcacg
      121 tgcgagtgct gacgcctacg aaactgacac gagagcagcg caaattgatg gaccaactgg
      181 gagcttcgtt gaaggtggaa aataagccgg cggaccgaaa ctcgtccatc tttgaaaaag
      241 tgaaagatat tttcggttaa gcgtaacttg tcgtggtggt tcaaattccc caggataaag
      301 ttgttctgat ttccagccgt tctggctatg taggatagaa tcgtcgcgtg cggcggcgat
      361 ttttcgttga aaaattcgag gggcaacggg cgccggtttc gggcgataga gcgcaccatt
      421 tgggagtggt gctgcgcgcg caggccagac agctttatga attgagcgac ggcacgcgtg
      481 tttttcttgg gcgcatcgaa aaggtggcgc gcgatgctgt ggagtttgcg ctgcttgaag
      541 aattgcctgc ggtcgagctg cgactgaacg tccgcttgtt gctttcggta gtgaagttcg
      601 atgcgtttga gtgggccatt gaaaaggcta ccgagttggg ggttacggag attgtgccgc
      661 tggcggcagc gcgcagcgag aagggtttga ttgcggcggc ggaaaagcgg gcggagcgtt
      721 ggcggaaaat tgtgctggag agtgcgcagc agtccaggcg cgtgaggttg cctgtggtgc
      781 tgccagttgc caagcctgag gcggcgttcg catcgcgcgc agagcgcttg ggtatttttc
      841 tttctgagcg ggtggaggcg cctgcgttgc gtgtcgtgtt gggagatcga gcttcatccc
      901 atgcggtgtt ggctatcggg ccagaaggcg gttggacgga tgaggagaga gatggggcat
      961 tgaaggcggg ttttcatgag gcttcgcttg ggcggctgat tttgcggacg gaaacggcgg
     1021 tcattgccgc actggcgtc
//
LOCUS       DATCBB010000757         1359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_155234, whole genome shotgun sequence.
ACCESSION   DATCBB010000757 DATCBB010000000
VERSION     DATCBB010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155234"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VII37_08365"
     CDS             complement(<1..530)
                     /locus_tag="VII37_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5069789.1"
                     /translation="MHCRIQKAVSLAAACALSVFPATSAFAADEPDLSTKTPIKHVVV
                     IFQENVSFDHYFATYPNATNPGAEPQFHAKDDTPRVNNLLAAGLLTENPNSIQPFRLD
                     RSQAVTCDQDHNYGDEQKAFDRGLMDKFPESVGSGTSAGFPCFDAAKGPGIVMGYYDG
                     NTVTAFWNYAQHFAMS"
     gene            765..>1359
                     /locus_tag="VII37_08370"
     CDS             765..>1359
                     /locus_tag="VII37_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5069790.1"
                     /translation="MGVLARKLRFTDYFALAFGTMVGVGWLVVMDDWLGRGGPVGAIL
                     GFAIGGVLLLPVGYVYGQWVQRLPDAAGEAAYTAQVFPPSVSYFTGWVMLLAYFIVCP
                     WEAVAVGKIAAYLFPSLNSFELYRIGGHPVFLPRLLLGLALTVFIAMINYRGVRASAT
                     FQNWSAGSVLVLFLVIVGASAAHGSPANFHPAFSGTPL"
BASE COUNT          252 a          330 c          416 g          361 t
ORIGIN      
        1 tcgctcatgg cgaaatgttg ggcgtaattc cagaatgcag tgacggtatt tccatcgtag
       61 tagcccatca ctattccagg gcccttggct gcgtcgaagc aaggaaagcc tgccgacgtg
      121 ccgctaccta cagattcggg aaatttatcc atcaggccgc gatcgaatgc cttctgctcg
      181 tcgccatagt tgtgatcctg gtcgcaagtt accgcttgag agcggtccag gcgaaaaggc
      241 tgtatggaat tgggattctc tgttagcaga ccagcagcca gcaggttgtt gacacggggc
      301 gtatcatcct tggcatggaa ttgcggttcg gccccgggat ttgttgcgtt cggatacgta
      361 gcaaaataat gatcgaacga aacgttctcc tgaaaaatga ccacgacgtg tttgatgggc
      421 gtcttggttg aaagatccgg ctcgtctgcg gcgaatgcag acgtggcggg gaacacgctg
      481 agggcacacg ctgcggccag cgacacggct ttttggatgc ggcaatgcat atcgactctc
      541 tccttgagtg gctgcgtttt cgatgacgtt aatttgcgaa gattttttag cgagcgcgtt
      601 cgcctgtcag agtgagtctg cacgttgaaa ccagagcact ctgggacgat tccgcgcatg
      661 gtggttctaa tcgacttttg ttattggcgt atgaatgcca cgtcaaaaca aagtttcatg
      721 tggatggtga tgcttacgca cgcagtagcg agatcgctta agtgatgggc gtactagcac
      781 gcaaactgcg tttcaccgat tatttcgcgc tggcgtttgg gaccatggtg ggagttggct
      841 ggctggtcgt gatggacgat tggctgggcc gcggagggcc ggtcggggcg attctagggt
      901 ttgctatcgg cggagttctg ttgttgccgg taggttacgt ttatggccaa tgggtgcagc
      961 ggctgccaga cgctgccggt gaagccgcct acaccgctca ggtttttccg ccgtcggtca
     1021 gctacttcac cgggtgggtg atgctgctcg cgtattttat tgtttgccct tgggaagcag
     1081 tggccgtcgg caaaatcgca gcgtatcttt ttccgtctct caattccttc gagctatatc
     1141 gcattggtgg tcacccggtg ttcctgcccc gactgctgtt gggtttggcg ctgaccgtct
     1201 ttatcgccat gatcaattac cggggcgttc gcgccagcgc aacgtttcaa aattggtcgg
     1261 cgggaagtgt cctggtgctg tttttggtaa ttgtcggggc cagcgcggcg cacggatcgc
     1321 ctgcgaattt tcatccagct tttagtggta cgccgcttg
//
LOCUS       DATCBB010000758         1194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_348907, whole genome shotgun sequence.
ACCESSION   DATCBB010000758 DATCBB010000000
VERSION     DATCBB010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348907"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /gene="mreD"
                     /locus_tag="VII37_08375"
     CDS             complement(<1..450)
                     /gene="mreD"
                     /locus_tag="VII37_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY5069791.1"
                     /translation="MNDTFDIRNMEAHIEVHKFRGGAIVGATILALMFQAFVPVYFEK
                     ASMCDLPLLITIYFGLSRRNPSTGLLLGMMIGLLQDSLSGPTVPLGLYGIAKTFIGYL
                     ASSIGARLDTEHPAARFALTIIFFGLHQGIVLLTRRILLAQSSTWFTM"
     gene            complement(447..>1194)
                     /gene="mreC"
                     /locus_tag="VII37_08380"
     CDS             complement(447..>1194)
                     /gene="mreC"
                     /locus_tag="VII37_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5069792.1"
                     /translation="YFALQNTSRDNEQLRRENDELKMKVDRLQSKAAEADRLALLLNF
                     RKSHENVPLLSARVIATSAGTASEGIVLDRGSKDGIKKNMGVITPEGVVGKVVEVYDD
                     ASEVLLLTDKDSGVGAMLGNSRIQSPVGGAGEPLLVMKYVANDDVVNVGERVVTSGMD
                     KIFPRDLPIGTIVEIKAGNPFKSIRVRPSANLARLEEVIVLLSLQPLQLKQTPEPVKS
                     SSGENGAGAAPANPAASTTADPKTAAVKPQ"
BASE COUNT          239 a          410 c          290 g          255 t
ORIGIN      
        1 catcgtgaac cacgtactcg actgcgccag caaaatccgc cgcgtcagca gcacaatccc
       61 ctggtgcaat ccaaaaaata taatcgtcag cgcaaaccgc gccgcgggat gctcggtatc
      121 caatcgcgcc ccaatcgagg acgccagata cccaataaac gttttcgcga tgccgtataa
      181 ccccagtggc accgtcggtc cgctcaggct gtcctgcagc aatccgatca tcatccccag
      241 caaaagtccc gtcgacggat tgcgccggct caacccaaaa taaatggtga tcagcagcgg
      301 caaatcgcac atcgaagctt tctcgaaata aaccggcacg aaagcctgga acatcaacgc
      361 cagaatcgtc gcgccgacaa tggctccgcc gcgaaacttg tgcacctcaa tgtgcgcttc
      421 catgttgcgg atatcgaaag tgtcgttcat tgcggcttca ccgccgcagt tttggggtca
      481 gccgttgtcg aggccgctgg attcgccggc gccgcgcctg ctccattttc gccgctcgaa
      541 gatttcaccg gctccggcgt ctgcttcagt tgcagcggct gcaggctcag cagcacaatc
      601 acttcttcca gccgcgccaa attcgccgaa ggccgtacgc gaatactttt gaacgggttt
      661 cccgccttga tctcgacaat cgttccaatt ggcagatcgc gcggaaaaat cttgtccatg
      721 ccgctggtca ccacccgctc tcccacgttc accacgtcgt cgttcgccac gtacttcatc
      781 accagcagcg gctcgcccgc gccgcccacg gggctctgga tgcgcgaatt ccccagcatc
      841 gcgcccacgc cgctgtcttt gtcggtcagc agcagcactt cgctggcgtc gtcgtaaact
      901 tccacgactt ttcccaccac gccttccggc gtaatgactc ccatattttt cttgatgcca
      961 tccttcgagc cgcggtccag cacgattcct tcactcgcgg tccctgcgct ggtagcgatc
     1021 acccgcgcgc tcaacagcgg cacgttttca tgcgatttgc gaaagttcag cagcagcgcc
     1081 agccggtcgg cttccgccgc tttgctctgt agccgatcca ccttcatttt cagctcgtcg
     1141 ttctcgcggc gaagctgctc gttatcccgg gaagtattct gcagcgcgaa ataa
//
LOCUS       DATCBB010000759         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_186025, whole genome shotgun sequence.
ACCESSION   DATCBB010000759 DATCBB010000000
VERSION     DATCBB010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186025"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VII37_08385"
     CDS             <1..270
                     /locus_tag="VII37_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069793.1"
                     /translation="NRLVEVAQRSGVRAQLIDSAADIDVSWLEGVARVGLTAGASAPE
                     VLVEQVSQRLAEFGFTDQRDLDLIREDVRFTLPPELATIAAAPRR"
     gene            381..1481
                     /locus_tag="VII37_08390"
     CDS             381..1481
                     /locus_tag="VII37_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY5069794.1"
                     /translation="MAAKITRQPKKIALIGAPSSAAAFLAGSEKAPAALRAAGLVERL
                     QSAGYEVIDHGDCAVRHFAEDDENRRARNLKEIVAGLNDLKLRAEVAVKSGALVLVLG
                     GDCAQVIGLLTGARRYYKRLSLLWFDRDADLNTPATTPSGRIDGMVVAHIIGKGAPEL
                     VRFWGEPPLVREPDVTLFGLERVDAPEQEFLDKSPMRRVSAEVVQAKEAGEAAKSTLM
                     QMHADARQVILHLDTDVIAQEEFPGVNVPGSGGLSFADVRAAFIEFIKNPNLLGLDVA
                     QYNPDKDRDGAGAKKLVDLLVEALTARLQAIPESETTPAATVATPPASSVSATGDVTS
                     PPSQTSEVTTQQNGSTDGPRSEEESSTEMSAD"
     gene            complement(1583..2494)
                     /locus_tag="VII37_08395"
     CDS             complement(1583..2494)
                     /locus_tag="VII37_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5069795.1"
                     /translation="MDSTTVLDLNWMGRPHSIGAVLLESDGHNAILDPGPASTLEALR
                     AQLQLRGLSVADLDAILLTHIHLDHAGASGTLLGENPNLRVFVHSKGAAHLADPSKLL
                     ASAGRLWGDDLQRLFGETLAVPQENIQILDGEETVSVGTRKIEVIYTPGHASHHVTYF
                     DEQEGVAFAGDTAGIRIANGPYIMPATPPPDIDLAVWEQSFAAILDRQPSKLFVTHFG
                     YAGNPAKHIADFRERLHLWAEITERAMQSSSDIAVALQFFLEQTQTEMREYLSEADAE
                     QHAFTAGLPLSFLGLARHIRKRSAAPE"
BASE COUNT          581 a          684 c          770 g          551 t
ORIGIN      
        1 aaccgcctcg tcgaagtagc ccagcgtagc ggcgtccgcg cgcaactgat cgattcggcc
       61 gctgatattg atgtgagctg gctcgagggc gtggctcgcg tgggactcac tgctggggcg
      121 tcagcacccg aagtgctcgt agaacaagtg agccagaggc tggccgagtt tggatttacg
      181 gatcagcgtg acctggattt gatccgcgaa gatgtgcgtt ttacgctgcc gccggagttg
      241 gcgaccatcg cggcagcgcc gcgtcggtaa ttgatttcga agttcgatgt tcgaatcaga
      301 ttagaaactt agggcacagt gtggtttcgc ccgcaattta catagcatca ttcgcggaac
      361 cgggttggta aggagcgaaa ttggcggcga aaatcacgcg gcagccgaag aagattgcct
      421 tgattggcgc accgtcgagt gctgcggcat tcttggccgg cagtgaaaaa gcgcctgcag
      481 ccttgcgtgc agcggggctc gtagagcgcc tgcaatccgc cggatatgaa gtcatcgatc
      541 atggcgactg cgctgttcgc cattttgccg aagatgacga aaatcgccgc gcgcgaaacc
      601 tcaaagaaat cgtcgcagga ttaaacgatt tgaaattgcg cgccgaagtg gccgtgaaga
      661 gcggcgccct ggttctggtt ctgggcggag attgtgcgca agtaatcggc cttctgaccg
      721 gcgcgcgccg atattacaaa cgtctcagct tactgtggtt tgatcgcgat gcagatttaa
      781 atactccggc gactactccc tcgggccgca ttgacggcat ggtcgtggcc cacattattg
      841 gaaaaggcgc gccggaactg gtgcgtttct ggggcgagcc accactggta cgcgagccgg
      901 acgtaacgct cttcggtttg gaacgtgtcg atgcgccaga gcaggagttt ctggataaat
      961 cgccaatgcg aagagtttcg gccgaggtgg tgcaggccaa agaagcgggc gaagccgcca
     1021 agtccacctt gatgcaaatg catgcggatg cgcggcaggt gatcttgcat cttgataccg
     1081 atgtgatcgc gcaggaagag tttccggggg ttaacgttcc gggcagcgga ggattgagtt
     1141 ttgcggatgt ccgagccgct ttcatcgagt ttataaagaa cccgaatttg ttaggcctgg
     1201 atgtggcgca gtataacccg gacaaagatc gtgatggtgc aggagcaaaa aaactcgtgg
     1261 acctgctggt ggaagcgctg actgcaagat tgcaagcgat tccagaaagt gaaacaaccc
     1321 cagccgcaac ggtcgctacc ccgcccgcaa gctccgtctc agcgactggc gatgtgacct
     1381 cgccgccttc acaaacatcg gaagtgacca cgcaacagaa cggctcgacc gacggacctc
     1441 gttctgaaga agaatcctca accgagatgt ctgcggacta aactaacgcc tacttgctgc
     1501 aggcacccat cttcctgcag acgcccatgt gaccgtccgc gcgattcctc taatcctttc
     1561 ctgataacct cgctcagttt cgctattctg gggccgcgct gcgtttgcga atatgccgcg
     1621 caaggcccag gaaagatagc gggagtcctg ctgtaaaggc atgctgctcg gcatcggctt
     1681 cggaaagata ttcccgcatt tctgtttgag tttgctccag gaaaaattgc agtgcgacgg
     1741 cgatgtccga gcttgattgc atagctcgtt cagtaatctc cgcccacaga tgtagccgct
     1801 cccggaagtc agcgatgtgt ttcgcgggat ttcccgcata accgaagtgt gtaacgaaca
     1861 gcttcgatgg ttgacggtcg agaattgccg cgaaggactg ttcccacacc gcaaggtcaa
     1921 tatccggcgg cggagttgcc ggcatgatgt acggaccgtt cgcaatacga attcccgcgg
     1981 tatcgccggc gaaggcgacg ccctcttgct catcaaaata tgtcacatgg tgagaggcat
     2041 ggcccggagt ataaataact tcaatttttc gagtccccac tgaaactgtt tcttcgccgt
     2101 ccaagatttg aatgttttcc tgtggtaccg cgagggtttc gccgaacaga cgctggagat
     2161 catcgcccca aagtcgtccc gcgctcgcga gcagtttaga gggatcggct aaatgcgcgg
     2221 cgcctttgct atgcacgaag acgcgtagat tagggttttc gccaagcaga gttccgcttg
     2281 ctccggcatg gtcgagatga atgtgggtaa gaaggatggc gtcgagatca gcgacggaaa
     2341 gtccacgaag ctgcaattgc gcccgtaagg cctccaaagt ggatgcaggc ccaggatcga
     2401 gaatagcgtt gtgaccatca gattccagaa gaacagcgcc gatcgagtgc ggacgcccca
     2461 tccagttcag atcaagtaca gttgtcgaat ccatatgtga atggtacggg agcgttgcga
     2521 ctggaacaaa ttgccgccag ccatactctt acgccgtccg ccgcatccaa ctgtcgacgg
     2581 cgggag
//
LOCUS       DATCBB010000760         7185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_316214, whole genome shotgun sequence.
ACCESSION   DATCBB010000760 DATCBB010000000
VERSION     DATCBB010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316214"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VII37_08400"
     CDS             <1..1281
                     /locus_tag="VII37_08400"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY5069796.1"
                     /translation="NPRMLLIKNGRVLDPATKTDAGQDILLDGEKIAELAAPGKITPA
                     RDAEIVDAAGLIIAPGFIDMHVHLREPGQESSESIETGTRAAARGGFTAVCCMPNTKP
                     VNDNASVTRFIVDRAKSTGSVRVWPIGAASVGSKGENIAEIAAMKQAGIVAVSDDGKP
                     IATAKLARQVMDYCRSLDLPVIEHAEDVSLAHGAVMREGITSTRLGLRGMPAAAESVC
                     VARDVQLAELTDARLHIAHLSARSSLELVRWAKQRGLKVTCEVTPHHFTLIDEDVQYD
                     SRFKMNPPLAAREDREALLAGLADGTVDAIATDHAPHEPALKDVEFDRAPFGILGFET
                     ALGLALELVHSGKITLMRMVELFTTGPAKVFGMERKIAKGETADLTLFSTGHDWTYNV
                     KESSSKSRNSPFDGRSFKGAPMATIVAGRVVWKR"
     gene            complement(1353..2231)
                     /gene="thiD"
                     /locus_tag="VII37_08405"
     CDS             complement(1353..2231)
                     /gene="thiD"
                     /locus_tag="VII37_08405"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY5069797.1"
                     /translation="MSNHINMAPNLLLTIAGFDPSCGAGIAADLKTFAAHGCYGVAAI
                     TSMTVQSTEGVQAVHNTPSAELREQLEVLAKDCDIAAVKIGMLGNRGNAVVVAEFLDA
                     HKFAHVVHDPVMKSSSGAELLDAAGLKYLTTELLKRSTVITPNVPEAEILTGLEIKDV
                     AGMEAAARKIVEMGAHAVIVKGGHMERAIDVLFDGETITQLGGDRVKANNNHGTGCTF
                     ASAIAAQLASGRGLAEAAMLAKAYVTKAIEKGYAIGKGRIPLDHFYRLRAEPPARGIH
                     EVPQHGMHPAAEPTAH"
     gene            complement(2514..3836)
                     /locus_tag="VII37_08410"
     CDS             complement(2514..3836)
                     /locus_tag="VII37_08410"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY5069798.1"
                     /translation="MATVAKPTAPTRQIGAPPMVPRIAPREDLNPFRISQIQFDMAAE
                     FLKLDPGLRQILRTPKRVLEVSIPTKMDNGQVKVLTGYRVQHNVARGPAKGGVRFHPN
                     LTVDEMKALAAGMTWKTATVNVPFGGAQGGVICDSKRMSKGELERVTRRYASEILPLI
                     GPYLDIPAPDVYTDAQTMAWMMDTYAMTVGHAAHGSVTGKPSSIGGSNGREEAMARGL
                     VAVVEEACKLKKIALRGATIAIQGFGHSGATVARLFAEKKAKIIALSDTRGGVTNSRG
                     IDPIKAFRYKERSGTVVGMPGASRTTNDELLTMKCDILVPAALENVITLNNAEAIKAR
                     IVAEAANGPTTPHADEILARKGITVLPDILANAGGVTVSYFEWVQNQQGLVWDAEEVN
                     ARLQKTIVRAFHEMVETMRKHHVPPRAGAMILAVGRVAEATLVRGLFP"
     gene            complement(3924..4238)
                     /locus_tag="VII37_08415"
     CDS             complement(3924..4238)
                     /locus_tag="VII37_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069799.1"
                     /translation="MNFLARLIRFLFWVVIVSWGVRLAGRLFSGLLQPGQRREPLTPS
                     GDLNAEQGARQLVRDPICGVHIAEVLAIPLRENGELLHFCSLACRDAYLNSSRKLAAN
                     AS"
     gene            complement(4526..5815)
                     /gene="serS"
                     /locus_tag="VII37_08420"
     CDS             complement(4526..5815)
                     /gene="serS"
                     /locus_tag="VII37_08420"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY5069800.1"
                     /translation="MHDLSYFREHLDLFADMAAKRGATLDLDAFRALDKERRELITKN
                     EQRKAQRNKASDEIARLKKEKQPADVIISEMKQVSELIKQSDERISQLDDTQRELLLT
                     IPNIPHASVPVGSSAADNVEVRRWGAPPKFEFVPRPHWEVGERAGILDLSAAAKITGA
                     RFAVYKSWGARLERALANFFLDVHTREHGYTEILPPFLVNSASLLGSGQLPKFAADLF
                     KIEGTDFWLTPTSEVELHNLYRDETVTESQLPIFLTAWTACFRSEAGAAGKDTRGILR
                     QHQFQKVELFKFTHPEKSYEEHEALVRNAEIILQKLGLHYRTVLLCTGDMGFASAKTY
                     DIEVWLPSANEFREISSCSNCEAFQSRRAGIRFKPAGGGKSDFVHTLNGSGLAVGRTW
                     IAIIENYQQEDGSVLVPEVLRPYMGIDRIPAPQTLKG"
     gene            complement(6916..>7185)
                     /locus_tag="VII37_08425"
     CDS             complement(6916..>7185)
                     /locus_tag="VII37_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069801.1"
                     /translation="LTDLTRGLKDYPQIIVNVKVRSKPPLDSLPEVSRALSDANSVLG
                     QSGRIVLRYSGTEPLARVMVEAEHDADVQRFSESLANAVRASIGV"
BASE COUNT         1667 a         1975 c         1966 g         1577 t
ORIGIN      
        1 aatccacgca tgctgctgat taaaaacggg agggtgctgg atcccgcgac gaaaacggat
       61 gccggacagg acattcttct cgatggcgag aaaatcgcgg agcttgccgc gccagggaag
      121 attactccag cgcgagatgc agaaattgtc gacgcggctg gattgatcat tgcaccgggt
      181 tttatcgata tgcatgtgca tttgcgagaa ccgggccagg aatcttccga aagcatcgaa
      241 accggaacgc gagcagcggc gcggggtggg tttaccgcgg tatgttgcat gcccaacaca
      301 aaaccagtga atgacaacgc ctcagtgacg cgatttattg tggatcgcgc caaatcgacc
      361 ggcagcgtgc gggtgtggcc catcggcgcg gcatcggtag gaagcaaagg agaaaacatc
      421 gcggaaattg ccgcgatgaa gcaagctggc atcgttgcag tctccgatga cggcaagccc
      481 atcgccacag caaaattagc gcggcaagtg atggactatt gccggtcgct ggatttacca
      541 gtgatcgaac acgccgagga cgtttctcta gcgcacggcg cggtgatgcg tgaagggatc
      601 acctccactc gactcggttt acgcggaatg cctgccgcgg ctgagtccgt gtgtgtggca
      661 cgagatgttc agctcgcgga attgacggac gcccgcctgc atatcgcgca tctttccgcg
      721 cgaagttcgc tggaactggt gcgctgggcg aaacagcgcg gcctcaaggt cacatgtgag
      781 gtaaccccgc atcacttcac tttgatcgac gaggatgttc aatacgattc gcgattcaaa
      841 atgaatccgc cactggcggc gcgtgaagac cgagaggcgc tgctagctgg actagcagat
      901 ggcaccgtgg atgccatagc caccgatcac gcgcctcacg agccggcctt gaaggatgtg
      961 gagtttgatc gcgcgccttt tggtattttg ggttttgaaa ccgcgctggg ccttgcgctg
     1021 gaactcgtgc acagcgggaa gatcacgctg atgcgcatgg tcgagttatt caccacgggc
     1081 ccggcgaaag tttttggtat ggagcgaaaa atcgccaagg gcgagacggc ggacctgact
     1141 cttttttcca ctggccatga ctggacttat aacgtcaagg aatcatccag caaatcacgc
     1201 aattcgccgt ttgacggccg cagcttcaag ggcgctccaa tggccacgat tgtcgcaggc
     1261 cgcgtcgtgt ggaaacgcta gccgcgaact tccataaaag aactccgatc tcatgcgcga
     1321 aaagatgttc gtcgctgtct tcccaattaa aattagtggg ctgtaggctc agcagccgga
     1381 tgcatgccat gttgcgggac ttcatggata ccacgtgcgg ggggttcggc acgcagccgg
     1441 taaaaatgat ccaaagggat gcggcccttg ccaatcgcgt agcctttttc gatcgctttc
     1501 gtcacatacg ccttggcgag catagccgct tccgccagcc cgcggccgga ggccagctgc
     1561 gcggcaatcg ctgacgcaaa agtgcaaccc gttccatggt tgttgttcgc ctttacccgg
     1621 tcaccaccca attgtgtgat cgtttcgccg tcaaaaagta catcgatggc acgctccatg
     1681 tgaccgcctt ttacgatcac cgcgtgcgcg cccatctcca caatctttct ggcggcagct
     1741 tccatgccgg ctacgtcttt aatctccagt ccagttaaga tttctgcctc gggcacgtta
     1801 ggagtgatca ccgtggagcg cttcagcaat tctgtagtga gatatttgag cccggcggca
     1861 tcgagcagtt ccgctccact ggaagatttc atcaccgggt cgtgaaccac gtgcgcaaat
     1921 ttgtgcgcat ccaggaattc agcgacaacc acggcgtttc cgcgatttcc gagcataccg
     1981 atctttaccg cggcaatgtc gcagtctttg gccaatactt ccaattgttc gcgcaattca
     2041 gcgctcggtg tattgtgcac cgcctgcaca ccttcggtac tctgcaccgt catcgaagta
     2101 atagccgcca ccccgtaaca accgtgagcg gcaaaagttt tcagatcggc cgcgatgccc
     2161 gcgccgcaag aaggatcgaa tccagcgatg gtaagcaata aatttggagc catgtttatg
     2221 tggttagaca tttttcgtgc ttaatcctca gcggacgcta cagcaagccg cactattctc
     2281 agtctcatgg agcgcgatga gcttcaacgt caaaaatccg tgccaccatt ctcttgaagc
     2341 gttcactcca agcgaaaatt gccgggcgtt ttcggagaac caaccaaaaa cgcaccggca
     2401 attgcgaatg tacgcgtgca aaaggtgtga cgcaagaaaa agtttcagaa aaaaacgcag
     2461 agtaggactt ctaacagttg tggtgacgcg cccggattca gaaataattc agtttacggg
     2521 aacaatccgc gcaccagcgt ggcttccgca acgcgtccaa cggccagaat catcgcgccg
     2581 gcgcgtggcg gcacgtgatg cttgcgcatg gtttccacca tttcatgaaa cgcgcgcacg
     2641 atcgtttttt gcaggcgggc atttacttcc tcggcgtccc agaccagccc ttgctggttc
     2701 tgcacccact caaaatagct gactgtcacg cctccggcgt tggcgaggat gtccggcaac
     2761 acggtgattc ccttgcgtgc gagaatttca tcggcgtggg gagtggtcgg cccattagcg
     2821 gcctcggcaa cgatgcgcgc tttgatggct tccgcgttgt tcagggtgat gacgttttcg
     2881 agagcagcgg gcaccaggat gtcgcacttc attgtcagta attcgtcatt ggtagtgcgc
     2941 gaagcacccg gcatgcctac gaccgtgccg ctgcgttcct tataacggaa tgccttgatg
     3001 gggtcgatgc cgcgcgagtt ggttacgccg ccgcgtgtat cgctgagcgc gatgattttg
     3061 gcttttttct cggcaaagag tcgcgctacc gtggctccgg aatggccgaa gccttgaatc
     3121 gcaatggtag ctccccgtag tgcgattttc ttgagcttgc aggcttcttc gaccacggcg
     3181 accaacccgc gggccatcgc ttcttcgcgg ccattggaac caccgatcga cgatggtttt
     3241 ccggtaaccg agccgtgcgc cgcgtgcccc acagtcattg cgtaggtatc catcatccac
     3301 gccatcgtct gcgcatccgt ataaacatca ggagcgggga tatccagata tggcccgatc
     3361 agcggcagaa tttcgctggc gtaacgtcgc gttacgcgtt caagctcccc tttggacatg
     3421 cgtttcgaat cacaaattac gccaccctgc gccccgccaa aaggcacatt cactgtcgcc
     3481 gtcttccagg tcataccggc ggccagcgcc ttcatttcat ccaccgtaag gttgggatga
     3541 aagcgaacgc cgcctttagc gggtccgcgc gccacattgt gctggacgcg atacccggtg
     3601 agaaccttca cctggccgtt gtccattttg gtgggaatgg aaacttccag aacgcgcttg
     3661 ggcgtgcgaa gaatctgccg caggccggga tcaagcttta ggaattccgc ggccatatca
     3721 aattgaatct gcgaaatgcg gaatgggttc aaatcctccc gcggcgcgat gcgtggcacc
     3781 atcggaggcg cgccaatctg tcttgtcggg gctgtgggct ttgcgaccgt ggccatcggc
     3841 tcgggtcctt tcctcaagtc ggttagtttg gagctatatt caaagctttg ggaaaataac
     3901 agctacgatt tgttcacgcc tcgtcatgat gcattcgccg ccagcttccg cgaagaattt
     3961 agataggcat cgcggcaggc taacgaacag aaatgcagca actcaccatt ttcacgcaga
     4021 gggattgcga gtacctccgc gatgtgcaca ccacaaatcg gatcgcgcac cagttggcgt
     4081 gctccttgtt cggcatttaa atcgccgctg ggcgttaatg gctcccgcct ctggcccggt
     4141 tgcagcaaac cagagaataa tcgtcccgcg aggcgaacac cccacgacac aattaccacc
     4201 caaaacaaaa atctgatcaa tcgagcgaga aaattcatag ccaactctgc gaccgcgtcg
     4261 gttcttatct acgactgatg atgatgagcg aactcccatc ggcgtcggcg gaaaagcaac
     4321 gacggcccat agcgaggaat tcaccaaagg acgcggtgaa tcgagtgtat gcaaggtcaa
     4381 aagggcgcgt caaacgaaaa ggtgcaattt cagtaagttg cgaagagcgc ccaagcagcc
     4441 caaagatcac cactactggc gcacagagcc aataagtcat ttattaccca tgacttagga
     4501 cctagcaagg cttcgccgta acgcgctaac ccttaagcgt ctggggtgcc ggaattcggt
     4561 caatgcccat gtaaggtcgc aatacctcag ggactaacac tgacccgtct tcctgttgat
     4621 agttttcgat gatagcgatc caggtgcgcc ctactgcaag ccctgagcca tttaaagtat
     4681 gcacaaaatc gcttttgccg ccgccagcgg gcttaaaacg gatgcccgca cgacgagact
     4741 gaaacgcctc gcaatttgag caggaagaga tttcgcgaaa ttcgttcgca gagggcagcc
     4801 agacttctat gtcataggtt ttcgcggagg cgaaacccat gtctccggtg cagaggagca
     4861 cggttcgata gtgcagccca agtttttgaa gaattatttc ggcgttgcga acgagagcct
     4921 cgtgctcctc gtaacttttt tccggatggg taaatttgaa aagctcaact ttttgaaact
     4981 ggtgctggcg aagaatgcca cgcgtgtctt taccggcggc tcctgcttcg gagcggaaac
     5041 aagcggtcca cgccgtcagg aaaatgggaa gctggctttc cgttaccgtc tcatcgcgat
     5101 aaagattatg gagctcaacc tccgacgtcg gcgtcagcca gaagtcggta ccttctatct
     5161 tgaagagatc ggcagcaaat ttcggtaatt ggccggaacc caggagcgag gcgctattca
     5221 ctaaaaaagg cggcagaatt tcggtatacc catgctcgcg tgtgtgtaca tccaggaaga
     5281 aatttgccag tgcgcgttcc agccgcgctc cccagctctt ataaacggcg aaacgcgccc
     5341 cggtaatttt ggcggcagcg gacaggtcca aaatccccgc acgctcgccg acttcccagt
     5401 gcggacgcgg cacgaattca aatttcggcg gcgcacccca gcggcgcact tccacgttgt
     5461 ccgcggcact ggaacctacc ggcacgctgg catgaggaat attaggaatc gtcagcagca
     5521 actcgcgttg cgtgtcatca agctggctaa tgcgctcatc gctttgcttg atgagttcag
     5581 agacctgttt catctccgaa atgatgacgt cggcaggctg tttctctttt ttaaggcgcg
     5641 cgatttcgtc gctggctttg ttgcgttgcg ctttgcgctg ctcgtttttt gtgatcagct
     5701 cgcggcgctc tttatcgaga gcgcggaaag catccaaatc aagagtggcg ccccgtttgg
     5761 cagccatatc cgcaaaaaga tccaggtgct cacgaaaata acttaggtca tgcatgagaa
     5821 ggagaataac gtatcgcggt aggaactggc gagcacctgg agctatttag gagttccgtg
     5881 ctcgcaaaat gatcaagaaa acatcttcgc gagtgggatc agaggaagaa atttaaagtc
     5941 aggcctggca aaaccgctga atgagagatt gttgttctca actatgggtg gcgacagtac
     6001 gaaggtactc ctgtaacggt acacaagcat ggcccggaaa gtaccgctcc tgatgggacg
     6061 agcgaactat cgaccgatac gctggtggag agcacaatcg caattgtgat cgggagttga
     6121 tcgcggcttc cggagcggtt aaggctgctc ctgtgtgccg ttgtgctgct cgcgagcgca
     6181 gcatgacgac gggaagttaa gaacgacttc ggattacgtt tccctccatc cccacgcccg
     6241 agcggccgga acccccaccg gccgctcacc ttttttgggc cctctttcag agacgatcaa
     6301 cgaatttcac cgctgctaag tgcggcaccc gttctgcgct gatcggatgc ggtcattgaa
     6361 tctgaattcc aacgctaaaa acgacacatg ctcaaattaa ggaatagtgg acctagggca
     6421 ggatctgaag agtccaaagc gagcgctaga agaatggaac ccaaaaaaga aaaaaggggg
     6481 ctggtgcaag cccccttttt gaattgacgc cccaaaaccg gaaaccgagt cgaggttcgg
     6541 tccggccacc ccgggaagcc aatctttttt aaagttgcta acagaagagc aagtgttgtg
     6601 ccagcaagca acgatcacaa tttgcaattc aaagtacttg aaagcacgat agttagctga
     6661 agaaaatgcc tcataaagac tgatgacaac tctgaaagcc tacaaaatct cacagaattt
     6721 caggcgagtt ctatgttgtt tgggagcgga acagactttc ggccccgtga ttatcgataa
     6781 tactcttctc gacgaaactg acaatatcgt tattttgctt ttctgacttc ggccgcacag
     6841 gtcgtgactt caaccgatct atcgcttagc aagactcgcg cacatcctgg ttcgacccac
     6901 ttaacagcga ctggtctaaa cgccgataga ggcgcgcaca gcatttgcga gcgactcgct
     6961 gaagcgttgc acatctgcat cgtgttcggc ttccaccatt actcgtgcga gtggctccgt
     7021 gccggagtaa cgcagaacga tgcggccact ctgccccagc accgagttgg cgtcggacaa
     7081 ggcccgcgag acttccggca gcgagtcgag aggcggtttc gaccgcacct tcacgtttac
     7141 gatgatctgt gggtaatcct tcaatcccct ggtcaagtcc gtgag
//
LOCUS       DATCBB010000761         1041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_250857, whole genome shotgun sequence.
ACCESSION   DATCBB010000761 DATCBB010000000
VERSION     DATCBB010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250857"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VII37_08430"
     CDS             complement(<1..321)
                     /locus_tag="VII37_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069802.1"
                     /translation="MFEDSTFESTGRIRNHSRGWMIAAFAFNGSILLALVLIPLIYPE
                     ALPRQMVSILLEAPPPPPAPEPQRAVKATQAFHGTREMEDLRLTAPSQIPPSIKMIDG
                     PEAAP"
     gene            641..>1041
                     /locus_tag="VII37_08435"
     CDS             641..>1041
                     /locus_tag="VII37_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEY5069803.1"
                     /translation="MKLFRLFIFVVAAIQISSAQEASSGASPNPDATLTYIHAAWDTL
                     TRSMTDCHSLVDTKVTTSPILYMPAELPAPPEVVALKDKCHVEVIPLPRRIEKLGDVR
                     PDELTKEGLLYLPNPYVVPGGRFNEMYGWDS"
BASE COUNT          220 a          298 c          290 g          233 t
ORIGIN      
        1 tggcgccgct tccggtccat caatcatttt gatcgatggc ggaatttgac ttggcgctgt
       61 caggcgcagg tcttccatct ctcgcgttcc gtgaaaggct tgcgttgctt tcactgcccg
      121 ctgcggttcc ggagcaggcg gtggcggtgg cgcttcgagc aaaatcgata ccatctgccg
      181 cggcaatgct tcgggataga tcagcgggat caacaccagc gccagcagaa tcgatccatt
      241 gaatgcgaaa gccgcgatca tccagccgcg gctgtggttg cggattctgc cggtggattc
      301 aaacgtggag tcttcaaaca tggccagcct ccccaaaaga attcagccgc gccggctggc
      361 aaggcggatg gcctttcacg cgcagagggg gcgaaccgct ctgcgcgact gttacgtcgc
      421 ggggagcggc aggctggttc cgacgcgctt tggcatacga accacgcgac gacgacatgt
      481 cgccgtgggt cccggcaatc cgttcatccc aatcggtacc actgtgcttg tgtagacacc
      541 gcgtacgctc gaagtgttcc cgaatcaaat tgttcgggca agatgcaaag gcgttgcgat
      601 acactccaga acgtcgatgg aaagtcacat cggagaacct atgaaattgt ttcgcttgtt
      661 tatttttgta gtagccgcaa tccaaatctc gagtgcgcag gaagcatctt ccggtgcgag
      721 tcctaacccc gatgccacgc tgacttatat ccatgcagcg tgggatacac ttactcgctc
      781 catgaccgat tgccactcgc ttgttgatac caaggtcacc acgagcccga tcttgtatat
      841 gcctgccgaa ctccccgcgc cgcccgaagt ggtcgcgctc aaggataagt gccacgtcga
      901 agtaattcct cttccgcgac gcatcgaaaa gctgggtgac gtccggcccg atgaattaac
      961 caaagagggt ttgctctatc tgcctaatcc ctatgttgtt cccggcggac gattcaacga
     1021 aatgtatggc tgggacagtt a
//
LOCUS       DATCBB010000762         2268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_480611, whole genome shotgun sequence.
ACCESSION   DATCBB010000762 DATCBB010000000
VERSION     DATCBB010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480611"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VII37_08440"
     CDS             <1..524
                     /locus_tag="VII37_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069804.1"
                     /translation="LPEILSKEERSLAIKEMTQLRKQFAKLDMPEAVIRQFAAPPHRP
                     QDCVFALTTQTLSADLQTKITPCQFGGNPDCASCGCIASMGLASIASHKLGGFLPVGA
                     IFKASIAIGQWRTRSRSTAVVPTKSSIPRSPQSAHEPSLPIAIQAKEAFPHMPAETLP
                     VLQQAIEKICVRE"
     gene            complement(560..1321)
                     /locus_tag="VII37_08445"
     CDS             complement(560..1321)
                     /locus_tag="VII37_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069805.1"
                     /translation="MNGQVSAALVVMGAMVLFAGSTADRAGAQTALTTRLNAQGSQDN
                     GQQASPPPQNSDETRRDDARSSDNDRARKDDRRADGPQEGDRVTLPPDTIISVRIADE
                     INSGKNHTGDIFTGIVDPSVLVHDQVVIPRGTEAHVRMAEDKKGGHIHGKAEVRLELV
                     SLVVNGRRLGVESDQEVRGQGAAAAKAKAEAKSGEHLGSASSVAGPAGSAAGPVIAVF
                     SAAKVEMKANSRVEFILAEPFTFSRPPVTDAPQNQ"
     gene            complement(1412..>2268)
                     /locus_tag="VII37_08450"
     CDS             complement(1412..>2268)
                     /locus_tag="VII37_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY5069806.1"
                     /translation="YGRMESCIKLPVGAGLWPAFWMLGTAGKWPVGGELDIMENIPES
                     GGSAAGLGPTKIESTIHGPSTVEKGRFSLTEVFTFPGGQRVDDSTPACHVYGAIWSPF
                     MVQFYVDDWQKPFFIRTADDVPAGGRWVYNAPFYFLLNLAVGGDWPGPPNSSTPSVAE
                     MIVDYLRVYKASRVEVPKMSAAVFKTREGPPSSITLVLNSASETGFVFLACEIKVPGS
                     RCSVDTGNALNNSVVDFRSGPSQGAKVIVTSNNSSHQGDVVAAKTPLMVTAYTVSGEQ
                     SSLSIPIE"
BASE COUNT          504 a          708 c          540 g          516 t
ORIGIN      
        1 aacttcccga aatcctcagc aaagaagaac ggtcgctggc aataaaagaa atgacccagc
       61 tgcgaaaaca gttcgcgaag ctggatatgc ctgaggccgt gattcgtcag ttcgctgcgc
      121 cgccacaccg tccacaagat tgcgtgttcg cgctaactac tcaaacactc tcagccgatc
      181 tgcaaaccaa aattacgcca tgccagttcg gcggaaatcc tgattgcgct tcctgcggat
      241 gtatcgcctc catgggcctg gcgtccatcg cctcccacaa gctaggtggc ttcctgcccg
      301 tcggcgcgat tttcaaagct tccatcgcca tcggtcaatg gcgaacgcga tcaagatcaa
      361 ccgcggtcgt gccaactaaa tcttccattc cgagaagtcc ccagtcagca catgaaccgt
      421 cactgcctat agcgattcaa gccaaggaag catttcccca tatgcctgcg gaaacacttc
      481 cagtgttgca acaggccatc gaaaaaatct gcgttaggga gtaattcgcg aatcggcccc
      541 caatttgctg gtccgcgatt tattgatttt gcggcgcgtc cgtcacaggt ggcctggaaa
      601 aagtaaatgg ctccgccaaa ataaactcca ctcggctgtt ggccttcatt tccaccttgg
      661 ctgcagaaaa caccgcgatc accgggcccg cggcagagcc tgcgggacct gcgacactgc
      721 tcgcactgcc taggtgttcg cccgatttcg cttcggcctt agctttagcc gcagcagcgc
      781 cttgtcccct tacttcctga tcgctctcaa cgcctagcct gcggccattg accaccaagc
      841 tgaccagctc cagtcgaacc tcggctttgc cgtgaatgtg tccgcccttc ttgtcttcgg
      901 ccatccgtac atgcgcttcc gttcctcgcg gaatcaccac ctgatcgtga accaacaccg
      961 atggatccac gatgccggta aaaatatctc cggtatgatt tttaccggaa ttgatttcat
     1021 ccgcgatgcg caccgaaatt atcgtgtctg gcggcagcgt gacacggtcg ccctcttggg
     1081 gaccgtctgc tctgcggtcg tctttccgag ctcgatcgtt gtctgatgat ctcgcatcat
     1141 cgcgccgcgt ttcgtccgaa ttttgcggcg gtgggctcgc ttgctgccca ttatcctgcg
     1201 aaccttgcgc gttcaatcga gtcgttaaag cagtttgcgc gccggcacga tccgccgtgc
     1261 tgcccgcaaa caacaccatt gcgcccatca cgaccaatgc cgccgaaacc tgtccgttca
     1321 tgattccccc tggtggattc ttaaatactt tccgagcggc tgggcttgct atttgttgga
     1381 tgtctttgca ccccgaatcg tgtgcagggg atcactcaat agggatcgag aggctcgact
     1441 gctcgccact aacggtatac gccgtgacca tcaaaggagt ttttgcggcg acaacatcgc
     1501 cttggtgtga agaattatta gacgtaacaa tgactttggc cccttgcgaa gggcccgaac
     1561 ggaaatctac caccgagtta ttcagcgcat taccggtatc cacggaacac ctactacctg
     1621 gaacttttat ctcgcaagct agaaatacaa agccggtttc gcttgcggag ttcagcacaa
     1681 gagtgatgct gctgggcggg ccttctcgtg ttttaaaaac cgccgcgctc atctttggca
     1741 cctccacccg gctcgctttg taaactcgca aataatccac aatcatctct gcaacactcg
     1801 gcgtactgct gttcggcggc cccggccagt cgcccccgac agcaagattc agcagaaaat
     1861 agaaaggtgc gttgtacacc catcgcccgc cagcaggcac atcatcggcg gtacgaataa
     1921 aaaatggctt ctgccaatca tccacataaa actgcaccat gaacggcgac catatcgccc
     1981 cgtagacatg gcaagccggc gtcgagtcat cgacgcgctg tcctcccgga aacgtaaata
     2041 cttccgtcag actaaatcgc cctttctcaa cagtgcttgg cccgtgaatt gtcgattcaa
     2101 ttttagttgg ccccagtcct gccgcggaac cgccggactc aggaatattt tccataatgt
     2161 ctaactcgcc gccaaccggc catttgccag cggtgcccag catccagaac gcaggccaca
     2221 agcccgcgcc gacaggaagt tttatgcacg attccattcg cccgtatc
//
LOCUS       DATCBB010000763         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_57434, whole genome shotgun sequence.
ACCESSION   DATCBB010000763 DATCBB010000000
VERSION     DATCBB010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57434"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VII37_08455"
     CDS             complement(<1..475)
                     /locus_tag="VII37_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY5069807.1"
                     /translation="MSNSSETPLSGIVDPAKAEQELNLIRYKRAFARRNFIKNVGLAG
                     AGIAAGAVIQGCSDNSTSTNPKAQSIPETDVLNFALNLEYLEAEFYSVAVTGNTLSSS
                     VTGGTSTATGGAKVTFSDTRIADIANEIYNDEVLHVKYLRTALGGVAVAEPAINLN"
     gene            complement(637..1593)
                     /locus_tag="VII37_08460"
     CDS             complement(637..1593)
                     /locus_tag="VII37_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069808.1"
                     /translation="MEQLVPELLQDVTSRERKLAVCASAGNLAPADRAEILVLLSTDA
                     DPVVAEQASEALRSQPVEAFVEALKRSEALPAIFEHASQHLVGKPAVAEAMVQNRNCP
                     SRFITPHVPQLSTMSVQALMDSLDRVSDSSELAAALLKSSSATADNKRVLQELLGPET
                     PVDEKALMEALMEAEPDAERRQTLLQRLAKMSVSQRVQFAIKGGSEARRTLIRDSNKV
                     VQRAVLQSPRLTDQEVESFAAMSSLTDEILRLIAGNRAFRKNYTVIRNLMNNSKTPLD
                     VTLHMLPSLNAVDLKKLSNNKNVPETLRTSATKLIRTRQAAK"
     gene            complement(1574..>2112)
                     /locus_tag="VII37_08465"
     CDS             complement(1574..>2112)
                     /locus_tag="VII37_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069809.1"
                     /translation="LLSEYDLARGVQKTEPPAPYQNQLPSPPRWIPALGLLALVLLAY
                     GFFRGGSYAWQHYRVAHPSANSAKPQLPAADSSVSSKAPDKLELSVSTSVRSHVRILA
                     DDQTLLDADVMPGDIHHFTAAQQFQIIAADGAAVHLELNGRPITSRAALGNSSRRPNT
                     IVLGYKDLRPSHDGTARP"
BASE COUNT          446 a          485 c          679 g          502 t
ORIGIN      
        1 cattgagatt gatggcaggt tcagcaacag ctacgccgcc gagagcggtg cgcaggtatt
       61 tcacgtgcag tacttcgtcg ttgtaaattt cgttcgcgat atcagcgata cgagtatcgc
      121 tgaaagtcac tttagcgcca ccggtcgcgg tgctggttcc accagtgacg gaggaactta
      181 aagtgttgcc ggtgacagcc acactgtaaa attcagcttc gagatattct agatttaatg
      241 cgaaattcag aacatccgtt tccggaatgc tctgtgcttt gggattggtg ctggtggaat
      301 tatcggagca accttgtatt acggcacccg cggcgattcc ggcgcccgcg agcccaacat
      361 tttttataaa gttgcgtctc gcgaatgctc gtttgtagcg gatcagattt agttcttgct
      421 ctgctttggc aggatctacg atgccgctca aaggtgtttc gctagaattg ctcattactc
      481 ccccattcaa gatgtgatat gcgaccacga cgctgcggtg cgcctcgtgg tagtgatacg
      541 ggaggaatgg agtggcggat gagcgtttga aacgatttaa gctacagcga acgctgtagt
      601 ttagacaata tttctaaagc ttttaaggtt taatgctcat ttagcggctt ggcgagtgcg
      661 aataagtttc gttgcgctgg tgcgcagcgt ttcgggaaca tttttattgt tcgataattt
      721 tttgagatcg acggcattca gcgacggcaa catgtgcagt gtaacgtcca ggggagtttt
      781 ggaattgttc atgaggttgc ggatcaccgt gtagtttttg cgaaaggcgc gattgccggc
      841 gatgagtcga aggatttcat cggtgaggct ggacatggcc gcaaatgatt ccacttcctg
      901 atcggtgagg cgcggggatt gcagcaccgc gcgctgcaca actttgttgg aatcgcgaat
      961 gagcgtgcgg cgtgcttccg agccgccctt gatcgcgaat tgcacccgtt gggaaacgga
     1021 cattttcgca agccgctgaa gcagggtctg gcggcgctcg gcgtccggct cggcctccat
     1081 gagcgcttcc atgagagctt tttcgtcgac gggagtttcc gggccgagca gttcctgcag
     1141 cacgcgcttg ttatctgcgg tggcggatga ggactttagg agagccgcgg cgagctcgga
     1201 agaatcgctt acgcgatcga gcgaatccat cagtgcttga acgctcatcg tggaaagctg
     1261 cggtacgtgt ggagttataa atctggacgg gcaatttctg ttttgcacca tggcttcggc
     1321 cacggcgggc ttgccgacaa gatgctgaga ggcgtgttca aaaattgcgg gaagggcctc
     1381 gctgcgtttc agggcttcga cgaaggcttc aaccggttgg gagcgtaagg cttcggaagc
     1441 ttgctcggcg acgaccgggt cagcgtcggt ggaaagaagc accaaaattt cagcgcggtc
     1501 agcaggggcg agatttcctg cgctggcaca aaccgcgagt ttgcgctcgc gagaagtgac
     1561 gtcttgcaaa agttcaggga cgagctgttc catcgtgaga gggcctcagg tctttatatc
     1621 caagcacaat tgtattcggt ctgcgggaag aatttccgag tgcggcgcga gaggtaatag
     1681 ggcggccatt gagttccaga tgtacggcag cgccatcggc ggcaatgatt tggaattgtt
     1741 gcgcggcggt gaagtgatga atgtcgccgg gcatgacgtc ggcgtccagg agggtttgat
     1801 cgtcggcgag aatgcgcacg tgcgagcgca cggaggtgga gacgctgagt tcgagtttgt
     1861 cgggtgcctt ggaggaaacc gaggaatcag cagcgggcag ttgcggcttc gcagaattcg
     1921 cggatggatg ggcgacgcga tagtgttgcc aggcgtagga gccgccgcgg aagaatccgt
     1981 acgcaagcaa taccagcgcg agcaagccta atgctgggat ccatctggga ggcgagggaa
     2041 gctgattctg atagggagca ggcggttcgg ttttctggac cccgcgcgcc aggtcatatt
     2101 cggagagcaa at
//
LOCUS       DATCBB010000764         1007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_316318, whole genome shotgun sequence.
ACCESSION   DATCBB010000764 DATCBB010000000
VERSION     DATCBB010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316318"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VII37_08470"
     CDS             <1..645
                     /locus_tag="VII37_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5069810.1"
                     /translation="CANLVNLFEASLRLLHPVMPFITEEIWHAMYGGKPPLKSIALAA
                     YPSADESQIDLAAETEMAILQDLIVSVRNLRAELKVEPKVKVPIEVFAHQPEIRAMLE
                     KNSSAVLQDRPANVEKITFVEKSLANLPGARSTSRFDVHAVYEKKIDVAAERERLKKE
                     LEKIDKQLAGAQARLADPQFTSKAPAHVVEGLRKQVEEQTILRNKIVAQLKELG"
     gene            710..>1007
                     /locus_tag="VII37_08475"
     CDS             710..>1007
                     /locus_tag="VII37_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069811.1"
                     /translation="MDWNSRRITAIIENALTEDRATLDATSYACIDPNQRATATILAK
                     QNCVVAGLGCVSRIFDVYAAFDGAVTSHYEVTSHTSIFDGIGVKPGQQIAVIQHN"
BASE COUNT          241 a          234 c          308 g          224 t
ORIGIN      
        1 tgcgcgaatc tggtgaactt gtttgaggct tcgttgcggc ttctgcatcc ggtgatgccc
       61 tttatcacgg aagaaatatg gcacgcaatg tatggcggca agcctccgct gaaatcaatt
      121 gcgctagccg cctacccatc agcggatgag tcgcagattg atttggccgc ggagacagag
      181 atggcgattc tgcaggattt gattgtcagc gtacgcaatc tgcgggcgga attaaaagtt
      241 gagcccaagg taaaagtgcc gatcgaagta ttcgcgcatc agcccgagat tcgcgccatg
      301 ctggaaaaga attccagcgc ggtgttgcag gaccgtcccg cgaatgtgga gaagattact
      361 ttcgtcgaga agtctctggc gaatttgccg ggggcgcgca gcacttcgcg ctttgatgtg
      421 cacgccgttt acgaaaagaa aattgatgta gccgccgagc gcgagcgtct aaagaaggag
      481 ttggagaaga tcgacaagca attggctggt gcgcaagctc gtcttgcgga tccgcagttt
      541 acaagtaagg ctccggcgca cgtggtggaa gggttgcgca agcaggtgga agaacagacg
      601 atcctgcgga ataagattgt ggcgcagttg aaggaactgg gatagagatt taataagatt
      661 gctgagacgg ggctagcccc gtctctacgg aagattcagc agacgaatta tggactggaa
      721 ttcacgacgc attaccgcaa ttattgagaa cgcgctcacc gaagatcgcg cgacgctcga
      781 tgctaccagc tatgcctgca tcgatccgaa ccagcgcgct acggcgacaa ttctggccaa
      841 acagaattgc gttgttgccg gcctcggatg cgtgtcgcgg attttcgacg tctatgcggc
      901 cttcgacggg gcggttactt cgcattatga ggtgacgagt catacttcaa ttttcgatgg
      961 catcggtgtg aagccggggc agcagattgc ggtgattcag cataatg
//
LOCUS       DATCBB010000765         1083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_219791, whole genome shotgun sequence.
ACCESSION   DATCBB010000765 DATCBB010000000
VERSION     DATCBB010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219791"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VII37_08480"
     CDS             complement(<1..592)
                     /locus_tag="VII37_08480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5069812.1"
                     /translation="MHAFALVIFGGTAFVWVILGLRIAYGGLKLPWLKDFPPITGTTV
                     PRISLLFAARDEEEKLPGALGTLAEIDYPELEIIGVDDRSEDATGSILDEFAASHPRF
                     RAIHVTELPTGWLGKPHALQQAYEASNGEWLLFTDADVRFKVDALRRAMTMAGERKLD
                     HLSLFGDVEMVGFWEKVVVTFFGMAFHMATAPSDVIN"
     gene            647..>1083
                     /locus_tag="VII37_08485"
     CDS             647..>1083
                     /locus_tag="VII37_08485"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5069813.1"
                     /translation="MAIIGAGRVGRALGRRLHELDWKIGAVITRNEASARKAVRFIGS
                     GQARATLSRDVLASRLILISTPDDAVVKVAEELARIGAEELRGKVVLHTSGALNSSAL
                     KAVRDYGACVGSMHPLQSFSGVCIPPLEGKVFAIEGDPVAIRLA"
BASE COUNT          246 a          312 c          296 g          229 t
ORIGIN      
        1 gattaatcac atcgctaggc gccgtggcca tatgaaacgc cattccaaaa aacgtcacca
       61 ccaccttttc ccaaaagccc accatttcca cgtcaccaaa taacgacaga tggtcaagct
      121 tccgctcgcc ggccatcgtc atggcgcgtc gcagcgcatc taccttgaag cgcacatccg
      181 cgtccgtaaa caaaagccat tcgccgttgg acgcttcata agcctgctgc aaagcatgcg
      241 gtttccccag ccaacctgtg ggaagctccg tcacgtgaat cgcgcgaaac ctcggatgcg
      301 acgcggcaaa ctcatcgagg atgcttccgg ttgcatcttc cgagcggtca tcgactccaa
      361 ttatttccag ttcgggataa tcgatttctg caagagtgcc taacgcgccc ggaagttttt
      421 cttcctcatc gcgcgcggca aaaagcagag aaatccgtgg aacagtggtt ccagtgatcg
      481 gtgggaaatc tttgagccag gggagtttta agccgccgta cgcgatgcgc aggcccagaa
      541 tcacccaaac aaaagcggtg cctccaaaaa ttaccagcgc aaacgcatgc atgaattggt
      601 gtcggaagcg gccctcaggt tatcctagca tgacatgcgt ggcgctatgg cgatcatagg
      661 tgcggggcgc gttggtcgcg cgctgggacg gcgtttgcat gaactggatt ggaaaatcgg
      721 cgcggtcata acacgaaacg aagcatccgc gcgcaaggcg gttcgcttca tcggctccgg
      781 acaagcacgc gcgacgctct cgcgtgacgt tctggcttcg cgactgatct tgatatccac
      841 tcccgatgat gcggttgtaa aagttgcaga ggaattggca cgaatcggcg cggaagaatt
      901 gcgtggcaaa gtcgtgttgc acaccagcgg cgctttgaat tccagcgcgc ttaaggccgt
      961 ccgggattat ggcgcgtgcg tcggctccat gcatccgttg caatccttca gcggggtctg
     1021 tatccctccg ctcgaaggca aagtttttgc catcgaaggc gatcctgtag ccattcgtct
     1081 ggc
//
LOCUS       DATCBB010000766         1299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_316362, whole genome shotgun sequence.
ACCESSION   DATCBB010000766 DATCBB010000000
VERSION     DATCBB010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316362"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..>1299)
                     /locus_tag="VII37_08490"
     CDS             complement(218..>1299)
                     /locus_tag="VII37_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069814.1"
                     /translation="GFSISVAVVQSEVEFERQLRLYLPDIVLADYNLPQWEGMAAVEV
                     LRREGLDIPVILVSGALGDITAVECIKRGATDYVLKDGLARLPEAIRRALQEKRVLRL
                     RRRAEEDLARKAEELTRSNADLEQFAYIASHDLQEPLRMVAAYTQLIAERYRGKLDEN
                     ADKFIGYACEGALRMQTLIQDLLAFSRVGRAGATAGPVNCDSAIAEVLLTLGPAIRES
                     GATVTHTALPVVWADSSQMTQVFQNLIGNAIKFRGTEAPSVRVTAEQTGQDWLFTVSD
                     NGIGIAPECAENIFVVFQRLHTRTEYSGNGIGLAICKKIVERNGGKIWVEAAVGRGCT
                     FKFTLPCHPAAEKEVCAEEMVAPLV"
BASE COUNT          287 a          400 c          328 g          284 t
ORIGIN      
        1 aattatgcgg aacccgcatt cttcaagaac gccggtcagc aatcgacttg attttggatt
       61 gtcgtccacg taaagcactg tcgttcggtt ctccatcaga aacctccgtt ccttttctga
      121 agaacgcgag cgccggaccg ctcttgcggc tgaagtaggt gaagatttgt aaacgcctga
      181 gatgttggga ataatgatgg tcaaacgaga gttggaacta tacgagaggc gcgaccattt
      241 cttcggcaca cacttctttt tccgcggccg gatggcacgg taaagtgaat ttgaatgtgc
      301 agccgcgccc gactgctgcc tccacccata tcttcccgcc attgcgctcg acaattttct
      361 tgcagatggc caacccgatt ccattgcccg aatattcggt tcgggtgtgc aggcgttgaa
      421 agacaacaaa gatattttcg gcgcactccg gcgcaatgcc gatcccgtta tcacttaccg
      481 tgaacaacca atcctggcca gtttgctcag ccgtcacgcg gactgagggc gcttccgtcc
      541 cgcgaaactt gattgcattc ccaatcagat tctggaagac ctgggtcatt tgcgacgaat
      601 cagcccacac gaccggcaaa gcagtgtgcg tgaccgtcgc tccactctcg cgaattgccg
      661 gacccagtgt gagcaggacc tcggcgatgg cggagtcgca attgaccggt ccggccgtcg
      721 cccctgcccg acctactcgc gagaaggcca gcagatcctg aatcagagtc tgcatgcgca
      781 atgctccctc gcaggcgtag ccaatgaatt tgtcggcatt ctcgtcgagc tttccccgat
      841 agcgctccgc gatcaattgc gtgtacgcgg caaccatgcg cagcggttcc tgcaggtcat
      901 gcgaggctat atatgcaaat tgctcaagat cggcattgga gcgggtcaac tcttccgcct
      961 tgcgcgccaa atcctcttcc gctcgccggc gtaaacgaag cacgcgtttt tcctgcaacg
     1021 cccgccggat tgcttccgga agccgcgcca acccatcctt cagaacgtaa tcagttgcgc
     1081 cgcgtttaat gcattcaact gcagtgatgt ctcccaatgc acccgaaacc agaatgaccg
     1141 gaatgtccag gccttcgcgc cgcaggactt ccaccgctgc catgccctcc cactgcggca
     1201 ggttgtaatc cgccaataca atgtcgggaa ggtacaagcg cagctgccgc tcaaactcaa
     1261 cttccgattg caccaccgca actgaaatcg agaaaccgc
//
LOCUS       DATCBB010000767         1094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_57490, whole genome shotgun sequence.
ACCESSION   DATCBB010000767 DATCBB010000000
VERSION     DATCBB010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57490"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VII37_08495"
     CDS             complement(<1..659)
                     /locus_tag="VII37_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY5069815.1"
                     /translation="MARLESVQVPAGACIPNSTSAAPAATGTSRHSALVRVTHWITTL
                     CFFALLVSGIEILISHPRFYWGETGNVLTTPLFKVPVPASRNLVPTGYGYVLPDQNGW
                     SRYLHFQAAWLVVLTGLLYTISILFTGHLRRSLLPGGDDLSWKVLASEIGNHLRFARP
                     SAAEAWSYNVLQRLAYLLVIFVAFPLVIWTGLAMSPAFVSAVPAGACIPNSTSAAPAA
                     TG"
     gene            complement(879..>1094)
                     /locus_tag="VII37_08500"
     CDS             complement(879..>1094)
                     /locus_tag="VII37_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069816.1"
                     /translation="NEILVEEQDPWNQEQEVNVAMIFHIPGPLREFTSGKSVVKIENS
                     AATLAEALTAFWTLYPGVRDRIVTEQG"
BASE COUNT          276 a          355 c          253 g          210 t
ORIGIN      
        1 cccgtcgcag ctggcgcggc tgaagtagaa ttcgggatgc aagctcccgc cggcaccgcc
       61 gaaacaaacg ccggcgacat cgccaacccc gtccagatca ccagcggaaa cgccacgaaa
      121 atcaccaaca agtaagccag ccgctgcaac acgttatacg accacgcttc cgccgcactt
      181 ggccgcgcaa atcgcaaatg attcccaatt tcactcgcca acactttcca ggatagatcg
      241 tcgccacccg gcagcaagct cctccgcaaa tgccccgtaa acaaaatgga gatggtatac
      301 agcaagccgg tcaacaccac caaccacgcc gcctgaaagt gtaggtaccg gctccatcca
      361 ttctgatccg gcaacacata cccgtaaccc gtcggcacca aattcctcga cgccggtacc
      421 ggcaccttaa acaacggcgt cgtcaacaca ttcccggtct cgccccaata aaatcgcgga
      481 tgagagatca gaatttcaat cccgctaaca agcagagcaa aaaagcacag cgtggtaatc
      541 cagtgggtaa cgcgcaccag cgcagagtgc cgcgaagtac ccgtcgcagc tggcgcggct
      601 gaagtagaat tcgggatgca agctcccgcc ggcacctgaa cagattccaa tctcgccatt
      661 tttagtttcc tactgaggct caagccttgc ttttacgcgt gatactacca cgccgcagcg
      721 actgaaacgt caaccgctgg attattgcgg caggtctctc agccgccgct aacggcgggc
      781 acgatcgaaa tctcagatcc gctcgacacc cgactcgcaa ggccacccgt atatcggacg
      841 ttttcgtcgc cgatgaaaat gttgatgtgt tctcgcactt acccttgctc cgttacgata
      901 cgatctcgaa cgcccggata gagtgtccag aaagccgtca acgcctccgc caaagtagca
      961 gccgagtttt cgattttgac tacgctcttt ccagaagtaa attcgcgcag aggacccgga
     1021 atgtgaaaga tcatcgccac attcacctct tgctcttggt tccacgggtc ttgctcttcg
     1081 actaggattt cgtt
//
LOCUS       DATCBB010000768         1034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_122732, whole genome shotgun sequence.
ACCESSION   DATCBB010000768 DATCBB010000000
VERSION     DATCBB010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122732"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..>1034)
                     /locus_tag="VII37_08505"
     CDS             complement(44..>1034)
                     /locus_tag="VII37_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5069817.1"
                     /translation="LNGEIDIQSTTGLGTTFTLKVPLTLIISPALFVRCGTTSFALPL
                     AVVEEIRRLRSDEIENVGGKLLTKVRDVVTEVVRLDGYLGLPPLEAINGYFHMVVANT
                     GNRQIGLVVEEVLGKDEIVIKNLGEYLRRVKLFPGTTIAPDGSLILLLDLNRMVATDP
                     TERRTLQASASAARIFAPGSAAVAHGTIPAEAIDRVMHERLIVVADDSISVRKFVGRM
                     LEKNGYVVKLAADGLEAAELVTQHGCHLVITDLEMPRMTGYELMVQLRQNASTRRIPV
                     MVVTSRAGAKHRDRAMKEGAAAFLTKPVQEDQLLVAVEQLMGRETERHLVPVS"
BASE COUNT          225 a          304 c          281 g          224 t
ORIGIN      
        1 tcaacacgcg aatctttttg agggcggtgg cggccatgat cgtttaacta accggaacga
       61 ggtgccgttc cgtttccctc cccatgagct gttccaccgc tacaagcagt tgatcttcct
      121 gaaccggctt ggtgagaaaa gcggccgcgc cttccttcat ggcgcgatct cgatgcttgg
      181 cgcccgcgcg cgaagtcacc accatcacag gaattcggcg agtgctggcg ttctggcgca
      241 actgcaccat cagttcgtaa cccgtcatcc gtggcatttc taggtcggtg atgaccaagt
      301 ggcaaccgtg ttgcgtcaca agctccgctg cttccagtcc atcggctgcc agtttcacga
      361 catagccgtt cttttccaac atgcggccga caaacttgcg cacactgatg gaatcgtcgg
      421 ccaccacgat gagccgctcg tgcattacac gatcgatagc ctcagcggga attgtgccgt
      481 gagccaccgc tgcggagccc ggcgcaaata tgcgcgcagc actggccgat gcttgcagag
      541 tccggcgctc ggtcggatcg gtggcgacca tgcgattcaa atccagcaaa agaatcaagc
      601 tgccgtcagg agcgatggtc gtgcccggga aaagttttac gcggcgcaga tattcgccca
      661 gatttttgat gacaatttca tctttgccga ggacttcttc caccaccaaa ccgatctgtc
      721 gattgccggt gttggctacc accatatgga aataaccgtt gatggcttcc agcggtggca
      781 accccagata gccatcgagg cgcacaactt cagtaaccac atcgcgaact ttggtgagca
      841 gcttgccgcc cacattttcg atctcgtcgg agcgcaagcg ccgaatttcc tcgaccactg
      901 ccaaaggcaa ggcaaagcta gtcgtaccgc accgcacgaa tagcgcgggc gagatgatca
      961 gggtcagagg aactttcaga gtgaaggtgg tgcccaatcc agtggtgctt tgaatatcga
     1021 tttcgccatt gagg
//
LOCUS       DATCBB010000769         1150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_219865, whole genome shotgun sequence.
ACCESSION   DATCBB010000769 DATCBB010000000
VERSION     DATCBB010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219865"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..>1150
                     /locus_tag="VII37_08510"
     CDS             225..>1150
                     /locus_tag="VII37_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5069818.1"
                     /translation="MSARLPLLGKTPFVLDPRPLAEATSARAGLLSVSRAYRSLGVPG
                     MVDANLPLRKRQRGFSEAQLIESLVLLQVAGGECPEDMGLLANDACLERGLGYQPPKA
                     TAAREFLERFHDKDLEKLRPAREDQKSFIFPSSGPVAALQEVQAGCARQIAKRYEEQG
                     QKQTIATIDQDATIIESHKRAALYHYEGGRGYQPMVAVWAELDLVVADEFRDGNVPAR
                     QAPLACAKMAFAALPASVRQRYFRGDSACHESELLDWLKHPDRAQEPGGRIGFAVSAV
                     MSQALGEAMKKVKEAEWKTFDTEPEGTLRQWA"
BASE COUNT          237 a          308 c          375 g          230 t
ORIGIN      
        1 tgaatatcca ccgccgtatg aaatgtcccg cgatcctttg ttccggcaat tgacatgcgg
       61 cctttttagg cccaacccgc gccgcaacgc aagcgccatc gcgtttttgg gcaaagccct
      121 aatcctcgcc taattttcgg caattcgcaa aaaagtgcgt aaataccggc tgaaaacaca
      181 ttttttcgtt gacttataca tatagatatt ctatatgtca ttggatgtcc gcgcgcttgc
      241 ccttgctcgg caaaactcca tttgtcctgg atccacgccc tctggcggag gcgaccagcg
      301 cgcgcgccgg gctgctgtcg gtttctcgcg cttatcggtc tctgggggtg ccggggatgg
      361 tcgacgccaa cctgcccttg cgcaaacggc aacgcggatt cagcgaagcg cagttgattg
      421 agtcgctggt gctgctccag gtcgccggag gcgaatgccc cgaagacatg gggcttttgg
      481 ccaacgatgc ctgcctggaa cgaggcctgg gctaccaacc gcccaaggcg acggcggcgc
      541 gggagtttct ggagaggttc cacgacaaag atttggagaa gttgcggccc gcgcgggaag
      601 atcaaaaaag ttttatcttt ccttccagcg ggccggtggc ggcgctgcaa gaagtccagg
      661 ccggctgtgc gcggcaaatc gccaaacgct atgaggagca gggacagaag caaaccatcg
      721 cgaccattga ccaggacgcc accatcatcg agagccacaa gcgggccgcc ttatatcatt
      781 atgagggggg acgggggtat caaccgatgg tggcggtgtg ggcggaactg gatttggtgg
      841 tggccgatga atttcgggac ggcaacgtgc ccgccaggca ggcgccgctg gcctgcgcca
      901 agatggcttt tgcggcgttg ccggccagtg tgcgccagcg ttattttcga ggagattcgg
      961 cctgccatga gagtgagttg ctggattggt tgaagcaccc ggatcgggcc caggagccgg
     1021 gaggccggat tggatttgcc gtcagcgcgg tgatgagcca ggccctgggt gaagcgatga
     1081 agaaggtcaa ggaagcggag tggaaaactt ttgacaccga gccggagggg actctgaggc
     1141 aatgggcgga
//
LOCUS       DATCBB010000770         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_24955, whole genome shotgun sequence.
ACCESSION   DATCBB010000770 DATCBB010000000
VERSION     DATCBB010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24955"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VII37_08515"
     CDS             <1..394
                     /locus_tag="VII37_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069819.1"
                     /translation="GLTHTEVPGARTVNEFFAGLMQGRGRIHGLHGGYAKLTADVFSI
                     VNSLVHDKPWTLAISPLALLVPFFTAGHWLNEIRFSRKWSALLEDSEKRMQRLWDIAP
                     HAHDLIPEHFSFGVSLESSLGSSLGASA"
     gene            404..955
                     /locus_tag="VII37_08520"
     CDS             404..955
                     /locus_tag="VII37_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5069820.1"
                     /translation="MTVTRMLWGFIENRDHRVMRQMNRWHAPRWFRIWMILSTRLGDG
                     WLWYGLGIMLLAWGGPQRLAAIGAAGSAALVGVLIFKALKHLSHRVRPCQFEPHCWSK
                     VLPPDKFSFPSGHTMTAFSIALVLSYFYPALEGTLFFLAISIGFSRIVLGMHFLSDVL
                     AGVVLGVALGCASITAFASLGWI"
     gene            1119..2075
                     /locus_tag="VII37_08525"
     CDS             1119..2075
                     /locus_tag="VII37_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5069821.1"
                     /translation="MVYPGRFKRYSLAAFACLISLPAIAFAEQHAVVGATASVSDRNA
                     ENVSTGINPDYALPDYPAPENSLSESSSSNAAGPASFPLPAASKEDPGEDREASLRKL
                     PMNFLHDQKDMWLFPLELGKGRHWLPALVITGGTAVFIATDPQTMPHFRQTTDFHGFN
                     QVFSTTATGAAIAVVPAVFYGVSLLRHDSYDQGSALFAGEAVADDAILMVVMKAITRR
                     ARPTEFPVTGPYNDTFFHSTSSFFGKGTSFPSGHAMMAFSVATVFARRYKDHRWVPYL
                     AYAVASAIAFSRVTTGAHFPADVFVGSALGFVIARYDVLHGQ"
BASE COUNT          419 a          631 c          576 g          529 t
ORIGIN      
        1 gggcttgact cacaccgagg taccaggcgc gcgcaccgtc aacgaatttt tcgcaggcct
       61 tatgcaagga cgcggccgca tacacggtct gcacggtggt tacgcgaagc tcaccgcgga
      121 cgtcttcagc atcgtcaatt ccctggtaca cgataagcct tggacgctgg caatttcgcc
      181 cctggctcta ctcgtgccgt tctttaccgc cgggcactgg ctaaatgaaa ttcgtttctc
      241 tcgaaaatgg tccgcgctgc tcgaagattc ggaaaaacgc atgcaacgct tgtgggacat
      301 tgcgccacat gcgcacgatt tgattcccga gcatttttcg tttggcgttt cgttagaatc
      361 ttctctcggg tcctcgttag gcgcttccgc atgaggtgag gccgtgactg tgacgcgcat
      421 gctgtgggga ttcatcgaaa accgcgacca tcgggtcatg cggcagatga accggtggca
      481 tgcgccccgc tggtttcgca tctggatgat tctctccacg cgcctcggtg acggctggct
      541 gtggtatgga ctgggaatca tgctgctggc ttggggagga ccgcaacggc tggccgcgat
      601 cggcgcagcg ggttcagcag cccttgtcgg cgtactgatt ttcaaggccc tcaaacattt
      661 aagccatcgt gtaaggccgt gtcaattcga gccgcactgc tggtcaaaag ttttaccacc
      721 ggataaattt tcttttccct cggggcacac catgacggcg ttttccattg cgctggttct
      781 gagttatttt tatccggcgc tggaaggcac gctgttcttt ttagctatca gcattggttt
      841 ctcgcgcatc gtgctgggca tgcatttcct cagcgacgtg cttgccggcg tggtactcgg
      901 agtagcgctg ggctgcgcat ccatcaccgc atttgcgtcc ctgggttgga tataagaagt
      961 acaaggttac tttccaagct aaataaccgt taatgtttac cccgcattat aagttccact
     1021 gctaaagtaa tctgtgtaga gtccccctcc gccgctcgcg ttattcttcg cataaagcgc
     1081 atcccgacta gcgcatccga tcacaggaga ttttttcgat ggtctatcca ggccgcttca
     1141 aaagatatag ccttgcggca ttcgcgtgtc tcatctcgtt gccggccatc gccttcgctg
     1201 aacaacacgc tgttgtcggc gccaccgctt ccgtttccga tcgcaacgca gaaaatgtaa
     1261 gcaccgggat aaatcccgac tatgcgttgc cggattaccc ggctcccgaa aattccttat
     1321 ctgaatcttc gtcatccaac gccgcgggcc cagcgagctt tccgcttcca gcagcctcga
     1381 aagaagatcc tggcgaagac cgcgaagcgt cattgcgcaa gttgccgatg aattttctcc
     1441 acgaccaaaa ggatatgtgg ctgtttccgt tggaacttgg caaaggacgc cactggctcc
     1501 cggctttagt tattactggc ggcaccgcgg tcttcatcgc tacggacccg cagacgatgc
     1561 cgcacttccg ccaaaccacg gatttccacg gtttcaatca agtgttcagc accacagcga
     1621 caggcgcggc aattgcggtt gtgccggccg ttttttatgg tgtgagcctg cttcggcacg
     1681 attcgtatga tcagggctcc gcgctattcg ccggcgaagc agtagccgat gacgcgattc
     1741 tcatggtcgt gatgaaagcc atcacgcggc gcgcacgtcc gacggagttc ccggtgacgg
     1801 gaccttacaa cgacaccttc ttccacagca catcctcatt ctttggcaaa ggcacaagct
     1861 ttccctcagg ccacgctatg atggccttct cggtagccac ggtttttgcg cgacgctata
     1921 aggaccatcg ctgggttccg tatctggcat acgcagtcgc tagcgccatc gccttctccc
     1981 gcgtaaccac cggcgcgcac ttccctgctg acgtcttcgt gggctcagct ctaggattcg
     2041 tgatcgcacg gtatgatgtt cttcacggtc agtgaagaaa aaactcctgc tggaaatttc
     2101 ggttgaatcg atcaatgccg cggcggccgc tgagcgcggc ggcgccgatc gagtt
//
LOCUS       DATCBB010000771         1862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_445788, whole genome shotgun sequence.
ACCESSION   DATCBB010000771 DATCBB010000000
VERSION     DATCBB010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445788"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VII37_08530"
     CDS             complement(<1..761)
                     /locus_tag="VII37_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5069822.1"
                     /translation="MPTIDFHNHFYPPEYIKALEQGPSAVRITHDSAGNPCLHYPGDY
                     NVAVPGHRDIEYREQILKDAKVDTQVITLTTPGTHIEGSTTAIRLARLVNDAFARIVA
                     SKRGRFASLATLPLCDPAASVVELRRAMEQLKFPGVMVFSNVNGTALSHQSFWPLWEA
                     ANELGAVVHIHPTNPLGVEAMLDYWLMPLVGFLMDTTLAAASLVFSGVAERFPNIKWV
                     LGHLGGAIPYLAERLDRGYEAFADCRKNISRPPTS"
     gene            complement(787..>1862)
                     /locus_tag="VII37_08535"
     CDS             complement(787..>1862)
                     /locus_tag="VII37_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5069823.1"
                     /translation="FLRQIYSPALAFLWGWGMFFVMHAGILAAIAVVLARYAGYFFGL
                     GDAEIRVLAIVAILLVSFIQYLGVKPGSAVQVTLTAAKLCAIAVMCALIFILGGHAHH
                     AVPVSAEQPVRLTSFGLAVAAGLFAFGGWHMVSYSAGETRDPERTIPRALIYGVLIVT
                     ACYTLLNAAYLYVMPLSAVATSTRVAADAMERVLSPAGGKAITVVIIVSALGALNGIA
                     LAGPRVYYAMAQDRLAFRWMAALHPKRQTPYLAIAAQAVWACALAATNSYRALFTRVI
                     YTEWLFFALLAAGIFVLHRRGQYTPKYLAFGYPLLPIVSLVTSAAIACNQIYAAPRDS
                     FIGLGLLLLGLPVYFFWAHRNTT"
BASE COUNT          455 a          483 c          590 g          334 t
ORIGIN      
        1 taagaggtgg gaggacgaga gatgtttttg cgacagtcgg caaaagcttc atagccgcgg
       61 tcaagacgct cagccagata agggatggcg ccgccgaggt gccccaggac ccatttgatg
      121 ttgggaaaac gctctgccac gccgctgaag acgaggctgg ctgcggcaag cgtggtgtcc
      181 atgaggaagc caacgagcgg catgagccag tagtcaagca tggcttcaac gccaagcgga
      241 tttgtgggat gaatatggac gacagcaccg agttcattcg ctgcttccca gagcggccag
      301 aaactctgat gactgagggc tgtgccgttg acgttgctga agaccattac gccgggaaac
      361 ttgagttgct ccatggctcg gcgcagctca acgaccgacg cggcagggtc acagagcggc
      421 aacgtggcga gcgaagcgaa gcggccacgt ttggaggcga cgatgcgggc aaaggcgtca
      481 ttgacgagtc gggcaagacg aatggcggtg gtcgatcctt ctatgtgcgt gccgggcgtg
      541 gtgagagtga tgacctgagt atcgaccttg gcgtctttaa gaatttgctc gcggtattcg
      601 atgtcgcgat ggccagggac ggcaacgttg tagtcgccgg gataatgcag gcaaggattt
      661 ccggcggaat catgagtgat ccgaaccgcg ctgggacctt gttctaaagc cttgatgtat
      721 tccggtggat aaaaatgatt atgaaaatcg atcgttggca ttcgtgctcg gtcctctggc
      781 tgcgtgctaa gttgtgttgc gatgggccca gaaaaaatac actggcaacc ccagtagaag
      841 caagcctaga ccaatgaaac tatcacgcgg ggcagcgtaa atctggttgc acgcaatggc
      901 agccgaggtg acaagagata ctatcggcag taggggataa ccaaaggcga gatatttagg
      961 cgtgtactgg ccgcggcgat gcagaacgaa aatccccgcg gccagcagcg cgaaaaacag
     1021 ccattcggta tagattacgc gggtaaaaag cgcgcgatag gaatttgtgg ctgcaagagc
     1081 gcacgcccac acggcttgtg cggcgatcgc gagataagga gtctggcgtt tgggatgaag
     1141 cgcggccatc catcggaagg cgagtcggtc ttgcgccatg gcgtaataaa ctcgaggtcc
     1201 ggccagcgcg atcccgttga gcgcaccgag cgcggaaaca attatcacca cggtgattgc
     1261 cttgccgcct gcagggctga gaacgcgctc cattgcgtcg gcggcgacgc gcgtggaagt
     1321 tgccacggcg ctcagcggca ttacataaag atacgcggcg tttaagagcg tgtagcaggc
     1381 cgtcacaatt aaaacgccat agatgagagc gcgagggatg gtgcgctccg gatctcgcgt
     1441 ctctccggcg gaatatgaaa ccatgtgcca gcctccgaac gcgaatagtc ccgcggccac
     1501 cgccaggccg aaggacgtca ggcggactgg ctgttcggcg gaaacaggca cggcgtggtg
     1561 cgcgtgtcct ccaagtatga agatcagggc gcacatcacg gcgatcgcgc aaagctttgc
     1621 cgcagtgagc gtcacctgca cggcgctccc cggctttacg ccgagatatt gaataaaaga
     1681 aacaagaagg atcgccacta tggccagcac tcgaatttcg gcgtcgccca ggccgaagaa
     1741 atatcccgcg tagcgcgcca ggactaccgc aatagccgct aaaattcctg cgtgcatcac
     1801 aaaaaacatc ccccaccccc acagaaatgc cagggcggga gaataaattt ggcggagaaa
     1861 ca
//
LOCUS       DATCBB010000772         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_122821, whole genome shotgun sequence.
ACCESSION   DATCBB010000772 DATCBB010000000
VERSION     DATCBB010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122821"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VII37_08540"
     CDS             <1..282
                     /locus_tag="VII37_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY5069824.1"
                     /translation="DIPTTDEAGLPGFYFTFWHAFWVPKGTPKEVVAKLNGALRTTLA
                     DPEIRKKLTALAQDIFPPEEQSPEALAKFQREEIDKWWPIIRAANIKAE"
     gene            complement(807..1187)
                     /locus_tag="VII37_08545"
     CDS             complement(807..1187)
                     /locus_tag="VII37_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069825.1"
                     /translation="MTDMLIAAFITLGIAVSIGSGLAIQYLHTEGTKPPPWPLALLHA
                     LFALGGLGCLFLALRGPPRGLDRGTGSFVIIAATLIVMAALVGSLMLAARILKKRPAG
                     ILVGVHATLAVGGFVILVVYVFPG"
     gene            complement(1343..>2279)
                     /locus_tag="VII37_08550"
     CDS             complement(1343..>2279)
                     /locus_tag="VII37_08550"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5069826.1"
                     /translation="LNAHTFAVTETGRTDRVLNTQDVFHAALVPAMIFIMLCLALAWR
                     AGRNQPLLPGERKRLAPQEAILATSALIALLVLLGGVATGYFYAVEAAAVGAFILLCA
                     GFLTGRLHHKVLRELLHDAITITGALFSLLVAATTFTLVLRLLGTDRLVNNLVAGIPG
                     GDIPATAVVLGVIGICAFVLDAFEIIFVIIPIVIPPLLVRVADARWVSVLVLLTLQSS
                     FLLPPFGYALMMVRGAVKNPAPFRPFVRALAPFLVAQWVLLVVVLTVPKLVHFGENIA
                     NNSRAPASPVSNDEIEKRLRDMLPMPPPPLTFDQN"
BASE COUNT          543 a          616 c          695 g          425 t
ORIGIN      
        1 gacattccga caactgacga ggcgggcttg ccgggattct actttacgtt ctggcacgcc
       61 ttttgggtgc cgaagggtac gcccaaggaa gtcgtcgcca agctcaatgg cgcgttgcgc
      121 acaacgctgg ccgaccccga aatccggaaa aagctcaccg cgctcgccca ggatatcttc
      181 ccgccagagg agcaaagccc agaggcgctg gctaaatttc aaagagagga aatcgacaag
      241 tggtggccga tcatcagggc ggcgaatatt aaagcggagt aaaatgcagg ctgacctccg
      301 gttctggcac ttggcgtctt tcgtggtgcc gcaccatttt agtcgctttc ggcggatagc
      361 ggacattgtt cgggattggc gcgagatggc tcagtcgcga atgaccattt cctgtcattg
      421 tgcgtgttaa agagcgagaa tggcgtcatc gccgacctgg gcgggcgaca aggaggcggc
      481 atccatttgc cggggttctt tttgcaaaaa aaaccgcgcc ggatggggcg cgggtttgtg
      541 cagtaatgga ggattcatgc cgctcggaca atggctatgg tcctatgcga cataagcgca
      601 caatcataca cagcgctgct gaagataaac tcgcaagtaa ctttttcata aacgttggcg
      661 cggcggaaat gtattccgac gctcggcatg ggtcgaaaat atttaaccgc cttttattca
      721 ccagtgctgg ttaagagcgg ccccaacgtc acgcgcttta gtagtggcaa gagtatggtg
      781 cgctcgctga agcgggagtc ttccagctat cctggaaaaa cataaacaac gagaatgacg
      841 aatcctccca cggcaagcgt ggcgtggacg ccgaccagga ttcccgcggg tcgttttttg
      901 aggatgcgtg cggcaagcat caagctgccg actaaagctg ccatcacgat cagtgttgcg
      961 gcaataatta cgaaagagcc cgtgcctcgg tccagtccac gcggcggccc gcgcagcgcg
     1021 agaaagaggc accctaagcc gccaagggcg aaaagcgcgt gcagcaatgc aagcggccaa
     1081 ggcggcggct tggtgccctc ggtatgcaaa tattgaatcg ccaaaccaga gcctatcgac
     1141 accgcaattc cgagggtaat gaaagcagcg attagcatgt cggtcaaaag ttggggtgaa
     1201 gcctaggcgg cagatgattt cagtaaggtg ttacgcgtac aacctagata cattcaccgc
     1261 taaagaagcg agaggcccat tttcttttat catgtcacat ggcgggcatg gtgtcagcat
     1321 ttgctgccat tcgtatgcag cttcagttct ggtcaaaggt cagtggcggc ggcggcattg
     1381 gaagcatgtc ccgcaaccgt ttctcgattt cgtcattgga gaccggcgac gctggcgccc
     1441 ggctgttgtt ggcgatgttc tcgccgaagt gaaccagttt tggcaccgtc agcacaacaa
     1501 cgagtagcac ccattgtgcc acgagaaagg gcgcaagcgc ccgcacaaac ggccggaacg
     1561 gagccggatt ttttaccgcg ccccgcacca tcatcagcgc atagccgaac ggtggcagca
     1621 agaagcttga ttgcaacgtt agtagcacca acaccgatac ccagcgcgcg tcggcaacac
     1681 ggactaacag cggcgggatg acgatcggaa taataacgaa aatgatctcg aaggcatcga
     1741 gcacaaaggc gcagatcccg atcacgccta gcaccacagc tgtcgccggg atgtctccac
     1801 ctggaatacc ggcgaccagg ttgttcacca gccggtcggt gccaagcagc cgcagcacca
     1861 gcgtgaaggt agtggcagcg accaacagcg aaaacaacgc gccggtgatg gtgatggcgt
     1921 cgtgcaggag ttcgcgcaac accttatgat gcagccgccc cgttaagaag ccggcgcaca
     1981 gcaagatgaa ggcgccgacg gcggccgctt cgactgcgta gaaatatccg gtggcgacgc
     2041 cgccgagcag caccagcagg gcgattagcg cagaggtagc gaggatggcc tcttgcggtg
     2101 caaggcgttt gcgttcgccg ggcagcagcg gctggttgcg gcccgcgcgc caagcgagcg
     2161 caagacatag catgataaag atcatggccg ggacgagcgc ggcatggaac acgtcctgcg
     2221 tattaaggac gcggtcggta cggccggttt ccgtaacggc gaaagtgtgc gcattgagt
//
LOCUS       DATCBB010000773         1555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_57614, whole genome shotgun sequence.
ACCESSION   DATCBB010000773 DATCBB010000000
VERSION     DATCBB010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57614"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VII37_08555"
     CDS             <1..1418
                     /locus_tag="VII37_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5069827.1"
                     /translation="AATGAYALYTGYVEPMQQRFEQAGFGGSNLLGLLIFQGAFWKGW
                     QSMLTLLEVSALLTIAASAVVFLRRRIRRSSVFAMMVVGICAAASLSSPAMATEFRSQ
                     GDTVEVEKNETIKGDFFGHGHHVKIEGTVDGDVYVFCQGADIEGHVTGDVIAFAQMLR
                     VTGKVDGNIRSFTNTTLVSGDVTKNLLSFAETVTIDPAGKVGGSITTFVNTLTIDGGV
                     GRDVLAFVNETAIAGKIGGGIQAQGNTLKFNSGAQVDGTVKFKGNKPPEVSSGAKLAT
                     PVVFTQLEHHKPYQESGFYVWRVIWAAASILFGLVLFQLLPNFARATVDSAERYGAAV
                     GLGVLVFFGVPIAALIACITVVGVMIGISTFILWLTALFCAHIVVGAVVGQWLMGHTR
                     ETWQLIGRMVVGVILIRVVEMIPFFGGWVKFAVLLWGMGAISLAIYRHFTPAYTATVP
                     ASPVMPPPLPPNTTIGSPQPA"
BASE COUNT          293 a          449 c          423 g          390 t
ORIGIN      
        1 tggctgccac cggcgcctac gccttgtaca ccggctatgt ggagccgatg cagcaacgtt
       61 tcgaacaggc cggttttggc ggcagcaatc ttctcggtct tttgatcttt caaggcgcct
      121 tctggaaagg atggcaatcg atgcttactc tacttgaggt ctccgctctg ttgacgatcg
      181 ccgcatcggc cgtggtgttt ctgcgccggc gcatccgccg cagctccgta ttcgccatga
      241 tggtcgtcgg aatctgcgcg gcggcttcct tgtcttcgcc ggccatggct actgagtttc
      301 gcagtcaggg cgacactgtc gaagttgaaa agaacgaaac tatcaagggt gatttttttg
      361 gccatggcca tcacgtcaaa atcgaaggca ctgtcgatgg cgatgtttac gtattttgcc
      421 agggtgcgga tattgaaggc cacgtcaccg gtgacgtgat tgccttcgcc caaatgctgc
      481 gcgtcaccgg caaggttgat ggaaatatac gctcctttac gaatacaact ttggtgagcg
      541 gcgacgttac caagaattta ctttcgttcg ctgagaccgt caccatcgac cctgccggca
      601 aagttggcgg cagcattaca accttcgtaa ataccctcac cattgacgga ggtgttggcc
      661 gtgacgttct ggccttcgtg aatgaaaccg ccattgccgg aaaaattggc ggtgggatac
      721 aggcccaagg caacacttta aaatttaact ccggcgccca agttgatggc accgtcaaat
      781 tcaaaggtaa taagccgccc gaggtttctt ccggcgcaaa actggccact cccgttgtat
      841 tcacccagct cgaacatcac aaaccgtacc aggagagcgg cttttacgtt tggcgcgtga
      901 tttgggccgc tgcgtccatt ttatttggct tggtcctttt ccagctcctt ccaaactttg
      961 ctcgggctac ggtggactct gccgagcgct acggagccgc ggtcggcctt ggcgtgctgg
     1021 ttttcttcgg cgtacccatc gccgcactga tcgcgtgtat caccgtggtg ggcgtgatga
     1081 ttggaatttc cacttttatc ctatggttga ccgcgttgtt ctgcgcgcac atcgtagttg
     1141 gcgccgtggt cggccaatgg ttaatgggcc ataccaggga gacctggcag ctgatcggcc
     1201 gcatggtcgt gggcgtcatc ctgattcgcg tcgtggaaat gattcctttc tttggtggct
     1261 gggtcaaatt tgccgtgctg ctttggggca tgggcgccat ttctctcgcg atttaccgcc
     1321 atttcacccc ggcatatacc gcaactgtcc cggcgtcgcc tgttatgccg ccgccactgc
     1381 cgcccaacac caccattggc agcccgcaac ctgcctgacc ttagactgga gcggctcaag
     1441 tcgagcttct gaaaggatcc catccgcgag gctccagaac tcgtgatcgg ccattgccgt
     1501 cagctaccat tacttcacgc gactgggtaa cttccccaat ccgcgtgatc gccac
//
LOCUS       DATCBB010000774         3804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_186355, whole genome shotgun sequence.
ACCESSION   DATCBB010000774 DATCBB010000000
VERSION     DATCBB010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186355"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VII37_08560"
     CDS             <1..339
                     /locus_tag="VII37_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069828.1"
                     /translation="SKVVTQNTEPPLIALIQAGDPALSRLRSGDPKFAKVMDAIDRGQ
                     AQKVSAVDSADFLRAALPLISGGNKFFLGTMEEQRGFDWIVPPDEPVQKTDDKSRPPS
                     APSLRKKPTP"
     gene            complement(313..789)
                     /locus_tag="VII37_08565"
     CDS             complement(313..789)
                     /locus_tag="VII37_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5069829.1"
                     /translation="MAKISHIFRAPKKRLPMEEVEEARVIKNFGLDGCAHARPGGGPR
                     QVLLVDRETLEAMDLRPGVVRENITTEGMDVNSLAMNQELRIGDVRLLVSEICHPCDQ
                     LEKVRPGLRREMRGRRGMLCRVLEGGMIRRGDTIEKVPPVATLPFSEDSRSGLFAK"
     gene            complement(891..2177)
                     /gene="purD"
                     /locus_tag="VII37_08570"
     CDS             complement(891..2177)
                     /gene="purD"
                     /locus_tag="VII37_08570"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY5069830.1"
                     /translation="MKVLLIGGGGREHALEWKLKQSPLVTKIWCAPGNAGIAAEAECV
                     NVDSGDVGAIIALAEKIRPDLTVVGPEVPLVNGIADAFALRNWAIVGPTRQAAKLEGS
                     KIFSKEFLQRHNIPTAQMYGQFDSADKANAALETVKWPVVMKADGLCAGKGVFLAPDK
                     KAAKNFISSVMEEQELGEGGARILLEETLEGEELSFIILTDGQRYAALVPTRDHKRVF
                     DGNEGPNTGGMGAYSSDELLPSALREKIVAQIVEPTLRGLAADGLRYQGFLYIGVMLT
                     ADGPKVLEFNCRLGDPETQAIVARMDFDLAEVLADVAAARVDASKLKWKSGASACVVM
                     ASGGYPGKFEVGKEITGLHEAGEVPGVKVLHAGTKCDGDKIVTNGGRVLGITAAGATL
                     DEALGRVYEAAQQIHFVGAHYRKDIGAHASVRSANR"
     gene            complement(2328..2744)
                     /locus_tag="VII37_08575"
     CDS             complement(2328..2744)
                     /locus_tag="VII37_08575"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5069831.1"
                     /translation="MAWKWPGASSNNVTEEWTGFIDQGVTLEGTLQVSGTFRINGIVK
                     GNIISEHTVILGEGAKVEGQIEGNKVVIAGRFDGVIFAKGRVEIQAKGVVTGEVHAPC
                     MVVDPGGIFDGRCHMLASSDMTSGVTIPIRAAGRAS"
     gene            complement(2854..3150)
                     /locus_tag="VII37_08580"
     CDS             complement(2854..3150)
                     /locus_tag="VII37_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5069832.1"
                     /translation="MIRVVMPAHLRTLASFQGEVELEVQGTVTVRSVLDALEARFPML
                     RGTIREHVTQQRRPFLRFFACGEDLSHDSMDAALPDAVASGAEPLLIIGAIAGG"
     gene            complement(3147..>3804)
                     /locus_tag="VII37_08585"
     CDS             complement(3147..>3804)
                     /locus_tag="VII37_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069833.1"
                     /translation="PGAGGMWLHTILLDPGHPGRIFIAISAAGAFRTDDGGKTWRPIN
                     RGLKSEHIPDPTADVGHCVHRIAMHRSRPNVLFMQKHWDVMRSDNGGDSWQEVSGNLP
                     TDFGFVIDVHAHDPETIYVVPIKSDSEHYPPDGKLRVYRSKTGGNEWEALTRGLPQSD
                     CYVNVLRDAMAVDSMDSCGVYFGTTGGQVYASADAGDNWTPIVRDLPGVLSVEVQTLA
                     "
BASE COUNT          835 a         1369 c          803 g          797 t
ORIGIN      
        1 agcaaagtag tgacgcaaaa caccgaaccg cctcttattg ctttgatcca agccggcgat
       61 ccagctctct cccggctacg ttccggcgat cccaagttcg ccaaagtcat ggacgccatc
      121 gatcgcggcc aggcgcaaaa agtttccgcg gtcgactccg ctgacttcct gcgcgccgcc
      181 ttgcctctga tctctggcgg caacaaattt tttcttggca ccatggaaga acagcgcggc
      241 ttcgactgga tcgttccgcc agatgaacca gtgcaaaaaa cggacgacaa atcgcgcccg
      301 cccagcgcgc cttcacttcg caaaaagccc actccgtgag tcttccgaaa atggcaaagt
      361 cgccaccggt ggaaccttct caatcgtatc gcctctccga atcatcccgc cttccagcac
      421 ccggcacaac attcctcgcc gcccacgcat ctcccgccgc aatcccggcc gtactttttc
      481 cagctgatcg cacggatggc aaatctcgct caccagcaag cgaacgtcgc cgatgcgcag
      541 ctcctgattc atcgccagac tattcacatc cattccttcg gtcgtaatat tttcgcgtac
      601 aactcccggc cgcagatcca tcgcctccaa cgtttctcga tccaccagca aaacttgtct
      661 cgggccgccg cccggccgcg catgcgcgca accgtccaac ccaaaattct tgatgacccg
      721 cgcttcttca acctcttcca ttggaagccg cttcttcggc gcgcgaaaaa tgtgcgagat
      781 ctttgccatg actcttctcc taattacccg caaaaactta ccgaacacgg agggtgcccc
      841 accctcagat atttggatgg tggggtttgt aacgtgggat catcacccaa ctaacggtta
      901 gcactcctca cgctcgcatg tgccccaata tcttttcgat agtgcgcgcc cacaaaatga
      961 atctgttgcg ccgcttcata cacgcgcccc agcgcctcat ccaaagtcgc tcccgccgcg
     1021 gtaatcccta acacccgccc gccattcgtc acaattttgt caccgtcaca tttcgttccg
     1081 gcatgcaaca ccttcacgcc aggcacctcg ccagcctcat gcaatccggt aatctccttg
     1141 cccacctcaa atttccccgg atatcccccc gatgccatca ccacgcacgc actcgcgccg
     1201 cttttccatt ttaatttcga cgcatccacc cgtgccgcgg ccacatccgc caacacctcc
     1261 gccaaatcaa aatccatccg cgccacgatc gcttgcgtct ccggatcgcc cagccggcaa
     1321 ttgaactcca gcaccttcgg cccgtccgcg gtcaacatca ccccaatata caaaaagccc
     1381 tggtagcgaa gtccatcggc cgccaatccc cgcagcgtcg gctccacaat ttgcgccacg
     1441 attttttcac gcagcgcgct cggcaacaat tcgtcgctcg aataggcccc catcccacca
     1501 gtattcggcc cctcattccc atcaaaaact cgcttgtggt ctcgcgtggg caccaacgcc
     1561 gcatagcgct ggccatccgt caaaataata aacgacagtt cctcgccttc cagcgtctct
     1621 tccaacaaaa tccgcgctcc gccctcaccc agttcctgtt cctccatcac gctggaaata
     1681 aaattcttcg ccgccttttt atccggcgcc aaaaaaaccc ctttgcccgc gcataatcca
     1741 tccgctttca tcaccactgg ccatttcacg gtttcgagcg ctgcgttggc tttgtccgcg
     1801 gaatcaaact gcccatacat ctgcgctgtg ggaatattgt gccgctgcaa aaactccttc
     1861 gaaaaaatct tgctgccttc caacttcgcc gcctgtcgcg taggtcccac aatcgcccaa
     1921 ttccgcagcg caaacgcatc cgcaattcca ttcactaggg gcacctctgg tccgaccacc
     1981 gtcaaatccg ggcgaatttt ctccgccagc gcgatgatcg cccccacatc acccgagtcc
     2041 acattcacgc attccgcttc cgccgcaata cccgcattcc ctggagcgca ccaaatcttc
     2101 gtcactagcg ggctctgctt cagcttccac tccaacgcat gctcacgccc gccaccacca
     2161 atcagcaaca ccttcatgcg ctagccctca tcttcaggat ccactcacac caccgtcatc
     2221 gacattatag acggaaattt aaatcgtaaa ttcctgaagg aattcagtag ttggaattgt
     2281 tacagaagga attacgcaga agtattattg ccaaccgtca aattctgcta agaagcccgc
     2341 ccagccgcac gaatcggaat cgtaactccc gaagtcatgt ccgatgacgc caacatatgg
     2401 catcgcccat caaaaatccc gcccggatcg accaccatgc acggcgcatg cacttcgccg
     2461 gtaactacgc ccttcgcctg aatctccacg cgccctttcg cgaaaatcac gccgtcgaat
     2521 cgccccgcga tgaccacctt attgccttca atctgccctt caactttcgc gccttcgccc
     2581 aatataacgg tgtgctcgga aataatattg cccttcacaa ttccgttaat gcgaaaagtt
     2641 ccgctgacct gcagcgtccc ttccagcgtc actccttgat caataaatcc ggtccactct
     2701 tctgtgacgt tattactcga tgcgccgggc catttccagg ccattaaagc cccctcggta
     2761 gagactcttc tactctagcc gcgcgcaccg tcaagtcaac gcaacccatt cccaaagtgt
     2821 cgaatgcacc gttgaccgaa tggccagtcg acttcatcca cccgcgatgg ccccaatgat
     2881 aagcagtggc tcggcacccg aagctactgc atccggtagc gcggcgtcca tcgaatcgtg
     2941 cgacaaatct tcgccacacg cgaaaaatcg caaaaacggc cgccgctgct gcgttacatg
     3001 ctcgcgaatc gtcccgcgca gcatcggaaa tcgcgcttct aacgcatcca gcaccgatcg
     3061 caccgtcacc gtgccttgta cctcaagctc cacttcaccc tgaaaagacg caagcgtccg
     3121 caagtgcgcc ggcatcacca cgcgaatcat gccagcgtct gcacttccac cgaaagcacc
     3181 cccggcagat cccgcacaat cggcgtccag ttgtcccccg catcggccga cgcataaacc
     3241 tgtccgccag tcgtgccaaa atatacgccg cacgaatcca tggagtccac cgccatcgcg
     3301 tcgcgcaaca cattcacgta gcaatcgctc tgcggcagcc cccgcgtcag cgcttcccac
     3361 tcatttccgc cggtcttact gcggtacaca cgcagtttcc cgtccggcgg ataatgctcc
     3421 gagtcgctct tgatgggcac cacgtagatc gtctccggat cgtgcgcatg cacgtcgatc
     3481 acaaatccaa aatccgtcgg caagttaccg ctcacttcct gccacgaatc tccgccattg
     3541 tcgctgcgca tcacgtccca gtgtttctgc atgaagagca cattcggccg cgaacggtgc
     3601 attgcgatgc ggtgaacgca gtggccgacg tcggctgtcg ggtccggaat gtgttcagac
     3661 ttcagtccgc ggttgatcgg ccgccatgtc ttgccgccgt catccgtgcg gaacgcgccc
     3721 gcagccgaga tggcgatgaa tattcggcca ggatggcctg ggtccagcag gattgtgtgc
     3781 agccacattc caccagcacc tggc
//
LOCUS       DATCBB010000775         1185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_414597, whole genome shotgun sequence.
ACCESSION   DATCBB010000775 DATCBB010000000
VERSION     DATCBB010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414597"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /gene="alr"
                     /locus_tag="VII37_08590"
     CDS             complement(<1..750)
                     /gene="alr"
                     /locus_tag="VII37_08590"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5069834.1"
                     /translation="MSRASKNSTTLNMRPVWADVSLPALVHNFKAIRAYVNPSEANKT
                     KEKRGAPRKVLCIVKGNAYGHGGPAVAAALEKAGSDWFGVTCTEEGITLRKAGVRKPI
                     LILTSFVAGEEKRLLDHNLTAVIHRCEQLAQLERAAAKRGTKKVSFHLKIDSGMNRLG
                     IAPGDVDCFARQLAKARHLQLGGVMTHFASSGVFTDTPVGRQTREQEEKFYAALARLR
                     ALGIDTCIVHLANSAAIVARPESWADMVRPGA"
BASE COUNT          233 a          385 c          317 g          250 t
ORIGIN      
        1 tgcgccgggc cgcaccatgt ccgcccagga ttccggccgc gccacaatcg ccgcgctgtt
       61 ggccaggtgc acgatgcagg tatcgatgcc cagcgcgcgc aaccgcgcaa gcgcggcata
      121 aaatttttct tcctgctcgc gggtctgccg ccccaccggc gtgtcggtga aaactcccga
      181 cgacgcgaaa tgggtcatca ctccgccgag ttgcagatgc cgcgccttgg ccagttgccg
      241 cgcgaagcaa tccacatcgc cgggcgcgat gcccaggcga ttcatgcccg aatcaatttt
      301 cagatgaaac gaaacctttt tcgtgcctcg cttggccgct gcgcgctcca gctgcgccag
      361 ctgttcgcag cggtgaatca ccgccgtcaa attgtgatcc agcaggcgct tttcttcgcc
      421 ggccacaaaa ctggtcagga tcaaaatggg tttgcgcacc ccggctttgc gcagggtgat
      481 gccctcttcc gtgcaggtca cgccaaacca gtcggacccg gccttttcca gcgccgcggc
      541 taccgcaggc ccgccgtgcc cgtaagcatt gcctttcacg atgcacagca cttttcgcgg
      601 cgcaccgcgt ttttcttttg ttttatttgc ctccgacgga tttacatacg cgcgaatcgc
      661 tttgaaattg tgcaccagcg cgggaagcga aacatccgcc caaaccggcc gcatattcaa
      721 cgtcgtggaa tttttcgaag ctcgcgacat gttcttccag ggaaaataag tttacgccat
      781 ccaagcggca gcggctccca tgcgcctgaa aactgccgcg tgaatgacaa cagcgggcaa
      841 ctgctgccca tttataagca cgccgtcagc cacaagcgga aactctctcc cgagaaatcg
      901 ccgtaaatga actcccggct aacatcagct atcgattgag tttgcggatg aaggttgggg
      961 cctgccgcgc agcaggctgg ccggcattgt acggaggcct cgtggccgtg ctgccacgct
     1021 gtggctgccg ttacatatct tcggcagcaa actgcgaaaa cgcatccggc gccgcttctt
     1081 caaacctggt caaccggctg cgaaacacaa atttcacgct ctcggtaggc ccgttgcgct
     1141 gtttggcaat cttcaactcg gtctcttcgc gcatttcctg cgtaa
//
LOCUS       DATCBB010000776         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_381197, whole genome shotgun sequence.
ACCESSION   DATCBB010000776 DATCBB010000000
VERSION     DATCBB010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381197"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..810)
                     /locus_tag="VII37_08595"
     CDS             complement(196..810)
                     /locus_tag="VII37_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069835.1"
                     /translation="MKSANSAALGFRAHSGWAATVALGGSLRSPEVLDRRRIALIESG
                     SPGGVQPYHVARTMDLPKAEEFIKGVIAAIDRAALIAVRDLAEALNAMGQQVSCCGIV
                     LASGRALPSLEATLRSHAMVHTAEGELYRAAIARAAKNLSWRCVRVPERELYKLAANR
                     LRTPELKLKVRITEMGRAVGSPWSADEKCASLVAWLALAGFEAS"
     gene            complement(828..1856)
                     /gene="era"
                     /locus_tag="VII37_08600"
     CDS             complement(828..1856)
                     /gene="era"
                     /locus_tag="VII37_08600"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY5069836.1"
                     /translation="MPDKKTLDKKAPEKNSEVAPLQAGFKSGFVAIVGRPNAGKSTLV
                     NRLVGQKIAIVTSKPQTTRNRIQGIVTKPEGQIIFIDTPGLHDADSALGRQMMHEVAA
                     ALEGIDVLLLMVDASRAYPHADALLLQKAERFRGKTILALNKVDRLPKPKLLPLIDAF
                     QKAFDFAAIVPISALTGAGCDDLMEAIMRELPPGVPYFPDDQITDQPERFLAAEIIRE
                     KAIQVMYHEVPYALAVIVDKFEELPKLLRIEVTMNVERDSQKKILIGHKGEMLKKIGT
                     EARKELEKLFDITIYLALFVKVVPDWRENPQKVRELDWHFQLEGLSEEIARKSPRDEG
                     PSKVPPGE"
     gene            complement(1908..>2736)
                     /locus_tag="VII37_08605"
     CDS             complement(1908..>2736)
                     /locus_tag="VII37_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY5069837.1"
                     /translation="KENEKSAEQRTEEGIEALVEAAEEEGIIEPEQADLIEQVVEFSD
                     KRVREVMTPRPDIIAIAAEATLEELHAKFVEHKFSKMPVYEKTLDDIFGVVNAQDLLH
                     IADQDLPRRKVRELAKPVLFIPETKAGSDLLREMRQKGQPLAVVIDEHGSVAGIATVE
                     DLVEEIVGESGYDAKQPLADVVRETDGGLVMRGSMSIDDVEELLGVKFGDKSDAAVTT
                     IAGLLSHVSRKVPEPGDKIDLEGHKFEVLEANQRKVLRVRIRKQVAAPVSQLQAPKR"
BASE COUNT          563 a          793 c          720 g          660 t
ORIGIN      
        1 tccgatgtgt gctgagtgga aggactgacg ctgttggtgc ttcgagattg tgaatcggct
       61 gcggaggatt gcgtcccgag cgattggaga ggaagaccta aagtaaaagt tgcgagataa
      121 gcgcacgcgg aaattgaagt ccggcgaagt ccgtcaagca tcgttccccc ggcagactgc
      181 gatgtctgct caatattaag atgcttcgaa accggctaac gccagccagg cgaccaacga
      241 agcgcatttt tcgtcagcgc tccacggact acccacggct cggcccattt cagttatgcg
      301 aaccttcaac tttagttcgg gagtgcgcag gcgattcgcg gccaacttgt aaagctctcg
      361 ctcgggaacc cgcacgcagc gccaacttag atttttggcg gctctggcga tggcggcccg
      421 gtacaattcg ccttcagcgg tgtgcaccat cgcgtgcgac cgcagcgtgg cttccagcga
      481 aggcaaagcg cgacccgaag caagaacaat tccgcaacag cttacttgct gccccatcgc
      541 gttgagcgct tccgcaagat ctcgtacggc gatcagcgcc gcgcggtcaa tcgctgcgat
      601 cacgccttta ataaattctt cggctttggg cagatccatc gtgcgggcga catggtaggg
      661 ctgaacgccc cccggcgagc ctgactcgat cagcgcaatg cgccggcgat cgagcacttc
      721 tggcgaccgc agcgatccgc cgagagccac cgtagccgcc cagccggaat gcgctctaaa
      781 tccgagcgcc gcgctgttcg cacttttcac aagacgtcct ggtgttgcta ttcgccaggc
      841 gggactttgc tcgggccttc atccctgggc gacttccgcg caatttcttc gctcagaccc
      901 tccagctgaa aatgccaatc cagttcgcga accttctgcg gattctcgcg ccagtccggg
      961 accactttca caaacaaggc cagataaatt gtgatatcaa aaagtttttc cagttccttg
     1021 cgggcttcgg tgccgatctt tttaagcatt tcgcccttgt ggccgatcaa aatctttttc
     1081 tgagaatcgc gctccacgtt catggtcact tcaatacgca aaagtttcgg cagctcctcg
     1141 aatttatcta cgataacggc gagcgcgtaa gggacctcgt gatacatgac ctgaatggct
     1201 ttttcacgaa taatttccgc ggctaaaaaa cgttccggct gatcagtgat ttgatcatct
     1261 ggaaagtagg ggacgccggg gggcagttcg cgcataatcg cttccatcaa atcgtcgcaa
     1321 cccgcgccgg tcagcgcgga aatggggacg atcgcggcga aatcaaaggc cttttgaaaa
     1381 gcatcgatca gcggaagaag cttgggttta ggaagccggt ctactttatt cagcgccagg
     1441 atggtctttc cccggaagcg ttcagccttt tgcaggagca acgcgtcagc gtggggataa
     1501 gcgcggctcg catctaccat gagtaacagc acgtcgatgc cttcgagggc agcagcaact
     1561 tcgtgcatca tctggcggcc cagcgcggaa tccgcatcgt gcaaccctgg cgtatcgata
     1621 aaaataatct ggccttccgg cttggtgaca attccttgga tgcggtttcg ggtggtctgc
     1681 ggcttggaag tgacgatggc gattttttgc ccgaccaggc gattcacgag cgtcgatttc
     1741 ccagcattcg gccggcccac gatggccacg aatccggact taaatccagc ctggaggggc
     1801 gcgacttcac tatttttttc tggagctttt ttgtctagcg tttttttgtc gggcattgtg
     1861 attttctgaa tgttgaatct gctactcaaa tgaccggcgt gcggaattca gcgttttgga
     1921 gcttgcaact gactgacggg tgccgccact tgtttgcgta tgcgcacgcg caaaactttt
     1981 cgttgattgg cttccagcac ttcgaacttg tggccttcca gatcgatctt gtcgcccggc
     2041 tccggaactt tacgcgaaac gtggcttagc aggccggcaa tagtggtgac cgccgcgtcc
     2101 gacttatcgc caaactttac gcccagcaat tcctcaacat cgtcgatgga catgctgccg
     2161 cgcatgacca ggccaccatc cgtttcgcgc accacgtcag ctaacggctg cttggcatcg
     2221 taaccgctct caccgacgat ttcttccacc aaatcttcca cggtggcaat acccgcgaca
     2281 gatccgtgct catcgattac caccgctagc ggctggccct tttgccgcat ctcacgtaga
     2341 agatccgatc ccgccttcgt ttcgggtatg aataacaccg gcttggccag ttcacgcact
     2401 tttcgccttg gcaaatcctg gtccgcgatg tgcaacaaat cttgtgcgtt gaccacgccg
     2461 aaaatatcgt ccagcgtttt ttcataaaca ggcatcttgg aaaatttatg ttctacgaac
     2521 ttggcgtgca attcttccag cgtagcttcg gcggcaatgg caatgatgtc cggtcgtgga
     2581 gtcattacct cacgaaccct cttgtcgctg aattccacca cctgctcgat cagatcggct
     2641 tgctcaggct cgatgatgcc ctcttcttcg gcagcttcga ccagcgcctc gatgccttct
     2701 tcggtgcgct gctctgcgga tttctcattt tccttt
//
LOCUS       DATCBB010000777         2477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_220103, whole genome shotgun sequence.
ACCESSION   DATCBB010000777 DATCBB010000000
VERSION     DATCBB010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220103"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VII37_08610"
     CDS             complement(<1..428)
                     /locus_tag="VII37_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HEY5069838.1"
                     /translation="MKLSEIAGKLGCRIEGPQDTEITGVAGIDHARPGQVAFLANRRY
                     FPLLKTTQASAVLLQAGIALERAPGTPAISALRSENPYLDFARTIEFFYQPPAYAPGI
                     HPTAVIAKSAEISEDAHVGPYCFVDEEAQIGRNAVLHSFVT"
     gene            572..952
                     /locus_tag="VII37_08615"
     CDS             572..952
                     /locus_tag="VII37_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069839.1"
                     /translation="MTNQLGLGLRFRLGAIAILSIALPALAQQDQMAPPRLSLSSPLE
                     IPLPKLAIPAQSSQPAQASQGDKDKPKKPKTNDEKAETATPATSNDRLFFALPNFLSV
                     ESADKIPPLTTKQKFAVVTRSSFD"
     gene            945..1433
                     /locus_tag="VII37_08620"
     CDS             945..1433
                     /locus_tag="VII37_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069840.1"
                     /translation="MTNTVPWYAFLAGVSQAENSEPGYGQGAAGYGKRFGSAAADGTI
                     ENYWTSAILPSVFHQDPRFYQLGKGGFWHRTFYAMSRIVVTRSDSGSSQFNVSEVFGS
                     AIAASISTYSYHPHADKTLSNTASVWGSQIAYDTATIVLKEFWPDIRRKLTHKNSTPV
                     AP"
     gene            complement(1537..>2477)
                     /gene="opgC"
                     /locus_tag="VII37_08625"
     CDS             complement(1537..>2477)
                     /gene="opgC"
                     /locus_tag="VII37_08625"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEY5069841.1"
                     /translation="SLRLYGYHALLLAFALLVAVPIASRGNRPGLHNLLDFYFYAPRR
                     AVGEAFLLIYRPPLLDILPMYIIFLALTTIALVLSRRFGWKPILWGGFALYFIAQFGF
                     RNAEHNFMMRFIPTRIPLNEMGSFDLWAWQFLWIVGIWFGVRWAQDRLPLEIWARRAI
                     IPAAVLVCAFFILRREIAHGFELGAAEFLFDKWHLGIMRLVNFAAAATVLIYFQSVLK
                     PLAIKPLVLMGQSSLQVFCVHLLFCFAGLTLMGNASMLSGWKQLGLLTGTFTAMLLTA
                     ILFSKSEAKQERQPKAGLPLEPGQPAAVAAARSAVA"
BASE COUNT          610 a          698 c          674 g          495 t
ORIGIN      
        1 gtgacaaagc tatgtaacac cgcattgcgc ccgatttgcg cttcctcatc cacgaagcag
       61 taggggccaa cgtgggcgtc ttcactaatt tccgcgcttt tggcaatcac tgcagtgggg
      121 tgaattcccg gggcatatgc cggcggctgg tagaaaaatt caatggttcg cgcgaaatcc
      181 agatacggat tttcgctgcg tagcgcggaa atagccggcg taccgggcgc tctttccagc
      241 gctataccgg cctggagtag caccgccgac gcctgcgtcg ttttcaaaag agggaagtag
      301 cgccgattgg cgaggaaagc aacctgcccc ggcctggcat gatcaatccc agcgacgccg
      361 gtaatttccg tgtcttgcgg gccttcaatg cggcaaccca gcttgccggc aatttccgat
      421 agcttcatga acatcgctcc tgacagcagt gcgagtctcg cacagcgccg ggaacgaagt
      481 ataggcactc acgagtgccg cgggtattaa acacgtacag caagggcatg tggattttgg
      541 gcaagactga ccttgtgaga cgattaatag catgactaat caactgggtt taggcttgcg
      601 attccgttta ggggccatcg ccatcctatc gatcgcgctg cccgcgcttg cgcaacaaga
      661 tcaaatggcc ccgccgagac tgtcgctttc ttcgccgctt gaaattcctt tgcccaagtt
      721 ggctatcccc gctcagtctt cgcagcctgc acaggcttcc cagggcgaca aggacaagcc
      781 caagaagccg aagaccaatg acgagaaagc cgagacggcc acacccgcca cctccaacga
      841 ccggctcttc ttcgcgctgc ccaacttcct gtcagtggag agcgccgaca agattccgcc
      901 cctcactacc aagcaaaagt ttgccgtggt aactcgttcc tcctttgact aatacagtgc
      961 cctggtatgc attcctagcc ggagtcagcc aggcggaaaa cagtgagccg ggttacggac
     1021 aaggcgcggc tggctacggc aagcgatttg gatcagctgc cgctgatggc accattgaga
     1081 actactggac ctccgcgatt cttccttcag tttttcacca ggatccgcgt ttttatcaat
     1141 tggggaaggg cggattttgg catcgcacgt tttacgccat gagccggata gtagtgaccc
     1201 gctcggattc cggcagcagt cagttcaacg tttctgaagt gtttggcagc gccatcgccg
     1261 cgagtatctc aacctacagt tatcatcccc atgccgacaa gaccttgtct aacactgcca
     1321 gcgtatgggg cagccagatt gcgtacgaca ccgcgacgat cgttctgaaa gaattttggc
     1381 ccgacattcg ccgaaaactt actcacaaga acagcacgcc ggtggcgcct taatagcccc
     1441 aagcacgtca gcgaccgctg ttatgccgca gccgaccaaa aagaaaatcc tggattcagt
     1501 tccaagttta aaagactggg gagcggaaga ctatagctaa gcaacagcgg atcgcgcggc
     1561 ggctactgct gcgggctgcc cgggctcaag tggtaggcct gctttcggtt ggcgctcttg
     1621 tttggcctca ctcttcgaga atagtatcgc cgtgagcagc atggccgtaa aggtgccggt
     1681 cagcagtcca agttgcttcc aaccgctcag catggaagcg ttgcccatca gcgtgagtcc
     1741 cgcgaaacaa aatagcaggt gtacgcaaaa tacttgcagc gaagattggc ccattagtac
     1801 gagcggcttg atggccagcg gctttaatac cgattgaaag tagatgagca cggtagccgc
     1861 cgccgcgaaa ttcaccaggc gcatgatccc cagatgccat ttgtcaaaaa gaaattccgc
     1921 ggcgcctaac tcaaagccgt gcgcgatctc gcggcgcaga ataaagaacg cgcacaccag
     1981 cacggcggcc ggaattatcg cgcgcctggc ccaaatctcc agggggagcc tgtcctgtgc
     2041 ccatcggacg ccaaaccaga tgcccacgat ccacaaaaat tgccaagccc acagatcaaa
     2101 ggaccccatt tcatttagag ggattcgagt gggaatgaag cgcatcatga aattgtgttc
     2161 cgcattacga aaaccgaatt gtgcgatgaa ataaagtgcg aaacctcccc acagaatggg
     2221 cttccatccg aacctgcgtg acagcacaag cgcgatagtc gttaaggcca aaaaaataat
     2281 gtacatgggc aaaatgtcga gcagtggtgg gcggtaaatc agcagaaatg cttcgccaac
     2341 ggcgcgtctt ggagcgtaga aataaaaatc cagcaggttg tgtaatccgg gacggttacc
     2401 gcgagaggcg atcggaacag cgacgagaag cgcaaaagcg agtaacagcg cgtggtagcc
     2461 gtacagacgc aggctgc
//
LOCUS       DATCBB010000778         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_381210, whole genome shotgun sequence.
ACCESSION   DATCBB010000778 DATCBB010000000
VERSION     DATCBB010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381210"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VII37_08630"
     CDS             <1..601
                     /locus_tag="VII37_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5069842.1"
                     /translation="MEKQLPKGSHIIIRGQVQTMKTSFTGLGFGLLGAIGLAYLLIVV
                     NFQSWLDPFIIITALPGALAGICWMLLITHTTLSVPSLTGAIMCMGVATANSILMVSF
                     AREQMDEGKNPMDAAVEAGFVRMRPVIMTALAMIIGMMPMAIGLGEGGEQNAPLGRAV
                     IGGLLFATFATLFFVPCVFSVIHGWLDKRKAHGKEAATA"
     gene            635..2011
                     /locus_tag="VII37_08635"
     CDS             635..2011
                     /locus_tag="VII37_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5069843.1"
                     /translation="MSQDVMEQKDQGTAGERTPVREQPRTQQAQRQPERSSLFVVLVI
                     CAVLLLVVGGITLLMRRSQYKALAKETETLAVPTVSVVHPIAESDSEDLVLPSTLQAY
                     VESPIYARTNGYLKKWYHDIGSRVNKGELLADIDTPEIAQELAQSRAELGTAQANQNL
                     SQVTASRYEGLLKTDSVSKQEVDNATGDLAAKRANTLSAEANVRRLQETESFRHIYAP
                     FSGVVTRRNVDIGNLINAGNGGTAQLLFTLAQTDPIRVFVNVPEVYAPAIHQGLGAYL
                     ELQQFPGQKFYGKVARTSDSIDLNTRTLLTEVDVPNKSGQLLPGGYAQVHLEVKVNGS
                     RLEVPVNALLFRSEGLRAVIIDDKHKTHLQPLMIGRDYGTTLEVLQGLKPTDWIVINP
                     ADSLDEGVQVNVKQIAQPAAPAPASTGTGASSGQGNPTNNGTRPPSGTPAANPNGSNN
                     GQSAPKNQ"
BASE COUNT          512 a          570 c          633 g          483 t
ORIGIN      
        1 aatggaaaag caattgccca agggaagtca catcataatt cgtggccaag ttcagaccat
       61 gaaaacttcc ttcacgggct tgggatttgg actcctggga gctattgggc tggcttattt
      121 attgatcgtt gtaaattttc aatcctggct ggatcctttc atcatcatca ccgcattacc
      181 tggcgcgttg gcaggaattt gctggatgct gttgatcacc cataccacgc tgagcgtgcc
      241 atcgttgacc ggcgcgatca tgtgcatggg cgtggctacc gccaatagca ttttgatggt
      301 ttcgttcgcg cgtgagcaga tggacgaagg caaaaatccc atggacgccg ccgtggaggc
      361 tggctttgtg cgcatgcgtc cggtgatcat gaccgcgctg gcgatgatca tcggcatgat
      421 gcccatggcc atcgggcttg gcgaaggcgg cgaacaaaac gcaccgcttg gtcgcgcggt
      481 gattggcgga ttgctgtttg ccacgttcgc aacattattt tttgtgcctt gcgttttttc
      541 cgtgattcac ggctggctgg acaaaagaaa agcgcacggc aaagaggcgg caacggcata
      601 aaatgaatga ttgcgtaaat ctgcggagcg aagtatgagt caggatgtta tggagcaaaa
      661 agatcagggc acagcagggg agcggacgcc ggtaagggag caaccgcgca cgcagcaagc
      721 ccaacgacaa cccgagcgca gcagtctgtt cgtagtgctg gtgatctgcg cggttttatt
      781 gctggttgtg ggggggatca ccctattgat gcgccgctcg cagtacaaag ccctggcgaa
      841 ggagacggaa actctcgcgg tacctacagt atctgtagtt cacccgattg cggagtcaga
      901 ctctgaagat ctggtactgc cttccacgct acaagcctat gtggaatcgc caatctatgc
      961 gcgcaccaat ggctacctga agaagtggta tcacgacatt ggcagccgcg tgaataaagg
     1021 cgaacttctc gcggacatcg atacgccgga aatcgcgcag gaattggcgc aatcacgcgc
     1081 ggagttggga actgcgcaag ccaatcagaa tctttcgcag gttaccgctt cacggtatga
     1141 gggcctgctt aaaacggata gtgtgtcgaa acaagaagtg gacaatgcca ccggcgatct
     1201 cgctgcgaaa agggccaaca ctctttcggc cgaagccaat gtccgcagat tgcaggagac
     1261 ggaatcgttc cggcatattt atgctccgtt cagcggcgta gtcacacgcc gcaatgtgga
     1321 tatcgggaac ctgatcaacg cgggaaatgg cggtacggcg cagttattgt tcacgttggc
     1381 gcagacagat cccatccgcg tgtttgtgaa tgtgccggaa gtttatgccc cggcgattca
     1441 ccagggactc ggcgcgtacc tggaattgca acaattcccc ggccaaaaat tttacggcaa
     1501 agtcgcgcgc acctccgatt ccatcgacct gaatacccgc acgctgctga cggaagtgga
     1561 cgtgccgaac aagtcggggc aattgttgcc ggggggctat gcgcaagtgc atctggaagt
     1621 gaaagtgaac ggctcccggc tggaagttcc ggtgaatgct ttgctgttcc gctccgaagg
     1681 tttgcgcgcg gtcatcattg atgacaaaca caaaacgcac ttgcagcctt tgatgattgg
     1741 ccgcgactac ggcaccacgt tggaagtatt gcagggctta aaacccaccg attggatcgt
     1801 aatcaatcct gctgattcac tcgacgaagg tgtgcaggtc aacgtaaagc agatcgcgca
     1861 acctgccgcg cctgcgccgg cgagtacggg gactggcgct tccagcggac aggggaatcc
     1921 cacaaataat gggacgcgac cgcccagtgg gacgcccgct gcaaatccca atggcagtaa
     1981 caacggtcag tccgcgccta aaaatcaata atttggcctg atggccttta cttcaattat
     2041 gagtatcctg aacttcaaac aaaattattt tgcctttcgc ttcagtgtgg cggttggcag
     2101 cttctgtttg gccgcgtgct tcgccgggtg cactgtggga ccaaattatc atcgcccaac
     2161 cgcaccggtg cctgccactt gggacgtgca ggaacctt
//
LOCUS       DATCBB010000779         1135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_349269, whole genome shotgun sequence.
ACCESSION   DATCBB010000779 DATCBB010000000
VERSION     DATCBB010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349269"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..575
                     /locus_tag="VII37_08640"
     CDS             240..575
                     /locus_tag="VII37_08640"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069844.1"
                     /translation="MLHDLAEAAGGKGIHLMAPLQVLVTHDEAHAIARGLVSDLSVQF
                     PDEYILSAQARRRGRMFLAYLRNGRGTPRPRAREGFPIAAPVTWSRIESGIQPDAFTV
                     DRPFRAPPR"
     gene            903..>1135
                     /locus_tag="VII37_08645"
     CDS             903..>1135
                     /locus_tag="VII37_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069845.1"
                     /translation="MSGLVLAPDKPGARAAVDRAPLLRWLIFTGVTVFAATLLWRYGL
                     IRLMLASDRTYISSVICLLYVAASLHCLWRTIAL"
BASE COUNT          219 a          323 c          333 g          260 t
ORIGIN      
        1 cagccttatt ctggtggggt ctatcgcact ggtgctcaac ctcacagact agcgtggaaa
       61 ccgcgcgcgg ctggcgcctt tccgaaattc tgcgcgcgtg ggtcgcatca gctcaagggc
      121 taccggtccg ctgggatttg agcgcgcaat gcatggctgc attggcagat attgttgaaa
      181 aagtcctttt tgggtgacga acgaaatttc ttagggccgc tagtggacaa acctcggcca
      241 tgcttcacga cttggccgaa gctgcgggcg gcaagggcat tcatctgatg gcaccgctac
      301 aagtgctggt cacccacgat gaagcccatg cgattgcgcg gggtcttgtc tccgatctca
      361 gcgttcaatt tccagacgaa tacattttgt cagcgcaagc gagacggcgc ggtcgcatgt
      421 tccttgccta tttgcgaaac gggcgcggca caccgcgacc ccgggcgcgc gaaggttttc
      481 caattgccgc accagtgact tggtcccgca ttgaatcggg catccagccc gatgcgttca
      541 cggtggaccg cccattccgt gcgcctccga gatagacata gcgagtcgct tgcgggtgtc
      601 ggagccccgc aatatcaaac cggaatgcac atcagagtga acgttcgtta gcaattctgg
      661 atctgaaggg tttaaggatt gtggcaaact tccagctccg ggaacaaacc tggctatata
      721 ctgatcgttt ccgatacgct cgaagtggaa aacttgacag cgaagatcgg attgaacctg
      781 acctgatctg cgccacagat tcgcatctgg acgtttgacg gaccttgtgg aatgttatcg
      841 ctggaaggcg tcgcgctgca tgctagaaaa gtcttggccg ctcggggagg gagccttaag
      901 acatgagtgg gctggtcctc gccccagata agccgggcgc gcgcgccgcc gtcgatcgcg
      961 cgccgctatt gcgctggctt atcttcaccg gcgtgacggt tttcgcggcg accctgctat
     1021 ggcgctacgg cctcattcgc ctgatgttgg cttctgaccg cacgtacatc tcctcagtca
     1081 tttgcctgct ctatgtcgcg gcgtcgctgc attgcctttg gcgcacgata gcgct
//
LOCUS       DATCBB010000780         1168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_381241, whole genome shotgun sequence.
ACCESSION   DATCBB010000780 DATCBB010000000
VERSION     DATCBB010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381241"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VII37_08650"
     CDS             complement(<1..313)
                     /locus_tag="VII37_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5069846.1"
                     /translation="MNLIAAQKAAMEAARAAGKIMRTNWHEPKRVNSTEAHDIKLELD
                     VRCQALIEKILARAFPQIPVLGEEGSTGDVNAEYRWVIDPIDGTVNYFFGMPHACVSI
                     AL"
     gene            complement(377..>1168)
                     /locus_tag="VII37_08655"
     CDS             complement(377..>1168)
                     /locus_tag="VII37_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069847.1"
                     /translation="KPVYPMRLTGMDSPSLSVDLYVFGSASATAPLFKVESCTRPNVV
                     HPLLREWVGDSPVATKLTATLSPAEMRQDVWLDQSPFFLEHKNRLFSRHGALTIALNW
                     GAGLFAAGLFAACLLAFASETYKIKLSRQISVITLACVMLAGLIYFSLPKIEVKLVKG
                     GYFHFYNELKEEQLALSMALDDFDWHTATEARAGLREITSNSTNAANYGLKNWDNDFV
                     GGRLREEDSPGNYLLRETNNQLELIFINRDGGEETSTTKNLPGWH"
BASE COUNT          273 a          305 c          323 g          267 t
ORIGIN      
        1 gcagcgcaat cgagacgcag gcgtgcggca tcccgaagaa ataattcacc gtgccgtcga
       61 ttgggtcaat gacccagcga tattcggcat tcacatcgcc cgtgctgcct tcctcgccaa
      121 ggacgggaat ctgcggaaag gcgcgggcaa gaattttttc gatgagcgcc tggcaacgca
      181 catcaagttc gagcttgatg tcgtgggcct cggtagaatt cacacgcttg ggttcgtgcc
      241 agttggtgcg catgattttt ccggcggcac gcgcggcctc catggcggct ttttgggcgg
      301 caattaaatt cacagccaga cttgatgcca gcagaacgac cggcggtcga gattttttgg
      361 aaccgccgac aaggatttaa tgccagcccg gcaaattttt tgtggtagaa gtctcctcac
      421 caccatcacg gttaataaaa attagttcca actggttgtt ggtttcacgt agcaaataat
      481 ttcccggtga atcttcttca cgcaaccgcc caccaacgaa atcattgtcc cagtttttca
      541 ggccatagtt cgcggcattg gtcgaatttg aggtgatttc ccggaggccg gcgcgcgctt
      601 cggtggcggt atgccagtca aagtcatcca aggccatgct aagagccagt tgctcttctt
      661 tcaattcatt gtaaaaatgg aaataaccac ctttcaccaa tttcacctcg attttcggca
      721 acgaaaaata aatcagacct gccagcatga cgcaggcgag ggtgatgacg ctaatttgtc
      781 gcgaaagttt tattttgtaa gtttcgctgg cgaatgccaa caggcaggcc gcgaagaggc
      841 cggcggcgaa aagtcccgcg ccccagttca gcgcaatggt caatgcccca tgccggctga
      901 agaggcggtt cttgtgttct aaaaagaaag gtgattgatc gagccagaca tcctggcgca
      961 tttcggcggg cgaaagcgtc gccgttagtt tggtggccac gggcgaatcg ccgacccact
     1021 cacgcagcag gggatgaaca acgtttggtc gcgtgcagct ttcaactttg aaaagcggcg
     1081 cggtggcaga tgcgctgccg aagacgtaga ggtcaacact taacgacggg ctgtccattc
     1141 cggtcaggcg catcggataa accggctt
//
LOCUS       DATCBB010000781         1034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_480894, whole genome shotgun sequence.
ACCESSION   DATCBB010000781 DATCBB010000000
VERSION     DATCBB010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480894"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..>1034
                     /locus_tag="VII37_08660"
     CDS             97..>1034
                     /locus_tag="VII37_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5069848.1"
                     /translation="MDMQNIRLKVLLVPSMAALWLAGCMVGPRYHRPDATPQPPPPAY
                     KESPTQVQDPGPWKVAEPKDAMLRGKWWGIYNDSELNSLEDQLNINNQNIKQFFENLM
                     AARALVAQARSQLYPTIGINPSISRSQSSGNLGNSTPTTGTGGTGGTVTTTAGRQSSL
                     FSLPADVSWAPDLWGRIRSQIHAAQYNAQLSAADLENERLIEQASLASFLFELRGQDA
                     LRKIFDETIEADKKSVELTRARFETGIDNEISLVQAQQALESAEAAGANLGVARAQFE
                     HAIATLIGKPASDFSLPVKPLDHAPPAIPIGVPSQL"
BASE COUNT          237 a          288 c          285 g          224 t
ORIGIN      
        1 cactcgaccg cttgcggctt cgcatgctgg gaaaacgggg cgacacattt catccagcgg
       61 gtgcggcgcc ttccgcggcg ggttagtttg aaagttatgg atatgcagaa tattcgcctg
      121 aaagtcctcc tcgttccgtc aatggcggca ctctggcttg ccggatgcat ggtcggcccg
      181 cgatatcacc ggccggacgc gacgcctcaa ccgccgcctc ctgcatataa ggaatcgcct
      241 actcaggttc aggatcctgg cccatggaaa gtcgcggaac ctaaagatgc catgctgcgc
      301 gggaaatggt ggggaattta caacgactct gagctgaatt cgctggaaga ccagctgaac
      361 atcaacaatc aaaacattaa acaatttttc gagaatctca tggctgcgcg agctctggtt
      421 gcgcaggcac gttcgcaact ttaccccacg ataggaatca atccttctat ttcacggtcg
      481 caatcttccg gtaatctcgg aaattccacg ccgactacgg gaacgggagg aactggagga
      541 acagtcacaa cgacggccgg ccggcaatcc tcactctttt cgcttcctgc cgacgtttcc
      601 tgggcccctg atctgtgggg caggattcgc agccagattc atgccgctca atacaacgcg
      661 caattgagtg ctgcggacct ggaaaacgaa cgactgattg agcaagccag tctggcgagt
      721 tttttgttcg aactgcgcgg ccaggatgca ttgcggaaaa tcttcgatga gactattgaa
      781 gcggataaga aatcagtgga gttgacccgc gcgcgatttg agaccgggat tgataacgaa
      841 atttcgctgg tgcaagcaca acaagcattg gagagcgccg aagccgcggg tgcgaatctt
      901 ggtgtggcgc gcgcgcagtt cgaacacgcc atcgccacgc tcattggcaa gccggcctct
      961 gacttttcgc ttccggttaa accgctggac catgctccac ctgcgattcc cataggagtg
     1021 ccgtcgcaac tttt
//
LOCUS       DATCBB010000782         1077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_122954, whole genome shotgun sequence.
ACCESSION   DATCBB010000782 DATCBB010000000
VERSION     DATCBB010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122954"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VII37_08665"
     CDS             complement(<1..541)
                     /locus_tag="VII37_08665"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5069849.1"
                     /translation="MAKLTLMFEEKTVKEVSLGARPVGIGRSPDNDLTVDNLAVSNYH
                     ARVYVDSGKMVVEDLKSLNGTFVNEMRVERATLKHGDSIHVGKHHIKVDATGEAPAAY
                     GSERKAATPKINETMVLDTKMRREMMQQAAALGERTQLAAGRLRVPTLVVLGGKTDRH
                     EYTLTSKLTVIGKSHLATVR"
     gene            complement(551..>1077)
                     /locus_tag="VII37_08670"
     CDS             complement(551..>1077)
                     /locus_tag="VII37_08670"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY5069850.1"
                     /translation="PQQQGMGATLTAAWINEEKLSVVHVGDSRIYLLRSGVLQQLSSD
                     HSLVAEQVRRGILTPAEAEHSELQSVLMRALGTQPDVEVDAEEHILFPGDILLLCTDG
                     LTRMVAEQKIAAALQSEKDPGRAAEKLVALANENGGADNVTVVVVHLEPKEHGFLSWL
                     RHGAQKNAGKNGSA"
BASE COUNT          183 a          333 c          286 g          275 t
ORIGIN      
        1 agcggacggt ggcgaggtgc gatttaccta tgaccgtcaa tttgctggtc agcgtgtatt
       61 cgtgccggtc ggttttgccg ccgaggacca ccagcgtggg tactctcaaa cgtcccgccg
      121 ccaattgggt gcgctccccc agtgctgccg cttgttgcat catctcgcgg cgcattttgg
      181 tatccagaac catcgtttcg ttaattttcg gcgtagcggc tttgcgctcc gagccgtagg
      241 ccgcgggggc ttctccggtt gcgtcaacct tgatgtggtg cttgccgacg tggatggagt
      301 caccatgctt cagcgtggca cgctccacgc gcatttcgtt gacgaaggtg ccgttcaggc
      361 tcttgagatc ttccaccacc attttgccgg agtccacgta cacacgcgcg tgataattgg
      421 aaaccgcaag gttgtcgacc gtcagatcgt tgtcgggaga ccgcccgatt ccgacaggcc
      481 tcgcaccaag tgatacttct tttaccgtct tttcttcgaa catcagtgta agtttcgcca
      541 tttcacatcc tcatgctgag ccgtttttgc ccgcattttt ctgcgctccg tggcgcaacc
      601 acgacaaaaa tccgtgttcc ttcggctcca ggtggacaac aactaccgtt acgttatccg
      661 cgccgccatt ttcatttgcc agcgccacta gtttttccgc cgctcgcccg gggtcttttt
      721 ccgattgcag ggcggccgca attttttgtt ccgccaccat gcgcgtcaaa ccgtcagtgc
      781 acagcagcaa aatatcgccg gggaaaagga tatgctcctc ggcgtccacc tcgacgtctg
      841 gctgagttcc cagagcgcgc atcaggacac tctgcaactc gctgtgttcc gcttccgcgg
      901 gagtcaaaat tccgcgccgc acttgttcgg ccaccaggga gtgatcgctg ctgagctgtt
      961 gcaatactcc gctgcgcagc aggtatatcc ggctgtcacc cacgtgcacg acgctgagtt
     1021 tctcttcgtt gatccatgcc gccgttaacg tcgcgcccat gccttgctgt tgaggat
//
LOCUS       DATCBB010000783         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_220275, whole genome shotgun sequence.
ACCESSION   DATCBB010000783 DATCBB010000000
VERSION     DATCBB010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220275"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VII37_08675"
     CDS             <1..281
                     /locus_tag="VII37_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEY5069851.1"
                     /translation="CVTHPAGETKPHAQIDEVRSGGGYFSQNDLRVHFGIGKAEKIDL
                     LEIRWPSGLVEKVENIKPNQVIYVKEGQGIVRTMQFPEDKGSARRKSS"
     gene            417..>2183
                     /locus_tag="VII37_08680"
     CDS             417..>2183
                     /locus_tag="VII37_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069852.1"
                     /translation="MRPALRDLSIKNLALAIVFVFILGAMSHSAALAQKGESAKADTA
                     TDATAEYGRGVSALQRGDLAGARAAFEKVLRLVPNSAEGHNSLGWVLLAQGQTDEAIV
                     HFRAALRAKPDFSQAHINLSNALVQKGDRDGAVRQARDAVHEAPRDSESHHTLGRALA
                     FSGDNKNAVLEMRAASEIEPLRADLHDELGALLVQQSDAREANLQFEEAVRLDPRMAA
                     AHLHLGVLRLQEKSGDAGLSELRVAVQLEPQNPQSHYYLAKALQQTDQAEEAMGELQS
                     VVRLQPDFPEAQNELGLLLQHSGNANGAVQAFREAVRLQPENPDAHSNLGLALLQVGD
                     GDGAIAEFKSAVRLRPGDTSYRANLGVAYLQKTDFDAAIAQFQSALADAPNDATLHYD
                     LGLALKLKDKLPEGIAELRKAAELNPEQADAHYTLGVTLWQQGELPAAESELRAAVRA
                     KPDYAEAFYTLGTVLKQQEKWQDAAVALREAIRLQPDFAGAHTTLAVVLRQLGDTQGA
                     ADESRAGAAMAKKTTDMQAAVFATNSGARLMNAGDLDAAISQFRAAIAATPGYAPAHY
                     QLGLALQRKGEREESGRELKKAA"
BASE COUNT          500 a          528 c          700 g          455 t
ORIGIN      
        1 gttgcgttac acatcccgcg ggagaaacga aaccgcacgc gcagatcgat gaagtgcgca
       61 gtggcggcgg ctatttctcg cagaatgact tgcgggtgca cttcgggatt gggaaagctg
      121 aaaaaattga tctgctggaa attcgctggc cgagcgggtt ggtagaaaaa gtggaaaata
      181 tcaagcccaa ccaggttatt tatgtgaagg aagggcaggg aattgtgcgg acgatgcaat
      241 ttccggaaga taaaggttcg gcgaggcgta aatcgtccta gcctcccgag gtcgctgttt
      301 gctcgactcg caaaacgcga gcctccgcgc ggcatttcgg aaaccaagca tcctgccgct
      361 atcgcgtgag ctgcgtgtta acatttcttt ctacagctta agcattctct acgattatgc
      421 gtcctgcatt acgagacctg tcgattaaaa acctcgcgct cgcgatagtt ttcgtcttta
      481 ttctgggtgc aatgtcgcat agtgcagcgt tggcgcaaaa aggcgagagc gccaaggcag
      541 atacggcgac agacgcaacc gctgagtacg ggcggggagt gagtgcattg cagcgtggcg
      601 atcttgcggg cgcgcgagca gcgtttgaaa aagttctgcg attagtgccg aacagtgcgg
      661 aagggcacaa ctcgttgggt tgggtgttgc tggcgcaggg gcaaactgac gaagccatcg
      721 tgcattttcg agctgcactt cgcgcgaaac ctgatttttc gcaggcgcat ataaatttat
      781 ccaacgcgtt ggtgcagaag ggcgatcgtg atggcgcggt gcgccaggca cgcgatgccg
      841 tgcacgaagc gccgcgagat tcagaatctc atcacacgct cggacgagct cttgcgttct
      901 cgggagataa caaaaatgct gtgctcgaga tgcgagctgc cagtgagatt gaacctttgc
      961 gggctgattt gcacgatgaa ctcggtgcgc tgctggtgca gcaatccgat gccagggaag
     1021 ctaatctgca attcgaagaa gcggtgcggc ttgatccgcg aatggcggcg gcgcacctgc
     1081 atctgggggt tttaaggtta caggaaaaat caggagatgc aggtctatcg gagctgcgcg
     1141 tggctgtgca gttagagcca caaaatccgc agtcgcatta ctacctggcc aaggcactgc
     1201 aacaaacgga ccaagcggaa gaagcaatgg gcgaactaca gagcgtggtg cgattacagc
     1261 ctgattttcc ggaggcgcag aacgaattgg gtttattgct gcaacattcg ggaaatgcga
     1321 atggcgcggt gcaggcgttt cgggaggcgg tgcgattgca accggaaaat cctgatgctc
     1381 atagcaatct gggattagct cttttgcaag tgggcgatgg cgatggtgcg atcgcggagt
     1441 tcaaatcggc ggttcgttta cggcccgggg acaccagtta ccgcgcgaac cttggcgtcg
     1501 cctatttgca aaagacggat ttcgacgcag ccatcgcgca atttcagtcc gcgctcgcgg
     1561 atgcgcccaa tgatgcgacg ctgcactacg atctggggct agcgttgaag ttgaaggata
     1621 aacttccaga aggaatcgcc gagctgcgca aagctgcgga gctaaatccc gagcaggctg
     1681 atgctcatta cacgctggga gtcacacttt ggcagcaggg cgaattgccg gcggcggaaa
     1741 gcgaacttcg cgctgctgtt cgtgccaagc ctgactatgc ggaggctttc tacacgcttg
     1801 gtacagtgtt gaagcagcag gagaagtggc aggatgctgc tgtcgcgttg cgtgaagcga
     1861 ttcggctgca gccggacttt gctggggcgc acacgacctt ggcagtggtg cttcggcaac
     1921 tcggtgatac gcaaggcgct gctgacgaga gccgcgcggg tgcggccatg gcgaagaaga
     1981 ctactgatat gcaggctgcg gtgttcgcga caaattctgg cgcgcgcttg atgaatgcag
     2041 gggacctgga cgccgctatc tcacagtttc gcgctgcgat tgcggctaca ccgggatatg
     2101 cgcccgccca ttatcaattg ggattggcgt tacaacgaaa aggcgagcgt gaagagtcgg
     2161 gcagagagtt aaagaaggcg gcg
//
LOCUS       DATCBB010000784         1038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_25192, whole genome shotgun sequence.
ACCESSION   DATCBB010000784 DATCBB010000000
VERSION     DATCBB010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25192"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..858)
                     /locus_tag="VII37_08685"
     CDS             complement(232..858)
                     /locus_tag="VII37_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069853.1"
                     /translation="MMDQMMTMDEYIGGYYYSAPYPLNGALRRILGYSRSDTYRVGGR
                     LQHLNNSPDSEFAAEDFAAFKAGKPQNTFTYFLQVEAEQIRLIREFKARGAAEPNVST
                     NALLMARTSTLVFAHLWKHAALTPFFLWRGAFFAFPPLAVAFAWSLRRRSQQLTLLTL
                     TALGMVLFDALVSFFTPRYSLPAAPLALCAAAALCVALFGRFRPLRKL"
BASE COUNT          242 a          315 c          276 g          205 t
ORIGIN      
        1 cgctacggcg cgcatctcaa tacgctgccg ggaaattcct ccgccgccgt gacatttgtg
       61 ctcgcggcgc tgctctcctc gctaatcacg tacccgctaa atgcacgatt cgtgttccgc
      121 cgcgatcgct gagcgtgtgg gacttgtcga tgatgagcag caagttgctc gccataccca
      181 atcgtcgttg cgactccacc gcaacttcac agccgttgag cagtgggtgg atcagagctt
      241 tcttagcggc cgaaaccgac cgaacagtgc gacgcacaaa gctgccgccg cacacagcgc
      301 cagcggcgcg gccggcaggc tgtaacgtgg cgtaaagaat gagaccagcg catcaaacaa
      361 aaccatcccc agcgcggtta gggtaagtag cgtgagctgt tgacttctgc gccgcaacga
      421 ccaggcaaac gcgacggcca gcgggggaaa ggcaaagaat gcgccgcgcc acagaaagaa
      481 cggcgtgaga gccgcgtgtt tccacagatg ggcaaacacc agcgttgagg tgcgagccat
      541 taataatgca ttggtgctga catttggttc cgcagctccc ctcgccttaa actcgcggat
      601 caatctgatt tgctctgcct cgacttgtaa gaaatacgtg aatgtatttt gcggcttgcc
      661 cgctttaaaa gccgcaaaat cttccgcggc aaactccgag tccggcgaat tattcaagtg
      721 ctgcaaacgt ccgcccacgc ggtaggtatc cgagcgcgaa taaccaagta ttcgccgcag
      781 cgctccgttc agcggataag gcgccgagta gtaataccca ccgatatatt catccatcgt
      841 catcatctgg tccatcacgc cgcggatata gaggacttca cctccccgcc cggcgatact
      901 aatttatatc agctcgtcga accggcaacc gctatgcaga tcacggcccg gcgggcgtcc
      961 cgccgctccg acactgagaa gtgcgttctt gcctaaagct gcataagatg atgtcatttt
     1021 gcaatcaccc gaacggaa
//
LOCUS       DATCBB010000785         1976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_57750, whole genome shotgun sequence.
ACCESSION   DATCBB010000785 DATCBB010000000
VERSION     DATCBB010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57750"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VII37_08690"
     CDS             complement(<1..1864)
                     /locus_tag="VII37_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY5069854.1"
                     /translation="MASIVVLLFSFTLPCCSKAQWNPLNPVSHVKRDDGGVTLTMHAG
                     TLRIQVASESIVRVTYSATSAFPDSPRFMITKTSWPTAQWDMKSTDKDITVSTARLKV
                     TITRANGTILFSDASTKKLFQDYDRTLTPVEVNGKKTYRAEMFSNLWDSTEAFYGLGQ
                     HQAGVWNYHGEDVELSQDNTNISVPMFLSSNGYGIFWNNASRSRFNNRFLHALYLSSE
                     VADSVDYYFLFGPEFDKIIGAYRDMTGAAPMFGKWAYGFWQCKNKYESQAEILGVAHK
                     YRELRIPVDNIVQDWFWWQSMGEPVFNKNYPDPPRMMADLHKNNFHVMFSFWPFFKAG
                     SGVYEEMDKRGFFIDRTKAAAFHPVGQALYDPFNPEARKYYWQLLDKGLFRIGADAWW
                     LDTDEPETEGRETNVLVNNKVALGSGARYANLFPLMTTEAVYQGQRGASSEKRVFILS
                     RSAAAGIQRNSVAAWSGDVESNWLSFARQIPAGLNFSLSGIPYWTTDIGGFIIGNPDD
                     PAYRELFIRWFEYGVFCPIFRLHGTRTTNQNELWSYGTEAQEILTRYDRLRYRLMPYI
                     YSLAWRTTSESYTPMRPLAMDFAGDTRALNTGDQFMFGPALLVNPVTEPGARKRH"
BASE COUNT          416 a          587 c          538 g          435 t
ORIGIN      
        1 ggtgccgttt cctcgccccc ggctcagtca ccggattcac cagcagcgcc ggcccaaaca
       61 taaattgatc gccggtattc agcgcgcgcg tatccccggc gaaatccatc gccagcggcc
      121 gcatcggcgt gtagctttcg ctggtcgtcc tccacgccag cgaatagatg tacggcatca
      181 gccggtagcg cagccggtcg taacgagtga gaatttcctg cgcctccgtc ccgtacgacc
      241 acaactcgtt ctgattcgtg gtgcgcgtgc cgtgcagccg gaaaatcgga cagaatactc
      301 cgtactcaaa ccagcgaatg aaaagctcgc gatacgccgg gtcgtccgga ttgccgatga
      361 tgaatcctcc aatatccgtc gtccagtaag gaatccccga aagcgaaaaa ttcagtcccg
      421 cgggaatttg tcgcgcaaag ctcaaccaat tggattccac gtcgcccgac cacgccgcca
      481 cagagttgcg ctggatcccc gcagccgccg aacgcgaaag aataaaaacg cgcttctcgc
      541 tggacgcccc gcgctggccc tggtacaccg cttcggtggt catcagcgga aacaggttgg
      601 catagcgcgc gccgctgccg agagccacct tgttgttcac cagaacgttg gtctcgcggc
      661 cttccgtttc cggctcgtcg gtatccagcc accaggcatc ggcgccgatg cggaataatc
      721 ctttgtccag caattgccaa taatattttc tggcttccgg gttaaacgga tcgtatagag
      781 cctgccccac cgggtgaaat gccgcagcct tggtgcggtc gatgaaaaat ccacgcttgt
      841 ccatctcttc gtaaacgccg ctgccggctt taaaaaatgg ccagaacgaa aacatcacgt
      901 ggaaattatt cttgtgcagg tccgccatca ttctcggcgg gtcgggataa ttcttgttga
      961 acactggttc gcccatggac tgccaccaga accagtcctg cacgatgttg tccaccggga
     1021 tgcgcagctc gcggtatttg tgcgccacgc ccagaatttc cgcctgggat tcgtacttgt
     1081 tcttgcattg ccagaatccg tacgcccact tgccgaacat cggcgccgcc ccggtcatgt
     1141 cccggtaagc gccgatgatc ttgtcgaact ccggcccgaa caagaaatag tagtcgacgg
     1201 aatccgcgac ttcagagctc aaatatagtg cgtgtaggaa gcggttgtta aagcggcttc
     1261 gggaagcgtt gttccagaaa atgccatagc cattgctgga gagaaacatg ggcacggaga
     1321 tgttggtatt gtcctgcgag agctccacgt cttcgccgtg atagttccac acacccgcct
     1381 gatgctggcc gaggccataa aaggcttccg tggagtccca caggttggaa aacatttccg
     1441 cgcgatatgt tttctttccg tttacttcta ccggcgtcag cgttcggtcg taatcctgaa
     1501 atagcttctt cgtgctggcg tcgctgaaga gaattgttcc gttcgcgcgc gtgatggtca
     1561 ctttcagccg agccgtcgac accgtgatgt ctttatccgt tgatttcatg tcccactgcg
     1621 ccgtcggcca actggtcttg gtgatcatga atcgcggcga atcgggaaat gcgctcgtcg
     1681 ccgaataggt cacgcgtacg atcgattcgc tggccacctg aatgcgcagc gttccggcgt
     1741 gcatcgtcaa agtgacgcca ccgtcgtcgc gttttacgtg actgactgga ttgagcgggt
     1801 tccactgcgc cttgctgcag cacggaagcg tgaaggaaaa cagcaagaca acaatcgaag
     1861 ccattctgtg cggcgtaatc gtttgcggca tgggaccccc gaccttagaa taagtcggcg
     1921 ggattatacg cgaccacccc gtgcgtcggc tgaagaattg tgcagtccgc cgctat
//
LOCUS       DATCBB010000786         5199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_414731, whole genome shotgun sequence.
ACCESSION   DATCBB010000786 DATCBB010000000
VERSION     DATCBB010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414731"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VII37_08695"
     CDS             complement(<1..880)
                     /locus_tag="VII37_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5069855.1"
                     /translation="MAEEAKGAVRALEAPERRDRVLIVEDEDNARKGYEQLLQRWGCE
                     VLGVSNAEDALAKFATFQPDALIADVELPGMNGLDLLKQLGPELHDVPAIIITGRGSE
                     ERAVAAIEAGAFWYIEKPLKGSVLHALLDRALSRARDARQLVVLQRQLRESGRLGDLV
                     GGSKPMQDVMRLVEMAAPSSASVLITGETGSGKEIVARTIHKLSPRAGGPFVAINCSA
                     IPETLMESEIFGHERGAFTGAAERRIGCFELADAGTLLLDEIGEMPAPTQAKLLRVLE
                     DRKVRRLGSKSETPVDV"
     gene            complement(1057..1797)
                     /locus_tag="VII37_08700"
     CDS             complement(1057..1797)
                     /locus_tag="VII37_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069856.1"
                     /translation="MTRLAAYAKATCVLITTILLAATICSAGPKEIRQPLQQYNGQSA
                     PEPQQADGRRHGLPPETYAVAPGTKFLVVLEQDLTSKGTEENAKFLVRTVEPLEAGRG
                     IYLPPGAEIHGHVSRIESAGVTGRAKIWLTFDDIYTKFGKLPIVAEVVSVPGDHSVKP
                     AGPSQEGLIEGRSSTQQDAAQAAAAGAAGGAVQGVKDKDKKEAAQGAALGALTAYLVE
                     SGRGHELNLPKGAKLELELERALYLLKE"
     gene            complement(1962..2636)
                     /locus_tag="VII37_08705"
     CDS             complement(1962..2636)
                     /locus_tag="VII37_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5069857.1"
                     /translation="MKRLAREVVACRLCPRLVEYRERVAREKRRAYLDWEYWGRPVPG
                     FGDPNARLLIVGLAPAAHGGNRTGRVFTGDRSGDFLFAALYEAGFANQPTSVRRDDGL
                     KLIDSYITATARCAPPDNKPLPGEILNCRGYMEREIRIMEPRAVLALGRIAWDAYLEV
                     LKQQGLITSRTPYRFAHGAEIQLPPPAPRLFGVYHPSQQNTQTGKVTPPMYAEVLRAI
                     RKFLGD"
     gene            complement(2651..3433)
                     /locus_tag="VII37_08710"
     CDS             complement(2651..3433)
                     /locus_tag="VII37_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5069858.1"
                     /translation="MGYREEYGPLLDSLVQEGDVIAMRYFRAVEMRVDRKGDGSAVTQ
                     ADRGVEEMARARVAASGLALDVLGEEMGGWDMEAASSSDRARLIIDPIDGTEEFSRGI
                     PTFGTLLGIEHRGEIVAGMVSAPALGTRWFAFRGEGAFRDGKKIHVSSVSKLSEAMIF
                     TTGTGPKKNAFDRAKLRRLHDAVRNSRAMGGFWQHMLVAEGAIDAAVDWTSMPWDLAP
                     LGLIVEEAGGKSTNLHGERTIYGGQFVSSNGIIHHEVLGLLT"
     gene            3543..4412
                     /gene="trpC"
                     /locus_tag="VII37_08715"
     CDS             3543..4412
                     /gene="trpC"
                     /locus_tag="VII37_08715"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY5069859.1"
                     /translation="MSAHTNTGTVLDRILEARRAEVEHRKCVLPETALKYGAKAASPV
                     RDFYSALSRDGLNIIAELKPASPSRGIIREPFDAPELAKGLSQAGAAALSVLTEGEYF
                     HGSLKNLRDARKAMGLPVLRKDFIFDPWQVWEARANDADSYLLIVAVLSDSLLRDLIT
                     LGREIGMEPLIEVHTAAELERALAASAKIIGVNNRDLKTMAVHIETSLQLIEQIPENC
                     VAVSESGIRNHGDLLKMRQAGFDAFLVGEHLMSASDPGAALVELLGQPRVTREQFDAA
                     AAATDAKNGTWQN"
     gene            4416..5084
                     /locus_tag="VII37_08720"
     CDS             4416..5084
                     /locus_tag="VII37_08720"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY5069860.1"
                     /translation="MTRIKICGITNLEDARAACEAGVDAIGLNFYSRSPRALSIEIAG
                     NLRASIPSSVQVFGVFVNAEADEVAKILRDVHLDALQLHGDESPATVAQLARIAPVFK
                     ALRVGPDFTAATLENYPGVSGFLFDTADARPGQYGGTGRLADWGVAQQAALSHKVILA
                     GGLDAENVAAAISQVRPYGVDVASGVESSPGAKDHHQLCDFVREARRVDQELNSPVEK
                     LRAR"
BASE COUNT         1052 a         1548 c         1400 g         1199 t
ORIGIN      
        1 gcacgtcgac cggcgtttcg cttttgctcc cgaggcggcg aactttgcgg tcttccagta
       61 cacgcaataa tttggcttgt gtaggcgcgg gcatttcgcc gatttcatcc agcagcaggg
      121 ttccagcgtc ggccaattca aaacagccga tgcgtctttc ggccgcaccg gtaaacgcgc
      181 cccgctcatg accaaaaatt tcgctttcca tcagcgtttc gggaattgcc gagcaattga
      241 tggcaacaaa tgggccgccg gcacgcggcg acaatttgtg aatggtgcgg gccacgattt
      301 ccttgcccga tccggtttcc ccggtgatca acacggaggc cgaactgggc gctgccattt
      361 cgactagccg catcacgtct tgcatcggct tggaaccgcc aaccagatcg cccaatcttc
      421 cggattcgcg tagctgtctt tgcaacacca ccagctgccg ggcatctcgc gcgcggctca
      481 atgcgcgatc caacaatgcg tgcagcaccg aaccctttag tggtttttcg atgtaccaga
      541 aagctccggc ttcgatggcc gcgaccgcgc gctcttcgct gcctcgcccg gtgataatga
      601 tcgccggaac gtcgtgaagc tccggaccaa gctgcttgag caggtccaat ccattcatgc
      661 ccggcagttc gacgtcagca atcagcgcat cgggctggaa agttgcgaat tttgccagcg
      721 cgtcttccgc gttgctcacg cccagaactt cgcaccccca gcgttgcaac aattgttcgt
      781 aacccttgcg tgcgttgtcc tcgtcttcga caatcagaac gcggtcgcgg cgctcgggcg
      841 cttcgagagc gcgaaccgcg cccttcgctt cttccgccat gtataaaaac ctctccgcct
      901 atcgtgattt tggctgtcta gaaagccgct tgactgggca atctcgactc taacaagccc
      961 acaggtgctt gccaagtttt gataactttt ttcttagacc acttgtgaaa tacactgtat
     1021 gttcatgtca ttactatgtc gctgcgcggg gacaagtcat tctttcagca aatacagcgc
     1081 tctttccaac tctaactcca attttgcacc cttcggcaaa ttcagttcgt gtccgcgccc
     1141 ggattccacg agataagccg tcagggcccc gagggccgcg ccttgagcgg cttctttctt
     1201 gtctttgtcc ttcactcctt gcaccgcgcc tcccgctgcg ccggccgctg cggcttgggc
     1261 agcatcctgc tgcgtgctgc tgcgtccttc gattaagcct tcttggctgg gcccagcggg
     1321 cttaacgctg tgatcgcctg gaacgcttac tacctctgcg acgatgggca gcttgccgaa
     1381 ttttgtgtaa atgtcatcga aggtcagcca aatttttgcg cgtccggtaa cgccggccga
     1441 ttcgatgcgg ctcacgtggc cgtgaatttc tgcgccaggg ggcagataga tacctcgacc
     1501 ggcctccaga ggctcgacag tcctcactaa aaatttggcg ttttcttccg tacccttgga
     1561 agtgaggtct tgctccagaa ccaccaggaa tttcgtccct ggggcgaccg cgtaagtctc
     1621 tggcggcaaa ccatgcctgc gtccgtccgc ttgctggggt tcaggcgcgg attgtccgtt
     1681 gtactgctga agcggttgcc gaatttcttt cggacctgcg gaacaaatcg tggccgcgag
     1741 caatatggtg gtgatcagga cgcaggtggc cttggcgtac gctgcaagtc gagtcatgca
     1801 ttctctcccc agattttatt tccgccattt atcgaataat cgttatgaat atttgatgcg
     1861 cagattgtta gatgcgacct gggagccaac gcgtatttca agggtatttc ttcatctgtg
     1921 ccaatgcaag agcgggtatt tattcgcaga ccggctcgga ttcaatcgcc gagaaacttt
     1981 ctaatggccc gtaaaacctc cgcatacatg ggtggcgtaa ctttaccggt ctgcgtgttt
     2041 tgttggctgg gatggtatac gccgaacaat cggggcgcgg gcggcggaag ttggatctca
     2101 gcgccatgcg caaagcgata aggcgtgcgc gaagtaatca gtccttgctg cttcagcact
     2161 tccaggtaag catcccaggc aatgcggccc agagccagaa cggctcgcgg ttccatgatg
     2221 cgaatttcgc gctccatgta tccgcgacag ttcaagattt cgcctggcaa cggtttgttg
     2281 tctggcggag cgcaccgcgc cgtagcggtg atgtaactat caatcaactt taaaccgtca
     2341 tcgcggcgca cagacgtcgg ctggttcgcg aatccagctt cgtaaagtgc cgcaaaaaga
     2401 aaatcgccag atcggtcgcc ggtaaatacg cggccggtgc gatttcctcc gtgcgccgcg
     2461 ggagcaagcc cgacgatcag caacctcgcg ttggggtcgc cgaatcccgg cactggcctg
     2521 ccccaatatt cccagtccag ataagcgcgt cgcttttcac gcgctacgcg ttcgcgatat
     2581 tcaacaagtc gcgggcacaa gcggcacgcc accacctcgc gcgccaggcg tttcaaagca
     2641 gctccccggt tcacgtcaaa agtccaagaa cctcatggtg aattattccg ttgctgctga
     2701 cgaattgccc gccgtaaatc gttcgctcgc catggagatt tgtcgatttg ccgccggctt
     2761 cctccacgat taaacccagg ggggccaggt cccaaggcat ggaggtccag tcgacggcgg
     2821 cgtcgatcgc gccttcggca accaacatgt gttgccaaaa gccccccatt gcacggctgt
     2881 ttcgcaccgc atcgtgcagt cggcgtagct tcgcgcggtc gaatgcattt ttcttggggc
     2941 cagtcccagt ggtgaaaatc atcgcctcac tgagtttcga cacgcttgat acgtgaatct
     3001 tcttgccgtc gcgaaaagcg ccctcgccgc gaaacgcgaa ccatctggtg cccaacgcag
     3061 gagcgctcac catccccgca acaatttctc cgcgatgttc aatgccaagc aacgtcccaa
     3121 atgtaggtat gccgcgcgag aattcttcag tgccatcaat tgggtcaata attaatctcg
     3181 cgcgatcgct ggaactggca gcttccatgt cccacccgcc catttcctcg cccaaaacat
     3241 cgagagccaa tccgctggcg gctactcttg cacgcgccat ttcctctacc ccgcgatcgg
     3301 cctgagttac tgcagagccg tcgcctttgc ggtccacgcg catctccacg gcgcgaaaat
     3361 atcgcatggc gatgacgtcg ccctcctgca cgagggaatc gagcaacggt ccgtattctt
     3421 cgcgatatcc catcatttct ccgtttggtc ctcaaacctt gacacggcct gcccatttga
     3481 caggctggcc tgtctcgatt tgacaggcca gccgcagcta cctagcatac aaacttaccc
     3541 ttttgagcgc tcacaccaac accgggacag tgctggaccg cattctcgaa gctcgtcgcg
     3601 ctgaggtgga gcatcgtaaa tgcgtgctgc ccgaaacggc gctgaaatac ggtgccaaag
     3661 cggcctcgcc cgtacgtgat ttctattccg cgttatcacg cgacggcctg aacattatcg
     3721 cagagctgaa acccgcatct ccttcgcgcg ggatcattcg cgagccattt gatgctccgg
     3781 aactggccaa aggtctgtca caagcgggcg ccgccgcact ttctgtgctt accgaaggtg
     3841 aatattttca tggctcgctt aaaaacctcc gcgatgcgcg aaaagccatg ggcctgcccg
     3901 tactgcgcaa ggattttatt tttgaccctt ggcaagtgtg ggaagcgcgg gccaatgatg
     3961 cggacagcta tctcctgatc gtcgcggtgt taagcgattc tctgttgcgc gatttaatta
     4021 cccttggccg cgaaatcggc atggaaccat tgatcgaggt gcacactgcg gcggagcttg
     4081 aacgagctct agccgcttcc gcgaaaatca tcggggtgaa caatcgcgat ttgaaaacca
     4141 tggcggtgca catcgaaact tcactgcagc ttatcgaaca gattccagaa aattgcgtgg
     4201 ccgtcagcga atccggcatt cgcaatcacg gggatctctt aaagatgcgt caggccggct
     4261 tcgatgcatt cctcgtcggc gagcatctta tgagtgcttc cgatccggga gcggcgctcg
     4321 tcgaattgct gggacaacca cgagtgacca gagagcaatt cgatgcggcg gccgcggcta
     4381 ccgacgcgaa gaacggtact tggcaaaact aattcatgac ccgaataaaa atttgtggga
     4441 ttaccaatct cgaggatgcg cgtgccgcct gtgaggccgg cgtggacgct attggtctga
     4501 atttttattc ccgaagccct cgcgcgctgt ccatcgaaat agccggtaat ctacgcgcct
     4561 cgattccctc aagcgttcag gtttttggag tctttgtgaa cgcggaagcc gatgaggtcg
     4621 caaaaatatt acgcgatgtg catctcgatg cgttgcaatt acacggcgat gaatctcccg
     4681 ccacggttgc ccagttggct cggatagctc cggtctttaa agcccttagg gtcggccctg
     4741 attttaccgc tgctacgctc gaaaattatc caggagtttc tggtttttta ttcgacaccg
     4801 ccgatgcccg acctggtcaa tacggcggaa ccgggcggct cgcggactgg ggagtcgcgc
     4861 agcaggcggc gctaagtcac aaagtgatcc tggccggcgg attggatgcc gagaatgtgg
     4921 cggcggctat ttcgcaagtc cgcccgtacg gcgtcgatgt cgctagcggc gtcgaatcct
     4981 cgcccggtgc aaaagatcac caccagttgt gcgactttgt tcgcgaagca cgtcgagtcg
     5041 atcaggaact aaattcgcca gtggagaaat tgcgcgccag atgagctcca tcctcaaatc
     5101 gttgagcttt tcgcccgggc gttttggccc gtacggcggc cggtacgtcc ctgaaacctt
     5161 gatggtgccg ctcttcgaac tggaacgcgc ctatgaact
//
LOCUS       DATCBB010000787         1440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_349506, whole genome shotgun sequence.
ACCESSION   DATCBB010000787 DATCBB010000000
VERSION     DATCBB010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349506"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..769
                     /locus_tag="VII37_08725"
     CDS             395..769
                     /locus_tag="VII37_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5069861.1"
                     /translation="MFQVDANHPTPLYAQLERSIRFAIATGRLRIGDQLPTVRQLAVD
                     LRINANTVAKVYAGLERASVVETRRGVGTFVSARPNEAASRRDRDRHLRELTDHFITE
                     THTRGFSIDDVIEHLESRRQKK"
     gene            820..>1440
                     /locus_tag="VII37_08730"
     CDS             820..>1440
                     /locus_tag="VII37_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5069862.1"
                     /translation="MANIQNFETRSNLPVNSPRVNFVQAVAFLTPAALGALATLMTLN
                     PGGVIVGVLLGLLLSQSPKIARQWERAVVLRLGKFIGLRGPGLFWIIPFVDNISTWID
                     QRVITTAFAAEETLTSDTVPVNVDAVLFWVVYDPEKAALEVQNYPAAVSWAAQTALRD
                     IIGRTSLSDLLRGRERIEEELQKLIDERSNPWGVTVQSVEMRDVIIP"
BASE COUNT          306 a          380 c          424 g          330 t
ORIGIN      
        1 gttccgccga cagccaatgc cttcatcctg ttttctttcg caatcccgcc catgtatggc
       61 cagagtctcg attccaactc cgggtcgatg aagggatagc gctccttcgt gctgaagata
      121 tagtggacca ggttgcttac gtaagagtgt ggcatggtct cgcttccttt ttcagccgtc
      181 cgctttgcgg actagttgat gttttctgcc ggaccccagc gataaatcgc tgggctattt
      241 tcaagcgtcc gctgcgcgga ctcaggtggt tgactccgtc ggcaaggtaa cgccttttcg
      301 gctatttgaa tacgtaaaga tttttgcaac tgaacgggta aaaaactcct acagaacttt
      361 acttgtccca atgtcgtagt acaatagtgc aacgatgttt caagtcgacg ccaatcatcc
      421 gactccgctt tacgctcaac tggaaaggtc cattcgcttt gcaattgcga ctgggcggct
      481 gcggatcggc gatcagttgc caaccgtgcg gcagttggca gtggatttgc ggattaacgc
      541 aaataccgtg gccaaagtct atgccgggct ggagcgcgcc agcgtagtgg agacacgcag
      601 aggcgtcggg acttttgtta gtgcgcgacc gaacgaggct gcgagccgcc gcgatcgcga
      661 ccggcacctg cgcgagttga cggatcattt cattacggaa acacacacgc gcggcttttc
      721 gattgacgat gtgattgagc atctggaaag cagacgccag aaaaaatagc gcaagctgac
      781 agcttgcgct acaggcacaa gaaggagata aagacggtta tggctaacat tcaaaatttt
      841 gaaaccagga gcaacttgcc ggtgaattcg ccccgggtta actttgtcca ggcggttgcg
      901 tttctgacgc cggcggcgct cggcgcgctc gcgacgctga tgaccctcaa tccgggcggt
      961 gtgatcgtgg gcgtgttgct ggggttgcta ctgtcccagt caccgaagat cgcgcggcaa
     1021 tgggagcgcg cggtagtgct gcgcctggga aagtttatcg ggctgcgcgg tccgggactc
     1081 ttctggatca ttccgttcgt cgataacatt tccacctgga tcgatcagcg cgtgatcaca
     1141 actgctttcg cggcggaaga aacgttgacc agcgacaccg tgccggtaaa cgtcgatgcg
     1201 gtgttgttct gggtagttta cgatccggaa aaagccgcgc tcgaggtgca aaactatccc
     1261 gcggcggtta gctgggccgc ccagacggcg ctgcgcgata tcatcggccg gacgtcactc
     1321 agcgacctgt tgcgtggccg cgaacgtatc gaagaagaat tgcagaagct aatcgatgaa
     1381 cgctcgaatc cctggggtgt gaccgtgcaa tcggtcgaga tgcgtgacgt gattatcccc
//
LOCUS       DATCBB010000788         1426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_89745, whole genome shotgun sequence.
ACCESSION   DATCBB010000788 DATCBB010000000
VERSION     DATCBB010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89745"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..>1426
                     /locus_tag="VII37_08735"
     CDS             464..>1426
                     /locus_tag="VII37_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY5069863.1"
                     /translation="MPTGALDAITDVAGVEVGQTTIIRGDNIRTGVTAVLPHPSNLYR
                     EKVPGAIFVGNGFGKLVGSTQVEEMGDIETPILLTSTTSVPLVADALISFMLALPGNE
                     DVLSINPVVGETNDGYLSDIRGRHITPEDVFAAVKNAKGGPVEEGAVGAGTGTVVFGW
                     KGGIGTASRHLPANLGGYTVGVLVQTNFGGVLTIAGAPVGQELGQYYLRKELQQAGSG
                     KDKADGSCMIVVATDAPLDARNLKRLAVRALLGVARTGSSGSNGSGDYAIAFSTAAQV
                     RIHANDKAPTRHVEVMSNDAMSPLFQAAIETTEEAVYNSMFKATT"
BASE COUNT          300 a          334 c          484 g          308 t
ORIGIN      
        1 cgtgaggctt tcaagtaaag cgcacgcact tccgatgatg gatcgcccga attttggatg
       61 cttgaggtgt ggcgggttta gatcgcgaga tcagaaaaat tgggaacaac caacttcggg
      121 actttaggcg cagcggtgag gatcagtacg aagaatatgc tcgagcagcg agtaacgaac
      181 gttgccattt cggccttcca atggcggaag cttactacat atggctgagt gtctggtggg
      241 agttagcggg catttggcgt acacagtgat atagactgcg ggtcggatgg atggaggaaa
      301 ttctttgagg ttctgcgttg cgattttatt cttcctgagt actgttgcgt tggctggtcg
      361 tgaacctgcc cccgcaaaag cgatttttgc attcgctgcc gcacaacgca cgactcctaa
      421 tgcgcggccc cgcgcgtccg agttggggct gaaggttggg atcttgccga ctggggcgct
      481 cgatgcgatt accgatgttg ccggtgttga agttgggcag acgacgatta ttcgcggcga
      541 caacatccgc accggggtga ccgcggtgct gccacatccg agcaatcttt atcgcgagaa
      601 agtgcctggg gcgatttttg tgggcaatgg ttttggaaaa ctggtggggt caacacaagt
      661 ggaggaaatg ggcgacatcg aaacgcccat cctgcttacg tccaccacaa gcgtgccgct
      721 ggtggcggac gctttgatca gcttcatgct ggcgttgccg ggtaatgagg atgtgctttc
      781 tatcaatccg gtggtgggcg aaacgaatga cggatatttg agcgacattc gcgggcgcca
      841 cattacaccc gaggatgtgt tcgccgcggt taagaacgca aagggcggtc ccgtggagga
      901 aggcgcagtt ggcgcgggca ccgggacggt ggtgttcggg tggaaaggcg gcatcgggac
      961 tgcgtcgcgc caccttccgg ccaatcttgg tggatacacg gtgggtgtcc tggtgcagac
     1021 taattttggt ggcgtgctca cgattgcggg cgctccggtg ggccaggaac tggggcaata
     1081 ctacctgcga aaagaattac agcaggccgg cagcgggaaa gataaggccg atggttcatg
     1141 catgatcgtg gtcgcgacgg acgcgcctct ggatgcgcgc aacttgaaga gactggcggt
     1201 gagagcgttg ctgggcgttg cgcgcacggg gagttcgggc tcaaacggta gtggagatta
     1261 cgccattgca ttctcgacgg cggcgcaggt gcgcattcat gccaatgaca aagcgccgac
     1321 gcggcacgtg gaagtgatga gcaatgacgc gatgtcgccg ctgtttcagg cggcgatcga
     1381 aacgactgag gaagcggtgt acaactcgat gtttaaggcg acgaca
//
LOCUS       DATCBB010000789         1379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_316837, whole genome shotgun sequence.
ACCESSION   DATCBB010000789 DATCBB010000000
VERSION     DATCBB010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316837"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1022
                     /locus_tag="VII37_08740"
     CDS             171..1022
                     /locus_tag="VII37_08740"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY5069864.1"
                     /translation="MSNCDQFREMYEAYALGALDPSERAALEAHLATGCAECLKAVAE
                     ARWLVAQLAYLAPEASTSDMLKGRLMQTVRSEAKSSVPASTGAKTRRASGVPYWLSAG
                     IAALLLLTLYFAWDAHRLREELRAANEQNQRNQQLLASTQNEHAVSQQELTAMQEQMG
                     KMHRETAILTDPASVKITLAAQKIQAPQMVAMWHAKYGIVLAGQKVPMPSGNRVLQLW
                     LIPKAPGGKPMPSMTVRPDANGNFVILVANPPAAGSDTKALAITEEPAGGSAAPTTTP
                     KWVGAMS"
     gene            complement(1043..>1379)
                     /locus_tag="VII37_08745"
     CDS             complement(1043..>1379)
                     /locus_tag="VII37_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEY5069865.1"
                     /translation="RHSNSYVVTASVDYIDFRNAVNLGELVILKSQVNRVFNTSMEVG
                     VEVFSEQILTGERKHTTTAYVTFVAIDEYTKQPKTVRPLLLRTPEEKQRFRDAAERRK
                     TRLRLRFGK"
BASE COUNT          315 a          380 c          393 g          291 t
ORIGIN      
        1 tctttggtcg gcgaactgaa tccacggcag aaactcgcta ttgaactggc atacttccaa
       61 ggcttgagtc acagcgaaat agccgcagaa ttaaaggaac cgctcggcac tgtaaaaagc
      121 tggattcgca atggattgat ccggcttaag gaaggattgc gcaccgcgtc gtgagcaatt
      181 gcgatcaatt ccgggagatg tatgaagcct acgcgctggg cgcgctcgat ccgtcagagc
      241 gcgctgcgct ggaagctcat ctcgcgaccg gttgcgcgga atgcctaaag gctgtcgcgg
      301 aagcgcgttg gctggttgct cagttggcgt acctggcgcc cgaggcttcc acgtcggaca
      361 tgctcaaagg ccggttgatg cagaccgtgc ggtcggaggc gaaatcctca gtcccagctt
      421 cgactggagc aaaaacgaga cgggcctcag gcgttcctta ttggttgtcg gcaggaatcg
      481 cggcgctgtt actgttgact ctatacttcg cttgggatgc tcatcggcta agggaggagt
      541 tgcgcgcggc caacgaacaa aatcagcgaa atcaacagct ccttgcgagc acacaaaacg
      601 agcacgcagt gtcccagcaa gagttaacag ccatgcaaga gcaaatgggt aagatgcacc
      661 gcgaaacggc cattttgacc gacccggctt cagtcaagat caccctagcg gcacaaaaaa
      721 ttcaggcccc gcagatggtc gcgatgtggc acgccaagta cggcattgtg cttgccggac
      781 aaaaagttcc gatgccgtcc ggcaatcgcg ttctgcagtt gtggttgata ccaaaagcgc
      841 ctggcggtaa gccgatgccg tcgatgactg tacgtccgga cgccaacggt aacttcgtaa
      901 ttttggttgc gaatccaccc gctgctggca gcgacaccaa ggcgctggct attacggaag
      961 aaccggctgg cggcagcgcg gcgcccacca ccacgccaaa gtgggtggga gccatgagtt
     1021 agtaaagccc gtctgctcga gtttattttc cgaagcgcag cctcaagcgc gtttttcttc
     1081 gctccgctgc gtctcgaaag cgctgctttt cctcgggagt tcttaacagc agcggccgca
     1141 ccgttttagg ctgcttcgta tactcatcaa tggccacgaa cgtcacataa gctgtcgtgg
     1201 tgtgcttgcg ctcaccagtc agaatttgct ccgaaaaaac ttccacgccc acttccatgg
     1261 atgtgttgaa aacccggttt acttgggact tgaggatcac caattcgccc aagttgaccg
     1321 cgttgcgaaa atcgatgtag tccaccgacg ctgtgactac gtagctgttc gaatggcgg
//
LOCUS       DATCBB010000790         1866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_481104, whole genome shotgun sequence.
ACCESSION   DATCBB010000790 DATCBB010000000
VERSION     DATCBB010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481104"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VII37_08750"
     CDS             complement(<1..1555)
                     /locus_tag="VII37_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY5069866.1"
                     /translation="MRKSKVWLGGLSLMILSASLYGPSRAQETHSSSVRAMMPGLNLM
                     PVPANVQMGNGSLKIDAGFTVAINGHTDARLTGAAERFVERLSKQTGLLMAMRPVNGT
                     KATLVVHVDHDSKQVQELGEDESYVLEVAPSGASLTAPTDLGALHGLQTFLQLVSVSP
                     DGFVAPAVIIKDTPRFPWRGLMIDAARHFIPLEVLRRNIDGMEAVKMNVFHWHLSENQ
                     GFRVESRKFPKLHEMGSNGLYYTQDEIRGLIAYARERGIRVVPEFDLPGHSTAWFVGH
                     PELASGPGPFTIERKWGVFDPAFDPTNDKVYKFLDEFIGEMAKLFPDHFFHVGGDEVN
                     GKAWDANPKIQEFIRAHDLKGNAGLQLYFNKKLQGIVSKHGKSMVGWDEVLDPTLPKD
                     IVIQSWRGQASLAEAAKQGYRGILSNGYYLDLGWSAARHYAVDPMGDAAAGLSPEEKQ
                     RILGGESCMWSEYVNAENIDSRIWPRNAAIAERLWSPEEVRDPASMYARMHVLSAQLE
                     WLGLTHENYS"
     gene            1733..>1866
                     /locus_tag="VII37_08755"
     CDS             1733..>1866
                     /locus_tag="VII37_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY5069867.1"
                     /translation="MKTSVAIYPGSFDPVTNGHLDLIERGEKMFDLVIVAVLQNVDKQ
                     P"
BASE COUNT          409 a          644 c          403 g          410 t
ORIGIN      
        1 cggaataatt ctcatgcgtc agccccagcc attccagttg cgcgctaagc acatgcatcc
       61 gcgcatacat ggaagccgga tcccgcactt cctccggcga ccacaaccgc tccgcaatcg
      121 ccgcattacg cggccagatg cgcgaatcga tattctccgc attcacatac tccgaccaca
      181 tgcacgattc cccgcccaga atgcgctgct tctcctccgg cgaaagacca gccgctgcat
      241 cgcccatcgg atcgactgca tagtgccgcg cggcgctcca ccccaagtcc aggtaatatc
      301 cattcgaaag aatcccgcgg tagccttgct ttgccgcttc cgccagtgaa gcctgccccc
      361 gccacgactg gatcacgata tctttcggca gcgtggggtc cagcacttca tcccagccca
      421 ccatgctctt gccgtgcttg ctcacaatgc cctgcagctt tttgttgaaa taaagctgca
      481 atcccgcgtt ccctttcaaa tcatgcgcgc gaataaactc ctgaattttc gggttggcat
      541 cccacgcctt gccatttact tcatccccgc ccacatgaaa aaaatgatcc ggaaaaagtt
      601 tggccatctc cccgataaat tcgtcgagga atttgtaaac cttgtcattg gtcggatcaa
      661 acgccggatc gaaaactccc catttacgtt caatcgtaaa aggccccggc ccgctcgcca
      721 gttccggatg acccacaaac cacgcggtac tgtgccccgg gagatcaaac tccggcacca
      781 cccgaatccc acgctcgcgc gcatacgcaa tcaatccacg aatttcatcc tgcgtgtagt
      841 acaacccgtt cgaacccatc tcatgcagct tcggaaactt cctgctctcg actcggaagc
      901 cctgattttc cgacaggtgc caatgaaata cgttcatctt caccgcttcc atcccatcga
      961 tattcctgcg cagcacctcc agcggaataa aatgccgcgc cgcatcgatc atcaatccgc
     1021 gccagggaaa tcgcggcgtg tccttgataa ttactgcagg cgcaacaaat ccatccggcg
     1081 acacgctcac taattgcaaa aaggtttgca atccatgcag tgcacccaga tccgtcggcg
     1141 ccgtaaggct cgccccactc ggcgctactt cgagcacata ggattcatct tcgccgagtt
     1201 cctgcacctg cttgctgtcg tgatcaacgt gcaccaccag cgttgctttc gttccgttca
     1261 ccggcctcat cgccatcaag agcccggttt gtttcgacag ccgctcgaca aatcgttctg
     1321 ccgcgcccgt cagccgcgcg tccgtgtgac cgttaatcgc cacggtgaat cctgcgtcaa
     1381 tcttgaggct gccgtttccc atctgaacat tcgcgggaac aggcatgaga ttcagccccg
     1441 gcatcatcgc tcgcacgctc gacgagtgcg tttcctgcgc ccgcgacggc ccgtaaagcg
     1501 acgcgctcaa aatcatcaaa cttagtcccc ccaaccaaac cttcgacttc ctcattattg
     1561 tgccgccctc tccgtgaata atctcgccac ttccttagcc atgttcgcgc accgcatact
     1621 ggcacaatca agccgttggc gcagcgtttt tcgctccgcc ccacctttgc ctgtcccgta
     1681 attcacggct gagttagaat aattgcattc tgtgccgctc gacaaaaaca aaatgaagac
     1741 ctccgtcgcc atctatccgg gctcatttga tcccgtcact aacggccacc tcgacctgat
     1801 cgagcgcggc gagaaaatgt tcgacctggt catcgtcgct gtcctgcaaa atgtcgacaa
     1861 gcagcc
//
LOCUS       DATCBB010000791         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_316846, whole genome shotgun sequence.
ACCESSION   DATCBB010000791 DATCBB010000000
VERSION     DATCBB010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316846"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="VII37_08760"
     CDS             <1..1570
                     /locus_tag="VII37_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5069868.1"
                     /translation="IIVGGGHNGLVAAFYLAKAGFKPLVLERRSQVGGAAITEEFHPG
                     FRCSTLAHNAGPLRADIVRDMQLEKHGLKFTTPDVNTVSLTPDGRALILYSDTKKAAQ
                     EIAQWSQKDAASYSDFGAALGKIGKVIGQALLLTPPNIDSPDRGDLWGMLKTGRSIRN
                     LGKKDLYRVLRWGPMAAADLVAEFFDTEPLRATIAARGIFGTFLGPWSAGSALVLMLR
                     AAGDPTPAGSVQFPAGGAGAITQAMAAAARQAGAEIRTDAEIAEIRVKDGIATGVVLA
                     SGEEIAARAVVSNADPKSTLMRLVDPAHLTPDFVQKIKNYRCMGTVAKINLALSALPE
                     FTALKAKGNGNLLQGRIQISPEIDYLERAFDDSKYGNFSRNPYIEITIPSLADPSLAP
                     AGKHVMSIYMQYAPYKLKEGNWDSQREILGEAVVKTISQYAPNFPQTILSGQIITPKD
                     LEETYGLTGGHIFHGELALDQFFTMRPLLDWARYQTPIERLYLCGNGTHPGTGLTGGS
                     GANAAREIVKALKN"
     gene            complement(1700..>2111)
                     /locus_tag="VII37_08765"
     CDS             complement(1700..>2111)
                     /locus_tag="VII37_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069869.1"
                     /translation="RHRHNKVGDVVEVGNALVIGMVGNNQRDFATQFTTLVPIEQILQ
                     AVVVLRNEDGNAWPVRGIGEPPIHLEFAGNRLEALRELRQVKIKIGRIELDSRQKKIR
                     FLISMLIGEKDVSVVAKNEFGNRSDHTFAIGTGN"
BASE COUNT          479 a          579 c          573 g          480 t
ORIGIN      
        1 gattatcgta ggtggcggac acaacggatt ggtcgctgcg ttctatctcg ccaaagccgg
       61 atttaaaccg ctcgtgctcg aacgccgatc ccaggttggc ggcgcggcca tcaccgaaga
      121 atttcatccc ggtttccgtt gctccacgct ggcgcacaat gcgggtcctc tgcgcgccga
      181 catcgtgcgc gatatgcaac ttgagaagca cggactaaag ttcaccactc cagatgtgaa
      241 caccgtgtcg ttaacgcccg acggcagggc gctgatcttg tactcggaca caaaaaaagc
      301 ggcgcaggaa atcgcacagt ggtcacagaa agacgcggca tcctattcgg acttcggcgc
      361 ggctctcggc aagattggca aagtaattgg ccaagcgctc ctgctgacgc cgccaaatat
      421 cgacagcccc gaccgcggtg atctctgggg aatgttgaag acgggtcgtt cgatacgaaa
      481 tctcggcaaa aaagacttgt atcgcgtcct gcgctggggg cctatggccg ctgccgattt
      541 ggtagctgaa ttctttgaca ctgagccttt gcgcgcgact attgcagccc gcggtatttt
      601 tggaacattt ctggggccgt ggtctgccgg cagcgccttg gtgttgatgt tgagagcggc
      661 aggcgatcca actccagcgg gaagcgtgca gttccccgcc ggcggtgcgg gcgccatcac
      721 tcaagctatg gcagccgctg cgagacaggc aggcgcggaa attcgaaccg acgctgagat
      781 tgctgagatt cgcgtaaaag atggcattgc cactggcgtg gtacttgctt caggggaaga
      841 gattgccgcg cgtgctgtcg tctctaatgc tgacccaaag agcacgctga tgcggttggt
      901 cgacccggcg caccttactc cggatttcgt tcagaaaata aaaaactacc ggtgcatggg
      961 aacagtggca aagattaatc tcgccctttc tgctcttcct gaatttacag cgctgaaggc
     1021 taagggaaac ggcaacttac tgcagggccg aatccagatc agtcctgaga tcgactacct
     1081 ggagcgcgct ttcgacgatt caaagtatgg gaatttttca cgcaacccct atattgagat
     1141 tacgattccg tcgcttgcgg atccgtcact ggcgcctgct ggcaagcacg tcatgtcgat
     1201 ctacatgcaa tatgcgcctt ataaactgaa agagggcaac tgggatagcc agcgcgaaat
     1261 actgggcgaa gcggtggtga aaacgatctc gcagtatgcg ccgaattttc cccagaccat
     1321 tctcagcgga cagatcatta cacccaaaga tctcgaggaa acttacggcc ttacgggagg
     1381 acatatcttc cacggcgaac ttgcccttga tcaattcttc actatgcgtc cactgctcga
     1441 ctgggctcgc tatcagactc ccatcgaacg cctgtattta tgcggcaacg gcacccaccc
     1501 gggaacagga ctcaccggcg gttcgggggc taatgccgcg cgcgagatcg tgaaggcgct
     1561 gaagaattag gcagatttgg gtagatatga tttccccgtg ctgatggagg aagtttcttt
     1621 tgccgcaggg gaaaacagag aaacgcaagg aaaaagcgac ggcaccaggg ctttgtcaat
     1681 gcataattcc gccatccttc taatttcctg tcccgatcgc aaaggtgtgg tcgcttcgat
     1741 tgccgaattc atttttcgcc acaacggaaa catccttttc gccgatgagc atggagatga
     1801 ggaatcgaat ctttttctga cgcgagtcga gttcgatccg gccgattttg attttgactt
     1861 ggcgcagttc cctgagcgct tccagccgat tgccagcgaa ttccaaatgg attggcggct
     1921 cgccgattcc tcttaccggc cacgcattgc catcttcgtt tctaagtacg accactgctt
     1981 gcaagatctg ctctatcggc accagagtgg tgaactgcgt tgcgaaatcc cgttgattat
     2041 ttccaaccat cccgataacc agcgcattgc cgacttctac cacatcccct actttattgt
     2101 gccggtgacg a
//
LOCUS       DATCBB010000792         1510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_381522, whole genome shotgun sequence.
ACCESSION   DATCBB010000792 DATCBB010000000
VERSION     DATCBB010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381522"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VII37_08770"
     CDS             <1..533
                     /locus_tag="VII37_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5069870.1"
                     /translation="MDWLMNVNFWGVVHATRAFLPHLAQQREAHIVNVSSIFGIIAPP
                     GQTAYAAAKFAVRGFSESLRHELETAGSPIKLSLVHPGGVVTNIARNACSGVALADSS
                     RRAEALERFDSVARTTPKAAAQRIIEGVEHNHPRILIGFDAQFADNLQRLQPETYWKQ
                     LANIFNERKAPETGKS"
     gene            717..1277
                     /locus_tag="VII37_08775"
     CDS             717..1277
                     /locus_tag="VII37_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069871.1"
                     /translation="MQPAILFAGTQQSHPAHPHQRVYLLRGFLNVFSLGMDQLANELH
                     QRGIEAVVANHTLSGFYGNEAIRDCRAGLISSIAIVGHSLGASAGVDMAEQLAQAGIT
                     VGLVVTVDPITPTAAPGNVKRVENFYISDGVGKIVQRGRDFHGSLQNIDLKRDAGIGH
                     VSIANSEHVHQQVLRFVAAAATSRCK"
BASE COUNT          325 a          405 c          440 g          340 t
ORIGIN      
        1 agatggactg gctgatgaac gtgaatttct ggggcgtcgt gcatgccacg cgcgcgttcc
       61 tgcctcatct ggcgcagcag cgcgaggccc acatcgtaaa tgtatcgagt atcttcggca
      121 tcatcgcgcc gcccggccag actgcctatg cggcggcaaa attcgcggtg cgtgggtttt
      181 ccgaaagctt gcggcacgag cttgaaacgg caggaagccc aatcaagctt tcgctggtcc
      241 accccggcgg agtggtgacc aacattgcgc gtaacgcctg ttctggcgtc gcgcttgcgg
      301 acagttcgcg tcgagctgaa gcgctcgagc gtttcgatag cgtcgcgcga accaccccga
      361 aagccgctgc gcagcgcatc attgagggcg ttgagcacaa tcacccgcgc atcctgattg
      421 ggtttgacgc gcagtttgcg gacaatctgc agcggctcca acctgaaacg tattggaagc
      481 aactggcgaa tatattcaat gagcgaaagg caccagaaac cgggaaaagc tagatcgccg
      541 ccaacgaggt tttgatcgga gcggccacag atcccggtgt cgccagattc gattccggaa
      601 gccgttgact gagaaaacaa tggcatttat ttatctgcgt ctattcctca aaataaagta
      661 ggaccgggat gcgctgctcg aaaccttacg ccgcatgtgc ggtatttttc tttctgttgc
      721 agccagcgat tctgttcgca ggaactcagc aatcccaccc tgctcatcca catcaacgcg
      781 tgtatctgtt gcggggcttt ttgaacgtct tttcactggg aatggatcaa ctagccaatg
      841 aactccacca gcgaggaatc gaggccgtcg tcgcgaatca tacgctttct ggcttctacg
      901 gcaatgaagc gatccgtgat tgcagagcgg ggttgataag ttccattgcc attgttggcc
      961 attcactggg agctagcgcg ggggtcgata tggcggaaca attagctcag gcaggcataa
     1021 cggtcgggct ggtcgtcacg gtggatccaa ttacgccgac cgctgctcct ggcaacgtca
     1081 agagagtgga aaacttctat atttccgacg gggtcggtaa gatcgtccaa cgtggccggg
     1141 attttcacgg atctttgcag aatatcgatc tgaagcgcga cgctggtatt ggccacgtct
     1201 cgatagcaaa ttcggagcac gtccatcagc aggtgttgag gtttgtagcg gcggcggcaa
     1261 ccagccggtg caaataaaca gccagccagc cgccggcggc attcttagtg ccgtattatt
     1321 gctgttaccg acctgttttg cggagtgtgc gcgtattgcg ggactttcgg caccttattc
     1381 ggggcggttc tatcgcgagg agcagacgat ggtgaaccag tttggcggcc gctatcgtaa
     1441 ctacgccaaa cctgccgcac gtatcgtccc atggcttctt tagctgaaag ggagcttgac
     1501 cgtcgacagg
//
LOCUS       DATCBB010000793         1338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_57929, whole genome shotgun sequence.
ACCESSION   DATCBB010000793 DATCBB010000000
VERSION     DATCBB010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57929"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1122
                     /locus_tag="VII37_08780"
     CDS             118..1122
                     /locus_tag="VII37_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5069872.1"
                     /translation="MNPSRGLALVGIIFACVILHELGHAIVGRQEGLPAKAIILLPIG
                     GITLLDETQQAPDPQSQTWKRDIRIALAGPAVNLAIAAVAAGIFYLVSPEINLWAKPY
                     VHSSNLARSFVWSNLWLAAFNLLPAYPMDGGRVLRAVFTRRMDSVSATKRAVTIGQGF
                     ATLFMLTGMLWNIWMTLIGFFLFLAAQLEERSAVFQSVLETVRLEDIMLTDFATLSPA
                     DTLEDALEKAVHSLQDDFPVIRGSDMVGVISRQKILQVLRVEGNGYVQAVMNRIFEVA
                     QKKESLASAFRKLTARNLSIIPIVEDQRLVGMVTLQNLMHSMALLAESRKLRKAALNS
                     "
BASE COUNT          268 a          355 c          377 g          338 t
ORIGIN      
        1 atgagaaatt ggtctatttc ggcggggcga attttcgggg tggagctccg aattcatctt
       61 agcttcgttt tcctacttgt ttttgtgttg ttaacggact cggcgtcacc caccacgatg
      121 aacccctctc gaggacttgc actcgtgggc atcattttcg cgtgcgtgat tttgcacgaa
      181 ttagggcacg caatcgtggg cagacaagaa ggactccccg ccaaggccat cattcttttg
      241 ccgatcggcg gcattaccct gctcgacgag actcagcaag cgcctgatcc gcaatcgcag
      301 acgtggaaaa gggatattcg tatcgcactc gcgggaccag cggttaatct ggcgattgcc
      361 gcggttgctg caggcatttt ttatctggta tcgcctgaaa ttaatctgtg ggcaaaacct
      421 tatgtgcact cgagcaatct tgcgcgcagc tttgtttggt ccaatctgtg gctggcggca
      481 ttcaatttgt tgcctgccta tcccatggac ggcggccgcg tattgcgggc agtattcact
      541 cgccgcatgg attcggtcag tgccacgaag cgcgccgtaa ccattggtca gggttttgcc
      601 acgctgttca tgctcaccgg gatgctctgg aacatctgga tgaccctgat tggcttcttc
      661 ctgtttttgg ctgcgcaact ggaagagcgg tcggcggtct tccaatcagt tcttgagacg
      721 gtgcggctgg aagatatcat gctcaccgac ttcgcaacgc tctcgcccgc ggacacgctt
      781 gaagatgccc tcgaaaaggc ggttcattcc cttcaggatg atttccccgt gatccgcggc
      841 agcgacatgg tcggagtgat ttcccggcag aagatcctgc aggttctgcg agtggaaggg
      901 aacggttatg tacaggcggt gatgaatcgc attttcgagg ttgcgcaaaa gaaggaatcg
      961 ctggcctcgg cgttccgaaa gttaaccgcg cgcaacctta gcatcattcc tatcgtggaa
     1021 gatcagcggc tggtaggaat ggttaccctg cagaacctga tgcactcgat ggcgttattg
     1081 gcggaaagcc gcaaattgcg caaagcagcg ctgaattcct agcgattgcg gcacgcgctg
     1141 gagcttgcaa aggcaatccc aagcgggagg gcccgacttt tcagtcgggc cgatcattga
     1201 cttgcgttgg tttacggccc gactggaagt cgggccctac cctttttgcg ccgttcaatt
     1261 tgaatttgtc cgaagcctgg ccagatcctt cagaatttaa atactatgcc aatcgccgct
     1321 tcggctacgt gtcccctg
//
LOCUS       DATCBB010000794         1045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_57949, whole genome shotgun sequence.
ACCESSION   DATCBB010000794 DATCBB010000000
VERSION     DATCBB010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57949"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1045
                     /locus_tag="VII37_08785"
     CDS             <1..>1045
                     /locus_tag="VII37_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069873.1"
                     /translation="IGYPVMVKAAMGGGGRGMRVVHDAKELDGLLEEAQGEARSAFGD
                     GSVFLGKYLAHARHLEVQILGDEHGDLLHLYERDCSVQRRHQKVVEVAPAANLPPGVR
                     SELCDAAVKLASKAGYRNAGTVEFLYDVDAGKFYFIEVNPRIQVEHTVTEMVTGIDLV
                     RSQILVAQGHHLHEAPLSLPAQEKIPLYGAALQCRVTTEDPANNFSPDYGKISTYRSP
                     AGFGIRLDGGTAYAGAILAAYYDSLLVKVTAWGSNLPEACQRMDRALREFRIRGVKTN
                     IPFVENVVNHPKFRAGEVTTSFLDDSPELFRFRTRGDRATKLLSYLGDVILNGNPEVK
                     GKKGPEIRETPVVP"
BASE COUNT          220 a          245 c          332 g          248 t
ORIGIN      
        1 attggttacc cggtaatggt gaaagccgcg atgggcggcg gcggacgcgg gatgcgcgtt
       61 gttcatgacg cgaaggaatt ggatggctta ttggaagagg cccagggaga ggcgcgatcg
      121 gcatttggcg atggctcggt attcctgggg aaatatcttg cccacgctcg tcatctcgag
      181 gtgcaaattc ttggggatga gcacggcgat ctgctgcatc tatacgagcg cgattgttcg
      241 gttcagcgcc gccatcagaa agtagtagaa gttgcgccgg cggcaaattt gccgcctgga
      301 gtgcgcagcg aactatgcga cgccgcggtg aaattggcga gcaaagctgg ttatcggaac
      361 gccggaacgg ttgagtttct ttacgacgta gacgctggga aattttattt catcgaagta
      421 aatccgcgca ttcaggtgga acacaccgtt acagagatgg tgacgggcat cgacctggtg
      481 cgctcgcaga ttcttgtggc acaggggcat cacttgcatg aggcgccgct gtcgctgccg
      541 gctcaggaaa aaattccgtt gtatggtgct gcgctgcaat gccgagtcac taccgaagat
      601 cccgcgaata atttttctcc agactacggg aagatttcga cctaccgttc gccggctggt
      661 tttggtattc ggctggatgg cggaacggct tatgcgggcg cgattctcgc ggcttattac
      721 gattctttgt tggtcaaagt gactgcttgg ggttcgaacc tgccggaggc gtgccagcgg
      781 atggaccgcg ctttgcggga atttcggatc cgcggcgtaa agacgaacat tccgttcgtg
      841 gagaacgttg ttaatcatcc caaattccgc gccggggaag ttaccacatc atttcttgac
      901 gattcgccgg aactttttcg gtttcgcacg aggggcgatc gcgccacgaa acttttgtcg
      961 tatcttggcg atgttatttt gaatgggaac ccagaggtga aagggaagaa aggtccggag
     1021 atccgagaga ctccggtagt tccgg
//
LOCUS       DATCBB010000795         1372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_123227, whole genome shotgun sequence.
ACCESSION   DATCBB010000795 DATCBB010000000
VERSION     DATCBB010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123227"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..539
                     /locus_tag="VII37_08790"
     CDS             57..539
                     /locus_tag="VII37_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069874.1"
                     /translation="MGKFFGLTGTLVVNTLFMAAGVLLALLYTAHKFEKMDLWVLVAL
                     YFIVLQFLIICALALFFSSFSTPLMSAVFTFALFIIGSLAEDLRGFARIAHGPGGLLA
                     TGIAYLVPNFSALNVISQVAHGDAVAGRLILYNSLYALLYSTMVISGAVLIFQRRNLK
                     "
     gene            536..>1372
                     /locus_tag="VII37_08795"
     CDS             536..>1372
                     /locus_tag="VII37_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069875.1"
                     /translation="MSVTRRINILTTAFFLFSMSGAVAMLYAIDHMERRAVAQQSLYI
                     NSPQVLRRLSLGYTGLLADIYWTRAVQYFGEQHHKGSGDFRLLAPLLEVTTELDPRLL
                     PAYQFGANFLAPKPPAGAGLPGSALALMKYGIAHNPDQWRLYYNLGFLYYTELRDYDL
                     AANAFMQGAKLPNTNPFMPILAAQMAQHAGEFDTARMLWFTTYQSTKDSNIRENAVEH
                     LQALRVDEDVTQLEQLVARYREKTDRFPATMGELERAGFIRGAPVDPKGHPYRLMADG
                     RIE"
BASE COUNT          280 a          360 c          385 g          347 t
ORIGIN      
        1 acaagcgtac gctttacacg gtgctgtcca ggccggtgcg acggtgggag ttcgttgtgg
       61 gcaagttttt cgggctcaca gggacgctgg tggttaacac tcttttcatg gcggccggtg
      121 tgctgttggc gctcctctac accgcgcata aatttgaaaa gatggatctg tgggtcctgg
      181 tcgcattgta ttttatcgtg ttgcaattcc tgatcatttg cgcattggca ttgttcttct
      241 catctttttc aacacctctc atgtcggcgg tgttcacctt tgcgctcttc attatcggaa
      301 gtctagcgga ggacctgcgg ggatttgccc gaattgcaca cgggcccggc ggcctgctgg
      361 cgactggcat cgcatatctg gtgccgaatt tttctgcttt aaacgtgatc agccaagtgg
      421 cacacggcga tgctgtggct ggacgcctga ttctgtacaa ctctctctat gccctgttgt
      481 actccaccat ggtgatttcg ggagcggtac ttatattcca gcgccggaat ctgaaatgag
      541 cgtgacgcga cggataaata ttcttactac tgcctttttt ttattttcga tgagcggcgc
      601 cgtggcgatg ctgtatgcca tcgaccatat ggaacgcagg gcggtagcgc agcagagcct
      661 ttatatcaat tctccccagg tgctgcggcg tttgagtctg ggatatacgg gcttactcgc
      721 ggatatttac tggacgcgtg cggtgcagta ttttggagaa caacaccaca agggctcagg
      781 cgatttccgc ctgctggcac cgctgctgga agtgaccacg gaactcgatc cgcgacttct
      841 gcctgcttat cagtttggcg ccaacttcct tgctcctaaa ccgccagccg gcgcgggatt
      901 gcctggctcc gcactcgccc tgatgaaata tggaatcgcg cacaatcccg atcagtggcg
      961 gctctactat aaccttggat ttttgtacta cacggaatta agggattacg acctagcggc
     1021 gaacgctttt atgcagggtg caaagcttcc gaacaccaat ccatttatgc ccatcctggc
     1081 ggcccagatg gcgcagcatg cgggcgaatt cgataccgcg cgaatgttgt ggttcaccac
     1141 ttaccagagc accaaagatt cgaatattcg ggagaatgcg gttgaacatc tgcaagccct
     1201 gcgagtggat gaggacgtga cccaactgga acaactggtg gcaaggtatc gcgaaaagac
     1261 tgaccgtttt ccggctacca tgggcgaact ggagcgggcg ggatttatac gcggcgctcc
     1321 cgtcgatccc aaggggcacc cctacaggct aatggcggac ggacggattg aa
//
LOCUS       DATCBB010000796         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_481248, whole genome shotgun sequence.
ACCESSION   DATCBB010000796 DATCBB010000000
VERSION     DATCBB010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481248"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VII37_08800"
     CDS             <1..824
                     /locus_tag="VII37_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5069876.1"
                     /translation="EVAAGYTTFATIGTRAEAEFLKRVVGADGKTLEEVEPQTRAALD
                     PRVAYLVTSVLKDVLNKGTGASVRAKGFTLPAAGKTGTSRDGWFAGFTSNLVCVIWVG
                     FDDNRDLGLTGGVAVAPIWADFMLRATALPAYHDVKDFPMPEGVQAVMIDPESQELAT
                     PSCPTTESEVYVTGSAPTQLCELHGGRAAITNGSWLSHIFGGGQPKPPQIGADGKPID
                     PGQVQAGAAPEPPADANGANGQKKKNALQKIFGIFGGKKKDPDKAKPPPEKGDSP"
     gene            821..1654
                     /locus_tag="VII37_08805"
     CDS             821..1654
                     /locus_tag="VII37_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069877.1"
                     /translation="MIRFHAAVPASFLLLSFTFSIPSAGQTFGRKAMVSDAPAVQQER
                     VDSSAIVVRPALTTEQLADLYMVRKDYREAAQTYKRLADENPRNAVYLNKLGIALHQQ
                     EALTLALKYYERATKVDPNYADAQNNIGTVWYQRKKYGKAIKSYQKAIKLRTDMAVLY
                     SNLAYAYFGDKKYDQSLAAFHQALTIDPQFFEHNGSRNGSVLQDRSVTDRGRFYYLLA
                     KSFAQAGNIDRCIVYLRKAKDEGFKELNNVKTDPDFASMLKNPEIQEILAPKPVESAE
                     R"
     gene            1769..>2255
                     /locus_tag="VII37_08810"
     CDS             1769..>2255
                     /locus_tag="VII37_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069878.1"
                     /translation="MPRSYIHEILIVFVASLFFPTWNFCSPQSTQASPNASSRSATGT
                     ATGNAAGAGAGTSSRTTPGTTPGTSSDTTTRKGGSSTGIYLVFPFQNSGPASRLDWLS
                     EGLEELTIQGLSSAGEQVYSHSWRLGELERYGIPTTAKLSRATMLHVAEDLDADFVVF
                     GS"
BASE COUNT          513 a          549 c          734 g          459 t
ORIGIN      
        1 tggaagtggc ggcgggatat accacgttcg cgacgatcgg gacgcgcgcg gaggcggaat
       61 ttttgaagcg ggtggtggga gcggacggga agacgctgga agaggtggag ccacaaacgc
      121 gcgcggcatt ggatccgcga gtagcgtacc tggtgaccag cgtattgaag gatgtgttga
      181 acaagggaac gggagcgagc gtgcgcgcga aaggttttac gctgcctgca gcgggaaaaa
      241 cgggaacgtc gcgggacggg tggttcgcgg gatttacgtc gaacctggtg tgcgtgatct
      301 gggtgggatt cgacgataat cgcgacctgg gactgacggg cggcgtggct gtggcgccga
      361 tctgggcgga cttcatgtta cgggcgacgg cgttgcctgc ctatcacgac gtgaaagatt
      421 tcccgatgcc cgagggagtg caggcggtaa tgatcgatcc ggagtcgcag gagttggcga
      481 cgccgagttg cccgacgacg gaatcggaag tttacgtgac gggttcggcg ccgacgcagc
      541 tgtgcgaatt gcacggcggg cgcgcggcga taacgaacgg atcgtggctt tcgcatattt
      601 tcgggggcgg gcaaccgaag ccgccgcaaa tcggcgcaga cgggaaaccg atcgatcccg
      661 ggcaggtcca ggcgggggct gcgccggagc ctccagcgga tgccaacggg gctaacgggc
      721 aaaagaaaaa gaacgcttta cagaagattt ttggtatctt tgggggcaag aagaaggatc
      781 ccgacaaggc caagcctccg ccggagaagg gagactcccc atgatcaggt ttcatgcggc
      841 agttccagca agttttctcc ttctaagttt tactttcagc attccttccg caggacaaac
      901 tttcggacgc aaagcaatgg tgtcggatgc acccgcggtg cagcaagagc gggtggattc
      961 ctccgcaata gtggtgcgcc cggcgcttac gacggagcaa ctcgccgatc tgtacatggt
     1021 gcgcaaggac tatcgcgaag cggcgcaaac gtacaagcgg ctggcggacg agaacccgcg
     1081 caacgcggtg tatttgaata agctgggtat cgcgctgcac cagcaagagg cgctgacgct
     1141 ggcgctgaag tattacgagc gggccaccaa ggtcgatccg aattacgcgg acgcgcaaaa
     1201 caatatcggg acggtgtggt accagcgcaa gaaatatggg aaagccatca agtcgtacca
     1261 aaaagctata aagctacgca ccgacatggc agtgctgtac agcaatttgg cttacgccta
     1321 ttttggggat aagaaatacg accaatcgct ggcggcgttt caccaggcgc tgaccatcga
     1381 cccgcaattt tttgagcata acggttcgag gaatgggtcg gtgttgcaag accggtcggt
     1441 gacggatcgc ggacgttttt attacctgct ggcgaaatcg tttgcgcagg ccgggaatat
     1501 tgatcggtgc atcgtttatt tgcgcaaggc caaggatgaa ggtttcaaag agttgaacaa
     1561 cgtaaagacg gatccagatt ttgcgagcat gctgaagaat ccggagattc aggaaattct
     1621 ggcaccgaag ccggtggaga gcgcggaacg gtagtcgccg cgttttgtga gcgggatttc
     1681 gggaaatacg ttgcggtgtc actgagtgtt agtgtggaac atcgcgagca attagagtgg
     1741 ttcgcctctg atacaatgct ggaacatggt gccgcgtagt tacattcatg aaattctaat
     1801 tgtttttgtt gccagcttat tttttcccac ctggaatttt tgttcaccac aaagtacgca
     1861 agcttcgcct aacgcttctt cgcggagcgc cacaggcacg gctacaggaa atgcggcagg
     1921 cgcgggggcg ggcacatcat caaggacaac gcccggcaca acgcccggca catcatcgga
     1981 cactacgact cgcaagggcg gttcgtcgac cggtatttat ctagtttttc catttcaaaa
     2041 ttccgggcct gcatctcgtc ttgactggct ttccgaagga ttggaagaac tgaccatcca
     2101 gggactgtcg tctgccggcg agcaggtcta ttcgcactca tggcggttgg gagagttgga
     2161 gcgctacggc attcccacca cagcgaaatt gagccgtgcg acgatgttgc atgtggcgga
     2221 agatctggac gccgacttcg tggtgtttgg cagct
//
LOCUS       DATCBB010000797         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_25586, whole genome shotgun sequence.
ACCESSION   DATCBB010000797 DATCBB010000000
VERSION     DATCBB010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25586"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VII37_08815"
     CDS             <1..1328
                     /locus_tag="VII37_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069879.1"
                     /translation="GPHLYPAPAARERFLSKLAEYGVLRNYEETLRRKDGTLLHTLQN
                     ITAVRDASGRIAQIRGLMLDVTEQRTFQSQLQRERDFNQKILNTTQSMILVLDTAGLI
                     SYANRRCYEMGYQESDLIGHRLVDWIEPGHRQDFEDALDTTAHGQQVENIELRVRRSD
                     NSMGHFSISLSPMRDELKTVNSVVVVMTDITDATLLQAKLAHSEKMATIGRLVSGVAH
                     EVNNPLAAILGFTDLLLENPEVPASAREDLQIILQETQRTKDIVQDLLSFARQRPVQR
                     ELVSVNNVLRQTIKLRSYDFASHGVEVLEEFDEALPPALGDAQQLQQVFLNILNNAYD
                     AVQESTERGRITIRTCHHNHGIEVNIIDNGTGIVDPQRIFDPFYTTKHAGKGTGLGLS
                     ICYGIVRAHGGEIQCWNNEGVSGSTFMVRIPLADTESAAATVSAKEAGR"
     gene            1325..1723
                     /locus_tag="VII37_08820"
     CDS             1325..1723
                     /locus_tag="VII37_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5069880.1"
                     /translation="MSLQSTMWTGGPILLIEDEPSVIAFLRAALERRGYSVVNAGSGS
                     EGLKRLSDGRFAGVISDIRMPGKVNGAEVHAWIQKNRPELRTRIILISGDTANSETQT
                     LLAKSGTPCIEKPFRVQQLMSVVEKTFGKP"
     gene            1720..>2547
                     /locus_tag="VII37_08825"
     CDS             1720..>2547
                     /locus_tag="VII37_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5069881.1"
                     /translation="MSDDLKTRLLVVDDEQSIRRLCMTIGTALGFSCTEAESAEAALP
                     RLEADTPDLVLTDLKLPTQTGVALLKQTKAILPRTEVAIMTGHGSIESAVDAMKLGAY
                     DYIEKPFRVEKMRMLLQRMAEKVRLVTENQFLRERVNTEENLDGIIGTSANIQDVLRM
                     ISRLKDTRTPVLVSGESGTGKELVARAIHFRGALAQTAFVAVDCGSLVPTLMESELFG
                     YEKGAFTGAMKTKAGLFQAANGGTIFLDEIGELPLEMQAKLLRVLQEKEVRPVGSNEK
                     "
BASE COUNT          590 a          654 c          744 g          559 t
ORIGIN      
        1 taggtcctca tttgtatccg gctcccgccg cgcgcgagcg ttttctttca aagctggctg
       61 aatatggcgt cttgcgaaat tacgaagaaa ccttgcggcg caaagacggc acgcttcttc
      121 acacgctgca aaacattact gccgtccgtg atgccagcgg tcgcattgcg cagattcgtg
      181 gcttgatgct ggacgttacc gagcagagaa catttcagtc acagctgcag cgcgagcgcg
      241 attttaacca gaagattttg aatactacgc aaagcatgat cctggtgctg gatacggccg
      301 ggctgatcag ctatgcgaat cgccgctgtt atgaaatggg ttaccaggaa agcgatctga
      361 ttgggcaccg gttggtggac tggattgagc cgggccaccg gcaggatttt gaggacgcgc
      421 tcgacactac tgcgcacggg caacaagttg aaaacatcga gctccgtgtg cgccgcagcg
      481 ataattcgat gggacatttt tccatcagcc tgagccccat gcgcgatgag ctaaaaacgg
      541 ttaacagcgt tgtggtggtg atgaccgaca taaccgatgc tacgttgctg caggccaagt
      601 tggcccacag cgagaaaatg gctaccatcg gccgcttggt ttctggcgta gcccacgaag
      661 tgaacaaccc gctggcggca atcctgggat ttacggattt gctgctcgag aatcccgaag
      721 tgccggcctc ggctcgcgaa gatttgcaaa tcattttgca ggagacgcaa cgcaccaagg
      781 atattgttca ggacctgttg agttttgcgc gccagcgccc ggtgcagcgc gagcttgtca
      841 gtgtgaacaa tgtactgcgc cagaccatca agctgcgcag ctacgatttc gctagtcatg
      901 gcgtggaagt gttggaggag tttgatgaag cgctgccgcc cgcgctggga gacgctcagc
      961 aattgcagca agtttttctg aatattttga ataacgccta cgacgccgtc caggaatcca
     1021 ctgagcgcgg acggatcacc attcgaacct gtcatcataa tcacggcatt gaagtaaaca
     1081 tcatcgacaa tggcactggc atcgtcgacc ctcaacgcat tttcgatccg ttctacacca
     1141 ccaaacatgc cggcaaggga acaggcctag gcttgagtat ttgttacggc atcgtgcgcg
     1201 ctcacggcgg cgagattcag tgctggaaca acgagggcgt gtccggtagc acatttatgg
     1261 tgcgcattcc gttggcggac acggaatcgg ccgctgctac ggtctccgcg aaagaggccg
     1321 gtcgatgagc ttgcaatcca cgatgtggac tggtgggccg attctgttaa ttgaggatga
     1381 gccctcggta attgcatttc tgcgcgcggc gctggagcgt cgaggatatt cggtggtcaa
     1441 cgccgggtct ggcagcgagg gcttgaaacg cctgtctgat ggcaggtttg caggcgtcat
     1501 ttctgacatt cgcatgcctg ggaaagtaaa tggagccgaa gtgcacgcct ggattcagaa
     1561 aaaccggccg gagctccgca cgcgcatcat tttaatcagc ggagacactg ccaacagcga
     1621 aacacaaact ctgctggcta agagcggtac tccgtgtatc gaaaagcctt tccgtgttca
     1681 gcaattaatg tctgtagtgg agaaaacctt cgggaaacca tgagcgacga tttaaaaaca
     1741 cgtttgttag ttgtagatga cgagcaaagt attcgccgac tgtgcatgac cattggcacg
     1801 gcgctgggtt tttcctgtac cgaagcggaa agcgcggaag cggcgcttcc ccgccttgaa
     1861 gccgacaccc ccgacctggt gcttactgac ctaaagttac ccacgcaaac cggagtggcg
     1921 ttgcttaaac agaccaaagc gatacttccc cgcactgaag ttgccatcat gaccggccac
     1981 ggttcgatcg aatccgccgt ggatgccatg aagctgggcg cttatgacta catcgagaag
     2041 ccttttcgtg tcgaaaagat gcgcatgttg ctgcagcgca tggccgagaa ggttcgcctg
     2101 gtcactgaaa atcagtttct tcgcgaacgc gtcaacaccg aagagaacct cgatggcatc
     2161 atcggcacgt ccgccaacat tcaagatgtg ttacgcatga tttcgcggct gaaggacacg
     2221 cgcacgccgg tcttggtgtc tggcgagagt ggcaccggaa aggaattggt ggcgcgcgcc
     2281 attcattttc gcggggcgct cgcccaaacg gcttttgtgg ccgtggattg tggttcgcta
     2341 gtgcccactt tgatggaaag cgaacttttt ggttacgaaa aaggtgcgtt tacgggcgcg
     2401 atgaaaacca aggcgggcct gttccaggcc gccaatggag gtacgatttt tctggatgaa
     2461 attggcgaac tgccgttgga gatgcaggcc aagctgctgc gcgttttaca ggagaaagaa
     2521 gtgcggccgg tgggcagcaa tgaaaag
//
LOCUS       DATCBB010000798         5178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_415138, whole genome shotgun sequence.
ACCESSION   DATCBB010000798 DATCBB010000000
VERSION     DATCBB010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415138"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VII37_08830"
     CDS             <1..315
                     /locus_tag="VII37_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069882.1"
                     /translation="SLQKYHHYYGEDFKLECPTGSNNQTNLWQVAAEISRRLTHIFLR
                     DDNGRRPIYGDTEVFQSDPYWRDLVLFYEFFNGDNGAGLGANHQTGWTGLVAKMIQQS
                     GE"
     gene            complement(322..747)
                     /locus_tag="VII37_08835"
     CDS             complement(322..747)
                     /locus_tag="VII37_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069883.1"
                     /translation="MNPKPIVTVGVAVRRETFTRIRAANGQAELADVPQEQDAEEFEL
                     HFADGVELDVLTSREPSGQGAIARFLEKFGEGVQQVEFRCLNVDRAMAILRERFRLGP
                     IYPEARSGANGSKVNFVLVAVPSSGVEKAKVLIELYELP"
     gene            complement(912..1868)
                     /locus_tag="VII37_08840"
     CDS             complement(912..1868)
                     /locus_tag="VII37_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY5069884.1"
                     /translation="MGTLSRWIFFNIFVLVAVALDLGVFHRRPHRIRLRDAAIWSAGW
                     VALSIFFGVGVFWFLGRQPALEFFTGYLIEKALSIDNLFLFLVIFQAFSVDERVQHRL
                     LAWGVLGALVMRGIMIAMGAALVSRFAWIMYVFGAFLVYAGVHMLLIRTRETRPEQNK
                     IFQLASRHLRVTRQYQGERFFVRSNKQLFATPLFLVLLVVEITDITLAVDSIPAIFGI
                     THDAFIVYTSNVFAILGLRAIYFLLAGALQRLRYLTVGLAGVLVFIGGKMIVEPWVHV
                     PVHISLGVVAGILLVALVVSLMSPAAPVEQTRSKIRRGSDKT"
     gene            complement(1886..2737)
                     /locus_tag="VII37_08845"
     CDS             complement(1886..2737)
                     /locus_tag="VII37_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069885.1"
                     /translation="MAVLAVLLGSLLISTSACAQTATGTGGLQLTAKITPTAARPEPV
                     RQFTFYVLSKSYAAVAKEVEADDAMPSRDKFIDDLKVSPELKEWLKAHEILDLTMPGL
                     DKALKADNVLHVPEFLLAYQRSNSGGVTNGIPKPKYKDADKTEHPEKYEKQKEQYYAA
                     LRKFIGAHPETMSGMELEMDAANPQRKWAKLESEHKKNVQHNAPNVAQTKYLVAKADT
                     DLDGRAGVSGLPPGSYWVSSLNLDANAGDTRVRWDVPVAIQPGQTTRVELNNLNATDA
                     RAAASAP"
     gene            complement(2901..4124)
                     /locus_tag="VII37_08850"
     CDS             complement(2901..4124)
                     /locus_tag="VII37_08850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY5069886.1"
                     /translation="MINLLPPQPTKPSDPTASTPGGPPAVPLPRFGTPSRFVTDGFWG
                     NLKQFLTERPVKLSGDVHSPLMPENYGDGFKENLKTVLKGGPVPKGASNSRLEVAWGA
                     GFGGFGDRLKELFFPVKQAPAQWTSKPVKVKDIWSKDENFGWTQIISIGLHVGIITLL
                     VVPLFTHILPSSTQAKNKFDSTPLDISPYIAKLPAGTKKAGGGGGGGDRSITPPTKGR
                     APKFAYTQFTPPEATLKNPAPKLPMDPTLLGPPELKIANPPLTNMGDPMAGAVTMSGG
                     PGGGGGIGVGTSGGIGSGTGAGLGPGETAGTGGGAFRAGVNGVGSPQCIYCPQPEYSD
                     EARKAKYQGTVLLDVTVTADGRVTNPVVIKGPGLGLEEKALAQVRNWKMRAALGPGGK
                     AVACRVQIEVTFHLY"
     gene            complement(4266..4424)
                     /locus_tag="VII37_08855"
     CDS             complement(4266..4424)
                     /locus_tag="VII37_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069887.1"
                     /translation="MESPLLSLAVTDGAETLFPEPVASAINAYFPRIYTASLLLEVVY
                     DGKHRKAG"
     gene            4435..>5178
                     /locus_tag="VII37_08860"
     CDS             4435..>5178
                     /locus_tag="VII37_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5069888.1"
                     /translation="MAEPLELKKTLNLPKTDFPMKASLPQNEPKQLAAWEEAHLYEKI
                     LETRKGKPLFVLHDGPPYPTGTIHLGTGLNKILKDMIVKSESMAGHYAPYVPGWDCHG
                     LPIETQVEKELGGKGKVSAPEFRKLCRQFANRYVEQHKRDFKRLGVFGRWNDPYLTMN
                     FDYEATIAGAFLEFMEKGYVYRGRKPVYWCLHDRTALAEAEVEYEDHTSPSVWVKFAI
                     ADGQKPGNEVSAVIWTTTPWTLPHNRALAF"
BASE COUNT         1238 a         1614 c         1199 g         1127 t
ORIGIN      
        1 tccctgcaga aataccatca ctactatggc gaagacttca aactcgagtg tccgaccggt
       61 tccaacaatc aaaccaatct ctggcaagtc gccgcggaaa tatcacgccg gctcacccac
      121 atttttctcc gcgacgataa cggccgccgt cccatttacg gtgacaccga ggtattccaa
      181 agcgatccgt actggcgcga cctcgttctc ttttatgaat ttttcaacgg agacaacggc
      241 gccggcctcg gcgccaatca tcaaaccgga tggaccggcc tggtagccaa aatgattcaa
      301 caaagtggcg agtgatcccg cttacggtaa ttcgtaaagt tcgattagca ctttggcctt
      361 ctcaaccccg gagctaggaa ctgctaccag aacaaaattc accttcgaac cgtttgcccc
      421 ggaccgcgcc tctggataaa tgggcccaag cctaaaccgt tcgcgtaata tagccatcgc
      481 gcggtcgaca ttcagacatc gaaactccac ctgctgaacg ccctcgccga acttctccag
      541 aaatcgcgca atcgccccct gaccgcttgg ctcccggctc gtcagaacat ccaattcaac
      601 gccatccgca aaatgcagct caaattcctc agcgtcttgt tcttggggaa catccgccag
      661 ctctgcctgt ccattagccg cgcgaatcct cgtgaacgtt tcgcgtcgca ccgctacacc
      721 cacggtaacg ataggtttag ggttcaacag caactcagct aattccctat ccgcccacca
      781 agagaatgtt gctgcatcag cgggagcccg cccttgtcga taaatctccg aagcacccgt
      841 cacacgcgca gatttttcag cagcagccaa attaataatt gcggaggata gcgtgtttgc
      901 cttcatgtgt tctaagtttt gtcagaaccc cgcctaattt tacttcgagt ctgttcaacc
      961 ggcgctgcag gagacatcag cgacaccaca agcgccacca gcagtatccc tgccaccact
     1021 cccagcgaaa tatgcaccgg aacgtgaacc cagggctcca caatcatctt ccctccaata
     1081 aaaaccagca caccggcgag ccccaccgtc aaataacgca aacgctgcaa agccccagca
     1141 agcaaaaaat aaatagcacg caaccccaaa atcgcaaaca cattcgaggt gtacacaata
     1201 aacgcgtcgt gcgtgatgcc aaaaatcgca gggatcgaat ccaccgccag cgtgatatcg
     1261 gtaatttcca cgaccagcag tactagaaat aaaggcgtcg caaataattg tttgttcgaa
     1321 cgcacaaaaa atcgctcgcc ctgatattgc cgagttaccc gcaaatgcct gctcgccaac
     1381 tggaaaatct tgttttgctc cggacgcgtc tcccgcgtgc gaatcaaaag catgtgcacc
     1441 ccggcgtaca ctagaaacgc cccgaacaca tacatgatcc acgcaaaccg ggacaccagc
     1501 gccgcgccca tcgcaatcat gatgccgcgc atcaccagcg cgcccagcac gccccaggcc
     1561 agcaaacgat gttgtacccg ttcatcgaca gaaaatgctt ggaagatcac tagaaataaa
     1621 aacaggttgt cgatgctaag agctttttca atcaggtatc cagtgaagaa ttccagggct
     1681 ggctgcctgc ccagaaacca aaacacgccc acaccaaaaa atatcgacag ggcgacccac
     1741 cctgcgctcc agatcgccgc gtcgcgcagc ctgattctgt gcggcctgcg atgaaacaca
     1801 cccagatcca gcgcgacagc caccaacaca aaaatgttaa agaaaatcca gcgcgagagg
     1861 gtacccatat tcgcgttggc ttatttcaag gagcggaagc ggctgcgcgt gcgtccgtcg
     1921 cgttcaagtt gttcaactcc actcgcgtgg tctgccccgg ctgaatcgcc accggaacat
     1981 cccaccttac tcgcgtatcc cccgcgttgg catccagatt cagcgaactt acccagtaac
     2041 tccccggcgg caatcccgaa acccccgcgc gtccatccag atccgtatcc gccttggcca
     2101 ccagatattt tgtttgcgct acgtttggcg cattgtgctg cacgtttttc ttatgctcgc
     2161 tttcaagttt cgcccatttt cgctgcggat tcgcggcatc catctccagt tccatgccgc
     2221 tcatcgtttc cggatgcgcg ccgataaatt tccgcagcgc ggcgtagtat tgctcttttt
     2281 gcttttcgta tttctctgga tgctccgttt tatccgcatc cttatacttt ggtttgggaa
     2341 tcccgttggt gaccccgccg ctgttcgaac gctggtacgc cagcaaaaat tccggcacat
     2401 gcagcacgtt gtccgccttg agggccttgt ccagtcccgg catggtcaaa tccagaatct
     2461 catgagcttt cagccattct ttaagctcag gagaaacctt cagatcatca ataaatttgt
     2521 cgcgagaggg catcgcatcg tcggcttcca cttccttcgc aaccgccgca tagcttttgc
     2581 ttagaacgta aaaagtgaat tgccgcaccg gctccggccg cgccgccgta ggcgtgatct
     2641 tggccgtgag ctgcaacccc ccggtacctg tagccgtttg cgcgcatgca ctagtactga
     2701 tcaacaatga gccaagcaac acggccagta cggccattcg cgttttcatc attcgcaaat
     2761 cgtaacagat tgaagcccag ccgacgcatt gaacatccac tgtgttgctt tggtgacggg
     2821 ccatgctacg gaatgaggca tcagccaacc gggagattgg cattcacgct actggcacga
     2881 aacgccgttt ttaggtttta ttagtagagg tggaatgtaa cttcgatctg tacgcgacac
     2941 gcgacagctt tgccgccagg ccccaaagcc gcccgcattt tccaattgcg cacctgcgca
     3001 agcgcctttt cttccagccc aagtccaggg cctttaatca ccacaggatt agtcacgcgc
     3061 ccatccgctg ttaccgtcac atccagtaaa accgtgccct gatattttgc tttgcgcgct
     3121 tcatccgaat attccggctg cgggcaatag atacattgtg gtgaacccac tccgttcaca
     3181 cccgcgcgga aagccccgcc gcccgtgccg gcggtttcgc cagggcccaa ccccgcccca
     3241 gtcccgctgc caatgccgcc gctcgtgcct acaccgatcc caccgccacc gcccggtccg
     3301 cccgacatgg ttaccgcgcc cgccatcgga tcacccatat ttgtcagcgg ggggttcgca
     3361 atcttaagtt ccggcggccc gagcagcgtc ggatccatag gcaacttcgg ggccggattc
     3421 ttgagtgtcg cctccggcgg agtaaattgc gtatacgcaa atttcggagc acggcccttc
     3481 gtgggaggtg taatcgaccg gtcgccgccg ccaccaccgc cgccagcctt cttcgttccc
     3541 gcaggcaact tcgcgatgta cggcgaaata tccaacggcg tcgaatcaaa cttgttttta
     3601 gcttgtgtcg agctaggcaa aatatgcgtg aaaagaggaa ccaccagcaa agtaataatc
     3661 ccgacgtgca gcccaatcga aattatctgc gtccacccaa aattctcgtc cttcgaccag
     3721 atatccttaa cttttacagg cttgctcgtc cactgcgcag gagcctgctt caccggaaag
     3781 aacaattcct taagacggtc tccgaaccca ccgaaccccg cgccccaagc cacttccagc
     3841 cggctattcg aagcaccttt tggaactgga ccgcccttaa gaaccgtttt caaattctct
     3901 ttaaatccgt cgccgtaatt ttctggcatc agcggagaat gaacatctcc gctcaatttc
     3961 accggacgtt ccgtaagaaa ttgtttgagg ttgccccaga aaccatccgt tacgaatcgg
     4021 ctgggcgtcc cgaatcgcgg cagcggaact gccggagggc ctcccggcgt ggatgccgtc
     4081 ggatcgctgg gtttcgtcgg ctgcggtggc aaaagattga tcattctacc cggtaccgtc
     4141 ttctctgggt ttagacgcaa ttgttcgcga ttaagcgtgc cagccggtta tgattcgcgt
     4201 tgcccaggaa aggttgcgcg agccctgcgg ctgacatcga ctgctattca ttgtaacata
     4261 cgcatttaac cagctttccg atgtttcccg tcatatacca cttcgagtaa cagggacgcg
     4321 gtataaatac ggggaaaata ggcatttatc gcgctggcca ccggctctgg gaacaatgtt
     4381 tccgcgccat cggtgaccgc cagcgacaaa aggggacttt ccatttctca gctcatggcc
     4441 gaaccactgg aattaaagaa aactctaaac ctgcccaaga ccgattttcc catgaaagcc
     4501 agcttgccgc agaacgagcc taaacagctc gctgcctggg aagaggccca tctctacgaa
     4561 aaaattcttg agactcgcaa aggcaagcct ctttttgttt tgcatgatgg tcctccttat
     4621 cccacgggaa ctattcatct cggtaccggt ctaaacaaaa tcctgaaaga catgatcgtc
     4681 aaatccgaaa gcatggccgg tcattacgcg ccctacgttc ctggatggga ctgccacggt
     4741 ctgcccatcg agacccaggt cgaaaaagaa ttgggcggca aaggaaaagt ttccgccccg
     4801 gaatttcgta agctttgccg ccaattcgcg aatcgttacg tggaacagca caagcgagat
     4861 ttcaaacgcc tgggtgtctt cggtcgctgg aacgatccgt acctcaccat gaattttgat
     4921 tacgaagcca ccatcgcggg cgcttttctg gagttcatgg aaaaaggata cgtttatcgc
     4981 ggacgcaaac ccgtctactg gtgcctccac gaccgcaccg cattagccga agcggaagtc
     5041 gaatacgaag atcacaccag cccatccgta tgggtcaaat tcgctatcgc cgatggacag
     5101 aaacccggca acgaagtcag cgccgtcatc tggaccacaa ctccttggac tcttcctcat
     5161 aaccgcgcgc tcgctttt
//
LOCUS       DATCBB010000799         1372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_251720, whole genome shotgun sequence.
ACCESSION   DATCBB010000799 DATCBB010000000
VERSION     DATCBB010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251720"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VII37_08865"
     CDS             complement(<1..1362)
                     /locus_tag="VII37_08865"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HEY5069889.1"
                     /translation="MRFERSGPKVLLVQENLDYRAISDDQAERRAVHDSFAESVLAGF
                     AVSAEEKERVLVDATDFFLRDGHDIPATLRKTKQGTYHLDPARCAIYLPLTKNFPMNT
                     EVESILTFAGDEPGAWVKQVTPSPESITVREHHSFVQLPPPGYKPRVYDPRASFFGIS
                     YMDYATPVSEPIVKRYIARHRLEKKDPAAATSEPVQPIIYYLDRGAPEPIRSALLEGA
                     RWWNQAFEAAGYKNAFRVELMPEEADPMDLRYNVIQWVHRATRGWSYGSTVIDPRTGE
                     IIKGHVTLGSLRVRQDYLIAEGLLAPYEQGKPVSPRMLEMALARLRQLAAHEVGHTLG
                     LEHNYSASTVSRSSVMDYPPPVVTLGSDGVPDVSNAYATGIGEWDKVSITWGYREFPT
                     GADEHAELNKIISDALSGGLRFLTDQDARPPGSSSSLAHLWDSGSNAVDELNRLMQVR
                     AAAL"
BASE COUNT          304 a          395 c          381 g          292 t
ORIGIN      
        1 caacgctgcg gcgcgaactt gcatgaggcg gttcaattca tccaccgcat tggagccgga
       61 atcccataga tgcgcaagac tgctcgagga tccgggcggg cgagcgtctt gatcggtcaa
      121 aaaccgcagg ccgccactca gcgcgtcaga aataatttta ttcagctccg cgtgttcatc
      181 tgcaccggtg ggaaattcgc gataacccca cgtgatggat actttgtccc attcgccaat
      241 gcccgtagcg taagcgtttg aaacgtcagg cacgccgtcg ctgccaaggg taaccaccgg
      301 gggtggataa tccatcactg acgagcgact caccgtgctg gcggaataat tgtgttcaag
      361 ccccaaggtg tgcccgacct cgtgagccgc gagttgtcga agccgcgcaa gggccatctc
      421 cagcattctg ggcgaaacgg gtttgccttg ttcgtagggg gcgagcaaac cctcagcgat
      481 taaataatcc tgcctcaccc gcagcgaacc cagcgttacg tgccctttaa taatctcccc
      541 ggtgcgtgga tcgatcaccg tggatccata cgaccaacca cgcgtcgcgc gatgaaccca
      601 ctgaatcacg ttatatcgga gatccatagg atcagcctct tccggcatta attcgacgcg
      661 aaatgcgttt ttgtaaccgg ctgcttcgaa cgcctggttc caccagcgcg cgccctccaa
      721 aagcgcggag cgaatcggct ccggagcgcc gcgatcgaga taataaataa tcggctgcac
      781 cggttcgcta gtggccgcag cgggatcttt tttctctaac cggtgccgcg cgatatatcg
      841 cttcacaatt ggctcactta ccggcgtggc gtaatccatg taactaatgc caaagaaact
      901 cgcgcgggga tcgtaaacgc gcggtttgta acctgggggc ggaagctgca cgaaggaatg
      961 atgctcgcga acggtgatgg attctgggga aggggtgact tgtttcaccc acgcaccggg
     1021 ttcgtctccc gcgaacgtca gaatggactc aacttccgta ttcatgggga aattcttagt
     1081 gagcggaaga taaatcgcgc aacgtgccgg atcgagatgg tacgtgcctt gtttggtctt
     1141 gcgcagcgtg gccggaatgt cgtgaccgtc gcgaagaaaa aaatcggtgg cgtccaccaa
     1201 aacacgctct ttttcttcag cgctcaccgc gaatcccgcc agcaccgact ccgcgaacga
     1261 atcgtgtacc gcgcgccgtt cggcttggtc atcgctgatc gcgcggtaat ccaaattttc
     1321 ctgtactaac aaaacttttg ggccgctgcg ttcaaagcgc acgatgcggg tc
//
LOCUS       DATCBB010000800         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_284660, whole genome shotgun sequence.
ACCESSION   DATCBB010000800 DATCBB010000000
VERSION     DATCBB010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284660"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VII37_08870"
     CDS             complement(<1..256)
                     /locus_tag="VII37_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069890.1"
                     /translation="MSPTTNENERMNRPLDVVDSQIADLLRDEARRQATGLELIPSEN
                     LVSEAVLEAMGSIFTNKYAEGYPGKRYYGGCEYADKVEQLA"
     gene            complement(353..1735)
                     /gene="mgtE"
                     /locus_tag="VII37_08875"
     CDS             complement(353..1735)
                     /gene="mgtE"
                     /locus_tag="VII37_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY5069891.1"
                     /translation="MPVTVSPSEWTDLVARQRWEELRSKLERTHVSDIAEMLTALPPK
                     ERAIVFRLVARDRAAEVFGYLPPSEQKELIHSLTADEVKRMLNEMRPDDRTRFFGEMP
                     AEVTRKLLEELSPEELKESRELLGYPLESVGRYMTPHYVSIAPDMTAGEALEHIRNIG
                     QKSETLNVVYILDVNGKLLEDIRLGSLVLADPQTKVKNIADPAMVSLHAMDDRETALG
                     LFEKYDRVALPVTDAQGYMLGIVTVDDMLDVAQAEATEDIQKLGGSEALDAPYNQVGL
                     WEMIRKRGGWLSALFLGEMLTATAMSFFEGEIAKAVVLALFVPLVISSGGNSGSQAAS
                     LIIRALALRELKLRDWFRVFRREFISGLALGFMLGVIGFFRIVLWQHLHLTNYGTHYL
                     LLAFTVWASLIGVVMFGTLAGSMLPFILRGLGFDPAASSAPFVATLVDVTGLCIYFSV
                     ALLILHGTLL"
     gene            1879..>2428
                     /locus_tag="VII37_08880"
     CDS             1879..>2428
                     /locus_tag="VII37_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY5069892.1"
                     /translation="MAQKKFRDLTEREILALAIASEEEDGRIYGEFAEGLRDSYPATA
                     QMFEEMREEEVVHRDRLMAMFRERFGDRIPLIRRENVKGFLTRKPTWLTRPLGVNVAR
                     RQARVMEMEAARFYDKAVARITDVPIRELLNKLADEERKHSAVAGDLEEKHLTENARA
                     EEDESHRKLFLLQVVQPGLAGLM"
BASE COUNT          568 a          712 c          632 g          516 t
ORIGIN      
        1 tggccagctg ttccactttg tcggcatact cgcagccgcc gtaatagcgt ttacctggat
       61 aaccctcggc gtatttgttc gtgaaaatgg atcccatggc ctccaacacc gcttcggaga
      121 cgagattttc ggatgggatc agctccaagc cagtggcctg ccggcgcgct tcatcgcgca
      181 acagatcggc aatttgcgaa tcgacaacat ccagcggccg gttcatgcgc tcattttcat
      241 tggtggtagg actcatgatc actcctgaat gcggaagggc aacaggcgaa attctatcgg
      301 accgcgcggc accgcgcaaa cacgcacaaa ctcggcggtc atcgcttttc agttacagca
      361 atgttccgtg caggatgagc agggccacgg aaaaataaat gcacaagcct gtaacatcca
      421 ccagcgtagc tacaaacggc gcagaactgg ccgccgggtc aaatcctagt ccgcgcaaaa
      481 tgaacggcaa catgctgcct gccaatgttc cgaacatcac tacgccaatc aaactggccc
      541 acaccgtaaa ggccagcagc aggtaatgcg tgccgtaatt cgtgagatgc aggtgttgcc
      601 acaacacaat gcgaaaaaac ccaatcacac ccagcatgaa ccccagcgca agtcctgaaa
      661 taaattctct gcgaaacacc ctgaaccagt cgcgcaattt taactctcgc agtgccagcg
      721 cgcgaataat cagcgatgcc gcttgcgacc cggagttccc accgctgctg atgaccagcg
      781 gcacaaacaa cgccaacacc accgctttgg caatttctcc ctcgaaaaat gacatcgccg
      841 tggccgtcag catttcgccc agaaacagcg ccgagagcca ccctccccgc ttgcgaatca
      901 tctcccacag gccaacctgg ttgtacggcg catccagcgc ttcggaaccg ccgagctttt
      961 gaatatcctc ggtggcttcc gcctgggcca cgtccagcat gtcgtcaacg gtcacaatcc
     1021 ccaacatgta gccctgcgca tcggtcaccg gcaacgccac gcggtcatat ttttcaaata
     1081 ggccgagcgc cgtctcgcga tcgtccatgg cgtgcaaaga aaccatcgcg ggatcggcaa
     1141 tgttcttcac tttagtctgc ggatcggcca acaccagtga acctagccga atgtcttcga
     1201 gcaattttcc attaacatcc aggatataaa cgacgttcaa agtttcactc ttttgcccga
     1261 tgttgcgaat atgctcgagc gcttcgccag cagtcatgtc tggtgcgatc gatacgtaat
     1321 gcggggtcat gtagcggccc acgctctcga gcggataacc gagcaactcg cgcgattctt
     1381 tcaactcttc cggcgaaagc tcctcaagaa gcttccgcgt aacttccgcg ggcatttcgc
     1441 cgaaaaaccg cgtgcggtcg tcgggacgca tctcgttgag catgcgtttc acttcgtccg
     1501 ctgtgagtga atggatcagt tctttttgct cgctcggcgg caggtagcca aaaacctccg
     1561 cggcacggtc gcgcgctacc aatcgaaaca cgatggcgcg ttctttcggc ggtaacgccg
     1621 ttaacatttc cgctatatcg gaaacgtgcg tgcgctccag tttgctgcgc aattcttccc
     1681 agcgctggcg cgcgaccagg tcggtccatt ccgacggtga aacggttaca ggcattggca
     1741 ctctcactct tccgttgaag tttgaatcag cctgcctatt atgcggtctt ctccaggacc
     1801 agagactaca ttcccggtgg gtgacttatt ctctctgttt gtgcgattct tcttccccac
     1861 aggagtatcc aggacaagat ggcgcagaaa aaattcagag acttgacgga aagggaaatt
     1921 ctggccctgg ccattgcttc cgaagaagaa gatggccgca tttatggaga gtttgccgag
     1981 ggtttgcgcg attcttatcc cgccaccgcg cagatgtttg aagagatgcg cgaagaagaa
     2041 gtggtgcatc gcgaccggct gatggccatg tttcgcgagc gctttggcga tcgcatcccg
     2101 ttgattcgcc gcgaaaacgt gaagggattt ttgacccgca agcctacttg gctgactcgc
     2161 cctctcggcg tgaacgtggc cagacgccag gcgcgcgtca tggaaatgga agcggcgcga
     2221 ttttatgaca aagcggtagc gcgcatcacc gatgttccga ttcgcgagct tttgaacaag
     2281 cttgccgatg aagaaagaaa acactcggcc gtcgcgggcg atctggaaga aaagcatcta
     2341 accgaaaacg cgcgcgcgga agaagacgaa tcgcatcgca agctgttttt gctgcaggtc
     2401 gtgcagcccg ggctagcagg attaatgg
//
LOCUS       DATCBB010000801         3433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_123494, whole genome shotgun sequence.
ACCESSION   DATCBB010000801 DATCBB010000000
VERSION     DATCBB010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123494"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..963
                     /locus_tag="VII37_08885"
     CDS             76..963
                     /locus_tag="VII37_08885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5069893.1"
                     /translation="MKVGRYEILETIGTGANGSVVRGHDPMIGRLVAIKLLPAELAKD
                     EARNRFMQEARVVGQLSHPSIITLHDMGIEESTSTPYLVMEFLSGQSLDRILEKGAVP
                     TMRACAWVADVAGALATAHRKGVIHGDVKPANILITEDGRVKLMDFGMARVAAREQAS
                     MALTGTPAYWCPEQIMGKPQDGRSDLFSLGVVLYELITRQRPFDADSLQGVCNRILSH
                     EPLPVSNLNPSLSTTLSEVVARCLSKDPNTRYPSGEELANDLYPFARRKVTGQGIGQL
                     IKTQSKTFSSRTVKYPQSA"
     gene            1048..2982
                     /locus_tag="VII37_08890"
     CDS             1048..2982
                     /locus_tag="VII37_08890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5069894.1"
                     /translation="MIPIGGSLGENVSVAQAEGSLAIREKLMTTPQGRYEFIETLGTG
                     ATSRVDKARDTLIGRVVALKTFLHGFGSGDVQKQFLREAQIIGRLAHPFIVGLYDVGT
                     NAEGVPYFVMEYVDGKTLEKVLDDGPLPLEKVALWATDLAAAIARAHRAKVIHGDIKP
                     ANILITKEGQVKLGDFGIARFATQVSNSGVLMGTPAYLSPEQIQGNAQDTRSDLFSLG
                     IILYQMTTGVQPFSGSSVSAVCAQIVAITPPPPSHHNPSLPPAFDRIVVRCLAKDPAD
                     RYATAEALGASLYPFAHALELAPAAPEKPSTAAAFVARAEKWAITAGKALKAEAQEQS
                     RRLSSLITAIWTSDSAWWNRPMQRKDLWAAGTAAVLLIGLVPMARALRNRAHAQPVVS
                     AAALSGASGAPAASNANSQAPITSNASMINISSSLVGDTAVAGADAGAKSSVNFQGDK
                     TKTVVTASQGLDRHAVTTAKTRQLSRQAATKTATALPESLPVYGPSLPAIVPVVESAP
                     LKAAVAQTTLRINIVSAVTDQTTLAVFSGDELLLTTPLKPQQVGDTLRFNCPIVAGEH
                     ALRVVLYRADKTVFMQKENKSELHADGANTMEVHVNRKSKMLVKHETSLEVVWPSRTT
                     SSSSAEANQGFKPAGALALR"
BASE COUNT          749 a          863 c         1074 g          747 t
ORIGIN      
        1 taatggaccc agcctgtcac gaagactcct cgagagattc ctggaaaggc cgccgtgtca
       61 attaggaagg catccatgaa ggttgggcgt tacgaaattt tagaaaccat cggtacaggc
      121 gcgaacggca gtgtggtgcg cgggcatgat ccgatgatcg ggcgtctggt agccatcaag
      181 ttacttcccg cggaattggc aaaggacgag gcgcggaacc gctttatgca agaagcgcga
      241 gtggtggggc aattgtcgca cccttccatc attacgctgc acgatatggg cattgaagag
      301 tccacatcga caccgtatct cgtgatggaa tttctaagcg gccagtcgct ggaccgaatt
      361 ttagaaaaag gcgccgtacc tacgatgcgc gcgtgcgcct gggtcgcgga cgtagccggc
      421 gctttggcga cggcgcatcg caaaggggtg attcacgggg acgtcaaacc cgcgaatatt
      481 ttaatcaccg aagatggccg ggtgaaatta atggatttcg gcatggcgcg ggtggcggcg
      541 cgagaacaag ccagcatggc gcttacgggc acgccagcat actggtgtcc ggaacaaatc
      601 atgggcaaac cgcaggacgg gcgatctgat ttattttcgc ttggggtggt cttgtatgaa
      661 ctgattacca ggcagcgtcc cttcgacgca gattcactgc agggcgtgtg taatcgaatc
      721 ctttcacacg aacccttgcc ggtatcgaat ctcaatccgt cgctttcaac cacgctaagt
      781 gaagttgtgg cgcgatgcct gtcgaaagat ccaaatacgc ggtatccgtc gggagaggag
      841 ctggccaacg acttatatcc ttttgcgagg cgtaaggtta cggggcaagg gatcggccag
      901 ctgattaaaa cgcagtccaa gactttcagt tctcggactg tcaaataccc gcagtcagca
      961 tagtgattgt tagtgaagag ctaactaggg tacttccttt aggccgtagg gacagggtac
     1021 gcgagtttta cacactggga ccattgggtg atacccatcg gagggtcgct cggggaaaat
     1081 gtttctgtag cacaggcgga aggctcgctc gctatacgcg aaaaacttat gactactccg
     1141 cagggacgtt acgaatttat tgaaacgctg gggaccgggg ctaccagccg cgtggataag
     1201 gcccgtgaca cgctgatagg tcgcgtcgtt gcgttgaaga cgtttctgca cggtttcggt
     1261 tcgggggacg ttcagaagca attccttcgt gaagcgcaga tcattggacg actggcacat
     1321 cccttcatcg tcggacttta cgacgttggc acgaatgccg agggagttcc ttacttcgta
     1381 atggagtatg tggacggaaa gacgctggaa aaagttcttg atgatgggcc gctaccgctg
     1441 gagaaggtgg cgttgtgggc cacggacctt gcggccgcga ttgcgcgcgc gcatcgcgcg
     1501 aaagtcatac acggcgatat caagccggct aacattctga tcaccaaaga gggacaggtt
     1561 aagctgggtg attttggtat cgcgagattc gcgacgcagg tgtccaattc cggggtgctg
     1621 atgggcactc cggcttattt gtcaccagag cagattcagg gaaatgcgca agacacgcgc
     1681 tcggatcttt tttcgttggg gatcattctg tatcagatga ctaccggcgt gcagccgttt
     1741 agtggcagct cggtgagtgc ggtgtgcgcg cagattgtgg cgattacgcc gcctcctcct
     1801 tcgcatcaca acccaagttt gccgccagca tttgatcgca tcgtggtgcg ttgcctggcc
     1861 aaggatcccg cggaccgata cgcgacggca gaggcgctag gcgcttcgct gtatcccttt
     1921 gcgcacgcgc tggaattggc gccggctgcg cccgaaaaac catcgactgc tgctgcgttt
     1981 gtcgcgcgcg ctgaaaagtg ggcgataacg gctggaaaag cattgaaggc cgaggcacag
     2041 gaacaatcgc gacggttgag ctcgttgatc acagcgatat ggacttccga ttccgcgtgg
     2101 tggaaccgtc cgatgcagcg caaagattta tgggcggcgg gcacggcagc agtgttgctg
     2161 atcggactgg tgcccatggc tcgcgcgtta cgcaaccgcg cgcacgctca gccggtggta
     2221 tcggcggcag ccttgagtgg cgcaagtggt gcgcctgctg cgtcgaatgc taattcacag
     2281 gcgcctatta cttcaaatgc ttcgatgatt aacatcagct cgtcattggt gggcgatacg
     2341 gcggttgctg gcgcggatgc gggcgcgaag tcgtcggtga acttccaagg cgacaaaacg
     2401 aagacggtgg taacggcgag tcaggggttg gaccgccatg cggtgacgac agccaagacg
     2461 cgccagttaa gccgacaggc ggcgacgaaa accgctacgg cattaccaga gagcctcccg
     2521 gtttatggac cgagcctgcc ggcgattgtg ccggtggtgg aaagcgcgcc actgaaggcc
     2581 gccgtcgcgc agacgacatt gcgtatcaac atcgtatcgg cggtgacgga tcagacgact
     2641 ttggcggtct tttctggcga tgaattgtta ctgacgactc cattgaagcc ccaacaggtc
     2701 ggcgacacgt tgcgcttcaa ttgcccgatt gtggcgggcg aacacgcgct acgagtggtg
     2761 ctgtatcgcg cggataagac ggtgttcatg cagaaagaaa ataaatctga actgcatgcc
     2821 gatggcgcaa acacgatgga agtgcacgtg aatcgcaagt cgaagatgtt ggtgaaacac
     2881 gagacttcgc ttgaggtggt gtggccgagc aggaccactt cgtcgtcgtc tgctgaagcg
     2941 aatcagggat tcaagcctgc tggggcgttg gcgctgcgtt gattttgcag ttcgctttgc
     3001 aattcaaata aagaaaattc caatctatga accgaggtcg cggaaatgac catgagctta
     3061 ccagcccatt ttcaccgcat ggatgtaacg cgaccgcacc tttggtggat cagctgcgaa
     3121 ctgtgtcagg gaacgggtgt cgctcagctt caatggaata gggctgtatg gtgtaccgag
     3181 tgcgatcgca gcggcaagat tcgcattgtg gtttaccggc cggattcgct taaagtggcg
     3241 cagcgacgac tggggatcgc tacttgcgtc gcgttcgcgg cgctcgcttg ctacctaatt
     3301 ttttgagcta acgtggtttc tcgcccaagc cgcaatcctg aactgctatc aggaggtggg
     3361 ctttggcgat tcgatagcta ctcgtagcgc agggcttcga cggggtcgag cttggcggct
     3421 ttcatggctg gcc
//
LOCUS       DATCBB010000802         1213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_221123, whole genome shotgun sequence.
ACCESSION   DATCBB010000802 DATCBB010000000
VERSION     DATCBB010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221123"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1213
                     /locus_tag="VII37_08895"
     CDS             <1..>1213
                     /locus_tag="VII37_08895"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5069895.1"
                     /translation="EYIEIGDSNGGIILDISEGGMAVASAQALVGNQTLRFRFQLPRS
                     LETIETSGEINWIGETKKRAGVRFVDLPLAAREQIHKWIDEQTSGHSNGDQKPNGSAV
                     GSASPKIPRELPRYEPINRGAIRQESIIPRGQDHEDSTNLNPFSSTLSSPLDEPEVDE
                     AEAEDEEKATADSNAKPLSEKRAQTRFPMTASTYVQLSDGNGGLMANLSKTGFCVRAA
                     KTLETDHLPVVRFQLPNARDFVESSAWIVWKSHSKKAAGARFENLSAEAQAQIAQWID
                     AQPLPKTAPTKNLPSKNTLVPVKSPLEQREKISPSSKISAPVTPVPATPVPATPVPLT
                     LMAPDSVPIPAYISPLAISLNAKPAVSSSNTPSAAPPLSKASPSAPPSKAPPAPTVKP
                     PAPLAAVPRMVS"
BASE COUNT          296 a          379 c          291 g          247 t
ORIGIN      
        1 cgaatacatc gaaatcggtg acagtaacgg cggcattatt ctggacatca gcgaaggcgg
       61 catggcggtc gcctccgccc aagccttggt cggcaaccaa accttgcgct tccggtttca
      121 gctcccccgc tctctcgaaa cgattgaaac ttccggcgaa attaactgga ttggcgaaac
      181 taaaaaacgc gccggtgtgc gtttcgtcga tctccctctc gctgcccgcg aacaaattca
      241 caaatggatc gatgagcaaa cttctgggca ctccaatggc gatcaaaaac cgaatggttc
      301 agcggtgggt tcggcatcgc cgaaaatccc tcgtgaactt ccgcgctacg agcccatcaa
      361 tcgcggagct atacggcaag aatccatcat tccccgcgga caggatcatg aagattcaac
      421 caatctgaat cctttttcgt ccacgctctc cagtcctctc gacgaaccgg aagtagatga
      481 agccgaggcg gaggacgaag aaaaggccac cgccgattcg aatgccaagc cgctttctga
      541 aaagcgcgcg caaacccgtt ttcccatgac cgcctctacc tatgtgcaac taagcgacgg
      601 gaacggcggc ttgatggcca acctcagcaa aactggattc tgcgttcgcg ccgcgaaaac
      661 gttggagacc gatcacctac ctgtagtgcg ctttcagctg cctaacgccc gcgatttcgt
      721 agaatcatcg gcctggattg tttggaaaag tcattcaaaa aaagcagctg gcgcccgttt
      781 tgaaaatttg tctgcggagg cgcaagctca gatcgcgcaa tggatcgacg cgcaaccgtt
      841 gccgaagacc gcgccaacca aaaatttacc ttctaaaaat acgcttgtgc cggtcaaaag
      901 tccgttggag cagcgcgaaa aaatttcgcc ctcatcgaaa atttcggcgc cggtaactcc
      961 ggtacccgca actccagtgc ccgcaactcc cgtaccccta acattgatgg ctccagactc
     1021 ggtgcctata ccggcttata tttcaccatt ggctatatcg ctgaacgcaa agcccgcggt
     1081 ttcatcctcg aataccccgt ctgccgcgcc acctctgtca aaagcgtccc ccagcgcgcc
     1141 gccaagcaag gcaccacctg ctccgactgt gaaaccgccc gcgccgctcg ctgcagttcc
     1201 acgtatggtt tcg
//
LOCUS       DATCBB010000803         1175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_25927, whole genome shotgun sequence.
ACCESSION   DATCBB010000803 DATCBB010000000
VERSION     DATCBB010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25927"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1175
                     /locus_tag="VII37_08900"
     CDS             <1..>1175
                     /locus_tag="VII37_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069896.1"
                     /translation="DPLIQQLDPTQKNAAQLLAQLAIWTDIGYSGPPLNQLLRLFKDA
                     GGPRSQLPIVDYVCLRIAEGMTAMADEAMEKAIGHFDFVLSIGDVLNDPFLLSIVYFW
                     KGRCLRRRGEYDEALIYTGKGRDLAIALKHLRMAAVMQVLEGWLLFQQGKWKEAVRIS
                     QAAERVLADTDDYVTLGNIQSFYGRMARREGRFDEAIKFFENAIRHYRKRDPQHPNLA
                     RSLANMALAKRGIALQLQKRIDRDAERRRKNSTSKQAKSKASSNETAESDSHNPAYRG
                     QLAQLRREALEHLDAAQSIYQQRPNHHGLGTVYLNAAYIHLDSGDFQRAEEEVSSAFQ
                     AAEEKQDYILMVRARILQCMIENGKVEEEIGGATDPSSHARRALEFSQQAIDLAKHT"
BASE COUNT          284 a          324 c          331 g          236 t
ORIGIN      
        1 acgacccgtt gattcagcag ctcgacccca cccagaaaaa tgcggcgcaa ctcctggccc
       61 agctcgccat ctggacggac attggctaca gcgggccgcc gctcaatcaa cttcttaggc
      121 tttttaaaga tgcaggagga ccgcgatcgc agcttccgat tgtggattac gtctgtttac
      181 gcattgccga gggcatgacg gcgatggccg atgaggccat ggaaaaggcg atcggtcatt
      241 ttgatttcgt cctaagcata ggcgacgtat tgaacgaccc cttcctgctt tccatcgttt
      301 atttctggaa gggccgctgc ctgcgccgcc ggggcgagta tgacgaagcc ctgatttata
      361 cgggaaaagg aagggatctg gcgatcgcgc tgaagcatct gcggatggcg gcggtaatgc
      421 aggtgctcga aggttggctt ttatttcagc agggaaaatg gaaggaagca gtccgcatct
      481 cgcaggcggc ggaacgggtg ctcgccgaca ctgatgatta tgtgacgctc gggaacattc
      541 aatcctttta cggaagaatg gcgcgccggg aaggccgttt cgatgaagcg atcaagtttt
      601 tcgagaacgc cattcgccat tatcgtaaac gcgatccgca gcatccgaat cttgcgcgtt
      661 cgctggcaaa catggcgctg gccaagcgcg gaatcgcgct tcagttgcag aaaagaatcg
      721 accgcgatgc cgaacgccgc cgtaaaaact ccaccagcaa gcaagcgaaa tccaaagcat
      781 ccagcaatga aacagcggag tccgattccc acaatccagc ctatcgcggc caattggccc
      841 agcttcggcg cgaggctctc gaacatctcg atgctgccca gagtatttat cagcagcgcc
      901 ccaaccacca cgggctgggc actgtttact taaatgccgc gtacatccac ctcgatagtg
      961 gagactttca gcgcgccgaa gaagaagttt cctccgcatt ccaggctgcc gaggaaaagc
     1021 aagattacat tttgatggtc cgagcccgca tactgcagtg catgattgaa aatggcaagg
     1081 tggaggaaga aatcggtggc gcaaccgatc ccagcagcca tgcgcgccgc gctctggagt
     1141 tcagccaaca ggcgattgat ctggcgaagc atact
//
LOCUS       DATCBB010000804         1352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_415396, whole genome shotgun sequence.
ACCESSION   DATCBB010000804 DATCBB010000000
VERSION     DATCBB010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415396"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VII37_08905"
     CDS             <1..180
                     /locus_tag="VII37_08905"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5069897.1"
                     /translation="NPMGAAALTLSGGEYAIHGTNQPKSVGGFVSYGCIRMFNRDIVE
                     LYRMVDVGTPVVVLK"
     gene            721..942
                     /locus_tag="VII37_08910"
     CDS             721..942
                     /locus_tag="VII37_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069898.1"
                     /translation="MIQSPSTNLNTGEQPHVGYSRFSSREKALKYAGERLAPDHKAIR
                     MYGPNGVSIDENEIENEIKERRANRPSAA"
BASE COUNT          317 a          379 c          364 g          292 t
ORIGIN      
        1 aatccgatgg gcgcggcggc gctgacgctg agcggcggcg aatacgccat ccacggcacc
       61 aaccagccga aatcggtggg cggtttcgtg tcctacggct gcatccggat gtttaatcgc
      121 gatatcgtcg agctctatcg gatggtcgat gtcggcacgc cggtggtcgt gctgaagtaa
      181 ttgccgctga gccctcggca tcgccgttta cgctatttcc agcttttgca gagaagcgcg
      241 cggctgcgcg gctatgctgg cgcacgggtg ttgctgggag ctttctcatg tccgctttgg
      301 gtcaaaagcg gacaaaccgg ctcgcgccaa aatccacctt tgtccgctgt tgtccaaaag
      361 cagacaaacg cgggtccgat tggaatgtcc gctttgtgcc acaagcagac tcatgcactg
      421 caacatgacc ggcgcaaaaa gaaagaccgc ctcgcggcgg tctctctgat atccgatcaa
      481 agaacacttg attgggccta ggccgcaagc cgcgagtaag cggtaacgat ttcaaggcgc
      541 ggtggacggc ggagcgccgg atgagcgtct agtgcataca aggccccgcc tattaattag
      601 gagtcccgtg gagattagtt cctttgtcac ggaaccttac tctcgcctcc gatgttcccc
      661 ttttagagca caaagagggc aagggccatg acgacagccg gtcagggaag atggaagcta
      721 ttgatccaat caccctcaac taatctgaac accggcgagc agccccatgt tggctacagc
      781 cgttttagct cacgcgaaaa ggccctgaaa tatgccggtg agcgactagc acctgaccat
      841 aaggccattc gcatgtatgg cccaaacggt gtgtcaattg atgaaaacga aatcgaaaac
      901 gaaattaagg aacgccgcgc taatcggcca tccgctgcgt aacacaggca tttgctgcgt
      961 cagcagcctg gagagctgct cggctggatg agtctgggca attcgtttca caccacaaat
     1021 ttaaaaatta cgcaaaaaaa tatctagcgg cttgttctca acttgttgct taggatcaaa
     1081 atcggctcac aaacagaaag accgcctcgc ggctgtctct ccgaaatagt ctaccggcag
     1141 tgcagcaatt ccgctctgca atgtcgctgt tgggtcatta gcgaccgatc cattctgcgc
     1201 cagtgcctac caatgtccgc tatgcatcca atagcgaccg tcagccatcc cgcgacagac
     1261 tgacgctatg tcctatttgg tagacgtctt gagtgcgagg ttcgtgccgc taacgtggcg
     1321 cttcacgttt aataccaaat cgcgttaccc gc
//
LOCUS       DATCBB010000805         1090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_446680, whole genome shotgun sequence.
ACCESSION   DATCBB010000805 DATCBB010000000
VERSION     DATCBB010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446680"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..638
                     /locus_tag="VII37_08915"
     CDS             414..638
                     /locus_tag="VII37_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069899.1"
                     /translation="MHTIATAAAAVGRNKTAILRAIKAGKISVAKDENGEWQIDPAEL
                     HRIYPPLRSASMRGNDMQPHLHQSPLLTPV"
     gene            856..>1090
                     /locus_tag="VII37_08920"
     CDS             856..>1090
                     /locus_tag="VII37_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069900.1"
                     /translation="MIWILDKNAAVKLTAIARNSILSKSKHVPILQVGFAIALALAIV
                     VFVNLAIAHRTLTAQVVESTASTADDVINVGSIA"
BASE COUNT          246 a          313 c          277 g          254 t
ORIGIN      
        1 cggtattgtt gaggtgcggt cgtttcgatg cccgccggcg aagcacattg atctgttgcc
       61 gcaggatcag gatttctgct tccagcgttg ctctcgactt gaagagcgaa gccaggacgc
      121 cgagaatcag catgggcaga tcagtcatca ggtcgcgagc ttaccccgtc tgttctctgc
      181 gtccatgctg aatcagatat tcggtacaca caagtccaac gatctggcgc tattgtcgcc
      241 attcccatct tggctgggct gcatcataaa tacatccgga tatgattttc ggaaaggaca
      301 cccatccgcc ttaaaacggc tggtgccgaa gagcggggtg ctgcccccag acacgttttt
      361 ttaaccaggg cggttgcacc gtatctgctc actgaaacgg taggcaacac cccatgcaca
      421 ccatcgcaac agccgctgcg gcggtcggcc ggaacaagac cgcgatcctg cgggcgatca
      481 aagccggaaa gatttcagtt gctaaggacg agaacggcga gtggcagatc gaccccgccg
      541 aactgcaccg catttatccc ccattgcgga gcgcatccat gcggggcaac gatatgcagc
      601 cgcacctgca tcagtcgccg ttgctaacgc cagtttgagg atcacggctt gcgctcccac
      661 gccgccttaa aatccgccat cgcttcctcg cggctttcgc catagccgcg acgcataatc
      721 cagatattag ataacgtcgg cacaaaatcg gatttcggaa ataccgccgc gagtcacagt
      781 tcgagtcgta agagattttt taatctttcg taagtaacca agcctttgcg ttcattttgt
      841 attagcggct ccaccatgat ctggattttg gacaaaaacg cggcagtcaa attgacggca
      901 atcgcgcgaa attcaattct ttcaaagtca aaacatgttc cgattttgca agtagggttt
      961 gccatcgctc ttgcgcttgc cattgttgtc ttcgttaatc tggccatcgc gcaccgcaca
     1021 ctgactgcgc aggttgtcga gtccaccgct tctaccgctg acgacgtgat caacgtgggt
     1081 agcattgccc
//
LOCUS       DATCBB010000806         1059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_349945, whole genome shotgun sequence.
ACCESSION   DATCBB010000806 DATCBB010000000
VERSION     DATCBB010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349945"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VII37_08925"
     CDS             complement(<1..289)
                     /locus_tag="VII37_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069901.1"
                     /translation="MSKFFRESEFARLLELLAGILLMSFLVFILLVLPLQSKQAQNSA
                     PAEMVCGTINTAFTEGRCIHITFSGEVSGDKNFDRPFGPLRSRLNSEQGFRR"
     gene            complement(363..>1059)
                     /locus_tag="VII37_08930"
     CDS             complement(363..>1059)
                     /locus_tag="VII37_08930"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5069902.1"
                     /translation="GTGQSTIDGVIRATNVLIAGLKVVVAGYGWCGRGVAMRAKGLGA
                     DVIVTEIDPVKGIEAVMDGFRVMPMEQAVKEGDIYITVTGNKSVIRAEHFEKMKAGAI
                     VCNSGHFNVEIDIPALEKLSSGKREVRPLVDEYTLKGDRKVYLLGEGRLVNLATAEGH
                     PASVMDMSFANQALSAEYLRANYKTLKPQVYVVPEHLDKEIARLKLETMGHKLDKLTP
                     EQEHYLASWQEGT"
BASE COUNT          251 a          350 c          226 g          232 t
ORIGIN      
        1 gccgcctaaa gccctgctcg ctattcaagc gagagcgcaa cggcccaaac gggcgatcaa
       61 aattcttgtc gccggagact tctccgctaa aggtgatgtg aatgcagcgt ccttcggtga
      121 acgccgtgtt tattgtgccg cacaccattt cggcaggagc ggaattttgc gcctgtttac
      181 tttggagagg gagaactaac agaataaaca ccaaaaaact cataagcaga atgccggcga
      241 gcagttcaag caatcgcgcg aactcggact cgcgaaaaaa tttggacact ggagtcgcct
      301 gcaacgtcgg cggcagtgtc caatgaacgc ccctgccgcc gctacgcctc aaaaattact
      361 aactacgtgc cttcttgcca cgacgccaaa taatgttcct gctccggcgt cagcttatcc
      421 agcttgtgtc ccatagtctc cagcttgagc cgcgcaattt ccttgtccag atgttccggc
      481 accacataca cctgcggctt cagcgtcttg taattcgccc gcagatactc agcagagagc
      541 gcctggttcg caaaactcat atccatcacc gaagccggat gcccttccgc agtagccaga
      601 ttgaccagcc gtccttcgcc taacaaataa accttgcggt cgcccttcaa agtatattca
      661 tcaaccagcg gacgaacttc ccgcttgcca ctcgacaatt tctccagcgc aggaatatca
      721 atctccacgt tgaagtgtcc cgagttacaa acaatcgcgc cagccttcat cttttcaaaa
      781 tgttcagcgc gaatcacgct cttgtttccg gtcacggtga tatagatgtc gccttccttc
      841 accgcctgct ccatgggcat cacccggaat ccgtccatca ccgcctcaat gcctttcact
      901 ggatcaattt ccgtgacgat tacatccgcg cccaagccct tcgcgcgcat cgccacgcca
      961 cgcccgcacc agccatagcc cgccaccacc actttcaatc ccgcaatcaa aacattcgtc
     1021 gcgcgaatca cgccatcaat cgtcgattgc cccgtcccg
//
LOCUS       DATCBB010000807         1280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_446702, whole genome shotgun sequence.
ACCESSION   DATCBB010000807 DATCBB010000000
VERSION     DATCBB010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446702"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..>1280
                     /locus_tag="VII37_08935"
     CDS             147..>1280
                     /locus_tag="VII37_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069903.1"
                     /translation="MPDYMFLLESRLSPEQRAVLTRVQELSAAMGFNVYLTGGTVRDL
                     VTGASLRDLDFTVEGNPAKIARELEKGGAKTLVEDDKFRHIELLFSGDVEGSISAARD
                     DHYIRPGTRPEVRWSTIMEDLRRRDFSLNAVAISLNPASRGLLLDPTNGLSDIERAEV
                     RALTIHAFTNQPVRLLRVLRFAARMGFKIEARTQEWFDLAVERELPATITPEDAGAEL
                     RALAREERPAVVLKAWEDHDLLEAVNPLLAKKHPGYDAINRLSNVREDLFTAGLRPRL
                     ATPMLLAILARLKDREQSGVLSKAGYRSAESQEVLNFDKHIQTVQKELTGKKVGTPLE
                     AYKFLEKLPVDQIAYIMAESNNSGAVSKIRAYLNKWRPLRSGLP"
BASE COUNT          283 a          330 c          378 g          289 t
ORIGIN      
        1 gaagagcggt tgaagcaaat ttataaagcg catcatcccc gggcatgagc atgtttcaag
       61 cagatgccgc gggacaggcc ttgatgttgt agcaggtgct ttcgtgattt tcgcgcaaac
      121 atcctgaaga aagaagaagg agcgcaatgc ccgattatat gtttttgttg gaaagccggc
      181 tctcgccgga gcagcgcgct gtactcacgc gcgtgcagga actgtccgcc gccatgggtt
      241 tcaatgtata tctgactggc ggcacggttc gcgacctggt taccggtgcg tcgttgcgcg
      301 atctggattt cactgtggaa ggaaatcccg caaagatcgc gcgcgaactg gagaagggtg
      361 gcgccaagac gctcgttgaa gacgataagt ttcggcatat cgaactgctc ttcagcggcg
      421 acgtggaagg tagtatttcc gcggcgcgcg acgaccacta catccggccg ggtacgcgtc
      481 cggaagtgcg ctggtccaca attatggaag atctgcgccg ccgggatttt tctctgaacg
      541 ccgtggccat ttcgcttaat ccggcgtcgc ggggattatt gctcgatccc actaatggcc
      601 tgtcggatat tgaacgcgcg gaagttcgtg cgttgaccat tcatgcattt acgaatcaac
      661 cggtgcgact gctgcgtgtg ttgcgctttg ctgcgcgtat ggggttcaaa attgaagcgc
      721 ggacgcagga atggtttgat ctggcggtcg agagggaatt gccggccacc attactccgg
      781 aagatgctgg cgccgaactt cgagccttgg ctcgcgaaga gcgccctgcc gtggtgttaa
      841 aagcctggga agatcacgac ctactcgaag cggtcaaccc cttgctggcg aagaagcatc
      901 ctgggtatga cgcgattaac cggctatcaa atgtccgtga ggatctgttt accgctggat
      961 tgcggccgcg tctcgcgacc cccatgcttc tagccatcct tgcgcgactt aaagaccgag
     1021 agcagagcgg cgtactttcc aaggcgggct accggtctgc cgaatctcag gaagtgctga
     1081 actttgacaa gcatattcaa actgtccaaa aagaattaac cggcaaaaaa gttggcactc
     1141 cgctggaggc gtataaattc ctggagaaac ttcctgtcga ccagatcgct tacattatgg
     1201 cggaatcgaa taattcaggg gcggtctcca agattcgcgc gtatttgaac aagtggcggc
     1261 cgctccgttc cgggctacct
//
LOCUS       DATCBB010000808         1596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_446783, whole genome shotgun sequence.
ACCESSION   DATCBB010000808 DATCBB010000000
VERSION     DATCBB010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446783"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VII37_08940"
     CDS             complement(<1..1072)
                     /locus_tag="VII37_08940"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY5069904.1"
                     /translation="MKQPRLLYLGAITLACLAGAGAPANAKLPDTQLKSAAKPGPGKL
                     AGVVLDGTGTPQMGATVELLAEASGVNIARQLLTNTEGAFRGEKLSPGLYTVRVTLAG
                     FLPTLEKHIRINPNVTTVVRVQMESMFASLEQLRRQPVNIPVEGDDWKWVLRSASATR
                     PVLEWMGDAQVMTADASMDRGVPRAPRARLEFTDGARHPGSGSNLVAAPATAFAYDQK
                     LGGTSRVILAGQMSYDQEAPAGSIATVWLPTGSLGAGPHTALVLREAKLGPAGPTFRG
                     VRIDQGGGMAIGDRAILSYGGEFVLVGLGAAASSIRPRTKLDVRVSDDWNATVIFASL
                     PNGPGPLEAAGGEADNMAAALNE"
     gene            complement(1069..>1596)
                     /locus_tag="VII37_08945"
     CDS             complement(1069..>1596)
                     /locus_tag="VII37_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069905.1"
                     /translation="QSGNVGVVNARLQVPRYEDDKLETSSLILADQIEHVPAKQIGNG
                     QFVLGSSKVRPRLEGDFTTADKLGIYMQVYNLKPDDKTHKSNATFQYTVKKGGEQVLQ
                     FKETSVDMKQTGDQITIERLLPLSTLAPGKYTLEINATDTLSNQTISRTADFTVKHPL
                     ETKSAANVQPGGTAK"
BASE COUNT          320 a          526 c          391 g          359 t
ORIGIN      
        1 actcgttcaa cgccgcggcc atgttatcgg cctcgccccc agcagcttcc aaaggccccg
       61 gcccattcgg tagggaagcg aaaattacgg tcgcgttcca atcatccgaa acgcgcacat
      121 ccaatttcgt tcgcgggcgt atggacgacg ccgcagcacc aaggcccacc aaaacaaatt
      181 ccccgccata gctcagaatc gcgcgatcac caatcgccat cccgccgcct tgatcaatgc
      241 gcacgccgcg aaatgtaggc ccagctggtc caagcttggc ttcacgcaaa accaacgcgg
      301 tatgcgggcc cgcgcctaaa gacccggtag gcagccaaac tgtcgcaata cttcccgcag
      361 gcgcctcctg gtcataactc atctgtccgg caagaatcac ccggctggtt ccgcccagtt
      421 tctggtcata ggcaaacgcc gttgccggag ccgccaccag gttcgaacct gaaccgggat
      481 gccgcgctcc atctgtaaat tcaagccttg cgcgcggagc tcgaggcact ccacggtcca
      541 tacttgcatc cgccgtcatc acctgcgcgt cgcccatcca ctccagtacc ggccgtgtcg
      601 cagacgccga ccgcagcacc catttccaat cgtcgccttc caccggaata tttaccggct
      661 gccgccgcag ttgctccagc gacgcaaaca tcgactccat ctgcacgcgc accacagtgg
      721 tgacgtttgg attgatgcga atatgttttt ccaacgtggg taaaaatccc gcaagcgtaa
      781 ctcgtaccgt gtacaagccc ggcgaaagtt tctcgcctcg gaaagcacct tctgtgttgg
      841 tcagtagttg ccgcgctata tttacaccgg aagcttccgc gagcagctct acagtcgcgc
      901 ccatttgcgg cgtccccgtc ccatccagaa ctactccagc cagtttgcct gggcccggtt
      961 ttgcagcact tttcaactgg gtgtccggta acttcgcatt agccggcgca cctgcgcctg
     1021 ccaagcacgc cagcgttatg gccccgagat agaggagcct cggttgtttc actttgcggt
     1081 acctccaggt tgtacgttcg ccgcgctctt ggtttccaat ggatgtttca ctgtgaaatc
     1141 tgccgtccgc gaaatcgtct gattcgaaag cgtgtccgta gcgttgatct ccagcgtata
     1201 cttgcccggc gcgagcgtcg acaacggcaa cagccgctcg atcgtaatct gatcgcccgt
     1261 ttgtttcata tccactgacg tttccttgaa ttgcaaaacc tgctcgccgc cctttttcac
     1321 cgtgtactga aacgttgcgt tggacttatg ggttttgtca tccggcttga ggttgtacac
     1381 ctgcatgtaa attcccagct tgtctgcagt agtaaagtca ccttccagtc gcggcctcac
     1441 cttggaagag cccagcacga actgcccgtt tccaatttgc ttggcgggca cgtgttcaat
     1501 ctgatctgcg agaatcaacg aactggtctc cagtttgtcg tcctcgtaac gcggcacctg
     1561 cagccgtgca ttcactacgc caacgttacc gctctg
//
LOCUS       DATCBB010000809         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_317424, whole genome shotgun sequence.
ACCESSION   DATCBB010000809 DATCBB010000000
VERSION     DATCBB010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317424"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VII37_08950"
     CDS             <1..419
                     /locus_tag="VII37_08950"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5069906.1"
                     /translation="DVWARNAGPDRTSRGWSWKSIADAGGRYAFGSDWPVVTLNPWEG
                     LQTAVTRQTQEGTPPGGFVPSQRLTVAQAVDGYTLGAAFAGRREKTEGSLEKGKLADL
                     IIVSQNIFEIDPHKISETKVRTTIVGGKVVYQSDGK"
     gene            493..1533
                     /locus_tag="VII37_08955"
     CDS             493..1533
                     /locus_tag="VII37_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5069907.1"
                     /translation="MALLLFALFCAACKPKQPTLTLLVWEGYADPSFIAAFEESHRCK
                     VVASYMGTSDELVAKLRGGSASNYDVISPSSDVAASIVRAGLAAPLDLSKIPAYSQLS
                     AKLRDSNIVKLNNQVYGVPFVWGPNPLLYDTTAFAHAPESWAVLWDAKYRGKVSVWDE
                     LSTIYMAAQVLGYDKPDPSQLYNLSDAQLEAVKKKLIELKPNIRKMWATGGELTSLFQ
                     NHEVVIAMGWPLMTNDLRKLNFPIGETIPRENTTGWIDHLMITAASSNKELAQAFLAY
                     MVEAQTQKKVSDVTHYTPANPGAAQFLSADEKKMLHLDDADAYMQRIYFWQDVPRRPK
                     YNEIWNEVKAAQ"
     gene            1530..>2542
                     /locus_tag="VII37_08960"
     CDS             1530..>2542
                     /locus_tag="VII37_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5069908.1"
                     /translation="MNPAPAIPPSEADRAAGPLLEVRALAKRFGAHEVLKTISLNIAA
                     GEFITLLGESGSGKTTMLRLIAGFEQPSSGEIWMKGVRLDTLPPYKRRVNTVFQQYAL
                     FPHLNVRDNVAYGLRVTNAPANEIGKRVDDALRMVKMDSFAGARPATLSGGQQQRVAL
                     ARALVNRPLLLLLDEPLSALDANLRKEMQIELKSLQREVGITFLFVTHDQDEAMALSD
                     RIALLRNGALEQIASPREIYSHPATTYTAQFIGQTNLLRAEVRGGTASCGALHWPTPA
                     QDGTAVFSLRPEAIRLANGVVHAAANSPATDVRFSAIVKQQLYGGASELLEVDCGSQI
                     LRV"
BASE COUNT          570 a          728 c          713 g          531 t
ORIGIN      
        1 tggatgtttg ggcgcgcaat gctgggccgg atcgcacgtc tcgcggatgg tcgtggaaaa
       61 gtattgccga tgcagggggg cgctacgcct tcgggagcga ctggccggta gttaccttga
      121 atccgtggga gggtttgcaa accgccgtga cgcgccagac tcaagaaggc acgccgccag
      181 gtggttttgt accgtcacag cggctgaccg tcgcgcaagc cgtggatggt tatacactcg
      241 gcgccgcctt tgcaggccgc cgcgaaaaaa cggaagggtc gctggaaaaa ggcaagctcg
      301 cggacctgat catcgtttcg cagaatattt ttgaaatcga tccgcacaaa atttccgaaa
      361 ccaaagttcg taccaccatc gttggtggca aggtggttta ccagtccgat ggcaaataat
      421 ttcaaaacga ttttgcgtgg cacaaacttt tcgaccagcg gaggcagtca aatagtgggg
      481 cgatgcggtg tcgtggcgtt gctgcttttc gctctctttt gcgccgcttg taaacccaag
      541 cagcccacgc tcacgctgct ggtgtgggag ggctatgccg atccgtcgtt catcgccgcg
      601 ttcgaggaaa gccatcgctg caaagtggtg gcttcctata tgggcacgag cgatgagttg
      661 gtcgccaagt tgcgcggcgg cagcgcctcc aactatgacg tgatctcgcc ttcgagtgat
      721 gtcgccgctt ccatcgtgcg cgcggggctt gccgcgccgc tggacctctc taaaattcct
      781 gcctactcgc aactttccgc gaagctgcgc gattcaaaca tcgtcaagct caacaatcaa
      841 gtgtacggcg tgccatttgt gtggggacca aacccgctgc tgtacgacac caccgcgttc
      901 gcgcacgctc cagaatcgtg ggcggttttg tgggacgcaa aatatcgcgg caaagtctcg
      961 gtgtgggatg agctttccac catctacatg gccgcgcagg tacttggcta tgacaaaccc
     1021 gatccgagcc aactttacaa tctgagcgat gcgcaactcg aagcggtgaa gaaaaagctc
     1081 atcgagctca aacccaatat tcgcaaaatg tgggccactg gcggcgagct gactagtctt
     1141 ttccagaatc atgaagtggt gatcgccatg ggttggccgc tgatgaccaa cgaccttcgt
     1201 aaattaaatt ttcccattgg cgaaaccatc ccgcgcgaaa acaccaccgg atggatcgat
     1261 cacctgatga ttaccgcggc cagttctaac aaagaactgg cgcaggcttt tcttgcctac
     1321 atggtggaag cgcagacgca gaaaaaagtc agcgacgtta cacactacac gcccgccaat
     1381 cccggcgcag cgcagttttt gagtgccgac gagaagaaaa tgctgcatct cgacgatgcg
     1441 gacgcttaca tgcagcgcat ttatttctgg caggacgttc cgcgccgtcc caaatacaac
     1501 gaaatctgga atgaggtgaa agctgcacag tgaaccctgc gcccgccatt ccgccatcgg
     1561 aagcagaccg ggcagctggg ccattgctgg aagttcgcgc gctggccaaa cgctttggcg
     1621 cacacgaagt cttaaaaaca atttccctga atattgccgc aggcgaattc attactctgc
     1681 tgggggaaag cggctccggc aaaaccacca tgctgcgatt gattgccgga tttgaacaac
     1741 ccagctccgg cgaaatttgg atgaagggcg tacgcctcga cactttgccg ccttacaagc
     1801 gtcgcgtgaa cacagtattt cagcaatacg cgttatttcc ccatttaaat gtgcgcgaca
     1861 acgtggctta cggcttgcgc gtgaccaacg cgcccgcaaa tgaaatcggc aagcgcgttg
     1921 acgacgcgct gcgcatggtg aagatggaca gtttcgccgg cgcgcggccc gccacgctta
     1981 gcggtggcca gcagcagcgc gtcgcactgg cgcgcgctct ggtgaatcgc ccgttacttt
     2041 tgctgctgga cgaaccgctc tccgccctcg acgcaaattt acgcaaagaa atgcaaatcg
     2101 aactgaaatc tctgcaacgt gaagtgggca tcaccttttt gttcgtcacc cacgatcagg
     2161 atgaagccat ggcgctttcc gatcgcatcg cgctgctgcg caacggcgcg ctcgaacaga
     2221 tcgcgtctcc gcgcgaaatt tattcgcatc ccgccacaac gtataccgcg caattcatcg
     2281 gccagaccaa tttgttacgc gccgaagtgc gcggtggaac cgcgagttgc ggcgccttac
     2341 attggcccac acccgcacaa gatggcacgg ccgttttctc gttgcgtccc gaagcgattc
     2401 gacttgcgaa cggcgtggta catgccgccg caaattcacc agcgacggac gtgcggttct
     2461 ctgcgatcgt caagcaacag ctttatggcg gcgccagcga attgctggaa gtagattgcg
     2521 gcagtcagat cctgcgcgtg aa
//
LOCUS       DATCBB010000810         2910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_382125, whole genome shotgun sequence.
ACCESSION   DATCBB010000810 DATCBB010000000
VERSION     DATCBB010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382125"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..776)
                     /gene="ribA"
                     /locus_tag="VII37_08965"
     CDS             complement(138..776)
                     /gene="ribA"
                     /locus_tag="VII37_08965"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY5069909.1"
                     /translation="MDKKLKSTTLEKAKDVELQMVAQAKLPTRYGDFTIYGFKGAGAL
                     DEAVALVRGNLKGKVAPLVRVHSQCLTGDVLASMRCDCRAQLELSLREIGRAPAGVLI
                     YLPQEGRGIGLMNKLRAYELQDGGMDTVQANEKLGFAADSRDYEFCAAILKQLGARRI
                     RLLSNNPEKVRQLENAGIQVVERVPCQPRISKTSRAYLKTKRTKMGHLLKGV"
     gene            1010..2329
                     /locus_tag="VII37_08970"
     CDS             1010..2329
                     /locus_tag="VII37_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5069910.1"
                     /translation="MDPTTSTRDLLFTLLLKVGVASSIAALLARWNTFRRVLFTEERD
                     PDQKLKLMLFMTPPLILGVTLRLVGYPYRFADLSLEGAFLMGLLGGRVVGPIGGAIIT
                     MPALIGHEWLGMPAAATAGLIGGLIRQAIPNKEDIWNFGPFTFLNIPRAIVRLLRRAQ
                     PSWEMAPLLACVGLELGRVALVHATKPKWLFAIDPQWNWWLILTVIATLMSVAAPLKI
                     WNNTRIEMNLERHQQLLLKARMDALSSQINPHFLFNTLNTVSALIRFDPDTARGVVLK
                     LSNILRRLLRKHETFVPLHEELEFIDDYLDIEVARFGRDNLEIIKELDEDTLEAFVPS
                     MLLQPIVENCLKHGLAPKIEGGKIQLRTSNRHGRLHIEIEDNGVGISEEKMPHVYVEG
                     IGLSNVRERLKVLYGADFNLEIESRPGDGTTIRIDIPELVSTMQAVG"
     gene            2470..>2910
                     /locus_tag="VII37_08975"
     CDS             2470..>2910
                     /locus_tag="VII37_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5069911.1"
                     /translation="MTEHTQSPPLSPAAQKFVLHWGEMGTRWGINRTVAQIHALLFVS
                     PHPLPADEIAVILAVARSNVSTSLRELQGWRIVRVVHVLGDRRDHFDCTKDVFEIFRI
                     VSEERKRREIDPTLKLLAECVQEVKAHPEKDAYTLARLESMLDFL"
BASE COUNT          637 a         1039 c          617 g          617 t
ORIGIN      
        1 aaattattcg gatgagaatc tggcagccgt ttctaaacga tacgtcacac gagacttttg
       61 tttcttttca agttggatca cgcacctcaa ggttcgtcga ttttgctaaa cggtcaacga
      121 actgtgcgtt tgactgttca cacgcccttc aacaaatgcc ccatcttcgt tctcttggtc
      181 ttcaaataag cccgcgaggt ctttgaaatt cgcggctggc acggcacccg ttccaccacc
      241 tgaatgcccg cgttctcaag ctgccgcacc ttctccggat tatttgaaag caaacgaatt
      301 cgccgtgctc ccagctgctt caaaatcgcc gcgcaaaatt cataatcgcg tgagtccgcc
      361 gcaaatccca gcttctcgtt agcctgcacc gtatccatgc caccatcttg cagttcatac
      421 gcgcgcagct tattcatcag cccgatcccg cgcccctcct gcggcaaata aatcagcacg
      481 cccgccggcg cacgcccaat ctctctcagc gatagctcca actgggcccg gcaatcgcat
      541 cgcatggaag ccagcacatc tcccgtcaag cattgcgaat gcacccgcac caacggcgca
      601 accttgccct tcaaattccc gcgcaccaac gccaccgctt catccaatgc acctgcgccc
      661 ttgaatccat agatcgtgaa gtccccatag cgcgtaggca gtttcgcctg cgccaccatc
      721 tgcaactcca catccttcgc cttttccaaa gttgtactct tcaatttttt atccatggtt
      781 gaaatcgcaa actatagaag ataaccaacg gcgcgttaca cctattcctg ggaacttacc
      841 taccgcgccc ttcgctgctt aacgaagcgc tctcttaacc ctcgcctccg aatcgcttcc
      901 ataactcgaa tccgcgtctc gcattagatg cccgcataca aattcccgct acacacttta
      961 catcccgcgt aacattagct tagcgtaagc aaagtcagct caacgcatca tggacccaac
     1021 cacctcaacc cgcgatctgc tcttcacctt attgctcaag gtaggcgtcg cgtcctccat
     1081 tgccgcactt ctcgcgcgct ggaacacttt ccgccgcgtc ctcttcaccg aagagcgcga
     1141 tcccgaccaa aaattaaaac tcatgctgtt catgacgccg ccactcatcc tcggcgtcac
     1201 cctccgtctg gtaggctacc cgtaccgctt cgccgatctc tccctcgaag gcgctttctt
     1261 aatgggcctg ctcggaggcc gcgtcgtcgg ccccatcggc ggcgccatca tcaccatgcc
     1321 agcgctcatc ggtcacgagt ggctaggcat gcccgcagcc gccaccgcag gcctgatcgg
     1381 cggcctcatc cgccaagcta tccccaacaa agaagacatc tggaattttg gcccttttac
     1441 ttttctaaac atcccccgcg ccatcgtccg cctgctgcgt cgcgcgcagc cttcgtggga
     1501 aatggccccg cttctcgcgt gcgttggcct cgaacttggt cgcgtcgcgc tcgttcacgc
     1561 caccaagccc aaatggctct ttgccatcga cccgcagtgg aactggtggc tgattcttac
     1621 agtcatcgcc acgctcatgt ccgtcgccgc gccactgaag atctggaaca acacccgcat
     1681 cgaaatgaat ctcgagcgcc accagcagct tctgctcaag gcccgcatgg acgcgctctc
     1741 cagccagatc aatccgcatt tcctcttcaa cactctcaac accgtctccg ccctcatccg
     1801 cttcgatccc gacaccgctc gcggcgtagt cctcaagctc agcaatattc tgcgccgcct
     1861 gctgcgcaag cacgaaacct ttgtcccgct ccacgaagaa ctcgaattca tcgacgacta
     1921 tctcgacatc gaagtagccc gcttcggccg cgacaatctc gaaatcatca aggagctcga
     1981 cgaagacact ctcgaagcct tcgtccccag catgctcctg caacccatcg tggaaaattg
     2041 tctcaagcac ggcctggccc ccaaaattga aggcggcaaa attcagttgc gcacctccaa
     2101 ccgtcacggc cgcctgcaca tcgaaatcga agacaacggc gtcggcatct ccgaagaaaa
     2161 aatgccccac gtctacgtcg aaggcatcgg cctcagcaac gtccgcgagc gcctgaaagt
     2221 tttgtacggc gccgacttca acctcgaaat cgaaagccgc cccggcgacg gcaccaccat
     2281 ccgcatcgat atccccgaac tagtctccac catgcaagcc gtcggctaac cgtttcgcgc
     2341 ttcgccggaa actcaccatc agggcaggga acttccgccc caacccaagc tgatccgtgc
     2401 gatttaacgg cagcctaatt ttgctcttga catcctcccg tcactactgt actttctgtt
     2461 tatacagaaa tgactgaaca tacacaaagt cctccgctct cccccgccgc tcaaaaattc
     2521 gtcctccact ggggcgaaat gggcacccgc tggggcatca accgcaccgt ggcgcaaatc
     2581 cacgctctgc tcttcgtctc gccgcacccg ctacccgccg acgagatcgc cgtcatcctc
     2641 gccgtcgctc gctccaacgt cagcaccagc ctccgcgaac tccagggctg gcgcatcgtt
     2701 cgcgtcgtcc acgtgctagg cgatcgccgc gaccatttcg attgcaccaa agacgtcttc
     2761 gaaatctttc gcatcgtctc cgaggaacgc aagcgccgcg aaatcgatcc caccctaaag
     2821 ctgttagccg aatgcgtcca ggaagtgaaa gcccatccgg aaaaagacgc ttacaccctc
     2881 gcgcgcctcg aaagcatgct cgattttcta
//
LOCUS       DATCBB010000811         1793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_156588, whole genome shotgun sequence.
ACCESSION   DATCBB010000811 DATCBB010000000
VERSION     DATCBB010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156588"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VII37_08980"
     CDS             complement(<1..1470)
                     /locus_tag="VII37_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5069912.1"
                     /translation="MQQSRTHVRLLIVAGVALLWATAVFGRLGYLQLYRHREYLLRAQ
                     HQQQRIIEISPKRGAIYDRNMHALAMSIKVDSAYAIPNEIIDKALAAQLLSGVLGMPA
                     DLVESRLNSSQNFVWIERKLTAEKSEAITALNLKGVYTQAENKRFYPKADLASHVVGF
                     VDIDEKGLGGIEYALDNQIRGKSEKIVMMTDAHQKWFDGGEARRDRGANVVLTLDEKI
                     QYIAERELAAAINKTHALAGSVVVMNPNNGELLAVANWPTFDPNAASAGIPAESRMNR
                     AVSALYEPGSTFKLITLAAAFDQGITNADEVFDCENGAIYVAGHKIHDHKRFGLLTVA
                     DILALSSDVGAIKIAERLGAPKFYDYIHAFGFGTPTGVDLPGESRGLLRPLANWSPIS
                     IGAISMGQEVGVTPMQLISAVSAIANGGMLYKPHIVAEVRRGETVLPREGLLAANEPR
                     RVIQPETAATLRRLMEGVVLNGTGTRARLDGWTSAGKTGS"
     gene            complement(1596..>1793)
                     /locus_tag="VII37_08985"
     CDS             complement(1596..>1793)
                     /locus_tag="VII37_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069913.1"
                     /translation="RLEASALNSSLRLEIAGLSNPKRIDMIARRQLGLTETLPSQVRE
                     YQPVAGAEVADAQLIRVNHEQ"
BASE COUNT          380 a          501 c          504 g          408 t
ORIGIN      
        1 ggacccggtt ttacccgcag aggtccagcc atccagtcgc gcgcgtgtcc cagtgccatt
       61 cagaaccacg ccttccatca gccggcggag cgttgccgcg gtctccggtt gaatgacgcg
      121 acgtggctcg ttagcggcca gcaagccttc acgaggcaat accgtttcgc cgcgacgaac
      181 ttcagccacg atgtgcggct tgtaaagcat gccgccgttg gctatcgcgg ataccgcgga
      241 gatcaactgc atgggtgtta cgcctacttc ctgccccatg gagattgctc caatggaaat
      301 gggcgaccag ttcgcaagcg gccgtaacag tccgcggctt tcgccaggca ggtcgacccc
      361 tgtgggcgtg ccaaatccga aggcgtgaat gtaatcgtaa aattttggcg ccccgaggcg
      421 ctcggcaatt ttgatggcgc ccacatcgct cgatagcgcg agaatgtctg caactgtcag
      481 taggccaaaa cgtttatggt cgtgaatttt gtggcccgcc acataaatgg cgccattttc
      541 gcaatcaaaa acttcgtcgg cgttggtgat gccctgatca aacgccgccg ccagcgtaat
      601 caatttaaaa gtagagcccg gctcatacaa tgcgctgacg gcgcgattca tgcgagattc
      661 cgcagggatt cccgcggacg cagcgttggg atcgaaggtc ggccagttag caaccgccag
      721 caattccccg ttgttggggt tcatcaccac cacggagccg gccagcgcgt gcgttttgtt
      781 tatggccgcg gccagctcac gttccgcgat gtattgaatt ttttcgtcca gcgtcagtac
      841 tacgttggca ccgcgatcgc ggcgagcttc tccgccatcg aaccatttct ggtgcgcatc
      901 cgtcatcatg acgatctttt cgcttttgcc gcgaatctga ttgtcgagcg cgtactcgat
      961 accgcccaac cccttttcat cgatatccac gaaaccaacc acgtgagatg ccagatccgc
     1021 tttgggatag aaacgcttgt tttccgcctg ggtataaaca cctttcagat tcagagcggt
     1081 aatggcctcg gatttttcag cggtgagctt gcgttcgatc cacacaaaat tttgcgaaga
     1141 attcaggcgt gactccacga ggtctgcggg catgccaagc acgccggata acagctgggc
     1201 agcgagcgcc ttatctatga tttcattggg tatggcatac gccgagtcaa ccttgatgct
     1261 catggccagc gcgtgcatgt tgcgatcgta aatggcgcca cgcttgggag aaatttcaat
     1321 gatgcgttgc tgttggtgct gtgcgcgaag taggtactcg cggtgccggt acagttgcag
     1381 gtagcccaac cggccaaaaa ctgcggtggc ccacaagaga gccacgccag cgacgatcag
     1441 caaacggacg tgagttcgag attgttgcat gtaagaatta cgccggtacg ccctcccgtg
     1501 tgacgctcca cttaaaagag aaattgttgt caatgactga gaaggagagg aaggctttgg
     1561 agactctggg cgcccgggtc ctcatgtggt ctggactact gctcgtggtt cacgcgaatt
     1621 aactgcgcat ccgccacttc ggctcccgcc acaggttggt attcacgcac ttgagagggc
     1681 aacgtttccg tcaagcccaa ttggcggcgc gcaatcatgt cgatgcgttt aggattgctg
     1741 agacctgcta tttcgagtcg taaggatgaa tttaaagccg atgcttccag gcg
//
LOCUS       DATCBB010000812         1877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_26139, whole genome shotgun sequence.
ACCESSION   DATCBB010000812 DATCBB010000000
VERSION     DATCBB010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26139"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..656
                     /locus_tag="VII37_08990"
     CDS             156..656
                     /locus_tag="VII37_08990"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5069914.1"
                     /translation="MDYVWSPWRYNYIAHANDEKQPECIFCDALAQKDDAVSLIVYRG
                     KKVFVILNRYPYTSGHVMIVPYAHVAQLALCDAETLAEMMQLAQRVEKSFVANYKPDG
                     MNIGMNLGKAAGAGVAGHLHLHALPRWFGDTNFMTVTGETRVHPEDLQKTFERMRKAL
                     GESSTA"
     gene            674..1822
                     /locus_tag="VII37_08995"
     CDS             674..1822
                     /locus_tag="VII37_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5069915.1"
                     /translation="MDLQLTEEQQLLQKSVREFAESEVRPFAKELDETGHFPREIFKK
                     AAELGLTGIALPENEGGAGFDHVAYTIVIEEISRCCASTGVILSVQNSLYCDPIHRFG
                     TAEQKEKFLLPFARGEKIGCYALTEPQAGSNAAALQTKAVRNGDSYVVNGTKAWITNG
                     GAADAAIVYVNTDPAKGEKGITALVVEKGAPGFRVGKEEKKLGISATACSELVFSDCN
                     VPAANRIGNEGEGYKVALSTLDGGRIGIAAQATGIAQGAFEAALKYSQERMAFGNPIS
                     QFQAIQFMLADMATEIDAARLLIRKAAWKQDSGARFSQEAAVAKLFASEMATRVTHKA
                     IQIHGGNGYSREYPVERNYRDARITEIYEGTSEIQRLVIASWVLKAGA"
BASE COUNT          447 a          447 c          587 g          396 t
ORIGIN      
        1 ggctacgcct gccagttcgg cgcccggcga cgcgactcgt tcctgaagca ctgcttctgg
       61 atccatttcc taaaaagaca tctggtagtg acgcgcgcgg cgtaattgca ttgtgcgtta
      121 tgctgtgcga gcgtcgtttt ctttgaggat gcctgatgga ttatgtgtgg tcgccgtggc
      181 gttacaacta catcgcacac gcgaatgacg agaagcagcc ggagtgtatt ttctgcgacg
      241 ctttggcaca aaaggatgat gccgtgtcgc tgatagtcta tcgcggcaaa aaagtgttcg
      301 tgatcctgaa tcgctatccc tatacatccg ggcacgtgat gatcgtgccc tacgcgcacg
      361 tcgcgcaatt agcattgtgc gacgcggaga ctcttgcgga gatgatgcag ttggcccagc
      421 gggtggagaa gtcctttgtt gcgaactaca aacccgatgg gatgaatata ggcatgaacc
      481 tcgggaaagc tgccggcgcg ggtgttgccg gacacctgca cctgcatgcg ctgccgcgat
      541 ggtttgggga taccaacttt atgactgtaa cgggcgaaac gcgagtgcac ccggaagatt
      601 tgcagaagac ttttgagcgc atgcgaaaag ctttgggtga gagttcaacg gcttaacgcg
      661 gagagcagag cgagtggatc ttcaattgac tgaggagcag cagctcctgc aaaaaagcgt
      721 ccgggaattc gcggaatcag aagtgcggcc gttcgcgaaa gagttggacg agaccggaca
      781 ttttccgcgg gaaatattca agaaagcggc ggaattgggg ctgaccggaa tcgcactgcc
      841 ggaaaatgaa ggcggcgcgg gtttcgatca cgtcgcttac accatcgtga tcgaggaaat
      901 ttctcggtgt tgtgcgtcca ccggcgtgat tttgtcggtg caaaattcgc tgtactgcga
      961 tccgattcat cgctttggta ctgccgagca gaaggagaaa tttcttctgc ccttcgcgcg
     1021 cggggaaaag atcggctgct atgcgttgac agagccgcag gccggttcca atgccgcggc
     1081 cttgcaaacg aaagcggtgc gcaacggcga cagctacgtc gtcaacggca ccaaagcatg
     1141 gatcaccaat ggtggcgccg ccgatgccgc cattgtgtat gtgaataccg atccggccaa
     1201 gggagagaaa ggcatcaccg cgctggtcgt tgaaaaaggg gcgccgggat ttcgggtagg
     1261 caaggaagag aaaaagttag ggatcagtgc cacggcctgc agcgagctgg tcttttccga
     1321 ttgcaatgta cctgcggcaa accgcatcgg caacgaaggc gaagggtaca aagtagcgtt
     1381 atcgacattg gacggcggaa gaattggcat tgccgcgcag gcgacaggaa ttgcccaggg
     1441 cgcatttgaa gcggctttga aatattcgca ggaacgcatg gcgtttggga atccgatttc
     1501 gcaatttcag gccatacaat tcatgttggc ggatatggcc acggagattg acgccgcgcg
     1561 gctattgatc cgcaaagcgg cgtggaagca ggacagcggg gctagattca gccaggaggc
     1621 ggcggtagct aaattatttg ccagcgaaat ggctacgcgc gtgacgcata aggccattca
     1681 gattcatggc ggcaacggtt acagccgcga atacccggtg gagcggaact atcgtgacgc
     1741 gcgtatcacg gagatttatg aaggcacgag cgagattcag cgattagtga ttgcctcgtg
     1801 ggtgttgaag gctggagcct agaagcgcaa aaatgtcagc gcgccgacat ttaaagtaat
     1861 actttactta ttcagta
//
LOCUS       DATCBB010000813         1517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_58539, whole genome shotgun sequence.
ACCESSION   DATCBB010000813 DATCBB010000000
VERSION     DATCBB010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58539"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..498)
                     /gene="queD"
                     /locus_tag="VII37_09000"
     CDS             complement(109..498)
                     /gene="queD"
                     /locus_tag="VII37_09000"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY5069916.1"
                     /translation="MFEVSVEETFSAGHALRGYRGKCENVHGHNYRVRVTLEGPQLDD
                     IGLLVDFTELKRVVREIMGRLDHQFINDLEPFKTVNPSAENLAKYFYDEVTRLLRGLP
                     PGARVTDVILWETDTSRAQYRPTGVKP"
     gene            complement(538..>1517)
                     /locus_tag="VII37_09005"
     CDS             complement(538..>1517)
                     /locus_tag="VII37_09005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5069917.1"
                     /translation="YDSLLTALKGLETEIAAMVQKPEELLRIARRSFELRQEVAFLFE
                     SNEKNYVYWYERRNKGVFLTATPIDVSQILRQRLFEQFDTVVLTSATLTVGGRFEFIR
                     QRLGLDHVKERTLPPEFDYAEQALLYLPTKMPDVRDAGFGSKAADEIVQLLEHSQGRA
                     FCLFTSYSQMNDLFERVRTRVSFPLLLQGTAPRSVLLERFKNTPAAVLFATSSFWQGV
                     DVPGEQLSLVIVDRLPFAVPSDPIVAARVRTLQDDGRNPFSEFQVPQAVLALKQGFGR
                     LIRTKTDRGVLALLDTRIQRMPYGKIFLESLPNYRITHNSADAARFLEK"
BASE COUNT          362 a          425 c          414 g          316 t
ORIGIN      
        1 ccacaatcag cctgggataa cccggaggaa aacccatgtg ccgcagcacc agcacgtaca
       61 tcagcgtgac cagcaacacc ggaatcatga tgatggtata cgagcgattc atggtttcac
      121 gcctgtggga cggtactgcg cccggctcgt atccgtctcc cacaaaatca catcagtcac
      181 acgcgcgccc ggcggtaagc cacgcagcag gcgcgtgacc tcgtcgtaaa aatatttagc
      241 caaattttcc gccgatgggt tgaccgtttt gaacggctca agatcgttga taaattgatg
      301 atcgagccgg cccataattt cgcgaacgac gcgctttaat tccgtgaaat ccaccagcaa
      361 accaatgtca tccagttgcg gtccttcgag cgtgacgcgc acgcggtaat tatgcccgtg
      421 cacgttttcg cacttgcccc ggtatccgcg cagcgcgtga ccggcggaaa atgtttcttc
      481 gacgctgact tcaaacatgg atcctcaaat taagccggga aggattctgc caatctctta
      541 tttttcgagg aagcgcgcag catcagccga attatgcgta atgcggtagt tgggcagcga
      601 ttccaggaaa attttaccat agggcatgcg ttggatgcgg gtgtccagca aggccagcac
      661 gccgcgatcg gtcttggttc gaatcaagcg gccgaatcct tgtttcaagg ccagcactgc
      721 ttgcggcacc tgaaattccg agaagggatt acggccgtcg tcctgcaaag tccgcacacg
      781 cgcagcgacg ataggatcgc tgggaacggc aaagggcaag cggtccacga tcaccaggga
      841 aagttgctcg ccgggaacgt ccacaccttg ccaaaagctc gaggtggcaa atagcacagc
      901 ggcaggtgtg ttcttaaagc gctctaataa caccgaccgc ggcgcggtgc cttgcagcag
      961 tagcggaaag ctgacgcgcg tgcgcacacg ttcgaacaaa tcgttcatct ggctgtaact
     1021 ggtgaagaga caaaacgcgc gaccctggct gtgctccagc aattgcacaa tttcatccgc
     1081 cgctttggaa ccaaaaccgg catcgcggac gtccggcatt ttggtcggta aatacagcag
     1141 agcttgttcg gcgtaatcga attcaggtgg cagggtgcgc tctttgacgt gatcgaggcc
     1201 caggcgctgg cgaatgaatt cgaatcgtcc acccaccgta agcgtcgcgg aagttaacac
     1261 cacggtatcg aattgctcga agagccgttg gcgcagaatc tggctgacat ctataggcgt
     1321 tgcggttaga aagacgcctt tgttgcggcg ctcgtaccaa tacacgtaat tcttttcatt
     1381 ggactcaaac aggaacgcca cttcctgacg cagctcgaaa ctgcgcctgg cgatgcgcaa
     1441 caattcctcg ggcttctgca ccatggcggc gatttccgtc tccaggccct tcagcgctgt
     1501 caaaagagag tcgtaag
//
LOCUS       DATCBB010000814         1894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_26220, whole genome shotgun sequence.
ACCESSION   DATCBB010000814 DATCBB010000000
VERSION     DATCBB010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26220"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VII37_09010"
     CDS             <1..894
                     /locus_tag="VII37_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HEY5069918.1"
                     /translation="NGAAPGTLQTRYWKDFHPAQINGQPGAEPVPEVATASGPQALAL
                     RVYVGPKDYDDLKHMNPPLHGLVNFGWMEFIADPLFHALKWLHNYIPNWGWAIVILTL
                     VINMALFPLRISSYKTTMKMQRVGPEIKAIQERYKKYKLNDPRKAEMNKEVMAVYSRE
                     GINPAGGCFQMFLQMPIWFGLNNALRFAIEMRHARWFGWITDLSARDPYYILPIAMGL
                     SMYLVSKMTPMTSTDPQQQMMMKIMPITMAGIFIISPISSGLAVYILTSSIVGIGQQW
                     YLNRHHPVIAAPVTKPVRGKK"
     gene            943..1479
                     /locus_tag="VII37_09015"
     CDS             943..1479
                     /locus_tag="VII37_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY5069919.1"
                     /translation="MIPNFIHDGKLNRPATAAALQKFLDDSVRAAGLELKVNVRAVPP
                     ETAGLEGHAEVIADLDGRDKEILLERNAEVLKAIEHLAFRALRLEPAFHEKIHIDCAG
                     YRALRFEELRMTAKVAAERVEASRQPFRLNPMSSRERRIVHLALKDIPGVRTESIGMG
                     EERQVVIHPADKSAKAPA"
     gene            1575..>1894
                     /locus_tag="VII37_09020"
     CDS             1575..>1894
                     /locus_tag="VII37_09020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY5069920.1"
                     /translation="MAKRKTKKTRISVKRKSSRPIKSVKLPKDAVALVVAMHAKPGQE
                     LLLQAELTALVRPTRAEQGCLLYDLHRSTDVPTDFLFYEIWSSREAHAAHKQTPHFLR
                     WNAR"
BASE COUNT          460 a          642 c          430 g          362 t
ORIGIN      
        1 aacggcgccg cccccggcac cctgcaaacc cgctactgga aagattttca tcccgcgcag
       61 atcaatggcc aacccggcgc cgaacccgtc cccgaagtcg ccaccgcgag cggcccgcaa
      121 gccctcgcct tacgcgtata cgtcggcccc aaagattacg acgacctgaa gcatatgaat
      181 ccgccgctcc acggcctcgt aaatttcggc tggatggaat tcatcgctga cccgcttttc
      241 cacgccctaa agtggctgca caactacatt cccaactggg gctgggccat cgttattctc
      301 accctggtca tcaatatggc gctgttccca ctgcgcattt ccagctacaa aaccaccatg
      361 aagatgcagc gcgttggccc ggaaatcaaa gccatccagg agcgctacaa aaaatacaag
      421 ctcaacgacc cccgcaaagc ggaaatgaac aaagaagtca tggcggtcta cagccgcgag
      481 ggaatcaatc ccgccggcgg ctgcttccag atgttcctgc agatgcccat ctggttcggc
      541 ttgaacaacg ctctgcgatt cgcaatcgaa atgcgccacg cccgctggtt cggctggatc
      601 accgaccttt ccgcccgcga tccctattac atcttaccca tcgcaatggg tctctccatg
      661 tacctcgtct ccaaaatgac gcccatgacc tccaccgatc cgcagcagca aatgatgatg
      721 aaaatcatgc ccattaccat ggcgggaatt ttcatcattt cgccaatttc cagcggcttg
      781 gcggtgtata ttctcaccag cagcattgtt ggcattggac agcagtggta cctgaaccgt
      841 caccatccgg taatcgctgc cccggttaca aaacctgttc gtggcaaaaa ataataggca
      901 aaaagcaacg ctcgtagtcg caccacttcg ttcgaagggc tgatgattcc gaatttcatt
      961 cacgatggca agctgaaccg tcccgcaacc gccgcagctc tgcagaaatt tctggatgac
     1021 agcgtgcgtg ccgcgggcct cgaattaaaa gtaaatgtcc gcgccgtccc cccggaaacc
     1081 gccgggctgg agggacacgc cgaagtcatc gccgacctcg acggaagaga caaagaaatt
     1141 ctgcttgaac gaaacgccga agtcctgaaa gccatcgagc atctggcttt ccgcgcactc
     1201 cgcctcgagc ccgccttcca cgaaaaaatt cacatcgatt gcgccggcta ccgcgcgttg
     1261 cgtttcgaag agctgcgcat gaccgcaaag gttgccgccg aacgcgtcga agcctcccgt
     1321 caacctttcc gcctcaatcc catgtcctcc cgcgaacgcc gcatcgtcca tctcgcgcta
     1381 aaagacatcc cgggcgtccg caccgaaagc atcggcatgg gcgaagagcg ccaagtcgta
     1441 atccatcccg cagacaaatc cgccaaagcc cccgcctgaa atttgtgctc gctaattaat
     1501 ttggcatcgc ccgcttccaa agaaaaaact gaccgacttc cgaacacttc cgacttggag
     1561 tacaatcccg cgccatggcc aaacgcaaaa caaaaaaaac ccgcatctcc gtcaaacgta
     1621 aatcatctcg ccccatcaaa tccgtcaagc ttcccaaaga cgccgtcgcc ctggtggtcg
     1681 ccatgcacgc caagcccggc caggagctgc tcctccaagc cgaactcact gccctggtcc
     1741 gccccaccag agccgaacaa ggctgtctct tgtacgatct tcatcgttcc accgacgtcc
     1801 ccaccgattt tttattttat gagatctggt ctagccgcga agcccatgcg gcccacaagc
     1861 aaaccccgca ttttttgcgc tggaacgccc gcaa
//
LOCUS       DATCBB010000815         1110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_58559, whole genome shotgun sequence.
ACCESSION   DATCBB010000815 DATCBB010000000
VERSION     DATCBB010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58559"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VII37_09025"
     CDS             complement(<1..710)
                     /locus_tag="VII37_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY5069921.1"
                     /translation="MFSRLPIRALQFTAALVIVIGITLFYRDILAVNQTTVALTFVVA
                     ILAVSTFWGFAVSAFMSVAAMLAFNYFFLPPVGTFTVSDPQNWAALFAFLASSLIASQ
                     LAARVRKEADEAQKRRNEIERLYVFSQRLLVSGNVITLLNAIPNHIVETFEVGAAALY
                     LENKQKFYHSGAGSHFADEELKHATVRDEPLRDPARSLSFVPVRMGTRAIGSLGISGQ
                     LLSRETLDALGSMIAIAF"
     gene            733..>1110
                     /locus_tag="VII37_09030"
     CDS             733..>1110
                     /locus_tag="VII37_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEY5069922.1"
                     /translation="MPKKPEDWLEHAATPEKTKGIFKLFLGYAPGVGKTYSMLSEGIR
                     RHGRGEDVVIGVLETHGRKGIEELAPELEAVPRRSIEYKGTTFEEMDVDAIIARKPAV
                     VLVDELAHTNIPGSRNRKRYEDVQ"
BASE COUNT          278 a          306 c          329 g          197 t
ORIGIN      
        1 tcgaaggcga tggcaatcat cgagcccagg gcgtccagcg tttcccgcga cagcaactgg
       61 cctgaaattc ccaaactccc gatcgctcgt gttcccattc gaacgggaac gaaggataag
      121 ctgcgggccg ggtcgcgcag cggttcgtcg cgcacggtgg cgtgcttcaa ttcttcatcg
      181 gcgaagtgcg accctgctcc ggagtgataa aacttctgct tattctcgag gtacagcgct
      241 gcggcgccca cttcaaatgt ttccacgatg tgattgggga tggcattgag caaggtaatc
      301 acgtttccgg acaccaggag gcgctggctg aaaacgtaca gcctctcgat ctcattgcgg
      361 cgcttttgcg cttcatctgc ctctttgcgg acgcgcgccg caagctggct ggcgatgagc
      421 gatgacgcca ggaaggcgaa tagcgccgcc cagttttgag ggtccgaaac ggtaaatgtt
      481 cccactggcg gcaaaaagaa ataattgaac gccagcatcg cggcaaccga catgaacgcg
      541 gaaacagcga agccccaaaa ggttgaaact gctaaaatcg caacgacaaa cgtcaatgcc
      601 acggtggttt gattgacggc gaggatgtcg cggtaaaaaa gagtaatccc gatgacgatg
      661 accagcgccg cagtgaactg caacgcgcga atcggaaggc gtgagaacac cgtagtattc
      721 tagcaggcgc ccatgcccaa gaagcctgaa gactggctcg agcacgccgc aaccccggaa
      781 aaaaccaagg ggatctttaa gctctttctc ggctacgcgc cgggcgtggg aaaaacctac
      841 agcatgttaa gcgaaggcat tcgccgccac ggccgcggcg aagatgtggt catcggcgtt
      901 ctggagacgc acggccgcaa gggaattgaa gaattggcac ccgaactgga ggccgtgcct
      961 cgacgcagca tcgaatacaa gggcacaact ttcgaagaga tggacgtaga cgcgatcatc
     1021 gcccgtaaac ccgcggtggt gcttgtcgat gagctggcgc acaccaatat tcccggcagc
     1081 aggaatcgga aacgctacga agatgtgcag
//
LOCUS       DATCBB010000816         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_382295, whole genome shotgun sequence.
ACCESSION   DATCBB010000816 DATCBB010000000
VERSION     DATCBB010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382295"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VII37_09035"
     CDS             <1..560
                     /locus_tag="VII37_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY5069923.1"
                     /translation="VFFGDSITDIWKLADSFPGKPYINRGIGGQTTPQMLVRFRADVI
                     DLQPKVLVILAGTNDIAGNTGPMSLPEIEANYASMAELARLHEIHVVFASVLPVNNYT
                     PRAQDFFAQRSPEKILELNRWLKPYCATNGYVYLDYFSAMVDDKGMLKANLAEDGLHP
                     NGAGFKVMAPLAEAAIEKSLETSPK"
     regulatory      591..785
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            848..>1542
                     /locus_tag="VII37_09040"
     CDS             848..>1542
                     /locus_tag="VII37_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5069924.1"
                     /translation="MMTVFLPTTNDRRPTTNYVPLTDKFGRAITDLRISITDRCNYKC
                     VYCRTGNEGALYGDLPFADYLRVARVLAGMGISKVRITGGEPLLRKGVVDFVRELAAL
                     RCTDGEPLDLAITTNGHLLAEMAQPLKEAGLSRVTVSMDAVDPDQFARITRVTNGYER
                     VLAGIRAARDAGLWPVKVNCVLMRGFNEDQIIPFGMFAREEGVVVRFIEFMPLEEERV
                     WAPDTVVPLEEIL"
BASE COUNT          329 a          422 c          455 g          336 t
ORIGIN      
        1 tggtattttt cggagattca attacagata tatggaagct ggctgattct tttccgggaa
       61 aaccctacat caaccgcgga atcggcggac agaccactcc gcagatgctg gtccgcttcc
      121 gcgcagatgt gattgatcta cagcccaaag ttcttgtcat cctggcgggc acgaatgaca
      181 ttgcgggcaa tacgggaccg atgtctttgc cggagatcga ggcgaattat gcatccatgg
      241 cggaactggc tcgactgcac gaaattcatg tagtgttcgc gtccgtgctt ccggtaaata
      301 actacacgcc ccgggcgcag gacttttttg cccaaagatc tcccgagaaa attctggagt
      361 tgaaccggtg gttgaaacct tactgcgcaa cgaatggtta cgtgtatctc gactacttca
      421 gcgcgatggt cgacgacaag ggaatgctca aggcaaatct tgctgaggac ggtttgcatc
      481 ccaacggggc ggggttcaag gtaatggcac ccttggcaga ggcggcgata gaaaagagcc
      541 tggagacgtc gcctaaatag ctccgctgac gccttcctcc tgcatctata atgacattga
      601 gtttccgatc gctccggcct tcgggcatac cctggctgcg gcggcggtgg aaagagttcg
      661 cgggcgagag cctgccctga ggaagtcgaa ggggcgccca cgccacactt tcccaagggt
      721 gctggtggaa acgcctgcgc ctcccggctt cgacaggagc cattggaaag gaaacgctcc
      781 gcgagcatgt gcccgcctgc gtctccctcc gcgttgtttc tacagagcag atctttcgcg
      841 ttcgctcatg atgacagttt ttttgccaac gaccaacgac cgacgaccaa cgaccaacta
      901 cgtgccccta accgacaaat tcggacgcgc catcaccgat ctccggatct ctatcacgga
      961 tcgctgtaac tataagtgcg tctattgccg gactggaaac gaaggcgcgc tttacggtga
     1021 tctgcccttt gccgattatt tgcgggtggc ccgcgtgctt gccgggatgg ggattagcaa
     1081 agtccgtatc accggcggcg agccgctgct gcgcaagggc gtagtggact ttgttcgcga
     1141 actagcggcg ctgcgctgca cggacggaga gccgctcgat cttgccatca ccaccaatgg
     1201 acaccttctg gctgagatgg cgcagccact caaagaagct ggtctcagcc gcgtgactgt
     1261 gagcatggat gcggttgatc ccgaccaatt tgcgcgcatc acgcgcgtaa cgaacggata
     1321 tgaacgcgtg ctggccggaa ttcgcgcggc gcgtgacgct gggctatggc cggtcaaagt
     1381 caactgcgtg ctgatgcgag gcttcaacga ggatcagatc attccgtttg gaatgtttgc
     1441 tcgcgaggaa ggcgttgtcg tgcggttcat tgaattcatg ccgctggagg aagaacgtgt
     1501 ctgggcgccg gatacggtcg tgcccttgga agaaatccta ag
//
LOCUS       DATCBB010000817         1103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_221677, whole genome shotgun sequence.
ACCESSION   DATCBB010000817 DATCBB010000000
VERSION     DATCBB010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221677"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VII37_09045"
     CDS             complement(<1..661)
                     /locus_tag="VII37_09045"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY5069925.1"
                     /translation="MAAPFNILSNAPLTPPAFPVGTHPRPDWLRVKFFGGERYQDLKR
                     IMRTLELHTVCESARCPNMGECWEHGTATFMILGDICTRACGFCAVPSGKPAGPPDEN
                     EPLRVAEAVEKMGLRYAVVTSVNRDDQPDGGAQIFARTIEEIRRRVPGCKVEVLIPDF
                     RGNWDALNLVLATRPDVLNHNTETVPRLYRQVRKGALYERSLELLKRAKTAHPEVPTK
                     TG"
     gene            complement(789..>1103)
                     /locus_tag="VII37_09050"
     CDS             complement(789..>1103)
                     /locus_tag="VII37_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069926.1"
                     /translation="VTKRNGSSVEQKQMTTWHKRDWKQFYELARRPWQRHRPPRPIYP
                     TGINRVLPAAGFSLSELDDAGLDLDLAESLGLPVDAGRIGAYGPNVTTLRDFVRSSRQ
                     PL"
BASE COUNT          215 a          320 c          347 g          221 t
ORIGIN      
        1 atcccgtctt ggtcggcact tctggatgcg cggtcttggc gcgcttcaaa agctccagcg
       61 aacgctcgta gagcgcgccc ttgcgcacct ggcgatacag gcggggaacc gtttccgtgt
      121 tgtgattgag tacgtcggga cgggtagcca gcaccaagtt gagcgcgtcc cagttgccgc
      181 gaaaatcggg gatcagcact tcgactttgc agccggggac gcggcggcga atttcttcga
      241 tagtgcgcgc aaaaatctgg gcgccgccgt cgggctggtc gtcgcgatta acagaagtaa
      301 ccaccgcata ccgcaaaccc attttctcta ccgcttccgc cacgcgcagc ggttcatttt
      361 cgtccggcgg gccggccggt ttgccggaag gcacggcgca gaatccgcag gcccgcgtgc
      421 agatatctcc cagaatcatg aacgttgcgg tgccgtgttc ccagcattcg cccatgttcg
      481 ggcagcgggc gctttcgcac acggtgtgca gttcgagggt gcgcatgatg cgcttcaggt
      541 cctggtagcg ttcgccgcca aaaaatttga cgcgcagcca gtcggggcga ggatgggtac
      601 ccaccggaaa tgccggaggc gtgaggggtg cattcgaaag gatgttaaac ggggcagcca
      661 tcaggttgag ccagaaactt tattttacgc acctgcgcgg caaagcgcca atggcgcgtg
      721 cctggttggc gagcggaagt aaccttcaaa agtaactttc agattgcgtt gtggaatcgg
      781 actaccggtt agagcggctg ccgcgaggag cgcacaaagt cgcgcagagt cgtaacgttt
      841 ggcccgtaag cgccgatgcg cccggcatcc accggcaagc ccaggctttc agccagatcc
      901 aggtccaggc ccgcatcgtc cagttcagag aggctgaatc ccgcggcggg cagaacacga
      961 ttaatccccg tcggatagat cggccgggga ggacggtgac gctgccaggg ccggcgcgcc
     1021 agctcgtaaa actgtttcca atcgcgtttg tgccaggtcg tcatttgttt ctgctcaact
     1081 gaactgccat ttcgtttcgt tac
//
LOCUS       DATCBB010000818         1758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_58733, whole genome shotgun sequence.
ACCESSION   DATCBB010000818 DATCBB010000000
VERSION     DATCBB010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58733"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1205)
                     /locus_tag="VII37_09055"
     CDS             complement(117..1205)
                     /locus_tag="VII37_09055"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEY5069927.1"
                     /translation="MCSGCGKARRRSYGKRWRRVRDLDCGDREVYLDFQIHRVKCPTC
                     GVKNERLAFLSDNTRFTLRFAMKVGALCRAMTIRDVTRHMRMDWHAVKELDKIYMREQ
                     LRRAGPPGPAVIGIDEVSIKKRHIYRIVVSDLEARRAIWFGGDGRSEKDMDMFYAFLG
                     KENAEKIRLAVMDMWLPFRKSTQAHAPQAAILFDKFHILRHLGEALDKIRKAEYGRIT
                     GPQRKFIKGQKYVLLSHKENLTPEGRHSLKLLLKANKRLNKAYLLREQFDQLWGYTSE
                     TWARRFFDNWKAQLRWQRLGPYEKFAAMIEKHWDGIAAYCKPENKVALGFVEGLNNKI
                     RVIQRRAYGLRDEEYLKLKILTSGLPEL"
BASE COUNT          358 a          512 c          464 g          424 t
ORIGIN      
        1 caatacgaca ccggcataaa tatagttcaa gaagccattg aacggggttc attcagactc
       61 cggccctccg taattctggc tcttcgcgga ttttggttgc ggattttggg gtgttttcat
      121 agttcgggca agcctgaggt caggattttc agcttgagat attcctcgtc gcgcaggccg
      181 taggcgcggc gctggatcac gcggattttg ttgttgaggc cctcgacgaa gccgagggcg
      241 actttgtttt ctggcttgca gtacgcggcg atgccgtccc aatgcttctc aatcatggct
      301 gcgaactttt cgtaggggcc aaggcgttgc cagcgcagtt gcgccttcca gttgtcgaag
      361 aaccttcgcg cccaggtttc cgaggtgtag ccccacagct gatcgaactg ctcgcgcagc
      421 aaataggctt tgttcaggcg cttgttggct ttcagcaaaa gcttcagact atgccgccct
      481 tccggcgtca ggttctcttt gtgcgagagc agaacgtatt tttgtccctt gataaatttg
      541 cgctgtggcc ccgtgatgcg tccgtattcc gccttgcgaa tcttgtccag cgcctcgccg
      601 aggtgccgga gaatatggaa cttgtcgaac agaatggcgg cctgcggcgc atgcgcttgc
      661 gtcgatttgc gaaacggcag ccacatgtcc atcaccgcca aacgtatttt ctccgcgttt
      721 tccttgccca aaaacgcgta aaacatgtcc atgtccttct cgctgcgccc gtcgccgccg
      781 aaccagatgg cccgtctggc ttcaagatcg ctgaccacga tccggtagat gtgccgcttc
      841 ttgatggaca cttcgtcgat gccgataacg gcgggcccgg gcggtcccgc gcggcgcagc
      901 tgttcgcgca tgtaaatctt gtcgagttct ttgaccgcat gccagtccat gcgcatgtgc
      961 cgcgtcacgt cccggatcgt catcgcgcgg cataacgcgc cgaccttcat cgcaaaacgc
     1021 aacgtgaacc gcgtattgtc cgacaaaaag gcgagccgtt cgttcttcac gccacacgtc
     1081 gggcatttga cgcggtgtat ttgaaagtcg agatagactt cccgatcccc gcaatcgaga
     1141 tcgcgcaccc gccgccagcg cttgccgtac gaacgacggc gtgctttccc gcatccactg
     1201 cacacccttt ttttgagcgc cggtcgaggg tcagcacgaa ggccggatgt ttcagttcat
     1261 cgtaggcgtc gatcctcccc agcacatgaa aaccaggtat cttgtacgcg tcgcgcagcg
     1321 ttcgacgggg tgaagtgtcg gttggttttt tcatgcccaa aatctggcac gttttttacc
     1381 cgcccaaaag cgatcaaaac ccttgtcttt cctgcctcaa aacaccgtca cacactcgaa
     1441 ttgtgcaacc tttttccgcg atgagccata atttgttaaa ccgtttagcc aatgctcgcg
     1501 ctattgggga accaagaggg accccgatgc accgagtcgg aattgtggaa aatagccgcg
     1561 tgcagcaccg gcggtggccg cctgatcgcc tcgtcaccca gtttcccgca tagctccacc
     1621 agcatcggca aatttaagca tagccctgct attctacaga gagccatcgc ctatctttgc
     1681 aattaattgc aaaggcaaaa atggtctctt gggatgacta aaaaggctca tcgcggaaaa
     1741 aggttgcaca attcgagt
//
LOCUS       DATCBB010000819         1342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_90714, whole genome shotgun sequence.
ACCESSION   DATCBB010000819 DATCBB010000000
VERSION     DATCBB010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90714"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /gene="trmFO"
                     /locus_tag="VII37_09060"
     CDS             <1..1079
                     /gene="trmFO"
                     /locus_tag="VII37_09060"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEY5069928.1"
                     /translation="EPLITIHREEVTQIEEDSLTIIATGPLTSDALSREIVRLSSSPQ
                     LEAEAGADPSHSRAGAPAPHGNLYFYDSISPIVEADSIDMSKVYLAARYDKGSADYIN
                     CPMSKVEYDRFCDALLEAQSVEQKDWENLNYFESCLPIEEIARRGRDTLRFGPMKPVG
                     LKDPRTGRIPYAAVQLRQENLRADSYNLVGFQNHLKFGDQARVLRLIPGLENARFLRY
                     GQIHRNTYINSPALLNSSLQMKANPHVMFAGQICGVEGYVESIATGLIAGMNAAALAS
                     GSELPPPPRATAFGSLIHYITQAESKSFQPTNITFDLLPALDTPVRDRKERHRQQCEL
                     ALRQFDEWYNMHCGQFADPSLRSA"
BASE COUNT          279 a          395 c          379 g          289 t
ORIGIN      
        1 gcgagccgct catcacgatc caccgcgaag aggttacgca gatcgaagaa gatagcctca
       61 cgataatcgc caccggcccg ctcacctcgg acgcgctgtc gcgggaaatt gtgcgcttga
      121 gttcgtcgcc gcaattggag gcagaagctg gggccgatcc ttcgcattcg cgggcggggg
      181 cgcccgcgcc acatggcaat ctttatttct acgattcgat cagcccgatc gttgaggctg
      241 actccatcga catgagcaag gtgtacctgg cggcccgcta tgacaaaggg tcggccgact
      301 acatcaactg tcccatgtcg aaagtggagt atgaccggtt ctgcgacgcc cttctcgagg
      361 cgcaatcagt ggagcagaag gactgggaaa atttgaacta cttcgagagc tgcctgccca
      421 tcgaagagat cgcccggcgc ggacgcgaca cgctgcgctt cggtccgatg aagcccgtcg
      481 ggctgaaaga tccacgcacc ggcaggattc catacgccgc cgtgcaatta cgccaggaga
      541 atttgcgcgc tgattcctac aatcttgtcg gctttcagaa ccatctgaaa tttggcgacc
      601 aggcccgcgt cctgagactg attccaggac tggaaaacgc gcgtttcttg cgctacggac
      661 agattcatcg caacacctat atcaactcac cggcgcttct caactcaagt ttgcagatga
      721 aggcgaatcc gcatgtgatg ttcgcggggc aaatctgtgg agtcgaaggc tacgtcgaat
      781 cgattgcgac cggactgatc gcgggaatga atgcagcggc gctcgcatcc ggcagcgaat
      841 tgccgcctcc gccgcgagcg accgcatttg gatcgcttat ccactacatc acgcaggcag
      901 agagtaagag tttccagccc acgaatatca cattcgatct cttgccagcg ctggataccc
      961 ccgttcgcga ccgcaaagag cgccatcgcc agcagtgcga attggcattg cggcaatttg
     1021 atgagtggta caacatgcac tgtgggcaat ttgcagaccc ctcgctacgc tccgcatgac
     1081 aagttctggg atcgtcgacg cgaagaactc cttgtttttt tcggcgattc gttttagcgt
     1141 gcacttgtcg cagtgaggtg cgtttcaatg ccgatttctg ccgttgattg cgttcagccc
     1201 gccctgcagc atactcgagc ccagctcttc actcatttcc gctggggaca gtggtcccgc
     1261 ttggcgctgg ttggaattct ggccgcggaa ctccatgtcg gcggatgcgg tttcggaaat
     1321 gtcggcggcg cctttcctca cc
//
LOCUS       DATCBB010000820         1073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_252392, whole genome shotgun sequence.
ACCESSION   DATCBB010000820 DATCBB010000000
VERSION     DATCBB010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252392"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VII37_09065"
     CDS             complement(<1..792)
                     /locus_tag="VII37_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5069929.1"
                     /translation="MGIQLREFAAWARNRKILATLLVTVTLAIGMLIGTVISGHVGAA
                     HPAAGVGGTPLAIPDPVQLSSAFASIVSRVQPAVVNISTTQVIEQRGKGRTAPSQRNR
                     GGEMGPPDEQAPGQDQDQQPFQDFFNRFFDFPDQGPTAERSLGSGVIVDKKGFILTNN
                     HVVDQATKVQVTLNGEQTQYTAKIVGIDEETDLAILKIDVGHDLPFAKLGNSDSVQVG
                     DWALAIGNPFGFLQGSVTAGIISAKDRSNVGQQFQRFIQTDAAINP"
BASE COUNT          225 a          334 c          266 g          248 t
ORIGIN      
        1 gggattgatc gccgcgtcgg tctgaatgaa tctttgaaac tgctggccca cattgctgcg
       61 gtccttggcg ctgatgattc cagcggtgac gctgccttgc aggaacccga atggattacc
      121 aatggcgaga gcccagtccc ctacttgaac ggaatcggaa ttgccaagtt tcgcaaaggg
      181 aagatcgtgc cccacgtcga ttttcaagat cgccagatcg gtctcttcgt ctattcccac
      241 aatcttggcg gtgtactgag tctgctcccc gtttagcgtg acttgcacct tggtggcctg
      301 atccaccacg tgattgttcg tcaggataaa acccttctta tcgacgatca cgcccgagcc
      361 caggctgcgt tcggccgtcg gaccttgatc cggaaaatcg aagaagcggt tgaaaaagtc
      421 ctgaaacggc tgttgatcct ggtcctgccc aggagcttgt tcatccggcg gacccatctc
      481 gccgccgcga ttgcgctgcg atggcgccgt gcgccctttt ccacgttgtt cgatcacctg
      541 cgtggtcgaa atattcacga ccgccggctg cactctgctc acgatgctgg caaaggcgct
      601 cgacaactgc acgggatcgg gaatcgccag tggcgttcca cccactccgg cagcgggatg
      661 cgccgcaccg acatggccgg aaatcaccgt gccaattagc atgccaatgg ccagcgtcac
      721 ggtcacgagc aacgtcgcca gaattttgcg attgcgcgcc catgcggcaa attcccgaag
      781 ctgtatgccc ataatcttca actcctctta atccttgcgc tttctactat tcgccccgtt
      841 cgattcacgg agaattatac cactcactcc tccgctccgc gcatcctccg atgtgtcccc
      901 cgttttccag aaagcaaccg cacctcgaaa cgaatccgcc gcccggattg caaatttccg
      961 ctataactag agcttatggg actcacaatc attataaatt ggacctacac gccccaccgt
     1021 tgtcaaatgg accttccgcg agaacgtggg gaccgtgcaa ataatttgct tct
//
LOCUS       DATCBB010000821         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_90827, whole genome shotgun sequence.
ACCESSION   DATCBB010000821 DATCBB010000000
VERSION     DATCBB010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90827"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VII37_09070"
     CDS             complement(<1..510)
                     /locus_tag="VII37_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5069930.1"
                     /translation="MSSTVTHPAGGKTLDTAWVRQQFPSLQTQVNGQPAAFLDGPAGT
                     QVPHQVIDAIQNYLLHDNANTCGAFATSRRNDAMIAGARQAMADFFNCAPDEVSFGQN
                     MTTITFALARAIGRALNPGDEVIVTTLDHDANVAPWRALEEKGIVIRQVDIREQDCTL
                     DMEDLQRKLT"
     gene            700..1377
                     /locus_tag="VII37_09075"
     CDS             700..1377
                     /locus_tag="VII37_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5069931.1"
                     /translation="MPEIFSPTERTRVVREPHRGSFDREVIYKILDEGFVCHVGFTAE
                     GQPYVIPTMFARVGEAIYFHGSAASRMLRGVSGGLAVCVTVTLIDGLVLARSVFNHSM
                     NYRSVVALGKATLVSEADEKIVALRAFTGKILPGRWDDARQPSEKELKATSILRLPLS
                     EVSAKIRTGDVQDDEEDYALPVWAGVLPIRLAASEPIRDARCDAGLAVPTAVTNFDRR
                     LGGGNTA"
     gene            complement(1385..>2084)
                     /locus_tag="VII37_09080"
     CDS             complement(1385..>2084)
                     /locus_tag="VII37_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069932.1"
                     /translation="IDHLKSALEFGAHALTANKGPIVYAFEELTKLAVEKKKKFLYES
                     TVMDGVPIFSMFPLGLPAVDLRGFHGVLNSTTNVVLTEIEKGLSFDEAVKRAQAMGIA
                     ETDPSADIDGWDAAFKVVALAIVLMGIPIKIEQVRRTGIRELSAEKIRSVRDAGMRYK
                     LVCRAERRGLGVECTVAPELLLAGDPLANLEGSSSAIRFDMDVFGLSIVEHNPGIDAT
                     GYGLVADLVRAVQP"
BASE COUNT          397 a          503 c          684 g          500 t
ORIGIN      
        1 ggtgagcttg cgctgcagat cttccatgtc gagcgtgcaa tcctgctcgc gaatatctac
       61 ctggcggata actattccct tttcttctag ggcgcgccac ggtgcgacat tggcatcgtg
      121 atcgagcgtc gtgactatga cttcgtctcc agggttaagc gcgcggccga ttgcgcgtgc
      181 caatgcgaag gtgatggtgg tcatgttctg gccgaaactt acttcatctg gggcgcaatt
      241 gaaaaaatcg gccatggctt ggcgcgcgcc ggcgatcatg gcatcgttgc ggcggctggt
      301 ggcgaaggct ccgcaagtgt tggcgttatc gtgcaggagg taattttgta tggcgtcgat
      361 gacctggtga gggacttgcg tgcctgccgg gccatccaga aaagcggcgg gttggccgtt
      421 cacttgcgtt tgcagggaag gaaactgctg gcgcacccag gcggtgtcca aggtttttcc
      481 tccagcggga tgtgtaactg ttgagctcat ggtttctccg attgggtagt ttctcacaaa
      541 ggcggaaaga tgcgagcggt gagggatagt gggaagccac attggcggtt gcgggtggcg
      601 cgcggcggcg tgttagcatc gagttgatta ttgttcgtca ctcatgccgc tttggcgccg
      661 gctatcaact ctgcgccacg tttctcattg aggtgcatct tgccggaaat tttctcgcca
      721 actgagcgca cgcgggtggt gcgtgaaccg catcgcgggt cgtttgaccg cgaggttatt
      781 tacaaaattc tggatgaagg atttgtttgc cacgtgggat tcacggcgga ggggcagccg
      841 tatgttattc cgacgatgtt cgcgcgtgtg ggtgaggcga tttattttca cgggtcggca
      901 gccagccgca tgttgcgggg agtgagcggt gggcttgcgg tgtgcgtgac ggttacgttg
      961 attgatgggc tggtgctggc acggtcggtt tttaatcatt cgatgaatta ccgttcggtg
     1021 gtggcgctcg gtaaagctac gctggtgagt gaggcggacg agaaaatcgt ggcgttgcgg
     1081 gcttttactg gaaagatttt gccggggcga tgggatgatg ctcgacagcc cagcgaaaaa
     1141 gaattaaaag ctacgagcat cttgcggctg ccgctcagcg aagtttccgc gaagatacgc
     1201 accggcgatg tgcaggatga tgaagaggat tacgctttgc cggtgtgggc cggggtgctg
     1261 ccgattcgcc ttgctgcgag tgaaccgatt cgtgatgcgc ggtgcgatgc ggggcttgcg
     1321 gttccaacgg cggttactaa tttcgacagg cgattgggtg gcgggaacac agcataattt
     1381 acgtttatgg ctggacggcg cggactagat cggcgactaa tccgtagcct gtggcgtcga
     1441 tgccggggtt gtgttcgacg atcgacaggc caaaaacatc catatcgaaa cgaatcgcgg
     1501 agctggagcc ttctaaattc gcgagcgggt cgccagccaa gaggagctcc ggggctacgg
     1561 tgcattccac gcccaggccg cgccgttcgg ctcggcatac taatttgtag cgcatgccgg
     1621 catcgcgtac ggagcgaatt ttttcggcgc tcaattcgcg gatgccggtg cgccgcacct
     1681 gctcaatttt tattggtatg cccataagca cgattgccaa cgctaccact ttgaacgccg
     1741 catcccaacc atcgatgtca gcggaagggt cggtttccgc gatgcccatg gcttgggcgc
     1801 gcttgacggc ttcatcgaaa gagagacctt tctcaatttc ggtgagtaca acattggtag
     1861 tggaattcag gactccgtgg aagccgcgta agtccacggc cggcaggccc agcggaaaca
     1921 tggagaaaat gggcacgcca tccatgactg tggattcgta gagaaatttc ttttttttct
     1981 cgacggcgag cttggtcaac tcctcaaatg cgtagacgat ggggcctttg ttggcggtga
     2041 gcgcatgggc accgaactcc agcgcgcttt tcagatggtc gatg
//
LOCUS       DATCBB010000822         2802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_285276, whole genome shotgun sequence.
ACCESSION   DATCBB010000822 DATCBB010000000
VERSION     DATCBB010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285276"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..>2802)
                     /gene="treY"
                     /locus_tag="VII37_09085"
     CDS             complement(148..>2802)
                     /gene="treY"
                     /locus_tag="VII37_09085"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEY5069933.1"
                     /translation="FEVFVSELEKRGMGLLLDIVPNHMAASSENPWWMDVLENGPDSA
                     FSSYFDIDWHPPSRNLDNKILLPILGRPFGEALDSSELKLNFRDGQFSIEYFESSFPL
                     APRTYRSVLQHRIGRLKNLLDENSAAYQEYEGIVAALSAISDRDTTPVEAASDKRVRF
                     ESIRERLRQLAAGSQEVTQFIQENLADFNGKEGDPSSFANLQRLLGEQYYVLAFWQNV
                     NETINYRRFFTITDLVGMRVEDSLVFDATHTLILRLVSRGAVAGLRIDHIDGLRDPLA
                     YLKRLQDRITAEDGAKPGGEAYLLVEKILARHESLPDDWPVAGTTGYDYLNFANRILV
                     EPQGAKEIERIYSDFIGRGLKFDEVLYQKKKLVMGTILVVEMRSLGRQLGELSAQARY
                     ARDLPRHDLTEALIETTACFAVYRTYIRNLDLPSEATKVLEEALAQARLRKPNLNPQC
                     FDFLRDVLLLLNPPHVLPDHREARLSFVLRWQQFTGPIVAKGLEDTALYVYHPLVSLN
                     EVGGDPSPDTICPREFFEFIAERQKRWPHSMNASSTHDTKRAEDIRARISVLSEMPQE
                     WQKRLHHWAALNEKYKVAASGSTTVPDHNEEYFLYQTLLGMWPLEKSELGGVLERLQS
                     YAVKAIREAMVHTRWTRPNLAHEEGFTKFIAAILDEKQNNSFLQDFSNFHRDIAFYGM
                     LNSLSQTLLKITAPGVPDFYQGSELWDLRLVDPDNRGTVDFARRIEFLSSLKSNAPRE
                     TAVFASELLNNWTDGRIKLYLISKTLKCRQEFSELFTDGDFVPAKISGDRSQSVTAFF
                     RVWQNHQALVLAPKWLAGSGMEQDPTAQDKFWGNTSIGLPDTIATSWRNVLTGEPVAA
                     HGDQRGGLSVSQALKNFPVGLLLSNPTQ"
BASE COUNT          645 a          741 c          758 g          658 t
ORIGIN      
        1 cgctgatgca cacgacgatc cgcgcctttt gcttcaggca ggtgtccgcc gtcatgagac
       61 gaaatactca ttacttcgat gttacgttat ggttaaaaag caggcaatgg tcgccggaca
      121 caagctgtgc aaaaacagtc aggctattta ctgggttgga ttagataaaa gcaggcccac
      181 gggaaaattc tttaacgctt ggcttacact caggcccccg cgctgatccc catgggctgc
      241 gaccggctcg cctgtaagga catttcgcca actggtggct atagtgtccg gcaaccctat
      301 cgaggtgttt ccccagaact tgtcctgcgc ggtgggatcc tgctccatgc ccgaacctgc
      361 aagccatttc ggtgccagaa ccaaggcctg atgattttgc caaacgcgga agaacgcagt
      421 cacgctctgt gaacggtcgc cggatatttt tgccggcacg aaatcgccat ccgtaaagag
      481 ctcgctgaat tcctgccggc acttgagggt cttcgagatc aagtaaagtt tgattcggcc
      541 atcggtccag ttattcaaca attcgctcgc gaacacagcg gtctctcgcg gagcatttga
      601 tttgaggctg gataaaaatt caattcgacg cgcaaaatcc accgtcccgc ggttatctgg
      661 atctacgagg cgcaaatccc ataattccga gccttggtaa aaatctggaa caccaggcgc
      721 ggtgattttt agtaacgtct ggctaaggct gttcagcatt ccataaaatg cgatgtcgcg
      781 gtgaaaattg gaaaagtcct gcaggaacga gttgttttgt ttctcgtcga gaatggcggc
      841 gatgaacttt gtgaatcctt cttcgtgagc cagattcggg cgggtccagc gagtgtgcac
      901 catggcctcg cgaatcgctt ttacggcgta ggactgtaat ctttcgagca cgccgcccag
      961 ttcgcttttc tccagcggcc acatgcccag cagcgtctgg tacagaaaat attcttcgtt
     1021 gtgatcggga accgtcgtgc tgccgctggc agcgaccttg tatttctcat ttaaggcggc
     1081 ccagtgatgt aagcgtttct gccactcttg cggcatctca gacaaaacgc ttatgcgcgc
     1141 gcgtatatcc tcagcccgct tggtatcgtg cgtggaactg gcattcatgg agtgcggcca
     1201 tcttttttgg cgctcggcga tgaactcaaa aaattcccgg gggcaaatgg tatcgggcga
     1261 tgggtcgccg ccaacttcgt taagtgacac tagcgggtgg taaacgtaca gcgcggtgtc
     1321 ttcgaggccc ttcgccacga tcggccccgt gaattgttgc cagcgcaaaa cgaagcttag
     1381 acgggcttcg cggtgatccg gcaaaacgtg cggcggattc agcagcaaca acacgtcgcg
     1441 cagaaaatca aaacattggg gattcaaatt cggcttgcgt aaacgcgctt gagccagcgc
     1501 ctcctccaga accttggtgg cctcggaggg tagatccaaa ttacggatat acgtgcggta
     1561 caccgcaaag caggcggtcg tttcaatgag cgcttcggtc aaatcgtggc gtggcaaatc
     1621 acgagcgtag cgggcttggg ccgacaattc gccaagttgg cggccaaggg acctcatttc
     1681 cacaaccaga atcgtgccca ttaccagttt tttcttctgg tacagcacct catcgaattt
     1741 caggccgcgg ccgataaaat cggaataaat ccgttcaatc tcttttgcgc cctgtggctc
     1801 aaccaaaata cgatttgcaa agtttagata gtcataaccg gttgtgccgg ccaccggcca
     1861 atcgtccggc aggctttcat gccgcgcgag aatcttttca acgagcaggt acgcttctcc
     1921 gccgggcttc gctccatctt ccgccgtgat gcgatcctgc agtcgcttga ggtaggccaa
     1981 cggatcgcgc aatccgtcaa tgtgatctat gcgcagtccc gcaaccgcgc cgcgagagac
     2041 gagccgcaaa attaaagtgt gggtggcgtc gaatactaaa gaatcctcga cgcgcatgcc
     2101 caccagatcg gtaatggtaa aaaaacggcg gtaattaatg gtttcgttga cgttctgcca
     2161 gaaggcgaga acgtaatatt gttccccgag cagccgttga agattggcga aactgctggg
     2221 gtcgccttcc ttgccgttaa aatcggcgag gttttcctgt ataaattgcg taacttcctg
     2281 gctgcctgcc gccaattgcc gtaaacgctc gcgtatggac tcgaaacgca cgcgtttgtc
     2341 ggaggcggcc tcgacaggtg tagtatcgcg gtctgagatt gccgaaagcg cggcgacgat
     2401 accttcgtat tcctggtagg cagcggagtt ttcatccagg agatttttta acctgccaat
     2461 gcgatgttgc aaaacggagc gataggttcg cggcgccaag ggaaacgacg attcgaaata
     2521 ttcgatggag aattggccgt cgcgaaaatt aagtttcagt tcgctgctgt cgagcgcctc
     2581 cccaaatggc cgcccaagta ttggtaaaag tattttgttg tccagatttc gcgaaggcgg
     2641 gtgccaatct atatcgaagt aagaagaaaa ggccgagtcc gggccgttct ccaaaacgtc
     2701 catccaccag ggattttcgc tgctggcggc catgtggttg ggcacgatat ccaggagcaa
     2761 tcccatgccg cgtttttcca gctcgctgac gaaaacttca aa
//
LOCUS       DATCBB010000823         2726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_26562, whole genome shotgun sequence.
ACCESSION   DATCBB010000823 DATCBB010000000
VERSION     DATCBB010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26562"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VII37_09090"
     CDS             <1..558
                     /locus_tag="VII37_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5069934.1"
                     /translation="TDATYEQARAAIAHGAHHAVHVYNAMRPFSHRDSGVIGAILTTP
                     NVTAELIADGVHVEEAAMRLLLQAKSSDCVILVSDGISATGMPDGKYVLGQIEVTVTG
                     GVCRNSEGKLAGSTLTLDKALRNVMALGKSFADTTRMLTLNPAGLLGVEFKKGALRVG
                     ADADVVLMDHAMQISGVWVRGMAVN"
     gene            complement(589..846)
                     /locus_tag="VII37_09095"
     CDS             complement(589..846)
                     /locus_tag="VII37_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY5069935.1"
                     /translation="METITMYTTAWCPDCRRAKSFMKERGVEFREVDIEQDPTGEEIV
                     MQANNGKRKVPTLQIGDHYFACSPFNPVALASELKIPLNKS"
     gene            complement(925..1365)
                     /locus_tag="VII37_09100"
     CDS             complement(925..1365)
                     /locus_tag="VII37_09100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY5069936.1"
                     /translation="MTLPEAIELIIVTPQRQLLREKVASVQLPGASGELGILPGHAPL
                     ITELGNGELSYKASGGGEPVVLAVLSGFAEVLPDRVTVLAETAERPEEIDVDRAKQAL
                     ERAEKRLASTDADVDWDRATVALQRALARIKVGTVHRRVHTHSS"
     gene            complement(1384..>2726)
                     /gene="atpD"
                     /locus_tag="VII37_09105"
     CDS             complement(1384..>2726)
                     /gene="atpD"
                     /locus_tag="VII37_09105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY5069937.1"
                     /translation="TSEGFNIPEPLDVTAEVQQHLGEGRVRAVAMEAVEGMVRGMPAE
                     DLGQGITVPVGRATLGRVMNVLGKPVDQLGPINSPTHYPIHRAAPPLEDQSTGLEMFE
                     TGIKVVDLMEPYVKGGKIGLFGGAGVGKTVLILELIHNVAMQHGGLSVFAGVGERTRE
                     GNDLWLEMSESGVIVPGNTEKSRAALIYGQMTEPPGSRLRVGLTGLTVAEYFRDEEHL
                     DVLLFIDNIFRFVQAGAEVSALLGRMPSAVGYQPNLNTEIGELQERITSTKNGSITSV
                     QAIYVPADDYTDPAPAATFAHLDATTNLSRQIVERGIYPAVDPLASFSRILDPRIVGA
                     EHYSVARGVKSILQRYKDLQDIIAILGIEELSDDDKRTVARARKIEKFLSQPMFVAAQ
                     FTGLEGKYVKIEDTVRSFKEIVEGKYDDVPEQAFYMVGTIDEALKKAETMKSNA"
BASE COUNT          588 a          790 c          740 g          608 t
ORIGIN      
        1 accgacgcaa cttacgaaca ggctcgcgcc gccatcgccc acggcgcgca tcacgcagta
       61 catgtttaca acgccatgcg gcccttttca caccgcgaca gcggcgtaat cggcgccatt
      121 cttactaccc caaacgttac cgcggaatta attgcggatg gcgtgcacgt tgaagaagca
      181 gccatgcgtt tactcttaca agccaagagc tcggactgcg taatcctggt aagcgatgga
      241 atttcagcca ccggaatgcc ggacggaaaa tacgtgctgg gacaaattga agtcaccgtt
      301 acgggagggg tgtgccgaaa ctccgaagga aaattggccg gcagcacgct cacgctggat
      361 aaggctttga gaaatgtgat ggcgctgggc aagtcatttg cagacaccac acgtatgctc
      421 acgctaaacc cggctggctt acttggcgtg gaattcaaaa agggcgcgct gcgcgtaggt
      481 gcggacgcgg acgtggtgct gatggaccat gccatgcaaa tttcgggcgt ctgggttcgt
      541 ggaatggccg taaattgaca gattcaaatc taacgatctg acagacgact aggacttatt
      601 gagcgggatt tttaattctg aggccagcgc caccggatta aacgggctac acgcgaaata
      661 gtgatcacct atctgcagcg tgggaacttt tcgtttgccg ttgttggctt gcatcacgat
      721 ttcttcgccc gtgggatctt gctcgatatc cacttcgcgg aattccaccc cacgttcctt
      781 cataaacgat ttagcccgac gacaatccgg acaccaggcg gtggtgtaca tcgtgatggt
      841 ttccatgaaa attcgtcctt tgagtggtgg gaagacccgg ggtagcccaa tcgacttcta
      901 tatcagttta acctaaacga ggcactagct gctgtgtgtg tgcacacggc gatggacggt
      961 gcccacctta atgcgcgcca atgcgcgctg caacgcgacc gtggcgcgat cccaatcgac
     1021 atccgcatcg gtggatgcca gccgcttctc tgctcgttca agggcttgtt tggcgcgatc
     1081 aacgtcaatt tcttcggggc gctcggcggt ttccgccagc accgtgacgc ggtcgggtaa
     1141 cacttccgca aatccactga gcaccgcaag aacaacaggc tcgccgcctc cggacgcctt
     1201 gtaactcagc tcaccgttgc cgagttccgt gatcagcggc gcgtgacccg gcagaatgcc
     1261 gagttctcca ctagcgccgg gaagttgcac gctggcaact ttttcgcgca gcaattgcct
     1321 ctgtggcgtg acgatgatca actcgatggc ttcgggaagc gtcatgatct tctatatttc
     1381 ggtttacgcg ttgctcttca tggtttcagc cttcttcaag gcctcatcga tcgtgcccac
     1441 catgtaaaaa gcctgctctg gaacatcatc gtatttgcct tccacgattt ccttgaagct
     1501 ccgcacggtg tcttcaatct tcacgtactt tccttcgagc ccagtaaact gcgcggccac
     1561 gaacataggc tgcgaaagga atttttcgat tttgcgcgcg cgtgccacag tgcgcttatc
     1621 atcgtcggaa agttcttcaa taccgagaat ggcgatgatg tcctgcaaat ccttgtagcg
     1681 ctgcaaaatg gatttcacgc cgcgagccac cgagtaatgc tcagctccca cgatgcgcgg
     1741 atcaagaatg cgcgagaaac tggctagcgg atcaaccgcc gggtaaatgc cgcgttccac
     1801 aatctggcgg ctaaggttcg tcgtggcatc caggtgcgcg aacgttgccg ctggagctgg
     1861 atcggtatag tcatcggctg ggacataaat cgcttgcacc gaagtgatag atccattctt
     1921 ggtggaggta atgcgctcct gcaattcacc gatttcggtg ttgaggtttg gctgatatcc
     1981 cacagccgat ggcatgcgtc ccagaagtgc ggaaacttcc gcgcctgctt gcacgaagcg
     2041 aaaaatattg tcgatgaaca gcagcacgtc gagatgttct tcgtcgcgga aatattccgc
     2101 caccgtcagt cctgtcaacc cgacgcgcag acgcgatccg ggcggttcgg tcatctgccc
     2161 gtagattaat gcggcacgcg atttttccgt gttgcctgga acgatgacgc ccgactcgga
     2221 catttccagc caaaggtcgt tgccctcgcg cgtccgttcg cccactccag cgaacaccga
     2281 aagaccgcca tgctgcatgg ccacgttatg gatgagctcc agaatcaaaa cggttttgcc
     2341 gacgccggca ccgccgaaca atccgatctt tccgcccttt acataaggct ccatcagatc
     2401 gacgacttta atgccggttt caaacatctc gagcccggtg gattgatctt ccagaggggg
     2461 cgcggcgcga tgaatcggat agtgcgtggg gctattgatg ggcccaagct ggtccaccgg
     2521 cttgcccaac acattcatca cccgcccaag cgtggcgcgg ccaaccggaa cagtgatgcc
     2581 ttgtcccaaa tcttccgcgg gcatgccgcg aaccatgccc tcaacggcct ccatggctac
     2641 cgcgcgcacg cggccctcgc caaggtgctg ctgcacttcc gcggtgacgt ccagcggctc
     2701 aggaatattg aacccttcgc tggtga
//
LOCUS       DATCBB010000824         1025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_123655, whole genome shotgun sequence.
ACCESSION   DATCBB010000824 DATCBB010000000
VERSION     DATCBB010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123655"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1022
                     /locus_tag="VII37_09110"
     CDS             222..1022
                     /locus_tag="VII37_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5069938.1"
                     /translation="MAIENCPLCRGTGWRLEQRLDGAPGKVATACDCGMEERAARVME
                     RARIPKRYEHCDFESYVADLTDGKTWMPQHAQSMKQAKMLTQGFVRDYPAAAEKGLLL
                     MVPSGVGKTHLAVAALKELIRRGHAGLFCDYRELLKEIQASYNPASESTEMRILEPIR
                     NVEILVLDDLGASKPSDWVREIVGIVLNARYNENRTTVITTNYVDNPVSEGEPSRLPG
                     GRMIPASREDTLEQRIGSRMRSRLYEMCRTVEVIAPDFRREKTQAGHT"
BASE COUNT          234 a          245 c          342 g          204 t
ORIGIN      
        1 ccagcaggaa gaggagacca ccccaggacc acgcgaggat ctcgctgcgg acacgagcgc
       61 ttacagatga agaggcctgg gaaggtacgg gcttggtttt agctttcagt ttgcaccatc
      121 gtgaatttgg gaatattagc acgccgctgt cgcgagctgt ttaacggcga cggaagtaca
      181 taacttgctg tggcgggtgc gtgctcgtat gctgagtcaa tatggcgata gaaaattgtc
      241 cgctttgtag agggaccggg tggcggctgg agcagcggct ggatggcgcg ccgggtaaag
      301 tcgcgacggc gtgcgattgt ggaatggaag aacgcgctgc gcgggtgatg gaacgtgcgc
      361 gtatacccaa gcgctatgaa cactgcgatt ttgaaagcta tgtcgcggat ctgacggacg
      421 gcaagacctg gatgccgcag cacgcgcagt ccatgaagca ggccaagatg ttgacgcagg
      481 gatttgtgcg ggattatccg gcggcagcgg aaaaagggtt gctgctgatg gtgccgtcgg
      541 gcgtgggcaa aacgcatttg gccgtggcag ctttgaagga attgattcgc cgcgggcacg
      601 ctgggctttt ttgcgattat cgcgaactgt tgaaagagat acaggccagc tacaacccag
      661 ccagcgaatc gacggagatg cgcattctgg aaccgattcg caacgtggaa attttggtgc
      721 tcgacgatct gggagcgagc aagccttcgg attgggtgcg cgaaattgtg ggcattgtat
      781 tgaacgcgcg ctacaacgag aaccgcacta cggtgatcac cacgaactat gtggataacc
      841 cggtgtcgga aggcgaacct tcccgccttc caggcggccg gatgatacct gcgtcgcgcg
      901 aagatacctt ggagcagcgc atcggaagcc gaatgcgttc ccggttatat gaaatgtgcc
      961 gcaccgttga agtaattgcg cctgatttcc gccgggaaaa gacacaggcg ggccacacgt
     1021 aaggg
//
LOCUS       DATCBB010000825         3091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_221890, whole genome shotgun sequence.
ACCESSION   DATCBB010000825 DATCBB010000000
VERSION     DATCBB010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221890"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VII37_09115"
     CDS             <1..605
                     /locus_tag="VII37_09115"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY5069939.1"
                     /translation="GEPSAHGTYVRYNADEMLAILALESARAKTFVVGEDLGTVDEGV
                     REKLAEYAVLSYRLLWFEEKQPEHYPKLAVAAVSTHDLPTIAGIWSGADFEEQSQLGL
                     EPSRDNSDKMRMYLKRATHVDDHEPIADVIVKTHEALARSPAAITLASVEDGVGADKR
                     PNLPGAPEDRRPNWCIPLPKKLEEIESDPTILRIAASLKR"
     gene            complement(638..1960)
                     /locus_tag="VII37_09120"
     CDS             complement(638..1960)
                     /locus_tag="VII37_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY5069940.1"
                     /translation="MRSRFRELQQLRPNVLSPYIVLAILAFAVALPPATATDNKAPQE
                     WIATWMTANLDLSNQEMAVVSSASHDLTEFNNQTIRGIIHTAIAGRAIQIRLDNTFGT
                     RPITFDAVFVGKQKQGASLLPGSNHVVIFGGAKTVTIPEGSEALSDEIPMAVDAQQNL
                     AISVYTSKPTGPATVHRSAFQTNYVSDAGNFAAQESAQPFSKTTASWFFLKEVDVLAA
                     KEAAGAIVALGDSITDGASSRTDAYDRWTDVLARRLNANSPHQAFSVLNAGIGGNRVL
                     SSSPCFGENALARLNRDVFAQAGVRAVILFEGTNDIGQPDTPVDAKFAACLARTHISA
                     DDLIAGYKQIITQVHARHLKIFGATILPFQGFKGWTEKGEAVRKATNQWIMESHAFDG
                     VIDFASALADPANPARLATQYDSGDHLHPSSAGHEAMARAIDPKLFTK"
     gene            2103..>3091
                     /locus_tag="VII37_09125"
     CDS             2103..>3091
                     /locus_tag="VII37_09125"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEY5069941.1"
                     /translation="MCGFSRRLTWLLLAACSSLFFMDGAYGADDVLARTPPMGWNSWN
                     KFGCNVSEQLIRDAADAMVSSGMQAAGYEYVVIDDCWQVGRDSNGNILPDPQRFPSGI
                     KALADYVHGKHLKFGLYSDAGTKTCAGRPGSRGYEFQDARQYAAWGVDYLKFDWCSTD
                     TENAEAAYTTMHNALKASSRPIVFSICEWGKSKPWLWAKDVGNVWRTTGDIFDCWDCK
                     KEWSTGWVVILDQQVALESFAGPGHWNDPDMLEVGNGGMSTTEYRSHFSLWSMLAAPL
                     IAGNDLKNMTPEIKEILTNKEVLAVDQDALGMQGRRTSKNGDLEVWVKQLHDG"
BASE COUNT          730 a          702 c          957 g          702 t
ORIGIN      
        1 tgggtgagcc gagcgcgcat gggacgtacg ttcgttacaa tgcggacgag atgctcgcga
       61 ttctcgcgct ggagagtgct cgagcaaaaa cgtttgtggt tggagaagac ctcggaacag
      121 tggacgaagg tgtgcgagaa aaacttgcgg agtatgcggt gctttcttac cggcttttgt
      181 ggtttgaaga aaagcagccg gagcattatc cgaagttagc tgtcgcggcg gtgtccacgc
      241 acgatttgcc gaccatcgcg gggatctggt ccggtgcgga ttttgaggaa caaagtcaac
      301 tgggactgga acccagcaga gacaattccg acaaaatgcg gatgtacctc aagcgggcga
      361 cccacgtgga tgatcacgaa cctattgcag atgtaattgt taaaacgcac gaagcacttg
      421 cgcgatcacc ggcggccatt acgctggcat ccgtggaaga tggcgtgggc gcggacaaaa
      481 ggcccaatct gcctggagcg cccgaagata gacgtcccaa ctggtgtatt cctttgccca
      541 aaaaacttga agaaatcgag agcgatccga cgattctaag aatcgcggcg agtctgaaga
      601 gataagcaag accgtgcggc gatttgcgaa ttcggcgtta cttagtaaac aattttggat
      661 ctatggcccg cgccatggct tcatggcctg cggagctagg gtgcaaatga tcgccgctat
      721 cgtattgcgt ggctaggcgc gcggggttgg cggggtcggc taatgctgat gcgaagtcga
      781 tgactccatc gaaggcgtgc gattccataa tccattgatt tgttgctttg cgaacggctt
      841 cgcctttctc cgtccaacct ttgaagccct gaaatggaag aatcgtggcg ccgaagattt
      901 tcaagtgacg cgcatgaacc tgggtgatga tttgtttgta gccggcaatc aaatcgtcgg
      961 ctgagatatg agtccttgct agacatgcgg cgaatttcgc atcaaccggc gtgtcgggct
     1021 ggccgatatc gttggtgcct tcgaacaaaa ttaccgcacg cacacccgcc tgagcaaaaa
     1081 catcacgatt gagtctggcg agcgcatttt ctccaaaaca tggtgagctg ctcaacacgc
     1141 gattgccgcc gatgccggcg tttaaaacgg agaaggcttg atgcggagaa tttgcgttga
     1201 ggcggcgagc aagaacgtct gtccagcggt cgtaggcgtc ggtgcgtgac gatgcgccat
     1261 cggtaatgga gtcgcccaaa gcgacgatgg cgccagcggc ttctttggct gcgagcacat
     1321 ccacctcttt caaaaagaac caggaagcgg tggttttgct gaaaggctgg gcactttctt
     1381 gagcggcaaa gttgccagcg tccgaaacgt agttggtttg aaaagcggag cggtggactg
     1441 tggcggggcc ggtgggcttc gaggtgtata cgctgatggc cagattctgc tgagcgtcga
     1501 ctgccatcgg aatttcgtcg ctaagcgctt cgctgccttc tggaatagtg acagttttcg
     1561 ctccgccaaa tattacgaca tggttcgatc cgggtaatag cgatgcacct tgtttttgtt
     1621 ttccgacaaa caccgcatcg aaagtgatgg gacgtgtgcc aaacgtgttg tccagacgaa
     1681 tctgaatagc gcggccggca atggctgtgt ggatgatgcc tcgaatggtc tgattgttga
     1741 attccgtaag gtcgtgagaa gcacttgaaa ccacggccat ttcctgattt gacaggtcta
     1801 agttcgcggt catccaggtc gcgatccatt cttgtggcgc tttattgtcg gtcgcggtgg
     1861 caggcgggag cgctacagca aatgcgagaa tggccaagac aatataggga ctcaacacat
     1921 taggacggag ttgttgcaat tctcgaaagc gagatcgcat ttttttcctt tgccgatgtg
     1981 ctgcgcgatt tttgctcggc tgttgtgact cataaccgag cagcagcgaa acgcatatac
     2041 aaattgaata gaagcactag aatattacct ccagtagcgt ttcaaatcag gggaatccaa
     2101 taatgtgcgg cttctcaaga cggctgacat ggctgttgtt ggcggcatgc agttcacttt
     2161 tttttatgga tggcgcgtac ggcgccgatg acgtgttggc gcgcactccg ccgatgggat
     2221 ggaatagttg gaataagttt ggctgcaatg taagtgagca gttgattcgc gacgccgcgg
     2281 acgccatggt aagcagtgga atgcaggctg ccggatatga atacgtagtg atcgacgact
     2341 gctggcaggt ggggcgcgac agcaacggga atatcctgcc cgatccgcaa cggtttccat
     2401 ctggcatcaa agccctggca gattatgttc atggcaaaca tttgaagttc gggctttatt
     2461 cggatgccgg aactaaaaca tgcgcgggac ggccgggaag ccgggggtat gaatttcagg
     2521 acgcgcggca atatgcggca tggggcgtcg attatttgaa attcgattgg tgcagcacgg
     2581 acaccgaaaa cgccgaagcc gcttatacga cgatgcacaa cgcgctgaag gcttcaagcc
     2641 ggccgatcgt tttcagcatt tgcgagtggg gaaaaagtaa accgtggctg tgggcgaaag
     2701 atgtcggcaa tgtgtggcgc accacgggag atatttttga ttgttgggat tgcaagaaag
     2761 agtggtccac cgggtgggtg gtgattctgg atcagcaagt cgcgctggag tcttttgctg
     2821 ggccgggcca ctggaatgat ccggacatgc tggaagtcgg caacggaggg atgagcacga
     2881 ctgaataccg gtcgcatttc agtttgtgga gcatgttggc tgcgccgctg attgcgggga
     2941 acgatctaaa gaatatgacg ccggagataa aagaaatcct gacaaataaa gaggtgctgg
     3001 cggtggatca ggatgcattg ggcatgcaag ggcggcgcac gagcaagaat ggcgaccttg
     3061 aagtgtgggt aaagcaactt cacgacggga a
//
LOCUS       DATCBB010000826         1683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_58909, whole genome shotgun sequence.
ACCESSION   DATCBB010000826 DATCBB010000000
VERSION     DATCBB010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58909"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VII37_09130"
     CDS             complement(<1..549)
                     /locus_tag="VII37_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5069942.1"
                     /translation="MAVFSRAKELEREGRSIIHLELGEPDFHPAAPVVDALRDAAASG
                     RDRYVSTRGIPALRAAIASYLRRTRRLEVSSEEVLVAPGCKMALSLAMMALIEPGAEV
                     LYPDPSFPIYPSFTRGLGATAVPFGLEEKNNFQPDLEEIAAKITPRTSVLIFNSPNNP
                     TGTVLSRETLEQLAELARKHDLW"
     gene            complement(625..1173)
                     /locus_tag="VII37_09135"
     CDS             complement(625..1173)
                     /locus_tag="VII37_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5069943.1"
                     /translation="MSSEASRIKFLERDAVSPEAAALYDALIAQRGVVPNMFKVLAYS
                     PDIAKGVAGFLKPLLSDGALSGWYKELVAVRISILCESEYAIRAHSISAQKKGASPEQ
                     IAAVADFEKGPYSEKEKLGFRLADRLHRGGRQVDDTFYAQLQAVFQDAALVELFLTAA
                     AFEMFPRFIDGLRIPVTPPAAK"
     gene            complement(1291..>1683)
                     /locus_tag="VII37_09140"
     CDS             complement(1291..>1683)
                     /locus_tag="VII37_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069944.1"
                     /translation="GGTIAAGNTSGSIISRVNNGYLNRANFTFAPPADPAGCALDPNA
                     CTTAFGNLARNIYRGPFQQNWDFSLIKNFHITERQQLRFTTDFFNIWNHANFANPTFT
                     DISNPAFGKIFSTVGTPRLIQFSLRYAF"
BASE COUNT          421 a          450 c          458 g          354 t
ORIGIN      
        1 ccacaagtca tgttttcgcg ccagctcagc gagttgctca agcgtctcgc ggctgagcac
       61 cgtacctgta ggattgttgg gagaattaaa aatcaacacg ctggtgcgcg gtgtgatctt
      121 cgcggcgatt tcctcaagat cgggctgaaa attatttttt tcttccagtc caaacggaac
      181 cgccgtggcc ccaagtccac gggtaaacga aggataaatg ggaaagctgg gatccggata
      241 tagaacttca gccccgggct caatgagcgc catcatggcc agcgataaag ccattttgca
      301 acctggagcg accagcactt cttcgctact tacttccaat cgccgggtgc gccttagata
      361 actagcaatg gcagctcgca aagcagggat gccgcgcgtg gatacatagc ggtcccgccc
      421 tgaagccgcg gcgtcacgca gcgcatcaac taccggagcg gctggatgaa aatctggctc
      481 gccaagttcg aggtgaatga tggagcggcc ttcgcgctcg agttccttgg cgcgcgaaaa
      541 aacggccaat gcaccttcac cggttaattg agaaacgcgt gttgcgacgg ggcgcatagg
      601 ttaaaagttc cgcgaggaat attatcactt cgcagcgggc ggagtgacag gaatgcgcaa
      661 gccgtcgata aaccgaggaa acatttcaaa agctgccgcc gttaagaata actcgacgag
      721 cgcggcatcc tggaagacag cctgaagctg cgcgtaaaag gtatcgtcta cctgtcggcc
      781 gccgcggtgc aaccggtccg caaggcgaaa acccagcttt tctttttcac tgtacggtcc
      841 cttttcaaaa tcagcaacgg cagctatctg ctctggggaa gcacctttct tctgcgctga
      901 aatactgtga gcgcggatgg catactccga ttcgcaaaga atagaaatgc gcaccgccac
      961 gagttcctta taccatcctg acaatgcgcc atcgctcagc aaaggcttca aaaaaccggc
     1021 cacacctttg gcaatgtccg gcgagtacgc cagcactttg aacatgttgg gaacaacgcc
     1081 acgttgagcg ataagcgcat cgtatagagc cgcggcttcc ggcgagacgg cgtcacgttc
     1141 caggaatttg atgcgagaag cttcgctgct catgttgacc tcaatttgtt cgccaagggc
     1201 acgtcgctac tgtcaatcac agaacaccca cacgcaagtg cagcgtaccg cagccactct
     1261 gtacttcgct agattagccg gccagcccaa ttaaaaagcg tagcgcagag agaattgaat
     1321 caaacggggc gtccctacag ttgagaaaat ttttccgaag gcagggttag agatgtccgt
     1381 aaacgtcggg ttggcaaaat tcgcatggtt ccagatattg aagaaatccg tcgtaaagcg
     1441 cagttgttgt cgctcggtaa tgtggaaatt cttgatcaac gaaaaatccc agttctgctg
     1501 gaaaggtccg cgataaatat tgcgcgcgag attcccgaac gccgtggtgc aggcattggg
     1561 atccagcgcg catccagcag ggtcggctgg cggcgcgaaa gtaaagtttg cgcgattcag
     1621 atatccatta ttcacgcgcg agatgatact tccggaagtg ttgcccgcgg cgatcgttcc
     1681 gcc
//
LOCUS       DATCBB010000827         1881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_482195, whole genome shotgun sequence.
ACCESSION   DATCBB010000827 DATCBB010000000
VERSION     DATCBB010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482195"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VII37_09145"
     CDS             complement(<1..270)
                     /locus_tag="VII37_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069945.1"
                     /translation="MKILIRATNWVGDAIMALPAIRAIRNHPDAHVSILARSYVADIY
                     RDQNVAHELIIYDHRGKHAGISGRERLASELRSRKFDVAILLQNAF"
     gene            complement(267..1268)
                     /gene="lpxK"
                     /locus_tag="VII37_09150"
     CDS             complement(267..1268)
                     /gene="lpxK"
                     /locus_tag="VII37_09150"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY5069946.1"
                     /translation="MMLSRPLRILLWPASKLYGVGVSIRTRLYLQGLLKQNRLNAAVI
                     SIGNLSVGGTGKTPLVMWLAEKLLAQGHRVAILTRGYRGTAGSSDEVELMRSRLQNRV
                     IFGVGKNRFAEGQRIESHQAIDIFLLDDGFQHLQLARDVDIVLMDASRPIAQPLLPAG
                     PMREPPSALARAGIIIFTRTENSAGAKEAIQRLSNLPIFAASTHLLGFRRTGIEAPLH
                     SVADLGSGPFFICCGIGNPEAFVRDLQRWGIMPAGHMFFPDHHRYTREDVQAIEGAAA
                     RLGAKSLLTTEKDAWNLKDVQFSTLPVFVSVIDLEITAETEFLAAINGALNARGART"
     gene            complement(1265..>1881)
                     /locus_tag="VII37_09155"
     CDS             complement(1265..>1881)
                     /locus_tag="VII37_09155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5069947.1"
                     /translation="GSVVATEEPLALIAFGTLQGEFPNALLVLAPRKPERFDDAADFI
                     HESRRKFLRRSNLALQSSNNGDHSAVPEIPKDVTVLLLDSIGELASLYRCADGVFVGG
                     SLVPSGGHNILEPAAFGKIPVFGPSMENFSEIASRFVSAGAAVQVESPEDAGVAWIEF
                     FRDPERMRKMGESARSLVEASRGATDRALAVISGFLNEHGAPRR"
BASE COUNT          437 a          487 c          568 g          389 t
ORIGIN      
        1 gaaagcgttt tggagaagaa tggcgacgtc gaatttccga gagcgcagtt cggaggcgag
       61 gcgttcgcga ccggaaattc cggcgtgctt gccgcgatga tcgtaaatga tcaactcatg
      121 ggcgacgttt tgatcgcggt agatgtcggc cacataggag cgggccaaaa ttgagacgtg
      181 ggcgtcaggg tgattgcgaa tggcgcggat tgcaggcaag gccatgatgg cgtcgcccac
      241 ccaattggtg gcgcgtatga gaattttcat gtgcgggcac cgcgtgcatt aagtgcgccg
      301 ttaatggcgg cgagaaattc agtttcggcg gttatttcca gatcgatgac ggaaacaaaa
      361 acaggcaagg tagaaaattg cacgtccttc agattccaag catctttttc ggtagtcagc
      421 agcgactttg cgccgagtcg ggccgcggct ccttcgatcg cttggacgtc ttcgcgagtg
      481 tagcggtgat ggtcaggaaa aaacatatgg ccagcgggca ttatgcccca gcgttggagg
      541 tcgcgcacaa atgcttcggg atttcctatg ccgcaacaaa tgaagaaggg gccactacca
      601 aggtctgcaa cggagtgcag cggagcctcg atgccggtgc ggcgaaagcc aagtaaatga
      661 gttgacgctg caaaaatcgg caagttggat aagcgttgga tggcttcttt ggcgccggct
      721 gagttttctg tgcgggtaaa gatgatgatg ccagcgcggg ctagtgcgga tggaggctcg
      781 cgcattggtc ctgcgggaag taagggctgc gctatgggac gcgaggcgtc catgagcact
      841 atatccacgt cgcgagctaa ttgaagatgt tggaaaccat cgtctagcaa gaaaatgtct
      901 atggcttggt gtgattcgat gcgttggcct tcggcaaagc gatttttgcc aactccaaag
      961 atgacgcgat tttgcaaacg cgagcgcatg agctcgactt cgtcgctact gccggcggtc
     1021 ccgcgatagc ctcgggtgag aatggcgacg cggtggcctt gggccaggag cttttctgcc
     1081 agccacatta ctagtggagt tttgccggtt cccccaacgg ataaattacc gatgctgatg
     1141 actgcggcat tgagacgatt ctgttttagc aaaccttgaa gatagagccg cgttcgaatc
     1201 gaaacgccga caccgtagag tttagaagcg ggccacaaca aaatgcgaag gggccgcgat
     1261 aacatcatcg ccgcggcgca ccgtgctcgt tcaagaatcc cgagatcact gcgagcgcac
     1321 ggtccgtggc gccacggctg gcttcaacca gcgagcgggc agattcgccc atctttcgca
     1381 tgcgctcggg atcacggaaa aattcaatcc acgccacgcc ggcgtcttcc gggctctcta
     1441 cctgtacagc ggcaccagcg gaaacaaacc gcgatgcaat ctccgagaag ttctccatgg
     1501 acggcccaaa cacgggaatt ttgccgaacg ccgcaggctc cagaatatta tgaccgccgg
     1561 agggtaccag cgaaccgcct acgaacacac cgtcagcgca gcgatagaga gaggccaatt
     1621 ctcctatgct gtcgagcaac agcactgtta catccttggg gatctctggc acggcagaat
     1681 ggtcgccatt gtttgaactc tgaagagcta aatttgagcg tcgcagaaat ttacgacgcg
     1741 attcgtgtat aaaatcggcg gcatcatcga atcgctctgg ctttcgcggc gccaacacca
     1801 gcagcgcgtt tggaaactcc ccctgcagcg taccgaaagc gatgagtgcc aggggttctt
     1861 cggtggccac cacgctgccc g
//
LOCUS       DATCBB010000828         1559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_221969, whole genome shotgun sequence.
ACCESSION   DATCBB010000828 DATCBB010000000
VERSION     DATCBB010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221969"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..615)
                     /locus_tag="VII37_09160"
     CDS             complement(10..615)
                     /locus_tag="VII37_09160"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY5069948.1"
                     /translation="MKQGADNFVAAGFGKEFAWLDLANSLEWNGYGKVTDHLKDSAWA
                     AHIVKHWNLASTLPRPIPLAQLIALRALLRRMAEKLIAGESIDERDLSALNSYLNVPV
                     RVKLFQHQNGVQSELVPLRPGWPWILSRIAASFAEMLAMNRPDRLKYCPNEGCKWIFY
                     DRTKANTRRWCNDRTCGNRDRVQRSRVRQRKIAEKSKSSSD"
     gene            759..>1559
                     /locus_tag="VII37_09165"
     CDS             759..>1559
                     /locus_tag="VII37_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5069949.1"
                     /translation="MSATITKSATLNSDTSQKAAASNRLLIIFCFFAIYAIWGSTYLA
                     IRYAVETIPPLYAAGIRHLTSGTILLLWCFAKKVRPTAAQIRTGVVIGILFFLLGHGP
                     LHWAETRVPSGLASLLVATEPIFVFFLAALASKRWQFSWKIFAGVFLGLAGVGLLVGR
                     TTLTSSKGMIVASLAILFGALSWSAGIIYARKSDLAGHPLLMTALSSLSGGILLLSSA
                     TIFGEWRGFSFRSVTARSWEALAYLVVFGSLVAFSAYNWLLERYSPTLV"
BASE COUNT          313 a          412 c          437 g          397 t
ORIGIN      
        1 tgcatcagcc tagtcgctcg atgatttaga tttttctgcg atttttctct gccgtacgcg
       61 cgagcgttgc acgcggtcgc ggttgccgca ggtgcggtca ttgcaccagc ggcgcgtgtt
      121 ggctttggtc cgatcataaa aaatccactt acagccttca ttagggcaat acttcaggcg
      181 gtccggacga ttcatggcta gcatttctgc gaaggacgcg gcgatgcgcg aaagtatcca
      241 cggccaaccg gggcggagcg gaacaagctc ggactgcact ccattttggt gctggaacag
      301 ctttacgcgc acgggtacgt tcaaatacga attaagagca cttaaatcgc gctcatcgat
      361 gctctcaccg gcgataagtt tttcagccat gcgccgcagc agcgcccgta gcgcgatgag
      421 ctgcgcgaga ggaataggcc ggggcagagt ggaggctaga ttccaatgtt tgacgatgtg
      481 ggcggcccac gccgaatcct tcaagtgatc ggtgaccttg ccataaccgt tccattcgag
      541 ggagttggct aaatccagcc aggcaaactc cttaccgaag cccgccgcaa cgaaattgtc
      601 agcaccctgt ttcataatct tgattatggt aatgcgttaa attcatttga cccgttacac
      661 agaataggtt agtgttgttt gtgtaatgag tgtaacacga ataggccgtt acacaaatgt
      721 tggatgaaac caggtgagat gctatgagtg ccccgacgat gtccgcgaca atcacaaaat
      781 ccgccacgtt aaattctgat acctcccaga aggccgcggc ttccaaccgc ttgctgatca
      841 tcttttgctt tttcgcgatc tacgccatct ggggttccac ctacctggcc attcgctacg
      901 ccgtggaaac catacccccg ctgtatgcag cgggaatccg gcatctcacc tccgggacca
      961 tcttgctgct gtggtgcttc gcgaaaaaag tgcgaccaac cgcggcgcaa atccggactg
     1021 gcgtggtcat tgggattttg ttctttcttt tggggcacgg accactgcac tgggcggaga
     1081 cgagagtgcc ttccggcctc gcgtcactgt tggtggctac cgagcccatc tttgttttct
     1141 ttctcgcggc tctggcttca aagcgctggc aatttagctg gaaaatattc gctggcgtgt
     1201 tcctgggact ggccggcgtc ggcttgctgg tgggacgaac aacacttacg tccagtaagg
     1261 gaatgatagt tgcatcgctg gcgattttgt tcggcgcgct gtcatggtcg gcgggaatta
     1321 tttatgcgcg caagtcggat ctggccggtc atccgctgct tatgactgct ctatcttcgc
     1381 tttcgggggg aattctgctg ctcagcagcg caacgatatt tggtgaatgg cgaggctttt
     1441 cgtttcgttc cgttacggca cgatcctggg aagcgctggc atacctggtt gttttcggat
     1501 cgctggtggc gttcagcgcc tacaattggt tgttggaacg ctactcaccc acgctggtg
//
LOCUS       DATCBB010000829         1570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_187789, whole genome shotgun sequence.
ACCESSION   DATCBB010000829 DATCBB010000000
VERSION     DATCBB010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187789"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>1570
                     /locus_tag="VII37_09170"
     CDS             40..>1570
                     /locus_tag="VII37_09170"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069950.1"
                     /translation="MAIPHNAITVVTGVSGSGKSSLAFDTVYAEGQRRYVESLSAYAR
                     QFLERMEKPDVDEISGIAPAVAIRQKNSTRNPRSTVATATEIYDYLRLLFARCGQTFC
                     IKCGTEVRRDNPDEIATRVLTMTPGRRFYVLYEFRPGGMPPASPSADVTAAAAPSSRR
                     VTKKKVAQPSAESLRQSLIDLQKRGFNRLYQDGRMHEFSSPETLLDIDFSRPVFVVVD
                     RLAINPESRSRLVDSIEICYREGHGEALLEFVADAAGPAERLRFNERFECKKDGTLYQ
                     EPEPRLFSFNNPYGACPRCQGFGNTIDFDLNLVVPDSSKSLDEGAILPWTKPRYRNLL
                     QDAKKWARGRGIPTNVAWRHLSEEQRRLILEGDPESDYEGITGFFKYLERKKYKLHVR
                     VFLSRFRGYATCLECGGTRLRAEARAVRVAGRSITEVCKLTVKEARVFFSNMQLTQAQ
                     NTVADKILQEVRSRLQFLDEVGLDYLTLDRLTSTLSGGESQRIQLATSLGSHLVGALY
                     VL"
BASE COUNT          308 a          358 c          518 g          386 t
ORIGIN      
        1 gttcgcgggg cgcgcgttca caaccttaaa aacattactg tggcgattcc gcataacgcc
       61 attacggtgg ttactggcgt aagtgggtct ggtaagtctt cgctggcgtt tgacaccgtg
      121 tacgccgagg ggcagcggag atatgtggag tcgctttcgg cgtatgcgcg gcagtttctg
      181 gagcggatgg aaaagccgga tgtggatgag atttccggga ttgcgcctgc agtggcgatc
      241 cgacagaaga attccacgcg taatccgcgg tctacggtgg ctacggctac ggaaatttat
      301 gattatttgc ggctgctttt tgcgcggtgc ggtcagacgt tttgtataaa gtgtggcacg
      361 gaagttcgcc gggataatcc ggatgagatc gcgacgcgag ttttgactat gacgccgggg
      421 cggcgtttct atgtgcttta tgagtttcgt ccgggcggga tgccgccagc gtctccttcc
      481 gcggatgtta cggctgctgc tgcgccatcg tcacggcgcg tgacgaaaaa gaaagttgcg
      541 cagccttctg ctgagtcgtt gcggcaatca ttgattgatc tccagaagcg tgggttcaac
      601 cggttgtatc aagacggacg gatgcatgag ttttcttctc cggagacttt gctggatatt
      661 gatttttctc gacccgtatt cgtggtcgtt gatcggctgg cgattaatcc ggagtctcgc
      721 tcgcggctgg tggattcgat tgaaatttgc tatcgcgagg ggcatggcga ggcgctactg
      781 gaattcgtgg cggatgcggc gggtccggcg gagcggctgc gatttaacga gcgattcgaa
      841 tgcaagaagg acgggacgct gtatcaggag ccggagccgc ggcttttttc tttcaataat
      901 ccttacggag cttgtccacg atgccaggga tttggcaaca ccatcgattt tgatttgaat
      961 ctggtagtgc cggattcgag caaatcgctg gatgagggcg cgattttgcc ttggaccaaa
     1021 ccgcgctatc gcaatttgtt gcaagacgcg aagaaatggg cgcgcgggcg agggattccg
     1081 acgaatgtgg cgtggcggca ccttagcgaa gaacagcggc ggttgattct ggaaggcgac
     1141 ccggagagcg attacgaagg catcaccgga tttttcaaat atctggagcg caagaaatac
     1201 aagttgcacg tgcgggtgtt tctgagtcgt tttcgcggct atgcgacttg tctggaatgc
     1261 ggcgggaccc gactgcgcgc tgaggcgcgg gcggtgcgcg tggcgggacg gtcgattacc
     1321 gaggtttgca agctgaccgt caaagaagcg cgcgtatttt tctccaacat gcaactgacc
     1381 caagcgcaaa acaccgtggc ggataaaatt ctgcaggaag ttcgctcacg gctgcaattt
     1441 ctggatgaag tggggttgga ttacttgacg ctggatcggc ttacttccac gttgtcggga
     1501 ggcgaatcgc agcggattca gctggctact tcgctgggtt cgcatctggt gggggcgttg
     1561 tacgtgttgg
//
LOCUS       DATCBB010000830         1365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_91025, whole genome shotgun sequence.
ACCESSION   DATCBB010000830 DATCBB010000000
VERSION     DATCBB010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91025"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..522
                     /locus_tag="VII37_09175"
     CDS             115..522
                     /locus_tag="VII37_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5069951.1"
                     /translation="MSSASQPLTTTEQNKLLVIRWFDEVWNQGRRETIFELLPESGIL
                     HDGALQYRGPEEFARFYDSLRSQFSNFKITPIISLAEGNHACVHWNCSMMHAGTKKAL
                     QLTGTSVVRIENGQFAEAWQNWDAAHLQAQLSE"
     gene            862..>1365
                     /locus_tag="VII37_09180"
     CDS             862..>1365
                     /locus_tag="VII37_09180"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5069952.1"
                     /translation="MSTSHHNSNGAPQVPDGSSGINVVAGQSRGVTYWRVEGSLLELG
                     ALRPVLFFTWNSRSYAERWARRAGMAGMALVRPFTYAASRTFATRFLHTLLRGVSRDR
                     LDLLGEEYFQYVLKPQMRRDAVDKLVEALRSGERVVLVSQLLDHILKPLAKHFGVESI
                     IANRLDFR"
BASE COUNT          272 a          345 c          418 g          330 t
ORIGIN      
        1 cgcgattatc aggagctgtc gattcggatg cgacttagaa gagcgatgga cagtcgctag
       61 taaccggtcg cacagttcta tatactctgc ccgaagtctt tccaggagtt caatatgtct
      121 tccgcttccc aaccccttac cactacggaa cagaacaaac tgctggtgat tcgctggttt
      181 gacgaagtat ggaaccaggg ccgtcgtgag acgatcttcg aactcctgcc tgaatccggc
      241 attctccacg acggcgctct gcagtaccgc ggcccggagg aatttgcgcg cttctacgat
      301 tccctccgtt cgcaattctc gaacttcaaa attacgccga tcatttctct ggcagaaggt
      361 aatcacgcct gcgttcactg gaactgctca atgatgcacg ccggcacaaa aaaagcattg
      421 cagttaaccg gcacctcagt ggtgcgtatc gaaaacgggc agttcgccga agcctggcaa
      481 aactgggacg ctgcccacct gcaggcgcag ctgagcgaat gatcgaatgg tttctcctag
      541 gtgctggtga cacgattagt gccgaaccca gagacgaaaa gagaccgctc ttgcacgttg
      601 agtcgtgatt aaggcgcttg tcggaaactg agccgtcttg cgggaagccg acttggagtg
      661 ctacttttag gcggtatttt ggggcgccgg cggagttggg gctggcgggg cttggctgcg
      721 gctgacgacc gatccaagcc ggttatctat aattgaaact acaagatagt tttggccgtg
      781 cggtcgagtg cggatcgctg attaaatgta actgctgatt gcaactgttg gctgcgtttg
      841 acaagctgat tactcacctg catgtcgact tcacaccaca attcgaatgg tgcgcctcag
      901 gttcccgacg gttccagcgg catcaacgtt gtcgccgggc agtctagagg cgttacttac
      961 tggcgggttg aggggtcctt gctggagctt ggcgcgctgc gtcccgtttt gttttttacg
     1021 tggaattcgc ggagttatgc ggagcggtgg gctcggcgag ctgggatggc gggcatggcg
     1081 ttggtgcgtc cgttcacgta tgcggccagc cgcacgtttg cgacgcggtt tttgcatacg
     1141 ctgctgcgcg gagtgagccg cgaccggttg gatttgttgg gcgaggaata ttttcagtat
     1201 gtgctgaagc cgcagatgcg gcgcgacgcg gtggataagc tagtggaggc ccttcgttcc
     1261 ggcgagcgcg tagtgctggt cagccagttg ctggaccata tattgaagcc gttggcgaaa
     1321 cattttgggg tcgagtcgat catcgcgaat cggctggatt ttcgc
//
LOCUS       DATCBB010000831         1461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_123934, whole genome shotgun sequence.
ACCESSION   DATCBB010000831 DATCBB010000000
VERSION     DATCBB010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123934"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VII37_09185"
     CDS             complement(<1..185)
                     /locus_tag="VII37_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069953.1"
                     /translation="MAKSRREFLTQGSLGLMGTVVVARDDLLATAQPGAQEPTPGAPP
                     AFGTGPAVGPEVSTATFA"
     gene            complement(345..854)
                     /locus_tag="VII37_09190"
     CDS             complement(345..854)
                     /locus_tag="VII37_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5069954.1"
                     /translation="MGIFDKMFGSGAQGAQDANSLKRFEELKQKYQSVLRSIEQQQVQ
                     LQNLHVQDNKLFIKGTAPSEQVKNKIWDEIKLVNPNADDITADFTAAQGASQAAAAGA
                     SASGPQGTGGGQRYTVKGGDSLSKISQQFYGNAHDYMKIFYANKDKVSDPNKIQPGME
                     LTIPATEGK"
     gene            1032..>1461
                     /locus_tag="VII37_09195"
     CDS             1032..>1461
                     /locus_tag="VII37_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5069955.1"
                     /translation="MAARIYLDYNATTPVDPAVLEAMLPFFSGEFGNAASIHTTGQKA
                     RAAVETAREQVAALINANPQEIIFTSGGTESDNHAIFGMFNPTNYAQANLVSTVIEHE
                     AVLNTCQALAARGLTVGYLAANREGQIDPQHLRDLLHEMPD"
BASE COUNT          325 a          478 c          317 g          341 t
ORIGIN      
        1 gcaaatgtcg cggtcgacac ctccggaccg accgcagggc ccgtcccaaa tgcaggcggc
       61 gctcccggcg tgggttcctg ggcgccaggc tgcgcagtag ccagcaagtc gtcgcgcgct
      121 acaaccactg tccccatcag acccagcgaa ccctgcgtca aaaactccct gcgcgattta
      181 gccatcgttc ctccgcaagc attgtagacc gagtctttcc tcgatcacgc cgcagcggtc
      241 aatttcccaa atacctaaat acgaaccgtt atgctggcca aaaaaaaagg gccggaaata
      301 tccggcccgg cccggccctg ctaaaaataa aataccaggt gtatctactt tccctccgtt
      361 gcgggaatcg tcaattccat cccaggttga atcttgttgg gatcgctgac tttgtccttg
      421 ttggcataaa aaatcttcat gtagtcatgc gcattgccat aaaactgctg gctgattttc
      481 gacaggctat cacccccctt cactgtgtag cgttgtccgc cgcccgtccc ttgtggcccc
      541 gaggcagacg cgcccgctgc agccgcctgt gacgcgcctt gcgccgccgt aaaatccgcg
      601 gtgatgtcgt cggcgttagg attgaccaac ttaatttcgt cccaaatttt atttttaact
      661 tgctccgacg gagcagtccc ctttatgaac aacttattgt cttgcacatg cagattttgc
      721 agctgtactt gttgctgctc aatgctgcgc aacacggact gatatttctg tttcaactct
      781 tcgaaccgct tcagcgaatt tgcatcttga gcgccttgcg cgccactgcc aaacatcttg
      841 tcgaatattc ccattgattc ctcctcagat tgagctggat gcatcaacct aaccacgcgc
      901 gaaccatccc cgcaatacac ccatccgtgt acgcatgctc agaccggcag aatttaactg
      961 caacgtctcg cagtcttacc accaagcgcg taattttttc ctcgccccac tcctgttatt
     1021 ctcaagctgt catggccgcc cgcatttatc tcgattacaa cgccaccact ccagtcgacc
     1081 ctgccgtcct cgaggccatg cttccgttct tttccggcga attcggcaac gccgcatcca
     1141 tccacaccac cggacaaaaa gcccgcgccg ccgtcgaaac cgcgcgcgag caagtcgccg
     1201 ctttaattaa cgcgaatcct caggaaatta ttttcacaag tggtggtaca gagtccgaca
     1261 atcacgccat cttcggcatg tttaatccca cgaattacgc acaagcaaat ctcgtctcca
     1321 cggtcatcga acatgaagct gtactcaaca cttgccaggc gctagccgcc cgcggtctta
     1381 ccgtcggcta tctcgctgcc aaccgcgagg gacaaatcga tccgcaacat ctgcgcgacc
     1441 ttcttcacga aatgccagac a
//
LOCUS       DATCBB010000832         1479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_91167, whole genome shotgun sequence.
ACCESSION   DATCBB010000832 DATCBB010000000
VERSION     DATCBB010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91167"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VII37_09200"
     CDS             <1..869
                     /locus_tag="VII37_09200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5069956.1"
                     /translation="EKAQIHSLRTGCEFVIATPGRLQDFITRKFADLRHVKILVLDEA
                     DRMLDMGFLPAIRRILQILPQRRQTLCYSATMEQSVASLVSDYTRDPVRVALGSTLKP
                     AESVQLEAYEVRPSEKLDVLRQLLYAEKGQTLIFTRTKRGTQRLAKDLERDGFSVAMI
                     HGDRTQSQRNGALSGFQEGRYQVLVATDVASRGLHVDDVAHVINYDLPKMAEDFIHRV
                     GRTGRAGLQGRASTLFSAAENMDLRSIERTLKLRIERKQVKNSTSEQPKRFIANTLAS
                     RTLTKLPGEVFV"
     gene            complement(977..1231)
                     /locus_tag="VII37_09205"
     CDS             complement(977..1231)
                     /locus_tag="VII37_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069957.1"
                     /translation="MPRGRSTFTKRQKEQARQQKQRDKNEKKTQRKQDQPEGVVVNEE
                     EELRQMREHAAEQAALFGIGSDGDEPEPVDLIPPHKQVSR"
BASE COUNT          318 a          409 c          388 g          364 t
ORIGIN      
        1 ccgaaaaggc ccagattcac agcctgcgta cgggatgcga atttgtaatc gcgactccgg
       61 gccgcttgca ggatttcatc acccgtaagt tcgccgacct gcgtcatgta aaaatcctcg
      121 tccttgacga agctgaccgc atgctggaca tgggctttct tcctgcgatc cggcgcattc
      181 tgcagatttt gccgcagcgg cgccagaccc tctgctactc cgcgactatg gagcaatcgg
      241 tggcgtcgtt agtgagcgat tacacgcgtg atccggttcg cgtggcgctg gggtcaactt
      301 tgaaaccagc ggagagcgta cagctcgaag cttatgaagt gcgcccatcg gagaagctcg
      361 acgtgttgcg ccagttgctc tacgcggaaa aagggcagac gctgatcttt acccgcacca
      421 agcgcggcac ccaaaggctt gccaaggatc tggaacgcga tggtttttcg gtcgctatga
      481 ttcacggcga tcgcacgcag tcgcagcgta atggcgccct gagcgggttt caggaaggcc
      541 gatatcaagt gctggtcgcg accgatgtgg catcgcgcgg cttgcacgtt gacgatgtag
      601 ctcacgtcat caactacgac ttgccgaaga tggcggaaga cttcatccac cgcgtaggcc
      661 gcaccggacg cgctggcttg cagggacgcg cctcgacgtt attttcggca gccgaaaata
      721 tggatctgcg aagtatcgag cgcaccttga aactgcgcat cgaacgcaaa caggttaaga
      781 attccaccag cgagcagcca aaaaggttca tcgcaaatac cctggcatcc cgcaccttaa
      841 ccaaattgcc cggcgaagta tttgtctaga aatccagaaa gtggagcgac gggcgtttct
      901 cgcccgtcct tccattaaat agattttgag ttggctaaaa ctggcatcgc cggcgcgcaa
      961 gctccggcga ttttaactag cggcttactt gcttgtgcgg cgggattaaa tcaaccggct
     1021 ctggttcgtc gccatcgctg ccgatgccga acaatgcagc ctgttccgcg gcatgctcgc
     1081 gcatctggcg caattcttct tcctcattta ccacaacgcc ttcgggttga tcctgcttcc
     1141 gttgcgtttt cttctcgttt ttatcgcgct gtttttgctg gcgcgcctgc tctttctgac
     1201 gtttggtaaa agttgatcgg cctcgtggca ttcaaatctc cccgtaatcg tttaattaat
     1261 attaatggct agctggaata attccttcgg ttcgtgttta gtccggcgtt cggcccggtt
     1321 acgcaatcaa atcgtgcggc tttagcagga ataatccaca aaattccaaa atcgcgtttg
     1381 attgtctaca tagtataacc tcgattccat tgctgaggca cattgagacg atggcatctt
     1441 gggattgcca ttacttgctt ccccatatac aaccgagta
//
LOCUS       DATCBB010000833         1510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_252844, whole genome shotgun sequence.
ACCESSION   DATCBB010000833 DATCBB010000000
VERSION     DATCBB010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252844"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..353
                     /gene="rpsK"
                     /locus_tag="VII37_09210"
     CDS             6..353
                     /gene="rpsK"
                     /locus_tag="VII37_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY5069958.1"
                     /translation="MVPVGVVHVQASFNNTIITITDPMGNVLSWSSSGSLGFRGSRKG
                     TPFAAQQASLTAANKAKESGLRTVNVEVSGPGAGRESAVRALSTAGLEVRIIKDLTPI
                     PHNGCRPPKKRRV"
     gene            431..1063
                     /gene="rpsD"
                     /locus_tag="VII37_09215"
     CDS             431..1063
                     /gene="rpsD"
                     /locus_tag="VII37_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY5069959.1"
                     /translation="MARYIGPVCRQCRREGMKLFLKGERCHTEKCAIEKRNFIPGQHG
                     KDRKPKIVGYGLQLREKQKARRYYQLLEGQFRNLFEKASGMKGITGDNMLNMLERRLD
                     NVIYRMGFGTSRAQSRQLVRHGHVQVNGRRVDIPSFQVKPNDVIEIQEASKKNPAVLH
                     ATEATAHSPSPNWLEVDRENLRARVLGQPKREDLVQIQLNEQLIVELYSK"
     gene            1199..>1510
                     /locus_tag="VII37_09220"
     CDS             1199..>1510
                     /locus_tag="VII37_09220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013189.4"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY5069960.1"
                     /translation="MFIAQPFERGFGSTIGTGLRRVLLSSIEGAAITAVRIEGVEHEF
                     SPIPGVVEDATDIILNLKQIPFKIMGDGVKTVRLTVDQHGDVRSGQIETDADVEVLDR
                     DV"
BASE COUNT          352 a          400 c          479 g          279 t
ORIGIN      
        1 agaagatggt gcccgtgggc gtggtccacg tgcaggcgtc tttcaacaac accatcatca
       61 ccatcacgga ccccatgggg aacgtgctgt cgtggtcgag ctcgggttcg ctggggttcc
      121 ggggatcgcg caagggtaca cccttcgccg cgcagcaggc ttcgctgacc gcggccaaca
      181 aggccaagga atcgggcttg cgaactgtga acgtggaagt gagcggtccg ggagcgggcc
      241 gcgagtcggc ggtgcgcgcg ctgtcgacgg ccggattgga agtgagaatc atcaaggatc
      301 ttacgcccat tccgcataac ggctgccgtc ctccgaagaa gcgcagagtt tgatttaaag
      361 aagaagtaaa gcaggacgaa gacccacggg gttgtaaact gcacctgact gaagtttagg
      421 gaggtttgat ttggcacgat atattggtcc cgtgtgccgg caatgccggc gcgagggcat
      481 gaagctgttc ctcaaaggcg agcgctgcca caccgagaag tgcgccatcg aaaagcgcaa
      541 tttcattccc ggccagcacg gcaaggatcg taagccgaag attgtcggct acggtttgca
      601 gcttcgcgaa aagcagaagg cgcgccgcta ttatcagttg ctcgaaggcc agttccgcaa
      661 cctgttcgag aaggccagcg ggatgaaggg tatcaccggc gacaacatgt tgaacatgtt
      721 ggaacgccgt ctggacaacg taatttaccg catgggcttc ggcacttcgc gcgcgcagag
      781 ccgccagttg gtgcgtcatg gccacgtgca ggtgaacgga cgccgcgtgg atattccgtc
      841 gttccaggtg aagcctaacg acgtgattga gattcaggaa gccagcaaga agaacccggc
      901 ggtgttgcac gccaccgaag ccaccgcgca ttcgcccagt cccaactggc tggaagtgga
      961 tcgcgagaat ctgcgggcgc gcgttttggg acaacccaag cgcgaagacc tggtgcagat
     1021 tcagttgaac gagcagctca ttgtggaatt gtattcaaaa taggtgagtt ggattcgaag
     1081 taggcaaatt gtattcgagt aggcgttcgg aaaacagagg agccacagaa tgttcaaagg
     1141 atttcaaaaa ccaaagaggt tagttgcgaa taccgaaacg cttaccgagc gttacggcat
     1201 gttcatcgcg cagccgttcg agcgcggatt cggctcgacc atcggcaccg ggctgcggcg
     1261 cgtgctgctg agctcgattg aaggcgcggc catcaccgcg gttcgcatcg aaggcgtgga
     1321 acacgaattc agcccgatcc cgggcgtagt cgaggacgcc accgatatca ttttgaacct
     1381 gaagcagatc cctttcaaaa tcatgggcga cggcgtgaaa accgttcgcc tgaccgtgga
     1441 ccagcacggc gacgtgcgca gcgggcagat cgagaccgac gccgatgtcg aagtgttgga
     1501 ccgcgacgtc
//
LOCUS       DATCBB010000834         1004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_416410, whole genome shotgun sequence.
ACCESSION   DATCBB010000834 DATCBB010000000
VERSION     DATCBB010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416410"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..>1004
                     /locus_tag="VII37_09225"
     CDS             180..>1004
                     /locus_tag="VII37_09225"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY5069961.1"
                     /translation="MLSRVAESLYWMSRYLERAQHTARLIDVDLQLRLDQSLESSTGR
                     WLRLVEALHVPALLAGNVDATRLAHTLTFNRTNPSSIVSCIASARENLRHVREQSSSD
                     MWEQLNRLYLQMIASPAEDEWVLRSHVLFRAVQDGAHLFQGITDSTMSHGEGWQYIQL
                     GRFVERTDAVAALVATHFSRLSHPANQPVEGADYLDWVGLLKCCAAFEAYCKAYSAEL
                     RPIRVAEFLLLNSEFPHSIRFSVESVHAGLGRIGELTERKVTQPARIAGRMRAALSF"
BASE COUNT          211 a          249 c          311 g          233 t
ORIGIN      
        1 accctctcgc aaagaacgcg agaaggatgg ggcgcccggg caggtcagcg ccacacgagg
       61 ttgggcgtta ccaggttgag agcgcgggca ctactcgcga gatccttcgc aggctcagga
      121 tgacgtcaaa aaattaaggg agcgcatttc gcagtaagtt tattcagcac aaattattta
      181 tgctttcacg agtggcagag agtctttact ggatgagccg ttatctcgag cgcgcgcagc
      241 atacggcgcg cctgattgac gtggacctgc aactgcgtct cgatcaatcg ctcgaatcta
      301 gcacgggacg gtggttgcgg ctggtagagg ccttgcacgt cccggctctg cttgcaggca
      361 acgtagacgc gacgcgtctg gcacacacgc tgactttcaa ccggacgaat ccctcgtcca
      421 ttgtttcttg tatcgcttcg gcaagagaaa atcttcgtca tgtgcgggaa cagtcgagtt
      481 ccgatatgtg ggaacaactg aaccggctgt atctgcaaat gattgcgagt cctgcggaag
      541 acgaatgggt tttgcgatcc catgttctgt ttcgcgcggt gcaggatggc gcgcaccttt
      601 ttcagggcat cacggattcg acaatgtctc atggcgaagg atggcagtac attcagctgg
      661 gacgatttgt agaacgaacg gatgcggtcg cagcgctggt ggctacgcat tttagccgcc
      721 tatcgcatcc ggcaaatcag cccgttgaag gggcggatta tctggattgg gtgggattgt
      781 tgaagtgctg cgcggcattt gaggcttatt gcaaagccta ttccgccgag cttcggccga
      841 ttcgcgtggc ggaatttctg ctgctgaatt ctgagtttcc acattcgata cgcttctcag
      901 tcgaaagcgt acatgcgggg ctgggccgta tcggcgaact tacggaacgc aaggtgacgc
      961 agccggcgcg aattgcgggg cggatgaggg cagctttgag cttt
//
LOCUS       DATCBB010000835         1149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_59252, whole genome shotgun sequence.
ACCESSION   DATCBB010000835 DATCBB010000000
VERSION     DATCBB010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59252"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VII37_09230"
     CDS             complement(<1..719)
                     /locus_tag="VII37_09230"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5069962.1"
                     /translation="MSLLASAISQFEANLAPPEFANKIRQLHRAQVLSALQMTAELFR
                     LVDRFRDARLDIVLVKGPVLAVRAYGDTGARQYGDLDFLVRQKDILRATELMIAAGYE
                     PEIPAEAVSPEKIPGQYVFVRIASPLLVELHTERTMRYFPRGLPIEEFFARRQPVSVD
                     GHQIPALAIEDELILICIHGAKHLWERLSLVADVAAFAARQTSLDWERSLATARSVGA
                     ERMMNTGLVLPSNLLGATLPQ"
     gene            923..>1149
                     /locus_tag="VII37_09235"
     CDS             923..>1149
                     /locus_tag="VII37_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069963.1"
                     /translation="MYVTAILVMTQNPCTPQHGPPQRVRTQMIAAQIKKAGPVVVRNR
                     PRNTKLSILLLAHIHFRVDARLDLLQSFKTKL"
BASE COUNT          279 a          314 c          317 g          239 t
ORIGIN      
        1 ttttgcggca atgtagcgcc gagcaaattg cttggtaaaa ccaggccagt attcatcatg
       61 cgttccgcac cgacacttcg cgcggtggca aggctgcgct cccaatctag agaagtttgg
      121 cgggctgcga acgcggcaac atcagcaacc agcgacaaac gctcccaaag atgtttggcg
      181 ccgtgaatgc aaatcaggat cagctcatcc tctatggcga gagcggggat ctggtgccca
      241 tctacgctta caggttgccg ccgtgcaaaa aattcttcta tcggaagccc gcgaggaaag
      301 taacgcatgg tgcgctcggt atgcagttcg acaaggagcg gcgatgcgat tcgaacgaaa
      361 acgtattgtc caggaatttt ttcggggcta actgcctccg caggtatttc cggctcgtaa
      421 ccagcggcaa tcatcagctc ggtagcgcga agaatatctt tttggcggac gagaaagtcg
      481 agatcgccat attgtctcgc gccagtatcg ccatacgcgc gtacagccag gaccggacct
      541 ttaaccaaca cgatatccaa tctagcgtcg cgaaaccgat caaccagtcg aaatagttcc
      601 gcggtcattt gcaacgcgga cagcacttgg gcgcggtgga gttggcggat cttgttagca
      661 aactcaggtg gggctaggtt ggcttcgaac tgggaaattg cagaggcgag cagcgacata
      721 actccatgcc cttcggccaa cgtaaatagc tcgggccatg acgcctcgcg aagcgctgcg
      781 gaaagtgtcg ataggtcggc gtgaggagag gcgcactgca gcaaagcggc ccattctggt
      841 gaaccaccgc ttgacagatt gttcctaatc gattttgtgg gagtcgattc caaaacagtg
      901 aactccagcg ccccgcggcc tagtgtacgt gacagctatt ctggtcatga cacaaaatcc
      961 ttgcacgcca cagcacggcc ctccgcagcg agtccgcaca cagatgattg cggcgcaaat
     1021 aaaaaaggcc ggtcccgttg tggtgcggaa ccggcccaga aacaccaaac tatcaattct
     1081 tcttcttgcg cacattcact ttcgtgtcga tgcccggctg gatttgttgc agagcttcaa
     1141 gaccaagct
//
LOCUS       DATCBB010000836         1557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_382931, whole genome shotgun sequence.
ACCESSION   DATCBB010000836 DATCBB010000000
VERSION     DATCBB010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382931"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VII37_09240"
     CDS             <1..1316
                     /locus_tag="VII37_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY5069964.1"
                     /translation="RPGQRFERRPSGPGGPRHDRGHQGGHGHHSGSSRRPQLISEMLK
                     AGQDVVIQIAKEPLGKKGARITSHVALPGRFLVYMPTIDHIGVSRKIASADNRSRLRR
                     LVGEARGSYPGGFIVRTAAGGATDEEIRADIEFLGTTWNEIKERSEQRKAPSLLHRDL
                     NLVERILRDYVSDDFTAIWIDSEEEFSKVVEFVSRFQPKLVSRVKLYTKETPIFEEFG
                     IQHELDKALRAKVWLKSGGYIVINHTEALVAIDVNTGKFVGKGSTRLEDTIVKTNLEA
                     VKEIVRQIRLRDLGGIIVVDFIDMEERRNRDKVLFALQQALEEDKAPSKALSFNEFGL
                     VCITRKRTKQALERVLCQPCPYCTGSGMVKSIPTLCYEIQAEARKMAMAGHEGPNLTL
                     RVHPEIAKALKTRESLLMDELEQTSHKHIIIQSDATLHWEQYDIY"
     gene            1326..>1557
                     /locus_tag="VII37_09245"
     CDS             1326..>1557
                     /locus_tag="VII37_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069965.1"
                     /translation="MNALQTLLKTDPAQVDSLSSEQILALCGNGKLTDSSLCSVDFRE
                     YLQVAKSESLIKCLRASLEGRFEAGPFILQDVI"
BASE COUNT          376 a          494 c          409 g          278 t
ORIGIN      
        1 gtcgccccgg ccagcgcttc gagcgccgtc ccagcggacc aggcggtccg cgtcatgacc
       61 gcgggcatca ggggggccac ggccatcact ccggctcttc acgccgcccg caactcattt
      121 ccgaaatgct caaggctggt caggatgtag tcatccagat cgccaaggaa ccgctcggca
      181 aaaagggcgc gcgcatcacc agtcacgtcg ctttgcccgg acgttttctc gtgtacatgc
      241 cgaccattga tcacatcggc gtctcgcgca aaatcgccag cgccgataat cgctcgcgtc
      301 tgcgccggct cgtcggcgaa gcgcgcggca gttatcccgg cggcttcatc gtacgcaccg
      361 cggccggcgg cgccaccgac gaagaaattc gcgccgacat cgaattcctc ggcaccacct
      421 ggaatgaaat caaggagcgc agcgagcaac gcaaagctcc ctctctcctc catcgcgatc
      481 tgaatctcgt ggaacgcatc ctgcgcgatt acgtcagcga cgatttcacc gccatctgga
      541 tcgacagcga agaagaattc agcaaagtcg tcgaattcgt cagccgcttc cagcccaagc
      601 tggtcagccg cgtcaagctg tacaccaagg aaacgcccat cttcgaagag ttcggcatcc
      661 agcacgaact cgacaaagcg ctgcgcgcca aagtgtggct caagtccggt ggctacatcg
      721 tcatcaacca caccgaagcg ctggtggcca tcgacgtcaa caccggcaaa ttcgtcggca
      781 aaggttccac gcgcctcgaa gacaccatcg tcaaaaccaa tctggaagcg gtgaaggaaa
      841 tcgtgcgcca gattcgcttg cgcgatctgg gcggcatcat cgtggtcgat tttatcgaca
      901 tggaagagcg ccgcaaccgc gacaaagttt tgttcgcgct gcagcaagcc ctcgaagaag
      961 ataaagcgcc gtcaaaagcg ctgagcttca acgagtttgg cctggtgtgc atcacccgca
     1021 agcgcaccaa gcaggctctc gagcgcgtgc tctgccagcc gtgcccgtat tgcaccggct
     1081 ccggcatggt caaatccatt cctacgctgt gctacgaaat ccaggccgaa gcccgcaaaa
     1141 tggccatggc cggccacgaa ggccccaacc tgaccctgcg cgtgcatccc gaaatcgcca
     1201 aagcgctaaa aacccgtgag tccctgctca tggacgaact ggagcaaacc tcgcacaaac
     1261 acatcatcat ccaatccgac gccaccctgc actgggagca atacgatatc tactaagcgg
     1321 tcgaaatgaa cgcactacaa acattgctca agacggatcc tgcgcaggta gattcgctat
     1381 cgagcgagca aatcctagcg ctctgcggaa atgggaaact gacagacagc tctctatgct
     1441 cagtagattt tcgcgaatat ctacaagtgg caaaaagcga aagcctgatc aagtgccttc
     1501 gagcatcgct tgaggggaga ttcgaggctg gtccgtttat tctgcaagat gtaatta
//
LOCUS       DATCBB010000837         1251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_222306, whole genome shotgun sequence.
ACCESSION   DATCBB010000837 DATCBB010000000
VERSION     DATCBB010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222306"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1251
                     /locus_tag="VII37_09250"
     CDS             <1..>1251
                     /locus_tag="VII37_09250"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HEY5069966.1"
                     /translation="VNDAGEEVAERTEGFLWFRGPSATTGYYQNKAATEALLPRGPGD
                     GNGEFAWVNSGDRAYIADGELYVTGRVKDIIIKGGRNLYPHEVEELAARVEGIRKGCI
                     VAFGIKDQASGTEKMVVVAESRETNGARRLEMTAKITEVVARGLGLPPDRVELLPLGS
                     IPKTSSGKLRREETKQLYLAGTLSENKAPAWLQIARLGAGGAASATGRAIGNGVRRGL
                     EFLYGIYFVVVFTLWIVPTWMIVQLYTDHCAAGKFTNRALKILFFLIGCKVRVTGKEY
                     METPGAKIYASNHTSYFDVVALMMGLGVPYRFVAKMEVTGMPFIGTFMRQMGHLSFDR
                     SDAESRLHQAAEMEDFLRKGESVFVFPEGTFTRDPGVRQFQLGAFKAAVETGAPIIPV
                     SLRGTREFLPDGSFLPRPSDVTITL"
BASE COUNT          268 a          246 c          452 g          285 t
ORIGIN      
        1 gtgaatgacg cgggggagga agtcgcggag aggacggagg ggtttctgtg gtttcgcggg
       61 ccttcggcga ctaccggtta ctaccagaat aaagcggcga cggaggcgtt gttgccgcgc
      121 gggcctgggg atgggaatgg agaattcgcg tgggtcaatt cgggtgatcg ggcgtacata
      181 gccgatggcg agctgtacgt caccggccgc gtgaaggaca tcattatcaa aggcggacga
      241 aatttgtatc cgcatgaagt ggaggagctg gcggctcgcg tcgaagggat tcgcaagggc
      301 tgtattgtgg cgtttggaat taaggatcaa gcgagtggaa cggaaaaaat ggtggtggtg
      361 gcggagtcaa gagaaacgaa tggtgcgcgg cgtttagaaa tgaccgcgaa gattacggaa
      421 gtagtggcgc gaggtttggg gctgccgccg gatcgcgtgg aactgcttcc actgggaagc
      481 attccgaaaa cgtcgagcgg gaaattgcgg agagaagaga ccaagcaact gtatctcgcg
      541 ggaacattgt cggagaacaa agcgccggcg tggctgcaaa ttgcacggct tggggcgggc
      601 ggtgctgcat cggctacagg ccgagcgatt gggaatggcg tgaggcgtgg gctggaattt
      661 ctgtacggga tttattttgt ggtggtgttc acgctatgga ttgtgccgac gtggatgatc
      721 gtgcagctgt acacggatca ttgtgcggcg ggcaaattta cgaacagggc tttgaagatt
      781 ttgtttttcc tgattggctg caaagtgcgt gtgaccggaa aagaatatat ggagacgccg
      841 ggagcgaaaa tttacgcgtc gaatcacacg agttactttg atgttgtggc gctgatgatg
      901 gggctgggag ttccctatcg ctttgtggcc aagatggaag tgaccggaat gccgtttatt
      961 gggacgttta tgcggcagat ggggcatttg tcgtttgaca ggtcggacgc ggaatcgcga
     1021 ttgcatcagg ccgcggagat ggaagatttt ctgcgcaaag gcgaatccgt ttttgttttt
     1081 ccggagggca catttacgcg cgatccggga gtgcggcaat ttcagttggg agctttcaaa
     1141 gcggcggtgg agacgggggc cccgattatt ccggtgtcac ttagagggac acgggaattt
     1201 ttaccggatg ggtcgttctt gccgcggccc tcggacgtga cgattacctt g
//
LOCUS       DATCBB010000838         1018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_416447, whole genome shotgun sequence.
ACCESSION   DATCBB010000838 DATCBB010000000
VERSION     DATCBB010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416447"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VII37_09255"
     CDS             <1..382
                     /locus_tag="VII37_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY5069967.1"
                     /translation="RRAAGLTETVARASAGALAHLPVARVTNLSRAMEEMKEAGYWLV
                     GLDEAAEKGHTEVDFKGPVGIVLGGEGKGLHALVRERCDFLVSIPTTGPVRTLNVSVA
                     AGVVLFEVVRQRGQFRKATTDEHR"
     gene            complement(532..>1018)
                     /locus_tag="VII37_09260"
     CDS             complement(532..>1018)
                     /locus_tag="VII37_09260"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY5069968.1"
                     /translation="DYPKPGILFYDLTTLLQNPKAFHGLVDELCDHYTGQKVDAVVGI
                     EARGFILAPALAYRLNAGFVPVRKPKKLPSKAVSVTYQLEYGTDTLEIHEDAIKPGQS
                     VLISDDLLATGGTAAATVELVRKLGGKVVGASFAVELTFLNGRSRLPGIDVFTMIQYD
                     K"
BASE COUNT          212 a          272 c          303 g          231 t
ORIGIN      
        1 acgacgcgcc gccgggctta cggaaaccgt ggcgcgcgca tccgctgggg cgctcgcgca
       61 cttgccggtg gcgcgtgtca cgaatctttc tcgcgcgatg gaggaaatga aggaagcggg
      121 ctattggctt gtggggctcg atgaggccgc cgaaaaaggc cataccgaag tggatttcaa
      181 aggtccggtg ggaatcgtcc tgggcggcga aggcaaggga ttgcacgctt tggtgcgcga
      241 gcgctgcgat tttcttgttt caattccgac cacggggccg gtgcgtactc taaacgtttc
      301 agtcgccgcg ggggttgtcc tcttcgaggt ggttcgacag cgcggccagt tccggaaagc
      361 aaccacagat gaacacagat aaacactgat aaaagcaaaa caaggaaaat gaagggacgg
      421 caaaaaaccg attgagatgt cgtgccgctt ttgctctagt tccgcaatca gaatgttttt
      481 tctttatccg tgtttatctg tgttcatccg tggttccact gctttttctt tctacttgtc
      541 gtactggatc atcgtaaaga cgtcgattcc cggcagtctt gagcggccgt tcaagaacgt
      601 aagttcgacg gcgaaggatg cgccgaccac ttttccgccc agcttgcgca ccaattccac
      661 ggtggccgcg gcggtgccgc cggtggcgag caagtcatcg ctgatgagca cgctctggcc
      721 gggcttgatc gcgtcctcgt ggatttcgag cgtgtccgtg ccgtattcca gctgataggt
      781 aacggatacc gctttcgacg gcagtttttt gggtttccga acgggaacaa accccgcatt
      841 aagccggtac gcaagcgccg gcgccaaaat aaatccgcgc gcctcgatgc ccacgacggc
      901 gtctactttt tgccccgtat agtggtcgca cagctcatcg acaaggccgt ggaatgcctt
      961 gggattctgc agcagggttg taagatcgta aaaaagaatc cccggcttcg gataatcg
//
LOCUS       DATCBB010000839         1134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_318286, whole genome shotgun sequence.
ACCESSION   DATCBB010000839 DATCBB010000000
VERSION     DATCBB010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318286"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VII37_09265"
     CDS             <1..490
                     /locus_tag="VII37_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5069969.1"
                     /translation="QLLMSPASTTQIIVAKLFPLFVLLCMMGTMALALMRIVFGIPFR
                     GSTLLVVSAAALCLLCGISIGTFIATFTRSARQAQLTLFFVNPPLTSLSGAFTPVEAM
                     PKWLQPVAQFNPIQHFGQISRGTMLKGSGMETLWPNFLALLAFTVVLVSLSIWRFRKQ
                     LS"
     gene            511..>1134
                     /locus_tag="VII37_09270"
     CDS             511..>1134
                     /locus_tag="VII37_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069970.1"
                     /translation="MYTKSKARFFSIYHRARRGAELALLGALLYAAPALGQTAPGAAQ
                     TQTTQAVQLPLSGRTAQTNGTVKTSEAPAPSTTATVNTLNPNVQVQGAYTGSTPGVAK
                     MPFNGRLGLREAIQRGLAYNLGQTGATEALRQAQGQNKVALSSLLPNLNGTINENVQT
                     TDLRAQGFRFAFKGFTIPTVIGPFNYIDMRAHLSQSVLDLTAINNYRA"
BASE COUNT          248 a          332 c          311 g          243 t
ORIGIN      
        1 acagttgctg atgtcgccgg cttccacgac acaaatcatc gtcgccaagc tttttccact
       61 gtttgtactg ctgtgcatga tgggcaccat ggcgcttgcg ctgatgcgca tcgtgtttgg
      121 aattccgttt cgaggaagca ctcttctggt cgtgtcggct gccgccttgt gtctgttgtg
      181 cgggatcagc atcggaacgt ttattgccac gtttacaagg tcggcgaggc aagcacagct
      241 aactctgttt ttcgtgaatc cgccgctcac ttccctttcg ggcgcgttta cccccgtgga
      301 agccatgccg aaatggctgc agcccgtcgc acagttcaat cccattcagc acttcggcca
      361 gatttctcgc ggcacgatgc taaagggcag cggaatggaa acgctgtggc caaattttct
      421 cgcccttctg gcgtttaccg tcgtgctcgt ttcattgagc atctggcgat tccgaaaaca
      481 actcagttag gcccagcgca gaggagcctc atgtacacaa aaagtaaagc tagatttttc
      541 tcaatttatc accgcgcgcg cagaggcgca gagttagcgc tgctgggagc attgctatat
      601 gccgccccgg ccctggggca aactgctcca ggggccgcgc aaacgcaaac cacgcaggcg
      661 gtacagcttc cgctttccgg ccgaacggcc cagacgaatg gcacagtgaa aacttcggag
      721 gcgcctgcgc cgagcactac cgcgacggta aacacgctaa acccaaacgt gcaagtgcag
      781 ggcgcatata cggggagcac gccgggcgtg gcgaagatgc ccttcaacgg gcggctcgga
      841 ttgagggaag cgattcagcg cgggcttgcg tacaacctcg gtcagaccgg cgccacggag
      901 gcactacggc aagcgcaagg acagaataag gtggcgctca gctctttgct tccgaatcta
      961 aacggcacaa tcaacgagaa cgtgcaaact accgatcttc gcgcacaggg cttccgcttc
     1021 gcgttcaaag gattcacgat tcccaccgtg attggaccat tcaactacat agatatgcgc
     1081 gcacatcttt cgcagtcggt tctggatctg acggcgatca acaactaccg tgct
//
LOCUS       DATCBB010000840         1011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_350928, whole genome shotgun sequence.
ACCESSION   DATCBB010000840 DATCBB010000000
VERSION     DATCBB010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350928"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="cheB"
                     /locus_tag="VII37_09275"
     CDS             complement(<1..812)
                     /gene="cheB"
                     /locus_tag="VII37_09275"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEY5069971.1"
                     /translation="MSERIKVLVVDDSALMRKLIPAILTRDAAIEVVGTAMDGAFALK
                     KIEELRPDVVTLDLEMPRMDGMETLRLIMKSAPVPVILFSTHSKEGAYSTFKALALGA
                     VDFVAKPQDAAVGHLDAIADQLVEKIKVAKRASTHRLPPAVINDTPVGLKKRSGIAVP
                     PNRIIAIGISTGGPNALQFMLSQLPADFPSSILIVQHMPEGFTEMFARRLDECSALDI
                     HEARSGDLLLAGRVLLCPGNRHMMVRRMPRGDMVVLNDGAPVNGHRPSVDVL"
     gene            complement(809..>1011)
                     /locus_tag="VII37_09280"
     CDS             complement(809..>1011)
                     /locus_tag="VII37_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5069972.1"
                     /translation="EKRFGLLVDSLVGEEELVIKALPSDIVASDLVSGASILGDGTVV
                     LILNVPAVVSRFSKALPMEAIA"
BASE COUNT          243 a          295 c          265 g          208 t
ORIGIN      
        1 aacagcacat ccacggaagg tcgatggcca ttcacaggcg ctccatcgtt cagaactacc
       61 atgtcgcccc ggggcatgcg acgaaccatc atgtggcgat ttccgggaca caaaagaacg
      121 cgacccgcta aaagaagatc gccggagcgg gcttcgtgaa tgtccaaggc cgagcattcg
      181 tccaaacgcc gcgcaaacat ttccgtgaat ccctcgggca tgtgctgcac gatcaaaatg
      241 gaggacggaa aatccgcagg aagttgcgaa agcatgaact gcaacgcgtt tggtccgccg
      301 gtggaaatgc caatggcaat aattcgattg ggcggcaccg cgattccaga tcttttcttt
      361 aaaccgacgg gcgtatcgtt gatcacggcc ggcggcaagc gatgtgttga agcacgcttg
      421 gcaaccttga ttttttcgac aagctgatcg gcgatagcgt ccaggtggcc gaccgcggcg
      481 tcctgcggct tggcgacgaa atcaacggca cccagagcga gcgccttaaa tgttgaatag
      541 gcgccttctt tcgagtgcgt gctgaacaaa attaccggca ccggggcgct tttcatgatc
      601 agccgcagcg tttccatacc atccatacgc ggcatttcca gatccagggt gaccacgtcc
      661 ggacgcaact cttcgatttt cttaagcgcg aaagctccgt ccatggctgt gccaaccact
      721 tcgatagccg catcgcgcgt caaaattgcc gggataagct tgcgcatcaa ggcggagtcg
      781 tcgacgacta gaactttgat tcgctcgctc atgctattgc ttccatgggt agggcctttg
      841 agaatcgaga taccaccgca ggtacgttca aaatcagcac aactgtccca tcgcccaata
      901 ttgaagcgcc gctcaccaaa tcgctggcca cgatgtcgct cggcagcgct ttgatgacca
      961 gctcttcctc gcccactaaa gaatccacaa gcaggccaaa acgcttttcc g
//
LOCUS       DATCBB010000841         1372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_482614, whole genome shotgun sequence.
ACCESSION   DATCBB010000841 DATCBB010000000
VERSION     DATCBB010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482614"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VII37_09285"
     CDS             <1..224
                     /locus_tag="VII37_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069973.1"
                     /translation="LSAISAQARMRELLLPLLLLPLLTPVLVASTQVTVGLLDVSPFL
                     EWKGVGFLVIFDVVFLTALWLFGEYLLEE"
     gene            230..907
                     /gene="ccsA"
                     /locus_tag="VII37_09290"
     CDS             230..907
                     /gene="ccsA"
                     /locus_tag="VII37_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5069974.1"
                     /translation="MKKQVLAAAVVFFLVGAAAYGAFFVAPTERTMGPIQRIFYLHVS
                     SAWTGLTAFFICFVANLLYVFRRQSQWDWLSVSAAEVGLVFTSVVLVTGPIWAHPVWG
                     IWWTWDARLTSTFILWLLYACFLLLRTLVEEPDRRRLFSALFGIFAFIDVPLVFGSIR
                     WWRTQHPQPVVMGGSGSGLDPTMWKVLFFSWVAMLALMALLIRARYLMESARGALETL
                     RQESEAV"
     gene            904..1047
                     /locus_tag="VII37_09295"
     CDS             904..1047
                     /locus_tag="VII37_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069975.1"
                     /translation="MKNLNSIFAAYLIAWGIFFVYYLTVARRAAALHEDIERLKNSLH
                     RGK"
     gene            1049..>1372
                     /locus_tag="VII37_09300"
     CDS             1049..>1372
                     /locus_tag="VII37_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY5069976.1"
                     /translation="MAGKKRVGIVLFQLGGPDSLDAVEPFLLNLFLDPDIIPLGPLGL
                     LRTPIAKLISSRRSKPVIGRYAQIGRRSPIATLTERQRVRLLRALSPYIDPVLVVAMR
                     YWHPTT"
BASE COUNT          206 a          431 c          381 g          354 t
ORIGIN      
        1 cgctttccgc catttccgcg caggctcgca tgcgcgaatt gctgctcccg cttttattgc
       61 tcccgttgct gaccccggtg ctggtggcca gcacccaagt aaccgtaggg ctcttggacg
      121 tgtcgccgtt tctggaatgg aaaggcgttg gcttcctggt aattttcgac gtggtctttt
      181 tgaccgcgct atggcttttc ggcgaatatc ttctggagga atagtgggca tgaagaaaca
      241 ggtcttggcc gccgccgtgg ttttcttttt agtaggtgcg gccgcctatg gcgcattctt
      301 tgtcgcgccc accgagcgca ccatgggccc catccagcgc attttctatc tgcacgtctc
      361 cagcgcctgg accggcctca ccgccttctt catctgcttc gtcgccaatc tgctatacgt
      421 ctttcgccgc cagtcgcaat gggattggct cagtgtctcg gcggcggaag tgggtttggt
      481 cttcaccagc gtagtactgg tcactggccc catctgggcg catcccgtct ggggcatctg
      541 gtggacctgg gacgcgcgcc tcacctccac atttattctc tggctgctat acgcctgttt
      601 tctgctgctg cgcactttgg tggaagaacc ggatcgccgc agactcttca gcgcactctt
      661 cggaattttc gccttcatcg acgtgccgct cgttttcggc tccattcgct ggtggcgcac
      721 ccagcacccg caaccggtag tgatgggggg ctccggctcc ggtctcgacc ccaccatgtg
      781 gaaagtgctg tttttttcct gggtcgcgat gttggcgctg atggctttat taattcgcgc
      841 ccgttacctg atggaatcgg cgcggggcgc gttggaaact cttcggcagg aatcggaggc
      901 ggtatgaaaa atctgaacag catttttgcg gcctacctga tcgcgtgggg aattttcttt
      961 gtctattacc tcaccgtcgc ccgccgcgcc gccgcgctgc acgaggatat cgaacgcctc
     1021 aaaaattctc tccatcgggg caaatagcgt ggcagggaag aagcgcgtcg gcatcgtgtt
     1081 gttccaactc ggcggccccg attcgctcga cgccgtcgag cctttcttgc tcaatctctt
     1141 tctcgatccg gacattatcc cgttgggtcc gcttggcctg ttgcgcaccc caattgcgaa
     1201 gctgatttcc tcgcgccgtt cgaaacccgt catcggacgt tacgcgcaaa ttggccgtcg
     1261 ctcgccgatc gcaacgctca ccgagcgcca gcgcgttcgc ctgctgcgcg cgctttcgcc
     1321 ctacatcgat cccgtcttgg tggtggccat gcgttactgg catcccacca cc
//
LOCUS       DATCBB010000842         1319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_124201, whole genome shotgun sequence.
ACCESSION   DATCBB010000842 DATCBB010000000
VERSION     DATCBB010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124201"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VII37_09305"
     CDS             complement(<1..1002)
                     /locus_tag="VII37_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5069977.1"
                     /translation="MGTIYVDGKPFEADAKQNLLHACLSLGFDLPYFCWHAALGSVGA
                     CRQCAVKEFKDEKDTKGKLVMACMTPAKEETRISIHDPEAIAFRAAIVEGMMLNHPHD
                     CPVCDVGGECHLQDMTVMTGHDYRRTRFEKRTFRNQYLGPFLHHEMNRCIQCYRCVRF
                     YREYAGGNDLNAFGIKNTVYFGRSEDGVLENEFAGNLVEICPTGVFTDKTLRRHYTRK
                     WDLRMAPAICGHCGAGCNTTIGERYGLLRRVLTRFNSEVNGYFLCDRGRYGYEFVNSE
                     YRIRAARLDGKPIPADEAAQKLESFVSVGKAIGIGSPRASLEANFALRTLVGKDRFFA
                     "
     gene            complement(1012..>1319)
                     /locus_tag="VII37_09310"
     CDS             complement(1012..>1319)
                     /locus_tag="VII37_09310"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5069978.1"
                     /translation="TCPVGMVSNLIHFFAQESCGWCTPCWSGLNWARQILKALEDGEG
                     KPGDLEKLEFQTKFWAPGHTFCALAPGAAEPLQSALKYFRDDFERHIQDKGYAFQRR"
BASE COUNT          279 a          398 c          357 g          285 t
ORIGIN      
        1 tgcaaaaaaa cgatccttcc cgaccagcgt gcgcagcgcg aagttggctt cgagcgacgc
       61 tcgcggcgag ccgataccga tcgccttacc gacgctaacg aaagactcaa gcttttgcgc
      121 tgcttcatcg gcaggaatgg gtttcccatc gagccgcgcc gctcgaatcc tgtattcgct
      181 attcacgaac tcatagccgt agcgcccgcg gtcgcagaga aagtagccgt tcacctcgct
      241 gttgaacctg gtcaaaactc ggcgcagcaa cccatagcgc tcgccgatgg tggtattgca
      301 gccagctccg caatgaccgc aaatcgcggg agccattcgc agatcccatt tccgcgtgta
      361 gtggcgcctc aatgttttat cggtgaaaac acccgtggga cagatttcca ccaggttacc
      421 cgcaaactcg ttttcgagaa ctccgtcttc gctgcggccg aagtaaacgg tgttcttaat
      481 gccaaacgcg ttcaggtcgt ttcctccggc gtattcgcga taaaaccgca cgcagcggta
      541 gcactgtatg cagcggttca tctcgtgatg caggaaaggg ccgaggtatt ggttccgaaa
      601 cgtgcgcttc tcaaaccgcg tccggcggta gtcatggccg gtcatgaccg tcatgtcctg
      661 aagatggcac tcgccgccta cgtcgcacac cggacaatcg tgaggatggt tcagcatcat
      721 cccctcgacg atcgcggcgc ggaaagcgat ggcctccgga tcgtgaatgg agatccgcgt
      781 ctcttccttg gcgggcgtca tgcacgccat cacgagtttg cccttggtgt ccttttcatc
      841 cttgaattct ttgacggcac actggcggca cgcgcccacg gacccgagag cagcgtgcca
      901 gcagaagtac ggcaagtcga agcccagcga gagacatgcg tgcagcaggt tttgcttcgc
      961 gtcagcttcg aagggtttgc cgtcgacgta aatagttccc atccggttcg cctatcgcct
     1021 ttggaaagca tagcccttgt cctggatgtg ccgctcgaaa tcatcgcgga aatatttcag
     1081 tgcgctctgc aaaggttccg ccgcgcctgg cgcgagcgcg caaaacgtgt ggccgggcgc
     1141 ccaaaacttg gtttgaaact ctaatttctc cagatctcca ggttttcctt cgccgtcctc
     1201 caatgctttc agaatctgcc tcgcccagtt caagccgctc cagcatggag tacaccagcc
     1261 gcaggattcc tgagcaaaaa agtgaatgag gttcgagacc ataccgacag gacaggtct
//
LOCUS       DATCBB010000843         1031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_124227, whole genome shotgun sequence.
ACCESSION   DATCBB010000843 DATCBB010000000
VERSION     DATCBB010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124227"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1031
                     /locus_tag="VII37_09315"
     CDS             <1..>1031
                     /locus_tag="VII37_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5069979.1"
                     /translation="LIAGLFSLVIFSGMLWGQCSANGPDAQPESSQIQQLFEQKNWAE
                     VVRLAGSIASRSADLNFEYGLALAHLQQWPEARAALIAGEHECPEEKRFAIELAGVAF
                     ELKRNPEATAWLRRGLKIDPKDEYANNFAGTVYLLMGNTNAALKYWNRAQKPYVAALN
                     FDPQLRVQRLILDRAFAFSPAATLKQRDFETTEARLDGLGIFPKYNIVLNARADGNPS
                     GSFDAEFHAQELNGFGSSRVQALISTFGGAAYGTIYPSYFNIRGSAANVESLLRWDSQ
                     KRRAWISVSAPLHDLPQWRWQLEADARNENWAIRRSFTGAAPVLGSLNLQRQTVTGSV
                     MGFPSGRLQ"
BASE COUNT          202 a          271 c          332 g          226 t
ORIGIN      
        1 cactgattgc aggattgttt tctcttgtga ttttttccgg catgttgtgg gggcaatgct
       61 ctgcgaacgg gccggacgcc caaccggaat cttcccagat tcaacagctc tttgaacaga
      121 agaattgggc cgaggtggtg cggctggccg gttcgattgc ttcgcgctcg gccgatctga
      181 atttcgagta cggacttgcc ttggcgcatt tgcaacagtg gccggaggcg cgcgcggcgc
      241 tcatcgcggg cgagcacgaa tgtcctgagg agaagcgctt cgcgatcgag ttggccggcg
      301 ttgcttttga actcaaacgc aatccggagg cgacggcgtg gctgcgtcga gggttgaaga
      361 tcgacccgaa ggacgaatac gccaacaact ttgctgggac cgtttacctg ctgatgggaa
      421 acacgaacgc ggcgctcaag tattggaacc gcgctcagaa accatatgtc gctgctctta
      481 atttcgatcc gcaactgcgc gtgcagcggc tgattctgga tcgtgcattt gcgttttcgc
      541 cggcggcgac gctgaagcag cgcgactttg aaacgaccga ggcacggctt gatgggctgg
      601 gcatctttcc gaaatacaac atcgttttga atgcgcgcgc ggatggaaat cccagtggaa
      661 gtttcgacgc cgagtttcat gcgcaggaac tgaacggctt cggcagcagc cgcgtgcagg
      721 cgctgatttc gacgttcggt ggggcggcgt acggaaccat ttatcccagc tacttcaata
      781 tccgggggtc ggccgcgaat gttgaatctc tgctgcgctg ggactcgcaa aaaaggcgtg
      841 catggatttc cgtttctgcg ccgctgcacg atctgccaca atggcgatgg caactggaag
      901 cagatgcgcg caatgagaac tgggcgatac ggcgctcgtt taccggcgcg gccccggtgc
      961 tgggatcgct gaacttgcag cggcagacgg tcactggatc ggtgatgggc tttccgagtg
     1021 gacgactgca g
//
LOCUS       DATCBB010000844         1125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_222461, whole genome shotgun sequence.
ACCESSION   DATCBB010000844 DATCBB010000000
VERSION     DATCBB010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222461"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VII37_09320"
     CDS             complement(<1..1046)
                     /locus_tag="VII37_09320"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEY5069980.1"
                     /translation="MAHRFTIGVEEEFQIIDPATCELRSHVMQLVSAASPGIVEQVKH
                     EMHQSIVETGTNICDSVHELRIEMHRTRGELVAAAERAGLQVAAAGTHPFSSWIDQVI
                     SPGERYQNIIEELQQLARSLLIFGMHVHVAMPDKQTTIDMMNMVRYFLPHLLALSTSS
                     PFWMGRNTGLKSFRTTVFRRFPRTGIPEQFESWSAYENFVNLLVKLNCIDTGKKIWWD
                     VRPHPTFGTLEFRMCDVTTKVEEAVAIAALTQAIVVKLHRLYKGNMGFRLYRRALIEE
                     NKWRAARYGIEGKMVDFGKEIEVPMRELIPELLEFVDDVLDDLGSRSAVAYVNTIMSE
                     GTSAERQLRVFQETG"
BASE COUNT          220 a          319 c          332 g          254 t
ORIGIN      
        1 ccggtttcct ggaagacgcg cagttggcgc tccgcgctgg tgccctcgct catgatggtg
       61 ttcacgtacg cgacggcgct gcggctgccc agatcgtcca atacatcgtc cacgaattcc
      121 agcagttcgg ggatcaactc cctcatggga acttcgattt ctttgccgaa atccaccatc
      181 ttgccttcga ttccgtaacg cgcggcgcgc cacttatttt cttcgatcag cgcgcggcga
      241 tacaaccgga agcccatgtt gcctttgtag aggcgatgaa gtttcacgac gatggcctga
      301 gttaacgcgg cgatggctac agcctcttcc actttggtgg tcacgtcgca catgcgaaat
      361 tccagcgtgc cgaaggtcgg gtgcgggcgc acgtcccacc agattttttt tccggtatcg
      421 atgcagttga gcttgaccag cagatttaca aaattttcgt aggcgctcca ggattcgaac
      481 tgctccggga tgccggtgcg cggaaacctg cgaaacacgg tggtgcggaa agatttcagc
      541 ccggtattgc gtcccatcca gaagggcgaa ctggtcgaga gcgccaagag gtgcggcagg
      601 aaataacgca ccatgttcat catgtcgatg gtggtttgct tgtcgggcat ggccacgtgg
      661 acgtgcatgc cgaaaatcag caaagaacgg gccagctgtt gcagctcttc aataatattt
      721 tgatagcgct cgccggggga aatcacctgg tcgatccagc tggaaaacgg atgagtgccg
      781 gccgcggcta cctgcagccc cgcgcgttcc gcggctgcta ccagctcgcc gcgggtgcgg
      841 tgcatttcaa tgcgcagctc gtgcacgctg tcgcagatat tggtgccggt ctccacgatg
      901 gactggtgca tttcatgctt cacctgctcg acgatgccgg gcgaagccgc cgacaccagc
      961 tgcatgacgt gcgaacgcag ttcacaggtg gccgggtcga tgatctggaa ttcttcttct
     1021 acgccgatcg tgaagcggtg agccatggtg tggccttccg gttggccagg cgattcgcgg
     1081 gactggactt gagatgcgta tcatacgctt ctcatccaca aaatt
//
LOCUS       DATCBB010000845         1267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_318470, whole genome shotgun sequence.
ACCESSION   DATCBB010000845 DATCBB010000000
VERSION     DATCBB010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318470"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VII37_09325"
     CDS             complement(<1..1161)
                     /locus_tag="VII37_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5069981.1"
                     /translation="MAIFLTGSTGYIGAHVASILLERHTDRLNLLVRAKTEQEANERL
                     WHALQLHLDFPQFHEALETRISVFRGDLTEPRFGLDAADYTHLIGTTESVIHCAASLN
                     RKSEKSCLNVNLRGTLEVAQLARRAQDAHGLRRFSQVSTVAVAGHRSHEVVAEDASID
                     WNRSDYDPYARTKKFCEHMVRELLPDVSRTVFRPSIVLGDSRRAETTQFDMVRAFVFL
                     AGLPLLPFRSTDRIDIVPVDYVAESVVTLHQKPDPAHEIYHLSSGTRSETFGQLTHAL
                     AESAGKMQPMFWPGLERNFSAVVNWLSNSRGTIGRSASLLKVFMPYLVWDTVFENSRV
                     VAEMGRAPESFSKYCYPLLRFSRDHRFSYPYQPWPGSAGLVRENAAAAGVGSA"
BASE COUNT          274 a          361 c          399 g          233 t
ORIGIN      
        1 cgcggaaccc acgccggccg ctgcggcgtt ctcgcggacc aaaccggcgg acccaggcca
       61 aggctgatag ggataggaaa aacgatgatc gcggctgaat ctcagcagag ggtaacaata
      121 cttggaaaag gattccggcg cgcggcccat ttccgcgacc acccgcgaat tctcgaacac
      181 cgtgtcccag acgaggtagg gcatgaacac tttcagcagc gatgcgctgc ggccaatcgt
      241 gccgcgagaa ttcgacagcc agtttacaac cgcggagaaa ttccgctcga gccccggcca
      301 gaacatgggt tgcatttttc cggcggactc ggcaagagca tgcgtgagct ggccaaaagt
      361 ttcggaacgc gtgcctgaag agaggtggta gatctcatgg gctggatcgg gcttttgatg
      421 cagcgtgacg acggactcgg ccacgtagtc caccgggacg atatcgattc ggtcggtcga
      481 gcgaaagggc aatagcggca agcccgcgag aaacacgaat gcccggacca tatcgaattg
      541 cgttgtttca gcgcggcggc tgtcgccgag cacgatgctc gggcgaaaca ctgttcgaga
      601 aacatcaggg agcagttccc gcaccatgtg ctcgcagaat tttttcgtgc gcgcataagg
      661 atcgtagtcg gagcgattcc aatcaatgga agcatcctcg gccactacct cgtggctgcg
      721 gtggcccgcc acggcaacgg tggaaacctg gctaaagcgg cgcagtccgt gggcgtcttg
      781 cgcgcggcgc gcaagttgcg cgacttcgag agtgccgcgc aaattcacgt tcaggcagct
      841 tttttcggat ttccggttga gcgaggccgc gcaatgaatc accgactcgg tcgtgccaat
      901 cagatgcgtg taatcggccg cgtccagccc aaaacgcggc tccgtgagat ctccgcggaa
      961 aacggaaata cgcgtttcga gtgcttcgtg gaactgagga aagtccagat gcaattgcag
     1021 cgcgtgccag agacgctcgt tcgcctcttg ttccgtcttc gcccgcacca gcagatttaa
     1081 gcggtccgtg tgacgctcca gcaaaatgga ggcgacgtgg gcgccaatgt agccggttga
     1141 accggtcaga aagatggcca tggattagtt tttggtcccc gcgcctgacg cagcgcccgc
     1201 atggggcaga tcttccgggc gttccggtgc accattgccg gcgtgctgca aggaaaaagc
     1261 gcggtgc
//
LOCUS       DATCBB010000846         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_59582, whole genome shotgun sequence.
ACCESSION   DATCBB010000846 DATCBB010000000
VERSION     DATCBB010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59582"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VII37_09330"
     CDS             <1..1014
                     /locus_tag="VII37_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5069982.1"
                     /translation="VRNAVQQRRLEEENQTLRNQLGHRYQVIGDSIPMKALRQQIAVT
                     APTNGRVLIYGESGTGKELVAHALHAASLRTKGPFVEVNCAAIPDELIESELFGHLKG
                     SFTGATDNKIGKFEKADGGTLFLDEIGDMSLRTQSKVLRVLEEQRFEPVGSNQTITVD
                     VRVIAATNKNLEQEIAHAHFRQDLFYRLNVIPFFVPPLRDRLQDIPTLARYFLNEFSG
                     AYGKKGRELSDPAMDILVRYPWPGNVRELRNLVERLVIVCPHPRIEPHHLPPELFRGV
                     AESPQQPYSTLYEARSAYEREFILRKLQENRWNMTQTAGALGLERSHLYRKMKSLGIA
                     APD"
     gene            complement(1083..1481)
                     /locus_tag="VII37_09335"
     CDS             complement(1083..1481)
                     /locus_tag="VII37_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069983.1"
                     /translation="MDTSDWAKNLAERKKKELQEKRVKDDKLDAQLDTKWTEVCRAAQ
                     KATDELNKEMGEQYVRFSQDANADKFSLKVGEADNTVRLDRPKRAIQGSRGKTYRLAM
                     VGDTAVWKYDPVDYTSEQIARDEVQQAFEG"
     gene            complement(1535..>2031)
                     /locus_tag="VII37_09340"
     CDS             complement(1535..>2031)
                     /locus_tag="VII37_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069984.1"
                     /translation="APSIAGLSILEDGLVVCAGRATDQCSNPDKPEKKDAPVKLVFYP
                     LDGEVYRLALVSQDQKTKIFFAVIPAPIHATERSCTLEVVSLRLKNQLVLIRGKGFEP
                     NESVEFESKSYEQTHSRTDQVDANGEIQLPVMPFVAGKTGGTMEIKLSGTKCAPALAF
                     KWGN"
BASE COUNT          456 a          584 c          517 g          474 t
ORIGIN      
        1 gtgcgcaacg cagtgcagca gcgccgcctg gaagaagaaa atcaaactct tcgcaatcag
       61 ctgggccacc gctatcaggt catcggcgac agcatcccca tgaaagcgtt gcgccaacag
      121 atcgcggtga ccgcgcccac aaatgggcgg gtcttaattt acggcgaaag tggaaccggc
      181 aaggaattgg tggcgcacgc cctacacgcc gcgagcctca gaaccaaggg ccctttcgtg
      241 gaggtaaatt gcgcggctat cccggacgaa ctaattgagt cagaactttt cggccacctg
      301 aagggcagct ttacaggagc taccgacaat aaaatcggca aatttgagaa agccgatggc
      361 ggcacgctat ttctggacga aatcggcgat atgagtttgc gcacacaatc gaaagtgttg
      421 cgcgtgctgg aagagcagcg ctttgagccg gtgggctcga atcaaacgat tacagtagac
      481 gtgcgcgtca tcgccgccac caacaaaaat ctcgaacagg aaatcgcaca cgcacatttc
      541 cgccaggacc ttttctaccg cctgaacgta attccctttt tcgttccgcc actgcgcgac
      601 cgcttgcagg acatccccac actggcgcga tattttttga acgagtttag cggcgcctat
      661 ggaaaaaaag gccgcgaact cagcgacccc gccatggata ttttggtgcg ctacccgtgg
      721 ccagggaatg tgcgagagct ccgcaatcta gttgagcgct tggtcattgt gtgcccgcac
      781 ccacgcatcg agcctcatca cttgccacca gaattatttc gcggcgtggc cgaaagtccg
      841 cagcagccct attccacact gtacgaagcg cgcagcgcct acgagcgcga attcatcctg
      901 cgcaaattac aggaaaatcg ctggaacatg acacaaaccg ctggcgcgct gggcctcgaa
      961 cgcagccacc tataccgcaa aatgaaaagc ctgggcatcg ccgccccgga ttagctaggc
     1021 caaacacatt cgtcgatgtc tcggtttttt aaggaggtcg atacgcctcg cgcgcagcag
     1081 ccttaacctt cgaaggcttg ctgtacttcg tcgcgcgcga tttgttccga agtgtaatcg
     1141 actggatcat atttccagac cgccgtgtcc cctaccatag cgagtcgata ggtttttcct
     1201 cggctgccct gaattgcccg cttgggccga tcaagccgaa cggtgttatc cgcctctcca
     1261 actttcagag agaatttgtc ggcgttagca tcttgagaga aacgaacata ttgctcgccc
     1321 atttctttat ttagttcgtc agtggccttc tgcgccgccc ggcatacttc cgtccacttc
     1381 gtatccaatt gcgcatcaag cttgtcgtct ttaacacgct tctcttgcag ctctttcttt
     1441 tttcgttcgg ccaaattctt ggcccaatct gaagtgtcca tgaaatctcc cgccctagta
     1501 tggcgttaca aatctatcta agccaaatcg gctctcaatt tccccattta aaggcgagcg
     1561 ccggtgcgca ttttgtcccg ctgagcttga tttccatcgt gccgccagtt tttcctgcaa
     1621 cgaatggcat cacaggcagc tgaatctccc cattggcgtc cacttgatct gtgcgcgagt
     1681 gagtttgctc gtacgattta gactcaaatt cgaccgattc attcggctca aatcctttac
     1741 cgcggatcag gactagctga tttttcagac ggaggctgac cacttcgagc gtgcaactcc
     1801 gctctgtggc gtggatcggg gccgggatga ccgcaaaaaa gatttttgtt ttttgatctt
     1861 gggatactaa agcgaggcga tagacttcgc catccagcgg gtaaaacacc aatttcaccg
     1921 gcgcgtcttt cttttctggc ttgtccgggt tgctgcattg gtcggtggcc cggccagcgc
     1981 aaacgacgag cccgtcttcc aatatgctga ggccggcgat ggaaggagcg g
//
LOCUS       DATCBB010000847         1472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_286052, whole genome shotgun sequence.
ACCESSION   DATCBB010000847 DATCBB010000000
VERSION     DATCBB010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286052"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VII37_09345"
     CDS             complement(<1..1372)
                     /locus_tag="VII37_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069985.1"
                     /translation="MNPGDLRVAPETCGSSGCHASETRAVSTSMMTHSGMLWGAALYN
                     NGGYPTKNTRFGESYDRNGKPESIKTSPTPTPEETRAKGVLLELDPLYRWETSQPGNV
                     LRVFERGGRKKGEVGNPIADEDPGKPEDKLSDRGFGTELRTDPVFLGLQKTRLLDPVM
                     SLPGTNDHPGDYRASGCSACHVIFANDRDAAHSGGYAQFGHSGLSASADATISKTERG
                     HPIRHVFTRSIPSSQCMICHIHPGTNMVTTYFGLTWWDNEIDGDKMYPPQQRNPSEEQ
                     RYQALLRNPEGAAARGLWNDDKFLEQVGSAEFNSKLKTTQFADFHGHGWVFRAVFNHD
                     RKGNWLDKDGNIISFEDPERFSKAVHLADIHLEKGMQCNDCHFAQDNHGNGKIYGEPR
                     AAVEIDCIDCHGTIRQKTTLVTSGPSAPETFSAVNGVRVPDRGHHLDTLRTPWGQRRF
                     EWRGAAL"
BASE COUNT          306 a          426 c          385 g          355 t
ORIGIN      
        1 tgagtgccgc gccgcgccat tcgaaacggc gttgtcccca aggcgtgcgc agggtatcga
       61 gatggtggcc gcggtctgga acccgcacac cattcacggc actaaatgtt tccggtgctg
      121 agggacccga ggtgaccagc gtagtttttt gccgaatggt gccgtgacaa tcgatgcagt
      181 cgatttccac cgcagcgcgc ggctctccat aaattttccc attaccgtgg ttgtcctgcg
      241 caaaatggca atcgttgcat tgcatccctt tttccagatg aatgtcagcc aggtgcacgg
      301 ctttagaaaa tcgctcagga tcttcaaaag aaataatgtt gccatccttg tccagccaat
      361 tgcctttgcg gtcgtgatta aacaccgcgc gaaataccca gccatgtccg tgaaagtccg
      421 caaactgcgt ggtcttcaat ttggaattaa attccgcgct gccgacttgc tccaaaaatt
      481 tatcgtcatt ccacaggccc cgcgccgccg caccttccgg attgcgtagg agcgcctgat
      541 aacgttgttc ttcgctggga ttgcgctgtt gcggcggata cattttgtcg ccatcgattt
      601 cgttgtccca ccacgtcaag ccgaaatagg ttgtgaccat gttcgtgccc ggatgaatat
      661 ggcaaatcat gcattggctc gaaggaatcg accgcgtgaa aacatgcctg atgggatgac
      721 cgcgttcagt tttggaaata gtcgcgtccg cggacgcact cagtccggaa tgtccaaatt
      781 gtgcataacc accggagtgc gccgcgtcgc gatcattggc gaaaatcacg tggcaagccg
      841 agcacccgct ggcgcggtaa tcgccaggat gatcgttagt cccaggaagt gacatgaccg
      901 gatcgagaag tcgcgttttt tgcaacccca gaaaaaccgg atcggtccgc agctctgtcc
      961 caaatccgcg atcgctcaat ttgtcttcag gtttgccggg atcttcgtcc gcgatcggat
     1021 tgcccacctc gcctttcttg cggccccctc gttcgaacac ccgcaacaca ttgcccggct
     1081 gcgaagtttc ccagcgataa agaggatcaa gctccagaag cactcctttg gcgcgcgttt
     1141 cttccggcgt tggagtaggc gacgttttta tcgattcggg cttgccgttg cggtcgtaac
     1201 tttcgccgaa gcgcgtattt ttagtcgggt acccgccgtt gttatagagc gccgcgcccc
     1261 acagcatccc cgagtgcgtc atcatgctgg tggagaccgc acgcgtttca ctcgcatggc
     1321 agccgctcga tccgcaggtt tccggcgcca cgcgcaaatc gccaggattc acaaatttta
     1381 tatattcgta cgattccttt agccacgcgg tgaaaacgcg ttcaggtaca gccgtgccct
     1441 ttctaaacac tgcatttttc ggatgcacat gc
//
LOCUS       DATCBB010000848         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_222630, whole genome shotgun sequence.
ACCESSION   DATCBB010000848 DATCBB010000000
VERSION     DATCBB010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222630"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VII37_09350"
     CDS             <1..242
                     /locus_tag="VII37_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069986.1"
                     /translation="QKRISIPSDVYTGIWWYARFPNHYSGEGALATKELGEFEMNAWI
                     NAIAAAIRAAQADDQSLRLQNEFYEKSKHPLDTRP"
     gene            261..2192
                     /locus_tag="VII37_09355"
     CDS             261..2192
                     /locus_tag="VII37_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY5069987.1"
                     /translation="MRSRNELNAADRALGLHTSITRRDFLGSTLLASGGALLGGLTPA
                     QLLAESDDWAGYGAVGEYRHSSGNPREVMLAGHKMRDNAYPNPDMDALDTGELYDCVI
                     VGAGISGLAAALFFQRQTASHAKCLLLDNHPIFGGEAKQNEFQVDGQRLTAHQGSAIY
                     LVPYPYSFVANFYHSIGLHAPKLSYQTWSGSDPEMTLGRTPYDSAGLSIGQYGFWFGA
                     KFGQKPGVWLIDPVGKQMQGAPLAAKTRSELYGWFSGQAATKSKFVRPKFEGDDISRR
                     LDSITLEQHYMEQFGLSRETIRTFLSPVEGGGSGLGPDALSAYNDYAAEMLHPYEAGG
                     DAVQMFPGGNTTIARLMVKTMLPSAVDGPATVEGVTQGTVNFSALDVSESPIRLRLSS
                     TVVSVRHDGDPQKSDTVSVVYAKQGKLFRVRARSAVMAGGSWTTKHMVRDLPEEHRTA
                     YAQFYRSPSMMVNAAVRNWRFLYKMGITGCRWFDGVGNYMDVRKMAKCGTGSATIGPD
                     SPVVLSVKVLYSYPGESTEAQGIRGRGELLNTSFREYEERLRQQFTDMFSGSGFDARR
                     DIAGIILNRWGHAYLSPQPGFFFGKDGKQAPRETLRSAPFGRIAFANTDLAGAMDHRY
                     SILEAQRAVGQLLDQVFTG"
     gene            complement(2221..2577)
                     /locus_tag="VII37_09360"
     CDS             complement(2221..2577)
                     /locus_tag="VII37_09360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5069988.1"
                     /translation="MQEPTSTGPWRSIAVRDRRYSIRYPFAADAELLDLETGVTISGV
                     TSDLSIAGAFVCASKAFKVGCRIRATLTRKDQVVEALAVIRVFKPRVGMGVEFLDVES
                     PFDQTLFRWIEQLRRK"
     gene            2860..>3521
                     /locus_tag="VII37_09365"
     CDS             2860..>3521
                     /locus_tag="VII37_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069989.1"
                     /translation="MRADGLRQSDRINFRMPVEASWFGDGATFKRAATTMLVSKNGGV
                     LRLAERLTAGQELTLRRQQDGDVWKSVRARVVAEIDQDSEGFLYAFQILEPRSDFWDI
                     EFPVLNKADEAIARLLMECSFCERREVVHLNALELKSFEMRKCVARVCSQCVAPSIWI
                     EAQSEMPAPTVEGYVTNIDNHVIPRRKRPRVKARVLACIRRRGFEEEIAVCEDLSKGG
                     LS"
BASE COUNT          808 a          903 c          994 g          816 t
ORIGIN      
        1 accagaaacg gattagtatt ccgagtgacg tttacaccgg catttggtgg tacgcgcgtt
       61 tccccaatca ttattctggc gaaggtgcac tagccaccaa ggaacttggt gaatttgaaa
      121 tgaacgcgtg gattaacgcc attgccgcgg ctatccgcgc tgcacaagcc gacgatcaaa
      181 gcctacggct gcaaaacgaa ttttacgaaa aaagtaagca tccgctcgat acacggcctt
      241 agtaagttag ttgcaaattc atgcgctcga gaaatgaatt aaacgccgct gatcgcgccc
      301 tgggactaca cacttcaatt accaggcgtg attttctcgg ttcgacgctg ctcgcttccg
      361 gcggcgcgct gcttggcggc cttactcccg cgcaattgct ggcggaatca gatgattggg
      421 cgggctatgg tgccgtcgga gaatatcgcc attccagtgg caacccccgc gaagtcatgc
      481 tcgcgggcca taagatgcgt gataacgcgt atccaaatcc ggacatggat gcgctggata
      541 cgggcgaatt gtatgactgc gtgatcgtcg gggccggaat tagtgggttg gccgcggcgt
      601 tattttttca acgacagaca gcttcgcacg ccaaatgcct gttgctcgac aatcacccta
      661 tttttggcgg cgaggccaaa cagaatgagt ttcaggttga cggtcagcgg ctgacggctc
      721 atcaaggttc ggcgatctac ctggttccgt acccgtacag tttcgtcgcc aatttttacc
      781 actccatcgg cttgcatgcg cccaagcttt cctatcaaac gtggtctggc agcgatcccg
      841 agatgactct gggacgcacc ccctacgact ccgcgggatt aagcatcggc caatatggat
      901 tttggttcgg tgcgaaattt ggacagaagc ccggagtgtg gctgatcgat cccgtaggga
      961 aacaaatgca gggcgcgcca ttggctgcaa aaacaagaag cgaattgtac ggttggttct
     1021 cgggccaggc tgcgactaaa tcaaaatttg tgcggcctaa atttgagggc gatgatattt
     1081 cccggcggct cgattccata acgctcgaac aacattacat ggagcaattc ggacttagcc
     1141 gagaaaccat tcgaactttt ttgtcaccgg tggaaggtgg cggttctggc ctgggacccg
     1201 acgcgctttc ggcttacaac gattatgccg cggaaatgct gcatccctac gaagcgggag
     1261 gggatgcggt gcaaatgttc ccgggcggca acactaccat tgcgcggttg atggtgaaga
     1321 cgatgttgcc cagtgcggtt gacggccctg caactgtcga aggagtgacg caaggaacgg
     1381 tgaacttctc ggcgctggat gttagcgaat ctcctattcg gctacgcttg tcgtcgacag
     1441 tagtttccgt acggcacgat ggagatccgc aaaagtcaga taccgtgtcc gtcgtttatg
     1501 ccaagcaggg caaattattt cgcgtcaggg cgcgctccgc ggtgatggcc ggaggaagct
     1561 ggactaccaa gcacatggtc cgcgatcttc ccgaggagca tcgcaccgcg tatgcgcagt
     1621 tctacaggtc accgtcgatg atggtaaacg cggcggttcg caactggcgt tttctttaca
     1681 aaatgggaat caccggctgt cgttggttcg acggcgttgg caactacatg gatgtacgta
     1741 aaatggccaa gtgcggcaca gggagcgcga caattggacc agattcgccg gtagtgctct
     1801 ccgtaaaagt gttgtactcc taccccggcg agtctaccga agcgcaaggc attcgtggac
     1861 gcggcgaatt gctgaacact tctttccgtg aatacgaaga gcgcttgcgg caacaattca
     1921 cggatatgtt ttctggctct ggattcgatg cgcgaaggga tatcgcggga attattttga
     1981 atcgctgggg ccacgcttac cttagcccgc aaccggggtt tttctttggt aaagacggca
     2041 agcaggcacc gcgtgaaact ctgcgcagtg ctccgttcgg gcgaattgcg tttgctaata
     2101 ccgacctagc tggcgcgatg gaccatcgat attcgattct tgaggcgcag cgggccgtgg
     2161 ggcaacttct tgaccaggtg tttaccggct gatggcgggt gattttacgt ttctgcgtag
     2221 ttacttgcgg cggagctgct caatccaacg aaacaaagtt tgatcgaagg gcgattccac
     2281 gtccagaaat tcgacgccca tgccaacacg aggcttgaaa acccgaataa ccgccagcgc
     2341 ttcgacgact tgatctttgc gcgtcagagt tgcgcgaatc cggcatccaa ctttaaaagc
     2401 tttgcttgca cagacaaatg cgccggcgat ggaaagatcg gaagttactc cactgatcgt
     2461 cacgccagtt tcgaggtcaa gcaattcggc gtctgcggca aatggataac gaatggaata
     2521 tcggcggtcg cgcactgcga tggagcgcca cggaccagtt gatgtcggtt cttgcatgaa
     2581 gattacgtta ctcacaatcg tatgaggccc gcacaacgat acagcccctg aaaactcctc
     2641 gtttacatac ataaattgtc tcttgaaacc gtaatctgta gtggtatttt gatgacttct
     2701 atttgcgaaa aagctaatag ctggccgcct cttcagcata tctgctcgtg aattagcgtt
     2761 attagccgct acgacaaagc accccagacc cagccagttt gagcgcaaaa ccaaatgcta
     2821 ttctgccctg gtacttctct ctctaggcga atactacaaa tgcgcgcgga cgggctgcgg
     2881 caaagtgatc gtataaattt ccggatgccg gtggaggcat cgtggttcgg tgacggtgct
     2941 acgttcaagc gcgccgcaac aactatgctg gtgagcaaaa atggtggcgt attgaggctc
     3001 gcggagagac tgaccgccgg ccaggaactg accctgcgcc gccagcaaga tggcgatgtc
     3061 tggaaatccg tgcgagcccg ggtagtcgct gaaattgacc aggattccga aggctttctt
     3121 tacgcttttc aaatcctgga accccgctcg gatttttggg acattgaatt tccagtcctc
     3181 aataaagccg atgaagccat cgcgcggttg ttgatggagt gcagtttttg tgagcgacgc
     3241 gaagttgtgc acttgaacgc cttggagctt aaatccttcg aaatgcgcaa gtgcgttgca
     3301 cgcgtttgca gccagtgcgt agccccctcg atctggattg aagcgcaatc cgaaatgccc
     3361 gcgcctacag tagagggtta cgtcacaaac atcgataacc acgtgattcc gcggcgcaaa
     3421 cgcccgcgtg ttaaggcccg cgtgctggca tgtattcggc ggcgaggctt tgaggaagaa
     3481 atcgcggtgt gtgaggactt atccaagggt gggcttagtt t
//
LOCUS       DATCBB010000849         1399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_59645, whole genome shotgun sequence.
ACCESSION   DATCBB010000849 DATCBB010000000
VERSION     DATCBB010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59645"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1012)
                     /locus_tag="VII37_09370"
     CDS             complement(125..1012)
                     /locus_tag="VII37_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY5069990.1"
                     /translation="MPNTWTFTHWSEFTLRNTLHVVGIIVVALLLNRLLRVVTNLVIK
                     RAASQTRAAQAREQQTRTLAGLLFSAGTKIVWGIALLTAMPEFGINVTPIATLAGLAS
                     LAIGFGAQNLVRDLITGFYIILEDQYIVGDTIQVADTIGRVEHVTLRRTVVRDARGAL
                     VTIANGDIRTVSNLSRDWSQAFVDISLSPDAPLEQTLRSLESAAAEMRADPAWSKALV
                     DGPRILGVQSFDRNATTVRLQVRTLPNHQDEISRELRRRIHVELQRQGIPVSIVQRFE
                     FAGAVPASEPVKAGSLSNQ"
     gene            1112..>1399
                     /locus_tag="VII37_09375"
     CDS             1112..>1399
                     /locus_tag="VII37_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069991.1"
                     /translation="MIAGGQERRKFLRLFCGLSITVAPMIFLQGKSRAQQDTRPLNNA
                     DGKPADENGSFPPGSRKAALEENEKDIKKKVEKLFQLASELKDEVDKTDSAK"
BASE COUNT          356 a          362 c          405 g          276 t
ORIGIN      
        1 ttgaggatga aaatgttcca cgaaccacga gcgtgcctgg cgcaaacgcg gttcgcgcct
       61 gaggtcataa agacgcagca tcaaatttac ttcgtcataa ctgatctggc tcatatgttg
      121 ctcctcactg atttgaaaga ctgccagcct ttacaggttc cgaagctggg acggctccgg
      181 cgaactcaaa acgctggacg atagatactg gaataccctg acgctgaagc tccacatgaa
      241 tgcggcgccg cagctcacga gaaatttcat cctggtgatt tgggagggtg cgcacctgca
      301 atcgcacggt tgtggcgttg cgatcaaaac tttgaacacc cagaatgcgc ggtccgtcga
      361 ctaatgcctt ggaccacgcc ggatcggcgc gcatctccgc ggccgccgac tccaatgaac
      421 gcaaagtctg ttctaaggga gcgtcgggcg aaagcgaaat gtccacgaaa gcctgggacc
      481 aatcacggct cagattgctt accgtgcgaa tatctccgtt ggcgatggta accaatgcgc
      541 cgcgggcatc gcgcaccacg gttcgccgca aggtgacatg ttcgacgcgg ccgatggtgt
      601 cggcgacctg aatggtgtcc cctacgatgt actgatcttc gaggatgatg taaaaaccgg
      661 tgatgagatc acgaacaaga ttttgcgcgc caaaacctat tgcgagactg gctagcccgg
      721 cgagtgtagc gatgggcgtc acattgatgc cgaactccgg catggcggtg agaagtgcaa
      781 tgccccagac aatcttggtt cccgcgctaa aaagcaatcc agccaacgtg cgggtttgtt
      841 gttcgcgggc ctgggccgcg cgcgtttgcg aggccgctcg cttgatgacc agatttgtga
      901 ccacgcgcag cagccggttc agtagcaacg caacaacgat aatccccacc acgtgcaggg
      961 tgttgcgaag ggtgaattca gaccagtgcg tgaaagtcca agtattgggc ataaacttta
     1021 caggagggcg agtataacat tggaactgca gccaggctgt acgcgggaga ggttcggcca
     1081 tgcaggcaag tcgcggtaaa ggaggcccac gatgattgca ggcggccaag agcgccgtaa
     1141 attcttacgc ctattttgcg ggttgagtat cactgtcgct ccaatgattt tcctgcaggg
     1201 gaaatcacgt gcgcagcaag acaccaggcc cctaaataat gctgacggca agccggcaga
     1261 cgagaacgga tcttttccac ccgggtcgcg taaggctgcg ttggaagaaa atgaaaaaga
     1321 catcaagaaa aaagtggaga aattattcca gctggcaagc gaactgaagg acgaagtgga
     1381 taaaaccgat tccgcaaaa
//
LOCUS       DATCBB010000850         1384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_59660, whole genome shotgun sequence.
ACCESSION   DATCBB010000850 DATCBB010000000
VERSION     DATCBB010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59660"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VII37_09380"
     CDS             <1..224
                     /locus_tag="VII37_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069992.1"
                     /translation="GLPVGRIARAFAKRFGTRQQQPQIISADAIAAELGEWARGFGLD
                     QRLSGEKARRELGWKPTHLDPEAEIARLP"
     gene            complement(479..688)
                     /locus_tag="VII37_09385"
     CDS             complement(479..688)
                     /locus_tag="VII37_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069993.1"
                     /translation="MAARLGRAGTVGVNDISIIALAKTLNLPVVSMESAVNNDASMKR
                     RIPDILEDVQQMYFSDYCRAEGLKF"
     gene            complement(731..1195)
                     /locus_tag="VII37_09390"
     CDS             complement(731..1195)
                     /locus_tag="VII37_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069994.1"
                     /translation="MGRFLGGLLARGAVRAAVSNSHSSTPLKSYTSDVLTVEQLATCI
                     KKAGKLDGESTRLDAERGSLLALTSQVELSSTAIEFQRSRIDQYSKASVDAFNASIGT
                     HNRLVMNAKARNAEFNSGIDKHNGEANAFNSECAKKYYAEDLADAQKLAASN"
BASE COUNT          302 a          381 c          390 g          311 t
ORIGIN      
        1 aagggcttcc cgtcggccgc attgcgcgtg cttttgcaaa gcgcttcggc acgcggcaac
       61 agcagccgca gattatttcc gccgatgcga tcgcggccga acttggcgaa tgggcgcgag
      121 gctttgggct cgaccagcgg ctgagcgggg agaaggcacg ccgcgaactc ggatggaaac
      181 cgacgcatct cgatccggaa gctgaaatcg ctcggctgcc gtgaaggctg ccggatacta
      241 cactcgtcat gcccgggctt gtcccgggca tccacgtccc aaagcggcga cagcaagaaa
      301 gacgtagatg gccgggacta agcccggcca tgacggaaag tgaaatcgcg cggtgtagat
      361 gccgtggaaa gcgagttcgg ttatcttagt cgctagttgt tctggcacta tccttgcgcg
      421 gagcatcgct ccgcgactgg cggcggcggt agaaaacgtc gcaagcgaaa tcgcccgctt
      481 aaaacttcaa tccttcagca cggcaatagt cgctaaaata catctgctgg acgtcttcca
      541 aaatgtcggg gatgcgtcgc ttcatcgaag cgtcgttatt cacggctgat tccatgctga
      601 ctaccggcag gtttagagtc ttggccaagg cgatgatcga aatgtcgttg acgccgacgg
      661 tacctgcgcg acctaagcgg gctgccatca gccatagtcc ccccatttcc ttgcggcttc
      721 aatccgaagt ttagttgcta gcggctagtt tttgtgcgtc cgctaaatct tcggcgtaat
      781 attttttggc gcattcggaa ttgaatgcgt tggcctcacc gttgtgcttg tcaatgcccg
      841 aattgaattc cgcgtttctc gcttttgcgt tcatgacgag tctattgtga gtcccaatag
      901 atgcgttgaa agcatcgacc gaagctttgc tgtattgatc gatgcgactc cgttgaaact
      961 caatagccgt gctggacaat tcgacctgtg acgttagcgc aagcaatgaa ccacgctccg
     1021 cgtcgagtcg cgtactctcg ccgtccagtt tgccagcctt cttgatgcaa gttgcgagct
     1081 gttcgacggt aagaacatca gacgtgtagg attttagcgg tgtgctggaa tgcgaatttg
     1141 acactgcggc acgcaccgcg ccgcgcgcca acagccctcc aagaaaacgg cccatcccac
     1201 ctgcgattgc cggggatgca taacaaaata caaaggcagc gaggatgaaa gctgcggtag
     1261 gcctgcgcac catcggaaat ccgcctccca accaatgaac cgtcacaacc tatggcggta
     1321 ggttagctga ataatcgcct atcagcttag gctgcttcct tgcgggatca ttttggatct
     1381 aggg
//
LOCUS       DATCBB010000851         1794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_91621, whole genome shotgun sequence.
ACCESSION   DATCBB010000851 DATCBB010000000
VERSION     DATCBB010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91621"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VII37_09395"
     CDS             complement(<1..185)
                     /locus_tag="VII37_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5069995.1"
                     /translation="MNDAFDIRNMESHIEVHKFRGGAIVAATLLALMFQAFVPVYFAK
                     AAMIDLPLLITIYFGLSR"
     gene            complement(182..1168)
                     /gene="mreC"
                     /locus_tag="VII37_09400"
     CDS             complement(182..1168)
                     /gene="mreC"
                     /locus_tag="VII37_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5069996.1"
                     /translation="MAGIPSRHKSIFLLAGVVLLQILLLALQIKRDSEGRLIRVWTVG
                     AFSPFERAGNHGIGHLRETWRHYFALQNTSLENERLHRENDDLKMQVNQLQSKASEAD
                     RLALLLNFHKSHENVPMLGARVIATSAGTASATIMLDRGSKDHIKKNMGVITPEGVVG
                     KVVEVYDNTSEVLLLTDKDSGVGAMLGTSRKQSPVGGAGEPLLVMKYVANDDIVNVGE
                     RVVTSGMDKIFPRDLPIGTITEIKPGNPFKSIRIRPSADLERLEEVIVLLTLQPLQLN
                     QESETAKPVATKPAAETPKPPVTGTAAKPAPTAPVTQAPVPDSTNAAAVKPE"
     gene            complement(1331..>1794)
                     /locus_tag="VII37_09405"
     CDS             complement(1331..>1794)
                     /locus_tag="VII37_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5069997.1"
                     /translation="EMDEAVMHYLKRKYNLLVGERTAEQIKMELGSAYPLEKPLTMEV
                     KGRNLIEGVPRTVTIDDSEIREALSECVATILNAVRVALERTPPELSADISDRGIVLT
                     GGGALLKNLDKRIREETGLPVSIADDPLASVVLGTGKMLSDFRLLRQVCID"
BASE COUNT          378 a          483 c          532 g          401 t
ORIGIN      
        1 cggctcagcc caaaataaat ggtgatcagc aaaggcaaat cgatcatcgc ggccttcgca
       61 aaataaacgg gcacgaaggc ctgaaacatc agggcgagca gcgtggcggc gacaatggct
      121 ccgccacgga acttatgcac ctcgatgtgc gattccatgt tgcgaatgtc gaaggcgtcg
      181 ttcattcggg ttttaccgcc gcagcgttgg tggagtctgg aaccggcgct tgggtgacgg
      241 gggcagtcgg agcaggtttg gcggcagttc cggttaccgg cggcttggga gtttccgcgg
      301 cgggcttagt ggccacaggc ttcgcagttt ccgattcctg attcaattgc agcggctgaa
      361 gggtcagaag aacaattact tcttccaagc gctccagatc ggccgagggg cgaatgcgaa
      421 tgctcttaaa gggattgccg ggtttgattt cggtgatagt tccgatggga agatcgcgcg
      481 gaaaaatttt gtccataccg ctggtcacta cccgttcgcc gacgttcact atgtcgtcgt
      541 tagcgacgta cttcatgacc agcaacggct cgccagcacc gccaacggga ctttgcttgc
      601 gggaggtgcc gagcatggcg ccgaccccgc tgtctttatc ggtcagcagc aggacttccg
      661 aggtgttgtc gtagacttcc acaaccttac cgaccacgcc ttcgggagtg atgacgccca
      721 tatttttctt gatgtgatcc tttgagccgc ggtcgagcat gatagtggcg ctggcggttc
      781 cggcactggt cgcgattacg cgcgcgccaa gcatgggcac gttctcatgg gatttgtgaa
      841 agttgagcag caacgccagg cggtccgcct cgctggcttt gctttggagt tgattgacct
      901 gcatcttgag gtcatcgttt tcgcggtgca gccgctcgtt ttcgagggag gtattttgca
      961 gcgcgaaata gtgacgccac gtttcgcgca agtgcccaat gccatgattg ccggcacgct
     1021 caaagggaga gaacgcgccg acggtccaga cgcgaatcaa gcggccttca gagtcgcgct
     1081 tgatctgcaa ggccagcaac aaaatctgca gcaaaacaac gccggcgagc aggaagatgg
     1141 atttgtgacg agaaggtatg ccggccatgt agttagctga tcaaattccc tgttgctttc
     1201 gaaagctcaa gacttcaaac attcgttcaa aaaactggat cgcgatgggc ttttcgttac
     1261 caaaattctt acactaaaaa agtcaggcat gtgtgacggc cagcgaaaag agctgaggcc
     1321 tcgagcatct tcaatcgatg catacctggc gcagcaaacg gaaatcgctc aacatttttc
     1381 cggtgccaag caccacggat gccagcggat catcggcgat ggaaacgggt aagccggttt
     1441 cttcgcggat gcgcttgtcg agattcttga gtagcgcgcc gcctccggtg aggactatgc
     1501 cgcggtcgct gatatcggcg gaaagttccg gcggggtacg ctcaagggct acgcgaacgg
     1561 cattcaaaat ggtggccacg cattcagaca aggcttcgcg aatttcgctg tcgtcgatgg
     1621 tcaccgtgcg cggcacgccc tcgatcaggt tgcggccctt cacttccatg gtcagcggct
     1681 tttccagcgg gtaggccgat cccagctcca tcttgatctg ctccgcggtg cgctcgccga
     1741 ccagcagatt gtatttgcgc ttgaggtagt gcatcaccgc ctcgtccatc tcat
//
LOCUS       DATCBB010000852         1501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_59666, whole genome shotgun sequence.
ACCESSION   DATCBB010000852 DATCBB010000000
VERSION     DATCBB010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59666"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VII37_09410"
     CDS             complement(<1..1361)
                     /locus_tag="VII37_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY5069998.1"
                     /translation="MKRRIEKVVVLGAGTMGARIAAHFANAGVPVLLLDIVPPGLTSA
                     DAAERNKIVRAGLDAAKKSKPAAFFTAALADKISIGNFEDDLQRCKDADWIIEVVAEN
                     LDIKKNLLARVGQFRKPGAIVTTNTSGLPVHLIAEGMSEEFQQHWAGTHFFNPPRYLK
                     LVELIPGPKTSSEVLETLTEFCDRRLGKGVVVAKDTPNFIANRIGTFSMLNALRLMNK
                     LGMTIEEVDACTGPAIGQPKSATFRTADIVGLDVLMHVVKNIYETATDDESREMYKVP
                     AFVEEMAKRKWLGDKTGQGFYKKLRGEGEKEILTLDINTMDYRPRQKARFASIEAGKT
                     IEDTGDRLRALIGPVLQGQTGDKAQQFLWGSLSETCLYAARRVPEISDRVEDVDRAMR
                     WGFGWELGPFQIMDTLAVPAFAAQIKKEGRKLPPSIEKLLTGGRNSFYTAEKGATSVF
                     ELS"
BASE COUNT          328 a          397 c          403 g          373 t
ORIGIN      
        1 ctacttaatt cgaatacgga ggtggcgcct ttttccgcgg tataaaaact gttgcggccc
       61 ccagtcagca atttttcaat tgagggaggt agcttacggc cttctttttt gatttgtgcc
      121 gcgaaggctg gcactgccaa agtatccatt atttgaaatg gtccgagttc ccagccaaaa
      181 ccccaacgca tggcgcggtc gacgtcttcg acgcggtcgg aaatttctgg gacgcggcgt
      241 gctgcgtata aacaggtctc agagagcgaa ccccacaaaa attgctgggc cttatcgccg
      301 gtttggcctt gcaagacggg gccgataagc gcgcgcaaac gatcgcctgt gtcttcaatg
      361 gttttgccgg cttcgatgga agcgaacctg gctttctggc gcggacgata gtccatggtg
      421 tttatatcga gggtgagaat ttctttttcg ccttcgcctc gcaatttctt gtaaaaacct
      481 tgtccagttt tatcgcctaa ccatttgcgc ttggccatct cttcgacaaa ggcgggcact
      541 ttatacattt cgcgcgattc atcgtccgtc gcggtttcgt aaatattttt aaccacgtgc
      601 atcaggacat ccaggccaac gatgtccgcg gtacgaaatg ttgcggactt cggctggcca
      661 atggccgggc cggtgcaggc atccacttcc tcgatggtca tccccaattt attcatcaag
      721 cgcagggcgt tgagcatgga aaatgtgcca atgcggttag cgatgaagtt tggagtatcc
      781 tttgcaacca ccacgccttt gcccagacgg cgatcgcaaa actcggtgag ggtctccaga
      841 acttcagacg atgttttcgg cccgggaatt aattcaacca gctttaagta gcgcggcgga
      901 ttaaagaagt gcgttccagc ccaatgctgc tggaattctt cgctcatgcc ttcggcgatt
      961 aaatgcactg gcaaccccga ggtattggta gtgacaatcg ctccgggctt gcgaaattgg
     1021 cccacgcgcg ccagcagatt ctttttgatg tcgagatttt cggcgaccac ctcgataatc
     1081 cagtcggcgt ccttgcagcg ctgcaaatcg tcttcaaaat ttcctatgga gattttgtct
     1141 gccagcgcgg cggtgaaaaa tgccgcaggc ttggactttt ttgcggcatc gaggccggca
     1201 cgcacaattt tgttgcgctc cgcggcgtcg gcggaggtca agccaggtgg gacgatatcc
     1261 aacaacagca ccgggacacc ggcgttggcg aaatgagccg cgattcgggc gcccatggtt
     1321 ccggcgccaa ggaccacgac cttttcgata cgacgtttca tgggcggcct tttttctgat
     1381 ttgtacgaac ggacaggtta caaaaccaac atacacgggt caatcgaact gacacaaacg
     1441 ggattagcgc ctagactgat tacagcatgc ctgaaccggt catttcgagc gtgttgcgca
     1501 g
//
LOCUS       DATCBB010000853         1072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_91649, whole genome shotgun sequence.
ACCESSION   DATCBB010000853 DATCBB010000000
VERSION     DATCBB010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91649"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VII37_09415"
     CDS             complement(<1..665)
                     /locus_tag="VII37_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY5069999.1"
                     /translation="MLAVATVRIALVTHRILAPTDLVIIGIYFAIIFGIGFYFAKKGR
                     TSEDYFLASRNIGWFAIGASLFVSNVSTEHFIGLAGSGATSGLAVGDFEWLACMMLLL
                     LGWVFVPFYLRSNVFTMPEFLERRFNRNCANYLATISVIAYIFTKISVHLYAAGIVLE
                     RVVGWNQWTAAIILVIATGVYTIAGGLAAVIYTDLVQTLILLAGAIALTLIGLDKVGG
                     FGG"
     gene            840..>1072
                     /locus_tag="VII37_09420"
     CDS             840..>1072
                     /locus_tag="VII37_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070000.1"
                     /translation="MSKLLCCILLLGASVMAADKNVYEFTLNSIDGQSAPLAAYKGKV
                     VMLVNVASRCGFTPQYSALEAVYEKYKDRGFVIV"
BASE COUNT          306 a          269 c          288 g          209 t
ORIGIN      
        1 aagccgccaa atccgccaac tttatccaag ccgattaacg tgagcgcgat cgcgcccgcc
       61 agaagaatca gcgtctgtac taaatccgtg tagatcaccg cggcgagacc gccggcgatg
      121 gtatagacac cggtggcaat caccagaata atggccgccg tccactgatt ccaaccgacg
      181 acgcgctcca gcacgatgcc cgcggcataa agatgcacgg aaatttttgt gaagatataa
      241 gcgatcacgg aaatagtggc cagatagttc gcgcaattgc gattgaaacg gcgctccaga
      301 aattccggca tcgtgaatac attggagcgc agataaaaag gcacgaacac ccagccgagc
      361 agcagcaaca tcatgcatgc cagccactca aaatcaccca cggccaggcc agaggtggcg
      421 cccgatcccg cgagacctat gaaatgttcg gtggaaacat tggaaacaaa aagcgaggcg
      481 ccgatcgcga accaaccgat attacggctg gccagaaaat aatcctcaga ggtgcgtccc
      541 ttttttgcaa aatagaatcc gatgccaaaa ataatggcga aataaattcc tataattacc
      601 aggtccgtgg gtgcgagaat gcgatgcgtt actaatgcga ttcttacggt cgctaccgcc
      661 agcatcgcac ctcctcgtgc agaataggga aataaacgac tggcctgcgg agtctataac
      721 aggggcagcc tacgctcaat aacggattgg cggattggcg acaaatcgca gcaagccaag
      781 accgcagcaa tcatttgcgg ctacggctgc aacggagtaa aaacatctaa gggggaaata
      841 tgagcaagct gttgtgttgc attcttcttc tgggggcgag cgtgatggca gcggataaga
      901 acgtctacga atttactttg aattccatcg acgggcagtc agcgccgcta gcggcgtaca
      961 aaggaaaagt ggtgatgctt gtgaatgtgg ctagccgttg cggtttcacg ccacaatatt
     1021 cggcgctgga agcggtatac gaaaagtata aggatcgtgg ctttgtgatt gt
//
LOCUS       DATCBB010000854         1364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_91728, whole genome shotgun sequence.
ACCESSION   DATCBB010000854 DATCBB010000000
VERSION     DATCBB010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91728"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VII37_09425"
     CDS             complement(<1..845)
                     /locus_tag="VII37_09425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5070001.1"
                     /translation="MPIEHHRPLDSPISASSERVTAPSDRAAPCAGGITAIVPARDEE
                     AVIAACVESLAKQQELTDVVVVNDQSSDQTGAIVRDLATRIAKLQLLETQAPPPGWVG
                     KNNALVFGARNATSDWLLFTDADVELEEGAMTRALQIAEENRAELVSLSPPQITEKWY
                     EKALIPFVFCRLARRFSYAAVNDPKSAAAAANGQFLLIKREVYEAIGGHARFASDVLE
                     DVALATAVKAAGYRLWFGSGKGIVRARMYRTFGAMWEGWTKNLYRLMGGTPWEAFREG
                     ESAIP"
     gene            899..>1364
                     /locus_tag="VII37_09430"
     CDS             899..>1364
                     /locus_tag="VII37_09430"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5070002.1"
                     /translation="MPLVRRIAGWAAVAFAAGILVLFAMPSYRQGEASIAGKSAVDFP
                     LTLAGKSERLSDLKGKVVVLNFWATWCPPCVEETPSLNRLQKYIDSRGGMILGVSIDE
                     DGAAYEQFLRDQNVVFPTYRDATKKTASDYGTSIFPETYIIDRHGKIARKFVG"
BASE COUNT          292 a          420 c          353 g          299 t
ORIGIN      
        1 cagggaatcg ccgactcgcc ttcgcgaaac gcctcccatg gcgttcctcc catcaagcga
       61 tagagattct tagtccatcc ctcccacatc gcgccaaacg tgcggtacat tcgcgctctt
      121 acaattccct tccctgagcc gaaccacaaa cggtacccgg cggcctttac cgccgtggcc
      181 agcgccacat cttccaaaac gtccgaggca aatcgcgcgt gcccgccgat cgcttcgtag
      241 acctcgcgtt ttatcagcaa gaattgtccg ttggctgcag ctgccgcaga cttcgggtcg
      301 ttcacagcgg cataggaaaa ccgtcgcgcg agcctgcaaa atacaaacgg tatcaacgct
      361 ttctcgtacc acttttccgt gatttgcggc ggcgacaaag aaaccagctc cgcgcggttt
      421 tcttcagcaa tctgtaacgc acgagtcata gctccttctt cgagttccac gtccgcgtcg
      481 gtaaacaata gccaatcact cgtggcgttg cgggcgccaa acaccagcgc attgttttta
      541 ccaacccaac cgggcggggg cgcttgcgtt tccagcaatt gcaacttggc gatgcgtgtg
      601 gccaggtctc ggacgatggc gccggtttgg tccgacgatt ggtcattcac gaccacgaca
      661 tcggttaact cctgctgctt cgccagcgac tcaacacacg cggcaatgac cgcctcttca
      721 tcgcgcgccg gcacaatcgc ggtaattccg ccagcgcagg gcgccgccct gtcagatggc
      781 gccgttacac gctcactgct cgccgaaatg ggcgaatcca gcggtctatg gtgttcaatt
      841 ggcattcgtg aaggtcgaac gcagccccgt acgttgaaga taggttatta tagcgggcat
      901 gccactagtg cgaagaatcg ctggctgggc tgctgtggcg tttgccgcag gcattctggt
      961 ccttttcgcc atgcccagtt accgccaagg tgaagcgtcc atcgcgggca agtctgccgt
     1021 ggacttcccc ctcacactcg ccggcaaatc cgagcgtctc tccgatttaa agggaaaagt
     1081 agtagtgctg aatttctggg ccacctggtg tccgccttgc gtggaagaga cgccttccct
     1141 taatcggctg caaaaatata tcgattcgcg tggtggaatg atcttaggcg taagcatcga
     1201 cgaagatggc gccgcgtatg aacaattctt gcgcgatcaa aatgtagtgt ttccgacata
     1261 tcgcgatgcc acaaaaaaga ccgcgtctga ttacggcact tcgatattcc ccgaaacgta
     1321 catcatcgat cgtcacggca aaatcgcccg caaattcgtc ggcg
//
LOCUS       DATCBB010000855         1351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_157890, whole genome shotgun sequence.
ACCESSION   DATCBB010000855 DATCBB010000000
VERSION     DATCBB010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157890"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..697
                     /locus_tag="VII37_09435"
     CDS             86..697
                     /locus_tag="VII37_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY5070003.1"
                     /translation="MSISKEDHERIARAIHSAEAKTSGQIVCVLAQASSHTTALPVFI
                     AAVASLVMPWLLITFTAMTVHRILSLQSVVFLALLVLFCLPRVRIALMPRKARRAIAH
                     RAAMEQFNSRGIGQRKDRSGILIFVSLAERYARIIADDEIAARVAQSEWQAAVNALVA
                     HMRDGRIADGFVTAINLCGNELAKHFPRTATSNNAQPDRIYLI"
     gene            853..1029
                     /locus_tag="VII37_09440"
                     /pseudo
     CDS             853..1029
                     /locus_tag="VII37_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023098450.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          286 a          393 c          394 g          278 t
ORIGIN      
        1 gcggccgcgg cggattttcg ggtggcggca gtttttcagg aggtgggggg tcgtcaggtg
       61 gcggcggggc ttcggggagc tggtgatgag catttcgaaa gaagatcacg aacgcatagc
      121 gagggccatc cattctgccg aggcaaagac ctcgggccaa atcgtttgcg tgctggcaca
      181 ggcatcctcc cacaccacgg cattgccggt cttcatcgcc gcggtagctt cgctcgtgat
      241 gccctggttg ctgatcacct tcacagcgat gacagttcac cgcattctat cgctgcagag
      301 cgtcgtattt ttggcgctat tagtgctttt ctgcctaccg cgcgtacgga ttgccctaat
      361 gccacgtaaa gcgcgccgcg ccatcgctca ccgcgctgcc atggagcagt ttaacagccg
      421 tgggattggg caacgaaaag atcgctctgg gattctgatc ttcgtctcgc tcgcagagcg
      481 ttatgcgcgc atcattgccg acgatgaaat agctgcacgt gtggcccaat ccgaatggca
      541 agcggccgtt aatgcgctgg ttgcccacat gcgtgatggc cgcatcgccg atggctttgt
      601 tacggcgatc aacttgtgtg gaaatgagct tgcaaagcac ttcccacgca ccgcgacgag
      661 caacaatgcg cagccggacc gaatttacct catctgacgc caacgttgtc aggtcggtgt
      721 catccgcgct gactttgcgc tcaatggtct ggttcgtttg cccggaattc taccgaaacg
      781 ttctgagccc ccgctaggcg gggagcgatt gctcgcgacg tgatgtgaat ttaaaaaaga
      841 gtgccaaatc ccgagacggt tgagcatccc ttcggcacga tcaaggccag gatgggcgca
      901 acccacttcc tgatgaagac gctgccacgg gttgccgccg agatggcgtt gcacgtgctg
      961 gcctataacc tcacgcgcgt catgaacatc atgggcacca aaccgctcat ggcggcgatg
     1021 agggcatagc cgccgccgac atgcgcccgc ctgccattgt agaaccgccc acgaccgctg
     1081 caatccgcat cagccgcgcc tacagtcgca aaagccgtca aatccaaaaa taatgaccgc
     1141 gcatggctac ctagccgagt tcgcgccgcg tctatgcctc tgtcaggcgt tttcacacga
     1201 ccaagaccca aagcagacat caaaagggcg ccgctcgcgc ttacaaagtt ccatcgcgat
     1261 aacgtgtgta cagcaatagg aacgtgagag gcgaaggcga gttggcggga cgggggcaga
     1321 cggtatggtt tatggatgcc ctattcacaa t
//
LOCUS       DATCBB010000856         1577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_351456, whole genome shotgun sequence.
ACCESSION   DATCBB010000856 DATCBB010000000
VERSION     DATCBB010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351456"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..>1577
                     /locus_tag="VII37_09445"
     CDS             253..>1577
                     /locus_tag="VII37_09445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5070004.1"
                     /translation="MTTVQREQLEADVLIVGAGPAGLACALHLANVIKKHNEAGGAAG
                     GNTLSAENIYVLEKGREVGAHQLSGAIMNPKALAELVPDYKNSAPLDTPVTDDAALFF
                     TKSSSIRFPLTPPPFKNKGNYVVSLNKIAKWMGGLVEAAGVNLFTQFGGASLIYEGDG
                     IAGVITEDKGVDKNGKPKANFTPGYELRAKVTVLAEGTRGSLTKDLVEKKNLANINPQ
                     TYGLGIKELWDVQPGRIQPGFVAHTLGWPISTDMYGGGWIYGLSNNRVYIGLVVALEY
                     GDPLFDPHAAFQQWKTHPFLKKILDGGKLVRYGAKSLPYGGWYAMPRNYLDGALIIGD
                     SGSFLDSQRLKGIHLAMKSGMLAAETIFDALKVGDTSAKMLSAFPKKVEQSYIKKELW
                     QVRNFHQSFQHGLLPGLFQTGLQFISGGRGLVDPMRNHAGYEAYKKLNGS"
BASE COUNT          389 a          413 c          433 g          342 t
ORIGIN      
        1 atattcttgc gagccgtacc cgcgctaccg gcggccttat ccttctcgat cgtgacggaa
       61 atcctggatg ggcttttaat actccgcgca tgtcctacgg ctatgtccaa gacgatggca
      121 ggatcgtcac cgcggtgtag cctttatggc aattttatcg agcaaaccgg gagcaggacc
      181 gaacaaagaa ttatcgtttt ccatcccatg ccgtcgtaac aaggctggta acccatttgc
      241 tagaatgaag gaatgacgac cgtacagcgc gagcaacttg aggcggacgt tctcatcgtt
      301 ggcgccggac ctgcggggtt ggcctgcgcc ttacatctcg cgaacgttat caaaaagcac
      361 aatgaagccg gcggcgcggc gggcgggaac acgctctcgg cggaaaatat ctacgtactg
      421 gaaaaaggac gagaggttgg cgctcaccag ctttccggcg cgatcatgaa tcccaaagca
      481 ctagctgagc tggtgccgga ttacaaaaac tccgctccgt tggacacccc ggttaccgat
      541 gacgcggcat tgttttttac caagagctct tccattcggt ttccattgac tccgccgcca
      601 ttcaaaaaca agggcaatta tgttgtttcc ctcaataaaa ttgcgaaatg gatgggcggg
      661 ctggtggaag ctgcgggcgt aaaccttttt acgcaatttg gcggcgcttc gttgatctat
      721 gaaggcgatg ggatagccgg cgtaatcacg gaagataagg gcgtcgataa aaacggcaag
      781 cctaaagcca atttcacccc cggatacgaa ttgcgcgcca aagtaacggt attggccgag
      841 gggacgcgcg ggtctctgac gaaagacttg gtggagaaaa aaaatctcgc caacatcaat
      901 ccgcagactt acggcctggg catcaaggaa ttgtgggacg tgcagcccgg ccgcatccaa
      961 ccgggtttcg tggcgcacac gctgggctgg ccaatttcca ccgacatgta tggcggcggg
     1021 tggatctacg ggctatcgaa taatcgcgta tacatcggac tggtggtggc actcgaatat
     1081 ggggatccgc tattcgatcc gcacgcggcg tttcagcaat ggaaaactca tccgttcctc
     1141 aaaaaaatcc ttgatggcgg gaagctggtg cgttacggcg caaaatcgtt gccctacggc
     1201 ggctggtacg ctatgccgcg aaattatctg gatggcgcgc tgatcattgg agattccggc
     1261 agtttcctcg attcgcaaag actcaaagga atccacctgg cgatgaaaag cggcatgctg
     1321 gccgcagaga caattttcga cgcgttgaaa gtcggcgaca cgtcggcgaa aatgttgagc
     1381 gcatttccca aaaaagttga acaaagctac atcaaaaaag agctgtggca agtccgcaat
     1441 ttccatcaat ccttccagca tggtttgctc cctggattgt tccaaactgg cttgcaattc
     1501 atttcgggtg gccgcggcct cgtcgatccg atgcgcaatc acgccggcta cgaagcctac
     1561 aaaaaactta acggctc
//
LOCUS       DATCBB010000857         1531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_351504, whole genome shotgun sequence.
ACCESSION   DATCBB010000857 DATCBB010000000
VERSION     DATCBB010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351504"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VII37_09450"
     CDS             complement(<1..738)
                     /locus_tag="VII37_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEY5070005.1"
                     /translation="MNSAMAQARSKISADATDAKITANAAERYWRATLARDTRADGTF
                     ILGVLSTRIYCRPSCPARRPLRRNVVFFGTGEEAEKQGFRPCLRCKPNEKSGALQLAE
                     RAARELANGEEEILRVATLAGKLGVTPSALRRAFQQVTGLAPRDLADALRLNRFKNLL
                     RAGRNITDALYETGYGSSSRVYERSDAQLGMTPAAYQKGGKGMKLEYTIAKSPMGKVL
                     VAATEKGVSAVYLGEAEQKLIGELREEY"
     gene            908..1219
                     /locus_tag="VII37_09455"
     CDS             908..1219
                     /locus_tag="VII37_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070006.1"
                     /translation="MTAHQINRFSAKVIIVLSVLALLTVLSGYTHPPQAPEPDEGAAA
                     HIFQLSIAAVALSILVFLSTADWKQPSRSVRPLAFPAAALVLAFGALYYLEHFRYIAA
                     H"
     gene            complement(1228..>1531)
                     /locus_tag="VII37_09460"
     CDS             complement(1228..>1531)
                     /locus_tag="VII37_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5070007.1"
                     /translation="ELHFAAISGRSWMALIYLVVLGSGVGFTAYLYILEKSTPSKVAT
                     YAFVNPVVALFLGWMIVGETITPRTAIAAAVILTAVILVISAPRAAAAKSEVQNQE"
BASE COUNT          334 a          517 c          330 g          350 t
ORIGIN      
        1 atattcttct cgcagttcgc caatcaattt ctgttccgcc tcgcccagat aaaccgcgga
       61 aactcctttt tccgtagccg ccaccaaaac tttgcccatc ggtgatttcg caatcgtgta
      121 ctcgagcttc ataccctttc ctcctttttg ataagccgcc ggcgtcatcc ccagttgcgc
      181 gtcgctgcgt tcataaacgc ggctcgaaga gccatagccc gtttcataca acgcatcggt
      241 aatatttctt cccgcacgca acaaattctt gaagcgattc aatcgcagcg catccgccaa
      301 gtcccgcggc gccagtccgg taacttgctg aaaagcgcgt cgcaacgcgg acggcgtaac
      361 gccaagtttt cccgccagcg tagccacccg aagaatctct tcctcgccgt tcgctaattc
      421 tcgcgccgcg cgttccgcca actgcagcgc tcccgatttt tcattaggct tgcaccgcaa
      481 gcatgggcga aacccttgct tctccgcttc ttcaccagtg ccgaagaaaa ccacgtttcg
      541 ccgcaacggc cgccgggccg ggcacgatgg ccggcaataa attcgcgttg aaagcacgcc
      601 cagaataaat gtcccatccg cgcgagtatc acgcgccagc gtcgcgcgcc aatagcgttc
      661 cgccgcgtta gccgttatct tcgcgtccgt cgcgtcagca gatatcttcg acctcgcttg
      721 tgccatcgca ctattcatat tgcactcgtc aaaactacct cactgcaaaa aacaaagcta
      781 tccgctaacg cgcaccaaag ccacccgcga caagaatgaa tttttccact tgaccacgga
      841 actttttgcc tttatattgc aaagtacttt ctactgcggt caacagccaa ctgaaaggag
      901 ctgccccatg actgcgcacc aaatcaaccg tttcagcgcc aaagtcataa tcgtcctgtc
      961 tgttttagct ttgcttacgg ttcttagcgg ctacactcat ccgcctcaag ctcccgaacc
     1021 ggatgagggc gcggccgccc acatctttca gctgtccatc gcagccgtag cactctcgat
     1081 cttggtcttt ctcagcaccg cggattggaa gcagccctcg cggagtgtcc gcccattagc
     1141 atttcctgcg gctgctttag ttcttgcgtt tggcgcgctc tattatcttg aacactttcg
     1201 atacattgcg gcgcactaac ctgccgccta ttcctggttt tgcacttcgc ttttagccgc
     1261 cgcagcgcga ggcgcgctaa tcaccaggat caccgccgtt agaatcaccg ccgccgcaat
     1321 cgccgtccgc ggcgtgatcg tttccccaac tatcatccaa cccagaaaaa gcgccaccac
     1381 cggattaaca aaagcatacg tcgccacttt cgagggcgtg cttttttcca gtatgtacag
     1441 gtaagccgta aacccaaccc ccgagcccag caccaccagg taaatcagcg ccatccacga
     1501 acgccccgaa atcgccgcaa agtgcagctc a
//
LOCUS       DATCBB010000858         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_188781, whole genome shotgun sequence.
ACCESSION   DATCBB010000858 DATCBB010000000
VERSION     DATCBB010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188781"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VII37_09465"
     CDS             <1..1360
                     /locus_tag="VII37_09465"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY5070008.1"
                     /translation="DSERNCYATLGVVHHIWHPVPGRFKDHIAMARPNLYQSLHTTVL
                     HGGQPLEVQIRTQEMHRIAEEGVAAHWKYKGGKPGSEDENQRLTWMRQLIEWSQALEE
                     PGDFLSTLKVDFAPVEVYAFTPKGRVLELPRGATPVDFAYAVHTEVGHQCTGAKVNGQ
                     MVSLRHEITSGDVVEILTQKGHHPNRDWLSFVKSSHAKSKIRHWINLEEREEATEMGR
                     KLLENEARHFDRSLKKIPEADLLKVATDHGLSKIDDLYAAVGFGKYSARQILTRVLGE
                     PAKATQVEAPDAKPTLVKTVKRMLGFGEAPLIVKGHDDLLVYRAKCCNPIPGDEIVGY
                     ITRGRGVAVHTRTCPNVQNLLYQTERRIAVEWGGENAATFPVELQIRARDRAGLLADI
                     TAVISGAGSNIRNLVSRPDGVNARIDASLEIVDRRQLETILVNIRKIAAVYGVERVYQ
                     TS"
     gene            1481..2161
                     /locus_tag="VII37_09470"
     CDS             1481..2161
                     /locus_tag="VII37_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070009.1"
                     /translation="MTLHGNSFPWPARVRTVAAPAVSLNLSLLAVVIACTLAISSGCD
                     RHLLTDYRPLVNAGMSSASIEQLKKLDTSNPETLQLVSAKQAGIPDYTCVTLVSDAHQ
                     HHHLFASAEAVISLAGAGFSEQQVLQIAAADKLDTLSGDAVTLRLIGLSDSMVQTVLQ
                     RRLQDQPTLSSPEIARLKNTGLTEAQILQRINRGMDDTEADKEVLTRETVRNHNNTGF
                     VRIRGRRR"
     gene            2268..3794
                     /locus_tag="VII37_09475"
     CDS             2268..3794
                     /locus_tag="VII37_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070010.1"
                     /translation="MKLLSNLRALSILFLVASWSFVAGCTSSFGPGYTINKQDIDVHF
                     EPGPPPHIAIKSSYELTNNGTLPLFVVELRLPAKHRFKSSNVELTWDGQAISSEPSPD
                     YPRNTLLKLPQPWTVSARHSLHIAMNLETPAPDEQSYLGFAPDAFFLPAEGWAAELLP
                     AKGIFATGGAPPDKWNLTLHVPQGFVVHTSGDKIKTSKHGDETTVQARQRIVDIYPSV
                     IAGRYVTKQIGSDRQKIDLWTRKPQDPGDIRAVSDSLIKTLANFNLVFGARSLATPPT
                     GFFGRHPTPGKNGLPLWLAECPVIPGCFTERNPLNAQLLGTKDQGRSGEMISLDSAMI
                     DPGPGLKKMALATAPALAASWLGYGQNPGFYEQDPPLSAFPAFAAAVGDEALNGAAER
                     IETIRRALALVANNSSPAAKEDGAALYAKSFLFFYALQDRYGQETFRKAVRHMLDARR
                     NTGFNLDDLIAAFDEEAHGNTAAFVRLWMKHPGVPADFRAKYENTSAQKNTPLKETTP
                     "
BASE COUNT          897 a         1181 c         1032 g          807 t
ORIGIN      
        1 cgacagcgaa cgcaattgct atgcaaccct cggagtcgtg catcacatct ggcatccggt
       61 ccctggacga ttcaaggatc acatcgccat ggcccgcccg aatctttacc aatcgctgca
      121 caccaccgtg ctgcatggcg gccagcctct tgaagttcag attcgcaccc aggaaatgca
      181 tcgcatcgcc gaagaaggcg tcgccgcgca ctggaaatac aaaggcggga agcccggctc
      241 cgaggatgaa aatcaacgcc tcacctggat gcgccagttg atcgaatggt cgcaggcgct
      301 ggaggagccc ggcgatttct tatccacgct aaaagtagat ttcgcgcccg tggaggttta
      361 tgccttcact cccaagggcc gcgtgctgga gctgccgcgc ggggccactc cggtggattt
      421 cgcctatgcc gtgcacaccg aagtcggcca tcaatgtacc ggcgcgaagg ttaacggcca
      481 gatggtctcg ctgcgccatg aaatcaccag cggcgatgtt gtggagattc tcacccagaa
      541 aggccaccac cccaaccgcg actggctgag tttcgtaaaa agctctcacg ccaaaagcaa
      601 aattcgccac tggatcaatc tcgaagagcg cgaggaagcc accgagatgg ggcgcaagct
      661 gcttgaaaat gaagcgcgcc actttgatcg cagcctcaag aaaattccgg aagcggattt
      721 gttgaaggta gctaccgatc acggcctttc aaagattgac gatttgtatg ccgcggtagg
      781 ctttggaaaa tattcagcgc ggcagattct gacccgtgtg cttggtgagc cagccaaggc
      841 cacgcaagtc gaagcgccgg atgctaagcc cacgctggtt aaaaccgtca agcgcatgct
      901 gggtttcggc gaagcgccgc tcatcgtcaa agggcatgac gatttattgg tctatcgcgc
      961 gaagtgctgc aaccccattc ccggtgacga aattgtcggc tacattacgc gcggccgcgg
     1021 tgtcgcggtc cacacgcgca catgtcccaa tgtgcagaat ctgctatacc agaccgagcg
     1081 tcgcatcgcc gtggaatggg gtggcgagaa tgccgcaacc ttcccggtgg agctgcaaat
     1141 ccgcgctcgc gatcgcgctg gtttgctcgc cgacatcact gcggtgatca gcggggccgg
     1201 ttcgaacatc cgtaacctcg tcagccgccc ggatggcgta aacgcacgca ttgatgccag
     1261 cctggaaatc gtggatcgcc gccaactgga gaccatcctg gtaaatatcc gcaagatcgc
     1321 cgctgtttac ggcgttgagc gcgtttatca aaccagctga attctcaaat ttggcgattg
     1381 cgcgaacgca cttcctacct gtcctttagg cgggtatctc ccactacaca ccttcccctt
     1441 gccaccggtt agcgcctgga cgcgcgaata caattgaatc atgactcttc acggcaattc
     1501 gttcccatgg cccgcacgcg tgcgaaccgt ggccgctccg gcggtgtcat tgaacctgtc
     1561 gctgctggcg gtagtcatag cctgcacttt ggccatttca agtggctgcg atcgtcacct
     1621 gttgaccgat taccggcccc tggtgaacgc gggaatgtct tcagcaagca ttgagcaact
     1681 gaaaaaattg gacacctcga accccgaaac gctacagctc gtgagcgcta agcaggcggg
     1741 aatcccggat tacacctgcg tcacactggt aagtgatgcg caccagcatc accacctttt
     1801 tgcaagcgcc gaagcggtca tcagcctggc tggggctggc ttcagcgagc agcaggtttt
     1861 gcagatcgcc gccgccgata aactcgatac tcttagcggc gatgccgtga ccttgcgtct
     1921 gatcgggctg tcggattcca tggtgcaaac tgtgttgcaa cgccgccttc aggaccagcc
     1981 gaccctttcg agcccggaga ttgctcgttt gaaaaatacc ggcctgacgg aagctcaaat
     2041 ccttcaacgc atcaatcgtg gaatggacga caccgaagcg gacaaagaag tattgacgcg
     2101 cgagacggtc cgaaatcata acaataccgg ttttgttcgc atccgtggcc gtaggcgtta
     2161 aaacgttctg tcgccagcat cgtcctaagc caaaatggcg cggctaacgc aatttcgttc
     2221 acgaactcgc cttttgccgg agttgaatcc tcgctacaat cgaagcgttg aaactccttt
     2281 ctaaccttcg cgcattatca attctttttt tagtcgcgag ctggagtttt gtcgctggat
     2341 gcacgtcatc cttcggtccc ggatacacca tcaacaaaca agacatcgat gttcattttg
     2401 agccgggccc gccgccgcat atcgcgatta agtccagcta cgaactcacc aataacggca
     2461 cactgccact tttcgtcgtg gagctgcggc ttcccgcaaa acaccggttc aaatcctcaa
     2521 acgttgagct cacctgggat ggacaagcaa tcagttctga accgtcacct gactacccac
     2581 gcaatacact tttgaagtta ccgcagccct ggacggtatc cgcccggcac agcttgcata
     2641 tcgcgatgaa tttggagacg cccgcaccgg acgagcagtc ttatttgggc ttcgcgcccg
     2701 atgccttctt cctccccgcc gaaggatggg ccgcggaact tttgcctgcc aaaggaattt
     2761 ttgccactgg cggcgcgccg ccagacaaat ggaatctcac cctgcatgtg ccccaggggt
     2821 ttgtcgtcca taccagcggc gacaaaataa aaacttccaa gcacggagac gaaaccaccg
     2881 tccaagccag gcaacggatc gtcgacattt atccttccgt gattgccggc cgctacgtca
     2941 ccaaacagat cggttccgac cggcagaaaa ttgatttatg gactcgcaaa ccacaggatc
     3001 ccggcgatat tcgcgcagtg agcgattcgc tgataaaaac tctagcgaat ttcaacttgg
     3061 tgtttggcgc ccgctcgctc gccactccgc caacaggttt ctttgggcgt catcccacgc
     3121 ccggaaaaaa cggtttacca ttgtggctgg cggagtgccc ggtcatcccg ggatgcttca
     3181 ccgagcgaaa ccctctcaac gcccaactgc tcggcactaa agaccaaggc cgaagcggtg
     3241 agatgatctc gcttgacagc gccatgatcg atccaggtcc aggactaaaa aaaatggcgc
     3301 tcgcaacggc ccccgcgttg gccgccagct ggttaggtta tggccagaac cccggttttt
     3361 acgagcagga tccgcccctg tcagcttttc cggcatttgc cgcggccgtt ggtgacgaag
     3421 cactgaacgg cgcggccgag cgcatagaga cgattcgccg cgcgctcgcg ctggtagcga
     3481 acaactcttc gcccgcagca aaagaggatg gcgccgctct gtacgcgaaa agttttctgt
     3541 tcttctacgc gctccaggac cgctacgggc aagaaacatt tcgcaaggcc gtcaggcata
     3601 tgctggatgc ccggcgcaac accggcttca atctagacga tttgatcgcc gcgtttgacg
     3661 aagaggcaca cggcaacacc gctgcgttcg tccgtctgtg gatgaaacat cccggagtgc
     3721 ctgcagactt tcgcgcgaaa tatgaaaaca cctccgcgca gaaaaacaca cctttgaagg
     3781 agactacgcc atgaaagata tcgttcttac cgaccgagga ccgaaaccta tcggccccta
     3841 ctcacaagga gtacgcgcta acggattttt attcatttcc ggccaggtag cctttgatcc
     3901 agccacctcc gaaatca
//
LOCUS       DATCBB010000859         1314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_416988, whole genome shotgun sequence.
ACCESSION   DATCBB010000859 DATCBB010000000
VERSION     DATCBB010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416988"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..453)
                     /locus_tag="VII37_09480"
     CDS             complement(103..453)
                     /locus_tag="VII37_09480"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5070011.1"
                     /translation="MPNNELTRRYPRVKAPEGILVGWQVGTKRTVSHLEDIALGGLFI
                     RTTDPPPERSLVKLVLSAGTAEVRARGVVRRVAPHQGMAIEFTAMNPDDRGRLTGLLR
                     PLLERQGAGTERPI"
     gene            complement(514..1053)
                     /locus_tag="VII37_09485"
     CDS             complement(514..1053)
                     /locus_tag="VII37_09485"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY5070012.1"
                     /translation="MSISLGQGVTLTKPDDFGKLLEDEGVIFAQLDKAVNWARRSSIW
                     PLGFGLACCAIEMMSMAAARFDVARFGAEVFRPSPRQADLMIVAGRLSQKMAPVIRRL
                     YDQMPEPKWVISMGACATSGGVFNNYAIVQGCNQVIPVDVYVPGCPPRPEALLYSILQ
                     LQKKIDAETGSFKRALNLD"
     gene            1114..>1314
                     /locus_tag="VII37_09490"
     CDS             1114..>1314
                     /locus_tag="VII37_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070013.1"
                     /translation="MKYDQGPTSLHAKKQRLDARSHPAVYSRDMFYYVVKSNLANAAG
                     RVVGYCGSGAVQPTVCETFAEVR"
BASE COUNT          295 a          335 c          408 g          276 t
ORIGIN      
        1 gaaggtacag tggagcggcg gcagcgcaac cgttgtcgag tttgccgaca cgctccatcg
       61 atgaggttca aaggcaaatt ttccgttgcg ggaatgcgga ggctaaatgg ggcgttctgt
      121 cccggcgcct tggcgttcta agagtgggcg cagcaaacct gtgaggcggc cacggtcgtc
      181 gggattcatg gcggtaaact cgatggccat tccctgatgt ggagctacgc ggcggacgac
      241 accacgcgcg cggacttcag cggttcctgc ggatagaaca agtttaacca ggcttcgttc
      301 cggaggcgga tcggtagtgc gaatgaaaag tccgccgagg gcgatatctt ccaggtggga
      361 gacggtgcgt ttggttccta cctgccaacc gacaagaatg ccttcgggtg ctttgacgcg
      421 cggataacgt cgtgtgagtt cgttgttcgg cattccccaa gcccgactgc tggcgtgacg
      481 cagagcgctg agctaggggc agcgcgaaat gcgtcaatcc aaatttaaag cgcgcttgaa
      541 ggaacccgtt tccgcatcaa tctttttttg cagctgcaga atcgagtaca agagcgcttc
      601 cgggcggggg ggacaccctg gaacgtaaac atccactgga ataacctgat tgcaaccttg
      661 cacgatggcg taattattaa agacgccgcc ggatgtcgcg caggcgccca tggagatgac
      721 ccacttgggc tcgggcattt gatcgtacag gcggcgaata acgggagcca ttttttgtga
      781 gaggcggcca gcgacgatca tgagatcggc ttgacgtggg gaggggcgga aaacttccgc
      841 gccgaatcga gcgacgtcaa aacgggcggc ggccatggac atcatttcta tagcgcagca
      901 agccaagccg aaaccgaggg gccagatgga gcttctgcgc gcccagttta cagctttgtc
      961 gagctgggcg aagattacgc cttcgtcttc gagcagcttg ccgaaatcat cgggcttggt
     1021 gagtgttacg ccttggccga gagatatgct catgatgcct cgtcacaact atattcgcac
     1081 atttttgttt gcgaggacaa attgcagttg cgcatgaaat acgatcaagg tccaaccagc
     1141 cttcacgcta aaaaacagcg gctggacgct cggagccacc cagccgttta tagcagagac
     1201 atgttctatt acgtggtcaa aagcaacttg gccaacgcag ctggccgagt tgttggttac
     1261 tgcgggagtg gtgcagtgca acccacggtt tgcgaaacat ttgccgaggt cagg
//
LOCUS       DATCBB010000860         1661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_448189, whole genome shotgun sequence.
ACCESSION   DATCBB010000860 DATCBB010000000
VERSION     DATCBB010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448189"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..572
                     /locus_tag="VII37_09495"
     CDS             147..572
                     /locus_tag="VII37_09495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5070014.1"
                     /translation="MSDDTILAPNPENKCFGCGGANDGGMKLTFTQDNAGRRIVGRFV
                     LGPRYQGGGGMGHGGIIATLLDEAMGKVCRFREARAVTAEMSIQYLKPVRVDEEIIVE
                     AHETNFVGRNIFQSGEIRNAAGDVLARGTARFVIIAPKQ"
     gene            677..1597
                     /gene="pdxS"
                     /locus_tag="VII37_09500"
     CDS             677..1597
                     /gene="pdxS"
                     /locus_tag="VII37_09500"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY5070015.1"
                     /translation="MDAKKVNGSANGNVGAKEWRVKVGLAEMLKGGVIMDVTTADQAR
                     IAEKAGACAVMALERVPSDIRKEGGVARMASIKVIREIMATVTIPVMAKVRIGHFAEA
                     DVLQALEVDFIDESEVLTPADEENHIWKHDYRVPFVCGARNLGEALRRIAEGAAMIRT
                     KGEAGSGNVVEAVRHMRTIVSQMKKLTTLRDDELMHEAKELGAPIDLIRQIAKTGKLP
                     VPNFSAGGIATPADAALVRRLGAEAVFVGSGIFKSSDPEARAKAVVKATTHFDDPKAV
                     LEAHEELGEAMKGLDIRQMEEKDMMSTRGW"
BASE COUNT          388 a          316 c          619 g          338 t
ORIGIN      
        1 ggatgaaaag cagatcgcgc ggggagtggt gacgctggcg gagatgttga aggtggagat
       61 gcgcaagcga cagcgaggtg tacgacgagc ggagcgcagc cgagtggcgt tagtgtgaat
      121 tcttggctgg ggaaggcttg ggttaattga gcgacgatac aattttggcg ccgaatccgg
      181 aaaataaatg tttcggatgc gggggcgcga atgatggcgg gatgaaattg acgtttacgc
      241 aggacaatgc cgggcggcgg attgtcggac gatttgtgct ggggcctcgg tatcagggcg
      301 ggggtggcat ggggcatggc ggaattatcg ccaccctgct cgatgaagcg atgggaaaag
      361 tttgccggtt tcgcgaagcg cgggcggtga cggcggaaat gtcgattcaa tatttgaagc
      421 cggtgcgggt ggatgaggaa atcatcgtag aagcgcatga gacgaatttt gtggggagaa
      481 atatttttca gagtggggag attcggaatg ccgcggggga tgttttggcc cgcggaacag
      541 caagatttgt aattattgca ccaaagcaat aattacgacg ttcgaagttc gagattcgag
      601 gattcaaagg cgagattcaa gatattgatg ccgattgtta aggccatcta aattatcggg
      661 agcgagagga gcagttatgg acgcgaaaaa agtgaatggt agcgccaacg gaaacgtggg
      721 agctaaagaa tggcgcgtga aggtggggct ggcggagatg ctgaaaggtg gcgtgatcat
      781 ggacgtcact accgcggatc aggcgcggat tgcggagaag gccggcgctt gcgcggtgat
      841 ggcgctggag cgggtgccct cggacattcg caaagagggc ggcgtggcgc ggatggcgtc
      901 gatcaaagtg atacgggaga ttatggcgac ggtgaccatt ccagtgatgg ccaaggtgcg
      961 cattggacac ttcgcggaag cggatgtttt gcaggcgctg gaagtggatt ttattgatga
     1021 gagcgaagtg ctgactccgg cggatgagga gaatcacatc tggaagcacg attaccgggt
     1081 gccttttgtg tgcggcgcga ggaatcttgg cgaagcgctg cggcgcattg cggaaggcgc
     1141 ggcgatgatt cgcaccaagg gtgaagcggg atccggcaat gtggtggaag cggtgcgaca
     1201 tatgcgcacg attgtttcgc agatgaagaa attgactacg ctgcgcgacg acgaattgat
     1261 gcacgaggcc aaggagctgg gagctccgat tgatttgatt cgacagattg cgaagacggg
     1321 aaagctgccg gtgccgaatt tttcggctgg cggtatcgct actcctgcgg acgcggcgct
     1381 ggttcggcga cttggggcgg aggcggtctt cgtgggttcg gggattttca agtccagcga
     1441 tccggaggcg cgcgcgaagg cggtggttaa agcgaccacg cactttgacg atccgaaggc
     1501 ggtacttgaa gcgcatgaag agctgggtga ggccatgaag ggtctggaca ttcgacagat
     1561 ggaagaaaaa gacatgatgt ccacgcgcgg ctggtgagcc tggtttcgac ggatggtaag
     1621 agatgaatca gcggagacga gtgagtgaag attgggattt t
//
LOCUS       DATCBB010000861         1153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_417018, whole genome shotgun sequence.
ACCESSION   DATCBB010000861 DATCBB010000000
VERSION     DATCBB010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417018"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            721..>1153
                     /locus_tag="VII37_09505"
     CDS             721..>1153
                     /locus_tag="VII37_09505"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070016.1"
                     /translation="MPIPRPRRKANTKQQIIAALKSAARKLGHTPTCVELYLLTGISR
                     NQVAWRFHGYRAAVRAAGLEPENNGVRIETAALLHDWGNVLRKVDAVPSGREYELAGR
                     YSRFAFYYRFKGWTAIPAAFCKSVAAGALTGDWTDVLEIIRR"
BASE COUNT          274 a          341 c          287 g          251 t
ORIGIN      
        1 gacggacgtg ataaaaaatg tgcggatttc ctgcggggcg tctttcattt tccaccagcg
       61 ctaaagcgca ttatcttaaa cgatccttac gcggccataa atggccgctc ttccaccgtc
      121 cggacaagat caaaagaaac tcgacgagaa tgctccgccg atccaaatac tttttcttcg
      181 cgctccagca cgaagaaccg gcaccgtcat ttttcttcgc gctcctgagc gcgaagaagt
      241 ggtggaagaa cggcccttta gggccgtgaa aaggaatgct tttagaacaa gggctttagc
      301 cctggtgccg caggacccaa ttccatcccc ggaaacgcgg aggaataggg tacgcagatg
      361 gcgtctgcca gcccaaaggg ccgctcttcc accgaaaacc atatgtcaag atatcctccc
      421 tcaaaacgag ccgcccacgc cccgcacata atcttccatt tgacaaaatc cgcttgttat
      481 cagacgctga tcgtcaacca cccgcctgct caaaaaatca tttcctaaat tatcgcgacc
      541 gcacaatacc acttgctttc gtgattcatt cgtgctataa actatccagc tcgcctcgcg
      601 cggcgggctg attttcagtc gccctgcaat gtgttgttcc caatcagcag catttcaatt
      661 cagggaagaa gtgtatcttt ttccaggtca ctcttctcct tttgcgaggg gggctaaacc
      721 gtgccaatac ccagacccag gcgaaaagcc aataccaaac aacagatcat cgcggcgctt
      781 aagtcggcag cgcgcaagct cggccatacg cctacctgcg tcgagctcta ccttttgacc
      841 ggcatcagtc gtaatcaggt agcgtggcgt ttccatggct atcgcgcagc agtacgcgcc
      901 gcgggactgg agccggagaa caacggagta agaatcgaaa ccgccgccct gctccacgat
      961 tggggaaacg tgcttcgcaa ggtggacgct gtgccctcag gcagggaata tgagctggct
     1021 ggccgttatt cgaggttcgc cttttactat cggttcaagg gatggacggc cataccggct
     1081 gcattctgca aatccgtcgc cgccggtgcg ctcactggcg attggaccga cgtgctggaa
     1141 atcatccggc gca
//
LOCUS       DATCBB010000862         1071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_383652, whole genome shotgun sequence.
ACCESSION   DATCBB010000862 DATCBB010000000
VERSION     DATCBB010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383652"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..767)
                     /locus_tag="VII37_09510"
     CDS             complement(318..767)
                     /locus_tag="VII37_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070017.1"
                     /translation="MVRALGALASLYTFCGLAGQSAYADGSVSSLRMGYGAPSEYAFA
                     QFLSVIQQYNASGERFRIDTHCQSACTMFLSIRNVCIAPGATLLFHAGGSMRKGIISP
                     YYTQQMLNAYNAALRRYVTDNHFMETFAFHPISGSEVIKRFGYPACR"
BASE COUNT          244 a          288 c          298 g          241 t
ORIGIN      
        1 cgcgcgcctt gtgcagggat tgtggcagga gtggactcgc caggtgagtc cgctcaggtc
       61 gatcaattgt tcacagcatg ggataaatct gcgtcgccgg gctgcgcttt ggcgatcacc
      121 gttgcttctg gtttcaaccg actctttagc ggcagcggca atcgaatccg gtttacacgc
      181 gatacacagg gcaacaggtg cactgctcaa tagctccaga gtgtacaaac tttcgatttg
      241 agcgttcgca atgaaaaatt gtggtttccg atcctcatcg cgcttctggt ggcttcgaaa
      301 ttccaggtca acctgcctta ccggcatgcg gggtagccga agcgcttgat gacttcgctc
      361 cctgaaatcg gatgaaaggc gaacgtttcc atgaaatggt tgtcggtgac ataccggcgc
      421 aaggcagcgt tataggcatt gagcatttgc tgcgtgtagt aggggctgat aatgcccttt
      481 cgcatagagc cgccagcgtg aaacaacagc gtcgcccctg gcgcgatgca gacattgcgg
      541 atcgaaagga acatcgtgca cgcggattga cagtgggtgt caatgcgaaa gcgctcgccc
      601 gacgcgttat actgctggat cacggatagg aattgcgcaa acgcgtattc actgggtgcg
      661 ccgtagccca ttcgcaagga tgacacggag ccatcggcgt aggccgactg ccccgcgagg
      721 ccgcagaaag tgtaaagaga tgcaagcgcg cccaatgcgc gcaccaattg ctgccctcta
      781 tgcaaaacgc ttctcctaaa tcggttggat ttttgagcgg cgcatagccc tgcgttaagc
      841 ttcgacctca atccagaacg agcaccggtt ctcaataaag tacgagcact gactgatcaa
      901 ccttgttagc tttcgcaatc cgatactcac ctctttccgt actccgtgaa aacgtagtcg
      961 cggttctgca ccaccgtgtg gcacgcgaat ccgctcgttc tggattgagg tcgaagctta
     1021 acgcagggct atgcgccgct caaaaatcca accgatttag gagaagcgtt t
//
LOCUS       DATCBB010000863         1678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_188910, whole genome shotgun sequence.
ACCESSION   DATCBB010000863 DATCBB010000000
VERSION     DATCBB010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188910"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VII37_09515"
     CDS             complement(<1..547)
                     /locus_tag="VII37_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070018.1"
                     /translation="MNAPANFPASPMAGKKMLPESEFIAGFHAIGEERYHHQHPFHLL
                     MHEGKLTRGQLQAWALNRYYYQSHIPMKDAAILAHSEDPAFRLAWRKRVIDHDGDGNN
                     PGGIEKWLRLVEATGLPRQQALHGDGILPATRFAVQAYVNFVSTHSLLESVASSLTEL
                     FSRKLITLRVDRLRQHYPWLAS"
     gene            complement(544..1545)
                     /locus_tag="VII37_09520"
     CDS             complement(544..1545)
                     /locus_tag="VII37_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5070019.1"
                     /translation="MRVKVLGSAAGGGFPQWNCACANCQRVRTGKFHGRARSQTQLAF
                     STNSKVWFLLSASPDLRTQLIATPELSPSRASGASARIGALAAALPAPVESPIGGVFL
                     PSADVDSVAGLLHLREFQSFFVFATPSVLSALQKENRVFRVLDRADPPVKWVRLSSKG
                     RLGCHLEEELGSAPEFLCTTVPLGGCYPDYVSEELRRTLIAEEATSAFVIHKNDKKIF
                     YAPAIPADTRPANGHAGAWSSWIENCDASFVDGTFWSDDELIKTGRSKKTARQIGHTP
                     LSGPSGMLEQYPKNIKGRKILIHINNTNPILDEDSPQHRAVLDAGFELAYDGMELEL"
BASE COUNT          367 a          444 c          488 g          379 t
ORIGIN      
        1 cactcgcgag ccacggataa tgctggcgca gccggtccac ccgcaaggta atcagcttgc
       61 gcgagaaaag ttcggtcagc gaagaggcca ccgattccag caacgaatga gtgctcacaa
      121 agtttacgta ggcctggacc gcaaaacgtg ttgccggcaa tatgccgtcg ccgtgcagcg
      181 cctgctgacg cggcaatccg gtggcctcca ccagtcgaag ccacttttca attcctcccg
      241 ggttatttcc gtcgccatcg tgatcgatca cgcgcttgcg ccatgccaga cggaacgctg
      301 gatcctcgct gtgcgccaga atggctgcat ccttcatcgg gatatggctt tggtagtagt
      361 agcggttgag ggcccaggct tgcagttgcc cgcgtgttaa cttgccttca tgcatcaaca
      421 aatgaaaagg atgctgatgg tgataacgct cttcgccaat ggcgtgaaat ccggcgatga
      481 attcactttc cggcagcatt ttttttcctg ccatggggct agcagggaag ttagcgggcg
      541 cgttcataac tctagttcca ttccatcgta ggccaattca aaaccggcat ccagcacggc
      601 gcgatgctgc gggctgtcct cgtcgaggat gggattggta ttgttgatat ggatgagaat
      661 tttgcgtccc ttgatgtttt tggggtactg ctcgagcatt ccgctaggac cggaaagggg
      721 agtgtgaccg atctggcgag cggttttctt gctacgcccg gtcttaataa gttcatcgtc
      781 gctccagaaa gttccgtcga caaaggaagc gtcgcagttt tcgatccagg aactccacgc
      841 accggcgtgt ccattagcgg gtcttgtgtc cgcgggaatt gcgggagcat aaaatatttt
      901 cttatcattt ttatgaatta cgaaggcgga ggtggcctcc tcggcgatga gtgtccgccg
      961 gagctcttcg cttacgtaat ctggataaca gccccccaag ggaacggtgg tacagagaaa
     1021 ctctggcgcg gagcccagct cttcctcgag atggcagccc aaacggcctt tgctggatag
     1081 ccgcacccat tttacgggcg gatccgcgcg atcgagaacg cgaaacacgc gattttcttt
     1141 ttgcagcgcg gacagcactg agggtgtggc gaaaacaaaa aatgattgga attcgcgcaa
     1201 atgaaggagt cccgctacgg agtctacgtc agcacttggt aaaaacacgc caccgatagg
     1261 ggactcgact ggcgcaggca gcgcggctgc gagcgcgcca atgcgagccg aagcgcctga
     1321 ggcacggctt ggagacaatt caggcgtggc aatgagttgc gttcgcaggt ctggagaagc
     1381 gctaagaaga aaccaaactt tggagttggt actaaaagct agctgggtct ggcttcgcgc
     1441 gcggccgtga aattttccgg ttcttaccct ttggcaattg gcacaagcgc aattccactg
     1501 gggaaaacca cccccggctg ctgagccgag gacttttacg cgcattcgat caggatccaa
     1561 gcagaccgcg aagcggtcat cgaactacag ctcagcgttg gcgtaggaac tgatttcgca
     1621 gttcaggtcg atctcttcgt gtgtcggtgt ggtccattcc atggtgaatc cccctggt
//
LOCUS       DATCBB010000864         3908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_417095, whole genome shotgun sequence.
ACCESSION   DATCBB010000864 DATCBB010000000
VERSION     DATCBB010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417095"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="murE"
                     /locus_tag="VII37_09525"
     CDS             <1..836
                     /gene="murE"
                     /locus_tag="VII37_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HEY5070020.1"
                     /translation="WGCHFSSAVFTNLTREHMDYHKTFEDYFAAKKTLFTGTGAGAPD
                     TAIINIDDDYGKKLLALANKAVSYGLESGADITTKKFQLSFSGVSFTAQTPNGKVQIT
                     SSLVGRINVYNILAAIGAAQALGFSNEVIEAGIRNLESVSGRFQRIDQGQPYLVIVDY
                     AHTDDALESLIRTARELNPKGRIITLFGCGGSKDRTKRPVMGEVTGRLSDLTILSSDN
                     PRQEDPLKIISDIIVGVQKTSGRYVIEPDREKARAALIQRGYKDPAATIGLHKNGSVV
                     K"
     gene            1043..2482
                     /gene="murF"
                     /locus_tag="VII37_09530"
     CDS             1043..2482
                     /gene="murF"
                     /locus_tag="VII37_09530"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY5070021.1"
                     /translation="MLWTIAQMAAAVGSKPGGALDPVARLAGVSIDSRTIRPGELFVA
                     IHGPRHDGHDHVPAALERGAVAAVVAEGLVSRYPGWVADRCIQVGDTFEALKQFAQAV
                     REAWGGKIAGITGSVGKTTTKEILAALLGAKLRVLKSEGNFNNEYGLPLTLFGLDESH
                     QAAVLEMGMSRPGELKRLAAIARPDVGVVTRVSPAHLEFFISVDEIALAKRELIEGLN
                     GKNSVAVLNADDPRVAAFASHAPGKVLTYGIDSPADFRAEQLENRGALGSAFTLVTKE
                     GRVRLEIALAGRHVIANALAALAAASVWGIGAEEAHGVLANLKAPFMRGEVVALSNGA
                     ALINDSYNSSPAALQAMTAVLAATPKYHRRILAAGEMRELGRTSAELHREAGRFAASA
                     EKIDWIIGVAGDAAQIIEGAVAAGISRAHTKFFSTSEEAAKFLAGFVESGDVMLVKGS
                     RGVKMERIVDELLTRYPAKAGASREEARH"
     gene            2483..3616
                     /gene="mraY"
                     /locus_tag="VII37_09535"
     CDS             2483..3616
                     /gene="mraY"
                     /locus_tag="VII37_09535"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY5070022.1"
                     /translation="MLYYLLYHTLQKYFSPLNVFRYITVRTVYASLTGMFLAFVFGPW
                     LIRQLRELQIGQYIREEGPQDHKKKAGTPTMGGVLIVLSTAVPTLLWADLGNAFVWLA
                     LFSLLGFAAIGFVDDYAKVSKQRNLGLTSAKKFSLQILVSLVCGIGLLVLATWSKYST
                     QLVFPFFKRVHPDLVIHSLMRSPHIWPLAFLPFLFFVSLVIAGASNAVNLTDGLDGLA
                     IGCTVIAAGALTVLTYVSSNFRWAAYLEIQYIPGVGELTVFCGALVGASLGFLWYNAH
                     PAEVFMGDVGSLSLGGALGLIAVIIKQEILLFFIGGIFVVEALSVILQVGSFKLRGKR
                     IFRMAPIHHHFELLGWSESKVIVRFWILALIFALFALTTLKLR"
     gene            3677..>3908
                     /locus_tag="VII37_09540"
     CDS             3677..>3908
                     /locus_tag="VII37_09540"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070023.1"
                     /translation="MTGGFELRGKHVLVIGLARTGIATARFCAEHGATVTATDTHSES
                     ELGAEALSLREEGVTLELGAHPETALPHQDLVI"
BASE COUNT          818 a         1008 c         1185 g          897 t
ORIGIN      
        1 tgtggggctg ccatttttca tccgcagttt tcaccaacct cacacgcgag cacatggatt
       61 accacaagac gtttgaagac tatttcgccg cgaagaaaac tctattcacg ggcaccggag
      121 cgggtgctcc tgacacggca atcattaaca tcgatgatga ctatggcaag aagcttctcg
      181 ccttggccaa caaggcagtc agctacggac tcgaaagcgg cgctgacatc accacaaaaa
      241 agttccaatt aagtttcagt ggggtttcct tcaccgcgca aacacccaac ggcaaagtcc
      301 agattacttc cagccttgtg gggcgcatca acgtgtacaa cattcttgcg gccatcggcg
      361 ccgcacaagc gctcggcttt tccaacgaag tgatcgaagc gggtattcgg aatcttgaaa
      421 gtgtgtccgg ccgattccag cgcatcgatc aaggtcagcc ttatctggtc atcgtcgatt
      481 acgcgcacac cgacgatgcg ctcgaaagcc tgattcgcac cgcgcgcgag ctaaacccca
      541 agggccgcat catcacgctg ttcggctgcg gcggctcgaa agaccgcacc aagcgtccgg
      601 tgatgggcga agttactggg cggctcagcg atctgaccat tctctccagt gataatccgc
      661 ggcaggaaga tccgctcaaa atcatcagcg acattattgt cggagtgcaa aaaaccagcg
      721 gcagatatgt gatcgagccg gaccgcgaaa aggcgcgagc cgcgttgatc cagcgaggtt
      781 ataaagatcc agcggcgacc atagggttgc ataagaatgg gagcgtggtt aagtaaaacg
      841 cagagtggtt ttacataaag tgcatcgata atttggcacg cggctcgctc taattattgg
      901 ggtgagccaa gcgcgcaggc actggaatag cgcgacacgc agcacccgat tttttgcact
      961 acctgaaaca atggggtgcc agaatgagaa gtacatcgca atcgcttgct tctgtacaca
     1021 aaccaaatcc gctgaggact ttatgctctg gacaattgca caaatggctg cagcggtggg
     1081 ttccaagcca ggtggggcgc tcgatcccgt ggcccggctg gccggcgtct cgattgattc
     1141 tcgcacgata cgtcccggag aactgttcgt cgccatacac ggaccgcgtc acgatggaca
     1201 cgatcacgtt cccgccgcac tggagcgcgg cgcggtggcg gcggtggtgg ccgaaggact
     1261 ggtatcgcgt taccccggct gggtcgctga ccggtgcatt caggtgggcg acaccttcga
     1321 ggcgcttaaa caattcgcgc aagcggtacg cgaagcatgg ggcgggaaaa ttgccggcat
     1381 cactggctcg gtgggcaaga ccacaaccaa agagattctc gcggcactgt tgggcgcgaa
     1441 attacgcgtg ctgaaatcgg aaggcaattt caataacgag tatggtttac cgctaacgtt
     1501 atttgggctg gacgaaagtc atcaggcggc ggtgctggaa atgggcatgt cgcggcccgg
     1561 tgaactgaaa aggttggcgg ccattgcacg tcccgacgtt ggagtggtga cgcgcgtttc
     1621 gccggcgcat ctggaatttt ttatttccgt ggatgagatc gcactcgcca aacgcgaatt
     1681 gattgaagga ttgaacggca agaactctgt ggcggtgttg aacgcggatg acccgcgtgt
     1741 ggcggctttc gcttcccacg cgccgggaaa agttttgacc tacgggatcg attcgccggc
     1801 cgattttcgc gcggaacaat tggagaatcg cggcgcttta ggatcggcat tcacgctggt
     1861 taccaaagaa ggacgcgtgc gcttggagat cgcgctggcg ggccggcacg tgatcgctaa
     1921 tgcgctggcc gcgttggcgg ctgccagtgt gtggggcatt ggagcggagg aagctcacgg
     1981 cgttctcgcc aatctcaagg cgccatttat gcgcggagaa gtggtggcgc ttagcaacgg
     2041 ggccgcgctg atcaatgaca gttacaactc cagccccgct gcgctgcagg ctatgactgc
     2101 tgttttggcg gcgactccca aatatcaccg ccgcattctc gccgcggggg aaatgcgcga
     2161 actgggacgc acttccgccg aactgcaccg cgaagcggga cgatttgcag ctagcgccga
     2221 aaaaatcgat tggattatcg gggttgctgg tgatgccgcg caaatcatcg aaggggcggt
     2281 ggccgcgggt atatcccgcg cgcacaccaa gttcttttcc acttcagaag aagcggcaaa
     2341 atttttggcg gggttcgtgg aatcgggcga cgtcatgctg gtgaaaggct ctcgcggcgt
     2401 gaagatggag cgcatcgttg acgagctgct cacgcgctat cccgcgaagg ccggcgcttc
     2461 acgggaagag gcgcggcact agatgcttta ctacctcctc taccacacgc tgcaaaaata
     2521 tttcagtccg ctgaatgtgt ttcgctacat caccgttcga acggtgtatg ccagcctgac
     2581 gggaatgttt ctggcgttcg tattcgggcc atggttgatt cggcagttgc gagaattaca
     2641 gattggccag tacattcgcg aagagggccc gcaggatcac aagaaaaaag caggtacgcc
     2701 gacgatggga ggcgttttga ttgtgttgtc gacggcggtg cccacgctgc tgtgggccga
     2761 tctgggcaac gctttcgtgt ggctggcgct tttctcgctg ttgggctttg cggccatcgg
     2821 atttgtcgac gattacgcga aagtttccaa gcagcgcaac ctcgggctga ccagcgcaaa
     2881 aaaattctcg ctgcaaattc tggttagttt ggtttgcggc attggactgt tggtgctggc
     2941 gacctggagc aaatactcca cgcaactggt ttttcctttt ttcaagcgcg tgcatcctga
     3001 tttggtcata cattccttga tgaggtcgcc gcacatttgg ccgctggcgt tcctgccgtt
     3061 tttgtttttt gtttcgttgg tgattgccgg agcatcgaac gccgtcaatc tgacggatgg
     3121 cctcgacggc ctggccattg ggtgcacagt gattgctgca ggggcgctga cagtgctcac
     3181 ttacgtgagc agcaattttc gttgggccgc ttatctagaa attcaatata tcccgggcgt
     3241 tggtgagctt accgtttttt gcggggcgct ggtgggtgca tcgcttggtt ttctctggta
     3301 caacgcgcat cccgcggaag tttttatggg cgatgtcggc tcgctttctt tgggcggagc
     3361 cctcgggctg atcgcggtga tcatcaagca ggaaattctg ttgtttttta ttggtgggat
     3421 ttttgtggtg gaggcgctct cagttatttt gcaggtgggg tctttcaagc tgcgtggtaa
     3481 acgtatcttt cgtatggcgc cgatacatca tcactttgaa cttttaggct ggagcgagtc
     3541 caaggtgatc gtgcgtttct ggattcttgc actgatcttc gcgttatttg cattgacgac
     3601 gcttaaattg aggtagcgcc gaatttttgg caatgccaaa gtgtgagcgt agtagggaaa
     3661 ggcttttagc acagcgatga caggcggatt cgagctgcgc ggcaagcacg ttctggtcat
     3721 tggactggcg cgcaccggta ttgctacggc gcggttttgc gcggagcacg gcgcgaccgt
     3781 gactgccacg gacacccact ctgaaagtga acttggcgcg gaggcgctca gcttgcgcga
     3841 agaaggcgtc acgctggaac ttggcgcgca tccggaaacc gctttgcctc accaggatct
     3901 ggtaatcc
//
LOCUS       DATCBB010000865         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_417126, whole genome shotgun sequence.
ACCESSION   DATCBB010000865 DATCBB010000000
VERSION     DATCBB010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417126"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VII37_09545"
     CDS             complement(<1..289)
                     /locus_tag="VII37_09545"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY5070024.1"
                     /translation="MLVVLKSNYEELSLEAARIVANSVRQNPSLRLGLATGSTTIGVY
                     RELVRLHREESLDFSRVITFNLDEYLGISPDHAQSFHHFMSEHFFSHVNLDA"
     gene            546..>2259
                     /locus_tag="VII37_09550"
     CDS             546..>2259
                     /locus_tag="VII37_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY5070025.1"
                     /translation="MALTTTDWIVIAAYLLLNLLISLYYRRRSSGSTEEFFVSGRNVS
                     WWLAGTSMVATTFAADTPLLVAGLVAKNGISGNWLWWSQCLCGMMTVFFFARYWRRAE
                     ILTDVEFVELRYEGKPAAFLRGFRAIYLGALMNCLILGWVIKAMISITTVLLGDAIAA
                     GRVLQIAVGGHVLLHYTLGAPAHTALLICVLILVPFTGIYTFIGGLWGVLVTDLFQFI
                     LKMTMIVVLAWIAVSKLGGMTMLKIQLSHVDSMVRQSGQTTGSILSFFPSFHLGWTTD
                     ALWTLPVITFCLYLAVQWWASWYPGAEPGGGGYVAQRMFSAKDEKNSLGATLWFNIAH
                     YAMRSWPWILTGLVAVAVYSPHGGLHPSMEFAAEPEKGYVMVLRDFLPPALRGLMVAA
                     FLAAFMSTVGTQLNWGTSYLINDLYRRFLVRKATDKHYVFVSKIIIVFLVVLSGYAAA
                     NINSIQSAWQLLLGMGAGTGSVLLLRWYWWRINAWSEISAMIAAFVVSISLQRVHFEG
                     NDSVVFAKAALITTITTTVVWLVTTMITKPESDERLVKFYRRVHPTVIGWQRIAALAP
                     EITPV"
BASE COUNT          449 a          697 c          568 g          545 t
ORIGIN      
        1 gggcatcaag attcacatgc gaaaaaaaat gctctgacat gaaatggtga aaactctgcg
       61 catggtcagg agaaatcccc aggtattcgt ccagattgaa cgtaatcacc cgggaaaaat
      121 cgagtgattc ttcgcggtgt aatcgcacca gctcgcggta cacgccaatc gtggtgcttc
      181 cggtcgccaa accaagccgc agcgaagggt tctggcgcac cgagttggcc acaatgcgcg
      241 ccgcctccag gctgagctct tcataattgg attttaggac caccagcatt taacgcacgc
      301 ccgcctgttt ttgtggaatc gactgatgcg ctcagccacc aaacttttct taccgtcacc
      361 cttgccattt aaacttttct aggccaccat tctgtgcttc gcagcgtccc gatagactac
      421 aacatccgcg ccatcaattg ctggccgggc cattactaaa actatttaac ccgtccacgc
      481 gaccggccag gggtagcctg aatttgcgct accgcgcgaa cgcagtaggg gagtagacac
      541 ccaagatggc gctcaccacc accgactgga ttgtaatcgc cgcctatctg ctgctcaatc
      601 tgttgatcag cctgtattac cgccgtcgtt catcaggcag caccgaagaa tttttcgtat
      661 cgggacgaaa cgtttcctgg tggctcgccg gcacatccat ggtggccacc acctttgccg
      721 ccgacacccc tctgctggta gccggcttgg tagccaaaaa cggaatctcc gggaactggc
      781 tgtggtggtc gcaatgtctc tgcggcatga tgaccgtctt tttcttcgcg cgttattggc
      841 gtcgtgcgga aattcttacc gacgttgaat tcgtagaact ccgctacgaa ggcaagcccg
      901 cagccttcct gcgcggtttt cgcgccattt atcttggcgc gctcatgaat tgcctgatcc
      961 ttggctgggt catcaaagcc atgatcagca tcaccaccgt tttgttaggc gacgccatcg
     1021 ccgccggccg cgtgttgcaa atcgccgtag gcggccatgt gctcctgcat tacacgctgg
     1081 gagcgcctgc ccatactgcg ttgctgatct gcgtcctcat cctcgtgccc tttaccggca
     1141 tttacacatt catcggcggc ctatggggcg tgctggtcac cgatctgttc caatttattt
     1201 tgaagatgac catgatcgtg gtcctcgcct ggatcgccgt ctctaagctc ggcggcatga
     1261 ccatgctcaa aatccaacta tcccacgtgg acagcatggt ccgtcaatcg ggacaaacca
     1321 ccggatcgat cctctccttt tttccttcct tccacctggg ctggaccacg gacgcgttgt
     1381 ggaccctccc cgtcattacc ttttgccttt atctagcggt ccagtggtgg gccagctggt
     1441 atccaggtgc ggaacccggc ggcggtggct acgttgcaca acgcatgttc agcgcgaaag
     1501 acgaaaaaaa ctcactcggc gcaacgctgt ggttcaacat cgcccactac gccatgcgtt
     1561 cctggccctg gattctaacc ggcttggtag ctgtcgccgt ttattcccct catggcggct
     1621 tgcatcccag tatggaattt gccgctgagc cggaaaaagg ctacgtgatg gtgctccgcg
     1681 attttcttcc ccccgcactc cgaggattga tggtcgcagc cttcctggcc gcgttcatgt
     1741 ccaccgtcgg cacccaattg aattggggaa cttcctacct catcaatgat ctgtaccgcc
     1801 gcttcctcgt gcgcaaagcc accgacaagc actacgtctt cgtcagcaaa attattatcg
     1861 tgttcttggt cgttctgagc ggctacgcgg ccgccaacat caattccatc cagtccgcgt
     1921 ggcaattgct gctgggcatg ggcgccggaa ctggcagcgt tttgctgctg cgttggtatt
     1981 ggtggcgcat caacgcctgg agcgaaatct ccgccatgat tgccgccttt gtggtttcca
     2041 tctccctgca acgcgtccat ttcgaaggca acgattcggt tgtcttcgcc aaagccgccc
     2101 tgatcaccac catcaccacc accgtggtgt ggttagtaac cactatgatc accaagccgg
     2161 aatccgacga acgtctcgtg aaattctatc gccgggttca tcctaccgtc atcggttggc
     2221 agagaattgc ggcactggct ccggagatca cccccgtgc
//
LOCUS       DATCBB010000866         1691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_223094, whole genome shotgun sequence.
ACCESSION   DATCBB010000866 DATCBB010000000
VERSION     DATCBB010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223094"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="nadC"
                     /locus_tag="VII37_09555"
     CDS             <1..907
                     /gene="nadC"
                     /locus_tag="VII37_09555"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY5070026.1"
                     /translation="RTPAPTRYFMDWNSRRITGIIENALLEDRATRDATSYACIEASQ
                     RASATILAKQDCILAGIGCVSRILDVYAVLDGAVLSHYEVTSHPEIFDGVRLHKGQAI
                     AVIRHNARVILSCERVILNFLQRLSGIATLTRKFVEAVSGTKARILDTRKTVPGFRVL
                     DKYGVRCGGGQNHRLDLSDGVLIKNNHIALAGGIVPALERANRNRRGTQPTEIEVRSL
                     EEMEQALQHGAEAILLDNMSVADVRLAVEHCSGLERRIPLESSGGITLDNVRAYAETG
                     VDFISVGALTHSALAVDMSLRVAPE"
     gene            1051..>1691
                     /locus_tag="VII37_09560"
     CDS             1051..>1691
                     /locus_tag="VII37_09560"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY5070027.1"
                     /translation="MKLLTSHATVVVSGTKIAEELRVGRSEVWRLVQQLREIGVDIAG
                     HPATGYLLRSVPDLLLPDMLLPLLRGTIFSSISHYYKIGSTNEIAMEAASKGAPEGSV
                     FLAEQQTAGQGRGAHQWHSAQSAGIYCSVVLRPVLPPSEILILSLAAGLAVYDAIREV
                     DSRVVPDLKWPNDLLIKEKKFCGILTEMNAEVTRVRYIVVGIGINANQTNFPP"
BASE COUNT          371 a          451 c          489 g          380 t
ORIGIN      
        1 gaggacacct gcccctacgc gatactttat ggattggaat agccgccgca ttaccgggat
       61 cattgagaac gcgctgctcg aagaccgggc tacgcgcgat gcgacgagtt acgcttgcat
      121 tgaggccagc caaagagcct cggcaactat ccttgcgaag caggattgca ttttggcggg
      181 catcggatgc gtgtcccgca ttctggatgt ttacgctgtc ctcgatggcg ccgtgctttc
      241 tcattatgaa gtgaccagcc accccgaaat ctttgatggc gtgcgattgc ataaaggcca
      301 ggcgattgcg gtcatccgtc acaacgcgcg tgtaatcctc tcctgcgagc gcgtgattct
      361 taattttttg cagcgactga gcgggattgc cacgctcacg cgcaagtttg tcgaggcagt
      421 atcaggaaca aaagctcgca tcctcgacac cagaaagacg gtgcccggat tccgggtgtt
      481 ggataaatat ggagtgaggt gtggaggcgg ccaaaaccac cgcctggacc tttccgatgg
      541 ggttctgata aagaacaatc acattgcact cgccggtgga attgtgcctg cactggagcg
      601 cgcgaatcgt aaccgccggg gcacgcagcc caccgagatt gaagtgcgct cattagagga
      661 aatggaacag gcgctccagc atggagccga agccatcctg ctggataata tgtcggtcgc
      721 agatgtgaga ctcgcggttg aacactgctc ggggctggag cggagaattc cgctcgagag
      781 ttccggaggc attacgctcg acaacgttcg ggcctacgcg gaaactgggg ttgattttat
      841 ttcggttggc gccctcacgc actccgcact ggcggtcgac atgagcctgc gcgtcgctcc
      901 ggaataatta ttttccattg aaagttcctc ataacatttg ttgtttcaat ttcattagct
      961 cataattcca gcattcatca agtaattcca agccgaagag cgaacacggg atctcccaca
     1021 ccgcagacgg atgcgcggct gggtcggctc gtgaagctgc tgacatcgca cgcaactgtc
     1081 gtggtcagcg gcaccaagat cgcagaagaa ctccgcgtcg ggcgttccga ggtgtggcga
     1141 ttggtgcagc agctacgcga gatcggcgtg gatatcgctg gccatccagc gaccgggtac
     1201 ctgctgagat cagttccgga tttactgctt ccagacatgc ttttgccctt gctgcgcggg
     1261 acaattttta gctcgatctc gcactattat aagatcgggt caacaaatga gattgcgatg
     1321 gaagcggcca gcaaaggcgc gcccgagggc agcgtgttcc tggccgagca gcaaaccgcg
     1381 ggccagggac gcggtgctca tcagtggcac tccgcgcaat ctgccggaat ttattgctct
     1441 gttgtattga ggccggtttt gccgccttca gaaatcctga ttctttcgct ggccgcgggg
     1501 ctcgctgttt acgacgccat tcgggaagtc gattcgcggg tagttcccga cttgaaatgg
     1561 ccgaacgacc tgctgatcaa ggaaaagaaa ttctgcggca ttctgaccga aatgaatgcc
     1621 gaagttactc gcgttcgcta catcgtggtg gggattggaa tcaatgcgaa ccaaacaaat
     1681 ttccctcctg a
//
LOCUS       DATCBB010000867         3440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_60119, whole genome shotgun sequence.
ACCESSION   DATCBB010000867 DATCBB010000000
VERSION     DATCBB010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60119"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..>3440
                     /locus_tag="VII37_09565"
     CDS             496..>3440
                     /locus_tag="VII37_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY5070028.1"
                     /translation="MSSAAAQVNTATLTGTIRDPQGLGVKGGTIMLENSADGAQRTAE
                     ADESGRYNIVGLPPGSYHVTVEGGAGFGRYENPSLILTVGENATLDPQLSLGGVSQTV
                     TVSSETADIEPTKTEVSQTIEQRRINNLPINGRNYINFTLTNSQATRDVSPTIGPAPN
                     SGLNIDGARARSNMVSVDGADAVDNSVNGIRSTVSQEAVQEFQLILSNYNAEYGRATG
                     GVINIVTKSGGNDIHGDAFGFFRNKAFQARNAFSGEINPTTGALDPVKQAYTRVQAGA
                     TIGGALKKDKTFYFFSYEDTLREETGFSSIGIDNFGLQPVTLPTLSGPLTVQLTGPQA
                     TAVSSLLATGDPTLQGLAVQYGVLYGSASSVALNRLDFGAVSAGLTGGFLNPGPGAQF
                     PLPVSCPAGQTVNAVNCSAIGEGIAPLPSSFVGLNSTRGNFPVTEKTSLWSGRLDQRW
                     TDHNTSFIRVGVSPSLVTGVQSTSQNQVFGQNAGTRAGVNQYRDFSIVGQHDTILGDS
                     AFNEFRFQFARRGLHFGYSDLPGGSDIGVNIPGYAYFGREPYSTVDRLERRTEFTDHV
                     TLIRGNHTLKLGGDVNLIQLRSGKAQIFQLDFGGDVNFGGLAADTFGFPDSIPLGNGQ
                     SIALPGTTGLQSYGLGLPTTYIQGIGTSNAPFDNLPFAFFIQDSWRVNRHLAINYGLR
                     YDYEKTPLFAPATAINAAAENALGVLEGIPRDNNNFAPRLGIAWDPRGDGKMVVRAGY
                     GLFYDHPLLAIAFNSVTAEGGRSVQLLSAGGTPSACGLITPTCGNGLDTPNNLNGSSI
                     FQGVLNALPSMGYLPNQQRFDPLLPNSLFANQNYLTAGFPLPILPFTLPVAKNFKFAY
                     AEQGNLTIEREIYGTWKISVGYQYTRGLHLNRPVDINSTDPQLLANNLEFADAAGLGF
                     TNPLTVVAPGASFAAGTNGPNTCGVTVIAPQALGFLTGCPVAALNGQFVGTPAFFNFF
                     RPSGPNPSFA"
BASE COUNT          729 a         1112 c          816 g          783 t
ORIGIN      
        1 gtggagaaac gctggtatga atatttcctc cacaatcagc gcggccgtat gctggagtta
       61 ttacttctcg ttcacggcaa taaacgcgtg ttctcccttt ccgccccgta ttacctctcc
      121 gaagagatca gtaagtcctg agcagataag acttatcctt ttttttcccg aaagaacgtt
      181 ctgcgtagct cctgccgagt ccatctggga atctatggga ctactcttgt atctcatttg
      241 gcgacctatg caggagtcca tgcttgacac tgcttgacag cgggcatatg gttttccgcg
      301 aagacacacc agtcgcaaat ccgcgtcgca gccatattca aagacggccg ctgtgcgttt
      361 ttgtgccgct tcttcaactc tcgccgttga tccgttaggg gtagcggaac tcggtgatca
      421 atccttgggg gactcatgca ccgcagaatt cttatgcgca tgtgcttcgt gctatgcgct
      481 gtaatgcttt gcctcgtgag ttccgccgcg gcgcaggtta ataccgccac tcttaccggc
      541 accatccgcg atccgcaagg gctgggagtg aaaggcggga ctattatgct cgaaaattcc
      601 gcggacggcg cgcaacgcac tgctgaagcg gacgagtccg gccgttacaa catcgtggga
      661 ttaccgccag gaagttacca tgtcaccgtg gaaggcggcg cgggctttgg cagatacgaa
      721 aatccttctt tgatattgac ggtcggcgaa aacgccacac tcgatccgca gcttagtctg
      781 ggaggcgtat ctcaaacagt tacagtgtcc tccgaaaccg ccgacatcga gcccaccaaa
      841 acagaagtct cgcagaccat cgagcagcgc cgcatcaaca atttgcccat caatggccgc
      901 aactacataa atttcaccct gaccaattcc caggccaccc gcgatgtttc tcccaccatc
      961 ggccccgctc ccaacagcgg cctcaacatt gacggcgccc gcgcgcggtc caacatggtc
     1021 agcgtggatg gtgccgatgc tgtcgacaat tccgtcaacg gcatccgttc caccgtatcc
     1081 caggaagccg tccaggaatt tcaattgatc ctcagtaatt acaacgccga gtacggccgc
     1141 gccaccggcg gtgtcatcaa tatcgtcacc aagagcggcg gcaacgatat tcacggagac
     1201 gctttcggct ttttccgcaa caaagccttc caggctcgca acgcattttc cggcgaaatc
     1261 aatcccacta ccggcgctct agatcccgta aagcaggctt acactcgtgt gcaagcaggc
     1321 gcgaccatcg gcggggcgct caaaaaagat aaaacctttt attttttctc ctacgaagac
     1381 acgcttcgtg aagaaaccgg attttccagc atcggcatcg acaactttgg cttacaaccc
     1441 gtaaccctgc ccactctcag cggtccgctg acagtgcaat taacaggtcc gcaagccacc
     1501 gcggtaagtt cattgctggc gaccggcgac cccacgctcc agggattggc ggtgcaatac
     1561 ggcgttctct acggctcagc atcaagtgtc gctcttaatc gcttggattt cggtgcagtc
     1621 tcagccggac tcaccggcgg atttttaaat cccggcccgg gcgcgcaatt tccgttgccc
     1681 gtgagctgcc ccgccggtca aaccgtgaac gccgtcaact gctcagccat cggtgaaggc
     1741 attgcgccac tcccctcttc cttcgtgggc ctcaactcca cgcgtggtaa tttccctgtc
     1801 accgaaaaaa ctagcctgtg gtccggacgc ctcgaccagc gctggacgga tcacaacact
     1861 tcgttcattc gcgttggcgt ttcaccatct ctggttaccg gcgtgcagtc cacctcgcaa
     1921 aatcaagtct tcggccaaaa cgcaggaacc cgcgctggcg tcaaccagta tcgcgatttc
     1981 tccatcgtcg gacaacacga caccatcctc ggcgacagcg ccttcaacga atttcgcttc
     2041 cagtttgcgc gtcgcggcct gcatttcggt tattcggact tgcccggagg ctccgacatc
     2101 ggtgtcaata tcccgggcta cgcgtatttc ggccgcgaac cttattccac cgtcgatcga
     2161 ttggaacgcc gcaccgaatt cactgatcac gtcaccctga ttcgcggcaa ccacactctc
     2221 aaactgggcg gtgacgttaa cctcattcag ttaagatccg gcaaagctca aatattccaa
     2281 cttgattttg gcggcgacgt aaacttcggc ggcctcgctg ccgacacctt tggatttccc
     2341 gactcaatcc cgctcggtaa cggtcaatcc atcgcccttc caggcaccac cggcttgcaa
     2401 tcctacggct tgggcttacc caccacctac attcagggca tcggcacctc caacgctccc
     2461 tttgacaatt tacctttcgc ctttttcatc caggacagtt ggcgggtcaa cagacatttg
     2521 gccatcaatt acggcctgcg ctacgactac gaaaagactc cgttgttcgc cccggccacc
     2581 gccatcaacg ctgccgccga aaacgctctt ggcgtactcg aaggaattcc gcgcgataac
     2641 aacaatttcg ctccgcgcct cggcattgcc tgggatcctc gcggcgacgg caaaatggtg
     2701 gttcgcgccg gttacggact tttctacgat catccgctgc tcgcgatcgc tttcaactcc
     2761 gtcaccgcgg aaggcggccg ctccgttcaa ttattgtccg ccggcggaac tccctcggca
     2821 tgcggactca tcactcccac gtgcggcaac ggcctcgaca ctcccaacaa tctgaatggt
     2881 tcttccattt ttcagggcgt cttgaacgcg ctccccagca tgggttattt acccaaccag
     2941 cagcgcttcg atcccctgct gcccaattcg ctcttcgcca atcagaatta tctgaccgcg
     3001 gggtttccgc ttcccatctt gccgttcact cttcctgtag cgaaaaattt caaattcgcc
     3061 tacgccgagc aaggcaacct tacgattgag cgcgaaatct acggcacgtg gaaaatcagt
     3121 gtcggctatc aatacactcg cggcttgcat ttgaatcgcc cggtggacat aaattccacc
     3181 gatccacaat tgctcgccaa caatctggaa ttcgcagacg ccgccggttt gggcttcaca
     3241 aatcccctca ccgtcgtcgc ccctggagcg agttttgctg ccggaacaaa tggacccaac
     3301 acctgcggcg tgactgtcat tgccccgcaa gcactcggct tcttaaccgg ctgcccggtc
     3361 gccgcactca acggccaatt tgttggcact ccggcatttt ttaatttctt ccgcccgtca
     3421 ggacccaacc cttccttcgc
//
LOCUS       DATCBB010000868         1494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_92286, whole genome shotgun sequence.
ACCESSION   DATCBB010000868 DATCBB010000000
VERSION     DATCBB010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92286"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..>1494
                     /locus_tag="VII37_09570"
     CDS             81..>1494
                     /locus_tag="VII37_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070029.1"
                     /translation="MTANARRPMLHNEGLLTRNYTSYNFQVLRDDIYMLGVDGTVTGH
                     RIAPARSSCAVLVSSQNANREWLYYTQQTISAEGFSGQAYSTFVPHTVRGKETKQCSD
                     CHVSAANDNNAWMAQLLLQGTNFMNFMGRYVYVATGKGGMEAIAVAEHDEPEAIYGSD
                     LQRIAYPENYKKFVARHRELSAAAEHGGNILDIQARGEYAYTANGAGGLRVYDIANID
                     NKGFSERMVTAPVSPIGQRFYVPTKNAVAVASPSTLAIDPLRQRLPENEEQPIHLLYG
                     FLYVADKEEGLVIVGNPDLHAKSPGVGTLLDGNPANNFLKRAAAFNPGGVLSGARRIT
                     IAGTYAYIVTEKNLVVVDLNDPLAPRVTATIGAPILDGPAAIAIQFRYAFVVDRQGLK
                     VLDVTDLAQPKMVANALVALSDARNVYVARTYAYVSAGKEGMAIVDVEKPEAPKLDQM
                     FNAGGQLNDVNDVKIGMVAAS"
BASE COUNT          339 a          371 c          456 g          328 t
ORIGIN      
        1 tggctcacgg gaacagcagc atgacttgtt acgcgtgcca tacatcgtgg acgcccacct
       61 gtttcgggtg ccacctgcaa atgaccgcca atgcgcgccg gcccatgctg cacaacgaag
      121 gattgctgac gcgaaattac acctcctaca actttcaggt gctgcgcgac gacatttata
      181 tgctgggtgt ggatgggacg gtcaccgggc atcgcatcgc gccggcgcgt tcgagttgcg
      241 cggtgctggt aagttcgcag aacgcaaatc gggagtggct ctattacacg cagcagacga
      301 tttctgcaga gggattttct ggccaggctt acagcacgtt tgttccgcac acggtgcgcg
      361 ggaaggaaac caagcaatgc agcgactgcc acgtttccgc agcgaacgac aacaatgcgt
      421 ggatggcgca gcttttgttg cagggcacca acttcatgaa tttcatgggc cgttatgttt
      481 atgtggcgac tggaaaaggc ggcatggaag ctattgctgt cgcagagcat gacgaaccgg
      541 aagctatcta cggcagcgat ttgcaacgga ttgcttatcc ggaaaattat aagaaatttg
      601 ttgcgcggca tcgcgaactc tcagcggcgg cggaacacgg cggaaacatt ttggatattc
      661 aggcgcgagg ggaatacgcg tataccgcaa atggagcggg cgggttgcgc gtctacgaca
      721 tcgcgaatat cgataacaag ggtttttccg aacggatggt gactgccccg gtgtcaccca
      781 tcggccagcg tttctatgtg cctacgaaaa acgcggtggc ggtggcgtcg ccatccacgc
      841 tggcgatcga cccgctgcgg caacggctgc cggaaaatga ggagcagcct attcatttgt
      901 tgtatggatt tctttacgtt gccgataaag aagaaggttt ggtcatcgtc ggcaaccccg
      961 atctgcatgc gaagagtccg ggagtcggga cgcttctgga tggcaaccct gcgaataact
     1021 ttctcaagcg cgcggcggcg ttcaatccag gaggtgttct tagtggagct cgacgcatca
     1081 ctatcgcggg cacgtacgct tatatcgtaa cggaaaaaaa tttggtggtt gtggatctaa
     1141 atgatccatt ggcgccacgg gttacagcga ctatcggagc gccgattctt gacgggcccg
     1201 cagcgattgc cattcagttt cgttatgcgt tcgtggtgga tcgccaagga ctgaaagttt
     1261 tggatgtcac ggacttggcg caaccgaaga tggttgcaaa tgcgctcgta gcgttgagcg
     1321 acgcgcgcaa tgtttacgtg gctcgcactt atgcttatgt ttcagccggc aaagagggaa
     1381 tggcgattgt ggacgtggaa aagccggagg ccccgaagct cgatcaaatg tttaacgccg
     1441 gcgggcagct caatgacgtc aacgacgtga aaatcggaat ggtggctgcc agcg
//
LOCUS       DATCBB010000869         1129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_319135, whole genome shotgun sequence.
ACCESSION   DATCBB010000869 DATCBB010000000
VERSION     DATCBB010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319135"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VII37_09575"
     CDS             <1..1113
                     /locus_tag="VII37_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070030.1"
                     /translation="LLNFTWWVNRKDPDGSNIFQGGFLGLDNIGVFDRSAPLPTGGHL
                     EQSDGTSWMGMYCLNMLAIALELAKEDRAYEDIASKFFEHFVYIAHAMNNFGSQERSL
                     WDEEDGFYYDVLRLPNGDDQFLKIRSMVGLIPLFAVETLEPEAIDRLPGFRRRMQWFI
                     DNHPDVPDHIDMSQSSARGIRFLLSLVSKKQLGRVLSRMLDESEFLSPHGVRALSRFH
                     KDHPFELRVDGTVNTVNYEPAESTTGIFGGNSNWRGPVWFPVNYLLVESLQKYHHYYG
                     EDFKVECPTRSNKQMDLWEVAAEISRRLTHIFLRDDDGRRPVYGDTDVFQNDPHWRDL
                     ILFYEYFNGENGAGLGADHQTGWTGLVAKLIEQSGE"
BASE COUNT          252 a          269 c          352 g          256 t
ORIGIN      
        1 ctgctgaatt ttacctggtg ggtaaatcga aaagatccgg atggttcaaa tatttttcag
       61 ggcggatttc tggggctaga caatattgga gtattcgacc ggtccgcgcc gttgccgact
      121 ggcgggcacc tggaacagtc cgacggaacc agctggatgg gcatgtattg cctgaacatg
      181 ctggccatcg cgctggaact ggccaaggaa gatcgcgcgt acgaagatat cgccagcaaa
      241 tttttcgaac actttgtcta cattgcgcac gccatgaata atttcgggtc gcaggagcgt
      301 tcgttgtggg atgaagaaga cggtttttac tacgatgtgt tgcggctgcc caacggagac
      361 gatcagtttc taaaaattcg ctcgatggtt gggttgattc cgttgttcgc ggtggaaacg
      421 ctcgaaccgg aagctatcga tcgcctgccg ggtttcagac ggcggatgca gtggtttatc
      481 gacaatcatc cggatgtacc cgatcacatc gacatgtcgc agagctcggc gcggggaatc
      541 cggttcctgc tatcgctggt gagcaagaag cagctggggc gggtattgtc gcgcatgctg
      601 gacgaatcgg aatttctgtc tccgcacggg gtgcgggcct tgtcgcgttt ccacaaagac
      661 catccgttcg aactgcgcgt cgacggcacg gtgaacaccg taaattacga gccggcggaa
      721 tcgacgacgg ggatttttgg agggaattcc aactggcgcg gaccggtatg gtttccagtg
      781 aattatctgc tggtggaatc gctgcagaaa taccatcact attatggcga agacttcaag
      841 gtggaatgcc ccacgcgttc gaataagcag atggacctgt gggaagtagc ggcggaaata
      901 tcgcgacgtc tgacgcatat tttcctgcgc gacgacgatg gacgccgccc ggtctacggc
      961 gacacggatg tttttcagaa cgatccgcac tggcgggatc tgatcctttt ctatgaatat
     1021 ttcaatggcg agaacggcgc gggactggga gccgaccacc agacgggttg gacggggctg
     1081 gtggccaagc taatcgagca gagcggggaa tgagcagccg tggctaatt
//
LOCUS       DATCBB010000870         1268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_351946, whole genome shotgun sequence.
ACCESSION   DATCBB010000870 DATCBB010000000
VERSION     DATCBB010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351946"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /gene="nrfD"
                     /locus_tag="VII37_09580"
     CDS             complement(<1..821)
                     /gene="nrfD"
                     /locus_tag="VII37_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY5070031.1"
                     /translation="MSVAQPPIDSPEIPVLGPGHTYESVTEQISSAVLTRPTTLGWFA
                     GFTVAFSVLGMLIVAVGWLIIKGVGIWAINIPIGWGFAIVNFVWWIGIGHAGTLISAI
                     LYLLNQQWRTSINRFAEAMTLFAVACAGLFPLIHTGRPWMAYYMFPYPSTMGVWPQFR
                     SPLIWDVFAVSTYGTVSLLFWFVGLIPDLATLRDRAVEGWKQKTFGLLAMGWRGSARH
                     WHRYQSAYLLLAGLATPLVLSVHTVVSFDFSIAIVPGWHTTIFPPYFVAGAIYSG"
     gene            complement(818..>1268)
                     /locus_tag="VII37_09585"
     CDS             complement(818..>1268)
                     /locus_tag="VII37_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5070032.1"
                     /translation="MVYNRCIGTRYCSNNCPYKVRRFNFLRFQDWETPQLKLLRNPEV
                     TVRSRGVMEKCTYCVQRINNVRIDAEKENRPIKDGEIVTACQAACPSEAIIFGNANDS
                     STRVAKLKAQQRNYSILGELNARPRTTYLAAVRNPNPDLSTSTTEKE"
BASE COUNT          309 a          337 c          382 g          240 t
ORIGIN      
        1 aagccggaat aaatcgcgcc ggccacgaag tacggcggga agatcgtggt gtgccagccc
       61 ggaacgatgg caatcgagaa atcaaagctc actaccgtgt ggacggacaa gaccagcggc
      121 gtggccagcc cggcaagcaa caagtaggcc gactgataac ggtgccagtg tcgcgcggaa
      181 ccgcgccagc ccatggccag caatccgaag gttttctgtt tccaaccctc gacggcgcga
      241 tcacgtagcg tagccaaatc tgggatcaat ccgacgaacc agaacagcag cgaaaccgtg
      301 ccataggtag aaaccgcgaa cacgtcccag atcagcgggc tgcggaattg cggccagact
      361 cccatggtgc tgggatacgg gaacatgtaa taggccatcc acggacggcc agtgtgaatc
      421 agcggaaaca gacccgcgca agctacggcg aatagcgtca tggcttccgc gaaacggttg
      481 atggaggtgc gccactgttg attgagcaaa taaagaatgg cggaaatcag cgtgccggca
      541 tggccgatac caatccacca cacgaaattg acgatggcga atccccaacc gatgggaatg
      601 ttgatggccc agattccgac gcccttaatg atcagccagc cgacggcgac gatcagcatg
      661 ccaagcacgg aaaatgcgac tgtgaagccc gcaaaccaac ccaaagttgt gggacgagtc
      721 aaaacagcag aactgatctg ctcggttacg gactcatagg tatgaccggg tccgaggacc
      781 ggaatctccg gagaatcgat gggcggttgc gcgacgctca ttctttctcc gttgtggatg
      841 tcgagagatc gggattggga ttacgcacgg cagcaagata agtggtgcgc ggacgcgcat
      901 tcagctcgcc cagtatcgaa taattgcgtt gctgggcttt taacttggcg acgcgtgttg
      961 aagaatcgtt ggcgtttccg aaaatgatgg cttcgctggg gcacgcggcc tggcaggcgg
     1021 ttacgatttc tccgtctttg atggggcgat tttctttctc ggcatcaatg cgcacattgt
     1081 tgatgcgctg cacgcagtaa gtacactttt ccatgactcc gcggctgcgc acggtgactt
     1141 ccggattgcg cagcagcttc agttgaggcg tttcccaatc ctggaagcgg aggaaattaa
     1201 agcgccgcac cttgtagggg cagttgttcg aacagtagcg cgtgccgatg cagcggttgt
     1261 agaccatc
//
LOCUS       DATCBB010000871         1228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_60282, whole genome shotgun sequence.
ACCESSION   DATCBB010000871 DATCBB010000000
VERSION     DATCBB010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60282"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VII37_09590"
     CDS             <1..331
                     /locus_tag="VII37_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070033.1"
                     /translation="LEELAVAARQGKIESCLIRLENLLPNFPRMNVLPVIERRVRHGS
                     KFNISVAQIQPGRVEPPPGATAQLDGGDPRPPRLRVFSQQDKLIAIAEAVVPRTYQPV
                     VVFDPLP"
     gene            complement(427..>1228)
                     /locus_tag="VII37_09595"
     CDS             complement(427..>1228)
                     /locus_tag="VII37_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEY5070034.1"
                     /translation="TIDGTFTPGPSSLLATATTPAGVQVGSFSGGASPDIVVTNQGQS
                     TLSFFLGLGAGTFQTGVELNVPATPGALIVSQLTSSGLPDVALVAQGSTAGQGVVSII
                     QDSSALAALGSGTGQTPYPGAEYMDIGLKVKATPALHPNNEVTLQLEFEIRALAGGNI
                     NGIPIISNRTLTQTVRVKDDQPTLIGGLTDREETRSITGLPGFAELPGVGYAFGGRTN
                     SLSDTELMILITPHRLRSPQHQTGTIFAGHGERGVTPSTLPSERVQPP"
BASE COUNT          268 a          403 c          297 g          260 t
ORIGIN      
        1 actcgaagag ttagccgtgg cagcgcgcca aggcaaaatc gaatcctgcc tgatacgcct
       61 ggaaaattta ctgcccaatt ttccgcgcat gaatgtctta ccggtcatcg aacgccgcgt
      121 tcgccacggc tcaaaattta atatctccgt cgcgcaaatc caaccaggcc gtgtcgagcc
      181 gcctccaggc gccaccgcgc aactcgatgg cggcgaccct cgcccgccgc gtctgcgcgt
      241 cttcagccag caagacaaac tcatcgccat cgccgaagcc gtcgtgcccc gcacctacca
      301 gcccgtcgta gtcttcgacc ctttgcctta aatcccgcga tttttttctg aagatattct
      361 ggctctgtgg tggcgaatat cagaacctat aaaaattcct gcactgccag ttctactcta
      421 tccacatcac ggcggctgaa cccgctcgga aggcaacgta ctcggcgtaa ctccccgttc
      481 gccatggccc gcaaaaatcg tcccagtctg gtgttgtggc gaacgcaacc ggtgtggcgt
      541 aatcaaaatc atcagctcag tatcggacaa actgttggtt cgcccgccaa acgcataccc
      601 cactcccgga agctccgcaa atccaggcaa tccggtgatc gagcgcgtct cttcgcggtc
      661 cgtcagtccg ccgatcaaag tcggctgatc atctttcacg cgcaccgttt gcgtcagcgt
      721 gcgattcgaa atgatgggga tgccatttat atttccgccc gccaacgcgc gaatttcaaa
      781 ttccagttgc agcgtcacct cgttattcgg atgcaaagcc ggcgtggcct tcaccttcaa
      841 accgatatcc atatattcgg cgcccggata tggcgtttgc cccgtgccgc tgcccagcgc
      901 cgccagcgcg gaagaatctt gaataatgct aaccactcct tgtccagcgg tcgaaccttg
      961 cgccaccagc gccacatccg gaaggccgct gctggtgagc tgcgacacga tcagcgcccc
     1021 aggagtcgcg ggaacattca attcaactcc ggtctgaaat gttcctgcgc caagtcccaa
     1081 aaaaaagctc aacgtgcttt gtccttgatt agtcaccaca atatcgggtg aggcgccacc
     1141 cgaaaaactt ccgacctgta cgcccgccgg tgttgtagcc gtcgccaaca gagaacttgg
     1201 cccgggcgta aacgttccgt cgatggtc
//
LOCUS       DATCBB010000872         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_253889, whole genome shotgun sequence.
ACCESSION   DATCBB010000872 DATCBB010000000
VERSION     DATCBB010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253889"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VII37_09600"
     CDS             <1..323
                     /locus_tag="VII37_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070035.1"
                     /translation="PGDARELTPILRKSFSSVLTLLRHVIIAFGEEPPAEPREIIKRA
                     TALTHSDASAFETILRLREHTDAHMEIAQAYGAYLAAVESVIRALDLHLPKEQWQRTR
                     QAHS"
     gene            367..1008
                     /locus_tag="VII37_09605"
     CDS             367..1008
                     /locus_tag="VII37_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5070036.1"
                     /translation="MKIGIVILVILLILALVLGSAFVSRRNQMAIKREAVNAAWSQVD
                     VVLQRRADLIPNLVETVKGYAVQEQVVYGEIARARSALLNASTPADKIAANGQLDSAL
                     GRLLVIVENYPQLKSNENFMRLQDELAGTENRIAIERRNYNQVVQDYNTYISLFPNSL
                     VASMAGFTRNDAYFKTEPGARQAPKVNFDYPKNPGAETTPPAKPAPTPAPAHP"
     gene            1200..>2144
                     /locus_tag="VII37_09610"
     CDS             1200..>2144
                     /locus_tag="VII37_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY5070037.1"
                     /translation="MAATGAGASASDAPQIPRIIIHDNTEASGKLEDGVLTLRLEIRE
                     GEWHPDAEDGPGMPVLAFAEEGKSPSIPGPMIRVPQGTRIKVSVKNTLIFFPAFLHGL
                     NQRPGKDEAIKIAPGATQEVTFLAGEPGTYYYWANTLADIAVDSRQGWDTQLDGAFIV
                     DAAGTRNDDRVMVIGLWYAWVVPFDFSRGYHQFLTMNGKSWPHTTRLSYEMGETIHWR
                     VINASDGVHPMHLHGAYFQVDSTGDAQVEQRYAEGERRSVVTELMAEGSTMGVSWKPS
                     HAGNWIFHCHLADHFSEDNARQVSEVMGLPVEKMDHHAG"
BASE COUNT          469 a          552 c          657 g          466 t
ORIGIN      
        1 ctccaggcga cgcccgcgaa cttacaccta ttctgcgcaa gtctttttcg tcggtcctca
       61 ccttgctgcg ccatgtcatc atcgccttcg gcgaggagcc accagcggag ccgcgcgaaa
      121 tcattaagcg cgccacggcg ctgacgcact cagacgcttc cgcatttgaa actattctgc
      181 gtttgcgaga gcataccgac gcgcacatgg aaatcgcaca ggcctacgga gcatatcttg
      241 ccgctgtgga atcagtgatt cgcgcgctgg acctccattt gccaaaggaa cagtggcaac
      301 ggaccaggca ggcacattct tgaatttcag gcacccattc ttgaatttca cgtaattgag
      361 gagcatatga aaataggaat cgtcattttg gttattcttc tgattcttgc tttagtgctg
      421 ggcagtgctt tcgtaagccg gcgcaaccag atggccatca agcgcgaagc agttaacgcc
      481 gcttggtcgc aggtggacgt agtcctgcaa cgccgagccg atttgattcc taatttggtg
      541 gaaactgtca aaggttacgc cgtccaagag caggtcgtct atggcgagat cgcgcgagcc
      601 cgctctgctt tgctaaacgc cagcacgccg gccgacaaaa tcgccgcgaa cggacagttg
      661 gactccgcgc tcggccgttt gctggtcatc gtcgagaatt atccgcagtt gaaatccaat
      721 gaaaatttca tgcgcttgca ggatgaactg gcgggcaccg aaaatcgcat cgccatcgag
      781 cgccgcaact acaatcaagt cgtgcaggac tacaacacct acatctcgct attccccaac
      841 agcctggtcg ccagcatggc cggctttacc cgtaacgacg catacttcaa aaccgaaccc
      901 ggcgcgcgtc aggctcccaa ggtcaatttc gattatccga aaaatccagg cgcggaaact
      961 acgccgcccg ctaagccggc acccacgcct gcgcccgctc atccgtagga cagcgggagc
     1021 atagcggcac tatctgactc aaagacaaca ccgtgcgttc acggtacgaa aaagaaatga
     1081 ggcctcggac gcctcgtttt tgatgggttt tcctccggat agttatagaa agaggtttca
     1141 tgagtagcag gcaggcgatc tcgcgccctc ggcgaaggtg tttggttttt gtgtggctga
     1201 tggcggcgac tggggcaggg gcatcggcca gcgatgcgcc gcagataccg cgcattatta
     1261 ttcacgacaa tacggaggct tcagggaagt tggaggatgg ggttttgaca ttgcggctgg
     1321 agattcgtga gggcgagtgg catccggacg ctgaagatgg gccggggatg cctgttcttg
     1381 cgtttgctga ggaaggcaaa agccccagca ttcccgggcc gatgattcgt gtgccgcagg
     1441 ggacgcgcat caaggtgtcg gtgaagaata cgctgatatt tttcccggcg tttttgcatg
     1501 gcttgaatca gcggccggga aaggatgagg cgatcaagat tgcgccgggt gctacgcagg
     1561 aggtgacgtt tctggcaggt gagccgggta cgtattacta ctgggcgaac accctggcgg
     1621 atatcgcggt tgattcgcga caaggctggg acacacagct cgacggggca tttattgtgg
     1681 acgctgcggg gactcgcaat gatgaccgtg taatggtgat cggactttgg tacgcgtggg
     1741 tggtgccgtt tgatttcagc cggggatatc accagttcct taccatgaat ggtaagtcat
     1801 ggccgcatac gacgcggttg tcgtatgaaa tgggggagac cattcactgg cgggtgatca
     1861 atgccagcga cggagttcat ccgatgcatt tgcacggtgc ttattttcag gtggatagca
     1921 ccggggatgc gcaggtagag cagcgttatg cggagggcga acggcggagc gtggtgaccg
     1981 agcttatggc ggaagggagc acgatgggtg tgtcctggaa gccgtcgcat gcgggcaact
     2041 ggatttttca ttgccacctc gcggatcact tttcggaaga caatgctagg caggtgagtg
     2101 aggtgatggg gctgccggtg gagaagatgg atcatcacgc gggc
//
LOCUS       DATCBB010000873         1127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_448531, whole genome shotgun sequence.
ACCESSION   DATCBB010000873 DATCBB010000000
VERSION     DATCBB010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448531"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VII37_09615"
     CDS             <1..923
                     /locus_tag="VII37_09615"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5070038.1"
                     /translation="NIKSESHGQIDVDKLRDLVTEDVAALMITNPSTLGIFEPRIAEI
                     ADILHRKGALLYMDGANMNALTGITRPGDMGVDVMHLNLHKTFSTPHGGGGPGAGPVA
                     MKKILEPFRPTPVLIGKDGGRLALDANRPDSIGRVKAFAGNFGVLVRALAYILAYGPG
                     IRQATEDAVLNATYIRKNLEGIFELPYSLPSMHEVVFSDARQKQNGVNTMDIAKRLID
                     YGFHPYTTAFPLIVPGALMIEPTESESKEECDLFIDAMKSIAHEAATNPQLVKTAPHT
                     TRVGRLDEVCAARKPILRWKQAPARSEAAD"
BASE COUNT          268 a          360 c          286 g          213 t
ORIGIN      
        1 aaaatattaa gtccgaatcc cacggccaga tcgacgtcga taaacttcgc gacctggtta
       61 ccgaagatgt tgccgcactg atgatcacca atccctccac gttgggcatc ttcgagccgc
      121 gcatcgcgga aatcgccgac attctccacc gcaagggcgc ccttctgtac atggacggcg
      181 cgaacatgaa cgccctgact ggcattacac gaccgggcga catgggcgtt gacgtgatgc
      241 acctcaatct gcacaaaaca ttttctaccc cgcacggcgg tggtggcccc ggtgccggcc
      301 cagtggccat gaagaaaatc cttgaaccgt ttcggcccac gcctgtgctg attggaaaag
      361 acggcggacg actcgcactc gatgccaacc gcccagattc aattggccgc gtaaaagctt
      421 tcgcaggaaa ctttggtgta ctcgtccgcg cactcgctta catccttgcc tacggcccgg
      481 gaatccgcca agccaccgag gacgccgtcc tcaacgccac ttacatccgc aaaaatctgg
      541 aaggcatttt cgaattgccc tattccctgc catccatgca tgaagtcgta ttcagcgacg
      601 cgcgccaaaa acagaacggc gtaaatacca tggatatcgc aaagcgtctg atcgattacg
      661 gctttcaccc atacaccacg gcgttcccgc ttattgtgcc cggcgcgttg atgatcgagc
      721 ccacggaatc cgaatccaaa gaagaatgcg acctgtttat cgatgcaatg aagtccattg
      781 cccatgaggc ggcgaccaat ccgcagctcg tgaaaaccgc gcctcacaca acgcgagtcg
      841 gacgtctgga tgaggtctgc gccgcgcgca aaccaatcct gcggtggaaa caagcccccg
      901 cgcgttccga agccgccgac tagctcgaaa tcgcaaccaa gtacttaacc aaaaaaagaa
      961 gacccgcgac gggtatcgcc gcgggtcgaa tgagttttga ctgacaataa tgctatcgag
     1021 ccatgcgtgg cattccttcc ggtccaaacg ccaccggcgg tgcttccgcg gaccttccat
     1081 tgcgtgccgg caccgcgcgt gtaacagcag caggttccgg cctgcgc
//
LOCUS       DATCBB010000874         1158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_483576, whole genome shotgun sequence.
ACCESSION   DATCBB010000874 DATCBB010000000
VERSION     DATCBB010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483576"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1077)
                     /locus_tag="VII37_09620"
     CDS             complement(88..1077)
                     /locus_tag="VII37_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5070039.1"
                     /translation="MSLRNLKLPLSPIVSILALGLMLHSGWAVAMDTPSALLAQGRVD
                     EAIATLQERISASPNNAEAQNLLCRAFFTLGEWDRGIAACQKAITLDPNNSRYHLWMG
                     RIYGEKADKAGFLTAAGLARKVKSEFELAVELNPKNIDARTDLAEFYLEAPGIVGGGR
                     DKAEAQAQTLAGLDPAKAHWVNGRLAEKKKDFRAAENEYRAAIEASHGSANSWLNLAI
                     FYRHTVRLDEMEAAIQHAASAQIDPTGAVMDAAELLIRTKRNVPESARLLRQYLSSQS
                     TVEQAPAFKAHYLLGTVLEQQGNKQAAADEYRAALSLAKDFSLAHQALDRVSR"
BASE COUNT          260 a          332 c          301 g          265 t
ORIGIN      
        1 agagaacaag caaatgcaca gaacgcaata ggctattcat tagagtcacc ctcggggtac
       61 gaaaatttag aaaaattcca cgcctcgtca acgactgaca cgatcgagcg cctgatgcgc
      121 cagcgagaaa tccttagcca aagataatgc cgcacgatat tcatcggccg cggcctgctt
      181 gtttccctgt tgttccagaa ccgttcccag caagtaatgc gctttgaatg cgggtgcctg
      241 ttcgacggtg gattgcgaag aaagatactg gcgcagcagg cgagccgatt ccggaacgtt
      301 gcgtttggtt cggatcagaa gttcagccgc gtccataacc gcgcccgtcg gatcgatctg
      361 cgccgaggct gcgtgttgaa tagcggcctc catctcgtca agacgcacgg tgtgccggta
      421 aaaaatggcc agattcaacc acgagttcgc gctgccgtgg ctggcctcaa ttgcggcccg
      481 gtattcattt tcggcggcgc ggaaatcttt tttcttttcc gccaagcggc cattcaccca
      541 gtgtgctttg gctgggtcga ggccggcgag agtctgggct tgagcctcgg ctttgtctct
      601 tccgccaccg acgatgcccg gcgcttccaa ataaaactca gcgagatccg tgcgcgcatc
      661 tatattcttc ggattcagct ctactgccaa ctcaaattcc gatttgactt ttctcgccag
      721 gcccgccgca gtcagaaagc cggccttgtc cgctttttca ccatatatcc ggcccatcca
      781 caggtgatag cggctgttat ttgggtcgag cgtaatcgcc ttctggcaag ctgcaatccc
      841 ccgatcccat tctcccaaag tgaagaaagc gcggcaaaga aggttctgcg cctccgcatt
      901 atttggagaa gcactgattc tttcttgcaa ggttgcgatt gcctcgtcca cccgcccctg
      961 ggctaacaat gcggacggcg tatccatcgc aaccgcccaa ccagaatgca gcattaaccc
     1021 cagagccagg atagagacga tgggagataa aggaagcttg agatttctca aagacattcc
     1081 ttagtgaacc acctttacaa tcaggccgct gtgcaggggc ttggaattag ttgacgccag
     1141 ggcgaccagc gctttttc
//
LOCUS       DATCBB010000875         4242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_384049, whole genome shotgun sequence.
ACCESSION   DATCBB010000875 DATCBB010000000
VERSION     DATCBB010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384049"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VII37_09625"
     CDS             <1..917
                     /locus_tag="VII37_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070040.1"
                     /translation="LSPQELEALAIKPTENLQAYDLYLRGKRYARRQTRQDLEFALQM
                     FENAVNIDPSFALAYAACANACAMFYCNYSRDQIWVERARDASGKAVALRWDLPEVQV
                     SQAWVLYATDLHAEAVRMVQKAIERKKDCEGAYYLLCRALFSAGRYREVADAAEMALE
                     ASGEDYNVYVPILNALGALGKHEIRNNIQLRAVAAFENHLKQVPEDARARILVAGYYA
                     EVGRVDDAMRETTMAMTLRANEASILYNAACVFCLLKKKLEALDALRKAYDAGFKDPN
                     WARRDPDMALLLGDPEFERLYPASPIPN"
     gene            1129..3711
                     /locus_tag="VII37_09630"
     CDS             1129..3711
                     /locus_tag="VII37_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5070041.1"
                     /translation="MPLSAGTKFGPFVIVEPLGAGGMGEVYRAQDTRLGRDVAIKVLP
                     ESFAGDSDRLRRFEQEAQAVAALNHPNILAIHDIRTQNGAPYLVSELLEGNTLRAELE
                     QGRLGSRKIVDYAVQMAQGLAAAHEKNIVHRDLKPENVWVTKDGRVKILDFGLAKLAA
                     PGGESASMATLTSPPTEPGKVLGTVGYMAPEQVRGASVDTRTDIFSFGAVLYEMVAGL
                     QPFRRDTAAETMTAILKEEPPELSEMPQPASPGMQRIIGRCLEKKPEQRFQSAKDLAF
                     ALEALTGTGTKSAASLAIVEQQKSWKRWPGTLAAVGIPLALISGAAATWFLRPTPEPI
                     APFRQASFHRGEVIRARFGPDEKTLVYSAKLNGNPMDTYVLRAEYPEPVAAGLNGALL
                     EAVSRQGQLAVLVRPQYFAHKTFLGTLATTPMGGNAPREIMDNVSEADWSPDGQQLAI
                     IDRPGAEWRLQYPIGKILWSGSNWISDVRVSADGKRVTYFRHPPNIDDRGDVMVVEED
                     GKPRVLVGAFESLAGLAWDPSGKEIWFSASQTGQQYCIHAVTLKGKERTMHCGAAPTI
                     ILDFSASGRALVMAMQARVSMAVLEHGAKEEKDLTWLDFAYNPRLSRDGSEVLFTDQT
                     GQGSVQYGVYVRKRDGSPAVRIGENGFGSDITPDGKFALVVNADDTQRRIQIVPGGTG
                     EKKILHWDGVQPIWAQWFPDGEHILLTANIPPGKPAGLYVTDAKGATPRLIVEGAQSR
                     AGVSPDGAWIIYGRDGTRFIQSTTSTEMKDVPNFPRDQRAIAWSSGAEHLFTQAADDS
                     GVQLTRVDLKTGIAEPWQTIRPKDQVGQRPMSNPVAITPDGKWMAYYYVNELDQLYVT
                     DGLK"
BASE COUNT          950 a         1168 c         1239 g          885 t
ORIGIN      
        1 cgctgtcgcc ccaggagctg gaagcactgg ccatcaagcc caccgagaat ctgcaggcct
       61 acgacttgta tctgcgcggg aagcgctacg cgcgccggca aacgcgccag gatttggaat
      121 ttgcgctgca gatgtttgag aacgcggtga acatcgatcc aagttttgca ctcgcgtatg
      181 ccgcctgcgc gaacgcttgt gccatgttct attgcaacta cagccgggat cagatttggg
      241 tggaacgcgc gcgcgatgcc tccggcaaag ccgtggcttt gcgctgggac ctacccgaag
      301 tgcaggtaag ccaggcgtgg gtactttacg ccacggactt gcacgcggag gccgtgcgca
      361 tggtgcaaaa ggctatcgaa cgcaagaaag attgcgaggg cgcatattat ttgctttgtc
      421 gcgcgctatt ttccgcgggt cgctatcggg aagtggccga tgccgctgag atggccctgg
      481 aggctagcgg cgaagactac aacgtgtatg tgccaatctt gaatgcactc ggagccctgg
      541 ggaaacatga gatcaggaat aatatccagt tgcgggcggt ggccgcgttt gagaatcacc
      601 tcaagcaggt tcccgaggat gcacgcgcgc gcattttggt ggctggatac tatgccgaag
      661 taggtcgcgt ggatgatgcc atgcgtgaaa ccaccatggc catgactctg cgcgccaatg
      721 aggcttccat cctttacaat gccgcgtgcg ttttttgttt actaaagaaa aagcttgagg
      781 ccctggatgc cttgcgcaaa gcatacgacg ccggcttcaa agatcccaac tgggcgcgac
      841 gcgacccgga tatggcgctc ctactcggcg acccagaatt tgaacgcctc tatcccgcct
      901 cgccaatccc caattgacct gtttttcaag tcagttgatt ttcccaagaa ctggagagcc
      961 cgaagtctcg acgttgctcg ccgcgtccag cctgtcgcta acggctcaat gaatctcact
     1021 ccatcccgtg agtaaacttc ccctttatta cttcgcgtat gttgtattgc ttcgctggcg
     1081 taaacgaaat ttccgcccca actcgtctga cgatataatc cggcgcctat gccactgagt
     1141 gccggtacaa aatttggacc ctttgtaatt gtcgagccgc taggcgccgg cgggatggga
     1201 gaagtgtacc gagcgcagga tacgcggctc ggcagagatg tggccatcaa ggtcttaccg
     1261 gaatcgttcg caggtgacag cgaccggcta cggcgattcg aacaagaagc gcaagcggtg
     1321 gccgcgctga accatccaaa tattttagcc atccacgata ttagaaccca gaacggcgcg
     1381 ccgtatcttg tctccgagtt gctggagggc aacaccttgc gggcggagct tgagcaaggc
     1441 cggctgggat cacgaaaaat cgtcgattac gcggtgcaaa tggcgcaagg cctggccgcc
     1501 gcgcacgaaa aaaatattgt tcatcgcgat ttaaagcccg aaaacgtatg ggtcaccaag
     1561 gacgggcgcg tcaaaattct ggattttggt ttggccaaat tggccgcccc cggcggcgaa
     1621 tccgcatcca tggccacgct caccagcccg ccgaccgaac ccggaaaagt gctgggcacc
     1681 gtgggctaca tggcgccgga gcaagtgcgt ggcgcgtcgg tcgatacgcg cacggatatt
     1741 ttttcgtttg gagcggtgct ctacgaaatg gttgctggcc tgcagccgtt ccgccgcgac
     1801 actgcggcag aaaccatgac cgccatcctc aaggaggagc cgcctgaact aagcgagatg
     1861 ccccagccag cgtcgcccgg catgcagcgc atcatcgggc gttgtctgga aaaaaaaccg
     1921 gagcagcgct ttcagtctgc gaaagatttg gctttcgcgt tggaggccct caccgggacg
     1981 ggcacgaaat ccgccgccag cttggccatc gtagagcagc aaaaatcctg gaaacgctgg
     2041 cccggaactt tagctgctgt gggcattcca ctggcattaa tttcgggagc agccgcgact
     2101 tggtttctgc gtccgacacc tgagccgatt gcgcccttcc gacaagcttc cttccatcgc
     2161 ggagaagtga ttcgcgcgcg cttcggtcca gatgaaaaga ccctggtgta ttctgcgaag
     2221 ctaaacggca atccgatgga cacctatgtt ttgcgcgcgg agtatccgga gcctgtggcg
     2281 gccggactaa acggtgcttt gctggaagcg gtttcgcggc aaggtcaact cgccgtattg
     2341 gttcgtccac aatatttcgc gcacaaaact tttctgggaa cgctggccac cactcccatg
     2401 gggggaaacg cgccgcgaga aatcatggac aacgtctccg aagcagactg gtcaccggat
     2461 ggccaacagc tggccatcat cgatcgcccg ggcgcggagt ggcgcttgca gtatcctatc
     2521 ggcaaaattt tgtggtcggg ctccaactgg atcagtgatg tgcgcgtctc tgcggatggg
     2581 aaaagagtga cctatttccg gcatccgccg aatattgacg atcgcggaga cgtgatggtg
     2641 gtggaagaag acggcaagcc gcgtgtgctg gtcggcgcat tcgaatcgct ggccggtctc
     2701 gcgtgggatc cgtcgggcaa agaaatctgg ttttccgcat cgcagaccgg gcaacaatat
     2761 tgcattcatg ccgtcacttt gaaaggaaaa gagcgcacca tgcattgcgg agcggcaccc
     2821 acgatcatat tggatttttc tgcaagtggc cgggcgctgg tcatggcaat gcaagcgcgt
     2881 gtgtcgatgg cggtgctgga gcacggcgcg aaggaagaga aggatttgac ctggctggat
     2941 ttcgcctata accccagatt gtcacgcgac ggcagcgaag ttttgttcac cgaccaaacc
     3001 ggacaaggca gcgtgcagta cggggtgtac gtgcggaagc gcgacgggtc acccgcggtg
     3061 cgcattggcg agaacggttt cggctcggac attactcccg acggtaaatt cgcgctggtg
     3121 gtcaacgccg acgataccca aaggcgcatt caaatcgtac ccggcggcac gggcgagaaa
     3181 aaaatattgc attgggacgg cgtacagcct atctgggcgc agtggtttcc cgacggcgag
     3241 cacattcttt tgaccgccaa catcccgcca gggaagccgg ccggtctcta tgtcaccgat
     3301 gccaagggcg cgacaccgcg attaattgtt gaaggcgctc aatcccgcgc aggcgtctct
     3361 ccggatggtg cctggatcat ttatgggcgc gacggaacaa ggttcattca atcgaccacc
     3421 agcacggaga tgaaggacgt tcctaatttt ccgagggacc aaagagccat cgcatggagc
     3481 tctggcgcgg agcacttgtt cacccaggcg gcggatgact cgggagtgca gcttacccgc
     3541 gtggatctga aaaccggcat tgccgaacca tggcaaacca tccggccaaa agatcaagtt
     3601 ggtcaacggc cgatgagcaa ccccgtggcg atcacgccag atggaaaatg gatggcctat
     3661 tattacgtaa acgagttgga tcagctgtac gtcaccgacg gactaaagta acagccagtc
     3721 ccacctaaat aacagccgtc ccagacgctg gaaggcagac ccggacgcca cacatagttg
     3781 tcgcttaccg ataggacaac gggaaccccc taatgctgtc gcccgtcgcc gattgcaatt
     3841 tatagttagt catcggggca tacgcgccct cgcctgcgtt atctaaaaat tggtcttcag
     3901 gggattttat gccgcaaaac tttagccagt ggatagaact ttctctgtgc ttcgccctca
     3961 tttatgtcgt ttttcgtccg ctagtgagct tcctggacaa aattttcgag agttaaccgt
     4021 tccacttagt tccttccgga acgaacccaa ggacaatgtt ggtccagaag tttttcagtt
     4081 gctgcgggat tcgcgcgcga aactcatgga cagtaggcag ggacaggaac gtgtgaagaa
     4141 attgatattt gaagcgaaaa cggccgagtg tagcggcaag ggtgcaagga tttgcggcga
     4201 ttctttctcc ggtattcatc ccgttctaac cgctgcagca tc
//
LOCUS       DATCBB010000876         1844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_319236, whole genome shotgun sequence.
ACCESSION   DATCBB010000876 DATCBB010000000
VERSION     DATCBB010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319236"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VII37_09635"
     CDS             <1..300
                     /locus_tag="VII37_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5070042.1"
                     /translation="QKVREAPRKLLRAIPGLDLVELSHSDICCGSAGIYNVTHTEASL
                     ELLAEKMRCASATQAQVIATSNPGCLLQMRAGSEMHHTNQQVLHVIELLDQAITT"
     gene            complement(325..1518)
                     /gene="ribB"
                     /locus_tag="VII37_09640"
     CDS             complement(325..1518)
                     /gene="ribB"
                     /locus_tag="VII37_09640"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY5070043.1"
                     /translation="MAENESRFARVEEAVEEIRQGRMIVLVDDEDRENEGDLTMAAEK
                     ISPEAINFMAKYGRGLICLTLTEQRCDELHLPLMSPINTSVHGTAFTEAIDAKVGVST
                     GISASDRAITILTAIDPKTRPQDLARPGHMFPLRARNGGVLVRAGQTEASVDLSRIAG
                     LNPSGVICEIMNEDGTMARVPQLMEFCKEHDIKMLTVADLIRYRMQHERYVRRVAEAV
                     LPTRHGDFRMIAYASDVDHDQHIALVRGDLDGAEPALVRVHSHCLTGDVFGSRACDCS
                     ELVARSLAAIAKEDRGVFLYLHHTGRGFSVETSAEAGALPQIHFHSRGQLDREPARLR
                     MVQHESGIGAQILIDLGLKDIRVLTNHPKKVVALEGYGIRIADQVPLRLETAAGERRT
                     THQTL"
     gene            complement(1580..>1844)
                     /locus_tag="VII37_09645"
     CDS             complement(1580..>1844)
                     /locus_tag="VII37_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY5070044.1"
                     /translation="ISKLTPFGGFAAESAGALTLIGTAHFGIPVSTTHTITGAIVGVG
                     ATRRLSAVRWGVTRRIVIAWVLTIPGAAVVGAAVFHGIQLWLK"
BASE COUNT          383 a          596 c          468 g          397 t
ORIGIN      
        1 caaaaagtgc gcgaggctcc gcgaaaattg ttgcgcgcca ttcctggact tgatctggtg
       61 gagttgtcgc actccgacat ttgctgtggg tccgcaggca tttacaacgt cacacacacg
      121 gaagcgtctc tcgagttgct cgcggagaaa atgcgctgtg cttcagctac acaagcgcag
      181 gtgatcgcca cttcaaatcc gggttgttta ctgcagatgc gtgcaggttc ggaaatgcat
      241 cacaccaatc agcaggtttt gcatgtgatt gagttgcttg accaagccat tacgacttga
      301 attgcccctg aatgttgtca actctcacag cgtctggtgg gtagttcgcc gctcgccggc
      361 agccgtttcc aatcgcaaag gcacctgatc cgcgatccgt atcccgtagc cttccagcgc
      421 cacaaccttc ttcggatgat tggtcagcac gcgaatatct ttcaagccca aatcaatcaa
      481 aatctgtgcc ccgatgccgc tctcatgttg caccatgcgc agccgcgccg gctcgcgatc
      541 cagttgtccg cgcgaatgaa aatgaatctg cggcagtgcg ccggcctccg cgctggtctc
      601 cacggaaaag ccgcgcccgg tgtggtgcaa ataaagaaaa actccgcgat cttctttggc
      661 aatggctgcc agtgagcgcg ccaccagctc ggagcaatcg cacgccctgg aaccaaaaac
      721 atctcccgtc aaacaatgag aatgcacgcg caccagcgct ggctccgcgc cgtccaggtc
      781 gcctctgacc agcgcgatat gttgatcgtg atccacatca ctggcgtagg cgatcattct
      841 gaaatcgcca tgccgcgtcg gcaacaccgc ttccgcaacc cgccgaacgt aacgctcgtg
      901 ctgcatgcgg tagcgaatca agtccgccac ggtgagcatt ttaatgtcgt gttctttgca
      961 aaattccatc agttgcggca cgcgcgccat ggtgccgtct tcgttcataa tttcgcagat
     1021 tactcccgaa gggttcagtc cagcgattcg cgacagatcg acagatgcct ccgtctgtcc
     1081 cgcgcgcacc agcacgccgc cgttcctcgc gcgcagcgga aacatgtgcc cgggccgcgc
     1141 cagatcctgc ggtctggttt tcggatcaat cgccgtcagt atcgtaatgg cgcggtccga
     1201 agcgctgatc ccggtgctca cgcccacctt tgcgtcaatg gcttcggtga aggccgtgcc
     1261 atgcaccgac gtattgatcg gcgacatcag cggcaaatgc aattcgtcgc agcgctgttc
     1321 cgtgagcgtc aggcagatca atccgcgacc atacttggcc atgaagttga tcgcctcggg
     1381 ggagattttc tcagccgcca tcgtcaaatc gccttcgttc tcccggtcct catcatccac
     1441 gagcacaatc atgcgcccct ggcgaatttc ttcgaccgct tcctccactc tcgcaaatcg
     1501 tgattcgttt tccgccatga ccgatcccaa ctgctgcaag ggcagtttgc aacctatctt
     1561 gattttataa cccttcccat cacttcaacc aaagctgtat accgtgaaat accgccgctc
     1621 ccaccaccgc ggccccgggt atcgtcagca cccacgcgat cacaatccgc ctggtaacgc
     1681 cccaccgcac cgcagaaagc cttcgcgtcg cgcctactcc cacaatcgcg ccggtaatgg
     1741 tgtgggtcgt gctcacgggt atcccaaagt gcgcggtgcc gatcagcgtc agcgcccccg
     1801 cagattcggc ggcaaatccg ccaaaaggag tcagcttgct aatg
//
LOCUS       DATCBB010000877         1117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_60364, whole genome shotgun sequence.
ACCESSION   DATCBB010000877 DATCBB010000000
VERSION     DATCBB010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60364"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VII37_09650"
     CDS             complement(<1..408)
                     /locus_tag="VII37_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5070045.1"
                     /translation="MSANQNLCNVSATTLTRLIRTKKASAEDVITAHIERVTEVNPRL
                     NAVVQLCLERALDEARAADTQLASGTSTGPLHGVPFTIKDSFGTEGVISTGGTKGRTA
                     FVPSEDATVVGRLRAAGGILLGKTNTSELTMAFE"
     gene            complement(831..>1117)
                     /locus_tag="VII37_09655"
     CDS             complement(831..>1117)
                     /locus_tag="VII37_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070046.1"
                     /translation="EPSITHSYDTGAAWENFALQGFQQNLVVHGMEGFDYEQARKELR
                     IPEGFQVEAMAAVGKPGPKELLPEKLQQRESPNDRRKLSESVFEGPFKHN"
BASE COUNT          204 a          324 c          323 g          266 t
ORIGIN      
        1 ctcgaaagcc atagtcagtt cggacgtgtt cgtttttccg agaaggatgc cgcccgccgc
       61 gcgcaaacgg ccaaccaccg tggcatcctc gctgggcaca aacgcagtgc gacctttggt
      121 cccgccagtg ctgatgacgc cctcggtgcc gaagctatct ttgatcgtga acgggacccc
      181 gtgcagaggc ccggtcgacg tgccgctggc cagttgcgta tccgcggcgc gcgcttcatc
      241 cagcgcgcgt tcgaggcaca gctgcacaac ggcgttcagc cgcgggttca cctcggtgac
      301 cctttcgata tgcgcggtaa tgacgtcttc cgccgaggcc tttttcgtgc ggatgagccg
      361 cgtcagcgtg gtcgccgaga cgttgcagag attctggttg gcgctcacga tttgttggag
      421 cctactttcc gcggccacca agttcgcgca agattctttc cttggcgctc gatggtaatg
      481 acacgtcgga gctttggtcc gcgtgctcgg gaaactttga cgctgttttg cggaggtaac
      541 ggaggtgctc ttcgtaacgt tggcaccagc agcagatcag gtaatgcaga cgcttcctga
      601 tccgcatcgt gagcggaagg ggtcggtcca tggaatccga cagaatgcgc accatctcac
      661 ggcacttcgg ggtgcttgcc gatgaagatg acgagcttgg tctgccacca gctacgtctc
      721 gcgaggactg atgggtttcc aatcggcgat ggattgtacg gttcttctgg cccgcattaa
      781 tctcaaaaag atttgatcga tgcgcagctt ggcagggcgg attcctttat tcagttatgc
      841 ttaaacggcc cttcgaaaac gctctccgag agtttgcggc gatcattcgg actttcgcgc
      901 tgttgcaatt tttcagggag taattccttc ggtcccggct tgccgaccgc agccattgcc
      961 tcgacttgaa atccctcggg aatccgcagc tccttacgcg cctgttcgta atcgaaccct
     1021 tccattccgt gcaccacgag attctgctga aagccctgga gcgcaaaatt ctcccaggct
     1081 gctccagtgt cataggaatg cgtgatcgat ggttcgt
//
LOCUS       DATCBB010000878         1237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_319250, whole genome shotgun sequence.
ACCESSION   DATCBB010000878 DATCBB010000000
VERSION     DATCBB010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319250"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VII37_09660"
     CDS             complement(<1..685)
                     /locus_tag="VII37_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5070047.1"
                     /translation="MKVRLPVRNAILQRKTYEAPAEDRLNKMRLDFNENTTGCGPAVL
                     RALRKLTPQQLAMYPEYAKPTRRIAAYFGVSEHELLLTNGGDDALRVFFDAFVEPHNS
                     ILICEPTFPMYRYYAEIAGARVETLRYDAEMEFPLDDFVSALRKQPVVIFIANPNNPT
                     GTLLPLAAIKQILLAAPKTVVVIDEAYAEFSGVTVLPWIRKYPNLFVTRTFSKAAGLA
                     GLRLGAAMAC"
     gene            complement(682..>1237)
                     /locus_tag="VII37_09665"
     CDS             complement(682..>1237)
                     /locus_tag="VII37_09665"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5070048.1"
                     /translation="VDDGDPAWIAADLLAQAEHAPDAGGFLVTTSRDLAARTQVEIQK
                     QLRALPKTNPAQQSSVRSGAILLAPSMERACEFVNRFAPEHLSLPQNTDELLQKVQAS
                     GTVFVGPWSAQAFGDYASGSNHVLPTAGWARARGGLSTADFVKSISVQRISETGFARL
                     AQDVQILARAEGLLAHANAIEVRR"
BASE COUNT          287 a          377 c          317 g          256 t
ORIGIN      
        1 gacaagccat cgcggccccc aatcgcagtc cagccagacc cgcagctttc gaaaaagtgc
       61 gcgtcacaaa caagttcgga tatttccgaa tccacgggag caccgtcacc ccggaaaact
      121 ccgcgtaagc ttcatcgatt acaacgacgg tcttgggtgc cgcgagtaag atttgtttga
      181 tcgcggctag cggcaagagc gtccccgttg gattgttggg gttagcaata aaaatgacca
      241 ccggctgttt tcgtaacgcc gaaacgaaat catccagtgg aaattccatc tcagcgtcgt
      301 agcgcagagt ttcaacccga gccccggcaa tctccgcgta gtaccgatac atcggaaaag
      361 taggctcgca aatcaaaata gaattgtgag gttcaacgaa cgcatcgaaa aatacccgca
      421 acgcgtcgtc tcctccgttg gtaagcaaca attcatgctc cgaaactccg aagtacgcag
      481 cgatgcgtcg cgttggcttg gcatattccg gatacatcgc caattgctgc ggagtaagtt
      541 tcctgagcgc gcgcagtacc gccgggccac accccgtagt gttctcattg aaatccagcc
      601 gcattttgtt cagtcggtcc tctgcggggg cctcataggt cttgcgctgc aaaatggcat
      661 tgcgaaccgg cagtcgcacc ttcatcgacg cacctcgatt gcattcgcgt gcgccagcaa
      721 tccttcggcc cgtgccagaa tttgaacgtc ctgcgccagt cgcgcaaatc ccgtctcgct
      781 gatgcgctgc acgctgatgc ttttcacgaa gtccgcagtg ctgagtccgc cgcgtgcccg
      841 tgcccatccc gcagtaggca aaacgtgatt acttccactc gcgtaatcgc caaatgcctg
      901 cgcgctccac ggaccaacaa aaaccgttcc cgaagcttga accttttgga gcagctcatc
      961 ggtgttctgc ggcaaactca agtgctccgg cgcgaagcgg ttcacaaact cacaggcccg
     1021 ctccatcgaa ggagccaaca aaattgcgcc gctccgcaca gaagactgtt gtgcgggatt
     1081 cgtcttcggc aatgcgcgca attgcttctg aatctcaact tgagtgcgag cagctagatc
     1141 acgagaagtt gtaactagga aacctccagc gtcgggagcg tgctcggctt gagccagcaa
     1201 atccgccgcg atccacgccg ggtcgccgtc atctacg
//
LOCUS       DATCBB010000879         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_384096, whole genome shotgun sequence.
ACCESSION   DATCBB010000879 DATCBB010000000
VERSION     DATCBB010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384096"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VII37_09670"
     CDS             <1..912
                     /locus_tag="VII37_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5070049.1"
                     /translation="DAAQAAQQASQQATQAAMQASQQASQQAMEASQQAAQSAQIAMQ
                     NTTPPPAGPSLTATPKFSLKQGAFSTPVTVKIQDRTRGAIIYYTTDGWTPTINSPRYK
                     GPITIDSNTTIQAIAIAPYALRSWVATAQYAVQPSKAPSSPSPVSSAVQVTPAIPADG
                     KFVLPKGTPVRLVFASAVNSSTATVGDKISLTLADDISVGTEIVAKKGWPASGTVIQV
                     DKAGAGGLPGQVDFEVNFLDANGTTVKLLGSAAKEGQAKPPNAAVLIPIVGPFTLFKP
                     GTDAEISPGAIFTASVAEDTALAPAVN"
     gene            complement(937..1260)
                     /locus_tag="VII37_09675"
     CDS             complement(937..1260)
                     /locus_tag="VII37_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070050.1"
                     /translation="MPVTILGCLFIVPGLVTLAYHLSQRPVDRWAALMVFLEIIAIVG
                     GVFLLKGRNWARWLMIGWVGFHVGVSAFKSLSDCIAHAVLFVAVAYFLLTPPDSKYFQ
                     ASPSE"
     gene            complement(1318..1728)
                     /locus_tag="VII37_09680"
     CDS             complement(1318..1728)
                     /locus_tag="VII37_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5070051.1"
                     /translation="MSYADHVDGEINDQYRSITGALRFLKNIILLPGCGQFVPAASFV
                     LRQPGSDELVAAVLTSEVSPGVGHTTQICVVPGFQGHGLGRMLMQKSSDALRALKFKE
                     LTLTVTSENRTAVQLYDRLGFHTIKSFTAGVWPR"
     gene            complement(1759..>2122)
                     /locus_tag="VII37_09685"
     CDS             complement(1759..>2122)
                     /locus_tag="VII37_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070052.1"
                     /translation="LEARALAGSVAFENGNAAGYSFYVMEEQKGLIGGLYVSAQFPQS
                     AIAQRLLEETLFSMRAIPHLERIEAQLMPFSGPLDTPLRDQGFSLYTRQFMLLDLQKA
                     AKTNSNNSAALRIDRWND"
BASE COUNT          521 a          664 c          511 g          426 t
ORIGIN      
        1 gatgctgcac aagctgcgca gcaggcctcg caacaagcca cgcaggcggc catgcaagct
       61 tctcaacaag ccagccagca agccatggag gcttcgcaac aagccgcaca gtcggcgcaa
      121 atcgctatgc agaacaccac accgccgccc gcgggtccgt cattaacagc gactcccaag
      181 ttctcgctga agcaaggcgc cttttccacc ccggtcacgg taaaaatcca ggaccgaact
      241 cgcggcgcga tcatttacta caccaccgac ggttggactc ccacaatcaa ttcgcctcgc
      301 tacaaaggtc ccatcaccat cgattccaat actacgattc aagctattgc gattgcacct
      361 tatgctctac gaagttgggt ggccaccgcc caatatgcag tccaaccctc gaaagcgcca
      421 tcttccccct caccggtttc gtctgccgtg caagtcactc cagccatacc ggcggatggc
      481 aaattcgtgc ttcctaaggg caccccggtg cgactcgtct ttgcctcggc ggtaaactcc
      541 agcaccgcca ctgttggtga caaaatttct ctcacgctcg cagatgacat atcggttggc
      601 accgagatcg ttgccaaaaa aggttggccg gcctcaggca cggtgattca agtggacaaa
      661 gcgggggctg gtggtctgcc tggtcaagtc gatttcgaag tgaattttct cgacgccaac
      721 ggcaccaccg ttaagctgct cgggtccgca gcgaaagaag gccaagccaa accacccaat
      781 gccgccgttt taattcccat cgtaggtccg ttcactcttt tcaagcctgg aaccgatgcg
      841 gagatcagtc cgggcgctat ttttaccgcg tcggttgcag aggataccgc gctcgcaccg
      901 gccgtcaact aagcgttaat gctcagacag tgcgcttcac tctgacgggg aagcctgaaa
      961 atatttggag tcaggcggtg taagcagaaa ataagctaca gctacgaaca gcacagcatg
     1021 tgcgatgcaa tctgacagcg acttgaacgc gctcacccca acgtgaaagc caacccaacc
     1081 gatcatcagc cagcgagccc agttgcgccc tttgagcagg aacactccac ccacgattgc
     1141 aattatctcg agaaaaacca tgagcgcggc ccaacgatcc accggccgct gcgaaaggtg
     1201 gtaagccaag gttactagcc cgggtacgat aaacaagcaa cctagaatcg ttacaggcat
     1261 gggccgtttc atagcatcgc gcaattaacc aagctttcac cctaaatcgc aaatacctta
     1321 gcgtggccag actccagccg taaacgactt aatggtatga aatcccagcc ggtcgtaaag
     1381 ctgcacagca gtgcggttct cggaagtcac tgtcaatgtc aattccttaa actttaaagc
     1441 tcgcagcgca tcactcgatt tttgcatcag catgcgcccc agtccgtgcc cttggaatcc
     1501 gggaaccacg caaatctgcg tggtgtgccc cactcccggt gatacttcgc tcgtcagtac
     1561 cgcggccacc aactcgtcac tgcccggctg ccgcaaaacg aacgacgccg ccggcacgaa
     1621 ttgcccgcac ccgggcagca aaataatatt tttcagaaag cgcagcgccc ccgtgatgct
     1681 gcgatactga tcattgattt ctccatccac atgatcggcg tacgacaaat aaattaactt
     1741 cgcgcacggt tcgaaatatc aatcgttcca gcgatcgatt cgcagcgcgg cggaattgtt
     1801 ggaatttgtc ttagccgcct tctgcaagtc cagcagcatg aactggcgcg tatacaggct
     1861 gaatccttgg tcgcgcagcg gtgtatcgag cggcccgctg aagggcatca gttgcgcctc
     1921 aatgcgctcc aggtgcggaa tagcccgcat ggaaaacagc gtctcctcca gcaatcgctg
     1981 tgcaatagcg gattgcggga attgcgccga aacgtacagc ccgccgatca atcccttttg
     2041 ttcttccatc acataaaacg aatatcccgc ggcatttcca ttttcaaacg ccacgctccc
     2101 tgctaaagcc cgcgcctcca ga
//
LOCUS       DATCBB010000880         1473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_448622, whole genome shotgun sequence.
ACCESSION   DATCBB010000880 DATCBB010000000
VERSION     DATCBB010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448622"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>1473
                     /gene="glyS"
                     /locus_tag="VII37_09690"
     CDS             32..>1473
                     /gene="glyS"
                     /locus_tag="VII37_09690"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY5070053.1"
                     /translation="MLPRAAEELRTNITKLLTAENLMEGVAVETFCGPRRLSAWVRGL
                     TAKQDDVTSDVTGPPKSVAFDNAGAPTRAALSFAEKQGLRVEDLHRIQTPKGEYLAAR
                     QVRRGRTAEEILCEILPRTIHDLTWPRSMTWTGIDGARFIRPIRWLLAVLDGRPLKFS
                     YGGVSSGDKTFGHRFIGKGELTIRNFEEYEKKLGANGVIVRPEARREKIERELAQLSR
                     KNGIQIHKDAALENLVCYLNEYPTVIEGEFDSEFLNLPDEILVTVMRDHQKYFAVENT
                     RGELAPKFLAVINLARDSRGLVRAGHERVLRARFADAKFFWAADQKHPLSDNLAKLER
                     VTYESRLGSYRDKVERVRSIARWLTEQWYSSGMIEAHVAEADHAAELAKCDLATEMVR
                     EFPELQGVVGGLYARAQGEPDEVADAIYDHYRPVGLTDPIPRNVTGCAVALADKLDSI
                     AGGFTVGVVPTGSSDPYALRRAALGIVKII"
BASE COUNT          329 a          368 c          467 g          309 t
ORIGIN      
        1 cgagattggc tgcgaggaaa ttccggcagg gatgttgcca cgcgccgcag aagagctacg
       61 cactaacatc acgaaattac taaccgcgga aaatctgatg gagggcgtcg cggtcgagac
      121 attttgcggt ccgcgccggc tctcggcctg ggtccgtggg ctcacagcga agcaggacga
      181 tgtcaccagc gacgtcactg gaccgccgaa atctgtggct tttgataatg ccggcgcacc
      241 tactcgcgcg gccttgagct ttgcggaaaa gcagggcttg cgcgtggagg acctgcaccg
      301 catacaaact ccaaaaggcg aatatctggc ggcgcgtcag gttcggcgtg gcagaaccgc
      361 ggaagaaatc ttgtgcgaga ttttgccgcg aaccattcac gatttaacct ggccgcggtc
      421 gatgacctgg acgggaatcg atggcgcgcg attcattcgt cccattcgct ggctgttagc
      481 agtgctggat ggacgtccgc tgaaattttc ttatggcggt gtttcctcag gcgataaaac
      541 ttttgggcat cgcttcattg gaaaaggcga actgacgata cggaattttg aggagtacga
      601 gaagaagctc ggcgcgaacg gggtgattgt acggccggag gcgcggcgcg aaaaaattga
      661 aagggaattg gctcagctct cgcgcaagaa tggcattcag attcataaag acgcggctct
      721 cgaaaacctc gtgtgttact tgaacgagta ccccaccgtc atagaaggag aatttgatag
      781 cgagtttttg aatttgccag acgagatcct cgtgacagtg atgcgcgatc atcagaaata
      841 ctttgcggtg gaaaacaccc gtggagaatt agctcccaaa tttttagcgg tcatcaatct
      901 ggcaagggat tcgcgtggtc tggtacgtgc cggccatgaa cgagtgctgc gcgctcgatt
      961 tgccgatgcg aaatttttct gggcggccga ccagaagcat ccactgtcgg ataatttggc
     1021 aaagttggag cgggtcacct acgaatcgcg gctgggcagc tatcgcgaca aggtagaacg
     1081 ggtacgctcc atcgcgcgct ggcttaccga gcagtggtac agcagtggga tgattgaggc
     1141 gcatgtggcc gaggccgacc acgcggctga attagcgaag tgcgatctgg ctaccgagat
     1201 ggttcgcgag tttccggaat tgcagggcgt cgtcggcggg ctctacgcac gcgcgcaagg
     1261 cgagcccgat gaagtggctg acgcgattta tgatcattac cggccagtgg gattgacgga
     1321 tccgatcccc cgaaacgtga ccggctgcgc cgtggcactg gcggataagc tggattccat
     1381 tgccgggggt tttacggttg gcgtcgtgcc gacgggctcg agcgatccgt acgcgctcag
     1441 aagagcggcg ctcggcatcg tcaaaattat tat
//
LOCUS       DATCBB010000881         1512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_189315, whole genome shotgun sequence.
ACCESSION   DATCBB010000881 DATCBB010000000
VERSION     DATCBB010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189315"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..>1512
                     /locus_tag="VII37_09695"
     CDS             186..>1512
                     /locus_tag="VII37_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5070054.1"
                     /translation="MFRRGERWEAISSAEFLRRVAGLSSAFVELGVKTGDRVAIFSAY
                     RPEWHTADFAITGAGGVSVPIYFNESPQRMVYILNHSEARIIFVSGARQLEKLLACRS
                     ELLTVEHIVAADAGPEAPPECLRYETLVAGAGATEIASYRLRASQVQPSQLSSLIYTS
                     GTTGEPKGVTLTHTNFCSNVTDTHLQIRFDTTTDLALSFLPLAHVYGRMIDYSFLFHG
                     ITVAYVEAVEQVAQAMLELRPTTMAAVPRVFEKIYTRIVEQGSKATGIKRKLFDWSMK
                     VASQSAKWRTGEGQAPLTVRLQWSLANALVYSKIRAGTGGRLHLVMSGGAPLSKNLTE
                     FFWTIGIPIYQGYGLTETSPVLTSNYPHNRAGSSGKAIANVELRIADDGEILARGPCV
                     MQGYYKNPEATREVLSADGWFSTGDIGYLDADGYLFITDRKKDLIKTAAG"
BASE COUNT          334 a          383 c          453 g          342 t
ORIGIN      
        1 agacagcatt tgcacgacgg cgacttgttg cctgaactgc actctttggt ttatcgaagc
       61 acgaccggaa ggtacaattg cgccctgtaa atagctagag tacagtcgaa atcgtgatgg
      121 attacggcac acttccctcc agatttttga gcgccatcga ccgatcacca aatccacggg
      181 ctcaaatgtt ccgccgtggc gaacgttggg aagcgatatc gtcggcggaa ttccttcggc
      241 gcgtcgcggg actttcgagc gcgttcgtgg aactaggcgt gaaaacggga gaccgcgtag
      301 caatattttc ggcgtaccgg ccggagtggc ataccgcgga tttcgcgatt accggggccg
      361 gcggggtcag cgtaccgatt tatttcaatg aatcgccgca gcggatggtt tatatcctga
      421 atcattccga ggccagaatt atttttgttt ccggagcgcg gcaattggaa aagttgttgg
      481 cgtgccgctc agaactgctc actgtagaac acatcgtggc cgccgacgca gggcctgaag
      541 ccccccctga atgtttgcgg tatgaaacgc tggttgcggg tgccggcgcg acggagatcg
      601 cgtcgtaccg gctgcgagca tcgcaggtgc agccgagcca gctttcttcg ctgatttata
      661 cgtcaggcac taccggtgaa cctaaaggcg tgacgctaac gcacacgaat ttctgttcga
      721 acgtcacgga tactcacctg caaattcgat ttgatacgac cactgatctg gcgctgtcat
      781 ttttgccgtt ggcccatgtt tacggccgca tgatcgacta ctcgttttta tttcacggta
      841 ttaccgtggc ttacgtggaa gccgtggagc aggttgcgca agcaatgttg gaactgcgtc
      901 ctaccacgat ggccgctgtg ccgcgcgtgt tcgaaaagat ttatacgcgg atagtggagc
      961 agggaagcaa ggccacagga attaaacgca aactgtttga ttggtcgatg aaagtagcca
     1021 gccagtccgc taaatggcga accggcgaag ggcaagcgcc tttgactgtg cggctccaat
     1081 ggtcgctggc gaacgcgctc gtatattcga agattcgcgc gggaacggga gggcggctgc
     1141 atctggttat gtccgggggc gccccgctct caaaaaatct gacggagttc ttttggacga
     1201 ttggcattcc gatctatcag ggctacggat tgacggagac ttcgccggtg ctgacgtcga
     1261 attatccgca caaccgtgca ggcagctccg ggaaagccat cgcgaatgta gagctaagaa
     1321 tcgccgacga cggcgagatt ctagcgcgcg ggccctgcgt gatgcagggg tactacaaga
     1381 accccgaggc gacccgggaa gttcttagcg cggatggttg gttcagtaca ggggacatcg
     1441 gctacctgga tgcggacggt tatttattta ttactgaccg caagaaagat ttgattaaga
     1501 ctgctgcggg ta
//
LOCUS       DATCBB010000882         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_286852, whole genome shotgun sequence.
ACCESSION   DATCBB010000882 DATCBB010000000
VERSION     DATCBB010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286852"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VII37_09700"
     CDS             complement(<1..734)
                     /locus_tag="VII37_09700"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY5070055.1"
                     /translation="MRIAIDSGGTFTDCVFVRDGKLQIIKVASRPDDPAQAIVEAVHL
                     AVARASSNIETHDLVCGTTVGTNALLERRGGRVALITTSGFEDVLEIGRQARAKLYDL
                     FLQKTEPLAPSSRRLGAKERLASDGSIVQPLSTAEISHVLRQIKKVNPDSVALCLLFS
                     FRNPLHEKRIAANLRSVGYAVSVSHEILPEFREYERTSTTVINAYLVPVMSSYLRDTT
                     VRVAAVFSQQAGKTSEKNRRQSSTLSR"
     gene            936..1571
                     /locus_tag="VII37_09705"
     CDS             936..1571
                     /locus_tag="VII37_09705"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY5070056.1"
                     /translation="MAAELLRINAHSPEPNVLRYAADFLTRGRVVAVPTDTVYGLAAD
                     PFNLSAVEEIYKVKGRPEARALPILVNSIDQALLLARDVPENFLRLAHEFWPGALTLV
                     VDAGHRLPLKVTANTGRVALRLPRSAVIAALIDEFDGPITGTSANLSGHPSCISGVQV
                     QEQLGDRLPLILDVGDLGTSLASTIVVLRGDNWKIVREGIIPGADIERFVS"
     gene            complement(1622..2062)
                     /locus_tag="VII37_09710"
     CDS             complement(1622..2062)
                     /locus_tag="VII37_09710"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5070057.1"
                     /translation="MDSTFGITTTPSPKLSAALLAIRLASALAFLYHGAAITFGAFGG
                     PGPEKFAAFTHMPLIAGYLVGLAQLAGGLAMLTGVLIRVGAVCIIIVMLGAIFLVHLP
                     HGFDIGHGGMEYALTQLLVAFAILLTGPGAYSLAGSLPASIRRL"
BASE COUNT          567 a          685 c          719 g          578 t
ORIGIN      
        1 cgcgagagtg ttgaagattg ccggcggttt ttttcggagg tttttccagc ttgctgcgaa
       61 aaaactgcgg cgacgcggac agtggtgtcg cgaagatagc tgctcatcac cggcacgagg
      121 tacgcattga tcaccgttgt ggaggttcgt tcgtattcgc gaaattccgg aagaatttcg
      181 tgcgaaacgg aaacggcgta acccaccgat cttagattgg ctgcgatgcg cttttcgtgc
      241 agcggattgc ggaatgaaaa cagcaagcac agcgcgactg aatctggatt tactttcttg
      301 atttggcgca acacatggct gatttcggct gtgcttaaag gttggacgat ggaaccgtca
      361 gaagccagcc gctcctttgc gccgagacgt cttgacgacg gcgccagcgg ttcagtcttc
      421 tgcaagaaca gatcgtataa tttcgcccgc gcctgcctgc caatctccag cacatcttca
      481 aagccggagg tggttatcag cgcaacgcgc ccgccgcgac gctcaagcag agcatttgtg
      541 cctacggttg tgccgcaaac gagatcgtga gtctcaatgt ttgatgaagc gcgtgcgacg
      601 gccagatgta ccgcttcgac gatggcttgc gcagggtcat cgggccgcga ggcaactttt
      661 atgatttgca atttaccgtc acggacaaag acgcaatcgg taaaagtgcc gccgctgtcg
      721 atggcaatgc gcatgagttg gtgattctac attacaggta gtgctgtccg tcacagcgcc
      781 taagaaacgt gatgagggct gctggcgtta aacaattctt cggttatcga actgtattta
      841 ttgcacagac gtgcaccctg tggtagcgtc ccggcagcaa gatcggcgac gcgaagcgct
      901 gcgtaagcat tttcaacttc gcccaaggcc cgaaattggc agcagaattg ttgcgcatca
      961 acgcgcattc cccggaacct aacgtgctgc gttatgcagc agattttctc acgcgtggtc
     1021 gtgtggtggc ggtgcccaca gacacggtct acggccttgc tgccgatccc tttaacttgt
     1081 ccgcggtgga agaaatttac aaagtgaaag gccgcccgga agcccgggca ttaccgattc
     1141 ttgtgaactc gattgatcag gccctgctgc tcgcgcgtga cgtgcctgag aattttctgc
     1201 ggctggcgca cgagttctgg cccggggcgc tcacactagt tgttgatgct gggcaccgtc
     1261 tgccattaaa agtcacagcc aataccgggc gtgttgcttt gcgtttgccg cgaagcgcgg
     1321 taattgccgc gctcatcgat gagttcgacg ggccgattac cggaaccagc gccaaccttt
     1381 cgggacaccc ttcctgcatc agcggggtgc aggttcagga acagctaggc gaccggcttc
     1441 cgcttattct cgacgtcgga gatctaggaa cctcactcgc gtccaccatc gtggtgctgc
     1501 ggggcgacaa ctggaagatt gtgcgtgaag ggattattcc tggcgcggac attgaaagat
     1561 ttgtttcctg agtgctagcc cgcgtcccga atttgtgatc cgcgagcggc gtctgattaa
     1621 attacaatct gcggatcgat gcaggcagtg agcctgccaa cgaatacgct cccggccctg
     1681 tcaacaatat tgcaaaagcc accagcagct gagtgagcgc gtactccatt ccgccgtgcc
     1741 caatatcgaa tccatgcggc aaatgcacga ggaaaattgc gcccagcatt acgatgatga
     1801 tgcacactgc gcccacacgt atcaaaaccc cggtcaacat tgccaatccg ccggcaagct
     1861 gcgccagtcc cacaaggtaa cctgcgataa gcggcatatg cgtgaacgca gcgaatttct
     1921 cagggccagg cccgccaaac gcaccaaacg taatagcggc gccgtgatac aaaaacgcca
     1981 gggcgctggc gaggcgaatt gccaagagag ccgcactaag tttcggcgac ggagttgtgg
     2041 tgattccgaa ggtgctgtcc atatcgagtc tccctctttg atcttgaatg agctacggtt
     2101 gccgctggct acagcctata gaataaattt ttggtcacgg gtttttcggg cgacgcaatc
     2161 taaaggcggc tcgaggcttt ccctgatatc agtgcatcga cggtaaacta aggtaaagct
     2221 acgtgggtaa tactacgaag gttcaaggtg aagcaagacc caggttcata actcagctgc
     2281 caccctgggc caccctccgc ggttttcgga tttgcaattt tagggtgggc tgggagttgt
     2341 ggcgattttg cggagcaagg aaaacatatt acgcgcttca taatttcggg gacgctcagg
     2401 gcttagagat tacttttccg cgtatacttt ttggcgatgc cgcaaactca acgcacccca
     2461 cgaacgtcca gcgaattgcc taaagtcccg cactggcctg cgaccattgc gatattggcg
     2521 gacgcttgtc tgtatctggc gctgccgcc
//
LOCUS       DATCBB010000883         1648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_417448, whole genome shotgun sequence.
ACCESSION   DATCBB010000883 DATCBB010000000
VERSION     DATCBB010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417448"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VII37_09715"
     CDS             complement(<1..463)
                     /locus_tag="VII37_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY5070058.1"
                     /translation="MSDTRTNGNQSLQKSLPSSYYWSEEIFSREKERIFFREWFCAGR
                     EEQLPNAGDFLVLDIVGESVLVVRTKEGSLKAHYNVCRHRGARLCTSDAEPLVSDDVH
                     LKGGVLGTTGIRCPYHQWTYALDGKLLHAPHFTDGPAFSKSDFSLYPVGLQT"
     gene            complement(510..>1648)
                     /locus_tag="VII37_09720"
     CDS             complement(510..>1648)
                     /locus_tag="VII37_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5070059.1"
                     /translation="VGKRFRKISIKQIAELISFLTGSLGEFLDRNYESEKIKTLFLAN
                     NVYGKHGGPYQQGSAIGLLFHLLSGGEHEQQGFYGHVMGGMGAITQAMASAARKFGVE
                     IKSNSPVAQIDVRNGRLRGVILEDGTELRSRIVLSNADPKRTFLTLVPASELPVDFLQ
                     AVRGIKMDGPCAKVNFVLSEEPRFTGTPPNAPPLERTLYTLVRSLEFAERCYDIAKFG
                     EIPEELWVDCVVASNADGSLAPPGRHILTCFVQYVPYRLRAGDWDSNRELLGDRVVKK
                     ISEFAPNLPNAIVARQVLTPLDLERTYGLTEGNIFHGELNLEQLFFMRPVAGWSQYRT
                     PITGLYLCGAGAHPGGGVTGAPGHNAAKQALRDWKKLRKQPLPA"
BASE COUNT          391 a          446 c          432 g          379 t
ORIGIN      
        1 aagtttggag tccaacggga taaagcgaga agtcagattt cgaaaaggca ggaccatccg
       61 tgaagtgcgg tgcatgaagc aatttgccgt cgagggcata ggtccactga tgataagggc
      121 agcgaatacc cgtagtgcca agcaccccac cctttaggtg aacatcgtca gaaaccaggg
      181 gttcagcgtc ggaagtacaa agccgggcgc cacggtgacg gcagacgttg taatgagctt
      241 tgaggctgcc ctctttggtc ctgacgacca aaacactttc gcctacgata tcgaggacca
      301 ggaaatcgcc ggcatttggg agttgttctt cgcgacccgc gcaaaaccac tctctgaaaa
      361 agatgcgctc tttctcccgg ctgaaaattt cttccgacca gtagtaggag ctgggcagag
      421 atttctgcaa tgattgattt ccgttggtcc tggtgtcgct catgaagcct cagtcgtctt
      481 gcgccaagaa tatctcaaga tcgtgccggt taagcaggca gtggctgttt gcgaagtttt
      541 ttccaatcgc gtagcgcttg cttcgccgcg ttgtggcctg gcgcaccggt taccccacca
      601 cctggatgcg cgcccgcgcc gcacaaataa agcccggtaa taggggtgcg gtattgcgac
      661 cagcccgcca caggcctcat aaaaaataat tgttcaagat ttaattcgcc gtgaaatata
      721 tttccttcgg tgagcccgta ggtgcgctcc agatcaagcg gtgttaaaac ctgacgcgcg
      781 acaatggcat tcggcaagtt cggcgcaaac tctgagattt tcttcactac acggtcaccc
      841 aggagttcgc gattcgaatc ccagtcgcct gctcgcaagc gatacggaac gtattgcaca
      901 aaacaagtca ggatgtggcg gcccggcggc gcgagtgagc catccgcgtt agaggccacc
      961 acgcaatcga cccacaattc ttctggaatt tcgccgaatt tcgcgatgtc gtagcagcgc
     1021 tcggcgaatt ccagcgaccg gaccagcgta tacagcgttc tttccagcgg cggagcattt
     1081 ggaggtgtgc cagtgaaccg cggctcttcg ctgaggacga agtttacttt cgcgcagggg
     1141 ccgtccatct taattccgcg gacggcctgc aaaaaatcga cgggcaattc cgacgcagga
     1201 accagcgtca gaaaagtccg tttagggtcg gcattggaaa gcactattct tgagcgcaac
     1261 tctgtgccat cttccaaaat cactccacgc aaccggccgt tacgaacatc aatttgagca
     1321 accggcgaat tgcttttaat ttccacgcca aattttctgg cggcgcttgc catagcctgc
     1381 gtaatggcgc ccatgccacc cataacatgg ccgtaaaatc cttgttgctc gtgttcgccg
     1441 ccgctcagca gatgaaacaa cagtccaatt gcggatccct gctggtacgg tccgccgtgt
     1501 ttaccgtata cattgttggc gagaaaaagc gtcttgatct tttcggattc atagttgcga
     1561 tcaagaaatt cgcctagact cccggtcaaa aaggaaatta gttccgcgat ttgcttgatc
     1621 gaaatttttc ggaagcgctt gcctacat
//
LOCUS       DATCBB010000884         1044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_125276, whole genome shotgun sequence.
ACCESSION   DATCBB010000884 DATCBB010000000
VERSION     DATCBB010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125276"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VII37_09725"
     CDS             <1..459
                     /locus_tag="VII37_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070060.1"
                     /translation="PTVLNNTYNQHVNGAIFNAAAFGPPSVGTDVFSNANVAKRNLLW
                     GPGTWGVNLGVHKDFHVTERLTFQLGADVDNLFNHPLLSPDANAGGGGGVFALVGDFN
                     IRVDQTTGKLLPIAASDITPNPFFGRLINSFTQEGIDSRRTIRLRARITF"
     gene            complement(509..>1044)
                     /locus_tag="VII37_09730"
     CDS             complement(509..>1044)
                     /locus_tag="VII37_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5070061.1"
                     /translation="STLVVGAIGAVTQHFILFAKDQGYSTATASRFFTGLLTASLGGR
                     VLVGYLADRFRKKNTMALFYCMLSLSILLLSVSHRPAMIWGFVLLFGFAMGADYMLIP
                     LVTAECFGTASLGKILALIIMGYSLGQWGAPWIAGRIFDARHSYELAWNIIAIAGLVG
                     AAAIFAVSPRSEKKLTS"
BASE COUNT          251 a          306 c          264 g          223 t
ORIGIN      
        1 cccacggtgt tgaataacac ctacaaccaa catgtgaacg gtgcaatttt taatgctgct
       61 gccttcggtc caccttcggt cggcaccgac gttttcagta atgccaatgt tgccaagcgt
      121 aacttgctct gggggccagg aacctggggt gtgaatcttg gcgtgcacaa agacttccac
      181 gttacggagc gtctgacatt ccagctcggt gccgacgtcg acaacctttt caaccacccg
      241 ctgctttcgc ccgatgcgaa cgccggaggc ggtggcggag tgtttgcctt agtcggggac
      301 ttcaatattc gcgtggatca aaccacaggc aaattactgc cgattgccgc ctcggacatt
      361 acccccaatc cttttttcgg gcgattgatc aacagcttca cacaagaggg catcgacagc
      421 cgccgcacca ttcggctgcg cgccagaatc actttctaac atctgcagcc tgattaattg
      481 tttttgcgat gtggcgggtg ccacgcgatt aagaggttag cttcttttca gatctgggcg
      541 agaccgcaaa gatcgcggcc gcccctacca atcccgctat agcaataatg ttccatgcca
      601 attcgtagct gtgtcgcgcg tcaaaaatcc ttccggcgat ccacggcgcg ccccactggc
      661 ccagcgaata tcccatgatg atgagcgcca gaattttgcc gagcgaagct gttccaaaac
      721 attccgcagt gaccagcggg attagcatat agtccgcgcc catcgcgaaa ccaaacagca
      781 gcacgaatcc ccagatcatc gcaggccggt gagaaacact gagcagcagg atggacaagc
      841 tgagcatgca ataaaacaaa gccatggtgt tcttttttcg aaagcggtcc gccaggtaac
      901 ccaccaatac gcggccgccc aggctagccg tcaacaagcc ggtaaagaat cgagaggctg
      961 tcgccgttga gtatccctga tccttcgcga acagtatgaa atgctgggtg actgcgccaa
     1021 tcgcgccaac cactaaagtt gagc
//
LOCUS       DATCBB010000885         1724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_448704, whole genome shotgun sequence.
ACCESSION   DATCBB010000885 DATCBB010000000
VERSION     DATCBB010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448704"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VII37_09735"
     CDS             complement(<1..1490)
                     /locus_tag="VII37_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5070062.1"
                     /translation="MAAILLVGIVSYTQLPVSALPEVDYPTIQVLTFYPGASPDVVAT
                     TVTAPLERQFGQLQGLSQMTSSSSGGTSVIVLQFALSLNIDVAEEEVQSAINASQNFL
                     PANLPSPPIYSKTNPADAPVLTLAATSNSIPLSQVEDLVDTRLAPKISQLSGVGLVTI
                     SGGQKPAVRIQANPAALSSYGINLEDLRTAVTQSSVNAAKGNFDGPRQDYQIDANDQL
                     VTSKDYRKVVVAYRDGAPVMLTDVANIVDGVENKNQAAWMNKTPAVIVNVQRQPGGNT
                     ISVVKRIKALLPQLEANLPKGIDITILTDLTTPIQASVSDVEFELMLTIALVVMVIFL
                     FLRNLYATIIPSVAVPLSLVGTFAAMYALGYSLDNLSLMALTISTGFVVDDAIVMVEN
                     ISRYIEAGEPPMQAALKGAEQIGFTILSLTVSLIAVLIPLLFMGDIVGRLFREFAVTL
                     AVTILVSAVVSLTLTPMMCARLLRHHKPEEQGWLYKMSERAFEAVIA"
     gene            complement(1426..>1724)
                     /locus_tag="VII37_09740"
     CDS             complement(1426..>1724)
                     /locus_tag="VII37_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070063.1"
                     /translation="VRATLRVVPEHVVLAEEVPLVQDLRRGTPVLQETRLRLEMRPAQ
                     GIPPLRETPIVRGARLIESVTSVYFEAGCDVAADGRHITGGHCELHAAAGFGAA"
BASE COUNT          397 a          524 c          449 g          354 t
ORIGIN      
        1 gcgatcaccg cttcaaacgc gcgctcggac atcttataca gccagccctg ctcttccggc
       61 ttgtgatggc gcagcaggcg cgcgcacatc attggcgtca aggtcagcga caccaccgcg
      121 gaaacaagaa tcgtcaccgc cagggtgacg gcgaactcgc ggaacaagcg gccgacgata
      181 tcgcccatga agagcagcgg gatcaacacc gcgatcaacg aaaccgtcaa agataaaatc
      241 gtgaacccaa tctgttccgc gcccttcaac gcagcctgca tgggtggctc gccagcctcg
      301 atatatcgcg aaatgttttc caccatgacg atggcgtcat ccacaacgaa tcccgtggag
      361 attgtcagcg ccatcagcga caaattatcc aagctgtagc ccagtgcgta catcgccgca
      421 aaagtgccca ccagcgacag cggcaccgcc acgctgggaa taatcgttgc gtaaagattc
      481 cgcaaaaata aaaaaatcac catgacgacc agcgcgatcg tcagcatcaa ttcgaattcc
      541 acatctgaca ccgaagcctg aataggcgta gtcaggtccg tgagtatagt gatatcgatc
      601 cctttcggaa gattcgcttc cagctgtggc agcagtgctt ttatccgctt caccacgctg
      661 atggtgtttc cgccgggctg ccgttgcacg ttcacgatga ccgccggcgt tttgttcatc
      721 cacgccgcct gattcttgtt ctccacgcca tctacaatgt tcgcgacgtc cgtgagcatc
      781 accggcgctc cgtcgcgata cgcgaccact accttcctat aatccttgct ggtgaccagc
      841 tgatcgttgg cgtcgatctg ataatcctga cgcggcccat caaaattccc ctttgccgca
      901 ttgacgctgg attgcgtaac cgccgtccgc aaatcctcca aattaattcc atacgaagac
      961 agcgccgccg gattagcttg aatgcgcacc gcgggcttct gaccaccgct gatggttacc
     1021 agcccaacgc cgctcaattg cgagattttc ggagccagac gtgtatcaac aaggtcttcc
     1081 acttgcgaaa gcggtatgga attcgaagta gccgccaaag tcagaaccgg agcgtccgcc
     1141 ggatttgtct tgctgtaaat gggcggagaa ggaagattcg cgggaagaaa gttttgcgaa
     1201 gcattaatcg ccgactgcac ttcttcttcc gccacatcaa tattcaggct cagtgcgaat
     1261 tgcaatacga tgacggaggt cccgcccgag ctgcttgacg tcatttggct cagtccctgg
     1321 agctggccaa actggcgctc taacggcgcg gtcaccgtag tcgcaactac atcagggctc
     1381 gcgcccggat aaaaggtaag tacttggatc gtggggtagt cgacttcagg cagcgccgaa
     1441 accggcagct gcgtgtaact cacaatgccc accagtaata tggcggccat cagcagcgac
     1501 gtcgcaaccg gcctcaaaat aaacggacgt gacggactca atgaggcgtg ctcccctgac
     1561 tattggtgtt tccctgagtg gagggattcc ctgggctggc cgcatttcca ggcgtagccg
     1621 cgtttcctgg agcaccggtg ttccccggcg tagatcctga accaggggca cttcttccgc
     1681 cagaaccacg tgttccggga ccacccggag cgttgcccgg acca
//
LOCUS       DATCBB010000886         1294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_254209, whole genome shotgun sequence.
ACCESSION   DATCBB010000886 DATCBB010000000
VERSION     DATCBB010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254209"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1294)
                     /locus_tag="VII37_09745"
     CDS             complement(<1..>1294)
                     /locus_tag="VII37_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5070064.1"
                     /translation="AAQPADYQPGAPPTFVLRYKTWVSRFSSDPSILNRTFTLNGEAR
                     TLVGIMPPRFAWGDADMWIPQTPVRGETSNGFPRFWFMLGRLKAGVTKDQGAADFAVL
                     AKQQATLHPPDYPTHFSVQLNSLADGVVGQFRATLFIVLAAVGLLLLIGCGNVANLLL
                     ARATTREKEFAVRAALGAGRRRLIQQLLVESMLLAIGGALLGSLLAWAGLKGLIAIIP
                     PNIVPAESVIRMNGRVLLFTLAVSMLTALIFGLAPALQASRRNLNDSLRDSSKGTSGG
                     SSHANLRNAVIVFEVALSLTLLIGAGLLMRSFAALHEVKLGLRPDHILVARLPLPQDR
                     YKTAPQVAGFFRPLLQRLNSLPGVVSDTETSTLPPYGGIPSDIEVPGKVHTEKWSSLF
                     QLCSEQYFTVLRIQFRRGRGFTESEVNNARKLAVINQTF"
BASE COUNT          296 a          357 c          390 g          251 t
ORIGIN      
        1 aaaagtttga ttgatgaccg ccagtttccg cgcgttgttg acttccgatt cggtgaaacc
       61 gcggccgcgc cggaactgta tgcgcaggac ggtgaaatat tgttcactgc aaagctggaa
      121 taacgaactc cacttctcag tgtgcacttt gccggggact tcaatgtccg atggaatgcc
      181 gccgtagggc ggaagcgtgc ttgtttcggt gtccgatacc acgcccggta gcgaattcag
      241 gcgctgaagg agcggtcgaa agaagccagc tacctgaggc gctgtcttat aacggtcctg
      301 cggcagcggc agccgcgcca ctagtatgtg atccggccgc agcccaagtt tcacttcgtg
      361 tagcgcggcg aaacttcgca tcaagagccc tgcgccgata agaagtgtga gcgaaagtgc
      421 tacttcgaaa acgattacgg cgttacgtaa attcgcgtgg ctggagccac cgctcgttcc
      481 tttactgctg tcgcgaagag aatcgtttaa gttgcggcgc gaagcttgca aagccggagc
      541 caaaccaaaa atcaatgcgg tgagcatgga cactgccagc gtgaagagca gcaccctgcc
      601 gttcatgcga atgacgcttt ctgcgggaac gatgttcggg ggaataatgg cgatcaatcc
      661 tttaagcccg gcccaggcga gcaacgagcc caataaggcg ccgccaatcg cgagcaacat
      721 gctttcgact agcaattgtt gaataaggcg gcgacgcccc gctccgagtg ccgcgcgaac
      781 cgcgaactcc ttttctcgtg tagtggcgcg cgccaaaagg agattggcga catttccgca
      841 accaatgagc agcagtagtc ccaccgcagc cagcacaatg aacaacgtcg cgcggaactg
      901 tccgaccacg ccatccgcta acgaattgag ctgcacggaa aaatgcgtgg gatagtccgg
      961 gggatgcaag gtggcttgct gcttggcgag tacggcgaag tcggcggcac cctggtcctt
     1021 ggtaacccca gctttcaggc gtcccaacat gaaccaaaac cgggggaatc cgttggaggt
     1081 ttcacctcgc acgggagtct gaggaatcca catgtccgca tcgccccagg caaagcgcgg
     1141 cggcatgatt ccgacaagtg tgcgggcctc gccattcagc gtgaaagttc tgtttaggat
     1201 cgagggatcc gagctgaaac ggctaaccca cgttttgtaa cgcagcacga aggtaggtgg
     1261 ggcgccaggc tggtagtctg cgggttgcgc cgcg
//
LOCUS       DATCBB010000887         1874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_384225, whole genome shotgun sequence.
ACCESSION   DATCBB010000887 DATCBB010000000
VERSION     DATCBB010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384225"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="VII37_09750"
     CDS             complement(<1..1859)
                     /locus_tag="VII37_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5070065.1"
                     /translation="MVGQCAAEKRRMLISDVPRNAVPVRSGLFEAVPKNVIVLPVLFE
                     DRVKAVIELASLSTFTASHLAFLEQLTASIGIVLNSIEATMQTEGLLKQSQQLATELQ
                     TQQKELQQTNEQLAQKAQQLAEQNVEVERKNQEIEQARRALEEKAKELALTSKYKSEF
                     LANMSHELRTPLNSILVLGQQLSDNPDSNLTPKQVEFARTIHGAGTDLLNLISDILDL
                     SKIESGTVSVEAEEVFFASLLEMIGRPFKHEAENRRLSFEVAADPHLTRSLVTDSKRL
                     QQVLKNLLSNAFKFTEHGGVRLSVATVESGWSENHPILDGAASVIAFEVSDTGIGIPT
                     EKQRIIFEAFQQADAGTSRKYGGTGLGLAISRELASLLGGEIQLRSQPGKGSTFTLYL
                     PKTYVGPSNTLGTIKERRSPSSTTAMQLAASTLEHPTETIPDDRESLEPGDNVLLIVE
                     DDPHYARVLADLSRDKGFKVLVASRGAEALALAREYHPTAVSLDVFLPDMLGWTVLNH
                     LKQDPATRHIPVQMLTMDEDRHHGLARGAFSFVTKPTTPEGLESALEKIKTYSAPRRR
                     RLLVVEDNPAEQLSIRELLTHSDIDVTVAETGTEALMSVNENNFDCVVLDLRLP"
BASE COUNT          376 a          548 c          484 g          466 t
ORIGIN      
        1 ggcaaccgca ggtccagcac gacgcaatca aaattatttt cattcacgct catcaacgct
       61 tccgtaccgg tttccgctac ggtcacgtca atgtccgagt gcgtaagcaa ttcgcgaata
      121 ctcaattgct ccgcgggatt gtcttccacc accagcaagc ggcgccggcg tggagcagaa
      181 tacgttttga ttttttccag cgctgactcc aatccttcag gcgtcgtcgg cttggtcaca
      241 aaagaaaacg ctccccgagc caatccgtga tgacgatctt catccatggt cagcatttgc
      301 accggaatgt gccgcgtggc gggatcctgt ttcaagtgat tcagcaccgt ccagcccaac
      361 atatccggta gaaaaacatc cagcgataca gcggtgggat ggtactcgcg cgcgagcgcc
      421 agtgcttcgg cgccgcgcga ggccaccagc accttgaatc ctttgtcgcg ggatagatct
      481 gccaggacgc gcgcgtaatg cggatcgtct tccacgatca gcagcacatt atcgccgggc
      541 tcgagactct cgcgatcgtc cggaatcgtc tctgtcgggt gctccagggt tgaagccgcg
      601 agctgcatgg cggtcgtgct cgacggcgat ctgcgctctt tgatcgtgcc cagcgtgttc
      661 gacggtccaa cataagtctt cggcaaatac agcgtaaacg tgctgccttt gccgggctgg
      721 cttcgcagtt gaatttctcc tcccagcaaa ctggccagtt ctcggctaat ggccaggccc
      781 aatccggttc cgccgtattt acggcttgtt cccgcatcag cttgttggaa ggcctcaaaa
      841 ataattcttt gcttttccgt gggaatgccg ataccggtat ccgaaacttc aaaggcgatt
      901 actgacgcgg cgccatctaa aatcggatga ttctcactcc atccgctttc cacggtggca
      961 actgacaatc gcaccccgcc gtgttcggtg aacttaaatg cgttggatag taagtttttc
     1021 aacacctgct gcagtcgctt cgagtccgta acgagactgc gcgttagatg cgggtcggca
     1081 gccacttcaa acgacaatct gcgattctcc gcttcgtgtt tgaaaggccg cccgatcatt
     1141 tccagcaggc tcgcgaaaaa tacttcttcc gcttccaccg acaccgtgcc ggattcgatc
     1201 ttcgacaggt ccagaatgtc gctgatcaaa ttcaacaagt ccgtgcccgc gccgtgaatg
     1261 gtgcgtgcga actccacttg cttgggcgtc aaattgctgt cggggttgtc gctcaactgc
     1321 tggcccagaa ctaggatgct gttcagcggc gtgcgcagtt cgtgagacat attggccagg
     1381 aattccgact tgtacttgga cgtgagggcc agctccttgg ctttttcttc cagtgcgcga
     1441 cgcgcttgct cgatttcctg gtttttgcgt tctacttcaa cgttctgttc cgcgagctgc
     1501 tgagctttct gcgccaattg ctcattggtc tgctgcaatt ccttctgctg cgtttgcagt
     1561 tcagttgcta attgctgaga ttgtttcagc aagccttccg tctgcatggt ggcttcaata
     1621 ctgttcagca cgatgccgat gctggctgtt agttgttcga ggaaggccag gtgcgacgcc
     1681 gtaaaggtgc tgagcgaggc caattcaatg accgctttaa ctcggtcttc gaacaaaacc
     1741 ggcagcacaa tcacattttt tggcactgct tcgaataagc cggatcgcac cggcacggca
     1801 ttcctgggta cgtccgaaat caacatacgc cgcttctccg cggcgcactg tccaaccaat
     1861 ccttcgccaa cccg
//
LOCUS       DATCBB010000888         1541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_448774, whole genome shotgun sequence.
ACCESSION   DATCBB010000888 DATCBB010000000
VERSION     DATCBB010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448774"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VII37_09755"
     CDS             <1..451
                     /locus_tag="VII37_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY5070066.1"
                     /translation="ELIENVKVGSGGDAVPIHAVTDVTGFGVLGHAREMALGDPSRGI
                     ASTSFELDYSAFNYLPGAVDAARAGHLSGGLKNNRAFVADCAAFAENVPQQFQDLLFD
                     PQTSGGLLVAIASDFAADCIAVLSGHGVEARQIGRVVAKRTSLIFVR"
     gene            518..>1541
                     /locus_tag="VII37_09760"
     CDS             518..>1541
                     /locus_tag="VII37_09760"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY5070067.1"
                     /translation="MDTITHGIAGALIGKALFNGDDLFAMRAMSRRRIVAWSATLGGI
                     FPDADTFRDMLSHNELLVITWHRSTTHSLLCLPIWALLLAALTRWIVRWRKWDAPSFV
                     ALTGIYAVGILSHILMDLITSFGTMVWSPLDWARPAWDLVFIVDFTFTGILLVPQILA
                     WVHERPEKSWRRALVSLAIFILAIFSVQAIGNAVGAPISASTVFLFDVMLAALFLIPL
                     LNNKKSLSRTQKRAQRSDFEEGNSVSENLSANVQTNLYRRWNRAGLICGCGYIAVAVF
                     AHHAALGRVKNFASLQKLEGTSMGALPFPPSLWRWDGLVRTPRGVYELRMDLSEKSAF
                     EAAANPA"
BASE COUNT          291 a          405 c          465 g          380 t
ORIGIN      
        1 ggagctgatt gaaaatgtaa aggtaggcag cggcggagac gcagttccga ttcacgccgt
       61 aaccgatgtg accggcttcg gcgtcctggg acacgcgcgc gaaatggcgc tgggcgatcc
      121 gtcccgcggc atcgcgtcaa catcatttga gctggattac tccgcgttca actatcttcc
      181 cggagctgtc gacgccgcgc gtgccggtca tctctcagga ggactaaaaa acaaccgcgc
      241 cttcgtagct gattgcgcgg cattcgcgga aaatgtcccg cagcaatttc aggacttact
      301 cttcgatccg caaacgtccg gcggcctgct cgtcgccatc gcttccgatt ttgccgccga
      361 ttgcatcgca gttctttcgg gtcacggcgt cgaagcccgc cagatcggcc gcgtcgtcgc
      421 caagcgcacg tcgctcatct ttgttaggta gagagattag gcaatgaatg atcagcgtga
      481 attgattcac tgaataaata gtccgcaata aataatcatg gacaccatca cacacggaat
      541 cgccggagcc ttgatcggca aagcgctgtt taatggcgac gatttgttcg cgatgcgggc
      601 aatgagccgg cggcgcatcg tggcatggtc ggcgacgctg ggagggattt ttccggacgc
      661 cgatactttc cgcgacatgc tgtcgcacaa cgaattgttg gtgatcacct ggcatcgcag
      721 caccacgcat tcattgcttt gcttgccgat ttgggcgttg ctgctcgccg cgctcactcg
      781 ctggattgtg cgctggagaa aatgggacgc gccatcgttt gtcgcgctca cgggaattta
      841 cgccgtggga attctcagcc acattttgat ggacctgatc accagcttcg gcacgatggt
      901 ttggtcgccg ctcgattggg cgcggccggc gtgggattta gttttcatcg tggatttcac
      961 gttcacggga attttgctgg tgccacagat tctggcgtgg gtgcatgagc gtccggaaaa
     1021 aagctggcgc cgcgcgctgg tgtcgctggc gatttttatt ctggccattt tctcggtgca
     1081 ggcaatcggc aacgctgtcg gcgcgcctat ttccgcttcg actgtttttc ttttcgacgt
     1141 aatgctcgcg gcgctgtttt taattccgct gctcaacaat aaaaaatctt tgtcgcgcac
     1201 gcagaaaaga gcgcagcgtt ctgattttga ggaggggaat tctgtttcag aaaatctgtc
     1261 ggcaaatgtg cagacaaatt tgtatcggcg gtggaatcgc gcgggcctga tttgtggttg
     1321 cgggtatata gcggtggcgg tgtttgcgca tcacgctgcg cttggtcgcg taaagaactt
     1381 tgcgtcactc cagaagctcg aaggtacgtc gatgggggcc ctgccgtttc ctccttcgct
     1441 gtggcgctgg gatggattgg tgcgcacgcc gcgcggcgtt tacgaattgc ggatggatct
     1501 gagcgagaag tcggcgtttg aagctgcggc gaatcctgcg c
//
LOCUS       DATCBB010000889         1569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_384339, whole genome shotgun sequence.
ACCESSION   DATCBB010000889 DATCBB010000000
VERSION     DATCBB010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384339"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VII37_09765"
     CDS             <1..262
                     /locus_tag="VII37_09765"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070068.1"
                     /translation="NLEKAIRWSRQLSEYFSPHNGKGVRILGPATAPLARLKKEYRFQ
                     FLLKSPKRSPLTKLLAGALDQCEANEIPDTAVIVDMDPLSLM"
     gene            complement(270..>1569)
                     /locus_tag="VII37_09770"
     CDS             complement(270..>1569)
                     /locus_tag="VII37_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5070069.1"
                     /translation="LLLFIGSVIGSGIFLTPSLILQQLDGSVGLSLLVWFGGGILSLL
                     GALTYAELAASNPEAGGLYCYIRDGFGRATAFLYGWSLFLIIASGTIAALARAFTRYL
                     AEIIPLSPIASTVIGVAMIAAVTAVNIWGTRKSSDLQNWTTLTKVGMVAILSAVLLSM
                     GHHAADLPHALGTSQRGFSLASNFGLAMIAVLWAYEGWQFGTYSAGEVLKPQQAFPKA
                     FLMGSLFLMALYLIAVIAYLVALGPAAATVSDAIAAQAMSLVLGPWAGKIVAIAILIS
                     TFSATNSVILTAPRVFYAMANDNLFFKRLAQVHPRFQTPAWAIIALGVWSAILSCAGK
                     FAELASGAIFIGWIFYGLGAAAIFPIRRASRGLPIPYRVPGYPWTPLVFVIVAAAIVG
                     NAIFLAFVDPLQFHYLAVAIVIFLLGLPAYYFWRRKQTSA"
BASE COUNT          416 a          395 c          475 g          283 t
ORIGIN      
        1 gaatctggag aaagcaattc gctggtcgcg ccaactctcg gaatattttt caccacacaa
       61 tggtaaaggc gtacggattc tgggtccggc caccgcacct ttggcgcggc tgaaaaagga
      121 ataccgcttt caatttctgc tgaaatcgcc aaagcgctcg ccgctgacca aactcctggc
      181 cggggcactc gatcagtgcg aggctaacga aatcccggat acggcagtga ttgtggacat
      241 ggacccgtta agcctgatgt aacgatgggc taggcggaag tttgtttcct gcgccagaaa
      301 taataggccg ggagtccaag caaaaaaatt acgatagcta cagccaaata gtggaactgg
      361 agcggatcga cgaaggcgag aaagatggcg ttgccgacga tggccgcggc gacgattacg
      421 aaaactagcg gtgtccaggg atagcccggt acgcggtaag gaattggcag gcctcttgaa
      481 gcgcgacgaa tcgggaaaat tgccgcagcg cctagcccgt agaaaatcca tccgataaaa
      541 attgcgccgg aagccagctc agcgaatttg cccgcgcaag aaagaatcgc ggaccataca
      601 ccaagcgcga tgattgccca ggctggcgtt tgaaaacgcg ggtgcacctg cgcaagtctt
      661 ttgaaaaaga gattgtcgtt ggccatggcg taaaaaacgc gcggcgccgt aaggatcacg
      721 ctattggtgg cgctaaaggt ggagatcaaa atggcgatgg ctacgatttt tccagcccat
      781 ggccctagca cgaggctcat ggcttgggct gcgatggcat cgctcacggt ggctgcagcg
      841 gggccgagcg cgaccagata agcgattacg gctatcaagt aaagagccat taaaaatagg
      901 gagcccatca ggaacgcttt ggggaaagcc tgctgcggct tcaaaacttc tccggcactg
      961 taggtgccga attgccatcc ttcataagcc cacagcacgg caatcatcgc gagaccaaaa
     1021 tttgaggcca gcgaaaatcc gcgttgtgaa gtgccaagag catggggcag atcggcagca
     1081 tggtgaccca tggagagcag cacggcgctg agaatcgcga ccatgccaac tttcgtcaag
     1141 gtggtccaat tttgcagatc ggaacttttg cgtgtgcccc agatgtttac cgcggtgacc
     1201 gccgcgatca tggccacgcc gatgacggta cttgcgatag gcgagagcgg gataatttcc
     1261 gccaggtagc gggtgaaagc gcgtgccagc gcggcaatgg tgccgctggc gattatcaag
     1321 aaaagactcc agccataaag aaaggcggtg gcgcggccga aaccatcacg aatgtagcag
     1381 tacagtccgc cggcttccgg attggaggcg gcgagttcgg cgtaggttaa cgcaccgagg
     1441 agcgaaagga ttccgccgcc gaaccagacc agtagggata atccaactga accatcaagc
     1501 tgctggagga ttagactggg agtgagaaaa attcccgagc cgattacgga gccgatgaag
     1561 agaagtaag
//
LOCUS       DATCBB010000890         1251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_189700, whole genome shotgun sequence.
ACCESSION   DATCBB010000890 DATCBB010000000
VERSION     DATCBB010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189700"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="ribB"
                     /locus_tag="VII37_09775"
     CDS             complement(<1..719)
                     /gene="ribB"
                     /locus_tag="VII37_09775"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY5070070.1"
                     /translation="MTGISPVQEIIEEIRAGRMVVLIDEEDRENEGDLVFAAEFVTPE
                     KINFMAKHGRGLICLTLTEAHCKQLNLPLMVRENGLSLATNFTLSIEASNGVTTGISA
                     ADRARTVQAAAARNAKPADIVQPGHIFPLRAQNGGVLVRAGHTEAGCDLAQLAGLAPA
                     SVICEILKDDGEMARLPDLIVYAKQHGLKIGTIADLIEHRSQHEKLVERVARRKVQTA
                     YGEFELVTYVDKTTQRTHLALV"
     gene            complement(716..>1251)
                     /locus_tag="VII37_09780"
     CDS             complement(716..>1251)
                     /locus_tag="VII37_09780"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY5070071.1"
                     /translation="LRLVIATSALDLSDVQTGDSIAVNGICLTVVEHGAGSFAVDVSR
                     ETLNCTEGLDAIGAPVNLEKALRLADRLGGHMVSGHVDGVGEVIEFSNLGESWKLVIS
                     PPQTLAKYIAIKGSITINGVSLTINQVSGGEFSVNLIPHTLVMTNLKNLRAGSRINLE
                     VDLIARYVERMMQRDIA"
BASE COUNT          248 a          386 c          328 g          289 t
ORIGIN      
        1 accagggcca ggtgagtgcg ctgcgtggtc ttgtccacgt aggtcactaa ttcgaattcg
       61 ccgtaagcag tttgcacctt gcgccgcgcc acgcgctcga ccagtttctc gtgctggctg
      121 cggtgctcga tcaggtcggc aatcgtgccg atcttgagac catgctgttt ggcatacacg
      181 atcaaatcgg gcaggcgtgc catttcgccg tcgtccttga gtatctcgca gataaccgaa
      241 gccggagcca gtcccgccag ttgcgccaaa tcgcagcccg cctcggtatg cccggcgcgc
      301 accagcacac cgccattttg cgcgcgcagc ggaaagatat ggccgggctg cacgatgtcg
      361 gcaggcttgg cattcctggc cgccgcggct tgcacggtgc gcgcacgatc ggctgccgag
      421 atgccggtag tcactccgtt cgaggcttca atcgacagcg taaaattagt cgccaacgac
      481 aaaccgttct cgcgcaccat cagcggcaga ttgagttgct tgcagtgagc ctcagtcaag
      541 gtcagacaaa tcagcccgcg gccatgcttg gccatgaagt tgattttttc cggcgtgacg
      601 aattcggcgg cgaataccag atccccttca ttttcgcggt cttcttcatc aatcagaacg
      661 accatgcgtc cggcgcgaat ttcttcgatg atttcctgca cgggtgaaat tcctgtcatg
      721 caatatccct ttgcatcatc cgttctacat aacgcgcgat caagtcgact tcgagattga
      781 tccggctgcc cgcgcgcaaa tttttcaggt tagtcatcac cagtgtgtgc ggaatcagat
      841 tgacgctgaa ctcgccgccg ctcacctggt tgatggtgag gctgacaccg ttgatggtga
      901 tcgagccttt gatcgcaatg tatttcgcca atgtttgcgg tgggcttatt accagtttcc
      961 agctttcgcc cagattggaa aattcgatga cttcgcccac gccatcgaca tggccgctga
     1021 ccatgtgtcc gcccagcctg tcggccaagc gcaatgcctt ttccagattt accggcgcac
     1081 cgattgcatc caacccctcg gtgcaattca acgtttctcg cgaaacatcc acagcgaaac
     1141 tgccggcgcc atgttcgact acagtcaggc atatcccgtt caccgcgata ctgtcgccgg
     1201 tctgcacatc gcttaaatcc agagcgctgg tcgcgatcac cagccgcaac c
//
LOCUS       DATCBB010000891         1055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_352434, whole genome shotgun sequence.
ACCESSION   DATCBB010000891 DATCBB010000000
VERSION     DATCBB010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352434"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..929
                     /locus_tag="VII37_09785"
     CDS             543..929
                     /locus_tag="VII37_09785"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEY5070072.1"
                     /translation="MLIGQSHFKYAVLCAAIALSAAGCATTPIPNEKIAVAKSSVQRA
                     EQAGAPELAPVEMAAARDKLTRAEKAAADREAQPATQLAEQANADAQLAEASAQEQRS
                     HKAAMEFDANLQALRQETLRSSQSNQ"
BASE COUNT          263 a          304 c          293 g          195 t
ORIGIN      
        1 aacagaagcc gcggatcatc gtgctcacca actacgactt gcccgaatat aagaatgcag
       61 cgatcgcact cggcgcaacg cattttctcg acaaggcccg cgactacggc cgcctgccgg
      121 aagtactgca cgaaatctgc gaagcgcatc tttcgaaaca ctgagtgcgc gtaccgcatt
      181 ctgtcaataa aactttaagc aagtgaaaaa caccatattt ttcgggaatt tctgccattc
      241 gagctgtcat ctctctccga cattctcgta atgtctacca tctcgtgcgg aaatatctac
      301 accgaataat ctcgaaagtc cgcgcgccgt gcgaaatgcg cgcggcgccc gcgcggcgcc
      361 cgatattgcg cttggcacgc attttgctcc ttaacataag gtcgagagaa ttttttagga
      421 gacgaaatga ggcaaatatc cttttccaag gcgaaccccg aactttaaaa acatagcggc
      481 acccgtggac tcctgtgatt tggtccgccg gatttggccg cgacatcacg aggagtacac
      541 acatgttgat aggacaatct catttcaagt acgcggttct gtgcgcggca atcgcattga
      601 gcgccgcggg ctgcgccact acgccgatcc caaacgagaa aattgcggtg gcaaagtctt
      661 ccgtgcagcg cgccgagcaa gcgggcgcgc cggaattggc gccggttgaa atggccgcgg
      721 cacgcgacaa attgacgcgc gctgaaaagg cggcggctga ccgtgaagcc cagcccgcga
      781 cccaactggc ggagcaggcg aacgcggacg cgcagctggc cgaggcgagt gcgcaagaac
      841 agcgctcgca caaggcggct atggaattcg atgcaaacct gcaagcgctg cgccaagaaa
      901 ccttgcgcag cagccaatcc aatcaatgag gatgtaacat gaacgcaaaa ttaatgaccg
      961 gaatggggtt ggctctggcc ttaggtgcct gcgtctcgat gccgcaacct aacgcagcac
     1021 tcgagaatgc gcgcgcggcg gtgcagacgg ccgag
//
LOCUS       DATCBB010000892         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_287303, whole genome shotgun sequence.
ACCESSION   DATCBB010000892 DATCBB010000000
VERSION     DATCBB010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287303"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VII37_09790"
     CDS             <1..591
                     /locus_tag="VII37_09790"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY5070073.1"
                     /translation="IIMETLGSRRGEMLKMSNHGSGRVRMDFKIPSRGLIGLRSQLLT
                     DTRGTALIHSIFEGWTDYAGEMALRPTGALVADRSGPSTAFALWNIQERGELFIGPAI
                     DVYEGMIVGENSREQDMDVNVTKEKKQTNMRSSSADEAIRLIPHRELSLEQAIEFIAD
                     DEFVEVTPKSIRLRKKVLQANRRPRRWQEIRASQTS"
     gene            846..1937
                     /locus_tag="VII37_09795"
     CDS             846..1937
                     /locus_tag="VII37_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY5070074.1"
                     /translation="MNLSNAAEQRLSSENCTIAATATALPPHKITREDVKYYMGRVFD
                     IPERKLEAMMSIVDNARVHNRHAIFPIEYTVEPRPLSKTNEEYIEHAIKLGREAAEKC
                     LKRAGLAPTDIDLIITVSCTGFMIPSLDAHLIHLMGFRPNVRRMPFTELGCAAGAMAL
                     GRAADYLKAEPGGNVLIIAVELPSLTFQRKDISQANLISSILFGDGAAAVVVRGKPVH
                     GPKILFSETYTFPDSLGAMGFDLRDSGFHILLAKDVPEMIGAKINELVDGFLERCGKK
                     REDIKGWILHPGGARLLGNVETQLGLTQCDTQPSWDVLANVGNLSSATILFILQEWLE
                     KRPLKTGEYALLAAFGPGFSAEFLLLQWT"
     gene            1928..2500
                     /locus_tag="VII37_09800"
     CDS             1928..2500
                     /locus_tag="VII37_09800"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY5070075.1"
                     /translation="MDVTVIAFLALLVAVAALRIVELQISRRHQASMIERGANKVAEP
                     RFGWMVALHTAVLVGAGVEVVFLHRPFIPVLAALMFTVFLAANAVRWWVIRTLGEHWN
                     VQVMDSTRLGVIVTGPFRFVRHPNYAAVFAEMLALPLIHTAWITALAGSLAHVVVLAQ
                     RLSTEERVLFADPDYRSAMAGKPRFLPGLF"
BASE COUNT          573 a          671 c          709 g          575 t
ORIGIN      
        1 atcatcatgg aaactctggg cagcaggcgc ggtgaaatgt tgaagatgag caatcacggc
       61 tccgggcgcg tgcgcatgga tttcaaaatt ccttcgcggg gcttgattgg attgcgcagc
      121 cagttgttga cggacacacg cggaaccgcg ctgattcatt ccatcttcga aggctggacg
      181 gattatgccg gcgaaatggc actgcggcca accggcgcac tggtcgccga tcgcagcggt
      241 ccttccacgg cgtttgcgct ttggaatatt caggaacgcg gggagctgtt catcggccct
      301 gcgatcgatg tatacgaagg catgattgtg ggcgagaatt cgcgcgaaca ggacatggac
      361 gtaaacgtca ccaaagaaaa gaagcagacc aacatgcgct cttccagcgc cgacgaagcc
      421 atccgtttga tcccgcaccg cgagctgagt ctggagcagg ctattgaatt tatcgccgat
      481 gatgaattcg tggaagtgac ccccaagtcg atccgtctac gcaaaaaagt tttgcaggca
      541 aatcggcgcc cgcgccggtg gcaggaaatt cgcgccagcc agacgagcta gccgcacaaa
      601 aaaatacaaa gcgctaagcc cataaataaa ttgcaagaag tttacatgtg cgtctcggtc
      661 taaatccgga caccgcaacc aaacacccag ttccgagtgt tttgactact cattttggcc
      721 tggaatgatg taaagtctac cgcttgcacc caaccatgct ggaaaacgta gcatcttagt
      781 ttggcgtcca aaccgtggct cgattcgtca taatccgtgc cacaatatga tctacagggg
      841 attcgttgaa cctttcaaac gctgcagagc aacgcctgag ttctgaaaat tgcaccattg
      901 ccgcaactgc gacagcactc ccaccccaca aaatcacccg cgaggacgtg aaatattaca
      961 tgggccgggt gtttgacatt cccgagcgca agctcgaagc catgatgagc attgtggaca
     1021 acgcacgcgt tcataatcgt cacgccattt tccctatcga atacaccgtc gaaccgcggc
     1081 ccctgtcgaa gaccaacgaa gagtacatcg agcacgcgat caaattgggt cgtgaggcgg
     1141 cggagaaatg cctgaagcgc gccggacttg cgcctaccga catagatctc atcatcaccg
     1201 tgtcgtgtac cggattcatg attccctctc tcgatgcaca tctcatccac ttgatgggtt
     1261 ttcgtccgaa tgtgcgacgc atgccattta cggaactggg ctgcgccgcc ggggccatgg
     1321 cattggggcg cgcagcggat tacttaaaag cagagcccgg tggcaatgtg ttgatcatcg
     1381 cggtcgaatt gccttcactg acgtttcagc gcaaggatat ttcccaagct aaccttattt
     1441 catccatttt atttggcgac ggcgcggctg ccgtagtcgt acgtggaaag cccgtgcatg
     1501 ggccgaagat tttattttcg gaaacctata cctttccaga ctcgctgggc gcgatgggat
     1561 ttgacctgcg ggattcggga tttcacattc tgttggccaa ggatgtaccg gaaatgattg
     1621 gcgctaaaat caacgaactg gtcgacgggt tcctggagcg ctgcgggaaa aagcgcgagg
     1681 acatcaaagg atggattctg catcctggcg gcgcgcgctt gttgggcaat gtggaaacac
     1741 aactcggtct gacacaatgt gatactcaac cgtcgtggga cgttcttgcg aacgttggga
     1801 atctctcgag cgctacgatt ctgtttattc ttcaggaatg gctggaaaag cgcccgctaa
     1861 agaccgggga atacgcgttg ctcgcggctt ttggcccggg cttcagcgcc gaatttttgt
     1921 tactgcaatg gacgtaactg ttatcgcatt tctcgcactg ctggtggccg tggcggcgtt
     1981 gcgaatcgtc gaactgcaaa tttcacggcg ccatcaagca agcatgatcg agcgcggcgc
     2041 caacaaagtt gcggagccac gttttggctg gatggtcgcg ctgcacactg cggtgctcgt
     2101 gggcgcgggc gtggaagtgg tttttctgca ccggccattt attcctgtgc tagccgcgct
     2161 aatgttcacc gtttttctgg cggccaacgc ggtgcgctgg tgggtaattc gcacgctggg
     2221 cgaacattgg aacgttcaag tcatggactc cactcgcctg ggcgtaattg ttactggacc
     2281 tttccgcttc gtacggcatc caaattacgc agcggtgttt gcggagatgc tggcgctacc
     2341 tctgatacac acggcgtgga tcactgcgtt ggccggttcg ctggcgcatg ttgtggtgct
     2401 ggcgcaaaga ctttcgaccg aagagcgcgt actgtttgcc gatccagatt accggtcggc
     2461 catggcggga aaaccgagat tcctgccggg cctgttctaa atacatacta aagactcgat
     2521 gatgagcc
//
LOCUS       DATCBB010000893         1020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_223806, whole genome shotgun sequence.
ACCESSION   DATCBB010000893 DATCBB010000000
VERSION     DATCBB010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223806"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..>1020)
                     /gene="aroE"
                     /locus_tag="VII37_09805"
     CDS             complement(205..>1020)
                     /gene="aroE"
                     /locus_tag="VII37_09805"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY5070076.1"
                     /translation="VAGDPVAHSLSPAIMNAALHRENVNGVYVALHAKTLKDLLTCVR
                     EIPIHGLSITMPYKQTILSHLDNTDSQTTKIGACNTLVRAHDGKLYGFNTDAVGVVRP
                     LEQRMTLEKAKILVLGAGGAARAAVFGLKERGAEVYILNRSAIAAQKLARQARARTIK
                     RPDLKKVDFDVIINATPVGMTNTRESPLNAEEIKARYLFEMIYDPPETQLVKLAMERG
                     VEVIPGTEMFVHQAARQFEIWTGKPAPWDDMLKVVGVALRQRQAANGTKMPKK"
BASE COUNT          213 a          268 c          300 g          239 t
ORIGIN      
        1 tcatgaagag aatctcaaag cagctcgaac tggctcccgg cggatatatt tcagtgctgg
       61 acccagggaa agggtggacg caactttcag cggaaagtgt gggctgtgct ccgataccat
      121 cagggagcag gcgatcccgc agagctatgg ataaatccga gtggaggcgg agcgttcgat
      181 agtagaccga gtgccggttg aagactactt ctttggcatt ttggtgccat tcgccgcctg
      241 gcgctgccgc agtgccacgc cgacaacttt gagcatgtcg tcccaggggg cgggctttcc
      301 cgtccatatt tcaaattgac gcgcggcctg gtgcacgaac atctcagttc cggggatgac
      361 ttcaactcca cgctccattg ccagtttcac aagctgagtt tccggcgggt cgtaaatcat
      421 ctcgaaaaga tagcgtgctt taatttcttc ggcattcaac ggagattcgc gggtatttgt
      481 catgcccacg ggggtggcat tgatgatgac atcaaaatcc actttcttga ggtcggggcg
      541 tttgatcgtg cgcgcgcggg cttggcgggc tagcttctgt gcggcaatcg cgcttcgatt
      601 gagaatgtag acttctgcgc cgcgctcctt caatccgaat acggctgcgc gggcggcgcc
      661 tccagcgccc agcacgagaa tttttgcttt ctccagtgtc atccgctgct ccagcgggcg
      721 aaccacgccg actgcgtccg tattgaaacc gtatagtttg ccgtcatgcg ctcgaaccaa
      781 cgtattgcac gcaccaatct tggttgtctg ggaatcggtg ttgtcgagat gggagaggat
      841 ggtctgcttg tagggcatgg taatcgacag cccatgaatc ggaatttcgc ggacgcaggt
      901 cagcagatcc ttcaaagtct tggcgtgcaa cgcgacgtac actccgttta cgttctcgcg
      961 atgcaaagcg gcattcatga tggcaggaga taaagaatgt gcaacagggt cgcccgcaac
//
LOCUS       DATCBB010000894         1064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_125673, whole genome shotgun sequence.
ACCESSION   DATCBB010000894 DATCBB010000000
VERSION     DATCBB010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125673"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1064
                     /locus_tag="VII37_09810"
     CDS             <1..>1064
                     /locus_tag="VII37_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5070077.1"
                     /translation="LNVDRNINTRVKRQMERAQKEYYLNEKLKAIQKELGRGEKSEID
                     ELKKKIDTAGMSKEVHEKAIQELKRLELMPPMSAESTVSRNYLDWLLAVPWKKKSKEI
                     RDILAAEKILNEEHYGLEKIKDRILEFLAVRQLVKNPKGSILCFVGPPGVGKTSLAMS
                     IGHATGRKFVRVSLGGVRDEAEIRGHRRTYIGALPGQIIQMMKKAGTVNPIFVLDEVD
                     KMSTDFRGDPSAALMEVLDPELNHAFTDHYLDVEYDLSKVMFVCTANVLHTIPQPLQD
                     RMEILRIPGYTEQEKLQIAKRFLVKRQREATGLQESNLSFTDEGLLHIIRHYTQEAGV
                     RNLEREIQNISRKMARKVVT"
BASE COUNT          275 a          333 c          268 g          188 t
ORIGIN      
        1 gctcaacgtc gaccgcaaca tcaacacccg cgtgaagcgc cagatggagc gcgcgcaaaa
       61 agagtattac ctcaatgaaa agctcaaagc cattcagaag gaactgggcc gcggcgaaaa
      121 aagcgaaatt gacgagctga aaaagaaaat cgataccgct ggcatgtcca aggaagtaca
      181 cgaaaaggct attcaggagc tgaagcgcct ggaattgatg ccgcccatgt ccgcggagtc
      241 caccgtcagc cgcaattatc tcgattggct gctcgctgtg ccctggaaga aaaaatctaa
      301 ggaaattcgc gacatcctgg ccgccgaaaa aatcctcaac gaagagcact acggcctgga
      361 aaagattaaa gaccgcatcc tcgaatttct ggccgttcgc cagttggtga aaaaccccaa
      421 gggctccatc ctgtgcttcg tcgggcctcc cggcgtcggc aaaacctccc tggccatgtc
      481 catcggccac gccaccggcc gcaagtttgt tcgtgtctcg cttggcggcg tgcgcgacga
      541 ggctgaaatt cgcggccacc gccgcaccta tatcggcgcg ctgcccggcc agatcatcca
      601 gatgatgaaa aaggccggca ccgtcaatcc cattttcgtt ctcgatgaag tggacaaaat
      661 gtccaccgac ttccggggcg atccatccgc agcgctgatg gaagtcctcg accccgaact
      721 gaatcacgct ttcaccgacc actacctcga tgtcgaatac gatctttcga aagtaatgtt
      781 cgtttgcacc gccaacgtcc tgcacaccat tccgcagcct ttgcaggacc gcatggaaat
      841 cctgcgcatc cccggctaca ccgagcagga aaaactccag attgccaagc gcttcctggt
      901 caagcgccag cgcgaagcca ccggcctgca ggaaagcaat ctatcgttca ccgacgaagg
      961 cctgctgcac atcatccggc actacaccca ggaagcaggc gtccgcaatc tcgagcgcga
     1021 aattcagaat atctcccgca agatggcccg caaggtggtc accg
//
LOCUS       DATCBB010000895         1138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_417911, whole genome shotgun sequence.
ACCESSION   DATCBB010000895 DATCBB010000000
VERSION     DATCBB010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417911"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VII37_09815"
     CDS             <1..1026
                     /locus_tag="VII37_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5070078.1"
                     /translation="KKFRDEMMKIDLRKRLVELDAQPLTDYTAGYAPEVQQWWDGYGP
                     SNWGATSEDTSAFVALASLQGMASGDDSKRVILPGGLGCITHKLVEVLQPKYKERMLG
                     EATVVAVVPGDDNVRITYFHDGQLTTVAAKAVIMCTQKHITSRLVMGIPYDQKTAMRR
                     TRYAPYPVVNAIFDKPVYNRGYDTWCPGNSFTDFIVADWTIRNTPGYHQKHNILSFYT
                     PLRETQRSTLLEEDNCKKLAAQVLADFQKLLPEFNVDPVEVRIYRRGHPMFMGVPGQF
                     TKNRIAAAHPMDRIYFSGNDSGGPESLTSEAIRFSQGGAEWAEMILTGKPGAKDFAEK
                     VLNAREF"
BASE COUNT          262 a          329 c          303 g          244 t
ORIGIN      
        1 aaaaaattcc gcgacgagat gatgaaaatt gatctccgca agcgcctcgt cgagctagac
       61 gcgcaacctc tcaccgacta caccgcgggc tacgccccgg aagttcagca atggtgggac
      121 ggctacggcc cttccaactg gggcgccacc agcgaagata cttccgcatt cgtcgcgctc
      181 gcttcgctgc agggcatggc ttcaggcgac gacagcaagc gcgtcattct tcccggcgga
      241 ctcggctgca tcactcacaa gctcgtggaa gttctgcaac ccaaatacaa agagcgcatg
      301 ctcggcgaag ccacggtggt ggccgttgtt cccggtgacg acaatgtgcg cataacgtat
      361 ttccatgatg gacagttgac caccgtagcg gcgaaagcgg tgatcatgtg cacgcaaaaa
      421 cacattacgt cgcgactggt gatgggcatt ccttacgatc agaagacggc gatgcggcgc
      481 acgcgctacg caccttatcc ggtggtgaac gccatcttcg ataagcccgt ttacaatcgc
      541 ggctacgaca cctggtgccc cgggaattcg ttcaccgatt ttattgtggc ggactggacg
      601 attcgcaata ctcccggcta tcaccagaaa cacaacattc tttcgttcta cacgccgctg
      661 cgcgagactc agcggtccac gcttctcgaa gaagacaatt gtaagaagtt ggccgcgcaa
      721 gtgctggctg atttccagaa attattgccg gaattcaatg tcgatcctgt cgaagttcgg
      781 atttaccgcc gcggacatcc catgttcatg ggcgtcccag ggcagttcac caaaaatcgc
      841 atcgctgcgg cgcatcccat ggaccggatt tatttcagcg gcaacgattc cggcgggccg
      901 gaatcgctga ccagcgaagc gattcgtttc tcgcagggcg gcgctgaatg ggcggaaatg
      961 atcctgacgg gcaaaccggg cgccaaggat ttcgccgaaa aagttctcaa cgcgcgtgaa
     1021 ttctgaatct cacttttctc atttctgtac tggacctgca gcggaatagg tatcgccctg
     1081 ttcgcatttg gtgtatacct tctgcaacca ggcgtcccat tcaaaattga gtctacaa
//
LOCUS       DATCBB010000896         1163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_287362, whole genome shotgun sequence.
ACCESSION   DATCBB010000896 DATCBB010000000
VERSION     DATCBB010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287362"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VII37_09820"
     CDS             <1..1078
                     /locus_tag="VII37_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5070079.1"
                     /translation="PPIPWDTIDLKQFNIVPKFFKSMLQKIGGGWGTFRIIPVESGRG
                     CPYGCEFCTVTGFFGDSIRFRTNESVVNELLLLKARAKKERGQIAVFFIDDNFAINIK
                     RTKSLLRDIIAAEAQVHWVAQISANLLRDEELVDLIAAAGGKWIFIGMESIDPANLKD
                     VNKGFNKPGEYAAVLQRLADRNVFAITSFIFGMDNDTTGVAERTLQQIRTWPPGLPIF
                     GLLTPLPATPLYKRLEAAGRLTRPKHWQEFIPFAMAHTPLKMTIEEAHAEVKYGWSKS
                     YSPEAIAKALAAVAHKPLGYRVNILISRLCFRGIYFPQLGRFAWVKTIAENRRTIFSL
                     IREGFGARRAARSGSLSLSSTAPH"
BASE COUNT          270 a          311 c          324 g          258 t
ORIGIN      
        1 cccgccgata ccgtgggata ccatcgacct gaaacaattc aatatcgtgc caaagttttt
       61 caaatccatg ctgcaaaaaa ttgggggcgg ctgggggaca ttccgcatca ttccggtgga
      121 gtccgggcgc gggtgccctt atgggtgcga gttctgcacg gtgacaggat ttttcggcga
      181 ctccatccgc tttcgcacca acgaaagcgt agtgaacgag ctgttgctgc tgaaagcgcg
      241 ggcgaaaaaa gagcgcggac agatcgcggt attttttatc gacgacaatt ttgctattaa
      301 catcaagcgg accaagtcgc tgctgcgcga cattattgcc gctgaggcgc aagtgcattg
      361 ggtggcgcaa atcagcgcca acctgctgcg cgatgaagag ctggtggatt tgattgccgc
      421 cgcggggggc aagtggatat ttatcggcat ggaatccatc gatcccgcca acttgaaaga
      481 cgttaacaaa ggttttaata agcctggcga atacgcggcc gtgcttcagc gccttgcaga
      541 ccgcaacgtg ttcgcgatta cttcgtttat tttcgggatg gacaacgaca cgaccggggt
      601 ggcggagcgt accttgcagc agattcggac ttggccgccg ggattaccta tcttcgggct
      661 gctcacgccg ctaccggcaa cgccgctata taagaggttg gaggccgccg gaaggctgac
      721 gcggccgaaa cattggcaag aattcatacc gtttgccatg gctcatactc ctctgaagat
      781 gaccattgaa gaagcgcacg ccgaggttaa atacggctgg tcgaaatcat atagcccgga
      841 agctatcgca aaggccttgg ctgcggtggc gcacaagccg ttgggatacc gcgtaaacat
      901 actcatatcg cggctttgct tccgaggaat ttacttcccg cagctcggtc gcttcgcgtg
      961 ggtcaaaacc atcgctgaaa accggcggac catcttcagc cttatcagag aaggtttcgg
     1021 ggccaggcgc gcagcgcgtt caggctcgtt gagcctgtct tccacagcgc cacattaatc
     1081 accaaaccga atcgcagcaa cttagagtca ttccttcgta gccgcccctc tcatagattt
     1141 ttgcgattcg ataaacacga att
//
LOCUS       DATCBB010000897         1728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_384506, whole genome shotgun sequence.
ACCESSION   DATCBB010000897 DATCBB010000000
VERSION     DATCBB010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384506"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="rplD"
                     /locus_tag="VII37_09825"
     CDS             <1..436
                     /gene="rplD"
                     /locus_tag="VII37_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY5070080.1"
                     /translation="SAKLTEQRLTVVEDWELENHKTKEFLGVLGKLKHKKSALIVAHG
                     ENTNLERASRNIEGVTLVAPNGLQPYDLLKHDLLVLSKEAVARLSHSLDPEKTPAATP
                     DVETIAPAPKREKKAAAKPAAAKKSKAAAKKPAKTAKKKGKE"
     gene            440..736
                     /locus_tag="VII37_09830"
     CDS             440..736
                     /locus_tag="VII37_09830"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY5070081.1"
                     /translation="MSTPHYQIIRRPIITEKGLGVKETQHTVVFEVSAKATKTQIKEA
                     VQQIFSVKVTDVRTANFYGKMRRRGQNEGYRRDWKKAYVKLAEGEKMIEYAENL"
     gene            791..1609
                     /gene="rplB"
                     /locus_tag="VII37_09835"
     CDS             791..1609
                     /gene="rplB"
                     /locus_tag="VII37_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY5070082.1"
                     /translation="MGLKSFNPYTASRRFITVVDKSHITKQEPEKALLEPKKRTGGRN
                     NHGEIVVWHRGGGHKKQYRQVDFRRDKLGVPATVAAIEYDPNRSSNLALLHYADGEKR
                     YILHPVGLEVGATVSTGEGADILPGNALQFKHIPPGTMVHNLELYPGRGGQVVRSAGG
                     SAQLLSKEGDLALVKLPSGEVRKFSIECMATVGQVGNLDHENETFGKAGRTRWRGIKP
                     TVRGVAMNPVDHPHGGGEGRVKGNHPQTPWAFPTLGKKTRNNKRTDRNIVERRK"
BASE COUNT          470 a          445 c          524 g          289 t
ORIGIN      
        1 gtcggcaaaa ctcactgaac agcggctgac cgtcgtggaa gactgggagt tggagaacca
       61 caagaccaaa gaatttttgg gtgtgctggg caaactgaag cacaagaagt ccgcgttgat
      121 cgtcgcacat ggcgagaaca ctaatctgga acgcgccagc cgcaacattg aaggagtcac
      181 gctggtagcg ccaaacgggc ttcagcctta cgacttgttg aagcacgacc tgttagtgct
      241 ctcgaaggaa gctgtggcgc ggctaagcca ttcgctagat ccggaaaaga ctccggcagc
      301 aactcccgac gtggaaacca tcgcgccggc gccgaagagg gaaaagaaag cggcagcgaa
      361 acctgcggcc gccaagaaat ccaaagcggc tgccaagaaa ccggccaaga ccgccaagaa
      421 aaagggcaag gaataactga tgtctacgcc tcactaccag atcattcgcc gcccgatcat
      481 cacggaaaaa gggttaggcg taaaagagac gcagcacact gtggtttttg aagtttccgc
      541 gaaagccacc aagacgcaga tcaaggaagc ggtacagcag attttcagcg taaaagtgac
      601 ggacgtacgt accgccaatt tttacggcaa gatgcgccgc cgcggacaga atgaaggtta
      661 ccggcgcgac tggaagaaag cctacgtgaa gctggcggaa ggcgaaaaga tgattgagta
      721 cgctgagaat ttataaatat cgggccactt tttttaaagc agcaagaaac gaaaagagta
      781 agaggcagcg atgggactga aaagctttaa tccatatacg gcgtcgcgac ggtttattac
      841 cgtcgtggat aaaagccata tcaccaagca ggagccggag aaggcgctgc tggagccaaa
      901 aaagcgtacc ggcggccgca acaatcacgg cgaaatcgtg gtatggcacc gcggtggcgg
      961 ccacaagaaa caatatcgcc aggtggattt ccggcgcgac aagctgggcg tgcctgccac
     1021 ggtagcggcc attgagtacg accccaatcg cagctcgaac ctggcgctgt tgcactacgc
     1081 cgatggcgag aagcgttaca ttctgcatcc ggtagggctc gaagttggcg ccaccgtttc
     1141 taccggcgaa ggtgcggaca ttcttccggg caatgcgctg cagttcaagc acattccgcc
     1201 gggcactatg gtgcataacc tggaacttta tccgggacgc ggcggccaag tggttcggtc
     1261 ggcgggcggg tcggcgcagt tactcagcaa ggaaggcgat ctggcgctgg tgaagttgcc
     1321 gtctggcgaa gttcgcaaat tttccatcga atgcatggcc accgtcggcc aagttggcaa
     1381 tctcgatcac gaaaacgaaa cgtttggcaa agcgggacgc actcgttggc gcggcattaa
     1441 gcccacggta cgcggcgtgg ctatgaaccc ggttgaccat ccgcacggag gtggggaagg
     1501 ccgcgtgaag ggcaatcatc cgcaaactcc ctgggctttc ccgacgttgg gtaaaaagac
     1561 gcggaacaac aagcgcactg acagaaacat tgtcgagcgc cggaagtaag cgcaaagagt
     1621 ttcagcgagc agcgagcagt tgtaactgag ggagaatccg gggagcttac acacagcggc
     1681 ccggcgagag cagatgatgg cgaccagagc agcacgttca tcaaagtc
//
LOCUS       DATCBB010000898         1133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_223907, whole genome shotgun sequence.
ACCESSION   DATCBB010000898 DATCBB010000000
VERSION     DATCBB010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223907"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..>1133
                     /locus_tag="VII37_09840"
     CDS             105..>1133
                     /locus_tag="VII37_09840"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY5070083.1"
                     /translation="MSQPANVELKQIPTGVRGLDDILRGGIPKFSFNIIAGSPGSGKT
                     TLAHQIVFANATTKKPALFFTVLGEPAIKMLRYQQQYSFFDESKLGKAIRFFNLADVV
                     LNQDLNAVYEEITKQVIAANPSIVVVDSFRTVVRKVVSGAGEIDMQSFIQRLAQFLAS
                     WEATTFLVGEYVPEEIRDNPIFTIADGLFWLSQNVERNSVVRKLQIWKVRGQDSVPGL
                     HTIRISDAGLQAFSRTLGFVGKRKLPARRRRLSMGIPDLDKMMGGGVLEGDSLLIAGP
                     SGTGKTALATQFIAAGLRNGESAIMAIFEERPKGYTDRADSFGLNLKTPLEKGKLEII
                     YLRPLDLSV"
BASE COUNT          256 a          310 c          321 g          246 t
ORIGIN      
        1 ttcaagttcg tctgcccttt ccaaggaacc atccgctttg acgtgcactg tgtgcagcga
       61 gtccagttct ttgcgcgaga ttgtgagggc gcgcaaaaaa agcggtgagc cagccggcta
      121 atgtcgagct caaacaaata ccgaccggcg tgcgcggcct ggacgacatc cttaggggcg
      181 gcatcccgaa attttctttc aacatcatcg ccggctcgcc cggaagcggc aaaaccacat
      241 tggcgcatca gatcgttttc gccaatgcca cgacgaaaaa acccgcgctc tttttcaccg
      301 tcctcggcga acccgctata aaaatgctgc gctaccagca acaatattct ttcttcgacg
      361 aatccaagtt gggcaaggcc atccgctttt tcaacctggc cgacgtggtg ttgaaccagg
      421 atttgaacgc ggtttacgag gaaatcacca aacaagtcat cgcggccaat ccgagcatcg
      481 tggtggtgga ttcctttcgc accgtggtgc gcaaagtggt gagcggcgcg ggcgaaattg
      541 atatgcaatc gttcatccag cgcctcgccc agtttctcgc cagttgggaa gccacgacgt
      601 ttctggtcgg cgaatatgtt ccggaagaga ttcgcgacaa tccgatcttc accatcgccg
      661 atggtctgtt ctggctttcg cagaatgtgg agagaaattc cgtggtgcga aaattgcaaa
      721 tctggaaagt gcgcgggcag gactccgtgc cgggattgca caccatccgc attagcgacg
      781 ccgggttgca agccttctcg cgcacgctgg gattcgtcgg caaaaggaag ttgccggcgc
      841 ggcgccggcg gctttccatg ggcattcccg atttggacaa gatgatgggc gggggcgttc
      901 tcgaaggcga cagtctgctc atcgcggggc cttcggggac gggcaaaacg gcgctggcca
      961 cgcaatttat tgccgcggga ctgcgcaatg gagaatcggc aattatggcg atcttcgaag
     1021 aacgcccaaa aggatacacg gatcgcgccg acagttttgg tttgaattta aaaacgcctc
     1081 tggaaaaggg aaaactcgaa atcatttacc ttcgtccgct cgacctttcg gtg
//
LOCUS       DATCBB010000899         2810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_223918, whole genome shotgun sequence.
ACCESSION   DATCBB010000899 DATCBB010000000
VERSION     DATCBB010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223918"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1919
                     /locus_tag="VII37_09845"
     CDS             <1..1919
                     /locus_tag="VII37_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5070084.1"
                     /translation="VRSFFDTLREAGGTARLMLVRAAAAQWNKPVTDCHTELHEVIHT
                     PSGKKLGYGELVASAAKLEVPKKEDVKLKARTEWRYIGKGTPNYDLKDMCDGKAVYGQ
                     DTRMDGMLYASVAHPPVFGSTAKTLDDKAALAVAGVKQTASIEGFKPPVGYQTLGGVA
                     VLADSTWAAMQGKKKLKIEWEKSPHSVYNSDSYKKELQETARKPGKVARDNGNVDAIF
                     AKNGKVVEADYYVPLLAHASMEPPAALAVFRNGKVEVWAPTQNPQGAKDAIASAVGLK
                     KEDVTVNVTLLGGAFGRKSFPDFAIEAAVLAKKTGKPVKVVWSREDDIKFDTYHSVAA
                     MYMKAALGADGKPEAWLQRTVFPPIATTFDPKANHAPADELGLGFTDVPFALPNHRVE
                     NGPATAHVRIGWLRSVANIYHAFAVHSFADELAHAAGRDSLDYLLQLLGPDRVIPKEE
                     FPKDYPNYDDAYEQCPIDTARFRRVLALAAEKAGWGKQKSGNGFGMGVAMHRSFLTYV
                     ASVVQVQVNDAGQVQIKRVDTALDAGTVVNPEMVRQQFEGAAVMGTSLAFYGEITAKD
                     GAIEQSNFNDYQVARMKDAPRETYIHIIPSEDKPAGIGEPGLPPFAPALCNAIFAATG
                     KRVRELPLSRAGFA"
     gene            complement(1996..>2810)
                     /locus_tag="VII37_09850"
     CDS             complement(1996..>2810)
                     /locus_tag="VII37_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070085.1"
                     /translation="MHSGKEWFGGFGLPGAWLLLSLILCTCQLVAGAQDHPAHESHDG
                     QAGMSNDMPGMDMSSKAPDTEAWMFTPEKLAADKRESEFNHHLAGFFVVLAGIFILAE
                     PRLRARWPNVRYAWPVCFLLAGIFVLVFSDTELWPFGPQSWFYGLSNHMEVRQHKSFA
                     LILLVVGVIEFLRARGSLKAAWAGWVFPVVAIAGSIILIFHEHQGGMHGAGHMERMAR
                     IQTEHHSYTAAGFGIGLTKGLSEIKTNWQPVFNKLWPSIMIVLGVMLMFYVE"
BASE COUNT          743 a          874 c          676 g          517 t
ORIGIN      
        1 ccgtgcgcag tttcttcgac actttgcgcg aagccggggg aaccgcccgt ctaatgctcg
       61 tccgcgccgc cgccgcccag tggaacaaac ccgtcaccga ctgccacacc gaattacacg
      121 aagtaataca cacgccttcc ggcaaaaaac ttggatacgg tgaactggta gcttccgcgg
      181 caaaactgga agttccaaaa aaagaagatg taaaactgaa agcccgtacg gaatggcgct
      241 acatcggaaa aggcacacca aattacgacc tcaaagacat gtgcgacggc aaggccgtct
      301 acggtcaaga cacccgcatg gacggaatgt tgtacgccag cgttgcacac ccacccgttt
      361 tcggcagcac cgcaaaaacc ttggacgata aagccgcact cgctgtcgca ggagtaaagc
      421 agaccgcctc aatcgaagga ttcaagcccc cagtcggcta ccagactctg ggaggcgttg
      481 cagttctagc ggacagcacc tgggccgcca tgcaaggcaa gaaaaaacta aaaattgagt
      541 gggagaaaag tcctcacagc gtctataact ctgactccta caaaaaagaa ttacaggaaa
      601 ccgctcgcaa acccggaaaa gtagcgagag ataacggaaa cgtggacgcg atctttgcca
      661 agaacggcaa agtcgtggaa gccgattatt acgttccgct cctcgcgcat gcctccatgg
      721 aaccgccggc ggctcttgct gttttccgca acggcaaagt cgaagtctgg gcccccacgc
      781 agaatcctca aggcgccaaa gatgccatcg ccagtgccgt cggattgaaa aaagaagacg
      841 tcaccgtaaa cgtcactctg ctgggcggag cttttggccg caaatcattc ccagattttg
      901 cgattgaagc cgcggtgctc gcaaaaaaaa cgggcaagcc ggtaaaagtc gtctggagcc
      961 gcgaagacga catcaaattc gacacctatc actcggtagc cgctatgtac atgaaagccg
     1021 cgctgggtgc agacggcaaa ccagaagcct ggttgcagcg caccgtgttc ccgccgattg
     1081 caaccacctt cgatcccaaa gcaaatcatg ccccggctga tgaattaggt ctgggcttca
     1141 cggacgtgcc cttcgccctg cctaatcacc gcgtagaaaa cggccccgca accgcccacg
     1201 tgcgcatcgg ctggctgcga tcagtcgcaa atatttatca cgccttcgca gtccattcct
     1261 tcgccgacga actcgcacac gccgcgggac gcgattcact cgactatcta ctacaacttc
     1321 tcggaccaga tcgcgttatt cccaaggaag aattccccaa ggattacccg aattacgacg
     1381 acgcctacga gcagtgcccc atcgacaccg ccagatttcg gcgcgtccta gccctagccg
     1441 cagaaaaagc cggctggggc aaacaaaaat ccggcaatgg atttggcatg ggcgtcgcca
     1501 tgcaccgcag tttcctgact tacgtcgcct cggtagtgca agttcaggtc aacgacgccg
     1561 gccaagtgca aatcaaacgc gtcgacaccg ccctggacgc cggaacggtc gtaaatccgg
     1621 aaatggtgcg ccaacaattt gaaggagccg ccgtgatggg cacctccctc gcgttttacg
     1681 gtgaaatcac cgcgaaggat ggagccatcg agcaatccaa tttcaatgat taccaggtcg
     1741 cccgcatgaa agatgcgcca cgcgaaacct acatacacat catacccagt gaggacaagc
     1801 ccgcgggaat cggcgagccc ggattgccgc cgttcgcgcc cgcactctgc aacgccatct
     1861 ttgcggccac cggcaagcgt gtccgcgaat tgccattaag ccgcgcgggc ttcgcctgaa
     1921 attctgttga atggtatttt gttaaatttg ccggacgcga agagtgctaa tttccagagc
     1981 gaaaatctcc attaactatt ccacgtaaaa catcaacatc acacccaata cgatcatgat
     2041 cgacggccac agcttattaa aaacaggctg ccagttcgtt ttgatttcag acaagccctt
     2101 cgtaagccca ataccaaatc ctgccgccgt atagctgtga tgctccgtct gaatgcgcgc
     2161 catgcgctcc atatgccccg cgccatgcat ccccccttga tgttcatgaa aaatcaaaat
     2221 gatggatcca gcaatcgcca caacaggaaa aacccaaccc gcccacgctg ctttcaaact
     2281 cccacgagcg cgcaaaaatt caatcacgcc aacgaccagc aggatcagcg caaatgattt
     2341 gtgttgccgc acttccatgt ggttcgagag cccgtagaac cacgactgcg gcccaaacgg
     2401 ccacagctcc gtatcgctga aaaccagcac aaaaatcccc gcaagaagaa aacataccgg
     2461 ccacgcatag cgaacgttcg gccatcgcgc cctcagccga ggctccgcaa gaataaaaat
     2521 ccccgccagt accacaaaaa aaccagccag gtgatgattg aattcgctct cgcgtttgtc
     2581 cgccgccaat ttttcgggcg taaacatcca cgcctcagtg tctggggcct tcgaactcat
     2641 atccattccc ggcatgtcgt tgctcattcc cgcttggcca tcgtggctct cgtgcgccgg
     2701 gtgatcttgc gcgccggcca ccagctgaca cgtgcaaaga atcaagctaa gcagcagcca
     2761 tgcgcccggt aatccaaatc ccccgaacca ttccttacct gaatgcattg
//
LOCUS       DATCBB010000900         1746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_93059, whole genome shotgun sequence.
ACCESSION   DATCBB010000900 DATCBB010000000
VERSION     DATCBB010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93059"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VII37_09855"
     CDS             <1..874
                     /locus_tag="VII37_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY5070086.1"
                     /translation="ERYRVVIDALRGDAALWSDVVEIEANEARRSDIQACHTPQHFKH
                     VEEAVREGKGYLDNDTLVSGKSLEAALRGAGAACQAIDAVMEGAAKNAFVPVRPPGHH
                     ATPDRAMGFCLFNNVAVAARYAQKQYKEIARVAILDWDVHHGNGTQGIFYDDPSVFYF
                     SMHQYPWYPGTGSRGETGQGRGRGYTLNVPLRALTPTKDQRRAFESAIEEIAREFTPD
                     LVIISAGFDAHKGDPLGQLLLSDEDFVKMTGVVKEWANEACAGRVISCMEGGYNLDTL
                     GETVRAHVRELQRG"
BASE COUNT          381 a          475 c          534 g          356 t
ORIGIN      
        1 tgagcgttac cgcgtggtga ttgatgcgtt gcgcggtgat gccgcgctct ggtccgacgt
       61 cgtcgagatc gaagccaatg aggcgcggcg cagcgacatt caagcctgcc acacacctca
      121 gcatttcaaa cacgtggagg aggcagtccg cgaaggcaag ggctatttgg acaacgacac
      181 gttggtttcg ggaaagtcgc tggaggcagc gttgcgcggc gcgggcgcag cgtgccaggc
      241 gatcgacgcg gtaatggaag gcgcggcaaa gaacgctttt gtgcccgtgc gtcctcccgg
      301 acatcacgcg acgccggacc gagcgatggg gttttgcctc tttaacaacg tagctgtcgc
      361 ggcgcgctat gcgcagaagc aatacaaaga aattgcgcgt gtggcgattc ttgattggga
      421 tgtgcatcac ggcaatggca cccagggtat tttttatgac gacccttcgg tcttctactt
      481 ttcgatgcat cagtatccct ggtatccggg cacgggcagt cgcggtgaga cgggccaggg
      541 gcgcggccgt ggctacacgc tcaacgtgcc tttgcgcgca ctcacgccga caaaggatca
      601 gcggagagct tttgaatcgg cgatcgaaga gattgcgaga gagttcacgc cggatctggt
      661 gatcatctcg gccggctttg acgctcacaa aggagatcca ctcggacagt tgctcctcag
      721 cgacgaagac tttgtgaaga tgacaggggt cgtgaaggaa tgggcgaacg aagcgtgcgc
      781 gggccgcgtc atctcttgca tggaaggcgg atacaacctg gatacgctgg gcgagacggt
      841 tcgggcgcac gtgcgcgagc tgcagcgtgg ttaatgttcg acaaacttca ctaatcccga
      901 acaagttgaa gcttgcaaaa cccgcgtagc gggtggaagc ataaagcctg gggtggagcg
      961 gagcgaaacc ccaggatcac gagaggtgag aaaccgagca cgcgaagcgt gcgatagccc
     1021 aagcgctgga atttactcgg gatgaatggc tgtcgcccac ttcatgggct cgtccttggt
     1081 cttacaacga tcctggggtt ccgctccgct ccaccccagg ctttatgcta tcgccgcgct
     1141 tcgcgggctc agggcaaact ctacaccacc ctcttcagag cttcctttag tttgccaaag
     1201 ttttctgtac accttcacct tcctggagta aactttgcgc accagcctac agaggtaaac
     1261 ctcaagacgt ttttctcggg agacagcgat gccagatctt gaaattactt gcgccgagtg
     1321 cggaaatacc ttccctttta ccgaaagaga acaggactac taccgcgagc gtggcctcag
     1381 ccatccgaag cgttgtaaac cctgccgtga tgcccgccgc cagaactttg gcggagagcg
     1441 acagggaggg ggagcggaac gcgaaaagtt tgagattgtc tgcgatacgt gcggcaagcc
     1501 cgacagtgtg cccttcaaac cagcgccggg ccggccggtg ctctgtagtc aatgtttcag
     1561 cgccagccgc gcgcagaacc gcggggcgtg aacgattatt gcgacgccgc cgatgattag
     1621 gccattcaga acagcggcgc aaacctaact caggttccac tcacatgcgt tgcttcgcac
     1681 cgtaggtgcg aaatgtttat agcctgacgc tactgtttga tgttgtttgc ttcgaaggag
     1741 cgaaat
//
LOCUS       DATCBB010000901         1164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_60963, whole genome shotgun sequence.
ACCESSION   DATCBB010000901 DATCBB010000000
VERSION     DATCBB010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60963"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VII37_09860"
     CDS             <1..425
                     /locus_tag="VII37_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY5070087.1"
                     /translation="TAMNDEELLVADFRATGLTVGPHPMQYRREEMKRMGIYSAADLA
                     HLSHGRRLRIGGCVIARQRPGTAKGMMFMSLEDETGIANAIIAPDLLQKNRVLLISER
                     FVMVEGILQNQDNVIHIRAEKVFPLSVTRAETVSHDFH"
     gene            complement(466..>1164)
                     /locus_tag="VII37_09865"
     CDS             complement(466..>1164)
                     /locus_tag="VII37_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEY5070088.1"
                     /translation="TQSQAAQIRASAVNPHPEDLPEDELTSQIVRGCLIARDALFNVR
                     DFLAEGSRLAFLAIKDCEKELDQIERKIDEQLPEAITRVGEAKARELLACLKFITDLE
                     RIGDLAYNAVMHLQPRREKLPVADSRQLVEMASLLYTMLEQIHQGFIKRDVSYAQSVL
                     QSDPEVDRLCHALFQRHLAEPNSPSGQKNFDILLAAQALERIGDHSKNLAEELYGLTE
                     GRSLRHNTRRLSTA"
BASE COUNT          250 a          299 c          351 g          264 t
ORIGIN      
        1 tgacggcaat gaacgatgag gaactgctgg tggccgattt tcgcgctacc gggctgacgg
       61 tcggtccgca tccgatgcag tatcgccggg aagaaatgaa gcggatggga atttatagcg
      121 ccgctgatct ggcgcatttg tcccatgggc gccggttgcg aatcggcgga tgcgtgatcg
      181 cgcgccagcg tcccggaacc gcgaagggaa tgatgttcat gagtcttgaa gatgaaacgg
      241 gaattgccaa tgcgatcatc gcgcccgatc ttctgcagaa aaatcgcgtg ctgctgattt
      301 cagagcgttt tgtgatggtg gagggaattc tgcagaatca ggacaacgta attcacatcc
      361 gggcggaaaa agttttcccg ctcagcgtca cccgagccga gacagtatcc cacgactttc
      421 attgagacct gaatccgcgt tgggaaacaa tgtcgagcag cggcgtcaag cggtcgaaag
      481 tcttctggtg ttgtgccgca gcgaacgtcc ttcggtcagg ccatagagtt cttcggcaag
      541 attcttggag tgatcgccaa ttcgctcgag cgcctgggca gcgagcagga tatcgaaatt
      601 tttttgtcca ctcgggctgt tgggctcggc tagatgccgc tgaaacaggg cgtggcacag
      661 tcgatctact tccggatcgg actgcaacac gctttgcgca taggagacat cgcgcttgat
      721 gaatccctgg tggatttgct ccaacatcgt gtacagcagc gaagccattt ccacgagctg
      781 gcgcgaatcc gccactggca atttttcgcg acgcggttgc aagtgcatga cggcgttgta
      841 ggcgagatct ccgatgcgct caaggtcggt gatgaacttc aaacatgcga gcaattcacg
      901 agccttggct tcacctacgc gggtgatggc ttcgggaagc tgctcgtcaa tcttgcgctc
      961 gatctggtcc aactcttttt cgcaatcctt aatggcgaga aatgccaggc gcgatccctc
     1021 ggcaagaaaa tccctgacgt tgaatagggc gtcgcgggcg atcaggcagc cacgaactat
     1081 ctgggacgtg agttcgtcct cgggcaaatc ttcaggatgc ggattgacgg cactcgcacg
     1141 tatttgcgct gcctgggatt gcgt
//
LOCUS       DATCBB010000902         1042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_159029, whole genome shotgun sequence.
ACCESSION   DATCBB010000902 DATCBB010000000
VERSION     DATCBB010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159029"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VII37_09870"
     CDS             complement(<1..765)
                     /locus_tag="VII37_09870"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEY5070089.1"
                     /translation="MHSVVIDKTEPAYQELLGRPIRELGLKLAGSPVERFVEQLYREL
                     EAKGLAKFRPEVYLTDEWGCPSGEPIIGIPFYLASAALAQLEKETHDLEDAREIMMYL
                     RHEAGHAFTYAYKLHNKPEWKQIFGPFRRPYRDNYRPAPFSRDYVRHLPGWYAQKHPD
                     EDFAETFAVWLTPRSNWRKRYRGWAAIDKLKYMNRLARQLGKTDPPRRRGQTDITVDE
                     METTLSEFYHLQRAAREEVAVSELTPDTDLRDIFPVS"
BASE COUNT          243 a          337 c          250 g          212 t
ORIGIN      
        1 cgacaccgga aaaatatcgc gcaaatccgt gtccggcgtc agctccgaca ccgcaacttc
       61 ttcccgcgcc gcccgctgca agtggtaaaa ctccgacagc gtagtctcca tctcatcaac
      121 ggttatgtcc gtctgtcccc gccgccgcgg aggatccgtc tttcccaact gccgcgccag
      181 ccggttcata tacttcaact tgtcgatcgc ggcccatccg cgataacgct tgcgccagtt
      241 cgaacgcggc gtcagccaaa ccgcaaacgt ctcggcaaaa tcttcatccg gatgcttctg
      301 cgcataccat cccggcaaat gccgcacata gtcgcgcgag aacggagccg gccggtaatt
      361 atcgcgatac ggccgccgga acggcccaaa aatctgcttc cactccggct tgttgtgcag
      421 cttgtacgcg tacgtaaacg catgccccgc ctcatgccgc aggtacatca tgatctcgcg
      481 cgcatcctcg agatcgtgcg tttctttttc cagttgcgcc agcgccgcac ttgccagata
      541 aaacggaatc ccgataatcg gctcgcccga agggcatccc cactcatccg tcaaatagac
      601 ttcgggacga aacttagcca gccccttggc ctcgagctcg cgataaagct gctccacaaa
      661 ccgctccacc ggagacccag caagcttcag ccccagctcg cgaatcggcc ggccaagcag
      721 ttcctgatac gcgggttcag ttttatcaat gacgacgcta tgcacaaacg ctcgctaaca
      781 aaattctgca accgcgccag cacacacggg gagaggcagt gtcaccaaga ttaacagtaa
      841 tagaagcagg aattgtacgg aagtaaccgg ggtaatgctg cgcgcggcgg tttttcaacg
      901 gcaattatgc tttcgtggta aatctcaagc tgacttttcc gctcttgctg atgctcgcaa
      961 tgaaaggggc atcatatctg cgaaagaatc ttgccatctc tcgcaatgca gagacaaaaa
     1021 tcgttgccat ctcggcaccg ct
//
LOCUS       DATCBB010000903         4426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_60971, whole genome shotgun sequence.
ACCESSION   DATCBB010000903 DATCBB010000000
VERSION     DATCBB010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60971"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VII37_09875"
     CDS             complement(<1..677)
                     /locus_tag="VII37_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5070090.1"
                     /translation="MLAFTVKNKKLVARNTPVPKLRPRWALVRVLLAGICNTDVEILR
                     GYHSFRGVPGHEFVGEVADVNGVSAAEKTKWLGKRVSGEINVSCSAYNYRPVCASCRR
                     GLKTHCARRTVLGIVNHDGAFAEYLALPLENLHLIPNSITNEQAVFVEPLAAACEILD
                     QINVKQFREAAVLGDGKLAQLIARVLRSALPRVVMYGKHKDKLQQAAAARIDTQIVRG
                     DAVDLKRV"
     gene            complement(721..2199)
                     /gene="selA"
                     /locus_tag="VII37_09880"
     CDS             complement(721..2199)
                     /gene="selA"
                     /locus_tag="VII37_09880"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY5070091.1"
                     /translation="MDARKPRKHRAYRIAVTKPAISTEQAELLRRIPSVDELLLRPRL
                     AAYAKRMDRDQLVVLLRDVLSALRAKIAAPQFLSPAVTVTALDFDSIEELIAAEADRV
                     LRPSLQPVINATGVILHTNLGRAPLAAAVVEAIKNAATQYSNLEYNLESGARGKRDDH
                     TNELLARLTGAEDAIVVNNCAAAVLLVLAALAKAGEVIVSRGELIEIGDGFRIPEIMA
                     QSGAILREVGTTNRTRLRDYESAINENTRVLLRVHPSNFKITGFAEKPTLAELAGLSE
                     RSSVPLVEDLGSGSLVDLGASGFAEPTVRHSIEAGVSLVMFSGDKLLGGPQAGIIAGK
                     KDLVQRVRRHPLFRALRVDKLTIAALEVTLNAYLRSAWAEIPAQRMIRMTAQELQQRA
                     ENFLRQVRSLVPLSDAKMEIVDGFSLAGGGSTPDQSLPSKIIRVSSAKYPAAKLEQRL
                     RRPANGVSVIARVEENRVILDLRTVFPEQEPQLAASLATALS"
     gene            2342..2989
                     /locus_tag="VII37_09885"
     CDS             2342..2989
                     /locus_tag="VII37_09885"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEY5070092.1"
                     /translation="MAVTTINAIDEELNQFEKDIRQLKIEYEQYFGGGRKRPPTEIEW
                     RLELMAKRYAERSGELKYGQRFRYNNLTQTYSKYKDIFRKRLQQKEEGKVSRHFGSAA
                     KKIEAERAQKHAETVLKADAAESKKSDDKALATFRVVCSKPEQEAEKISKLYEAFLEA
                     KQKAGEDTIKFTRSNFHEFVMKKTKDLQKTKNCKDVEYVVEVTEGHVKLKALVKI"
     gene            3162..>4426
                     /gene="purH"
                     /locus_tag="VII37_09890"
     CDS             3162..>4426
                     /gene="purH"
                     /locus_tag="VII37_09890"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY5070093.1"
                     /translation="MAVQRALISVFDKTGVLEFAKRLHALGIEILSTGGTAKLLRDAG
                     VTVKDVSEFTGWPEMLGGRVKTLHPKVHGGLLFRRGHAEDQNQAAEHGIAPIDLVVVN
                     LYPFAETAAKAGLTAEELIENIDIGGPTMLRSAAKNFESVTVVTDPADYPRVAAELET
                     QRETSLATRLDLARKVFAATSSYDGMITTELERLSAKAPSIELSEKQALPERLHIALR
                     LQQPLRYGENPHQAAGLYVPARSTPSGLAAAKQLQGKELSYNNFVDLEAARSLAGEFS
                     RPAAVIIKHNNPCGAAEQETLLDAYLKALASDPVSAFGGVLAFNRVVDAATAEEVAKL
                     FVECIAAPGFDEKAKTVFAAKKNLRLLELPGNGLEPDRELQLKRILGGVLVQQPDLGE
                     IKEGELRTVTKRAASPEEMSTLRFAWKVS"
BASE COUNT         1005 a         1106 c         1412 g          903 t
ORIGIN      
        1 acgcgtttta ggtccacagc atcgccgcgc acgatttgcg tgtcgatccg ggctgctgca
       61 gcctgctgca atttgtcttt gtgctttcca tacatgacta cacgggggag cgcggagcgc
      121 aggacgcgcg cgattaattg cgcaagtttt ccgtcgccca ggacagcggc ctcgcgaaat
      181 tgtttgacgt tgatctggtc caggatttcg caggcggcgg ccagcggttc tacgaatacg
      241 gcttgttcgt tggtgatgga attgggaatg agatgaagat tctcaagcgg caacgccaga
      301 tattccgcaa aggctccgtc gtgattcacg atgccgagga cggtgcggcg cgcacaatga
      361 gttttgaggc cgcgacggca ggaggcgcaa acaggccggt agttatatgc ggaacaggaa
      421 acgttgattt ctccgctgac gcgtttgccg agccatttcg ttttttctgc cgcggaaacg
      481 ccattgacgt cggcgacttc gccgacaaat tcgtggccgg gaactccacg aaaggagtga
      541 tagccgcgaa gaatttccac gtcagtgttg cagataccag caaggagtac gcggacgagt
      601 gcccagcgcg gacggagttt tgggaccggt gtgtttcgcg cgactagttt tttattttta
      661 actgtgaagg ccagcatgga atttatcggc gcgcgaattt ttgtgcgcta aagttctgca
      721 ctaactcagc gctgtggcga gcgaggcggc cagttggggc tcctgttcag gaaacacggt
      781 gcgcagatcg aggataacgc gattttcttc tacgcgggcg atgacggaga cgccgttggc
      841 cgggcggcga aggcgctgtt ctaatttggc ggcgggatac ttcgcgctgg agacgcgaat
      901 aatttttgaa ggcaaagatt ggtctggcgt ggaaccgcca ccggccaaag aaaacccatc
      961 tacgatttcc attttggcgt cagagagcgg cacgagagag cgcacctggc gtagaaaatt
     1021 ttccgcgcgc tgctgtaatt cctgcgcggt catgcgaatc atgcgttgcg cggggatttc
     1081 cgcccacgcg gagcgcaggt aagcattaag ggtaacttcc agcgcggcga tggtgagctt
     1141 gtcgacgcgc aatgcgcgga atagcggatg acggcgcaca cgctgcacta aatctttttt
     1201 gccggcaatg atgccagctt gcggaccgcc gaggagtttg tcgccactga acatcaccag
     1261 ggaaacgccg gcctcgatgc tgtggcgtac ggtaggttcc gcgaaaccgc tggcgcccag
     1321 atcaacgagg gagcccgagc cgaggtcttc aacgagcggc acggaactgc gttcgctaag
     1381 cccggcaagt tcggcgagcg tgggcttttc cgcgaagccg gtaatcttga agttggatgg
     1441 atgcacgcgc agcagcacgc gagtgttttc gttgatggca gattcgtagt cgcgcaggcg
     1501 cgtgcggttg gtggtgccaa cttcgcgaag aatggcgccg ctttgcgcca tgatttccgg
     1561 gatgcgaaag ccatcgccga tctcgatcaa ctcgccgcga gaaacaatga cttcgccagc
     1621 cttggcgaga gccgctaaaa ccagcaacac ggctgccgcg cagttgttca cgacgatggc
     1681 gtcttccgca ccggtcagcc gcgccagcag ttcgttggtg tgatcgtcgc gcttgccgcg
     1741 agcgccggac tccaagttgt attcgagatt gctgtattgt gtggcggcgt tttttatcgc
     1801 ctcgaccacg gcggcagcca gcggggcgcg gcccaaattg gtatgcagga ttacgccggt
     1861 agcgttgatg accggctgca gggaaggccg aagcacgcgg tcggcttccg cagcgatcag
     1921 ttcttcaatg gaatcgaaat ctagcgcggt gactgtgacg gcagggctca ggaactgagg
     1981 agctgctatt tttgcacgga gtgcgctcag cacatcgcga aggagcacca cgagctgatc
     2041 gcgatccatg cgcttggcat aagcagccaa acgggggcgc aacagcagct cgtcgacgga
     2101 tgggatacgc cgcagtaact cagcctgttc agtggagatg gcaggtttcg tgactgcgat
     2161 ccggtaggct cggtgtttac ggggttttcg ggcgtccacc gctgtatttc agcacagggc
     2221 agcggtccag ggcaacggga gcagaggcca caatgcggta ttcgattcgc acgaaactcc
     2281 gcgagtacga ttgaccgcag cacaagctgc ttctagcata ttagttagag ggtgtcccgg
     2341 cgtggccgta acgaccatta acgccattga tgaagaattg aaccagttcg aaaaagacat
     2401 tcgccaattg aagatcgagt atgagcagta ttttggcgga ggacgcaagc ggccaccgac
     2461 ggaaatcgag tggcggctcg aactgatggc gaagcgctat gcggagcgca gcggcgagct
     2521 gaagtacggg cagcgcttcc ggtacaacaa tctcacgcaa acttattcaa aatataagga
     2581 catttttcgc aaacggctgc agcagaagga agaaggcaaa gtgtcgcggc actttggttc
     2641 agcggccaaa aagattgaag cggagcgcgc gcaaaagcac gcggaaacgg ttttaaaggc
     2701 cgatgccgca gagagcaaga agagcgacga caaagccctc gcaacgtttc gtgtggtgtg
     2761 ctcaaagccg gagcaggagg cggagaagat cagcaagctt tatgaggcgt ttttagaggc
     2821 caagcagaaa gcgggcgaag acaccatcaa atttacgcgc tcgaattttc acgaatttgt
     2881 gatgaaaaag acaaaagacc tgcagaaaac caagaattgc aaagacgtcg aatatgttgt
     2941 ggaagttaca gaggggcacg tcaaacttaa agcgctggta aagatttaat tcatttccaa
     3001 aagcatttta cggattgccg gatccttcct gaagtcctca tttcactctg aaagccgaac
     3061 cgaaccaaac aagaacgggc gcaatgaaat tgccaccgtt ctttgctttc gccggtgcgc
     3121 ggcgcttaca ctgttgacct ttcgtatcgg gagggaagca gatggcggtg cagcgcgcgt
     3181 tgatcagcgt attcgacaaa accggagtat tggagtttgc aaagcggtta catgcgctgg
     3241 gcattgaaat tctttctacg ggtggtacgg cgaaattgct gcgtgacgct ggtgtcaccg
     3301 tgaaggatgt ttcggaattt accggctggc cggaaatgct gggcggacgc gtcaaaacgc
     3361 tacatcccaa agtgcatggc ggcttgttat tccgtcgcgg gcacgcggaa gatcagaatc
     3421 aagcggcgga acatggaatc gctccaatcg acctcgttgt tgtgaatctc tatcccttcg
     3481 cggaaacggc cgcgaaagcg gggctcacgg cggaagagct gatcgaaaat atagatattg
     3541 gcgggcccac gatgttgcgg tcggcagcaa agaattttga aagcgtgacg gtggtgacgg
     3601 atcccgcgga ttacccgcgt gtggccgcgg aactggaaac gcagcgggaa acgtcgctgg
     3661 cgacgcggct ggatctggcg cgcaaagttt ttgcggccac gtcgagctac gacgggatga
     3721 ttacgaccga gctggagcgg ctttcggcca aagcgccgtc gattgagctg agcgagaaac
     3781 aagctttgcc ggagcgtttg catatcgctt tgcggctgca gcaaccgctg cgttatgggg
     3841 agaacccgca tcaggccgcc gggttatatg ttccggcgcg tagcacgcca agcgggttgg
     3901 cggcggcgaa gcaactgcag ggcaaggaac tttcctacaa taattttgtc gatctggagg
     3961 cggcgcgcag tttggccggg gaattctcgc gcccggccgc agtgattatc aaacacaaca
     4021 atccctgcgg agcggcggag caggagaccc tgttggatgc gtacctgaag gccttggcga
     4081 gcgatccggt ttcggcgttt ggcggggtcc tggccttcaa ccgggtggtg gatgccgcga
     4141 cagcagaaga ggtcgcaaaa cttttcgtgg agtgtattgc cgcgccgggg ttcgacgaaa
     4201 aagcgaagac cgtttttgcg gccaagaaaa atttacgttt gctggagctt ccggggaacg
     4261 ggttggagcc ggaccgggaa ctacaattga agcggattct gggaggcgtg ctggttcagc
     4321 agccggacct gggagaaatt aaagagggcg agctgcgcac ggtcacgaaa cgggccgcgt
     4381 cgccggaaga aatgagcacc ctgcgttttg catggaaagt ttcgaa
//
LOCUS       DATCBB010000904         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_159076, whole genome shotgun sequence.
ACCESSION   DATCBB010000904 DATCBB010000000
VERSION     DATCBB010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159076"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VII37_09895"
     CDS             complement(<1..570)
                     /locus_tag="VII37_09895"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEY5070094.1"
                     /translation="MLQFAIWKMDGEACVGYRDLREFVARLEKEGELKRVRAEVDPVL
                     EITEVAQRVARSKTRAAHSVGPALLFEKPKGSKVPLLINTFGSVRRMELAFEVDKLED
                     VAARIQGFLKMETPQGLFDKIKMLPKLAELGSFFPKSVKSGACKDVIRKGADVNLLEF
                     PILKCWPQDGGRFITFPLVFTKNPETGKRN"
     gene            complement(666..>2154)
                     /locus_tag="VII37_09900"
     CDS             complement(666..>2154)
                     /locus_tag="VII37_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5070095.1"
                     /translation="PWSRYNSVLPGQPADPQRDQFVRGETDAGALPSDDADIAYAPVT
                     RLSRWIEQRKLLSERLTKIYLRRLENFDPKLRCVITLTREHALEQARKADQEIAAGKY
                     RGPLHGIPWGAKDLLDTAGIATTYGAEFYRNRIPKEDAAVVKRLDDAGAVLVAKLSLG
                     ALALNDIWFGGQTMNPWLLEEGASGSSAGPGAATAAGLVGFAIGSETGGSIVSPSMRC
                     GVCGLRPTFGRVPRSGAMTLCWSLDKLGPMTRRVEDALLVLHAISGADPGDNSSVASH
                     LQFDANANVNGLRVGYFPRWMKERPATDVDRAGLAMLPKLGMVPVEVALPDWPYQSLN
                     LILFAEGAAAFEELTLNHELDQLKVQTPDAWPNLFRQARFLSAVDFVQADRLRRKVAE
                     EMARIFSKVDVLLVPSLRDEMLTISNHTGHPSITIRAGFVEVSEARSDWAPDPQHPLP
                     KFSTPRRVPHGVTLIGRLFEEGTLGRAGIALERAFHVAEERPPLF"
BASE COUNT          492 a          751 c          475 g          436 t
ORIGIN      
        1 gttgcgcttt ccggtctccg ggttcttcgt gaacaccagc ggaaacgtaa taaatcgccc
       61 gccatcctgc ggccaacact tcaatatggg gaattccaaa aggttcacat ccgcgccctt
      121 gcgaatcacg tctttgcaag ccccggattt cacactcttc ggaaaaaacg aacccaactc
      181 cgccagcttc ggcagcattt ttattttgtc gaacaatccc tgcggcgttt ccattttcaa
      241 aaacccctga atcctcgccg ccacgtcctc cagtttgtcc acctcaaacg ccaactccat
      301 ccgccgcacg ctgccaaatg tattaatcag caacggcacc ttcgagcctt taggcttttc
      361 aaaaagtagc gccggtccca cactgtgcgc cgcgcgcgtt ttcgaacgcg ccacccgctg
      421 cgccacttcc gtaatctcca ataccggatc aacctccgcg cgcacgcgct tcagctcccc
      481 ttccttctcc agccgcgcca caaattcccg caaatcccga taccccacac acgcctcccc
      541 atccatcttc caaatcgcaa actgtagcac gaactcatca cgcggttctg ctgccgacgt
      601 tgtttttgag taatgaaaac tatttgacca gcgagaatct cagcgcccgc attacaagtc
      661 gcccgtcaaa acaacggtgg acgttcctcg gcaacgtgaa acgcacgttc taacgcgatc
      721 cctgctcgac ccaaggtgcc ttcctcgaac agtctaccga tcaacgttac gccatgcggc
      781 acgcggcgcg gagtcgagaa ttttggcaac ggatgctgcg ggtccggcgc ccagtccgaa
      841 cgcgcttccg acacttccac aaatcccgcc cgtatcgtga tcgatggatg cccggtgtgg
      901 ttgctgatgg tcaacatctc gtcgcgcagc gaaggcacca acaacacatc gaccttcgaa
      961 aaaatccgcg ccatctcctc agcgacctta cgccgcaagc gatcggcctg cacaaaatca
     1021 actgccgaga gaaaccgtgc ttgccggaaa agattaggcc acgcatccgg tgtctgcact
     1081 ttcaattgat cgagctcatg attcagcgtc agttcttcaa acgccgccgc gccctccgca
     1141 aaaagaatca aattcaacga ctgatatggc caatctggca gagccacttc gaccggaacc
     1201 atccccaact tcggcagcat tgccagaccc gcgcgatcaa cgtccgtagc tggccgttct
     1261 ttcatccacc gcggaaaata cccaactcgc agcccgttca catttgcatt tgcatcaaat
     1321 tgcaggtggc tcgccacgct ggagttgtcc cccgggtccg cgccggaaat cgcatgcagc
     1381 actaacagcg catcttccac acgacgcgtc atcggaccaa gtttgtccag cgaccaacag
     1441 agcgtcatcg ccccgctacg tggcacacgc ccaaaagtag gccgcagccc acaaaccccg
     1501 cagcgcatgc tcgggctcac aatacttcct cccgtttcgc tgccgatcgc gaatcccacc
     1561 aatccggccg cggtagccgc accaggaccc gcgctcgaac ccgacgcgcc ttcttccaac
     1621 aaccacgggt tcatcgtctg cccgccaaac cagatgtcat tcaacgccag tgcaccaagg
     1681 ctaagctttg ccaccaacac ggcgcccgcg tcatctagcc gttttaccac tgcggcatcc
     1741 tctttcggaa ttcgattccg ataaaactcc gctccatacg ttgtcgcgat tcccgccgta
     1801 tccaacaaat ctttcgcccc ccacggaata ccatgcagag gtccgcgata tttgccggcg
     1861 gcaatttctt gatcggcttt tcgcgcctgt tcgagtgcat gctcacgagt aagcgtaatc
     1921 acacagcgca acttgggatc aaaattctcc agccggcgta aataaatttt cgtaagccgc
     1981 tccgacaaaa gcttccgttg ctcaatccag cgcgacagtc gcgtcactgg cgcatacgca
     2041 atatccgcat cgtccgatgg caaggcaccc gcgtccgttt cgccacgcac aaattgatcg
     2101 cgctgcggat ccgccggctg cccgggaaga acagagttgt agcgcgacca tggc
//
LOCUS       DATCBB010000905         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_61033, whole genome shotgun sequence.
ACCESSION   DATCBB010000905 DATCBB010000000
VERSION     DATCBB010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61033"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VII37_09905"
     CDS             complement(<1..477)
                     /locus_tag="VII37_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070096.1"
                     /translation="MWFGSDNFQFAWKKWSTDASLTADISFVGQGGNAHRKAVLMMRQ
                     SLDPDSAYVDVALHGDGLTSLQYRDAKGAPTHEIQSNISAPARLRIEKRGDYVYMWLG
                     QRGEKLQPAGAAIRISFGQPFYVGIGVCSHDKDAIEKAVFSNLELKEVARAAMSDNT"
     gene            complement(846..>2291)
                     /locus_tag="VII37_09910"
     CDS             complement(846..>2291)
                     /locus_tag="VII37_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5070097.1"
                     /translation="TKRGHAKILDFGLAKVTKQPSSSGSDNSLTTMVEDPDQLTTPGM
                     ALGTVAYMSPEQVRAKELDARTDLFSFGVVLYEMASGQLPFRGETSGVIFDGILNRVP
                     ISSVRLNPDLPVALEEIINKALEKDRNLRYQHASEMRSDLSRLQRDTQVRPSPIAGEE
                     IAGLQSQARTANSSKDSSPSTPMPRTLHDSANKELSKNIGAPAVPVAVPVPPPAQQVQ
                     ARVPLPVVASTGSGKRGLYMGLGALVVVGVLFAAGFYLPRRLKIHAGEGKAVFTAKPS
                     ATAPATSQQTAPPASSSGAAPAVAPPSSADGSAVAVPGSVAMPSAQTSVPEKTGPQAD
                     DASNGSAASHVEAAGNIKHSSGGSQETPASAAKRRVAAEHTQAQSEAAAAASAQAQAA
                     AADLKEAETQEDQLNGRAASVSQSLDNLKRRQSAQGYGLRGDVVSAEQRMQSYLSKAQ
                     AALQRQDGPTAKKYFEQADQEISFLEKFLGH"
BASE COUNT          494 a          656 c          599 g          542 t
ORIGIN      
        1 ggtgttatcg gacattgcgg cccgcgcgac ttctttcagc tcaaggtttg aaaatacagc
       61 tttctcgatg gcgtccttgt cgtggctaca caccccaatg cccacataaa aaggctggcc
      121 aaaagaaata cgtatcgcgg ctcccgcagg ctgtaatttt tctccacgtt ggcccaacca
      181 catataaacg tagtcgcccc gtttttcgat gcgcaatcgc gcaggcgctg aaatattcga
      241 ttggatttcg tgcgtgggcg cacccttggc gtcgcgatac tgcagagaag ttagcccatc
      301 gccgtgcagc gctacatcca catacgccga gtctggatcc agactttgcc gcatcatcag
      361 gacagccttg cgatgcgcat tcccaccttg accaacaaat gaaatgtccg ccgtcagcga
      421 tgcatccgtt gaccatttct tccaggcaaa ctgaaaattg tctgagccaa accacatgtt
      481 ttcgccgctg cccgtaagtg tgtagctttg tttagacgag tcatgttcgc tcttacccgg
      541 gtgattgacc gcacccacat caccatgccc ctcaaatatt ccgacagcgg acgagggttc
      601 ctggcaaaac ccaattcgac tcgccgaaaa aaacatcaaa agcaaaaacc ctgccacgaa
      661 tcgcactgct aacttttttc catgcacgat tttttccatt cgcatttgtg ccgcgtagag
      721 tagggcggca tcaggcctct tggttgatta cgcggtgaaa agtaattttg tcaggaagat
      781 tgttgtttgt ggagtattcc atcggcggaa ttaaattgag cggccgaacc gcgtgagctt
      841 cgttctcaat gtcctaggaa tttttctaga aacgaaattt cctggtcagc ttgttcaaaa
      901 tactttttcg ccgttggacc atcttgtcgt tgcagcgcag cttgagcttt tgacagatac
      961 gactgcatac gttgttccgc ggaaacaaca tcaccgcgca acccataccc ctgtgcactc
     1021 tgccgacgct tcaagttatc aaggctttgc gagacggaag ccgcgcggcc gtttaattgg
     1081 tcttcctggg tctcggcttc ttttaggtcc gcggctgcag cctgtgcttg cgcactcgcg
     1141 gcagcagcgg cctccgactg tgcttgggtg tgttcagccg caactcgtcg cttcgcggca
     1201 ctcgccggcg tttcttgcga gccaccagag ctgtgcttga tattccccgc agcctccaca
     1261 tgcgaagcag cagacccgtt cgatgcatca tcagcctgcg ggccggtctt ctcgggcaca
     1321 gacgtttgcg ccgacggcat tgccacactt cctggcacag cgacggccga accgtctgca
     1381 gatgacggcg gggctactgc tggggctgca ccagaagagc tcgctggcgg tgcagtctgt
     1441 tgcgaagtcg ccggtgcggt cgcggagggt tttgcagtaa acacagcttt cccttcgccc
     1501 gcatgtatct ttaatctccg cggcaaataa aatcccgctg caaaaagcac gccaaccacg
     1561 acaagcgcac ccagccccat ataaagcccg cgcttgccgc ttccggtcga agccacgact
     1621 ggaagcggaa ctcgcgcctg gacttgttgt gccggcggcg ggacgggcac cgcgacagga
     1681 actgctggtg cgccaatgtt tttcgacaac tctttatttg cagagtcgtg cagcgtccgc
     1741 ggcatgggag tggatggcga agaatcctta gaggaattag ccgtccgtgc ctggctttgg
     1801 agcccagcta tttcttcacc cgctatcggc gagggcctta cctgcgtgtc gcgctgcaga
     1861 cgagataagt cgctgcgcat ttctgaggcg tgctgatatc ggaggttgcg atccttttcg
     1921 agggccttat taattatttc ctctagcgca acaggaagat cgggattcag gcgcacagat
     1981 gatatcggca cgcgattcaa aataccgtca aaaatgactc ctgaagtttc tccccgaaac
     2041 ggcaattgcc cggaggccat ttcgtaaagc accacgccaa aagaaaaaag atcggtgcgt
     2101 gcatccagct ccttggcacg aacttgttct ggcgacatgt aagcaaccgt gcccagcgcc
     2161 atcccagggg tggtaagttg gtcggggtct tcaaccatgg tggtcagact attgtcgcta
     2221 ccagagctgg atggctgctt ggtcaccttg gcaagtccaa aatcgagaat cttggcatga
     2281 ccacgcttcg t
//
LOCUS       DATCBB010000906         3686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_190010, whole genome shotgun sequence.
ACCESSION   DATCBB010000906 DATCBB010000000
VERSION     DATCBB010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190010"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VII37_09915"
     CDS             <1..996
                     /locus_tag="VII37_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY5070098.1"
                     /translation="GQIDFGEVRGSVRAQTGGGGIRIMYVAGPMEVESSGGSICLTRV
                     AGAVQAATADGTITAWINPSSGPDSGPGGAPNNLPVPVSAHSGTVQLAGASQLASGNG
                     DIVVFLPRNLAASIDALVTKGGEHRIEADPALHLTFQTEGSNSSGPVHATAMLNGGGM
                     PLKLRTSAGKIRLEFLDSDPSLRESRIREQQERLRVVEVAQPTPPTPPAPPQTQEQAD
                     SQRDWLESWLGKLEVAVTGGLREDADDFRNRLTYSPHPVYPELAQRVGIQGVVKLQVR
                     VGKDGRVEVLKLLQGEPALADAAITAVKQWRGKPAWINGKQVEVVSTVTFNFQLN"
     gene            1178..1876
                     /locus_tag="VII37_09920"
     CDS             1178..1876
                     /locus_tag="VII37_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY5070099.1"
                     /translation="MSKDFDQSYPLQPGGSFELQNVNGTVEVEGWDRDAIEIHAVKTA
                     KSKESDLDRVSIEVDAKPRSVSVVTRYPQNAGVEVAVEYVIHVPHGAHVEHVGTVNGT
                     LRIAGVDAIEDLHTVNGNIEVYEGGGSVHAHTTNGNVHLELAHLGATRGEKGEATAET
                     TNGSLLLAVPSDLQANLEARCLNGNFSSELPVALESSLHPREIHGKIGKGGAPIHLHT
                     VNGGIRVVVLRSTV"
     gene            1927..2922
                     /locus_tag="VII37_09925"
     CDS             1927..2922
                     /locus_tag="VII37_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5070100.1"
                     /translation="MNKTMWTIAGGTAVLCTISIVAPGYTAARRSHAAGNQEVMAEPQ
                     VRDFNVVFDEEGGSWLGVETREVTSDNAKGLKLPSERGVVLGRIAPDSPASKSGLREN
                     DVVTELNGQQVEGVMQFRRMIREIPAGRSVQLTVWRDGHTQKINVTLGKSEELRRSMT
                     RVTPGTPGTFAFRMPELPEMPDLPEVAEIPDMPSVDWDGPVLYSGQPRLGIDAEDLAG
                     QLGSFFGAPDGEGILVRSVNSGSPAEKAGVKAGDVITSLNGERIRSVGDLRQKLAAQH
                     SEKDKGAENKDKENNDKSRTVKLGVLRNKSEISLNVELPAPATTTRHLAGHRTHI"
     gene            complement(3009..>3686)
                     /locus_tag="VII37_09930"
     CDS             complement(3009..>3686)
                     /locus_tag="VII37_09930"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5070101.1"
                     /translation="PDKIPGQYVFVRIASPLLVELHTERTMRYFPRGLPIEEFFARRQ
                     SVSVDGHQITALAVEDELILICIHGAKHLWERLSLVADVAAFVARQTSLDWERSLATA
                     RSVGAERMMNTGLVLARNLLGATLPQIVQERVQSDRSALRLATKIASWLSSAGQAPPG
                     LFSRALYRIQMRGSGLSGLGYLLRLTFSPTQEDWSGGAEDNQNGRFAALRRPVRLAKK
                     YRGGEKS"
BASE COUNT          853 a          963 c         1100 g          770 t
ORIGIN      
        1 ggacagattg attttggcga agtgcgcggt tcggtgcgtg cgcagactgg tggcggcggc
       61 atccgaatta tgtatgtagc cgggcccatg gaagtggagt cgagcggcgg cagcatttgt
      121 ctgacgcgcg ttgcgggcgc agtgcaggcg gccaccgcag acggcaccat tactgcttgg
      181 atcaatccct caagcgggcc cgacagtggt cccggtggcg cgcctaacaa tttgcctgtt
      241 cctgtatctg cacattcggg gaccgtacag cttgcggggg cctcgcagct cgcttctgga
      301 aatggcgaca ttgtagtgtt tctgccacgc aaccttgcgg caagcatcga tgcactcgtc
      361 acgaaaggcg gcgaacatcg catcgaagcc gatcccgcgc tgcatttgac ttttcaaacc
      421 gagggctcca atagctcggg tcccgtacat gccacagcta tgttgaatgg cggtggcatg
      481 ccgttgaagc tgcgcacatc cgcggggaaa attcgactgg aattcctgga ttccgatcca
      541 tcgctacggg aatcccggat tcgcgaacag caggaacgct tgcgtgtcgt ggaagtggct
      601 cagccaacgc cacctactcc tccagcgccg ccgcaaaccc aagagcaggc agattcgcag
      661 cgcgattggt tggagagctg gctgggcaag ctggaagttg ccgtcactgg aggcttgcgc
      721 gaggatgctg acgattttcg caaccggctt acctattctc cgcacccggt ttatccggaa
      781 ctcgcgcagc gggttggcat tcaaggcgta gtgaagttgc aggtgcgggt ggggaaagat
      841 ggccgcgtgg aagtattgaa attgctgcag ggcgagccgg cgctagcgga tgcggcaatc
      901 actgcggtga aacagtggcg aggcaaacct gcgtggatca acggcaaaca ggtcgaggtg
      961 gtttccaccg tgacatttaa ttttcagctc aactgattat tcgcgcgtgc agttgacacg
     1021 acattgagat tcgttcgggg tgcaggtcgt aatttcgcgt tacggtccgg gtccgtcgta
     1081 aaaatcgaag ggggtaaaat cgatgcttcg acatgcgttg cgcgcacctc taattctatc
     1141 aatcgcgggt gcggtttgcg ccgcttccgg tttggcgatg agcaaggatt tcgatcagag
     1201 ttacccgttg cagccgggtg gctcatttga attacaaaat gtaaatggca ccgtggaggt
     1261 agaaggctgg gaccgtgatg ctatcgaaat ccacgcagtg aagaccgcca agagcaagga
     1321 gtcggatctc gatcgcgttt cgattgaagt ggacgcgaaa ccgcgctccg tctcggtggt
     1381 cacgcgctat ccccagaatg ccggcgtgga agttgctgtg gaatacgtga ttcacgtgcc
     1441 gcacggagcg catgtagaac atgtgggaac ggtgaacggc acattgcgca tcgccggtgt
     1501 ggatgccatc gaagaccttc ataccgtcaa tggcaatatc gaggtctatg aaggcggcgg
     1561 ctccgtccac gcgcacacca ccaatggcaa tgtacatctg gaactagcgc atctcggcgc
     1621 cacccgcggc gaaaagggtg aagctaccgc cgaaaccact aacgggtcgt tgctgctggc
     1681 cgtgccctca gacttgcagg caaatttgga ggcacgctgc ttgaacggaa atttttcatc
     1741 cgaattgccg gtggcgctcg agagctccct gcatcctcgg gaaatacacg gaaaaattgg
     1801 caaaggcgga gcgcccattc atttgcatac cgtgaacggt ggaattcgcg tggtggtgtt
     1861 gaggtcaacg gtttagcttt tgctacgtct aaactcgcag gagctaatat ttttggagga
     1921 cagatcatga acaaaaccat gtggactatt gccggcggca ccgccgttct ttgtacgatt
     1981 tcaatcgtag cgccgggata taccgcggct cgtcgatcac acgcggctgg aaatcaggaa
     2041 gtgatggccg aaccccaggt acgtgatttc aatgtagtgt tcgacgagga gggtggaagt
     2101 tggctgggcg tcgaaacccg ggaagtcacg agcgacaacg ctaaaggatt aaaactgcca
     2161 agcgagcgag gggtcgtgct gggcagaatt gctccagaca gccccgcttc gaaatctggt
     2221 cttcgagaaa atgacgtggt tacggaactc aatgggcagc aagtggaagg cgtgatgcaa
     2281 ttccggcgga tgattcgcga aattcctgcc ggacgcagcg tgcaactgac tgtgtggcga
     2341 gatggacata cgcaaaaaat caatgtcacg cttggcaagt ccgaggaatt gcgacgcagc
     2401 atgacgagag taacacccgg tacacctggc acattcgcgt ttcgcatgcc ggaattaccg
     2461 gaaatgccgg acctgccgga agtggcggag attcccgaca tgccgtccgt ggactgggac
     2521 ggcccagtcc tttactccgg ccaaccacgc ctgggcattg atgctgaaga cctcgcggga
     2581 cagcttggat cattttttgg agcaccggat ggtgagggaa ttctggtgcg atccgtaaat
     2641 tctggttcgc cggcagagaa agcaggagta aaggccggcg atgtcatcac ttctctgaat
     2701 ggcgagcgca ttcgttccgt gggagacctt cgccaaaagc tcgctgcaca gcatagcgag
     2761 aaagacaaag gcgcggagaa caaggacaaa gagaacaacg acaagtcccg caccgtcaaa
     2821 cttggtgtac tgcgtaacaa gagtgaaatc tctctcaatg tggaattgcc ggcgcctgca
     2881 accacaacaa gacaccttgc cgggcatcgc actcacatct aaataagtca gtaatcagaa
     2941 aagcccggat ttttttatgt ccgggctttt ttgtattccg gcgcgttccc aattcaagca
     3001 ggcatcgttc aggatttttc gccgccacgg tattttttgg caagacgaac aggtcttcgc
     3061 agcgcggcga aacgcccgtt ctgattgtct tctgcgccgc ctgaccaatc ttcctgggtt
     3121 ggcgagaacg taagccggag caggtaacct aatcctgaaa ggccgctccc gcgcatttgt
     3181 atgcgataaa gcgcgcgcga gaataatccc ggcggtgcct ggcccgccga agatagccag
     3241 ctagcaattt tcgtggcaag acgcaaggcc gaacgatctg attgcacccg ttcctgcaca
     3301 atttgcggca atgtagcgcc gagcaaattc cttgctaaaa ccaggccagt attcatcatg
     3361 cgttccgccc cgacacttcg cgcggtggca aggctgcgct cccaatctag agaagtttgg
     3421 cgggctacga acgcggcaac atcagcaacc agcgataaac gctcccaaag atgtttggcg
     3481 ccgtgaatgc aaatcaggat cagctcatcc tctacagcga gagcggtgat ctggtgccca
     3541 tctacgctta cagattgccg ccgtgcgaaa aattcttcta tcggaagccc gcgaggaaag
     3601 taacgcatgg tgcgctcggt atgcagttcg acaaggagcg gcgatgcgat tcgaacgaaa
     3661 acgtattgtc caggaatttt gtcggg
//
LOCUS       DATCBB010000907         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_28907, whole genome shotgun sequence.
ACCESSION   DATCBB010000907 DATCBB010000000
VERSION     DATCBB010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28907"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VII37_09935"
     CDS             complement(<1..637)
                     /locus_tag="VII37_09935"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY5070102.1"
                     /translation="MKKETIHPAKSIAGGVELPGDKSISHRYAMLAGLAEGTSELRHF
                     SAAVDCHSTLACMKALGAEVKIEKDSVKVTGRGARGLKSSWRALDAGNSGTTMRLLAG
                     ILAGQKFTTRLTGDDSLQKRPMKRIVGPLREMGADIRARDDNFAPLEIHGMALKAIDY
                     KMPMASAQVKSAVLLAGLFADGVTTVTEPARTRDHTELALEEFGATVEKHGK"
     gene            complement(947..1276)
                     /locus_tag="VII37_09940"
     CDS             complement(947..1276)
                     /locus_tag="VII37_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY5070103.1"
                     /translation="MKKVAVVLVALVIVMMVVPAAFAQDNAPAGGKLPLNPTTLALAA
                     GFGVAIAAFGCGLGQGRIAAAACEGIARNPGAADGVRGAMILGLVFVETLTLFTFALG
                     FLLYRLL"
     gene            complement(1365..>2135)
                     /gene="atpB"
                     /locus_tag="VII37_09945"
     CDS             complement(1365..>2135)
                     /gene="atpB"
                     /locus_tag="VII37_09945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5070104.1"
                     /translation="FGSFALSLLSALHVKPTDPENPIPEHVIMALAVLVVGTLLALWV
                     RSRLSVERPGASQQIAEILLNNPMGFGIRDVLSQNAGHHWERYIPMVGSIALCVLLGN
                     LFGAFPFLTAPTSAYTVPLACAIITFLYFNWQGIRHHGPIGYMKHFTGPVAWLAPLMF
                     PVEILSTTARLLSLSVRLWANIFASDIIYGLFLGLFAGAFTSGWTKSPILGVVVGVLP
                     ALIPIAFILLHVFVSVVQAYVFTILPAVYIGMATADEH"
BASE COUNT          524 a          670 c          534 g          407 t
ORIGIN      
        1 tttttccgtg tttctcgaca gtggcaccaa attcctcaag cgccagttcc gtatgatctc
       61 gtgtccgggc cggctccgtc accgtggtta cgccgtcggc aaacaatccg gccagcagca
      121 ccgcggactt cacctgcgca ctggccatgg gcattttgta atcaatcgcc tttaatgcca
      181 tgccatgaat ttccagcggc gcaaaattat cgtcgcgcgc ccgaatatcc gcgcccattt
      241 cgcgcaacgg ccccacgatg cgcttcatgg gacgcttctg caaggaatca tcgcccgtca
      301 gccgcgtggt gaacttctgc cccgcaagga tcccagccaa caaacgcata gtcgttccgg
      361 aattccccgc atccaacgca cgccaactgc tcttcagccc ccgcgccccg cgcccggtaa
      421 ctttgaccga gtctttttca atcttcacct ctgcgcccag tgctttcatg cacgcgagcg
      481 tgctgtggca atccaccgct gccgaaaaat ggcgcagctc gctggtgccc tcggccaacc
      541 ccgccagcat agcgtaacga tgcgagatgg acttatcgcc tggcagctcc acaccgccgg
      601 caatagattt agcaggatgt atcgtttctt ttttcacgcg caaattttac cacgctgctc
      661 gcctcgccct tgcattggcg ctcaaccttc agcaaccaag tccgtcgcct tagcaccgca
      721 cttgccgcca aaaccttggc ccacttccgc caccagcaat accggtcatc ccgaccgaag
      781 caggtcggcg attttgcttt tctgcttcgc tcctgcgaag cagccggcct gcgagtggag
      841 ggatctctct tcgcttgtca ttgccgtcaa aacacaaaac aaaaacggct cggaaactct
      901 ctccgagccg ttctcactac caaatgtatc gaggtaacag cgaaaactag agtaggcggt
      961 agagcaaaaa gcccagcgcg aacgtaaaca acgtcagcgt ttctacgaac accagtccga
     1021 gaatcatcgc accacggact ccgtcggctg cgcccggatt gcgggcgatg ccttcgcacg
     1081 ccgctgcggc gatgcgtccc tgccccaaac cgcaaccaaa cgcggcgata gccacgccaa
     1141 atccggcggc caaagccagc gtggtgggat tcagcggaag cttgcctcct gctggagcgt
     1201 tgtcctgcgc gaacgccgca ggaacaacca tcattacaat taccagtgct accagaacca
     1261 ctgctacttt cttcatttac tgcctcctca tgaattgccg ccaggaggtg gttcccggac
     1321 accgtgcagc cgggctcaca ctggagcggc taacaaaatt gttcttagtg ctcgtctgca
     1381 gtggccatgc cgatataaac ggcgggcaga atcgtaaaca cgtaggcctg caccacggaa
     1441 acgaaaacat gcagaagtat gaatgcaatc ggaatcaacg ccggcagtac tccgacgacc
     1501 acgccaagga ttggcgactt ggtccacccc gaagtgaacg ccccagcaaa cagtccaaga
     1561 aataatccat aaataatgtc gctggcaaaa atgttcgccc acagacgaac agaaagagac
     1621 aagagtcgcg cggtggtact gagaatttct accgggaaca tcagcggagc cagccaggcg
     1681 acaggaccag tgaaatgttt catgtaacca atcggcccgt gatgacgaat gccttgccag
     1741 ttgaaataca gaaacgtgat gatcgcgcac gccaacggta cggtatacgc gctcgttggc
     1801 gcggtaagaa acggaaatgc cccaaacaaa ttaccgagta gcacacacag cgctatggaa
     1861 ccaaccatcg ggatgtagcg ttcccagtgg tgcccagcat tttgactgag cacatcgcgg
     1921 atgccgaagc ccatcggatt attcaaaaga atttcggcga tctgttgcga cgcaccaggg
     1981 cgctcgacgg aaagacgcga acgcacccac aaagcgagaa gagtgccgac cactaaaacc
     2041 gcaagcgcca taataacgtg ctcagggatc ggattttccg ggtccgtcgg cttgacgtgt
     2101 agcgccgaca gtaaagacag tgcaaagctg ccgaa
//
LOCUS       DATCBB010000908         1047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_61169, whole genome shotgun sequence.
ACCESSION   DATCBB010000908 DATCBB010000000
VERSION     DATCBB010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61169"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VII37_09950"
     CDS             <1..899
                     /locus_tag="VII37_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070105.1"
                     /translation="GLVTILHLVPGGWPAAQSIAEEQHKFQLVDLSTLLAKPYTLWAG
                     IIGGAFLRTASHGTDQLIVQRLLAARNERQSVLALLSSAVAVFLQFGLFLFVGVMLFA
                     YYRIPSAAFGRADRIYPTFIVSRMPHGISGLLIAAILAAAMSNLSAALNSLSSSTIMD
                     FYVRFRPAVSDPQRLRLARLATLVWALVLFALALLALHRVGRVVEVGLQIASVAYGAL
                     LGVFLLGVLTKRANQRGALVGMLAGLAIEVYLWQWSSVAFTWWVAIGTCMTFTVGYGA
                     SILWKVAPKVAPRQEREGGRET"
BASE COUNT          187 a          272 c          322 g          266 t
ORIGIN      
        1 tagggctcgt gaccatactg catctggtac cgggaggatg gccagcggcc cagtcgattg
       61 cggaggagca gcacaaattc caactggtcg atctttctac tcttctagcg aaaccttaca
      121 cgctttgggc gggcatcatc ggtggcgctt tcctgaggac agccagccat ggcaccgacc
      181 aactcatcgt gcagagattg ctggcggcgc ggaatgaacg gcagtcggtg ctggcactcc
      241 tatcgagcgc cgtcgcggtt tttttgcagt ttggattatt tctttttgta ggagtgatgc
      301 tgtttgccta ctatcgtatt ccgtcggcag cttttgggcg ggcggatcga atctatccaa
      361 cgttcatcgt cagccgcatg ccgcacggaa tttcaggact tttgatcgct gccatcctgg
      421 ctgccgcgat gtcgaacttg agcgcagcat tgaattcgct gtcgtccagt acgattatgg
      481 atttctacgt tcgttttcgc ccggcggtga gcgacccgca acgactgcgg ctggcacgac
      541 tcgcaacgct tgtgtgggcg ctcgtgcttt ttgctcttgc gctgctggca cttcaccggg
      601 tcggacgcgt ggtcgaggtt gggctgcaaa tcgcgtcggt cgcgtatggc gcactgctcg
      661 gcgtgtttct actcggagtt ctaaccaagc gggcgaatca gcgcggcgca cttgttggaa
      721 tgcttgctgg tttggcgata gaagtttatt tatggcaatg gtcatcggta gctttcacct
      781 ggtgggtcgc gatcggaact tgcatgactt ttactgttgg ctacggcgcg agcatcttgt
      841 ggaaggttgc cccgaaagtt gcacctcgcc aggagcgcga gggtgggaga gaaacatgaa
      901 aagcagaatt gctttgcgag gaacggggaa cgaattctca tgcctgtaga cgacggccat
      961 cttcgcccgg aactggcgcg cgatctcgga ctctcccatg ccggtgccgt cgtcgtcggg
     1021 acaatcatag gtagcgggat ctttttg
//
LOCUS       DATCBB010000909         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_224196, whole genome shotgun sequence.
ACCESSION   DATCBB010000909 DATCBB010000000
VERSION     DATCBB010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224196"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..460)
                     /locus_tag="VII37_09955"
     CDS             complement(203..460)
                     /locus_tag="VII37_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5070106.1"
                     /translation="MPIAHDKTLTIFEWFFHDVSDSKVQERIQRAIAFSDQVQQEDIG
                     LCESVQRGLRSATYDRGRYSVKRENGVHHFHMLLGEFLGRP"
     gene            complement(463..1335)
                     /locus_tag="VII37_09960"
     CDS             complement(463..1335)
                     /locus_tag="VII37_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5070107.1"
                     /translation="MNSKPLQLDVNEQIECAHTLASRFYTDPAILDVEKTRIVLRTWQ
                     LVGTLNHPCGEVDGVKRSIADPESFFTADVAGEPIIVVRDKQGVLRAFSNVCRHRAGP
                     IALGSGCKNVLRCQYHGWTYTLDGRLIGTPDVEGVEFFDRSTMGMVPLRVEVWEQFIF
                     VNFDAQAEPLAAYLGKIPEQVHGFQFEGLQFAERRDYVINCNWKVYVDNYLEGYHIPI
                     AHPGLMREIDYAHYRTDTHRYYSQQFAPIRAMKAEENAERFYAPGSGLQQAAYFWIFP
                     NLMVNIYPTMFQPT"
     gene            complement(1349..>2047)
                     /locus_tag="VII37_09965"
     CDS             complement(1349..>2047)
                     /locus_tag="VII37_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5070108.1"
                     /translation="PDADMELATQIIGDSAFGCAGQRCLAVSVAVTIGDAQKTFRDSI
                     SHAASSLRVGNGLGADVQMGPVISKESKQRVESLIAAGVAEGAKPIVDGRNTKIANAE
                     GGNFIKPTILDGVPASSKLTHTEIFGPVLSLVHADSIDDAMKFLHNSPYGNQASLFTT
                     NGAAARKFRYEAPAGNIGINIGVAAPMAYFPFSGWKDSFFGILHGQGRDAVEFYTESK
                     IVIERWSRQETRKF"
BASE COUNT          468 a          585 c          518 g          476 t
ORIGIN      
        1 ctgaagtttt gattggcgga aaccacgcag aagtagcggc gtggcggagt gcggcggcaa
       61 tcgaaaagac tcagaaaaac cggcccgatc ttttcattcg ttaattccac gtgcgaggat
      121 agttcgcgaa ttagaatgac cctgtattcg ggactgcgtc gcacctttat tcttgtttgc
      181 gaatgagcac gcttggcgac agctaagggc ggccgaggaa ctcgccgagc agcatgtgga
      241 aatggtgcac gccattttcc cgctttaccg agtagcgccc gcgatcgtag gtcgcggaac
      301 gaagaccacg ctgcacactt tcgcacagcc ctatatcttc ttgctgcacc tgatcactaa
      361 aagcaatggc gcgctggatg cgctcctgca ctttgctatc gctcacgtcg tgaaaaaacc
      421 actcaaaaat ggtcagcgtc ttgtcgtgcg caatcggcac aatcaggttg gttgaaacat
      481 tgtcggataa atgttgacca tcaaattggg gaaaatccag aaatacgcag cttgttgcaa
      541 cccgctgccc ggcgcataaa accgctctgc gttttcttcc gccttcattg cgcgaatggg
      601 cgcgaattgt tgcgagtaat agcgatgcgt atcggtgcga tagtgcgcgt aatcaatctc
      661 gcgcatcaat cccggatgag cgatgggaat atggtagccc tcgaggtaat tatcgacgta
      721 caccttccag ttgcagttga tgacgtaatc tcgtcgctcc gcgaattgca gaccttcaaa
      781 ttgaaatccg tgaacctgct cgggaatctt tcccagatac gccgctagtg gctctgcttg
      841 cgcatcgaaa ttcacaaaaa tgaattgttc ccagacctcc acccgcagcg gcaccatgcc
      901 catggtgcta cggtcaaaaa attccacgcc ttcaacatcc ggcgtgccaa tcaagcggcc
      961 gtcgagcgta taagtccagc cgtgatactg gcagcgcaac acgtttttgc agccgctgcc
     1021 taatgcaatc ggcccggcac ggtggcggca aacattactg aaggcccgca aaactccctg
     1081 cttgtcgcgc accacaatga tgggctcgcc ggccacgtca gcagtaaaaa agctctcagg
     1141 atcagcgatg ctgcgcttta caccgtccac ttcgccgcat ggatggttaa gtgtgccgac
     1201 gagttgccag gtccgcaaaa cgattcgcgt cttttcgaca tctaggatcg cgggatcagt
     1261 ataaaacctg gacgcgagcg tgtgcgcaca ctcgatctgc tcgtttacgt cgagttgcag
     1321 cggcttgcta ttcattcttg cggccgcatt agaattttct ggtttcctgc cttgaccagc
     1381 gctcgatcac tattttcgat tcggtataga actccaccgc gtcgcggccc tgtccatgca
     1441 aaatcccgaa gaagctatct ttccacccgc taaacgggaa gtacgccatc ggtgcggcca
     1501 cgccaatatt gatgccaata tttcctgccg gtgcctcgta gcgaaacttt cttgcggccg
     1561 cgccattggt ggtaaaaagc gaggcctggt ttccataagg actgttgtgc agaaatttca
     1621 tcgcgtcatc aatggaatcc gcatgcacca ggctcaacac cggtccaaaa atttccgtat
     1681 gcgttaattt gctggaagca ggcacgccat caagaatggt gggcttgata aaattgcctc
     1741 cttcggcgtt ggcgattttt gtgttgcgtc cgtcgacaat aggcttggct ccctcggcga
     1801 cgccagccgc aatcaacgac tccacacgtt gcttactttc cttgctgatc accggcccca
     1861 tctgcacatc ggcgcccagc ccattaccta ctcgcagcga agatgccgca tggctgatcg
     1921 aatcgcgaaa agttttctgc gcgtcgccaa tcgtgaccgc cacggaaaca gcaaggcagc
     1981 gttgtccagc gcagccaaac gcgctgtccc cgatgatttg cgttgccaat tccatatccg
     2041 catctgg
//
LOCUS       DATCBB010000910         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_287780, whole genome shotgun sequence.
ACCESSION   DATCBB010000910 DATCBB010000000
VERSION     DATCBB010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287780"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1454)
                     /gene="tgt"
                     /locus_tag="VII37_09970"
     CDS             complement(318..1454)
                     /gene="tgt"
                     /locus_tag="VII37_09970"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY5070109.1"
                     /translation="MNFSFEVNKTDATGARRGRLTTPHGTIETPVFMPVGTAATIKAL
                     THETLEELGATIILANTYHLYLRPGHELIRKLGGLHKFMSWQRAILTDSGGYQVFSLS
                     ALRKITDAGVTFRSHLDGSEHLLTPEKAVEVQLALGSDIAMVLDECIETPAPREKAEA
                     ALNRTTRWAKRARQHFLAQENSYPGVRQWQFGIVQGATFADLRRESARQLLDMDFAGY
                     AVGGLAVGEPHNMTCEMTGEVTALLPADRPRYLMGVGKPEQLADYVALGIDMMDCVLP
                     TRAARHACLYTSEGRVLIKNARYAADTRPIDPQCSCSVCKRYTRAYLRHLFAAGEITA
                     SILATHHNIHFYLDIMRQIREAIEFGNLAKFSAEMQACYAAGPA"
     gene            complement(1480..>2072)
                     /locus_tag="VII37_09975"
     CDS             complement(1480..>2072)
                     /locus_tag="VII37_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEY5070110.1"
                     /translation="LSIIVSGLLAQKTDWRNFFERQMSKNARLDQMPQDQKDRMLENQ
                     VKYAPTFALVFGTIVTPIFVLLVTLAYWGAFNLFNGAALSFKTAFGIVSHAFVPLVIS
                     SLLATIILLIKPRGDVDPEHFLASNVAAFLPDDTPKWLEALGQSLELFWIWTLALVAI
                     GFSAANPKKIKPAGAFLTTFGIWAIWVLGKVAWNMF"
BASE COUNT          470 a          584 c          565 g          453 t
ORIGIN      
        1 cgaacatcaa gcccaacggg atcaggaaac tggaaaatcc cgaagccggg cttgcctgaa
       61 aaaacgtggc caaaatcatt tgatctgtga aaaccgatcc cttcttttac cggcaaagcc
      121 cctggttcag cccagaaatt cagccctgaa acggcatcca gcatacctca aaaaaacagg
      181 gaaagattgt cctggggcaa gttccccgcg tccatccccg ccattatctt tggatattag
      241 cggccgcgat gcgattaccc gcggtcatgt tcggcgccct gaattacgcg ctcccaataa
      301 gcgcctcggt tcaccactca ggccggaccg gccgcgtaac atgcctgcat ctctgctgag
      361 aattttgcga ggttcccgaa ctcgatagcc tcgcggattt gccgcatgat gtcaaggtaa
      421 aagtggatat tgtggtgggt cgccaggatc gatgcggtga tttctcccgc ggcaaaaaga
      481 tggcggagat acgcgcgcgt gtaacgtttg caaacgctgc agctgcactg cggatcgatc
      541 gggcgagtgt ccgcagcgta gcgcgcattt ttgatcagca cccggccttc gctggtgtac
      601 agacaggcgt gccgcgcggc gcgtgtgggt aacacgcagt ccatcatatc gatgcccaga
      661 gccacgtagt cagcgagctg ctccggtttg cccacgccca tcaaatatct cggacggtcc
      721 gcgggcagca aggcggtgac ctcgccagtc atttcgcatg tcatgttatg aggttcgccg
      781 acagccaggc cgcccaccgc gtagccggca aaatccatgt cgagcagttg tcgcgcgctc
      841 tcgcgtctta aatcagcaaa tgtcgcgcct tgaacgattc cgaattgcca ctgccttacg
      901 cctggatatg agttttcctg cgccaaaaaa tgctgccgcg ctcgtttggc ccaacgcgtc
      961 gttcgattta gcgcggcttc cgctttttct cgcggcgcgg gtgtttcgat gcactcatcc
     1021 agcaccatgg caatgtccga gcccaatgcg agctgaactt cgacagcttt ttctggagtc
     1081 agtaagtgtt cggagccgtc caggtgagat cgaaaggtga cccctgcgtc ggtgattttt
     1141 cgcagcgcgg acagactgaa tacctggtaa ccgccagagt ccgtcagaat tgcccgttgc
     1201 caggacataa atttgtgcag gccgcccaac tttcgaatca gttcatgtcc aggccgaaga
     1261 tatagatgat aggtgttagc gaggatgatg gtcgcgccca actcttccag cgtttcatgc
     1321 gtcagagctt taatggttgc ggcggttccc actggcatga acaccggggt ttctatggtt
     1381 ccatgcggag tggtgaggcg tccgcggcgg gcgcctgtgg catctgtctt atttacttca
     1441 aacgaaaaat tcactaaggc agtttagcag tagccgctgt taaaacatgt tccaggccac
     1501 tttgcctaaa acccaaatgg cccagatgcc gaaggtcgtg aggaacgcgc ccgccggttt
     1561 aattttcttg gggtttgcag cggaaaaacc gatggccacc agcgccaggg tccatatcca
     1621 gaatagctcc agcgattgcc ccaacgcttc aagccacttc ggcgtgtcgt ccggcaaaaa
     1681 ggccgcaacg ttgctggcga gaaaatgttc ggggtccaca tcgccgcgag gtttaatcag
     1741 cagaatgatt gttgccaaaa ggctgctgat gaccaacgga acgaaagcgt gcgaaacgat
     1801 gccgaaagcg gtcttgaagc tcagcgccgc tccgttgaaa agattgaagg ctccccaata
     1861 cgccagcgtc accagcagaa caaagatcgg cgtgacgatc gtgccaaaga ccaacgcgaa
     1921 cgtaggcgcg tacttcacct ggttctccaa catgcgatcc ttttggtctt gcggcatttg
     1981 atcgagccgc gcattcttgc tcatctggcg ctcaaaaaaa ttgcgccagt ccgttttttg
     2041 ggcgaggagt ccggaaacaa taatgctcaa ca
//
LOCUS       DATCBB010000911         2675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_484536, whole genome shotgun sequence.
ACCESSION   DATCBB010000911 DATCBB010000000
VERSION     DATCBB010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484536"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VII37_09980"
     CDS             complement(<1..323)
                     /locus_tag="VII37_09980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5070111.1"
                     /translation="MHNASLLNGPRFSVIITCHNQSAYIRDAVNSALEQGCESKEIVV
                     VDDASSDASPQLLKEYGDKIHFAAFQTNQGCTTSRNLGAAMATGEYFVFLDGDDVLLP
                     WALDV"
     gene            complement(450..1772)
                     /locus_tag="VII37_09985"
     CDS             complement(450..1772)
                     /locus_tag="VII37_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY5070112.1"
                     /translation="MADSIPSVQEGLPGESVPGPASPAPASRFGQAAFIFIFITILLD
                     FLALGIIAPVLPNLIIQFKGGNIASAASITGYFGFAWAAMQFIFSPILGAWSDRFGRR
                     PIILISCAGLGFDYILMALAPTLSWLFVGRLISGITTSNISTAFAYVTDVTPPERRAK
                     QFGLISAAFGLGFIVGPAVGGLLGNVHLRLPFWIAAGLSLTNAIYGFFILPESLPPER
                     RAKKAWHMANPLGALTLLRSHPELGGLAIVTVLYYLAHQVLPTIFVLYADYRYGWSER
                     TIGLSLAAVGVCVSLASGLLVGPFVKRFGERNSLLSGLTFGFLAFMGFALASRGWMAF
                     ASIPFIALWGIAGPAMQSLMSQRVDPTSQGKLQGAINSLRAITGMIGPLLFTQVFAIA
                     ISPRVRVHVPGAPYYMAALLMLASASVAYIVGRPALPVTAPQTADGNS"
     gene            complement(1791..>2675)
                     /locus_tag="VII37_09990"
     CDS             complement(1791..>2675)
                     /locus_tag="VII37_09990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY5070113.1"
                     /translation="NVEIAHSLECLRIPDGMVVRPQDIRGFDPKKIVILASGSQAEPM
                     SSLSRIAVDNHRFVSVDENDSVILSARIIPGNEKAIFRMLDHMFRRRALVYYDNSAGT
                     IHVSGHASQEEQKLLLQLVKPKYFIPVHGEYRHLFRHAALAHQIGCVSGEILLLEDGK
                     TIEFTEDGVHRRDPVTAGRVCVDSGSLEEIEEVVIRDRKHLSEDGVVVSIIAIDKHTG
                     KMESHPEIVTRGLMSDNGQELIAGARAVIINTVEQSNAEEKSDWGVIKEKIRVDLKRY
                     INKHTSKRPLILPVILEV"
BASE COUNT          678 a          748 c          699 g          550 t
ORIGIN      
        1 taaacatcca acgcccatgg cagcagcacg tcatcgccat caaggaatac aaaatattca
       61 ccggtggcca tggccgcgcc cagattgcga gaagtggtac agccctggtt ggtctgaaat
      121 gcggcgaaat ggatcttatc gccgtactct ttcaggagtt ggggcgaagc atcgctggac
      181 gcgtcgtcca ccactacaat ttctttcgat tcgcagcctt gttccaacgc ggagttcacc
      241 gcgtcccgga tatacgcact ctgattatgg cacgtgataa ttacgctgaa gcgcggccca
      301 ttcagtaggc ttgcgttgtg catgcgggaa gtctagcacc gggtcaagaa gagaaagcgg
      361 tatctgtaca cggtgttact tttttgcaac cccgtacccc gttgataccg atttcgccgc
      421 ccgacagtcg aaccgctaaa tatcagccgt taggagttgc cgtcggcggt ttgcggtgca
      481 gtcaccggca atgccggacg ccccacaatg tacgccacgc tcgcgctggc cagcattaac
      541 aacgcggcca tataatacgg cgctcctggc acatgcactc gcacacgcgg agaaattgcg
      601 attgcgaaaa cctgcgtaaa tagcaacgga ccaatcatcc cggtgatagc acgcaaacta
      661 ttgatggcac cttgtagttt tccctgtgaa gtgggatcca ctcgttgcga catcagtgat
      721 tgcattgctg gcccggcgat gccccataaa gcgatgaaag ggatggacgc gaacgccatc
      781 catccccgcg acgccaatgc aaatcccatg aaagctagaa agccaaatgt caaaccggac
      841 agcaaactgt ttcgctctcc gaaacgtttc acaaatgggc cgacgagcag tccagacgca
      901 aggcttacgc acacgcccac ggccgcaagc gaaagtccga tggtgcgctc gctccatccg
      961 tagcggtaat cggcatacaa cacgaaaatc gtgggcagca cctggtgcgc caaataataa
     1021 agcaccgtaa caatagccaa gccgcccaat tccgggtgcg aacgcaaaag cgtcaaagca
     1081 ccgaggggat tcgccatgtg ccacgctttt ttcgcacggc gctcgggcgg aagcgactcc
     1141 ggcaaaatga aaaagccata aatcgcgttc gttaggctta gtccggcagc gatccagaag
     1201 ggaagtcgca aatgcacgtt acctaatagc ccgccgaccg ctggcccaac aatgaatcca
     1261 agtccaaagg ctgcgctgat taatccgaac tgtttggcgc gacgctcggg tggagtgacg
     1321 tccgtaacat acgcaaatgc cgtggaaatg ttagaagtgg tgattccgga aatgagcctg
     1381 cccacaaaca gccagctcaa ggtcggcgcc aacgccatta aaatgtaatc gaatcccaat
     1441 ccggcgcaag aaatgagaat gatggggcgc cgtccaaaac gatccgacca tgcgcccaat
     1501 atgggagaaa aaataaattg catggccgcc catgcgaatc cgaaatatcc agtgatactg
     1561 gcagcactcg caatgttgcc gcctttgaat tgaatgatca ggttgggcag gacgggagca
     1621 attatgccta gggccagaaa atccaacaga atggtgataa aaataaaaat aaatgcagct
     1681 tgcccaaaac gcgatgcagg agcaggcgag gccggaccgg gcacactctc gccaggtaaa
     1741 ccttcttgaa cggatggaat cgaatcagcc attaaaaaaa tactgctgcg ctagacttcc
     1801 agaatcaccg gcaaaataag cggccgcttg gacgtgtgtt tattaatgta acgcttcagg
     1861 tccacacgaa ttttttcctt gatgacgccc caatcggatt tttcttccgc gttggattgc
     1921 tcaacggtgt taatgatcac cgcgcgggcg ccggcaatta attcctggcc gttgtcgctc
     1981 atcaatccgc gagtgacgat ttccggatga gattccattt ttccggtgtg cttgtcgatg
     2041 gcgatgatgg aaaccaccac gccgtcctcg gaaagatgct tgcggtcgcg tatcaccact
     2101 tcttcgattt cttccagcga gccggagtcc acgcacaccc gtccggcggt cacaggatcg
     2161 cgacggtgca cgccatcttc ggtgaattcg atagtcttgc catcttccag caatagaatc
     2221 tcgcccgaaa cgcacccgat ctggtgcgcc agagccgcgt gcctgaaaag atgccggtat
     2281 tcgccgtgca ccgggatgaa gtattttggc tttaccagtt gcagcaagag cttttgttct
     2341 tcctgactgg catgcccgga gacgtgaatc gtgccggcgg aattatcgta gtaaaccagc
     2401 gcgcgcctgc ggaacatatg atccagcatg cgaaaaattg ctttttcatt gccagggatg
     2461 atgcgcgcgg aaagaatgac cgaatcgttt tcgtccaccg aaacaaagcg gtgattgtcc
     2521 accgcgatgc gcgacagcga tgacattggc tctgcttggc tgccgctggc cagaatcaca
     2581 atcttcttgg gatcgaaccc gcgaatgtcc tgcggccgca ccaccatgcc gtccggaatc
     2641 cgcaagcact ccagcgagtg cgcgatttcc acgtt
//
LOCUS       DATCBB010000912         3179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_484538, whole genome shotgun sequence.
ACCESSION   DATCBB010000912 DATCBB010000000
VERSION     DATCBB010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484538"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VII37_09995"
     CDS             complement(<1..509)
                     /locus_tag="VII37_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070114.1"
                     /translation="MPLTILRYSEQTNMTVPEFANSSKRVPPIRIRNCNPAPVRGSSS
                     YVLYWMIANRRLTYHFALDRALEYCRELRKPLVIFEALRCGYQWASDRFHRFVIDGMA
                     ENAGACERHRIQYYPYVEPKPGAGKGLLEAFAADACVVITDEFPCFFLPRMVAAAGRK
                     LPIRLEAIDS"
     gene            581..1927
                     /locus_tag="VII37_10000"
     CDS             581..1927
                     /locus_tag="VII37_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5070115.1"
                     /translation="MKRITIELFLVVCISAANARAQVQKPESKAPETKTVVLKAARLF
                     DGKSNSLTTPGLVIVTNGKISATGANANVPAGAEVIDLGDATLLPGFIDAHTHLTMMY
                     TTDYKQAALDGLQKTIAEQTLDSIANAKVTLMAGFTTVRDVGSHDFIDVGLRNAIRNG
                     KVPGPRMLVSVHAIGATGGHCDETGYRSGVFGKETGPEQGVANGPDQVRYAVRLAHKY
                     GADIIKTCASGGVLSLADDVDTPQLTQEEMNALIDEAHALKRKVAAHAHGAEAAKRAI
                     RAGVDSIEHGSFLDDEALDMMKQHGTYYIPTLMALQGLSEQMQAGMYIPPAIRAKAEQ
                     AMTSLHKTFQRALAKNIKIGLGTDAAVYPHGRNGEEFHQMVDLGMKPIDALRAGTSVD
                     AELLGLADKIGSLEAGKSADVVAVPGNPLQDIRQTEHVLFVMKEGVIYKNERPEAH"
     gene            1990..>3179
                     /locus_tag="VII37_10005"
     CDS             1990..>3179
                     /locus_tag="VII37_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070116.1"
                     /translation="MTVSSEEVNASNLSANTKRVWLFALLVSLATALAIIFPFFWMGN
                     ASGHDFSFHVASWMDAAAQWREGILLPRWTDGANHGFGEPRFIFYPPISWMFGAALGL
                     VVPWIFVPAVFIVLIQTLAGLCAFVLGKRLFPQRGALVCAVCYAANPYALLIVYMRSD
                     FAEQLALAFLPLLFLAAAGASGVLDSPVSSLRRSIVFLAITFAAVWLMNAPAGVVASY
                     SLAMLFAWAAVTQKSWRPLLHGAAALALGFGIAAFYLLPAAYEQKWVNISLALASGLQ
                     PSQNFLYAVIADAEHNAFNRIASNTAVLMIVLTAAFAALSFQRIRSGSRPITKSLWSA
                     LVALSVMAAFLMMPISNVFWIALPKLRFVQFPWRWMFILAIPLACFISAAAVHKRMRG
                     YRAPL"
BASE COUNT          704 a          832 c          896 g          747 t
ORIGIN      
        1 gagtctatgg cttcgaggcg gatgggcaac ttcctgcctg cggcggcaac catccggggt
       61 agaaagaaac acggaaattc gtcggtaatt acgacacagg catccgcagc gaatgcttcc
      121 agaaggcctt tgcccgctcc gggcttcggt tcgacgtaag gatagtattg gatgcgatgc
      181 ctctcgcagg cgcccgcatt ttcggccatg ccatcaatga cgaagcggtg aaaccggtca
      241 ctggcccact gatacccgca gcgaagagct tcaaaaatta ccaatggctt gcgcagttcg
      301 cggcagtact ccaaagcgcg atccagcgcg aagtggtaag taaggcggcg attcgcaatc
      361 atccaataca acacgtagct gctggagcct cgcacagggg cgggattgca attccggatg
      421 cgaatgggcg gaaccctctt tgaggaattt gcaaattcag gaacggtcat gttggtttgt
      481 tcagaataac ggaggatcgt aagcggcacg atacccggag tccttgacat ttgctggaac
      541 caagttatcg tctgcgtctg gccgtactga ggagataaga gtgaagcgca tcacaatcga
      601 actatttctg gtcgtatgca tttccgcggc gaacgctcgc gcacaagtac agaaacctga
      661 aagcaaggct cccgaaacaa agaccgtagt gcttaaggcg gcgaggctat tcgatggcaa
      721 gagcaattcg ttgacgacgc cgggcctggt gattgtgacc aacggaaaaa tttccgccac
      781 aggtgcgaat gccaacgtgc cagcaggagc ggaagtaatc gatctgggag atgcaaccct
      841 gttgcctgga ttcatagatg cgcatacgca tctaaccatg atgtacacca cggactacaa
      901 gcaggcggct ctggacggac tgcaaaaaac tattgccgaa caaaccctgg attccatcgc
      961 aaacgcgaag gttaccttaa tggccggatt tacaactgtg cgcgatgtcg gctctcacga
     1021 tttcattgat gttggcctac gcaacgctat tcgcaatggc aaagttcccg ggccgcgcat
     1081 gctggtcagt gtgcacgcca ttggagcaac cgggggccac tgcgacgaaa ccggctatcg
     1141 ctccggcgtg tttggaaagg aaactggacc ggaacaaggt gtggctaacg gcccagacca
     1201 ggtgcgttac gcggtgcgcc tggcgcacaa atacggcgct gacattatta aaacctgcgc
     1261 ttcgggcgga gtactttctc tggccgacga tgtggacacg ccgcaactga cccaggaaga
     1321 aatgaatgcg ctgatcgatg aggcccacgc gttaaaaaga aaagtagccg cccacgcgca
     1381 tggagcggaa gccgccaagc gcgccattcg cgctggagtc gattccatcg agcatggatc
     1441 attcctggat gacgaagccc tggacatgat gaaacaacac ggcacctact acatcccgac
     1501 attgatggct ttgcagggtt tgtcggagca gatgcaggct ggcatgtata ttcctcccgc
     1561 gattcgggca aaagccgagc aggccatgac ctctctgcat aagacttttc aaagagctct
     1621 ggctaaaaat attaaaattg gactcggcac ggatgcggcg gtctatccgc acggacgcaa
     1681 tggcgaagag tttcatcaga tggtcgatct gggcatgaag cccatcgacg ccttgagagc
     1741 cggtacatcc gttgatgccg aattgctcgg attggctgac aagattggtt ctctggaagc
     1801 cggcaagtcg gctgatgtcg ttgccgttcc cggtaatccg ctgcaggata ttcgccagac
     1861 agagcatgtg cttttcgtaa tgaaagaggg cgtgatttat aagaacgagc gaccagaagc
     1921 gcactgacgc gatcgcgcct tgcatttagt tagttacgac tattttcacg gaccatcaat
     1981 gaacctcgca tgacggtttc ttccgaagaa gttaacgctt caaatttaag cgcaaatacc
     2041 aagcgagtct ggctgtttgc gttgctcgtt tccctggcca ccgccctggc aattattttc
     2101 ccgttctttt ggatgggaaa cgcttcaggt cacgatttct cttttcacgt ggcgtcctgg
     2161 atggatgcag cagctcagtg gcgcgaagga atcctgctgc cacgatggac tgacggagcg
     2221 aaccacggat tcggcgagcc gcgatttatt ttctatcctc ctatttcctg gatgttcggc
     2281 gctgcgctcg ggttagtggt gccgtggatt tttgtgccgg cagtgttcat cgttttaatc
     2341 caaaccctgg cgggattgtg cgccttcgta ctagggaagc gtttattccc gcaacgtggc
     2401 gcgctggtgt gcgcggtttg ttatgcggcg aatccctacg cgttgctcat cgtgtacatg
     2461 cgcagcgatt ttgcggaaca actcgcactg gcttttcttc cgctgctgtt tttggccgca
     2521 gcaggagcct cgggagtctt ggactcacct gtttcctcac ttcggcgttc catcgtattt
     2581 ctggccatta cgttcgcggc ggtttggctc atgaacgctc ccgccggcgt ggtggccagc
     2641 tatagcttgg ccatgctatt tgcgtgggca gcggttacac aaaaatcgtg gcgcccactg
     2701 ttgcacgggg ccgcggcgct ggcgttgggt tttggcatag cagcgtttta tctcttgccc
     2761 gcggcctacg aacaaaagtg ggtgaacatt tccctggcgc tggcgtccgg tttgcaaccc
     2821 tcgcagaatt ttttgtacgc cgtaattgcc gacgcagaac acaacgcttt taatcgtatc
     2881 gcctccaaca ccgcggtctt gatgatcgtg ttgaccgccg catttgccgc gttgagtttt
     2941 caacgtataa ggtcaggttc acggccgatt acgaaaagtc tatggagcgc attggtggcc
     3001 ctttcggtga tggcggcatt tttgatgatg ccgataagca atgttttctg gatcgcgctt
     3061 cctaaattgc gtttcgtaca attcccctgg cggtggatgt tcattctggc cattccgctt
     3121 gcgtgtttta tctccgcagc ggcagttcac aaacgaatgc gcggatatag agcgccact
//
LOCUS       DATCBB010000913         1511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_224225, whole genome shotgun sequence.
ACCESSION   DATCBB010000913 DATCBB010000000
VERSION     DATCBB010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224225"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VII37_10010"
     CDS             complement(<1..1280)
                     /locus_tag="VII37_10010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5070117.1"
                     /translation="MTLACSGDAVIISDSAHRVTHLNAPAELLTGWPQAEARGHALNE
                     ILGETACVDPLAAACGAHEDSPCADTAILRRRDGVELNIKNCSGYIRDRDGNVTGTVV
                     VLHDLGAVRAQILELSQAAQHDFLTGLPNRSLVNDRITQSISFSARYNKQLAVMFVDL
                     DLFKRINDSLGHAVGDKLLQQVAIRIVACVRRSDTVARIGGDEFVVVLSQVGHAEDAV
                     SIARKILTSLAAPYLIDQKQFQINASIGVSTYPDDGTDSETLVHRADTAMYEAKKLGR
                     NNCQFFRPEMQARVLEWQSLEGSLRCALERHEFMLEYQPKIDLATGEISGVEALLRWN
                     HPDRGVIQPLKFVPIAEESGLIVPIGQWVLLEACRQARAWMDAGLPPIRIAVNVSALQ
                     FAAKDFLSSVHAALISTGMDPHNLELELTETVLM"
     gene            1293..>1511
                     /locus_tag="VII37_10015"
     CDS             1293..>1511
                     /locus_tag="VII37_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070118.1"
                     /translation="MLEKRFFFIGVPLDHEVHRPAQAEMIQIAFQEVILRAAVHRLLG
                     DIFVFHAAEYQDRNVRGGSQQPFKGGDTP"
BASE COUNT          331 a          415 c          434 g          331 t
ORIGIN      
        1 tgcatcaaga ccgtctcggt caattctagt tcgagattgt gagggtccat accggtggag
       61 attagggcag cgtgaacgct agacaaaaaa tcttttgcgg cgaactgcaa ggccgacacg
      121 ttgacggcga tgcgaattgg cggcaaacca gcgtccatcc aggctcgcgc ttgccggcaa
      181 gcctccagca gcacccactg accaatgggc acaatcaagc cggactcttc ggcaatgggc
      241 acaaatttta acggttgaat aacgccgcga tcgggatgat tccaacgcaa cagcgcttct
      301 acgccgctga tctctccggt agcgagatcg attttcggct ggtactccag catgaattcg
      361 tggcgttcga gtgcgcagcg caagctgccc tccaaactct gccattccaa aacccgtgcc
      421 tgcatttcgg ggcggaagaa ttgacagtta ttgcgcccca gttttttggc ttcatacatg
      481 gcggtgtctg cgcggtgaac cagtgtttcg gagtcggtgc cgtcgtcagg ataggtgctg
      541 accccgatgc tggcgttgat ctgaaattgc ttttgatcga tcagataagg agcggccaaa
      601 gaggtcaaaa tttttctggc aatagaaacg gcatcttcgg cgtggccgac ttgagaaagc
      661 accaccacga attcatctcc gccgatgcgg gccacggtgt ctgaacgacg cacgcaggcc
      721 acgatgcgga tagccacctg ctgcaataat ttgtcaccga cggcatgtcc gagggagtcg
      781 ttgattcttt taaagagatc gaggtccaca aacattaccg ccaactgttt gttatagcgc
      841 gcggagaagg agatggactg ggttatacgg tcattgacaa gtgagcgatt gggaaggccg
      901 gtcaaaaaat cgtgctgtgc cgcctgggaa agctcgagga tctgcgctcg cacagcgccc
      961 aagtcgtgca ggaccaccac ggttccggtg acgtttccat cccggtcacg gatgtacccc
     1021 gagcaatttt ttatattcag ctcgacgccg tcacgccgcc gcaatattgc ggtgtcggcg
     1081 caaggtgagt cttcgtgtgc gccacaggca gcggccagcg ggtcaacaca agcggtctct
     1141 cccaaaattt cgttcagtgc atggccgcgc gcttctgctt ggggccagcc ggtaagcagt
     1201 tccgctgggg cgttcaggtg cgtcacccgg tgggcgctat cgctaatgat gaccgcatcg
     1261 ccagagcacg ctaaggtcat ttccgcgcaa cgttgttgga aaaacgattt ttcttcattg
     1321 gcgtacctct ggatcatgag gtgcaccgcc ctgcgcaagc ggaaatgatc caaattgcgt
     1381 ttcaggaggt aatcttgcgc gccgcggtcc accgcctgct tggcgacatt ttcgttttcc
     1441 acgccgctga gtatcaggat aggaatgtgc ggggcggcag ccaacaacct ttcaaaggcg
     1501 gcgacacccc g
//
LOCUS       DATCBB010000914         3634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_287866, whole genome shotgun sequence.
ACCESSION   DATCBB010000914 DATCBB010000000
VERSION     DATCBB010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287866"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2547
                     /locus_tag="VII37_10020"
     CDS             <1..2547
                     /locus_tag="VII37_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5070119.1"
                     /translation="NQVGSPQNPFHNNQFGGAIGGPIRRDKTFFFVDYEAMREKGAES
                     STACVPTAQDIATATAANGGPVNPVIAALLAKNPWPAPNLGGSCLTDGTDGQTPVTAN
                     VSLATPFSNRVDSAIVKIDHNINEKNLLTGRYYIGDSSQSFPLALVGGGLVPGYNTAT
                     PTRVQLISISYVSSITPSVVSEVRLGWNRFAEGFFPQDRSFDPTSIGLDTLDPSSGNA
                     SPYNFGLPKISISGLAPIGADNGDPRQRVDTNWHYIDNISWKHGKHDIRFGYEFRRTS
                     ISQIFNRGFRGNLAFSTTTDSTTGLVTATGLQEFLAGTPTGGSQRQGNTNRNTVENSH
                     AGYIQDSYRWKKNVTLNFGLRYDYFGIMQEKQGNFTNVDTATGLGVLVGQSRLYQPDY
                     NNWGPRVSLAWDVTGKGKTVIRAGYGIFYDAFSQDMFMGHLPFNSGFDPGPAYSGFGP
                     NPISSASANPGPITAGFPVYGFASPMGDAFGVDPHIRTPYMQNFNLNFQQELTRKTVF
                     QIGYVGSNGHKLFRFRDINQPTQAQIDAADLACTCINDFSVPRRLSGAPGFSPFFYIN
                     YQESSANSNYNAMQTSLRLTDWHGFTSTVNYTWSHSIDDASDGEDYVPNASQPNDSTA
                     PIGSNRGNSNFDVRNRFTWNFVYQFPNRKGSWQRVTDGWGLNGIVTVQSGQPFQLNYN
                     FEDDYDGSGEGFGRPDVVGPIHYSSDPTQYLDLTSFAVPCTYPASGGSGFADSCAPGS
                     RHFGNEGRNSLIGPHFRQFDFSIFKNTAITERIKLELRFEAYNLFNHPNFANPYLPSF
                     IADAAQNGVGGNGRSLGALRLTATGDVGIGYPFLGSGGPRGLQIAAKISF"
     gene            2737..3321
                     /locus_tag="VII37_10025"
     CDS             2737..3321
                     /locus_tag="VII37_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5070120.1"
                     /translation="MSSPGIAEPTEAIARAKAGDAQAWGQLYHDYAPAIFRFCRRALP
                     TREDAEDATMEVFMKLKGKLNQYDSSRPFSGWLYKVAANHCWDTLRRRKIRQDKETED
                     SDDLPLEHPDPSQLDRLIEQRSSAEVRKALACLSKRARMALVMRYYSDMSYDEIADEL
                     GVRRAFVGVVLLRARHELRNALEGNAALAAGGNA"
     gene            3318..>3634
                     /locus_tag="VII37_10030"
     CDS             3318..>3634
                     /locus_tag="VII37_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5070121.1"
                     /translation="MNDSSNVPDDRLDEHLDNHLDEMSGMLYVEGQLDRNRAQQVSAH
                     AQDCPSCRTLLRALERESRLLTRAMLEEDEPLPPRLAAFQQTAQKSMQWIWGAVFGLA
                     ATGV"
BASE COUNT          761 a         1172 c          918 g          783 t
ORIGIN      
        1 aaccaggtgg gttcaccgca aaatcctttc cacaacaatc aattcggggg agccatcggc
       61 ggccccattc gccgcgacaa aacttttttc ttcgtggact acgaagccat gcgcgaaaaa
      121 ggcgctgaat ccagcaccgc ctgcgtgccc accgcccagg atatcgccac cgctaccgcc
      181 gccaacggcg gtcccgtcaa tccagttatc gctgctcttc tagcaaaaaa tccctggccc
      241 gcccccaacc tcggcggctc ttgcttgacc gatggaaccg acgggcagac tccggtgacc
      301 gccaacgttt ctctggccac gcctttctcc aaccgcgtgg acagcgccat cgtaaagatc
      361 gaccacaata tcaacgaaaa gaatcttctc accggccgct attacatcgg cgacagctcg
      421 caaagttttc cgctggcgct ggtcggcggt ggactggtgc ccggctataa cactgccact
      481 cccacgcgcg tgcaactgat ttcgatttcc tacgtgtcct ccattacgcc gtcggtcgtc
      541 agcgaggtcc gtctaggctg gaaccgcttc gccgaaggat tttttcctca ggatcgcagc
      601 ttcgatccca ccagcatcgg cttggacacg ctcgatccga gctccggcaa cgcctcgccc
      661 tacaatttcg ggcttcccaa aataagcatc tctggacttg cccccatcgg tgccgacaac
      721 ggcgacccgc gccaacgcgt cgataccaac tggcactaca tcgacaatat ttcctggaag
      781 cacggcaagc acgacatcag gttcggctac gaatttcgcc gcacttccat ctctcaaatt
      841 ttcaatcgcg gattccgtgg aaaccttgct ttcagtacga cgaccgactc gacgaccggc
      901 ctggttaccg ccaccggcct ccaggaattt ctcgccggca cgcccaccgg cggttcgcag
      961 cgccaaggca acaccaatcg caacaccgtt gaaaactctc acgccggcta tattcaggat
     1021 agctaccgct ggaagaaaaa cgtcaccctc aacttcggcc tgcgttacga ctacttcggc
     1081 atcatgcagg agaagcaggg aaactttacc aacgtcgaca ccgctaccgg cttgggcgtg
     1141 ctcgtcggcc agtcgcgtct ttaccaaccc gattacaaca actggggacc tcgcgtgagc
     1201 ctggcctggg atgtgaccgg caagggcaaa accgtcatcc gcgcaggata cggaattttt
     1261 tatgacgctt tctcgcagga catgtttatg gggcacctgc cgttcaatag cggcttcgat
     1321 cccggcccgg cctattccgg atttggcccc aaccccatct cctccgccag tgccaacccc
     1381 ggcccaatca cggccggttt tcctgtttac gggttcgcga gccccatggg cgacgccttc
     1441 ggtgtcgacc cgcacatccg cacgccctac atgcagaact ttaatctgaa ttttcagcag
     1501 gagctaaccc gcaaaaccgt tttccagatc ggctatgtgg gctccaatgg ccacaagctg
     1561 ttccgctttc gcgatattaa ccagcctacc caggcgcaaa ttgacgccgc tgatctcgct
     1621 tgcacttgca taaacgactt cagcgttcca cgccgtcttt cgggcgctcc cggattttca
     1681 ccctttttct atatcaacta ccaagagtct tctgccaact ccaattacaa cgccatgcaa
     1741 accagcttgc ggctcaccga ttggcacggc tttacttcca cggtgaatta cacctggtcc
     1801 cattccatcg acgacgccag cgacggcgaa gactatgttc ccaatgcctc gcaaccgaac
     1861 gacagcaccg cgcctatcgg ttccaatcgc ggcaattcca acttcgacgt tcgcaatcgc
     1921 ttcacctgga atttcgttta tcagttcccc aatcgcaaag gcagttggca gcgtgtcacc
     1981 gacggctggg gcctgaacgg catcgtcacc gtgcagtccg gccagccttt tcagctgaat
     2041 tacaattttg aagacgatta cgacggaagc ggcgaaggct ttggccgccc cgacgtcgtt
     2101 gggccaattc actactccag cgatcccaca cagtatctgg acctcacttc attcgcggtc
     2161 ccgtgcacct atccagcctc tggtggttcc ggtttcgcgg atagctgcgc tcccggatcc
     2221 cgccatttcg gcaacgaagg gcgcaactcg ctcatcggcc ctcatttccg ccagttcgat
     2281 ttttctatct ttaaaaatac cgctatcacc gagcgcatca aactcgaact gcgcttcgaa
     2341 gcctataacc ttttcaacca cccgaatttc gccaatccct atctcccgtc ctttattgcg
     2401 gacgccgcgc agaatggtgt cggcggcaat ggccgctccc taggagcttt gcgccttaca
     2461 gctaccggcg acgtgggcat cggttatccg ttcttgggaa gcggcggccc gcggggcctc
     2521 caaatcgccg ccaagatcag tttctaacgc ggtaacagcc tactaaagtc atcaaagagt
     2581 ctggcgtttc gccggactct tctcttttct gatagattgc tgcgtgttta ctgtctccgt
     2641 catttgaacg ggctgtattg gcgcgctcta gccttgccgt gtaagttttt cgccccggct
     2701 gcgtactacc ttatggaagc tctgagacca ggacggatga gttcgccggg aatagcagaa
     2761 ccgacggagg ccatcgcccg agccaaggcg ggggacgccc aggcttgggg ccagctttat
     2821 cacgactatg ctccggcgat tttccgtttt tgccgccgcg cgttgccgac ccgagaagac
     2881 gcggaagacg ccaccatgga agtttttatg aagctgaaag ggaagcttaa ccaatacgac
     2941 tcatcgcggc ccttctcagg ctggctttat aaggtagcgg cgaatcattg ctgggataca
     3001 ctgcgccgcc gcaagattcg ccaggacaag gaaaccgaag atagcgatga ccttcctctc
     3061 gagcatcccg atcccagcca gcttgatcgt ttgatcgaac agcgcagcag cgccgaagtg
     3121 cgcaaggcgt tggcttgcct gagcaagcgc gcgcgcatgg cgctggtgat gcgctactac
     3181 tcggatatga gctatgacga aattgccgat gagctgggcg tgcggcgcgc ttttgtaggc
     3241 gtcgtcttgc tgcgcgcgcg tcacgaattg cgcaacgcac tggaaggcaa cgcggctctc
     3301 gcggcgggag ggaatgcatg aacgattcgt cgaatgttcc agacgatcgc ctggacgagc
     3361 atctcgataa tcacctggat gaaatgtccg gcatgctcta tgtggaaggt caactggacc
     3421 gcaaccgcgc ccagcaagtc tcagcgcatg cccaggattg cccatcttgc cgcacattgt
     3481 tgcgcgccct agaacgcgaa tccaggcttc tgactcgcgc catgctcgaa gaagacgaac
     3541 cgcttccccc gcgattagcc gcctttcagc agaccgcgca aaaatccatg caatggattt
     3601 ggggtgcggt ttttggcctt gctgcgaccg gcgt
//
LOCUS       DATCBB010000915         1212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_126123, whole genome shotgun sequence.
ACCESSION   DATCBB010000915 DATCBB010000000
VERSION     DATCBB010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126123"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VII37_10035"
     CDS             complement(<1..613)
                     /locus_tag="VII37_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5070122.1"
                     /translation="MDEARRIFAAVGIEVELAETTGPGDATEIAQRAAIERRDLVVAC
                     GGDGTLNEVVNGLAGASNGHRVSLALLPAGTANIFAKELSLPWDIPHAARKLAHGKVV
                     DIALGLATPLLEPEKQKYFLSVAGAGPDGRIVYSIDLNLKARLGILAYWWQGAREIFR
                     YDYPHFRVVIDGQSADSSLVIVGRTKHYGGPFKITTLADLYENK"
     gene            809..>1212
                     /locus_tag="VII37_10040"
     CDS             809..>1212
                     /locus_tag="VII37_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070123.1"
                     /translation="MARAGAAIKNEIEDLREKLRHHEYRYYVADDPEISDAAYDRLMG
                     KLKELEVAHPELITADSPTVRVGGSPREGFQTYQHARPMLSLDNAFSYDALREFDRRV
                     RESSGREKIEYIAEHKFDGLSISLQYLGGALVR"
BASE COUNT          278 a          356 c          319 g          259 t
ORIGIN      
        1 atttattttc atacaaatcg gccagcgtcg taattttaaa tggcccaccg taatgttttg
       61 tgcgtcccac gatcactagc gacgagtccg cggactggcc gtcaatcacc acgcgaaaat
      121 gcgggtagtc gtagcgaaaa atttcacgcg cgccctgcca ccagtaagcc agaatgccca
      181 agcgcgcctt caagtttagg tcgatggagt aaacgatcct gccgtccggc ccagcccccg
      241 caacactcaa aaaatatttt tgcttttctg gctcaagtag cggcgtagcc aggcccagcg
      301 caatatcgac cacttttccg tgagctagtt tgcgtgctgc gtgcggtatg tcccaaggaa
      361 ggctcaactc tttagcgaaa atattagcgg tgcccgcggg cagaagcgca agcgaaacgc
      421 gatggccgtt cgacgcgccg gccaggccgt tgacgacttc atttaatgtt ccgtcgccac
      481 cgcacgcgac caccaaatcg cggcgctcga tcgcggcgcg ctgcgcgatc tctgtggcgt
      541 cgcccgggcc tgtcgtttcg gccagttcga cttcaattcc gacggcggca aaaatcctcc
      601 gcgcttcgtc caacgagcgg cgccgtttcg cgcctccgtg tccggcatta ggattgtgaa
      661 tcagaagagc attctgaagc gcaacagcat tctgaagccc ggtagtcatt cgattgaaga
      721 ggtccgtctc agtcttccgt ctattcattc tatataatgt gagagcaatc acagcatctc
      781 ttctacagag atttctgcgc gaggaatcat ggccagggcc ggcgctgcca taaaaaacga
      841 aatcgaagat ctgcgcgaaa agctgcggca tcacgaatat cgctattacg tcgccgacga
      901 cccggaaatt tccgacgctg cctacgaccg gttgatgggc aagctcaagg aactggaagt
      961 cgcgcacccc gaactaatca ccgccgattc gcccaccgtg cgcgtgggcg gctcaccgcg
     1021 agaaggtttt caaacttacc agcatgcccg gcccatgctc agtctggata acgcgttttc
     1081 ttatgacgcg ctgcgagaat tcgaccgccg cgtgcgcgaa agtagcgggc gcgaaaaaat
     1141 cgaatacatt gccgaacaca aattcgatgg cctgagtatt tcattgcaat atctgggcgg
     1201 agcgctcgtt cg
//
LOCUS       DATCBB010000916         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_159455, whole genome shotgun sequence.
ACCESSION   DATCBB010000916 DATCBB010000000
VERSION     DATCBB010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159455"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1184)
                     /locus_tag="VII37_10045"
     CDS             complement(255..1184)
                     /locus_tag="VII37_10045"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY5070124.1"
                     /translation="MTQTENWPNIAVVGAGAVGGYFGGLLARAGAPVTMIGRPAFVDA
                     VNKNGLFLDTLQFQENIRVQASTEISAAAGADIILFCVKTTDNAATARQLAPLLTPGA
                     LVVSLQNGVDNVEQIRAAANLEVLPAVVYVAASVPEPGRIKHVGRGDLTLGPKTEKTE
                     RLATIFTRAGIPCRISDNIAGELWTKLLWNCALNAISALGRVKYGQIAASDDARQVVK
                     SLVEEVFAVATAAGIRLVGVEDASAALAGAIKVATQMSGALSSTAQDMLRGKHTEIDS
                     LNGYISRRGAELVVPTPVNHALYTMIRLLEGRA"
     gene            1285..1843
                     /gene="rnpB"
                     /locus_tag="VII37_10050"
     ncRNA           1285..1843
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VII37_10050"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          462 a          588 c          568 g          406 t
ORIGIN      
        1 gtgatctccg cattccggaa ttgctctctc ttgtgtgaag aatcagctgg gggcgcctgg
       61 caccacatcg gtgccgaaca tgcagtcgcg gcgacactaa cagctaaccc taataatgtc
      121 ctgaagcgaa ttcgagacac gtgtctcctt ccgatcgcag caccaacgcc cgtgatggac
      181 gaatgcaacc cgcaaaggtg agcgtgtttc gggagagatg gctgtaatag ccctgattcg
      241 cttctaaccg tgccttacgc gcggccttcg agcaacctaa tcatcgtgta gagcgcatga
      301 tttaccggcg tcggtacaac tagttccgcg ccgcgccggg aaatataccc gttcagcgaa
      361 tcgatctcag tgtgcttgcc acgcagcatg tcctgcgcgg tcgacgacaa agctccggac
      421 atttgtgtcg ccaccttgat tgcacctgcg agtgcagcgc ttgcatcttc cactcccaca
      481 aggcgaatgc cggctgcagt cgccaccgcg aaaacttctt ctaccagcga tttgacaact
      541 tgccgcgcgt catcgctggc ggcaatttgc ccgtacttca cccgtcccag ggcggaaata
      601 gcgttcagcg cgcaattcca aagcagcttg gtccatagtt cgcccgcaat attgtcggag
      661 atacgacaag ggatgcccgc gcgcgtaaaa atagttgcga gacgttcggt tttttcagtt
      721 ttcggtccaa gcgtgagatc tccgcgaccc acatgcttga ttctcccggg ctcgggtacg
      781 gaggccgcga cataaacgac cgcaggcaag acttccagat tggctgcagc acgaatctgt
      841 tccacgttat cgacgccatt ttgcaggctg acaaccaaag caccgggcgt gagcaacggc
      901 gccagttgcc tggcggtagc ggcgttgtcc gtagtcttca cgcagaacaa aataatatcc
      961 gcgccggccg ccgcggatat ttccgtggac gcctgcacgc gaatgttttc ctgaaattgc
     1021 agagtatcca gaaaaaggcc gtttttattt acggcgtcaa cgaaagctgg acgcccaatc
     1081 atggtcacag gcgctccagc ccgcgccagc aggccgccaa aatatcctcc caccgcgcct
     1141 gctcccacga ccgcgatatt cggccagttt tctgtctgtg tcatgtcatg gtcaccgcaa
     1201 attcagccgt cgagttcaac agcatagccg ccgcatattg tacatgcgtc aaagcggtgg
     1261 acttgcaggg tatacttaaa acagcgggcg ggccgggtaa tcgctggctg ctgggccgca
     1321 aggcgcagca gcgggaggaa agtccggaca ccgtcctgag gcatggccaa cgattcgttg
     1381 gccagaactc ttgacagggc aacgcgcctg gtaacgccag ggggcttcgc tataaggcga
     1441 agctacggat agtgccacag aaaattaaac cgccggcgga gcgcagcacg gtgacatcgc
     1501 gcaggcaaaa cctgcgagac gtctcgcaat gctcgcactg ttggtaaggg tgaaaaggtg
     1561 gggtaagagc ccaccgcttc gctagcgata gcgagggcac ggaaaacccc gcgtggtgca
     1621 aggccaaata ggaggggaga gctggcccgg ctcattgagc ggcgttcctg caaaggaacg
     1681 cactccattt tatgcggcgc aagccgcgta aattgaaaac cctcgggtag gctgctggag
     1741 tcccggtgcg aaccgggacc tagaggaatg attgccgcgg tttgcagcgc gccatgcgcg
     1801 atgcaacgcc gcacagaatc cggcttacag gcccgtccgc tattttttca ataactcaca
     1861 gacagttttt agcgaagccg tgaaaaactg tgacacacat ctccgctaac cattcaccaa
     1921 acccctactc gaataactgc gaagcaagaa ggagagcctg catgccatcg aatcatctgc
     1981 ggttgggagc tggattcgcg acgttgttgt gcctgggatt gctg
//
LOCUS       DATCBB010000917         1133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_126217, whole genome shotgun sequence.
ACCESSION   DATCBB010000917 DATCBB010000000
VERSION     DATCBB010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126217"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VII37_10055"
     CDS             complement(<1..313)
                     /locus_tag="VII37_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070125.1"
                     /translation="MFAALLLFTFHLVVPAAISAGQAAERQADRTAIRAVLDAQQEAW
                     NRGDIDAFLTGYWHSPDLTFSGTNGISRGWDAVLARYKKNYPDRSAMGTLDFSNLEFR
                     FL"
     gene            530..934
                     /locus_tag="VII37_10060"
     CDS             530..934
                     /locus_tag="VII37_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070126.1"
                     /translation="MFAALLISISTVAIAQFGMYYWRAVLASVASLPVSESVLAAISA
                     ENRPLEAADFVQFAGLHELTPDLAPRGSGLGLVRFYYGLTRAANMLIGVRVPAIAQWI
                     EQEGAICARYAAVQIDQRLQANMELAAALRSC"
BASE COUNT          265 a          273 c          344 g          251 t
ORIGIN      
        1 ccaggaagcg aaattccaag ttggaaaaat cgagcgtgcc catggcgctg cgatcagggt
       61 aatttttttt gtaacgcgcg agaacagcat cccagccacg ggagatgccg ttggttcccg
      121 aaaaagtaag atccggtgaa tgccagtagc cggtgagaaa tgcatcgatg tcgccgcgat
      181 tccaggcttc ttgttgggca tcgagtacgg cacgaatggc agtgcggtcg gcctggcgct
      241 cggctgcttg gccggcggat atggcggctg gcaccacgag gtggaaagtg aagagcaaaa
      301 gtgccgcgaa cattgttgcg ttctggaggt aacgggaatt gggcatgtac tcacctggta
      361 gacgcgcggg gcaggataat cgaaagccag ggaatcggca atgcataggg gtgacatgcg
      421 tgcaagacca atgaattcag gtagttaagc gatagttcca tgggcttggt actgctgagc
      481 tatagggcaa atcagagggg gacgcgagaa tacgggtgag gaaaaaagca tgttcgccgc
      541 tttgctcatc tctatatcga ctgtcgcgat agcccaattt gggatgtatt actggcgggc
      601 tgttctagcc agcgtcgcct ctctaccggt ttctgagtca gtgctcgcag ccatctccgc
      661 ggagaaccgg ccgttagaag cggctgactt cgtgcagttt gccgggctgc atgaattgac
      721 accagattta gctccacgtg gcagcggcct gggattggtg cgcttttatt acgggctgac
      781 gcgcgctgca aatatgctga tcggcgtgcg ggttccggcg atcgcgcaat ggatagaaca
      841 agaaggcgcg atttgcgcgc ggtatgccgc ggtgcagatc gatcaacggc tgcaagctaa
      901 catggagttg gctgcggcgc tgcgatcctg ctaacggggc gcttcctcgc aatgcaaaat
      961 cccagccgca aatatggcgc ctgggaatac tcaatctgag acattcttct caactgttta
     1021 agagtttggg tttaccttct gaccagctta tatttgcttc agcgccattt gcttccgacg
     1081 aagacctggc actcattaga aacacgccaa acagaaattg cagccaaata aat
//
LOCUS       DATCBB010000918         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_385062, whole genome shotgun sequence.
ACCESSION   DATCBB010000918 DATCBB010000000
VERSION     DATCBB010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385062"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..555
                     /gene="gcvH"
                     /locus_tag="VII37_10065"
     CDS             172..555
                     /gene="gcvH"
                     /locus_tag="VII37_10065"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY5070127.1"
                     /translation="MAYPKDYKYTREHEWIQVKGNTATVGITDHAQESLGDIVFVELP
                     KVGAQIEAGKSFGTVESVKAVSELYAPASGKVVEINESLDQTPEAVNKDAHSAWMIKL
                     ELTNPSQADGLMSADDYEKYVAEEK"
     gene            709..2034
                     /gene="gcvPA"
                     /locus_tag="VII37_10070"
     CDS             709..2034
                     /gene="gcvPA"
                     /locus_tag="VII37_10070"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY5070128.1"
                     /translation="MRYLPKSPADRAHMMREIGCKSIDDLFAPIPAEYRLSRDLKVPR
                     QMAESEIIDYFHARADNAAAGYALFLGAGAYHHYRPVVIDAMVSRGEFLTSYTPYQAE
                     IAQGTLQAIFEFQTMICELTGMEVANASMYDGSTGAAEAMLMAVRITGRKAAVVARSV
                     HPEYREVMHTYTQHQGLPITECDYLENGRIDMADLEKKITSDTACVLIQSPNFFGTIE
                     DVAAVADLVHKKGALLVVSIAEALSLGIVKPPVEADIVSMETQSFGVPLGYGGPYAGV
                     MATKEKFVRQIPGRLVGETKDKDGKRGFVLTLSTREQHIRREKATSNICTNQALIATM
                     ATIYLTIYGREGLKELAQQNLAKATYASSEFAKKGKILFDGAPRFNEFVVKTKDDAHA
                     INKRLLEKKMIGGFPLSWHYPELGNASLWCCTELNSKEQIDAAVQAVGQ"
BASE COUNT          568 a          524 c          641 g          397 t
ORIGIN      
        1 tcgcattcgg agatcgggca agaaattttt gtcgagatcc gcggccagaa gatcaagagc
       61 aaggttgtgc caacgccgtt ttacaagcgg cctaaaaaat cataggtcca gaaacggacc
      121 ccagaaatct tagctccaga aaaatcttaa ttgcaggaca ggatgaaccg aatggcatat
      181 ccgaaagact acaagtacac ccgagagcac gagtggatcc aggtgaaagg caacacggcg
      241 acggttggaa ttacggacca cgcgcaggaa tcgctgggcg acattgtgtt tgttgaactg
      301 cccaaagtag gcgcgcagat tgaagcgggc aagagctttg gcacggtgga atcggtaaaa
      361 gccgtttcag aactttacgc accggcatcg ggtaaggtgg ttgagatcaa tgaaagcctg
      421 gaccagacgc cggaagccgt gaataaagac gcgcacagcg cctggatgat caagctggaa
      481 ttgacgaacc cgagccaagc ggacgggttg atgtcagctg acgattacga gaagtacgtg
      541 gcggaagaga aataatattc accacggaga tggtttccga agtcccgagc gaagcgaggg
      601 atcactacac tcacgaagaa tttcaaggtt tttgaaggaa taagttgagg tctcgacagc
      661 aacgaaggga atagtgatcc ctcgcttcgc tcgggacttc ggagaaacat gcgctactta
      721 ccaaaatctc ccgctgatcg cgcgcacatg atgcgcgaga tcggatgcaa gtccattgat
      781 gacctgttcg cgccgattcc ggcggaatac cggctgagcc gcgacctgaa agtgccgcga
      841 cagatggcgg aatcagagat catcgactat ttccatgccc gcgcggataa cgccgcagca
      901 ggctacgcgc tgtttctggg cgcgggcgcc taccatcatt accgcccggt ggtaattgac
      961 gcaatggtgt cgcgcggcga gtttttgacg tcctatacgc cataccaggc ggagattgcg
     1021 cagggaacac tccaggccat cttcgaattc cagacgatga tctgcgagct taccggaatg
     1081 gaagtggcga acgcttctat gtatgacggt tcaaccggcg cggcggaggc catgctgatg
     1141 gccgtccgca ttaccggcag aaaagcagcg gtggtggcgc gcagtgtgca tccggaatac
     1201 cgcgaggtaa tgcataccta cacgcagcac cagggcctgc cgattacaga gtgcgactac
     1261 ctggaaaacg gtcgcattga catggccgac ctggaaaaga aaatcacgtc ggacacggcg
     1321 tgcgtgctga tccagtcgcc aaacttcttt ggcacgattg aagacgtagc ggccgtggct
     1381 gacctggtac acaaaaaggg cgcgctgctc gtggtctcga ttgcggaagc gctgtcactg
     1441 ggaattgtga agccgccggt ggaagcggat attgtgtcga tggagacgca gtctttcggc
     1501 gtacctctgg gatatggcgg accatacgcc ggagtgatgg cgacaaaaga aaaatttgtc
     1561 cggcagatcc cggggcgact tgtaggtgag acaaaagata aagacggcaa acgcggattt
     1621 gtgctgacac tttccacgcg cgagcagcat atccgccgag agaaagccac ttcgaacatc
     1681 tgcaccaacc aggcgttgat tgccaccatg gccacgatct acctgacgat ctatggtcgg
     1741 gaagggctga aggagctggc ccagcagaac ctggccaaag cgacgtatgc gtcgagcgag
     1801 ttcgcgaaaa agggcaagat cctgtttgac ggcgcgccgc gcttcaatga gtttgtggtt
     1861 aaaacgaaag atgatgcgca cgcaattaac aaacgcctgc tggaaaagaa aatgattggc
     1921 ggcttcccgt tgagctggca ttatccggaa ctgggcaacg catcgctgtg gtgttgcacg
     1981 gagttgaaca gcaaagaaca aattgacgca gcggtgcagg cggtgggcca atgagcaata
     2041 aagacatcaa aaaagcaacg cgccacgtta accagaatga aggactgatc tttgagcgtt
     2101 cgtcgccggg gaagaaaggt taccggctgg
//
LOCUS       DATCBB010000919         1244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_159493, whole genome shotgun sequence.
ACCESSION   DATCBB010000919 DATCBB010000000
VERSION     DATCBB010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159493"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VII37_10075"
     CDS             <1..919
                     /locus_tag="VII37_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEY5070129.1"
                     /translation="SGHAFVSHKAHVLYNPYVSIFRDFGATLEMIKWEHSIFALPFAL
                     CGALLAARGVPTAHQLIWIIAAMITARSAAMAFNRLADATTDAANPRTKMRALPGGVL
                     TRNFVALFVAASCVGFVFAASRLNRLTFALSPIALAVVLLYSYTKRFTRWSHLVLGFA
                     LGIAPAGAWIAVRGSLDPRILLLTAAVMFWVAGFDVLYACQDYDFDSQAGLHSIPRYC
                     GIARSLWIARLFHAAMLALLIGLVIAFNLGVLALIGVAAVAALLFYEHTLVHANDLSK
                     LNAAFFTMNGVISIIFFAFIAGDVLLRKA"
BASE COUNT          234 a          317 c          349 g          344 t
ORIGIN      
        1 gtccggccat gcgttcgtta gccacaaggc tcatgtgctt tacaatccct atgtgagtat
       61 tttccgcgat tttggcgcta cgctggagat gattaagtgg gagcactcta tcttcgcgct
      121 cccatttgcg ctgtgcggag cgctgctcgc ggcacgcggg gttccaactg cccatcagtt
      181 gatttggatt atcgctgcga tgatcacggc gcgttcggcg gcgatggctt ttaatcgact
      241 agccgatgcc actaccgacg cggccaaccc aagaacaaag atgcgcgctc ttccgggtgg
      301 cgtgctcact cgaaattttg tggctttatt tgtggccgca tcttgtgtgg gatttgtgtt
      361 tgccgcatcg cggctcaatc gtctgacgtt cgcgctgtcc ccaatcgcgc ttgcagttgt
      421 gctgctttat tcatacacca agcgatttac tcggtggtcc cacttagtgc tcggtttcgc
      481 cctcggcatt gctcccgccg gggcatggat cgcggttaga ggatcattgg accccagaat
      541 tctattgttg acggcggcag tgatgttctg ggtggcgggg tttgacgtgt tgtatgcgtg
      601 ccaggattat gattttgact ctcaggcagg attacattcg attccgcgct actgcggaat
      661 tgcgcggtcc ttatggattg cgaggttgtt tcatgcagcc atgctcgctc tgctaattgg
      721 cctcgttatc gcgttcaact tgggtgtctt ggcgctgatc ggggtggccg cagttgccgc
      781 gcttctgttt tatgagcaca cgctggtcca tgccaacgat ttgagcaaac tcaatgccgc
      841 atttttcacc atgaacggcg tcatctcgat catattcttc gcttttatcg cgggagatgt
      901 gttgctgcga aaagcataga gcgtgtttca taaatcggtt ttgggtaggg cacggctttt
      961 agccgtgccg cttagacgct ggagaaatgc gggctttagc ccctgaggca gcgggccttc
     1021 caagcggcaa cgtatttatg aaacacgctc caagaaatgg atggaataag gcatgcttcc
     1081 ggtgactgga agcggcttgc tacagtatca tcatagaaat ggcgcattca tttcagacgg
     1141 acgattcccg gcttgaggcc atacacgaaa aggtaatcgc tcgcgaccgt ttaaccgaag
     1201 aggacgcact aactctttat cgcactgccg acattcttgc tgtg
//
LOCUS       DATCBB010000920         1013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_224438, whole genome shotgun sequence.
ACCESSION   DATCBB010000920 DATCBB010000000
VERSION     DATCBB010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224438"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1013
                     /gene="metK"
                     /locus_tag="VII37_10080"
     CDS             <1..>1013
                     /gene="metK"
                     /locus_tag="VII37_10080"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY5070130.1"
                     /translation="ETLTATGLVVIAGEITTKAYVDFQSLVRGMISSIGYNNALYGFD
                     SNTCAVISSINKQSGDIAMGVDTGGAGDQGMMFGYATDENAELMPTPILLAHKLAMRL
                     SEVRKSGKMNYLRPDGKSQVTVEYDKNHKPVRVDAVVVSTQHSETVGNDELRADILKN
                     VIQATIPTHLLDADTKYHINPTGRFVIGGPMGDTGLTGRKIIVDTYGGMGRHGGGAFS
                     GKDPTKVDRSAAYMARHIAKNIVAAGLAERCEVQLAYAIGVADPVSVLVETFGTCKVD
                     PALLPDLVRANFKLTPKGIIDSLRLRRPIYCKTAAYGHFGRNDPDFTWEAVDKASQLA
                     SAA"
BASE COUNT          223 a          239 c          320 g          231 t
ORIGIN      
        1 cgaaacccta accgcgacag gcctggtagt aatcgccggc gaaatcacca caaaagctta
       61 cgtggatttc caatcgttag ttcgcggcat gatctcctcc atcggctaca acaacgccct
      121 atacggcttc gactccaaca cctgcgccgt tatctccagc atcaacaaac aatccggcga
      181 catagccatg ggcgtagaca caggcggcgc cggcgaccag ggaatgatgt ttgggtatgc
      241 gacggatgag aatgcggagt tgatgcctac gccgattctt ttggcgcata agctggctat
      301 gcggctttcg gaagtgagga agagcgggaa gatgaattac ttgcggccgg atgggaagtc
      361 gcaggttact gtggagtatg acaagaatca taagccggtg cgggtggatg ccgtggttgt
      421 ctcgacgcag cactcggaga ccgtgggtaa tgacgagctt cgcgctgata ttttgaagaa
      481 tgtgattcag gctactattc ctactcatct tttggatgcg gacaccaagt atcacattaa
      541 tcctactggg cgatttgtga ttggcgggcc tatgggagac accgggctta ctgggcggaa
      601 gattatcgtt gacacttacg gtgggatggg caggcacggc ggcggcgcat tcagcggaaa
      661 agatccaacc aaggttgatc ggtcggcagc ttacatggcg cggcacatcg caaaaaatat
      721 cgtcgcggcg gggttggctg agcgctgcga ggttcagttg gcttacgcga ttggagtggc
      781 tgatccggtg agcgtgctgg tggagacgtt tgggacttgc aaggttgatc cggcgctgct
      841 tcctgatctg gtgcgggcga atttcaaact tactccgaag gggattatcg attcgcttag
      901 gctgcggcgt ccgatttatt gtaagactgc ggcttatggg cattttgggc ggaacgatcc
      961 ggatttcacc tgggaagcgg tggacaaagc ttcccagctt gcgagtgccg cgg
//
LOCUS       DATCBB010000921         1513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_190396, whole genome shotgun sequence.
ACCESSION   DATCBB010000921 DATCBB010000000
VERSION     DATCBB010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190396"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..854)
                     /locus_tag="VII37_10085"
     CDS             complement(153..854)
                     /locus_tag="VII37_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5070131.1"
                     /translation="MTELQSASTKPATPRELIHITLPVGMLHCNCTIIGDPLTREALV
                     IDPGDEVTRIMDLLGRHKLTVKAIISTHAHIDHVGGLSKLHQFTGAPVLMHQEDLPLY
                     QAMDVQAAFLGVPAPEITSIDNLLKEGDTLRWGDYQARIIHTPGHTPGSVCLYVPQEA
                     GTVTLAAPQLLAGDTLFAGSIGRTDLWGGSMPQIMDSLRSKLMQLPDSTIVYPGHGPA
                     TTIGTERHSNPFLIA"
     gene            980..>1513
                     /locus_tag="VII37_10090"
     CDS             980..>1513
                     /locus_tag="VII37_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070132.1"
                     /translation="MQNAEATIETPLAGVHRRAGATMGVWFGCALPDDFGDAREEQRF
                     ANESVALIDKNYRAYFTFSGPDRVRYLNAILTNHIKDLTSGQGVVSLLLNAQGRILAE
                     IETFAEAEKIFLVSYLMNRERLAEVLEKYIIMDDVTFSDDAEKFGTLAVEGPRAAEVV
                     EELTGVDLNSLLQLGWAD"
BASE COUNT          303 a          309 c          532 g          369 t
ORIGIN      
        1 tcgcaaagcc ggacggggtg agaatcgaag aagtaaagta aggctgttct tagatcaccg
       61 attctgtctg acatgcgtga ttaacgccgc aacccaaaac tagcccgcta ttaaaaatcg
      121 cgatgggaaa tagtccttca ttggaaaaat cgtcaagcga tgaggaaggg attggagtgg
      181 cgttcggtgc ctatggtggt ggcggggccg tggcctgggt agacgatggt gctgtcgggt
      241 aattgcataa gcttacttcg gagtgagtcc atgatttgcg gcatggagcc gccccataaa
      301 tctgtgcggc ctatgctgcc tgcgaagaga gtgtcgcctg ctaagagttg cggggcggcg
      361 agagttacgg tgcctgcttc ctgcgggacg tataggcaga cgcttcctgg agtgtggccc
      421 ggggtgtgaa tgatgcgggc ctggtagtcg ccccagcgta gggtgtcgcc ttctttgagc
      481 aggttgtcga tgctggttat ttcaggggcg gggacgccta gaaacgcggc ttgcacgtcc
      541 attgcttggt acagcggcaa atcttcttga tgcatgagga ccggggcacc ggtgaattga
      601 tgcaatttgc tgagtccgcc gacgtggtcg atgtgggcat gagtgctgat gatggctttt
      661 acggtgagtt tgtggcgacc gagtaaatcc atgatgcggg ttacttcgtc gccgggatcg
      721 atgaccaggg cttcgcgggt gagcggatcg ccgatgatag tgcaattgca atgcagcatg
      781 cctaccggga gggtgatgtg gatcagctcg cgcggggtgg caggcttggt gctggctgat
      841 tgcagttcgg tcatttgatg gacgctcggc tgatgaatta tagcattcga ggagtttcgg
      901 ttttcgtggc gcggacgctg tggggcagcc gtttgctaaa ctgtatatga ggctcttgga
      961 cttgagcaag gaattggcga tgcagaacgc tgaagcaacg atcgagactc cgctggcggg
     1021 ggtgcatcgc cgcgctggag ctacgatggg ggtgtggttt ggttgcgcgc tgccggatga
     1081 ttttggggat gcgcgggaag agcagcggtt tgcgaatgag agtgtggcgc tgatcgacaa
     1141 gaattaccgg gcgtatttca ctttttccgg gcctgaccgg gtgcgctatt tgaacgcgat
     1201 tctcactaac catattaagg atttgacaag tgggcagggc gttgtttcgc tgttgctgaa
     1261 tgcgcagggg cgcattctgg cggagataga gacgtttgcc gaagcggaga agattttttt
     1321 agtttcgtat ttgatgaacc gggagcggct ggcagaagtg ttggagaagt acatcatcat
     1381 ggatgacgtt acgttcagcg atgatgcgga gaaatttgga acgctggcgg tagaagggcc
     1441 gcgcgcggcg gaagttgtgg aagaactcac gggcgtggat ttaaattctt tgctgcagct
     1501 tggttgggcg gac
//
LOCUS       DATCBB010000922         4001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_353140, whole genome shotgun sequence.
ACCESSION   DATCBB010000922 DATCBB010000000
VERSION     DATCBB010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353140"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /gene="aspS"
                     /locus_tag="VII37_10095"
     CDS             complement(<1..1511)
                     /gene="aspS"
                     /locus_tag="VII37_10095"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY5070133.1"
                     /translation="MSVANNGPSNEAFEEIKVLLDFLGNLKRTNYCGELRAKDSGRDV
                     VVMGWVARRRDLGNLLFLDVRDRSGIVQVVFNKETHPEAHAKAEQARSEFVVAVEGQV
                     IQRQKSNPELATGEIEIVASRLHILNTAKTPPFQIEDDINAAEETRLRYRYLDLRRPR
                     PHRNLALRHKIFLEIRRTMDELGFLEIETPMLTRSTPEGARDYLVPSRVHHGQFYALP
                     QSPQIFKQILMIGGLDRYFQIVKCFRDEDLRADRQPEFTQLDVEMSFPRQEDIFGVIE
                     SVMARACAVAGIKAGAPFPHMLYKDAVRKYGSDKPDLRFGMELHEVTEAFPSEAKSKL
                     QIEGNVFAFAARGAAGYSRKQLDELTEKAKAAGARGAYFVKVASEGATSTVEKLIGAE
                     NVKKLVELCTAKTGDLIVAVSAKEQIKGTEAAALVAGQLRLQLGEALGVIDKTQWKFL
                     WLTGFPLFEWSNSDKTWVSAQHPFTGIVDEDLEKLESAPWEVRSKGYDLVLNGV"
     gene            complement(1486..2817)
                     /gene="hisS"
                     /locus_tag="VII37_10100"
     CDS             complement(1486..2817)
                     /gene="hisS"
                     /locus_tag="VII37_10100"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY5070134.1"
                     /translation="MSESSATGQQSKPAAKPRKFQAIKGVRDLLPAETPLWNHVEETA
                     HAVFPTFGFSEIRLPIFEPAELFARSIGLDTDVVSKEMYTFADRDESMISLRPEATAS
                     VCRAYIEHSMQQLPQPVKLYYMGPMFRRERPQKGRYRQFYQIGAEVLGGSDAPGIDAE
                     VIDMLMTFFDKLQLQGLELDINSIGCRECRAKYVEVLRSELLKVRDKLGADSQRRIET
                     NPLRVLDSKLDSEQEIIATLPRMAAHLCAACAEHFAEVKRQLELRGVLYRENWRLVRG
                     LDYYMRTTFEITAQGLGSQNAVCGGGRYDGLVELLGGPPTKGIGFAIGEDRLILSLQE
                     SGKAPAPLGRDIYIAWMGEKAFATAIRAAKDLRAAGYRVELPAIEQKFGKALGQADRL
                     GSRYALILGEDEVTSGEWTLKTLADGSQEKYTETELLEYLRDTNVGGQQRT"
     gene            complement(2810..3346)
                     /locus_tag="VII37_10105"
     CDS             complement(2810..3346)
                     /locus_tag="VII37_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY5070135.1"
                     /translation="MARVPDIRAFRGKWPQIAASAWIDPAAVIIGDVTIGEDASVWPG
                     TIIRGDVHYIRIGARTNVQDGCVLHVMRDEWPLVLGDDVTVGHSVTLHGCTIESLCLI
                     GMGAIILNGARIGAGSIIAAGTLVTERTVIPPGSLVMGAPARVKRSLTEPDRASIADY
                     AKRYVQYKNVYREEAASE"
     gene            complement(3493..>4001)
                     /locus_tag="VII37_10110"
     CDS             complement(3493..>4001)
                     /locus_tag="VII37_10110"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY5070136.1"
                     /translation="HSVITQFLRSVDWGELDYLLIDLPPGTGDVQLTLIQTVAVTGAV
                     IVTTPSTVALADVRKAIEMFRQVNVEVLGVVENMSTFSCPHCGKAVDIFGHGDGATTA
                     REYGVPLLGEIEIDPRIRVGGDTGKPVAAAGENGAGAKSLFGLARAVTARLEEVAASA
                     TAPSVQID"
BASE COUNT          834 a         1174 c         1074 g          919 t
ORIGIN      
        1 acgccattca aaaccagatc gtagcccttg gagcgaactt cccatggcgc ggattcaagt
       61 ttctccaggt cctcgtccac aatcccggtg aaggggtgct gtgcgctgac ccaggttttg
      121 tcggaattgc tccattcgaa aagagggaag ccggtgagcc acagaaattt ccactgagtc
      181 ttgtcgatca cgccgagcgc ttcgcccagt tgcaaacgca attgtccagc gaccagagca
      241 gcagcttccg tgcctttgat ttgttccttg gccgagacgg ccacgattag gtctccagtt
      301 ttcgcggtgc agagttccac gagtttcttt acgttttcag cgccaatcaa tttctcaaca
      361 gtggaggtcg caccttcaga cgcgaccttt acaaaataag cgccccgcgc gcctgcggct
      421 ttggccttct cggtgagctc atccaattgt ttgcgcgaat atccagccgc gccacgagcc
      481 gcaaacgcaa aaacattgcc ttcaatctgc agcttcgact tggcctcgga aggaaaagct
      541 tcggtgacct cgtggagttc catgccaaaa cgcagatcgg gcttgtcgct gccatatttg
      601 cggacggcgt ccttataaag catgtgcgga aatggtgccc cggctttaat ccctgccacc
      661 gcgcaggcgc gtgccatcac gctttcgatc acaccgaaaa tgtcttcctg gcgcggaaag
      721 gacatctcca cgtccagctg agtaaattcg ggctggcggt cagcacgcaa atcctcgtcg
      781 cgaaaacact tcacgatttg aaaatagcgg tccaagccgc cgatcatcag gatttgctta
      841 aaaatttgcg gcgattgggg caaggcgtaa aactggccgt gatgcacgcg gctgggtacc
      901 agatagtcgc gagcgccttc cggggtagag cgggtcaaca taggcgtttc gatttcaaga
      961 aatcccaatt cgtccatggt gcggcgaatt tccaagaata ttttgtggcg caacgccaag
     1021 ttgcggtgcg gacggggacg acgcagatcg agatagcggt agcgcagccg ggtttcctcg
     1081 gcggcgttga tgtcgtcttc aatttgaaac ggcggcgttt tcgcggtgtt taaaatatgc
     1141 agtcgcgaag ccacgatctc gatttcacca gtcgcgagtt ctggattgga tttctggcgc
     1201 tggatgactt ggccttcgac ggcgaccaca aattccgagc gcgcttgttc cgccttggca
     1261 tgcgcttccg gatgcgtttc cttgttgaag actacctgca cgattccgga gcgatcgcga
     1321 acgtccagaa acagcaagtt gccgagatcg cgccgacgcg cgacccatcc catcacgacc
     1381 acatcgcggc ctgagtcttt tgcgcgtaac tcgccacagt agtttgttcg cttcaaattc
     1441 ccgagaaaat ccagcaagac ttttatctcc tcaaaagctt cattactagg tccgttgttg
     1501 gccaccgaca ttagtgtctc gcagatactc cagcaactcg gtctcggtat atttttcctg
     1561 cgatccgtcg gccaaggtct tcaaggtcca ctccccggag gttacttcgt cttcgcctaa
     1621 tataagcgcg taacgggagc ctagcctatc tgcctgacca agtgccttgc cgaatttctg
     1681 ctctattgct ggcaattcca cacgatatcc tgctgcgcgt aaatctttcg cggcgcgaat
     1741 cgccgtggca aatgcctttt cgcccatcca cgcgatgtag atgtcgcgtc cgagcggtgc
     1801 cggagctttg ccactttcct gcagcgataa aatcaagcga tcttcgccga ttgcgaagcc
     1861 gattcccttc gtgggcgggc ctccgagcaa ctccaccagg ccgtcatagc gaccgccgcc
     1921 gcaaacggcg ttctgcgagc ccaagccttg cgcggtgatc tcaaatgttg tgcgcatgta
     1981 atagtccagc ccgcggacga gcctccagtt ttcgcgataa agtactccgc gcaattccag
     2041 ttgacgcttc acttctgcga agtgctccgc gcaggcggcg caaagatgcg cggccatgcg
     2101 tggcaacgta gcgataatct cctgctcgct gtccagtttg gaatcgagta cgcgcaacgg
     2161 attggtttcg atgcgccgtt ggctgtcggc gccgagtttg tctctcactt tcagaagttc
     2221 gctacgcagc acttccacgt atttcgcgcg acattcgcgg caccctatgg agttgatgtc
     2281 cagctccaag ccctgcagtt gtagcttgtc gaagaaggtc atcagcatgt cgatgacttc
     2341 ggcgtcgatt ccaggagcgt cgctcccgcc caaaacttct gcgccaatct ggtagaactg
     2401 gcgatatcgg cccttttgtg gccgctcacg ccgaaacatc ggacccatgt aatacagctt
     2461 caccggctgt ggaagctgct gcatgctgtg ttcaatgtag gcgcgacaaa cgctggcggt
     2521 ggcttcagga cgtaaactga tcatggactc gtcgcgatct gcgaaggtat acatttcctt
     2581 ggacactaca tcggtatcga ggccgatgga gcgcgcaaac aattccgcgg gttcaaaaat
     2641 tggcaggcga atttcgctga acccaaatgt cgggaaaacg gcgtgtgcgg tttcctctac
     2701 gtggttccaa agcggcgttt ccgccggcag caaatcccgc acgcctttaa tggcttgaaa
     2761 tttgcgaggt ttggccgcgg gcttggattg ctgtccggtt gctgaacttt cactcactcg
     2821 cggcttcctc acgataaaca ttcttgtatt gcacgtaacg cttggcgtaa tcggcaatcg
     2881 aagcgcgatc cggttccgtc agcgaccgtt tcacccttgc tggcgcgccc atcaccaagc
     2941 ttccaggtgg aatcacggtg cgctccgtaa ccagcgtgcc tgcggcgatg atactgcccg
     3001 cgccaattct cgcgccgttc aggatgatgg cgcccatacc gatcaggcag agcgattcga
     3061 tggtgcagcc gtgcagcgtc acggaatgcc ccacggttac atcgtcgccc agcaccagcg
     3121 gccactcatc gcgcatgaca tgcaaaacgc aaccgtcttg cacgttagta cgcgcgccaa
     3181 tgcgaatgta atgtacgtcg ccacgaataa tggtgccggg ccacacgctg gcatcttcgc
     3241 cgatggtcac gtcgccgata attactgcgg cgggatcaat ccatgcgctg gcagcaattt
     3301 gcggccactt cccgcgaaag gctcgaatgt ccggaactct ggccaatatt ccccatggtt
     3361 agcagcctga gacgcgatgg ccaccgttag cttgtcttct atcttaatgg ggattgtgtt
     3421 tcgagcaaag aaatcgagta tgtggaatag cagcggctaa tcgttttgag ccgcgagagc
     3481 gacattccgt aattagtcga tttgtaccga cggggccgtc gcgctggcag cgacttcctc
     3541 taaccgcgcg gtcaccgcgc gcgccaggcc aaaaaggctt tttgcccctg cgccgttttc
     3601 gcccgcggcg gccaccggct tgccggtatc gccgccgaca cgaatgcgcg gatcgatttc
     3661 aatttctccc aggagcggca cgccatattc tcgcgcagtt gtggcgccgt ccccatgacc
     3721 aaaaatgtcc acggctttgc cgcagtgtgg gcaagaaaac gtgctcatgt tttcgactac
     3781 gcccaacact tctacgttca cttgacgaaa catttcaatg gctttgcgca catcggccaa
     3841 cgccacggtt gaaggggtgg taacaattac agcgcccgtt accgcaacgg tttggatcag
     3901 tgttagttgc acgtcgccgg taccgggcgg cagatcgatc agcaaataat ccagttcgcc
     3961 ccaatcgacg ctgcgcaaaa actgcgtgat cacgctgtgc a
//
LOCUS       DATCBB010000923         1251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_418609, whole genome shotgun sequence.
ACCESSION   DATCBB010000923 DATCBB010000000
VERSION     DATCBB010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418609"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VII37_10115"
     CDS             <1..472
                     /locus_tag="VII37_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5070137.1"
                     /translation="TTAPPMPLERTFAHMGLNAYLKKLPHPEPQVPENEASYMPGAQV
                     YKENCAVCHGLPDQPKTNIAEGMAPGPPQLFKGKGVTDDEAWETYWKVESGIRMTGMP
                     GFKGKLTEPQIWQVSVLVKNADKISPAVKAALLSSEPSATPVIPANPTHVPPTR"
     gene            complement(469..>1251)
                     /locus_tag="VII37_10120"
     CDS             complement(469..>1251)
                     /locus_tag="VII37_10120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5070138.1"
                     /translation="PALGGVAYAAFHGPAFVYAASAATASVAVLATLQIKTTPKTRNR
                     EPVNVNSMLAGFRYIWQEKLIFGSISLDLFAVLLGGAVALLPVYAREILRTGPWGLGL
                     LRSAPALGAATMALTLAYRPMRRNAGRTMLWCVAGFGAFTVLFGISHNLVLSLIALVF
                     VGATDMVSVIVRGTLIQVATPDEMRGRVNAVDMVFIGASNEFGQFESGLTAHWFGTVP
                     AVVLGGIGTLVVTALWAWGFPELRNADRLTDIRRDPGGARAG"
BASE COUNT          302 a          379 c          349 g          221 t
ORIGIN      
        1 aactacggcc ccgcccatgc ctcttgaaag aacattcgca cacatggggc tgaacgcgta
       61 cctgaagaaa ttgccgcatc cggagccgca ggtgcccgag aatgaagcga gttacatgcc
      121 cggagctcaa gtgtacaaag agaattgcgc ggtatgccat ggtctgcctg accaacccaa
      181 gaccaacatt gccgaaggca tggctcccgg cccaccccag cttttcaaag gcaagggcgt
      241 cacggatgac gaagcctggg aaacgtattg gaaggtcgaa agcggtattc gcatgaccgg
      301 catgcccggc ttcaaaggaa agctgaccga accgcagatc tggcaggtca gtgtgctggt
      361 gaaaaacgcc gataaaattt cgcccgctgt gaaagccgcg ctgctgagca gcgagccatc
      421 cgcaacgccg gtgattcctg caaaccccac tcacgtgccg ccaacgcgtt agccggcgcg
      481 ggcgccgccc ggatcgcggc gaatgtctgt tagacggtca gcgtttcgca attccgggaa
      541 gccccaggcc cacaaggcgg taactaccaa tgtgccgatt ccacctaaca ctaccgctgg
      601 cacggtgccg aaccagtgcg cggttaatcc tgattcaaat tggccaaact cattggacgc
      661 gccgatgaaa accatgtcca cggcgttcac gcggccgcgc atttcgtcag gcgtggcaac
      721 ttgaatcaat gtgccgcgca cgatgacgct taccatgtcc gtggcgccga cgaaaaccaa
      781 agcaatcagc gacagcacca ggttgtgcga gattccaaac aacacagtaa acgcaccgaa
      841 gcccgcaacg caccacaaca tcgtgcgtcc cgcgtttctc cgcatgggcc ggtatgccag
      901 agttagcgcc atcgtggctg ctcccaatgc tggcgcgctg cgcagcagac caagtcccca
      961 cggtccggtc ctaagtattt ctcgcgcata aaccggaagt agcgcgacag ctccgccgag
     1021 caacacggca aaaagatcaa gggaaattga tccgaaaatt aatttttcct gccagatata
     1081 tcgaaatcct gcgagcatcg aattcacatt cacgggctcg cggtttcgcg tttttggagt
     1141 ggtcttgatt tgcagtgtgg ccagcacggc gacgcttgct gttgccgcgg acgcggcgta
     1201 tacgaaagca ggtccgtgaa atgccgcgta cgcgacgccg ccgagggcag g
//
LOCUS       DATCBB010000924         1295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_255194, whole genome shotgun sequence.
ACCESSION   DATCBB010000924 DATCBB010000000
VERSION     DATCBB010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255194"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /gene="trpB"
                     /locus_tag="VII37_10125"
     CDS             <1..1133
                     /gene="trpB"
                     /locus_tag="VII37_10125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY5070139.1"
                     /translation="MVPLFELERAYELAKSDTSFQARLADLLKNFAGRPTPLQFAERL
                     TTKLGGPRIYLKREDLLHTGAHKINNAVGQGLLAVKMGKPRVVAETGAGQHGVASATV
                     AAQLGLECTVYMGTDDMARQAPNVSRMRLLGAKVVGVESGSRTLKDAINEAMRDWVTN
                     VRTTHYLLGSVLGAHPYPTMVRDFQSVIGREAREQILAAEKRLPTHLFACVGGGSNAI
                     GLFYNFLRDKEVKMVGIEAGGRGTGSGEHAARLGAAQGFTGARPGVLHGTYTYVLQNE
                     DGQISTTHSISAGLDYPAIGPEHAWLADEKRAEYFAMSDNAALDAARLLCRSEGIIPA
                     LESAHALAGLMERLPSLNKDDLVILNLSGRGDKDMDTYSRLL"
BASE COUNT          288 a          368 c          362 g          277 t
ORIGIN      
        1 tgatggtgcc gctcttcgaa ttggagcgcg cctatgaatt ggcaaaatcg gataccagct
       61 ttcaggcccg gctcgctgat ctactcaaga atttcgccgg ccgacccaca cctttacaat
      121 ttgcggaacg gcttaccacc aagttgggtg gccccagaat ttatctgaag cgcgaagatc
      181 tattacacac cggcgcgcac aaaatcaaca acgctgtagg ccagggattg ttggcagtaa
      241 aaatgggcaa gccacgcgtc gttgccgaaa cgggcgcggg acaacacggg gtagcgtccg
      301 cgacggtggc agcgcaacta ggtctcgaat gcactgtgta catgggcacc gatgacatgg
      361 cgcggcaggc gcccaacgtc tcacgcatgc gcctcctggg cgcaaaagtt gttggcgtcg
      421 agtccggcag ccgcacacta aaagacgcca tcaacgaagc gatgcgcgac tgggtcacca
      481 atgtacgcac tacccactat cttctgggct ctgttctcgg cgcgcatccc tatccaacca
      541 tggtgcgcga ttttcagtcg gtgatcggcc gtgaagcgcg cgagcaaatc ctggccgcgg
      601 aaaagcgttt accgacccat ctattcgcct gcgtcggcgg tggttcgaat gctattggac
      661 ttttctataa tttcctgcgc gataaggaag tgaagatggt tggtattgag gctggcggcc
      721 gcggcactgg ttccggagaa cacgcagcga gattaggcgc agcgcaaggt tttaccggcg
      781 cgcgcccagg cgttcttcac ggcacctata cctacgtact gcaaaacgag gacgggcaaa
      841 tttcaaccac tcattctatt tccgcagggc tcgattatcc ggccatcggt ccggaacacg
      901 cgtggctggc ggatgaaaaa cgcgccgagt attttgccat gtcagacaat gcagcgttgg
      961 atgctgcgcg gttgctttgc cgtagcgaag gaatcatccc cgcgctcgaa tccgctcatg
     1021 ctctagcagg cctaatggaa cgcctgccat ccttgaacaa agacgatttg gtgatcctca
     1081 atttgtcggg gcgcggcgac aaggacatgg acacttattc gcgcctgctc taagcttttc
     1141 cgataataat gagcattgct ggcttgcaac caaaccgcat ttcaaaccgt tttgccacac
     1201 tccgtgccgc tggcgaaatg ggaatcgtcg cgtacattac cgctggcgac ccttcgctgg
     1261 atgctacgct ggaattcgtg tccgctctag aagag
//
LOCUS       DATCBB010000925         3413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_61510, whole genome shotgun sequence.
ACCESSION   DATCBB010000925 DATCBB010000000
VERSION     DATCBB010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61510"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VII37_10130"
     CDS             complement(<1..1434)
                     /locus_tag="VII37_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY5070140.1"
                     /translation="MFSRLPIRFLHAVAALGILAAITFFYREVLRVNQTTVALTFVVV
                     ILAVSTFWGFTVSAFMSVAAMLTFNYFFLPPVGTFTIADPQNWAALFAFLASSLIASQ
                     LATRVRKEADEAQRRRNELERLYTFSQTLLVSGNVITLLNAIPSHIVETFEVGAAALY
                     LENKNKFYYSGGASHFADEELKRATVRDEPLRDPGRSLSFVPVHMGARAIGSLGISGQ
                     MLSRETLDALGSMIAIAFERARAVEALGKTEATREGERLKSALLDSVTHDFRTPLTSI
                     KASVTSLLSNGHAADAQKQELLTVIDEECDRLNQLVGDAAEMAQLDAGEFELDLAPNA
                     MNEIVSAALSYCRTLLGPRTVRMDVPETLPSVRADFNRIRDVLVRLIENADAYSPKDQ
                     PITITAEATGNVVLTSVADQGPGIEDMELGLIFDKFYRGKDQRYLVQGTGMGLPIAKA
                     IIQAHGGTISVASQRDHGSVFSFTLPTD"
     gene            1484..2611
                     /locus_tag="VII37_10135"
     CDS             1484..2611
                     /locus_tag="VII37_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEY5070141.1"
                     /translation="MPKKPEDWLEKARTPEKSKGIFKLFLGYAPGVGKTYSMLSEGIR
                     RHNRGEDVVIGFIETHGRKGVEELVPELETVPRRSIEYKGTMFEEMDVDAIIARHPAV
                     VLVDELAHTNIPGSKHRKRYEDLQELLNAKIDVLSTLNIQHMESLAPTVHSITGVTVR
                     ETVPDWVPLMANETVMVDLTPEALQQRMERGDVYSKHKVHQALHNFFRRGNLIALREL
                     ALRQVAEHVDRSLESYMAAKDIEKNWPVRERLAVCISSNPSGQYLIARGARMARRLDA
                     ELYVVHVDTEAEDQGPRHKSLEANIRFAGDLGAKVVRIKGKSIANTMAGFAREKHVTQ
                     VIFGRAAVKGLRKLFYLWAIHRFLRDAPAVDVHIVTHQDPE"
     gene            complement(2633..>3413)
                     /locus_tag="VII37_10140"
     CDS             complement(2633..>3413)
                     /locus_tag="VII37_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5070142.1"
                     /translation="FAINGLVFVLIGLQLPYVLSGIREYSVRQLVLYGVLFSVIVIVL
                     RLLWMYPGAHLAHFVRSRFLHQNNPRPSTKQIFVVGWTGMRGVVALAAAISLPETLAN
                     GSEFPERNLIVFLTFSVIFVTLVLQGLTLPRLIRMLGLAEGHGSDADEEQEARRIITS
                     TALAHLEEARGQDVSDFDSVYDDIARRYARRLASLTKEGSEGDAMSNKELQRYRTILG
                     ELLPLERKTAVRLRNEGRINDEVLRKIEHELDLSESRLALS"
BASE COUNT          809 a         1053 c          852 g          699 t
ORIGIN      
        1 atcggtgggc agagtaaaag aaaagaccga gccatgatcg cgctggctgg cgacactgat
       61 cgttccgccg tgcgcttgaa taatggcttt ggcgatcggc aatcccatcc ccgttccctg
      121 aaccaggtac cgctgatcct tcccgcgata aaatttgtca aagatcagtc ccagctccat
      181 gtcttcgatt cctggccctt gatcggcgac gctggtaagc acaacattcc cggtagcctc
      241 agcggtaata gtaattggct ggtccttcgg tgaataagcg tccgcatttt cgatcaagcg
      301 caccagcacg tcccgaatgc gattgaagtc ggcccgcacg gatggaagcg tctcaggaac
      361 atccatgcgt accgttctcg gccctaacaa agttctgcaa tacgagagcg ccgccgaaac
      421 aatctcattc atcgcattcg gcgccagatc caattcaaat tcgcccgcat ccagctgcgc
      481 catttcggcg gcgtcaccca ccaactggtt caggcgatcg cattcctcgt cgatcaccgt
      541 cagcagttcc tgtttttgtg catccgccgc gtgcccgttg gatagaaggc tcgtcaccga
      601 agccttaata gatgttagcg gcgtgcgaaa atcatgagtc acggaatcca gtagagccga
      661 tttcaagcgt tcgccttcgc gagtggcctc tgtttttccc agtgcttcca ccgctcgggc
      721 ccgctcgaaa gcaatggcaa tcatggaacc cagcgcgtcc agcgtttctc gcgacaacat
      781 ttgacctgaa attcccaggc tcccgatcgc ccgcgccccc atgtgtaccg gaacaaaaga
      841 caaactccgc cctggatccc gcaacggctc atcccgcacc gtcgctcgtt ttagctcttc
      901 gtccgcaaag tgcgatgccc cgcccgagta ataaaacttg tttttgtttt caagatagag
      961 tgccgccgct cccacttcaa atgtttccac gatgtgactg ggaatggcgt tgagcaaggt
     1021 aattacgttc ccggatacca gcaatgtctg gctgaacgtg taaagcctct ccagttcatt
     1081 gcgccggcgt tgcgcttcat ccgcttcctt gcgcacccgt gtcgcgagct ggctggcaat
     1141 cagcgacgac gccagaaacg caaacagcgc tgcccaattt tgcggatccg cgatggtgaa
     1201 cgtcccgacc ggcggaagaa agaaatagtt gaaggtcagc atcgcggcta cggacatgaa
     1261 cgccgaaacc gtgaagcccc aaaaggtcga aactgctaaa atcaccacca caaatgtaag
     1321 cgctacggta gtttgattaa ctcgcagtac ctctcggtaa aaaaaggtaa tcgccgccag
     1381 gatgcccagc gctgcaacgg cgtgcaggaa gcgaatagga agacgtgaga acaccgctgt
     1441 attctagctc agccgctcat acctggatgg tgtaagaccc gcgatgccga aaaaacctga
     1501 agactggttg gagaaggcgc gcaccccgga aaaatccaag ggcatcttta aacttttcct
     1561 cggctatgcg ccgggcgtgg ggaaaaccta cagcatgttg agcgaaggca tccggcgcca
     1621 caaccgcggc gaagacgtag tcatcggctt catcgaaact cacggccgca aaggtgtcga
     1681 ggaactcgtg ccggagctgg aaaccgtgcc tcgtcgcagc atcgaataca aaggcacgat
     1741 gtttgaagag atggacgtgg atgccatcat cgcccgccat cccgccgtcg ttctggtcga
     1801 cgagctggcc cacaccaaca ttcccggcag caagcatcgc aaacgctacg aggacctcca
     1861 ggaacttctc aacgccaaaa tcgatgtgct ctccactttg aatattcagc acatggaaag
     1921 cctcgcaccc acggtccaca gcatcaccgg cgtcaccgtg cgcgaaacgg ttcccgactg
     1981 ggttcctttg atggccaatg aaacggtgat ggtggacctc accccggaag ccctccagca
     2041 gcgcatggaa cgcggcgacg tttactccaa acacaaagtc catcaggcgc tgcacaattt
     2101 ttttcgccgc ggcaatttaa tcgctctgcg cgaacttgcc ctccgccagg tcgccgaaca
     2161 cgtcgatcgc agcctggaat cctacatggc tgccaaggac attgaaaaaa actggcctgt
     2221 gcgcgaaaga ttggccgtct gcatcagttc aaatccctct ggccaatact tgatcgcccg
     2281 cggcgcccgc atggctaggc gcctggatgc cgagctctac gtggtccacg tggacaccga
     2341 ggcggaagac caaggcccga ggcacaaatc tctcgaagcc aacattcgct tcgccggcga
     2401 tctgggagca aaagtggtgc gaataaaagg taaaagcatc gcgaacacca tggcgggatt
     2461 tgcgcgcgaa aagcatgtga cacaagttat ctttggccgc gccgcggtga aaggcttgcg
     2521 caaacttttc tacctatggg ccatccaccg ctttctgcgc gacgctcccg ccgtggatgt
     2581 tcacatcgtc acccaccagg atccggagta acgatcttgc gccccgctta actcaactca
     2641 acgccaggcg cgattcgctc aggtctagct catgttcgat cttgcgcagc acttcgtcat
     2701 tgatgcgtcc ctcatttcgc agccgaacgg cggtttttcg ctctaacggc agaagctctc
     2761 ccagaatcgt gcggtagcgt tgcaactcct tgttgctcat cgcatcaccc tcgctgcctt
     2821 ctttggtcag gctcgccagc cgccgcgcat atctacgcgc aatgtcgtcg tacaccgagt
     2881 caaagtccga aacgtcttgt cctcgtgctt cttccagatg cgcaagcgcc gtgctggtaa
     2941 taattctgcg cgcctcttgt tcttcgtcag catcgcttcc gtgtccttca gccagtccta
     3001 acatacgaat caaccgcggc agcgtcagcc cctgcagcac gagggtcaca aaaatcacgc
     3061 tgaaggtcag aaacacaatc aaatttcgct ccgggaattc gctgccattg gccaatgttt
     3121 caggcagcga aatagctgcc gccagcgcta caactccgcg catccccgtc caccccacaa
     3181 caaaaatttg ttttgtggat ggccgcggat tattttgatg cagaaaccgc gaacgtacga
     3241 agtgcgcgag atgcgccccc ggatacatcc acaaaagtcg caacacgatc acgatgacgc
     3301 tgaacaaaac tccgtacaac actagttgcc gcacgctata ttcccggatc cccgacagca
     3361 catatggcaa ttgcaacccg atcagcacaa ataccaaacc attgatcgca aac
//
LOCUS       DATCBB010000926         1368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_61516, whole genome shotgun sequence.
ACCESSION   DATCBB010000926 DATCBB010000000
VERSION     DATCBB010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61516"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VII37_10145"
     CDS             complement(<1..397)
                     /locus_tag="VII37_10145"
                     /inference="COORDINATES: protein motif:HMM:NF020060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070143.1"
                     /translation="MATVKTALNGKSLQTGTSMETNSSSTGTGLFFQRYFTDGKQSPF
                     DMVEWEQRTALIGNEKGVTIFRQEGVEVPKSWSQTATNIVTSKYFHGKPGSPEREGSV
                     RQLIARVVNTIVRWGEEGGYFADDNSRDAF"
     gene            935..>1368
                     /locus_tag="VII37_10150"
     CDS             935..>1368
                     /locus_tag="VII37_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEY5070144.1"
                     /translation="MGRARLFSMAAFILLVPMCVRAQEKPQAITPSVMSSAYGDKLKI
                     NGIPNPGKINDSLYRGAQPNAQGLEELKKLGITTIIDLRAEDRPKSEWEKKVAERLGI
                     HFVQIPVAGFAAPTNEEVVQFLSLARDPQQKIFVHCLLGEDRT"
BASE COUNT          342 a          370 c          360 g          296 t
ORIGIN      
        1 gaaaagcgtc gcgcgaatta tcatcggcga aataaccgcc ttcttcaccc cagcggacaa
       61 tggtgttcac cacacgcgca atcaactggc gaacggagcc ttcacgctcc ggcgacccgg
      121 gcttgccgtg aaaatattta ctggtgacga tgttggtagc agtctgcgac cagctcttgg
      181 ggacttcaac gccttcctgt cggaaaatgg tcacgccctt ttcgttgccg atcaaggcgg
      241 tgcgctgttc ccattcgacc atatcgaagg gagactgctt gccatccgtg aagtaccgct
      301 ggaaaaacaa accggtgccg gtggatgacg aattcgtctc catcgatgtg cccgtttgca
      361 gcgatttgcc gttcagtgca gtcttcacag tggccatcaa ttcctccaac gcgtaaggct
      421 cccgtgggag ggaacccttt aattttactc ctctgcagct cgcaatgcag aggccggtgc
      481 ccaagcgaaa atagcgtgaa acgcgaatgc tctgccatca ggactggtaa atggcgccag
      541 cctcgtgaca ggaaccgcgc ccaaatacgg gcgcggagct gggacttcgc gctcggtttt
      601 ttctgagaac ccggcttggt atcgtccaac tccaaacacg ccgtggcgat cctcgacttt
      661 ggcgcgtttg gggagtgcac gtcccccgaa tctggattcg cagtatcgac ccacgcgaaa
      721 taactgtcaa gcaaaaatag caggtgaaaa gaaaaaagtc gcaaaatgtt ccagtgactg
      781 tgattaatct gtctataagt agtggtacga ttctggtatc gccataggcg tcaaacactt
      841 agaaaaaaat taaattcgcg tgatcgtaga cgcgtcggca acatcagcgt aagctcattc
      901 catccttcta aacacggcca gcatcaaatc aactatggga cgcgctcgtt tattttcgat
      961 ggcggcattc attttacttg tgccgatgtg cgttcgcgca caggaaaaac cacaagcgat
     1021 cacgccttcg gtgatgtcgt ccgcttatgg cgacaagctg aagatcaatg gaatcccgaa
     1081 tcccggcaaa attaatgaca gcctttatcg cggcgcgcag ccgaatgcac aagggctgga
     1141 agaattaaaa aagctgggaa tcaccaccat catcgatttg cgcgcggaag accggcctaa
     1201 atcggaatgg gaaaagaaag tagccgagcg gctggggata cacttcgtgc agattcccgt
     1261 cgccggattc gcggcgccga ccaacgaaga ggtggttcaa tttctttcgc tggctcgcga
     1321 cccgcagcaa aaaatattcg tgcactgcct gctgggcgag gatcgcac
//
LOCUS       DATCBB010000927         3923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_288139, whole genome shotgun sequence.
ACCESSION   DATCBB010000927 DATCBB010000000
VERSION     DATCBB010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288139"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..616
                     /locus_tag="VII37_10155"
     CDS             281..616
                     /locus_tag="VII37_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5070145.1"
                     /translation="MPSSKTEVLQGTLDLLVLKTLDSLGPTHGFGIALRIQQVSKDLL
                     QLNQGTLYPALLRLEQRGLISSKWGVSENNRKAKYYSLTRAGRKQLEQEVESWGRMSA
                     MINRVLRTT"
     gene            619..3288
                     /locus_tag="VII37_10160"
     CDS             619..3288
                     /locus_tag="VII37_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5070146.1"
                     /translation="MREFFRTLVHRLTATFRRRRLEHDLDEELRSHLEMATEINLNKG
                     MSPEDARREALRSFGGIEQTKELYRDRRGLPFLETAFQDLRFGLRMLRRDPGFSVLAI
                     LCLTLGIGANAAVFSWIEGILFRPYPAVSHQERLLALTGTARSEAGNTGLSWPDFIDL
                     RRNCTLFDSFFVSKITGTTLSIGDRAERATGSIVSDNYFDALGVHPILGRGFEPGEDS
                     GRNAHPVTIISYQLWKGRFNGDPQIIGKTQRLDGVLHTIVGVAPEGFYGTFVGWAMQF
                     WVPASMEEVFEDGGYKLEDRDARWIEAFARLKPGVTIEQAQHEVSSVAMRLEADYPAT
                     NRGRSIKLWALWQTPFNNASTLLPTLEIMLAVVVFVLLIACANVGNLLLVRSFARRHE
                     MTVRLAIGAGRGRIVKQLFTEALILSAFGTAGGLLVAHWCRHALVLLFPARGGVAMHL
                     PGEIDWRVLLLSAAVCLLATLLLGLVPAMQTSKIDLAGALKADSAGVVGSRGRAWVRS
                     GLVVVQVSLSFVLLVGAGLLIQSLQKIRTSSPGFSTHGVMFTGVGLVSAAYDAQRAQN
                     FQVQLIDRVKAMPGVESVVFARMTPLSYGSFSSTPIAVDGYDVPPEERPTVEYNEVGP
                     DYFATMGIPLVSGREFTRADDERGALVAIVNETMAAQFWRDRNPIGERVQVKDRWMQV
                     VGVAKDSKYRSVRETAKPFFYVPLRQNFSVSAALFIRTPLSPETVAVTLAREVHALDP
                     SLAPYELITLQEQLDRSTSPQLVAVTLVSVLGALALLLAVIGLYGVMSYAVSQSMREL
                     GLRMALGADASNLVQLVLSRGLALTAGGALLGAAVALALTRLLGNLLYHVSPRDPLVF
                     GAAFAVMTIAALAACFLPAWRATRTDPAEALRD"
     gene            3386..3727
                     /locus_tag="VII37_10165"
     CDS             3386..3727
                     /locus_tag="VII37_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5070147.1"
                     /translation="MQKQLDLLQGTLDMLILKAVSLGPLHGYGILLRIQQVSKEQLEI
                     QQGSLYPALYRLEHQGWITSEWGASENNRKAKFYRLTAIGKRKLQADTENWNRMADVI
                     AGILGATPEEV"
     gene            3724..>3923
                     /locus_tag="VII37_10170"
     CDS             3724..>3923
                     /locus_tag="VII37_10170"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEY5070148.1"
                     /translation="MTIWVRFRSWVNATLRRSRMESDMEDELRFHMQTYADDLLRSGV
                     PQQEAMRRARLQFGAAERVKEE"
BASE COUNT          801 a         1141 c         1108 g          873 t
ORIGIN      
        1 gagctattac tgccccaggg tttaactttt gtagatcgcg gctttagccg tgacaatatg
       61 gctgccttga tttcatcggg cttcagcgcc tgagattcgt cgaagccgcc cgtaccaccg
      121 gaaattgtcc gcgtgcacca caccggggga gcgaccgtag ccctcgccag cctcgccgaa
      181 ggggcgcgcc tgagggtcct cgcgtggcaa aactcctgtt gaccgccaaa atgaatctag
      241 tacactcctt ttttctcatg ttggccgtct aaaggaacat atgccgtctt ctaaaaccga
      301 agtcctgcag ggaaccctgg acttgctggt cctaaaaacg ctcgattccc tcggccctac
      361 ccacggcttc gggattgccc tgcgaatcca acaggtctct aaagatttgc tccaactaaa
      421 ccagggaacc ctatatcccg ccctgctacg cctggaacaa cgcggcctga tctcttccaa
      481 gtggggcgtt tcggaaaata atcgcaaagc caagtattac tcactgactc gcgccggacg
      541 caaacagctc gagcaggaag tggaaagctg gggccgcatg tccgcaatga ttaatcgagt
      601 gttgaggacc acctagccat gcgcgaattt ttccgtacgc tcgtccaccg cctcactgca
      661 accttccgcc gccgccgtct ggaacatgac ctggacgaag aactgcgctc gcacctggag
      721 atggctacgg aaataaatct aaacaaaggc atgagccccg aggacgcccg acgcgaagcc
      781 cttcgcagct tcggcggcat cgagcaaacc aaggagctat accgcgacag aagagggcta
      841 cctttcctcg agaccgcttt tcaagacctc cgattcggct tgcgcatgct ccgccgagat
      901 ccgggttttt ccgtcctcgc gatactttgc ttaaccttag gcatcggcgc aaacgccgcc
      961 gtgttcagtt ggatcgaggg aattttgttt cgtccgtacc cggcggtctc tcaccaggag
     1021 cgcttgttgg ctctcaccgg tacggcacgc agcgaggctg gcaatactgg cctttcctgg
     1081 cctgatttca tcgatctgcg caggaactgc acgctttttg attcattttt cgtgagcaaa
     1141 atcaccggca ctactctcag tatcggtgac cgcgccgagc gcgccacagg aagcatcgtt
     1201 tcggacaatt attttgacgc ccttggggtc cacccaattc ttggccgcgg attcgagccc
     1261 ggcgaagact caggacgcaa cgcccatccc gtaacgataa tcagctatca gctatggaaa
     1321 ggccgcttca atggcgatcc gcaaattatc ggcaagaccc agcgactcga tggtgtgctg
     1381 cacaccatcg tcggtgttgc gcccgaaggt ttctatggga ctttcgtcgg ctgggcgatg
     1441 caattttggg tccccgcgtc gatggaggaa gtgtttgaag acggcggcta caaattagag
     1501 gatcgcgacg cgcgctggat tgaagccttc gcacggctca aacctggtgt cactatcgag
     1561 caggcacagc acgaagtttc gtcagttgcg atgcgtttgg aagccgatta tccggcaacc
     1621 aaccggggcc gcagcataaa gctgtgggcc ttatggcaaa ctccattcaa caacgccagc
     1681 actctgctgc ccacgctcga aatcatgctg gcggtagtcg tttttgttct gctcatcgcc
     1741 tgcgcgaacg tcggcaatct gcttttggtc agatcgtttg cgcgacgcca tgaaatgacc
     1801 gtgcgtttgg ccatcggcgc gggccgcggc cggatcgtga agcagctctt caccgaggct
     1861 ctgatcctgt cagcatttgg cacagccggc ggactcctcg tggcgcactg gtgccgccac
     1921 gcgcttgttt tgctgttccc ggcgcggggt ggcgtggcca tgcatttacc gggggaaatt
     1981 gattggcgcg ttttgctatt aagcgctgca gtctgcctgc tcgccacgct tctgctcgga
     2041 ctggttccag caatgcagac cagcaagatc gatctggccg gcgcgttgaa agccgattcc
     2101 gcaggcgtgg ttgggagtcg gggaagagcg tgggtgcgct caggtctggt ggtggtgcaa
     2161 gtctcgctga gtttcgtttt gttggtaggc gcagggctgt tgatacagag cctgcagaaa
     2221 attcgaactt ccagtcccgg tttttcgacg catggagtga tgttcaccgg tgtcggcttg
     2281 gtatccgcgg catacgatgc gcagcgcgcg cagaattttc aagttcagct cattgaccgc
     2341 gtcaaggcca tgccaggcgt tgagtcggtg gtttttgcgc gaatgacgcc gctcagctat
     2401 ggaagttttt cttccacgcc aattgccgtg gatggctacg atgttccacc cgaggaacgc
     2461 cccaccgtgg aatacaacga agtggggccg gactatttcg cgaccatggg catccctttg
     2521 gtttctggcc gcgaatttac tcgtgcagac gacgaaagag gcgctctggt agccatcgtg
     2581 aacgaaacaa tggctgcaca gttttggcgc gacagaaatc cgattggcga gcgtgttcaa
     2641 gtcaaggacc gctggatgca ggtagtcggc gtagcgaaag attctaagta ccggagcgtt
     2701 cgcgagacag caaagccctt tttctatgta ccactgcggc agaatttctc cgttagtgcg
     2761 gcattgttta ttcgaacgcc gctcagcccg gaaacggtag cggtcacttt agcccgcgag
     2821 gtgcacgcgc tggacccgag ccttgccccc tacgaactca tcactctgca agaacagctg
     2881 gatcgctcga catcgccgca actcgtggcc gtaactttgg taagcgtctt gggtgccttg
     2941 gcgctgctgc tcgctgtcat cggattgtat ggtgtgatgt cgtacgcagt atcacaaagc
     3001 atgcgcgagc tcggactgcg catggcgctt ggcgcagatg cttcgaatct ggtgcagttg
     3061 gtgctatcgc gcggcctggc cttaacggcc ggcggcgcgc ttctcggcgc cgctgttgcg
     3121 ctcgcattga cgcgattgct tggaaatctt ctgtaccacg tgagcccacg cgacccactc
     3181 gtgttcgggg ccgccttcgc agtcatgacc atcgcggcgc tggcagcatg tttcctgccc
     3241 gcctggcgcg ccacccgcac cgatcccgcc gaagccttac gcgactgatt tactatccct
     3301 ttggggagcc gcattactcc ttttcccctt gacagtctag gagagtcgcg catacattcc
     3361 tagttgatct aggagagatg gtcgcatgca aaaacagctc gaccttctgc aaggcactct
     3421 cgacatgctg atcctcaagg ctgtttccct cggccctctg cacggctatg gcatcctgct
     3481 gcgtatccag caagtctcca aagaacagct cgaaatccaa caaggctcac tctacccggc
     3541 gctatatcga ttggagcatc agggctggat caccagtgaa tggggcgcgt cggaaaataa
     3601 tcgcaaagca aagttttatc gcctcaccgc gatcggcaaa cgcaagctac aagccgatac
     3661 cgaaaactgg aatcgcatgg cagacgtaat cgctggaatt ctcggagcca cgccggagga
     3721 agtgtgacca tctgggtccg ttttcgttct tgggtcaacg ccacgttgcg ccgctcgcgc
     3781 atggaaagcg acatggaaga cgaactccgt ttccacatgc agacgtacgc cgacgatctg
     3841 ttacgcagcg gcgttccaca gcaggaagcg atgcgtcgcg ccaggttgca gtttggcgca
     3901 gcagagcgag tcaaagaaga gtg
//
LOCUS       DATCBB010000928         1259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_385224, whole genome shotgun sequence.
ACCESSION   DATCBB010000928 DATCBB010000000
VERSION     DATCBB010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385224"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VII37_10175"
     CDS             complement(<1..425)
                     /locus_tag="VII37_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5070149.1"
                     /translation="MQARPEFNVVVLPKRLPEAELLPIVADATALVVRSETKVTKNVI
                     AAAKQLRVVGRAGVGVDNVDIEAATQHGTVVMNTPGGNTVTTAELSFAMLLSLARKVP
                     QAAATMIAGKWDRKLFQGVELQGKTLGVLGMGRIGTEVA"
     gene            complement(607..930)
                     /locus_tag="VII37_10180"
     CDS             complement(607..930)
                     /locus_tag="VII37_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070150.1"
                     /translation="MLGNSVWGQVFDTDNVTRVEAVKVASQLKVGMREEDASLLLATN
                     ELRNAFGVGAISGWDSCYGLSDGSSLHLSYTAREIAKDGRWEETVFCRKHSYKVTESI
                     SSWLH"
     gene            complement(988..>1259)
                     /locus_tag="VII37_10185"
     CDS             complement(988..>1259)
                     /locus_tag="VII37_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5070151.1"
                     /translation="YGTPPDEDDMAHINCPVYGFYAGNDARVTATVPTATEQMKAAGK
                     TYEPVTYDGAGHGFMRAGEAPDASDANKKARDDAFKRWLDLLKKI"
BASE COUNT          276 a          361 c          322 g          300 t
ORIGIN      
        1 ttggcgactt ccgtgccgat gcggcccatg ccgagcacgc caagcgtctt gccttgcagt
       61 tcgacgcctt gaaacaattt gcggtcccat tttccggcaa tcatcgtcgc ggccgcctgc
      121 ggcactttgc gggcaagtga aagcagcatg gcaaaactca attccgccgt cgtcaccgtg
      181 ttgccgccgg gcgtgttcat cacgaccgtg ccgtgctgcg tggcggcctc gatgtccacg
      241 ttgtccacgc cgacgccggc gcggccgacc acgcgcaact gcttcgccgc cgcgatgaca
      301 ttcttggtca ctttcgtttc ggaacgcacg accagcgcgg tggcgtccgc gacgatcggc
      361 agcagttccg cctccggcag acgtttcggc agaacgacga cgttaaattc cggccgcgct
      421 tgcaacaacg cgatgccttt gggcgagatt gggtcacata caacaatttt cataacagaa
      481 agaaatgtga gtctagggca tggccgggtc gaggtcaaat aacgaatcag gcaaaataaa
      541 gtgatttagc aggttggaag aactgacggg caaaataaaa atgtaatcca agttagggtg
      601 cgttcgtcaa tgtaaccaag acgatattga ctccgttact ttgtaggaat gctttctgca
      661 aaagaccgtt tcctcccaac ggccatcctt ggcaatttct cgagcggtgt aactcaagtg
      721 caggctgctg ccatcagata aaccataaca actatcccat ccacttattg cgccaacacc
      781 aaaggcattt cttaattcat ttgttgccag caacaatgaa gcatcttcct cccgcatccc
      841 gactttgagt tgggaagcga ctttgacagc ctccactctg gtgacgttgt cagtgtcaaa
      901 aacttgcccc cagacggaat tgcctaacat gaattagcta agaggatgat ggttttcatg
      961 ctgcgctccg atattttttc acggtgctta aattttcttc aacaaatcca accaacgctt
     1021 gaatgcatcg tcgcgcgcct ttttgttcgc gtcactcgcg tccggcgctt cgcccgcgcg
     1081 catgaaaccg tggcctgcgc cgtcgtaagt caccggctcg taagttttgc cggcagcttt
     1141 catttgttcg gttgccgtcg gaacggtcgc cgtcacgcgc gcgtcgttgc cggcgtaaaa
     1201 tccatagacc ggacaattga tgtgcgccat gtcgtcttca tctggcggcg tgccgtaaa
//
LOCUS       DATCBB010000929         1017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_320522, whole genome shotgun sequence.
ACCESSION   DATCBB010000929 DATCBB010000000
VERSION     DATCBB010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320522"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VII37_10190"
     CDS             <1..415
                     /locus_tag="VII37_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5070152.1"
                     /translation="PLDKNAARALTDSFKAACGAQGAHVIYDAIGGDYSEAALRAIAW
                     QGRHLVVGFTAGIPKLPLNLPLLKGCEIVGVFWGEFTHRNPALHAANVAALMTLYLDS
                     KIKPAVTERFPLARGGEAIARLGSRTARGKIVVTV"
     gene            438..>1017
                     /locus_tag="VII37_10195"
     CDS             438..>1017
                     /locus_tag="VII37_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5070153.1"
                     /translation="MDLKYNAEEQKFRAEVRAFFDAELPTDIRTKLQLGRRISKDDML
                     RWQKILFRKGWGAPNWPKSFGGTGWDIVQQHIFEEERADACAPGQNPFSLKMLAPVMQ
                     TFGNAAQQKYFLPRILSGEDWWCQGYSEPGSGSDLASLRTSAVRQGDHYIVNGQKTWN
                     TMGQYADWIFCLVRTSSEGRQQQGISFLLIDMK"
BASE COUNT          208 a          288 c          311 g          210 t
ORIGIN      
        1 cccgctcgac aagaatgccg cgcgtgcgct gaccgatagt ttcaaagcag cctgcggcgc
       61 gcagggcgct catgtcattt acgatgcgat tggcggcgat tacagcgagg ctgcactgcg
      121 cgccatcgcc tggcagggcc ggcatttggt ggtgggcttt accgcaggta tccctaagtt
      181 gccgctcaat ctgcctctgc tcaagggttg cgagatcgtc ggtgtgtttt ggggcgagtt
      241 tacccaccgc aatcccgcgc ttcacgcagc caatgttgcg gcgctcatga cgctgtatct
      301 ggatagtaaa atcaagccgg ccgtgaccga gcgatttccg ctggcgcgcg gcggcgaagc
      361 aattgcccgc ctaggttcgc gcacagcgcg cggcaaaatc gtcgtcacgg tttgaagcgc
      421 cctcaagagg aagccatatg gatcttaaat acaatgcgga agaacagaag tttcgcgccg
      481 aagtgcgcgc atttttcgat gccgagctgc ccacggacat ccgcaccaag ctgcagctcg
      541 ggaggcgcat ctccaaggac gatatgctgc gctggcagaa gattctattc cgcaagggct
      601 ggggcgcacc gaattggcca aagtccttcg gcggcacggg ttgggatatc gtgcaacagc
      661 atatattcga ggaggaacgc gccgacgctt gcgcgccggg gcaaaacccg ttttctttga
      721 aaatgcttgc gcctgtgatg cagacttttg gcaatgccgc gcagcagaaa tactttttgc
      781 cgcggattct ttctggcgag gactggtggt gccaggggta ttccgaacca ggctcggggt
      841 ccgatctcgc ctcgctgcgc accagcgccg tgcgccaggg cgatcactac atcgtcaatg
      901 gacagaaaac ctggaacacc atgggccagt acgcggactg gatcttttgt ctggtgcgca
      961 ccagcagcga aggccgccaa caacagggta tttcgttttt gctgatcgac atgaagt
//
LOCUS       DATCBB010000930         1460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_190538, whole genome shotgun sequence.
ACCESSION   DATCBB010000930 DATCBB010000000
VERSION     DATCBB010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190538"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1181
                     /locus_tag="VII37_10200"
     CDS             270..1181
                     /locus_tag="VII37_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5070154.1"
                     /translation="MGAWAIGGAGWSGSMGPQNEDDSVPAIHAALDRGLNWIDTAALY
                     GLGHSEEVVARALKGRTPRPYVFTKCERVWDDKGKIGASLKAASIRRECEDSLRRLQT
                     DVIDLYQIHWPDPDQDIEEGWTELARLKEEGKVRHIGVSNFSVAQMKRAQAIAPITSL
                     QPPYSIVTREIEHEILPYAKSHNIGVIVYSPMSAGLLTGSMTRERVANFSREDWRRNL
                     PNFKEPLLSRNLRIVERLRDIGNRHNRNAGEIAIAWTLDHPAITAAIVGFRSPQQVSG
                     IVGALEFRLNKSEMAEIDQALNRESAT"
BASE COUNT          352 a          429 c          375 g          304 t
ORIGIN      
        1 gctttcaact ttgaaatcgc ccaattgcgc aaactggagt tcctgcgcag gagccggaat
       61 agccgccacg aacgcggcaa gaaagaccaa gatagaaatc ttcatgacag gcatagtcac
      121 ggcctttaac cagatcctct aattcaaaag tttggcgtgc cattataatt ggcgcgcagg
      181 ggcgcggtta aagtctattc ggtcaaaaga ggtgctcgat agaaacgcgt caacttggca
      241 acagcgatct aaaaatcacc gccctcggca ttggcgcttg ggccattggt ggcgccgggt
      301 ggtccggcag catgggcccg caaaacgagg atgactccgt cccggcaatt catgccgccc
      361 tcgatcgcgg cctcaactgg attgataccg ccgcgctcta cggtctcggc cactccgaag
      421 aggtagttgc ccgcgcgctc aagggacgaa cgccgcgtcc ttatgtattc accaagtgcg
      481 agcgagtttg ggacgataaa ggaaaaatcg gcgcctcgct aaaagcagct tccatccgcc
      541 gcgaatgcga agacagtctc cgccgcctgc aaaccgacgt catcgatctc taccagattc
      601 attggccgga tccagatcag gatatcgaag aaggttggac tgaactggcg cgtctaaaag
      661 aagaaggcaa agtccgccac ataggagttt caaatttcag tgtcgcgcaa atgaagcgtg
      721 cacaggccat tgcgcccatt acatcccttc agcctccata ttcgattgtg acccgcgaaa
      781 tcgagcacga aatcttgcca tatgccaaaa gccacaatat tggcgttatt gtttactcgc
      841 caatgtccgc cggactgctc accggctcga tgactcgcga gcgggtcgca aatttttccc
      901 gcgaagattg gcgcagaaac ctccctaact ttaaagaacc tctcctctca cgcaaccttc
      961 gcatcgtgga acggctgcgc gacatcggca accgccacaa tcgcaacgca ggagaaatag
     1021 ccatcgcatg gaccctcgac cacccggcca taaccgccgc aatcgtcgga ttccgcagcc
     1081 ctcagcaagt ctccggaatc gtgggcgctt tagaatttcg ccttaacaaa agcgaaatgg
     1141 cagaaatcga tcaagccctg aaccgggaat ccgccacata aaagcaatcc ggcctaagat
     1201 tcgctcggca tggcatgggt aagttaggat ggtgctggct ccctgagaaa ttgttccctc
     1261 ttggctccgt tcagcctgtt atcccgtgtg gcgcggacgc ctcgtccgcg ctccttatgc
     1321 tcttgagcga tggcccttcc atgcagccga cgtccccatt ccgcgcgctt ttcgcgggat
     1381 ggggtgggag aaccaccgga tcactaacgc tgtattgcag gactcttttt aaactgcttg
     1441 ctgagagtca aggtctgtct
//
LOCUS       DATCBB010000931         1097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_385324, whole genome shotgun sequence.
ACCESSION   DATCBB010000931 DATCBB010000000
VERSION     DATCBB010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385324"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(503..850)
                     /locus_tag="VII37_10205"
     CDS             complement(503..850)
                     /locus_tag="VII37_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070155.1"
                     /translation="MFLSIAIVLSLLPVVAPAAPAHNRPAPRWHGYGFLPGYHQPPNN
                     SLPAYAQKASVLRMARHNRRPWYIDPIPSYYGYDGDWHYFGRPGFYGGRYNVGSFGSC
                     WTRTPIGPIWNCG"
BASE COUNT          265 a          299 c          311 g          222 t
ORIGIN      
        1 ttggaagcat tgaaaacaaa cggaaatttc tgtcactcaa aagcccgtgg taagcagtgg
       61 aaatgcttgg taatttctag ctaggcgatt gttttcccgc aagaattttt ggctggcgac
      121 gcagtgggat tcgaaccagt ctccacgcaa attccctgct aacagggaat tttacaggga
      181 attctgcgat tttgggtctc ttggggctga tttcgacgca agaagctccg gcgccgcagc
      241 cacttcttga gcaattccct acgcgaatta acagggaaaa tattttcaga aacagggaat
      301 ttgttggacg taacagggaa ttttgctgag cgcccgggaa ttttgctgag cgcccctccc
      361 cgagcctgat tccaaactaa taggccctca actcacgccc tggcagtcac gaaatgctgt
      421 ttgcgtacga acggccgctt cgcgccataa gccgccgttg ccggcaaatg cacacgcgcg
      481 cggtctttaa gcaaaagctt aatcagccgc aattccagat cggcccgatc ggcgtccgcg
      541 tccagcaaga gccgaagctg ccgacattgt agcgaccacc gtaaaagcca ggccggccga
      601 aatagtgcca gtcgccatcg tacccgtaat aacttgggat tggatcgata taccaagggc
      661 gccggttgtg gcgcgccatg cgcaaaaccg aagctttctg ggcgtaagct ggaaggctat
      721 tattcggtgg ctgatgatag cccggcagga agccgtagcc gtgccagcgt ggcgccggcc
      781 ggttatgagc cggcgcagcc ggcgcgacga ccggcagcag cgataggaca atagcaatcg
      841 acagaaacac gaggcgcgac ataagcacac catagacggc cggctgttgg gaggcgattc
      901 aaatctgagt gcacagtcca gcgcaactgc ttccggttcg cttgcgaaac gaccgcattg
      961 cgccacaagc ggtcgctctc attcgaagtc gagaacggct gcctgtcttc taatatttcc
     1021 accccacctg gatgggacga atggcaaggt cagtatccaa atgagcacgg ctgatggacg
     1081 gagtgtgccg gcgcgcg
//
LOCUS       DATCBB010000932         1832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_93893, whole genome shotgun sequence.
ACCESSION   DATCBB010000932 DATCBB010000000
VERSION     DATCBB010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93893"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VII37_10210"
     CDS             <1..651
                     /locus_tag="VII37_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5070156.1"
                     /translation="DLYVEYTGTALNAVLNESPTGDSAAVYQRVRQGYGQRFNFEVTD
                     PLGFENTFAMVIRNEDAQQLHLQRMSDLVAVAPKWRVGVGFEFLERSDGFRGWSEWYG
                     LHFAQPPSVMDLGLIYRALQDHKVDIVAGNSTDGVIDSLHLVILEDDRHYFPPYDAVP
                     IVRRATLEKFPQLRGTLAELAGKITGADMRHLNYLVDGEQRDVAAVVREYRQSHNL"
     gene            732..1823
                     /locus_tag="VII37_10215"
     CDS             732..1823
                     /locus_tag="VII37_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070157.1"
                     /translation="MARSFIVSSLLLVVSAALWVPSAAAQINEGSTYGPLPVFEFHSG
                     FWVNLHHTLYQEAKFGASQDSAAAADKDKSPNKNRPALKTSPGAKSPLTTAEQRAWNE
                     ALNYYVANYIDKDLLFTTELILIKNQLGDFDGCDELSGKKKETCNAGLPANLTRVLEG
                     AAPVYRAHQWPTHDLANRRWVMRVAPLVREQGLGLSQRLADIYQTKWPKDKIRVDVCA
                     YANRYGAYTTLDPLRVTISSTDPRNQDPEALEVLFHEASHGIAEPVQQAIIRECRQRE
                     KGIPRDLWHALIFYTTGEVIRPVISTTTTDSTSGRVSTTSYTPYAIREGIYQRGWDGY
                     LKLLTQFWQPYLDGNSTFDDAIARMVSAL"
BASE COUNT          409 a          569 c          466 g          388 t
ORIGIN      
        1 gacctctacg tcgagtacac cggcaccgcg ctcaacgctg tgctgaacga aagcccgacc
       61 ggcgattccg ccgccgtgta ccagcgtgta aggcagggat acgggcagcg cttcaatttt
      121 gaagttaccg acccgctggg ctttgaaaac accttcgcca tggttatacg taacgaggac
      181 gcgcagcagc ttcatcttca gcgcatgtct gacctggttg cggtcgctcc aaaatggcga
      241 gtgggagttg gttttgaatt tctggaacga tccgacggct ttcgcggatg gagcgagtgg
      301 tacggcttac attttgccca gccacccagc gtgatggatc tcggcctgat ttatcgcgcg
      361 ttgcaggatc acaaagtcga cattgtggcc ggcaactcca ccgacggagt catcgactcg
      421 ctgcatctgg tcattctgga agatgaccgc cactactttc ctccctatga cgcagtgccg
      481 atcgtccgcc gagctacgct tgaaaaattc ccgcagcttc gcggcacatt ggcggagtta
      541 gctggcaaaa tcactggagc agacatgcgc cacctcaact accttgtcga cggggagcaa
      601 cgcgacgtcg ccgcggtagt ccgcgaatat cgccaatccc acaatctgta atgatgtgtt
      661 gctaaagaga ccgcacctat cgccgccgga tttgtgcgca cgcgaactta acgctactat
      721 ttccgaatcg catggcccgg tcattcatcg tttcatcatt gttgttggtt gttagcgcgg
      781 cactatgggt tccatccgcc gcggcccaaa ttaatgaagg ttccacctac ggtcccctgc
      841 ccgtctttga attccatagc ggcttttggg tcaatctaca tcacacgctt tatcaggaag
      901 cgaaattcgg cgcttcgcag gactccgctg cggccgccga caaagataaa agccccaaca
      961 aaaatcgtcc ggcactcaag acctctccgg gcgccaagtc ccctctgacc acggcggagc
     1021 agcgcgcctg gaacgaagct ctgaactatt acgtcgcaaa ttatatcgat aaggatttgc
     1081 tgttcaccac cgagcttatt ctgataaaaa atcagctcgg tgatttcgac ggttgcgatg
     1141 aattatccgg aaaaaagaag gaaacctgta acgccggtct ccccgcaaat ctcacccgcg
     1201 tactggaagg cgccgcgcca gtctatcggg cacatcaatg gcccactcac gaccttgcca
     1261 accgccgctg ggtgatgcgc gtcgcgcctc ttgtgcgcga acaggggctg ggcctttcgc
     1321 agcgtctcgc ggatatttat caaacgaagt ggcccaagga taaaatccgc gtcgacgtgt
     1381 gtgcctacgc taatcgctac ggcgcttaca ccacgctcga tcccctgcgt gtcaccattt
     1441 ccagcaccga tccgcgtaat caggaccccg aagctctgga agtgctgttc cacgaagctt
     1501 cgcacggcat cgccgagccg gtgcagcaag ccatcatccg cgaatgtcgc cagcgcgaaa
     1561 aaggtatccc gcgcgacctc tggcacgcgc tgattttcta caccaccgga gaagtcatcc
     1621 gcccggtgat ctccaccaca accaccgaca gcacttccgg tagagtatcc accacgagtt
     1681 acacccctta cgccattcgc gaaggcatct atcaacgggg ctgggacggc tacctcaagc
     1741 tgcttacgca attctggcaa ccgtacctcg atggcaactc cacatttgac gacgctatcg
     1801 cacgcatggt ctccgcgcta taaacagttc cg
//
LOCUS       DATCBB010000933         1429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_126488, whole genome shotgun sequence.
ACCESSION   DATCBB010000933 DATCBB010000000
VERSION     DATCBB010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126488"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..731
                     /locus_tag="VII37_10220"
     CDS             30..731
                     /locus_tag="VII37_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5070158.1"
                     /translation="MEPLIQFRNLEKSYDAGGSKFFVLRRINLDIQAGEFVTIMGPSG
                     AGKSTLLSIIGMLDSAWSGEYFFLGEAVHKLNNKTRNELHKANIGFVFQSYHLIDNLT
                     VFENLEVPLSYKNIPHKERISLVCDALDRFHIVGKKDLFPNQLSGGQQQLVGVARAVI
                     LHPKLLLADEPTGNLHSAQGEEIMQLFKTLNDAGTTIIQVTHSERNASYGNRIIQLKD
                     GWVVSESSPAAVQSA"
     gene            819..>1429
                     /locus_tag="VII37_10225"
     CDS             819..>1429
                     /locus_tag="VII37_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5070159.1"
                     /translation="MKPATQSYRVLLADDQPDIRDALRLLLKREGYETHGVASPADAL
                     AAIESREFDAVLMDLNYARDTTSGQEGLDLLTRIQMLDSTLPVIVMTAWSSVEVAVEA
                     MRRGARDFVQKPWENIRLLSIIRTQIDLRQALRQASRLEAENRLLRAESRPTFLAKAP
                     AMLPVLDLIGRIGPSDANVLITGEHGTGKEVVARTLHALSGRAS"
BASE COUNT          306 a          447 c          384 g          292 t
ORIGIN      
        1 aatccgcaag gcctgctaag aggagcgctg tggaacccct gattcaattc agaaatctcg
       61 agaaatccta cgatgccggc ggaagcaaat tctttgtgct tcgccgcatc aatttagata
      121 ttcaggcggg cgagtttgtc accatcatgg ggccttcggg cgccgggaaa tccacgctgc
      181 tcagcattat cggcatgctc gacagcgcct ggagcggcga atattttttt cttggcgagg
      241 ccgttcacaa gctcaacaac aaaacccgca acgagctgca caaagccaac atcggattcg
      301 tgttccagag ctaccacctg atcgacaacc tgaccgtgtt tgaaaatctc gaagtgccgc
      361 tctcctacaa gaacatcccg cacaaagagc ggatcagcct ggtctgcgac gctctcgacc
      421 gtttccacat cgtcggcaaa aaggatttgt ttcccaatca gctttccgga ggccagcagc
      481 agcttgttgg cgtggctcgc gcggtgatcc tccatccgaa attgcttctt gccgatgagc
      541 ccaccggaaa tcttcattcc gcgcagggcg aagagatcat gcagctcttc aaaaccctga
      601 atgacgcggg caccaccatc attcaggtca cccattcgga gcgcaacgcc tcctacggca
      661 atcgcatcat tcagttgaaa gatggctggg tggttagcga aagctcgccc gccgcagtgc
      721 aatcggcgta acccgttttg gactggcttt ttagagtgcc acaagcacgc ggtccaaggc
      781 tcaggaagct gaattagaat agactcgcct ccgacgaaat gaagcctgct actcagtcct
      841 atcgcgtgct gctcgccgac gatcaacccg acatccgcga tgccctgcgc cttttgttga
      901 agcgcgaagg ctacgaaacg cacggcgtgg cctcgcccgc cgacgctctc gcggccatcg
      961 agtctcgcga attcgacgcc gtgctgatgg acttgaatta cgcgcgcgac acgacctccg
     1021 gccaggaagg tctcgatttg ctcacccgca tccagatgct ggacagcacc ctgcccgtca
     1081 tcgtcatgac cgcgtggagc agcgtggaag tcgccgtgga agctatgcgc cgcggcgcgc
     1141 gcgacttcgt gcaaaagccc tgggaaaata tccgcctgct ttccatcatt cgaacgcaaa
     1201 tcgatttgcg ccaggccctg cgccaagctt cgcggctgga agccgagaat cgcctgttgc
     1261 gcgctgagtc gcgtcccact tttcttgcca aagcgccggc gatgcttccg gtgctcgatt
     1321 taatcggccg cattggccct tccgacgcca acgtgctgat cactggcgag cacggcaccg
     1381 gcaaagaagt cgtggcgcgc actttgcacg cgttgtccgg gcgcgcctc
//
LOCUS       DATCBB010000934         1810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_320664, whole genome shotgun sequence.
ACCESSION   DATCBB010000934 DATCBB010000000
VERSION     DATCBB010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320664"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VII37_10230"
     CDS             complement(<1..217)
                     /locus_tag="VII37_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5070160.1"
                     /translation="MKNDPVDRREFIKTSGSAAAGMMVTAVLPSSGLITSDSRVKRTL
                     KFSVIGVNHDHIYAQVEAARRGGGELVS"
     gene            complement(237..1544)
                     /locus_tag="VII37_10235"
     CDS             complement(237..1544)
                     /locus_tag="VII37_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5070161.1"
                     /translation="MTQSQTAHDQQLQKNSAVTSRPGRVRWTICAMLFVATSINYMDR
                     QVIGLLKPTLQQNIGLTEVNYGYIIAAFQLAYAIGLISAGRLVDRLGSRIGYPLFMGV
                     WSLATIAHALAGSAFGFGVARFFLGLGEAGNFPAALKTVANWFPQRERSLATGILNAG
                     TNVGAILAPATVPWITIHYGWRTAFVITGLLGIPWIVWWFRKYRNPSEHPTLTGAELL
                     HIRSSEQDEIETQIPWFRLLGYRQTWAFAIAKFLTDPIWWFYLFWLPSFFHSRFNLDL
                     SHLGLPLIIVYNVSVIGSIVGGWLPALFRFSANPARARLTAMFICACLVLPIFKAGHL
                     KSEWAAVALLSLAVAAHQGWAANLFTIPSDMFPPSAVGAVVGIGGMLGSVGAVLFSVT
                     VGKVLQLTQSYVTLFAVAATAYLIAMAVIRILAPGLKKVQFTA"
     gene            complement(1589..>1810)
                     /locus_tag="VII37_10240"
     CDS             complement(1589..>1810)
                     /locus_tag="VII37_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070162.1"
                     /translation="RAFDKSNGNLLWETILPFAGNATPATYEVAGRQFVVIAAGGGKD
                     PKSGSGGVYVAFALPKDFAKESSAQQNKH"
BASE COUNT          465 a          483 c          492 g          370 t
ORIGIN      
        1 aagagacgag ttcgccgcca ccacggcgcg cagcttccac ctgagcataa atatgatcgt
       61 gattaacgcc gataacagaa aatttcaggg ttcgcttcac acgtgagtcc gatgtaatca
      121 ggccgctcga tggcagaaca gcggtcacca tcatacccgc agcggcgctg ccggaagtct
      181 tgataaattc acgccggtcg actggatcgt ttttcatttt gatttccctc ggaaaattat
      241 gccgtaaatt gtactttctt aagtcccgga gccaggatgc gaatcacagc catggctatt
      301 agataggcgg tagcagctac agcaaaaagc gtgacatagc tctgcgtcaa ttgcaggact
      361 tttccgaccg tcaccgaaaa aagcaccgcg ccaactgagc ccaacattcc gcctatgccg
      421 acaactgcac caacagcact gggcggaaac atatctgagg gaatggtgaa aagattcgcc
      481 gcccaacctt gatgtgccgc cactgcaagg ctgagcaatg ccaccgcggc ccattcagat
      541 ttgagatggc ccgctttaaa aatgggaagc accagacaag cacaaataaa catcgcggtg
      601 agacgcgccc tggcaggatt agccgagaac cgaaacaacg ctggaagcca gccgcccact
      661 atgctgccga tcactgaaac gttatagacg atgatcaacg gcaagcccag gtgcgacaag
      721 tccagattga agcgcgaatg gaaaaatgac ggcagccaaa aaagataaaa ccaccagatc
      781 gggtcggtga ggaattttgc gatggcgaaa gcccaggttt gtctgtagcc gagaagtcta
      841 aaccagggaa tttgtgtttc aatctcatcc tgttcactac tgcggatgtg cagtaactct
      901 gcgcccgtca gcgttggatg ttcggaggga ttgcgatact tcctgaacca ccacacgatc
      961 cacggaattc caagaagtcc cgtgattacg aatgccgtgc gccagccgta gtgtatggtg
     1021 atccacggaa cggtggctgg agccagaatg gcgccaacgt ttgtaccagc gtttagtatt
     1081 ccggttgcaa gtgagcgctc acgctgcggg aaccagttcg ccaccgtttt aagcgcggcc
     1141 ggaaaattgc cggcttcgcc gaggcctaga aaaaaacgcg ccacgccgaa tccaaatgcg
     1201 gagcccgcca gcgcatgcgc aatggtcgcc aaactccata cccccataaa cagcggataa
     1261 ccaatgcgag aacccaggcg gtctactaaa cggccggcgc tgatcagtcc gatggcgtag
     1321 gccaattgaa atgcggcgat gatgtagcca tagttcactt ccgtgaggcc gatgttttgc
     1381 tgcagtgtgg gcttgagcag gccaatcacc tggcggtcca tgtaattgat ggaggtggcg
     1441 acgaagagca tggcgcagat ggtccaacgc actcggcctg gacgcgaagt tacagcggag
     1501 tttttttgga gctgctgatc gtgggccgtt tgagattgag tcatgggctt ggcaatgttt
     1561 cagaacctac aggcgcgcag gaaggatact aatgcttgtt ctgctgcgcg gaactttctt
     1621 ttgcgaagtc tttcggtaac gcaaaagcaa catacacgcc gcctgagcca gacttgggat
     1681 ctttgcctcc acccgcggcg atcaccacaa actgtcggcc agcgacttcg taggtggccg
     1741 gcgtagcgtt gcctgcaaat ggcaggatcg tttcccaaag caaattgcca tttgatttat
     1801 cgaaagcgcg
//
LOCUS       DATCBB010000935         4983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_385362, whole genome shotgun sequence.
ACCESSION   DATCBB010000935 DATCBB010000000
VERSION     DATCBB010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385362"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VII37_10245"
     CDS             complement(<1..196)
                     /locus_tag="VII37_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070163.1"
                     /translation="MIPTLFKPVAEQLAYLKKGSAEIIRETELQTKLEKSAKTGKPLR
                     VKLGVDPTAPDIHLGHTVVIR"
     gene            complement(213..839)
                     /gene="purN"
                     /locus_tag="VII37_10250"
     CDS             complement(213..839)
                     /gene="purN"
                     /locus_tag="VII37_10250"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY5070164.1"
                     /translation="MKKRIGVLLSGRGSNFEALAESVAAGRIPNAQIAIVIANREGTQ
                     GIERADARGITTRVIPSRGLEREAYDRQVVSVLREHQVDLVCLAGYMRLLSPYFIQCF
                     PQRILNIHPSLLPSFPGLESQRQALEYGVKFAGCTVHFVDENLDAGPIILQAVVLVLD
                     MDTADTLSEKILREEHRIYSEAVKIVLDGRFKIEGRRVIQLAAQPAHQ"
     gene            complement(933..1979)
                     /gene="purM"
                     /locus_tag="VII37_10255"
     CDS             complement(933..1979)
                     /gene="purM"
                     /locus_tag="VII37_10255"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY5070165.1"
                     /translation="MKYADAGVDIAVADDAKQRIRHHASRTFTPGVLGGIGGFGGLFA
                     LDTKKWKEPILVSSADGVGTKLKIAMAMGVHSTVGGDLVNHCINDILVQGAEPLFFLD
                     YLAMGKLEPTVVEQLVDGMSRACRKAGCALIGGETAEMPGFYPAGEYDVAGFIVGVVE
                     RKNMLNGKAVKPGDALIALPSSGLHTNGYSLARKLVFEIAKLRPDTYVAEVGNKIGAE
                     LLKPHLCYAPALKNIVARGGVSALAHITGGGIPGNLPRVLPSGVKAQIDLSSWPVLPI
                     FKYLAKIGKIDGDELLHSFNMGVGMILVVPPAQVKFVEGDLKKRREKFFRIGRIERSD
                     SGKARLAYSGALGL"
     gene            complement(2130..2816)
                     /gene="lepB"
                     /locus_tag="VII37_10260"
     CDS             complement(2130..2816)
                     /gene="lepB"
                     /locus_tag="VII37_10260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY5070166.1"
                     /translation="MKADKKEEVTQHVPVGTKIQREIIAWFWVGLAFLLINGTIGQAR
                     VIPSGSMENTLLIGDHLIMSRIGYDAGVPFTSWHLPLWRNPKRQQVIIFKPPFAPDTP
                     DYVKRVIGLPGDSVDIHDGAVFVNGQKLTEPYAVGASEPYELHTPYKVSDNCYFVMGD
                     NRGNSYDSRFWGCVPRKDIIGTPVMIYMSLDAPSDAWEPGQIRERFFAYANAILHPGS
                     VRWRRLFHIF"
     gene            complement(2806..4323)
                     /gene="purF"
                     /locus_tag="VII37_10265"
     CDS             complement(2806..4323)
                     /gene="purF"
                     /locus_tag="VII37_10265"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY5070167.1"
                     /translation="MNPKLAYADDHFHDECGVFGVFGHPEAANLTYLGLYALQHRGQE
                     SAGIVSSDGHELHLHRAMGEVEEIFQPAVLAKLPGRAAIGHARYSTAGDKALLNAQPI
                     MIDCNKGKVALGHNGNLTNAAEWRRRLEHRGSIFQTNSDTEVIVHLIARSPVRNLSGA
                     IGDALNQVEGAYSLLLLTTDELYAVRDPRGFRPLVLGRVPATETAAEAWMVASETCAF
                     DLLNAQYVREVEPGEMVRISKSGIESIRFAPPKQQQHCVFEHVYFSRPDSIIFGRSVN
                     ESREALGRLLAQEHPADADIVVPVPDSGVPAAVGYALESKIPFRMGLIRNHYIGRTFI
                     EPSQAIRNFGVKLKLNPVRGLIEGKRVVLVDDSIVRGTTSRKIVRMVREAGAKEVHVR
                     ISCPPTISPCYYGVDTPTREELIASSNSPEQIRKFLGADSLGYISLEGLRRAVDDKEG
                     KFCTSCYTGVYPTDLVQLEVRAGRAGAGNGVVGDLNGNSADELAIERPVVVESEG"
     gene            complement(4320..>4983)
                     /locus_tag="VII37_10270"
     CDS             complement(4320..>4983)
                     /locus_tag="VII37_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY5070168.1"
                     /translation="GILGVIEDATNVLKMAFRHDGDTVVLLDGIGAANDSPGAVSSNG
                     SNSRLAREFSSSEYSKSIGGIVAGEPPAIDLSAEKRLQQCLVALASSRSIQSAHDISD
                     GGVAVTVAESCFASTPASGPARKSGDTLGAHISMEAREPAESAVFGECGARAVISLSP
                     ASLNAVLEMARNYGVKAEQIGKVNADGIFRIELNGSAIIEESVESLHDIWAHSLERAL
                     KQ"
BASE COUNT         1128 a         1454 c         1310 g         1091 t
ORIGIN      
        1 ttcggatcac tacggtgtgg cccaggtgaa tgtccggcgc ggtaggatcg acgcccagct
       61 tgactcgcag gggtttacca gttttagcgg atttttcaag cttggtttgc aactcggttt
      121 cacgaataat ttccgcgctg ccttttttta ggtaagcgag ttgttccgcg acaggtttga
      181 atagtgtggg gatcatcgat tgttgtcgcg gctcactggt gagcaggctg ggccgctaat
      241 tgaattacgc gccggccttc aattttgaag cgcccatcca gaacaatttt taccgcttcg
      301 gaatagatgc gatgctcttc tcgcaaaatc ttttccgaca acgtatcagc ggtatccata
      361 tcaagcacca gaacgacggc ctgaagaatt attggcccgg catccagatt ttcatccaca
      421 aaatgcaccg tgcagccggc aaatttcacg ccgtattcga gcgcctgccg ctgagattcc
      481 agcccgggaa aggaaggcag caatgaggga tgaatattca agatgcgctg cggaaaacat
      541 tgaatgaagt acggcgacag caagcgcata taacctgcca ggcacaccaa atcaacctgg
      601 tgttctcgca aaacggaaac gacctggcga tcatacgctt cccgttcgag cccgcgcgag
      661 gggatgaccc tcgtggtgat acctcgcgca tcggcacgct cgattccctg cgtgccttcg
      721 cgattggcga tgacaatcgc gatctgcgca ttgggaattc ggcccgcggc aacgctctcc
      781 gcgagagctt caaaattcga gccgcgcccg ctcagcaata cgcctatacg ttttttcatg
      841 gacgcaaaac gttagcacac tcattcacat cggtaaaatg tgcgcgagga acctgagtgc
      901 aaaaataatt taacggaaga atagcacccg ccctacaaac ctaacgcacc tgaataggcc
      961 aagcgagctt tgccggagtc gctgcgttcg atgcgtccga tgcggaaaaa cttctcgcgg
     1021 cgttttttca gatcgccttc cacaaattta acctgcgcgg gtggcaccac cagaatcatg
     1081 cccacgccca tgttgaagga gtgcagtagc tcgtcgccgt cgattttccc aatctttgcc
     1141 agatatttga agatcgggag caccggccag gacgacagat cgatctgcgc cttcactccg
     1201 ctgggcagca cacgggggag gtttccagga atgccgccac cggtaatatg cgcgagcgcg
     1261 gatacacctc cgcgcgccac aatatttttt agcgccggag cgtagcacaa atgcggtttc
     1321 aacaattctg cgcctatctt gttccccacc tctgcaacgt aggtgtccgg cctcagtttc
     1381 gcgatttcga ataccaattt gcgcgccaga gaatacccat tggtgtgcaa gcccgaagac
     1441 ggcaacgcaa tcaaggcatc gccgggcttc acggcttttc cgttgagcat attcttgcgc
     1501 tcgacgacgc ccacgatgaa acccgcgaca tcatattcgc cggccggata gaatcccggc
     1561 atctccgcgg tttcacctcc gattaacgca cagccggctt tgcggcacgc gcggctcatg
     1621 ccgtcgacaa gttgctccac aaccgtcggt tcgagcttgc ccatcgccag gtaatcgagg
     1681 aaaaaaagcg gctcggctcc ctgaaccagg atgtcgttaa tgcagtgatt cacaagatcg
     1741 ccgcccacgg tggaatgcac acccatggcc atcgcgattt tcaatttggt tcccacgccg
     1801 tcggcgctgg aaacaagaat cggctctttc cattttttgg tgtcgagggc aaacaggccg
     1861 ccgaatccgc cgatgcctcc aagaacgcct ggcgtgaacg tacggctggc gtggtggcga
     1921 atcctctgct tcgcgtcgtc ggctacggcg atgtcgactc cagcatctgc gtatttcatg
     1981 aaacccttat ctgcaattcg aatattcggc gcgaaatttt gctacaaaat cttaagcacc
     2041 cacaagcaaa tcctggtaac gggttttgac gattacctgc ggaggatgca gcggaaatct
     2101 caccgtggaa ggatgaggaa ttcccgcgat caaaatatat gaaacaatct tcgccagcgc
     2161 acgctaccag gatgcagtat ggcgttggca tacgcaaaaa aacgctcgcg aatctgcccc
     2221 ggctcccagg catccgatgg cgcatccaat gacatgtaaa tcatcactgg cgtcccgata
     2281 atatccttgc gtggcacgca gccccagaag cggctgtcat aagagttgcc gcggttgtcg
     2341 cccatcacaa aataacaatt gtccgaaact ttatacggcg tgtgcagttc gtagggctcg
     2401 ctggctccca cggcataggg ctcagtgagc ttttggccgt ttacaaacac cgctccatcg
     2461 tggatatcta cgctgtcgcc cggcagtccg attacgcgct tcacataatc cggcgtatcc
     2521 ggcgcaaacg gcggcttaaa gataattacc tgctggcgtt tcggattccg ccataacggc
     2581 aaatgccagc ttgtgaacgg aacgccagca tcgtagccga tgcggctcat gatcaggtgg
     2641 tcgccgatca acaaagtatt ttccatggag cccgacggaa ttacgcgcgc ctgtccaatg
     2701 gttccattaa tcagcaaaaa tgccaggccc acccaaaacc acgcgataat ctcgcgctga
     2761 atttttgtgc ccactggcac gtgctgcgtc acttcttctt tcttgtcagc cttcactttc
     2821 caccaccaca ggcctttcta ttgcgagttc atcagcggag tttccattca ggtccccgac
     2881 cacgccgttg cccgcgccgg ctctacctgc gcgaacctcc agttgcacga gatctgttgg
     2941 ataaacgccc gtataacagg aagtgcaaaa ctttccttct ttgtcgtcta cggcccgacg
     3001 caagccttcg agagaaatgt aaccaagaga atcggcccca agaaatttgc gaatctgttc
     3061 cggcgagttg gacgacgcga ttaactcctc gcgcgttggt gtgtccacgc cgtaatagca
     3121 aggtgaaatc gtcggagggc aactgatgcg cacgtgcact tccttggcgc cagcctcacg
     3181 caccattcgc acgatcttgc ggctggtggt gccgcgaacg atggagtcgt caaccaacac
     3241 cacgcgtttg ccttcgatca atcctcgcac cgggttgagt ttcagcttta cgccaaaatt
     3301 cctgatagct tgcgacggct cgataaaagt tcgaccgata taatggttgc gaatcagccc
     3361 catgcgaaaa ggaatcttag actcgagagc gtaacccacg gctgcgggaa cgccggagtc
     3421 cggcaccggc acgacgatgt ccgcgtcggc aggatgttcc tgagcgagca ggcggcccag
     3481 cgcttcacgg ctctcgttca ccgagcggcc gaaaataata ctgtctgggc gagaaaaata
     3541 gacgtgttcg aatacgcaat gctgttgctg tttgggcggc gcaaagcgaa tggactcgat
     3601 gccggatttg gagatgcgca ccatctcccc gggttcgact tcgcgaacgt attgcgcgtt
     3661 gagaagatca aatgcgcaag tttcggaagc caccatccag gcttctgcgg cggtttccgt
     3721 cgccggtact cggccaagca ccaaggggcg aaacccgcgc gggtcgcgca ccgcatacaa
     3781 ctcatcagtc gtcagcaaca acagagagta agcgccttcc acctggttga gtgcgtcacc
     3841 aatggcacca gacaaattgc gcaccggcga tcgggcgatc aagtgaacga tcacttccgt
     3901 gtcgctgtta gtctgaaaaa tcgagccgcg atgttccagc ctgcggcgcc attccgcggc
     3961 gttggtgagg ttgccgttgt gtcccagcgc gactttgcct ttgttgcagt cgatcatgat
     4021 cggctgcgcg ttgagcaatg ctttgtcgcc ggccgtcgaa taccgcgcgt gaccgatcgc
     4081 cgctctaccc ggaagttttg ccagtaccgc gggttgaaaa atttcttcca cctcgcccat
     4141 cgcgcggtgc agatgcagtt cgtggccgtc gctggaaact atcccggcag attcctggcc
     4201 gcgatgttgc aaagcgtaca aaccgagata ggtcaggttg gcggcttcag gatggccaaa
     4261 cacgccgaat actccacatt cgtcgtgaaa atgatcgtcg gcgtaggcga gcttaggatt
     4321 cattgtttga gtgcgcgctc cagcgaatgg gcccagatat cgtgaagtga ttcaacggac
     4381 tcttcgatta tggcacttcc attgagttcg atgcggaaaa tgccatcggc gttaaccttg
     4441 ccgatttgtt cggccttaac accgtagttt cgcgccattt ccagcacggc atttagcgaa
     4501 gcaggggaaa gggaaattac cgcgcgcgcc ccgcactctc caaacacggc ggactccgcc
     4561 ggctcgcgtg cttccatgct gatgtgtgcg ccgagagtat cgcctgactt tcgcgcaggc
     4621 cccgacgcag gcgttgaggc gaaacacgat tctgccaccg tgaccgctac tcccccgtcg
     4681 ctgatgtcat gagcagattg aattgagcgc gaagacgcca gcgccaccag gcattgttgg
     4741 agacgtttct ccgcagaaag atcgatggcc ggcggctcgc ccgcgacaat cccgccaata
     4801 ctcttggaat attccgacga ggaaaattct cgcgctagac gggaattcga tccgttcgaa
     4861 ctcacggcac ccggcgaatc attcgctgct ccgatgccat caagcaaaac cactgtgtct
     4921 ccatcgtggc gaaacgccat cttcaacaca ttcgtcgcgt cttcgatgac tcctaaaatt
     4981 cca
//
LOCUS       DATCBB010000936         1577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_159928, whole genome shotgun sequence.
ACCESSION   DATCBB010000936 DATCBB010000000
VERSION     DATCBB010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159928"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..>1577
                     /locus_tag="VII37_10275"
     CDS             423..>1577
                     /locus_tag="VII37_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY5070169.1"
                     /translation="MRRLPLFLVAALLLAASLPACATVFATVHGVVHDPGHRPVVGAE
                     VTLQAADSDFALHATTGADGAFEMTQAPIGVYRLTVAAPGLATVTQTLTVASGTNPVL
                     HIPLAVGGSTQSVVVNGSASAADTVTPATLITRAMIDQTPGASRTLGMEMITDYVPGA
                     YMSHNMLHIRGGHETSWLIDGVAIPNTKIASNVGPQIDPKDIDSLETQRGSYAADVGD
                     RTDGVFDVLPRNGFERDREGELLVSAGNFFTGETGISLGDHTEKTAWYASVNGARANY
                     GLATPVAAIYHDATNSQSVFASLIRNQTTKDQLRVNAQYRQDYFQIPYDQDASDWEQA
                     SQYYESFGLRDGQLEHDSFAIFDWTHTISAKAEFSVAPFYHFNQANYDSRA"
BASE COUNT          296 a          417 c          518 g          346 t
ORIGIN      
        1 cggcgggacc ttgtacgcca agacatgcgc tggcggcggt tgttgagagc ctgccggctc
       61 tgatggcgcg ggcgcactcg gctactcgaa gccgcggcga tgcgcgggta agaccaattc
      121 gtacccacgc cggacgcgga cctcctgctt cgatcctgag caaaaatctt cattgaatgc
      181 cgttgtcccg gttttgcggg gtaatgggct aaaacctttt ccttgctgag cggacgagtg
      241 ctatgaccgc atgaatttgt gcttcgcagt tgaggttcgt gctgtcccag gtcccaaaat
      301 cgggacgctg gggcacccag cattggtaca aaaatgaacg gtcagagacc tagttgcgcg
      361 ctgctgtttg cgggttgcga tggtgacttg cgtttgagtt ggcttttccc ttgaggtcgt
      421 tcatgcgtcg tcttccttta tttttggttg cggctttgct gctggccgct tcccttcccg
      481 cttgcgctac ggtgtttgcc actgtgcatg gcgtggttca cgatcctggg catcggcctg
      541 tggttggcgc ggaggtgacg cttcaggcgg ccgattcgga ctttgcgctg catgcaacga
      601 cgggggccga tggcgcgttt gagatgacgc aggcgcccat cggtgtgtat cggctgaccg
      661 ttgcggcacc gggacttgcg acagtaacgc agacattgac cgtggcttcg gggacgaatc
      721 cggtgctgca cattccactt gctgtgggcg ggtctacgca atcggtggtt gtgaatgggt
      781 cggcgagcgc agccgatacg gtgacgcctg caacgctgat tacgcgcgcg atgatcgacc
      841 agactccggg agcgagccgc acgctgggca tggagatgat taccgactac gtacccggcg
      901 cgtatatgtc gcacaacatg ctgcacattc gcggcggaca cgagacgagt tggctgatcg
      961 acggcgtggc gattccgaat accaagattg cgtcgaacgt gggaccgcag atcgatccga
     1021 aggatattga ttcactagag acgcagcggg gcagctatgc agcggatgtg ggcgaccgca
     1081 ccgatggcgt attcgacgtg ctgccgcgca acggattcga gcgcgaccgc gagggcgaac
     1141 tgctggtgtc ggcggggaac ttttttacgg gcgagaccgg aatctcgctg ggcgaccaca
     1201 cggagaagac ggcttggtat gcgagcgtga atggcgcgcg cgcaaactat gggctggcca
     1261 cgcccgtcgc ggcgatctat cacgacgcga cgaattcgca gagcgtgttt gcgtcactga
     1321 ttcgcaacca gacaacgaag gatcaactgc gagtgaatgc gcagtatcgg caggattatt
     1381 ttcagattcc atacgaccag gacgcgagcg attgggaaca ggcgagccag tattacgagt
     1441 cgtttggatt gcgcgacggg cagttggagc atgattcgtt tgcgattttt gactggacgc
     1501 atacgatttc tgcgaaggcg gagttttccg tggcgccgtt ctaccacttc aaccaggcga
     1561 actacgattc acgcgca
//
LOCUS       DATCBB010000937         1078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_449731, whole genome shotgun sequence.
ACCESSION   DATCBB010000937 DATCBB010000000
VERSION     DATCBB010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449731"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VII37_10280"
     CDS             complement(<1..487)
                     /locus_tag="VII37_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070170.1"
                     /translation="MLIALAACAANIFVQGSALADDAQENKALKEQMRQMMQRMDTLQ
                     KQVEALSRQQAATPPPAVVVVPPPVTVMPPPVVAKAAKETPAEPLFDKFIKGFYGTLD
                     ASVDGSTKGMDGMVAYHYGSTQVNGVFPNNGAKGAPGNVPFGPVNWQPSIATNSATLG
                     LR"
BASE COUNT          228 a          309 c          291 g          250 t
ORIGIN      
        1 cgcgcaagcc cagtgtggcc gaattagtgg cgatcgacgg ctgccaattc acgggtccga
       61 acggcacgtt gccgggcgcc ccttttgccc cattgttggg gaagacgccg ttgacctggg
      121 tactgccata gtgatacgca accattccat ccatgccctt ggtggaaccg tctacggacg
      181 cgtccaatgt tccatagaaa cctttgatga atttgtcgaa cagcggctct gcaggtgttt
      241 ccttcgcagc cttcgcgacc accggcggtg gcatcaccgt caccggcggc gggacgacca
      301 ccaccgcggg aggcggcgtc gcagcctgct gcctcgacag cgcttcgact tgcttctgca
      361 acgtatccat acgctgcatc atctgccgca tttgttcttt gagcgccttg ttttcctgcg
      421 catcatcggc caaggcgctg ccctggacga agatattcgc ggcgcatgct gccagcgcaa
      481 tcagcatagg cctcgccagt cttagattta gcatcttgtg atcctcataa aggtcgtttg
      541 atcgaatcga tgatctaaaa atccactgcc acttcgggcg cgcggaggtg attgagcgat
      601 tgaaacaaca aaacgcggct gcgctttgat tgacctcatt cagtaccccc tttcagacca
      661 actcagcaga cggagtgaca caccgcttca cgtagttttc gtggtatacc atatatgatt
      721 ggctttgcga aacaaagtgt tttttttgca acacagattc aattctcgcc gctcgcaccg
      781 aaaaaagggc tgtatgcgga ggcaaagccc agcgtggccg ggttaggttt cgaaggttca
      841 gagactctgg gcgaaatgcc gcgcgcatgg gctgcaaacg gctcaaggtg aacagcgcgc
      901 agccgagcgc gggcatgacc aggccggaca gatcattcgg gctcttgctg attttgaagt
      961 cttcggtgac gccacggtta ctgtgacagg ccaaaaaatg gggcgttaat taccgcccca
     1021 tttttcgacg actcaatgat cacgccattg cgctaacccg tctgcgtctg tacatttt
//
LOCUS       DATCBB010000938         1111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_159953, whole genome shotgun sequence.
ACCESSION   DATCBB010000938 DATCBB010000000
VERSION     DATCBB010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159953"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VII37_10285"
     CDS             complement(<1..770)
                     /locus_tag="VII37_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY5070171.1"
                     /translation="MDSALLIHRIHFAFTATFHYLFPQLTMGLALLIVVLKTIGLCRN
                     DPVYHEAARFWGKIFGINFVLGVVTGIPMEFQFGTNWSHFSRYAGGVIGQTLAMESMF
                     AFFLESALVGLFLFGEKRLSPRGHWWAAVAVFAGSWLSGYFIIATDAFMQYPVGYYTA
                     ADGSLQLASFWAFLLNPWARWQYAHNMCGAVTTGAFIMTSVGAFYLLSGKFIGHARVF
                     VKVGVIVGCIFCVLQIFPTGDKQGKMVTDLQPVTLAAM"
     gene            complement(838..>1111)
                     /locus_tag="VII37_10290"
     CDS             complement(838..>1111)
                     /locus_tag="VII37_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070172.1"
                     /translation="GDFVVLRSSVNRVFRTSMEVGVKVWVENYRSQESRHVSSAYLTF
                     VAVDINGNRLPVAPVVPETEDEKRRYEGAERRREIRRAETARRKRS"
BASE COUNT          272 a          302 c          314 g          223 t
ORIGIN      
        1 tccatggcgg cgagcgtgac cggttgtaaa tcagtaacca ttttgccttg tttgtcaccg
       61 gttggaaaaa tctgcagcac acagaatatg catcccacaa ttaccccgac tttaacaaag
      121 acacgcgcat ggccaatgaa cttgccggag agcagataga aggcgccgac tgaagtcatg
      181 atgaaggcgc cagtggtgac cgctccgcac atgttgtgag cgtattgcca tcgcgcccac
      241 ggattgagca ggaatgccca gaagcttgcc agctgcaggc ttccgtcggc cgcggtgtag
      301 tagccgaccg gatattgcat gaaagcgtcg gtagcgataa taaaatatcc cgataaccag
      361 gaacctgcaa aaaccgcgac cgcggcccac cagtgcccac gcgggcttag tcgcttttcg
      421 ccgaagagaa ataatccgac cagcgccgac tcgaggaaga aggcgaacat gctttccatg
      481 gctagtgttt gtccaatgac gccgccggca tatcgcgaga aatgcgacca gttagtgccg
      541 aactgaaatt ccattggaat gccagttact acacctaaca caaagttaat gccgaaaatc
      601 tttccccaga agcgcgccgc ttcgtgatag acggggtcgt tgcggcacag gccgatcgtc
      661 ttcagcacca cgatgagcag cgcgagcccc atggtgagct ggggaaagag atagtggaaa
      721 gtggcggtga aggcgaaatg gatgcgatgg atgaggagtg cgctgtccat gagtaaggtc
      781 tcgggcctta gctatcagat tgtgaaacgg cgtggttcca tctgcgccgt ccaactccta
      841 acttcttttc cggcgagcgg tttctgcccg acggatttcg cggcggcgct cggccccttc
      901 atagcggcgc ttttcatctt cggtttccgg tacgacgggg gcgaccggca agcggtttcc
      961 attgatatcc acggcgacaa aggtcaaata ggcggaactg acatggcggc tctcctggga
     1021 acggtagttt tccacccaaa ccttcacgcc aacttccatt gaagtgcgga agacgcgatt
     1081 gacggacgag cgcagaacca caaaatctcc g
//
LOCUS       DATCBB010000939         1786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_255533, whole genome shotgun sequence.
ACCESSION   DATCBB010000939 DATCBB010000000
VERSION     DATCBB010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255533"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VII37_10295"
     CDS             complement(<1..1746)
                     /locus_tag="VII37_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5070173.1"
                     /translation="MTTVADPPEIFSAAEPPSGKAPAVTGSLSRWRSIQVSDQVLIAV
                     LILLAVLCYGNSLFNAFVYDDEQQILQNPYVKSWHYLPQIFTTTVWSFVGAAGTTNYY
                     RPLMTFTFLVLWQAFKDIPFGFHLFNVTLNAAVVVLVFAAGRRLFRDRRVAWVAAALF
                     AIHPVHTEVVDWIASVPELEVTFFVLLTFWFFTQLDKTSWKFQIATVASFALAVLCKE
                     PALMFAPLAIAFEHFVRSDNASTTLVAKMKKYAPLCVVAAAYLLLRVALFGKLAPVLQ
                     HPKITWPQTIYSAFALVAGYTKYLFWPAPLSAFHVFHSSVSPWELPVLAGIAICVAAV
                     ALILYLRKRAPAAAFCILWMGITIAPVLNARWMASNVFTERYLYLPSVGFCWLVAWYG
                     LQLWDRSAAKARWPRRILAAAAIVIALMSVLSIVRRNTIWRDDMTLYTRTLQTDPDAA
                     VIRSNRASLYFDALQYDKALPEWQLALAQKPDNVVTMNALGLLYTRLHRNDEAEAMFQ
                     QAIAAKPLWGDSHFSRALLLQKTGHLPEALVEFKTGVTLSPLSAPAHRWYGEALLENN
                     QRDEAVAQFQQAVELD"
BASE COUNT          450 a          458 c          518 g          360 t
ORIGIN      
        1 atccagctcc acggcttgct ggaattgcgc cacggcctcg tctcgttggt tgttctccag
       61 caaggcttcg ccataccagc ggtgagccgg cgcgctgagc ggcgacaaag tcacgccggt
      121 tttaaattcc accagggctt ctggtaggtg gccagttttt tgaagcagca atgcgcgact
      181 gaagtgtgaa tcgccccaca gcggcttggc tgctatggcc tgctgaaaca tggcttcggc
      241 ctcgtcgttg cggtggagcc gggtgtacaa aaggcccagc gcattcatgg ttacgacatt
      301 gtcgggcttt tgagccagcg cgagttgcca ctccggcagc gccttatcgt attgcagagc
      361 gtcaaaatat aaagatgccc gattgctgcg aatgaccgcg gcgtcaggat cagtttgcag
      421 tgtgcgggta tacagggtca tatcgtcgcg ccagatcgtg ttgcgtcgca cgatacttaa
      481 gactgacatc agcgcaatga cgatagccgc tgcggccaga atgcggcgtg gccaacgtgc
      541 ctttgcggca ctgcggtccc atagttgtag cccataccag gcgactagcc agcaaaagcc
      601 cacggatggc aaataaagat agcgttcagt aaatacgttt gacgccatcc agcgcgcgtt
      661 tagaaccggc gctatggtga tgcccatcca cagaatgcag aaagctgcgg ccggagcccg
      721 tttacgcaaa tagagaataa gtgcgactgc agcaacgcat atggctatgc ctgcgaggac
      781 gggcaattcc caaggcgaaa cactggaatg gaagacgtgg aaggccgaca agggcgcggg
      841 ccagaaaaga tatttcgtgt atcccgcgac tagagcaaaa gcggagtaaa tggtttgcgg
      901 ccaggtgatc ttgggatgct gcaagaccgg cgctaatttt ccgaatagcg cgacgcgcag
      961 caataagtac gctgcagcca cgacgcacag tggagcgtat ttcttcattt tcgcaacaag
     1021 cgtagtgctg gcgttatccg agcgaacgaa gtgttcaaaa gcgatggcca acggtgcaaa
     1081 cattaacgct ggttccttgc acaagacagc cagcgcgaat gacgcgacgg ttgctatctg
     1141 gaatttccag gaagttttgt ccagttgggt aaaaaaccag aatgtcagaa gtacaaaaaa
     1201 tgttacttcc agttccggaa cggacgcaat ccaatcaacc acttccgtgt gtaccggatg
     1261 aatcgcgaag agcgcagcgg ctacccaagc cacccgccga tcgcgaaata gccggcgtcc
     1321 agcggcaaat accaacacca ctacggcggc atttaacgtg acattaaaca ggtggaagcc
     1381 gaacggaatg tctttgaaag cctgccagag aaccaggaaa gtaaatgtca tcaacggccg
     1441 atagtaatta gtcgtgcccg cggcgccgac gaacgaccac actgtggtgg tgaaaatctg
     1501 gggcaggtaa tgccagcttt ttacatatgg attttgcagg atttgttgct cgtcgtcata
     1561 cacaaaagcg ttgaagagcg aattcccgta gcaaaggacg gctaggagga tgagtacggc
     1621 gatgaggacc tggtcggaaa cctgaatcga gcgccaccgt gaaagcgatc cagtcacagc
     1681 aggtgctttg ccgctgggcg gctcggcagc gctaaaaatc tcgggtgggt cggctacggt
     1741 agtcattgca aggtgcagac agcatttacg tccgccaagt ctgctt
//
LOCUS       DATCBB010000940         1575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_320951, whole genome shotgun sequence.
ACCESSION   DATCBB010000940 DATCBB010000000
VERSION     DATCBB010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320951"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>1575
                     /locus_tag="VII37_10300"
     CDS             14..>1575
                     /locus_tag="VII37_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070174.1"
                     /translation="MQCFDKPLEQQPTCLMQNSDQKVLDDGHSQSMVAALTSGPSTDL
                     IGAVSTTALAGGGTYSPYVGAIVDLARLMESFRTPDYQYLPSLALPKQEQLNLRLNNP
                     PSFRKPKSVLVVGLPAVEAVQLPPLRPVHAGEVLCLEKPSLVLAVEGAPLVFSTDIGH
                     HFVLEFKDKSGAAVKLPARADAVHGGFLIDVHELEGRELEPEVTGTLRGSWGFEAYEG
                     PRFDLKSSHNGKWNVAAAEQSALVVGRDDVLHVQSGCAVCVEKVSFKNEKGVELKTSW
                     KVLRADTLEVGVGLKDETPGAVTMSVKQFGLAKSDEVALHTYSEAAHLERFTINSGDQ
                     QGVLRGTRLDEVDSVELKGVKFSPAKLSRANEKDELRLAAATAPPANALLPEEKVVAH
                     VALKDARVLDLQTTVEAARPKVTLLSKNVQPGPNPTALRFGSADELPQDARLAFFLKA
                     VIPEKFSHAEKIEVATADEAFHVSLSVADGNLVMQDAENVLATLDPLKSFGASAFGPL
                     RFRPVDAEGAKG"
BASE COUNT          342 a          327 c          548 g          358 t
ORIGIN      
        1 tatcaagttg gaaatgcagt gcttcgataa gccgcttgaa cagcagccaa cgtgtttgat
       61 gcaaaacagc gatcagaaag ttttggatga tggacacagc cagtcgatgg tggcggcgtt
      121 gacctccggg ccgtccacgg atttgattgg agcggtgagc accacggcat tagctggtgg
      181 cggaacgtat agtccgtacg ttggcgcgat tgtggacttg gccaggctca tggaaagttt
      241 tcgcactcct gattatcagt accttccttc tctggcattg ccgaagcagg aacaattaaa
      301 tttgcggctg aataatccgc cgtcatttcg caagccgaaa tcggttctgg tcgtgggatt
      361 gcctgcagta gaagcggtgc aattgccacc gttgcgtccg gtgcatgcgg gcgaggtttt
      421 gtgtctggaa aaaccttctc tggtgctggc ggtggaaggg gctccgttgg ttttttcaac
      481 ggatattgga catcacttcg tgctggagtt taaggataag tctggcgcgg cggtgaaatt
      541 gccggctcgc gcggatgcgg tgcatggcgg atttttgatt gatgtacacg aactggaagg
      601 gcgagaactg gaaccagaag taaccgggac tttgcgcggg tcgtggggat ttgaggctta
      661 tgaagggccg cgatttgatt tgaagagttc gcataacggc aagtggaatg ttgcggccgc
      721 ggaacagagc gcattggttg tggggcgcga tgatgtgttg cacgtgcagt cgggatgcgc
      781 ggtgtgcgtc gagaaggtta gctttaaaaa cgaaaaaggc gtggaactga agacgagttg
      841 gaaagttctg cgtgcggata cgctcgaggt cggcgtggga ctgaaagatg agacaccagg
      901 cgcagtgacg atgtcggtga agcaattcgg attggccaaa agcgatgagg tggcactaca
      961 cacgtattcg gaagcggcac atctggagcg cttcacgatt aattccgggg accagcaggg
     1021 agttttgcgt gggacgcggc tggatgaagt ggacagtgtg gagttgaagg gcgtgaagtt
     1081 ttcgccggca aaattatcgc gcgcgaatga gaaggacgag ttgcggctgg ccgctgcaac
     1141 tgcgccgcct gcgaatgcgt tgctgccgga ggaaaaagtg gtggcgcacg tagcgttgaa
     1201 ggatgcgcgg gtgctggatt tgcagacaac cgtggaggcg gcgcgtccta aggtgacgct
     1261 gttgagtaaa aatgtacagc ccggacctaa cccgacggcg ctgcgttttg gcagcgcgga
     1321 tgaattgccg caggatgcgc ggttggcatt ctttttgaag gcggtgattc cggaaaagtt
     1381 ttcgcatgca gaaaagattg aagtggccac ggcggatgaa gcgtttcacg tttcgctgag
     1441 cgtggcggat gggaacctgg tgatgcagga tgcggaaaat gtgctggcga cactcgatcc
     1501 gttgaagagt tttggggcct cggcgtttgg tccgctgcgg tttcgtcctg tggacgctga
     1561 gggagcgaaa ggcca
//
LOCUS       DATCBB010000941         1689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_320953, whole genome shotgun sequence.
ACCESSION   DATCBB010000941 DATCBB010000000
VERSION     DATCBB010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320953"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VII37_10305"
     CDS             complement(<1..230)
                     /locus_tag="VII37_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY5070175.1"
                     /translation="MTPQDAHLQIGSLLFDGLDQIDLTGPFEVLSRIPNSSYRIYGKS
                     LEPVRDMRGLRLTADALLEDAPQLDLLHIPGG"
     gene            complement(630..>1689)
                     /locus_tag="VII37_10310"
     CDS             complement(630..>1689)
                     /locus_tag="VII37_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070176.1"
                     /translation="ITQYGASEPQSVQLHAFTEVAHLDRFAIHAGDTQGLLKGSRLDE
                     VESLLMNGVDFMPGKFSTSQGGDELQMVTSNTQAVSLLKHGDSAKGKVTLKDGRTIEV
                     IASVDAPRPSVTLLGKSVQPSASSSLSNIQLSNQDELPQDAKLTFSVRARSPATFAHD
                     EQIEVATPDEAASVALSTINGGITLEDAKVAVATLDPAKAFGPSAFGPLQFRVVAGGI
                     AGDWQPLATLVRLPVLTELLCPATAELACKLSGSNLYLLDSVAGGPQFDHAIGVPDGF
                     PGNALPVPHPTNGQLYVKLRDDPSVINLAALAAHELAASADQTTRAVVRHAAAREEPE
                     PVLTPGGAAPEAATPIKK"
BASE COUNT          361 a          492 c          473 g          363 t
ORIGIN      
        1 tatccccccg gaatgtgcag taaatccagc tgcggtgcat cttccaggag cgcatctgct
       61 gtcagacgca gacctcgcat atcccggaca ggttcgagtg acttgccgta gatgcggtaa
      121 ctcgaattcg gaattcgtga caagacctcg aaaggacccg tcagatcaat ttggtccaat
      181 ccatcaaaca gcagcgaacc gatttgaaga tgcgcatctt ggggtgtcat aggagccctt
      241 cttgatccga aaaatacgcg ccttcgcata ccgcattgac cgatgcgggt gtcgtcaaac
      301 gccggcgctc attccatctt tatagaatcc agcgaaaaaa agagtaaatg acgcgttaac
      361 ttgccgtgag tcgtgcagcg ccgcggaccg tgcaagtcat gagtggcgcg acacgcgtcg
      421 agattccgcg acattttgca taacgctgtt acacaagtaa aagataccgt gcggcatgac
      481 agctaaccgt agaatgaaag gtagagtgca tgcggcggcg atcacgcgct cacggtgtac
      541 ggtctcctgc cctagccgct ggcgcatcac tgtttctaat ctcgcgcggc cagcgagtca
      601 accgcggttt gcggtgcgcc ttttgcgctc tattttttga ttggggtcgc cgcttcgggt
      661 gccgcaccac caggagtgag caccggctcc ggttcctcgc gtgcagcagc atgtctgacg
      721 acggcccgcg tggtttgatc cgctgacgcc gccaactcgt gtgccgccag cgccgctaag
      781 ttgattacgg acggatcgtc gcgcagcttc acgtacagct gtccattggt cggatgaggt
      841 acgggcaacg cattacccgg gaagccgtcc ggaaccccta tggcatgatc gaactgcggg
      901 ccacctgcta ccgaatcgag cagatagaga ttcgagccgg agagtttgca ggccaattcg
      961 gctgtcgcag gacatagaag ctccgtgagc actggcaagc gcacgagcgt cgcgagcggc
     1021 tgccagtcac cggcgatgcc ccccgccaca acccgaaatt gcagcggccc aaaggctgaa
     1081 ggtccaaacg ctttcgccgg atcaagtgtt gcgacggcaa cttttgcatc ctcaagcgta
     1141 atgccgccat taattgtgct aagcgcgacc gacgccgctt catccggcgt tgcgacttca
     1201 atctgctcgt catgcgcgaa tgttgcgggc gagcgtgcgc ggacggaaaa ggtcaacttc
     1261 gcatcctgcg gcaactcatc ttggttggac agctgtatat tgctcagact gctcgaagcg
     1321 gagggctgca cgctcttgcc aagcagcgtt acgctcggcc gtggcgcatc taccgaggca
     1381 atgacctcga tagttcgtcc atccttcaga gtgactttgc ccttagccga atcgccatgc
     1441 tttaggagtg agacggcttg tgtgttcgag gtcaccattt gcagctcatc gccaccctgg
     1501 cttgtcgaaa atttcccagg catgaaatca acaccattca tcagcagact ctcgacttca
     1561 tcgaggcggc taccttttag aagtccctgt gtatctccag cgtggatggc gaagcgatcc
     1621 agatgtgcca cctcggtgaa tgcatgcaat tgaacgctct gcggctcgct cgcgccgtac
     1681 tgcgtaatg
//
LOCUS       DATCBB010000942         1389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_126803, whole genome shotgun sequence.
ACCESSION   DATCBB010000942 DATCBB010000000
VERSION     DATCBB010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126803"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..835
                     /locus_tag="VII37_10315"
     CDS             83..835
                     /locus_tag="VII37_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY5070177.1"
                     /translation="MKRKTTRTRLDSLLVAQGLAPNAQKAQAMILAGEVFVNGVRVST
                     SGTPVAEDAMVEINSREQRYASRGGFKLEGALRDFGIDPTGKICLDIGSSTGGFTDCL
                     LQKGAIRVYAVDVTVDQIAWKLRQDVRVVRIERNARELTSRDIPESVELATVDVSFIS
                     AGKVLGPAASVAVEGATFLILVKPQFELDRGEIGPGGIVSDASLHEKAISIVQQAAAS
                     AGLECLGVKPSQLTGAEGNQEYFLHARKISLG"
     gene            924..>1389
                     /locus_tag="VII37_10320"
     CDS             924..>1389
                     /locus_tag="VII37_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY5070178.1"
                     /translation="MPSLLPWLEDRGIHVVYDQETATSLAEPSEGRSREQVAAASDLL
                     LVLGGDGTLLAAASVAAPRGIPILPINMGSLGFLTSFMLEELYPALEDILAGRLTISE
                     RVMLHAELERGDKILDKQTVLNEVVINKGALARMIELELSIDNDFVCRYRADG"
BASE COUNT          324 a          387 c          379 g          299 t
ORIGIN      
        1 gcgcgcctcc cgattgcgcg ctctggcaga atttcttgtg catcgtcgcg cctgatctgt
       61 ttcatgaatg ctgcagctct gcatgaaaag aaagaccaca cggacgcgcc tcgatagctt
      121 gttggtggcg cagggcctcg ctccgaacgc gcaaaaagct caggcgatga ttcttgccgg
      181 cgaagttttc gtaaatggag ttcgagtaag cacctccggc acacctgtcg cagaagacgc
      241 gatggtggaa ataaatagcc gcgaacaacg atacgccagc cgaggcggat tcaaacttga
      301 aggtgctctg cgagattttg gcatcgaccc cactggtaaa atatgtttgg acatcggatc
      361 ttcgactggc ggattcactg actgtttgct gcaaaaaggt gcaatccgtg tctacgcggt
      421 tgacgttacc gtggaccaga ttgcctggaa attgcgccag gatgtgcgcg tcgtacgaat
      481 cgaacgcaat gccagggaac ttacctcgcg cgacatccca gaatcagtgg aactggcgac
      541 cgtggacgta tccttcatat ctgctggaaa agtattggga cccgccgcaa gtgtagccgt
      601 cgagggcgcc acgtttctaa tcctggtaaa gccgcaattc gaattagacc gtggggagat
      661 aggtcctggc gggatcgtca gtgatgcgag cctgcatgag aaagcaattt caatcgtcca
      721 gcaggcagcc gcatccgcag gcctggagtg cctgggcgta aagcctagtc aactgacggg
      781 tgcggaaggt aatcaggaat attttctgca cgcacgcaaa atttcgctag ggtagaatcg
      841 ctctcacaca catgccgccc gcacatacgt ttcgaaatat cggcatcatt tcgcgcccga
      901 ggcgctccaa tctttctgaa gtggtgccgt ccttgctccc ttggctcgaa gatcgcggca
      961 tccacgtagt ctacgaccag gaaaccgcga cctccctcgc agaaccatcc gaaggccgca
     1021 gccgcgaaca agttgcagcg gcctcagatc tattgctggt tctcggcggc gacggaacat
     1081 tgttggcggc agcaagcgtc gccgcaccca gaggcattcc catcctgccc atcaacatgg
     1141 gcagtctcgg ttttctcact agtttcatgt tggaagaact ttatccagct ctcgaagaca
     1201 tccttgccgg gcgcctcacg atcagcgaac gcgtcatgct ccatgccgaa cttgaacgcg
     1261 gcgacaaaat tctcgataag caaactgtcc tcaacgaagt agtcatcaac aaaggcgcgc
     1321 tggcccgcat gatcgaattg gaattgagca ttgacaatga tttcgtgtgc cgctatcgcg
     1381 ccgacggtt
//
LOCUS       DATCBB010000943         3209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_288548, whole genome shotgun sequence.
ACCESSION   DATCBB010000943 DATCBB010000000
VERSION     DATCBB010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288548"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..561
                     /locus_tag="VII37_10325"
     CDS             58..561
                     /locus_tag="VII37_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5070179.1"
                     /translation="MSKMLREVSMKFFGRYGLAATVLGAGVLAAVLLAPANRSIIFAQ
                     TNAGAGEKGEVERGRYLVEEVAKCAECHTPRNERGELKADAWLRGAPIWIKPVAPISN
                     WADRAPALAGFPSFTEEQGERILEKGTGPEGEALRPPMHIYHMNHADAKAIIAYLKSL
                     PRGDRTN"
     gene            complement(611..1672)
                     /locus_tag="VII37_10330"
     CDS             complement(611..1672)
                     /locus_tag="VII37_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070180.1"
                     /translation="MSFSLLFAVVFSLALFTLHAGVDATGSSDKGTLVVVNQAEHKVL
                     LVEPDIRRTFAVIAVGVSGHEAAVSPDARFIYVPIYGNSGVGKPGTDGATIDVIDIAK
                     RQVSGSIQLGPVRPHRAEFGPDGLLYVTAEMSNTVKIIDPAKQQVTGEIPTGESQSHM
                     LVISPDGHRAYTANVGPGNISVLDVPARRLITVIPVAKTIQRISMSPDGSRLFTHDQS
                     APRLAVIDLPDRKKSEGGDDVASYKVSRWIDLPAVPYASTTTPDGKWLLVACMAKHLL
                     LAIDMATLKVAKSFDLPDSPSEILVTPAGTMAFVSCVSAGKIAMLDLRTWQLQEPIVL
                     TAGVDGLAWVRVETGKSVH"
     gene            2045..2623
                     /locus_tag="VII37_10335"
     CDS             2045..2623
                     /locus_tag="VII37_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY5070181.1"
                     /translation="MGVSSAAALPSGYDPNAYATAAPVAPPVVYAQGSVQYAGFWLRV
                     VAYLIDSVVMGVAFMALFIPFAIMTGLTAVLGNIHPGEDPRDVGAVLGGTFFLGLFTV
                     VCLAFLGGWLYHAKMESSSWQATLGKKALKLRVTDLYGARVTFARASGRHFAKLITGL
                     IPLGVGFMLAGLTDRRQALHDMIASCVVLRDG"
     gene            complement(2643..>3209)
                     /locus_tag="VII37_10340"
     CDS             complement(2643..>3209)
                     /locus_tag="VII37_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070182.1"
                     /translation="YTLYPCASCDDLSVVFIDSSTVAFGQLRLLQQLIEVRVAANDSL
                     IQNEKMFPLISQVNGRGIFWGVLNPGGTHQAVRQIVPEAAQFPQAGKLLNKLQGMVIS
                     IQGTADLDVHFQLISSSPDDAATISQLLQAGILLRQFDAKNSDPDLATLLASVRVVPN
                     GEGLEVSLAVSSDQLVSLIKRNTFSAKR"
BASE COUNT          697 a          809 c          973 g          730 t
ORIGIN      
        1 attgaaacca tttcggatgt caattcggct acgcggtgac tgtcaggcca taaaaaaatg
       61 agtaagatgc ttcgtgaggt gagtatgaaa ttcttcggca ggtatgggtt ggcagcaaca
      121 gttttagggg caggggtttt agcggcggtg cttctggcac ccgcgaatcg atcgattatt
      181 tttgcgcaga cgaatgcagg cgcgggtgaa aaaggcgagg tggagcgcgg acgctacctg
      241 gtggaagaag tcgcgaaatg cgcggagtgt cacactcctc gcaatgaacg cggcgaattg
      301 aaggctgatg cctggctacg tggcgcaccg atatggatca agccggtcgc tcctatttcg
      361 aactgggccg atcgcgcgcc ggcgctggcg ggttttccaa gttttacaga ggagcaaggg
      421 gaacggattc tggaaaaggg aactgggccg gaaggcgaag cgttgcggcc gcccatgcac
      481 atctatcaca tgaaccatgc ggacgcgaaa gcgattatcg cgtatctcaa atcattgccg
      541 cgcggtgacc gcactaatta agtccgcagg atttcaggac tccagagttg aagatttatt
      601 gtgcgttcat ttagtgcacg gatttgccgg tttcaacccg cacccatgca agtccatcca
      661 cgcccgcggt aagaacgatg ggttcctgta gttgccaagt gcgcaaatcc agcatcgcga
      721 ttttccctgc actcacacac gaaacgaaag ccatggtgcc ggcaggtgtc accagaattt
      781 cggaagggga gtccggaaga tcgaaagatt tggcgacctt cagcgtggcc atatcgatgg
      841 ccagcagcag atgttttgcc atgcaggcga ccagcagcca ctttccgtcc ggtgtggttg
      901 tagaagcgta agggacagct ggaagatcaa tccagcggga gactttgtag gatgcgacgt
      961 cgtcgccccc ttcggatttt ttgcgatccg ggagatcaat gaccgccagt cgcggcgcgc
     1021 tctgatcgtg agtgaacaag cggcttccgt ccggcgacat ggaaatgcgt tggatggttt
     1081 tcgccacggg gatgacagtg atcaagcggc gcgccgggac gtccagcaca ctgatgtttc
     1141 ctgggcctac gtttgccgta taggcgcgat gtccgtcagg tgaaatcacc agcatgtggg
     1201 attgcgactc tccggtggga atctcgccag tgacctgctg ttttgcagga tcgataattt
     1261 tcaccgtgtt gctcatctcg gcggtcacgt agagcaagcc atccgggccg aattcagcgc
     1321 gatgtggacg caccgggcct agttgaatgg agccagaaac ctggcgtttg gcaatgtcga
     1381 tcacgtcaat cgtggcgccg tcggtgcccg gtttgcccac gccggaattt ccataaattg
     1441 ggacgtaaat gaagcgcgcg tcgggcgaga ccgctgcttc gtggccgctt acgccgaccg
     1501 cgataaccgc gaaggtgcgg cgtatgtcag gctcaaccag caatactttg tgttccgcct
     1561 gattgacaac tacgagcgtg cctttgtcgc tggatccggt ggcatcgacg ccggcgtgca
     1621 aggtgaaaag cgccaatgaa aatacgaccg cgaacagtaa agaaaaactc atatcattcc
     1681 tcgaaaaatt gctgaaatcg tacttgaatt gaacttggat gtgtagcggc gtgcttctct
     1741 catattggcg cctctgtttg aacgattcgc atcctataac attttgcatg cttgtgcggc
     1801 atggcgaacg ttgttaccga tgtgtgttac cgatgtgtgt caaccgatgt gttgaccgcg
     1861 ttcgcagcgg gaagtacgat ggcgcagttt taatcgtgca ttcctcgaaa acgggcggtg
     1921 tgggtaaatg tcaggaactg tttactgttc aaagtgtgga agcctggttg aaggtggagc
     1981 agctttttgt ccggcgtgcg ggcaggcggt gcagccgacc gttgcggcaa gtgcaggctt
     2041 gccattggga gtaagttctg ccgctgcgct tccgtctggc tacgatccca atgcctatgc
     2101 gacggcagct ccagtagcgc cgccggtggt gtacgcgcag ggtagtgtgc agtacgcggg
     2161 gttctggctt cgcgtggttg cgtatctaat cgattcagtt gtcatgggcg tggcgttcat
     2221 ggcccttttc attccgtttg ccatcatgac gggactgaca gcggtgctgg gaaatatcca
     2281 tcccggcgag gaccccaggg atgtaggcgc cgttctcggc ggcacatttt ttctgggact
     2341 attcaccgtt gtgtgcctgg ccttcttggg aggatggctg tatcacgcca agatggaaag
     2401 ttcctcgtgg caggcgaccc tgggcaagaa ggctctgaaa ctgcgcgtaa ccgatcttta
     2461 cggcgcacgc gtaactttcg cgcgcgccag cgggcgccat ttcgccaagt taattaccgg
     2521 gctgattccg ctgggagtgg gattcatgtt ggctggactg acggacagaa ggcaagctct
     2581 ccacgacatg atagccagtt gtgtggtgct gcgagacggg taatcgatca cacgcgagcg
     2641 aattaacgct tggcggaaaa agtgttgcgc ttgattaaac tcaccaactg atcgctgctc
     2701 accgcgaggg atacttccag accttcaccg tttggcacga ctcgcacgga agcgagcagc
     2761 gtggcgagat cgggatccga gtttttggcg tcgaattgcc gcaacaagat tcccgcctgt
     2821 agcagttgag agattgttgc ggcgtcatcg ggcgacgaag aaatcaactg gaaatggacg
     2881 tcgaggtccg cggttccctg gatactgatg accatgcctt gcaatttgtt gaggagtttg
     2941 cccgcttgcg ggaattgcgc ggcttcgggg acgatctgcc gcacagcttg atgagttcct
     3001 ccaggattca aaacacccca aaatattcca cgaccattca cctgtgagat caacggaaac
     3061 atcttttcat tttgaataag actgtcattg gcagccacgc gcacttcgat cagttgctgc
     3121 agcaaccgca gctggccgaa cgccacggtg ctcgaatcga tgaacaccac cgacaaatcg
     3181 tcgcaggaag cgcagggata cagggtgta
//
LOCUS       DATCBB010000944         3558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_160043, whole genome shotgun sequence.
ACCESSION   DATCBB010000944 DATCBB010000000
VERSION     DATCBB010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160043"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2147)
                     /locus_tag="VII37_10345"
     CDS             complement(<1..2147)
                     /locus_tag="VII37_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5070183.1"
                     /translation="MWSFLQDLRFGFRQFRKAPGFVLTAVLSLMLGIGATTAIFSVVY
                     GVLLDPYPYKNNDRMVHIELNDKDGGGPLLFTNGAGFSTIRNVSAVEDVFFQQGSQQN
                     LTGDQIPVSVNVGLYSPNLFEFMGVPPLLGREFSTSDAPGGNPAPVAVLSYLFWQHQF
                     GASRDILNKSMELDHKLYTVIGVVPSRFTWGDSDVYLPAVPTGAQQDYWLSFVRLKPG
                     VKFPAAASEFQMIADEFAKNDKTYPQNRRVAIVTLNEQILGKFAGTLGLLFGAVLTLL
                     VIGCANVSILLLARGTARQHELAVRASVGAGRKRLVRQLLTESVVLSVTGALLGIVAA
                     YKGVTWIVAALPIFSFPHEAAIHVNAPVLIFSASVAILTGILFGISPAWQLSTPQVGA
                     LVQASSSKLTGSARSRNSHRILIAGQLALTLLLIASAGAALKAFQARLHTPLGYDPKN
                     VLSLNVGFPKAANPTWEGRLNAQEAVRKVIADVPGVEDASISTTWFPGLGGFTGKIEI
                     QSRPQLADAQAMFCAVSSHEFSTLHIPLIAGRTFDESETLRAAHFAVVNQAFVKEFLA
                     DTNPIGQGVRSPLLKLERPEILLAQKPDEWFQIIGVVGDARNNGLNRPVKPAVFFPYS
                     FLLPTDVSLLIKTTTNPDTMLNSFKERLRQYNPELVTGRLNRTLMWWLDTQGWGQDRF
                     IATLFALFAVFALALAATGLYSVVSFAVSQRTQ"
     gene            2500..3084
                     /locus_tag="VII37_10350"
     CDS             2500..3084
                     /locus_tag="VII37_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5070184.1"
                     /translation="MCESSDHEVTSLLQAWRAGDEKALDKLAPLVYQELHRTAHRYMA
                     RENSGHTLQTTALVNEVYLRLVNVRKVDWQGRAHFYAICARMMRRVLTDFARSRRYIK
                     RGGDAIHLPFEEALFAGPEDDPDIVDLDEALDVLGAMDQRKTQVVELRFFGGFSVEET
                     AKVLDVSVETVHRDWKLAKVWLMRELSKGKSDGA"
     gene            3074..>3558
                     /locus_tag="VII37_10355"
     CDS             3074..>3558
                     /locus_tag="VII37_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070185.1"
                     /translation="MEPERWRQVEQLYQSVLALEESKRSAFLDDSCSGDEALRREVLS
                     LLAGEERAAKFMETPALEMMAKELAVDQLPRAIAGTDPRQLIGRTISHYRILAKLGHG
                     GMGIIYRAEDTRLGRSVALKFLPEDSRDSTALGRLRREAQAASALNHPHICTIHDVGE
                     F"
BASE COUNT          818 a          919 c         1091 g          730 t
ORIGIN      
        1 tcctgagtgc gctgcgacac cgcgaaggaa acgacgctat agagcccggt ggccgccagg
       61 gctagcgcaa atactgcaaa cagagcgaag agcgtagcga taaagcgatc ctggccccat
      121 ccctgcgtat caagccacca catcaaggta cggttcagcc ggccagtgac caactcggga
      181 ttgtattggc gcaagcgttc cttaaaggag ttcagcatag tgtcgggatt tgtggtggtc
      241 ttgattagca gcgaaacgtc tgtgggcaga agaaaactgt aggggaagaa gactgcgggt
      301 ttgacggggc ggttgagccc gttgttgcgc gcgtcaccga ctacgccgat gatctgaaac
      361 cattcatccg gcttttgcgc cagcaatatt tcggggcgct cgagtttcag cagcgggcta
      421 cgcacgccct gcccgatggg attggtatcc gctagaaatt ctttgacgaa agcttgattg
      481 acgaccgcga agtgcgcggc gcgcaaggtc tccgattcgt caaaggtgcg accagcgatg
      541 agcggtatgt gcagcgtgga aaattcgtgt gagctgaccg cacaaaacat cgcttgcgcg
      601 tcggccaact gaggccggct ctggatttcg attttgccgg tgaaaccgcc taagccggga
      661 aaccatgtgg tagagatgct ggcgtcttcc acgccgggaa cgtccgcgat aacttttcgc
      721 accgcttcct gtgcgttgag gcgtccttcc caagtgggat tcgcggcttt gggaaaccct
      781 acgttgagcg agagaacatt tttcggatcg tagccgagtg gcgtgtgcag ccgcgcttgg
      841 aatgctttga gggcggcccc ggcggaagcg atcagcagca gcgtgagggc cagctgcccg
      901 gcgataagga tgcgatggct attgcggctg cgggcgctgc cggtaagttt ggagctgctg
      961 gcttgaacta gcgcaccgac ctgaggagta gaaagctgcc atgccggtga gatgccaaag
     1021 agaatgcccg taaggatagc gacgctggcg ctgaaaatga gaacgggggc gtttacatgg
     1081 atggcagctt catgcggaaa agaaaagatg ggcagcgcgg cgacgatcca cgtcacgcct
     1141 ttgtaagccg ccacgatacc gagcagtgct cccgtgacgg aaagcaccac ggactctgtg
     1201 aggagctgtc gcaccagtcg tttgcgacct gcgcccaccg aagcgcgcac ggccaactcg
     1261 tgctgccgcg cggtaccgcg tgcgagaagt agaatgctta cgttggcaca accgatgacc
     1321 agcagcgtaa gaaccgcgcc aaacagcaga ccaagtgtcc cggcgaactt gcccaggatt
     1381 tgttcgttga gcgtgacaat agcgacgcga cggttttgcg gataagtctt gtcgtttttc
     1441 gcgaattcat cagcgatcat ctgaaactcc gacgccgcgg ccggaaattt cacgccaggc
     1501 ttgagacgga cgaaggaaag ccaataatcc tgctgcgctc ccgtaggcac cgccggcagg
     1561 tagacgtccg agtcgcccca cgtgaagcgc gacgggacga cgccaatgac ggtgtagagc
     1621 ttgtgatcga gctccatcga tttgttgaga atgtccctgc tggcaccgaa ttggtgctgc
     1681 cagaaaagat agctcagcac ggcgacgggt gcgggattgc cgccgggcgc gtcggaggta
     1741 gaaaattcgc ggccgagaag cggcgggacg cccataaact cgaacaaatt gggcgaatag
     1801 agaccaacat tcaccgagac cgggatttga tcgccggtaa gattttgctg cgagccctgc
     1861 tgaaagaaaa catcttcaac ggccgatacg ttgcgaatgg tggaaaatcc agcgccgttc
     1921 gtgaacagca gcggaccgcc accatcttta tcattgagct caatgtgaac catgcggtcg
     1981 ttattcttgt agggataggg gtcgaggagt acgccgtaga caacgctgaa tatcgctgtg
     2041 gtcgcaccga tgcccagcat tagagagaga accgcggtga gtacgaagcc cggtgctttt
     2101 cggaattggc ggaagccaaa gcgcaggtcc tgaagaaagc tccacataaa gacttaacct
     2161 ccagcttgga tgttctttta gacgaacgag aaaccgaggc ggatagttcc gaaagatttg
     2221 cggtgcggga ggagctttgc tgttttgtgg ctaatgtgaa aggacttgtt atcaactggc
     2281 gctaaaatca cgttgtgtag ctaaagttga tgtttgggat cgtcgcgcag ccgcgaggtc
     2341 atcgccgcaa ccggcaccaa gtacaccgcg caaccagcca gggaagagca aatgaaatga
     2401 attacggagt aattctctct catccaaata catgtcggag gccttgccct tgtgttatcc
     2461 tcccgccaat cttcctgcat ccgttcaacg cgcgattaaa tgtgtgaatc atcggaccac
     2521 gaagtcacgt cgctgctgca agcctggcgt gcaggtgacg agaaagctct cgataaacta
     2581 gctcctctgg tgtaccagga attacatcgt accgcgcacc gttacatggc ccgcgagaat
     2641 agtgggcata cccttcaaac tacggcactt gtcaacgagg tgtacctccg gctagtgaac
     2701 gtccggaagg tcgactggca gggccgtgcg cacttttacg ccatctgcgc tcgaatgatg
     2761 cgcagggttt tgacagattt tgcccgttct cgccgctata taaagagggg aggtgacgcg
     2821 attcatcttc cgtttgaaga agcgctgttt gccgggcccg aagatgatcc ggacattgtg
     2881 gacctggacg aggccctgga tgtccttggc gcaatggacc agcgaaaaac ccaggtggtc
     2941 gaactgcgtt tcttcggagg attcagtgtc gaagaaaccg caaaagttct ggatgtctcg
     3001 gtcgagacgg tgcaccgcga ttggaagttg gcaaaggttt ggctcatgcg tgaactgagc
     3061 aaaggaaaat ctgatggagc ctgaacgctg gcggcaagtc gaacagttgt atcagtcggt
     3121 gctggcgctt gaagagagta agcgctccgc ttttttggac gactcgtgct cgggcgacga
     3181 ggcgctgcgt cgcgaagtgc tttccctgct agctggtgag gaacgggcag ctaagttcat
     3241 ggaaacgccg gccctggaaa tgatggccaa agagttggcc gtggatcaac tgccacgggc
     3301 tattgcaggt acggacccgc ggcagcttat tgggcgcact atttctcatt accgtatcct
     3361 ggccaaactg ggacacggcg gtatgggaat catctatcgc gcggaagata cgcgcctggg
     3421 acgcagcgtc gcgttgaagt ttttgcctga ggattcgcgc gattcgacgg cactgggacg
     3481 cttacgtaga gaggcgcagg ccgcatctgc cctcaaccat ccgcatatct gcactattca
     3541 cgacgtcggt gaatttga
//
LOCUS       DATCBB010000945         1163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_255668, whole genome shotgun sequence.
ACCESSION   DATCBB010000945 DATCBB010000000
VERSION     DATCBB010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255668"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>1163
                     /locus_tag="VII37_10360"
     CDS             40..>1163
                     /locus_tag="VII37_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5070186.1"
                     /translation="MTLGGLALAVGILVDDATVEIENINRNLAMGKETVQAILDGAQQ
                     IAVPAFVSTLCICIVFIPMFFLSGVAKFLFVPLAEAVIFAMLASYMWSRTIVPTMAMY
                     LLSAADEQMPEEGKQGFLRRYQQKFEHAFERMRERYRNALRAALATPILFAGCFLAFC
                     VLSTLLVFVLGRDFFPSVDAGQIRLHFRARTGLRIEETARLADQVDGVIRQTIPQGEV
                     ETVLDNLGVPYSGLNLSYSNAGTFGTSDAEILVQLKPKRQKPTSTYINDLRERLPQDF
                     PGVQFFFQPADIVTQILNFGSPAPIDIQVTGNNQQGNYVLGEKLANEIRHIPGAADVH
                     VQQAFDQPTLHMDIERTRVQNLGLQAKDVAQNLLVSLSSS"
BASE COUNT          252 a          309 c          322 g          280 t
ORIGIN      
        1 gttctgtgcc tcagtgcgct gggcgaaact atcaatatca tgacgctggg agggctagct
       61 ttggcggtcg gcatcctggt agacgacgcc accgttgaaa ttgagaacat caaccgcaac
      121 ttggccatgg gtaaagaaac ggtgcaagcc attttggacg gcgctcagca aatcgccgtg
      181 ccggcgtttg tttccacgtt gtgtatttgc atcgtgttta ttcccatgtt ttttctttca
      241 ggcgtagcaa agtttctgtt cgtgccgcta gcggaagcgg tgattttcgc gatgctggcc
      301 tcttatatgt ggtcgcgcac gatcgtgccc acgatggcga tgtaccttct cagcgcggcg
      361 gacgagcaga tgcccgaaga aggaaaacaa gggtttttgc gccggtacca gcagaaattc
      421 gagcacgcct tcgaacggat gcgtgaacgc tatcgtaatg cgttgcgtgc cgcgctggcc
      481 acgccgatcc tatttgccgg ttgcttcctg gcgttttgcg tgttgtctac tttgttggta
      541 ttcgtgctgg gccgggattt ctttccttcc gtggatgccg ggcaaattcg attgcatttc
      601 cgcgcgcgca ccgggctgcg cattgaggag actgcgcggc tggccgatca ggtcgatggc
      661 gttattcgac aaacaattcc gcaaggagaa gtcgaaacag tgctggataa cctcggcgtg
      721 ccttacagcg gcctgaattt gagttacagc aatgcaggaa cattcggcac atctgatgcg
      781 gaaattctgg tgcagctcaa acccaaacgc cagaagccga cgtcgacata catcaacgat
      841 ctgcgcgaac ggctgccgca ggattttcct ggcgtgcaat ttttctttca acccgctgac
      901 atcgtcacgc aaattttaaa ctttggatcg cccgcgccta tcgacattca agtgacgggc
      961 aacaaccagc aaggcaacta tgtattgggc gagaaacttg ctaacgagat tcgccacatc
     1021 cccggcgccg ctgacgtgca cgtgcagcaa gccttcgatc aaccaacgct gcacatggat
     1081 attgaacgca cgcgtgtgca gaacctggga ctgcaagcca aagacgttgc tcaaaatctg
     1141 ctggtatcgt tgagctccag ctt
//
LOCUS       DATCBB010000946         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_126878, whole genome shotgun sequence.
ACCESSION   DATCBB010000946 DATCBB010000000
VERSION     DATCBB010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126878"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VII37_10365"
     CDS             <1..776
                     /locus_tag="VII37_10365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5070187.1"
                     /translation="PLAKSPLARARYKAAARIVAISKWVAEQLVASGAPKEKISVVYE
                     GVEVPVNPRAQSPHLARMRWGVAGVSDDAKVARESPLLGSVGVLSPDKGHELLIRALA
                     ELRKEYRGCRLLLAGDGPSRPALEALAQELGVSDAVIFAGFVTDIESVYPALDVFLFP
                     SSFEGLGTSLLAAMSYEVPSVAFQCCAFGEIIESEKSGLLVEQGNVAEIVKAVRRLLQ
                     DRHFARAIGAAGRERIKEIFSSDHMAEEMRNVYREVCSR"
     gene            complement(1029..1337)
                     /locus_tag="VII37_10370"
     CDS             complement(1029..1337)
                     /locus_tag="VII37_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5070188.1"
                     /translation="MAPRTRSFTYHRGEQRIPMEIPVFLDGHRRLPGTESTFTENVSA
                     RGARVVSMRRWEQGEKLTFASRTGEFRSSARVAYCQPLQGDGFAVGVEFLDVKGRWVV
                     "
     gene            complement(1343..>2254)
                     /locus_tag="VII37_10375"
     CDS             complement(1343..>2254)
                     /locus_tag="VII37_10375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5070189.1"
                     /translation="TARQPSRETAGALLVVAIAAYVTSAAFLAAPSEPLALHGSALSR
                     PWDLFPGCLFLAAAVCFWQRLRSDKKSQTAPSLYNYSLFLVAALNVICHVSAAFSRQI
                     FDGPFFLAELSKTTSYVVMLGGALLDQARLFDQVRSMAVTDPLTGLANYRRLISVIEA
                     ELDRSRRTHHPFSIVLLDMDGLKTINDRYGHLTGSRALVRLGKILRNHSRAIDTAARY
                     GGDEFALILPEAGRDIATRVFTRIRERLSSEEEAPTLSASAGVACYPEDGDTAEKLLG
                     TADRALYRMKNHGNSNVQNLTRIAACL"
BASE COUNT          504 a          580 c          701 g          469 t
ORIGIN      
        1 atccgttggc gaaaagtccg ctggcccgcg cgcgctacaa agctgcggca cgaatcgtgg
       61 ccatctcaaa atgggttgcc gagcaattgg tggcgtcagg cgcaccgaaa gaaaaaatct
      121 ccgtagtcta tgagggcgtg gaagtaccag ttaatccgcg cgcgcagtcg ccgcatctcg
      181 cgcgcatgcg ctggggcgtc gccggggtct ctgacgatgc aaaagtggcc agagaatcgc
      241 cgctgttagg atctgtggga gtgttgtctc ctgacaaagg gcacgagctg ttgatccgag
      301 ctctcgcgga attgcgcaaa gaatatcgtg gctgccggtt attgcttgcg ggtgacgggc
      361 ccagcaggcc agccctggaa gcactggcgc aggaactcgg cgtgagcgac gcggtgattt
      421 ttgctggatt tgtcacggac atcgagtcgg tgtatccggc cctggatgtg tttttgtttc
      481 cgtcgtcttt tgaagggtta ggaacgtctc tgttggcggc gatgagctat gaagttccgt
      541 cggttgcgtt tcagtgttgc gcgtttggag aaattattga aagcgaaaaa agcggattgt
      601 tggtggaaca gggaaacgtc gcggagatag tgaaggccgt gaggcggctc ctgcaagaca
      661 ggcattttgc acgagcgatc ggggcggctg gccgcgagcg catcaaagag atattttcca
      721 gcgatcacat ggcggaagag atgaggaacg tttatcgtga agtgtgctcg cgctgactcg
      781 ttgagaacgt gcaagaaaaa cgtctgattg gggctgccca aataaccaac tagatccagg
      841 ctattgcagc agtaactcga cactctaaat cagctaataa acactgcatg aaaagcaaaa
      901 agcgccggac ggttggtgct cgccgcgacg cttgaagctc agtaaaaatt tgcgctagtt
      961 tactgttttt ccctttttcc agcggtcccc tcctatccgc ctgccgaata cgtcaagcga
     1021 ttttagaatt aaacaaccca tcgtcctttg acatcgagga actccacccc aactgcaaag
     1081 ccgtcgcctt gcagtggctg gcagtaagcc actcgagccg aggaacgaaa ctctccggtg
     1141 cgcgatgcga acgttaactt ctcgccttgt tcccagcggc gcatactgac cacgcgcgcg
     1201 ccgcgcgcac tcacattttc tgtaaaagtc gattcggtgc cgggcagcct gcggtgtcca
     1261 tccaggaaca ccgggatttc catgggaatg cgctgctctc cgcggtgata ggtgaacgat
     1321 cgtgtgcgtg gggccatttt gttcacaggc acgccgcaat gcgcgtgaga ttttgtacgt
     1381 tgctgttgcc gtgatttttc atgcgataca gagcgcggtc tgccgtgccc aacaatttct
     1441 cggcggtgtc gccgtcttcg ggatagcaag ccacgccagc gctggcggaa agcgttgggg
     1501 cttcttcttc ggaagaaagg cgttcgcgaa tgcgggtgaa tacgcgggtg gcaatgtcgc
     1561 ggccagcctc gggcaagatc aaagcgaact cgtctccgcc ataacgcgcg gcggtatcga
     1621 tggcgcgtga atgattgcgc agaatttttc cgaggcgaac cagagcgcgg ctaccagtca
     1681 aatgtccgta gcggtcattg atagttttca gcccgtccat atcgagcagc acgatgctga
     1741 aaggatggtg agtgcggcgt gaacgatcga gttccgcttc gatgacgctg atgagccggc
     1801 gatagttagc gagcccggtg agcgggtcgg ttacggccat ggaacgcact tgatcaaaca
     1861 agcgcgcctg atccagcaga gcgcctccca acatcaccac gtagctggtg gtcttgctca
     1921 gttcagcgag gaagaacggt ccatcgaaaa tctggcgaga gaaggccgcc gaaacgtggc
     1981 agatgacatt aagcgcagcc acgaggaaca acgaatagtt gtacagggag ggagcggttt
     2041 gagatttctt gtcgctccgg aggcgttgcc agaagcacac ggcagcggca aggaataaac
     2101 atccagggaa aagatcccaa ggacgagata gtgcgctccc gtgcagcgca aggggctccg
     2161 aaggagcagc caggaatgct gcactggtaa cgtaagcggc gattgcgacc acgagcaagg
     2221 cgcccgccgt ttcgcggctt ggctggcggg ccgt
//
LOCUS       DATCBB010000947         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_62068, whole genome shotgun sequence.
ACCESSION   DATCBB010000947 DATCBB010000000
VERSION     DATCBB010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62068"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /gene="purH"
                     /locus_tag="VII37_10380"
     CDS             <1..283
                     /gene="purH"
                     /locus_tag="VII37_10380"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY5070190.1"
                     /translation="FAKNGATLGVGAGQMSRVDSVKLAVMKAQSSLQGSVVASDAFFP
                     FPDGVEEAAKAGATAVIQPGGSVKDADVIAAADRLGMAMVFTGMRHFLH"
     gene            504..2669
                     /locus_tag="VII37_10385"
     CDS             504..2669
                     /locus_tag="VII37_10385"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5070191.1"
                     /translation="MGYFLFGAFMNSQRMILAFLLASAMGGTSLCQTGGPPASSTEKP
                     QDKTNVPAASHDQQTSESSAQVTKTPSRADAYYNYTMGHIFEQQYESTSKPEYATQAI
                     EAYKKAYALDPKSPVIGERLAEMYWKAQRTHEAVTEAQALLKRDPNDIQSRRLLGRIY
                     LRSLGDFSVGNGQSEIVERAIEQYAEIYRLDPGDAESALWLARLYRLRNEHDKAEAVL
                     RGMLKEDPENEPAIEQLTQLLLDEGKAPEAIALLEEVTRRSPSPAMLDLLGDAYTQSH
                     DLPKAEAAYRKATELDPSELSHQRGLGQTLMSEEKYSDALAVYQKLTDLMPDDAENYL
                     RIAQIYRELHDLGKAEDSLVKARQFAPGSLEVMYNEAMLYQAQGRNEDAIRVLSDAVT
                     GIKSQPQGAPSRSHSLGILYQQLGQLYRDTQNYQAAIYTYQELGRLGEEEDRRARLLM
                     MDVYRAAKDLPKALQTGKDAVAKYPSDMSLRSSEALLLGESGQTDDGVKMLRTQLTGK
                     PGDRDVYLNIAQVYERGRRYKEAEEAAKAAEVLPGQPRDNEMVWFLLGAIYERQKFFD
                     RAEEQFKKVLAVNPRNAPVLNYYGYMLGDLGIRLDEAEDLVRRALKEEPFSGAYLDSL
                     GWIYLKENKLGESEVALRKALEHESHDATIHSHLGDLYAKTGRPEMAASEWEKSLAEW
                     KHSLPADVENDKIAELEKKLSQMKHRVAQKSSAQETKPQ"
BASE COUNT          639 a          694 c          884 g          542 t
ORIGIN      
        1 atttgcgaaa aatggcgcga cgctcggcgt tggtgcggga cagatgagcc gtgtggattc
       61 cgtgaaattg gcggtgatga aggcgcaaag ttcgttgcaa ggcagcgttg tggcctcgga
      121 tgcattcttt ccttttcccg atggcgtgga ggaagcagcc aaggctgggg ccacggcggt
      181 tattcagccg ggtggctcgg tgaaggacgc ggacgtgatt gcggcggcgg accgcttggg
      241 aatggcgatg gtctttacgg ggatgcggca cttcttgcac tgaaacgagg cggcgggaag
      301 cttacttgtt cattcctaag ccgcgaaagc atctaccact gcatagtcga cgttcctaca
      361 tcgtctggta tgcccggaac ttcgcaaagt cagcacatgg ggcttgcagg gatggctcgg
      421 ttcaccgctc agactcccag tgatagcgcg tgcagcgcct ggaaacggag ccgggattga
      481 cttttgaccg cgctcacagg cgcatgggat actttctttt tggggcattc atgaactctc
      541 aacgaatgat tctcgcgttt ttgctggcca gtgccatggg cggcacaagc ttgtgtcaaa
      601 ccggaggccc gccggcatca tcgacggaaa agcctcaaga caagaccaac gtgccggccg
      661 catcgcacga ccagcagacc agcgagtctt ccgcgcaggt cactaagacg ccctcgcgcg
      721 ccgacgccta ttacaactac acgatgggcc acatcttcga gcagcagtac gagtccacca
      781 gcaaaccgga atacgccacg caggcgatcg aagcttacaa aaaggcgtac gcgcttgatc
      841 ccaagtctcc cgtgattggc gagcggctgg ccgaaatgta ttggaaggcg cagcgcaccc
      901 acgaagcggt tacggaagcg caggcgttgc tgaagcgcga tcccaacgat atccagtcgc
      961 ggcgcctgct gggcaggatt tatctgcgca gcctgggcga tttcagcgtg ggcaatgggc
     1021 aatctgagat tgtggaacgc gccatcgagc agtatgcgga aatttaccgg cttgatccgg
     1081 gcgatgcgga atcggcgctg tggcttgcgc gactttaccg cttgcgaaac gagcacgaca
     1141 aggccgaagc ggttttgcgc ggcatgttga aagaagatcc tgagaacgag ccggccattg
     1201 agcagttgac gcaattgttg ctggatgaag gcaaggcccc cgaagcgatt gcgctgcttg
     1261 aagaagttac gcggcgatcg ccatcaccgg cgatgctcga tttgctcggc gatgcgtaca
     1321 cgcaatcgca cgacttaccg aaagccgaag ccgcttatcg caaagccact gaactggatc
     1381 cttccgagct gagccaccaa cgcgggcttg gccagacatt gatgtcggaa gaaaaatatt
     1441 ccgacgcgct ggcggtttac cagaaattga cggatttgat gccggatgat gcggaaaact
     1501 acctgcgcat cgcgcagatt taccgcgagc tccatgattt gggcaaggcg gaagatagtc
     1561 tggtgaaggc gcggcagttc gcgccaggca gtttggaagt gatgtacaac gaggccatgt
     1621 tgtatcaggc gcaggggcgc aacgaggatg ccattcgcgt attgtctgac gcggtcacgg
     1681 gcatcaaatc ccagccgcag ggagcgcctt cgcggtcgca ttcgctgggg attttgtacc
     1741 agcaattggg gcaactgtat cgcgacacgc agaattatca ggcggctatt tatacctacc
     1801 aggaacttgg gcggctcggt gaagaagaag accggcgggc gcgtttgttg atgatggatg
     1861 tatatcgcgc ggccaaggat ttgccgaaag cgctgcaaac gggcaaggat gcggtggcca
     1921 aatatccgag cgatatgtcg ctgcgttcga gcgaagcgct gttgctgggc gagagcgggc
     1981 aaaccgacga cggcgtgaag atgctgcgca cgcagctcac cgggaagccg ggcgaccgcg
     2041 acgtttattt gaatatcgcg caggtgtatg agcgggggcg gcgttataag gaagcggaag
     2101 aagccgcaaa ggccgcggaa gttttgcccg gtcagccgcg tgataacgaa atggtgtggt
     2161 ttttgctggg cgcgatttat gaacggcaaa agttttttga tcgcgcggaa gagcaattca
     2221 agaaagtctt ggcggtaaat ccgcggaatg cgccggtact gaattattac gggtacatgc
     2281 tgggcgatct ggggattcgg ctggatgaag ccgaagattt ggtgcggcgg gcgttgaagg
     2341 aagagccgtt tagtggcgct tatctggaca gcctcggctg gatttatttg aaagaaaata
     2401 agttgggcga atcagaagta gcgctgcgca aagcgctgga gcatgagagc cacgacgcga
     2461 ctatccattc gcatctcggc gatctgtatg ccaagaccgg gcgcccggag atggcggcgt
     2521 cggagtggga aaagtcgctg gcggaatgga agcattccct gcccgcggat gtggaaaacg
     2581 ataaaatcgc ggagctggaa aagaagctct cgcaaatgaa gcatcgcgtc gcgcaaaaat
     2641 cctctgcgca agaaaccaag ccacaataag gcgcatgcgc gaagtccgca ttccgtcctt
     2701 tgcaaaaatc aatctacgct tggacattct gggaaaacgt gcggacggtt atcacgagc
//
LOCUS       DATCBB010000948         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_190975, whole genome shotgun sequence.
ACCESSION   DATCBB010000948 DATCBB010000000
VERSION     DATCBB010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190975"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VII37_10390"
     CDS             complement(<1..893)
                     /locus_tag="VII37_10390"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY5070192.1"
                     /translation="METMGSDECKASQRSALRFFRISMLCFLTATAQVSTVHGANVQR
                     ARHARNAARVMVGLDVLESERFAPLRGKHIGVITNHTGLDAQGRTTIDALTHAPGIQV
                     VALFTPEHGLGGTKDENISSTKDPVSGLPVYSLYGETRRPTDEMLAGIDALVFDVQDA
                     GVRFYTYTTTMAYCIEEAAKHKITFFVLDRPNPLGGEIIEGPMLDADKTSFTAYFRLP
                     IRYGLTIGELAQLFNEENHIQCDLHVVAMKNWRRNYFYEETGLRWLPPSPNLRTLKGS
                     VLYPGLEILQNGGVSVGRGTEA"
     gene            1055..>2195
                     /locus_tag="VII37_10395"
     CDS             1055..>2195
                     /locus_tag="VII37_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY5070193.1"
                     /translation="MKGDNGRVKNPCARLLPLFLIAIIFSMAYTARSQNNSSPAKKSV
                     VPKPVTRARTLSSANKRWVEATLRKMTDDEKVGQLLFTTYHGSFTSTDADAYRKMMHD
                     VDDLHVGGFINVTQMSPLGIIKSQAYPTAVLTNQLQARSKLPLLIGADFERGSAMRLD
                     EGTSFPTAMALAAGGNPADAYTMGKITALEARAVGIHWIYAPVSDVNNNPGNPIINTR
                     SFGEDPQRVAEYVAAFVHGVQENGGLATAKHFPGHGDTAADSHIDLPVIRADKDRLEN
                     LELVPFRSAISAGVGSIMTGHLSIPALEPDPNTPATLSNNVLTGLLRDELHFEGLVIT
                     DAMDMGGITTRYAPGEAAVRAFLAGADALLMSPVPDAAFEALQAAV"
BASE COUNT          474 a          632 c          595 g          494 t
ORIGIN      
        1 gcctccgttc cgcgtcccac agaaactccg ccattttgta aaatctccag tcccggatag
       61 aggaccgagc ctttcagcgt gcgcagattt ggcgaagggg gtagccagcg cagaccggtc
      121 tcttcataaa aataattgcg ccgccaattt ttcatcgcga ccacgtgtaa atcacattgg
      181 atgtgatttt cttcgttgaa cagttgagcc agttcaccga tcgtgagccc gtaacgaatc
      241 ggcagccgaa aataggctgt gaagctagtc ttatccgcgt ccagcatggg tccttcgatg
      301 atttctccgc caagcgggtt gggccgatcc aatacgaaga atgtaatttt atgcttggcc
      361 gcttcttcaa tgcagtacgc catcgtggtg gtgtaggtgt agaagcgcac gccagcgtct
      421 tgcacatcaa aaaccagggc gtcgatgccg gcaagcattt cgtcggtagg gcgacgggtc
      481 tcgccgtaca aactgtagac cggcaacccg ctcactggat ctttcgtgga ggaaatattt
      541 tcgtccttgg tcccgcccaa gccatgttct ggagtgaaca gcgccaccac ctgaattccc
      601 ggcgcgtgag taagcgcatc tattgttgtg cggccctgcg cgtcgaggcc ggtgtgatta
      661 gtaatcacgc cgatgtgctt gccgcgtagt ggggcgaagc gctccgactc gagcacgtcc
      721 aggccgacca tcacccttgc ggcattgcgg gcatgccgcg ctcgctgtac attcgcgccg
      781 tgaacagtgc tgacctgcgc tgtcgccgta aggaagcaaa gcatcgagat tcgaaaaaac
      841 cgcagcgctg atcgctgtga cgctttacac tcatcggagc ccatggtttc catcgaatgc
      901 ctcataagtc gcaataatat tctggaacgt ctctcacgcg tgttgtttgt ggcctccaag
      961 tgcctatgct atatacaaag tcagagcatg cacggcacca aacgggtcgt tttagaccaa
     1021 gggtttcgat cgaggatttc gggcaagggt ttcgatgaag ggcgataatg gccgggtgaa
     1081 aaatccctgc gcccgtctgc tcccgctgtt ccttattgcg atcatttttt ctatggccta
     1141 taccgcgcga agtcaaaaca attcctctcc cgcaaagaaa tccgttgttc ccaaaccggt
     1201 gacgcgagcc cgtacgcttt cttccgcaaa taaacgatgg gtggaagcca cgctgcgcaa
     1261 aatgactgac gacgaaaaag tcggccagtt gctattcacc acctatcacg gcagcttcac
     1321 atctacggat gcggatgcct atcgcaagat gatgcacgac gtggatgatc tgcacgtcgg
     1381 cggatttatc aacgtcacgc agatgtcgcc gctgggcatt attaaaagcc aggcttaccc
     1441 cacggcagtg ctaaccaatc agcttcaggc acgctccaaa ttgcctttgc tcattggcgc
     1501 cgacttcgaa cgcggctcgg cgatgcgcct cgacgaaggc acatctttcc cgacggcgat
     1561 ggctttggct gcgggtggga atcctgccga cgcttacacc atgggcaaaa ttaccgcgct
     1621 cgaagcccgc gccgttggca ttcattggat ttatgcgccg gtttcggatg taaataacaa
     1681 tccgggcaat cccatcatca atacgcgatc cttcggcgaa gacccgcaac gcgtcgcgga
     1741 gtacgttgcc gctttcgtgc acggggtgca ggagaacgga ggccttgcga ccgccaagca
     1801 tttcccgggg cacggcgata ctgcagccga ctcacacatc gatcttccgg tgattcgcgc
     1861 cgacaaggat cggttagaaa atttggaact ggttcccttt cggtccgcga tttccgccgg
     1921 cgtgggaagc atcatgaccg gccatctttc cattcccgcg ttggaacccg atcccaacac
     1981 tcccgcaacg ctgtctaaca atgtcttaac cgggcttctg cgcgatgaac tgcattttga
     2041 aggcctagtg ataaccgatg ccatggatat gggcggcatc accacacgtt atgctcccgg
     2101 cgaagcggcc gtgcgtgcct ttctcgcggg agccgatgcg ctgttaatgt cgccggtgcc
     2161 cgacgcggca ttcgaagcgt tgcaggccgc agtga
//
LOCUS       DATCBB010000949         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_94446, whole genome shotgun sequence.
ACCESSION   DATCBB010000949 DATCBB010000000
VERSION     DATCBB010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94446"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..482
                     /locus_tag="VII37_10400"
     CDS             18..482
                     /locus_tag="VII37_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5070194.1"
                     /translation="MTTGIIDKKRLIFPRLAGLYETLSPASYSFMRFATGVVLLPHGI
                     QKIMLGSAVNLATNIDKQTGLPSPLMWAYFAIFAESVCAICLTIGLFTRVAALIILIE
                     MVTIVFYFQWQFGYFWTVKGYEYALLWALLCLAILFRGGGRYSVDRLLGKEF"
     gene            complement(912..1796)
                     /locus_tag="VII37_10405"
     CDS             complement(912..1796)
                     /locus_tag="VII37_10405"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5070195.1"
                     /translation="MNVTFIGLGNMGSNIAQCILKAGFDLTVWNRTVSKMQPLVEQGA
                     KQAVSAKEATSGADVVVTSLMDDQSILDNLQAENGILAGLRPGAVHVCVTTISPRCAD
                     ELEQIHKKHGSFYVAAPVSGRPEAAASGQLISYLAGASAAVEEVRPVCKAYSKQIIVI
                     SERPRMANVMKLAINYNVISAIEVISETYVFAEKCGLPLEHLRDFYQQLWFAHPAAQL
                     YAEKLRKRDFAGRGGFVMAGGLKDVRLMLSTAAEVGTKLDVGELVERNLSMGVDAGMG
                     EQDWSSIHELARKKAGLD"
     gene            complement(2413..2628)
                     /locus_tag="VII37_10410"
     CDS             complement(2413..2628)
                     /locus_tag="VII37_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070196.1"
                     /translation="MIAAPKRKNMAGKRRVSLVIAGSLAGTTTDLRTGRIVLALQARS
                     GKTFDIAKKNHPRKISHVADAVEKPGI"
     gene            2651..2941
                     /locus_tag="VII37_10415"
     CDS             2651..2941
                     /locus_tag="VII37_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY5070197.1"
                     /translation="MAESGIASIYAYNGEKTANGERANPSRLTAAHRTLQFGTHVRVT
                     NQRNGRSVTVRINDRGPFVRGRVIDLTPAAAQALGFSGIEGLARVKLEIAHM"
BASE COUNT          658 a          902 c          858 g          756 t
ORIGIN      
        1 cgtgagaaga gggaaacatg accacgggca tcatcgacaa aaaacggttg atcttccctc
       61 gcctcgcggg cctttacgag accttatcac cagcgagcta ttccttcatg cgcttcgcca
      121 ccggcgtagt cctgctgccg cacggcatcc agaagatcat gcttggcagc gccgtgaatc
      181 tggccaccaa tatcgacaag caaactggct tacccagtcc tctaatgtgg gcctattttg
      241 cgatcttcgc tgaatctgtg tgcgcgatat gtctcacgat tgggcttttt acccgcgttg
      301 cggcgttgat catcttgatc gagatggtga ccatcgtttt ttatttccaa tggcaatttg
      361 gctacttctg gaccgtcaag ggttatgaat acgccttgct gtgggcgctg ctctgtctcg
      421 ccatcttatt ccgtggcgga ggccgatatt cggtcgatcg actgctcggc aaggagttct
      481 aatcgcgttc ctgtgggccg cctcagttgg cggcctcttt catttcaaac caagggcgtg
      541 atgtctgctt gtggcacatg actgcgttcg tggcaccgca ccatttagga cgcgattgga
      601 caacaacgga caaacgtcaa ttttggcccg cgacggctat gacgtgaatg acccatgagc
      661 gacaattcac gtaattccgc cacgtcaaat gacgcattgc gaaaggttta ttcgatcacc
      721 gcatcggcga tggagaggag ctttgtgcac gcgccaatat gacgccgtcg ggtcggcgct
      781 tcagcatgta gtagtcctta aacgtctctt tgtactcttc gaattgtggc gtcggcatga
      841 acggttgaag tgacatgcgt aggttcatgt ctatgagatg acggggaatt ttaaaaagac
      901 gcgatcaaca attagtcaag tccggctttc tttcgcgcaa gttcgtggat cgaactccaa
      961 tcctgttcgc ccatgccggc atcgactccc atcgagagat tgcgctcaac gagctcgccc
     1021 acgtccagct ttgtcccgac ctccgcagcc gtagacagca tgaggcgcac atctttgagg
     1081 ccgcccgcca tgacgaagcc gccgcggccc gcgaagtcgc gctttcttag cttttccgcg
     1141 tacaattgcg ccgcagggtg cgcaaaccaa agctgctgat agaagtcgcg taggtgctcc
     1201 agaggcaatc cgcatttttc ggcaaagacg tacgtttcgc tgatcacctc gatcgcagaa
     1261 atgacgttgt agtttatggc aagcttcatg acgttcgcca tgcgcgggcg ctccgagatc
     1321 acaattattt gcttggaata agccttgcag accggcctca cttcttcgac ggcggcagaa
     1381 gcgccagcga gatacgaaat gagctgccca ctcgccgccg cttctggacg cccacttact
     1441 ggagctgcga cgtagaagct accgtgcttt ttatgaatct gctctaattc atccgcgcag
     1501 cgtggcgaga tcgtcgtcac acagacatgg acagcgccgg gacgcaatcc ggcaaggatt
     1561 ccattttccg cctggaggtt atcgagaatg gactggtcgt ccataaggct ggtgacaacc
     1621 acatccgcgc cgctcgtcgc ctccttcgcg cttaccgcct gctttgcccc ctgctcgacg
     1681 agcggttgca tcttcgaaac cgtgcgattc cacacggtca agtcaaagcc cgccttcaat
     1741 atgcattggg cgatgttcga gcccatgtta cccaagccaa tgaatgtcac attcatgatg
     1801 gtcccccgtc gcaacggatc atgcgacatt ttattgcgca atgcgaggca ccaagacaca
     1861 gcaaaagccc agatcatcta accgggcgtt agcttgcggt gcgcgtcact gctctcgtgt
     1921 cggcttctga tgctgtggac ggctcctcca tcggcacgtg agtgccatga aagtggctgc
     1981 tgttctgctg caagtgcgat gtcgccttct ggcacctagc ggacaatcca accgcagccg
     2041 cgattgtccg cttctggacc aaagcggaca aaaggtagtt ttcggccatc ggcggtttgt
     2101 ccgcttatga cccattaagc gacattgggg gcgcgcagct gaaagcacta tgggccggcc
     2161 gccgccaccg gctgttaggt tcacgcagga atacgctgcg gggcaggcct tttatggtta
     2221 acttggcgtt aatccgaatc cttcacagct aataccgtca gagttggctt aaactatcgc
     2281 ttctgatcgt caatcgaacc cgcaagggag aaacatcatg aatacgaatc tgcgcgcccc
     2341 aaatgtttgg ctcttcacgg tcgcagcggt tctggcgctc attgggatag cgcaacatct
     2401 gtcaatcgtg ccttatatcc cgggtttttc aaccgcatcc gctacatggc ttattttcct
     2461 tggatggttt tttttggcga tatcaaatgt tttaccggaa cgcgcctgaa gggccaaaac
     2521 gatacgacct gttctcaaat cggtggttgt gccagccaag ctgccggcga tgactaaact
     2581 cacgcgtcgt ttgcctgcca tgttcttgcg cttgggcgcc gcgatcatgt ttatttgttc
     2641 atccgctgcg atggctgagt ctgggattgc gtccatctac gcatacaatg gcgaaaagac
     2701 tgcaaacggg gagcgcgcaa atcccagccg gcttaccgct gcgcatcgaa cgttgcagtt
     2761 cggcacgcat gtgcgcgtta ccaatcagcg caacgggcgc agcgtcaccg tgaggatcaa
     2821 cgaccgcgga ccgttcgtgc gtggccgcgt gatcgacctt acccccgcgg cagcgcaggc
     2881 cttgggtttt tccggaatcg aaggactcgc gcgcgtgaag ttagaaattg cacacatgta
     2941 gtctggcccg ctgccgcatc ggatgggaga atgtctttaa ggggtaaggc ggagtgcgcc
     3001 gggagaccgg cgtatcgact gcgctcaagt aacacgccat aagtcagagc tagagcaatg
     3061 aggccgcccc gtttgcggcc tctttcattt caaaccggac gtgcgatgtc gccttttggc
     3121 acttcgcgtc attcgccgcg atgcaccatt tccggtcgct attgggtgta cagc
//
LOCUS       DATCBB010000950         1365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_288691, whole genome shotgun sequence.
ACCESSION   DATCBB010000950 DATCBB010000000
VERSION     DATCBB010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288691"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..>1365)
                     /locus_tag="VII37_10420"
     CDS             complement(32..>1365)
                     /locus_tag="VII37_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY5070198.1"
                     /translation="PKGEWWTVFHDDELSGLEKQAIDANQTIKVSIARLEQARATAAV
                     QISTLFPTLGLTPGATTPTAERQGLSGDRPSSGIPIKGAVQQNSFSLPFVAGYELDLF
                     GRRRRSFESAEATYQASAADLENVRLVVTAELAGDYFSLRELDSEIGILNRTVQALQK
                     GLDLVNSRHNGGVASGLDVAQEETLLRTTQTQAQLRQQQRKNFEDAIAVLVGRPAPDF
                     HLASNELNAAAPPPFNAGLPSDLLERRPDIAEAERQMASANAQIGVAKAAYYPTIDLF
                     ATAGPQVSDLAKLANLSSMFWAVGANVSESIFTGGARRAQVQFAQAGFDANVANYRGT
                     VLNAFREVQDDLTALNVFIQAQQTQQQAVDAAQRTLDISTNRYTGGLVSYLDVVTAQQ
                     SLLNSQQELVTIQGQRLTTSVLLVKALGGGWDASSLAAIQVKAKLRDIVAP"
BASE COUNT          284 a          396 c          371 g          314 t
ORIGIN      
        1 cttgaaaatg catgaagcga aataaaaatt gttacggcgc gacgatgtcc cgtaactttg
       61 cttttacctg tatcgctgcc aggctcgatg catcccaacc tccgcccagt gctttgacca
      121 gtagcacgct ggtggtcagc cgttggccct gaatcgtcac cagttcctgc tggctgttga
      181 gcagactctg ctgtgcagtt acaacatcca gataacttac caagccgccg gtgtatcgat
      241 tggtggaaat gtccaatgtc cgctgggcgg cgtcaaccgc ctgctgctgc gtttgctgtg
      301 cctgaataaa tacgtttaaa gctgtcaaat cgtcctgcac ttcgcgaaat gcattcaaca
      361 ccgtaccccg gtaattcgcg acattggcat caaagccggc ttgcgcgaat tgcacttggg
      421 cgcgacgcgc tccaccggta aaaatacttt ccgaaacatt agctcccacg gcccaaaaca
      481 tactcgataa atttgcgagc ttcgcgaggt ccgacacttg cgggcccgcg gtcgcaaata
      541 aatcgatggt cggataatag gcagccttgg ctacgccaat ctgtgcgttc gcggaggcca
      601 tttggcgctc ggcttccgcg atgtcgggcc gccgttccag caaatcggat ggcaagcccg
      661 cattgaaggg cggaggagcc gcggcattca actcgttgga agccaaatga aaatccggcg
      721 cgggtcttcc gaccaaaacc gcgatggcat cctcaaaatt ctttctttgt tgctgccgca
      781 gctgcgcttg ggtttgtgtt gtgcgcaaca gcgtctcttc ttgcgcaacg tccaggccag
      841 aggcgacgcc gccgttgtgt cgcgaattta cgaggtccag ccctttttgc agcgcctgca
      901 cggtgcgatt caaaattcca atttcgctgt cgagctcgcg taacgaaaaa taatctcccg
      961 ccagctcagc ggtgacgacc agccgcacat tttccaaatc tgctgcgctg gcctggtatg
     1021 tggcttccgc cgattcaaag ctgcgacgcc gtcgtccaaa cagatcgagc tcgtacccgg
     1081 ctacgaatgg aagcgaaaac gaattctgct gcaccgctcc tttgatcggt atgccagagc
     1141 tggggcggtc gcccgaaagc ccttggcgtt cagcggtggg agtggtggcg cccggtgtca
     1201 acccaagggt cggaaagagt gtggagattt ggaccgctgc cgtggctcgc gcttgctcca
     1261 atcgcgcgat ggacactttg atggtctggt tggcatcgat ggcttgcttc tccaagccgc
     1321 tcagctcatc gtcatgaaaa accgtccacc actcgccttt tggta
//
LOCUS       DATCBB010000951         1096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_485563, whole genome shotgun sequence.
ACCESSION   DATCBB010000951 DATCBB010000000
VERSION     DATCBB010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485563"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VII37_10425"
     CDS             <1..902
                     /locus_tag="VII37_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070199.1"
                     /translation="RDHEVCARCHTKDCIRGRRDRPSGPDFVVLQRGCELALFQELKV
                     GNMDCTFCLDCVHACPHDNVGILSRVPAAELMTDPMRSGIGLFSRRSDIAALAMVFCF
                     GALANAFGMVSPAYAVEKWLGGVLRVQAQAPILGVIFVLFLIVEPVILLSLAAWLTKI
                     FNGSSQALLPLVVRYSYALAPLGFGMWLAHYGFHFFSGLYAFIPVTQNALATLGWPIL
                     GTPAWRLTGIPVRFIQPMDLGFLLLGLAGSLLVVFRLAEEDSPGRVMRAFTPWASLSV
                     IIWLASVWLMYQPMEMRAMLMGG"
BASE COUNT          185 a          260 c          335 g          316 t
ORIGIN      
        1 tacgcgacca tgaggtttgc gcacgctgtc ataccaaaga ttgcattcgt ggacgtcgcg
       61 accggccgtc tgggcctgat tttgtggtgc tacagcgcgg ttgtgagctg gccctttttc
      121 aagaactcaa agttggcaac atggactgca ccttttgcct cgattgtgta cacgcctgtc
      181 ctcacgacaa cgtaggaata ctcagccgtg ttccggcggc ggaattgatg accgatccga
      241 tgcgttctgg aattggtttg ttttcgcgca ggagcgatat agcggcgctg gcgatggtgt
      301 tttgctttgg ggctttggcg aacgcgttcg gcatggtcag cccggcctat gcagtcgaaa
      361 aatggttggg cggagtcctg cgcgtgcagg cgcaggcgcc cattctcggc gtgatctttg
      421 tattgttttt gattgttgag cctgtcattt tgctatcgct tgccgcctgg ctcactaaaa
      481 tttttaatgg tagttcacag gcgctgcttc cgttggtggt gaggtattcg tacgccctgg
      541 cgccactcgg ttttggtatg tggctcgcgc attatgggtt tcattttttt agcgggcttt
      601 atgctttcat tcctgtgacg caaaacgctc tcgctaccct gggctggccg atcctgggca
      661 ctccagcatg gcggttgacc ggaattccag tgcgatttat tcagcccatg gatctcggat
      721 tcttgttgct cggcttggcg ggatcactcc tggtggtatt tcgattggcc gaagaagatt
      781 cgccgggaag agtgatgcgc gcgttcacgc catgggcatc gctcagcgtg attatatggc
      841 tcgcttctgt gtggctaatg tatcagccga tggaaatgcg cgcgatgttg atgggcggct
      901 aggtaaaaaa gagattcgct atggctagtt tatcgacagg ggcgcaaaga gcggggtggt
      961 tagcggggtt gcgcgagcat ttgaagattt caatgccggt gcttgccgcc tttattttgc
     1021 tgtgcctttg cgtttcatgc gcgccattgg cgcgtgggca taacggtcct ccatttccga
     1081 ttattgtgga tcagcg
//
LOCUS       DATCBB010000952         1427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_450081, whole genome shotgun sequence.
ACCESSION   DATCBB010000952 DATCBB010000000
VERSION     DATCBB010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450081"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..>1427
                     /locus_tag="VII37_10430"
     CDS             46..>1427
                     /locus_tag="VII37_10430"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY5070200.1"
                     /translation="MQLVIHAPFGGRINKAWGLALRKRFCRSFNFELQAAATDNGLNI
                     ALAEQHSFPLADVFHFLNAETVQEVLIQAACTSPIFATRWRWDANRSLALLRFQGGKK
                     VPPQIQRMRADDLLASVFPDVAACQENIVGDIQIPDHPLVNEVMKDVLHEAMDIDGLK
                     HLLAGMADGRIRCVAVDTPVPSQFSHEILNANPYAYLDDAPLEERRARAVEMRRILPE
                     SVLEEVGKLDPAAIAQVRQEAWPDVRDADELHDVLHTLIALPISAEGSNTDRATLGWT
                     APSATLRAGSCGCPYVAGAHSQWQIYFRYLLDQGRVTTAQAADRSYWVTAERANAFAL
                     LFPDATFIPQLAEIDGTLPIREDAMLALVTGWMSHIGPVTAKELASLLGLSSSEVDQA
                     LLRMEASGAILRGKFTDKASRAGAPFDFAQGRSAPHKQETEWCDRRLLARIHRLTVAT
                     LRKQIEPVTAA"
BASE COUNT          294 a          410 c          424 g          299 t
ORIGIN      
        1 caagacaccg tgattgcgga acgattcttc gatgagagcg gcggaatgca gcttgtgatc
       61 cacgcgccct tcggcggccg catcaacaag gcctggggac tggctctgcg caagcgtttc
      121 tgccgaagct ttaatttcga attacaagcc gctgccaccg ataacggact caacatcgcg
      181 ctggccgagc agcacagttt tccattggcc gacgtttttc attttctgaa cgccgagacg
      241 gtgcaggaag ttttaatcca agccgcgtgc acttctccga tatttgccac gcgctggcga
      301 tgggacgcca accgctcgct ggccctgctc cgcttccagg gtggaaagaa agttccgcca
      361 caaattcagc gcatgcgtgc ggacgatctg ctggcctcgg tttttcctga cgtggccgcc
      421 tgccaggaaa atattgtggg agatatacag atccccgatc atccactcgt caacgaagtc
      481 atgaaagacg ttcttcatga ggcgatggac atcgatgggc tgaagcattt gttagcagga
      541 atggcggatg ggcgaatccg ctgcgtggcc gtggatacgc cagtgccatc gcagttctcg
      601 catgaaattc tgaatgcgaa tccttacgca tacctagacg acgctccgct tgaggagagg
      661 cgggcacgcg cggtcgagat gcggcgaatt ctgccggaat cggtgcttga ggaagtgggc
      721 aaactcgatc ccgcggcgat tgcgcaagtg cgccaagaag cgtggccgga cgtgcgtgat
      781 gcggacgaac tgcacgacgt gctgcatact ctgatcgcat tgcccatttc ggctgaaggg
      841 tcgaatacgg accgagctac gctcggttgg acagcccctt cggctacgct cagggcaggc
      901 tcttgcggct gtccctacgt ggccggtgct cattctcagt ggcaaatata ttttcgatat
      961 ttgctcgatc aggggcgggt cacaactgcc caagccgccg atcgcagcta ttgggttacc
     1021 gccgagcgcg ccaacgcttt cgcgctgcta tttccagacg cgacgttcat cccacaactc
     1081 gccgaaattg atggaacgct tcccatccgc gaagatgcaa tgctggcgct cgttaccgga
     1141 tggatgtcgc atataggtcc agttaccgca aaggagctgg cgtcgctgct ggggctgtcg
     1201 tcttctgagg tcgaccaagc tctgcttcgc atggaagcca gcggggcgat tcttcgcggg
     1261 aagtttaccg ataaagcatc gcgggcgggg gccccattcg acttcgctca gggcaggtcc
     1321 gcgccacata agcaagagac cgaatggtgc gaccggcgat tgctggcccg aatccatcgc
     1381 cttaccgttg cgacgctacg caaacaaatc gagccggtga cggccgc
//
LOCUS       DATCBB010000953         1349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_485659, whole genome shotgun sequence.
ACCESSION   DATCBB010000953 DATCBB010000000
VERSION     DATCBB010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485659"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="rho"
                     /locus_tag="VII37_10435"
     CDS             <1..1094
                     /gene="rho"
                     /locus_tag="VII37_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5070201.1"
                     /translation="LEVLPEGFGFLRSQSFNYLPCPEDIYISPSQIRRFDLQTGNLIA
                     GQIRPPKEKEKFFALLKVEAVDGEEPDKAKDKTHFENLTPLFPNKRFILETAPEELST
                     RVLDLVCPIGKGTRGLIVAPPRTGKTVLMQKMANAILKNNPETYLFILLIDERPEEVT
                     DMERSCKGAEVISSTFDEPPERHVQVAEMVIEKAKRMIEQKRDVVILLDSITRLARAY
                     NTVQPHSGKILSGGVDANALHKPKRFFGAARNIEEGGSLTIMATALVDTGSRMDEVIF
                     EEFKGTGNMEVHLDRALVDRRIFPSINIERSGTRKEELLYHPDEYNKVVLLRRALTGV
                     PPVEAMELMLGKLKKTRSNIEFLLNMGVG"
     gene            complement(1160..>1349)
                     /locus_tag="VII37_10440"
     CDS             complement(1160..>1349)
                     /locus_tag="VII37_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070202.1"
                     /translation="AVMIDMTMNASFKDALLELNAEAGKTYYIQFKIATTWGPKLVEM
                     DADTGAREIAKCRLAKPL"
BASE COUNT          328 a          356 c          376 g          289 t
ORIGIN      
        1 tgctggaagt gctgccggaa ggttttggat ttttgcggtc gcaaagtttc aattatcttc
       61 cctgcccgga ggatatttat atttcgccgt cgcaaatccg ccggttcgat ttgcagacgg
      121 gcaatttgat cgcaggccag atccgtccgc cgaaggaaaa ggaaaagttt ttcgcgctgc
      181 tcaaagtcga ggccgtggac ggcgaggaac cggacaaggc gaaggacaag acgcattttg
      241 aaaacctcac gccgctgttc ccgaacaaac ggttcatcct ggaaaccgcg cctgaggaat
      301 tgagcacgcg agtgctggac ctggtttgtc cgatcggcaa aggcacgcgc ggtttgatcg
      361 tcgccccgcc gcgcacgggc aagacggttt tgatgcagaa gatggccaac gccattttaa
      421 agaacaaccc ggagacttat ctattcatcc tgctcattga cgagcggccg gaagaagtga
      481 cggacatgga acgttcctgc aaaggcgcgg aagtcatcag ttcaacgttc gacgaaccgc
      541 cggagcgcca tgtgcaagtc gccgaaatgg tcatcgaaaa agccaagcgc atgatcgaac
      601 aaaaacgtga cgtggtgatt ttgctcgatt ccatcacgcg ccttgcccgc gcttacaaca
      661 cagttcagcc gcactctgga aaaattcttt ccggcggtgt ggacgcgaac gccttgcaca
      721 agccaaaaag atttttcggc gcggcgcgca acatcgagga gggcggttcg ctcacaatca
      781 tggccaccgc gctcgtggac acaggttcgc gcatggacga agtgatcttt gaagaattca
      841 aaggcacggg caacatggaa gtgcatttgg accgcgcgct ggttgaccgc cgcatttttc
      901 cgtccatcaa catcgagcgt tccggcacgc gcaaagaaga attgctgtat catccggacg
      961 aatacaacaa ggtggtcctg ctccgccgcg cgctcacggg tgtgccgccg gtcgaggcga
     1021 tggaactgat gctgggcaaa ctcaagaaga cccggagcaa catcgaattc ctgctcaaca
     1081 tgggcgtggg ttgattcaac gaggcgcagc aaaggattaa gaaaaggcgt tgggcgagga
     1141 gattttgccc aacacttttt tacaggggtt ttgccaatct gcacttcgcg atttcgcgcg
     1201 ctccagtatc agcgtccatt tccaccaatt tcggacccca ggtggtggcg attttaaact
     1261 gaatgtaata cgtcttgccc gcctccgcgt ttaattcgag taaagcgtct ttgaaggatg
     1321 cattcatggt catatcaatc atcacagcg
//
LOCUS       DATCBB010000954         2704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_225203, whole genome shotgun sequence.
ACCESSION   DATCBB010000954 DATCBB010000000
VERSION     DATCBB010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225203"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VII37_10445"
     CDS             complement(<1..579)
                     /locus_tag="VII37_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEY5070203.1"
                     /translation="MQTAATPGSEIGVQTDWRAKKLHALGVFCGFTAGAWLGTAEAPT
                     KLVSIGVSPLVISLIMVIGVFLARWSLPALMVGTSSISADVRRSPHLIVWALLAGCMW
                     AVANTLTIFAIRDIGLSIAFPLWNSNSLLGIFWGFVFFRELRQAGWRRWTGVLGGALI
                     MCVGATLLAIASSTQAPAGHSMRGVWAALGAGI"
     gene            complement(812..1288)
                     /locus_tag="VII37_10450"
     CDS             complement(812..1288)
                     /locus_tag="VII37_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEY5070204.1"
                     /translation="MSASEILLLAFLLGMVAGLRSLTAPAVLAWAAHRNWLNVHNTSL
                     SFMASTAAMVIFVLLAVGELIADQLPSTPSRTAPPGLIARIVMGGLCGAGIAAAGTQS
                     LVLGAILGIVGALIGTFGGYQVRTRVVTAFKVPDLVIATLEDVVAIGSGLFIVSRF"
     gene            complement(1359..>2704)
                     /locus_tag="VII37_10455"
     CDS             complement(1359..>2704)
                     /locus_tag="VII37_10455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5070205.1"
                     /translation="FSRRIGEKARSIPKGEWILGGDWDETKWNPAALPTNKTIDALTP
                     DTPVFVSRYDGHMALANSLAMKLAGINKSTPDLAGGTVVRDAQGNPTGALKDAAQSYM
                     RSALPVPSHEQRTKAAKRALAHAASVGVTSVQDMYTDFANIAVYAELLEHGQLTARIY
                     AAPSVALVEDQARLGVHHAFGGPYLRIGAVKGFADGSLGSRTAYFFEPFNDQPGNHGL
                     LSDTMQPLSRMRDYMMTADSAGIQICTHAIGDQAISTVLDLYAEVAKAHGPADRRFRI
                     EHAQHMAAKDFDRFDQLSVIASVQPYHAIDDGRWAEPRIGHERASRTYAFRTFLNHGV
                     RLAFGTDWDVAPLNPMLTIYAAVTRATLDDKNPGGWFPEQKLTVAEAIEAYTMGSAYA
                     EFQEKTKGSITPGKLADMVLLSDDILSIDPVKIRDVKVLMTIVGGKIVFDVNPSN"
BASE COUNT          642 a          806 c          702 g          554 t
ORIGIN      
        1 tattccggcc cccagagccg cccacacgcc acgcatggaa tggcctgccg gagcttgcgt
       61 tgaagaagca atcgccaata atgttgcgcc tacacacatg atcagcgcgc cgccaaggac
      121 tccggtccaa cggcgccagc ccgcttgacg caactcgcga aagaaaacga aaccccaaaa
      181 tatgcccaaa agactgttcg aattccacag aggaaacgcg atgctcagcc cgatgtcgcg
      241 tatagcaaaa attgtcagcg tattcgccac cgcccacatg catccggcca gcaaggccca
      301 aacaatcaga tgtggtgagc gtcgaacgtc cgcacttatg gatgatgtcc ccaccatcaa
      361 agcgggcaag ctccaacgag ccaggaacac gccgatcacc atgatcagcg agatcaccag
      421 tggcgaaact ccgatcgaaa caagtttagt cggcgcctcc gcagtgccta gccaagcgcc
      481 ggctgtaaaa ccgcaaaaca ctcccaaggc gtgcagcttc ttcgctcgcc aatcggtttg
      541 gacccctatt tcagaaccgg gcgttgccgc cgtttgcata tttctccgaa tttaaaatct
      601 caactgatcg atctcaggat cagcaggccg cctataagca ccgcgagagg tacccaaaaa
      661 tgaatgtccg tcagaatgga gatgatcagc tttcgtgtgg atgatgtact tgcgactcgt
      721 tgcacatttt tctccatgcc ctaaggtctg agtggaagga tttttaacgc gcagctcacg
      781 cccgcgcgcg aaaaagcctg gcgtaatttc gctagaaccg cgagacgatg aacaggccgc
      841 tgccaatcgc cacgacatct tcaagcgtgg cgataaccag atcggggact ttgaaggccg
      901 tcactactct ggtccggacc tggtaaccgc cgaacgtgcc gataagcgcg cccacgatac
      961 ccaaaattgc gcccagcaca agtgattgcg ttcctgccgc cgcaatcccc gcgccgcata
     1021 gcccgcccat cacaatgcgc gcaatcaatc ccggcggcgc cgtgcggctg ggagtggagg
     1081 gaagttggtc ggcgatcaat tcgccaaccg ccaacaaaac aaaaataacc atcgcagcgg
     1141 tggaggccat aaacgagaga gacgtgttgt gtacattgag ccagttgcga tgagcggccc
     1201 acgccaagac ggcaggcgcg gtcaaagagc gcaagcccgc gaccatacct agaagaaacg
     1261 ctaacagcag aatctctgaa gcactcatcg acaaatcttt ccgacacgcg cggttttcga
     1321 cttcgccacg gcagttgctg agttcggcgc agcttcggtt aattgctggg attcacatca
     1381 aaaactatct tgcccccaac tatagtcatc agcactttca cgtcacggat ttttactgga
     1441 tcgatagaca aaatgtcgtc gctcaacaac accatgtcag cgagcttacc cggagtgatg
     1501 gatcccttgg ttttttcctg aaactccgcg tatgccgacc ccatggtgta agcctcgatc
     1561 gcttccgcaa cagttaactt ctgctctgga aaccaacccc ccggattttt atcgtccaaa
     1621 gttgcgcggg ttaccgccgc atagattgtc agcatcggat ttaaaggagc aacatcccag
     1681 tccgtaccaa acgcgagtcg cacaccatga ttcaaaaatg ttcgaaacgc atacgtgcgg
     1741 ctggcgcgct catgtccgat gcgtggttcg gcccagcggc catcgtcaat cgcgtgatat
     1801 ggctgaacgg aggcgattac acttagttgg tcaaaacggt cgaaatcttt tgccgccata
     1861 tgttgcgcgt gttcgatccg aaagcggcgg tccgcgggcc cgtgcgcttt tgcaacttcg
     1921 gcatagagat cgagaaccgt agatatggcc tggtcgccaa tcgcgtgcgt gcaaatctgg
     1981 atgcccgcgc tgtccgcagt catcatgtaa tcgcgcatcc gcgacagcgg ctgcatggta
     2041 tccgatagta agccgtggtt cccgggttgg tcgttgaatg gttcaaaaaa atatgccgtg
     2101 cgcgagccca aagagccgtc cgcaaatccc ttcaccgctc cgatgcgcaa atacggaccg
     2161 ccaaaagcat gatgcacccc caaccttgcc tggtcctcca ccaaggcgac ggacggagcg
     2221 gcataaatcc gggcggttaa ctggccgtgc tccaataatt ccgcgtatac cgcgatattc
     2281 gcgaaatctg tatacatatc ctggacgctg gtgacgccca cggacgcggc atgggccaat
     2341 gcccgtttgg cggcttttgt tcgttgctcg tgcgacggca ccgggagagc actgcgcata
     2401 taactctggg ccgcatcttt cagagcaccg gtgggattcc cttgcgcatc ccgtaccact
     2461 gtgccccccg caaggtctgg ggtggattta ttgatgcctg caagtttcat tgccaaagaa
     2521 ttcgccagcg ccatatggcc gtcatagcgg ctcacaaata cgggcgtatc gggggtgaga
     2581 gcatcaatgg ttttgttcgt cggcaatgct gcggggttcc atttggtttc atcccagtct
     2641 ccgccaagta tccactcgcc cttgggaatc gagcgcgctt tctcgccgat gcgtcgcgaa
     2701 aatt
//
LOCUS       DATCBB010000955         1223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_255889, whole genome shotgun sequence.
ACCESSION   DATCBB010000955 DATCBB010000000
VERSION     DATCBB010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255889"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VII37_10460"
     CDS             complement(<1..1033)
                     /locus_tag="VII37_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY5070206.1"
                     /translation="MRDPVELEVFRNLYHSIAEEMGATLRRTSFSPNIKERRDYSCAV
                     FDSLGQVIAMGDHMPVHLGSMPMSVAAAIKQCALEPGDMVMLNDPFRGGTHLPDITLV
                     MPVYLNQDSSRGDGAPPRQGGAKPRRHTNNPDFYVASRAHHADVGGMYPGSMGPCREI
                     YQEGLRIPPVKIMRDGKIIADVLALLLNNVRTPEEREGDLGAQIAACETGAQRLREIC
                     SRYGISRAKKAAANLLLYSEKLMRAFLQTIPPGHYQAEDFLDDDGVESKPVRIAVTIH
                     VARANKKSSVETRLAASHSSSSKKPQVTIDFTGSDPQVQGAVNAVEAITYSACFYVFR
                     CLLNDDVPAT"
     gene            1032..>1223
                     /locus_tag="VII37_10465"
     CDS             1032..>1223
                     /locus_tag="VII37_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070207.1"
                     /translation="MGADEFTPKRSHMTKGLRCYTRLIMAEGRSRIWIWLLVGGGLFA
                     LFVVAVFTLVYLSFNGRDEE"
BASE COUNT          240 a          305 c          395 g          283 t
ORIGIN      
        1 aggtggcggg gacatcgtca ttcagcaggc agcggaagac gtagaaacac gctgagtagg
       61 tgatggcttc gaccgcgttg accgcgccct ggacttgcgg gtcgctgccg gtgaagtcga
      121 tggtgacctg gggcttcttg ctggacgatg agtgagacgc agctagccgc gtctctacag
      181 aggatttttt gtttgcccgg gcgacatgga tggtcacggc tatacgcacc ggcttgcttt
      241 ctactccatc gtcatccaga aaatcttctg cctgatagtg gcccggaggg atggtttgca
      301 agaaagctcg catcaatttt tctgaataga ggagcaggtt ggcggctgct tttttggcgc
      361 gggagattcc atagcggctg cagatctcgc ggaggcgttg cgctccggtt tcgcaggcgg
      421 caatctgtgc tcctaaatcg ccttcgcgtt cctctggcgt gcgcacgttg ttgagaagaa
      481 gggcgagcac gtcggcgata atttttccgt cgcgcatgat tttgacgggc ggaatgcgca
      541 ggccttcctg atagatttcg cggcacggcc ccatggagcc tggatacatt ccaccaacgt
      601 cggcatgatg ggcgcgagaa gcaacgtaaa aatcggggtt gtttgtgtgg cggcggggct
      661 ttgccccgcc ttgacggggc ggagccccgt caccacgcga actatcctga ttgagataaa
      721 ccggcatgac cagcgtgatg tcaggaaggt gcgtgccgcc acggaaggga tcgttgagca
      781 taaccatgtc accgggttcg agcgcgcatt gcttgatcgc ggctgcaacc gacatcggca
      841 tggatccaag gtgtaccggc atgtgatccc ccatcgcgat cacctgtccc aaggaatcaa
      901 acacggcgca ggagtagtcg cgacgctctt tgatgttggg ggaaaaggaa gtgcggcgca
      961 gcgttgctcc catctcttcg gcgatggagt gataaagatt tctgaagact tcgagttcaa
     1021 ctgggtcgcg catgggcgct gacgagttta cgccaaaacg cagccatatg accaaaggcc
     1081 tgcggtgcta cactcgtctg attatggctg aagggcgttc gcgaatttgg atttggcttc
     1141 tggtgggcgg cggcttgttt gcgctgttcg tggtggcggt attcacgttg gtatatttga
     1201 gtttcaatgg ccgcgacgaa gaa
//
LOCUS       DATCBB010000956         3975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_160404, whole genome shotgun sequence.
ACCESSION   DATCBB010000956 DATCBB010000000
VERSION     DATCBB010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160404"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..634
                     /locus_tag="VII37_10470"
     CDS             212..634
                     /locus_tag="VII37_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5070208.1"
                     /translation="MKIGELATRTGVNASAIRYYEKLSLVPAPYRLSGQRRYTDEALY
                     RVLLIRFARDMDFSLAEIKIFLSGLNDKTPVGKRWKRLAHSKIKQVDETVLRARRLKS
                     LLEHLLQCRCASLRQCVDRLSLSPKLRLVRNGDEQRQH"
     gene            complement(668..1060)
                     /locus_tag="VII37_10475"
     CDS             complement(668..1060)
                     /locus_tag="VII37_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5070209.1"
                     /translation="MHIPENSTLARIGQIAVNVHDVDRATAFYRDVLALPHLFRAGHL
                     SFFNCGGVRLMLSPPEKPQFDHPASILYFQVADIRAAYERLKSAGVKFEDEPHLIARM
                     PSHELWMNHFHDTEGNLLALMSEVPNAA"
     gene            1110..1565
                     /locus_tag="VII37_10480"
     CDS             1110..1565
                     /locus_tag="VII37_10480"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5070210.1"
                     /translation="MARRKIKISSETEAYRIGDLLHSAAIHLLRKVRAQDRSAGIGPA
                     QLSALSVLVFGGPRSLKELAEAEQVRPPTMSRIVVGLERTRLVRRKTTNDKRRMLLEA
                     TARGAKILHEGRRRRVEVLVHALQEFSEDEQREAAAAAEFMRKLVGKLS"
     gene            1595..2209
                     /locus_tag="VII37_10485"
     CDS             1595..2209
                     /locus_tag="VII37_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY5070211.1"
                     /translation="MPDSTRLTPDAFAMWKINARLGVLAAIFVGIPLRLLAQQETGAV
                     EVVVNDQQGAGIAEAQIEVVPRPENSAQKLKTDKKGHASLRLNAGEYALFISAQGFKS
                     SAQALSLGNRDGAGETNQVVKVILKVADTGSPTIIYPPDSLVLVGDPYRAPVVLTSAD
                     FRALPHTTIKVHNGHTNAEEIYSGVLLETLLAKANAPVGKESRK"
     gene            2434..3909
                     /locus_tag="VII37_10490"
     CDS             2434..3909
                     /locus_tag="VII37_10490"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEY5070212.1"
                     /translation="MTAAKKSQKTRSEPEKAAGVSQRNVLPGPTLKEKLAIGKALRRR
                     ATRSAQGKWVPPANRFDPIELLKKSDHGRLPELLPIRYSRMRQSAFGFFRGAAAVMAA
                     DLATTPRTGLHVQACGDCHVSNFGGFGSPERRLVFDINDFDETSHAPWEWDVKRLATS
                     IVLAGRQKGDGQHFCKKAVRAAVASYREHMNQYAKMRALEVWYSALDAEILISSAKTK
                     KAKKYWERIEAKAKLQTAEHVFPRMTQRVNGLPRIIDNPPLIYHPRQRIKQGKRVREM
                     FRRYRLTLPEERRVIIDRYHIVDIARKVVGVGSVGTRCAVVLLMADKGDPLFLQFKEA
                     LPSVLEPYAGKSRYSYHGERVVTGQRMLQAASDIFLGWTRDDQGHDYYFRQLRDMKMT
                     INVEDMPTEDWIEYLELCGWTLARAHARTGDAAQIAGYLGSSESFDEAVAKFAFAYAD
                     QTQRDHEMFVKAVRSGRIKASRRLRVEGFLMEPRNPASRQP"
BASE COUNT          992 a          954 c         1182 g          847 t
ORIGIN      
        1 atccggcagt gggtccatta atgaccacaa gcgctaacgt ttgtcggtgc taatccgaca
       61 aaaaaccgat aaaggttcgc acacctgtca cccagcggaa atgctggcca agcagtgccg
      121 acctagattt gcgacatcgt acagagcaat ggtaaaagtt gaagttagct ttaagtcaag
      181 aagcgtttag ccgccaaact ccgaggtgaa catgaaaatt ggagagctgg ccacccgaac
      241 gggggtgaat gcctcagcaa ttcggtacta cgaaaagctg agcctggttc cggctcctta
      301 tcgtttgagc ggacagcggc gctataccga tgaagcgctt taccgcgtgc tgctcatacg
      361 gttcgcgaga gacatggatt tttcattggc ggagattaag attttcttga gcggtcttaa
      421 cgacaaaacc ccggtaggca aacgttggaa gaggctcgct cattccaaaa tcaaacaggt
      481 tgatgagacc gtgctgcgcg cccgacggct caaatcactt ctggagcatc ttttgcaatg
      541 tcgatgcgcc tcgttgcgac agtgtgttga ccggctcagt cttagcccga agttgcggct
      601 cgtgcgcaac ggcgacgagc agcgccagca ttagttgtcc gggagcacgg aggtcgcgac
      661 gaaatatcta tgcggcgtta ggaacttcgc tcatgagcgc gagaaggttg ccttccgtgt
      721 catggaagtg gttcatccac agctcgtgag acggcatgcg ggcgatgaga tgcggctcat
      781 cctcgaattt tactcccgcc gatttaagac gttcatatgc cgcgcgaatg tctgcaacct
      841 gaaaatacaa aatcgaagcg ggatggtcga attgaggttt ttctggcggg ctgagcatca
      901 ggcgcacgcc gccgcaatta aaaaagctta aatggccagc gcggaagagg tgcggcaaag
      961 cgagcacatc gcgatagaat gcggtggcgc gatcgacgtc atggacgttg acggcgattt
     1021 gtccgatccg ggcgagagtg gagttttcgg gaatgtgcat ggagaagccc ccctcaaata
     1081 gaattagtta gcatagctaa ctatatttca tggccagacg caagattaaa atttcttcgg
     1141 aaacggaagc ctaccgcatc ggagacttgt tgcattccgc tgccattcat ttgctgcgta
     1201 aggtacgcgc gcaagaccgc agcgcgggga ttggaccggc ccagctttcc gcgctttcgg
     1261 tgttggtatt tggcgggcca cgttcgttga aagaattggc ggaggctgaa caagtgcggc
     1321 ctccgacgat gagtcgtatc gtggttgggc tagagcgaac ccgtctggtg cgacgcaaaa
     1381 ctaccaacga taaacggcgt atgctcctgg aggccacggc gcgcggcgcg aagattctgc
     1441 acgagggcag gcgacgccgc gtggaagtgc tagtacacgc cttgcaggaa ttttccgagg
     1501 acgaacagcg agaagcagcc gctgcggcgg aatttatgcg gaagcttgta ggaaaattat
     1561 catgacaaga agggctgcaa tttcagcggt acatttgcct gacagcaccc ggcttacgcc
     1621 cgatgctttc gccatgtgga agataaacgc cagattgggg gtgctcgcgg cgatattcgt
     1681 cggcattcct ttgcgtctat tggcgcagca ggagactggc gccgttgaag tggtagtaaa
     1741 cgatcagcag ggagctggta tcgcagaggc ccagatagaa gtggttccgc gtccagaaaa
     1801 ctccgctcag aaattaaaaa cagataagaa gggacacgct agcctgcggt taaacgcggg
     1861 cgagtatgcc ttgttcattt ccgcgcaagg atttaagtcg tctgcgcaag ctctttcgtt
     1921 aggtaatcgt gacggcgcgg gcgaaactaa tcaggttgta aaagtgattt tgaaggtcgc
     1981 ggacaccggt tcgccgacca ttatctatcc ccctgattct ctggtcctag tgggcgatcc
     2041 gtatcgtgcg cccgtggtgc tgacatcggc ggattttcgc gcgctgccac acactacgat
     2101 caaggttcat aacggacaca ccaatgcaga ggaaatttat tcgggcgtgt tgctggaaac
     2161 attgctggcg aaggcaaacg cgccagtcgg caaggaatct cgcaagtagg cgttgagtag
     2221 ttatctcgtt gtgtcgggac ctgacggata ctccgtggtg ctttctcttg cggaaataga
     2281 ttccgctttt catgaggggc aagtgattgt ggctgatacg cgcggcggtc aacagcttgg
     2341 caagtacggt ccctttcaac tggtagtgtc aggtgatagg cgcccggctc gctgggtgca
     2401 taatctcaat cttatacagc tcaaacaggc tcaatgaccg cggcgaaaaa gtcccaaaaa
     2461 acaagaagtg aacctgagaa ggccgctggg gtttcacagc gcaacgtcct tccaggaccc
     2521 acgctaaaag agaaacttgc catcggcaaa gcgctgcgta ggagagcgac gcgaagcgcg
     2581 caagggaagt gggtaccgcc ggccaatcgt tttgatccta ttgagttgtt gaagaaatca
     2641 gaccatggaa gattgccgga gcttttgccg atacgctact cgcggatgag acagtcggcg
     2701 ttcggatttt ttcgcggcgc agctgcggtg atggcggcgg acctggctac gactccacga
     2761 acaggattac atgttcaggc ttgcggcgat tgtcacgtca gcaattttgg cggattcggt
     2821 tctccggaga ggcggctggt ttttgacatc aacgattttg acgaaacgtc gcacgcgcca
     2881 tgggaatggg acgtcaagcg gctggccacc agcatcgtac ttgccggaag acaaaaaggt
     2941 gacggacaac atttttgtaa aaaagccgtg agagcggcag tggcgagtta tcgggaacat
     3001 atgaaccagt acgcgaaaat gcgcgcactg gaagtgtggt actcggcgct cgacgcggag
     3061 attttgataa gcagcgcgaa gacgaagaaa gccaagaaat actgggaacg catagaggcg
     3121 aaagcaaaat tgcaaaccgc ggagcatgtt tttccgcgta tgacgcagcg cgtgaacggc
     3181 ctaccgcgca tcatcgacaa tccgccgcta atttatcatc cgcgtcagag gatcaaacaa
     3241 ggcaagagag tacgggaaat gtttcggcgc taccgcttga cgttgccgga agagcggcgc
     3301 gtgattatcg accgctacca cattgtggac atcgcacgaa aagttgtcgg cgtcggaagc
     3361 gtcggtacga gatgcgcggt ggttttgctt atggccgaca agggtgaccc gctatttttg
     3421 caatttaagg aggcgcttcc gtcggtattg gaaccatatg ccggtaaaag tcgctatagc
     3481 tatcatgggg aaagagtggt caccgggcag cgcatgttgc aggcggccag cgacattttt
     3541 ctgggttgga cgcgtgatga tcaaggccac gactattatt ttcggcagct gcgagacatg
     3601 aagatgacca tcaacgtgga agatatgccc acggaagatt ggatcgagta cctcgaactt
     3661 tgcggatgga cattagcgcg agcgcatgcg cgtaccggag atgccgcgca aattgccggg
     3721 tatttgggaa gcagtgaatc attcgatgaa gctgttgcga aatttgcctt cgcctatgcg
     3781 gaccagacgc aacgggacca tgagatgttt gtgaaagcag ttcgcagcgg aaggattaaa
     3841 gctagccgaa ggttgcgggt tgaaggcttt ctgatggaac cacggaatcc tgcaagcaga
     3901 caaccataaa cgaaattgta actcccgcaa aaaactaaca gcaaaaactc ggacccgtcc
     3961 caaaaatcga gagga
//
LOCUS       DATCBB010000957         1100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_450354, whole genome shotgun sequence.
ACCESSION   DATCBB010000957 DATCBB010000000
VERSION     DATCBB010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450354"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..>1100
                     /locus_tag="VII37_10495"
     CDS             159..>1100
                     /locus_tag="VII37_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5070213.1"
                     /translation="MKKPYQIESQRAVKRLEEMAAEGNPTVQMVLPMAEMIGWLRKGV
                     GELIRQAGLQIIGLLMDEEVGQLVGERSRPSPERKANRWGTERGYCVVMGQKVPVERP
                     RVRSTDDREIRLGSYELFHRGEPLTETVWEKLMLGLTTRKYGEAVRQFTEAYGLEKSA
                     VSEHFIESSRAKLKELMERRLNKMKFCALLIDATPFEGQQMVAALGIGYDGTKTILGI
                     RQGATENATVVGELLGDLMNRGLDFTEPRIYVLDGGKALHSAVKKYAGEAAPIQRCQV
                     HKRRNVLDHLCDGDKSIIAQKLNAIYAMEDYTAAKTAL"
BASE COUNT          268 a          278 c          369 g          185 t
ORIGIN      
        1 ccgcagcgcg gacagtgcgg gacgttagtg gccatttgct gagggctttc cgagtgtgac
       61 cgggtggact gtttcaaagc gcgaccaacc gggatcagcg ggtctggatt tgataggctc
      121 agagcaattt ttcttgacgg aaaaaaggag agtcgctaat gaagaaacca tatcagattg
      181 aatcgcagcg agccgtgaag aggctcgaag agatggccgc agagggaaat ccgacggtcc
      241 agatggtgtt gccgatggcc gagatgatcg gctggctgcg caaaggagta ggcgaactga
      301 ttcgccaggc cggattgcaa ataatcggac tgctcatgga cgaggaagtg gggcaactgg
      361 taggcgagcg cagccgcccg tcaccggagc ggaaagcgaa tcggtggggc accgagcgcg
      421 gctactgcgt agtgatgggg caaaaggttc cggtcgaacg accgcgcgtt cgttcgaccg
      481 acgaccggga gatccggctg gggagctacg aattgttcca ccggggcgag cctctgacgg
      541 aaacggtttg ggagaaactc atgctcggcc tgaccacgcg gaaatacggt gaagccgtgc
      601 gccagttcac cgaagcttac gggctggaaa agagcgcggt gagcgagcac ttcattgaat
      661 ccagccgggc aaaactgaaa gaactgatgg agcggcggct gaacaagatg aagttctgcg
      721 cgctgctcat tgatgccact ccgttcgagg gccagcaaat ggtggccgcg ctgggcatcg
      781 gctacgacgg cacgaaaacg attttgggta tccgccaggg cgccacggaa aacgcaaccg
      841 tggtcggcga actgctgggc gatctgatga atcggggact ggacttcaca gaaccgcgca
      901 tctacgtgct ggatggcggt aaagcgctgc attcagcggt gaagaaatat gccggcgaag
      961 cggcgccgat acagcgttgc caggtacaca agcggcgcaa cgtcctggac catctgtgcg
     1021 acggcgacaa atccatcatc gcccaaaagc tgaatgccat ttacgcgatg gaagactata
     1081 cggcggcgaa aaccgcactc
//
LOCUS       DATCBB010000958         1659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_450363, whole genome shotgun sequence.
ACCESSION   DATCBB010000958 DATCBB010000000
VERSION     DATCBB010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450363"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VII37_10500"
     CDS             complement(<1..335)
                     /locus_tag="VII37_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070214.1"
                     /translation="MSDIVIRKCQTLDDFHLCVALQREIWQEADLEIEPVTAFVVAAN
                     TGGQVLGAFEGARLVGYTLAVAAIRDGVLYLHSHMAGVHADFRDRSIGRLLKLAQRDE
                     ALSRNLRLI"
     gene            complement(332..1333)
                     /gene="mazG"
                     /locus_tag="VII37_10505"
     CDS             complement(332..1333)
                     /gene="mazG"
                     /locus_tag="VII37_10505"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY5070215.1"
                     /translation="MRSPTKKSLKSQKKTATRKDPARKSARPNTISAGEWFEKLVAVQ
                     KRLRAPQGCPWDREQTHATLRPYLIEEAYEVIDALDSGDDAKFAEEMGDLLLQVVFHS
                     EIAREQERFSVADVVREIHDKMVRRHPHVFGKVRARNSAEVLQNWERIKATERLDKDN
                     SSKNGASTSQQKPTSLLDGVSAGLPAMLQGLQLTRKAARAGFDWDHADGIFEKLREEM
                     AEVRHALAEKDSKKAEEELGDLLFAAVNLARFVEVDPEIALKKANAKFRRRFMEMEKA
                     ALQKARFFDELPREEKESLWDAVKHAEKKNVTADKLANAELQPMNAKNSRMSASNRR"
BASE COUNT          334 a          452 c          475 g          398 t
ORIGIN      
        1 cagatgaggc gaaggttgcg gctgagagct tcgtcgcgct gggcgagttt cagcaaacgt
       61 ccgatgctac gatcgcgaaa atcggcgtga acgccagcca tgtgcgagtg caggtagaga
      121 accccgtcgc gaatggcggc gactgccagc gtgtaaccga ccaagcgcgc accttcaaaa
      181 gcgccaagaa cctggccacc ggtgttggcc gctacgacaa aggcggtgac cggttcgatt
      241 tcgaggtcgg cctcttgcca gatttcgcgt tgcaacgcca cgcacaggtg aaaatcgtcg
      301 agcgtttggc acttgcggat gacgatgtcg ctcatcgtct gttggaggcg ctcatgcggg
      361 agtttttcgc attcatgggt tgtaattcgg cgttggcaag tttgtcggcg gtgacgtttt
      421 ttttctcggc gtgtttcact gcgtcccaca acgattcttt ttcttctcga ggcaattcgt
      481 cgaagaagcg agccttttgc agcgccgctt tttccatctc catgaagcgg cggcgaaatt
      541 ttgcgttggc ctttttcaac gcgatttccg gatcaacctc cacaaacctg gccagattca
      601 ccgccgcaaa cagcagatct cccagctctt cttcagcttt tttggaatct ttttcggcca
      661 gcgcatgccg aacctctgcc atttcttcgc gcaatttttc gaagatgccg tcggcatggt
      721 cccagtcgaa ccctgcgcgg gccgctttgc gcgtcagttg cagaccttgc agcatggcag
      781 gcagtccggc agaaacgccg tcgagcagcg acgtgggctt ctgttgcgac gttgaggctc
      841 cgttcttgga agaattgtct ttgtcgagcc gttctgtggc tttgatccgt tcccaatttt
      901 gcagcacttc agcggagttc ctggcgcgaa cttttccgaa cacgtgcggg tgacgccgca
      961 ccatcttgtc gtgaatttcg cggaccacat ccgcaacact gaaacgctcc tgttcgcggg
     1021 caatttcaga atgaaaaacg acttggagca aaaggtcgcc catctcctcg gcaaacttgg
     1081 cgtcgtcacc ggaatcgaga gcgtcgatca cttcataagc ttcttcaatc aagtaggggc
     1141 gcaatgtcgc gtgggtctgc tcgcggtccc aagggcagcc ttgtggcgcg cgaaggcgct
     1201 tttgcacggc gacgagtttc tcgaaccact ctccggcgga gatcgtgttg gggcgcgcag
     1261 atttgcgtgc tggatctttt cgtgttgcag ttttcttctg cgacttgagt gatttttttg
     1321 ttggggagcg catcgctgtc cgcagaattt cttttaccgc ggggttccca ctttacggcg
     1381 gtcaggaaat tttgacaagg gtactcgcag cgttgtctgc gcgagccagg cttgcatcgc
     1441 cagccgccac catagctaaa cggaactggc gtgataaact acaattcaca ttaacgatag
     1501 agggactaca ttgccagaag ataagcaaga agaggctttt cgcgtaatcg acaggcggcc
     1561 ttttacggcc gaaggcgaaa ttcgcaaaga cgtggttgaa gcggaagaac gtaaagcgga
     1621 acgcgaggcg caactggaag cggcgcgcca ccctgccac
//
LOCUS       DATCBB010000959         1009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_30258, whole genome shotgun sequence.
ACCESSION   DATCBB010000959 DATCBB010000000
VERSION     DATCBB010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30258"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VII37_10510"
     CDS             complement(<1..983)
                     /locus_tag="VII37_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070216.1"
                     /translation="MAREHRYTEAEAALKGVSAPANPAQKIAFYRLKAAIASGLGHFT
                     AAAADMDKAAALAPGNQDLRVAAGIAHLEEQVENHANPAQTLERLRSETLPPQQAIDL
                     RLHLAEILSRASLFAEAATDFAAASALAPDRADLLYNLALADFRSGHLDDALANAERT
                     KGLEDSASIESLLGDIQEKRGDSLAAAHSYQAAVNLQPSEERYHIALALELLRHQTFD
                     AALVVLNQSDTLFPKSARVKILLGLTYFFVDRSAGAVQALLQAAELEPEDETVARYLG
                     EITLLDTSTPNPEATAQECKFADEHPQNKTADAYCGAVLLRIARDSEDDSRR"
BASE COUNT          188 a          264 c          335 g          222 t
ORIGIN      
        1 cggcgtgaat catcttcact gtcacgagcg atgcgcagta agacagctcc gcaataggca
       61 tcggcagttt tattctgcgg gtgctcatcc gcgaatttgc attcctgcgc cgtcgcttcg
      121 gggttcgggg tggaagtatc cagcaaggtg atctcgccga gatagcgggc cacggtttcg
      181 tcttccggtt cgagttcagc ggcttgcaac aatgcttgaa cggcacccgc cgaacggtca
      241 acgaagaaat acgtcaggcc caagagtatt tttacgcgag cggatttcgg gaagagggtg
      301 tccgattgat tcaggacgac cagggcggcg tcaaaggtct ggtggcggag caattccagg
      361 gctaacgcaa tgtggtaacg ctcttccgag ggttgcagat ttaccgcggc ctgatagctg
      421 tgagccgcag cgagcgagtc tccgcgcttt tcctgaatat ctccaagaag gctttcgatc
      481 gaggcgctgt cttccagccc tttagtacgt tcggcgttgg cgagggcgtc gtccaggtga
      541 ccgctgcgga aatccgccag cgccaggttg tagagcaaat cagcgcggtc gggagcaaga
      601 gcgctggccg cggcaaaatc ggtggcggct tccgcaaaca aactggcgcg gctgagaatt
      661 tcagccaggt ggaggcggag gtcgatggct tgttgaggcg gcaatgtttc gctccgtaag
      721 cgttcgaggg tctgcgccgg atttgcgtgg ttttccacct gctcttccag gtgagcgatg
      781 cctgcggcta cgcgaaggtc ctgatttccc ggcgccagcg cagcggcttt gtccatatcc
      841 gcggcggctg cggtgaaatg gccgaggccg gaagcaatgg cggcttttag gcgatagaag
      901 gcaatttttt gagcgggatt tgcgggagcg gagacgcctt tcagcgcggc ttcggcctcc
      961 gtgtaacggt gttctcgggc caactgaagg gcctgtttta gcgccgcat
//
LOCUS       DATCBB010000960         1281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_321648, whole genome shotgun sequence.
ACCESSION   DATCBB010000960 DATCBB010000000
VERSION     DATCBB010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321648"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VII37_10515"
     CDS             <1..506
                     /locus_tag="VII37_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5070217.1"
                     /translation="GLFSDSWLTMVGVIVFSAAQLEERALVFQSVLDNVRLEEVMLTD
                     FATLSPADTLEDALEKAVHSLQDDFPVVRGSDMVGVISKQRILDALRAEGNGYVQAVM
                     NKIFEVSLRQDTLGSAFRKLTARNSSIIPVVEDQRLIGIVTLQNLMHSMSLLAENRKL
                     RRQALDS"
     gene            complement(588..>1281)
                     /locus_tag="VII37_10520"
     CDS             complement(588..>1281)
                     /locus_tag="VII37_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5070218.1"
                     /translation="GLGTTHLDVDAWGIDVIIGGSQKALMIPPGLAYGSISKRAWQRM
                     ETAKSPRYYFDLRKERKAAAKAETASTPATALFAGMAAALDYVREMGDGNLAAGRDAL
                     IANAEMCAAMTRAGAEALGLKLFAPNYPAAALTAIAAPAGTDSTVICKRFREEFGAVV
                     ANGNAELKGQLFRIAHIGYYDYLDTIGILGALEQVIAEVMGKTVEFGRAVKAAQEVYA
                     RTLVNTRTPSVR"
BASE COUNT          249 a          396 c          363 g          273 t
ORIGIN      
        1 cgggcctgtt cagcgacagc tggctgacca tggtcggtgt catcgtgttt tcggccgccc
       61 agttggagga gcgcgccctc gtatttcagt ctgtgctcga caatgtccgt cttgaagaag
      121 tgatgctcac cgacttcgcc acgctttccc ccgccgacac gttggaagac gcgctggaga
      181 aagcggtcca ttccctgcag gatgatttcc ccgtggttcg aggcagcgat atggtgggag
      241 tgatttcgaa gcagcggatt ctcgacgctt tgcgggcgga ggggaacggc tacgtgcagg
      301 cggtgatgaa caagattttc gaagtatccc tgcgccagga cacgctgggc tcggccttcc
      361 gcaaactcac ggcgcgcaac tccagcatca tccccgtcgt cgaagaccag cgtctgatcg
      421 gcatcgtcac cctgcagaac ctgatgcact ccatgtcgct gctagcggaa aatcgcaaac
      481 tgcgcaggca ggctctcgac tcgtaggaaa ttttcgtcgg tttcatcccg attggaaatc
      541 cctattggaa attgtcatcc tgagcgaagc gaaggacctg ctctttgtca gcggacagag
      601 ggagttcgcg tattaactag ggtccgtgcg tacacttcct gagccgcctt gacggcgcgt
      661 ccgaactcta ccgttttccc cataacctcc gcgatcacct gttcaagcgc gccgagtatc
      721 ccaatcgtat ccagataatc gtaatagccg atgtgggcaa tgcggaagag ctgtcccttc
      781 aactccgcat tcccgttggc taccaccgca ccaaactctt cgcggaaccg tttgcagatg
      841 acggtggaat ccgtaccagc cggtgccgcg atggcggtaa gcgcggctgc ggggtaattc
      901 ggagcaaata atttcagtcc cagcgcctcc gcgccggcgc gcgtcattgc cgcacacatc
      961 tccgcatttg cgatcagcgc gtcccgtccc gccgcaagat tcccgtcgcc catctcacgc
     1021 acgtagtcga gcgccgccgc cataccggca aataaagcgg ttgcgggagt tgaagccgtc
     1081 tccgccttgg ctgcggcctt gcgctccttg cgcagatcga agtaatagcg cggagatttt
     1141 gccgtctcca tgcgctgcca tgcgcgcttg ctgattgagc cataagccaa gcccggcggg
     1201 atcatcagag ctttttgcga gccgccgatg atgacgtcaa ttccccacgc gtccacgtcg
     1261 agatgcgtag tgccaaggcc g
//
LOCUS       DATCBB010000961         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_191555, whole genome shotgun sequence.
ACCESSION   DATCBB010000961 DATCBB010000000
VERSION     DATCBB010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191555"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VII37_10525"
     CDS             <1..1003
                     /locus_tag="VII37_10525"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY5070219.1"
                     /translation="GLTLTTGRPEIAQKILLSFARYVDGGMLPNNFPDAGGRPEYNTV
                     DAALWFFEAVRQYYAATQDTETLARLFPVMAQMIDAHVAGTRYQIHVDPADGLLYAGE
                     PGVQLTWMDAKVGDWVVTPRIGKPVEINALWFNALETMASMAPATKQSAEPFAKLATR
                     VKQSFAKFWNASAGHCYDVIDAPGIGNDAALRPNQIFAVSLPQSPLPTEQQKAIVDIC
                     ARRLVTSYGLRSLAQNEPGYQGHYGGNIRERDGAYHQGTVWGWLLGPFVLAHLRVYAD
                     RAAALSFLEPLGKQIHSHGLGTLSEIFDGDAPFTPRGCIAQAWTVAEVLRAWRAIAG"
     gene            1060..2034
                     /locus_tag="VII37_10530"
     CDS             1060..2034
                     /locus_tag="VII37_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY5070220.1"
                     /translation="MNKLIVAVCFLALTLGLPRTSPAQTAPTFVGVRTFLSARYDPDL
                     DHLKADFAVLGVPFDEGTWGQPGERYGPRDMRENSQEYAHDLTEGFYYIDGDRTVLKG
                     KQWVDVGDVLIYPTVPVETDAKITEAVKKILAKKAFPIILGGDHSITFPVIRAYDIAL
                     TVVHIDAHLDTWTGAKGNLDHASWVLRVAKLPTVKHITQIGMRGIANDQEAAGNAKAI
                     PTKVVTSEEIHRSGINAAIEQIPQSENIYVTFDVDSMDPTLAPGTGTLEPGGLNFQEI
                     DELMKQIPTKGKLVGLDIVEVNPYRDPSGRTAQTAIRLMVDLLAAAFH"
     gene            complement(2072..>2710)
                     /locus_tag="VII37_10535"
     CDS             complement(2072..>2710)
                     /locus_tag="VII37_10535"
                     /inference="COORDINATES: protein motif:HMM:NF036542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070221.1"
                     /translation="LSKVDIPEIRRKMLGHLNVIDEKLGAKVADNLGMTGEALAATPA
                     VKPIDLDPSPALRLYGKYKPTLKGRKVGVLLADGFDAKLKDALLAAIKKEGATAAIIA
                     PKVGGVMDAGGKKMTAEAALSGSPSIMFDAVAVLAGPAGDKSLSAEPDAVSFLMDADR
                     HLKAIALAGVSNLAKKTHVEGVVGVTDLRGNGDVAKFIEFARNGKVWERDNA"
BASE COUNT          612 a          819 c          710 g          569 t
ORIGIN      
        1 tggattgacg ttgaccaccg gacgtcccga aattgcacaa aagatcctct tgtccttcgc
       61 gcgttacgtc gacggcggca tgttacccaa caattttccc gacgccggcg gccgccccga
      121 gtacaacacc gtggatgccg cgctgtggtt cttcgaagcg gttcgccaat attatgccgc
      181 tacacaggat actgaaacgc tggccaggct ttttccagtg atggcccaaa tgatcgatgc
      241 tcacgtggcc ggcacgcgct atcaaatcca cgttgaccca gccgacggtc tgctatacgc
      301 cggcgaaccc ggcgtgcaac tcacctggat ggatgccaaa gtcggcgatt gggtagtgac
      361 gccgcgaatc ggaaagcccg tagagattaa tgcgctgtgg ttcaacgcgt tggaaaccat
      421 ggcgagtatg gctcctgcga caaaacaatc tgctgagccc ttcgcaaaat tagcaacgcg
      481 cgtaaaacaa agtttcgcca aattctggaa cgcctctgcc ggacactgtt acgacgtcat
      541 cgacgcgccg ggaattggta atgacgccgc tcttcgaccc aaccaaattt ttgccgtttc
      601 tttgccgcag agcccgcttc ccacggaaca gcaaaaggcg attgtcgata tttgtgcccg
      661 ccgccttgtc acctcgtacg gcctacgcag cctggcgcaa aacgaacctg gctaccaggg
      721 acattacggc ggcaatattc gagagcgtga cggcgcgtat caccaaggca cggtgtgggg
      781 atggctcctc ggcccattcg tgctggcgca cctacgtgta tatgctgatc gcgctgcggc
      841 gctgagtttt ctcgagccgc tcggcaaaca gattcattct cacggcttgg gcacgctcag
      901 cgaaattttc gatggtgacg cgcccttcac cccgcgagga tgtatcgcac aagcctggac
      961 ggtggcggaa gtattgcgag catggcgcgc tatcgccggc taagcgaaac atcacaatgt
     1021 gatgtaacat ccgccaatta aaacgttaaa ggaaccacaa tgaacaagtt gatcgtagcg
     1081 gtgtgttttc tggcgctgac gctcggtttg ccgcgcacct cacccgctca gaccgcgccg
     1141 acattcgtcg gcgtacgcac ctttctgagc gcgcgctacg atccggatct ggaccacctg
     1201 aaagccgact tcgctgttct gggtgtgcct tttgatgaag gcacatgggg tcagcccggc
     1261 gagcgctatg gaccgcgcga catgcgcgaa aattcccagg aatacgcgca cgatttaaca
     1321 gaggggttct actacatcga cggcgaccgc accgtcttga agggcaaaca atgggtcgac
     1381 gttggggacg tgctgattta tccgacggta cccgttgaga ctgatgcgaa aatcaccgaa
     1441 gcggtcaaga aaatcctcgc caagaaagcc tttccaatta ttttaggcgg agaccacagc
     1501 attactttcc cagtcattcg cgcgtatgac attgcgctta cggtcgtcca cattgacgcg
     1561 catctggata cctggacggg cgcaaagggc aacctcgacc acgcctcatg ggtgcttcgc
     1621 gtggcaaaac tacccaccgt caaacacatc acccagattg gcatgcgcgg gatcgctaac
     1681 gatcaggaag ccgccggcaa cgccaaagcc attcccacca aagtcgtcac ctctgaggaa
     1741 atccaccgca gtggaatcaa cgccgccatc gaacaaattc cacaaagcga aaatatctac
     1801 gtcactttcg acgtggactc catggatccc acgttagccc cgggaaccgg cacgctcgaa
     1861 ccgggcggct tgaactttca agagatcgac gaactaatga aacaaatccc caccaaaggg
     1921 aaactggtcg ggctcgacat tgtggaagtg aatccgtatc gagatccgag cgggcgaacc
     1981 gcacaaaccg cgatcagatt aatggtcgac ttgctagctg ccgcgtttca ctaaagtaga
     2041 ccgacgaatt acatgggtcg aatgcgtcgc gctaggcgtt gtcgcgctcc cagaccttgc
     2101 cattccgcgc gaactcaata aacttagcaa cgtcgccatt accgcgaaga tcggtcacgc
     2161 cgaccacccc ttccacatgt gttttcttcg caagattgga aacgccagcc aacgcaatcg
     2221 ctttgaggtg ccggtcggcg tccatcaaaa agctgaccgc atccggctct gcgcttaacg
     2281 atttgtcgcc tgccggcccg gcaagcaccg ccaccgcgtc gaacataatc gacggcgaac
     2341 cagagagcgc tgcctcggcc gtcatcttct tgccaccagc atccatgacg ccaccaactt
     2401 tgggcgcgat gatggctgcg gtcgctcctt ctttcttgat ggccgcgagt aatgcatctt
     2461 ttagttttgc atcgaaaccg tccgccagca gcacgccaac ttttctgccc ttcagtgttg
     2521 gcttatattt tccatacagc cgcagcgccg ggctcggatc gagatcgatc ggcttcaccg
     2581 cgggtgttgc agcgagggcc tcgccggtca ttcccagatt gtccgcaact ttggccccca
     2641 gtttttcgtc gatcacgttt aggtgcccca gcatttttct gcggatttcc ggaatgtcca
     2701 ctttgctcaa
//
LOCUS       DATCBB010000962         3646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_419791, whole genome shotgun sequence.
ACCESSION   DATCBB010000962 DATCBB010000000
VERSION     DATCBB010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419791"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..1909
                     /locus_tag="VII37_10540"
     CDS             437..1909
                     /locus_tag="VII37_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5070222.1"
                     /translation="MATATTVMEPNVKISATKLLINGQWVNSVSGKTFPSINPSTGEE
                     LTQIAEADAADVDKAVAAARAAFEKGPWRKMTASQRGNLMFKLADLIEKNLDELAQLE
                     ALDNGKPYSVARAADLPLTIACYRYYAGWADKLQGKTIPVNGNYFTYTKHEPVGVVAQ
                     IIPWNFPLLMQAWKLGPALATGCTVVMKPAEQTPLSALRVGELALEAGFPPGVLNLLP
                     GYGPTAGAALARHMDVDKVAFTGSTEVGHLVMKAAAESNLKRVTLELGGKSPNIIFAD
                     ADMDKAVEGSHFALFFNQGQCCCAGSRTFVEDKVYDEFVERSAARAKKRTVGNPFDKN
                     TEQGPQVDQEQFTKVMGYIDSGKQQAKLMAGGNRVGDKGFFIEPTVFSDVKDDMKIAQ
                     EEIFGPVMSILRFKDIGEVVERANKSLYGLAAAVWTKDITKAHAIADNVRAGTVWVNC
                     YDVFDAAAPFGGFKQSGIGRELGEYGLANYTEVKTVTIKL"
     gene            2022..2477
                     /locus_tag="VII37_10545"
     CDS             2022..2477
                     /locus_tag="VII37_10545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY5070223.1"
                     /translation="MRIRAYQPHDFDALYKLDQSCFPRGIAYSKSTLRYFLTLSSAEC
                     LVAQEKDDIAGFISSELDGPLAHIITLDVAEAHRRSGVGSLLLDAAENNVFAQGVRTV
                     FLETVVDNHAAVNFWLRHGYIKEGILKRYYLNRIDGYEMRKTLSSRHGQ"
     gene            2809..>3646
                     /locus_tag="VII37_10550"
     CDS             2809..>3646
                     /locus_tag="VII37_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY5070224.1"
                     /translation="MHDYLMSVVLGIVEGLTEFLPVSSTAHLRIAEYLLNISLQSGYW
                     KMYTIVIQIGAIATLPIYFREHIRQLFSTFPNGERNDRTVFTHPLSLVIIAFVITAGP
                     SYLLTKIIGKNLESLYVMGTALVVGGVVMWLVDWMNSKSEAAGEAAGANSRIHTWRMD
                     NMSLGQAMWIGACQILSGVFPGTSRSMSTITAGQVAGMSRSAALEFSFFLSMPTMAMA
                     VAYSLFKSLRGKDENPIGVAQIDAHQWIVLLIGLVVSFVVAYGAVAWFMGWVRKRGFA
                     PFA"
BASE COUNT          835 a          889 c         1072 g          850 t
ORIGIN      
        1 aagccgactc cccagcagcg atccacaaac gtcgcactta cagcggatga cataactgcc
       61 gtcgcctctc aggttggcta tttcgacgct ccgccaaggc cgcaatccgc gccggaaata
      121 ctgggtgtga tcccggagcg caagatcgaa gaaaactagt tttatggttt ggtcggtgac
      181 tctcggtcaa acgatccaca aaactcatcg ctttcggtaa atctacattt aaacagcttc
      241 ccaagtcgtt ctcctggagg ggacttgcct cgatttgagc aggggtatag gcttcttttt
      301 gccggctcat tcttcgagct aagggtctga cgcgcgatgg ccaagtggcc ctcggcgaca
      361 gacaagcagg ctttacaagc ggcttcgcca ctgcaacatt gaaatagata aagattcaca
      421 tacaggaggt tgaaaaatgg ctacagcaac gaccgtgatg gaaccgaatg tcaagattag
      481 tgccactaaa cttctaatta acgggcagtg ggtaaacagc gtttcgggga aaactttccc
      541 gtcaatcaat ccttccaccg gcgaagagct tacgcaaatt gcggaagctg atgccgcgga
      601 tgtggataag gcagtggccg cggctcgcgc ggcatttgag aagggccctt ggcggaagat
      661 gaccgcctcg cagcgcggca acctgatgtt caagcttgcg gatttgatcg aaaaaaatct
      721 ggacgagctt gcgcaactgg aagcgctgga taacggaaag ccttacagcg tggctcgcgc
      781 ggcggatttg cctttgacca tcgcttgtta ccgctactat gccggatggg ccgacaaact
      841 tcagggcaaa accattccag tgaatggcaa ctatttcacg tacaccaaac atgagcctgt
      901 cggcgtcgtg gctcagatta ttccgtggaa ttttccgctc ctgatgcagg cctggaagct
      961 gggtcctgcg ctggctaccg gctgcacggt ggtaatgaag cccgcggaac aaacacctct
     1021 gtctgcgttg cgcgttggcg aattggcgct ggaagctgga ttccctcccg gcgttttaaa
     1081 tttgttgcct ggctacggcc caaccgcggg agctgcattg gcgcggcata tggacgtgga
     1141 taaggtggcg ttcacaggct ccacggaagt gggtcatctg gttatgaaag ccgctgcgga
     1201 atcgaatttg aaacgcgtca cgctggaact gggcggcaag agtcccaaca ttatatttgc
     1261 ggacgccgac atggataaag ccgttgaagg gtcgcatttc gcgctgtttt tcaaccaggg
     1321 gcaatgttgc tgcgcgggtt cgcgtacttt cgtggaagac aaagtctatg acgagttcgt
     1381 cgagcgcagc gcagcgcgcg ccaagaagcg gacggtcggt aatccgttcg acaaaaatac
     1441 cgagcaaggc ccgcaggttg accaggaaca attcacaaaa gtgatgggct atatcgactc
     1501 cggcaagcaa caagccaaat tgatggctgg cggcaatcgc gtgggcgaca agggattttt
     1561 catcgagccc acggtgttct ctgatgtgaa ggacgacatg aagatcgctc aggaagaaat
     1621 ttttggcccg gtcatgtcca ttctgcgctt caaggatatt ggcgaagttg tggagcgcgc
     1681 gaacaaatcg ctctacggac tagccgccgc ggtttggacc aaggacatta ctaaagctca
     1741 cgccatcgcc gacaacgttc gtgcgggaac ggtttgggtg aattgctacg acgtgtttga
     1801 tgcagcagcg ccatttggcg gatttaagca atcgggcatc gggcgcgaac tcggcgaata
     1861 tggacttgcc aactacacgg aagtgaaaac cgtaaccatc aagctctaac cggtcgcggc
     1921 ataataaaag ggctgcaaat atctgaaatc ttgtcgtgct tccggcgaag acgtggttag
     1981 tgtgcgcaaa gccttcgacc gcgtcgtggc acgctattaa cgtgcggatc cgcgcctacc
     2041 agccgcatga ttttgacgcg ctctataagc ttgaccagtc ctgctttccg cggggcatag
     2101 cgtattcgaa aagcacgttg cgttattttt tgacactctc atccgcggag tgcttagttg
     2161 ctcaagaaaa ggacgatatt gccgggttca tctcgagcga gttggatgga ccgctcgcgc
     2221 acatcatcac gttggatgtc gcggaggcgc atcgcaggag cggcgtaggc tcgctgctgc
     2281 tagatgcggc ggagaataat gttttcgcgc agggtgttcg cactgtcttt ttggagacgg
     2341 tggtagataa tcacgcggct gtaaacttct ggctacgtca tggttacatc aaagaaggta
     2401 tcttgaagcg ctactatctg aaccgcatcg atggttacga aatgagaaaa acactttcat
     2461 cccggcatgg acaataaggt ggtggatgag ctgagagcgg aacgagtgtg cgcgccatgc
     2521 ggcaagcgaa gacaatcggg ggcgttgtgc ttatgcgccg aagtgcttcc acgttaaaag
     2581 acccatcctc caaaaacccg agggtggggc accctccgcg tcttttcaat tttctatcgg
     2641 ccaattactt ttcggcgttc ggtggaaagt atgagatgtc aactcgtcgt cgactttcaa
     2701 aataaaggtt tattaatcta gccacgattc ataaatgagg gcagaagtga aaaactgtgg
     2761 cagtgcaacg cctgcctttg tgtaagctaa gaggacgtcg gagggcactt gcacgattat
     2821 cttatgtcgg tggtgttggg aattgtcgaa gggctgacgg agtttttgcc ggtcagctcc
     2881 acagcgcatt tgcgaattgc tgaatatttg ctgaacatca gcttgcagag cggctattgg
     2941 aagatgtaca ccatcgtgat tcagattggc gcaatagcca ccttgccgat ttattttcgc
     3001 gagcacatcc ggcagttgtt ttccaccttc ccaaatggcg agcgaaacga tcgcacggta
     3061 ttcactcatc cgttgagcct ggtgatcatc gcgttcgtga ttaccgcggg gccttcttat
     3121 ctgctgacca aaattatcgg caaaaatctg gaaagtttgt acgtgatggg caccgcgctg
     3181 gtggttggcg gggtggtgat gtggctggtg gactggatga actcgaagtc ggaggcggcg
     3241 ggtgaagcgg ccggggcgaa cagccgcatc catacttggc gaatggataa catgagcctg
     3301 ggacaagcga tgtggatcgg cgcctgccag attttatcgg gggttttccc gggcacttcg
     3361 cgatcaatgt ccaccatcac tgcggggcaa gttgcgggca tgtcgcgatc ggcggcgctg
     3421 gaattttcct tttttctgtc tatgccgacc atggccatgg ccgtggctta ttccttattc
     3481 aaatcgctgc ggggaaaaga tgagaatccc atcggcgtgg cgcagatcga tgcgcatcaa
     3541 tggatcgtat tgctgatcgg gctggtggtt tcgtttgtgg tggcatacgg cgcggtggcg
     3601 tggttcatgg gctgggtgcg gaagcgtgga tttgcgccgt ttgcgg
//
LOCUS       DATCBB010000963         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_321744, whole genome shotgun sequence.
ACCESSION   DATCBB010000963 DATCBB010000000
VERSION     DATCBB010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321744"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VII37_10555"
     CDS             complement(<1..282)
                     /locus_tag="VII37_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070225.1"
                     /translation="MATRFPEGASQPLPVNIDWRTSPPVTRYYAVTPASPSRKSLALA
                     GFLFLLTLCTCLVAGAQFSVAFAHNESVSVDEFVRDFNLLYQHPAALLSG"
     gene            285..623
                     /locus_tag="VII37_10560"
     CDS             285..623
                     /locus_tag="VII37_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070226.1"
                     /translation="MIWRTLMAALGIGLVVELLLVRLYLWYLPGALAAPQNGQSNLWL
                     WEAIRLTQQPGLGLVQSWVGVVRPGYGELIGDMFLVPVIQWMVYAAVTYLILVMYLGK
                     RVSGATGRQD"
     gene            complement(691..984)
                     /locus_tag="VII37_10565"
     CDS             complement(691..984)
                     /locus_tag="VII37_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY5070227.1"
                     /translation="MIKLDIVNAVVTKTNISRTKAEQAVETVFESLKTALGRGQRIEL
                     RRFGVFSVKPRKTGIGRNPRTGEEVSIPPGKAVRFKPGKELQGLEGTKPPASA"
     gene            1146..1700
                     /gene="dcd"
                     /locus_tag="VII37_10570"
     CDS             1146..1700
                     /gene="dcd"
                     /locus_tag="VII37_10570"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY5070228.1"
                     /translation="MSVKPDRWIRRMALEHKMIEPFTDRQVREGVISYGVSSYGYDIR
                     VADEFRIFTNVNSTIVDPKNFDSRSLVDYKGDVCIIPPNSFALARSIEYFRIPRNVLT
                     ICLGKSTYARCGIIVNVTPFEPEWEGFVTLEISNTTPLPAKIYGNEGLCQVIFFESDE
                     DCEISYKDKKGKYQSQGGIVLPRL"
     gene            complement(1916..2722)
                     /locus_tag="VII37_10575"
     CDS             complement(1916..2722)
                     /locus_tag="VII37_10575"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY5070229.1"
                     /translation="MNSPLEFLVRHGYVLLLGWVFVEQLGLPIPSVPLLLAAGALAGA
                     GRMNIFVALFITMFAALCADSFWYQLGRIKGIAILQLLCKISLEPDSCVRRTEGVFSR
                     QGARSLVFAKFLPGVGAVAPPLAGVFHMRMARFMMFDSMGTLLWAGAYLGAGFIFSGQ
                     IEQIAAHLAYFGGHLGAVLLAAFILYIAYKFIARQRFIRQLRIARITVGELKEKLDAG
                     EEMVIVDLRHSMDFEADPQTIPGAFRMDAAELEEKNDRLPRDRDVILYCT"
     gene            complement(2751..3353)
                     /locus_tag="VII37_10580"
     CDS             complement(2751..3353)
                     /locus_tag="VII37_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY5070230.1"
                     /translation="MHKRAPTDFPVHDFIAERWSPRAFSDKPVAPDVLQSLFEAARWA
                     PSSSNEQPWGYIVATKDDAENFAKLVSVLVPSNVTWAQHAPVLALAVAELAFAKSGSP
                     NRNAQYDVGAASAWLTVEATSRGLFVHQMGGYDHDKARQVFQIPAGWEPIAAMAIGYP
                     GDPDSLPQPLRDREVAPRTRKPISQFVMSGSWKNKAAFLP"
     gene            complement(3398..4015)
                     /gene="wrbA"
                     /locus_tag="VII37_10585"
     CDS             complement(3398..4015)
                     /gene="wrbA"
                     /locus_tag="VII37_10585"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY5070231.1"
                     /translation="MSIKVQVIFYSMYGHVFKMAQAVSEGVKQVSGAEVSLYQVAELI
                     PDDILEKYGAKAAKASFANVTLASVDKLAEANAIIFGTPTRFGNMAAQMRNFLDQTGA
                     LWGKGALVGKVGSVFASTGTQHGGQETTITSFHSTLLHHGMIIVGVPYTESGLTYMDG
                     ISGGTPYGATTLAGSDGKRQPSENELKIARFQGKHVAEIATKLAR"
BASE COUNT          942 a         1045 c         1231 g          979 t
ORIGIN      
        1 gccgctcagc aatgctgccg ggtgttgata cagaagatta aaatctcgaa cgaattcgtc
       61 cacggagact gactcgttgt gcgcgaaggc caccgagaat tgcgcgccag caacgaggca
      121 ggtacacaag gtcaacagga acaggaaccc tgccagtgcc agacttttgc gcgaaggact
      181 agccggagtg acggcgtaat aacgggtcac cggcggactg gtgcgccaat ctatgttgac
      241 gggaagtggt tgactggcgc cttctgggaa tcgagtggcc acgaatgatt tggcgaacct
      301 tgatggcggc tctcggtata gggctggtcg tcgaactgct tctggtgagg ctatatttat
      361 ggtatttgcc aggcgcattg gcggccccgc aaaatggcca gtcaaattta tggctttggg
      421 aagcgatacg gctaacacag caacccgggc tgggcctggt tcagtcgtgg gttggagtcg
      481 tgcgaccggg gtatggggag ttgataggcg acatgtttct tgttcccgtt attcagtgga
      541 tggtttacgc cgcggtgacc tatttgattt tggtgatgta tctaggtaag agagtcagtg
      601 gggcaacggg acgccaagac tagttgctcg atcaggggct cgcatttaaa tttgttgaag
      661 caaaatgtcg aattgaaaaa tgcagtttga tcaagctgac gcagggggtt tggtgccctc
      721 gaggccctgc aattccttgc cgggtttgaa gcgcacagct tttcctggcg gaatgctgac
      781 ttcttcgccg gtgcgcggat tgcgaccgat gccggtctta cgaggtttca cgctgaagac
      841 gccaaagcgg cgcaattcga tgcgctgccc gcggcccaag gcggtcttga gagattcgaa
      901 caccgtctcg acggcctgct cggctttagt gcgactgata ttagttttag tcaccacggc
      961 gttaacgatg tccaacttaa tcaaaaggaa cctcccggga gcgtgggcag atgctagagg
     1021 gtgcggctca ggttgtcaag atgcggaggc gtacactaca ctgacgttcc tttgcgacga
     1081 gcggttcaat ttttttgcag gaatgaaggc aaatttctat cgtgaacttt tccaggagga
     1141 agtgaatgtc ggttaagcca gatcgctgga tccggcgcat ggcgctggag cataaaatga
     1201 ttgaaccttt tacggaccgc caagtgcggg aaggagtcat cagttacggg gtctcgtcgt
     1261 acgggtatga catccgggtt gccgatgagt ttcgtatttt tacgaatgtt aattccacta
     1321 ttgttgatcc gaaaaatttc gattcgcggt cgctggtgga ctacaaaggc gatgtctgca
     1381 ttatcccgcc taattcgttt gcgctggcgc gttcaattga atattttcgg attccgcgaa
     1441 atgtgttgac gatttgttta ggaaaatcga cttacgcgcg gtgtggaatt attgtgaacg
     1501 tgacgccatt tgagccggag tgggaagggt ttgtgacttt ggagatcagc aacaccacgc
     1561 ccttgcccgc gaagatttat ggaaatgaag ggctgtgtca ggtgattttc tttgagtccg
     1621 acgaggattg cgagatcagc tataaagata aaaagggaaa gtatcagtcg cagggtggga
     1681 ttgtgttgcc acgtctttag cactcggagc cggtcgaaaa ctggcgcagc tcgaaacttc
     1741 gtgagattta tttcagcgcg ggttcgatag ggataagttt tacggttgga gctgtgccct
     1801 ccaatgggta gccttgttca cgccaggctg cgagaccgcc ttgcaatggc ctgatgcgct
     1861 tgattccttg cttgcgtagg agcagcgcca cacgggcgct ggtggcttcg ttcggtcaag
     1921 tacagtacaa gatgacgtcg cgatcacggg gcaagcgatc gtttttttcc tctagctctg
     1981 ccgcatccat acgaaatgcg ccgggaatag tttgtggatc ggcttcgaag tccatggagt
     2041 ggcgcagatc cacgatgacc atttcttcgc ctgcatcgag cttttctttt aactcgccga
     2101 ccgtgatgcg cgcgatgcga agctggcgga tgaatctttg gcgagcaata aatttgtagg
     2161 cgatgtatag gatgaatgct gccagaagca ccgcgcctaa gtgaccgccg aaataagcaa
     2221 gatgcgcggc gatttgttcg atctgtccac tgaatataaa tcctgcgccc aagtatgcgc
     2281 cagcccacag cagggtgccc atcgaatcga acatcataaa acgcgccatg cgcatgtgga
     2341 agacgccagc caacggcggc gctactgctc cgacacccgg aagaaattta gcgaagacaa
     2401 gcgagcgggc gccctgccga gagaaaacgc cttccgtgcg tcttacgcag gagtccggct
     2461 ccagcgaaat tttgcagagc aactgcagga tggcgatgcc tttaattcgg cctaattgat
     2521 accagaaaga atccgcgcag agggccgcga acatggtgat gaagagcgcc acaaaaatgt
     2581 tcatccggcc cgcgcccgcc aaggcgccgg cggccagaag caatggaacc gagggaatgg
     2641 gcaggccaag ctgttcaacg aatacccagc caagcagaag tacgtatccg tgacggacca
     2701 ggaattcgag agggctgttc atctgaggcc gttaaaaatt taccctaaat tcaggggagg
     2761 aacgccgctt tatttttcca gcttccggac atgacaaact gtgagatggg ttttcgagtg
     2821 cgtggcgcga cttcgcgatc gcgcaaaggt tgcggcagag aatccggatc tcccgggtag
     2881 ccaatggcca tggcggcgat aggttcccag cctgcgggta tctggaaaac ttgtcgcgct
     2941 ttgtcatggt cgtaaccgcc catttggtgg acgaacagtc cgcgcgaagt ggcttcgacg
     3001 gtgagccagg cgctggcggc gccaacatcg tattgcgcgt tgcgatttgg ggatccgctc
     3061 ttcgcgaaag ccaattcagc cacggcgagg gccaagaccg gcgcgtgctg ggcccacgtt
     3121 acattggacg gcaccaaaac gctgactagc ttggcgaaat tttccgcatc gtccttggtt
     3181 gcgacgatat atccccacgg ttgttcgttg ctgctagagg gcgcccagcg cgctgcttca
     3241 aataaactct gcagcacatc cggcgcgacg ggcttatcgc tgaaggcccg cggactccag
     3301 cgctcagcga taaaatcgtg taccggaaaa tccgttggcg ctctcttgtg catgaacgtt
     3361 cctttcggtg tttagagcag cggggcttag tagaaattta gcgagctagc ttggtggcaa
     3421 tttcggcgac atgcttgccc tggaagcgcg caatcttcag ttcattttca ctgggttggc
     3481 gcttaccgtc gctgcccgcc aatgtagtgg ctccgtaagg agtgccgcca gagatgccgt
     3541 ccatgtaggt cagcccggat tcggtatagg gcacaccgac gataatcatg ccgtggtgca
     3601 acagcgtgga atgaaaactc gtaatggtgg tttcctggcc gccgtgctgc gtgccggtcg
     3661 aagcaaacac gctaccaact tttccgacca acgcaccctt gccccacaaa gcgccggtct
     3721 ggtcgaggaa attgcgcatt tgcgcagcca tatttccgaa acgcgtcggc gtgccgaaga
     3781 taatcgcgtt cgcttcggca agcttgtcta ccgatgcaag cgtgacgtta gcgaagctgg
     3841 ctttggcggc cttcgcgccg tatttttcca aaatgtcgtc ggggataagc tctgcgacct
     3901 ggtacaacga gacttctgct cctgaaacct gtttaacacc ttcggagacg gcctgcgcca
     3961 ttttgaaaac gtggccgtac atgctgtaga aaatcacctg aactttaatg ctcacgatgt
     4021 gcacctttcc tttcgacgtt cgacgggcaa tgttgggaaa gccttacaga aaatccggtc
     4081 ccaaaacaga gacgtaagct gcaagtaacc tattagcgtg ggatcacagc gtcagcgtgc
     4141 atccttttca tttcgaccgc gccaaattat ttcgggactt gcttgatcat ttcggca
//
LOCUS       DATCBB010000964         1969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_127590, whole genome shotgun sequence.
ACCESSION   DATCBB010000964 DATCBB010000000
VERSION     DATCBB010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127590"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VII37_10590"
     CDS             complement(<1..974)
                     /locus_tag="VII37_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070232.1"
                     /translation="MNPLSSNLKTFWRRLSGFDIVALVIFVLGAVATTAELSGSIANF
                     VKFLAVLCLIYLLFRFIGWWRSRLLWSLRNRIIVAYLFIALVPILLIVTLVGLAGRIL
                     YSQLGAYLLYEDIHTRINTIADMAEHIAAAHGPATRGSSQEELESILAAQSKAVHDRE
                     LPELSITFSDDATLLHRVAKGADAFAGLIQEEDKLSLMSLRAIHGKDHVRVVQLRVPV
                     TPELLSAIAPDVGAIQLNPMRKISAADKETAIYQSAGDKYAAGKPINARARVLQPQGF
                     WIDPAVGVVSRLNAVYVSRGGTVEYDRPVLAVTNARPSRLSARIFNSLG"
     gene            complement(1144..>1969)
                     /locus_tag="VII37_10595"
     CDS             complement(1144..>1969)
                     /locus_tag="VII37_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5070233.1"
                     /translation="SRYVSTANWGMDWHSFAPMGFSGIGAGAAYIFFAYIGFDAVSTT
                     AQEAKNPQRDLPIGIIASLLVCTFLYIAVAGVLTGMVHWQDINIEAPVARAFMDRGLT
                     TASHIITLGALAGLTSVMLVMLLGQTRVLYSMANDGLLPKKFFAAVHPKYRTPYKNTI
                     LVGLLAAIVGSITPIDDIGRMVNIGTLLAFVIVCIAVMVLRHTNPGQARPFRTPWVPV
                     VPVLGILFNGYMMYKLGWVNWARLIVWLVIGLVIYFTYSRHHSRVSNPGRVEVPNP"
BASE COUNT          505 a          639 c          435 g          390 t
ORIGIN      
        1 ccaagcgaat taaatatccg cgcgctcaag cgcgacggcc gtgcattggt caccgccaaa
       61 accggccggt catattccac cgtgccgcca cgacttacat aaaccgcatt gagcctcgac
      121 accaccccga ccgcgggatc aatccagaaa ccttgcggtt gcaacactcg cgccctagcg
      181 tttatgggct tgccagccgc atacttatcg cctgccgact ggtaaatcgc ggtctcttta
      241 tctgcagctg aaattttccg catcggattt aattgaatcg cgcccacgtc cggcgcaatg
      301 gcggaaagca gttcgggcgt aaccggaacg cgcaactgca ctactctgac gtggtccttt
      361 ccatgaatcg cgcggaggct catcagcgac agtttgtctt cttcctgtat taagcccgcg
      421 aaggcgtccg ctcccttcgc cactctatgc agtagcgtcg catcatccga aaaggtgatc
      481 gacagttcgg gcagttcgcg gtcgtgcacc gctttcgatt gcgctgccag aatgctctct
      541 aactcttctt ggctgcttcc ccgcgtcgca ggtccgtgcg ccgccgcaat gtgctcagcc
      601 atatccgcga tggtattaat gcgcgtatgg atatcttcgt aaagtaggta agcccccagt
      661 tgcgaataca aaatccgtcc cgcaagccca accaacgtaa caatcaacaa aatcggcacc
      721 agcgcgataa acaggtaagc cacaatgatc ctgttgcgca aactccacaa cagccgcgac
      781 cgccaccacc cgataaatcg aaacagtaga taaatcaggc atagaaccgc caaaaatttt
      841 acaaaattgg cgatactccc actgagttct gcagtggtag ccactgcgcc caaaacaaaa
      901 ataaccagcg caacaatgtc aaagcccgat agccggcgcc aaaacgtttt caaattggaa
      961 gagagaggat tcatcggcaa gggtagactc aaacacttta cctgactttc ccgcgctatg
     1021 agaatgcact aacgcccgta tatgcattcc accgcacgtc caccaggcac aaacgcagga
     1081 agccttctcc acctgatttt attcaaggga gaattggacc tgacaacaga tcaattcacg
     1141 tttctaggga ttaggaactt cgactcgccc cggattactc acccggctat ggtgccggct
     1201 atacgtaaaa taaatgacca gcccgatgac cagccacaca atcagccgcg cccagttcac
     1261 ccaccccagc ttgtacatca tgtagccatt aaaaagaatc cccaatacgg gcacaaccgg
     1321 cacccacggc gtgcgaaacg gtcgcgcctg tcccggattt gtatggcgca aaaccatcac
     1381 cgcaatgcac acaattacaa acgccagcag cgtcccgata ttcaccattc ttccaatatc
     1441 gtcgatcggc gtgatgctcc ccacgatcgc cgccagcaat ccaacaagaa tcgtattctt
     1501 gtaaggggtt ctgtacttcg gatgcaccgc cgcgaaaaac ttcttcggca gcagcccgtc
     1561 gttcgccatc gagtacagca cccgggtttg ccccagcagc atcaccaaca tcacgctcgt
     1621 caatcccgcc agcgccccca acgtaataat gtgcgacgcc gtggtcagtc cccggtccat
     1681 aaacgcccgc gccaccggag cctcaatatt gatgtcctgc caatgcacca tcccggtcag
     1741 caccccggcc accgctatat aaaggaacgt gcataccaac aacgaagcaa taatcccgat
     1801 cggcaaatcc cgctgcgggt tctttgcttc ctgcgccgtc gtggacaccg cgtcaaatcc
     1861 gatgtacgca aaaaatatgt acgccgcccc cgctccaatg ccgctgaatc ccatcggcgc
     1921 aaaactgtgc cagtccattc cccaattcgc cgtggacaca taacgcgag
//
LOCUS       DATCBB010000965         1688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_450741, whole genome shotgun sequence.
ACCESSION   DATCBB010000965 DATCBB010000000
VERSION     DATCBB010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450741"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VII37_10600"
     CDS             complement(<1..1661)
                     /locus_tag="VII37_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY5070234.1"
                     /translation="MNVIVTKVPLLINFVDSTNNAILEDQPTLPMGWDGDSVHIWKRM
                     PADENYFGLGDKAGRMNRRNRSFTMWNTDEFGWQESSDPLYKTIPFFIGLRNGAAYGV
                     FFDNTYRSSFDFGKESADYFSFGAAGGELNYYFFSGPDPKKIISDYTALVGRAELPPL
                     WSLGFQQSRYSYYPEARAREIVATYRQKKIPLDAIYFDIDYQQGYAPFTVNRQYFPTF
                     AKMISDFRSQGVYTVLITDLHIKKDPNPAYTPYATGMQRDVFVKNPDGSVFVGPVWPG
                     PSVFPDFTLTNAREWWGEQYKDFVSMGVAGFWNDMNEPSVFERADKTMPLDVKHRLDD
                     GTTRDHRAIHNVYGMENVRATYDGLRKLQPDERPFVLTRAAYSGTQRYAATWTGDNSS
                     TWNHLSMSTPMLLSMGICGYSLVGDDIGGFAGTPPADLLTRWIELGAFNPIYRDHTGK
                     GTGDQEPWVHGPEHEMIRRHYIELRYQLLPYIYTGIEEMTRTGLPFMRPLFVEYPQSK
                     GIEGDDHDFLFGRDLFVAPVDREMLDAKDFHLPPGDWYDFWTSTKL"
BASE COUNT          391 a          497 c          411 g          389 t
ORIGIN      
        1 agtttagtgc tcgtccaaaa atcgtaccaa tcgccgggcg gcagatgaaa atctttggca
       61 tccaacattt ctctatccac cggagccaca aacagatcac gcccaaacag aaaatcgtga
      121 tcgtcgccct ctatgccctt cgattgcgga tactccacaa acaacggccg catgaaaggc
      181 agtccagttc gcgtcatctc ttcaatgccg gtgtaaatat acggcagcaa ctggtaacgc
      241 aattcgatgt agtggcggcg aatcatttcg tgctccggcc cgtgcaccca cggttcctgg
      301 tcgccggtac ctttcccggt gtggtcgcga taaatgggat tgaacgctcc cagttcgatc
      361 caacgcgtca acaaatcggc cggcggcgtg cctgcgaatc cgccgatgtc gtcgcctacc
      421 agcgagtacc cgcaaattcc catactcaaa agcattggcg tgctcatcga caagtgattc
      481 caggtgctgc tgttgtctcc cgtccacgtc gctgcatacc gctgcgttcc cgaatacgcc
      541 gcacgcgtca gcacaaaagg ccgctcatca ggctgcaatt tacgcaaccc atcataagtg
      601 gcgcgcacgt tttccattcc atacacgtta tgaatcgcgc ggtggtctcg tgttgttccg
      661 tcatccaggc gatgcttcac gtccagtggc atggtcttat ccgcgcgctc aaacacggac
      721 ggctcgttca tatcgttcca gaagcccgcc acacccatgc tcacaaaatc tttgtattgt
      781 tcgccccacc actccctggc gttcgtgagc gtaaaatccg gaaacacgct gggaccgggc
      841 cacaccggac cgacgaaaac gctgccatca ggatttttaa cgaagacgtc ccgctgcatt
      901 ccagttgcgt acggcgtgta agccgggttc ggatcttttt tgatgtgcag atcggtaatc
      961 agcacggtgt acacgccctg cgagcgaaaa tccgaaatca tttttgcaaa cgttggaaag
     1021 tattgccggt ttaccgtgaa cggtgcgtat ccctgctggt aatcaatatc gaaataaatg
     1081 gcgtccagcg ggattttctt ttgccggtag gtcgccacaa tctcgcgcgc tcgtgcttcc
     1141 ggatagtagc tgtagcggct ttgctggaac cctaagctcc agagtggagg taactcagcg
     1201 cgtcccacca gcgcggtgta atcagaaata atcttcttcg gatccggccc ggaaaaaaag
     1261 taataattca gctcgccgcc cgccgctcca aacgaaaagt aatctgccga ctcttttcca
     1321 aaatcgaaac tgctgcgata agtgttatcg aaaaacactc cgtacgccgc gccgttgcgc
     1381 agcccgataa aaaatggaat ggtcttgtat agagggtcgc tggactcctg ccaaccaaac
     1441 tcatcggtgt tccacatggt aaaagagcgg ttccggcgat tcatgcggcc agccttgtcg
     1501 cccagcccaa agtaattttc atccgcaggc atgcgcttcc agatgtgcac gctgtcgcca
     1561 tcccatccca taggtagcgt cggttggtcc tcgagaatcg cgttgttagt tgaatccaca
     1621 aaattaatga gtagcggcac cttcgtcaca atcacattca cggcgcccgc gttgatgcgg
     1681 acttcttt
//
LOCUS       DATCBB010000966         1096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_191802, whole genome shotgun sequence.
ACCESSION   DATCBB010000966 DATCBB010000000
VERSION     DATCBB010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191802"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VII37_10605"
     CDS             <1..491
                     /locus_tag="VII37_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5070235.1"
                     /translation="EIRAYIAALAQTIPSADVHGLRQFTDAEARIYNRITGLLTAITA
                     LVLVLTTLCVMAAMTNVAMERRNDVGLMKAIGGATRRVLRLFLAEAALLGLTGGIVGA
                     GAGILLSIWLGKTVFGVAARPRLIVYPVSVAITILVAIVSAYPLRRLASISPASVFRG
                     EA"
     gene            488..>1096
                     /locus_tag="VII37_10610"
     CDS             488..>1096
                     /locus_tag="VII37_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5070236.1"
                     /translation="MSPLDSAAQKAPQVIVESLVKQYGALRALDGVSFSVEPGEWVAL
                     MGPSGSGKTTLINILGGLDTLTSGRVIVDGVHLSKHTENQLVRYRSEKVGFVFQQFHL
                     VPYLSALENVMLAQYFHSSTDEKQAEEALHRVGMGDRLTHLPAQLSGGEQQRVAIARA
                     LINQPKLILADEPTGNLDSNSSTEMLAFLRRSVTEFDQSIVMV"
BASE COUNT          203 a          330 c          307 g          256 t
ORIGIN      
        1 gtgagattcg cgcttacatt gccgcgctgg cgcaaacaat tccgtccgcg gatgtccacg
       61 gcttgcgcca gttcaccgac gccgaggcca ggatttacaa tcgcatcacc ggattactga
      121 ccgccataac cgcgctggtc ctggttctca ccactctgtg cgttatggcc gccatgacca
      181 acgtggccat ggagcgcaga aacgatgtcg gcttgatgaa ggccatcggc ggtgccacac
      241 gtcgcgttct gcgtttattt ctagcggagg ccgcgctgct tggcctcaca ggcggaatcg
      301 tgggagccgg tgcaggaatt ttgctttcca tctggctggg gaaaaccgtt ttcggtgtgg
      361 cagcgcgccc tcggttgatt gtgtatcctg tttccgtggc tatcaccatt cttgtcgcta
      421 ttgtcagcgc ctatccgctt cgacgtctcg ccagtatttc gcccgcttcg gtatttcggg
      481 gggaagcgtg agtccgctcg attccgccgc tcagaaggcg cctcaggtaa ttgtagagtc
      541 tttggtgaag cagtacgggg cgttgcgcgc cctcgatggc gtcagcttca gtgtggagcc
      601 cggagaatgg gtggcgctca tgggcccgtc cggctccggc aaaaccactt tgatcaatat
      661 tcttggcggg ctggacacgc tcacctccgg gcgcgtgatt gtcgacggcg tccatctctc
      721 caaacacact gaaaatcaac tggtgcgcta ccgttccgaa aaagttggct tcgtattcca
      781 gcaattccat ctcgttccgt atctttctgc gcttgaaaac gtgatgctgg cgcaatattt
      841 tcacagcagc accgatgaaa aacaggccga agaggcgctg catcgcgtcg gcatgggcga
      901 tcgactcact catcttcccg cgcagctgtc cggcggtgaa cagcagcgcg tagccatcgc
      961 ccgcgcgctg atcaatcagc caaaattaat tctcgccgat gagcccaccg gaaatctcga
     1021 ctcgaactcc tccaccgaga tgcttgcctt cctacgccgt tcggtgaccg agttcgacca
     1081 gtcgatcgtc atggtc
//
LOCUS       DATCBB010000967         2955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_450800, whole genome shotgun sequence.
ACCESSION   DATCBB010000967 DATCBB010000000
VERSION     DATCBB010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450800"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VII37_10615"
     CDS             complement(<1..682)
                     /locus_tag="VII37_10615"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5070237.1"
                     /translation="MATTDYTNVTAALGGGDEEREREHATRLAQRYRCGFVDLREQRI
                     DPELFRTIPADLMFRYNFVPLEVHNNLLSIAVADPSQVLLSDELPLLLGKKLAIKVAT
                     AHQISDLLKRTEQSQRVLEQATEAFTLQVSKEEGESDETISGDRLTRDTAVSPVVRLV
                     ETVIFTALERRASDIHIEARDSEVAVKYRIDGVLQHAMQPIAKEWHSTVISRIKVLSD
                     LDIAERRVP"
     gene            complement(682..1893)
                     /locus_tag="VII37_10620"
     CDS             complement(682..1893)
                     /locus_tag="VII37_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5070238.1"
                     /translation="MGEFVCRVADANGRVFSQVEAAGTTDEARQKLVDRGFYVYSVES
                     RGSVLGMLSRRKQRQIGGSDFLILNQQFNTLIKAGIPILRALDLLATRATTPKLRPVI
                     TQIRDRVREGKSLSEAVDEAGVFSKVYSTAILAGEKSGNLAGVLDFYIAYQKVSTGVR
                     KKILASLVYPVLLVVVATVIVTYLVTAVVPKFAVLYRDLGVDLPSSTKLLMALTVDYR
                     FVVLGVMGALALLAFGVFLWSRTDEGGATFDRLKFKLPIIGDTLLKFQVAQFSRTLAT
                     LLTGGTTLVTGLQTASDALTSKLLRLTILRATQRVREGESLHASLASTGVMPEMALDM
                     VEVGESSGALGPMLTSIAEFYEEEVSLRLGALVSIIEPVMLIFMGILVAFILISLYLP
                     IFSLSVSGGAK"
     gene            complement(1996..2748)
                     /locus_tag="VII37_10625"
     CDS             complement(1996..2748)
                     /locus_tag="VII37_10625"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY5070239.1"
                     /translation="MMTGALVSSTLTASAAQKSADTEPIRRMEHRYKAAKTLQATFLE
                     RYTENDRTVRVESGVAYFRRPGKMRWEYAAPESNLFLIDGKMAWFYVPADHTVTRVPA
                     KQSTDWRTPLALLAGETKVSRVCARVELVPDQLPERPGDAVFSCTLRGSADTKLRSGN
                     KNAESGEASGLTGHAPQGSDQEDTVLLEIGRESGELVRIVVRNKGGVGIEFHFANWVD
                     NPPIAEALFRFEPPKGVAVVNAEFPAGTSNSE"
BASE COUNT          660 a          855 c          803 g          637 t
ORIGIN      
        1 gggggacgcg ccgctcagcg atgtccagat cgctcaaaac cttgatgcgc gaaattaccg
       61 tggagtgcca ttctttggcg atgggctgca tggcgtgctg taaaacgccg tcgatacggt
      121 acttcacggc gacttctgaa tcgcgtgctt caatgtgaat gtcgcttgct ctacgctcca
      181 gagcggtgaa aattaccgtt tccaccagcc gaaccaccgg gcttaccgcg gtgtcgcggg
      241 tcagccggtc gccggaaata gtttcgtcgc tctcgccttc ttcctttgaa acctgaagtg
      301 tgaacgcttc ggtagcttgt tccagcacac gctgagactg ctcggtgcgt ttcagcaggt
      361 cgctgatctg atgagcggtg gccaccttga tggccagctt tttccccaga agcagaggca
      421 actcgtcgct cagcagcacc tggctgggat cggccaccgc gatggacaac aaattgttgt
      481 gaacttccag tggcacgaag ttatagcgga acatcaagtc cgccggaatg gtgcggaaaa
      541 gttccgggtc gatacgctgc tcgcgaagat cgacaaagcc gcagcggtag cgctgcgcta
      601 atctagtcgc gtgctcgcgc tcgcgttctt cgtcaccgcc acccaatgcc gctgttacgt
      661 ttgtgtagtc tgtagtcgcc attacttagc tccgcctgaa accgatagcg agaatatagg
      721 cagataaagt gaaatgagta tgaacgccac caaaattccc atgaagatca acataaccgg
      781 ctcaataatg gataccagtg ccccgagccg tagactaact tcttcctcat aaaattccgc
      841 gatgctggtg agcatgggtc ccagcgcacc gctgctttct ccgacttcca ccatatccag
      901 cgccatttct ggcattacgc cggtggacgc cagagaggcg tgcagcgatt cgccttcgcg
      961 aactctctgc gtggcgcgaa gaatcgtgag tcgcaataat ttactggtca gcgcatccga
     1021 ggcggtttgc agtccggtta ctaaagtggt gccgcccgtc aagagtgttg ccagtgtgcg
     1081 tgaaaattgc gcgacctgaa acttcaacaa ggtgtcccca atgatcggca gcttgaactt
     1141 gagacgatca aaagtggcac cgccttcatc agttcgcgac caaaggaaaa ctccgaaagc
     1201 caggagcgca agtgctccca taactcccag taccacgaag cgataatcaa ctgtcaaggc
     1261 catcaaaagt tttgtcgaag agggcaggtc cacgcccaag tcccgataca gaacagcaaa
     1321 tttgggaacc actgcggtga ccaggtacgt gacgatgacg gtagcaacca ccaccaaaag
     1381 taccgggtac acgagggagg ccaggatctt ctttcgaaca ccggtactta ctttttgata
     1441 cgcaatgtaa aagtccagca cgccggcaag atttccgctc ttctcgcctg ccagaatcgc
     1501 ggtggaataa acctttgaga aaactccagc ctcgtcgacg gcttctgaca gagactttcc
     1561 ttcccgtacg cgatcgcgaa tctgggtgat cactgggcga agcttcggag ttgtggcgcg
     1621 ggtggccagc aggtctagcg cacgaagaat cgggatgccg gccttaatga gtgtattgaa
     1681 ttgttgattc aggatgagaa aatcggagcc gccgatttgc cgctgtttgc ggcgactcag
     1741 catcccgagc acacttccgc ggctctctac ggagtaaacg taaaaacccc ggtccaccag
     1801 tttctggcgg gcttcatccg tggtgccggc ggcctcgact tgcgaaaaca cccggccgtt
     1861 ggcatccgcg acgcggcaaa caaattcccc catataccct cagtttaccc caagtaagat
     1921 gtctgcgcgg acaaccggga tggttgcccg ttccttccca gaaaggggta ccccatggcg
     1981 cagggtgacc cgaaactact ccgaatttga cgttccggcc gggaattccg cgttcacgac
     2041 ggccactccc ttgggaggtt cgaagcgaaa caaggcttcc gcgatgggag gattatccac
     2101 ccaattggcg aaatgaaact caattccgac gccgcctttg ttgcgcacca caatgcgaac
     2161 caactcacca gattcgcgcc caatttccag cagcacagta tcttcctgat cgctaccttg
     2221 cggtgcgtgc ccggttagcc cggacgcctc gccggattcg gcgttcttgt tgccgcttcg
     2281 tagtttcgtg tcggcggatc cgcgcaaggt gcaactgaaa accgcatcac caggtctttc
     2341 ggggagctgg tcaggcacca gttcaacccg cgcgcatacg cgggatactt tcgtttcgcc
     2401 tgccaacagc gctaacggag tccgccagtc cgtgctttgt ttggcgggta cgcgcgtcac
     2461 ggtgtgatcg gcgggcacgt aaaaccacgc cattttgcca tcgatcagaa acaaattact
     2521 ttctggagct gcatattccc aacgcatttt gccgggtcgc cgaaaatagg ccacgcctga
     2581 ctctacccgc acagtccggt cgttctcggt gtagcgctcc agaaaggtcg cctgcaaggt
     2641 tttggcggcc ttataacgat gttccatgcg gcgaattggc tcggtatcgg cggacttctg
     2701 tgccgcgctt gcggttaaag tggaactgac cagggcgccc gtcatcattg ttatcttgag
     2761 agcagacgca cagattctcg cggcgcctac caaatactct ctgacatcca cccgaccagt
     2821 ataacgaacg acggcagcat ccgtctggcg acgatcggtg tcgtggagac gggtgcggcc
     2881 attagaacgt gacgcgccgt gcagttatgg aactgcgcac ctgagaatca ccctattcag
     2941 gtaggcgcgt gccaa
//
LOCUS       DATCBB010000968         1493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_321930, whole genome shotgun sequence.
ACCESSION   DATCBB010000968 DATCBB010000000
VERSION     DATCBB010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321930"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VII37_10630"
     CDS             complement(<1..1211)
                     /locus_tag="VII37_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5070240.1"
                     /translation="MYVELHSRSAFSFLEGSSIPEELIGVCANLGMPAMGLLDRDGLY
                     GSPRFHLAAKKIGIKAHIGVEVTCAPFSSRHHSGFRLPLLVASRAGYNNLCRLITKMK
                     LRVGRKEGALSSESDFIEHAGGLICLTGGDEGPLAAALAQGGPQAAFEAVKRLTELFG
                     TPNVFVELQRHFQRAEEYRNRIAIEIGEALHLPLLATNGVNYAAPHERELADAFTALR
                     HHRTLATAGRLLAHNAERYIKSPAAMRALFADLPKAITNTVELSSRLEFTLNNLGYEF
                     PRFPVPEGESMMSFLRERTREGWQTRYGRANAELKKRAQRQIERELNLIERLNLAGYF
                     LIVWDLVRFCRTEKILVQGRGSAANSAVCYSLGITAVDPISMELLFERFLSDERGEWP
                     DIDLDLPSGADR"
     gene            complement(1241..>1493)
                     /locus_tag="VII37_10635"
     CDS             complement(1241..>1493)
                     /locus_tag="VII37_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070241.1"
                     /translation="PLNASVTLREAKPVRLRCLQREEIAGDIVWTAGPWRSSGDWSEQ
                     DGWSREEWDIAVTAETGLVLYRLVQDKFSGKWFLEGTYD"
BASE COUNT          340 a          447 c          389 g          317 t
ORIGIN      
        1 cggtcagcgc cactcggaag atcgagatcg atgtcgggcc actccccgcg ctcgtcggaa
       61 agaaatcgtt caaacaataa ttccatgctg atcggatcga cggcggtaat gcccagcgaa
      121 tagcaaaccg cgctattggc ggccgatccg cggccctgca ccagaatctt ttctgtgcga
      181 cagaatcgca ccagatccca gacgatcaaa aagtatccgg caagattcag cctttcaatc
      241 aagttcaact cgcgctcgat ctgccgttgc gcccgctttt tgagttcggc atttgcgcgg
      301 ccatatcgtg tctgccatcc ctctcgcgtg cgctcgcgta aaaacgacat catgctttca
      361 ccctcgggca cgggaaaccg tgggaactca tatcctagat tgttcagcgt gaattcaagc
      421 cgcgaggaaa gttcaactgt gttggtaatg gccttcggta aatcagcaaa gagggcccgc
      481 atcgcggcag gcgatttgat gtagcgctca gcattgtgcg ccagcaggcg cccggcagta
      541 gccagcgtgc gatgatgccg cagcgcggtg aatgcatccg ccagttcacg ctcatgaggc
      601 gcggcgtagt tcacgccatt ggtggcgagc agcggcaggt gcaatgcttc gccaatttcg
      661 atggcaatcc gatttcgata ttcttcggcg cgctgaaaat gacgttgcaa ttcgacgaaa
      721 acatttggcg tcccaaacag ttctgttagc cgctttaccg cttcaaatgc cgcttgcggc
      781 ccgccttgcg ccagggcagc tgcgagaggc ccttcgtctc cgccagtcaa acaaatcagg
      841 ccgccggcgt gttcaataaa atccgactcc gaagatagcg ctccttcttt gcgtccaacg
      901 cgcagcttca tcttcgtaat caggcggcag agattgttgt atccagcgcg cgaagcaacc
      961 agcagtggca gtcgaaagcc ggagtgatgc cgtgaagaga acggagcaca cgtcacttcc
     1021 acgccgatgt gtgccttgat ccctattttt tttgcggcga gatgaaagcg cggcgaccca
     1081 tataggccat cgcgatcaag caaccccatc gcaggcattc caagattcgc gcacactcca
     1141 atcaactcct cgggaatcga agagccttca aggaagctga atgcggaacg ggagtgaagt
     1201 tcaacgtaca tcgttcttta actacgatct ttaactatga tcagtcatac gttccttcca
     1261 aaaaccattt cccactgaac ttgtcctgca ctaagcgata caacacgagt ccagtttcgg
     1321 cggtcacggc gatgtcccat tcctcgcgcg accagccatc ctgttccgac caatcgcctg
     1381 aagagcgcca tgggccagcg gtccagacga tgtcacccgc gatctcctcg cgctgcaggc
     1441 agcgcaatcg caccggcttc gcttcgcgca gagtaacgct tgcattgagc ggc
//
LOCUS       DATCBB010000969         1089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_450850, whole genome shotgun sequence.
ACCESSION   DATCBB010000969 DATCBB010000000
VERSION     DATCBB010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450850"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..771
                     /locus_tag="VII37_10640"
     CDS             37..771
                     /locus_tag="VII37_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEY5070242.1"
                     /translation="MPTARLTDLTVRALPMGTHFDATTPAFGIRVGKHRRTWIVMRGE
                     ERRRIRIGHYPAVTLSEARKEAKRLLAATNLSGKRVTLGEAYETFKTDYIARRKERTQ
                     RDYKRMIEKHFLPTLKSKRMDAITSYMLSAITDGLADTPSEQAHAQVVCRIFFRWAIR
                     PQRRYISINPLEGMQIAKSKKRKRILTDGELRAVWIAAGQIEGDFGEIVRLLILMGQR
                     RGETGALCNLTRYRDGQTPKPTHASS"
     gene            848..>1089
                     /locus_tag="VII37_10645"
     CDS             848..>1089
                     /locus_tag="VII37_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3768 domain-containing protein"
                     /protein_id="HEY5070243.1"
                     /translation="MNESSVARIQELNDAFRKTFAGGKMVMTANVAALPEMVKASALV
                     KLAEFNDFTPENDPHGEHDFLSFEHCSRTFFWKCDY"
BASE COUNT          264 a          320 c          293 g          212 t
ORIGIN      
        1 cagcacacac gatttgagag ccaaaaagag ccaaaaatgc ctaccgcacg tctcaccgat
       61 ttgacggttc gcgctcttcc aatgggcacg cactttgacg ccactacccc ggcgtttggc
      121 atccgcgttg gcaagcatcg ccgcacgtgg attgtcatgc gaggcgagga acggcgccgt
      181 atccggatcg gccactatcc ggctgtcact ctttctgagg cccgcaaaga ggcgaagcgt
      241 ctccttgccg caacgaacct cagcggcaag cgggttacgc ttggagaggc ctacgaaacc
      301 ttcaaaaccg actacatcgc ccgcaggaag gagcgcaccc aaagagacta caaacggatg
      361 atcgaaaagc acttcctccc tacgctcaaa tccaagcgta tggacgcgat cacctcctac
      421 atgctgtcag ccattacgga cggcttggcc gacaccccca gcgaacaggc ccatgcccag
      481 gttgtgtgtc ggatattctt ccgttgggcc attcggccgc agcggcgata catttccatc
      541 aatccgcttg agggcatgca gatcgccaaa tcgaagaagc gcaagcgcat tttaacggac
      601 ggcgagctcc gcgcggtttg gattgcagcc ggacagattg agggcgactt cggcgaaatc
      661 gtccggctgc tcattcttat gggccagcgc cgcggcgaaa ccggtgcgct ctgtaacctc
      721 actcgctatc gagatggcca aacaccgaag ccgacacatg caagcagctg aataacaact
      781 caacccgctt cggcgggttt tttgttgcgg tacaattaag agcgttcttt cacataggag
      841 gacgcccatg aatgagagct cggttgcgcg cattcaagaa ttaaacgacg catttcgcaa
      901 gacgtttgcc ggcgggaaga tggtgatgac agcaaacgtc gccgcgttgc cggaaatggt
      961 gaaagcatct gcactggtaa aactcgcgga gtttaacgac ttcacaccgg agaatgaccc
     1021 gcacggcgag cacgactttc tcagctttga gcattgcagc cgcacattct tttggaagtg
     1081 cgactacta
//
LOCUS       DATCBB010000970         1527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_191877, whole genome shotgun sequence.
ACCESSION   DATCBB010000970 DATCBB010000000
VERSION     DATCBB010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191877"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VII37_10650"
     CDS             <1..441
                     /locus_tag="VII37_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070244.1"
                     /translation="PFNITGGTSRPGLDLIANPFAGVSHTFSAAAGGEPWVNPAAFCV
                     PGEAGCTGPTDPMGNLSRNKYYGPSFKDIDFSVFKNIPITERVRVQLRAEMYNLFNRI
                     NLASGAGSVGSNGFVSDTIGDFNGAPGLGPGEPFNMQLVAKIIF"
     gene            complement(633..971)
                     /locus_tag="VII37_10655"
     CDS             complement(633..971)
                     /locus_tag="VII37_10655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5070245.1"
                     /translation="MPVANLLVDESVKQRLLERWTNMRGHQRVTTNVKLRVEWDEGPT
                     LQNVNAVTVDVSSSGCKAVVAGDLRLHQRVKLIHAASGRRADAQVVWRGHEAWDAGFV
                     LEHPDPTFWE"
     gene            complement(1133..>1527)
                     /locus_tag="VII37_10660"
     CDS             complement(1133..>1527)
                     /locus_tag="VII37_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070246.1"
                     /translation="IEQKVSPVGVSVQQFGFIGAPRVPTVIATAITAKAQAIQQAERA
                     RNELATTQAEAAKKIAEAEGDAKSLVTRAQGEADANRIRQNSLTPQLLELRRIENNRA
                     LIDKWNGALPTVQSGQGSGMILQLPKPE"
BASE COUNT          331 a          464 c          372 g          360 t
ORIGIN      
        1 ccgttcaaca tcaccggtgg cacgtcccgg cccggcctcg acctcatcgc caatcccttc
       61 gcgggcgtgt cgcatacctt ctctgcagcg gcgggcggcg agccttgggt aaatccagcg
      121 gcgttttgcg tccccggcga ggcgggctgc accggaccca ccgatcccat gggtaacctg
      181 tcgcgcaaca aatactatgg acccagcttc aaagacatag acttttcggt gttcaagaac
      241 atccccatta ccgagcgggt cagagttcaa ttgcgcgcgg aaatgtataa cctcttcaac
      301 cgcatcaatc ttgcttccgg cgccggctcg gtcggttcca atggattcgt cagcgacact
      361 attggagact tcaacggcgc tccgggtcta ggccctggcg agcccttcaa catgcaactc
      421 gtggcgaaga ttatcttcta agccagacaa cttttactgc gtggatgcgc ggacgcattt
      481 ttcccgcatc cacgcaattt ttttgtctgc tatttgggac gattaaactc ggtaaggctg
      541 ttaatggcaa aaactacgat tgcgtgcctt gaacagagaa gtagccgaaa ggaatttgaa
      601 acaccaaaat cgggaaagag agaacaggaa aattattccc aaaacgtggg atccggatgc
      661 tcgagaacga agcccgcatc ccatgcttca tgtccgcgcc atacgacctg ggcatctgca
      721 cgacgaccgc tagcagcatg aataagcttg acccgttgat gtagccgcag atcgcccgcc
      781 accaccgctt tacaaccaga gctgcttaca tccacggtca ccgcattcac gttctgcagc
      841 gtagggccct cgtcccactc cacgcgcaat ttcacgttgg ttgtcacgcg ctggtgccca
      901 cgcatattcg tccagcgctc cagtaatctc tgcttgacgg attcatctac taacaggtta
      961 gccacaggca tgcaccacct cgacaagaac gcttcaatga ggagacttaa aagtagtccc
     1021 ggcaaaacga caggtcaata gtacttttca ttaaaatgtt aataggaaat gttaataaat
     1081 ggctttcgcc aattctttgc ctgctgctcc gcgtttgttc aaccatcagc ccttactctg
     1141 gcttcggcag ttgcagaatc atcccgcttc cttgtccaga ttgtaccgtc ggtagcgctc
     1201 cattccattt atcgatcagc gcgcgattat tttcaatgcg ccgcaattcc agaagctgcg
     1261 gagttaacga attctggcgt atccggttgg cgtccgcctc tccttgcgcc ctggtcacta
     1321 gagacttcgc gtcgccttcc gcttccgcaa ttttcttggc ggcctctgcc tgcgtggtcg
     1381 ctagctcgtt gcgcgcccgc tccgcttgct gaatcgcttg cgccttggcg gtaatcgcag
     1441 tggcaatcac cgtaggcact cgcggcgcgc cgatgaatcc gaattgctgc acgctgacgc
     1501 ccaccggcga gaccttctgt tcaatca
//
LOCUS       DATCBB010000971         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_289735, whole genome shotgun sequence.
ACCESSION   DATCBB010000971 DATCBB010000000
VERSION     DATCBB010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289735"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VII37_10665"
     CDS             complement(<1..1164)
                     /locus_tag="VII37_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY5070247.1"
                     /translation="MKEARASSEPLGSAVSLSDVVITHEDIDRAHDRERVSATRARRG
                     FRPRLRLFWLLIGPGILVILGENDGPSMLSYAATGARFGIGFFVPFVVLTFLMACVVQ
                     EMTVRLGAATHRGHAELIFDRFGPFWGWFSMVDLMVGNFLTLVTEFIAIRAGLGFFGV
                     RPAVAVIGALLVLSVALMTHRYWTWERIILGAAMFNLIFVPVALLSHPDWRTVGSALV
                     TWKPLPGGLTQETVLILLANIGATVTPWMLFFQQSAIADKGLTTRDIRMGRIDTALGA
                     ALATVAALATMLATAPLFIHHMPSANFEAAEFAQALQPFVGHWGAALFALGIFEAGMV
                     AAITISTSSAYAFGEVARRPHSLNLPFNQGKSFYMVLLLEAAAAAGLVLIPGIP"
     gene            complement(1311..1988)
                     /locus_tag="VII37_10670"
     CDS             complement(1311..1988)
                     /locus_tag="VII37_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEY5070248.1"
                     /translation="MITVSKEDYLKAILEAESEGESVISATLAHWLKVSPPAITMAVR
                     RLKKDGLVRVNTDGRVRLTAAGRKIARKLTVRHHLIERMLSEIFGMEWYKVHDEAERL
                     EHAVSPDFEARLLTKLGRGVACPHGNLTELESPASRRRRGLLLLAEAETGQNVEVSGI
                     YERDRRLLEFLEHRGIRPGARLRVAHRNYDHTLTLSTGIGEVSLGTSAAEKVWVRVSD
                     SKATSRN"
BASE COUNT          527 a          650 c          578 g          387 t
ORIGIN      
        1 cggaattccg gggatcaaaa ccaatccggc ggcggccgcg gcttcgagaa gaagaaccat
       61 gtaaaacgac ttcccctgat tgaagggcag attcaaactg tgcggacgac gcgccacttc
      121 tccaaaggcg taagcggacg aagtcgaaat cgtaatcgct gcgaccatgc cggcctcgaa
      181 aataccgagc gcgaaaagcg ccgctcccca gtggccaaca aatggctgca gcgcctgcgc
      241 gaattcagca gcctcaaagt tcgccgaagg catgtgatgg atgaagagtg gggccgtggc
      301 caacattgtt gccagcgcgg ctacagtcgc taaagccgcg cccagagccg tgtcgatgcg
      361 ccccattcga atatcgcgag tggtgagtcc cttgtcggca atcgcgcttt gctggaaaaa
      421 gagcatccac ggcgttaccg tcgctccgat gttagcgaga agaatgagca ccgtttcctg
      481 tgtaaggccg cccggcaaag gtttccaggt cacgagcgcg ctgcccacag ttctccaatc
      541 gggatgcgac agcagcgcca ccggcacaaa aatcaggttg aacatggcag cgcctaaaat
      601 aatccgttcc caggtccagt accggtgcgt catcaacgcg accgacagaa cgagcaacgc
      661 cccgatcacc gcaaccgccg gacgcacacc aaaaaatccc agtcccgctc gaatcgcgat
      721 gaattcggta accagagtta aaaaattgcc caccatcagg tcgaccatcg agaaccagcc
      781 ccagaaaggc ccgaagcggt caaagatcaa ctccgcatgt ccgcgatgag tggccgcgcc
      841 caggcgcacg gtcatctcct gcactacgca agccatcagg aaggtaagca ccacgaacgg
      901 cacgaagaag ccgatgccaa accgggcccc ggttgccgca taggagagca tgctcggacc
      961 gtcgttctct ccaagaatca ccaggatgcc ggggccgatc agcaaccaga acaaacgcag
     1021 ccgcggccgg aatccgcggc gcgcgcgggt cgccgaaaca cgttcgcgat catgcgcccg
     1081 gtcaatatcc tcatgcgtaa tgacgacatc ggatagagat acggcactgc ccagaggctc
     1141 cgatgatgcc cttgcttctt tcattcagtt taaagggccg gaagggaaga acgtcgccat
     1201 gtcgccgcag caggccatta gcgcaagtat accgcagcaa ataaactatg gttaattact
     1261 tttgggatga aaagagcctg attagattga aggactgcgg taaatcacgc tcaatttcgg
     1321 gatgtcgcct tggaatcgct cactcgaacc caaacctttt cagccgccga cgtacccagc
     1381 gaaacctcgc caatgccagt ggagagtgtc agggtgtgat cgtaattgcg atgcgcaacc
     1441 cgcaaccttg cccccggacg aatcccgcga tgctccagaa attccagcaa cctgcggtcg
     1501 cgctcataaa tgccacttac ctcaacattc tggccggttt ccgcctccgc cagtagcaaa
     1561 agtccgcgcc tccggcgact ggcagggctt tccagttcgg tcaaattgcc atgcggacac
     1621 gcgactccgc gccctaattt cgttaagagc ctggcttcga aatcaggcga aacagcatgc
     1681 tccagacgtt ccgcctcatc atgcaccttg taccattcca tcccaaaaat ttcagagagc
     1741 atgcgctcga tcagatggtg gcggaccgtt aacttccgcg cgattttccg cccagcagcg
     1801 gtcaaacgaa cgcgcccgtc ggtgttcacc ctgaccaacc cgtctttctt taagcggcgc
     1861 accgccatgg tgatagccgg aggcgaaact ttgagccagt gcgccagagt agcggaaatg
     1921 acggactcgc cctcactctc cgcctcgaga atggccttga ggtagtcttc tttggaaact
     1981 gtgatcacga gaacgctatt atacgtggaa cgattaagcg tagttaatga ggtttcaggc
     2041 tagtcaatga tcggcaggcg ggagccacgg cctgcgagct acgactaaat ggagcccggg
     2101 atggcttgct gcttcaagcg cgaggctcgc agctcgtagc tc
//
LOCUS       DATCBB010000972         1499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_386737, whole genome shotgun sequence.
ACCESSION   DATCBB010000972 DATCBB010000000
VERSION     DATCBB010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386737"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VII37_10675"
     CDS             complement(<1..309)
                     /locus_tag="VII37_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5070249.1"
                     /translation="MRLENKVALITGGTSGIGEAVAELFAREGAKVAITGRNESRGHA
                     VTTRILEAGGQAVFLRTDVRKAAECQRAVDETLRSFTRLDILFNNAGVFFPQTALECS
                     E"
     gene            complement(313..1104)
                     /locus_tag="VII37_10680"
     CDS             complement(313..1104)
                     /locus_tag="VII37_10680"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5070250.1"
                     /translation="MPLAEKTKGQLSGKVAVITGAASGIGRATAMLFACEGASIVLAD
                     MKAEAGKHVADEITQAGGRAFFETMDVTIAGDCRHLVEHAIREFGRIDILFNNAGIIR
                     RATVLDLSEEDWDRVMEVNVKSIYLLSREVIPHMQKAGGGAIINTASGWGLAGGAKAA
                     VYCASKGAVVLLTKAMAIDHGPQNIRVNCICPGDTNTGMLGEEARQLGEDSNRFLAES
                     AKRPLGRVGTPEEIAQAALYLASDASSFVTGTALVVDGGGLAGSA"
     gene            complement(1107..>1499)
                     /locus_tag="VII37_10685"
     CDS             complement(1107..>1499)
                     /locus_tag="VII37_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5070251.1"
                     /translation="TAARHMMERGSGVILQTASTNGVMGYPFYADYNATKAGVIELTK
                     SMALELAPKIRVCAVAPGYVLTPMQRAEYTDEMLAEVNRKIPLRRHAQPEEIAALFAF
                     LASDDAQNITGHVYTCDGGETAGGLASR"
BASE COUNT          290 a          453 c          414 g          342 t
ORIGIN      
        1 ttcggagcat tcgagcgcgg tctgcggaaa aaagactccg gcattattga aaagaatatc
       61 gaggcgcgtg aaggaacgca gcgtctcatc cacggcgcgc tggcattcag cggccttgcg
      121 aacgtcagtg cgcagaaaaa ctgcctgccc gccagcctca agaatgcgcg tcgtgaccgc
      181 gtgtccgcga gattcgttgc ggccggttat ggcgaccttc gcaccctcgc gggcaaacag
      241 ttcggcgacg gcttcgccga tgcctgacgt gccgccggtt atcaaagcta ctttattttc
      301 aagacgcata aattaggccg atcccgccaa acctccgcca tcgactacca gtgctgttcc
      361 cgtcacaaat gaagatgcgt cgctcgccag atacaacgcg gcctgcgcga tttcttctgg
      421 agtgcccacg cggcccagcg gacgctttgc cgactcggca agaaaacggt tgctgtcttc
      481 tccgagttgc cgtgcttctt cgcccaacat gccggtatta gtatcgccgg ggcaaataca
      541 atttacgcga atattctgcg ggccgtggtc gattgccatg gctttcgtta gtagaacaac
      601 cgcgcctttc gatgcgcagt aaactgcagc ctttgcgccg ccagccagtc cccagcctga
      661 ggcagtgttg atgatggctc cgccgcctgc tttctgcata tggggaatca cttcacgcga
      721 taggagataa attgacttta cgttgacctc catcacccgg tcccaatcct cttcgctcag
      781 gtcaagcacg gttgcgcgcc ggatgatgcc ggcgttgttg aatagaatgt cgatgcgacc
      841 aaactcgcgg atcgcgtgct ccaccaggtg gcggcaatct cctgcgatgg tcacgtccat
      901 cgtttcgaaa aatgctcgcc cgccagcttg agtaatctcg tcggctacgt gtttgcccgc
      961 ctcggccttc atgtccgcga gaacaattga agcaccttcg caggcaaaga gcatcgctgt
     1021 ggctcgacca atgccggatg ccgcgcccgt gatcacagca acttttcctg aaagttggcc
     1081 ctttgttttt tctgcgagtg gcatgcttac cggctagcca gccctccggc ggtttctcca
     1141 ccatcgcagg tgtatacgtg gccggtgata ttttgcgcgt cgtcggaggc caggaaagcg
     1201 aagagggccg caatctcttc cggctgggcg tggcggcgca gaggaatttt cctgttcacc
     1261 tcggcgagca tttcatcggt gtactcggcg cgctgcatcg gcgtcaacac gtatcctgga
     1321 gccacggcac aaactcttat tttcggcgcg agttccagcg ccatcgactt tgtcagttcg
     1381 atcacacctg ctttggtggc gttgtagtcc gcataaaacg ggtatcccat gacgccgttg
     1441 gtcgaagcgg tttgcagaat tacaccgctg ccgcgctcca tcatgtgccg cgctgccgt
//
LOCUS       DATCBB010000973         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_225955, whole genome shotgun sequence.
ACCESSION   DATCBB010000973 DATCBB010000000
VERSION     DATCBB010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225955"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VII37_10690"
     CDS             complement(<1..675)
                     /locus_tag="VII37_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5070252.1"
                     /translation="MDPKFIRNFCIIAHIDHGKSTLADRLLEMTGALSSREMHEQVLD
                     SMDLERERGITIKAKSIRLHYKAKDGNTYRLNLIDTPGHVDFSYEVSRALSACEGALL
                     VVDASQGVEAQTVANTFLAAGNNLTIVPVINKIDLPAAQTDFVKEQIETVLAIPAADA
                     LLISAKSGLGVEDVLEAIVARIPPPKGSAENPLQALIFDSWFDIYRGAVVLVRVMEGR
                     ITPHMKI"
     gene            922..1077
                     /locus_tag="VII37_10695"
     CDS             922..1077
                     /locus_tag="VII37_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070253.1"
                     /translation="MSCNPEGDCWCAELPHGPVPVVKAGETTGCLCRNCLEEQLREHE
                     REGKGVE"
     gene            1283..>2199
                     /locus_tag="VII37_10700"
     CDS             1283..>2199
                     /locus_tag="VII37_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5070254.1"
                     /translation="MAADVNKHLDRAKRFLEKNRVEDAIAAYLAVLEGAPQHQEATQA
                     LGDLYSRTDQPDLAAVYYGMLFDVLVDPRDENKAVAIYNRFLRSGRVEQPPERIARYA
                     FLQQKLNHAEEAIEQYTKAAEMFTAVSRDEDALFCLERIAQLDPENPARQLRLAEAAE
                     RISRRALAARAYLRAGQLATAHGEHEDALKLLYRAYVLAPQERSVALLYAEAKLRAGM
                     ATEAVVILDPFAGKESDATFVDTFGKALVRAGQLDRARELLERLLREKNVGTMPLFEL
                     ADAYAAAGQERNAVDIFLNLKRRMFADKK"
BASE COUNT          496 a          509 c          740 g          454 t
ORIGIN      
        1 gattttcatg tgcggcgtga tgcggccttc catcacgcgg acgagcacga ccgcgccacg
       61 gtaaatatcg aaccaggaat cgaaaatcag ggcctgaagg ggattttctg cgctgccttt
      121 gggcggcggg atgcgcgcga cgatggcttc gagaacgtct tcgacgccga gaccgctttt
      181 cgcgctgatg agcagggcgt cggcagcggg aatggcgagc acggtttcga tttgttcctt
      241 gacgaaatcg gtttgggcgg cggggagatc gattttgttg atgaccggaa cgatggtgag
      301 attgttgccg gcggcgagga aagtgttggc gacagtttgg gcttcgacgc cctggctggc
      361 gtcgacgacg aggagggcgc cttcgcaggc ggagagcgcg cgggaaactt cgtaggaaaa
      421 atcgacgtga ccgggcgtgt cgattagatt caggcggtag gtgtttccgt ctttggcttt
      481 atagtgcagg cgaatgctct tggccttgat ggtgattccg cgctcgcgct cgaggtccat
      541 ggaatcgagg acctgctcgt gcatttcgcg ggagctgagt gcgccagtca tttccagcaa
      601 gcggtcggcg agcgtggatt tgccgtggtc gatgtgcgca ataatgcaaa aattgcgtat
      661 gaactttgga tccatttagg cttataacgg caggagagtc cggtcgcttc gcgttcggtg
      721 cgtgccggga aaaatgatcg ccggtcacgc tacagcgcgc aaaaaccatg ccgcgtaaac
      781 aatgagtttg ccacaaatcc gcagttgcgt cagcccgggg acaaaaatga gcagaaattt
      841 aatgccggaa gatgacttaa gaggcgggcg ataagagaga atggtgctat ggaaacgcga
      901 tgcgctcgtt gcaaggcgct catgagttgc aatcctgaag gggattgctg gtgcgcggaa
      961 ttgccgcacg ggccggtgcc ggttgtaaaa gctggggaaa cgacgggatg cttgtgccgg
     1021 aattgtcttg aagaacaact gcgagagcat gaacgcgaag gaaaaggcgt tgagtagcct
     1081 catcagttga gacttggcag aatttgcgag ttgattacta caactgaggg aagaacgaga
     1141 tggctgagca gtgactcttt gaggcaggca tttggccgca ttctgccagc ggtatatagt
     1201 aaaagaggct gaaacactgc atgccggccg aatctgtcgc catactccat aactttttat
     1261 accgcaaagg caactctctc tgatggccgc agacgtaaac aagcatcttg accgggcaaa
     1321 acgcttcctc gaaaaaaatc gagttgagga tgctatcgcc gcttatttgg cggtgctgga
     1381 gggagcaccc cagcaccagg aggccacgca agcgttgggc gatctgtatt cgcgcacgga
     1441 ccaaccggat ctagcggcgg tttattacgg aatgctgttc gatgtgctgg tggatccgcg
     1501 agacgagaat aaggcggtgg cgatttataa ccgcttcctg cggagtggaa gggtggagca
     1561 gccgccggaa cgcatcgcgc ggtacgcgtt tctgcagcag aaattaaacc atgccgagga
     1621 agctatcgag cagtacacca aggcggcgga aatgtttacc gcggtgtcgc gggatgaaga
     1681 cgcactgttt tgcctggaac gcatcgcgca gcttgacccg gaaaatccgg cgcggcagtt
     1741 gcggctggcg gaagcggcgg aacgcatatc gcggcgggcg ctggcggctc gcgcttattt
     1801 gcgcgccggg caattggcca cggcgcatgg agaacatgag gatgcgctga aactgctgta
     1861 ccgcgcttac gtgctggcgc cgcaagaacg aagcgtggcg ctgttgtatg cggaagcgaa
     1921 gttgcgggcg ggaatggcca cggaagcggt ggtgatactg gatcccttcg caggaaaaga
     1981 gagtgacgcg acgtttgtag atacgtttgg gaaagcgctg gtacgcgccg ggcaattgga
     2041 tcgtgcgcga gaactactgg agcgcttgtt gcgcgagaaa aatgtaggga cgatgccgct
     2101 gtttgaactg gctgatgcgt atgcggctgc tgggcaagaa cgcaatgctg tggacatttt
     2161 cttaaacctg aagcggcgga tgtttgcgga caagaagca
//
LOCUS       DATCBB010000974         1864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_289809, whole genome shotgun sequence.
ACCESSION   DATCBB010000974 DATCBB010000000
VERSION     DATCBB010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289809"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VII37_10705"
     CDS             complement(<1..742)
                     /locus_tag="VII37_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5070255.1"
                     /translation="MNETYDIAVVGSGFGGSLLAMIAHRLGRSVILLEKNRHPRFAIG
                     ESSTPLSNILLESLANHYDLPGIAPLSKWGSWQQTYPQVACGLERGFTFYHHVLGTPG
                     GSDPEHQRQLLVGASPHDRIADTHWYRAEVDHLFVKEAQNIGVQYLDEVALQSASESE
                     KEIHLQGHRNNKDFSVRAKFVVDAPGPRGFLHHALALQEVALPNFPATQALFSHFRGV
                     GRFADQISDHDAELPPYPVDDAAVHHIFD"
     gene            complement(739..1716)
                     /locus_tag="VII37_10710"
     CDS             complement(739..1716)
                     /locus_tag="VII37_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070256.1"
                     /translation="MSISQLTSFYPATNLERDKWVLERRAGRETLDPQKPYLFFVEQE
                     LSAAGEILPVATIFLTNRECPWRCVMCDLWRNTLTTSVLPGAIPAQIDSALSQLPAAH
                     VLKLYNSGSFFDSRAIPVEDRPAIAERANRFDRLVVENHPALIDDHCLRFRDRLKCRF
                     EIAMGLETVHPDILPKLNKRMTLEQFSNAATFLRLNDIDLRVFVLVQPPFMQPHEAVF
                     WALRSVDFAFDCGASAVTLIPTRGGNGAMEALAGKQQFSSPRLETLETSFSYGLGLKR
                     GRVFADLWNAETIASCPVCRTQRISRLRDMNLSQVNLAPARCDSCTPAN"
BASE COUNT          452 a          488 c          539 g          385 t
ORIGIN      
        1 cgtcaaaaat gtggtgaaca gcggcgtcgt ccactggata aggtggaagc tccgcatcgt
       61 ggtctgatat ctggtccgca aaacgcccga cgccgcggaa atggctgaag agcgcctgag
      121 tagctgggaa gttaggaagc gccacctcct ggagtgcgag agcgtggtgg agaaaaccgc
      181 gaggcccagg ggcgtcgacc acaaatttgg cacgaacaga gaagtcttta ttgttgcgat
      241 ggccttgcag atgaatttct ttttccgatt ccgaagcgct ttgcaatgcg acttcgtcca
      301 gatattggac tccaatgttt tgagcttcct tgacgaacag gtgatccact tccgcgcgat
      361 accagtgcgt gtcggcaatt cgatcgtgtg ggctggcgcc gactagtagt tgacgttgat
      421 gctccggatc gcttccaccc ggggtgccga gcacgtggtg atagaaggtg aagccgcgtt
      481 cgaggccgca ggccacctgc ggataagtct gttgccaact gccccacttc gatagcggcg
      541 caattcctgg caggtcataa tgattcgcca agctctccag caggatgttc gacaaaggtg
      601 tggaagattc gccgatggca aaccgcgggt gcctgttttt ctcgagcaga attaccgagc
      661 gccccaaccg gtgcgcaatc atagccagca ggctgccgcc aaagcctgag cccactacgg
      721 caatgtcgta agtttcgttc aatttgcggg tgtgcaactg tcgcaacgag ccggcgcgag
      781 attaacttga gagagattca tgtcacgcaa gcgcgagatg cgttgtgttc gacacacggg
      841 acaagaggca atcgtttccg cattccacaa atccgcaaag acacgtccgc gtttcaagcc
      901 caagccataa ctgaaggacg tctccagggt ttccaatcgg ggcgatgaaa attgttgctt
      961 accagccaga gcttccatcg cgccattgcc gccgcgcgtt ggaatgagcg tgactgcgct
     1021 cgcgccgcaa tcaaaagcga aatcgacaga gcgcagggcc caaaaaacag cctcgtgagg
     1081 ctgcatgaat ggtggctgta caagaacgaa tacgcgcagg tcgatatcgt tcaagcgcaa
     1141 gaaagtcgcg gcgttcgaaa attgttccag cgtcatgcgc ttattgagtt tcggcaaaat
     1201 gtccgggtga accgtttcca gccccatagc tatctcgaaa cggcatttca gacgatcccg
     1261 aaagcgcaag cagtgatcat cgatgagagc aggatggttt tcgacgacca ggcggtcgaa
     1321 gcgattagcg cgctcagcga ttgcggggcg gtcttcgacg ggtatcgcgc gagaatcaaa
     1381 aaagctaccg ctgttgtaga gcttcagtac gtgggctgca ggcagttgcg acaaggcgga
     1441 atcgatctgt gcaggaatcg cgcctggcag aaccgaggtg gtcaaagtat tgcgccagag
     1501 atcgcacatc acgcaacgcc atggacactc gcgattggtc agaaaaattg tggctacggg
     1561 aagaatttcc cccgcagccg acaactcctg ttcgacaaaa aacaagtagg gtttctgcgg
     1621 atctagagtt tcgcgtccgg cacgtcgctc aagtacccac ttgtcgcgtt ccagattggt
     1681 tgcgggataa aacgatgtca gttggcttat agacaagatg ctcacaaata cagcgacaaa
     1741 aattgtttgc ggtaatccgg ttcccgcgaa ggcagttggc gacgcatatc gacttccggc
     1801 agcgcgccat gttgaaaccg gcgcttcgga aaaacccgca tacgcatccc ggttacagat
     1861 cgga
//
LOCUS       DATCBB010000975         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_386786, whole genome shotgun sequence.
ACCESSION   DATCBB010000975 DATCBB010000000
VERSION     DATCBB010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386786"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /gene="carB"
                     /locus_tag="VII37_10715"
     CDS             complement(<1..1434)
                     /gene="carB"
                     /locus_tag="VII37_10715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5070257.1"
                     /translation="MPKRTDIKKIMIIGSGPIVIGQACEFDYSGAQACKALRSEGYEV
                     VLVNSNPATIMTDPEIAQRTYIEPLTKEYLEEIISREKPDALLPTVGGQTALNLAVEL
                     AESGILEKHGVEMIGASLRAIKVAEDRLWFKDACRKIGLEVPASALVNNAQDALRLCD
                     QLGFPLVIRPSFTLGGTGGSIAYNREEFGEAISHALDMSPVHEALVEESVLGWKEYEL
                     EVMRDYRDNFVVICTIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAAAIIREVG
                     VETGGSNVQFAINPENGKMTVIEMNPRVSRSSALASKATGFPIAKIAARLAVGMSLDE
                     IPNDITKQTPACFEPTIDYVVVKIPKWQFEKFPGADSTLGTQMKSVGEVMAIGRTFKE
                     ALQKGIRSLEPSTPWRAPTETPDSLLREKLATPRPDRIHWLLTAVERGLSSEEICELT
                     KIDPWFIQQIEEIIAMNRRAAKVTVETA"
     gene            complement(1497..2663)
                     /gene="carA"
                     /locus_tag="VII37_10720"
     CDS             complement(1497..2663)
                     /gene="carA"
                     /locus_tag="VII37_10720"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY5070258.1"
                     /translation="MTEVQHRPATLALEDGRIFNGRAAGAITRRGGEVVFNTSLTGYQ
                     EVFTDPSYAGQIVCLTYPHIGNVGANIDDEEAPRPYIESLVVREFSQMSSNWRSSEPA
                     QLYLNRHQIPVIWDIDTRALVRHIRSVGALRGVVATDGTAAEQLIHEAKQLPKMAGQE
                     LASRVSCHKAYSWDKGSIELASAPWTEQSASSGTQHETPRYRVAAYDYGIKQNILRML
                     IDHHCEVLVVPAQTSAEEVLGLGPDGVFLSNGPGDPEPVDYAVGNIRKLIGKVPVFGI
                     CLGHQLCGLALGGKTFKLKFGHHGSNHPVHNLLTQKIEITAQNHGFCVDPESLPSSDV
                     EITHVNLNDHTNEGMRHKSLPLFSVQYHPEASPGPHDSHYLFTQFTDMMKENRR"
     gene            complement(2763..3413)
                     /gene="lepB"
                     /locus_tag="VII37_10725"
     CDS             complement(2763..3413)
                     /gene="lepB"
                     /locus_tag="VII37_10725"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY5070259.1"
                     /translation="MEHSDEKPGASSVNSNSAAGISPSTPPNTSSEQTPSLLSLRHEI
                     RVWTRDLLIAIGLALVIIVFLYQPVKVEGTSMAPLLSDQERIFINKFVYRFEAIHRGD
                     VVVFWYPLDRSKSFIKRVIALPGETVTIRQGVVTVNGTVVPEPYVPPQYEDVSDYGPT
                     RVPADSYFVMGDHRISSNDSRVFGPVPSQFIYGRAVFAYWPVDHFGSLAMTEAESK"
BASE COUNT          808 a         1079 c          892 g          830 t
ORIGIN      
        1 cgccgtttcc accgtgactt tggcggcgcg gcgattcatg gcgattattt cttcaatctg
       61 ttgaatgaac cacggatcga ttttagttag ctcgcaaatt tcttcagacg acaaaccccg
      121 ttccaccgcc gtaagcaacc aatgaatccg gtccggccgc ggtgtcgcca atttttcccg
      181 caacaaggaa tctggcgttt ccgtgggcgc gcgccatggc gtgcttggct caagtgaccg
      241 tatacccttc tgcagcgcct ctttgaaggt tcgtccgatg gccatcactt cgccgaccga
      301 tttcatctgc gttcccagcg tgctgtcagc gccagggaac ttttcgaatt gccacttggg
      361 gattttcacc acgacataat cgatagtcgg ttcgaaacac gcgggagttt gcttggtgat
      421 gtcattggga atttcatcca gcgacatccc caccgccaaa cgcgcagcaa tcttggcgat
      481 cgggaatcca gtagctttgc tggccaatgc cgagctgcgc gacacgcgcg gattcatctc
      541 aataacggtc atcttgccgt tttcaggatt gatcgcgaat tgcacgttgg agccgcccgt
      601 ctccacgccc acttcgcgta tgatcgcggc cgccgcgtcg cgcatgcgct gatattcctt
      661 atccgtcagc gtctgcgccg gcgccacggt aatggagtcg ccggtatgca ctcccatggg
      721 gtcaaaattt tcgatggtgc agatgaccac gaaattgtcg cggtaatcgc gcatcacttc
      781 tagttcgtac tctttccagc ccaaaaccga ttcttccacc agcgcttcgt gcaccggact
      841 catgtccagc gcgtgcgaaa tcgcttcgcc aaattcctcg cggttgtagg cgattgagcc
      901 tccggtgccg cccaatgtaa aagatggacg aatcaccaac ggaaatccca gttgatcgca
      961 aagccgcagt gcgtcctgcg cattattcac gagcgcggag gcgggaactt ccaagccaat
     1021 tttgcggcaa gcatccttga accacagccg gtcctcggcc acctttattg cgcgcagcga
     1081 agccccaatc atttccacgc catgcttttc gaggatgccg ctctcagcca actccaccgc
     1141 aaggtttaac gcggtctggc cgcccaccgt cggaagcaac gcatcaggct tttctcggga
     1201 gataatttct tccagatatt ccttggtgag gggctcgatg tacgtccgct gcgcaatttc
     1261 cggatccgtc atgatcgtgg ccggatttga attcaccagc accacttcat aaccttccga
     1321 acgcagcgcc ttgcacgctt gcgcgccgga ataatcaaat tcgcaggcct gcccgattac
     1381 aatcggcccg gaaccgatga tcatgatttt cttgatgtcc gttcgcttag gcatccgctt
     1441 gaacaggctt ccagcctgaa ttgttttctt tcctgctcat aaccagaact gaaatcctac
     1501 cgccggtttt ccttcatcat gtccgtaaat tgcgtgaaca agtaatggga atcgtgcgga
     1561 cccggcgaag cctccggatg atactgcacg ctgaacaatg gcaaagattt gtggcgcatc
     1621 ccttcattgg tgtgatcgtt caaattcacg tgcgtgatct ccacatcact cgaaggcagc
     1681 gactccggat ccacgcaaaa tccgtggttc tgcgcggtaa tttcgatttt ttgcgtaagc
     1741 aagttgtgta ccggatgatt cgaaccgtga tgcccaaatt tcaacttgaa agttttaccg
     1801 cccagcgcca acccgcacag ctggtgccct aaacaaattc caaacaccgg aactttgccg
     1861 atcagcttgc gaatatttcc caccgcgtaa tcgacaggct ctggatcccc cgggccattc
     1921 gacaaaaaaa cgccatccgg ccccagacca agcacctctt cagcagaagt ttgcgccgga
     1981 accaccagca cttcgcagtg atgatcgatc aacatgcgca aaatgttttg tttgattccg
     2041 taatcgtacg ccgccacgcg atatcgcggc gtctcgtgct gagttccaga ggatgccgac
     2101 tgctcggtcc acggcgcaga cgccagctcg atcgatcctt tatcccatga gtacgctttg
     2161 tggcaactga cgcggctggc taattcctgc ccggccatct tcggcaattg ttttgcttcg
     2221 tggataagtt gctcggcagc cgtgccgtcg gtcgctacca cgccgcgcaa cgcaccaaca
     2281 ctgcgaatgt gcctcaccag cgcgcgcgta tcaatgtccc aaatcacggg aatctggtga
     2341 cgattcaaat agagctgtgc aggctccgac gagcgccagt tggaagacat ctgcgaaaat
     2401 tcgcgcacca ccagcgattc gatgtacgga cgcggagctt cctcatcgtc gatattagcg
     2461 ccaacatttc caatgtgtgg ataagtgaga cagacaatct gcccggcgta actgggatcg
     2521 gtgaaaactt cctggtaacc ggtgaggctg gtattaaaaa cgacttcgcc gccccggcgc
     2581 gtgattgcgc cggccgcgcg gccgttaaat atgcgcccgt cttccagcgc aagtgtggcg
     2641 ggtcgatgct ggacttccgt cagtggagcc ccctgttttt atcatcgcag tcctaatcac
     2701 gcgaaacaag tgactactag tacgtcccag tttcgaactc gaagtatgaa actggacttc
     2761 aactatttgc tttccgcttc cgtcatcgcc agcgaaccaa aatgatccac cggccaatac
     2821 gcaaacacgg cgcgcccata aataaattga ctaggcaccg gcccaaaaac gcgtgaatca
     2881 ttcgaactga tgcggtgatc gcccatcaca aaatagctgt ccgccggcac acgcgtcggc
     2941 ccgtaatcgc tgacgtcttc atattgcggc ggaacgtaag gttcaggcac caccgtgccg
     3001 ttcaccgtca ctaccccttg ccgaatcgtc accgtctcgc ccggcagcgc aattacccgc
     3061 ttgataaacg acttcgagcg atccagcgga taccagaaca ccaccacatc cccgcgatgg
     3121 atggcctcaa aacggtatac aaacttgttg atgaaaattc gttcctgatc ggaaagcagc
     3181 ggcgccatgc tggtgccttc cactttcacc ggctggtaca agaaaacaat gatgaccagc
     3241 gccaggccaa tggcgataag caaatcgcgg gtccacacgc gaatctcgtg gcgcaaagaa
     3301 agtaacgacg gagtttgttc tgaagaagta ttcggaggag tactgggaga aataccggca
     3361 gcgctattcg aattcacgct cgatgcgcca ggtttctcgt cggagtgttc cattttgctg
     3421 cctcgtattt ataacatgag catcgcagaa cgcgccagat ttccgtttcg tctgcattag
     3481 cgcatcaacg ccccaacccg gaagggagag tacttggtac tctggcgacg ccccaacagc
     3541 gggcgcacaa ttttgattct gttccctgta ttaagtaaga ttccccccga ccgagttccc
     3601 gttgcctat
//
LOCUS       DATCBB010000976         1141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_226017, whole genome shotgun sequence.
ACCESSION   DATCBB010000976 DATCBB010000000
VERSION     DATCBB010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226017"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1065
                     /locus_tag="VII37_10730"
     CDS             286..1065
                     /locus_tag="VII37_10730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5070260.1"
                     /translation="MPPTFSSRRYNFDWRGFITPGVKLIVLICSGVFLVQTLADILLG
                     RDRAYHLIVQTFGLIPDAVIHGLRIWQPATYIFLHGGLFHLLINVLMLWMFGRELEQV
                     WGKRRFLNYFFLCGVGAGLIEVIIKTIPTLFGQPASLVPTIGASGAIFGILIANAILF
                     PDRRIWLIPLPVTIPMRPYVAVMAAIEFFGTLSSGGDNVSHLCHLGGMLVGYIYLRRG
                     SFLYNVRNSVTDWRVQRNKKKFQVYMNKQKDPPSRPDNWVN"
BASE COUNT          220 a          374 c          291 g          256 t
ORIGIN      
        1 tcgtgcctcc ccgcgaccgc gatttcccca ttggacccga agaaccaact gaacccaatg
       61 agcccggcgc aggttcacct cccgtgccaa gttcggcctt cacggtcggc ccgcctgcgg
      121 cggcagggcc gtcgtactcg ggatctccga tgcgcagcgt tcccttgtcc gccaacctgg
      181 gcaggcctgc ctaccgtggt agggtccctc gcacacattc gcgaatatca tcaacatgga
      241 aaccgcgctt tgacgccact gcgtcgcgcg ccgtacactg cgcttatgcc tccgacgttc
      301 agctcgcgcc gctacaactt cgattggcgc ggcttcatca cccccggcgt caagctgatc
      361 gtattaattt gttccggcgt ttttctggtg caaacgctgg cggacatttt gctcggacgc
      421 gaccgcgcgt accacttaat cgttcagacc tttggtctga ttcccgacgc ggtgattcac
      481 ggcctgcgca tatggcagcc cgccacctac atcttcctgc acggcggcct cttccattta
      541 ctgatcaacg tgctgatgct gtggatgttt ggtcgcgaac tggaacaggt gtggggcaag
      601 cgccgcttcc tgaattattt tttcctgtgc ggcgtcggcg cgggactgat cgaagtaatt
      661 ataaaaacga ttccaacgct tttcggccag cccgcctctc tggtgcccac catcggcgca
      721 tccggcgcga tcttcggcat actcatcgcc aacgcaattt tatttcccga ccggcgcatc
      781 tggctgattc ccctgccggt gacaattccc atgcgcccct acgtagcggt catggccgcc
      841 atcgaatttt ttggcacgct cagcagcggc ggcgacaacg tgagccatct ttgccacctg
      901 ggcggcatgc tggtcggcta catttatttg cgccgcggct cgttcctcta caacgtgcgc
      961 aacagcgtca ccgactggag agtgcagcgc aacaagaaaa aattccaagt ctacatgaac
     1021 aaacagaaag accccccgtc gcgccccgac aactgggtca attaagcgat aaaattttcc
     1081 ggagatctcg cagtaacact cgaggtgtaa ctagtttcaa accatgagct tccgccactc
     1141 g
//
LOCUS       DATCBB010000977         1702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_289866, whole genome shotgun sequence.
ACCESSION   DATCBB010000977 DATCBB010000000
VERSION     DATCBB010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289866"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..>1702
                     /locus_tag="VII37_10735"
     CDS             154..>1702
                     /locus_tag="VII37_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5070261.1"
                     /translation="MSFNLQSVWNRVRVGRIRILWLVLGAMLLVSAVPIGLYHRQVLH
                     LSQGKLTDNERVQQTDLTRSLSQEIQFFESNLTQQLMSERQILTLTGLIDNVEDPVSE
                     PKVTRLLENFVDSNRTTFVYLTAVGRSGKGTSASQGNFRAEQDPFVAKALQRAFVTCM
                     QSQQLNVVKFRSDPLALAPDNRPAFVIAVPLRDAGDQFTGMLAAVVSLDTILHRLQEA
                     SVRGRTVFVVDHYGHIVAHKDTKNFVPGADASGNSLVKQVMALPQELRNTETVRFSEK
                     EKNHTVEMIGTYSTFPEVNWAVIAERSLNEARSDAGMDELNRQALAFVSFVVLAAIGL
                     GYFFAVGISGPIRGLADSTRAISRGEFHQRSPVRGATEISELAENFNKMAGDIEEYIE
                     RLKEAAEENRELFLGSIRMLAAAIDEKDPYTRGHSGRVAKYSMLVGQELGMSVEDLDT
                     LRISALLHDVGKIGVEDRVLKKPGALTSEEFDLMKQHTIKGANIMRPVSQLKDMLPGI
                     ELHHEHMD"
BASE COUNT          373 a          477 c          504 g          348 t
ORIGIN      
        1 gattcgggga cgccatgacg caggccgagc ttaacaactg gcgcgttcta gctccgtcga
       61 agagtagtac cttcgttcca gttgcacatg cgctacgcgc tgctgttgtc agccacttcc
      121 cgtcgctggc ttcacctaaa atagagcctg gttatgagct tcaaccttca aagcgtctgg
      181 aatcgtgtgc gcgttgggcg cattcgcatc ctatggttgg tgctgggcgc gatgctgttg
      241 gtcagcgcgg tccctattgg cttatatcac cgccaggtac ttcacctcag ccaggggaaa
      301 ttaacggaca acgaaagagt gcagcagacc gatctgaccc gctcgctttc ccaggaaatt
      361 caattcttcg aatccaatct gacccagcaa ttgatgagcg agcggcaaat tcttacgctc
      421 accgggctga tcgacaatgt cgaagatccc gtctcggaac caaaagtaac gcgcttgctc
      481 gagaacttcg tcgacagcaa ccgcactact tttgtttacc tcactgccgt gggccgctcc
      541 ggcaaaggca ccagcgcatc acaagggaat tttcgagcgg agcaggatcc ctttgtagcc
      601 aaagcgctgc aacgcgcttt cgtaacttgc atgcagtcgc agcaactgaa cgtcgtgaaa
      661 tttcgcagcg atccgttggc gctggccccg gataaccggc cggcgttcgt gattgcggtg
      721 ccactgcgag atgccggcga tcagtttacc gggatgcttg ccgccgtcgt ttcgctggac
      781 accattctgc atcgcttgca ggaggccagc gttcgcggcc gcaccgtgtt cgtcgtcgat
      841 cactatgggc atatcgtggc gcataaagac acgaagaact tcgtgcccgg cgcggatgcc
      901 agcggcaatt cgctggttaa gcaggtgatg gcgctgccgc aggagttgcg caacacggaa
      961 acggtgcgct tctccgagaa agaaaaaaat cacaccgtgg aaatgattgg aacctacagc
     1021 acatttccag aggtcaactg ggcggtcatc gctgagcgca gcttgaacga ggcgcgttcc
     1081 gacgccggta tggacgaatt gaatcgccag gctctggcat tcgtaagttt tgtagtgctg
     1141 gcggccatcg gcctgggtta tttcttcgct gtaggaatca gcgggccaat ccgcggcctc
     1201 gcggattcca ctcgcgctat cagccgcggt gaatttcacc agcgctcgcc ggtgcgcggc
     1261 gcaacggaaa tcagcgagct ggctgaaaac ttcaacaaga tggcaggcga catcgaggag
     1321 tacatcgagc gcctgaagga agccgcggaa gaaaatcgcg agctgttcct tggctcgatt
     1381 cgaatgctcg ccgcggccat cgacgaaaag gatccgtaca cgcgcggtca ttccggccgc
     1441 gtggcgaaat attccatgct ggtggggcag gagctgggca tgtcggtcga ggatctggac
     1501 acgctgcgta tttccgcgct gctgcacgac gtgggaaaaa tcggcgtgga ggaccgggtg
     1561 ctcaagaagc ccggcgcgct gaccagcgaa gaattcgacc tgatgaagca gcacaccatc
     1621 aagggcgcga acatcatgcg gccggtttcg caattgaaag atatgctgcc gggaatcgag
     1681 ttgcaccacg agcacatgga tg
//
LOCUS       DATCBB010000978         1084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_226087, whole genome shotgun sequence.
ACCESSION   DATCBB010000978 DATCBB010000000
VERSION     DATCBB010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226087"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VII37_10740"
     CDS             <1..430
                     /locus_tag="VII37_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEY5070262.1"
                     /translation="RGLATGDLDNDGRIDAVVTTNDGHVAILHNETQTENHWITLQLR
                     GHKSNRDGIGAEVKITTPDGSQWATVSTAGSYLSSNDKRVHFGLAGYAKANTIQIHWP
                     SGIVQTLLDVQGDRIVQVDEPSTSSSADDSAIPPGNSNPK"
     gene            854..>1084
                     /locus_tag="VII37_10745"
     CDS             854..>1084
                     /locus_tag="VII37_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070263.1"
                     /translation="MAADAEPLARQYLDKHPDSATGHFLLGYILFAEIREHPGTNSQA
                     ARLAYTEKIKGAPGDKFTEEKARASLAEYTEGA"
BASE COUNT          241 a          303 c          297 g          243 t
ORIGIN      
        1 gcgcggattg gccaccggag atctcgacaa tgatggtcgg atagacgcag tggtgaccac
       61 caacgacggc catgtagcga tcctgcacaa tgaaacgcag accgaaaatc attggatcac
      121 tttgcaactc cgaggtcaca aaagtaatcg cgacggaatt ggcgctgaag tcaaaattac
      181 tacgccggat ggctcgcaat gggccactgt cagtacggcg ggcagctacc tgtcgtccaa
      241 cgacaaacgc gtacacttcg ggttggccgg ttacgcgaag gccaacacaa tccaaattca
      301 ctggcctagc ggcatcgtgc agactttgct tgatgttcaa ggcgatcgca tcgtccaggt
      361 ggacgagcct tcgacgagct cttctgccga cgattcggcc atcccgccgg gtaattcgaa
      421 tccaaagtga tgcgctgtat tcaaaacttt tcgtactatt tccgccggca ttgtatcttc
      481 gcgatgactc tggccttgct gatgactgca tccccatcgc gtggacaagt tgagttttaa
      541 caccagtcga atgtgcaaaa tttgcgcatg tggccaccgc tgcatgcgca aattgcattt
      601 cagttgctag caattcggac ggaagctgcc gaaaaactgg cctgcgtgtt ctgctagact
      661 ttgcggcggt atgttgaact tagttgcgag ccttctcttg ggcggttggt tggtctgctt
      721 cacggccgcg cctgtcgttc agatgcagcc gcgatctgct tcgccgctgg catggctttc
      781 ctcttctgaa gcgggccgct cacaggagcc ccgcgacttg ctgtccgagg cgaagtcttt
      841 ggcggagaaa aacatggcgg ctgacgcaga accgctggcg cggcaatatc tcgacaagca
      901 tccggattcg gcgacaggcc actttctcct tgggtatatc ctcttcgccg aaattcgcga
      961 acatcctggt actaattcgc aggcggcacg cctggcttac accgagaaaa tcaagggcgc
     1021 gcctggcgac aaatttaccg aagaaaaggc ccgtgcttct ctggccgaat ataccgaagg
     1081 cgca
//
LOCUS       DATCBB010000979         1161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_128071, whole genome shotgun sequence.
ACCESSION   DATCBB010000979 DATCBB010000000
VERSION     DATCBB010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128071"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VII37_10750"
     CDS             complement(<1..598)
                     /locus_tag="VII37_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5070264.1"
                     /translation="MHNVVIIGSGCAGLTAAIYAARANLKPLVIDGHEPGGQLTLTTM
                     VENFPGFPDGIMGHELIENMRKQAQRFGAEFNAGIVTDVDLSKRPFVVTAGAERYETK
                     TLIIAAGASARLLGLPEERSLLGSGVSTCATCDGFFFRGKAIAVVGGGDTAMEEANFL
                     TRYASHLYLIHRRNEFRATKIMIDRVRANEKVEIVTPAV"
     gene            811..>1161
                     /locus_tag="VII37_10755"
     CDS             811..>1161
                     /locus_tag="VII37_10755"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HEY5070265.1"
                     /translation="MDAQYPIGKMEMPKEITPARRQEAIDSIARTPKNLREAVRDLSE
                     AQLDTPYREGGWTVRQVVHHVPDSHLNAYIRLRLALTEDKPTIKPYAEADWAELLDAK
                     SGPIEVSQRLLDALH"
BASE COUNT          258 a          301 c          376 g          226 t
ORIGIN      
        1 ccaccgcggg ggtgacgatc tcaacttttt cgttggcgcg aacgcgatcg atcatgatct
       61 ttgtggcacg aaattcgttg cggcgatgga tcaggtacaa gtgcgaagcg tagcgggtga
      121 gaaaattcgc ttcttccatg gcggtatcgc cgccgccaac cacggcgatg gccttgccgc
      181 gaaaaaagaa accatcgcag gtcgcgcagg tggaaacgcc gctgcccagc agggagcgct
      241 cttcaggaag gccgaggaga cgcgcggagg ctcccgcggc aatgatgagg gtcttggttt
      301 cataacgctc agcgccggcg gtgactacga acgggcgctt ggaaagatcc acgtcggtga
      361 caatgcctgc attgaattcc gcgccgaatc tctgcgcctg tttgcgcata ttctcgataa
      421 gctcatgacc catgatgccg tcgggaaaac cggggaaatt ctcgaccatg gtggtaagcg
      481 ttagctgccc gccgggctcg tggccgtcga tgaccagggg tttcagattg gcgcgcgcgg
      541 cgtaaatcgc ggcggttaag ccggcgcagc cgctgccgat gatgactacg ttgtgcatgc
      601 tagttcgctc ccgtatttga ttctcgcacg tgcccggggg atacaatgac gcaaaacggg
      661 tcattctacc tgaatagggt gcctccggtg tgcgatttgg ggaggtagcg gccaaattgg
      721 acagcaaatt gtagagatgc cgcgttgatc aggccaaagg gttaacttta tagttcaaca
      781 cttgtgcagg attgcaaaag gagcaagacg atggatgcgc aatatccgat tggaaaaatg
      841 gaaatgccga aggagattac cccggcaagg aggcaggagg ccatcgactc gatcgcgcgc
      901 acgccgaaaa atttgcgcga agcggtgcgc gacttgagcg aggcgcaact ggatactcct
      961 tatcgtgagg gcggctggac cgtgcggcag gtggtgcacc acgtgccgga cagtcacctg
     1021 aacgcgtata tccggttgcg cctggcgttg acagaggaca agccgaccat caagccatat
     1081 gcggaagccg actgggcgga gttgctggat gcgaagtccg ggcccatcga agtttcgcaa
     1141 aggctgctgg atgcgctgca c
//
LOCUS       DATCBB010000980         1895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_161208, whole genome shotgun sequence.
ACCESSION   DATCBB010000980 DATCBB010000000
VERSION     DATCBB010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161208"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..563)
                     /locus_tag="VII37_10760"
     CDS             complement(30..563)
                     /locus_tag="VII37_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070266.1"
                     /translation="MSIDWPHAPVHRLDSDGIFMVTAATLHKNHLFADAGKLTLLEHG
                     LLSLAKKYQWQLEAWAVFTNHYHFVARRKRESASLKKLLTHLHADSARDLNRIDEEIG
                     REVWFNFWDTKLTYERSYLARLNYVHQNPVKHGLVTIANQYRWCSAAWFERTVSPATA
                     KTIYGFKTDKLNIHDDF"
     gene            complement(675..1526)
                     /locus_tag="VII37_10765"
     CDS             complement(675..1526)
                     /locus_tag="VII37_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070267.1"
                     /translation="MPVSATPQTPQSPSPMIDHTRPHPRITQTEGAGRRVDLKSLKGA
                     TLFIGPRVNPNNPVPLLIHFHGAPWLIEQHVARYLPNAALITVQLGSGSSVYRKPFEQ
                     TNLFHDLLAEAGQALSIKRGWASITLSGFSAGYGAVREILRRPEYFALVNNVLLLDGL
                     HASYSPEGKLIADGGTIQASDIDAFVNFAREAVAGRKTFVVTHSEIFPGAYASTTECA
                     DYLLAQLTLKRLPKLANAPMGMQQLSAVDAGGFHLRGYAGNTAQDHVDFLHAMPAWFG
                     LALGTRK"
     gene            1543..>1895
                     /locus_tag="VII37_10770"
     CDS             1543..>1895
                     /locus_tag="VII37_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070268.1"
                     /translation="MSKAVRSMGGSIAEEAKFADRIYKMNRITKAGMARLCRSHLVNP
                     VQLHENPPVAQKLLTPISARSLAQLACSIGPGWKLKNESGLPEPGRDHAAALEDQFGF
                     TLQKKRAEFQHPLSCR"
BASE COUNT          425 a          495 c          557 g          418 t
ORIGIN      
        1 gctaacaagt tagcgcactc caaataagat cagaagtcgt cgtggatgtt tagcttgtcc
       61 gtcttaaaac cataaatcgt ttttgctgta gctggcgaaa cggttcgttc gaaccatgcc
      121 gcggaacacc agcgatactg attagctatc gtcaccaacc catgctttac aggattctgg
      181 tgaacatagt tgagtcgggc caggtaggag cgttcgtaag taagtttggt gtcccagaag
      241 ttgaaccaca cctctctacc gatctcctcg tctatgcgat taaggtccct cgccgaatcg
      301 gcgtgaagat gcgttagcaa ttttttcaaa ctcgctgatt cacgtttgcg tcgcgcgaca
      361 aagtgatagt gattcgtaaa gaccgcccag gcctcgagct gccattgata ctttttcgcc
      421 agcgagagta agccgtgttc gagcaaggtt aacttgcccg catctgcgaa cagatggttc
      481 ttatggagcg tcgcggccgt gaccatgaat atgccgtccg aatccagccg gtggacaggc
      541 gcgtggggcc aatcgatgga cattattgcc ggcgagtata ctcgggaggg caagtaagaa
      601 taattgctac ctggttagag agcggcgaca agtcgccgct taacaaagcg ctgacaagtc
      661 agcgcactcc aaatttattt gcgtgttccc agcgccaacc caaaccatgc gggcattgcg
      721 tgcaggaagt cgacgtgatc ctgggcggtg tttcccgcat agccgcgcag gtgaaagccg
      781 ccggcatcca ccgcgctaag ttgttgcata cccatcgggg cgttagctag ttttggaaga
      841 cgtttgagag ttagttgagc cagcaggtaa tccgcgcact cggtggtgct ggcgtacgcg
      901 ccgggaaaga tttcggaatg cgtcaccaca aaagtttttc tgccggcaac tgcttcgcgg
      961 gcgaagttaa cgaacgcatc gatgtctgac gcctggattg tgccgccgtc ggcgatcaac
     1021 ttgccctcgg gcgaatagct cgcgtgcagg ccatcaagca gcagcacgtt gtttacgagc
     1081 gcgaagtatt ccggccggcg caggatctcg cgcacagcgc cgtaacctgc cgagaatccg
     1141 gagagtgtga tcgacgccca gccgcgtttg atcgacaacg cctgccctgc ttctgcgagt
     1201 aagtcatgga agagatttgt ttgttcaaaa ggcttgcggt aaacgctcga gcccgagccg
     1261 agctgcacgg tgatcaaagc cgcgttgggc aggtaacgag ctacgtgttg ttcgatcaac
     1321 cacggcgcgc catggaaatg aatcagcaaa ggcactgggt tattcgggtt gacgcgtggg
     1381 ccaatgaaga gcgtcgcgcc ctttaacgat ttcaaatcca cgcggcggcc tgcaccttcg
     1441 gtttgcgtga tgcgtggatg cggccgcgtg tgatcgatca tcggcgaagg gctctgcggg
     1501 gtttgcggtg tggccgaaac aggcaccaac aggatcaatg ttatgagcaa ggcggtgcgg
     1561 agcatgggcg ggagtatagc ggaggaggcg aagtttgcgg acaggattta caagatgaac
     1621 aggattacca aagctggtat ggcccggctt tgtcggtctc atcttgttaa tcctgtccaa
     1681 ctgcatgaga acccgcccgt cgcccaaaag ctactgaccc ccatttcggc gcgttctctg
     1741 gcacagctcg cgtgcagcat cgggccaggc tggaaactta aaaacgaatc cggattgccg
     1801 gagcctggtc gggatcacgc ggcggctctt gaggatcagt tcggtttcac tctgcaaaag
     1861 aaacgcgccg agttccagca tccacttagt tgccg
//
LOCUS       DATCBB010000981         1106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_451220, whole genome shotgun sequence.
ACCESSION   DATCBB010000981 DATCBB010000000
VERSION     DATCBB010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451220"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VII37_10775"
     CDS             complement(<1..195)
                     /locus_tag="VII37_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070269.1"
                     /translation="MPRIDRLASVYLSHPLARLMRAVSATRVPILMYHSVSDSLFGKS
                     HPYYQINCSPPVFARQMRWLR"
     gene            complement(294..>1106)
                     /locus_tag="VII37_10780"
     CDS             complement(294..>1106)
                     /locus_tag="VII37_10780"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5070270.1"
                     /translation="KTKKLTERKRGLRVAAIVPVHLYGQTADMDPIMELAAKYNLVVI
                     EDACQAHGAEYFSKKENRWQKAGSIGLAAAFSFYPGKNLGACGEAGATTTNDEALAKR
                     MKMIRDHGQAKKYYHDIEGYNGRLDSIQAGWLSVKLRHLAKWNQSRREHAHRYHELFA
                     DIKGAVTIPVEAAWTKGVYHLYVVRVADREALQAHLAEAGIGTGIHYPIPLHLQKAYQ
                     HLNYEKGDFPVTERVAMEIVSLPMYPQMKQEQLALVTTSVKEFATSKIGAAR"
BASE COUNT          229 a          309 c          299 g          269 t
ORIGIN      
        1 ccgcagccag cgcatctggc gcgcaaacac cggaggcgag cagttgatct ggtaataagg
       61 atgagatttc ccgaacaaac tgtcggaaac actgtggtac atgagtatcg gcacacgcgt
      121 tgccgacacg gcgcgcatta accgcgcaag cgggtgggac agatacacgg aggccaagcg
      181 gtctattcgc ggcataaaaa actcagtgga atacattttg tacgacgcgc cctcgacgcg
      241 cagttaatga tggacaaagg tctaacgacg ctggtttgct gcgcggaccc tgcttagcgc
      301 gcggcgccga ttttggaagt ggcaaattcc tttacgctgg tggtcaccag ggccagttgc
      361 tcttgcttca tctgcgggta catcggcagt gagacgattt ccatagccac tcgctcggta
      421 accgggaaat cgcccttttc atagttcaag tgctgataag ccttttgcag atgcagcggt
      481 atgggatagt gaatcccggt tccgatgcct gcttccgcca gatgcgcttg caaagcttcg
      541 cgatccgcta ctcgcacgac atataagtgg tagacgcctt tcgtccacgc tgcttcaacc
      601 ggaatcgtca ccgcgccttt gatatcggca aataattcgt gatagcggtg cgcatgttca
      661 cggcgcgatt ggttccattt cgccagatgg cgcaatttaa cggaaagcca tcccgcttgg
      721 atcgagtcca aacggccatt gtagccttcg atatcgtgat aatacttttt tgcctggccg
      781 tggtcgcgga tcatcttcat gcgcttggcc agggcctcat cgtttgtggt ggtggcccca
      841 gcttctccgc acgcgcccag attcttgccg ggataaaaac tgaatgccgc cgctaagcct
      901 atcgagccgg ccttttgcca tctgttttct tttttcgaga aatactcggc tccgtgcgcc
      961 tggcaagcat cctcgatgac taccaggttg tacttggccg ccagctccat gatcggatcc
     1021 atatcggcgg tttgtccgta gaggtgtacc ggcacgatag ccgctacacg aagcccgcgc
     1081 tttcgctcag taagcttctt cgtttt
//
LOCUS       DATCBB010000982         1167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_290039, whole genome shotgun sequence.
ACCESSION   DATCBB010000982 DATCBB010000000
VERSION     DATCBB010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290039"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /gene="menC"
                     /locus_tag="VII37_10785"
     CDS             <1..818
                     /gene="menC"
                     /locus_tag="VII37_10785"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEY5070271.1"
                     /translation="MAKASLEAALWDAEAQQKNIPLWKLLGGTQQEISSGVSIGLKES
                     LEVLVEAVKKEIAAGYQRIKIKIKPGKDLELVRRLRQDFPRIKLMVDANSAYSMDDWP
                     LLKQLEGFYLMMIEQPLGWDDLYSHIELQKKLDTPICLDECIHNEDQAQAAVSLGACK
                     IINIKLGRVGGFTVAKRIHDICERAGVPVWCGGMLETGIGRAHNIALSTLPNFTLPGD
                     VTASKRYWNEDIIDPEVTVSSRGTIAVPQGPGIGFTPRRDLIEKLATRTERLT"
     gene            851..>1167
                     /locus_tag="VII37_10790"
     CDS             851..>1167
                     /locus_tag="VII37_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY5070272.1"
                     /translation="MDKLPMRRGGRAARIAVIPGDGIGKEVTPEALKVLKTVCASTGR
                     AIEFVEFDWGADRYLREGVSLPDGAVEMLRDEFDAILFGALGDPRVRTNQYAADILLG
                     LRF"
BASE COUNT          287 a          295 c          333 g          252 t
ORIGIN      
        1 acatggctaa agcatcactt gaagctgccc tgtgggacgc cgaagcgcag cagaaaaata
       61 ttccgctctg gaaattgctc ggtgggacgc agcaggagat tagttctggc gtgtcaatcg
      121 gacttaaaga atcacttgaa gtattggttg aggcggtaaa gaaagaaatt gccgcagggt
      181 atcaacgcat caagataaaa attaagccgg gcaaagatct ggaactggtt cgccggctac
      241 gccaggattt tccgcgtatt aaattgatgg tcgatgccaa ctccgcgtac tccatggacg
      301 actggccttt gttgaagcaa ctcgagggct tttacttgat gatgattgaa cagcccctgg
      361 gatgggatga cctgtacagc catatcgaat tgcagaaaaa gttggacacg ccaatctgtc
      421 ttgatgagtg catccacaac gaagatcagg cgcaggcagc cgttagcctg ggtgcctgca
      481 aaatcataaa cattaaactt ggccgcgtcg gcggttttac tgtcgccaag cgcatacacg
      541 atatttgcga acgcgccgga gtaccggtct ggtgcggtgg tatgttggaa accggcatag
      601 gcagggcaca caacatcgcg ttatccacgc tgccaaattt cacgctgccg ggcgatgtca
      661 ccgccagcaa gcgctactgg aatgaagaca ttatcgatcc tgaagtcact gtatcgtcgc
      721 gcggaacgat cgccgttcca cagggcccag gcataggctt tacgccgcgc cgtgacctga
      781 ttgaaaaact cgctacacga accgaacggc tcacgtagtt atctgccaaa gttgaggaat
      841 ggagagaacg ttggacaagc ttcccatgcg ccgtggtggc cgcgccgcga gaatcgcggt
      901 catcccaggt gacggcatcg gcaaagaagt tacgcccgaa gccctcaaag ttttaaaaac
      961 cgtttgcgcc tctaccggac gcgccatcga atttgtggag ttcgattggg gcgcggacag
     1021 gtatctacgc gaaggcgtga gcttgccgga cggcgccgtg gaaatgctgc gtgatgagtt
     1081 cgacgctatt ttgtttgggg cgctcggcga tccacgcgtg cgtactaatc agtacgccgc
     1141 cgatatcctt ctgggcctgc gatttaa
//
LOCUS       DATCBB010000983         1922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_128360, whole genome shotgun sequence.
ACCESSION   DATCBB010000983 DATCBB010000000
VERSION     DATCBB010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128360"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VII37_10795"
     CDS             <1..365
                     /locus_tag="VII37_10795"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEY5070273.1"
                     /translation="PATPAAPEPAANAQPPLDAAKASPAFENLIRMIGSNAAMVLGAY
                     ADPRSGQPVIDPEAARELIDMLDVLHEKTKGHLAPEEENLLLDLLGKLKMTYLEVNQA
                     LAAQAAKVAQQAKVGSKR"
     gene            465..>1922
                     /locus_tag="VII37_10800"
     CDS             465..>1922
                     /locus_tag="VII37_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5070274.1"
                     /translation="MSAESKLEPIKLTVLGSGTSMGVPSPGCHCRVCSSRDFHDKRLR
                     PSLLLSRGGQNVLIDTTPDFRQQALRVRMERLDAILLTHGHADHIMGFDDIRPFNIRQ
                     RAVLPVYGNAETFAVLRQAFAYVFTGKPTLSTVPVVDLHVVEGPFELLGVPIIPIPLV
                     HGDLEVLGYRLGKAAYLTDFSSLPETSAPMLHDLDDLIIDALRDIPHPMHQTVEQALA
                     LVRRLAPKRAWFTHIAHDLSHAETNQRLRDAGFPNVQLAYDGLHFAVRVDENDETQPE
                     NQQHCRVLPKRAAGVNTFASPAAWNAYYASPKRSSVLAVGNFDGLHLGHQAILRATVE
                     RALETKAVSTALTFDPPPLKILRPESAPQRISTNTQRIEWCSNVGLEAAVVMPFTVEL
                     SRLAPEDFVEQILVGELRVATILVGENFRFGHRQAGNVKLLRELGERLGFEVLIVPPV
                     VFRGEIVSSTIIRREIAEGDVSHAGRLLGRPFVLTG"
BASE COUNT          416 a          519 c          560 g          427 t
ORIGIN      
        1 accctgccac gccagcagcg ccggagccgg cggcgaatgc gcagccgccc ctggatgcag
       61 cgaaagcttc gcccgcgttc gaaaacttga ttcgcatgat tggcagcaac gccgcgatgg
      121 tgctgggcgc ctacgcagat ccgcgaagcg ggcagccggt aattgacccc gaagccgcgc
      181 gcgagttgat tgacatgctt gacgtgctgc acgaaaaaac caaggggcac ctggcgcccg
      241 aagaagaaaa tttgctgctc gatctgctcg gcaaattgaa gatgacctat ctggaagtaa
      301 atcaggcgct ggctgcgcag gccgccaagg tggcacaaca agcaaaggtc ggcagcaagc
      361 gttaatctcg acgccttggt gtcagcccga agtgcactaa tttttctgag atattttcgg
      421 gcacacattt cccaagatgg gttcgctgat ctccgctttt ccttatgtcc gctgaatcga
      481 agttagaacc aataaaactg accgtgctcg gctccggcac ctccatgggg gttccgtcgc
      541 cgggctgcca ctgccgcgtg tgcagttcga gagattttca cgacaagaga ttgcgccctt
      601 ccctgttgct atcgcgcggc ggtcaaaacg tgctgatcga caccacccca gattttcgcc
      661 agcaggcttt gcgggtgcgc atggaacggc ttgatgcgat tttgctgacg catggtcatg
      721 ccgatcacat catgggattc gatgatatcc ggccgttcaa tattcgccag cgagcggtgc
      781 tgcctgtgta tggcaacgca gaaacgtttg cggtgttgcg gcaagctttt gcgtatgtat
      841 tcactggcaa gccgacgctg agcactgtcc cggtcgtcga tctgcatgtt gtagaagggc
      901 catttgaatt gctgggcgtg ccgattattc ccatacctct tgtgcatggc gacttggaag
      961 ttttgggcta tcgcttaggc aaagcggcgt atctgaccga ttttagttct ttgcctgaga
     1021 cgtccgcgcc aatgctgcac gatctcgacg atttaattat tgatgcgctg cgcgatattc
     1081 ctcaccccat gcaccagacc gtggagcaag cgctggctct ggtgcggagg ctggcaccaa
     1141 agcgggcgtg gtttacccac atcgcacacg atttgtcaca cgcagaaacg aatcagaggt
     1201 tgcgtgatgc aggatttccc aacgtgcaac tggcctacga tggattgcat ttcgctgtcc
     1261 gcgtagacga aaacgacgag acacaacccg agaatcagca acattgtaga gttttgccaa
     1321 aacgcgcagc cggcgtgaat acgtttgcat caccggcggc gtggaatgcc tactacgctt
     1381 caccgaaacg aagcagcgtg ctagccgttg ggaatttcga cggtcttcat ctgggtcacc
     1441 aagcgatttt gcgggctacc gtcgaacgag ctctggaaac gaaggcagtg tcaacggcgc
     1501 taacttttga tccaccgcca ttaaaaatct tgcgcccgga atcggcgccg cagcgaatct
     1561 cgaccaacac gcaacgcatc gagtggtgca gcaatgtggg actggaagcg gcggtagtga
     1621 tgcccttcac tgtagagcta tcgcggctcg ctcccgaaga tttcgtggag cagatattgg
     1681 tgggcgaatt gcgcgtggca acgattttgg tcggggagaa ctttcgcttc ggtcaccgcc
     1741 aggccggcaa cgtaaaactg ctacgcgaac ttggagagcg gctgggattt gaagtactga
     1801 tcgttcctcc cgtggttttt cgtggagaga ttgtttccag cacaattatt cgtcgcgaaa
     1861 ttgcggaagg cgacgtgagc cacgcggggc ggttactggg gcgccctttc gtactgacag
     1921 ga
//
LOCUS       DATCBB010000984         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_387124, whole genome shotgun sequence.
ACCESSION   DATCBB010000984 DATCBB010000000
VERSION     DATCBB010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387124"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1168)
                     /locus_tag="VII37_10805"
     CDS             complement(62..1168)
                     /locus_tag="VII37_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5070275.1"
                     /translation="MTTFATAEGTRRYTARFPKAAEGHFRDAQRLAVSSLGIGTYLGN
                     PDEKTDRGYTAAIVEAAQGGINVIDAAINYRFQRSERSIGAALTELKTKGIARDELIL
                     CTKAGYLAPDGDMPSDPNEYFFREYIQPGVLGAKDIVGGGHCMTPRFLKNQLGRSLRN
                     MRVDCVDVFYLHNPESQLGDVPKADFLERIREAFTFLESAVVAGEIQYYGVATWNAFR
                     QEASARDSMQLAELVQVAQEIAGGRHRFRFIQLPFNLGMTEALTLGNQMVAGRKRTIT
                     EAADELDITLIASASLLQGQVARNLPGFVADAFGLENDAERALQFVRSAPGITTALVG
                     MSRPEHARANTRLLGVPTATVDQFSKLFARGESA"
     gene            complement(1244..>2099)
                     /locus_tag="VII37_10810"
     CDS             complement(1244..>2099)
                     /locus_tag="VII37_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070276.1"
                     /translation="TRHISEDSSGAPAAGSYYGPFSSRGAALAFANRALDFFKMRRCQ
                     IKIRRDPTFPGCLYSEMKMCLAPCFAGCSKEEYDAEVERFVKFLESSGESLRATFESE
                     RERASDDLDFERAAMLHKKVEKLDEVLRGQPDLARRIQALDAVILQRGAGDQVVAAYR
                     IQGGKLCEPFFLPFGEIASQPRSAEQIFRNYLDPTLDSVVATQLPLGVANGAAQPGAA
                     QTIEPLPLSKGELSEHLWLVARWFYSNPRTGEIFFREKNDWPYRRILRACSRLLAPPE
                     PPKEPTTN"
BASE COUNT          467 a          637 c          536 g          459 t
ORIGIN      
        1 attaccacct ggctcatcga acaagttccc gcgctaaaaa agtagcacca cgattgaaac
       61 ttcaagcgct ttcgccacga gcaaaaagtt tgctgaactg atccacggtc gcggttggca
      121 cacccagcaa ccgcgtattc gctcgcgcat gttccggccg tgacatgccc acgagcgcgg
      181 tggtaattcc cggtgcggaa cgcacaaatt gtagcgctct ctccgcgtca ttctccaatc
      241 cgaaggcatc agccacaaat cccggcaagt tgcgagcaac ctgtccctgc agcaaagaag
      301 cgctggcaat cagcgtaata tccaactcgt ccgcggcttc tgttatcgtg cgcttgcgtc
      361 ccgcgaccat ttgattgccg agcgtcaggg cttccgtcat tcccaggtta aaaggcaact
      421 gtatgaaccg gaatcgatgc cgcccgccgg caatttcctg cgcgacctgc accagctcag
      481 cgagttgcat ggagtcgcgg gcgctcgctt cctggcgaaa agcattccac gtcgctacgc
      541 cgtaatattg aatttccccg gcaaccaccg cggattccag aaacgtgaac gcttccctga
      601 tgcgctcgag aaaatccgcc ttgggtacgt ctccaagctg tgattcaggg ttgtgcaaat
      661 aaaacacgtc cacacagtcc acgcgcatgt tgcgcaggct ccgccccaat tggttcttca
      721 ggaagcgcgg cgtcatgcaa tgaccgccgc cgacaatgtc cttcgcgccc agcacgcccg
      781 gctgaatgta ttctcgaaag aaatattcat tcgggtcgct gggcatatcg ccgtcgggcg
      841 ccaggtaacc tgccttggtg cataaaataa gttcgtcgcg cgcgatgccc ttggttttta
      901 actctgtcag cgccgcgcca atactgcgtt cgctgcgttg aaatctataa ttgatggccg
      961 cgtcaatcac attgatgccg ccttgcgcgg cctccacgat tgcggctgtg taaccacggt
     1021 cggtcttctc gtcaggattc ccaagatatg taccaatgcc cagcgacgaa accgccagtc
     1081 tttgcgcgtc gcgaaaatgg ccttcagcag ccttgggaaa tcgtgccgtg taacggcgcg
     1141 ttccttctgc ggtggcaaat gtggtcatcg ttgtactcgc atatgtttat tctctactaa
     1201 tttgctgagc atggacgcga agtataacag gccgaacgct tcattaattc gtagtgggtt
     1261 ctttaggcgg ttcgggcgga gccagtaaac gcgaacaagc ccgcaggatc ctgcgatagg
     1321 gccagtcatt tttttcccga aagaaaatct ctccggtccg cgggttgctg taaaaccagc
     1381 gcgccaccag ccacaaatgc tcgctcaatt cgcctttgga cagcggcaat ggttcgatcg
     1441 tctgcgccgc gcccggttgc gcggctccat ttgccacacc caatggcaac tgtgtggcaa
     1501 ctaccgaatc gagcgtcgga tcgagataat ttctaaaaat ttgttcagca gagcgcggct
     1561 ggctggcaat ttccccaaat ggcagaaaaa atggctcgca aagcttcccg ccctgaatac
     1621 gatacgccgc gacaacctgg tcgcctgctc cgcgttgcaa aattacggca tccaaagcct
     1681 gaatgcgccg cgccagatcg ggttgaccgc gcaagacctc gtccagcttt tcgacttttt
     1741 tgtgcagcat tgctgcgcgc tcgaagtcca gatcatcact ggcacgctcg cgctcgcttt
     1801 caaacgtcgc gcgcagcgac tcgccactgg attccagaaa cttcacaaac cgctctacct
     1861 cagcgtcgta ttcctcttta gaacaaccgg caaagcaagg tgccagacac atcttcatct
     1921 ctgaatacaa acacccggga aaggtaggat cgcggcggat ttttatctgg cagcgtcgca
     1981 tcttgaaaaa gtccagcgcg cgattcgcaa aggccagtgc ggcgccgcgc gaagaaaatg
     2041 gcccgtaata acttccggcg gccggcgctc ccgaactatc ctccgaaatg tggcgcgtc
//
LOCUS       DATCBB010000985         2746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_420665, whole genome shotgun sequence.
ACCESSION   DATCBB010000985 DATCBB010000000
VERSION     DATCBB010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420665"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VII37_10815"
     CDS             <1..258
                     /locus_tag="VII37_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY5070277.1"
                     /translation="KPEVLRARIEKKASREESEISPEYIEEVARAYEHFFFHYAASDL
                     LVINTSEIDFVERSEDLQQLLRRLQDPVKGTQYFLPLGASE"
     gene            376..1569
                     /locus_tag="VII37_10820"
     CDS             376..1569
                     /locus_tag="VII37_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEY5070278.1"
                     /translation="MELWSRRKFFLTSLASSVAASANKLFARDTNPGASATPLLADAQ
                     NATQGKRPLIISSANGLHALDRGIDILKKGGDTLDAVVAAVTVVEDDPNDDSVGYGGL
                     PNEEGEVELDASCMHGPTGRAGSVASVRRIKNVSRLAKTVMERTNHVMIVGEGAHRFA
                     LDEGFEDLNLLTERSRLAWLAWKASTSFNWRPGIDSPEYVQKASIDGYNQQIAAVFDT
                     PEKRAWLPHIQNVIAHPPTGTIPCMAVDAKGDISATTTTSGLAWKIPGRVGDSPIIGA
                     GCFVDNEVGAAGSTGKGEENIKISGGHTIVEMMRRGKSPSEACLEAMSRVAHNYKKDK
                     KKLATFHIFFYAINKDGMHGAASLWRSGYDKDKHATYAVHDGTQSRLVETVPFLDEVP
                     PGEQL"
     gene            1615..>2746
                     /locus_tag="VII37_10825"
     CDS             1615..>2746
                     /locus_tag="VII37_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5070279.1"
                     /translation="MDEVNILIIGGGVVGCAIAAELSKQWQDVFVVERFPRVGMATST
                     RNSGVIHSGIYYSPGSLKARHCLQGNPLTFAFCEEHRVPHRRTGKLVVAAEAREESAL
                     EALFKRGQENGVEDLRLIGPAEIREREPHIAGVTALDVPSTGIVSAEELVRTYARVAV
                     ERNAHIVTRAQVVSLEPTAKTIRVGIRVGEDLSTQKKTSNTQVGQGEIHAQETIEARC
                     VINAAGLYADDVAALIGNHSWKIYPVRGEYCEVRGPRRSLINGLVYPLPHADGLSLGM
                     HFTKTIWDTVLIGPTARYVKDKDDYERDRFTVADFAEGARKLVPEIIDDDLQLAYSGL
                     RPKLVPPDEHGMADFVIERDGRVPQMIQLVGMESPGLTAAPSV"
BASE COUNT          659 a          767 c          760 g          560 t
ORIGIN      
        1 aaaccggaag ttttgcgcgc ccgcatcgag aaaaaagctt cccgcgagga aagcgaaatt
       61 tcccccgaat atatcgaaga agttgcgcgg gcctacgaac acttcttctt tcattacgcc
      121 gcgtccgatt tgctggtaat caatacttcg gagatcgatt ttgtggagcg ctctgaagat
      181 ttgcagcaat tgctgcgccg ccttcaggac cccgtcaagg gcacacaata ttttctgccg
      241 ctgggcgcct ccgaatagtc ctccgctgtt agaaacttaa aacttttaat tcacctaact
      301 gcctcgctgc cacatactat gcccgcgcgg ttttgcttaa gacaaaactg atatagcgaa
      361 acaacggagg ctttcatgga actatggtcg aggcgaaaat tcttcctcac ctctctcgcg
      421 agcagcgtgg cagccagcgc caacaaactt tttgcccgcg acacaaaccc aggagcctcg
      481 gcgactccat tactggccga cgcccaaaac gccactcaag gcaagcgccc gctgattatt
      541 tccagcgcca atggcctcca cgccctcgac cgcggaatag acatcctcaa aaaaggcggc
      601 gacaccctgg acgccgtcgt ggctgcggta accgtggtgg aagatgaccc caacgatgac
      661 tccgtcggct acggaggcct tccaaacgaa gagggcgaag tggagctaga cgccagctgc
      721 atgcacggcc cgacgggacg tgcgggctcc gtggccagcg tgcgccgcat taaaaatgtt
      781 tcgcgtctgg caaaaaccgt gatggagcgc accaatcacg tgatgattgt cggcgaagga
      841 gcgcaccgtt tcgcgctcga cgagggcttc gaagatttga atctcctcac cgagcgttcc
      901 cggcttgcgt ggttagcgtg gaaagcttcc acgtcgttca actggcgccc gggaatcgac
      961 agtcccgaat acgttcagaa agcctcgata gatggatata accagcaaat agccgccgta
     1021 ttcgatacgc ctgaaaagcg tgcatggctg ccgcacattc aaaacgtgat tgcacatcca
     1081 cccacgggca cgattccttg catggcggtc gatgctaaag gagacatttc cgcaaccacc
     1141 accacctcgg gactggcgtg gaaaattccc ggccgcgtag gagattcacc gatcatcggc
     1201 gcaggatgtt ttgtggacaa cgaagtcggt gccgcgggct cgacgggtaa aggcgaagag
     1261 aacatcaaaa tttctggcgg gcacactatc gtggaaatga tgcgccgcgg caagtctccc
     1321 tccgaggcgt gcctcgaagc tatgagccgc gtggcccaca actacaaaaa agataagaaa
     1381 aagctggcca cctttcatat atttttttat gccataaata aagacggcat gcacggagcc
     1441 gcttcgctgt ggcgctctgg ttatgacaaa gacaaacacg caacttacgc cgtgcatgac
     1501 gggacacagt cgcggttggt ggaaacggta ccttttctcg acgaggttcc gccaggcgag
     1561 cagttgtaag cataaaatta ttctggctgc tcttatcgga ctaacttcac cctcatggac
     1621 gaagtaaata ttttaattat cggcggcggg gtagttggct gcgccatagc cgcggaactt
     1681 tccaaacaat ggcaggatgt cttcgtcgtg gaacgctttc cccgcgtggg aatggctacc
     1741 agcacgcgca atagtggtgt catccactcg ggaatttact attcgcccgg ctcgctcaaa
     1801 gcgcgccatt gcctgcaagg caatccactc acatttgctt tctgcgaaga gcaccgtgtc
     1861 ccgcaccggc gcaccggcaa gttggtcgta gccgctgaag cacgcgaaga atccgcattg
     1921 gaagcgttat ttaagcgagg ccaggagaac ggcgtggaag acttgcgatt gattggcccc
     1981 gcggaaattc gcgagcgcga accacacatt gctggggtta cggccttgga tgtccccagc
     2041 acgggcatcg tttcggcgga agaattagtg cgtacttacg cgcgtgttgc agtggagcga
     2101 aatgcgcaca tcgtcactcg tgcgcaggtg gtgtcactgg aaccaaccgc gaagaccatc
     2161 cgcgttggga ttcgcgttgg cgaagattta agcacgcaga agaaaacctc aaacacgcag
     2221 gtggggcaag gcgaaatcca tgcacaggaa accatcgaag cgcgctgcgt cataaacgcc
     2281 gccggattat atgccgacga tgtcgccgcg ctaatcggca accattcgtg gaaaatttat
     2341 cccgtgcgtg gggagtactg cgaggtgcgc ggcccgcggc gttccttgat caacgggctg
     2401 gtttatcccc tgccccatgc cgatggactc agcctcggaa tgcatttcac aaaaacaatc
     2461 tgggacacgg tattaatcgg gccgaccgcg cgatacgtga aagataaaga tgattacgag
     2521 cgagatcgct tcaccgttgc cgattttgcg gagggagcca ggaaattggt gcccgaaatt
     2581 attgacgatg acttgcaact cgcttactcg ggcttgcggc caaaactcgt gccgcctgat
     2641 gaacacggca tggccgactt tgtgatcgaa cgcgacggca gagtgccaca gatgattcaa
     2701 cttgtgggga tggagtcgcc cggcctgacc gctgcgcctt ccgtgg
//
LOCUS       DATCBB010000986         1172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_128427, whole genome shotgun sequence.
ACCESSION   DATCBB010000986 DATCBB010000000
VERSION     DATCBB010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128427"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1172
                     /locus_tag="VII37_10830"
     CDS             <1..>1172
                     /locus_tag="VII37_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY5070280.1"
                     /translation="PSNGQRVQLFLRRHPDEFYIPTIAVLCCMIMSAIVLLLTDPFNS
                     PGLVFLSLLALLLPSSQSAVELVNYLTTSLLRAEILPKLDFSDGIPGDCVTLVAVPTL
                     LLNEKQVRRLVDDLEVRFLGNHDRNLHFVLLSDLPDSRQPSREDDPLIDLCAELIERL
                     NEKYAAQGSGSFLLLHRHRVYNPREGVWMGWERKRGKLLDLNKLLRQQYDSFPVKAGN
                     LAVLPEARYVITLDSDTELPRGSAHRMVGTLAHPLNQAMIDSENNIVVAGYGILQPRV
                     GVSVQSAASSRLANIYSGQTGFDIYTRAVSDVYQDLYGEGSFAGKGIYEVDTVHQVLD
                     RRFPRNALLSHDLIEGAYARAGLVSDIEVIEDYPSHYSAHNRRKHRWLRGDWQITA"
BASE COUNT          237 a          314 c          337 g          284 t
ORIGIN      
        1 gaccgtcgaa tggccaacgt gtgcaacttt ttctgcggcg gcatcccgac gagttttaca
       61 tccccactat tgccgtcctg tgctgcatga ttatgtcggc gattgttttg ttgctgaccg
      121 atccattcaa ttcccccgga ttggtctttt tgtcgcttct ggcactgttg ctgccgtcgt
      181 cacagagcgc ggtggaactg gtgaactatc tgaccacgtc actgttgcgc gcggagattc
      241 tgcctaagct ggatttttcg gacggcattc ccggagattg cgtcaccctg gttgcggtgc
      301 ccactttatt gctgaacgag aaacaggtcc gaagactggt tgacgatctt gaagtgcggt
      361 tcctgggaaa tcacgaccgc aacctgcact ttgttttgct gagcgatttg ccggattcgc
      421 gccagccctc gcgcgaggac gatcctctca ttgacctctg cgccgaactc atcgagcgat
      481 tgaacgagaa atacgccgcc caaggcagcg gctcgttcct gcttcttcat cgccaccgag
      541 tctacaatcc gcgcgaaggc gtttggatgg gctgggagcg gaagcgcggc aaactgcttg
      601 accttaataa gctgcttcga cagcagtacg acagtttccc ggtgaaggcg gggaacctgg
      661 ctgtgcttcc tgaagcgcgt tacgttatta ctctcgattc cgatactgag cttcctcgcg
      721 ggtcggcgca ccgcatggtt ggaaccctgg cgcatccgct gaaccaggca atgattgact
      781 ctgaaaacaa tattgttgtc gcagggtatg ggattctgca gccacgagtg ggagtaagtg
      841 tgcagagcgc cgcgtcatcg cgactggcga atatctattc tggccagacg ggattcgata
      901 tttatacccg cgcggtttcg gacgtgtatc aggatttata cggggaagga agttttgcgg
      961 gcaaaggaat ttatgaagtg gacacggtac atcaggtgct tgaccggcgt tttccgcgca
     1021 acgccttgct tagtcacgat ctcattgaag gcgcgtacgc aagagcgggc ctggtaagcg
     1081 atatcgaggt catcgaagac tacccgtcgc actacagcgc tcacaatcgc cgcaaacacc
     1141 gctggttgcg cggagactgg cagattacgg cg
//
LOCUS       DATCBB010000987         1982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_322513, whole genome shotgun sequence.
ACCESSION   DATCBB010000987 DATCBB010000000
VERSION     DATCBB010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322513"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VII37_10835"
     CDS             <1..471
                     /locus_tag="VII37_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070281.1"
                     /translation="GTLICFDGSCGPLIKQWKLPLRNVLEGLRSTDFYCPGSILGINV
                     DTSNPIARTMSKDVNAYFINSSAFEITNTPKGTFDAPQTPWPVQAIARYAKDDVLRSG
                     WLRGEDKIKNRIALAEVRMGKGRIVLFAFRPQHRGQTWGTFPFIWNAINLAQKQ"
     gene            912..>1982
                     /locus_tag="VII37_10840"
     CDS             912..>1982
                     /locus_tag="VII37_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5070282.1"
                     /translation="MKRIITLVLLCSFAVALAQAAGAQSRPRRVGASPPPQQQDQQTQ
                     PQQPSTQSQTQANDGSQTTQPSRPPVLGGANRDPNEQKPESTKSKDAGPEEVSAGDIV
                     RVDTTLVSLPVSVMDRDGKYIPNLGKEDFRVWEDGVEQRVAYFASTEKPFTVALVIDT
                     SASTRYKLEEIQDAAIAFVNQLRPDDRVMVVSFDDKIRVLEQPTSDRSALRNAILQTR
                     IGSGTRLYDAMDQVINQYFNRIEGRKAIVLFTDGVDTTSKHASYESTVRDAEELDALI
                     YPVEYDTSSDMAGWPGGGGSRRGGGGGGGGGNGSVVIDILGAILGGGSNNGGGGGNSR
                     GGGGGSNPGGGGGRRGGGGGSGN"
BASE COUNT          433 a          540 c          592 g          417 t
ORIGIN      
        1 ggcacgctga tttgttttga tggttcatgc gggccgttga tcaagcagtg gaagttgccc
       61 ttgcggaacg ttttggaagg cctgcgctcg accgattttt attgtcctgg ttcgatcctg
      121 ggcatcaacg tggacacctc aaatccgatc gcgcggacca tgtcgaaaga cgtcaatgct
      181 tacttcatca acagctcggc attcgagatt actaatactc ccaagggcac attcgacgcc
      241 ccgcaaactc cttggccggt gcaagcgatt gcgcgttacg cgaaagatga tgtgctgcgt
      301 tcgggttggt tgcggggtga agacaagatc aaaaatcgga ttgcgctggc tgaagtcaga
      361 atgggtaaag gccgcatcgt tctcttcgct tttcgccccc agcatcgcgg ccagacctgg
      421 ggcacgtttc cattcatctg gaacgcgata aatttggccc agaagcaata gccagtttga
      481 gcggccaggc taccgactgc tgggagcgcg gacgtctcgt ccgccctgag cgcaaagcgc
      541 gaaaaagaat gttggccaca agctactgat attttccaga ccacttcgcg cctgcggcgc
      601 ggtgcgggcg ggacgcccgc gctcccagca cgaggttgac tgggtcagac atccctgaat
      661 tccgaccatc acgatttctt gcttaagcga gagcccagtg ctatcttcgc catgtcagtt
      721 tggaatctct ccccattttc tctcccgaaa aaaccggagc accggcgcca ctgagtgtgc
      781 ttttctgagc atctctttgc tgataaccgc atcaaaagaa gcggtgtgct tgagaaacca
      841 ggcgcggtgc gcgatctcaa ttttatgcgc cagaaaccgg ccaacgctct tcccgacagg
      901 agttttacgt gatgaagaga attattaccc tcgtcctgct ttgttccttc gcggtagcgt
      961 tagcgcaagc tgctggtgcc cagtcacgac ctcgccgagt aggcgcgagt ccgccacccc
     1021 agcaacaaga tcagcagacg caacctcagc agccttcaac gcagtcgcag acacaagcga
     1081 atgatggttc ccaaacgacg cagccgtcgc gcccgccggt gctgggcgga gcgaaccgcg
     1141 atcccaacga gcaaaagccg gaatcgacta aatcgaaaga cgccggccct gaggaggtta
     1201 gtgcgggcga tatcgtgcgc gttgacacga ccctggtcag tcttccggtc agcgtaatgg
     1261 atcgcgacgg caagtacatt cccaatctgg gcaaagaaga ttttcgcgtg tgggaagatg
     1321 gcgtggaaca gcgagtggcg tatttcgctt cgaccgaaaa accgttcacg gtagccctgg
     1381 tgatcgacac cagcgcttcg acgcgttaca agttggaaga gattcaggat gccgcgatcg
     1441 ctttcgtcaa ccaactccgc cccgatgatc gcgtgatggt ggtgtcgttc gacgataaga
     1501 ttcgagtcct ggaacaacca accagcgatc ggtcggcgct ccgcaatgcg attcttcaga
     1561 ctagaatcgg ttccggtact cggctttacg acgcaatgga tcaggtgatt aatcaatact
     1621 tcaatcgcat cgaagggcga aaggccatcg tgttgttcac tgacggagtt gatacgacca
     1681 gcaagcatgc gagttacgaa agcactgtgc gcgacgccga agagctggac gcgctgatct
     1741 atccggttga atacgacacc tcttccgaca tggctggatg gcctggcgga ggcggttcgc
     1801 gtcgcggcgg cggcggtggt ggtggcggtg gcaatggcag cgtcgttatc gatatccttg
     1861 gcgcgattct cggaggtgga agcaacaacg gtggcggagg cggtaattcc cggggtggcg
     1921 gcggaggcag taatccgggc ggtggaggcg gacgacgcgg tggtggcggt ggttcaggta
     1981 ac
//
LOCUS       DATCBB010000988         1041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_192533, whole genome shotgun sequence.
ACCESSION   DATCBB010000988 DATCBB010000000
VERSION     DATCBB010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192533"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..>1041)
                     /locus_tag="VII37_10845"
     CDS             complement(162..>1041)
                     /locus_tag="VII37_10845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5070283.1"
                     /translation="VVGAVGRPPEMTAEFCATDLKAAPGSQVVLLLALMEKAEPDVLG
                     GLMVESSDGSILGELGLESALGVMITDKETQAPAGMLIVGRAKAHKWRPNEVYFLQAI
                     GDQMLMSVSHTRLRSLVRRMGVSDERTGLLSRSSYQTCLLTEADRAKTQGTPLALAIL
                     QIDRGAEILRQQGESPMERFLEQLARSLQSLLRQNDVVVKYTSWSLAFVLPDTTLDGA
                     QNLAEKLLRAATGLRPPWDATQITLSAGIVEATAKPDYDSEDIVTDLINRAEFSIEEA
                     KKRGGDTVIVLDSPRV"
BASE COUNT          211 a          395 c          233 g          202 t
ORIGIN      
        1 gaccgccgaa tcgtttcttt cgacgcatgc accgccagat cgatggccga cacatccgaa
       61 aaagatcccg tgtagcgcga catcggtgtg cgcgcccccg caacaatcac cacatccgtt
      121 ggaaacataa aatcctctct gctccgcact ccaagaaact tctaaactcg gggactatcc
      181 agcacgatca ccgtatcccc gccccgcttc ttcgcttcct caatcgaaaa ttccgcccga
      241 ttgatcaagt ccgtaacaat atcctcgctg tcgtaatccg gtttggccgt ggcttccaca
      301 attcccgcgc tcaaagtgat ttgtgtcgcg tcccacggcg gccgcagacc cgtcgccgcc
      361 cgcaacaatt tctccgccag gttctgcgct ccatccagcg tagtgtccgg caaaacaaac
      421 gccagcgacc acgacgtata cttcaccact acatcgttct gccgcagcag cgattgcagc
      481 gagcgcgcta gctgctccag gaatcgctcc atcggcgatt cgccctgctg ccgcaaaatt
      541 tccgccccgc gatcgatctg caaaatcgcc agcgccagcg gcgtcccctg cgtcttcgcg
      601 cgatccgctt ccgtcagcag acaagtctga taagagctgc ggctcagcaa tcccgtgcgc
      661 tcatcggaaa cgcccatgcg ccgcaccagc gagcgcagcc tcgtgtggct cacgctcatc
      721 agcatctggt ccccgatggc ctgcaaaaaa taaacttcgt tcggccgcca tttgtgcgct
      781 ttcgcgcgcc ccacgatcag catccccgcg ggagcctgcg tctccttgtc cgtaatcatc
      841 acgcccagcg cgctctccaa acccaattcg cccagtatcg acccgtccga cgattccacc
      901 atcagtccgc ccagcacatc cggctccgct ttttccatca gcgccagcaa cagcaccacc
      961 tgcgatccgg gagccgcttt caaatccgtc gcgcaaaatt ccgcggtcat ttccggtggc
     1021 cgccccaccg cgcccaccac c
//
LOCUS       DATCBB010000989         1718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_192565, whole genome shotgun sequence.
ACCESSION   DATCBB010000989 DATCBB010000000
VERSION     DATCBB010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192565"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..678
                     /locus_tag="VII37_10850"
     CDS             67..678
                     /locus_tag="VII37_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY5070284.1"
                     /translation="MSGRKPPSSTRGRQASKANRNGPGTAFSESRIAERVAELGKEIS
                     RAYEGRRLDVVITLDRGFVFGADLIRELKGPVVCHFVREDLRDVEDSGRPRREVLFGS
                     APNLKGRDVLLVDAVLESGVTQDFLLRRLGESSPRSLRLAVLLDKHEKRRVDLESDYF
                     GFRTASKEVWIGYGLAAANGTGRNLRQLSNGAKVTEKTERRKK"
     gene            716..>1718
                     /locus_tag="VII37_10855"
     CDS             716..>1718
                     /locus_tag="VII37_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY5070285.1"
                     /translation="MNSSSTAKTILFWISIVFLGVMLWKLVSANGAQAREDEPSYSEF
                     MAKVDASDVKEVTLYLSPNSYELQGEYVRPPNHKFHLTVFKESAPDLAKQLRDKAVPM
                     KVKEVRSGDWVLILLNAAPLILLVGFCLFLMRQMQAGGNKALSFGKSRARLLSAQQKK
                     ATFKDVAGTDEAKEELQEIIEFLKDPQKFQKLGGRIPKGVLLVGPPGTGKTLLARAIA
                     GEANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGL
                     GGGHDEREQTLNALLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRRVVVARP
                     "
BASE COUNT          375 a          434 c          527 g          382 t
ORIGIN      
        1 gtccgccaaa tttgcggcca gcgatagaac caaaacgggg atcgtgatcg tcgaggaaaa
       61 gcttttatga gcggccgcaa gccgccgagt tctacacgtg gtcgccaggc gagcaaagca
      121 aatcggaacg ggccgggtac ggcattctcc gagtcccgca tcgccgaacg ggtagctgaa
      181 ttgggcaagg aaatttcgcg cgcttacgag gggcgccgcc tggacgtggt gattacgctg
      241 gaccgggggt tcgtgttcgg cgcggatttg atacgagagc tgaaaggccc ggtagtctgt
      301 cattttgtgc gcgaagattt gcgcgatgtt gaggacagcg ggcggcctcg ccgggaagtt
      361 ttgtttggct cggcaccgaa cttgaaaggc cgcgatgttc tgttggttga cgcggttctc
      421 gagagcggcg tgacgcagga ttttctgctg cggcgattgg gtgagagctc accgcgctcg
      481 ttacggttgg cggttttgct ggacaagcat gaaaagcgcc gcgtagatct tgagtcggat
      541 tactttggct tcagaactgc atctaaagaa gtatggatag gatatgggct ggcggcggct
      601 aacggcacgg gtcgcaacct tcgccaactg tcaaacggcg caaaggtcac agagaagact
      661 gagcgtcgaa agaaataaag ttgctgcaaa ggctgcgtag attagtgagg gcaacgtgaa
      721 ttccagttcc actgcaaaga ccattctgtt ttggatttcg attgtatttc tgggtgtgat
      781 gctgtggaag ctggtgtccg ccaacggcgc gcaggcgcgc gaagacgagc ccagttacag
      841 cgagttcatg gccaaggtcg atgccagcga cgttaaagaa gtcacgctgt acctctcacc
      901 caatagttac gagctgcaag gtgagtatgt ccgaccaccc aatcacaaat ttcatctaac
      961 cgttttcaag gaatcggcac cggacctggc gaaacagttg cgcgacaaag ccgtgccgat
     1021 gaaagtcaaa gaagttcgca gcggcgactg ggtgctcatt ctgttgaatg ctgctccgct
     1081 cattttgctg gtgggattct gtcttttctt gatgcggcag atgcaagcgg gcggcaacaa
     1141 ggctctcagc ttcggcaaat cccgcgcgcg tttgctttcc gctcagcaga aaaaagctac
     1201 gtttaaggat gtggcgggaa ccgacgaagc caaagaagaa ctgcaggaga ttattgagtt
     1261 cctcaaggac ccgcaaaaat tccagaaact gggtggccgc attcccaagg gcgtgctgct
     1321 cgtcgggcct cctggaacag gtaagacttt acttgctcgc gcgatcgccg gcgaagctaa
     1381 cgttcccttt ttctccatct cgggttcgga cttcgtggaa atgtttgtgg gcgtgggcgc
     1441 cagccgcgtt cgtgacctat tcgagcaggg caagaagaat gctccctgca tcatcttcat
     1501 cgatgaaatt gacgccgtcg gccgccatcg tggcgccgga ctgggcggcg gtcatgacga
     1561 gcgcgaacaa actctcaatg ccctgctcgt tgaaatggac ggtttcgaat ccaacgaagg
     1621 cgtgatctta atcgccgcaa ccaatcgtcc tgacgtcctt gatcctgcgt tgctgcgacc
     1681 tggccgtttc gatcgtcgtg tagtggtggc ccgtcctg
//
LOCUS       DATCBB010000990         1248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_192567, whole genome shotgun sequence.
ACCESSION   DATCBB010000990 DATCBB010000000
VERSION     DATCBB010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192567"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..927)
                     /locus_tag="VII37_10860"
     CDS             complement(103..927)
                     /locus_tag="VII37_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5070286.1"
                     /translation="MIEEVGLDDYSTKRLRQSPDGFKHIKFTVEGSVARLTLNRPEHN
                     LLNEAMLRELSDGVSVAGDREDIKLIVLDSACKVFCGGIDIGEYTSQRVFQMLDAFHA
                     AFSGMLEVGKPVICVVNGPAIGGGAELAAFGDFVIATPKARFAQPEISIGVFPPLAST
                     ILPFLVGPKVALELVLTGEPVTAERALELGLINRLVPETQLQAAVSDLIGRITSHSGP
                     VLTMAKKAILGGMGMSLKDGLKHSMNIFLNELYRLEDSQEGLRAVVEKRKPNWKNR"
     gene            1025..>1248
                     /locus_tag="VII37_10865"
     CDS             1025..>1248
                     /locus_tag="VII37_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070287.1"
                     /translation="MRKRFAPPSRAIHDTVLAAHFHGAVDAAGFPLPKAWESAKPIHF
                     DTDWQGKNPDPHRQTQVRLLWTPQFLHLKFV"
BASE COUNT          302 a          406 c          263 g          277 t
ORIGIN      
        1 tacaaattga aaattgagtg tatgtcgcgc cccccagcgt gtcaacgcgt gaacaaacca
       61 cgctcctcga aattttacac gccacacctt tttgacgatc gctcagcgat tcttccagtt
      121 tggtttgcgt ttttctacca ccgcgcgcaa cccctcctgt gaatcctcca accggtacaa
      181 ttcattcagg aaaatattca tggaatgttt caagccatct ttcagtgaca tccccatccc
      241 accaaggatc gcttttttgg ccatcgttaa taccggcccg gaatggctgg tgatgcgccc
      301 tatcaaatca gacaccgctg cctgcaattg cgtttccggc accagccgat tgatcaatcc
      361 caattccaac gcgcgttccg cagtcaccgg ctcgccggtc aaaaccagtt ccaacgccac
      421 cttcggtccc actaaaaaag gcagaatcgt tgaagccagc ggcggaaaga ctccaatcga
      481 gatttccggt tgcgcaaacc gcgccttcgg cgtggcaatc acaaaatcac caaatgctgc
      541 aagctccgcg cctccgccga tcgccgggcc gttcaccacg cagatcaccg gcttgcctac
      601 ttccagcatc ccggaaaacg ccgcatgaaa tgcatccaac atctgaaaca cgcgctgcga
      661 ggtatattct ccaatatcaa ttccgccgca aaatactttg cacgcggagt ccagcacaat
      721 cagttttatg tcttcgcgat cgcccgccac actcacacca tcggaaagct cgcgcagcat
      781 cgcttcgttc aggagattgt gctccgggcg gttcaacgta agccgcgcca cgctcccttc
      841 cacagtgaac tttatgtgct tgaacccgtc ggggctctgt ctcaatcgct ttgtcgaata
      901 atcatccagt ccgacttcct cgatcatagg tgctccagat actccagaaa tttcgccaag
      961 ttacacaacg aacaaccgat gctagcatgg ccattccgcg cttttaggag acaaaaacta
     1021 gcccgtgcgc aaaaggttcg ccccgccctc cagagcaata cacgacacgg ttctcgccgc
     1081 tcatttccat ggcgcggtcg atgccgccgg tttcccgctt ccaaaagcgt gggaaagcgc
     1141 aaagcccatc cacttcgata ccgactggca agggaaaaat cctgatccac atcgtcagac
     1201 ccaagttcgc ctgctgtgga ccccacaatt tctgcacctt aaatttgt
//
LOCUS       DATCBB010000991         1303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_161582, whole genome shotgun sequence.
ACCESSION   DATCBB010000991 DATCBB010000000
VERSION     DATCBB010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161582"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VII37_10870"
     CDS             <1..197
                     /locus_tag="VII37_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070288.1"
                     /translation="AINSSLSATVADSIPHWAGGLPPDVPPRPSDPKYADYLAKLKGK
                     AVVDAPKADVVEEIKPHSIY"
     gene            241..450
                     /locus_tag="VII37_10875"
     CDS             241..450
                     /locus_tag="VII37_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070289.1"
                     /translation="MRTVIAAIAITLLAVTAPAQAIAKGKRQQQDAPKAEDQVKKKAA
                     EEAYKSALKSIPASNEKPDPWKTMR"
     gene            740..1150
                     /locus_tag="VII37_10880"
     CDS             740..1150
                     /locus_tag="VII37_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070290.1"
                     /translation="MAIAPVSTTSASIPPPIDPMRQAFGQLTGAIQSGDLSAAQSAFA
                     ALTQAQPNQGSGPFSQALSQIGDALQSGDIGKAQQALAALQQQMQAMKGAHHHHGHHH
                     GGGGDKSQSASASPSTSSDPTTSTTSTNLVDVTA"
BASE COUNT          338 a          380 c          350 g          235 t
ORIGIN      
        1 gcgctattaa ttcatcacta tccgcaactg tggcggatag cattccgcac tgggcagggg
       61 gcttgccccc ggatgtcccg ccaagaccga gcgatccgaa atatgctgat tatcttgcta
      121 aactgaaggg aaaagctgtg gtcgatgcac caaaggcgga tgttgtcgaa gagattaaac
      181 cacactcaat ttattgaaga aggcaactaa catgcagtcc aaatacggaa ggactttaaa
      241 atgagaaccg ttatagccgc gattgcaatt acattacttg cagtgaccgc tcctgcccaa
      301 gccatcgcaa aagggaaaag gcagcaacag gacgcgccaa aggcggaaga ccaagtaaag
      361 aagaaagctg ccgaggaagc atacaaaagc gcgctcaaaa gcataccggc atcgaacgaa
      421 aagccagacc cctggaaaac catgcgctag gtcgtcgctt ggccgaaagc ccgcgaaccg
      481 ccgcatgacc ggacgtgcat aaagcttcca taaaaaattg cgtaaaagcc gtaacgagat
      541 ttgatgatac gcagataccg cggcaatgca tttgtccaag catcgcgcaa ctttttcacg
      601 cacgggctcg gcacgccggg cgtaaacgcg ggcagcatcg aattttttat taaccgtttg
      661 caaatcaccg cctgatagca ccgtgagcac gggcgacgcg attccatgca ggacatgacg
      721 gtcctgccgg agacgtgtca tggcaattgc tccagtttcg accacctctg ccagtattcc
      781 gccgccaatc gatccgatgc ggcaggcgtt cggacaactg accggcgcaa ttcagtccgg
      841 cgacctctcg gccgcgcaaa gcgcctttgc ggcgctcacg caggcgcagc ccaatcaagg
      901 tagcggtccg ttttcccagg cgctgagcca gatcggcgat gccttgcaat ccggcgacat
      961 cggcaaagca cagcaggcgc tcgcggcatt gcagcagcag atgcaggcca tgaagggtgc
     1021 gcatcaccat catggccatc atcacggcgg tggcggcgat aaatcgcaat ccgcaagcgc
     1081 atcgcccagc accagcagcg atccgacgac gtcaacgacg tccacgaacc tggtcgacgt
     1141 gacggcttaa cgcgcaatcg cgcaacgttt ccattgaagg tatttccggc gcaagcctaa
     1201 gcgtctgcgg gctcgcatgt tcgataccat cgtcatgctc accggacctg tccctgctca
     1261 aaagcctatc cggtctggga atcggtttgg cgatcgcgac tga
//
LOCUS       DATCBB010000992         1129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_192636, whole genome shotgun sequence.
ACCESSION   DATCBB010000992 DATCBB010000000
VERSION     DATCBB010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192636"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VII37_10885"
     CDS             complement(<1..1113)
                     /locus_tag="VII37_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070291.1"
                     /translation="MPAAPYKMVDNANSLLDVSDLVFIDMPGTGFGRLNGKDAEKAFW
                     GIDEDANAFARFIARFITKYNRWNSPKYIFGESYGTTRSAVLADILENSKNIDINGVI
                     LLSQIFNFTTDIDIPMGNPGVDLPYVLALPTYAATAWYHKKLPAQPAALEPFLKDVEE
                     FTNGPYAHALALGTDLGAAEKETVAEKLHEYTGLPVAYLLKANLRVRGGEFEKTLQDD
                     QDTTTGRLDTRFSGPTLDPLSQFADYDPQSSAISSAYVSLFNDYVRRDLKYGEGQTYL
                     PEAQFGIAQWDFKHRGNPVSVNVAPDLAEAMKTNPRLKVMVNGGYYDLATPFFAAQYE
                     EKHLPIPQSLAKNIEYDWYESGHMVYVRDESLKQLHD"
BASE COUNT          228 a          386 c          267 g          248 t
ORIGIN      
        1 atcatgcaat tgcttcaagc tctcgtcgcg cacataaacc atgtgccccg actcatacca
       61 gtcatactcg atattcttcg ccagcgactg cggaatcggc agatgctttt cctcgtattg
      121 cgcggcaaaa aatggcgtag ccaaatcgta gtagccgcca ttcaccatta ctttcagtcg
      181 cggattggtc ttcatcgcct cagccaaatc cggagccacg ttcacgctta ccggattgcc
      241 ccgatgcttg aagtcccatt gcgcgatccc gaactgcgct tccggcaaat aggtctgtcc
      301 ctccccatac ttcagatcgc gccgcacgta gtcgttaaat agcgaaacat acgccgacga
      361 aatcgccgag ctctgcggat cgtaatccgc aaactggctc agcgggtcga gcgttggccc
      421 ggagaagcgc gtatccagcc gcccggtcgt ggtgtcctga tcgtcctgca gtgtcttctc
      481 gaactccccg cccctcaccc gaagattggc cttcagcagg taagccaccg gcagccccgt
      541 atactcatgc aacttctctg ccacggtctc cttctccgcc gcgcccaggt ccgtccccag
      601 cgccagcgca tgcgcgtaag gcccgtttgt aaactcctcc acatccttca ggaacggctc
      661 cagcgccgcc ggctgcgcag gcagcttctt gtggtaccac gccgttgccg cgtacgtcgg
      721 caacgccagc acgtacggca aatccacccc cgggttcccc atgggaatat cgatgtccgt
      781 cgtaaagttg aaaatctgcg acagcagaat cacgccgttg atgtcgatat tcttgctgtt
      841 ctccagaatg tcggccagca ccgccgaccg cgtcgtacca tagctctccc caaaaatgta
      901 cttgggcgaa ttccaacggt tgtacttcgt aatgaaccgc gcaatgaaac gcgcaaacgc
      961 attcgcgtct tcatcgatcc cccagaacgc cttttccgca tccttcccgt ttagccgccc
     1021 gaatcccgtt cccggcatgt cgatgaacac caggtcgctc acatccagca gcgaattcgc
     1081 attgtccacc atcttgtatg gcgccgccgg catgtgcgtg tccgtgtcc
//
LOCUS       DATCBB010000993         1992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_290526, whole genome shotgun sequence.
ACCESSION   DATCBB010000993 DATCBB010000000
VERSION     DATCBB010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290526"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..965)
                     /locus_tag="VII37_10890"
     CDS             complement(186..965)
                     /locus_tag="VII37_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5070292.1"
                     /translation="MRYYPLCQEAKALVASGAIGNPRLVHGSFLQDWLLHASDWNWRL
                     VPKLGGELRAVSDIGTHWLDLIMWITGAKVNEVCADLGTTVPVRQRPRGRVETFKTRA
                     TNEFDEVPITTDDYASVLLHFENDMRGAMTVSQVSAGRKARLWFEIDGTEGSLAWDSE
                     SPNELWIGRRDEANGLLCKDPALLSPEARGYAAYPGGHAEGYPDTFVQLFRDFYEYVD
                     TGDFEAPQKFPNFLTGHQELTLCEAIAKSAQKRTWVEALLE"
     gene            complement(999..1331)
                     /locus_tag="VII37_10895"
     CDS             complement(999..1331)
                     /locus_tag="VII37_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5070293.1"
                     /translation="MKAAAILIGAGFVGKAHVDALRRLGIEVRGVLGSSPERSEEARA
                     ALSLPRAYVSIEEAAADNTASVAHVCTPNYLHFEQSSTLLRAGKHVLCEKPLATDTAE
                     SEILVRLA"
     gene            complement(1395..>1992)
                     /locus_tag="VII37_10900"
     CDS             complement(1395..>1992)
                     /locus_tag="VII37_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070294.1"
                     /translation="LVTVKRAGTPMMARAGDESRVLFKAAVNDEFEFLDADGEWVHVQ
                     ISGVSRGYIRKSALELPEVIAERLEATAEAKSAAKPAAFRLEREDNSVFPGGWEALKG
                     KTVKIYTVQPASQDPKDTDARAKLNFAGSLMQKFVEDVTPGAPSVDGVVVIFDSADGG
                     LTGATLASIQQLAKGEISEEAFWRQCYLDPPETFQPKR"
BASE COUNT          420 a          639 c          489 g          444 t
ORIGIN      
        1 tggcgaggat tatcccgcgg accctgggca tctcgactac caactggaat tcaatactcg
       61 ccagcgctcc gatcggttgc cgtcgtcgtt gcgctaccaa tacgcgccca acccctgaag
      121 gaaccacggg tgcaccggaa gtcagcaggc ttcggcgtcc aatagaaact aattttgaat
      181 gcgtactact ccagcagcgc ttctacccac gtgcgcttct gtgcgctctt tgcgattgct
      241 tcgcaaaggg ttaattcttg atgtcccgtc aaaaaattcg gaaacttctg cggcgcttca
      301 aaatctcctg tgtccacata ttcgtaaaaa tcacgaaata attgcacaaa cgtatccgga
      361 tatccttccg catggccgcc cggatacgct gcataaccgc gcgcttcagg actcagcagt
      421 gcaggatctt tgcacaacaa cccgttggct tcgtcccgcc ggccgatcca taactcattc
      481 ggtgattccg aatcccacgc cagcgatcct tcggtaccat cgatttcaaa ccacagcctg
      541 gctttgcgcc ccgcgctgac ctgcgaaaca gtcatggctc cgcgcatgtc gttctcgaaa
      601 tgcaacaaaa ccgatgcgta gtcgtcggtg gtaatgggca cttcgtcgaa ttcgttcgtc
      661 gcgcgggttt tgaaggtctc cacacggcca cgtggacgct gccgcactgg cacggtcgtt
      721 ccaaggtccg cgcaaacttc gttaactttg gctccggtga tccacatgat caaatccagc
      781 caatgcgttc cgatatccga cacggcgcgc aattcgccac ccagtttcgg caccaatcgc
      841 caattccaat cgctggcatg cagcaaccag tcctgcaaaa aacttccgtg caccaggcga
      901 ggattcccaa tggcgccact cgccaccagc gctttggcct cctggcaaag cgggtagtaa
      961 cgcaagttgt aagctacgcc tcccgcgcgc ttacactctc aggcaagtct gaccagtatt
     1021 tccgactcgg cggtgtcggt ggccaggggc ttttcgcaca gcacgtgctt gccggcgcgc
     1081 agtaacgtgc tcgactgttc gaaatgcaaa tagttgggcg tgcagacatg cgccacactg
     1141 gcggtattgt ccgccgcggc ttcctcaatc gagacgtagg cgcgtggcag actcagcgcc
     1201 gcgcgtgctt cctcgctgcg ttccggcgaa cttcccagaa cgccgcgcac ttcaatgccg
     1261 agccgccgca gggcgtcgac atgcgctttg cccacaaaac cggccccaat taatatcgcc
     1321 gccgctttca taacgatcgc gtctccaaaa aatcaggggc ccattgtaat gcatcggaag
     1381 gaaacagcat gcgctcaacg cttgggttga aatgtttccg gcgggtccag ataacattgc
     1441 ctccagaatg cttcttccga aatttcaccc ttcgccaatt gctggatgct tgccagcgtt
     1501 gcgcctgtaa ggcctccgtc ggcggaatca aaaattacca caacgccatc caccgatgga
     1561 gcgcccggcg tcacgtcttc cacaaacttt tgcatcaacg agcctgcaaa atttagtttt
     1621 gcgcgcgcat cggtgtcctt tggatcctga ctggccggtt gcacagtgta gatcttgacc
     1681 gtttttccct tcagggcttc ccacccgcca gggaaaacac tgttgtcttc tcgctccagg
     1741 cggaaagccg ccggctttgc ggcactcttg gcttctgcag ttgcttccag gcgctcagcg
     1801 attacttcgg gcaattccag cgcgctcttg cgaatgtatc cacgcgacac tccggaaatt
     1861 tgtacgtgaa cccattcgcc gtcggcatcc agaaactcaa actcatcgtt caccgcggcc
     1921 ttaaacaaaa cgcgactctc gtcgcccgcg cgagccatca ttggcgttcc cgctctcttc
     1981 accgtcacga ga
//
LOCUS       DATCBB010000994         1051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_96201, whole genome shotgun sequence.
ACCESSION   DATCBB010000994 DATCBB010000000
VERSION     DATCBB010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96201"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VII37_10905"
     CDS             <1..894
                     /locus_tag="VII37_10905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY5070295.1"
                     /translation="EEKVLVNDLPGKASLRLRGNERITVLGAVQLPPLRAIPEEIPLD
                     IVFEDDDLAVINKPAGMMVHAGAGATEAARNRGTLVNALLHRFRKLSQLGGEMRPGIV
                     HRLDKATSGLLVVAKNDEAHRRLSAQFAKREVKKKYIALAHGWVKQNKGTINSAIGRD
                     RVHRTKMTTRHETGREAITHFTVQERIDSPVGKFTLLEVKIDTGRTHQIRVHLSSLGH
                     PVVGDALYGAPKVLRGKNQTIASLSRNFLHAAALQFQHPRTAEELSFLAPLPAELQAF
                     LVHYSEPIPVQRNAGKDGPSK"
BASE COUNT          250 a          291 c          299 g          211 t
ORIGIN      
        1 gaggaaaaag tgctggtgaa cgatttgccc gggaaggcct cgctgcgcct ccggggaaat
       61 gagcgcatca cggttcttgg cgcggtgcag cttcccccgc tgcgagcgat tccggaagaa
      121 attccgctgg acatcgtttt cgaagacgac gatttggcgg tgatcaataa acctgccgga
      181 atgatggtgc acgccggtgc cggtgctacc gaagccgccc gaaaccgggg caccctcgtg
      241 aacgccctgt tacaccgctt tagaaaactt tcgcaactgg gaggagagat gcgtcccgga
      301 atcgtgcatc ggctcgacaa ggccaccagc ggactgctcg tcgttgcgaa aaatgacgag
      361 gcccaccgcc gattgtcggc gcaattcgcg aagcgcgagg tcaaaaaaaa atacattgcg
      421 cttgcgcacg gatgggtgaa gcagaataaa ggaaccatca acagcgcgat tggccgcgat
      481 cgcgtgcatc gtaccaaaat gacaacccgg catgaaaccg gacgcgaagc catcactcat
      541 ttcaccgtcc aagaacggat tgattctccc gttggcaagt tcacactgct ggaggtgaaa
      601 atcgacaccg gacggactca ccaaatccgc gtgcatctgt cctctctcgg acatcccgtc
      661 gtaggcgacg ctctctacgg tgcccctaaa gttctgcggg ggaaaaatca gactattgct
      721 tctctttcga gaaacttttt gcatgcggcg gcgttgcagt ttcagcatcc gcggacggcg
      781 gaagagctat cattcctagc tccattgcct gcggaattgc aggcgtttct agtccattat
      841 tctgagccca ttccagtgca gagaaacgca ggcaaggatg gtccgagcaa gtgattgcat
      901 taagacaatc ccgcaatgcg ggcgcggcgg tacttacgat tctgtttata gctgtgctga
      961 gcgcgaaggc gggtccaccg tttcagacag acgacccgga accggtagac ttcaggcact
     1021 acgagtttta cgcatttgga aacgccagcg g
//
LOCUS       DATCBB010000995         1030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_290574, whole genome shotgun sequence.
ACCESSION   DATCBB010000995 DATCBB010000000
VERSION     DATCBB010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290574"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VII37_10910"
     CDS             <1..199
                     /locus_tag="VII37_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070296.1"
                     /translation="GGDTYQQWEFIWNPTGQVAMPAQTGANPNVNPTAPPNGTDPNNL
                     NGQPPPPTPPTPSVNVPSSTQ"
     gene            complement(425..>1030)
                     /locus_tag="VII37_10915"
     CDS             complement(425..>1030)
                     /locus_tag="VII37_10915"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5070297.1"
                     /translation="VLAPCARADYVVLQSGQRIHVTGYERVGDSLRLTLTGGTLEIPA
                     DALVRVDPEDTFVPVKVRLLDVPFANFISDSARVHGVAPELVASVIAVESNFNPNAVS
                     WRAARGLMQLMPETAARFGVTRVFDPQQNIEAGTKYLKELLTRYNGDLSLTLAAYNAG
                     PDRVEQYRSVPPFRETRDYVRRVTEKFRKTYRASNQTVELR"
BASE COUNT          252 a          293 c          286 g          199 t
ORIGIN      
        1 gggaggcgac acctaccagc agtgggaatt catctggaac cccacggggc aggttgccat
       61 gccggcacaa acgggcgcaa acccgaacgt gaatcccacg gcccctccga acggaaccga
      121 tccgaataac ctgaacgggc agccgccgcc gccgaccccg ccaacgccaa gcgtcaacgt
      181 gccgtcttcg acccagtaga gcaggcttca agcctgttgg gtttaacttt tccatacgcc
      241 aagaaaaatc aacaggctga acagattgcg gaaaaagtaa tttattttgt cattccgaac
      301 aaagtgagta atctctcctt ggtttaaacg caggaaaaga gagattcctg ggcgcgcagc
      361 gcgcctcgga atgacaaaaa taagggtttt tccgcaagct gttgaagcct gttctactta
      421 aaaactaccg caactcgacg gtttggtttg acgcgcgata agtcttgcgg aatttttccg
      481 tcacgcggcg aacgtagtcg cgcgtttcgc ggaagggagg aacggaacgg tattgctcga
      541 cgcggtcggg gccggcatta taggccgcca gcgtcaggga aagatcgccg ttataacgcg
      601 tcagcaattc cttcaggtat ttcgtgccgg cttcgatgtt ttgctgggga tcgaacactc
      661 tcgtcacgcc aaatcgcgcc gccgtttccg gcatcaattg catcaggccg cgagcggctc
      721 tccaggacac cgcattcgga ttgaagttgg attcgacggc aatcacgctg gccacgagtt
      781 ccggagccac tccatggact cgcgcggagt cggaaatgaa attcgcaaaa ggaacatcga
      841 gcaatctcac tttgaccgga acaaatgtgt cttccggatc gactcgcacg agcgcgtccg
      901 caggaatttc cagagtgccg ccggtcaacg tcaggcgaag ggagtcaccc acccgttcgt
      961 atccggtcac atgaatgcgc tggccggatt gcaggaccac ataatcggca cgcgcgcacg
     1021 gagcaagaac
//
LOCUS       DATCBB010000996         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_355271, whole genome shotgun sequence.
ACCESSION   DATCBB010000996 DATCBB010000000
VERSION     DATCBB010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355271"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VII37_10920"
     CDS             <1..1048
                     /locus_tag="VII37_10920"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY5070298.1"
                     /translation="QTRRGFIGAGQLEEYSAGVIYRHEQTLSGPKADRLELLRHTQMA
                     TGQLFFIYSDAQRRVDGVLNDAEKSAAPATEMTDEYGVVHRLWLISDAERVKAIQEAM
                     ADQKLVIADGHHRYETALNYRNERRKDAGKTIADAPYERAMMTFINTQGAGLTILPTH
                     RVVSKLREFNWTELRRHLEPWFTAESIEFGDESEKGKAREEFVRKLSAARRKRAIGAY
                     PAVAGEEKRAFYVLTLRQGVDLSRLLHNVSPLQRELDVVLLHDGILEPGLGITPQSVK
                     AEANLSYEREASAALDAVDSGAAQIAFLLNACDVEEVVKIATAGEVMPQKSTDFYPKL
                     MSGITMYSVDPERA"
     gene            complement(1178..1522)
                     /locus_tag="VII37_10925"
     CDS             complement(1178..1522)
                     /locus_tag="VII37_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5070299.1"
                     /translation="MALKFTNREVDGVSVVALDGRIVLGEESNALRERVKALVAEGKK
                     KIVLNMSNVTFIDSAGLGTLVAAHHSAKSQGAALKLCNLGAKFQEVLQITKLLTVFDV
                     YNSEPEAIASFK"
BASE COUNT          475 a          486 c          712 g          454 t
ORIGIN      
        1 tcagacgcgg cgcgggttta tcggggctgg gcagctggag gaatattccg cgggtgttat
       61 ttaccggcac gaacaaacgc tttcggggcc aaaggcggac cggctggaat tgttgcgaca
      121 cacgcagatg gctacaggac aattattttt tatttattcg gatgcgcagc ggcgggtgga
      181 tggggtgttg aacgacgcag agaagtcggc ggctccggcg acggagatga cggatgagta
      241 tggggtggtt caccgattgt ggttaatttc cgacgcggaa cgtgtgaaag cgattcagga
      301 agcgatggcg gaccaaaaac tggtgattgc ggacgggcat caccgctatg agacggcatt
      361 gaattatcga aacgagcgtc gcaaggacgc aggcaaaaca attgcggacg cgccttacga
      421 gcgggcgatg atgacgttta ttaacacgca aggggcgggg ttgacgattc ttccgacaca
      481 ccgggtagtt tcaaaattac gcgaatttaa ttggacggaa ttgcgcaggc acctggagcc
      541 gtggtttacg gcggaaagca ttgagtttgg cgatgagagc gagaagggta aggcacgaga
      601 agagttcgtg cgtaaacttt cagcggcgcg aaggaagcga gccattgggg catatccggc
      661 ggtggcgggc gaggagaagc gcgcgtttta tgtgctgacg ctacggcagg gggtggattt
      721 gagtcggctt ttgcataacg tttcaccgct gcaaagagag ttggacgtgg tgctattgca
      781 tgacggaata ctggagccgg gattggggat cacgccgcaa tcggtaaagg cggaagcgaa
      841 tctcagttat gagcgggagg cttcggcggc gcttgacgcg gtggatagcg gggcggcgca
      901 gattgcgttt ttgctgaatg cttgcgacgt ggaagaggtg gtgaaaatcg ccacggctgg
      961 agaagtcatg ccgcagaagt ccacagattt ttatccgaag ctgatgagcg gaattacgat
     1021 gtacagcgta gatccggaac gcgcgtgatt ggcgaacacg tgggcatacg acgcgacgca
     1081 agcccacaca acaacggcct gcggggtttt cgctgcggaa atttaaaccc cgtgccattg
     1141 tttagaggac ggtgaggtac gaacggttcg tgtttggtta cttgaagctg gctatggctt
     1201 cgggttcgct gttatagaca tcgaaaactg tcagcagctt ggtgatctgc aggacttcct
     1261 ggaatttggc gcccagatta caaagcttta gagcggcgcc ttgggacttg gcgctatgat
     1321 gggcggcgac cagggtgccg aggccggcgc tgtcaatgaa cgtgacattg gacatgttca
     1381 ggacgatttt ctttttgcct tcggcgacca aagccttcac ccgttcgcgc agtgcgttgc
     1441 tttcttcacc tagcacgatg cggccatcca gggctacgac ggagacgccg tccacttcgc
     1501 gattcgtaaa tttcaatgcc actttagttt cctcctcgtg gaaaacaggg ccccggacct
     1561 aaatgaaccg gcgccgcaaa gcttatcaat aatagtccgc gcaggacatg taataaccgc
     1621 ataccgcaca aatcaccttg caacggcggg actccagccg ctgagagcag acgggacaat
     1681 agtgcgaagc gtgttcgtaa ggcgcgaccg cggaagtaaa agcattcgag gagcgcatcg
     1741 aatgatccag ctgggaagcc tcgtaggtcg gcacctgctt cgtagtgggt tgcacggttc
     1801 gaaggttata gcacatcccc ggagaacggg gaagcgttgc gggtagagta tgtggttatc
     1861 tcaaatttag tttttaggat tccgtcagtt agttgcgacg acacgcaagg cattttcggt
     1921 cagcggcgag ggacgctcgc cgggctcacc tggtgggcgg gctatggttg cgtggtgccc
     1981 aagtaggtgg ccaggaacca ggtgttgccg aagggatcgg taacggttgc ggcccggtcg
     2041 ccgtatggcg cggtgtacgg tggagttatt cctttcgcac cggcagcaac ggctcgggcg
     2101 tagagggcgt cggtgtcggg cacatag
//
LOCUS       DATCBB010000997         1134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_355306, whole genome shotgun sequence.
ACCESSION   DATCBB010000997 DATCBB010000000
VERSION     DATCBB010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355306"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VII37_10930"
     CDS             <1..966
                     /locus_tag="VII37_10930"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY5070300.1"
                     /translation="GDGDYGRPTSLPWGMSFPNGVVPTAEHVHPTPLYEFFIWMAIAA
                     FLWHMGKKSLQKPKAKGEIFCNYLILTGIARFLIEIIRINPRSFFGLSNAQAASVASI
                     LAGAVLLWRIKSKFYALKKEHRIVEHIAAHGDILQPEYSRPTPECPYPERWNMYDSMS
                     AEVEVLDFLCQLVKTVKPELIVETGTFSGLSTLRLAEGLKANGVGRVITCEFDPKVFA
                     AAKQRFDSSGLGQWIDARNVSSLEMSVEGTIDMLFCDSDAPLREQEVRRFLPQMNPWG
                     LILMHDANSAMKIVRESALRLEAEGLITALLLPTPRGLVLAQKKN"
BASE COUNT          250 a          284 c          319 g          281 t
ORIGIN      
        1 ggcgatggcg attacgggcg gccgacgtca ctgccttggg gaatgagctt tccaaacggc
       61 gtggtgccca ctgccgaaca cgtgcatcca acgccgctgt atgaattttt tatctggatg
      121 gccattgctg catttctgtg gcacatgggg aagaaatcgc tgcagaaacc caaggccaaa
      181 ggcgagattt tctgcaatta tttgattctc accggtatcg ctcgttttct gatcgaaatt
      241 attcgcatca atccgcgttc gttcttcggc ctttcgaacg cgcaggcggc aagcgttgcg
      301 tccatcctgg ccggcgcggt gttgctttgg cgtattaaaa gcaaattcta cgcgctgaag
      361 aaagagcatc gcattgttga acacattgct gcgcacggcg acattctgca gccggaatat
      421 tccaggccca cacctgaatg cccttatccc gaacgctgga acatgtacga ttccatgagc
      481 gccgaagtgg aggttctgga ctttctatgc cagctggtaa aaaccgtaaa accggaattg
      541 atcgtggaga ccggcacttt ttctggcttg agcacgctgc ggcttgccga gggcttgaaa
      601 gccaatggag tgggccgggt cattacctgc gagtttgatc ccaaagtttt tgccgctgca
      661 aaacaaagat tcgactcttc cggtctgggg caatggattg acgcccggaa tgtttccagc
      721 ctggaaatga gcgtggaagg caccatcgac atgcttttct gtgacagcga tgctccgctg
      781 cgagagcagg aggtgcggcg atttctgccg cagatgaatc cgtggggatt gatcttgatg
      841 cacgatgcta actcggcgat gaaaatcgtg cgcgaatcgg cgcttaggct tgaagcggaa
      901 ggcttgatca ccgcactgtt gttgccgacg ccgcgtggat tggttttagc gcaaaagaaa
      961 aactaacttt ttggtgaagc agttgccggg gtaactttcg atttttacag catctggcgc
     1021 aaaacgtatt gcaggatgcc cccatggcgg taatacgcaa cttcttcagg cgtatcagcg
     1081 cgggcgatgg cggaaaaagt tttttccttc ccgcccgcca gtttcgcttt tacg
//
LOCUS       DATCBB010000998         1303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_161914, whole genome shotgun sequence.
ACCESSION   DATCBB010000998 DATCBB010000000
VERSION     DATCBB010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161914"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>1303
                     /locus_tag="VII37_10935"
     CDS             35..>1303
                     /locus_tag="VII37_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY5070301.1"
                     /translation="MSCVSQISEFAAGDTVLSVVERALQNAAVPQSERAGRIAETLGR
                     VGFTDREVLAATLSGGWKKRLAIAESLVQHPDVLLLDEPTNHLDLAGIAWLEGLLRNA
                     AFACVVVSHDRYFLENVASEVVELNRLYADGLLRVEGNYSTFLEAKELYLHAQRKEQA
                     ALANRVHTEIEWLRRGPKARTTKSKARIDKAHELIGDLADLNARTRSTSARIDFSATN
                     RQTKQLITLDGVTCDLGGRTLFKDLNFAVTAGMRVGLVGSNGSGKTTLLRLLRGEMAT
                     TSGRIQRAEPLRMVYFDQSRQLDPDLTLRRALAPDSDAVIYQGSVIHVAAWAARFLFD
                     PEDLNQRVGKLSGGERARVLIAQLMLQEADVLLLDEPTNDLDIPTLEILEESLLEFKG
                     ALLLVTHDRYLLDRVSTVVLGLDGRGGAERF"
BASE COUNT          284 a          312 c          425 g          282 t
ORIGIN      
        1 caccggagat gttgctgtgc gcaaaggaac gcggttgagc tgcgtctcgc agatttctga
       61 atttgccgca ggggacacag tgcttagcgt ggtggaacga gccctgcaga atgcagcggt
      121 gccgcaatcg gagcgagctg ggcgtattgc tgaaactttg ggtcgcgtag ggtttacgga
      181 tcgcgaggtg ctcgcggcaa cgctatctgg cggatggaaa aaacggctgg cgatcgctga
      241 atcgctggtg caacacccgg atgtgctgct gcttgacgag ccaacgaacc atctggacct
      301 tgccggaatt gcgtggctgg aaggcctgct gcgtaatgct gccttcgcat gcgtggtggt
      361 gagccacgac cgatattttc tagagaatgt ggccagcgag gtagtggaac tgaatcggtt
      421 gtacgctgac ggtttgctgc gagtggaagg aaactacagc acgtttctgg aggccaagga
      481 attatatttg catgcgcagc gcaaggagca agcggcgctg gcgaatcgcg tgcacacgga
      541 aatcgaatgg ctgcgtcgcg ggccgaaagc tcgtacgaca aaatctaaag cacgcatcga
      601 taaagcccac gaacttattg gcgacctcgc ggatttaaac gccagaacgc gaagcacgag
      661 tgcgcgcatc gatttctccg ccactaatcg ccagaccaaa cagcttatta ccctggacgg
      721 cgtgacttgc gatttgggcg gacgcacttt gttcaaggat ttgaacttcg cggtgactgc
      781 aggcatgcgt gtgggactgg taggatcgaa tggcagcggc aagaccacgc tgttgcgatt
      841 attgcgtggc gaaatggcga ccacgagtgg aagaattcaa cgcgccgagc cgctacgcat
      901 ggtgtacttt gaccagagtc gacagctcga tcccgattta acgctgcgtc gtgcgctggc
      961 gccggacagc gacgcggtga tttatcaagg gagcgtgatt catgttgctg cgtgggcagc
     1021 aagatttttg ttcgaccccg aggatctgaa tcaacgtgtt ggcaaattgt ctggtgggga
     1081 gcgtgctcgc gttttgatcg cgcagctgat gctgcaggaa gctgacgtac tgctgctgga
     1141 tgagccaacc aatgacctgg atattccgac gctggaaata ttggaagaaa gtttgctgga
     1201 atttaaaggc gcgttgctgc tggtaacaca cgaccgctac ttgctggatc gcgtgtcgac
     1261 ggttgtgctg gggctggatg gacgtggcgg cgcggagcgc ttc
//
LOCUS       DATCBB010000999         1803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_226914, whole genome shotgun sequence.
ACCESSION   DATCBB010000999 DATCBB010000000
VERSION     DATCBB010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226914"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VII37_10940"
     CDS             <1..1091
                     /locus_tag="VII37_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5070302.1"
                     /translation="VVAPAGGVNAPLVGIARLAAKSPAAETRRTSSERPEYFLLPVKS
                     ILNRCDSQRVPFEWTINPYRGCEFGCHYCYARYTHEYMELDGGEFEKKIYVKKDAAAL
                     LAWDVARKYSYQSQASGGEHPDHIAIGTATDPYQPAEKEYGVTRACLEELAKREGLSI
                     SVITKSNQIVRDIDVFKRIAERSSLSLNVTITTLRPRLARLLEPRAPRPDLRVAAVKQ
                     LSDAGLAVGVSASPLIPGITDREGDLEAVAAAVRAAGAQWFFSNALFLMPSSAKQFLP
                     FVRQKFPRLAKQYQEWYAKNGYAPEEYRRKVAERVAKIRKEHGFSTRPWEERTRTGGC
                     PQMSLSWNGGLGKRGLEQIQNCSLSKAS"
     gene            complement(1131..>1803)
                     /locus_tag="VII37_10945"
     CDS             complement(1131..>1803)
                     /locus_tag="VII37_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5070303.1"
                     /translation="VLADRASFAVDMRPKLLFNPDSRSPNFLLPGLTAILLLNVTTFL
                     TAFAIVREKERGTLEQLFVTPVRPFGLLLGKLLPYFFIGFTELCLILTCMRFIFQVPI
                     HGSVLLLAFLSLPYLFVSLSLGILISSKAGTQGEAMQLAFVTILPSIFFSGYIFPRET
                     MPIFFRVISYFVPASYFINITRGIILRGAGIQHLWIDGLALFAMGTFLLIVAARRFQN
                     KVIMA"
BASE COUNT          454 a          442 c          554 g          353 t
ORIGIN      
        1 ctgttgtggc gcctgccggt ggcgtaaatg cgccgttggt aggtatagcc cgcctcgcag
       61 caaaatctcc tgcggctgaa actcgccgaa cttcgtctga gcggcctgaa tactttctgt
      121 tgcctgttaa atctatcctg aaccgctgcg actcgcagcg tgtgccattc gagtggacca
      181 tcaatccata tcgcggctgc gaattcggct gtcattactg ctacgcgcgg tacacccatg
      241 aatacatgga gctggacggc ggcgaattcg agaaaaagat atatgtaaaa aaggacgcag
      301 cggcattgct ggcgtgggat gtggctcgca aatattccta tcagtcgcag gcttccggcg
      361 gtgagcaccc ggaccacatc gccatcggaa cggcgaccga tccttaccaa cctgcagaaa
      421 aggaatatgg tgtcacccgg gcgtgcctgg aggaattggc gaaacgcgag ggcctgagca
      481 tttccgtaat taccaagtcc aatcaaatcg tgcgcgacat tgacgtgttc aaacgcatcg
      541 cggaacgttc ttcactttct ctcaatgtaa ccattacaac gttgcgtcca agactggcgc
      601 ggcttttgga gccgcgagct ccgcgcccgg atttgcgcgt ggcggcggtg aagcagttga
      661 gcgatgcggg actagctgtg ggagtttcgg cgtcgccgct gattcccgga ataaccgacc
      721 gtgagggaga tttggaagct gtagctgcgg cggtgcgtgc ggcgggcgcg caatggtttt
      781 tctcgaacgc gctatttctg atgccctctt ccgcgaagca gttcttgcca tttgtgcggc
      841 agaagtttcc gcggctggcg aagcaatatc aggagtggta cgcgaaaaac ggatacgcgc
      901 cggaggaata ccgtcgcaaa gtcgcggagc gcgtggcgaa aattcgcaaa gagcacgggt
      961 tttccacgcg accgtgggag gaaagaacca gaacaggcgg ttgcccgcag atgtcgctgt
     1021 cttggaatgg cggactcggg aaacgcgggc tggagcagat tcagaattgt tctcttagca
     1081 aagcgagtta ggcggcacga gaaaccagcc gcaccaagct gaaatagttc ttaggccatg
     1141 atgactttgt tctggaagcg gcgtgccgcg acaatgagca aaaatgtgcc catggcgaaa
     1201 agggccagac cgtcaatcca tagatgctgg attcctgcac cacgaaggat gattccccgg
     1261 gtgatgttga tgaaatagct ggctggaacg aaatagctga tgacgcgaaa gaagatgggc
     1321 atggtctcgc gcggaaagat atagccggaa aagaaaatgc tgggcaggat ggtgacgaac
     1381 gcgagttgca tggcttcccc ctgcgtgccg gccttgctgg aaattaaaat ccccagtgaa
     1441 agcgatacaa acaggtaagg caaggagaga aaagcgagca gcagaacgct tccgtggata
     1501 ggcacctgaa aaataaaacg catgcaagta agaatcaggc acagctcagt aaacccgatg
     1561 aagaaatacg gaagcaattt tcccagtagc aatccgaacg gacggaccgg cgttacgaac
     1621 aattgctcaa gcgtgccgcg ttccttttcg cgaacgatag cgaaggcggt aagaaacgtg
     1681 gtgacgttta gaagcaggat ggcggtaagt ccgggcagca agaaatttgg ggagcgcgag
     1741 tccggattga atagcaattt cggccgcatg tccacggcga aggaagcgcg gtcggcgagg
     1801 acg
//
LOCUS       DATCBB010001000         1380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_322937, whole genome shotgun sequence.
ACCESSION   DATCBB010001000 DATCBB010000000
VERSION     DATCBB010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322937"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VII37_10950"
     CDS             <1..669
                     /locus_tag="VII37_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070304.1"
                     /translation="PAESDDITGGIGWEGLAKLQSFVNDGGLLVTLGSGSMLALEGGI
                     VRGVRRVSGGVPRSSAGGGSDSAAAAMESVTRTPGAHVRATFARPDHPIAYGYPVRTT
                     LFRQNFPLYDTPRSWLRMAYCTTCLDGPVDHSTVVLEWGDREGAPLLVSGQVWGPAGL
                     IGRPAILDMPAGKGHVVAFNFNPFHRDLNRGDHRLVWNAIINWQAILHQKTTPPSGPL
                     EETE"
     gene            complement(727..>1380)
                     /locus_tag="VII37_10955"
     CDS             complement(727..>1380)
                     /locus_tag="VII37_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5070305.1"
                     /translation="FRLAVYYLLAWPATYLLAAPRVRGRENLRGLDGPVLVISNHVTY
                     LDIAWILPALPARFRNRLATAMGGERLARMRRPAKEMNVFGRLLERLDYFLVLSLFNV
                     FPLPQQSGFLKSFSFAGDLADRGWNVLVFPEGQTTIDGNILPFRSGIGLLAKQLNIPI
                     VPMRLEGLYDLKQKNRSFARPGHVKVVIGQPVRFSPDQDANEITRDLERRVRGLGSP"
BASE COUNT          309 a          403 c          403 g          265 t
ORIGIN      
        1 cccgccgaat cagacgacat caccggaggc atcggctggg aaggcctcgc caaactgcaa
       61 tccttcgtga acgatggcgg cctgctggtt acgttaggca gtggttcgat gctcgcgctt
      121 gaaggaggga ttgtgcgcgg cgtgcgacgc gtttcgggtg gcgtcccgcg cagcagtgcg
      181 ggcggtggct cggactccgc tgcggcagcc atggaatcgg tgacgcgtac gccgggagca
      241 cacgtgcgcg cgacattcgc gcggcccgat caccccatcg catatggcta tccggttcgc
      301 accacgttgt tccgccaaaa tttcccgctt tacgatacgc cgcgaagttg gctgcgcatg
      361 gcttactgca ccacgtgtct cgacggtccc gtggatcaca gtacggtcgt gctcgaatgg
      421 ggtgatcgcg aaggagctcc gctcctggtg agtggccagg tgtggggccc agccggctta
      481 atcggtcgtc ccgcaatttt agatatgccc gccgggaaag gccacgtcgt agctttcaac
      541 ttcaatccgt ttcatcgcga tctaaatcgc ggcgaccacc gcctggtctg gaacgccatc
      601 ataaattggc aagccatcct gcatcaaaaa acaactccgc ccagcggccc actagaggaa
      661 accgaatagg gtatgacttg gcgcttatgt gttacgcgtt gcgccgcgct atctgagaaa
      721 aaatactcac ggactgccta gacctctcac gcgccgctca aggtcccgcg taatctcgtt
      781 tgcgtcctgg tcgggagaga agcgaactgg ctgcccaatc accactttca cgtgccctgg
      841 gcgcgcgaag ctgcggtttt tctgtttgag atcgtatagc ccttcaaggc gcatggggac
      901 gatcgggatg ttcagttgtt tcgccagcag cccgatgcca gatcgaaatg gaagaatgtt
      961 tccgtcgata gttgtctgac cttccggaaa aactaaaaca ttccaaccac gatccgccaa
     1021 atcgccggca aacgagaaac tcttcaggaa gccagactgt tgcggcaacg gaaatacatt
     1081 gaacagcgac aacaccagaa aatagtcaag gcgctcgaga agacgcccga acacattcat
     1141 ctcttttgcc gggcggcgca tgcgggccag ccgttcgcct cccatagcgg tggccaagcg
     1201 gttcctaaaa cgcgcgggta aggctggaag aatccaggca atatccagat aggtcacatg
     1261 attggagata accagaaccg gcccgtcaag tccgcggaga ttttcgcggc cccggacgcg
     1321 aggcgccgca agaagataag tcgcgggcca ggcgagcaga tagtagacag ccaagcgaaa
//
LOCUS       DATCBB010001001         1257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_387573, whole genome shotgun sequence.
ACCESSION   DATCBB010001001 DATCBB010000000
VERSION     DATCBB010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387573"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..>1257
                     /locus_tag="VII37_10960"
     CDS             43..>1257
                     /locus_tag="VII37_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070306.1"
                     /translation="MNFRTTNCALFKAAKIYTVRFSVPIAVALFVCVPGGVPQQQLDS
                     DAVINHLNAVIKLYGNASAELQIGGAPSDVIYQENEKSLAADVVKLAFQSARAEAAAS
                     LATEKNGAPSTSSGQSSAQTEARNKARIADIQSKLDSINKQIASAPKNKRDALNAQKE
                     ALEGELDLDNAVQEVIQKRAAFMESGMDTGAEGLEGRINHLAHSVPGVFAATNDQKIT
                     QAKPSPQSESAKPTPSGLVSQALALYAVIASVHQIDQLNDQTAAVKQIVNNLRQPLRD
                     SLVADIKKGRDFTDQAASGKIPPASPQQFQELTDHFKRISAVVLPLSQEIMVLDQATA
                     NLVEWRNAILRESKYAARALFVRVGMILLALGIVLALSEAWRRLTYKYIQDARRRRQF
                     LLLRRFVMGFLIG"
BASE COUNT          310 a          344 c          333 g          270 t
ORIGIN      
        1 aagttgccgt cgtggaataa atcctcggga gccaaacact gaatgaattt tcgcaccacg
       61 aattgcgcac ttttcaaagc agcaaaaatc tatacggttc gattttccgt gcccattgcc
      121 gtggccttgt ttgtttgtgt accaggtggc gtcccacagc agcagctgga cagcgacgcg
      181 gtaatcaatc atctgaatgc ggtcattaaa ctttatggca acgcctccgc tgaactgcaa
      241 atcggtggcg cccccagcga tgttatttat caggagaatg aaaaaagtct tgccgctgac
      301 gtggtgaagc tcgcatttca atcggccaga gcggaagctg ccgcgagcct cgccacagag
      361 aaaaacggcg cgcccagtac cagctctggc caaagctcag cgcagacgga ggctcgcaat
      421 aaggcgcgca tcgcagacat tcaatccaaa ctcgacagca tcaacaaaca gattgcttct
      481 gctcccaaaa acaagcgaga cgctttgaac gctcagaagg aagcgcttga aggtgaattg
      541 gatctagata acgccgttca agaggtgatt caaaaaagag ccgctttcat ggagagcggc
      601 atggataccg gggcggaagg actggagggg cgcatcaatc acttagccca ttcggtgccg
      661 ggcgtgttcg ccgctacgaa tgaccagaag atcacgcaag cgaagccgtc tccacaatcg
      721 gaatccgcga aaccgacgcc ctcgggcctg gtgtcgcaag ctcttgcact gtacgcagtc
      781 atcgccagcg ttcaccagat cgaccaattg aacgatcaaa ccgctgctgt gaagcagatc
      841 gtaaacaatc tgcggcaacc tctccgcgat tcactcgttg cagacatcaa aaaaggccgc
      901 gatttcactg atcaggcggc ttcaggcaaa attcctccgg cgtcgcctca acaatttcag
      961 gagctgaccg atcatttcaa gcgaatttcg gctgtggtgc tgccgctgag ccaggagatc
     1021 atggtgctcg atcaagccac cgccaatctc gtggagtggc gaaacgcaat tcttcgtgag
     1081 tcgaaatacg cggcgcgagc cttgttcgtg cgtgtgggca tgatccttct tgcgttgggc
     1141 atcgtactag ctttgtcgga ggcttggcgc cgtctcacct acaaatacat tcaggacgcc
     1201 cgacgccggc gccaatttct tttgctgcgt cggtttgtaa tgggatttct tatcggt
//
LOCUS       DATCBB010001002         1893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_227018, whole genome shotgun sequence.
ACCESSION   DATCBB010001002 DATCBB010000000
VERSION     DATCBB010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227018"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VII37_10965"
     CDS             complement(<1..353)
                     /locus_tag="VII37_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5070307.1"
                     /translation="MKVEQPKYKTSDDVDFVIIGSGAAGGILAKELSTNGFRVVVLEQ
                     GPYLTEADYTHNEIKVLGEDLLTNHPKLQPTTFRKTPQDKAKQQRALVYGRSVGGTSV
                     HFTANFWRFHEIDFI"
     gene            complement(350..1021)
                     /locus_tag="VII37_10970"
     CDS             complement(350..1021)
                     /locus_tag="VII37_10970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY5070308.1"
                     /translation="MPMINELSRRRFLSQAGIGVSAAWVSAHWPEMVSAAAHAHQALQ
                     SGAAYKFEFFTPAEATEIDALSARIIPTDDTPGAREAGVLYFIDRALVTFASGDQQKY
                     RDGLPELQSLVREKFPNVEKFSTATPEQQDELLRTMEASQTEPEKAPRRRSVKSAQTF
                     LEALRVQTIAGFLIDPEAGLGNRGGVGWKLIGREPEHSFQPPFGYYDKDYPGWKPAQK
                     VADKG"
     gene            1173..1790
                     /locus_tag="VII37_10975"
     CDS             1173..1790
                     /locus_tag="VII37_10975"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5070309.1"
                     /translation="MPTFTGGIGTGSKWLLDRIVGVVAATGVNPNVLTFLGLVVNFGA
                     AVSFAVGGFRTGALIILFAGFLDMLDGQVARRQNRVTAFGAFYDSTLDRYADMALYMG
                     LLVYYSVSGRTPYVVLAAVATAGSVMVSYARARAESLIPLCKVGFMERPERLVLLIIG
                     GLFNRMGAVLWVIAVVSTITVIHRIVYTWQETRAGRTLPGIKLTL"
BASE COUNT          372 a          546 c          504 g          471 t
ORIGIN      
        1 tcgataaaat caatctcgtg gaaacgccag aaatttgcgg tgaagtgcac gctggtgccg
       61 cccacgcttc gtccataaac cagcgcgcgc tgttgttttg ctttgtcttg cggggtcttg
      121 cgaaaagtgg tgggctgcag cttcggatga ttggtgagca gatcttcgcc caacacttta
      181 atttcgttat gcgtgtaatc cgcttcagta agatacgggc cttgttccag caccactacg
      241 cggaagccgt tggtcgataa ttccttggcc agaattccgc ccgcggctcc tgaaccgatg
      301 attacgaaat ccacgtcgtc ggacgtttta tattttggct gctcgacctt catcccttat
      361 ccgccacttt ctgcgcgggc ttccagcctg gataatcctt gtcgtagtag ccaaacggcg
      421 gctgaaaaga gtgttccggc tcgcggccaa tcagcttcca acctacgccg ccgcgatttc
      481 cgagaccggc ctcgggatcg atcaaaaatc cagcaatggt ttgaacgcgg agagcttcga
      541 gaaatgtttg cgctgatttc acacttcttc gccgcggagc cttttcaggc tcagtctggc
      601 ttgcctccat ggtgcgcaac agttcatcct gctgctccgg cgtcgcagtc gaaaatttct
      661 ccacgttggg gaacttttca cgtacgagac tctgcaattc gggcaagccg tcccggtact
      721 tctgctgatc cccgctggca aacgtcacca gcgcgcgatc gataaagtaa agtaccccag
      781 cctcgcgcgc gccgggcgta tcgtcggtgg gaatgatgcg cgcggagagt gcatcaattt
      841 ccgtcgcctc cgcgggagta aaaaattcaa atttgtaagc cgcgccggat tgcaaagcct
      901 ggtgcgcatg cgccgcagcg gaaaccatct ccggccagtg cgcggaaacc cacgcggcgc
      961 tgacaccaat acccgcttga gacagaaatc gacggcgact tagttcatta atcattggca
     1021 ttcagaactt ctaaggactg taggctacca agctttgcaa ggtcaacagc aaatcgctgg
     1081 tttgatcttg cggttttagt gatgcgatca ttcctgcggt cgcgacattt cgcgaatcgc
     1141 gcctggattt gagacactac tgaccgacgc atatgcccac atttacaggc gggattggca
     1201 ctggcagcaa atggttactg gaccgcatcg tcggcgtagt agctgccacc ggcgtcaatc
     1261 cgaatgttct tacttttctt ggcttggtcg ttaattttgg cgcggcggtc agttttgccg
     1321 tcggcggatt tcgaaccggc gcattgatta ttctttttgc aggttttctg gatatgctcg
     1381 acggccaggt ggcccgccgc cagaatcgcg tcactgcctt cggagcgttc tacgattcca
     1441 cgctggaccg ctacgcggat atggcgttgt acatgggctt gctcgtttat tattccgtga
     1501 gcggccgcac cccgtacgta gttctcgctg ccgtggctac cgcaggttcc gtaatggtca
     1561 gctacgcgcg cgcccgcgct gaatcgctga tccccctttg caaagttggc ttcatggagc
     1621 gccccgagcg cctggtgctt ttaattatcg gcgggctctt caatcgcatg ggcgcggtgc
     1681 tgtgggtcat cgccgtggtc tccaccatca ccgtaattca ccgcatcgtc tacacctggc
     1741 aggaaacccg cgccggccgg accctgccgg gaatcaagct cactctgtag tcagttcgtg
     1801 gactactaca tcaccgggta cgtatgaaaa agtcccataa ataattgcag atggatttcc
     1861 cctggttctc tcgagcatgt cctctacttc ctc
//
LOCUS       DATCBB010001003         1230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_387643, whole genome shotgun sequence.
ACCESSION   DATCBB010001003 DATCBB010000000
VERSION     DATCBB010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387643"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..>1230)
                     /locus_tag="VII37_10980"
     CDS             complement(129..>1230)
                     /locus_tag="VII37_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5070310.1"
                     /translation="GGRVKRHGSKDATVTMRDVAETSGFSPATVSIVLNNAPLARYIA
                     AATKKRIEETAKKLGYRPNAMARFLRSKRSHSVGVMIFDITDPYCTPVMRGIENALYQ
                     CSYVPIFTDAHNQRNRFERYLEMLLDRHIEALIVVANWLFVDIHLLADIGKRDIPAAT
                     IGWELPGDSVSSVMVDNEAGGRLALEHLVQLGHRKIACIRGPKMLIDSAPRWRGIQKS
                     AQAAGLEIDPALVVQLPDSLDPNFGFEGGQRFTDELLQKKKRFTALLAFDDLTALGAI
                     RALTKAGVKVPEQCSVIGFDDVPLSALAAPSLTTVRQPLEAMGNLAVNVIMEEVNANL
                     EKRDWNIVQHKMNPELVIRDSTRAASMAGSDD"
BASE COUNT          242 a          377 c          339 g          272 t
ORIGIN      
        1 aatgcgtcgc tggcttatct gccaaagtcg caatagccgc tggcgagcgg cagtccgttt
       61 tacccacacg cctttttcgg tgcgagtatt ttttttcatc ggcgtctgcg gacgatggcg
      121 cactcaactc aatcatcaga acccgccatg gaagcggctc gggtggaatc gcggatgacc
      181 agctccggat tcatcttgtg ctgcacaata ttccagtcgc gcttctccag attggcattc
      241 acttcttcca tgatgacgtt gacggccagg ttgcccatcg cttcgagcgg ttgacgcacg
      301 gtggtcagcg acggcgccgc cagcgctgac aacggcacat cgtcgaatcc gatgacggaa
      361 cattgctccg gcactttcac gcccgccttg gtcagcgcgc gaatggcgcc cagcgccgtc
      421 aggtcgtcga aggccagcag cgctgtgaag cgtttctttt tctgcaacag ctcgtcggtg
      481 aaacgctgcc cgccttcaaa gccaaaattc ggatcgagcg aatccggaag ctgcaccacc
      541 agcgccggat caatctccag gcccgcggcc tgcgcggatt tctggatgcc gcgccatcgc
      601 ggcgcgctgt cgatcaacat tttcggcccg cgaatgcagg cgattttgcg gtggcccagt
      661 tgcaccaggt gttccagcgc caggcgtccg cccgcttcgt tatccaccat cacggaactt
      721 accgaatcgc ccggcaattc ccatccgata gtagccgcgg gaatatcgcg cttgccgatg
      781 tccgcgagca ggtgaatgtc cacgaacagc caattggcca ccacaatcag cgcttcgatg
      841 tgccggtcga gcaacatttc cagataacgc tcgaagcgat ttcgctggtt gtgcgcatcc
      901 gtaaaaatgg gaacgtaaga acattgatag agggcattct cgatgccgcg cataaccggc
      961 gtgcaatacg gatcggtaat atcgaaaatc atcactccta cgctgtggct gcgtttgctg
     1021 cgcaaaaatc gcgccatggc gttggggcgg tatcctaact tcttggcggt ttcctcgatg
     1081 cgtttttttg tggccgcagc gatgtagcga gccagcggcg cattattcag cacgatcgaa
     1141 acggtagcag gcgagaatcc gctggtttcg gccacatccc gcatagtcac cgtggcgtct
     1201 ttgctaccgt gccgtttcac tcgccctccc
//
LOCUS       DATCBB010001004         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_31739, whole genome shotgun sequence.
ACCESSION   DATCBB010001004 DATCBB010000000
VERSION     DATCBB010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31739"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VII37_10985"
     CDS             complement(<1..685)
                     /locus_tag="VII37_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070311.1"
                     /translation="MSSVTKENEVLTAIPENAARPQPVALEVAVTVNGARAVAGSDKR
                     EPFSESTKTVLVFAHGAVVRLSSSVAPGQLLFLTNEKTKKEVVCQVVNSKNYSSVSGY
                     VELEFTEPVSGFWGMRFPAAPQGHSSVPPPASSPTARNLAIGSVAEPKIPVAATAIQP
                     ETKSTVPSSPRVSQTVPSSPAISSNQPSAPQSAALNSPPTSLNATPSYPSSAASTDAL
                     EIPKPIKTPT"
     gene            complement(853..1269)
                     /locus_tag="VII37_10990"
     CDS             complement(853..1269)
                     /locus_tag="VII37_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5070312.1"
                     /translation="MATFALIEYKDASPEVRAVYDDIMVTRKIDWINNFWKAIAHDPA
                     TLKRTWESLKQIMGPGALDPLTKELLYVAVSVTNNCNYCIASHTASARKKGMTDQMFH
                     ELQAVIGMANETNKLVTGYQVELDEQFKGPLPAPVR"
     gene            complement(1331..>2130)
                     /locus_tag="VII37_10995"
     CDS             complement(1331..>2130)
                     /locus_tag="VII37_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEY5070313.1"
                     /translation="FHAGRGLIGHNFGNLFLAALTHITGDFTEAIRESSKVLAIRGRI
                     FPSTLANVHLVATLTNGRRVRGETRITASRVPINKLELSPKNVRPLPLAIEAIRQADL
                     ILLGPGSLYTSVLPNLLIPEVASAIANSKSPLVYIANLMTQPGETAGYALADHLRAIQ
                     MHTSRKLVDYVVANRQPVSPKVARRYRAQGAEPVVINLPEIQKLGCRVVLDNLLDERD
                     VIRHNHKRLAKLLLEKFCCPTRKRSTHIISSRAISDRAASRRAGSKF"
BASE COUNT          459 a          509 c          670 g          492 t
ORIGIN      
        1 ccgttggcgt ctttataggt ttcggtattt caagagcgtc agtgcttgcg gcgctcgacg
       61 ggtaggatgg cgtggcgttc agcgaagtcg gcggcgaatt tagtgcagct gattgcggtg
      121 ccgacggctg attagaagat atagccgggc ttgacgggac agtttgggag acgcgcgggg
      181 aacttggaac ggtagattta gtttcaggct gaatggccgt ggccgctacc ggaatcttgg
      241 gctcagcgac cgaaccaatg gcgagattac gcgccgttgg cgatgatgca ggcgggggta
      301 ctgaactgtg accctgcggc gctgccggga agcgcattcc ccagaatccc gagaccggct
      361 cggtgaattc caactccacg tagccgctca cgctgctgta attttttgaa tttaccacct
      421 ggcatacaac ttcttttttt gttttctcgt tggtcaggaa tagcaattgg ccaggagcta
      481 ccgaagagga caaacgcacg actgcgccgt gcgcaaatac taaaacggtc ttagtggatt
      541 cagaaaaagg ttcgcgcttg tcgctcccgg caacggctcg ggcgccattc acggtgacgg
      601 ctacttccag cgccacgggt tgcgggcggg cggcattctc agggatggcg gttaatactt
      661 cgttttcctt cgtgacagaa gacatgagca ctctcgcgag aacaggttgc tccggcacac
      721 acaaacagag tgttcgcgcg gaatcacctt atggtgtaag gcacgcgggg tgctagctat
      781 agtactgagg tactatgtgg aaaaaaattt gtacagatag gcaccccggt cacgggccga
      841 aaagcacctg tactaccgga caggtgccgg cagggggcct ttaaattgct cgtcgagttc
      901 gacctggtag ccggtgacca acttgttggt ttcattagcc atgccgatca ccgcttgcaa
      961 ctcgtggaac atctggtcgg tcatgccttt tttgcgtgcc gaggcggtgt gcgaggcgat
     1021 gcagtaatta cagttgttcg tgacgctgac ggccacgtag agcagttctt tcgtcaaggg
     1081 gtccagagcg cccggcccca tgatttgctt aagcgattcc caggtccgtt ttaaggtggc
     1141 ggggtcgtgg gcgatggctt tccagaaatt attgatccag tcgatctttc tggtaaccat
     1201 gatgtcgtca tagacggcgc gaacttccgg gctggcatct ttgtattcaa tcagtgcgaa
     1261 ggtggccata atttctccag agaaaattca acgaatgagt gagtaaacgt tagcaggaac
     1321 gctaggaaat ttaaaatttg gaaccggcgc gcctggaagc ggcacggtcg gaaatggcgc
     1381 ggcttgaaat gatgtgcgtc gagcgtttgc gagtgggaca gcaaaatttt tcgagcagca
     1441 actttgctag gcgtttgtga ttgtggcgaa tgacgtcccg ctcatcgagc aaattatcca
     1501 gcacaacgcg acaacccaat ttttgaatct ccggcaaatt gatgactacg ggctcggccc
     1561 cttgcgcgcg gtagcgccgc gctacttttg gcgagacagg ctggcgattg gcaaccacgt
     1621 agtcaaccaa cttgcgagag gtgtgcattt gaatggcgcg gagatgatcg gctagcgcgt
     1681 aaccggcggt ttcgccgggc tgcgtcatca gatttgcgat gtaaacgagc ggggacttgg
     1741 agtttgcgat ggcggaagcc acttctggaa ttaagagatt aggcaagacg ctggtgtaca
     1801 gcgagccggg gccaagcaga attagatccg cttggcgaat ggcttcaata gcgagaggaa
     1861 gcgggcgcac gtttttcggc gagagctcta atttgtttat gggcacgcga ctggcggtta
     1921 tgcgcgtttc gccgcgcacg cggcggccat tcgtgagcgt ggccacgaga tgcacattcg
     1981 ccagcgtgga tggaaagatt cggccgcgga tggcgaggac tttgctgctt tcgcggattg
     2041 cttcggtgaa gtctccggtg atgtgggtga gcgcggcgag gaaaagattt ccaaaattgt
     2101 ggccgatgag tccgcgtccg gcatgaaaac
//
LOCUS       DATCBB010001005         1647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_64185, whole genome shotgun sequence.
ACCESSION   DATCBB010001005 DATCBB010000000
VERSION     DATCBB010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64185"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VII37_11000"
     CDS             <1..583
                     /locus_tag="VII37_11000"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY5070314.1"
                     /translation="CEIVDAIPFDQDHAHTAYDRVYANRFWRVLASTQQVMAHFRTGF
                     LGKVSPVHFFWGSFDLAVTRFSGRRAPRHPGGVPHLSDAVAREAYSHEVSSAGFWPGG
                     GGPVEYAAFYSYAYPAPEGFAEALVKPAEAFFSKELSEFILPYDAMRTARKPDGALMD
                     FLQSTYKAAADLAKWDRKMLECDLGEAGKVRMV"
BASE COUNT          341 a          480 c          470 g          356 t
ORIGIN      
        1 gtgcgagatc gttgacgcca ttccgttcga ccaggaccac gcgcacaccg cctacgaccg
       61 cgtgtacgcc aaccggttct ggcgtgtgct ggcctcgaca cagcaggtga tggcacattt
      121 ccgcactggc ttcctcggca aggtgagccc tgtgcatttc ttctggggca gcttcgacct
      181 agcggtgacg cggttctcgg gtcgacgcgc accacgccac cccggcggcg tgccgcatct
      241 ttccgacgcg gtggcgcgag aagcctattc gcacgaagtg tcgagcgcgg gcttctggcc
      301 gggcggcggc gggcccgtgg aatacgcggc gttttattct tatgcctatc cggcgccgga
      361 gggatttgcc gaggcgctgg taaagccagc ggaggcgttc ttcagcaaag aactgagcga
      421 gtttattttg ccgtatgacg ccatgcgcac ggcgcgcaag ccggacggcg cgttgatgga
      481 ttttctgcag agcacttata aggcggcggc ggatctggcg aaatgggacc gcaagatgct
      541 ggagtgcgac ttgggcgaag cggggaaggt gaggatggtg taacgatccg cctcatccta
      601 aggagcccgc agacaagagc gtgtggatgt ccgcttcggg tcttggccgt gtgaaaactc
      661 caacgcttgc cgcgcgcata ggataatttt gtaaaaattg cgcgtcatga gaactgataa
      721 tgctgtgggc gtatggccgg atgacgcgct gaagaatttt attttctaca ttttttcgat
      781 gtatgagttt tcacacagcc taggtcaatc gctgccgacc cattcagccc aaatgccgac
      841 ctttgtccgt tgttagtcca atagcgggtc acgctcgcca aggagcgctc ctaaactttc
      901 gcgccaccca cccggcggct acaactgaga aataagtaat taagcccatg agacctgcta
      961 cgagggtcgc caccccgagg tagcttgcga gcgttgcagt ataattaaag ccacccttct
     1021 gtgttgccgc tatctcctct gcggagagcc ccgacaacga taagccacac acaccttttg
     1081 ctacgctttg ttccggcgcg gagtcatttc ccgcgtgtag atcgttgtcc gacaaacact
     1141 gcaacgccag aaatcgagtt tgttggtcac tcacggtcct tatcgacatc gtgacgccaa
     1201 ctgccaaggc gcacaatccg attcccaccg cgagcgcaat accaacgcgg tggaggccgc
     1261 gcctcatttg cggcgaggtg gattggtcag tcatatttga ctagctccga gcaactctca
     1321 tgccccgcaa cgcgggccaa acgcaatgtc cgctattact ccaatagcga ccaaaatgtt
     1381 gcagtgccgc gaatgtccgc tttgtgccaa tagcggtcat ttccactgca acgccatgac
     1441 acaatagaag ccgcagattc aaaattggat gggtcaaacg gcgccggtgt gagttccacc
     1501 gtgcgcacta cagtggttga tcaccacgta tatcgcagct ttccggtgcc ggtgtaggtc
     1561 tgcgagccat tcgcgaactc gccgtcgaac ttcgccatta cggcccagcc acttctcagc
     1621 ttaatctcgg cgccagcggt gacgagc
//
LOCUS       DATCBB010001006         1573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_128861, whole genome shotgun sequence.
ACCESSION   DATCBB010001006 DATCBB010000000
VERSION     DATCBB010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128861"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(791..1282)
                     /gene="rpsT"
                     /locus_tag="VII37_11005"
     CDS             complement(791..1282)
                     /gene="rpsT"
                     /locus_tag="VII37_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY5070315.1"
                     /translation="MTLHGVASIRTPMAAGAIPPPLFFERRRHASVFACREWHCQGHP
                     KTRNRRSRIWTAARLKPDHLAIGHRRRIFENMANTSSAKKATRKIARRTIINKSRRTQ
                     MRGAVRTVEEAIKSGDRDAALRAMARAEPELMQAAQRNIIHRNNASRKVSRLAHQIAK
                     LAK"
BASE COUNT          309 a          462 c          440 g          362 t
ORIGIN      
        1 gcccactttg cttcgccttc ggcacccttt tcggctggct ggtgcgtatc tctcttgttc
       61 gaagcgaacg agacacgaat gtcccgtttg agaaatttcg acacagaacc acactcctca
      121 tattgctatg aggtacaacc caacaatcgc ttggaaagcg tcgccaacgc gcacaccgcc
      181 gccgatttgc acgtccgccc cgtttcttaa accgcgctgg tctgaagaac cgtgggcgcc
      241 gcgaacgtcc taagagatac atcatgcccc atttcccgca acggaaatga ttcagcgcga
      301 gcgccgcgat aactacttcg cgcttgcgaa atcaggcagc ggactttgtc gagcacggca
      361 aggttttgaa tctgtgcgga gattcgaaac acgcaggcgc tgtgacattt ctgacaactg
      421 ctcaaaataa attcataaat cgtttacgtt tgaatgtggc ggagaggatt tttcgaacaa
      481 ctcttcgcca ctatgccgat aagtgactac gcaagcgcgt ttttttttgc tcgtccgaag
      541 agggtaaccc acgctgctcg tgcgcgtgct cgcgccgttg cggttgctat ttcaaaagtg
      601 agcccgggta tgactgacgt caatttcgtg acgcgctgcc gcaaagccta agcccggctt
      661 cctacataca gccagtaaaa acacgggcgc gcaggaattt ttgcgagccg caatttacgg
      721 agcgcggtat ttcgcaaaaa taaaaagccc ggcaaggccg ggcttttgac gaatttcatt
      781 ttcgttcagt tcatttcgcc agcttggcga tctgatgcgc gagccgcgaa acctttcggc
      841 tcgcattgtt gcggtgaatg atgttgcgct gcgccgcctg catcagttcc ggctccgcgc
      901 gcgccatcgc cctcagcgcg gcatcgcgat cgccgctctt gatcgcctct tcgacggtgc
      961 gaaccgcgcc gcgcatctgg gtgcggcgcg acttgttgat gatggtgcgg cgggcaatct
     1021 tccgcgtcgc ctttttggcg gaactcgtat tggccatgtt ctcaaatatc cttcggcggt
     1081 gaccgatcgc caggtggtcg ggcttcaagc gcgcggccgt ccaaatgcgt gatcgacgat
     1141 ttcgtgtttt cgggtgtccc tgacaatgcc attctctgca agcaaatacg cttgcatgac
     1201 ggcgccgctc aaagaacagc ggcggcggga ttgcgcccgc cgccattggc gtccttatag
     1261 aggccacgcc atgcagcgtc aacgccttcc gggctccccc ggccccccgg gcacgggccg
     1321 gaaaggcgga taaggtgctg atgaggttga atttcccgaa ccgccccggt aagggctgac
     1381 agcgctttcg ggcgccagag atgtaaggtg acgcatgatc cgcggttttt tccgacttgt
     1441 gggactattg ctgctggccg gcggattcgt cttcatggtc tatgatgggg cacgctgggt
     1501 cgccgatcag accctgcgct tcacccgctt tggccagttc tggaacgata ttcaccagtc
     1561 cagccagcag gca
//
LOCUS       DATCBB010001007         1821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_323135, whole genome shotgun sequence.
ACCESSION   DATCBB010001007 DATCBB010000000
VERSION     DATCBB010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323135"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VII37_11010"
     CDS             <1..283
                     /locus_tag="VII37_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070316.1"
                     /translation="INLADNPRLDGMGFAAFGQVTDGMNVVDTLYADYGEGAPGGSGP
                     NQDEIQKQGKPYLDKNFPQLDYIKTTVLTPAPGPATHKTLPAKKTVPNP"
     gene            651..>1821
                     /locus_tag="VII37_11015"
     CDS             651..>1821
                     /locus_tag="VII37_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070317.1"
                     /translation="MAEVIPDRSTEAKPNEAKPVETKAQRVERLKRSKNAWDHFDEIQ
                     QFARTGFDSIPPEWLGTYFRSWGVYTQGDGVGVIGGKNGEGKAMPFFMVRIRIPNGLM
                     RSEQVRTIASLAEKYGRGVADITVRQNIQLHWVTIESLPDVLQSLWNVGLTTTGACGD
                     VARNITGCPLGGLDGDEIIDASPLALQIDRELGGNSEFYNLPRKFKISITGCRHWCSY
                     PEINDIGLTATTRRRAGKSEVGFTLRVGGGLSTNPHLAVALDAFIKPDQVIKVVRGVA
                     EIFRASDVLRVSREKARLKFLFLEHGWTADIFLAELQQRIGFSLDPGEREEIPADIHR
                     DHVGVHEQKQPGLVYVGASVLRGRITPQQLNLAADLADRYADGHVRNTVMQNLLIV"
BASE COUNT          444 a          540 c          456 g          381 t
ORIGIN      
        1 cattaatctg gccgacaacc cgcgcctcga cggcatgggt tttgccgcat ttggccaagt
       61 caccgatgga atgaacgtcg tcgacacttt gtacgccgac tacggcgaag gcgcgcccgg
      121 cggcagcggc cccaaccagg acgaaattca aaaacagggc aagccctacc tggacaaaaa
      181 tttcccccag ctcgattaca tcaaaaccac ggttttaacc ccggctcccg gcccagcaac
      241 gcataagact ttgcccgcga agaagacggt acccaatcca tagcgtattt cgccatattt
      301 gccggatata caggccgtag ccgaatctgc catgaatcag caatttgaat gcttctaatc
      361 gaatatttaa caatgtgttg atttgacacc cctttcgaaa tcatcgtaat ctattcctcg
      421 atgagtactt gttgccagat gttctgttgt gcgtgcccat gcaacgctgc gtgttgcacc
      481 gtccacagct ctggccaaac aagtttccga acttagcgca taaatactaa aaagttccga
      541 agcccacgtt gccagagcac tggtgaacgt gggctttttg tttttaggcg cgaagttaca
      601 aaaaacgaca aaaagcgcgc cacagaagca gtttcacagg ggaatcgttc atggctgaag
      661 ttattccaga tagatcgacc gaagctaagc ccaatgaggc gaaaccggtt gaaaccaaag
      721 ctcaacgcgt cgagcgcctc aagcgttcca aaaacgcctg ggaccatttc gatgagatcc
      781 agcagttcgc ccgcaccgga ttcgattcca tcccgccgga atggctgggc acctacttcc
      841 gctcctgggg cgtttacact caaggcgacg gagtcggcgt aattggcggc aaaaatggcg
      901 aaggcaaggc catgccgttt ttcatggtgc gcatccgcat tcccaacggc ttgatgcgca
      961 gcgagcaagt ccgcactatc gcgagtcttg cagaaaaata tggccgcggc gtcgcggaca
     1021 tcaccgtgcg ccaaaacatt caattgcact gggtcaccat agaatcctta cccgacgtcc
     1081 tacagagcct gtggaacgtt ggcctcacca ccacaggcgc atgcggagac gtagcgcgca
     1141 acatcactgg gtgcccgcta gggggcctcg atggcgacga aataattgat gcctccccgc
     1201 tcgcgctgca aatcgaccgc gaactcggcg gcaattccga gttttacaat ctcccgcgca
     1261 aatttaagat ttccatcacc ggatgccgcc actggtgctc ctatcccgaa atcaatgaca
     1321 tcggtctcac cgccacgact cgacgtcgcg caggaaagtc cgaagtaggt tttaccctgc
     1381 gcgtcggagg cggtctttcc accaatcctc acctcgcggt agcgctcgac gctttcatta
     1441 agccggacca ggtaatcaag gtagttcgtg gagtggcgga aatcttccgc gccagcgatg
     1501 ttttgcgagt gagcagggaa aaggcccgcc taaaatttct cttccttgaa catggctgga
     1561 ccgccgacat cttcctggcg gaattgcagc agcgcatcgg tttttctctt gatcccggcg
     1621 aaagggaaga gatcccagca gacatccacc gcgatcacgt cggcgtacat gagcaaaagc
     1681 agcccggtct cgtctacgta ggcgcgtcgg tattgcgtgg ccgcatcact cctcaacaat
     1741 taaatcttgc cgctgacctc gccgatcgct acgccgacgg tcacgttcgc aacaccgtga
     1801 tgcaaaacct gctcatcgta a
//
LOCUS       DATCBB010001008         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_323156, whole genome shotgun sequence.
ACCESSION   DATCBB010001008 DATCBB010000000
VERSION     DATCBB010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323156"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1179)
                     /gene="galT"
                     /locus_tag="VII37_11020"
     CDS             complement(154..1179)
                     /gene="galT"
                     /locus_tag="VII37_11020"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY5070318.1"
                     /translation="MPELRKDPITGRWVIISTERGKRPNEFLRESVVASNVKNCPFCP
                     GHESKTPTEILVYGRNGGSKNTPGWNIRVVPNKFPALGIEGDLDREGEGLFDKMNGVG
                     AHEVIVETPDHGSTLASLPERAVEDVLWAYRDRMLDLKNDKRFRYILIFKNHGEAAGA
                     SLEHPHSQLIALPIVPRRVREEVDNCWHYYDEKERCIFCDIIRQERDTGERVIGENEH
                     FITLAPYAPRFPFEMWLLPKTHGSSYENNQSSIYGSLARMLKDTLMRLDSVLDRPAYN
                     FMIHTSPIGEEINDHYHWHIEIIPKLTKVAGFEWGTGFYINPTPPEESARFLRETKID
                     SPLVNKK"
     gene            complement(1350..1871)
                     /locus_tag="VII37_11025"
     CDS             complement(1350..1871)
                     /locus_tag="VII37_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY5070319.1"
                     /translation="MPIFSRLIFTVVRWSARRGLRDSAAPAPQAQKQESQRDEQFERK
                     EKARRRGLLGETYGYWYLRRQGYVFVAKNYMPQGAKGELDLVGYDGETIAFVEIRTRT
                     VRDDISGLPELSVSYAKQSMLVRTAQRFLADRHLHDCPTRFDVLAIDNIPGRPPEIRL
                     HKDAFSPQLRRFG"
     gene            complement(1988..>2206)
                     /locus_tag="VII37_11030"
     CDS             complement(1988..>2206)
                     /locus_tag="VII37_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5070320.1"
                     /translation="IGIEIVHDQKTKEKGADLRNHIIDLAFRRGLCILGAGENTLRLS
                     PPLLIDREQADAALAILEQCIQEVEIKA"
BASE COUNT          500 a          595 c          591 g          520 t
ORIGIN      
        1 ctcgtgcgac cactgggcat cgctgcgagc agctgaaaat tgcccgcgct tacaattgaa
       61 gcccaaatga gaattatggt cgttaagttg tgaggtaccg aggaagcacc ggacgcgccg
      121 tctctcagtc aaccagcgct attccctgct cagctatttt ttgtttacca ggggcgagtc
      181 gatctttgtt tcacgtagga aacgcgccga ttcttcgggg ggcgtggggt tgatgtaaaa
      241 acccgtaccc cattcaaagc ccgcgacctt ggtgagcttg ggaataatct caatgtgcca
      301 gtgataatgg tcgttaattt cttccccgat cggcgaagta tgaatcatga agttgtacgc
      361 cggccgatcc agcacggaat ccagccgcat caacgtatct ttcagcatgc gcgccaggct
      421 gccgtaaatg gagctttgat tattttcata cgaagagccg tgggtcttcg gcagcagcca
      481 catttcaaaa ggaaaacgtg gagcatacgg ggccaaagta atgaaatgct cattttcacc
      541 gatgacccgc tcgccggtat cgcgctcctg gcgaataatg tcgcaaaaaa tgcagcgctc
      601 tttctcgtcg taatagtgcc agcaattgtc gacctcttcc cgcacgcgac gcggaacgat
      661 gggcagcgcg atcagttgtg agtggggatg ttccagcgaa gcgccggctg cttctccatg
      721 atttttgaag atgaggatat agcgaaagcg cttatcgttt tttagatcca gcatgcggtc
      781 acgataagcc cacaacacgt cttccacggc gcgctccgga agacttgcca gcgtggaacc
      841 gtggtcagga gtttcgacga tcacttcgtg cgcgccgacc ccgttcattt tatcgaacag
      901 cccttcccct tcgcgatcca gatcaccttc gatgcccagc gccggaaatt tgttagggac
      961 tactcgtatg ttccatcctg gcgtgttttt gctgccgcca ttgcgtccgt agaccaggat
     1021 ctccgtgggg gttttggatt cgtgcccggg acaaaacgga caattcttaa cgttggaggc
     1081 tacaacgctt tcgcgcagga attcgttggg gcgctttccg cgctccgtgg aaataataac
     1141 ccagcgccct gtgattggat ctttccgcaa ctcaggcaaa gctacctcct cgaccactta
     1201 ctactaaaat cggccagaca cacgaaagaa agtgcgccaa gcctacgcaa ttattaaatt
     1261 gtaccccgat tgcgccaggg cgcagattcg tgacgggcta tcctgcgctg cggatgttat
     1321 agccatttag agcaacatgg gtgagaaaac tatccaaagc ggcgcaactg cgggctgaag
     1381 gcgtctttgt gcagcctgat ttccggcgga cgaccgggaa tattgtcgat agccagtacg
     1441 tcaaagcgcg tggggcaatc gtgtaagtgg cggtccgcta agaagcgctg cgcggtacga
     1501 accagcatac tttgtttggc gtagctgacg ctcagctctg gcagaccaga gatatcgtcg
     1561 cggacggtgc gtgtgcggat ttctacgaag gcgatcgttt cgccatcgta tcccaccaaa
     1621 tccagttcgc ccttagcacc ttgcggcata taatttttgg caacaaagac atagccctgc
     1681 ctgcgcaggt accagtagcc ataagtttct cccagcaagc ctctgcgacg cgccttttcc
     1741 ttgcgttcga attgctcatc gcgctgggat tcttgctttt gtgcttgtgg cgcgggcgca
     1801 gcggagtcac ggaggccacg gcgcgcgctc caacgtacaa cggtaaatat taaacgcgag
     1861 aatattggca tgatttgcct gaggtagacg caaaccgtac acgcagtata taaagaaaat
     1921 cccgtggcgc ccaaacttct gtggacggcc acgggatcgc tgaaaactta agattcttga
     1981 gcccgcgtta tgccttgatt tccacttcct gaatgcactg ctcaagaatt gccagcgcgg
     2041 catcggcttg ttcgcgatcg atcaaaagcg gcggtgaaag ccgaagagta ttctcgcctg
     2101 cgccaagaat gcacaagccg cgacggaagg ccagatcgat gatgtgattg cgcagatcgg
     2161 cgcccttttc cttggttttc tgatcgtgca cgatctcaat tccgat
//
LOCUS       DATCBB010001009         1200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_193216, whole genome shotgun sequence.
ACCESSION   DATCBB010001009 DATCBB010000000
VERSION     DATCBB010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193216"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VII37_11035"
     CDS             complement(<1..1041)
                     /locus_tag="VII37_11035"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY5070321.1"
                     /translation="MTGNSISDDVTYRFRDLLALEPLSADELTFLLDQSKLFQEIQRH
                     PLKKLATLRGKTVTLAFFENSTRTRISFSTAASRLGADTMNLQAEASSLKKGESLLDT
                     VYTLNAMKPDCLVMRHAASGAADFVARNLDIPVINAGDGTHEHPSQGLLDALTIRDRR
                     GSIENLKVTILGDIQHSRVARSNIHLLGKFGGQFTLCGPAMWVPKELEKIGPPGCVIR
                     RVSRIEEGLEDADVILVLRVQTERMYEPTLAAEEYILLYQLNAERLRIANPNALILHP
                     GPMNRGVEISPEVADGPQSCVLEQVTNGIAVRMALLFLLMSGATEGGENPVADEALSG
                     SAASPEKKESTHA"
BASE COUNT          248 a          325 c          341 g          286 t
ORIGIN      
        1 agcatgcgtg gattcctttt tttctggaga ggcggccgag ccactcaatg cttcatccgc
       61 gacggggttt tctccgcctt cggtcgcgcc ggacatcagc aaaaacaata gcgccatgcg
      121 cacggcgatg ccgttggtca cttgttcgag gacgcacgac tgcggcccat cggctacctc
      181 cggcgaaatt tccaccccgc gattcatggg acctggatgg aggatgagcg cattgggatt
      241 ggctattcgc aagcgctcgg cattcagttg gtagagcaga atatattctt cggcggcaag
      301 tgtgggttcg tacatgcgtt cggtttgtac tcggaggacc agaatcacgt cggcatcttc
      361 aaggccttct tcgattcgcg agacgcgacg aatcacgcag ccaggagggc cgattttttc
      421 gagctccttc gggacccaca ttgccgggcc gcacagagtg aactggccgc cgaattttcc
      481 cagcaaatga atattcgaac gggccacgcg actgtgttga atatcgccga gaatggtgac
      541 tttcaaattt tcgatcgagc ctcgccggtc gcgaatggtc agcgcgtcca gcaacccttg
      601 cgaggggtgt tcgtgggtgc cgtcgccggc gttgatcacc ggtatatcaa gattgcgggc
      661 cacaaaatct gccgcgccgg atgccgcgtg acgcatcacc agacaatccg gtttcatggc
      721 attcagcgtg tagacggtat ccaggagcga ttcgcctttt ttcaagctgg acgcttccgc
      781 ttgcaggttc atggtgtccg cgcccaggcg cgaggccgca gtgctaaaac tgatgcgcgt
      841 gcgcgtggaa ttttcgaaga acgccagagt gacggtttta ccgcgcaatg tggctagctt
      901 tttgagggga tgccgctgaa tttcctggaa cagcttgctt tggtccagca gaaaagttag
      961 ttcgtccgcg ctgagcggtt caagagccag gagatcgcga aagcggtagg tcacatcgtc
     1021 acttatggaa ttgccggtca atcctcttat tgtcgaacag cggcgaagta ctgattaatt
     1081 ctgatcgatt ccggttaatt caccatggtc atgaaaatat tgctacggtt ccacgcgatc
     1141 gaccaacact acacgctctt cgctgtcgat ctcgcgcaac cgtacttcca cgatttctgt
//
LOCUS       DATCBB010001010         3341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_355659, whole genome shotgun sequence.
ACCESSION   DATCBB010001010 DATCBB010000000
VERSION     DATCBB010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355659"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VII37_11040"
     CDS             complement(<1..401)
                     /locus_tag="VII37_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070322.1"
                     /translation="MTKTPQHNVSNAERALLVGVGWKRAPRFPGMPAGEQGRVSLEEL
                     VELAKSAGAEIVGTVFQVRDSADSATLVGRGKLDEIRAEANAHQAPLIIFDSNLSPVQ
                     QRNIEELTERRVIDRTQLILDIFARHARSREG"
     gene            complement(499..1476)
                     /gene="miaA"
                     /locus_tag="VII37_11045"
     CDS             complement(499..1476)
                     /gene="miaA"
                     /locus_tag="VII37_11045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY5070323.1"
                     /translation="MNTIELESLPLIVVVGPTASGKSALAMALANVLQTQAEIIACDS
                     TQVYRGVNIGTAKPSVEERQGVVHHLIDVLDASEAATAGGYRDLALGVLADMRKRGRL
                     PILTVGTGLYVRALLEGLADLPLRSEELRERLRASSRKGGAMHLHGVLRRMDAEAAAK
                     IAPTDEQKLIRAIEVCILTRKPISEVHRAGRSPLQGWRTLKVGLMPSREMLCERIDAR
                     TEAMIESGWLDEVRGLLTAGITENAKIFDFIGYREMLGVLRGDLTMEKARAAIQLATR
                     QYAKRQMTWFRKDKSIRWFAGPGDDGKVQSDICEYVSKEIARGAPQIPR"
     gene            complement(1579..2961)
                     /locus_tag="VII37_11050"
     CDS             complement(1579..2961)
                     /locus_tag="VII37_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEY5070324.1"
                     /translation="MIERTLVPRDARLPAAADLAASARRRPTTMDERTLVPAMLPIVT
                     LNGHSNIPSNLPLQSIAARVVVPRDIQREAYSVVEDHSIPLQVTEMDQRIAVPQNSAP
                     PEPNVKLPVVDPDLVDPDIFMTGEAHLMVSPPSPEKARADLVTRISSVVLHVALIAAI
                     LLQARLFPKHEPTAEEVDIARKQLTLLLPPGAFESSKPSSRPVQRPPAVHVDPRVLRK
                     VAPPVVQPTPAPAPAPVPAKPLKELPSAPIPQKNAVAPEPQTTAPAPKTDTPKPGLKL
                     ETPDTPLPRPNLQLPQSSASRSIQDSLKEATRDSNSGGRAGGVIGPMPRSAPSPGGGR
                     GGGQGAGTYGNGYEILTPTEGVDFSDYMKRVVASVRRNWYAVMPESAQLGDRGRVALQ
                     FRIMRDGSVPTGEPNRLLGSGKEPLDRAAVSAIRSSNPFEPLPPAFSGPYIELRFYFL
                     YNLPLEAAYQ"
     gene            complement(2958..>3341)
                     /locus_tag="VII37_11055"
     CDS             complement(2958..>3341)
                     /locus_tag="VII37_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070325.1"
                     /translation="LEFAGGAVANVTASRVSTERVRKMRFFQQHEYISLDYARRDALR
                     VGVKKPGPQPEFGFEKLPAPAIEPLHAELDAFVESVRSRQEPRVNGAAGRAALELAGR
                     VMTSIHEHAERVQLATRTSAKPVQI"
BASE COUNT          691 a         1105 c          849 g          696 t
ORIGIN      
        1 ccttcgcggc ttcgcgcatg ccgcgcaaaa atatccagga tcagctgcgt gcggtcgatc
       61 actcgccgct ccgtcaactc ctcgatgttt ctctgctgca ccggagaaag attgctgtca
      121 aaaatgatca gcggcgcctg atgggcattg gcctccgcgc gaatctcatc cagcttccca
      181 cgcccgacca gcgtggcgga atccgcggag tcgcgtacct ggaacacagt gccgacaatt
      241 tccgcgccgg cgcttttcgc cagttccact aattcttcta aagacacgcg tccctgctcg
      301 cccgcaggca tccccggaaa ccgaggcgcg cgcttccaac ccacgcccac cagcagcgcg
      361 cgttctgcat tcgaaacatt gtgttgtggt gtcttcgtca aaaagtgcga cctggcggca
      421 ctacgaaacc ttctgcggag gggcgctact catactcgcg attatacgcc ttagcatttt
      481 ccaccgcgac taccggcatc atctgggtat ctgcggagcc cctctcgcaa tctctttgct
      541 aacgtattcg caaatatcgc tttgcacttt gccatcatcg cccggcccag caaaccagcg
      601 aatcgacttg tccttgcgaa accaggtcat ctgccgcttg gcatactgtc gcgtggccaa
      661 ttgaatcgcc gcacgcgctt tctccatcgt aagatcccca cgaagtaccc caagcatttc
      721 gcgatacccg atgaaatcaa aaatctttgc gttctccgtg atccccgccg taagcaatcc
      781 ccgaacctca tccagccagc cactttcgat catcgcctcc gtgcgcgcat caatccgctc
      841 acaaagcatt tcgcgcgaag gcatcagtcc aacctttaac gtccgccatc cttgcaacgg
      901 gctccgcccg gcgcgatgaa cctcagaaat tggcttgcgt gtcagaatgc aaacttctat
      961 cgcgcgtatc aacttttgtt catccgtcgg cgcaattttt gccgcggctt ccgcatccat
     1021 ccgtcgcagc acaccatgca ggtgcatcgc accgcctttc ctcgagctcg cccgcaaccg
     1081 ctcgcgcaat tcctccgaac gcagcggcaa gtccgctaag ccctccagca gtgcgcgcac
     1141 ataaagccca gtccccacgg taagaatcgg cagcctgccg cgcttacgca tatccgccaa
     1201 aactcccagc gccagatcgc gataacctcc cgccgtcgcc gcctccgaag catccagcac
     1261 atcaatcagg tgatgaacga cgccttgccg ctcctctaca cttggcttgg ccgtcccgat
     1321 attcacgccg cgataaacct gcgtcgaatc gcaagcaata atttctgcct gcgtctgcaa
     1381 gacgttcgcc agcgccatcg ccagcgcgga tttgcccgat gccgttggtc ctaccacgac
     1441 aatcaaaggc aaactttcca actcaattgt gttcatggaa aaaaaatgtg ccgcgcgcga
     1501 aacaacagca ccagcagcac caggaacgcg agcaccagcg agcccacgat ttgttgtcgc
     1561 aacggtttca ccggcttttc actgatacgc ggcttcgaga ggcaaattgt acaggaagta
     1621 aaatcgcagt tcgatatacg ggccgctgaa ggcggggggc aaaggctcaa atggattcga
     1681 cgaccggatc gccgaaaccg ccgcgcggtc cagcggctct ttcccggatc caagcaatcg
     1741 gttaggctcc ccggttggca cactgccatc gcgcatgatg cggaattgca gcgcgaccct
     1801 tccgcggtcg cccaattgcg ctgactccgg catgaccgca taccaattgc gtcgcacgct
     1861 cgcgacaact cgcttcatgt aatcgctgaa atccacgcct tccgttggcg tgagaatttc
     1921 ataaccgttt ccataggtcc ccgcgccttg gccgccacca cggcctccgc ctggcgaagg
     1981 agcagaacga ggcatcggac caatcacccc acccgcgcgc ccgccactat tcgaatctct
     2041 cgtggcttcc ttcagcgaat cctgaatcga gcggctggcc gaactctgcg gcaattgcaa
     2101 attgggacgc ggtaatggcg tgtccggagt ttccagttta agacctggct tgggggtgtc
     2161 cgttttcggc gcaggcgccg tcgtctgcgg ttccggggca acggcatttt tttgtggaat
     2221 tggcgcgctt ggcaattcct ttagaggctt cgcaggaacc ggcgcgggcg ccggcgcagg
     2281 agttggctga acaaccggcg gcgcaacttt tctcagcacc cgcggatcca catgcactgc
     2341 tggcgggcgc tgcaccggtc gcgacgatgg cttcgatgat tcgaacgctc ctggcggcaa
     2401 caacaaagtc aattgcttcc gcgcaatatc cacttcctcg gccgtcggct catgtttcgg
     2461 aaataatctc gcctgcaaca aaattgccgc gatcagcgcc acgtgcagca cgactgaaga
     2521 aatccgcgtg accagatccg cccgcgcttt ttctggtgac ggcggcgaaa ccatcaaatg
     2581 cgcttcgcca gtcatgaaaa tatccggatc gaccaaatcc ggatccacca ccggcaactt
     2641 cacgtttggt tcgggaggcg ccgagttctg cggcaccgca atgcgctgat ccatttccgt
     2701 cacttgcaac ggaatcgaat gatcctccac caccgagtat gcttcgcgct ggatatcgcg
     2761 cggcacaact actcgtgcag cgatggattg caacggcaga tttgaaggaa tgttggaatg
     2821 cccgttcagc gtgacgattg gcagcatcgc tggcaccagc gtgcgctcgt ccatcgtcgt
     2881 gggccgccgt cgcgcgctcg ccgctaaatc cgctgccgcg ggtaaacgcg cgtcccgggg
     2941 tacaagtgtg cgttcaatca tatttggact ggcttagctg atgttcgggt agccagttgg
     3001 acgcgctccg cgtgttcatg gatgcttgtc attactcgtc ccgctaactc cagcgctgcg
     3061 cgccccgccg cgccatttac ccgaggttcc tgccgcgaac gcaccgactc cacaaaggcg
     3121 tccaactccg catgcaacgg ctcaattgcc ggcgccggca acttttcaaa accaaattct
     3181 ggttgcggac cgggtttctt tacgcccacg cgcaaggcat cacgtcgtgc ataatccagc
     3241 gaaatatatt cgtgttgttg gaagaatcgc attttacgaa cgcgctcggt agaaactcgg
     3301 ctcgccgtca cattcgccac cgctccgcct gcaaattcca g
//
LOCUS       DATCBB010001011         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_64366, whole genome shotgun sequence.
ACCESSION   DATCBB010001011 DATCBB010000000
VERSION     DATCBB010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64366"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1456)
                     /gene="gabT"
                     /locus_tag="VII37_11060"
     CDS             complement(98..1456)
                     /gene="gabT"
                     /locus_tag="VII37_11060"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY5070326.1"
                     /translation="MNSIQLRTEIPGPRSRALMARREAAIPRGPYHSTPIFAAKADSA
                     ILEDVDGNHFIDFAGGIGCANMGHRSAPVMAAIREQIEKYLHLCFSVTPYEPYVAVAE
                     KLNALAPGKFAKKTFIVNTGAEAVENSIKIARAYTKRPAVICFNDAFHGRTLLAMSLT
                     SKTNPYKAGFAPFVNDIYRIPYAYCYRCSYNLTYPSCKMTCAHALEDAFKRLVPAESV
                     AAVIVEPILGEGGFVTPPKEFFGIIQEICHKHGIVFIADEVQSGIGRTGKFFAIEHYG
                     VDPDLIVTAKSLGGGLPIAAVTGRAEIMDAPGVGGLGSTFAGNPASCAAALAAIEMME
                     SGELLQRSTSIGKIFEARAREWQKKWPLVGEIRGMGGMCAIELVRNRETREPADAETK
                     EIAHFCYEHGLITITAGTYNNVLRILVPLPITDEQLNEGLQVMEAALAAVSDRAPALS
                     HA"
     gene            complement(1487..>2070)
                     /locus_tag="VII37_11065"
     CDS             complement(1487..>2070)
                     /locus_tag="VII37_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5070327.1"
                     /translation="KKIYSKFVEAMTEKAKRIQLGAPLERGTKMGPLVSKEQYERVSS
                     YLEIGKKEAKVAIGGGRPKQFGKGYYVEPTIFYDVDNNARISREEIFGPVASVIPFDD
                     EAGAIRIANDTPYGLAAAVWTRDIYKAFRVVKAVRAGIVWVNHMQPTYVEAPWGGYKQ
                     SGFGRELGPWGLEEYLETKQVFVNLDETPIAWY"
BASE COUNT          446 a          601 c          543 g          480 t
ORIGIN      
        1 tcgaagcaaa agacgacatc aacaaataag ccgcacagct ccgcggcgca ggcgtcgcga
       61 ccgtggccga acaccatcgc gcggtctaat actcaagtta ggcgtgcgac agcgcaggag
      121 cgcgatcgga cacggccgct agcgccgctt ccatgacctg cagaccctca ttgagctgct
      181 cgtcggtaat cggcagcggc actagaatgc gcaacacgtt gttatacgtg ccggctgtga
      241 tggtgatgag gccgtgctcg tagcaaaaat gcgcgatctc tttggtctcg gcatccgcgg
      301 gttcgcgcgt ttcacggttg cgcaccaatt cgatcgcgca cattccaccc atgccgcgaa
      361 tttcgccaac cagcggccat ttcttttgcc actcgcgtgc gcgagcctca aatatttttc
      421 cgatcgaggt agaacgctgt aacagctcgc cactctccat catttcgatg gccgccagcg
      481 cagcggcaca cgacgcagga ttgcctgcga acgtgctgcc aagcccgccg acacccggtg
      541 catccataat ttctgcgcgc ccggtaaccg ccgcgatagg caatcctccg cccagcgatt
      601 tcgcggtgac gatcaggtcg ggatccacac cgtaatgttc aatggcaaag aattttccag
      661 tgcgcccaat gcctgattgc acttcatcgg cgatgaaaac aattccatgt ttgtggcaga
      721 tctcctgaat gatgccgaaa aattctttgg gcggggtcac aaaaccgcct tcgccgagaa
      781 ttggttcgac aatcacggcg gccactgact ccgcaggcac cagccgtttg aaggcatctt
      841 cgagtgcatg cgcgcaggtc atcttgcatg acggataagt cagattgtac gagcagcggt
      901 agcagtaagc gtaaggaatg cgataaatat cgttcacgaa tggcgcgaat cccgctttgt
      961 atggattcgt tttgctggtc agcgacatgg ccagcagcgt gcgcccgtga aacgcgtcgt
     1021 taaaacagat caccgccggc cgtttggtat aggcgcgggc gatcttgatg gaattttcta
     1081 ctgcttccgc gccagtgttc acgataaatg ttttcttcgc gaatttcccc ggtgccagtg
     1141 catttaattt ttccgccact gcaacgtaag gctcataggg cgtcacgctg aagcatagat
     1201 gcaaatattt ttcgatttgc tcgcgaatcg cggccatgac cggagcgctg cgatgcccca
     1261 tattggcgca accgatgccg ccggcgaaat cgatgaagtg gttgccgtct acgtcttcca
     1321 aaatggcgct gtccgcttta gccgcaaaaa tcggcgtgga atgataaggc ccgcgcggga
     1381 tcgctgcttc acgtcgcgcc atcaacgcgc gtgaacgcgg cccgggaatt tctgttcgaa
     1441 gctgaatgga gttcatgttc ttttcctttg aagctaataa cagcgcctaa taccaggcga
     1501 ttggcgtttc atcgagattg acgaacacct gcttggtttc cagatattct tccagacccc
     1561 acggccccag ttcgcggccg aagccagatt gtttgtagcc gccccacggc gcttccacgt
     1621 aagtaggctg catgtgattg acccagacaa ttccagcgcg taccgctttc accacgcgaa
     1681 acgctttgta aatgtcgcgt gtccacaccg cggcagccaa tccgtaaggc gtatcgttgg
     1741 cgatgcggat cgctccggct tcgtcatcga aaggaattac cgaagccacc gggccaaaaa
     1801 tttcttcgcg cgagattcgc gcattgttat cgacatcata aaaaatggtg ggctccacgt
     1861 agtaaccttt gccaaattgc tttggccggc ccccaccgat ggccactttg gcttctttct
     1921 tgccaatctc cagataagag ctgacgcgct cgtattgctc cttgctgacc agcggaccca
     1981 tttttgtgcc gcgttcgagc ggcgcgccca actggatgcg tttcgctttt tcggtcatgg
     2041 cttccacaaa cttggaataa attttctttt
//
LOCUS       DATCBB010001012         1281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_258053, whole genome shotgun sequence.
ACCESSION   DATCBB010001012 DATCBB010000000
VERSION     DATCBB010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258053"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VII37_11070"
     CDS             complement(<1..708)
                     /locus_tag="VII37_11070"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5070328.1"
                     /translation="MSLDSSQQDSKGEMPLRQNAADKALPSHAMQPLEKKWRAGVLGA
                     TGLVGQRIVKLLSNHPWFELTEVAASERSSGKRYSEAVRWHLDGSIPENVRDLIVKDL
                     DPTLDCDFVFSALDSSVAGGAEEDFARAGYPVVSNCKNHRMDPYVPLLIPEVNAAHLE
                     AIPTQQKKCGYDRGFIVTNPNCSTAGLVLVLKPLADAFGLEKVFVVTLQAVSGAGYPG
                     VATMDIQGNVIPFISGEE"
     gene            1091..>1281
                     /locus_tag="VII37_11075"
     CDS             1091..>1281
                     /locus_tag="VII37_11075"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5070329.1"
                     /translation="MSVTKSGQMNALRKMRAGTGLSFESAPVPNVGPTDVLVRVTAAS
                     ICGTDLHIYGWDRWSQGRI"
BASE COUNT          280 a          359 c          356 g          286 t
ORIGIN      
        1 ttcttcgccg ctgataaagg gaatgacgtt gccctgaatg tccatggtcg ccacgcctgg
       61 ataacccgcg ccagacaccg cctgcagtgt gaccacaaaa actttctcca ggccaaacgc
      121 gtcggccagg ggtttcagca ctaaaaccag accggccgtc gagcaattgg gattggtgac
      181 aataaaaccg cgatcgtacc cgcatttctt ttgctgggtc ggaatggctt ccagatgcgc
      241 ggcgtttact tccgggatca gcagcgggac atatggatcc atgcgatggt ttttgcaatt
      301 gctcaccacc ggataaccgg cgcgcgcaaa atcttcttcc gcgccgccag cgaccgatga
      361 atccaacgcc gaaaatacaa agtcgcaatc cagcgtaggg tccagatctt tcacgatcag
      421 atcgcggacg ttctctggaa ttgatccatc gagatgccac cgcacagctt cggaatatcg
      481 cttaccggac gatcgttccg acgcggccac ctcagtaagc tcgaaccacg ggtgattgga
      541 aagcagcttg acgatgcgct gccccaccag accggtcgcg cccagaacac cggcgcgcca
      601 ctttttctca agcggctgca tggcatggga aggcaaagct ttatccgcgg cgttctgcct
      661 caaaggcatc tcgccttttg agtcttgttg ggaactatcg aggctcatgt ctcggctcct
      721 aatttcgccg gagcgttctg gatgcgggtc taaagaaaaa acccaccgcc aggcgcttcc
      781 ggcggtgggt tggaaacttt tgctatttat ctagcggttt ctccactcgc ccgaagcgca
      841 attagtcggc gtcatagtcg acgtgacggt agtaattcgc gtgattcgat tcgtggcgat
      901 ggccatcata gcggggagag aatagcacgt ccgatatcgc tcttaaagcg cttagtgacg
      961 agggtgtaat ctaagatcaa tgcttgtcgc gcgccacgcg accggctcgg ccgcgagttc
     1021 gcgccaatga gccaccatcg tgtagtgtgc taccatccaa aagttacgtt gtacctggga
     1081 gccgcgatag atgtccgtga ctaagtctgg gcaaatgaat gccttgcgca aaatgcgagc
     1141 cggtactggc ttgtcgtttg agtccgcgcc ggtgcccaac gttggcccta ctgatgtcct
     1201 cgtacgggta acggcggctt caatttgcgg cacggatcta catatatatg gatgggatcg
     1261 ctggtcgcag gggcgcatta a
//
LOCUS       DATCBB010001013         1263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_291241, whole genome shotgun sequence.
ACCESSION   DATCBB010001013 DATCBB010000000
VERSION     DATCBB010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291241"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VII37_11080"
     CDS             complement(<1..440)
                     /locus_tag="VII37_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5070330.1"
                     /translation="MSTEVSVPLVHAGETGAPHLRRVLGRWDLVLLFVVAVFNLNVVP
                     SIAANGGVTVWLWLISLVLFFWPQGIAVIELAHRFPGEGGVYLWAKEVFGDFHGFLSG
                     WCYWTNNMMYVPTVMLYFVGVSVYALGAGHESLADNKMFALSASV"
     gene            complement(538..>1263)
                     /locus_tag="VII37_11085"
     CDS             complement(538..>1263)
                     /locus_tag="VII37_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5070331.1"
                     /translation="ILEPILMNVGLCMPQPGFLEGLREIATKNGALLIFDEVKTGAKL
                     AWGGASEFFGVKPDMICLAKSIGGGAPLGAFGASRAVMDLISQHKVFHGGTYNTNRIS
                     MAAGLATFREVLTRENYAHVGKLSKKLTEGYRKTVAKVGLQAYVVSAGVNGALMLYPQ
                     EIRNYRDWTAIDVDLWRHYWFAMVNRGVLAQPYWWDEQWTISVQHTEADIDKHLAVFE
                     EIAPALAQAQQERGVAFVGAAGH"
BASE COUNT          312 a          407 c          304 g          240 t
ORIGIN      
        1 accgaggcgc tcagcgcgaa cattttatta tcggccagcg actcgtgccc cgctcccagc
       61 gcgtacaccg aaacacccac gaaatacagc atcaccgtgg gcacatacat catgttgttg
      121 gtccagtagc accagcccga caaaaaacca tggaagtcgc caaaaacttc cttggcccat
      181 aaataaacgc cgccttcacc gggaaaacgg tgcgccaact caatcaccgc gattccctgc
      241 ggccagaaaa aaagcaccaa cgaaatcagc cataaccaca cggtgactcc gccgttggcc
      301 gcgatggaag gaaccacatt cagattgaaa accgcgacga cgaaaagaag aaccaggtcc
      361 cagcgtccta aaacacgccg caaatgtgga gcgccggttt cacccgcatg gacaagcggc
      421 acgctgactt ccgtcgacat aatttccccg gacgtttgat gatggtgcgt caagctctca
      481 gcgtgcctgc gggtagcttg ctgcccaaaa tcattcgcaa caatttagct caatgcgtta
      541 atgcccggcg gcgcccacga acgcgacacc gcgctcctgc tgcgcctgag ccagcgccgg
      601 cgcaatttcc tcgaacaccg caagatgctt gtcgatatcc gcttcagtat gttgcaccga
      661 gatggtccac tgctcatccc accagtaagg ctgcgccagc acgccgcgat tcaccatcgc
      721 aaaccagtag tgacgccaca aatcaacatc aatggccgtc caatcacggt aattgcgaat
      781 ttcctgcggg tacagcatca gcgcgccgtt tacgccggcg ctgactacgt aggcttgcaa
      841 acctactttg gccaccgttt tgcgatagcc ctcggtgagt tttttgctga gcttgccgac
      901 gtgcgcgtaa ttttcgcgag tcaaaacttc gcgaaaagta gccaggccag cagccatgga
      961 aatgcggttg gtgttgtagg tgccgccatg aaaaactttg tgctgcgaaa tcagatccat
     1021 caccgcgcgg ctggcgccaa acgctccgag tggcgcgcca ccgccaatgg atttagccag
     1081 gcagatcatg tccggcttca cgccgaaaaa ttccgacgcc ccgccccacg ccagcttcgc
     1141 tccggttttc acttcatcaa aaatcagcag cgcgccgttc ttcgtggcaa tctcccgcag
     1201 cccttccaga aatcccggct gcggcatgca taaacccacg ttcatcaaga tcggctcaag
     1261 aat
//
LOCUS       DATCBB010001014         1010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_421539, whole genome shotgun sequence.
ACCESSION   DATCBB010001014 DATCBB010000000
VERSION     DATCBB010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421539"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(526..>1010)
                     /locus_tag="VII37_11090"
     CDS             complement(526..>1010)
                     /locus_tag="VII37_11090"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070332.1"
                     /translation="TLSHKMGMAIAPFFVAAILSGGIIAGFGWFMPARTGDGAKALAG
                     VLGFEDFLTHVEADHMNRISQTPETFEKFLPYAMALGVEKKWVGAFQNIYTQPPSWYQ
                     GGYSSGGFYPIMFVNSLDNMTMRASSVMASAPRSSGGSGFGGGGFSGGGMGGGGGGGF
                     "
BASE COUNT          243 a          309 c          249 g          209 t
ORIGIN      
        1 aaaaacgacc acgtggggat tgctccaatc gaactttccc acagtcccat gcagcgtaat
       61 cgtcttcgtc atgtcatatc cagccacgcc gtggtgcgcc atcacggagc ctgaaaaaat
      121 cacaaggctc gcaaccgcgg ccaggacgac gagcagcttc ctattcatga agcgcctccc
      181 atcaattgcc tgccaatgca tttggaagtc gaatgacggg cgccatcata gcaggaccgg
      241 ggccttttag aaataattcg gctcaaggat aaaatataca agtatccagc cgtgcgggtc
      301 ctggtggccc ggttcttccg ctttcattca ggcggcagcg tgccaatacg cgggacaggc
      361 ttcacagctt gcggaaaaag tatttatttt atcattccga gcgaagcgag gaatctctcc
      421 ttggtttaag cccaacaaca gaaagagaga ttcctcgctt cgctcggaat gacaaaaggt
      481 gggggccttt ttccgcagcc tgtgaagcct atcctactct agcggctaga acccgccgcc
      541 cccaccgccg cccatcccgc cgcctgaaaa tccaccaccg ccgaaacccg aaccgccgct
      601 gctccgggga gcggaagcca tcaccgaact tgcccgcatc gtcatattgt ccagactatt
      661 gacgaacatg attggataaa atccgccgct ggagtagccg ccctggtacc acgagggcgg
      721 ctgcgtataa atattctgga atgcgccgac ccattttttt tccacgccca gcgccatcgc
      781 gtagggcaga aacttctcaa aggtttccgg agtctggctg atccgattca tgtgatcggc
      841 ttcgacatgc gtcagaaaat cctcgaagcc cagtacgccc gctagtgcct ttgctccgtc
      901 gccagtgcgc gccggcatga accaaccaaa cccggcaatg attccccctg aaagaattgc
      961 cgcgacgaaa aacggtgcaa tggccattcc cattttatgc gagagcgtgt
//
LOCUS       DATCBB010001015         3534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_64493, whole genome shotgun sequence.
ACCESSION   DATCBB010001015 DATCBB010000000
VERSION     DATCBB010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64493"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VII37_11095"
     CDS             <1..330
                     /locus_tag="VII37_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5070333.1"
                     /translation="SISKTLFADLEPLRSHPNVGDIRGMGLLIGIEFVSDKKSREPFP
                     QSQNIAERIRQAALEENVLTYPTQGCVDGLRGDHILLAPPFTVSTEESALIARAIKSA
                     LANVFPF"
     gene            451..1668
                     /locus_tag="VII37_11100"
     CDS             451..1668
                     /locus_tag="VII37_11100"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY5070334.1"
                     /translation="MRRNSKIPVSLFAAVLLFTAVVRAQGKDDDKTQTRTQGKPRARD
                     LGVPFDGTPGPLNAITDVAGVTVGHTTLISGEGKLQIGKGPVRTGVTAVLPRGQQSMS
                     NPVFAGWFTENGNGEMTGTTWVEESGFLEGPVMITNTHSVGVVRDSVIQWRVAHGQPD
                     PAGYWWSLPVVAETWDGWLNDINGFHVKSADAFHAIDSASPGPVTEGNVGGGTGMVCN
                     EFKGGIGTSSRKLDAKVGGYTVGVLVQCNYGSRPNLRIAGVPVGKEIPEDRAYASSTF
                     DELDRGSIIVVVATDAPLLAHQLKRLARRVTLGLGRDGSISGNGSGDIFIAFSTANPG
                     AAFADHVVDLKMLPNEKIEPIFAATVQATEEAIINAMVAAETMTGIENHKVIALPHDR
                     LRAVLKKYNRLNE"
     gene            complement(1729..2928)
                     /locus_tag="VII37_11105"
     CDS             complement(1729..2928)
                     /locus_tag="VII37_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY5070335.1"
                     /translation="MPVGTAFHERTLALCESLNYREWSGYYTVSVYEMHHEHEYNAIR
                     NSAALIDISPLFKYKVTGKDATKFVNRVISRDINKVAIDQVIYCCWCDPEGKVIDDGT
                     ITRLAENEYRWTAADPSLRWFQQNALGLSLKIEDITEQLAALALQGPTSGRLLNAVAE
                     ADVTNLKYFRVTHGQIAGVPVDISRTGYTGDLGFEIWMPWRDAVKVWDELNSKGKAFD
                     IHPAGMIALDIARIEAGLILIEIDYTSSKKALIDAQKFSPAEIGLGKLVDLKKETFVG
                     REALVHEAKKGADRALVGLEINWNEVESLYDKVGMAPQVPSMASRTAVPVYRGGRVVG
                     KATSTTWSPTLKKMIALACVDRQNSAADTTLNMEITVEAVRHTVSAKIVPLPFFNPPR
                     KMAVPII"
     gene            complement(3083..>3534)
                     /locus_tag="VII37_11110"
     CDS             complement(3083..>3534)
                     /locus_tag="VII37_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5070336.1"
                     /translation="YAYWRQELPGYDLTAGTFGENFTTEGLLEDAVHIGDRFSIGSAE
                     VVVTQPRLPCYKLGIRFQSDAMVKRFLASKRSGFYLAVTREGEVGAGDEIKEISRDPN
                     HVSISEIIRLYLAKEYNDADIEVVRRALQVTSFPESWKEDFRATLMS"
BASE COUNT          822 a         1016 c          871 g          825 t
ORIGIN      
        1 tcaatttcaa agactttatt tgcagatctc gaacctttgc gctcacatcc gaatgttggc
       61 gatatccgcg ggatgggctt attaatcggc atcgagtttg taagcgacaa aaagtctcgc
      121 gaaccgtttc cgcaatcgca aaacattgcc gaacgcattc gccaagctgc gctggaagaa
      181 aacgtgctca cctatcccac gcaagggtgc gtcgacggcc tgcgtggcga tcacattctt
      241 cttgcaccgc catttacagt gagcacagaa gaatccgcac tcatcgcacg ggcaattaaa
      301 tcagcactgg cgaacgtatt tccattttaa tctcaagatt ttgcgagcaa tcgttattct
      361 aatttacctg gaatccttct tctctttcac cgggccttgc attactgttt caaccttgag
      421 tttcctcact tgtggagata actgttgatc atgcgccgta attctaaaat cccagtctcg
      481 ctttttgccg cggttttatt attcaccgcg gtcgttcgcg ctcaaggtaa agatgacgat
      541 aaaacccaaa caagaactca aggcaaacca cgcgcacgcg atctaggcgt cccttttgac
      601 ggtacccccg gtccactcaa cgccatcacc gatgttgccg gcgtaaccgt cggacacacc
      661 acgcttattt ccggtgaagg aaaattgcag atcggcaaag gccctgtgcg taccggggtc
      721 actgccgttt tgccgcgagg acagcagtcc atgagcaacc ctgttttcgc cggctggttt
      781 acggaaaacg gaaacggaga aatgactggc acgacatggg tggaagagtc cggttttctc
      841 gaaggcccgg tgatgatcac caacacgcac agcgtcggcg tcgtacgcga ttccgtaatt
      901 cagtggcgcg ttgcgcacgg ccagccggat cccgccggat attggtggtc gctgccggtt
      961 gtagcggaaa cgtgggacgg ctggctcaat gacatcaacg gttttcacgt aaaatccgcc
     1021 gatgcttttc acgcgatcga ttccgcatca cccgggccag tgacggaagg caacgtcggc
     1081 ggtggcaccg gaatggtgtg caatgaattc aaaggcggca ttggcacctc atcgcgaaaa
     1141 ctcgatgcga aagtcggcgg ctacacagtc ggtgtgctgg tgcagtgcaa ctatggatcg
     1201 cgcccgaacc tgcgcatcgc cggcgtcccg gttggcaaag aaattcccga ggatcgcgcc
     1261 tacgcatcca gcacatttga cgaactggat cgtggttcca ttattgtggt cgtggccacc
     1321 gacgcgccac tgctggccca tcagttgaag cgcctggctc gccgcgtcac gctgggcctt
     1381 ggccgcgatg gaagtatttc cggaaacggt tccggcgata tttttatcgc gttttcaacc
     1441 gccaaccccg gcgccgcttt tgcggatcac gtggtcgatc taaaaatgct tcccaacgaa
     1501 aagattgagc cgatcttcgc tgccaccgtg caagccaccg aagaagccat catcaacgcc
     1561 atggtcgcgg ccgaaaccat gaccggcatt gaaaatcaca aagtgatcgc cctgccgcat
     1621 gaccgcctgc gtgcggtatt gaaaaaatac aatcgcctga acgagtgaac agggttaatg
     1681 ggaatttcgg aaggcgctcc gatcttaaac gcgcttaaac gcgcggtgtc aaataattgg
     1741 tacggccatc ttgcggggcg gattaaaaaa cggaagcggc acgatcttcg ccgacaccgt
     1801 atgccgaacc gcttccacgg tgatctccat gttcagcgtc gtatcggcag cggagttctg
     1861 gcgatccacg caagccagcg caatcatttt ttttagcgtc ggcgaccacg ttgtggacgt
     1921 cgccttgccg accactcggc caccgcgata aaccggcact gccgtgcgag aagccatgct
     1981 cggcacctgc ggtgccatgc cgactttgtc gtagagcgac tcaacttcat tccaattaat
     2041 ttcgagcccc accagcgccc ggtccgcgcc tttcttagct tcgtgtacca gcgcttcgcg
     2101 gccgacgaaa gtctctttct tcaagtccac cagcttgccc aaaccaattt ccgcgggaga
     2161 aaatttctgc gcatcgatca acgctttctt actggaggtg tagtcgatct caatcagaat
     2221 tagtcctgct tcaatgcgcg cgatatccag cgcgatcatc cccgctggat gaatatcaaa
     2281 cgctttgcct ttggaattta attcgtccca aacctttacc gcgtcgcgcc agggcatcca
     2341 gatttcgaat cccagatccc cggtatagcc ggttcgcgaa atgtccacgg gcaccccggc
     2401 gatttgtccg tgggtcacgc gaaaatattt cagattggtg acatctgctt cggctacggc
     2461 attcagcaac cttcccgacg tgggcccttg cagggcgagt gccgccagct gttcggtaat
     2521 gtcctcgatc tttaaactca agcccagcgc gttttgctga aaccaacgca agctgggatc
     2581 cgctgccgtc cagcggtatt cgttttcggc cagccgcgtg atcgtgccgt cgtcgataac
     2641 tttgccttcc gggtcgcacc aacagcagta aatcacctga tcgatcgcca ccttgttgat
     2701 atcccgtgaa atcacgcgat tcacgaattt cgtcgcatct tttccagtta ctttgtattt
     2761 gaacaacgga gaaatatcga ttaacgccgc ggaattgcgg atggcgttgt actcgtgctc
     2821 gtggtgcatt tcataaacgc tgaccgtgta gtagccggac cattcgcgat aattcaggct
     2881 ctcgcacaac gcgagcgttc tctcatgaaa tgcagtacca accggcacaa accctccagt
     2941 gatcgcggcg caaattacgc gctgcggaaa aacatagcat cttataaaac tatcgaatta
     3001 taggtgttgg ttgagcgcag ccgcaaggcg cttgctccgt agtttttcat ttcgctagtg
     3061 aaattcaagc cccaaatgcg atttaactca ttaacgtggc gcgaaaatct tccttccagc
     3121 tttctggaaa ggacgtgacc tgcaacgcac ggcgcactac ttcaatgtca gcatcgttat
     3181 actctttcgc gagatacaaa cgaataattt ctgaaattga gacgtgatta ggatcgcgcg
     3241 aaatttcttt gatctcatca cctgcgccga cctccccctc tctcgttaca gcaagataaa
     3301 agcccgagcg tttgctggcc agaaaacgct tcaccatcgc atccgactga aaccggatgc
     3361 ccaatttgta gcaaggcagg cgtggctgcg tcaccacaac ctccgcggag ccaatagaaa
     3421 aacgatcgcc gatatgaacc gcatcttcga gcaatccttc ggtcgtaaaa ttctccccaa
     3481 aagttcctgc cgtcaaatcg tatcccggaa gctcttgtct ccaatacgca taat
//
LOCUS       DATCBB010001016         1194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_291274, whole genome shotgun sequence.
ACCESSION   DATCBB010001016 DATCBB010000000
VERSION     DATCBB010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291274"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /gene="gmd"
                     /locus_tag="VII37_11115"
     CDS             complement(<1..1039)
                     /gene="gmd"
                     /locus_tag="VII37_11115"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY5070337.1"
                     /translation="MTKRMALITGITGQDGSYLAEILLEKGYDVHGVKRRSSSFNTGR
                     IDHLYRDPHVPNTHFSLHYGDLTDATNLIRIMQEVQPTEIYNLAAQSHVQVSFETPEY
                     TANADALGTLRLLEGIRILGLKDSSRFYQASTSELYGNAQETPQTEKTPFYPRSPYAA
                     AKLYAYWITVNYREAYGFHASNGILFNHEGPRRGETFVTRKITQAVAAIHLGKQECLY
                     LGNLNAQRDWGHARDFAEGMWLILQQPKPDDYVLATGENHSVREFVELAFAEVGITIQ
                     WQRAGAKECGLCKKTKRKLVQVDVRYFRPTEVEVLCGNPAKARAKLGWKHRVGFRELV
                     REMMADDLKLIS"
BASE COUNT          262 a          325 c          320 g          287 t
ORIGIN      
        1 cggagattag ttttagatcg tccgccatca tttcgcgcac gagctcccgg aaaccgaccc
       61 gatgtttcca acccagcttg gcgcgcgcct tggccggatt tccacacaaa acttcgactt
      121 ccgtcggccg aaaatagcgt acgtcaacct gcaccagctt gcgtttagtc tttttgcaga
      181 gcccgcattc tttcgcgccg gcccgctgcc attggatagt aatgccgact tccgcgaagg
      241 ccaattcaac gaattcgcgc acggaatggt tttcgccagt cgccagaaca taatcgtcgg
      301 gcttgggctg ctgcagaatg agccacatgc cctcggcaaa atcgcgcgca tgcccccaat
      361 cacgttgagc gtttagattg ccgagataga gacattcttg tttgcctaaa tggatagcgg
      421 cgactgcctg cgtgatttta cgcgtcacga aagtctcacc gcgccgcgga ccttcgtggt
      481 tgaacaggat gccgttggac gcatgaaaac cgtaggcttc gcgataattg acggtgatcc
      541 aataggcata gagctttgcc gccgcatacg gactgcgcgg ataaaatggc gttttctcgg
      601 tctgtggggt ttcctgcgcg ttgccgtata gttcggacgt tgaagcctga tagaaacggc
      661 tgctgtcttt caaacccaaa atgcgaatgc cctcaagcag gcgcaaggta ccgagcgcat
      721 cggcatttgc cgtatattcg ggcgtctcga aactcacttg cacgtggctc tgagcagcga
      781 gattgtagat ttccgtcggc tgcacttcct gcatgatacg gatgagattg gtggcgtccg
      841 tgaggtcgcc atagtgcagg gaaaagtgcg tattgggcac gtgcggatcg cggtaaagat
      901 gatcgatgcg gccggtattg aatgaagatg accgccgttt gacaccgtgg acgtcgtaac
      961 ctttttccaa cagaatttcg gccagatagc tgccgtcctg gccggtaatt ccggtaatca
     1021 atgccatccg ttttgtcatc gtcactccca gaaaataaag ctggtttttg cctaacgcta
     1081 agcgccgccg cgtcctcccg ctgcaagaaa gtctgcataa gcttgcgcca ggccatcgcg
     1141 taattgcgtc ttatgtaccc agccaagttt tctaagttta gacacgtcaa gaag
//
LOCUS       DATCBB010001017         1019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_388234, whole genome shotgun sequence.
ACCESSION   DATCBB010001017 DATCBB010000000
VERSION     DATCBB010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388234"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VII37_11120"
     CDS             <1..751
                     /locus_tag="VII37_11120"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070338.1"
                     /translation="ELRQMVDPPYPYYYLQGIGQTPVVIKGSDFRICERGRHGSEPIV
                     LSVDTALRSGPSHSYNVIQAWAFDGTNHLLREQFRQRCSYEVLEDRFYAMAKHFRCYA
                     AIIEEAANGSSLIRFAEKIGIDVVPVNPSTSKAHRLAEHAAIIRQGRIHLARHLFGEF
                     IFEVENFPRNGSDQIDPCSQYLTHRPPPPRVEQQHPVSIPAAAFASRFPRNAMPSNVN
                     SAAIAYGSQVGNYAPPTGTGWHTLRSSSIGK"
BASE COUNT          220 a          298 c          275 g          226 t
ORIGIN      
        1 ggagctgcgt cagatggtcg acccgccgta cccctactat tatctgcaag gaattggcca
       61 aacgccagtc gtgatcaagg ggtcggattt tcgcatatgc gaaagagggc ggcacggcag
      121 cgaacccatt gtgctgagcg tcgacacggc gctgcggagc ggaccgagcc acagttacaa
      181 tgtgatccag gcttgggcct ttgatggtac caaccatctg ctgcgggaac aatttcgtca
      241 gcgatgcagc tatgaggtgc ttgaggatcg gttctatgcg atggcaaaac acttccggtg
      301 ctacgctgcg atcatcgaag aggcggctaa tggttccagc ctaattcgct tcgctgaaaa
      361 aatcgggatc gacgtggtgc ccgtcaatcc gtccacctcg aaagcacatc gcctcgctga
      421 gcatgctgct ataattcgcc agggccggat ccatttagcg cggcatctgt ttggtgaatt
      481 catttttgag gttgaaaact ttcctcgcaa cggcagcgac caaatcgacc cctgctcgca
      541 gtatttgacc catcggccgc ctcctccacg cgtcgaacaa cagcaccccg tatccattcc
      601 agcggcggca ttcgcttcgc gttttccgcg caatgcgatg ccatcaaacg ttaatagcgc
      661 ggcgatcgca tatggctccc aggtcggaaa ctatgcgcca cctactggga ccggttggca
      721 cacattgcgg tcgagctcga tcggaaagtg aatcaacacc ggaaaaagcc cgcgcatcga
      781 agggcgcgcc acggcgcgct ctttgcgata cctcccgcta ccagccgggt gtccatactg
      841 ctttcaggtc acgagcttcg gttttatctt tcttccggaa agcatctcca tcctcagcag
      901 atcatgttcg cgcgcgtcca ttgaacttgg gaaacagcgc ctgaatggtc ggctctgacc
      961 tagtcaccat tttttgcaat ggggcgcgat ggattgagcg tccaatgatc caccgcatg
//
LOCUS       DATCBB010001018         1544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_129381, whole genome shotgun sequence.
ACCESSION   DATCBB010001018 DATCBB010000000
VERSION     DATCBB010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129381"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VII37_11125"
     CDS             <1..1030
                     /locus_tag="VII37_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5070339.1"
                     /translation="AALIYFTGSKEHNVKLRGRAIDMGYTLNEYAMTTLKSGRRVAGR
                     TEEEIYAKLKLDFIAAELRENRGEIEAAAEHRLPKLVERKDIKGDLQMHTTASDGRHS
                     IEEMAKAARDVGHEYIAITDHSKSLTITNGLDEKRLVEHLKTLRDVNKKKLGIRVFAG
                     SEVDILKDGRLDYTDEFLAQIEVVVCSIHSFFNLERAAMTERMLAAIENPYTQIIAHP
                     TGRMILRRDELDYDMEKILDACAKHGVAMECNSYPDRLDLKDVYLRMCKQRGVKVVIS
                     TDSHNALHLPFIRYGVATARRGWLEKSDVINTLPAEQFLAALRPKPGAAKPSPTDARS
                     EKKTAAKM"
     gene            1123..1446
                     /locus_tag="VII37_11130"
     CDS             1123..1446
                     /locus_tag="VII37_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070340.1"
                     /translation="MKIKWMAVLATTLAFTQLAVAGPFTVYPGATRMPDRGERANPSH
                     AVMYSTPDSYAKVLEYYKKDGKENKAHPMEGATVFDFDGGGGIIVRDVGARGTVIVVD
                     HRTKQ"
BASE COUNT          400 a          382 c          463 g          299 t
ORIGIN      
        1 cgcggcattg atttatttca cgggctctaa agaacacaac gtgaagctac gcgggcgggc
       61 catcgatatg ggctacacat taaacgaata tgcgatgacc acgctgaaat ccggcaggcg
      121 ggtggctggg cgcacggaag aagaaattta cgccaaattg aagctagatt ttattgcggc
      181 ggagttgcgg gaaaatcgcg gagagatcga agccgccgcg gagcatcgcc tgcccaaact
      241 cgtggagcgg aaagacatca agggcgactt gcagatgcac acaacggcca gcgatggccg
      301 gcacagcatc gaggaaatgg cgaaagcggc gcgggacgtt ggccacgaat acatcgcgat
      361 taccgaccat tccaaatctt tgacaataac caatggtctg gacgaaaagc gactggtgga
      421 gcacctgaaa acgctacgcg atgtgaacaa gaagaaactg gggatacgcg tgttcgccgg
      481 gtcggaagtc gacattttga aagacgggcg gctggattac accgacgaat tcctggcgca
      541 gatcgaagtg gtggtttgtt ctatccactc ttttttcaat ctggaacgcg cggccatgac
      601 cgaacgcatg ctggcggcca ttgaaaaccc gtacacgcag atcatcgcgc atccgacggg
      661 gcgcatgatt ttgcggcgtg acgagctgga ttacgacatg gagaagattt tggatgcttg
      721 cgcgaagcat ggcgtagcca tggaatgcaa ttcgtatcca gaccggttgg acctgaaaga
      781 tgtgtacttg cggatgtgca aacagcgcgg cgtaaaagtg gtgatctcca ccgactcgca
      841 caatgcgttg catctgccgt ttattcgcta cggcgtggca accgcgcgac gcgggtggct
      901 ggaaaagtcg gacgtcataa acacgctgcc agcggaacag tttttggcgg cgctgcgacc
      961 caaacccggc gcggcgaaac catcgccgac cgacgcgcgc agcgagaaga aaactgcggc
     1021 aaaaatgtga ccagctgtac agtattaaac actcgcggtc attccagcgt gctaggcttg
     1081 tgcgcgtttc aatacagatg aaccgcagag gcgacgcacg atatgaaaat aaaatggatg
     1141 gctgtgttgg ccacgacgtt ggcatttacg cagctcgcgg tcgcgggccc gttcacggtg
     1201 tatccgggcg caacgcgtat gccagacagg ggcgagcgcg ctaatccgag tcacgcggta
     1261 atgtattcga cgccagactc ctacgcgaaa gtgcttgagt attacaaaaa ggatggcaag
     1321 gaaaacaagg ctcatccaat ggagggtgca acggttttcg actttgacgg cggcggagga
     1381 attatcgtgc gcgatgtggg agcacgtgga acggtgatag tggtggacca tagaacgaag
     1441 cagtgaacac ttccctgccg tccttccttt agtggcgaat attcacccgc aaaaccgcaa
     1501 caattgacta gctactttcc gtcgcaaaat agctgatttt gagt
//
LOCUS       DATCBB010001019         1375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_64718, whole genome shotgun sequence.
ACCESSION   DATCBB010001019 DATCBB010000000
VERSION     DATCBB010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64718"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(563..1027)
                     /locus_tag="VII37_11135"
     CDS             complement(563..1027)
                     /locus_tag="VII37_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEY5070341.1"
                     /translation="MPLIHIMYASSAAPDFREHQIPDLLQKARAANEKQDITGMLLYI
                     GGSFFQLLEGEAAVVDSVYTKIGRDTRHARVTQIIREPILERDFTGWTMGFSTVDPIE
                     AGKLLGENDFFNEASCVTRLDSGRAKTLLAALGKRRWQLERSGMLRALGRKA"
BASE COUNT          291 a          392 c          392 g          300 t
ORIGIN      
        1 gcaatttaaa acgcccatta gccggcgttg atccgcagcg ggcggtcgtg atcggcggct
       61 ctcaggcggc tcccaaactt cttcgcttga tcgagaaact tgaagtcttc atggccgaaa
      121 tactccgctt tccaacaaga aaaggtagag cgaaaccgcg gctaccgccg cagcctcagc
      181 gaagcaccaa gcacgcactg cccgacgtta gcggggcgac taagcccaag ccatacacaa
      241 ttagcgctat taggcaaagc ccaacagcaa gaactggacg actacgacgt ggtgcagaca
      301 ctcgtgtaag cctaccggaa gcagcgagcg tccgctttcc ggcatccatg ccgtcgggct
      361 gactgaccgg agttggccga cctcggccgg cggctgggca agtgcgcgct tttcggtttg
      421 tacttccgcc gttgacccag accggccgat cgtcaatgcc cggtctcggg cagtccaata
      481 actacggtaa tagtccgaaa tgcggctact ctcggcctca agcgcacagc gcgtgcggtc
      541 caatctgcgt cttatcgaaa cgttatgcct ttcggccgag cgctcgaagc atcccactgc
      601 gttcaagttg ccagcggcgc ttaccaagtg cggcaagcag cgttttggcg cgacctgaat
      661 ccaaacgtgt cacgcaggaa gcttcgttga agaaatcgtt ctcgcccagc aactttccag
      721 cctctattgg atcaacggtt gaaaaaccca ttgtccatcc ggtgaaatct ctctctaaaa
      781 tcggttcccg gataatttga gttactctcg cgtggcgtgt atcgcggccg atcttggtgt
      841 agacggaatc aactaccgcg gcttcgccct ccagcaactg aaaaaaggat ccgcctatat
      901 agagcagcat tccggtgatg tcttgctttt cattcgctgc gcgtgccttt tgcaggagat
      961 cgggaatctg gtgctcgcga aagtcaggtg cggccgagct cgcgtacatg atatggatta
     1021 ggggcattgg taatcctcaa gtttgcgatc ggttaacacg gctcgacctg tcgctcgcag
     1081 ggaaggtggc acccgggcgg cgaccgcgcg atggacgcgt tcgtgtccag ctacggcgtc
     1141 gcaggggttt gcgatcccat tcctggctat ctagggttcc acgatactgt tacggctgtt
     1201 tcatgtggta ctttacaaat atccatggaa ttctgaacgc ggcgcgtaga tgcgaggatt
     1261 gtattgggct ggcgctcaaa gtggcgcttc gaacagcggg gacgtatgga acttgtggat
     1321 tgcatctact gcagcgctgc gaccggggcg gaccttgcgc ctgcgcaact ccagg
//
LOCUS       DATCBB010001020         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_452752, whole genome shotgun sequence.
ACCESSION   DATCBB010001020 DATCBB010000000
VERSION     DATCBB010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452752"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..473
                     /locus_tag="VII37_11140"
     CDS             33..473
                     /locus_tag="VII37_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5070342.1"
                     /translation="MDSREKLNLRKTLFISLLALIVLSIVLGVTHRGQWNIPEEAKRR
                     ENPIHSSHEALNAARVLYDDHCERCHGKTGRGDGADATKFSTSPRDLTDTKRMTEQSD
                     GELFYKISEGRRPMPEFKTKLTEEQRWQLVLLMRLMAGGEKKNP"
     gene            complement(551..1024)
                     /locus_tag="VII37_11145"
     CDS             complement(551..1024)
                     /locus_tag="VII37_11145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY5070343.1"
                     /translation="MSLAIGQAAPDFTLKDQSQKDVKLSDFAGKKNVVLVFYPLDWSP
                     TCTNEHACFVNDMRSFEQLDAEVLGVSVDSVWSHKAYADKMGIHYSLLADFNPRGAMA
                     EKYGAYLADKGITNRSIVIINKQGKIAWIKNYDIPVVPDLKEVSAALQQVKSAAA"
     gene            complement(1486..>2179)
                     /locus_tag="VII37_11150"
     CDS             complement(1486..>2179)
                     /locus_tag="VII37_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5070344.1"
                     /translation="GFAIPEGVVASAYPQLVKFGHIHQPAIGALVQTITPELAAALRL
                     KRDFGVIISDISPGGPADTAGIKIQDVILNVDGVPISSLPQFAQSLYLHKTGDQAALE
                     ILRGADRLKINVGLAERPHKEDSLVDLADPVKNLVRPLDILGVELTLELAHSLPDLRI
                     PTGVIVAARTLGTRTGEIPLQTGDVIHGLNGNPITSLDGLRAALAAIKPGEPVALQIE
                     RHEQIFYVSFTR"
BASE COUNT          510 a          595 c          605 g          469 t
ORIGIN      
        1 cgctcgtagt atcctgccac acgaccgaaa ccatggattc gcgcgaaaaa ttgaacctgc
       61 gtaagactct gttcatctct ttgttggcgc tgatcgtctt atcgatcgtg ctgggggtga
      121 cgcacagggg gcaatggaac attcctgaag aggccaagcg gcgcgaaaat ccgattcact
      181 cctcccacga agctctcaat gccgcgcgag tcctgtacga cgatcattgc gaacgctgcc
      241 atggaaaaac cggcagaggc gatggcgccg acgcgacgaa attctccaca tctccgagag
      301 acctgaccga cacgaagcgc atgaccgagc agtcggacgg cgaactcttc tacaaaatca
      361 gcgagggccg gcgcccgatg ccggaattca aaacaaaatt gacggaagaa cagcgctggc
      421 aattggtgct gctgatgcgg ttgatggcag gcggcgaaaa gaagaatccg tagcgataag
      481 aaatggcaac gtcgcggcaa gcccctatag agctatgccg cgcgtcggtc aaactgcaac
      541 gatgagttat ttacgccgct gcggatttta cttgttgtag cgcggcggag acttctttca
      601 gatccggcac caccggaatg tcgtagttct taatccaggc gattttgccc tgcttgttga
      661 tgatcacgat ggaacggttg gtgatgcctt tgtcggcgag gtacgcgccg tatttttccg
      721 ccatggctcc gcgaggatta aagtcagcca gcagcgaata atgaatcccc attttgtcgg
      781 cgtacgcttt gtgcgaccac acgctgtcca cgctgacgcc cagcacttcc gcatccagct
      841 gttcgaacga cctcatgtca ttgacgaagc aggcgtgctc gtttgtgcaa gtaggactcc
      901 agtccagcgg ataaaaaacc agcacaacat ttttcttccc cgcaaaatca gaaagcttca
      961 cgtctttctg actctgatcc tttagtgtga aatccggcgc tgcttgacct atggctaagc
     1021 tcataaatgt tcctctcagt tccaagtatg ccaagcatct tacccaatcg ccgggccgcg
     1081 ttgggtaaaa aatgggttaa catcaaaggg tagctcactt tccgtgatgt gacgggcaaa
     1141 tccatctgga aggagagact accgtgaaga gcaacttttt tctacgagcc cagcagcaaa
     1201 cgccgcaagg ggttcccgtc cgaaactaag gtcaagcgcg gtcagcgtgt tgtccatggc
     1261 gggaaggaac tatctgagaa actgggcagg aatgacctct gcccttgcgg ttcgggacgt
     1321 cggtttcaag aactgttgca tgcgaaccgg ccggtatgac ggtcagccgc gaaactacta
     1381 caacaggtag ataaccacga ggcacggcgc gagccgtgcc ttttggacag tatggggtga
     1441 agggctggtt cggcggctac tcgggttaaa atattttgcg atggcctaac gtgtgaatga
     1501 gacgtaaaaa atctgctcat gccgctcgat ttgcagcgct accggctcgc ccggcttgat
     1561 cgccgccaat gcggcgcgta aaccgtccaa cgaagtgatg ggattgccgt tcaatccgtg
     1621 aattacatcc ccggtctgca acggaatctc cccggtgcgc gtaccgagcg tccgcgcggc
     1681 cacaattacg ccggtgggaa tgcgcaggtc aggcaacgag tgcgcgagtt cgagtgtcaa
     1741 ttcaactccc aggatgtcca gcggcctgac cagatttttt accgggtcgg cgaggtcgac
     1801 gagactatct tctttgtgcg gccgttcggc gaggcccacg tttattttca gtcgatcggc
     1861 tccgcgcaaa atttcgagtg cggcttgatc accggtttta tgcagataaa ggctctgcgc
     1921 aaattgcggc aggctgctga taggcacgcc gtcgacattc agaatcacgt cctgaatctt
     1981 gatgcccgcc gtgtccgcgg gaccacccgg cgaaatatcg gaaataatga cgccaaaatc
     2041 tcgtttcagg cgcaatgccg cggctaattc cggggtgatg gtttgcacca gcgcgccaat
     2101 ggccggctga tgaatatggc caaacttcac caactgagga taagcggacg ccaccacgcc
     2161 ttccggaatg gcgaatccc
//
LOCUS       DATCBB010001021         1469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_452787, whole genome shotgun sequence.
ACCESSION   DATCBB010001021 DATCBB010000000
VERSION     DATCBB010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452787"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VII37_11155"
     CDS             complement(<1..726)
                     /locus_tag="VII37_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5070345.1"
                     /translation="MHRREFLKDTIASGLALAASRRMSAQQKLTDQLAIVDGGGANVV
                     AFSSGDALLLVDSGAPKSGDKVIATLKSWNANAKIQTLFNTHYHLDQTGNNQMFAGAK
                     IIAHERTRQWMSTDYWVPELDRYEKARPKEARPTETFETTGSMTAGGEKIDYGYLLLA
                     HTSGDIYVFFRTANVLAVGDVASPVRDPALDYFTGAWIGGRVDAMDKILALANEQTRI
                     VPAYGPVMTRAELKAERDMLEEVR"
     gene            complement(728..>1469)
                     /locus_tag="VII37_11160"
     CDS             complement(728..>1469)
                     /locus_tag="VII37_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY5070346.1"
                     /translation="EGDTPPERASIRRQTNSILDLILDRTKQLQGELGNSDRSLLEGY
                     LESVREIERRTQKAASKDLSSLKIPEAPIGELDSFPDQVKLMFDLIALAYQADLTRVA
                     SYIMVAEGTNRTYNHIGVPDSFHPVSHHANDMERLNKLVKIQSWHVEKFAEFLAKLAG
                     TPDGPGTLLDHSILMYGSNMSNSDLHNNYPEPNIVVGGGNGKMKLGGQHIVLPERTPI
                     ANLHLTLLQKVGVERDKFGDSTGTIAEI"
BASE COUNT          263 a          421 c          425 g          360 t
ORIGIN      
        1 gcgcacttct tccagcatgt cgcgctcggc cttcaactcg gcgcgggtca tcacggggcc
       61 gtacgccggt acgatcctgg tctgctcatt ggccagcgcg agaatcttgt ccattgcgtc
      121 cactcggccg ccgatccagg ctcccgtgaa ataatcgagc gctgggtctc gcacgggcga
      181 tgccacgtcg cccaccgcta atacatttgc ggttctgaaa aagacgtaga tatccccgct
      241 ggtgtgcgca agcaaaaggt agccgtaatc gattttttcg cccccggcgg tcatgcttcc
      301 ggtagtctca aacgtctccg ttgggcgtgc ctctttggga cgcgcctttt cgtagcgatc
      361 cagttccggg acccaataat cggtggacat ccactgccgc gtgcgctcat gggcaatgat
      421 tttcgcgccc gcgaacatct ggttgttgcc ggtctgatcc aggtgatagt gggtgttgaa
      481 caacgtctgg attttggcat tcgcgttcca gcttttgagc gttgcgatta ctttgtcgcc
      541 ggatttggga gcgccgctat ccaccagcag caacgcgtcg ccagaagaga aagcgactac
      601 attcgcgcct ccgccgtcca cgatggccag ttgatcggtt aatttctgct gcgcggacat
      661 gcggcgtgag gccgccagag ccagtccact cgcgatcgtg tcctttaaga attcccggcg
      721 atgcatgtca gatttccgct atggtacctg tactgtcgcc gaatttgtcg cgctcgacgc
      781 ccactttttg gagcagagtc aggtggaggt ttgcgatcgg tgtgcgttcc ggcagaacga
      841 tatgctggcc gccgagtttc attttgccgt tcccgccgcc aaccacaatg ttcggctccg
      901 gatagttgtt gtgcagatcg ctgttgctca tgttcgatcc gtacatgagg atagagtgat
      961 cgagcagcgt ccccggccca tccggcgtgc cggccagctt cgcgagaaac tctgcgaatt
     1021 tttccacgtg ccagctctgg atcttcacca gcttgttgag tctctccata tcattggcgt
     1081 ggtgcgatac gggatggaac gaatctggca cgccgatgtg gttgtaagtc cggttggttc
     1141 cttccgccac cataatgtag gaggccacgc gcgtgaggtc tgcctgatag gcgagcgcga
     1201 tcaagtcgaa catcaatttc acctgatcgg gaaaggagtc cagctcgccg atcggcgctt
     1261 cgggaatctt caaagacgag agatcttttg acgctgcctt ttgcgtgcgc cgctcgatct
     1321 cgcgaacgct ttctagatac ccttcgagaa gagagcggtc gctattacct agttctccct
     1381 ggagctgttt ggtgcgatcc aaaatcaggt ccaggatact gttggtctgg cggcggatgg
     1441 aggcacgttc cggcggcgtg tcgccctca
//
LOCUS       DATCBB010001022         1731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_129514, whole genome shotgun sequence.
ACCESSION   DATCBB010001022 DATCBB010000000
VERSION     DATCBB010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129514"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VII37_11165"
     CDS             complement(<1..637)
                     /locus_tag="VII37_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY5070347.1"
                     /translation="MSKKLLGIDVGTGGTRVVLVDSDGRVLHSATTEHAPIFAAHIGW
                     AEQEPADWWRAACASIRACLGESKTNPSEITGIGLSGQMHGLVLLGAQGDVLRPSIIW
                     CDQRTDAQCKAITEKIGARRLIELAANPALTGFTLPKIWWVRQFEPENWSRVRSIMLP
                     KDYIRFRLTGARGIDMADASGTLMLDVAGRKWSREILQASNVDEALLPQLFE"
     gene            complement(653..1648)
                     /gene="galE"
                     /locus_tag="VII37_11170"
     CDS             complement(653..1648)
                     /gene="galE"
                     /locus_tag="VII37_11170"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY5070348.1"
                     /translation="MAILVTGGAGYIGSAAVEFLLAKGEKVVVLDDLFRGHKESLSPA
                     AAFYQGSVGDRALVKKIAQEHGVDSCIHFAAMAYVGESVEKPAVYYENNVGQGIDLLG
                     ALLESGVRQMVLSSSCATYGDPPQVPIPENTPQSPTNPYGWTKMIFERVLEAYDHAYG
                     LKFVGLRYFNAAGATENCGEQHEPESHLIPNILAAAAGQRPQVSVFGSTYPTPDGTAI
                     RDYIHITDLADAHYRALQHLRKGSPSDFLNLGTGRGYSILEVIQAAREVTGKEIKTVM
                     QDRRPGDPPRLVADNKKAKTVLGWEPQQSDLRSIIASAWRWHQRNPAGYGTPKGK"
BASE COUNT          351 a          502 c          518 g          360 t
ORIGIN      
        1 actcaaaaag ttggggaagc agcgcttcgt ctacgttcga agcctgaaga atttcgcgcg
       61 accacttcct tccggccacg tccagcatca gcgttccgga agcgtcggcc atgtcaattc
      121 cgcgcgcgcc ggtcagccgg aagcggatgt agtccttggg cagcatgatg gagcgcacgc
      181 gcgaccaatt ctccggctcg aactggcgca cccaccagat tttgggcagc gtgaatccgg
      241 tcagcgcagg attggcggcc aattcaatca gacgccgcgc gccgattttc tccgtgatgg
      301 ctttgcactg tgcgtcggtg cgctggtcgc accagatgat ggagggacgc aacacatcac
      361 cttgggcacc gagcaaaacg aggccgtgca tctgtccgct gagaccgatg ccagtaattt
      421 cgctgggatt tgtttttgat tcgccaaggc aggcgcgaat ggaagcacac gccgcgcgcc
      481 accaatctgc tggctcctgt tccgcccagc caatatgcgc ggcaaaaatg ggcgcgtgct
      541 cggtggttgc ggaatggaga acgcggccgt ctgagtcgac caagacaacg cgggttccac
      601 cggttccgac gtcaatgccg aggagctttt tgctcatgcg cgattgaagg tcttacttcc
      661 ctttcggagt gccgtagccg gccggattgc gttgatgcca gcgccaggcc gatgcaatga
      721 tgctgcgcaa atccgattgc tgtggctccc agcccagcac agttttagct tttttgttat
      781 cggcaacgag gcgcggggga tcgcccggac gacgatcctg catgactgtt tttatttcct
      841 tgccggtaac ttctctggcc gcctgaatca cttccaggat gctgtagccg cggccggtgc
      901 cgagattcag aaaatcggat gggcttccct tccgcagatg ctggagcgcg cggtaatggg
      961 cgtcggccag atcggtgatg tgaatgtaat cgcggatggc ggtgccgtcg ggcgtcggat
     1021 aggtgctgcc aaatacggaa acctgcgggc gttggccggc agcggcggcc aggatattgg
     1081 gaatgaggtg cgactccggc tcgtgctgct cgccgcaatt ttccgtggct cccgcggcgt
     1141 tgaaataacg cagcccgaca aatttcaaac cgtaagcgtg atcgtaagct tcaaggacac
     1201 gttcaaaaat catcttggtc cagccgtagg gattggtcgg cgactgcggc gtgttttcag
     1261 gtatgggaac ctgcggcgga tcgccgtatg tggcgcagga agaggagagc accatctggc
     1321 gcacgccgga ttccagcagc gcgccgagca aatcgattcc ctgcccgacg ttgttctcat
     1381 aataaaccgc gggcttttcg accgactcgc ccacgtaggc catcgcggca aagtggatgc
     1441 aggagtcgac gccatgctcc tgagcgattt tttttaccag cgcgcgatcg ccgaccgagc
     1501 cttgataaaa agccgcggcg ggactgagcg attccttgtg cccgcggaaa agatcgtcga
     1561 gcacaaccac cttttcgccc ttggcgagca aaaactccac cgcagcactg ccgatatacc
     1621 ccgcaccgcc ggtcaccaga atagccattc gtcgcgatta cctttctgcg aaagattcga
     1681 ttcaggactg taacttgtcc gcatggagag tgcgacgcaa attaccacat c
//
LOCUS       DATCBB010001023         1037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_258408, whole genome shotgun sequence.
ACCESSION   DATCBB010001023 DATCBB010000000
VERSION     DATCBB010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258408"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VII37_11175"
     CDS             <1..639
                     /locus_tag="VII37_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5070349.1"
                     /translation="DGRQKAALYSRPSQHQSGSNSASQHLAAFGKVEHADFLNQMLYL
                     DTKIFMTTLNLTYNDKMSMASSVEVRVPFLDRELAEFVAWNVPPRSKLKGKWRPVTKH
                     IFREAMRSMLPPEVMRQPKAGFAAPVDYWLANELRPMVDDLLSESQIRKRGLFRPEVV
                     RRYIDEQRRGTEDWSMQIWQLLTLEIWMQLFLDGGSRSLAQRQIGTKQLATA"
     gene            747..>1037
                     /locus_tag="VII37_11180"
     CDS             747..>1037
                     /locus_tag="VII37_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070350.1"
                     /translation="MQNVKNEKMFRALNEAAALLRAPQRVIPLISRLASDELRTAFVP
                     RHLYSYTATTVPTADALRTIIDWILRAQRADGGIAAYYSFLTGYSASYPEVTG"
BASE COUNT          244 a          289 c          290 g          214 t
ORIGIN      
        1 gatggccggc aaaaggccgc tttatattcg aggccctcgc agcaccagtc cggttcaaat
       61 tctgccagtc aacatctggc ggcgtttggc aaagttgaac atgcggactt tctaaaccag
      121 atgctctatc tggatacgaa aattttcatg acaaccttaa acctgactta caacgacaag
      181 atgagcatgg cttcgtcggt cgaggtgcga gtgccgttct tggatcgcga gcttgccgag
      241 tttgtagcct ggaatgtgcc gccacggtcg aagctaaaag gaaaatggcg tccggtaacc
      301 aagcacatct tccgcgaagc catgcgcagt atgctgccgc ccgaagtcat gcgccagccg
      361 aaagcgggat tcgcggcgcc ggtcgattac tggctggcaa acgagttgcg tcccatggtc
      421 gacgatctcc tttcggagag ccagatccgc aagcggggat tgtttcggcc agaagtggta
      481 cgccggtaca ttgacgagca acggcggggc acggaagact ggtcaatgca gatatggcaa
      541 ttgttaaccc ttgaaatctg gatgcaactg tttctagatg gcggttcgcg ctcacttgct
      601 cagcggcaga ttgggacgaa gcagctagcg accgcataag tgaacgagaa gtggagcgcc
      661 gggcgtcccc gcccggcaaa gacgaacctg tcaggttagc gagatcgaaa gcagaatagg
      721 atttgaatag cctcaagtga agttccatgc aaaatgtcaa gaatgaaaaa atgtttcgcg
      781 cgctgaacga agctgccgcc ctgctgcgcg cgccgcagcg cgtgatcccg ctcatctcca
      841 ggctcgcttc ggacgaattg cgaaccgcgt tcgtgcctcg acacctctat tcgtacacag
      901 ccacaaccgt tcccaccgcg gatgcactgc gcacgataat agattggatt ctccgcgcac
      961 agcgcgccga tggcggcatc gccgcctact acagttttct cactggatac tctgcttcct
     1021 atcccgaagt gaccggc
//
LOCUS       DATCBB010001024         1679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_356078, whole genome shotgun sequence.
ACCESSION   DATCBB010001024 DATCBB010000000
VERSION     DATCBB010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356078"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1581
                     /locus_tag="VII37_11185"
     CDS             40..1581
                     /locus_tag="VII37_11185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5070351.1"
                     /translation="MIAPELILTVVACVALVMEVILPYKLSKWTAYFSLTGIGLALIS
                     LGAQFVNMGGTFNLRALQSLSAMDGFYGMIRIDGFALVFKAIFLIAAALAIAIAMRFL
                     DIEREQHGEYYALILFATVGMMFLGSGYDLIALYIALELMAVTFYVLVAFTKRQKRSN
                     EAGMKYFLLGAFSSGILIYGMSLLYGVTGSTNLGDIGRGVATITASSPMRPMLLLGMI
                     ALAAGLFFKIAAVPFHMWAPDAYEGAPTSVTAFLSTGSKAASFALYARIFFVALAPMQ
                     IDWAPLLGIVAALTIVVGNWAAITQQNSKRLLAYSSISNAGYLLLGVIANNAYGNIGL
                     VIYLLVYTLMNMGAFGVIISLRRRGIIGDNVDDMTGLAQKAPGMAAMMAVFMLSLGGM
                     PGTGGFIGKWYLLYGLFARADADHKNWYYWLAGWAIINLVVSFFYYLRFIRVMYLGDR
                     VADDQPLTMSPALRTALVAALVGILFVGVYPQPFIVLAQKLFPAQTNSNPVRQPSSII
                     DESPK"
BASE COUNT          290 a          463 c          485 g          441 t
ORIGIN      
        1 tttcaaacaa gcgccccgat taatgttcgc gatctgcaat tgatcgcgcc tgagctgatc
       61 cttacggtgg tcgcctgcgt ggccctcgtg atggaagtca ttctccctta caagctgagc
      121 aaatggacgg cctacttctc actcacggga attgggctgg cgctgatctc gttaggggcc
      181 cagttcgtga acatgggcgg gaccttcaac ttacgcgcct tgcaaagcct gtcagccatg
      241 gacggctttt acggaatgat ccgcatcgat ggcttcgccc tggtcttcaa agccatcttt
      301 ttaattgccg cggccctggc gatcgcgatc gccatgcgct tcctcgacat tgagcgcgag
      361 cagcatggcg aatactacgc gctgatttta tttgcgaccg tcgggatgat gtttctcggc
      421 agcggttacg atctgatcgc gctctacatc gcgctcgaat tgatggccgt gacgttctat
      481 gtgctggtgg ctttcaccaa acgccagaag cggtcgaacg aagcggggat gaaatacttt
      541 ctgctgggcg cattttcatc cggcattctg atctacggca tgtctttgct ttatggcgtg
      601 acgggttcga cgaatctcgg cgatattgga cgcggcgtcg ctacgattac agccagctcg
      661 cccatgcggc cgatgctgct gctgggaatg attgctctgg ccgccgggtt gttctttaag
      721 attgcggccg tgccgtttca catgtgggcg cccgatgcct acgaaggtgc gcccacgtct
      781 gtcaccgcgt ttctctcaac cggatcgaaa gccgcgagct tcgcgcttta cgcgcgcatc
      841 ttctttgttg ccctggcgcc aatgcagatt gactgggcgc cgttgctggg gattgtcgcg
      901 gccctgacga tcgtggtggg caactgggcc gcgattacgc agcaaaattc caagcgcctg
      961 ctggcttact cttcgatttc gaacgcgggt tacttgctgc tgggcgtcat cgccaataat
     1021 gcttatggca acatcggcct ggtgatctat ctgctggtgt acacgttgat gaatatgggc
     1081 gcgtttggcg taatcatcag cctgcggcgg cgcgggatca tcggcgacaa cgtcgatgac
     1141 atgaccggcc tggcgcaaaa ggcgccgggc atggcggcga tgatggcggt cttcatgttg
     1201 tcattgggag gaatgccggg cacgggcgga ttcattggca agtggtattt gttatacggc
     1261 ttgttcgccc gcgcagatgc cgatcataag aattggtact attggctcgc tggctgggcg
     1321 attatcaatc tcgtcgtttc tttcttttat tacctgcgtt ttattcgcgt gatgtacctc
     1381 ggcgatcgcg ttgctgatga ccagccgctg acgatgtcac cggcgttgcg gaccgcgctg
     1441 gttgccgcgc tggtcggaat cctctttgtt ggcgtttatc cccagccatt tattgtcctg
     1501 gcccagaaac ttttccccgc ccagaccaac agcaacccgg tacgtcagcc aagtagtatt
     1561 attgacgagt cgccaaagta agtgtttcca acaccggccc ttcttctctt ttatttcttc
     1621 gccgcaatcg caatctggct gggcctggtt tcgctccgcg gcggggtgcg atttgtgcg
//
LOCUS       DATCBB010001025         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_388362, whole genome shotgun sequence.
ACCESSION   DATCBB010001025 DATCBB010000000
VERSION     DATCBB010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388362"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2301
                     /locus_tag="VII37_11190"
     CDS             <1..2301
                     /locus_tag="VII37_11190"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEY5070352.1"
                     /translation="ECYAPNATSRILDNEQRIIKLINNYGMMSFNFGPTLLSWLEAKA
                     PRVYAALQEAEQLSCHKFGSHGSAVAQAYNHMILPLANRRDKVTQVKWGIADFEHRFK
                     RRPEGMWLPETAANTETLEVLAENGILYTIMAPRQAKRVRRKGGRSWKDVTGDRIDPS
                     RAYLTNLPSKKTISVFFYDGPISQGVAFEGLLNDGKRFADRLLSGFADSRDWPQLSHI
                     ATDGESYGHHHRFGEMALTYALQHIENDKLAELTNYGQFLERYPADHFVEIVENSSWS
                     CVHGIERWRSNCGCNSGGHGNWNQEWRAPLRAALDWLRDKVNPLFEEKAPALLKNPWD
                     ARDEYIRVILNRSDASVDAFFNNHATHPLSPEEQVTALKLLEMQRHAMLMYTSCGWFF
                     DELSGLETVQVIHYAGRVVELAKEFLGAEIEPQFLEHLRQAKSNLPEHGDGAQIYEKW
                     VKPAAVNIEKLAGHYAISSLFENYGEKTGIYCYDVDREQYSLEAEGKLRLATGRARFS
                     SEVTRESATMSFGVLHLGDHNVTGGVRRADGPERYEELKTKLIDCFSKADTAAVIRIL
                     DEEFSEHSYSLSSLFRDEQRKIVGLILNDSLTSAAAAYRTIYENQAPLIRFLNSLNIP
                     VPAAFKAAADVAVNSQLRQSFERADLDGDNIQSFLKEAATSRVPLDTTTLEFTMRKRL
                     EEQAARFAERPDDMENVQRFRKQLELTQTLPFPVTLWEVQNISYAPLMKAIEDRRLAA
                     ENNDPEAFAWMKELTALRDGLRILGA"
     gene            2306..>2808
                     /locus_tag="VII37_11195"
     CDS             2306..>2808
                     /locus_tag="VII37_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY5070353.1"
                     /translation="MVSTRIPVATYRLQFNKDFTFNQATGILDYLRELGITDIYASPI
                     LTSRRGSGHGYDVTNPNRLDPDLGSEKDFEVFVSELEKRGMGLLLDIVPNHMAASSEN
                     PWWIDVLENGPDSAFSSYFDIDWHPPSRNLDNKILLPILGRPFGEALDSSELKLNFRD
                     GQFSIEY"
BASE COUNT          693 a          769 c          724 g          622 t
ORIGIN      
        1 gaatgctacg cccccaatgc cacctcgcgc attctggata acgagcaacg catcatcaag
       61 ctgataaaca actatggaat gatgagtttt aattttggcc caacattact ttcctggctg
      121 gaagccaaag cgccgcgtgt ctatgcagca ttgcaagaag ccgagcaact gagttgccac
      181 aaattcggaa gccatggctc cgctgtcgcg caggcgtata accatatgat tttgccgctc
      241 gcgaatcgac gcgacaaagt cacccaggtt aagtggggta tcgccgattt tgagcatcgc
      301 ttcaagcgcc gcccggaagg catgtggctg cccgagactg cggccaatac ggaaaccctt
      361 gaagtgctgg cggaaaacgg aattttgtac accatcatgg cgccgcgcca ggccaagcgc
      421 gtgcgacgca agggcggtcg cagctggaag gacgttaccg gtgaccgcat cgatcctagc
      481 cgcgcttatc tcacaaattt accttccaaa aaaacgatca gtgttttttt ctatgacggg
      541 ccgatctctc aaggcgtcgc ctttgaaggc ttgttgaatg acggcaaacg attcgcagat
      601 cgtttgctta gcggattcgc cgactcgcga gattggccgc agctttcgca catcgctacg
      661 gatggggaaa gctacggaca ccatcaccgc ttcggcgaaa tggccttgac ctacgccttg
      721 caacacatcg aaaacgacaa actcgcggag ctcaccaatt acggccagtt tcttgagcgc
      781 tatcccgccg accattttgt cgaaatcgta gagaacagct cgtggagctg cgtgcacgga
      841 atcgagcgtt ggcgctccaa ttgtggctgc aattcaggag gccacggtaa ctggaaccag
      901 gaatggcgtg cgcccttgcg cgcggcactt gactggctgc gcgataaagt gaatccgctg
      961 tttgaagaaa aagctcccgc gctcttaaag aatccatggg acgcccggga tgaatacatt
     1021 cgggtaatcc tgaatcgctc ggacgccagt gtagacgcat tcttcaataa tcacgcaacg
     1081 catccattaa gtccggaaga acaagtcacc gctttgaagc tattggaaat gcaacgccac
     1141 gccatgttga tgtacaccag ctgcggatgg ttcttcgatg aactttctgg actggaaacc
     1201 gtgcaggtaa ttcattacgc cgggcgcgtc gtcgaattgg ccaaggaatt tttgggcgcc
     1261 gagatcgaac cacaatttct ggaacattta cgccaggcaa aaagcaattt gccggaacac
     1321 ggtgacggcg cgcaaattta cgagaaatgg gttaagcccg cggccgtcaa tatcgaaaag
     1381 cttgcaggcc actacgccat cagctctcta tttgagaatt acggtgagaa gaccggcatc
     1441 tactgctatg acgtcgacag agagcaatat tctcttgagg cggaaggcaa actgcggtta
     1501 gcgaccggcc gagcgcgctt cagctccgaa gttacccgcg aatccgcaac catgagtttt
     1561 ggtgtactgc acctcggaga tcacaacgtt accggaggcg tgcgccgcgc cgatggtccg
     1621 gaacgctacg aagaattgaa aaccaagctc attgattgtt tttccaaagc ggatactgcc
     1681 gcggtgatac gcatactcga tgaagagttc agcgaacatt cctattccct tagttcgctg
     1741 ttccgcgacg aacaaagaaa aattgtgggc ttaatcctga atgattcgct tacttccgcg
     1801 gctgcggcgt accgaaccat ctacgaaaat caggcgccct tgatacgttt tctaaattcg
     1861 ctcaacattc ccgttcccgc ggcctttaag gccgctgcgg acgtggccgt caacagtcaa
     1921 ctccggcaat ccttcgaacg cgccgatctc gacggagaca atatccaaag ttttctgaag
     1981 gaagcagcca ctagccgggt gcccttggat accaccacgc tggaatttac tatgcgcaag
     2041 cgcctcgaag agcaagccgc acgtttcgca gaacgccccg acgacatgga aaatgtgcaa
     2101 cgtttccgca agcaattgga acttacgcaa actttgccat ttcccgtaac gctctgggaa
     2161 gtccaaaaca tctcttacgc cccgctgatg aaagccattg aggaccgccg cttggcggcg
     2221 gagaataacg atccggaagc cttcgcctgg atgaaggaat tgaccgcgct gcgcgacggg
     2281 ctacgtattt tgggcgcgta aatttatggt ctctacccgc attcccgtgg cgacctatcg
     2341 gctgcagttc aacaaagatt ttacgtttaa ccaagcgacc ggaattctcg actacctgcg
     2401 ggagcttggt attacggata tttatgcttc acccattctc accagccgcc gcggcagcgg
     2461 tcacggctac gacgtcacca atcccaatcg cctcgatccc gatttaggca gtgaaaaaga
     2521 ttttgaagtt ttcgtcagcg agctggaaaa acgcggcatg ggattgctcc tggatatcgt
     2581 gcccaaccac atggccgcga gcagtgagaa tccctggtgg atcgacgttt tggagaacgg
     2641 cccggattcc gccttttctt cttacttcga tatagattgg catccgcctt cgcgaaatct
     2701 ggacaacaag attctcttgc ctatacttgg gcggccattt ggggaagcgc tggacagcag
     2761 cgaactgaaa cttaattttc gcgacggtca attctccatc gaatattt
//
LOCUS       DATCBB010001026         1173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_291735, whole genome shotgun sequence.
ACCESSION   DATCBB010001026 DATCBB010000000
VERSION     DATCBB010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291735"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VII37_11200"
     CDS             complement(<1..642)
                     /locus_tag="VII37_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070354.1"
                     /translation="MRTTLKIVLPLIISVAVVSLLFAAYQVRTDRRILRNDLSRRAEI
                     LGESLQDNVEPMLDKAGDKNLQRLVDRFGQREHLKGVAVYGADGKVLAITPGLPEVFR
                     SEPASAVHAAQKDAGFGEFIKVEHLPMHVYALPLHSNGKAAGALALFHDTTFIETQVS
                     HTLRDALLNALIQTLLITGLAMILVRWTFTNPLTRTAKWLRTLRTGQSHTPPTL"
     gene            899..>1173
                     /locus_tag="VII37_11205"
     CDS             899..>1173
                     /locus_tag="VII37_11205"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5070355.1"
                     /translation="MPKMKVAQISAPGAEFELVEREIPNPPAGHVRIKVQACGICHSD
                     ALTKEGHWPGITYPRVPGHEIAGIIDELGAGVSVWKKGQRVGVGWHGG"
BASE COUNT          255 a          377 c          308 g          233 t
ORIGIN      
        1 aagcgttgga ggcgtatgcg attgcccggt gcgcagtgtg cgcagccact tggcggtgcg
       61 cgtcaatgga ttcgtaaacg tccagcgcac caggatcatc gccagcccgg tgatcaacag
      121 cgtttgaatc aacgcattca acagcgcatc gcgcagcgtg tgcgaaacct gcgtctcaat
      181 aaacgtggtg tcgtgaaaca gcgccagcgc gccagccgct ttcccgttgc tatgcaaggg
      241 cagcgcgtaa acatgcatcg gcaaatgctc aactttgatg aactctccga agccggcgtc
      301 tttttgcgcc gcgtgtaccg cgctcgctgg ctccgaccgg aacacttcag gcagcccagg
      361 cgtaatcgcc agcacctttc catcggcgcc gtacaccgct acgcctttta ggtgttcccg
      421 ttgtccaaag cgatccacca gccgttgcag gttcttgtcg cccgccttgt ccagcatcgg
      481 ctcgacgtta tcctgcaggc tttcgcccaa tatttccgcg cggcgggata gatcgttgcg
      541 cagaatgcgc cggtccgtgc gcacctggta agcggcaaaa agcagggaaa ccacggctac
      601 gctgatgatt aacggcagga caatcttcag cgtcgtgcgc aaatcagctc cttaaatcgc
      661 ctggaaaaac ctgcgcccag ctgtttggcc cccgaataat gggccgcggc agcactccag
      721 cgctcgggca agtatcttaa tccaaagcca gtaaccttgc actgtagccc gaaacgcagt
      781 cgcgcttctc acacaaatgt tagtttacgg cgctcatttt cctctaacct gcctctgagc
      841 atccgcatcc aaccacccgt cttcgagaaa ttcatttttc aattcaagga gtcccctcat
      901 gcccaaaatg aaagtcgcgc aaatcagcgc tcctggcgcc gaattcgaac tggtcgaacg
      961 ggaaatcccc aatccccccg caggccacgt ccgaatcaaa gtgcaagcct gcggcatctg
     1021 ccacagcgac gccctcacca aagaaggcca ttggcccggc attacctacc cgcgcgtccc
     1081 tggccatgaa atcgccggca tcatcgacga gttgggcgca ggcgtctcag tctggaaaaa
     1141 aggccagcgc gttggcgtcg gctggcatgg cgg
//
LOCUS       DATCBB010001027         1206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_452961, whole genome shotgun sequence.
ACCESSION   DATCBB010001027 DATCBB010000000
VERSION     DATCBB010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452961"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..>1206)
                     /locus_tag="VII37_11210"
     CDS             complement(188..>1206)
                     /locus_tag="VII37_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY5070356.1"
                     /translation="PQDGGRFITFPLVFTKNPETGKRNVGMYRMQIYDERTTGMHWQT
                     QKHGAEHFRRARAANPEGKIAVAVAIGADPATALAGMLPIPPDLDEMMFSGFLRREPV
                     EMVQCETCDLEVPANAEIILEGYVNLNEMRVEGPFGDHTGFYSLEGEYPVFHVECITH
                     RKDPIYLTTVVGPPPQEDYFMGYAVERVFLPVMKMQYPEIADVAMPAEGIFHNLMIVA
                     IRKSYPGHARKIMNAIWSLGQAMFTKVIVVVDHDVDVHNYSEVAWKALCAINPERDVQ
                     FNLGPADTLDHASRIQDFGSKMGIDATRKWASEGFARPWPDEILMDAGTKSRIDSLWK
                     SFGL"
BASE COUNT          293 a          404 c          241 g          268 t
ORIGIN      
        1 agatcgttca gagccctgga ggagtccgca cagcgcccca atcgggcact gcctaaactt
       61 taaattatac gactcgcggt caacacgagc aaacgtggtc cgactgcgac caattccctg
      121 accttttact ttttccttaa agcgtgggta tttcttagtg caggcgaaat ttaaagagca
      181 gaatcgttca caagccgaaa ctcttccaca acgaatcaat acgcgacttc gttcccgcgt
      241 ccatcaaaat ctcatcaggc cacggccgcg caaatccttc cgaggcccac ttccgcgtag
      301 catcaatacc catcttcgat ccaaaatcct gaatccgcga agcatgatcc aacgtatccg
      361 ccggtcccaa attaaattgc acatcgcgtt ccgggttaat cgcgcacaag gctttccagg
      421 ccacttcgct gtagttgtgc acatccacgt catgatccac caccacaatc accttggtga
      481 acatcgcttg tcccagtgac caaatcgcat tcatgatttt ccgcgcatgc cccggataac
      541 tctttcgaat cgccacaatc atcaaattgt gaaaaattcc ctccgccggc atagccacat
      601 ccgcaatctc cggatactgc atcttcatca ccggcaaaaa aacccgttcc accgcgtaac
      661 ccataaaata atcttcctgg ggcggcggcc ccaccaccgt cgtcaaataa atcggatcct
      721 tccggtgcgt gatgcactcc acgtgaaata ccgggtactc cccttccagc gaataaaatc
      781 ccgtgtgatc gccaaaaggt ccttcgaccc gcatctcatt caaatttacg tagccttcca
      841 aaataatttc cgcgttagca ggaacttcca ggtcgcaagt ctcgcactgc accatttcca
      901 ccggctcccg ccgcaaaaat cccgaaaaca tcatttcatc caggtccggc ggaatcggca
      961 gcatccccgc cagcgccgtc gctggatccg ccccaatcgc caccgccaca gcaatcttcc
     1021 cttctggatt cgcggcccgc gcccgccgaa aatgttccgc gccatgtttt tgcgtctgcc
     1081 aatgcatccc ggtggtgcgc tcgtcgtaaa tctgcatgcg gtacatcccc acgttgcgct
     1141 taccggtctc cggatttttc gtaaacacca gcggaaaagt aatgaaccgt ccgccatcct
     1201 gcggcc
//
LOCUS       DATCBB010001028         1565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_291806, whole genome shotgun sequence.
ACCESSION   DATCBB010001028 DATCBB010000000
VERSION     DATCBB010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291806"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VII37_11215"
     CDS             <1..328
                     /locus_tag="VII37_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070357.1"
                     /translation="VNYPVVGRDAFRTATGVHAAAIIKALRKNDPELVNAVYSGVPAH
                     LFGLEQVIEIGPMSGKSNVLYWLEKRGMGGDEALVNRIYDAAKKSSRVLTDAEILALC
                     QKSAGN"
     gene            352..>1565
                     /locus_tag="VII37_11220"
     CDS             352..>1565
                     /locus_tag="VII37_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5070358.1"
                     /translation="MKHFLVWVILLSMPLRSSLAAQPPRQAGASEITKQPQTQEALDW
                     FEPHQSFINEVQIKLAEIPAPSFQEEQRAAAVAELLGASGLAVHRDKIGNVIGELRGA
                     NEKEIVILAAHLDTVFPAGTDVKVRHEGERLSGPGISDNGTGLAALVAIARAMQAAKI
                     KPRRTILFAADVGEEGEGNLRGMRALVDAYRANLKAAIVLDGSGTDHVTTKALASQRL
                     EVAITGPGGHSWSDFGMVNPINALVRGAVRFINTKVPTAPRTTFNLGQIEGGTSVNSI
                     PHEAKLKVDIRSESEEELGRLEVALRECAAAGVRDEMESSRDRSKGKLEWKVDLLGSR
                     PGGELAAGSPVLAALRAADEFVGNHSRLERSSTDANIPLSVGLDAIAIGAGGNGGGAH
                     SLQEWYDPAGRE"
BASE COUNT          327 a          349 c          563 g          326 t
ORIGIN      
        1 ggttaattat ccggtggtgg gtcgcgacgc tttccggacg gcgacgggag tacatgcggc
       61 agcgattatt aaagcgctgc gaaaaaatga ccccgaactg gtcaacgcgg tttacagcgg
      121 cgtgcctgcg caccttttcg gactggagca ggtgattgag attgggccca tgagcggaaa
      181 atcaaatgtg ctttactggc tggaaaaacg cgggatgggc ggggatgaag cgctcgtaaa
      241 tcgcatttac gatgcggcga aaaaatcttc gcgagtgctg acggatgcgg agatcctggc
      301 gctttgccag aaatcggcag ggaattgaag cggcgcaata aaaaccgcac gatgaaacac
      361 tttttagttt gggttattct gctaagcatg ccgctgcgat cttcacttgc cgctcaaccg
      421 cctcgccaag ctggtgcgtc cgagattacg aaacagccgc agacgcagga ggcgctggat
      481 tggttcgagc cgcaccagtc tttcatcaat gaggtgcaaa taaaacttgc ggaaattccc
      541 gcgccttcgt ttcaggaaga acaacgcgcg gcggctgtgg cggaactgct aggcgcctcg
      601 gggctcgcgg tgcatcgcga caagattggg aatgttattg gggaattgcg cggtgctaat
      661 gagaaagaga tcgtaatttt ggcggcgcat ctggacacgg tgtttcctgc gggcacagac
      721 gtgaaggttc gtcatgaagg ggaacggctg agcgggccgg ggatttcgga taatggcacc
      781 gggttggctg cgctggtggc gattgcgcga gcgatgcagg ccgcaaaaat taagccgcgg
      841 cgaacgattt tgtttgccgc cgacgtgggc gaagagggcg aaggaaattt gcgggggatg
      901 cgcgcgttgg tggatgcgta ccgcgcgaat ctaaaggcgg cgattgttct ggacgggtca
      961 ggtacagatc atgtgaccac gaaggcgttg gcatcgcaac ggctggaagt ggccatcact
     1021 gggccgggcg ggcacagctg gtcggatttt gggatggtga atcctatcaa tgcgctggtg
     1081 cgcggcgcgg tgcgatttat taataccaaa gtgcctacgg cgccgcggac gacgtttaat
     1141 ttggggcaga ttgaaggggg cacgtcggta aattccattc cacatgaggc aaagctgaag
     1201 gtggatatac gttcggagag cgaagaggag ttggggcggc tggaggttgc gctccgggaa
     1261 tgcgcggctg cgggagtgcg ggatgagatg gagtcgtcgc gcgaccgttc gaaagggaag
     1321 ctggaatgga aggtggatct gctgggcagc cggcctggtg gtgaactggc tgcaggttcg
     1381 cccgtgctgg cggctttgcg tgcggcggat gaatttgttg ggaaccattc gcggctggag
     1441 cggtcgtcca cggatgcgaa cattccgctt tccgtgggtc ttgatgcgat tgcgattggg
     1501 gcgggcggaa atggcggtgg ggcgcattct ttgcaggagt ggtatgaccc tgccgggcgc
     1561 gagat
//
LOCUS       DATCBB010001029         1341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_356348, whole genome shotgun sequence.
ACCESSION   DATCBB010001029 DATCBB010000000
VERSION     DATCBB010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356348"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VII37_11225"
     CDS             <1..1025
                     /locus_tag="VII37_11225"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070359.1"
                     /translation="HDPPRKSELKRLKGFVAREITRVQDRIKSARVGTVIATSGTAAA
                     LAGAANNLTRPRTSRTATYATREMIRRVVKQITHQTLEQRTKVPGIGPRRAEIICAGA
                     VVYSELLERFHLPGFRYSALGLRDGILAQMAAEYDRSTRSGRAIESERWDSIKRAVEH
                     YRVDLNHALLVRESALLLFSSLKSVHQLPPEYREWLSAAAMLYEVGDYVNRNGHHRHT
                     YYIIANSEILGYTPQQRQIIGSIARYLGKSRPTPGDGPMNGLTAEEQENITKASILLR
                     MARALHMGRIHSVERFSVSSRSGKVAMKLVARGKASVDLEVWAIEKDRLYFREVFGRE
                     LSVAAV"
     gene            complement(977..>1341)
                     /locus_tag="VII37_11230"
     CDS             complement(977..>1341)
                     /locus_tag="VII37_11230"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEY5070360.1"
                     /translation="KRATQTAWLVGNEMGHEGKLVIETALRPQASFSDFQKMLEKYAR
                     QDSVLVVGHNPNLREFLGRIISDSGCEAVVDLKKGAVAKVEMRRNSGSLSWCFTPRIL
                     RTLHTAATESSRPKTSRK"
BASE COUNT          293 a          371 c          389 g          288 t
ORIGIN      
        1 aacacgatcc tccgcgcaag agcgaattga aacggcttaa gggatttgtg gcgcgagaaa
       61 ttacgcgcgt acaggatcgg attaaatccg ctcgcgtagg aactgttatc gctacctccg
      121 gcacggcggc agctttggcg ggtgcggcca acaacctgac gcggccacgg acgagccgca
      181 cggcgactta cgctacgcgt gagatgatcc gccgcgtcgt aaagcaaatt actcatcaga
      241 cgctggagca gcgaacgaaa gtgcctggga ttggcccgcg tcgcgccgag atcatttgcg
      301 caggagcggt ggtctactcg gaattgctgg aacgctttca tctccctggc tttcgctact
      361 cggcgctcgg gctgcgcgac ggcatccttg cgcagatggc ggccgaatac gatcgcagca
      421 cccgatcagg acgggcgatt gaatcggaac ggtgggactc gatcaagcgc gctgtagaac
      481 attaccgggt cgacttgaat catgcattgc tggtgcgcga gtcggcgttg cttttatttt
      541 catcgctgaa atcggtacac caacttccac cggagtatcg ggaatggctt tcggctgccg
      601 ccatgcttta cgaggtgggc gattacgtca accgaaacgg ccatcatcgc cacacttact
      661 acattattgc caactcggaa attctgggat acacgccaca gcaacggcaa atcatcggct
      721 cgatcgcgcg ttatctgggg aaatcgcgac ccactccggg cgatggcccg atgaacggcc
      781 tgactgccga agagcaggag aatataacga aggcttccat actgctccgc atggcgcggg
      841 ctctccatat gggcagaatc cattcggtgg aaagattcag cgtttcgtcg cgcagcggta
      901 aagtggcgat gaaactggtc gcgcggggaa aggctagcgt ggacctggaa gtgtgggcga
      961 ttgagaagga tcgactttat ttccgcgagg tcttcgggcg cgagctctcc gtggccgcgg
     1021 tatgaagcgt gcgtaaaata cgtggcgtga aacaccagct cagggaaccg gaattgcgcc
     1081 gcatttcgac ttttgcaaca gcgccttttt tgagatcgac cacggcttcg catccgctgt
     1141 cgctgatgat gcgtccgaga aattcgcgca ggttcggatt atgccctact accagcacgg
     1201 aatcctggcg cgcatacttt tccaacatct tttgaaagtc cgagaaactc gcttgcgggc
     1261 gcaatgcagt ctcaattacc agcttgccct catgtcccat ttcgtttccc actaaccagg
     1321 cagtctgagt cgctcgtttt a
//
LOCUS       DATCBB010001030         1160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_356365, whole genome shotgun sequence.
ACCESSION   DATCBB010001030 DATCBB010000000
VERSION     DATCBB010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356365"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VII37_11235"
     CDS             <1..371
                     /locus_tag="VII37_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5070361.1"
                     /translation="VSRWSSAGLAERNPAAIARMDSMIRKTSANGYCGCAAALARLDY
                     GSRLQEINAPALVICGSEDRGAPPANSRQMQVMIKDARFLEIKQAGHISNIEQPIIFN
                     AAVRNFLDDVESRQALSPWD"
BASE COUNT          256 a          326 c          329 g          249 t
ORIGIN      
        1 cggtatcccg ctggtcgagc gccggattgg ccgagcgaaa tccagccgcg attgcacgga
       61 tggattccat gatccgcaaa acatccgcca acggctattg cggctgcgcg gccgcattag
      121 cgcggctcga ctacggctcc aggctgcagg aaatcaatgc tccggcgctt gtgatttgcg
      181 gcagcgaaga ccgtggtgcg ccgccggcaa acagccgcca gatgcaggtg atgatcaaag
      241 acgcgcgttt ccttgagatc aaacaggccg gccatatatc caacatcgag cagcccataa
      301 tcttcaacgc tgcggtccgc aattttctgg acgacgttga gtccaggcaa gccctttcgc
      361 cttgggactg agcggactgc gtggtggcgg acgcagtcgc agtcgaacct gtctccccca
      421 ccaaattccc tgctaacagg gaaaagaaca gggaattttt caattttggt ctagcttgcg
      481 gcacagatgc tgccattcgc ccagtgattt agggagctta gagataaaat cgcgatattc
      541 gctacgtgcg aagaagccca gcgcactgcc gatgcccacg tgcgtgacgg ctatccgaac
      601 accgagataa tcgatgatgg tttttcgtgg ctcccagagg cagacattga ctggaggacg
      661 tgtccctatc gggttgccgc aagggctagg ctggcagtcc atgcgcgtgc gtgattatca
      721 cgtcagcagg tgcgcaactc gccgctaaaa ccggactgcg tatctgttgc ccggtagcaa
      781 ggtgacgttt tagcgggctg accgtcatgg cacgataaaa cgctctctcg cgccgttcgc
      841 tgttaccttg acccctgcga gtaagtggtc ggaagcggaa aatatcgccg ccgctatacg
      901 ctcaatctcg catcaactgc attgcaaacg tcaaatggtt gggaaggtcg tcaatgttcc
      961 cgcgcatgag ccaccgatta atgcgtcaaa cttgaaattt gcattaatct ttcgagctcc
     1021 cgacggccat caatgcgcgc ctcttgctcg cctgagaacg attgtatgga agattggaca
     1081 ggcgttccat cccgtcggat gtcccaacga tagcgccccg tttggatcat gcagggtcgg
     1141 attataaggt cgtacggcgg
//
LOCUS       DATCBB010001031         1143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_388564, whole genome shotgun sequence.
ACCESSION   DATCBB010001031 DATCBB010000000
VERSION     DATCBB010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388564"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..>1143)
                     /locus_tag="VII37_11240"
     CDS             complement(384..>1143)
                     /locus_tag="VII37_11240"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5070362.1"
                     /translation="NAQTSGRAGAITPTNEAGRKIYVNGEGGGGHPVSPSAQVASKSS
                     LVYWSSTEHRFKPVPTSGAVMRAAHSAASEVNTYLDKRANIHQTLNRAFTQQDIDAAI
                     DDAAARHNVDPSLVRSVVKVESNFNPNAVSRKGAMGLMQLMPSTARSLNVSNPFDPAQ
                     NVDAGVRHLRRLLDNYGGDVRLSLAAYNAGSGAVARSAGVPHFRETQNYVRRITNLYS
                     GGQEAGSYVIGGAVRAPVHVQRDARGVLYISNTE"
BASE COUNT          248 a          340 c          310 g          245 t
ORIGIN      
        1 gtaggaatcg ataacacgct gatattcgcg gcgggtacgt tgcgccagcg gccggccgtt
       61 tagagcttca cgcatgcgct ccgcgtgggc gaattgctga cgcgcccaag cgtcattgtg
      121 atgcacgcgc gcggaggccg aagccaaggg caggagaaac agcaatagcg ctgcgccgag
      181 gcgaaaagcg cgagtgcctc gaattagggt cgtagaagcc tgagtcttca cttgcctgtt
      241 acctacttac cggctaaata cacatcccac accttgggcc tgatttcagc ctctctcttg
      301 cagtgcgaaa tggattactt gaacccttaa aatgacaacc cagcttcaat cgcgaagttg
      361 ctggacttta acccaaaaaa aatctactcc gtgttgctga tgtacagcac tcccctggca
      421 tctcgctgca catgaaccgg ggcgcgtacc gcgccgccga taacgtagga gcccgcctct
      481 tggccgccgc tgtacagatt ggtaatacgg cgaacataat tctgcgtttc gcgaaaatgc
      541 ggaacgcccg cgctccgcgc gaccgcgccc gaaccagcgt tataagccgc cagactgagc
      601 cggacatcgc caccatagtt atccaacaat ctccttagat gccgaacccc ggcatcgacg
      661 ttttgcgccg gatcgaaagg attggaaaca ttgagtgagc gggcggtcga gggcattaac
      721 tgcatcagtc ccatggcccc tttgcgcgac accgcattgg gattgaagtt cgattccacc
      781 ttgaccaccg accgcaccag gctcggatcc acattgtgcc gggccgctgc atcgtcaatc
      841 gcggcgtcaa tatcttgttg agtaaaagca cggttaagcg tctggtggat attggctcgc
      901 ttgtcgaggt atgtgtttac ctccgatgcg gctgagtgcg ccgctcgcat cacagctccg
      961 ctggtcggaa ccggcttgaa tcgatgctcg gtcgaactcc aataaaccaa tgaggatttg
     1021 gacgcaacct gggcagaagg agacactggg tgcccgccgc ctccttctcc atttacgtaa
     1081 atctttcttc ctgcctcgtt agtaggagta atggccccgg ctcgcccgct ggtctgcgca
     1141 ttc
//
LOCUS       DATCBB010001032         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_356557, whole genome shotgun sequence.
ACCESSION   DATCBB010001032 DATCBB010000000
VERSION     DATCBB010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356557"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VII37_11245"
     CDS             complement(<1..334)
                     /locus_tag="VII37_11245"
                     /inference="COORDINATES: protein motif:HMM:NF016203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY5070363.1"
                     /translation="MAQHSFFGYRRENGRVGIRNHVVILPLDDLSNASAEAVANNIKG
                     TLALPHAYGRLQFGEDLELHFRTLIGTGSNPNVAACVVIGIEPSWTGRVVEGIANTGK
                     PVVGFAIEG"
     gene            complement(337..621)
                     /locus_tag="VII37_11250"
     CDS             complement(337..621)
                     /locus_tag="VII37_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY5070364.1"
                     /translation="MATDFIVHDKADTVGVVVVEDVQPGKEVTGWIMETDETIRFKSL
                     DAVPLGHKIALRDLKVGDTVLKYGHDVGRMVANVGQGRHVHVHNLKTKRW"
     gene            complement(657..1388)
                     /locus_tag="VII37_11255"
     CDS             complement(657..1388)
                     /locus_tag="VII37_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5070365.1"
                     /translation="MMQQSNHLSSTAPLSFIERSAAPLRRQVLDELRQSIIAGRLAPG
                     SRLIERELIAMMGVSRTVIREALRQMESEGLVANIPNKGPVVRELTLAEAKDLYSIRA
                     VLEGLAARLFTENASAAQKKLLERALKATAQSYEHGDPAAILESKNRFYEVLFQGAGS
                     ETLSSMIGTLHVRIWRWRALGLGHPRRSEKRWKESIRGLRAMIAAISKGDASLAETLL
                     RDEVTKAAAEVMRLLINKTPLTDGH"
     gene            1472..>2426
                     /locus_tag="VII37_11260"
     CDS             1472..>2426
                     /locus_tag="VII37_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5070366.1"
                     /translation="MSDKPVKAGIVGLGRWAKVLTKASKKSKLIEIVAGYSRSEEKRT
                     AFQQELGVPAAASMQAILSDPEIEGVILTVPNEQHLPMAREVAKSGKHVYTEKPIAST
                     LEDGLEIAALQQKYGITVTVGHSARLMAGIRKIGEAIAAGELGRVAFIEANFSNERAL
                     ELTPKTWRWYKDKAPGGPLSQLAIHQFDVMQYLGGEVVSASSIASKLSPVGAEVDDQS
                     MTLLKFADGKIGYVGSCWTSPGVFAVRVFGSKGLMHYEIDFGTWDTPDKLHESSTLYI
                     QRGKNGYGSREEMKVPPSDMFRAELELFAESCRTGKPNELTA"
BASE COUNT          552 a          616 c          732 g          526 t
ORIGIN      
        1 ggccttcgat agcgaagccg accacaggtt tgccggtgtt ggcgattcct tcgaccactc
       61 gcccggtcca gctcggctcg atgccgatca cgacgcaagc cgcgacgttg ggattgctgc
      121 cggtgccaat cagcgttcgg aaatgcagtt ccaggtcctc gccaaactgc aagcgcccgt
      181 aggcgtgagg aagcgccagc gtccctttaa tgttgttggc gacggcttcg gcactggcat
      241 tggacaagtc gtcgagtggg agaataacca cgtgattacg tatgcccacg cgtccgtttt
      301 cacgtcggta gccaaaaaat gagtgctgcg ccacgtttac cacctcttgg tttttagatt
      361 gtgtacgtga acatggcggc cttggccgac attagcaacc atgcggccta catcatgtcc
      421 atacttcaga acggtgtcgc caactttgag gtcacggagg gcaatcttgt gaccgagcgg
      481 cacggcatcg agcgatttaa aacgaatggt ctcgtcggtc tccatgatcc aaccggtaac
      541 ttccttgcct ggctggacgt cttcgacaac cacgacgcca acagtgtcgg ccttgtcgtg
      601 cacgatgaaa tcagtggcca tgaaaaattt cctccgaaga cttgcaggca gccaatctaa
      661 tgtccgtccg tgagcggcgt cttgttaatc agcaagcgca ttacttcggc ggccgcttta
      721 gttacttcgt cgcgcagaag agtctccgcc agcgaggcat cgcctttgga gatggccgcg
      781 atcattgcac gcaagccgcg aatactttcc ttccaacgct tttcagagcg ccgcggatgt
      841 cccaatccaa gcgcgcgcca acgccagatg cgcacgtgta gcgttcctat catagacgag
      901 agcgtctcgc tgcccgcgcc ttgaaacaag acttcgtaaa agcgattttt ggattcaaga
      961 atggcagccg gatcgccatg ttcgtaggat tgtgcggtgg ctttcaaggc tcgctcgaga
     1021 agtttcttct gcgccgcgct ggcattttcc gtgaacaggc gcgcggccaa tccttccagg
     1081 accgcacgaa tggaataaag atctttcgct tccgcaaggg tcaattcgcg aaccaccggg
     1141 cctttgttgg ggatgttagc gaccaggcct tccgattcca tttggcgaag ggcctcgcga
     1201 atgaccgtgc gcgatacgcc catcatggcg atgagctcgc gttcgatcaa acgggagccc
     1261 ggggcgaggc ggccagcgat gatggattgt cgcaattcat ccagaacctg gcggcgcagt
     1321 ggggcggcgg atctttcaat gaacgagagg ggcgcggtgg aggacaaatg atttgattgt
     1381 tgcatcatcc cgtaatacgg tactatcagg gctgcgagtt tgcaagaaat tgttgtattt
     1441 tccaggccat gaattttttt gaaagggcgc aatgagtgac aagccggtaa aagcagggat
     1501 tgtagggcta gggcggtggg ccaaagtgct caccaaggcg tcgaagaaat cgaagttaat
     1561 agaaattgtc gctggctaca gccgttcgga agagaagcgg acagcttttc agcaggagct
     1621 tggagttcca gcggccgcga gcatgcaagc gatcctcagc gatccagaaa ttgagggtgt
     1681 cattctcacc gtgcccaacg agcagcacct gccgatggcg cgggaggtgg cgaagagtgg
     1741 gaagcacgtg tacacggaga aaccaatcgc aagcacgctt gaagatggat tagaaatcgc
     1801 ggcattacag caaaagtatg ggattaccgt taccgtgggc cacagcgcgc ggctgatggc
     1861 tggaatccga aaaattggcg aagctattgc cgcgggagaa ttgggacgcg tggctttcat
     1921 tgaagcgaac ttttcaaacg aacgcgcgtt ggaattaacg cccaagacgt ggcgctggta
     1981 taaagataaa gcgccgggcg ggccgctgtc gcaattggcc atccatcaat tcgacgtgat
     2041 gcaatatctc ggcggggaag tcgtttccgc gagttccatt gcgtcgaaat tatcgccggt
     2101 gggcgcggaa gtggacgatc agtcgatgac cttattgaaa ttcgcggatg gaaaaattgg
     2161 gtacgtgggt tcgtgttgga cttcgccggg ggtatttgcg gtgcgagtgt tcgggtccaa
     2221 gggattgatg cattacgaga ttgattttgg cacctgggac acgccggata agttgcacga
     2281 aagctccacg ctgtatattc agcgaggcaa aaatgggtac ggcagccgtg aagagatgaa
     2341 agtgccgccg agcgatatgt ttcgcgcgga gctggagcta tttgccgaaa gctgccggac
     2401 cggcaagccc aatgaactta cggcgg
//
LOCUS       DATCBB010001033         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_194274, whole genome shotgun sequence.
ACCESSION   DATCBB010001033 DATCBB010000000
VERSION     DATCBB010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194274"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..910)
                     /gene="bshB1"
                     /locus_tag="VII37_11265"
     CDS             complement(194..910)
                     /gene="bshB1"
                     /locus_tag="VII37_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEY5070367.1"
                     /translation="MHLDLLAIAAHPDDVELSCGGTMLRMAQQGYKTGILDLTAGEMG
                     TRGTRETRAKEADAAGKILQVSWRGILGVPDSDVQASRANKVLLAAKIRELRPKTVIL
                     PYWEARHPDHYHASTLGYEGCFLAGLKQLPIEGETYRPFKILYSTAYANVRPTFVVDI
                     SAQYDERKRAILAYSSQFPGNVPVNKEGVHLGLDELENEMSQLARHYGQMIGVKYGEP
                     FLIKELVKVDDVVKMDVRSI"
     gene            1048..1794
                     /locus_tag="VII37_11270"
     CDS             1048..1794
                     /locus_tag="VII37_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5070368.1"
                     /translation="MPTSGSNRRKLKGKVALVTGAAKRLGRAIALHLASEGADVVVHF
                     HHSDLEAQEVVTQISSLGRRAIYLSADLAKKSDIDKLFANTTKEFGRLDILINNASNF
                     LHADFPSITEEIWDAAIDVNLKAPFFCSQAAAPLLKKNNGSIINLSDVAGFLGWTGYI
                     PHSVSKAGVSMLTRVLAKALAPEIRVNAIAPGTITMPGDPPDLAADFIKRAPLQRTGT
                     TADVTAAISFLINSPFITGEVLVLDGGRTL"
     gene            1938..>2622
                     /locus_tag="VII37_11275"
     CDS             1938..>2622
                     /locus_tag="VII37_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070369.1"
                     /translation="MDNDIFTHPNETFAQPDEAPAPAQPHGIDDVEIQEWLESLDAVL
                     ESSGAEVAAEILERLRAHATVNGIDLPFTANTPYANTIPHRLEPRYPGDQELERRIKS
                     LVRWNALAMVVRANRVEHNIGGHISTFASAATLYEVGQNHFFRGRTENFEGDTVYFQG
                     HAAPGMYARAFLEGRISAQQLENFRRELKPGGGLSSYPHPWLMPDFWEYPSVSMGLSP
                     LMAIYQARYN"
BASE COUNT          557 a          915 c          595 g          555 t
ORIGIN      
        1 ggacacctac ctggtcaccc gcctatacac cgaggccctg agcaccatgg tcatcctgcg
       61 caaatcgggc tccgaagagg aagccctgac acgcgtgcgc gccgagcgca cccccacagg
      121 acaaaatatc cctggctttt cccccgcgca ggaaaacgaa acccgttaga tttttcagaa
      181 aagctcagcg cctctagatc gagcgcacgt ccatcttcac cacgtcgtca accttcacga
      241 gttccttaat cagaaaaggc tccccgtatt tcacaccaat catttgcccg tagtgccgcg
      301 ccagttggct catctcattt tccagctcgt caagccctag atgcacacct tctttgttca
      361 ccggcacgtt cccgggaaat tgcgaggagt acgccagaat cgcacgcttg cgttcatcat
      421 attgcgcgct gatatccacc acgaaagtcg ggcggacgtt agcataagcc gtggaataca
      481 aaatcttgaa cggccggtaa gtctcgccct caatcggcag ctgtttcaat cccgctagga
      541 agcagccctc ataccccagc gtcgacgcat gataatgatc cggatggcga gcctcccaat
      601 agggaagaat cactgtcttc ggtcgcaact cccggatctt ggccgccagc agcactttgt
      661 tcgctctgct ggcctgcaca tcggagtctg gaaccccgag aatcccgcgc caagacacct
      721 gtaaaatttt gcccgccgca tccgcttctt tcgcccgcgt ctcgcgcgtt ccccgcgtcc
      781 ccatttcccc ggcagtcagg tcgagtattc cggtcttata tccctgctgt gccatgcgca
      841 gcatcgtccc cccgcaactc agctccacat cgtccggatg cgcggctatc gccaataaat
      901 caagatgcat ttcccctccg cggaattcga ttctaacagc acgcctcatc tagcatcgtc
      961 tatccttctt aaccacccgc agcagcggcg ccattgtcac cgaattgtct gccgcttctc
     1021 ctgagcgaat gtgctacttt tttagcaatg cccacctccg gttcgaatag gcgcaaactc
     1081 aaaggaaaag ttgctctggt caccggagcc gccaagcgtt taggacgcgc catcgcgcta
     1141 cacctcgcta gcgaaggcgc ggacgtagtt gtccatttcc atcattcaga cttggaagcc
     1201 caggaagtag taacgcaaat ctctagcctc ggccgccgcg caatatacct gtccgccgac
     1261 ctcgcaaaaa aatccgacat cgacaagctc ttcgcgaaca ccaccaaaga atttggccgc
     1321 ctcgacattc tgatcaacaa cgcctctaat tttcttcacg ccgacttccc atccatcacc
     1381 gaggaaatct gggacgccgc catcgatgta aatctgaaag ccccattttt ctgctcccag
     1441 gccgccgcgc ccttactgaa aaaaaacaac ggcagcatca tcaatctttc tgacgtcgcc
     1501 ggcttcctcg gctggaccgg ctacatcccc cacagcgtat ccaaagcagg ggtcagcatg
     1561 ctcactcgcg tgctcgccaa agcgctcgcc ccagaaatcc gcgtcaacgc catcgctccc
     1621 ggcaccatca ccatgcccgg tgacccgcca gatttggcag ccgacttcat caagcgcgct
     1681 ccgctccagc gcaccggcac cacggcagac gtcaccgccg ccatctcctt cctcatcaac
     1741 tcccccttca tcaccggtga agtcctggtt ctggacggcg gtcgcactct ctagccgatc
     1801 attttcttca ctcatttttc tgcccagcgc agcataattt tgcccggagg gtgtattcta
     1861 ttctccttat cgctcgtata agtctttagc gctcgcatcg cgcgcttcct attttctaaa
     1921 gaaggaccat cgcggttatg gataacgaca tcttcacgca ccccaacgaa acctttgcgc
     1981 aacccgacga ggcaccggcc cccgcgcagc cccacggcat cgacgacgta gaaattcagg
     2041 agtggcttga gtctctcgac gccgtcctcg aatccagtgg cgcggaagtg gccgccgaaa
     2101 ttctcgagcg tctgcgcgcc cacgccaccg tcaacggtat cgacttaccc ttcaccgcca
     2161 acacgcctta cgctaacacc atcccgcatc gcctcgagcc ccgctatccc ggtgaccagg
     2221 aactcgagcg ccgcatcaag agcctggtgc gctggaacgc gctggccatg gtcgtgcgcg
     2281 ccaatcgcgt cgaacacaat atcggcggcc acatttctac ctttgcctcc gctgccactc
     2341 tgtatgaagt cggccagaat catttcttcc gcggccgcac agaaaatttc gaaggcgaca
     2401 ccgtttattt ccagggccac gccgcgcccg gcatgtacgc gcgcgccttt ctggaaggcc
     2461 gcatctccgc gcagcaactc gaaaattttc gccgcgagct gaaacccggc ggcggccttt
     2521 cttcttatcc gcatccctgg ctcatgcctg atttctggga atatcccagc gtctccatgg
     2581 gcttgagccc gctgatggcc atttatcagg cgcgctacaa cc
//
LOCUS       DATCBB010001034         1034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_388982, whole genome shotgun sequence.
ACCESSION   DATCBB010001034 DATCBB010000000
VERSION     DATCBB010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388982"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VII37_11280"
     CDS             complement(<1..595)
                     /locus_tag="VII37_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5070370.1"
                     /translation="MNFERAIFLFAAGILGGILNAVAGGGSFIGFPALLFAGVPPIPA
                     NATNTVALWTGVTASGGAYRKRLDVPRRVLSPLLLSSCAGGIAGALLLLKTPPHIFMR
                     VLPWLMLGATMLFIFGKRLARGRASSVGHEATASAILVASVFELAVAIYGGYFGGGVG
                     IVNLAMFAALGMTDIHAMNALKSVLGSMINGVAVVIFI"
BASE COUNT          275 a          281 c          283 g          195 t
ORIGIN      
        1 cgatgaagat caccacagcg acgccattga tcatgctgcc cagcacgctc ttcaacgcat
       61 tcatggcgtg gatatcagtc ataccgagcg cggcaaacat cgcgaggttt acgatgccga
      121 cgccgccgcc gaaatagccg ccgtagatgg ccacagccag ttcaaacaca gacgcaacaa
      181 gaatcgcaga cgcggtcgct tcgtgaccca cgctggatgc tcgtcctctc gccaggcgct
      241 tcccgaaaat aaacagcatg gtcgcgccaa gcattagcca tggcagaact cgcatgaaaa
      301 tatgcggagg agtcttcaag agaagaagcg cgcccgcaat gccgccggcg caactgctaa
      361 gcagcagcgg cgacaatacg cgcctcggta cgtcaagccg ctttcggtaa gcgccgccgc
      421 tcgccgtcac accggtccac agcgcaactg tatttgtggc attcgcgggg atgggaggaa
      481 caccggcgaa tagcaaggca gggaaaccaa tgaaacttcc cccgccggct acggcgttca
      541 gtattcctcc tagaatgccg gcggcgaaca aaaagatggc ccgttcaaaa ttcaagaaac
      601 atatgtatca cagtggcaga ccacgaattg attttttggg ctttccagca ttttgaggtc
      661 accaccgttg caagtaacgt gcctgagcag ccattggctt gctagcacat ccagtggaga
      721 gcgaatttca atgccaggaa aaaatgggca agaagagtaa agcaaaaggg aaacgggcgc
      781 ttgcggatcc gtcacggctg aagcggcacg gtgtcggtaa acccagtgca tggcgagtaa
      841 agggatcctt cgacttggtt cttcttcgct agcgaagaag aacaggcgct caggatgaca
      901 atctttatat ggtttttatt tttattgtca tgctgaacct gagcttccgc tcgcggaagc
      961 tcagatagaa ttgatggcgg gaaaaaagac gcggacgaaa agacagccgg ctacaggccg
     1021 gtgcactttc ctac
//
LOCUS       DATCBB010001035         1321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_194422, whole genome shotgun sequence.
ACCESSION   DATCBB010001035 DATCBB010000000
VERSION     DATCBB010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194422"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VII37_11285"
     CDS             complement(<1..914)
                     /locus_tag="VII37_11285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5070371.1"
                     /translation="MPEQQKTGSWLNRTVLGVGLTSLFSDWSHETATAVLPAFLAVIG
                     AGPAWLGAIEGISDGLSSFAKLASGHFTDRLKHRKPLAVAGYAFTALATASFAFATHA
                     YHVLLGRTAAWLGRGIRSPAKKALLAADTPPGAYGRAFGLERLMDTVGAIVGPLTALW
                     LLEVTGHSYRKVFLWTLLPGLLAAVLFWLLVREKPIVAKPKQSFFGGIRALPIPFRRL
                     LFGVGIFGAGDFSHTLLILYASRMLSPLQGPARAASIAVGLYTLHNVFYAGSAYVSGW
                     LSDHVRQRKMVLATGYGLAVVTAVLLCLA"
     gene            complement(972..>1321)
                     /locus_tag="VII37_11290"
     CDS             complement(972..>1321)
                     /locus_tag="VII37_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEY5070372.1"
                     /translation="MAHEKFEGERVLMRIHVGESDKWHGKSLHEAIVEILRKEGFSGA
                     TVLRGVGGFGGSSVYHTDKLLRMSQDLPIILEVIDTAERIEKILPRLDEMVEGGLITL
                     EKVRVILYRAAKR"
BASE COUNT          335 a          385 c          341 g          260 t
ORIGIN      
        1 gccaggcaca ataaaaccgc cgtcaccaca gcaagcccat atccggtggc gagcaccatc
       61 ttccgttgac gaacatggtc gcttagccac ccactcacgt atgccgaacc ggcgtaaaac
      121 acgttgtgga gggtgtaaag accaaccgcg atgctcgccg cgcgcgccgg tccctgcagc
      181 ggcgaaagca tgcgtgacgc gtagaggatt agcaaggtat gtgagaaatc accagcgcca
      241 aaaatgccca cgccaaaaag caatctgcga aagggaattg gcaaggcacg aattccgccg
      301 aaaaatgatt gcttgggctt tgcgacaatc ggtttttccc gaaccaacag ccaaaacaaa
      361 accgccgcca acaaacccgg tagtagtgtc cacaaaaata ctttgcgata cgaatggccg
      421 gtaacctcca gcaaccataa agcggtgagc ggtccaacga tcgcgccgac cgtgtccatc
      481 agtcgttcca ggccaaaggc gcgaccataa gcgccgggcg gagtgtcagc ggccagcaac
      541 gcttttttag ccggcgaacg aatgccgcgg ccgagccatg ccgcagtgcg ccccaataac
      601 acgtgataag catgtgtagc gaatgcaaat gacgctgtag ccagcgctgt aaacgcgtaa
      661 ccggccaccg cgaggggctt gcgatgtttc aagcgatcgg tgaaatgccc ggaggccagt
      721 tttgcaaagc tggataatcc gtcggagatg ccttcaatag ctccgagcca cgcggggcca
      781 gcgccaatca ccgcgagaaa cgccggcagc accgctgtcg cggtctcatg actccagtcg
      841 ctgaaaaggc tggtcaatcc gacacccaga acggtgcggt tcagccacga tcctgttttt
      901 tgttgttcag gcattctgaa attatggcag gcaattggcg gttttcatcc gaagaaaagt
      961 gcggttatca ttcaccgctt ggcagcccgg tagagaatta cgcgtacttt ttccagcgta
     1021 atcaagcctc cctcgaccat ctcatctaga cgcggcaaaa ttttctcgat acgttcggcg
     1081 gtatcgatga cctcaagaat gatgggcaga tcctgagaca ttcgcagcaa tttatcggtg
     1141 tgatatacac tggacccgcc aaatccgccg accccacgca agactgttgc acccgagaag
     1201 ccctctttgc gtaatatttc tacaatagcc tcgtgcagcg attttccgtg ccatttgtct
     1261 gattcgccga cgtgaatgcg catcaatacg cgctcacctt caaacttttc atgcgccatg
     1321 g
//
LOCUS       DATCBB010001036         3561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_389035, whole genome shotgun sequence.
ACCESSION   DATCBB010001036 DATCBB010000000
VERSION     DATCBB010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389035"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..505)
                     /locus_tag="VII37_11295"
     CDS             complement(80..505)
                     /locus_tag="VII37_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY5070373.1"
                     /translation="MTATSREAVNPKTGLTLEQMKDFIRDHFEQFVNQKNLNIADVNF
                     AVEFIDHGADVPPGTAPGPAGAKQYVGGAYKRFPDIHVQIEDLIAEDDRVVVRNHWTG
                     TDATTNQRIEFRGIVIWRIANRKIVERWAYLEKPHPADA"
     gene            complement(502..1374)
                     /locus_tag="VII37_11300"
     CDS             complement(502..1374)
                     /locus_tag="VII37_11300"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5070374.1"
                     /translation="MILITGASGNAGGAVLREVLKIGSGARAMYRSKEDSAKVPQGAT
                     AVIADFAAQQSLSRALEGIDTVYLVCSPVRELVELESNMIDASHKAGVKHIVLNSALG
                     AGDFAKSFPSWHRKVEDKLKGSGMDFTILRPNGFMQNLISYFAPSIRAEGAFYQSTGS
                     AKVSHIDVRDIAVAAAKILSSPAHHSGKIYELNGPEALSYAEIAEEISKATGRKVQYV
                     DIPANAQRKALLDMGMPDFLVTALLELQEYYASGKASKVDGTLESLIHRAPTTMDEFL
                     KEFAEQFRQQAAKA"
     gene            1625..2911
                     /gene="ispG"
                     /locus_tag="VII37_11305"
                     /gene_synonym="gcpE"
     CDS             1625..2911
                     /gene="ispG"
                     /locus_tag="VII37_11305"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY5070375.1"
                     /translation="MFTQIEGFETNVSKHPRRKTYAVKVGHVRVGGGAPVVVQSMTNT
                     DTADVESTFRQTVELAEAGSEIVRWTVNVPEAAAAVPLIKKRLLEAGCNVPIIGDFHY
                     NGHVLLTKYPECAAALDKYRINPGNVGAGQRRDEQFSTICKVARDHGKPVRIGVNGGS
                     LNQELVMQKMQENTDRSLGKESEQIINECMVLSALQSTELALESGLRNDQIIISCKTS
                     RPRDLITVYRDLSAKTEQPLHLGLTEAGMGTKGLVWSAASLGVLLYDGIGDTIRVSLT
                     PRPGGDRREEVYAACELLQSLGLRSFSPSVTACPGCGRTTSTTFQELAESVQDYIRNM
                     MPIWKTQYDGVEELTLAVMGCIVNGPGESKAANIGISLPGTGEAPRCPVYIDGKKDVT
                     LEGTYAELAVAFQALVDNYIQKHYPRKAVEAAPAGH"
     gene            2920..3222
                     /gene="gatC"
                     /locus_tag="VII37_11310"
     CDS             2920..3222
                     /gene="gatC"
                     /locus_tag="VII37_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY5070376.1"
                     /translation="MKITREDVLRVAELAYLDLSEDELEKYRRQIDEILDYIGKLNEL
                     DTSGVEPMAQVLADDQAADATMREDLVVPSAVAEDVLRQAPDPEPPYFRVPKVIER"
     gene            3324..>3561
                     /locus_tag="VII37_11315"
     CDS             3324..>3561
                     /locus_tag="VII37_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY5070377.1"
                     /translation="MTTTWTIDGVRTALTDKKISARELTSEFYKRIEARNPELNAFLA
                     LSPERARKQADKIDALVASGKPLPALAGVPIAVKD"
BASE COUNT          841 a          918 c         1005 g          797 t
ORIGIN      
        1 gctggggctg cgcggaactt tataaattcc acaggcgaga aatcacacgc aaattttatt
       61 ccggttcgat tggtaaaacc taagcgtccg cgggatgcgg tttttcaagg tacgcccacc
      121 gctccacaat ttttcggttt gctatccgcc aaatgacaat cccgcgaaat tcaatgcgct
      181 ggttggtagt ggcatccgtg ccggtccagt gattccggac caccacacgg tcgtcttcag
      241 cgatcaaatc ttcaatctga acatgaatat cgggaaaacg tttgtaggct ccgcccacgt
      301 attgctttgc tcccgcaggg cctggagcag ttccgggcgg cacatccgcg ccatgatcga
      361 taaattccac ggcaaaattc acatcggcaa tattcagatt cttctgattc acaaactgct
      421 caaagtggtc gcgaataaaa tctttcattt gctcgagggt cagtccggtt tttggattta
      481 cggcctcccg gctcgttgcg gtcatgcttt ggcagcctgt tgacgaaatt gctcagcaaa
      541 ttctttcagg aattcatcca tggttgttgg cgcgcgatgg ataagagatt cgagcgtgcc
      601 atcgactttg gatgctttgc cgctcgcgta atactcctgc agttcaagca atgcagtgac
      661 gaggaaatcg ggcatgccca tatccaggag tgctttccgc tgagcgttcg caggaatatc
      721 cacgtactgc actttgcggc cggtggcctt ggaaatttct tctgcaattt cagcgtagga
      781 cagcgcctcc gggccattta attcatatat tttcccgctg tggtgcgcag gagagctaag
      841 tattttggcg gcagctacag caatgtcgcg cacgtcgatg tggctgactt tcgcgcttcc
      901 ggtagattga taaaatgctc cttcggcgcg aatagaagga gcgaaataag aaatgagatt
      961 ctgcataaaa ccgttagggc gcagaatcgt aaagtccatt cctgacccct tgagtttatc
     1021 ttccacttta cggtgccaac tcgggaatga ctttgcgaag tctccggctc cgagcgcgga
     1081 attcaacaca atatgtttga cgccggcttt atgggacgca tcgatcatgt tgctttccag
     1141 ttcgaccaat tcgcgcaccg gagaacaaac gagatagacg gtgtcaatgc cctccagagc
     1201 tcgactaagt gattgctggg cggcaaagtc ggcgatcacg gcggtggcgc cttgtggaac
     1261 tttggcgctg tcttccttcg agcgatacat cgctcgcgcg ccgctgccaa tctttagtac
     1321 ctcacggagg actgcgcctc cagcgttacc tgatgctcca gtgattagaa tcatggattg
     1381 tcgccttagt tgacacgcgc gaaggctaca agaccgcaaa agctcagatg cactcgccct
     1441 taggacgtga ctacgacggg atggtataac aggaatttag acgccgagac cacccaattg
     1501 gctaggttgc tgaagcatct ttcggatctt cgcctccggc accaccttta cttcacgcgt
     1561 ttaaccgcat aaacagaacc gcgggatttc cagccagggc tgaatcacgt tagaatcgta
     1621 gagaatgttc acgcagatag aaggctttga gaccaacgta agcaagcatc cccggcgcaa
     1681 aacgtatgcg gtgaaggtcg gccatgtacg cgtgggcggc ggcgcacctg tagtggtgca
     1741 gtccatgacc aacacggaca cggcggacgt ggaatccaca tttcgccaaa ccgtggagct
     1801 ggccgaggcg ggttcggaga ttgtgcgttg gaccgtcaac gtgccggagg cagcggcggc
     1861 ggtgccgctc atcaagaaac gcttgctgga agccggctgt aatgtgccga tcatcggcga
     1921 ttttcactac aacggccacg tgttgctgac gaaataccct gaatgcgcgg cggcgctcga
     1981 caaatatcgt attaatcccg gcaatgtggg cgcgggacag cggcgggatg aacaattctc
     2041 taccatctgc aaagtcgcgc gcgaccatgg caagccggtg cgcattggcg tgaacggcgg
     2101 ctcgttgaat caagaactgg tcatgcagaa aatgcaggag aataccgacc ggagtcttgg
     2161 aaaagaatcc gagcaaatca ttaatgaatg catggtgctt tccgcgttgc aatccaccga
     2221 gttggcgctg gaatcaggat tacggaacga tcagatcatc atttcctgca aaacttcgcg
     2281 gccgcgggac ctcatcaccg tttatcgcga cttgagcgcg aagacagaac aaccgttgca
     2341 tctgggtttg accgaagcgg gcatgggcac caagggcctg gtgtggtccg cggcttcgct
     2401 gggtgtactg ctgtatgacg ggattggcga tacgattcgt gtgtcgctca cgccgcgtcc
     2461 aggcggcgac cgtcgcgaag aagtgtacgc agcttgcgaa ctgttgcaat cgctgggact
     2521 gcgttcgttt tctcccagtg ttacggcgtg ccctggatgc gggcggacta ccagcactac
     2581 gttccaggaa ttggcggaaa gcgttcaaga ttacattcgg aatatgatgc ccatctggaa
     2641 gacgcaatac gacggcgtag aagaattgac gctggctgtg atgggctgca tcgtgaacgg
     2701 tccgggtgaa tcgaaagctg cgaacatcgg aattagcttg ccaggaactg gcgaagcgcc
     2761 gcgctgcccg gtgtacatcg atgggaaaaa agatgtgaca ctcgaaggca cgtacgcgga
     2821 actggcagta gcttttcagg ctctggtgga caactacatt cagaaacatt acccgcgcaa
     2881 agcagtggaa gcggctcccg cggggcactg aggaatccag tgaaaattac ccgcgaagac
     2941 gtgctgcgtg tggcggaact tgcctacctg gatttaagcg aagacgaact ggaaaaatac
     3001 cggcgacaaa tagacgagat tcttgattac atcggcaaat taaacgagtt ggatacctcg
     3061 ggcgtggagc ccatggccca ggtgttggcc gacgaccaag ccgcagatgc gaccatgcgc
     3121 gaagatttag tggtgcccag cgcggtggcg gaagatgttc ttcgccaggc gcccgatccg
     3181 gaaccaccct attttcgtgt gcccaaagtg attgaacgct aggaacgcaa agccgccagt
     3241 ccatttagct agactcggcc agatttaact agtggttctg acttcattcg accagaaata
     3301 tcgcgaagga agaggctgta actttgacga cgacgtggac aatcgatgga gttcgaacgg
     3361 cgctgaccga caaaaagatt tcggctcgcg agctcacgtc cgaattctat aagcgcatcg
     3421 aggcgcgtaa tccggaatta aatgcttttt tggcgctttc tccggagcgt gcccgcaagc
     3481 aggcagacaa aatcgatgca ttggtcgcgt ccggtaaacc gttgccagct ttggctggcg
     3541 tgcccattgc tgtgaaagat g
//
LOCUS       DATCBB010001037         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_259182, whole genome shotgun sequence.
ACCESSION   DATCBB010001037 DATCBB010000000
VERSION     DATCBB010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259182"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VII37_11320"
     CDS             <1..416
                     /locus_tag="VII37_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5070378.1"
                     /translation="MKRFAEGNFQTAWGGIPSLSIALLVMYTEARARGFSLADIARWM
                     AEAPATLAGCQLHKGKIAAGYDADLAVFDESSEFLVTADRLHHRHPFSPYLDEKLKGE
                     VKATYVRGRLVFADKALQGNPQGREFPAQGRGSII"
     gene            413..>2320
                     /locus_tag="VII37_11325"
     CDS             413..>2320
                     /locus_tag="VII37_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY5070379.1"
                     /translation="MSNDRIVGKSVPRLEGRDKVTGTSRYVDDMELPDMLYGATVRSR
                     IPRGKIKSISFRPVVNWDEFVVVTAKDIPGKNCIALIGDDQPCLAEELINHPEEPILL
                     LAHPDRNLLAKAVQAVSIEYDPLPPIFTIEQSETLSEIIWGADNIFKSYLIEKGDVES
                     AFKNADYIVEAEYTTGAQEQLYIENNGMIASFDPQQGVTVWGSLQCPYYVHKALMELC
                     GLPGDRVRVVQMETGGAFGGKEEYPSMLAAHATLLAIKSGKPVKIVYDRMEDMAATTK
                     RHPSRTRHRTAVSREGKILGGEIDFTIDGGAYKTLTPVVLSRGALHAGGPYYWPSVRV
                     RANSVATNAPPHGAFRGFGAPQSLFAMERHMDFIAQTLGISPIEVRRRNFLKPGQTTT
                     TEQVIQEQIDLGKLLDRALELADYQSKKKTFAQENLTGDKKRGIGIAAFLHGAGFTGS
                     GERYLNSLVGVETCPDGTVRVLVSSTEFGQGTKTVLCQIAAEALGVRYEDVGIAQPDT
                     QKVPNSGPTVASRTIMVVGKLVHSAALAILQTLRSSGLLGKIYSPEEFRAACRQHVAQ
                     HGVFRSLARYQQADDISWDEENYRGAAYAAFAWAVYIAEVTVDLTSYNVTVDDFVCLQ
                     EVGRVLHPVLAA"
BASE COUNT          540 a          621 c          656 g          503 t
ORIGIN      
        1 gaatgaagcg ctttgcagaa gggaattttc aaacggcatg gggcggtatt ccgagtttgt
       61 ctatcgcgtt gctagtcatg tataccgaag ctcgggcgcg gggattttcc ctcgcagaca
      121 ttgcgcgctg gatggccgaa gcgcctgcga cgctggccgg ctgccaactt cacaagggca
      181 agattgccgc aggttacgac gccgacctgg cagtttttga tgagtctagc gaatttctgg
      241 tgacagctga ccgccttcac caccggcatc cgttctcgcc gtacctggac gaaaaattga
      301 agggcgaggt gaaagctacg tatgttcgcg gtaggctcgt tttcgcggat aaggcattac
      361 agggaaatcc ccaggggcgc gagtttcctg ctcaaggtcg cggatcgatc atatgagcaa
      421 cgatcgcatc gtcggcaagt cggtaccgcg gctcgaaggc cgcgacaaag tcactggcac
      481 gtcccgttac gtcgacgata tggaattgcc agacatgctt tacggcgcca ccgttcgcag
      541 ccgtatcccg cgcggcaaaa taaaatcgat tagttttcgt cccgtggtga attgggatga
      601 gtttgtcgtc gttaccgcga aagatattcc cggaaaaaat tgcatcgctc taattggcga
      661 cgatcagccc tgccttgcgg aagaattgat caatcatccc gaagaaccca ttcttctcct
      721 ggcgcaccca gaccgaaatc ttctggctaa agcggttcaa gcggtttcca tcgaatatga
      781 tccgcttccg cccatcttca ctatcgagca gagtgagacc ctttctgaaa ttatctgggg
      841 tgcggataac attttcaaaa gttacctcat tgaaaagggc gacgtagaaa gcgcgttcaa
      901 gaacgccgac tacatcgtcg aagccgaata caccaccggt gcgcaagagc agttgtacat
      961 tgagaacaac ggaatgatcg cgagtttcga tccgcaacaa ggtgtcaccg tgtggggctc
     1021 gttgcaatgt ccctactatg tgcataaggc gctgatggaa ttatgtggct tgccgggaga
     1081 ccgcgtgcgc gtggtgcaaa tggaaaccgg cggcgcattt ggcggcaagg aagaataccc
     1141 gtcgatgcta gcggcgcatg cgacgcttct ggctatcaag tccggcaagc cggtaaaaat
     1201 tgtgtacgac cggatggaag acatggccgc taccaccaag cggcatcctt cacgcacgcg
     1261 ccaccgaacc gccgtgagca gagaaggcaa aattctcggc ggagaaattg atttcaccat
     1321 tgatgggggc gcatataaaa ccctgacgcc ggtggtgctt tcgcgcggtg cactgcacgc
     1381 tggtggaccg tattactggc ccagcgttcg cgttcgcgcc aattcggtgg ccaccaatgc
     1441 gccgccgcac ggcgcatttc gcggatttgg cgcgccgcag tcgctcttcg cgatggagcg
     1501 tcacatggac tttatcgcgc agactctggg gatttctccc atcgaggttc gaagacgaaa
     1561 ttttcttaag cccgggcaaa cgaccaccac ggaacaagtc atacaagaac aaattgatct
     1621 ggggaagctt ttggatcgtg cgctggaact ggccgactac cagtcaaaga aaaaaacttt
     1681 cgctcaagaa aatctaaccg gggacaaaaa gagaggaatc ggcattgccg ctttcttaca
     1741 tggtgcgggt tttaccggtt ccggcgaacg ctatctaaat tcgcttgtcg gcgtcgagac
     1801 ctgccccgac ggcaccgtac gcgtgttggt gtcgagtacc gaatttggcc agggcacaaa
     1861 aacggtgctc tgccagatcg ccgcagaggc actgggagtg cgttacgaag atgtaggcat
     1921 cgcgcaaccg gacacccaaa aagttccgaa tagcggaccc acggtggcat cgcgaaccat
     1981 catggtggta ggaaaactgg tccattcagc ggctttagca atcctgcaaa cgctaaggtc
     2041 cagcggtttg ctgggcaaaa tttattcgcc cgaagagttt cgagcggcct gccgccaaca
     2101 tgtcgcgcag catggagtat tccgttctct cgcgcgttac cagcaggctg acgacatttc
     2161 ctgggacgaa gaaaattatc gcggggccgc ctatgcagcg tttgcgtggg ccgtttacat
     2221 tgcagaagta accgtcgacc tcacttccta caacgtcacc gtggacgatt tcgtttgtct
     2281 tcaggaggtc ggcagagttc ttcatccggt tctcgccgcg
//
LOCUS       DATCBB010001038         1338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_259219, whole genome shotgun sequence.
ACCESSION   DATCBB010001038 DATCBB010000000
VERSION     DATCBB010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259219"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VII37_11330"
     CDS             complement(<1..777)
                     /locus_tag="VII37_11330"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEY5070380.1"
                     /translation="MPRHARRNTFLSWPVELFAVAAIFFFSAASVPQLHAQQRREREP
                     NSVYAERRAKIAAQADGPVVLWGFTGREEVSQAYVFTQEDNFYYLTGHNEEGAGLVIL
                     PAPKPGAAANSDPLNGVREILFLPAKDPAKEKWNGVRMSPTDPDIAARTGFATVKPFA
                     EMRAEVEKLAKNFPTVYTILPYEKELGGYPHEKALIDWLQIAAPQAKVKDIRQYIYSL
                     RQIKSQGEITFLQKAIELSLDAQLEAMQMMRPGLYEYQVAA"
     gene            complement(1008..>1338)
                     /locus_tag="VII37_11335"
     CDS             complement(1008..>1338)
                     /locus_tag="VII37_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5070381.1"
                     /translation="LVSAWDFVLDDWLHDRMAVLRGVEDGFPIARAAKQGLLTVSDDR
                     GRILAQGRSTEKPFSYLIATVPVRHDSTLYARWGNWFAWLNIVTMLLAFFTAWQKSKE
                     AVPKAEP"
BASE COUNT          290 a          373 c          384 g          291 t
ORIGIN      
        1 tgcggcgact tggtactcgt acaggcccgg ccgcatcatt tgcatggctt ccaattgcgc
       61 gtccagggaa agttcgatgg ctttttgcag aaacgtgatt tcgccctgcg atttaatctg
      121 acgcagagaa taaatatact ggcgaatatc tttcactttg gcctggggcg cggcaatctg
      181 gagccagtcg ataagagctt tttcgtgcgg gtagccgccc agttcttttt catagggaag
      241 aatggtgtaa acagtaggga agttctttgc cagcttctcg acttcagcac gcatttcggc
      301 aaaaggcttg acggtagcga atccggtgcg tgcggcgata tccggatccg tgggcgacat
      361 gcgcacgcca ttccattttt ctttggcggg atctttggcg ggtagaaata aaatttcgcg
      421 cacgccattc agcggatcgc tgttggcagc ggcgcccggt ttcggcgcgg gaagaatgac
      481 gaggccggcg ccttcttcgt tgtggccggt gagatagtaa aaattatctt cctgcgtgaa
      541 aacgtaggcc tgcgaaactt cttcacgccc ggtgaagccc cacaagacca ccggaccgtc
      601 ggcctgcgcg gcgattttgg cgcggcgttc ggcgtaaacg ctgttgggtt cgcgctcgcg
      661 gcgctgctgg gcgtggagtt gcgggacgct cgctgcgctg aagaaaaata tcgccgcgac
      721 ggcgaagagc tcaaccggcc acgacaaaaa tgtattccgc cgggcatgcc gcggcattct
      781 agataaattc ggcacagaaa tattcactcg cgcgccagtc ggctgtccac tcaacggatt
      841 ctcctcgaaa taaccaccaa tgcgcccggg gcaagcgggt tttgcaacag ccaggcgcgc
      901 tgtctgcaaa cgtcagaccg ccgccaggac cagcggcgag caacacgttt tcagtgcgcc
      961 tgatgacgcg attgtagccg aattcttcct gacgccaaac gccggaatta tggctcagct
     1021 ttgggtacgg cttctttcga cttctgccat gcggtgaaaa atgcgaggag catcgttacg
     1081 atgttcagcc aagcgaacca atttccccaa cgggcgtaca aagtcgaatc gtgacgtact
     1141 ggcacggtag caattaaata cgaaaacggt ttttctgtgc tccggccttg cgccaggatg
     1201 cggccgcgat cgtcgctaac ggtcagcaat ccctgctttg ccgcgcgcgc gatgggaaac
     1261 ccgtcttcca cgccacgcag aacggccatt cgatcatgca gccaatcgtc caaaacgaaa
     1321 tcccatgcgg acacgagc
//
LOCUS       DATCBB010001039         1009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_389155, whole genome shotgun sequence.
ACCESSION   DATCBB010001039 DATCBB010000000
VERSION     DATCBB010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389155"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VII37_11340"
     CDS             complement(<1..223)
                     /locus_tag="VII37_11340"
                     /inference="COORDINATES: protein motif:HMM:NF042623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HEY5070382.1"
                     /translation="MSAATHLYRRKAVYYWRRRLPNALASWFHKRHLFLSLQTPDPNF
                     ARRLVVLLDAKLEEVVTAFEHTEMHLTPPQ"
     gene            complement(578..>1009)
                     /locus_tag="VII37_11345"
     CDS             complement(578..>1009)
                     /locus_tag="VII37_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEY5070383.1"
                     /translation="SQAQKTLGTVSLEDCEIYANAEPCVFCCYAIRESRIRRVVYGLS
                     SPHMGGVSKWNVLGDTDISSAMPEVFAPPPEIVSGYMAKDAEQALIDWNPLIAEIIRQ
                     RGLFGAAPRVITEVMHEQAPGLRGRVLRFLRRNLFDYFGRR"
BASE COUNT          222 a          283 c          290 g          214 t
ORIGIN      
        1 cctggggcgg cgtcagatgc atctcggtat gctcgaaggc cgtcacgacc tcctccaatt
       61 ttgcatcgag caggaccacc agacgccgcg cgaaattggg atcgggtgtt tgcaagctca
      121 ggaacagatg acgtttatga aaccagctgg cgagcgcgtt cggaagacgg cgccgccagt
      181 agtacacggc ttttcttcga tagagatgag ttgcggctga cacgatcgct ccgtgaagca
      241 gtcatgtgtt gcacgctgtg gctcagcgcc ccacctaggc cgaattattc aatgttttca
      301 gtatcttgcg tttttctggc tggcgacaca gtgctgatcg cacccgtctc cacgcgaatt
      361 ccctgctaac agggaatttt acagggaatt tttcaattcc ggggccctaa agggcaattc
      421 ctctgcgaga aactactgtg ccgcagcagc ttttcgcaca tttccctact caatttaaca
      481 gggaaaagat ttgcaagaac agggaatttt ctagcgagaa cagggaaatt cgcagcaaag
      541 atcaccgggt gaggttttcg gtacacacac gggacagtca gcggcggccg aaatagtcaa
      601 agaggttgcg gcgcaggaat cgaagcacgc gaccgcgaag gcccggagcc tgctcgtgca
      661 tgacctcggt gatgacccgt ggtgccgcac caaacaggcc gcgctgtcta atgatctcgg
      721 cgatcagtgg gttccagtcg atcagcgcct gctctgcgtc tttcgccatg tacccggaga
      781 cgatctctgg gggaggcgcg aacacttccg gcattgcact ggaaatgtcc gtgtcgccca
      841 gaacgttcca cttcgacacg ccgcccatgt gcggggaact caagccgtag accacgcggc
      901 ggatgcggct ctcgcggatc gcgtagcagc aaaagacgca aggctccgca ttcgcgtaga
      961 tctcgcagtc ttcaaggctc acggtaccga gcgttttttg cgcttgcga
//
LOCUS       DATCBB010001040         1304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_389241, whole genome shotgun sequence.
ACCESSION   DATCBB010001040 DATCBB010000000
VERSION     DATCBB010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389241"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1304)
                     /locus_tag="VII37_11350"
     CDS             complement(<1..>1304)
                     /locus_tag="VII37_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5070384.1"
                     /translation="SLVKEDARETDIIGWLDTVAGDLRYATRVLRKNPGFALTAILTL
                     ALGIGANTAFFSVVNGVLLNPLPYPQPEQLVTMHESKPNFPSGSISYSNFFDWQKNNR
                     SFSSMAVSRGYGFSLTGLGDAEQLDAKFISSDYFVVLGANPVIGRTFAPGEDKIGAAP
                     IALIGAGFWKRKFGSSGDVLGKSITLDGKSYTIVGVIPEAFDLYRSSHATDLYVPIGQ
                     WSNPLLPNRDAGLGIHGVARLKAGVTIEQARGDMERVTGNLATIYPDADKGIGAQVVP
                     FRESLLGKVEPILLVLLGAVGFVLLIACFNVASLMLARSTTRTREFAVRVALGAGRSR
                     LIRQILTESILLSLMGGALGLLLAQWITHAALRLLPVEMPRAADIHLDSHVLIFTAAI
                     SLLAGIVFGLAPALKSSDLFLSETLKESGRGSSGTQHRAQSV"
BASE COUNT          336 a          378 c          333 g          257 t
ORIGIN      
        1 aaacactctg agcgcgatgc tgcgtcccgc ttgaaccccg cccgctttcc ttgagcgtct
       61 cagacaaaaa aagatccgaa gattttagcg cgggcgccag tccgaacact atcccggcta
      121 ataaagaaat cgcagctgta aaaatgagca catgcgagtc cagatgtatg tccgcggcac
      181 gaggcatttc caccggcagc aaccgcagcg cagcgtgggt gatccattgc gcaagtagca
      241 gccctaaagc gcctcccatc agcgaaagca aaatactctc agtaaggatc tggcggatta
      301 atcgcgagcg tccggcgcca agcgccacgc gaactgcaaa ttcgcgcgtg cgcgtagtgg
      361 agcgcgccag catcaggcta gccacattaa aacacgcgat gagcagtaca aatccaaccg
      421 cgccaagcag caccaacaaa attggctcaa ccttaccaag caggctttcc ctgaagggca
      481 caacctgtgc gccgatacct ttgtcggcat ccggataaat cgtcgcgaga tttccggtga
      541 cgcgctccat atcgccacgg gcttgttcga ttgtgactcc agctttgagc ctcgctaccc
      601 cgtgaatccc caggcccgcg tcgcggttcg gaagtaatgg attgctccac tggccgatcg
      661 gaacatacag gtccgtcgca tgggagctgc gatacaaatc gaaagcttcg ggaatgactc
      721 ctacgatggt gtagcttttg ccatccagcg tgatgctttt tccgagcacg tcaccagaag
      781 aaccgaattt acgcttccag aatcccgcac ctatcaacgc gatgggtgct gcgccgattt
      841 tatcttcgcc aggagcaaaa gttctgccga tcaccggatt cgcgccgagc accacgaagt
      901 aatccgacga gatgaattta gcatccaact gttcggcatc gcccaggccc gtcaggctga
      961 agccatagcc gcgagaaaca gccatggaag aaaaagaccg gttgtttttc tgccaatcaa
     1021 aaaaattgga ataagaaatc gagccgctgg gaaaatttgg tttgctctcg tgcatggtaa
     1081 ccagttgctc cggctgcggg tacggaagcg gattgagcag cactccattc acaacagaaa
     1141 aaaacgccgt gttggccccg atccccagag caagagtcaa aatcgccgtg agcgcgaaac
     1201 ccggattttt gcgtaatact cgtgtggcgt aacgaagatc tccagcgaca gtgtccagcc
     1261 agccgataat atcggtttcc cgcgcgtcct cttttacaag cgaa
//
LOCUS       DATCBB010001041         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U7661
            k141_453989, whole genome shotgun sequence.
ACCESSION   DATCBB010001041 DATCBB010000000
VERSION     DATCBB010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962037
            Sequence Read Archive: SRR3985384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:58:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,272
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,262
            CDSs (with protein)               :: 2,262
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453989"
                     /isolate="SMAG_U7661"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VII37_11355"
     CDS             complement(<1..921)
                     /locus_tag="VII37_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5070385.1"
                     /translation="MIFDDLRTIQTRHGFLPKGELESLSQRTQTPLYQIHSVASFYPH
                     FHLAPPPKADVRVCADMSCHLNGASELRSALQSSFVNSRPEDVQIRDVSCLGRCDHAP
                     AISLNDYIFTGVTASMAEQLTRATLRGELLQDLSHASPSAVCKSDPYAGPDAAGEKYA
                     VTRSLVQTLNWDGVIASLKESELRGMGGAGFPTSLKWDLVRKQPDPVKYVVCNADESE
                     PGTIKDRFILTHIPHLVIEGMIVAGLVTGAKKGILYIRHEYHAQEEILRTEIAGCYAE
                     RLLGKNILGSALAFDLEVFVSPGGYICGEET"
     gene            1223..>2072
                     /locus_tag="VII37_11360"
     CDS             1223..>2072
                     /locus_tag="VII37_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY5070386.1"
                     /translation="MPNFIGAAENESAKYIGPGSCAATSCHGSVKPVADSRILQNEYS
                     TWIIKDKHSKAYQALTGDIGERMARILKLGSKAEDAPKCLACHALYTTPAQRGRPFEI
                     AEGVSCENCHGPSSAWLGPHTERQWTHEKSIALGMVDTRNVIHRTEKCLECHLGSQNK
                     FVDHEMIAAGHPDLFFELDSFSAVEPRHWKTPRESAPGKPVEDGAWSGVREWSTGQAV
                     QLRASLERLSWRAKNERYDKKDVWPEYSELSCFACHHALVPAKDSWRQAHEFSGRRPG
                     DPAWNES"
BASE COUNT          487 a          535 c          595 g          455 t
ORIGIN      
        1 ggtttcttcg ccgcaaatat atccgccggg gctgacaaaa acttccagat cgaaagcgag
       61 cgcgctgcca agaatatttt ttccgagtag cctttcggcg tagcatccgg cgatctccgt
      121 acgcagaatt tcttcttgcg cgtgatattc gtgacgaatg tagaggatgc ccttttttgc
      181 cccggtgacc aggcccgcga cgatcatccc ttcgataacc aggtgtggaa tgtgcgtgag
      241 gatgaagcga tctttgatcg tgccgggctc gctctcgtcc gcattgcaaa caacgtattt
      301 caccgggtcc ggttgtttgc gaaccagatc ccatttcaga ctagtcggaa agccggcgcc
      361 gcccatgccg cgaagttcag attctttgag cgaggcgata acgccatccc aatttagcgt
      421 ctgcaccaag cttcgcgtca ctgcgtattt ttctcccgcg gcgtcagggc ccgcgtacgg
      481 atctgatttg caaaccgcgg atggcgaagc atgtgaaagg tcctgtaaca gctccccgcg
      541 aagcgtggca cgggtcaatt gttcggccat cgaggcggta acacccgtaa aaatgtagtc
      601 gttcaaagaa attgccggtg catgatcgca gcgtcccagg cacgatacgt cgcgtatctg
      661 cacgtcctcc ggacgtgaat taacgaagga cgattgcaga gcggagcgca attcagacgc
      721 gccgttcaag tggcagctca tgtcggcgca aacacgcacg tcagctttag gcggaggagc
      781 taaatgaaaa tgcggataaa agcttgcgac gctgtgaatc tgatacaagg gtgtctgcgt
      841 tcgctgcgaa agagactcga gctctccctt cggcagaaaa ccgtgacgag tttgaattgt
      901 gcgaaggtcg tcgaagatca ctgcgcccgt ctcaatgtct ttacgccgct gtattttaag
      961 aaacgacgtg attggagtgg attaatcgta tgcatactac ttactgagcg ggcaggtgta
     1021 aatagcgcct ctttggtttt tcggcgcaag ggccccaaaa gtcaactcgg ctcgttgacg
     1081 aattgccaca gctgaggata taagcgaaca gatttattcg aacattgtaa agccctaaat
     1141 gcgtcaaaag cgccgaatgt ataggtcagt ctatttcgct caccgtgttg gctggtgtgt
     1201 tgcactgctg acgttaagcg cgttgccaaa ttttattggt gccgcggaga atgaatcggc
     1261 caagtacatc ggcccgggct cttgcgcggc caccagttgc cacggcagcg taaagcccgt
     1321 tgccgatagc cgcattctgc aaaatgaata ctccacctgg attattaagg ataagcacag
     1381 caaggcgtac caggcgctga ccggggatat tggcgagcgc atggcgcgca tcttgaagct
     1441 gggttccaaa gcagaagacg cgccgaaatg tttagcctgc catgcgctct ataccacgcc
     1501 cgcccagcgc ggacggccgt ttgaaattgc ggaaggagtg tcctgcgaaa attgccatgg
     1561 tccgtcgtcg gcatggctag ggcctcatac cgagaggcaa tggacacatg agaaatccat
     1621 cgcgctggga atggtggaca cgcggaatgt gattcatcgc accgaaaaat gcctggagtg
     1681 ccacctgggc tcgcaaaata agtttgtaga ccacgaaatg atcgccgcgg gccatccaga
     1741 tctttttttt gagctggatt cattttcagc tgtagaacca cgtcactgga agacgccgcg
     1801 cgagtcggcg cctggcaagc ccgttgagga tggcgcgtgg agtggagtgc gggaatggag
     1861 caccggtcag gcggtacaat tgcgcgcttc actcgaaaga ttgagctgga gagccaagaa
     1921 cgagcgctac gacaaaaaag atgtctggcc ggaatattca gagctctcgt gcttcgcgtg
     1981 tcatcacgcg ctggttccgg cgaaagacag ttggcggcaa gcacacgaat tttcggggag
     2041 acgccctggc gatccagcgt ggaacgaatc tc
//
